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<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A182KYI1</accession>
    <name>A0A182KYI1_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Leptin receptor overlapping transcript-like 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the OB-RGRP/VPS55 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_049465846.1">
        <property type="nucleotide sequence ID" value="XM_049609889.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A182KYI1"/>
    <dbReference type="STRING" id="1518534.A0A182KYI1"/>
    <dbReference type="EnsemblMetazoa" id="ACOM029097-RA">
        <property type="protein sequence ID" value="ACOM029097-PA.1"/>
        <property type="gene ID" value="ACOM029097"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON008693-RA">
        <property type="protein sequence ID" value="ACON008693-PA"/>
        <property type="gene ID" value="ACON008693"/>
    </dbReference>
    <dbReference type="GeneID" id="120959016"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002437"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACOM029097"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008693"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032705"/>
    <dbReference type="Proteomes" id="UP000075882">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032511">
        <property type="term" value="P:late endosome to vacuole transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007262">
        <property type="entry name" value="Vps55/LEPROT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12050">
        <property type="entry name" value="LEPTIN RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12050:SF0">
        <property type="entry name" value="RH04491P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04133">
        <property type="entry name" value="Vps55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="82"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008693-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="116" mass="12637" checksum="55A4A045921F6DED" modified="2016-09-07" version="1">MLAMLGSIGMTMLILACALPTYNLWWPIFVVLFYILCPFPTLIAKRIESDDPARAASAMFATIGIVMSSFALPIVLARAEVIQWGACLLTLAGNVGAYATILAYYFGFESGDSNMW</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>A0A182LKP2</accession>
    <name>A0A182LKP2_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040219672.1">
        <property type="nucleotide sequence ID" value="XM_040363738.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A182LKP2"/>
    <dbReference type="STRING" id="1518534.A0A182LKP2"/>
    <dbReference type="EnsemblMetazoa" id="ACOM039598-RA">
        <property type="protein sequence ID" value="ACOM039598-PA"/>
        <property type="gene ID" value="ACOM039598"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON006508-RB">
        <property type="protein sequence ID" value="ACON006508-PB"/>
        <property type="gene ID" value="ACON006508"/>
    </dbReference>
    <dbReference type="GeneID" id="120947912"/>
    <dbReference type="CTD" id="39415"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005227"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACOM039598"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON006508"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035548"/>
    <dbReference type="Proteomes" id="UP000075882">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000003">
        <property type="entry name" value="Putative calcium-binding mitochondrial carrier protein scamc-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002167">
        <property type="entry name" value="GDC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24089">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00928">
        <property type="entry name" value="GRAVESDC"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="50"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="142"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="237"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON006508-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="338" mass="37637" checksum="FD0E32AE33257C43" modified="2016-09-07" version="1">MAFEQNTLQVSGDGLDDFILVFKELLSRYLDIGEDLNVPDDFTQSEMQTGMWWRHLAAGGIAGAVSRTCTAPLDRLKVFLQVQASKQRISDCLQYMLKEGGVRSLWRGNFINVLKIAPESAIKFAAYEQVKRLIRGNDKRQMTIYERFVAGACAGGVSQTAIYPMEVLKTRLALRKTGEYSSILDAASKIYRREGLRSFYRGYIPNMLGIIPYAGIDLAVYETLKKKYLSHHETEQPSFWLLLACGSASSTLGQVCSYPLALVRTRLQAQAVTIGPNPDGSVAVEPNMTNVFKRIIQTEGPVGLYRGITPNFIKVLPAVSISYVVYEYTSRALGVNMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8UXW5</accession>
    <name>A0A6E8UXW5_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">F-actin monooxygenase</fullName>
            <ecNumber evidence="4">1.14.13.225</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-methionyl-[F-actin] + NADPH + O2 + H(+) = L-methionyl-(R)-S-oxide-[F-actin] + NADP(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:51308"/>
            <dbReference type="Rhea" id="RHEA-COMP:12953"/>
            <dbReference type="Rhea" id="RHEA-COMP:12956"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16044"/>
            <dbReference type="ChEBI" id="CHEBI:45764"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.14.13.225"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Mical family.</text>
    </comment>
    <dbReference type="EC" id="1.14.13.225" evidence="4"/>
    <dbReference type="EnsemblMetazoa" id="ACON000222-RA">
        <property type="protein sequence ID" value="ACON000222-PA"/>
        <property type="gene ID" value="ACON000222"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009274"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000222"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038346"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120501">
        <property type="term" value="F:F-actin monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="LIM_Mical"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="protein-methionine sulfoxide oxidase Mical isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000004">
        <property type="entry name" value="protein-methionine sulfoxide oxidase MICAL2 isoform X1"/>
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    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="bMERB_dom"/>
    </dbReference>
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        <property type="entry name" value="CH_dom"/>
    </dbReference>
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        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="F-actin_Monoox_Mical"/>
    </dbReference>
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        <property type="entry name" value="FAD/NAD-bd_sf"/>
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        <property type="entry name" value="Rossman_Mical"/>
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        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
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        <property type="entry name" value="[F-ACTIN]-MONOOXYGENASE MICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23167">
        <property type="entry name" value="CALPONIN HOMOLOGY DOMAIN-CONTAINING PROTEIN DDB_G0272472-RELATED"/>
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0274">FAD</keyword>
    <keyword evidence="6" id="KW-0285">Flavoprotein</keyword>
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    <keyword evidence="12" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="10" id="KW-0521">NADP</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="527"/>
            <end position="634"/>
        </location>
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            <begin position="1041"/>
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            <begin position="2933"/>
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    <evidence type="ECO:0000256" key="17">
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            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="PROSITE" id="PS51848"/>
        </source>
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    <evidence type="ECO:0000313" key="21">
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            <dbReference type="EnsemblMetazoa" id="ACON000222-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="3109" mass="339666" checksum="12A3D0D2264EF6B3" modified="2023-06-28" version="2">MSEQFTLDHERAAMATEMFLHFCAATTMKQIRGLYWNMLDTIGLRPGPLEEFYPKMKAAIRDWRAQALFKKFDARAAHKVYCKGRAASKTRVLIVGAGPCGLRTAIDAQLLGAKVVVVEKRDRISRNNVLHLWPFIIHDLKALGAKKFYGKFCAGSIDHISIRQLQCILLKVALLLGVEMHEGVSFVKEIEPGDGYGWRASVSPEDHAVSHYEFDVLIGADGKRNTLEGFQRKEFRGKLAIAITANFINKRTEAEAMVEEISGVAFIFDQPFFKALYEKTGCDLENIVYYKDDTHYFVMTAKKHSLLHRGVIIKDLSDPAELLAPSNVDKPKLYEYARDAANFATKYQMPNLEFAVNHYGTPDVAVFDFTSIFAAHNSCKVTVRKNYRLLSCLVGDSLLEPFWPTGSGCARGFLSSMDAAYAIKLFANPKNSLLATIAQRESVYRLLGQTTPENLNRAFGAYTLDPSTRYKNLNKASVQIGQVKHLLDTDDPALLEQTFFDTNAIASAVSPATELPAKRKRRTVDVVPLGATLLRWLKAHLKDSGFVQELAEPAECFTNGKVLCTLIHRYRPDLVNLEELADFSADVCNEHAFNIFETQLGIPRVMSGQESVTLAGVEPKVWLNYLEQVCDVFRGEVPHVKHIKLDYAEFKQKQETNVEAWSKLGRMAAVQRMAALASGVHPADKDGELLHRGTKGDCIVPVATASGPYGGGANAGAAAARPNAKNYYHPNAEEERYKRGVGGVASPQNVTMRRMRKRRSNEKSGNIEERTRRLQEIEANRQDRQSKRREQRAQQTQNFYKSIHMLQANTLLRESDNAGAFEDYSLYLYRQSAPVFTDRVRELEQKLRYPDRDRGLAAAYPRGLADASADQFSDRVRSMEQRIDGRTVPVVEKKPKDLLRAIGKIDSSDWNVKEIERKIEQSKKTTDIGKSRERVPNWNREQFLARQNRLMQPQDDEKYREIDSSLKAIDKQLKEGHNLDLGERGKNKVALIAGQLQTPNKPGKQQQQQQQQQQQQQQQQQQQAPKSNQKALVLTASGVAEKCHYCKQRVYLMEKISAEGLTLHRSCLKCHHCHTILRLGAYAFDRDDPEGRFYCTPHFKLPAKTLKPAAGKAKGRAGAAAAAVAPVIGRMYDASDAGGGGGGVMVRCGPIGTAGGSAGETGGVGASTVDYLARNRTPDNDDSDGDPNMEVIDENEWTDRNFGTESEVSEAELSSTDESDTDSESDYEETADSPLGAQTLQLASEWISDKRFSNLVDSDADFYSYSSEDDDADSQTEGEELARAREMRRQEVKLMPPPTLPTDTETEGASNSEVNSTEISTDSEFDDEQPQRVPPSFHLEFKPLVEVDPTIKLVQNRAPLAVPRPGDYVLSKTASTEGIASKKSLELKKKYLLGEGTTGLGILKSGSASALDSKFKTFHSNITECQKLLNPAAAVQVSPTLQPFLKASAPAVPVPAAPVAPGPTESANDTEKENVYEGKRSGNGQPNVVGSVVAGAANKRSSLVETINTTLVENKLNEIKSSECFPALPPKEEPPEQPPVTSPSQNGSPAKPSESNGHLDHAAKLKNSINNNNHSSNGTQNNNNNHHHHHHHHTPVNSKANNHNNNNNNNNGEEEEEEVEVIEIIDIVTPEKGKATTASPTHREVASSGATHEPVAGPERKYIGALKHAYIDLTTDSPNCDRSLVETTLDFSIAPSKVAGAIVPERPTNSTAVPDAASGNARNDSNDNKIEQTANGSSAEQQAPKEQQRAVEEEREQEEGCHETTLQVPLDVPWTKQEEIESDSISESGSNSSGISSSSSSSSSSSSSTSSVEDIPHFILDSTTSPETQHGDERFVPRLEVRDATGELMQIDSLMIIDGRYIGDPEDLKLMERLPPDTQIAAQLLQAEMNQDSISTVVESAPEHPLQEERKPSADEQVVPAEEQEEEEEEEERASTPTPPAKPEEPVAEVHAAATTTTAPSLSNFVSQRTGGFRFDSLHEHKLDTLKNLPFVLEAQRSAVPSKPTHLCLGEPRPPESETDAEQTPMAGEMLPPLVAVAAATQDPDSDSPDDTEVTTQNNLTETELSDWAADDAVSENFVDLEFGLGAGRGATVRRNQKPKHLRLPAKRDGGANARGAVLPLAATAPLATEDGIIRNLALEDIEFMDTGSEEESCIETYSTTNRMVVKNRGYMEIMDPAVAAQRTVYHPPPPVYGTAAPLVEAINSQVAGRQLDYIEQGAYLLATDDAKTPMNEEPPGKGTFPGACPGLRLDADIEEDSLLLVSSSQGTGTATDSTYGDSDATTIVGPAHLEASGRPLAGGGAAVASPKPSAGSSGRGSAGDVEEEDQQEEQGDGPARKISVESLSGKRSSLERKDVRRDSLKSIEDIGYEKYVKRLQQKIQQISKARDSLEVMKSAKRKSPKADGSTVVLPLPEAVPPATAESVPGASPKADPPQQQQQSPQSPQSPQSPAPAGTPEPPKSVEKKIEEIAKERVKQKDLIHDLVMDKLQTKKQLNAEKRLNRSRNRSNALGGQQSAGVADVPPAVDGSSPARAAGHPLNGGPAAPVTPVALVTPRAGGEAGPTTGQLREEARSRARLKSNQDLGLSPEDQVLLLRKKYHLTMRALTNTTTTTDDDDDANNRAEPNKSDDAKCKDQQQQQQQQHHRTKLITSKSVNDVSMLKQQQQHHHHHHHYNQAALAHHHHHHLHHQHSQQPSYRITSTGLGAATGELADPPPLPHKLTDYISDPNLVHSGATEPDSEVRVRGAATKRGKDRERRKSIIEKVSEFFNGRKKPTAPDGAGGSKEASPTKDRPPSNLLAVNGQPEPVNGGGSTSPSSGGGFLRFKISPKLKDKSKTKESSPYGARCASDDCLNTNGAAATGSPTTTTPDAAGPNHHHHNSYTPIGRKEAEELEPPPIPPLPLNYERSDDEAAGAANESKNEIKKIRAMSKTSRQAELKRLRIAQEIQREQAEIDVKIKDLETRGVAIEKELRGESEALRQHGPAANLGANDEGLVKEWLEIMSSITRLKVRDDELSIRQQELQLEHRHAQLKEELNMRLSYGKLDKNSSDVAAEGAILNEMLEIVAKRQSLRPSPDTKATAAAVAAVAAGAASSPFTNPTGRKPTDTDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8UXX9</accession>
    <name>A0A6E8UXX9_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Rhophilin</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RHPN family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040223241.2">
        <property type="nucleotide sequence ID" value="XM_040367307.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040223247.2">
        <property type="nucleotide sequence ID" value="XM_040367313.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8UXX9"/>
    <dbReference type="EnsemblMetazoa" id="ACON000243-RA">
        <property type="protein sequence ID" value="ACON000243-PA"/>
        <property type="gene ID" value="ACON000243"/>
    </dbReference>
    <dbReference type="GeneID" id="120949781"/>
    <dbReference type="CTD" id="32531"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006085"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000243"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038658"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051497">
        <property type="term" value="P:negative regulation of stress fiber assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11633">
        <property type="entry name" value="HR1_Rhophilin-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.280">
        <property type="entry name" value="alix/aip1 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.160">
        <property type="entry name" value="HR1 repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004328">
        <property type="entry name" value="BRO1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038499">
        <property type="entry name" value="BRO1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011072">
        <property type="entry name" value="HR1_rho-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036274">
        <property type="entry name" value="HR1_rpt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047138">
        <property type="entry name" value="RHPN1_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23031:SF15">
        <property type="entry name" value="LD12055P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23031">
        <property type="entry name" value="RHOPHILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03097">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02185">
        <property type="entry name" value="HR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01041">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00742">
        <property type="entry name" value="Hr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46585">
        <property type="entry name" value="HR1 repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51180">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51860">
        <property type="entry name" value="REM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="REM-1" evidence="7">
        <location>
            <begin position="13"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="BRO1" evidence="6">
        <location>
            <begin position="98"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="5">
        <location>
            <begin position="668"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="355"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="539"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="628"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="756"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="64"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="363"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="557"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="567"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="631"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="756"/>
            <end position="784"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000243-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="817" mass="89744" checksum="DD7237D8EC71CF50" modified="2023-06-28" version="2">MGFLQDLQLKGSDPRVATCRGKLQSKRCKLNQEINKELRLRAGAENLYKATTNKKLKDTVALELSFVNSNLQLLKEQLSELNSSVEIYQSEGLDYVIPMIPLGLKETKEVNFMEPFSDFILEHYSEPSHIYEDAIADITDTRQAAKTPTRDAQGVSLLFRYYNLLYYVERRFFPPDRSLGVYFEWYDSLTGVPSCQRTVAFEKACILFNLAAIYTQIGARQDRSSEKGLDAAVDNLLRAAGVFRHIFDTFTNAPSMDLKPQVLEVLVALMLAQARECLFEKLLLQVENLPPGRDCVQNHRDLSGEATQLALEYREIHQIIHSNDAHTYLPECWAGLVPLKSEYYKALAHYHAAKTKVSGPGGTSSTAEDATATNRCNKQQQQQRYGRGLEPKDTFIFDETSFESDLDPVTLRRAHLRESLSSHEEAQRLQRMCRELKNKVALSKVLNYAHERTAEELEELELDRTGTVGSRSHLSALDEPDLDQLDVTLNATSKFTISLTGPDFTMHRCDDPFRKLGPIAIFSARRHWTAPRSVRLQKGGPSFLLGGGTLDRRQSPYGGGAGGSSSSGGNSSSSHSSSSNNRRQQSRSGGRCCGECANKTNYYNDSRACEVCLKDVCNVSEREFRELKLQDGGGGGGGGGGGGGGGGGGGGGEQQQHGTDGTGAEDGRGDISSFGFNIRGDAPVMISTVEINSLADLGGIKEGDFIVELCGVDVKWYTLQQVLKLIRNCEHSLELKVITPMDRNYLKPMAMSHSSKGSISTLSGSSSGISSGAPSPTGTTTKSATKTRSKLGKTRLSSFTSGSWNPFRRTQSLGKIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8UXY8</accession>
    <name>A0A6E8UXY8_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Disks large 1 tumor suppressor protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the MAGUK family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8UXY8"/>
    <dbReference type="EnsemblMetazoa" id="ACON000255-RC">
        <property type="protein sequence ID" value="ACON000255-PC"/>
        <property type="gene ID" value="ACON000255"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030881"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043296">
        <property type="term" value="C:apical junction complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031594">
        <property type="term" value="C:neuromuscular junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098839">
        <property type="term" value="C:postsynaptic density membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043297">
        <property type="term" value="P:apical junction assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009792">
        <property type="term" value="P:embryo development ending in birth or egg hatching"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045197">
        <property type="term" value="P:establishment or maintenance of epithelial cell apical/basal polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043113">
        <property type="term" value="P:receptor clustering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097120">
        <property type="term" value="P:receptor localization to synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099072">
        <property type="term" value="P:regulation of postsynaptic membrane neurotransmitter receptor levels"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00071">
        <property type="entry name" value="GMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06723">
        <property type="entry name" value="PDZ1_Dlg1-2-4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06724">
        <property type="entry name" value="PDZ2_Dlg1-2-4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06795">
        <property type="entry name" value="PDZ3_Dlg1-2-4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11861">
        <property type="entry name" value="SH3_DLG-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001402">
        <property type="entry name" value="Discs, large homolog 3 (Drosophila)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.470:FF:000001">
        <property type="entry name" value="Disks large 1 isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000001">
        <property type="entry name" value="Disks large homolog 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.63.10:FF:000001">
        <property type="entry name" value="Disks large homolog 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000058">
        <property type="entry name" value="Disks large homolog 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000048">
        <property type="entry name" value="disks large homolog 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000004">
        <property type="entry name" value="Disks large homolog 4 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.63.10">
        <property type="entry name" value="Guanylate Kinase phosphate binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.470">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016313">
        <property type="entry name" value="DLG1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008144">
        <property type="entry name" value="Guanylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020590">
        <property type="entry name" value="Guanylate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015143">
        <property type="entry name" value="L27_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004172">
        <property type="entry name" value="L27_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036892">
        <property type="entry name" value="L27_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050614">
        <property type="entry name" value="Synaptic_Scaffolding_LAP-MAGUK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23119">
        <property type="entry name" value="DISCS LARGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23119:SF51">
        <property type="entry name" value="DISKS LARGE 1 TUMOR SUPPRESSOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09058">
        <property type="entry name" value="L27_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001741">
        <property type="entry name" value="MAGUK_DLGH"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="L27"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PDZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101288">
        <property type="entry name" value="L27 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00856">
        <property type="entry name" value="GUANYLATE_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50052">
        <property type="entry name" value="GUANYLATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51022">
        <property type="entry name" value="L27"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PDZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0965">Cell junction</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 11" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="L27" evidence="17">
        <location>
            <begin position="4"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="16">
        <location>
            <begin position="155"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="16">
        <location>
            <begin position="252"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="16">
        <location>
            <begin position="443"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="14">
        <location>
            <begin position="554"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate kinase-like" evidence="15">
        <location>
            <begin position="730"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="618"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="666"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="88"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000255-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="20">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="920" mass="102025" checksum="1694FAE58AF5267C" modified="2023-06-28" version="1">MPVKKQEAHRALELLEDYHSRLSTPQDRALRSAIERVIRIFKSRLFQALLDIQEFYELTLLDESKTVQQKTAETLLIASKWEQDNAIKANEANRIKQQEQEQQQQQLQQQQQQQQQAPPQLQLQQEIEKQPIIELPSPRLTTTKLNGDSPWEYDEITLIRGSTGLGFSIAGGTDNPHINLDASIYITKVIPGGAAHVDGRLQVNDCIVAVNEVRVVNVTHGEAVDALKQAGDRVTLHIRRKRPPAQAPKLEEIELLKGSKGLGFSIAGGIGNQHIPGDNGIYVTKIMEGGAAHIDGRLAVGDKLIAVRTPDGGERNLENVVHEEAVATLKAITNRATLIVQKTNILQALANSISNLNSLSTNAMNSVGVSMVDHASPDAGGGGGEAGGGDTEMVASTARSHSPAFGLENSSSRYASSNVLETGMPIGTPRAVSSEDITRVPRTIVIRKGASGLGFNIVGGEDGQGIFVSYVLAGGAADLGGELKRGDQLLSVNGISLANASHEDAAQALKNAGGTVTLVVQYRPEDYNRFEQRIQELKQAVAAGTGTLLRTTQKRTLYVRALFDYDPNRDDGLPTRGLQFRHGDILHVTNASDDEWWQARRVIGEDEEESIGIVPSKRRWERKQRARDRSVKFQGHAGANNNNNNLEKVSTLDRKKKNFSFSRRFPFMKSKDDKNEDGSDQEPNGNLSDPNHHDPDQPFMLCYTQEDANAEAEENILSYEPVQRLQISYTRPVIILGPLKDRINDDLISEYPNKFGSCVPHTTRPKRDYEVDGRDYHFIASREQMEKDIQNHLFIEAGQYNDNLYGTSVASVREVAEKGKHCILDVSGNAIKRLQVAQLYPIAIFIKPMSVESIMEMNRRMTEEQAKKTYERTLKMEHEFGEYFTAIVQGDTIEEIYSKVKNVIWSQGGPTIWVPSKESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8UY83</accession>
    <name>A0A6E8UY83_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="16">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 16">Membrane</location>
            <topology evidence="1 16">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="16"/>
    <dbReference type="RefSeq" id="XP_040225396.2">
        <property type="nucleotide sequence ID" value="XM_040369462.2"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON000390-RB">
        <property type="protein sequence ID" value="ACON000390-PB"/>
        <property type="gene ID" value="ACON000390"/>
    </dbReference>
    <dbReference type="GeneID" id="120950990"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006135"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000390"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030322"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140326">
        <property type="term" value="F:ATPase-coupled intramembrane lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000044">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000104">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000067">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000034">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006539">
        <property type="entry name" value="P-type_ATPase_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032631">
        <property type="entry name" value="P-type_ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01652">
        <property type="entry name" value="ATPase-Plipid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF175">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16209">
        <property type="entry name" value="PhoLip_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 15 16" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 14 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 16" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="58"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="282"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="331"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="1051"/>
            <end position="1074"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="1086"/>
            <end position="1106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="1137"/>
            <end position="1159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="1171"/>
            <end position="1192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="1204"/>
            <end position="1226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="1246"/>
            <end position="1268"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase N-terminal" evidence="19">
        <location>
            <begin position="24"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="18">
        <location>
            <begin position="114"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase C-terminal" evidence="20">
        <location>
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    <sequence length="1302" mass="147109" checksum="BD7DBF437FCE9EEF" modified="2020-08-12" version="1">MGNRVSQESDSLTVKVGCEVQEKKQRSQTNETNRIKSTKYTLITFLPLNLFEQFRRIANLYFLFMTVISIVIDSPVSPMTSLVPLVFVIAVTAAKQGYECFLRYRADNMVNFSPVTVIRGGVTVDIRCQDIRRGDIVLMSRDCDIPCDVVLLQSSDEHGRCYVTTANLDGETNLKTMAAPKGVPVVPPDRLHTIGRIEYERPHTDLYSFNGRIELADAYVAAGDHSVLPLATENLLLRGSRIRNTEWAIGCAVYTGQQTKLALNSRMTTSKMSSSERYVNKYLVFFLVLLVVIVTVSYFLKRYNDEYRTEHNQYLGEMLSNYRVSQFLQDFFSFLILFNYLIPISLYVTIEMAKFLGGFYLEWDPDLYDEETDQPCIVNTSDINEELGQVSLLFSDKTGTLTKNIMIFQQCSIAGRKYEQQGRRLLEAGDRRAALKINELPTQVNEFFQALAVCHTVQVAGDYTKETEEDATSPAKEAKETDVLMEESFTVGNAPNPQLAEQPTPPPDDESTVRISRRPTSGLFNGLNRVAPAVLTRSTSRPQSLVEGQTESSFILPHASGSNGNLQLPMQQLELKRTISAYEEKSAPLVHDEAKQLGHRRTQSHVPIGVNRPKSYQIALDSPTGTFQRPMVTLRRNRGSYLSRSVRESYAAPAYNESALVQRQESHLRQREVDTFIRKMDYQASSPDEKALVEACARMGLVYIGDDGDQMQIKVRRSCVRANVRSRLGEIPDEELIQYERLAVLEFTSDRKRMSIIVRDAHGQIWLYTKGAESHVLPLCAHTPLIETTQRHIDEFAKLGLRTLAVARRRLTEQQYRTFHDELIEAGSSLVDRVERVAECQQRVETELELLGATAVEDALQDDVQSTLQALGQAGIRVWVLTGDKVETALNIALSCGHIPDGAARYFITECRTAEQVQQHLDTLALEMFRQRDVPFSMLIDGASLKIALEAHREQFRDVSVRCRAVLCCRLSPLQKSEVVQLMKTAEGAPVTAAIGDGANDVSMIQEAHVGLGIVGREGRQAARCADYAFAKFCMLKKLLLVHGHYFSTRLAVLVLYFFYKNLVFMGIQLYFQIHSLFSNQSVYDSVFLTLYNVVYTAIPVLVLALTEKPYQEQTLLKNPALYQKVAGNKQYAWKYFIGWMVLGIYHFSVIYFFSYAVWIENPAIYAHWPATASFACYGTILIHNVVVLVNLKLLIESIYKSYVFIATVLLSIFAFMGSTFVYNLLNINYDGSMLQVYNNLLSSLTFWLLSFVILIAGFLPDLTMLAIRSIGVKLGHIFPGGAKYRRALFQWRGNELQSTDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8UY95</accession>
    <name>A0A6E8UY95_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 13">Histone deacetylase</fullName>
            <ecNumber evidence="3 13">3.5.1.98</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 21">
            <text>N(6)-acetyl-L-lysyl-[histone] + H2O = L-lysyl-[histone] + acetate</text>
            <dbReference type="Rhea" id="RHEA:58196"/>
            <dbReference type="Rhea" id="RHEA-COMP:9845"/>
            <dbReference type="Rhea" id="RHEA-COMP:11338"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="3.5.1.98"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the histone deacetylase family. HD type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.98" evidence="3 13"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8UY95"/>
    <dbReference type="EnsemblMetazoa" id="ACON000410-RB">
        <property type="protein sequence ID" value="ACON000410-PB"/>
        <property type="gene ID" value="ACON000410"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_008490"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000410"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040711"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000118">
        <property type="term" value="C:histone deacetylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141221">
        <property type="term" value="F:histone deacetylase activity, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040029">
        <property type="term" value="P:epigenetic regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046949">
        <property type="entry name" value="HDAC4/5/7/9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="HDACs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625:SF5">
        <property type="entry name" value="HISTONE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HDAC_II_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 13" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0678">Repressor</keyword>
    <keyword evidence="10 13" id="KW-0804">Transcription</keyword>
    <keyword evidence="9 13" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7 15" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Histone deacetylase" evidence="18">
        <location>
            <begin position="605"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="228"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="410"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="529"/>
            <end position="561"/>
        </location>
    </feature>
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        <location>
            <begin position="957"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="64"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="234"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="430"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="452"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="985"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="active site" evidence="14">
        <location>
            <position position="712"/>
        </location>
    </feature>
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        <location>
            <position position="597"/>
        </location>
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            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
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        <location>
            <position position="599"/>
        </location>
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            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
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            <position position="605"/>
        </location>
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            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00007738"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00012111"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022491"/>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00048287"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRNR" id="PIRNR037911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR037911-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR037911-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000410-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="999" mass="107911" checksum="2633C4694649D8D7" modified="2020-08-12" version="1">MARDSTMGRDRVLVVPNTGIALNSSSSSSQIAAPADISQQILELQKEQELEKQKLWHAFQEKNKELEMQHRQQLEHKFQELRDQRIAEEAAQQRERREREAIKRKEKHECCANASSEVKQKLQTFLIQKKQAAASNGLSSPLPYRNWGVVKSSSGESLPGGAVAAAVSSHPYKIPPPPSSIAKYDPDFPLRKTASEPNLLKIRLKQRVIEKRSLNGPLAARRQERLRRLQKQQHNQALAQTNCAASASVATTDSGPNSPPTVGSRASPTNAPIQEENEDPQYGSVTSRASINDLSLFSSPSMPNISLGRPHLGSSAAASVAHLALNATRPTHLGVGAGIGGQAGSVPFGSPMLDLSEQQQRSQNVAAERQAYEQHMLMKQKIRQTALTRAANCVNREPQLREEVESNEVIDLTDKKQPPKTVLASSVIKSTSQSHLLSSSSHGVDGEPPTAQQQQQQQQQHLSELIHQQQHYHRERELFMRRSIQLSVLEDPYSHHAGSAGGSGGAAAAAAAAAAAANLLRPLSRTSSSPLVHLSSIGGGGGGGRFGRRPSDGPKACSPTLGRGRAVTTICHRHREQKLRPATTGLAFDSLMLKHVCACGDNANHPEHSGRLQSIWARLLGTGLAIRCDKLRSRKATQEELQTVHTEGHALLFGTSQLNRQKVDTSRVSFVRLAIIAAAARMAAGCVIDLSYKVARGENRNGFAVVRPPGHHAEADAAMGFCFFNSIALAARLLRHRMPHEMRRILIVDWDVHHGNGTQQAFYDDASVLYLSVHRHDDGNFFPGTGGPAECGVGTGLGYNVNVAWSGGVNPPLGDAEYLAAFRTVVMPIARDFAPDIVLVSAGFDAAVGHPAPLGGYVVSPACFGHLTRELMQLANGKIVLALEGGYDLPAICDSAEECVRALLGESTSSIAPSELARPPCQAAVETLQKTIAIQVSHWPCVKRLAHTVGLSALQAHSSEREESDTVTAMAGLSMQPLKRTSDVSCEDSEEPMDQDESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V3K4</accession>
    <name>A0A6E8V3K4_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="19">3'-phosphoadenosine-5'-phosphosulfate synthase</fullName>
        </submittedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>sulfate + ATP + H(+) = adenosine 5'-phosphosulfate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:18133"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58243"/>
            <dbReference type="EC" id="2.7.7.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 1/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Sulfur metabolism; sulfate assimilation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the sulfate adenylyltransferase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">In the C-terminal section; belongs to the sulfate adenylyltransferase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the N-terminal section; belongs to the APS kinase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8V3K4"/>
    <dbReference type="EnsemblMetazoa" id="ACON001256-RA">
        <property type="protein sequence ID" value="ACON001256-PA"/>
        <property type="gene ID" value="ACON001256"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_014208"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001256"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030554"/>
    <dbReference type="UniPathway" id="UPA00097"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004020">
        <property type="term" value="F:adenylylsulfate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004781">
        <property type="term" value="F:sulfate adenylyltransferase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050428">
        <property type="term" value="P:3'-phosphoadenosine 5'-phosphosulfate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000103">
        <property type="term" value="P:sulfate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd02027">
        <property type="entry name" value="APSK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00517">
        <property type="entry name" value="ATPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.400.10:FF:000002">
        <property type="entry name" value="ATP sulfurylase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000006">
        <property type="entry name" value="bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.400.10">
        <property type="entry name" value="Sulfate adenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00065">
        <property type="entry name" value="Adenylyl_sulf_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002891">
        <property type="entry name" value="APS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059117">
        <property type="entry name" value="APS_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025980">
        <property type="entry name" value="ATP-Sase_PUA-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024951">
        <property type="entry name" value="Sulfurylase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002650">
        <property type="entry name" value="Sulphate_adenylyltransferase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00455">
        <property type="entry name" value="apsK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003013">
        <property type="entry name" value="PRK03846.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00339">
        <property type="entry name" value="sopT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11055">
        <property type="entry name" value="BIFUNCTIONAL 3'-PHOSPHOADENOSINE 5'-PHOSPHOSULFATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11055:SF1">
        <property type="entry name" value="PAPS SYNTHETASE, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01583">
        <property type="entry name" value="APS_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01747">
        <property type="entry name" value="ATP-sulfurylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14306">
        <property type="entry name" value="PUA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="7" id="KW-0934">Plastid</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="13" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="APS kinase" evidence="16">
        <location>
            <begin position="75"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-sulfurylase PUA-like" evidence="18">
        <location>
            <begin position="259"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="domain" description="Sulphate adenylyltransferase catalytic" evidence="17">
        <location>
            <begin position="424"/>
            <end position="650"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00037980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF01747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF14306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001256-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="658" mass="74067" checksum="2B56E7517D5D3DCC" modified="2020-08-12" version="1">MSCGIRHVLKCRGANILPSFGGSCSSVQRYSRPYTMQQQQQQEECLQVATNVTEQKHNVSREVRGQNLGLCRGFRGCTVWLTGLSGAGKTSIAFELEAYLVSKGIPAYGLDGDNIRTGLNKNLGFSQVDREENIRRVAEVAKLFADSGVVSICSFVSPFAEDREMARKIHKDADLKFFEIYIDTPLEVCETRDTKGLYKKARQGVIQGFTGVTQAYEAPDAPDLRVSTEGMTVRESTLKLIKLLESEAIIPKFLKEDEPLPELFVAESLKTALEAEAKTLPALALTEVELQWLQILAEGWAYPLKGFMREQEYLQALHFNCLLNEDETLRVNQSVPIVLSATEADKQRLEGVSALALQYGGRTVAVLRKPEFYYQRKEERCARQFGTSNRAHPYIKMIYESGDYLVGGEIEALERIQWNDGLDAYRLTPNELRQRFREIKADAVFAFQLRNPIHNGHALLMSDCRRQLLERGYKNPVLLLHPLGGWTKDDDVPLPVRMAQHQAVLDSGVLDRDHTVLAIFPSPMMYAGPTEVQWHAKARMNAGANHYIVGRDPAGMPHPDKDLYPDGNLYDGTHGARVLKMAPGLDSIEILPFRVAAYDKSCSQMAFFDPARKQDFDFISGTRMRTLARNGENPPNGFMEPKAWQILAEYYQSLQKSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V3M4</accession>
    <name>A0A6E8V3M4_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Inwardly rectifying k+ channel</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8V3M4"/>
    <dbReference type="EnsemblMetazoa" id="ACON001281-RA">
        <property type="protein sequence ID" value="ACON001281-PA"/>
        <property type="gene ID" value="ACON001281"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012779"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001281"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037155"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034702">
        <property type="term" value="C:monoatomic ion channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005242">
        <property type="term" value="F:inward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034765">
        <property type="term" value="P:regulation of monoatomic ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1400:FF:000001">
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        <property type="entry name" value="Putative Inward rectifier potassium channel"/>
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        <property type="entry name" value="Ig_E-set"/>
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        <property type="entry name" value="INWARD RECTIFIER POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11767:SF113">
        <property type="entry name" value="INWARDLY RECTIFYING POTASSIUM CHANNEL 2, ISOFORM D"/>
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        <property type="entry name" value="IRK"/>
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    </dbReference>
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        <property type="entry name" value="GIRK_kir"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="KIR11CHANNEL"/>
    </dbReference>
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        <property type="entry name" value="KIRCHANNEL"/>
    </dbReference>
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        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0630">Potassium</keyword>
    <keyword evidence="3 12" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 12" id="KW-0813">Transport</keyword>
    <keyword evidence="5 12" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="115"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="197"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel inwardly rectifying transmembrane" evidence="14">
        <location>
            <begin position="81"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="Inward rectifier potassium channel C-terminal" evidence="15">
        <location>
            <begin position="233"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU003822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01007"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF17655"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001281-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="426" mass="49343" checksum="81A97B518A4B27A9" modified="2020-08-12" version="1">MAAERQTLDEKKPLNRIFMRPGVIEEEPDSLDSVISNLPLTVVPSLARISTHGSAFSRQESVRSRALRPGTTRKYRRRAILKNGDCNIVLSKVSRQRLRFLQDIFTTLVDAQWRWTLLVFAFSFIGSWLFFGAIWWLIAFTHGDLEEMHLPKNQEESGWKPCVFNIESYTSAFLFSIETQTTIGYGYRTTTEECPEAIFIMCFQSIYGVMMQAFMVGFVFAKMTRPKHRTQTLLFSKHAVVCQRDGELSLMFRVGDMRKSHIIGANIRAQLIRTKLSREGEVMAQYQHELEVGADHCGSDLFFIWPQIVTHRINTESPFYNMSASDLLQDRFEIVVILEGTVESTGQSTQARSSYVNTEILWGHRFEPIVFYNKENQVYEINYSKFNETHSVDTPLCSARELAEFYKYQDDYRNQAEDHHHLQTPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V3P6</accession>
    <name>A0A6E8V3P6_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Succinate--CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrial</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8V3P6"/>
    <dbReference type="EnsemblMetazoa" id="ACON001312-RB">
        <property type="protein sequence ID" value="ACON001312-PB"/>
        <property type="gene ID" value="ACON001312"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006867"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001312"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040822"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:mitochondrion"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009361">
        <property type="term" value="C:succinate-CoA ligase complex (ADP-forming)"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:succinate-CoA ligase (ADP-forming) activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:succinate-CoA ligase (GDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Succinate--CoA ligase [ADP-forming] subunit alpha, mitochondrial"/>
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    </dbReference>
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        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017440">
        <property type="entry name" value="Cit_synth/succinyl-CoA_lig_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033847">
        <property type="entry name" value="Citrt_syn/SCS-alpha_CS"/>
    </dbReference>
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        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005811">
        <property type="entry name" value="SUCC_ACL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11117:SF2">
        <property type="entry name" value="SUCCINATE--COA LIGASE [ADP_GDP-FORMING] SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SUCCINYL-COA LIGASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00549">
        <property type="entry name" value="Ligase_CoA"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
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    </dbReference>
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        <property type="entry name" value="SUCCINYL_COA_LIG_1"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00399">
        <property type="entry name" value="SUCCINYL_COA_LIG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-citrate synthase/succinyl-CoA ligase C-terminal" evidence="2">
        <location>
            <begin position="70"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001312-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="217" mass="22943" checksum="6C7E1F65E1953A2E" modified="2020-08-12" version="1">MEAIEAEMPLIVCITEGVPQHDMVKVKHALLAQDKSRLVGPNCPGIIAPEQCKIGIMPGHIHKRGKIGVVSRSGTLTYEAVHQTTEVGLGQTLCVGIGGDPFNGTDFIDCLEVFLKDPDTKGIILIGEIGGVAEEKAADYLMQYNQGIKAKPVVSFIAGLSAPPGRRMGHAGAIISGGKGGAQDKINALEKAGVIVTRSPAQMGKELIKEMKRLELV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V413</accession>
    <name>A0A6E8V413_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_040224579.2">
        <property type="nucleotide sequence ID" value="XM_040368645.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8V413"/>
    <dbReference type="EnsemblMetazoa" id="ACON001544-RA">
        <property type="protein sequence ID" value="ACON001544-PA"/>
        <property type="gene ID" value="ACON001544"/>
    </dbReference>
    <dbReference type="GeneID" id="120950530"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_003148"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001544"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034703"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016235">
        <property type="term" value="C:aggresome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005164">
        <property type="term" value="F:tumor necrosis factor receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070842">
        <property type="term" value="P:aggresome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051865">
        <property type="term" value="P:protein autoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006513">
        <property type="term" value="P:protein monoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00121">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16619">
        <property type="entry name" value="mRING-HC-C4C4_TRIM37_C-VIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.210.10">
        <property type="entry name" value="Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002083">
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    <sequence length="504" mass="57275" checksum="7294F4A2A0ED512C" modified="2020-08-12" version="1">MASSSNRSDSRRHASSTASAHSSTITGSSSASMSSSVGATTTSYHSSMSCSPPDSPNATLSLSASSSSARDASSRRHVLSHINDIFKCTICFGRLEDPHLCPQCSKLYCYDCIGEWLDSGSSQSCPNCKIFLQLDQLVKVRWFDDIQQLQRNLRALTCGSEERDGKPGEESLVPAADLCPKHRKRVNFYCSTCEQCVCEVCATGGDGDAGQHRDHTFKALQVTYEQHVALLDGELEKVEHYRDKLSLLLDKIDRNVELIRRVKTVKHKELEAIMVAALNSLDRQEEQKLTKLRVHQFKLMTEIEEVNEKLQAMRYDMEFHSKPQLIQMKPKIVAACNAIRLLPIRDFKQIRVPASLKIEIPHLFETGVFVVQNFSTFDDNKVVYSNEFSDCMGRTWRIMAWCVISEDHFGIYLELVHGRPCWMECTFQLIHLEPEKTISKTIRQYFDRTPQKGWGLRDFVTLKTILEENYLRLNDSLELLYNIRPCSPTETDHALDADESSSEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V5P3</accession>
    <name>A0A6E8V5P3_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Sodium/potassium-transporting ATPase subunit alpha</fullName>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="22">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.</text>
    </comment>
    <comment type="subunit">
        <text evidence="19">The sodium/potassium-transporting ATPase is composed of a catalytic alpha subunit, an auxiliary non-catalytic beta subunit and an additional regulatory subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 20">Cell membrane</location>
            <topology evidence="1 20">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 20">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8V5P3"/>
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        <property type="protein sequence ID" value="ACON002858-PD"/>
        <property type="gene ID" value="ACON002858"/>
    </dbReference>
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        <property type="protein sequence ID" value="ACON002858-PE"/>
        <property type="gene ID" value="ACON002858"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006768"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:P-type sodium:potassium-exchanging transporter activity"/>
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    <dbReference type="GO" id="GO:0030007">
        <property type="term" value="P:intracellular potassium ion homeostasis"/>
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    </dbReference>
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        <property type="term" value="P:proton transmembrane transport"/>
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        <property type="term" value="P:sodium ion export across plasma membrane"/>
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    </dbReference>
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        <property type="entry name" value="P-type_ATPase_IIC"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01106">
        <property type="entry name" value="ATPase-IIC_X-K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43294">
        <property type="entry name" value="SODIUM/POTASSIUM-TRANSPORTING ATPASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SODIUM_POTASSIUM-TRANSPORTING ATPASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00121">
        <property type="entry name" value="NAKATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15 20" id="KW-0406">Ion transport</keyword>
    <keyword evidence="16 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11 20" id="KW-0630">Potassium</keyword>
    <keyword evidence="5 20" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0915">Sodium</keyword>
    <keyword evidence="17" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="7" id="KW-0740">Sodium/potassium transport</keyword>
    <keyword evidence="12" id="KW-1278">Translocase</keyword>
    <keyword evidence="8 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 20" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="73"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="107"/>
            <end position="126"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
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    <feature type="transmembrane region" description="Helical" evidence="20">
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="891"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="929"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="962"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="21">
        <location>
            <begin position="19"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00037422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00038795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU362084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002858-PD"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1000" mass="110861" checksum="F2B098363CBE6A02" modified="2020-08-12" version="1">MPPKKKTDLDDLKQELDIDYHKITPEELYQRLQTHPENGLSHAKAKENLERDGPNALTPPKQTPEWVKFCKNLFGGFALLLWIGAILCFIAYSIQASTVEEPADDNLYLGIVLAAVVIVTGIFSYYQESKSSKIMESFKNMVPQFATVLREGEKLTLRAEDLVIGDVVEVKFGDRIPADIRIIEARNFKVDNSSLTGESEPQSRGPDFTHENPLETKNLAFFSTNAVEGTAKGVVISCGDFTVMGRIAGLASGLDTGETPIAKEIHHFIHLITGVAVFLGVTFFVIAFILGYHWLDAVIFLIGIIVANVPEGLLATVTVCLTLTAKRMASKNCLVKNLEAVETLGSTSTICSDKTGTLTQNRMTVAHMWFDNQIIEADTTEDQSGVQYDRTSPGFKALSRIASLCNRAEFKGGQDGVPILKKEVSGDASEAALLKCMELALGDVLSIRKRNKKVCEIPFNSTNKYQVSIHETEDPSDPRYLLVMKGAPERILERCSTIFINGKEKLMDEEMKEAFNNAYLELGGLGERVLGFCDFMLPSDKYPVGFKFNSDDVNFQVDNLRFVGLMSMIDPPRAAVPDAVAKCRSAGIKVIMVTGDHPITAKAIAKSVGIISEGNETVEDIAQRLNIPVSEVNPREAKAAVVHGSELRDLSSDQLDEILRYHTEIVFARTSPQQKLIIVEGCQRMGAIVAVTGDGVNDSPALKKADIGVAMGIAGSDVSKQAADMILLDDNFASIVTGVEEGRLIFDNLKKSIAYTLTSNIPEISPFLAFILCDIPLPLGTVTILCIDLGTDMVPAISLAYEHAESDIMKRPPRDPFNDKLVNERLISMAYGQIGMIQAAAGFFVYFVIMAENGFLPLKLFGLRKSWDSKAINDLTDSYGQEWTYRDRKTLEFTCHTAFFVSIVVVQWADLIICKTRRNSIFHQGMRNWALNFGLVFETVLAAILSYTPGMDKGLRMYPLKFVWWLPALPFSLSIFVYDEIRRFYLRRNPGGWLEQETYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V5Q1</accession>
    <name>A0A6E8V5Q1_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Cysteine proteinase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the cystatin family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8V5Q1"/>
    <dbReference type="EnsemblMetazoa" id="ACON002879-RC">
        <property type="protein sequence ID" value="ACON002879-PC"/>
        <property type="gene ID" value="ACON002879"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005239"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002879"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030656"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004869">
        <property type="term" value="F:cysteine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00042">
        <property type="entry name" value="CY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02248">
        <property type="entry name" value="Peptidase_C1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000130">
        <property type="entry name" value="Cysteine proteinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.10">
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000010">
        <property type="entry name" value="Cystatin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046350">
        <property type="entry name" value="Cystatin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025661">
        <property type="entry name" value="Pept_asp_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025660">
        <property type="entry name" value="Pept_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039417">
        <property type="entry name" value="Peptidase_C1A_papain-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018073">
        <property type="entry name" value="Prot_inh_cystat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013201">
        <property type="entry name" value="Prot_inhib_I29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08246">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00705">
        <property type="entry name" value="PAPAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00848">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54403">
        <property type="entry name" value="Cystatin/monellin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00287">
        <property type="entry name" value="CYSTATIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00640">
        <property type="entry name" value="THIOL_PROTEASE_ASN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00639">
        <property type="entry name" value="THIOL_PROTEASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026187543" description="Cysteine proteinase" evidence="11">
        <location>
            <begin position="25"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="domain" description="Cathepsin propeptide inhibitor" evidence="13">
        <location>
            <begin position="641"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="12">
        <location>
            <begin position="731"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="24"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="489"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="588"/>
            <end position="622"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002879-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="951" mass="105859" checksum="B2E9CAE617F28894" modified="2020-08-12" version="1">MRPILQLGLLVVVVVAAAATVTRAEEQPNPVTEAEVNSNGPTEEPEEEVQANNLGGRLVGGATPVDVKEPTHIERVRLGLVGYESGKHSNFEILFGTVQVVAGTIHRYKIALKDDDQKVYSTCDVKVFTPLPSAANGSKPDYDFDCQDEEEAAAAGAHRARRDVVAAPPKKLNKVPKAGAAQELTPEEYAKEEHQTRIRTGLQQQSALVDGSGNERKVKVVGATVQLVAGKSYTYRLSFPDDELKRVCKLTVWEKPWLKEKAPQEAFKASFECPDGAKRTKRATVGVDCLGCASPLAANEYGNSEHQERIDKILSFHGLTRGNSLKVINATSQVVAGMKYVYFIQHNNAVCKLTSWERVWLAQSHPEDAYKYTYDCEKEAGPAKVRTRRSVVPGGSRSLSQDELAAAEHLERVDKILVSSGGSKESSNARIVSGTVQIVSGKLYKYAVEFDVDGSSKLCKLSSWERPWLVKKDPTEAYKYTVSCPSEKAAGRQRRHAKKAGSSNELTAEELKDKSHVERIRAGMVSYNSERSKAYNEFEILAGSTQQVAGSLYKYTFRVTSESDIVCKISIWERVWLESQDQRKYNVKCTGDDDTEQQPDIKSPQQPDAPAPTPVTTAPAVKRRSVRSLKIDDDAHVRRMFDKFRHHHRRQYASSMEHEMRFNIFRNNLFKIEQLNKFERGTAKYGVTKFADMTVAEYRAHTGLVVPKHDRANHVGNRVASEEDVAGVGDLPRSFDWRDHGAVTEVKNQGSCGSCWAFSAVGNVEGLHQIKTKKLESYSEQELIDCDKVDNGCGGGYMDDAFKAIEQLGGLELENDYPYEAKAQKSCHFNRSLSHVQVKGAVDMPKNETYIAQYLIKNGPIAIGLNANAMQFYRGGISHPWHPLCNHKSIDHGVLIVGYGIKEYPMFNKTLPYWIIKNSWGPRWGEQGYYRIYRGDNSCGVSEMASSAILA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V5Q4</accession>
    <name>A0A6E8V5Q4_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8V5Q4"/>
    <dbReference type="EnsemblMetazoa" id="ACON002888-RC">
        <property type="protein sequence ID" value="ACON002888-PC"/>
        <property type="gene ID" value="ACON002888"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005269"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002888"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032240"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004989">
        <property type="term" value="F:octopamine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071880">
        <property type="term" value="P:adenylate cyclase-activating adrenergic receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043410">
        <property type="term" value="P:positive regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248">
        <property type="entry name" value="ADRENERGIC RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248:SF66">
        <property type="entry name" value="OCTOPAMINE RECEPTOR BETA-3R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 10" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 10" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 10 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="119"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="151"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="190"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="231"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="279"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="131"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="61"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="70"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002888-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="385" mass="41685" checksum="C72460EDF86AF81C" modified="2020-08-12" version="1">MFTSRSVPPTAQPEVNALLGAATQPITLPGALATVASALASAGSATLTSLAMAPTSGPEVDGAAGGWNGTGDATSGAAGDHQPVGPGSNAVNLTVPTASTIVEPADRLVELTVLCLKSLIFGSIIIGAVLGNALVIISVHKNRKLRVITNYFVVSLAMADMLVALCAMTFNASVELSGRWLFGSFMCDVWNSLDVYFSTASILHLCCISVDRYFAIVRPLEYPLYMTQRTVFFMLANVWVLPALISFTPIFLGWYTTAEHLAALKINPDLCIFVVNKSYAIISSSISFWIPGIVMVTMYYRIYKEAVRQRKALSRTSSNILLNSVQINNASNTLHANHHHHNHHHQHLHHRNHHLRASDCDLGIDIKDIQHDTHSELSDLDVSTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V5Q8</accession>
    <name>A0A6E8V5Q8_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Chloride channel protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the chloride channel (TC 2.A.49) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8V5Q8"/>
    <dbReference type="EnsemblMetazoa" id="ACON002891-RB">
        <property type="protein sequence ID" value="ACON002891-PB"/>
        <property type="gene ID" value="ACON002891"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001039"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002891"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032829"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005247">
        <property type="term" value="F:voltage-gated chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03683">
        <property type="entry name" value="ClC_1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3080.10:FF:000042">
        <property type="entry name" value="Chloride channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.580.10:FF:000026">
        <property type="entry name" value="Chloride channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.580.10:FF:000032">
        <property type="entry name" value="Chloride channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3080.10">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014743">
        <property type="entry name" value="Cl-channel_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050970">
        <property type="entry name" value="Cl_channel_volt-gated"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001807">
        <property type="entry name" value="ClC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45720">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45720:SF10">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00654">
        <property type="entry name" value="Voltage_CLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00762">
        <property type="entry name" value="CLCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81340">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0129">CBS domain</keyword>
    <keyword evidence="8 10" id="KW-0868">Chloride</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="230"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="273"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="378"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="414"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="460"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="534"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="631"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="13">
        <location>
            <begin position="687"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="68"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1019"/>
            <end position="1056"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1086"/>
            <end position="1118"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="747"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="90"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1019"/>
            <end position="1031"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1042"/>
            <end position="1056"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1088"/>
            <end position="1108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002891-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1118" mass="124018" checksum="31F36A48B8079DF4" modified="2020-08-12" version="1">MHIPIKDEYVRDFSFEPEFILRRKDSLDIQREKYHRKRLNSRRKRLKRNALLVLAKTAAIVPPPLGHQHRVVEKNDPGEMPVRPDGVENGSRNSETSACLPTTSSSSSRSSTTNMHTSSRSNAMLIDVPSPDDCPAGSGLDDLDPADKDEIRRSIVDIEIEEFYYMYGRYTKDLGEYAKDEARRLRLLERRRKKDDKARNKELLDVRDNKFFKAASWLWRNTFARLGEDWVFLALLGIIMALLSYVMDKGISMCTNSRVWLYRDLTNHPVLQYLAWVSLPVCLILFSAGFVHLVAPQSIGSGIPEMKTILRGVALKEYLTFKTLVAKVIGLTATLGSGMPLGKEGPFVHIASIVSQLLSKIITSFQSIYENESRNTEMLAAACAVGVGACFAAPIGGVLFSIEVTTTYFAVRNYWRGFFAAVCGATVFRLLAVWFQKADTVTAMFSTNFTSDFPFDPQELFVFALIGRFLYPGIVALIVATLSFPLGFGKYIAGELSTHDQVHQLFSNFTWTKHELTVEQAAVVSNWRTPETNVFANLIIYLLYTFFMSIIASTIPVPSGMFIPVFKIGAAFGRIIGEAMHLWFPHGVRYGGMLAPIIPGGYSVVGAAAFSGAVTHTVSVGVIVFEMTGQITHIVPVMIAALISNAVAALLQPSMYDSIILIKKLPYLPDLLPSGSGMYNIYVEDFMVRDVRYIWKGISYQQLKEILKANKSLRSLPIVDSPDNRTLLGSVQRYELIKMIDKHIGREKRLEVAAKLMKEAEEKAREEQERQKREAAEAAKARRPSRFEVVPAPDILKLRERANNEMLPPQARRESNASLTSGPVFGATQPKKSILKKTNSFTLKGFSPLSPHSPVHTPYTTITGTESRIRSAFDIIFRKSATLQDVTPDPEIGSIGTPSIVGSEVAPLTPGISKKVQLPRERVIDMSPEDQKAWEMEEMAKPIDVGNLHIDPAPFQLVERTSILKVHSLFSMVGINHAYVTNVGRLVGVVALKELRTAIESVNNGTLTPETVKAKQEEEARALAQKGKESDNNNGISAEDPLLPKRPGDTFSDKKVNNTITSMDSALSNSDNCSDIELDNINIDHGDTAATTNGTITTTTTTTPSTKHTNNHSGSQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V5R6</accession>
    <name>A0A6E8V5R6_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="6">U2 snRNP auxiliary factor large subunit</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8V5R6"/>
    <dbReference type="EnsemblMetazoa" id="ACON002908-RB">
        <property type="protein sequence ID" value="ACON002908-PB"/>
        <property type="gene ID" value="ACON002908"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000665"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002908"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031915"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12231">
        <property type="entry name" value="RRM2_U2AF65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12232">
        <property type="entry name" value="RRM3_U2AF65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000097">
        <property type="entry name" value="U2 snRNP auxiliary factor large subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23139">
        <property type="entry name" value="RNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="57"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="152"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002908-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="250" mass="27886" checksum="7946423A4899959B" modified="2020-08-12" version="1">MCSFSLFRFYPRSLQYSSFPSFTCYLTPTVSHSMPNSNENTPRYDALLLYDRSRNCEQVKELLLSFGQLKAFNLVKDAATGLGKGYAFAEYVEYTVTDQAIAGLNGMQLGDKKLIVQRASVGAKNSNAAVVAPVQIQVPGLSLVGSSGPPTEVLCLLNMVTPDELKDEEEYEDILEDIREECNKYGVVRSVEIPRPIEGVDVPGCGKVFVEFNSIVDCQKAQQALTGRKFSDRVVVTSYFDPDKYHRREF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V5T6</accession>
    <name>A0A6E8V5T6_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidyl-prolyl cis-trans isomerase</fullName>
            <shortName evidence="4">PPIase</shortName>
            <ecNumber evidence="4">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 4">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cyclophilin-type PPIase family.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8V5T6"/>
    <dbReference type="EnsemblMetazoa" id="ACON002952-RB">
        <property type="protein sequence ID" value="ACON002952-PB"/>
        <property type="gene ID" value="ACON002952"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004993"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002952"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042269"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016018">
        <property type="term" value="F:cyclosporin A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000025">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase CYP19-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071:SF565">
        <property type="entry name" value="MOCA-CYP, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="5">
        <location>
            <begin position="25"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002952-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="158" mass="17169" checksum="543D0C73D1F7DF7D" modified="2020-08-12" version="1">MTIDSDAGGAAAEPPPPQQEKIRCFFDVSLGGLPAGRIVFELFPAVAPKTCENFRALCTGEKGIGQKTGKPLHYKGIIFHRVVKDFMIQSGDFSNGNGTGGESIYGGTFDDEEFTLKHDRAFLLSMANRGKNTNGSQFFIGHTLLPCQRFTAEQPQLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V634</accession>
    <name>A0A6E8V634_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Munc13-4</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Late endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Recycling endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the unc-13 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040225680.2">
        <property type="nucleotide sequence ID" value="XM_040369746.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8V634"/>
    <dbReference type="EnsemblMetazoa" id="ACON003164-RB">
        <property type="protein sequence ID" value="ACON003164-PB"/>
        <property type="gene ID" value="ACON003164"/>
    </dbReference>
    <dbReference type="GeneID" id="120951188"/>
    <dbReference type="CTD" id="6769"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011640"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003164"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041615"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055037">
        <property type="term" value="C:recycling endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099503">
        <property type="term" value="C:secretory vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04009">
        <property type="entry name" value="C2B_Munc13-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010439">
        <property type="entry name" value="MUN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014770">
        <property type="entry name" value="Munc13_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014772">
        <property type="entry name" value="Munc13_dom-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052095">
        <property type="entry name" value="UNC-13_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45999:SF2">
        <property type="entry name" value="PROTEIN UNC-13 HOMOLOG 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45999">
        <property type="entry name" value="UNC-13-4A, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06292">
        <property type="entry name" value="MUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00360">
        <property type="entry name" value="C2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51258">
        <property type="entry name" value="MHD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51259">
        <property type="entry name" value="MHD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0967">Endosome</keyword>
    <keyword evidence="5" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="9">
        <location>
            <begin position="143"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="MHD1" evidence="10">
        <location>
            <begin position="622"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="domain" description="MHD2" evidence="11">
        <location>
            <begin position="857"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="9">
        <location>
            <begin position="974"/>
            <end position="1112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="39"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="39"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003164-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1151" mass="132127" checksum="5E79C7B8B8386BEB" modified="2020-08-12" version="1">MSTYSLPRLLHGGGLSGNKKFNIKFGTLRLTKSFRSLDRREKPAVQRQQERDYGSTIARRASENPYGTTTANRPGRLPYHPFRGVRKEEIEQQASLIVHSMTIEELYEEILFENIHNIGCDDEELNVDVLFPYIQEAFKMSDEKHNEILQIARDKEAPQLRLNVEIVEAKDLEPKDSNGLSDPFVTMYIASNPNHRYNTSVKAGTLNPVWEEHFSLPIAEDANDANLIVEVWDFDPAETVKEKMNKLFEVKGVRGLRKLMKEIAQTASTGKHDNELIGRTSIPLKSIPASGMLMWYNLDKKNKLRRQGTIRIRLNFSSEKNSQVAAQEHRHLLRILLLHELESSKVTPYWWSGKFTIQGEAVLTQHSAQSGLSSTTEAFIQWSVFAAIHQDHPLSFVLFDTLLEKLVRPIQTLAVSEEEQKSFWEATKKLLPSCFSAVRKLRKKNTGDKLLLKTLNSVLNIIAKVALLEPPEGTDLFPVQMYGWIRRTNETEPNWDIREALASAVVSGAEDWFGGIKEGHFLQTGTDEDRLQNLIKIIQLVRSDIQKSIEYYDKTFQEIMHFPYTKELYITYELKLAELIKPTVEDICRKLKRIDLPETSSHRGGDSGFIEYEDINMGTTLFELYLVLKRFCTLGTALSPGESNFAIDEYHRWFTTGVTHWLDIAVYKALMRIHKAIELDKLQPVDETVKYSSSAVDTLAIFYQIKIFWQQLAWPDVEGAYIFVAKIVDDICRCCVFYADRMSARVENLGVVENVYEKKFEVTSEWCLAINNIDYIRQSLKPFATELGVDDILAKLGDVQSSIEADRCKETLRAVLDNAIDTEKNKILDLVEKLARKMAPAMRRFLVEGAELLQQDSNSLDRLMMYMEESLSVLNAELNEINFERVLDAIWAELTTILYDLIQSNLDKRRPPSFFANLRDTLHLMVANFKTAENRESETAADKETLAHIERLLQLHGYETTDLIHQYYLDRLEEQNQSDASATGYGMLTVQCFFKENVLELEIVNARNLKPMDGNGSCDPFVRVHFLPEERFVGVAKPKTQCQSKTLFPLYDEKFVITFTPEQRAIPNALIMFSIKDKDLFGMSNQYLAECYLSFNDIADISGDAGKIEQKHLVLTRPQKMDIDCLRALEYRQGDKQAKEFIKKLKQKMGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V656</accession>
    <name>A0A6E8V656_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="5">RRM domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8V656"/>
    <dbReference type="EnsemblMetazoa" id="ACOM035693-RA">
        <property type="protein sequence ID" value="ACOM035693-PA"/>
        <property type="gene ID" value="ACOM035693"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON003219-RA">
        <property type="protein sequence ID" value="ACON003219-PA"/>
        <property type="gene ID" value="ACON003219"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004549"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003219"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038297"/>
    <dbReference type="Proteomes" id="UP000075882">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="entry name" value="Eukaryotic translation initiation factor 4H"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4B/4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236:SF11">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
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    </dbReference>
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        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RNA-binding domain, RBD"/>
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    </dbReference>
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        <property type="entry name" value="RRM"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="26"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="103"/>
            <end position="346"/>
        </location>
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        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
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        <location>
            <begin position="103"/>
            <end position="112"/>
        </location>
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            <begin position="120"/>
            <end position="143"/>
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        <location>
            <begin position="155"/>
            <end position="169"/>
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        <location>
            <begin position="182"/>
            <end position="206"/>
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        <location>
            <begin position="215"/>
            <end position="227"/>
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        <location>
            <begin position="237"/>
            <end position="247"/>
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        <location>
            <begin position="296"/>
            <end position="309"/>
        </location>
    </feature>
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        <location>
            <begin position="322"/>
            <end position="335"/>
        </location>
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            <begin position="336"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003219-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="346" mass="36128" checksum="E6131C68C51F4F76" modified="2020-08-12" version="1">MAGRGTHDNNRYGDRARKPLPTEPPYLAYVGNLPNGVVQGDINAIFKEYAVKSVRLVKDKETDVFKGFCYVEFDTLEDLEKVLQLDGMIVLNDRPEPLRIDVAEQKKNDRGGFNRRGGQQNRGGPGGPGNGGGGGGGGGGFNRGGPNQMNNRPIGSGGGGGGVGGGGSGDRAPNRGRYGNFGDERDGGGRDEWSRDNDRDGGRDGRGGAGGGGRYNDRDNYGRDDGNRYGNYGRNNQYRDNDRRKDAAPSNYPPPSNPNLNMEERPRLKLAPRSTNAPLNALAETKQSAAIFGNARPREEKLGGPETGRKHANSVGSEGSSADGGGSGGGGGGGNSASSSATGGDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V855</accession>
    <name>A0A6E8V855_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="10">One cut domain family member</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CUT homeobox family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8V855"/>
    <dbReference type="EnsemblMetazoa" id="ACON000061-RB">
        <property type="protein sequence ID" value="ACON000061-PB"/>
        <property type="gene ID" value="ACON000061"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006214"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000061"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038917"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.260.40:FF:000005">
        <property type="entry name" value="One cut domain family member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003350">
        <property type="entry name" value="CUT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051649">
        <property type="entry name" value="CUT_Homeobox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14057:SF47">
        <property type="entry name" value="HOMEOBOX PROTEIN ONECUT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14057">
        <property type="entry name" value="TRANSCRIPTION FACTOR ONECUT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02376">
        <property type="entry name" value="CUT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01109">
        <property type="entry name" value="CUT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51042">
        <property type="entry name" value="CUT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0371">Homeobox</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="CUT" evidence="9">
        <location>
            <begin position="460"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="201"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="343"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="13"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="43"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="216"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="238"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="248"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="283"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="353"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000061-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="583" mass="63285" checksum="2613F9EF069D3ACC" modified="2020-08-12" version="1">MEQCNNDRPCVISSGNFFPQNSKSSAKQQQQQQHTQAISKHLTQKDDPHLHSPHHQDQSSLGEIEVILVQQHKQDALSVIIHPQESMQQINMCLGSQNPGSVDSMMSSDFQTLSPMHDRDSPVSLNTNTSYATLTPLQPLPPISTMSDKFAYSIGGNICEAFPGINSGAVGVNIEINSCYNLEKLGVIHSPVHLSAVQELSPDGGDVVHSHHHQQQHQQQQQQQQQQHQQHQQQQQHQQHHHHHHHHPDVLQEKGHGLKEHGLKEHLAGGPDKDPTLQDADIKGGGGGGGGGGAGTGDPCILVRGSPGLDDRFLGSGKELVVRHHPKDDGEQFSGTVQYLEHPNGSDVVLHGTGNGTGPGGGDSSGGGGGGGGGSSGGILTGKEHHQSVFLNKSFSSKTNISDLNLCKEIVIDDVYEESELDHVKLEEKKLEESCNQPLTNGGGAAAGKSGSCVSLGDVGCLNGDLEEINTKELAHRISSELKRYSIPQAIFAQRVLCRSQGTLSDLLRNPKPWSKLKSGRETFRRMYKWIQEPEYQRMSALRLAATQSAKPGYCSFSLFPLLFRCTIPSDAALKSKKKNRKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V863</accession>
    <name>A0A6E8V863_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="7">BTB domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8V863"/>
    <dbReference type="EnsemblMetazoa" id="ACON000080-RB">
        <property type="protein sequence ID" value="ACON000080-PB"/>
        <property type="gene ID" value="ACON000080"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_003472"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000080"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032448"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048468">
        <property type="term" value="P:cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003006">
        <property type="term" value="P:developmental process involved in reproduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18315">
        <property type="entry name" value="BTB_POZ_BAB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000138">
        <property type="entry name" value="Fruitless, isoform N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051095">
        <property type="entry name" value="Dros_DevTransReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110">
        <property type="entry name" value="BTB DOMAIN TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110:SF107">
        <property type="entry name" value="SEX DETERMINATION PROTEIN FRUITLESS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="5">
        <location>
            <begin position="78"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="6">
        <location>
            <begin position="524"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="160"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="348"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="456"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="652"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="722"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="165"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="194"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="230"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="246"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="281"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="292"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="357"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="380"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="465"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="722"/>
            <end position="746"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000080-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="759" mass="79638" checksum="7B157516E1A47204" modified="2020-08-12" version="1">MASSPALPLYASRYPTPNGYPQINGEVDAPLDFRKVESLRRNSTDTGIMDQQYCLRWNNHQSNLTTVLTTLLQDEKLCDVTLACEKGMVKAHQAILSACSPYFEQIFVENKHPHPIIYLRDVEVNEMRALLDFMYQGEVNVGQHNLQNFLKTAESLKVRGLTESSADRYSADTDSKLRSERIRDSRDERDSLPNASSNNSNNNNNSSSNNNNNNNTISSNNNNNSLHHGPLRDKELTEHEQLERLQQQQQQTHHQQQQHPSSHQQQSQQHPSSQHQQPTHQTHHHHHHHQHGGGVGGGGGGGGGGGGGGGSAGPVQQPSRSASIDLMQSALVDERDYLAAEDREISTVENKKKRKMSTTCDNSSPSTPSLMNERQGGYESQASSHSSFKQSPKPEDEFKVSSPAPVHPLAGHPLSGIKQELPELPVRHSLSSELMQPIPINMNADDMNNILAPGNMGSLNESGDSDAHLSHPDHPDNIDGSKAWHMRLTFERLSGGCNLHRCKLCGKVVTHIRNHYHVHFPGRFECPLCRATYTRSDNLRTHCKFKHPMFNPDTRKFENMLSPTMASQAAAAAAAISAAAAAANSSFKPDFSTAAAVANSFKSEQFSSAAAAVANYALGTFKSDFPPIPNSSAAAAAAAVAAAVAASVSPGSGSGGGGGGGGGGSVGSGGIGSSSLGGGGGSGRSSSGGGMIGMHSVAAGAAVAAGGGVAGMMSTGAGVNRGGDGGCGSIGGEVGSVGGGGGGGGSSVRDGNNGGELSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V871</accession>
    <name>A0A6E8V871_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Signal transducer and activator of transcription</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">Ngousso</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="12">Forms a homodimer or a heterodimer with a related family member.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the transcription factor STAT family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040238027.1">
        <property type="nucleotide sequence ID" value="XM_040382093.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8V871"/>
    <dbReference type="EnsemblMetazoa" id="ACON000099-RC">
        <property type="protein sequence ID" value="ACON000099-PC"/>
        <property type="gene ID" value="ACON000099"/>
    </dbReference>
    <dbReference type="GeneID" id="120958964"/>
    <dbReference type="CTD" id="42428"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_003675"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000099"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034609"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd09919">
        <property type="entry name" value="SH2_STAT_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16855">
        <property type="entry name" value="STAT5_CCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.532.10:FF:000005">
        <property type="entry name" value="Signal transducer and activator of transcription"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000029">
        <property type="entry name" value="Signal transducer and transcription activator 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.630:FF:000003">
        <property type="entry name" value="Signal transducer and transcription activator 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000048">
        <property type="entry name" value="Signal transducer and transcription activator 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.20">
        <property type="entry name" value="STAT transcription factor, all-alpha domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.630">
        <property type="entry name" value="STAT transcription factor, DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.532.10">
        <property type="entry name" value="STAT transcription factor, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001217">
        <property type="entry name" value="STAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046994">
        <property type="entry name" value="STAT5_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048988">
        <property type="entry name" value="STAT_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036535">
        <property type="entry name" value="STAT_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013800">
        <property type="entry name" value="STAT_TF_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015988">
        <property type="entry name" value="STAT_TF_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013801">
        <property type="entry name" value="STAT_TF_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012345">
        <property type="entry name" value="STAT_TF_DNA-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013799">
        <property type="entry name" value="STAT_TF_prot_interaction"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11801">
        <property type="entry name" value="SIGNAL TRANSDUCER AND ACTIVATOR OF TRANSCRIPTION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01017">
        <property type="entry name" value="STAT_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02864">
        <property type="entry name" value="STAT_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02865">
        <property type="entry name" value="STAT_int"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21354">
        <property type="entry name" value="STAT_linker"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Transcription factor STAT-4 N-domain"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-0010">Activator</keyword>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 14" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13 14" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="10 14" id="KW-0804">Transcription</keyword>
    <keyword evidence="7 14" id="KW-0805">Transcription regulation</keyword>
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        <location>
            <begin position="578"/>
            <end position="677"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="696"/>
            <end position="736"/>
        </location>
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    <feature type="coiled-coil region" evidence="15">
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            <begin position="143"/>
            <end position="184"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="698"/>
            <end position="736"/>
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            <dbReference type="ARBA" id="ARBA00004496"/>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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            <dbReference type="PROSITE" id="PS50001"/>
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        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000099-PC"/>
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    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
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    <sequence length="736" mass="84652" checksum="09DAC7C50BB602CE" modified="2020-08-12" version="1">MSLWARVNQLPQPILEQIRFIYGSNFPIEVRHYLADWIEERLLNAPVYTNDQEAVYEQDAANFLNQLIMELERTAINLPESNFTIKIRLNESARNFRQLFSHNPAQLYQHLMNCLHRERQCVAYPDECVNVQDPEVTEVFNAVQQLQIMVRTNENDNRNLMKEYEHLLLEVHELQKNRAQLETIENAEMRAHAHNQLAQHQKMVNDRLQLCTGKRLALVDGFRKTILITDEVQNKVLNKYLSQWKINQGFAGNGASMMSASNLDTIQAWCESLAEIIWSTKDQIRLAIKNKSKLHVEQEDVPDLLPQAMVDVTNLLKMLITNTFIIEKQPPQVMKTNTRFAATVRLLVGNTLNIKMVNPQVKVSIISEAQAQQTQQTNKASEQSCGEIMNNIGNLEYNETTKQLSVSFRNMQLKKIKRAEKKGTECVMDEKFALLFQSSFAVGHGDLVFSVWTISLPVVVIVHGNQEPQSWATITWDNAFADINRIPFQVPDKVIWNQLAEALNMKFRASTGRSLTAENMHFLCEKAFKTNLPFPVPNDLTIMWSQFCKEPIPDRSFTFWDWFYAAMKVTREHLRGPWMDGSIIGFIHKSKAEDYLLKCPRGTFLLRFSDSELGGITIAWVNEGNDGQPQILHIQPFTAKDFSTRSLSDRIRDFDDLFYLYPNKPKHEAFDRYTTPAGPPRNKNYIASEVRAVLMPGPTNNQMNSFPNTPSYNIQSPDASRDTPSTGYQNSTIMHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V879</accession>
    <name>A0A6E8V879_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Uridine phosphorylase</fullName>
            <ecNumber evidence="5">2.4.2.3</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the reversible phosphorylytic cleavage of uridine to uracil and ribose-1-phosphate which can then be utilized as carbon and energy sources or in the rescue of pyrimidine bases for nucleotide synthesis. Shows broad substrate specificity and can also accept deoxyuridine and other analogous compounds.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>uridine + phosphate = alpha-D-ribose 1-phosphate + uracil</text>
            <dbReference type="Rhea" id="RHEA:24388"/>
            <dbReference type="ChEBI" id="CHEBI:16704"/>
            <dbReference type="ChEBI" id="CHEBI:17568"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57720"/>
            <dbReference type="EC" id="2.4.2.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Pyrimidine metabolism; UMP biosynthesis via salvage pathway; uracil from uridine (phosphorylase route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the PNP/UDP phosphorylase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.3" evidence="5"/>
    <dbReference type="RefSeq" id="XP_040226426.1">
        <property type="nucleotide sequence ID" value="XM_040370492.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040226436.1">
        <property type="nucleotide sequence ID" value="XM_040370502.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040226444.1">
        <property type="nucleotide sequence ID" value="XM_040370510.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040226452.1">
        <property type="nucleotide sequence ID" value="XM_040370518.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040226472.1">
        <property type="nucleotide sequence ID" value="XM_040370538.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040226481.1">
        <property type="nucleotide sequence ID" value="XM_040370547.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040226489.1">
        <property type="nucleotide sequence ID" value="XM_040370555.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8V879"/>
    <dbReference type="EnsemblMetazoa" id="ACON000188-RB">
        <property type="protein sequence ID" value="ACON000188-PB"/>
        <property type="gene ID" value="ACON000188"/>
    </dbReference>
    <dbReference type="GeneID" id="120951657"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010602"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000188"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034586"/>
    <dbReference type="UniPathway" id="UPA00574">
        <property type="reaction ID" value="UER00633"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004850">
        <property type="term" value="F:uridine phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009166">
        <property type="term" value="P:nucleotide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044206">
        <property type="term" value="P:UMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006218">
        <property type="term" value="P:uridine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17763">
        <property type="entry name" value="UP_hUPP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Nucleoside phosphorylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Nucleoside_phosphorylase_CS"/>
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        <property type="entry name" value="Nucleoside_phosphorylase_d"/>
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        <property type="entry name" value="Nucleoside_phosphorylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010059">
        <property type="entry name" value="Uridine_phosphorylase_euk"/>
    </dbReference>
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        <property type="entry name" value="euk_UDPppase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43691:SF11">
        <property type="entry name" value="FI09636P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43691">
        <property type="entry name" value="URIDINE PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01048">
        <property type="entry name" value="PNP_UDP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53167">
        <property type="entry name" value="Purine and uridine phosphorylases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01232">
        <property type="entry name" value="PNP_UDP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleoside phosphorylase" evidence="6">
        <location>
            <begin position="58"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="143"/>
            <end position="146"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="222"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR610059-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000188-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="345" mass="38836" checksum="2732C4FBCC65FB77" modified="2020-08-12" version="1">MSWIYRSSVSEDEERDEYNDGSVKLRNPNIELMDQDILYHLALGSGSHDLHEMFGDVKFVCMGGTPKRMETFAHFIMDEIGYKLPAGTQLQDISAFSYRYSMYKVGPVLSISHGMGVPSVGILLHELIKLMYHAKVKDPIFMRIGTCGGIGVEGGTVVITEDAYDGLLRNSYELAILGKIVHRPAILDKRLVRELKSLATQDDPYDTISGKTMCTYDFYEGQGRLDGAFCEFTETDKMAYLEKLRDFGVVNIEMECTIFAALTHYAGIKAAIVCVTLLDRLKGDQVMAPKEVMNEWQQRPQILVSRLIKKHLAQMGQLKSLASTPSSIKSPRRFKLVQQESEAHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V9X3</accession>
    <name>A0A6E8V9X3_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="4">FoP_duplication domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8V9X3"/>
    <dbReference type="EnsemblMetazoa" id="ACON001438-RA">
        <property type="protein sequence ID" value="ACON001438-PA"/>
        <property type="gene ID" value="ACON001438"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006573"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001438"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035534"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025715">
        <property type="entry name" value="FoP_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13865">
        <property type="entry name" value="FoP_duplication"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01218">
        <property type="entry name" value="FoP_duplication"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Chromatin target of PRMT1 protein C-terminal" evidence="3">
        <location>
            <begin position="216"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="127"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="24"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="130"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="141"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="160"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="208"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="255"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001438-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="304" mass="32226" checksum="7383554B8006E33A" modified="2020-08-12" version="1">SRGRSMSRGRSASRTRSNSRGRQPSLTRSNSQTNLRRAGSRTNLSRAGSRTNLSRAGSRNALNVVNRKPIAPNDARRRINRNNLLANRLGTVNNAATAITRGRSRSRSRVRGGAPAVAANVPAVAANGRARSRSRKRAGSRARAGSVNSRLGMNRTNEPAATAGGRANGGRVTKRNRNGPRVNASIVGTRAGRPQKREAPKRKGAGGKAAKAAAARSNGAGANGAARGRGAAAAATAAAPGRRGRSRSRRDNSRVRGRSASKNRTAHVKQQPKSREELDSELDQYMANTKSSLDKEMDQYMNGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V9X4</accession>
    <name>A0A6E8V9X4_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">UTP--glucose-1-phosphate uridylyltransferase</fullName>
            <ecNumber evidence="3 9">2.7.7.9</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">UTP--glucose-1-phosphate uridylyltransferase catalyzing the conversion of glucose-1-phosphate into UDP-glucose, a crucial precursor for the production of glycogen.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>alpha-D-glucose 1-phosphate + UTP + H(+) = UDP-alpha-D-glucose + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19889"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.7.7.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:19890"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homooctamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the UDPGP type 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.9" evidence="3 9"/>
    <dbReference type="RefSeq" id="XP_040226373.1">
        <property type="nucleotide sequence ID" value="XM_040370439.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8V9X4"/>
    <dbReference type="EnsemblMetazoa" id="ACON001257-RB">
        <property type="protein sequence ID" value="ACON001257-PB"/>
        <property type="gene ID" value="ACON001257"/>
    </dbReference>
    <dbReference type="GeneID" id="120951633"/>
    <dbReference type="CTD" id="39065"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011082"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001257"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034883"/>
    <dbReference type="UniPathway" id="UPA00164"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003983">
        <property type="term" value="F:UTP:glucose-1-phosphate uridylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006011">
        <property type="term" value="P:UDP-alpha-D-glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00897">
        <property type="entry name" value="UGPase_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.10.10:FF:000001">
        <property type="entry name" value="UTP--glucose-1-phosphate uridylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000002">
        <property type="entry name" value="UTP--glucose-1-phosphate uridylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002618">
        <property type="entry name" value="UDPGP_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016267">
        <property type="entry name" value="UDPGP_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43511">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01704">
        <property type="entry name" value="UDPGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000806">
        <property type="entry name" value="UDPGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="11">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="222"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="253"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="398"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR000806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000806-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000806-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001257-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="511" mass="58175" checksum="13BE26F7D7523AE8" modified="2020-08-12" version="1">MLNIPNEKVRGHQRIPSDTKEFHEATKRDALMRLKKDLDHLLETVEESRKTGVQKEMCGFEALYHRFLQEDGPSVEWDRIEKLPEEAVKDYSTLKLPQEAHIRDMLDKLVVVKLNGGLGTSMGCHGPKSVIPVRNDLTFLDMTVQQIEFLNKKYNANVPLVLMNSFNTDVDTEKVIRKYKGFQVQIYTFNQSCYPRISRDSLLPIAKDFDIENDIEAWYPPGHGDFYQSFQNSGLLRKFLEEGREYCFLSNIDNLGATVDIKILNRLIGDDRQGDKPIEFVMEVTDKTRADVKGGTLIHYENKLRLLEIAQVPKEHVDDFKSVKTFKFFNTNNIWARLESIERVLNAKTMNMEIIVNNKTLDNGIRVIQLETAVGAAMKCFDGGIGINVPRSRFLPVKKTSDLLLVMSNLYSLKYGSLVMSPQRMFPTTPLVKLGDNHFSKVKEFLSRFANIPDLIELDHLTVSGDVTFGRGVSLRGTVIIIANHGDRIDIPAGAILENKIVSGNMRILDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V9X7</accession>
    <name>A0A6E8V9X7_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">CBS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="4">AMPK is a heterotrimer of an alpha catalytic subunit (PRKAA1 or PRKAA2), a beta (PRKAB1 or PRKAB2) and a gamma non-catalytic subunits (PRKAG1, PRKAG2 or PRKAG3). Interacts with FNIP1 and FNIP2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the 5'-AMP-activated protein kinase gamma subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8V9X7"/>
    <dbReference type="EnsemblMetazoa" id="ACON001446-RA">
        <property type="protein sequence ID" value="ACON001446-PA"/>
        <property type="gene ID" value="ACON001446"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002448"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001446"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040820"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031588">
        <property type="term" value="C:nucleotide-activated protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019887">
        <property type="term" value="F:protein kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04618">
        <property type="entry name" value="CBS_euAMPK_gamma-like_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04641">
        <property type="entry name" value="CBS_euAMPK_gamma-like_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050511">
        <property type="entry name" value="AMPK_gamma/SDS23_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780">
        <property type="entry name" value="AMP-ACTIVATED PROTEIN KINASE, GAMMA REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780:SF35">
        <property type="entry name" value="LD22662P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0129">CBS domain</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CBS" evidence="7">
        <location>
            <begin position="124"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="7">
        <location>
            <begin position="205"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="7">
        <location>
            <begin position="278"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="7">
        <location>
            <begin position="352"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="411"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="415"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="427"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="450"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="495"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="524"/>
            <end position="538"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
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    <evidence type="ECO:0000256" key="6">
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            <dbReference type="PROSITE" id="PS51371"/>
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            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="562" mass="63825" checksum="F5F8C0FB9F9F5901" modified="2020-08-12" version="1">MYHVKGAHLPLLNITCMCYVNPNFKKISNPKKRSNYGCRRRWFAGREPWPTLHFYSNYAGRLVLGAGFHDFFNTETYPLESIKEFEAISSAWKRHLAELLKKEEDESQIFVKFFRFHKCYDLVPTSAKLVVFDTQLLVKKAFYALVYNGVRAAPLWDSKRQEFVGMLTITDFIKILKMYYKSPNASMDELEEHKLDTWRKVLQEDVKKLVSIGPDASLYDAIKMLVHNRIHRLPVIDPVTGNVLYILTHKRILRFLFLYINELPKPSYMQKTLREVRIGSYNNIETATEDTSIITALHKFVDRRVSALPMVDSEGRLTDIYAKFDVINLAAEKTYNDLDVSLKKANEHRNAWFEGVHHCTLDETLYTIMERIVRVEVHRLVVVDELKKVIGIISLSDILLYLVLRPSGDGIGDSESLRATDPRLMKAGSPAGGSSSSKLQATKRDSSNESIEEEKETTEEDAEQEQQDAAAHKTETTITPATPSNDEDGQKNEAEVEVETADDAVPELAVEELAEEAKEDPDDTASTPVDRSSANDSPVAQEMLATDVPLQSVQREVGLVSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V9Y1</accession>
    <name>A0A6E8V9Y1_ANOCL</name>
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        <submittedName>
            <fullName evidence="12">Mixed-lineage leukemia protein</fullName>
        </submittedName>
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        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON001451-RB">
        <property type="protein sequence ID" value="ACON001451-PB"/>
        <property type="gene ID" value="ACON001451"/>
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    <dbReference type="VEuPathDB" id="VectorBase:ACON001451"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040927"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="CC_AF10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15672">
        <property type="entry name" value="ePHD_AF10_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PHD_AF10_AF17"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="protein AF-10 isoform X1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="protein AF-10 isoform X2"/>
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        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
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        <property type="entry name" value="AF10-like_CC"/>
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        <property type="entry name" value="EPHD"/>
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    <dbReference type="InterPro" id="IPR050701">
        <property type="entry name" value="Histone_Mod_Regulator"/>
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    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
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        <property type="entry name" value="Znf_FYVE_PHD"/>
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    <dbReference type="InterPro" id="IPR001965">
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        <property type="entry name" value="Znf_PHD-finger"/>
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    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
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        <property type="entry name" value="ALHAMBRA, ISOFORM P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13793">
        <property type="entry name" value="PHD FINGER PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13831">
        <property type="entry name" value="PHD_2"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF13832">
        <property type="entry name" value="zf-HC5HC2H_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
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    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 8" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="5"/>
            <end position="57"/>
        </location>
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            <end position="181"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="9">
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            <begin position="188"/>
            <end position="592"/>
        </location>
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            <begin position="1025"/>
            <end position="1061"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
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            <begin position="231"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
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            <begin position="252"/>
            <end position="317"/>
        </location>
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            <end position="331"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="9">
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            <end position="354"/>
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            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="391"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="405"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
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            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="465"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="492"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
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            <begin position="502"/>
            <end position="514"/>
        </location>
    </feature>
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            <end position="538"/>
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    <feature type="compositionally biased region" description="Gly residues" evidence="9">
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        </location>
    </feature>
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        </location>
    </feature>
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        </location>
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        </location>
    </feature>
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            <end position="806"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
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            <end position="833"/>
        </location>
    </feature>
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            <end position="877"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="888"/>
            <end position="908"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001451-PB"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1329" mass="129389" checksum="C41CDE10BA87FFDE" modified="2020-08-12" version="1">MKEMVGGCCVCSDDRGWSENPLVYCDGQSCAVAVHQACYGIVTVPSGPWYCRKCESQERPARVRCELCPSRDGALKRTDNQGWAHVVCALYIPEVRFGNVTTMEPIILQLIPQERYNKTCYICQDMGKGSRANVGACMQCNKSGCKQQFHVTCAQQLGLLCEEAGNYLDNVKYCGYCQHHYSKLKKGGNVKTIPPYKPISHEANSSDGPSSPEKEMEPPPPQQHSSSSAAGSGGTGGGGGGGGGGGSGAGGSSSSSSGLKSSSRLSGEPSVGGSSSTSSSSSSSSSKQRKSSSASKSSSGSGSGASLSSSSSSSVLASGGGGGSGGSGASGMSGNSSMSTSSSSSSSSGVSSMSGSGGSGSVSNSSGSGIGGSGIAGSVGGSGSSKTSSGSSGGTGGGGSSGSSSKEKDKYSKSRDKSSKSSKSSSSSSTSGGSGSNFNNSTSTSSSAGGSSNQSKDVDMGGTAGSSSGSMGALGANASSQSSSQSGYSSTAGGGGSGPGGSSSSSSGSSSNSSSKHHSDKPDKGSGGGGSSSQGGGPLSTNSGVGSGRAASLSPAAIPTTLIIKPPQDHTGGSGKEPLSKEAIAKMSTSSNFTETIVVNSESVYNHAGSGSGNAGNTSVIESGKLAMGGSGAGSGGSGGSYGGSTGPTGGSSTGSSSSSSSGGKKRKADARSTPTSSPTPHSPQQPASSQHTPSLVVSVPLSTATVPGVNLPASNNSNSSSHTGSSSSSSSSSSNVNHSASSNSNSTPNIVSPGHHQGSDRGGGGGSHINNSNSNSGGNSNLYQQISHRAGDQLMNASANRSSPVIQQQSTAQNIIQSSSSSSTAGSSSSSTVGPSIGHHHLERQSPSMRSSPAGVTTGGANVITSLHHSQSQQPDLLSPAGGGATVLQSVSPVPMSTIQRTSSPSTLHAGDNSSSSGGGGGGLKFGYEKQAPTTNARIAALQEEEASTGRRSRYGTPEMIVSSGGVVPYSMSSTMTAASSSSYGGSSGGGGGGLKFSYEAQPTNPLSAVSTASMMGSSGSVIAAPQVKDSPPSSPGSDAGGSAAGTAIVSGRGTKRNRKMSSNAAAVNSGAGAVPTTSVAGQTGGPSIVPASIVAGGSADAKDGKLFQNGGSSSAAAGGSVVSATHMLGNQLNPSSSVAQKMSDQLSMEIEAHAYVPGPIDAVPTLMGPQFPGKNRTNNSQSMPVGAVGGGNSLSSMLTGGATATANGNTPQSLEQLLERQWEQGSQFLMEQAQHFDIASLLSCLHQLRSENIRLEEHVNNLVARRDHLLAVNARLAIPLNPTAALGGVGMIGGSGGSGPGAGGAATGGAGVGGAGIGSLPGMLTWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V9Y2</accession>
    <name>A0A6E8V9Y2_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein-serine/threonine phosphatase</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
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            <taxon>Pterygota</taxon>
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            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="EnsemblMetazoa" id="ACON001455-RA">
        <property type="protein sequence ID" value="ACON001455-PA"/>
        <property type="gene ID" value="ACON001455"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011934"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001455"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039614"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05162">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000313">
        <property type="entry name" value="PWWP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14312:SF1">
        <property type="entry name" value="BASIC-LEUCINE ZIPPER TRANSCRIPTION FACTOR A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14312">
        <property type="entry name" value="CREB/ATF BZIP TRANSCRIPTION FACTOR"/>
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            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
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                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
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        </source>
    </evidence>
    <sequence length="2093" mass="225154" checksum="C3198EC819967045" modified="2020-08-12" version="1">MSRAVKSVLCQKCDTYTEHDDAKPTSTRSEGEDDGYLVLVRSEAGRLVDDVLEQSVSIVSSSQGPGPMTIDRGLQQPSESEQYAIRSDILSSTDGDLVVKSPTIESMSGKSFDDNMSNPEYDALLLGTAGGQPMSRALGADDTAAEDEGSDAKLTADGADTVATATKDQAAESARRAKKIDRKYARLSADLGPEALLDKNQEYDEAISQIKDEVSMLQSEYSKMSWDESVSMTTGDFGSSTPDNDLQETDLQQQTENNIPQTTTPRLVSQTVEAAAPPASAVSDAFVSGQCVAIAATSTSTVTTTAANLLTDANDVATADQCFTASAAIVERTEQSVLSTTTSTIGSISNAEQSFSIASTEPDRPSESSSSGGGGGGFDGQPVPKPRTSISSRTDTATRSSLLESFEHQDSIEQYPEPAPVQSAEPDLRLRQGSITSDELSNVAQKDSSELVTSEEDISSGAKFFIGECSSDIITRSSTEKRSDFAFDNEGYTFSQESQGTDDQESRRDAMEAYQQQIKLEDGFTLDSLKELDIAGLQSSGKPPVVHSPVTAPATVQPFAETQSVKSEVDVGEATILESTTTEQQEQQQQYAHMTIDELTIAKTLEEVKESLDAVQEELIEAVTDGTPIKQSPSEFEFKILPSTRYVQDPIYETNQEEAVAVSTATAAAITTTTIVSESGLSDSETRSLAAAASAPPIKIDEAPTAAGSSDSSQLEASPRLRHGPAAAKGNRWSATDVDSSGESHYQSFEHTDSSRPLSSDLEQVLPYASSEYETALDHSMVPTSLGTEYHSAVSTLHSYAVSSHDSMKSFDSESSGNLASIESEATETLVPSTMDVDFDSSDAALIHDDSEDDLRDKLLLLDDKEEPSSAASSSVPIAMKRSHEMDFAELKGESVADESLLQFGEQRSHGVESVSSSFQEEKLLSSSIGTARDLVEAMQATSLEDVKTEPADDGKLGTSLEDGSILSISLSSASNLETIMENLSDKTTSAGPVPTHVEIGLDAVTPTTAGTTAGYIGDITLTSTTLKEGDVNFLNTQATTEMIEVSESAELIAGREDSEETVRKRGHKRTESTAIISGKFMAEMGENRDSIESQDESLSQDASKPPTISSERDETREESSDSDYDRYESEYARSFRAPVAQPKKKDKKAGDVFEKDFELDRRNSFSPSQSVIETIVEDVHAEIEQGDEAQQIMVSKSQRLQEYRESSTHNIPDIHVTEDFAEQQQQQQSSDGEDEAKREATVFSETVSVVETVTKTQADATVSSSATTVRKVTTETASTSAGMTMAKQTGIQYAKQEEFQLTEEQYQELIEQKYKAKLADSTTKYAFDADDKDDSAGSDSFEMLEQPDISDEFVIVEEVAREAHEFDAEGKSVAIKPTKIEKKHDEDVEKILVKSAPAHTNAGSLYAANMRDDMMYEFEESPPTGGAEGVDGGAGVTMDLLNNGYPLEESKRWVEMQLAETQNFRYPYEDRLEDIKEEDTDFEVGSSRIGSIKDSFSSTPDYDLLAKRMASREHDDISMSSLQEFENLEHVISLENRKMQQGSQDSLSNGSFTRRFMQRGMHGDDISLSSLKEFEGLENACLEAHLIEIKAKEEAALLLSRSDESNKSNGSNGAKRSPPSNGAAAVGSRQQSTEGAAATKIISSSTSITTTVVGGAVGSSVPSGEMTKVERKITTASGSGREGSSTTTVTTITHVEGQTRQGFDEEDSTSHHILTVSSDSLDGTRVKQPLASGRDAMPSAHSSSDSLDMHSKNNVDPMTSSIDSIEFSKTGIATTRSSQSDSIEQMAQQQQQPNRSDSTDSIEAQQQLQQQQKLQHHQTYHQTTTQQTLSDTKRDSLESLAASSSDNQSGFSSPTKSAHGSRPLSDSGVGASGTTAHHQQQQQQQQFHHQTTTMSTHTVSTGGRTMQKDISADSLTGPDAAFLTSTESLETSSTATNATYQNETDSQMSSSVTSCDSITMVDTVGPSQSLEHWDSSFTAGVHGGGFGASTLMTSSTMSAYSSGVVGSSGGTTIQQQQQQHAFRTSHAEASSSIRMSSSSSSSSTVTMVASSASSTSVRTAQQQLMGEREIAFDDVKETSKERMQSKSTEKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VBK3</accession>
    <name>A0A6E8VBK3_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">NAB co-repressor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NAB family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VBK3"/>
    <dbReference type="EnsemblMetazoa" id="ACON002814-RA">
        <property type="protein sequence ID" value="ACON002814-PA"/>
        <property type="gene ID" value="ACON002814"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002195"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002814"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041985"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.2010:FF:000001">
        <property type="entry name" value="NGFI-A-binding protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.2010">
        <property type="entry name" value="NAB conserved domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006989">
        <property type="entry name" value="NAB_co-repressor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039040">
        <property type="entry name" value="NAB_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006988">
        <property type="entry name" value="Nab_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038398">
        <property type="entry name" value="NCD2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12623">
        <property type="entry name" value="NGFI-A BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12623:SF10">
        <property type="entry name" value="NGFI-A-BINDING PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04905">
        <property type="entry name" value="NCD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04904">
        <property type="entry name" value="SAM_NCD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Nab N-terminal" evidence="8">
        <location>
            <begin position="158"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="NAB co-repressor" evidence="9">
        <location>
            <begin position="332"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="54"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="293"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="487"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="540"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="76"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="303"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="317"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="497"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="543"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="560"/>
            <end position="574"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002814-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="628" mass="66115" checksum="431FCC70DAC6FD97" modified="2020-08-12" version="1">MELKPRSASPTTVPSGVIVQSALAAGSLPEQIGTESAASSSLLAGSSSCTSPLASTVGQVGHLSDRASEPFGSAGGTATTTSTGSSTVAPAKVAPSALSSSCSAPIAVHGKRSPSIATPVKSSNVSGGSSSCIPSPILSPPGKIFGRNNNGIMVVTSTPSNEAELQLYRVLQRASLLNYYDTLLEMGGDDVQQLCDAGEEEFLEIMALVGMASKPLHVRRLQKALHEWMTNPSAFQSPLTNVDIPSRIPYSPEPGSIARSVSTASTGPYGGPSTVAGSFSNLTANAVASTNLPLTPPTPIPSAPASSSNLSSTISNQIGSTTSGSVQLTPSLTEDQVAKIVQAAERLSRTLPRLEPRQQSIKKRSARDLEAVIAMSESDPRRMDEIRKYSAIYGRFDCKRRPEKPLTLHEVCVNEAAAQICKYIPALLTRRDELFPLARQVVRDCGFGHSASIRLTGLSSPLFRSICSQTQPNRGDELDTVQQLKRARLSSDTSSDMGKENSDDNRDISSGSIYQQISDKPFDLTDSSKFSYTRSEFDDTDSRFSFSNSSSSPVQAGNGADRDLSDPDFGELRSHTPRVLDPTGGSLGVQVISSSGGHIIAVANPALALSPALSEAISIKRETISPDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VE05</accession>
    <name>A0A6E8VE05_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VE05"/>
    <dbReference type="EnsemblMetazoa" id="ACON000385-RA">
        <property type="protein sequence ID" value="ACON000385-PA"/>
        <property type="gene ID" value="ACON000385"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005916"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000385"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042948"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032062">
        <property type="entry name" value="DUF4803"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47890">
        <property type="entry name" value="LD24308P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47890:SF1">
        <property type="entry name" value="LD24308P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16061">
        <property type="entry name" value="DUF4803"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026098192" evidence="1">
        <location>
            <begin position="23"/>
            <end position="672"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000385-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="672" mass="75926" checksum="E82E54DA1C1AE037" modified="2020-08-12" version="1">MKMLLPVVTVLLVLVLAPAGHGVAIGPGWLALSETTKVLLRIAVDVAEMQLGDSKPSPINFNQDLRIMHTIQTLSAEMRESQTAMVDILGQRVHDVIESFGHRNRLVERLEKLYMIVRLVERREASMYQLLRTDTPVEQLTLTQFAQATVDVSKSDSVIELLHNLELLVTGSTQVLLPPGRQDIFSQLNETARSVLCSFGLSPQQLIYVLYESIATTELKGYIMMQFSYMLLKTQGQGNFTVESQARRNELQTRLARTQQLVRNVMNQTSGEYWRCDPDRGAHRENVTYVQFTRLVQGYVENEVDMTTDNTCRETCSHYKYGHEQHQCYKELYCSQQPKCAGRIYDCEYIDSDMWICPAAPGGTRRYEYIEYENGEMRGRKQSCPRGTTKVDSWWRWLFWHCSYCFCYCDDAGRHSDRYVSLRESVSDMEQNRVVTGLRFVKRRQMVHLIVQQGLLLPGGEIANSTLEWVVPKPFTHTDKGVRDGHDYHTLSYDRRAIDLDDVHVPPGYVVTGVQFRVLGARLNLLLRMTEMNFTAGKLVGLDASIWSGSLETERTEIPLKYMDVPMLAPAKSVPMSTQNQYLRFGPTGGRADAAQTTIPFLDAQPVSPVRPTPLAGAGLMYKGVPKYGGFVAPKIMTYNFGAHIQDPTDDWFAAELEARSSAAAGGDSDSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VFR3</accession>
    <name>A0A6E8VFR3_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Gustatory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Plays a role in the sugar gustatory response.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the insect chemoreceptor superfamily. Gustatory receptor (GR) family. Gr5a subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VFR3"/>
    <dbReference type="EnsemblMetazoa" id="ACON003259-RB">
        <property type="protein sequence ID" value="ACON003259-PB"/>
        <property type="gene ID" value="ACON003259"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006852"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003259"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033566"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033041">
        <property type="term" value="F:sweet taste receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009318">
        <property type="entry name" value="Gustatory_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21421">
        <property type="entry name" value="GUSTATORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21421:SF34">
        <property type="entry name" value="GUSTATORY RECEPTOR FOR SUGAR TASTE 61A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06151">
        <property type="entry name" value="Trehalose_recp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038981">
        <property type="entry name" value="GRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 8" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="82"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="118"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="165"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="230"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR038981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003259-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="439" mass="50325" checksum="05AACC1C7146D3D4" modified="2020-08-12" version="1">MIEVGFGFRMVPGPVRSSNTTRPTRSRWHWLRKRGNVTFVRPAAEIDDRKERFYLAIASVFPLSNITATDPGAFRFHYFSPYIVLSALSIVGGLFIMAAALVRLNRVGINAMNIAEPIFFGMCTLLQLLFVRLAQAWRGFMVYWAEREEMFFARPYGAINLRRKVIGLAVCILTSALVEHVVYVINQAYNVYQESLTCQYNVTNPLKLYGTLTFGSVYQSVPYHHLLTMYLLYTTISLTFIWTFTDLFIMLVATGIACRFGQLNKRIDSNLQNGSEAFWGEMRTHFVGLIELVERTNRIVGPLLIASCANDMYFLCLQTLNALEDKPYDINDWYFRYSFTFLILRTSVKLWFAADVDENSVRTHKLVQKIRSEHYNDELEILRICSSGGVSISGMGFFTITRRIFLTMAGSILTYELVLMRFHRSSKGVGEDLPCGYID</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VG11</accession>
    <name>A0A6E8VG11_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VG11"/>
    <dbReference type="EnsemblMetazoa" id="ACON003378-RA">
        <property type="protein sequence ID" value="ACON003378-PA"/>
        <property type="gene ID" value="ACON003378"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_008205"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003378"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041650"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042302">
        <property type="term" value="F:structural constituent of cuticle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031311">
        <property type="entry name" value="CHIT_BIND_RR_consensus"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000618">
        <property type="entry name" value="Insect_cuticle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051217">
        <property type="entry name" value="Insect_Cuticle_Struc_Prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12236:SF86">
        <property type="entry name" value="CCP84AC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12236">
        <property type="entry name" value="STRUCTURAL CONTITUENT OF CUTICLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00379">
        <property type="entry name" value="Chitin_bind_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00947">
        <property type="entry name" value="CUTICLE"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00233">
        <property type="entry name" value="CHIT_BIND_RR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51155">
        <property type="entry name" value="CHIT_BIND_RR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0193">Cuticle</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003378-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="113" mass="11724" checksum="03F3E33A94E0B2C8" modified="2020-08-12" version="1">MFGAHGAVVPVAPAVAVSTNYDPLPQYTYAYNVQDALTGDSKSQQETRDGDIVRGSYSLVEPDGTLRTVFYTADPVNGFNAVVQRGPLVPKAVVPVAAGPAILAPAPVARVLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VG17</accession>
    <name>A0A6E8VG17_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">pseudouridine 5'-phosphatase</fullName>
            <ecNumber evidence="7">3.1.3.96</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Pseudouridine-5'-monophosphatase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>psi-UMP + H2O = pseudouridine + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10944"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17802"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58380"/>
            <dbReference type="EC" id="3.1.3.96"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.96" evidence="7"/>
    <dbReference type="RefSeq" id="XP_040225702.1">
        <property type="nucleotide sequence ID" value="XM_040369768.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VG17"/>
    <dbReference type="EnsemblMetazoa" id="ACON003372-RA">
        <property type="protein sequence ID" value="ACON003372-PA"/>
        <property type="gene ID" value="ACON003372"/>
    </dbReference>
    <dbReference type="GeneID" id="120951201"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010493"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003372"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035521"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990738">
        <property type="term" value="F:pseudouridine 5'-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07529">
        <property type="entry name" value="HAD_AtGPP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.240:FF:000001">
        <property type="entry name" value="Haloacid dehalogenase-like hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000055">
        <property type="entry name" value="Haloacid dehalogenase-like hydrolase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="entry name" value="Putative phosphatase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045228">
        <property type="entry name" value="Gpp1/Gpp2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18901">
        <property type="entry name" value="2-DEOXYGLUCOSE-6-PHOSPHATE PHOSPHATASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18901:SF38">
        <property type="entry name" value="PSEUDOURIDINE-5'-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01135">
        <property type="entry name" value="C1.5.6:_HAD__Beta-PGM__Phospha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00052504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00066578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00083904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003372-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="248" mass="27260" checksum="6C7CB204AE0A61FD" modified="2020-08-12" version="1">MSRLFGSSSGSVLGTAGSKMSSFRKVTHCIFDMDGLLLDTENLYTQVTQSIAEPYGKTYTWEIKQTIMGLQRDEAAEAIVAALELPLTPAEYVEISTERINRVMEQCQLMPGAERLVRHLHQHNVPIALATSSGADSVEVKTKNHRELFELFGHKVMGSSDPDVKEGKPAPDIFLVAADRFPDRPAPDQCLVFEDAPNGVTAAIAAGMQAVMVPDPHIEEDQRKHATVVLKSLEDFHPEQFGLPPFTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VG31</accession>
    <name>A0A6E8VG31_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Enolase-phosphatase E1</fullName>
            <ecNumber evidence="8">3.1.3.77</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">2,3-diketo-5-methylthio-1-phosphopentane phosphatase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Bifunctional enzyme that catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>5-methylsulfanyl-2,3-dioxopentyl phosphate + H2O = 1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + phosphate</text>
            <dbReference type="Rhea" id="RHEA:21700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:49252"/>
            <dbReference type="ChEBI" id="CHEBI:58828"/>
            <dbReference type="EC" id="3.1.3.77"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="8">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8">Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 3/6.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8">Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 4/6.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the HAD-like hydrolase superfamily. MasA/MtnC family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.77" evidence="8"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8VG31"/>
    <dbReference type="SMR" id="A0A6E8VG31"/>
    <dbReference type="EnsemblMetazoa" id="ACON003331-RA">
        <property type="protein sequence ID" value="ACON003331-PA"/>
        <property type="gene ID" value="ACON003331"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012534"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003331"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032102"/>
    <dbReference type="UniPathway" id="UPA00904">
        <property type="reaction ID" value="UER00876"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043874">
        <property type="term" value="F:acireductone synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019509">
        <property type="term" value="P:L-methionine salvage from methylthioadenosine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01629">
        <property type="entry name" value="HAD_EP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.720.60:FF:000004">
        <property type="entry name" value="Enolase-phosphatase E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000079">
        <property type="entry name" value="Enolase-phosphatase E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01681">
        <property type="entry name" value="Salvage_MtnC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03117">
        <property type="entry name" value="Salvage_MtnC_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023943">
        <property type="entry name" value="Enolase-ppase_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027511">
        <property type="entry name" value="ENOPH1_eukaryotes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01691">
        <property type="entry name" value="enolase-ppase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01549">
        <property type="entry name" value="HAD-SF-IA-v1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20371">
        <property type="entry name" value="ENOLASE-PHOSPHATASE E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20371:SF1">
        <property type="entry name" value="ENOLASE-PHOSPHATASE E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01133">
        <property type="entry name" value="C1.5.4:_Enolase-phosphatase_Li"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00044">
        <property type="entry name" value="enolase-phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="1 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 8" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="260"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="153"/>
            <end position="154"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003331-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="295" mass="32682" checksum="52DFD750EEC8E315" modified="2020-08-12" version="1">MAAVVALGANVLSAKSIICDIEGTTTSISFVKDTLFPYALKHVEGYLKNNWNEEATKTVVTALREQAEEDKKAEVEGVVPIPTGDSEDIIPEIVKNVEWQMSLDRKTGSLKTLQGLVWAKGYKDGSIKGHVYDDVQKAFEQWTENGRKIYIYSSGSVDAQKLLFEHSEQGDLLKYLSGHYDTKIGAKREKESYTSILKNIESSPEEALFLTDVYAEAKAAKEAGLNVVLLDRPGNSELSEEERKDFPVIATFSDLSFAAETKEENGGATNGKRKIEETNDDVAEEDKAQVYPNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VG33</accession>
    <name>A0A6E8VG33_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Inorganic pyrophosphatase</fullName>
            <ecNumber evidence="4">3.6.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Pyrophosphate phospho-hydrolase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">Ngousso</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.1" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8VG33"/>
    <dbReference type="EnsemblMetazoa" id="ACON003398-RD">
        <property type="protein sequence ID" value="ACON003398-PD"/>
        <property type="gene ID" value="ACON003398"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON003398-RE">
        <property type="protein sequence ID" value="ACON003398-PE"/>
        <property type="gene ID" value="ACON003398"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001536"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003398"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030944"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004427">
        <property type="term" value="F:inorganic diphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006796">
        <property type="term" value="P:phosphate-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00412">
        <property type="entry name" value="pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.80.10:FF:000004">
        <property type="entry name" value="Inorganic pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.80.10">
        <property type="entry name" value="Inorganic pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008162">
        <property type="entry name" value="Pyrophosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036649">
        <property type="entry name" value="Pyrophosphatase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10286">
        <property type="entry name" value="INORGANIC PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00719">
        <property type="entry name" value="Pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50324">
        <property type="entry name" value="Inorganic pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00387">
        <property type="entry name" value="PPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003398-PD"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="282" mass="31291" checksum="620B8367068C4F6B" modified="2020-08-12" version="1">MTASKYQIAERGAPNSTDYRVYFKNENGQSISPLHDIPLYANDERTVYNMVVEVPRWTNAKMEISLGEGLNPIKQDVKKGKLRFVANCFPHHGYIWNYGAFPQTWENPDHLDADTGCKGDNDPIDVLEIGSRIAKRGEVLQVKILGTIALIDEGETDWKIITINVNDPLVDQVNDIGDVETVFPGLLRASVEWFKIYKIPDGKPENQFAFNGDAKDAAFATKTVAETHRFWQQLVNKEVDNNGISCLNTTVDGSPYLVANDAADEVFAKSATGGNPEPVSEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VHT6</accession>
    <name>A0A6E8VHT6_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="7">MBD domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_040218845.1">
        <property type="nucleotide sequence ID" value="XM_040362911.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VHT6"/>
    <dbReference type="EnsemblMetazoa" id="ACON003982-RA">
        <property type="protein sequence ID" value="ACON003982-PA"/>
        <property type="gene ID" value="ACON003982"/>
    </dbReference>
    <dbReference type="GeneID" id="120947528"/>
    <dbReference type="CTD" id="41151"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005682"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003982"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040842"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008327">
        <property type="term" value="F:methyl-CpG binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006346">
        <property type="term" value="P:DNA methylation-dependent constitutive heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.890.10">
        <property type="entry name" value="Methyl-cpg-binding Protein 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032343">
        <property type="entry name" value="MBD2/MBD3_p55-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025884">
        <property type="entry name" value="MeCpG-bd_2/3_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001739">
        <property type="entry name" value="Methyl_CpG_DNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12396:SF0">
        <property type="entry name" value="METHYL-CPG BINDING DOMAIN PROTEIN-LIKE, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12396">
        <property type="entry name" value="METHYL-CPG BINDING PROTEIN, MBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14048">
        <property type="entry name" value="MBD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16564">
        <property type="entry name" value="MBDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00391">
        <property type="entry name" value="MBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50982">
        <property type="entry name" value="MBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MBD" evidence="6">
        <location>
            <begin position="5"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003982-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="203" mass="22903" checksum="D4A750905D459405" modified="2020-08-12" version="1">MNVSIERKRTDCAALPKGWQREEVLRKTGLSAGKVDVYYYSRAMRSDTSLIPPIRQTASIFKQPVTVVRSQEPDNAKAKRELQHGSQVKPKQLFWEKRLENLGASNTHNEEIGSIELPKRLRPIGPGIRETTVLQSLATALHHNTLPVTGQTASKTSLNTNAGVFTNPHQPLMTNVTITEEDVKRQEERVQYARLRLQEALRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VHT7</accession>
    <name>A0A6E8VHT7_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Annexin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="domain">
        <text evidence="6">A pair of annexin repeats may form one binding site for calcium and phospholipid.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the annexin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_049463110.1">
        <property type="nucleotide sequence ID" value="XM_049607153.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VHT7"/>
    <dbReference type="EnsemblMetazoa" id="ACON003790-RB">
        <property type="protein sequence ID" value="ACON003790-PB"/>
        <property type="gene ID" value="ACON003790"/>
    </dbReference>
    <dbReference type="GeneID" id="120947902"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004607"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003790"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_029479"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012506">
        <property type="term" value="C:vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001786">
        <property type="term" value="F:phosphatidylserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032509">
        <property type="term" value="P:endosome transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000001">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000002">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000004">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000010">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.10">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001464">
        <property type="entry name" value="Annexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018502">
        <property type="entry name" value="Annexin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018252">
        <property type="entry name" value="Annexin_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037104">
        <property type="entry name" value="Annexin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10502">
        <property type="entry name" value="ANNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10502:SF233">
        <property type="entry name" value="ANNEXIN B9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00191">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00196">
        <property type="entry name" value="ANNEXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00335">
        <property type="entry name" value="ANX"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47874">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00223">
        <property type="entry name" value="ANNEXIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51897">
        <property type="entry name" value="ANNEXIN_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0041">Annexin</keyword>
    <keyword evidence="3 6" id="KW-0106">Calcium</keyword>
    <keyword evidence="5 6" id="KW-0111">Calcium/phospholipid-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003790-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="324" mass="35861" checksum="9F06BDCC4E858650" modified="2020-08-12" version="1">MSSSQYYPFQCTPTVYPADPFDANEDAATLRTAMKGFGTDEKAIIEVLARRGIVQRLEIAQAFKTSFGKDLISDLKSELGGKFEDVILALMTPLPQFYAKELHDAISGIGTDEEAIIEILCTLSNYGIRTIAEFYEQMYNVSLESDLKGDTSGAFKRLCVSLVQGNRDENNGVDEGAAAADAQALFEAGEGQWGTDESVFNQILVTRSYQQLRAVFDIYESLAGHSIEDAIKREFSGAIEEGFKAIVRCVRSKVQYFAKRLHNSMAGLGTNDKTLIRIIVSRSEIDLGDIKEAFQEMYGKSLESWIKEDLGGDNANLLYHLAAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VHV2</accession>
    <name>A0A6E8VHV2_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8VHV2"/>
    <dbReference type="EnsemblMetazoa" id="ACON003997-RC">
        <property type="protein sequence ID" value="ACON003997-PC"/>
        <property type="gene ID" value="ACON003997"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007284"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003997"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030304"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032880">
        <property type="term" value="P:regulation of protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14126">
        <property type="entry name" value="STKc_CK1_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000703">
        <property type="entry name" value="Casein kinase I gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022247">
        <property type="entry name" value="Casein_kinase-1_gamma_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12605">
        <property type="entry name" value="CK1gamma_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 13 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="97"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="406"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="10"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="15">
        <location>
            <begin position="56"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="430"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003997-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="465" mass="51729" checksum="3D743F1FBCE58E70" modified="2023-06-28" version="2">MQRRGEGKDQQQQQQQPSQPARAAGEAGVAGGGGRDRDVARQTSGVPGAGASNTAAGGGGGGGGGGGGGGARGGSSMYSARHSVSSSSGVLMVGPNFRVGKKIGCGNFGELRLGKNLYNNEHVAIKMESMKSKAPQLHLEYRFYKLLGAHAEGIPEVYYFGPCGKYNALVMELLGPSLEDLFDLCGRRFSLKTVLMIAIQLLHRIEYVHSRHLIYRDVKPENFLIGRNSNKRDKIIHIIDFGLAKEYIDLDTNKHIPYREHKSLTGTARYMSINTHMGKEQSRRDDLEALGHMFMYFLRGSLPWQGLKADTLKERYQKIGDTKRATPIDVLCDGHPEEFAKYLRYVRRLDFFETPDYDHLRRLFQDLFYFKGYVDDGEFDWTGKTMSTPVGSLQTGHEVVVSPNRERHTPANKVVSSTNGELNADDPTAGHSNTPITQQPEVELVDETKCCCFFKRKKKKSSRQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VHV3</accession>
    <name>A0A6E8VHV3_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8VHV3"/>
    <dbReference type="EnsemblMetazoa" id="ACON003997-RB">
        <property type="protein sequence ID" value="ACON003997-PB"/>
        <property type="gene ID" value="ACON003997"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007284"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003997"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030304"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032880">
        <property type="term" value="P:regulation of protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14126">
        <property type="entry name" value="STKc_CK1_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000703">
        <property type="entry name" value="Casein kinase I gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022247">
        <property type="entry name" value="Casein_kinase-1_gamma_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12605">
        <property type="entry name" value="CK1gamma_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 13 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="59"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="17"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="375"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="15">
        <location>
            <begin position="18"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="432"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003997-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="467" mass="51931" checksum="B4416D35F0468A4F" modified="2023-06-28" version="2">MRLYICGVPGAGASNTAAGGGGGGGGGGGGGGARGGSSMYSARHSVSSSSGVLMVGPNFRVGKKIGCGNFGELRLGKNLYNNEHVAIKMESMKSKAPQLHLEYRFYKLLGAHDTQSPPEGIPRVYHLGTCGGRYNAMVLELLGPSLEDLFTLCGRKFSLKTVLMIAKQLLHRIEYVHSRHLIYRDVKPENFLIGRNSNKRDKIIHIIDFGLAKEYIDLDTNKHIPYREHKSLTGTARYMSINTHMGKEQSRRDDLEALGHMFMYFLRGSLPWQGLKADTLKERYQKIGDTKRATPIDVLCDGHPEEFAKYLRYVRRLDFFETPDYDHLRRLFQDLFYFKGYVDDGEFDWTGKTMSTPVGSLQTGHEVVVSPNRERHTPANKQTNSKGGVAAWPDVPKQTGTLGNLTPADRHGSVQVVSSTNGELNADDPTAGHSNTPITQQPEVELVDETKCCCFFKRKKKKSSRQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VI53</accession>
    <name>A0A6E8VI53_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VI53"/>
    <dbReference type="EnsemblMetazoa" id="ACON004107-RB">
        <property type="protein sequence ID" value="ACON004107-PB"/>
        <property type="gene ID" value="ACON004107"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON004107-RC">
        <property type="protein sequence ID" value="ACON004107-PC"/>
        <property type="gene ID" value="ACON004107"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009202"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004107"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033639"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24064">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 22 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="27"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="108"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="166"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="193"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="227"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="260"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="302"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="312"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004107-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="348" mass="38759" checksum="DD5A3B706FB8C6AC" modified="2020-08-12" version="1">MDTPDDSYDEIMAQAGNDGKFQRRYNLLFNFGAICFASMSYMSIILALNKPPHNCFVPGMEQYNITDANLWKNLTLPRETDNRGQLGYSKCQMYNITEQHLQRHYSEWSFASSDIITVCSVTYFTLVLLSSRMDGNPFLNFMYQGLIEVPAAFAGQRAADRFGRRATNAMALCCATAVCLVLVLIVRDPANEMAATVLTTFVKFCISITFFGVNLQSIEIYPTCLRQTGMAVGTIAATIFGIVGPYVVHLGTEYDVRYPFLVMGLSSAVGILAALFLPETLHQSLPNTIEEAQKFGKNQRFWSLPKAPQVTREADSNTDKRHDGSDAKPEESIRLTVPVEAEEAVGRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VI54</accession>
    <name>A0A6E8VI54_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="9">C-terminal binding protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VI54"/>
    <dbReference type="EnsemblMetazoa" id="ACON003893-RD">
        <property type="protein sequence ID" value="ACON003893-PD"/>
        <property type="gene ID" value="ACON003893"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035290"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140297">
        <property type="term" value="F:DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001221">
        <property type="term" value="F:transcription coregulator binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05299">
        <property type="entry name" value="CtBP_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000012">
        <property type="entry name" value="C-terminal-binding protein 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043322">
        <property type="entry name" value="CtBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051638">
        <property type="entry name" value="CTBP_dehydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029753">
        <property type="entry name" value="D-isomer_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46029">
        <property type="entry name" value="C-TERMINAL-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46029:SF7">
        <property type="entry name" value="C-TERMINAL-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00671">
        <property type="entry name" value="D_2_HYDROXYACID_DH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase catalytic" evidence="7">
        <location>
            <begin position="34"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding" evidence="8">
        <location>
            <begin position="130"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="359"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="377"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="389"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003893-PD"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="414" mass="44403" checksum="14B398A949EEC2C8" modified="2023-06-28" version="1">MMPKRSRLGDVRGPISNGPMQSRPLVALLDGRDCSIEMPILKDVATVAFCDAQSTSEIHEKVLNEAVGALMWHTIILTKEDLEKFKALRIIVRIGSGVDNIDVKAAGELGIAVCNVPGYGVEEVADTTMCLILNLYRRTYWLANMVREGKKFTGPEQVRDAAQGCARIRGDTLGLVGLGRIGSAVALRAKVFGFNVSFYDPYLPDGIEKSLGLNRVYTLQELLFHSDCVSLHCTLNEHNHHLINEFTIKQMRPGAFLVNTARGGLVDDEALAVALKQGRIRAAALDVHENEPYNGALKDAPNLLCTPHAAFYSEAATTELREMAASEIRRAIIGNIPECLRNCVNKEYFLRSSTGGGAGGYSEGGLQQAHSTTPLEAPHSAGSHAPPSGGGPPPPPVAVIPPVSAVTAPPPQAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VJX0</accession>
    <name>A0A6E8VJX0_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Sodium channel protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 18">Cell membrane</location>
            <topology evidence="1 18">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the sodium channel (TC 1.A.1.10) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON004707-RE">
        <property type="protein sequence ID" value="ACON004707-PE"/>
        <property type="gene ID" value="ACON004707"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002271"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004707"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035906"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001518">
        <property type="term" value="C:voltage-gated sodium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005248">
        <property type="term" value="F:voltage-gated sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0086010">
        <property type="term" value="P:membrane depolarization during action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019228">
        <property type="term" value="P:neuronal action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13433">
        <property type="entry name" value="Na_channel_gate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000061">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000046">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000047">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000019">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000022">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000023">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000026">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058542">
        <property type="entry name" value="IQ_SCN5A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001696">
        <property type="entry name" value="Na_channel_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044564">
        <property type="entry name" value="Na_chnl_inactivation_gate"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010526">
        <property type="entry name" value="Na_trans_assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024583">
        <property type="entry name" value="Na_trans_cytopl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043203">
        <property type="entry name" value="VGCC_Ca_Na"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10037:SF288">
        <property type="entry name" value="SODIUM CHANNEL PROTEIN PARA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10037">
        <property type="entry name" value="VOLTAGE-GATED CATION CHANNEL CALCIUM AND SODIUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24609">
        <property type="entry name" value="IQ_SCN5A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06512">
        <property type="entry name" value="Na_trans_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11933">
        <property type="entry name" value="Na_trans_cytopl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00170">
        <property type="entry name" value="NACHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="17 18" id="KW-0407">Ion channel</keyword>
    <keyword evidence="12 18" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0691">RNA editing</keyword>
    <keyword evidence="11 18" id="KW-0915">Sodium</keyword>
    <keyword evidence="3 18" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="16 18" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="7 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 18" id="KW-0813">Transport</keyword>
    <keyword evidence="9 18" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="133"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="204"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="262"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="707"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="751"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="780"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="835"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="915"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1215"/>
            <end position="1237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1243"/>
            <end position="1269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1290"/>
            <end position="1321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1408"/>
            <end position="1434"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1497"/>
            <end position="1515"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1527"/>
            <end position="1546"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1558"/>
            <end position="1577"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1621"/>
            <end position="1644"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1714"/>
            <end position="1734"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="20">
        <location>
            <begin position="1751"/>
            <end position="1786"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="34"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1046"/>
            <end position="1116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1901"/>
            <end position="1976"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="34"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="52"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="1073"/>
            <end position="1099"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="1100"/>
            <end position="1116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="19">
        <location>
            <begin position="1913"/>
            <end position="1941"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU361132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004707-PE"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="2009" mass="225712" checksum="5D8A366B24E48706" modified="2020-08-12" version="1">MTEDSDSISEEERSLFRPFTRESLQAIEARIADEEAKQRELERKRAEGEIRYDDEDEDEGPQPDPTLEQGVPVPVRMQGSFPPELASTPLEDIDSFYSNQRTFVVISKGKDIFRFSATNALYVLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILQPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKEFPLDGSWGNLTDESWELFNSNDTNWFYSESGDIPLCGNSSGAGQCDEGYICLQGYGKNPNYGYTSFDTFGWAFLSAFRLMTQDYWENLYQLEAEEAAAAKAAKLEAQQAAAAAAANPEIAKSPSDFSCHSYELFVGQEKGNDDNNKEKMSIRSEGLESVSEITRTTAPTATAAGTAKARKVSAFTIRNGRGRFVGVPGSDRKPLVLSTYLDAQEHLPYADDSNAVTPMSEENGAIIVPVYYANLGSRHSSYTSHQSRISYTSHGDLLGGMTKESRLRNRSARNTNHSIVPPPNANNLSYAETNHKGQRDFDLTQDCTDDAGKIKHNDNPFIEPAQTQTVVDMKDVMVLNDIIEQAAGRHSRASDHGVSVYYFPTEDDDEDGPTFKDKALEFLMKMIDIFCVWDCCWVWLKFQEWVAFIVFDPFVELFITLCIVVNTLFMALDHHDMDPDMEKALKSGNYFFTATFAIEATMKLIAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYVDNVDRFPDHDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMLVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNETNKIAEAFNRISRFSNWIKMNLANALKFVKNKLTSQIASVQPTEHGENELELTPDDILADGLLKKGIKEHNQLEVAIGDGMEFTIHGDLKNKAKKNKQIMNNSKDDDTASIKSYGSHKNRPFKDESHKGSAETMEGEEKRDASKEDLGIDEELDDEGEGDEGPLDGELIIHAEEDEVIEDSPADCCPDNCYKKFPVLAGDDDAPFWQGWGNLRLKTFQLIENKYFETAVITMILLSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFIIVMVSLINFVASLCGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCVDKNKTTLPHEIIPDVNACKAENYSWENSPMNFDHVGKAYLCLFQVATFKGWIQIMNDAIDSRDVGKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTNKKFDMIIMLFIGFNMLTMTLDHYKQSETFSAVLDYLNMIFICIFSSECLMKIFALRYHYFIEPWNLFDFVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKDKSGLDDVYNFKTFGQSMILLFQMSTSAGWDGVLDGIINEEDCLPPDNDKGYPGNCGSSTIGITYLLAYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPDGTQYVRYDQLSDFLDVLEPPLQIHKPNRYKIISMDIPICRGDMMFCVDILDALTKDFFARKGNPIEETAELGEVQQRPDEVGYEPVSSTLWRQREEYCARLIQHAWKRYKQRHGGGTDASGDDLEIDACDNGCGGGNGNENDDSGDGATGSGDNGSQHGGGSISGGGGTPGGGKSKGIIGSTQANIGIVDSNISPKESPDSIGDPQGRQTAVLVESDGFVTKNGHRVVIHSRSPSITSRTADV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VJX3</accession>
    <name>A0A6E8VJX3_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Sodium channel protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="22">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 18">Cell membrane</location>
            <topology evidence="1 18">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the sodium channel (TC 1.A.1.10) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON004707-RJ">
        <property type="protein sequence ID" value="ACON004707-PJ"/>
        <property type="gene ID" value="ACON004707"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010356"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004707"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034190"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001518">
        <property type="term" value="C:voltage-gated sodium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005248">
        <property type="term" value="F:voltage-gated sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0086010">
        <property type="term" value="P:membrane depolarization during action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:neuronal action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Na_channel_gate"/>
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    </dbReference>
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        <property type="entry name" value="SODIUM CHANNEL PROTEIN PARA"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="NACHANNEL"/>
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        <property type="match status" value="4"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="17 18" id="KW-0407">Ion channel</keyword>
    <keyword evidence="12 18" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0691">RNA editing</keyword>
    <keyword evidence="11 18" id="KW-0915">Sodium</keyword>
    <keyword evidence="3 18" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="16 18" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="7 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="144"/>
            <end position="171"/>
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            <begin position="215"/>
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    <feature type="transmembrane region" description="Helical" evidence="18">
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    <feature type="transmembrane region" description="Helical" evidence="18">
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    <feature type="transmembrane region" description="Helical" evidence="18">
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            <begin position="404"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
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    <feature type="transmembrane region" description="Helical" evidence="18">
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    <feature type="transmembrane region" description="Helical" evidence="18">
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    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="981"/>
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            <begin position="1291"/>
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        </location>
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    </feature>
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        <location>
            <begin position="1989"/>
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        </location>
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        <source>
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        </source>
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        </source>
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        </source>
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            <dbReference type="RuleBase" id="RU361132"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
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            <dbReference type="EnsemblMetazoa" id="ACON004707-PJ"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="2085" mass="234274" checksum="C3DBD04F39B563F4" modified="2020-08-12" version="1">MTEDSDSISEEERSLFRPFTRESLQAIEARIADEEAKQRELERKRAEGESDFGRKKKKKEIRYDDEDEDEGPQPDPTLEQGVPVPVRMQGSFPPELASTPLEDIDSFYSNQRTFVVISKGKDIFRFSATNALYVLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILQPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKEFPLDGSWGNLTDESWELFNSNDTNWFYSESGDIPLCGNSSGAGQCDEGYICLQGYGKNPNYGYTSFDTFGWAFLSAFRLMTQDYWENLYQLVLRSAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEALREAEEAAAAKAAKLEAQQAAAAAAANPEIAKSPSDFSCHSYELFVGQEKGNDDNNKEKMSIRSEGLESVSEITRTTAPTATAAGTAKARKVSAFTIRNGRGRFVGVPGSDRKPLVLSTYLDAQEHLPYADDSNAVTPMSEENGAIIVPVYYANLGSRHSSYTSHQSRISYTSHGDLLGGMTKESRLRNRSARNTNHSIVPPPNANNLSYAETNHKGQRDFDLTQDCTDDAGKIKHNDNPFIEPAQTQTVVDMKDVMVLNDIIEQAAGRHSRASDHGVSVYYFPTEDDDEDGPTFKDKALEFLMKMIDIFCVWDCCWVWLKFQEWVAFIVFDPFVELFITLCIVVNTLFMALDHHDMDPDMEKALKSGNYFFTATFAIEATMKLIAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYVDNVDRFPDHDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMLVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNETNKIAEAFNRISRFSNWIKMNLANALKFVKNKLTSQIASVQPTGKGVCPCISSEHGENELELTPDDILADGLLKKGIKEHNQLEVAIGDGMEFTIHGDLKNKAKKNKQIMNNSKDDDTASIKSYGSHKNRPFKDESHKGSAETMEGEEKRDASKEDLGIDEELDDEGEGDEGPLDGELIIHAEEDEVIEDSPADCCPDNCYKKFPVLAGDDDAPFWQGWGNLRLKTFQLIENKYFETAVITMILLSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFIIVMVSLINFVASLCGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCVDKNKTTLPHEIIPDVNACKAENYSWENSPMNFDHVGKAYLCLFQVATFKGWIQIMNDAIDSRDVGKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTNKKFDMIIMLFIGFNMLTMTLDHYKQSETFSAVLDYLNMIFICIFSSECLMKIFALRYHYFIEPWNLFDFVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKDKSGLDDVYNFKTFGQSMILLFQMSTSAGWDGVLDGIINEEDCLPPDNDKGYPGNCGSSTIGITYLLAYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPDGTQYVRYDQLSDFLDVLEPPLQIHKPNRYKIISMDIPICRGDMMFCVDILDALTKDFFARKGNPIEETAELGEVQQRPDEVGYEPVSSTLWRQREEYCARLIQHAWKRYKQRHGGGTDASGDDLEIDACDNGCGGGNGNENDDSGDGATGSGDNGSQHGGGSISGGGGTPGGGKSKGIIGSTQANIGIVDSNISPKESPDSIGDPQGRQTAVLVESDGFVTKNGHRVVIHSRSPSITSRTADV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VJY7</accession>
    <name>A0A6E8VJY7_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Proteasomal ubiquitin receptor ADRM1 homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">May function as a proteasomal ubiquitin receptor. May promote the deubiquitinating activity associated with the 26S proteasome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ADRM1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VJY7"/>
    <dbReference type="EnsemblMetazoa" id="ACON004758-RB">
        <property type="protein sequence ID" value="ACON004758-PB"/>
        <property type="gene ID" value="ACON004758"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012481"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004758"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041481"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061133">
        <property type="term" value="F:endopeptidase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070628">
        <property type="term" value="F:proteasome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13314">
        <property type="entry name" value="PH_Rpn13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.70:FF:000001">
        <property type="entry name" value="Proteasomal ubiquitin receptor ADRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.2020.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.70">
        <property type="entry name" value="Proteasomal ubiquitin receptor Rpn13/ADRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Rpn13/ADRM1"/>
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        <property type="entry name" value="RPN13_DEUBAD"/>
    </dbReference>
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        <property type="entry name" value="RPN13_DEUBAD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12225">
        <property type="entry name" value="ADHESION REGULATING MOLECULE 1 110 KDA CELL MEMBRANE GLYCOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12225:SF0">
        <property type="entry name" value="PROTEASOMAL UBIQUITIN RECEPTOR ADRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04683">
        <property type="entry name" value="Rpn13_ADRM1_Pru"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RPN13_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51916">
        <property type="entry name" value="DEUBAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51917">
        <property type="entry name" value="PRU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0647">Proteasome</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pru" evidence="11">
        <location>
            <begin position="17"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="DEUBAD" evidence="10">
        <location>
            <begin position="286"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
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            <begin position="130"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="185"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="389"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="136"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="192"/>
            <end position="216"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="228"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="400"/>
            <end position="417"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="421"/>
            <end position="436"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009216"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00054744"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00070663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51916"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51917"/>
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    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004758-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="443" mass="46673" checksum="E87641939B469754" modified="2020-08-12" version="1">MSGPIFGSSSALGGSSGGNRHLVEFRAGRMNMVNKMVHPDNRKGLVYVYQAEDGLIHFCWKDRTSGTVEDDLILFPDDCEFKKIDYVKNGRVYVLKFKSSSRRLFFWMQEPKTDRDDEWCRRINEVINNPPSGNSIGSGGRNGGNDNGDLQYMLNNMSQQQIMQLFGGVGQMGGLSSLLGSMTSRTSAATPANRSSSSLATSNNGNGGSTTTPSAAVNAPSTPRAPKKTSGTTKTNSASGASAGSPISTPVGRNAGSAGTADGGTRILLSDLQNYLSGISATGVDGSGQRRNIDLASAVNSQTLASIISDQEKVDALVAHLPQLEGDENKKEQLKETISSPQFQQALSMFSNALQSGQLGPVVSQFQLNADAVAAANAGDLEQFVKALENSEKSPASEASGATNKQSAGSSTSTDQPKPSEVAKEENGHGKTQNKDQDDEMAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VJY8</accession>
    <name>A0A6E8VJY8_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Sodium channel protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="22">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 18">Cell membrane</location>
            <topology evidence="1 18">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the sodium channel (TC 1.A.1.10) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="SMR" id="A0A6E8VJY8"/>
    <dbReference type="EnsemblMetazoa" id="ACON004707-RD">
        <property type="protein sequence ID" value="ACON004707-PD"/>
        <property type="gene ID" value="ACON004707"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010356"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004707"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034190"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001518">
        <property type="term" value="C:voltage-gated sodium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005248">
        <property type="term" value="F:voltage-gated sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0086010">
        <property type="term" value="P:membrane depolarization during action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019228">
        <property type="term" value="P:neuronal action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13433">
        <property type="entry name" value="Na_channel_gate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000061">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Sodium channel protein"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
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        <property type="entry name" value="EF_hand_dom"/>
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        <property type="entry name" value="Ion_trans_dom"/>
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        <property type="entry name" value="Na_trans_assoc_dom"/>
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        <property type="entry name" value="VGCC_Ca_Na"/>
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        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="SODIUM CHANNEL PROTEIN PARA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10037">
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Na_trans_assoc"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF11933">
        <property type="entry name" value="Na_trans_cytopl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00170">
        <property type="entry name" value="NACHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="17 18" id="KW-0407">Ion channel</keyword>
    <keyword evidence="12 18" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0691">RNA editing</keyword>
    <keyword evidence="11 18" id="KW-0915">Sodium</keyword>
    <keyword evidence="3 18" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="16 18" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="7 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 18" id="KW-0813">Transport</keyword>
    <keyword evidence="9 18" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="127"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="198"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="232"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="256"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="387"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="777"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="821"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="850"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="905"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="985"/>
            <end position="1006"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1321"/>
            <end position="1343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1349"/>
            <end position="1375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1396"/>
            <end position="1427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1517"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1606"/>
            <end position="1624"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1636"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1667"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1730"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1823"/>
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        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="21">
        <location>
            <begin position="1860"/>
            <end position="1895"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
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        <location>
            <begin position="27"/>
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    </feature>
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        <location>
            <begin position="1160"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="2010"/>
            <end position="2054"/>
        </location>
    </feature>
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        <location>
            <begin position="413"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="30"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="1179"/>
            <end position="1205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="20">
        <location>
            <begin position="1206"/>
            <end position="1222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="20">
        <location>
            <begin position="2022"/>
            <end position="2050"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU361132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004707-PD"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="2118" mass="237566" checksum="9843358DB6CF4354" modified="2020-08-12" version="1">MTEDSDSISEEERSLFRPFTRESLQAIEARIADEEAKQRELERKRAEGEDEDEGPQPDPTLEQGVPVPVRMQGSFPPELASTPLEDIDSFYSNQRTFVVISKGKDIFRFSATNALYVLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILQPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKEFPLDGSWGNLTDESWELFNSNDTNWFYSESGDIPLCGNSSGAGQCDEGYICLQGYGKNPNYGYTSFDTFGWAFLSAFRLMTQDYWENLYQLVLRSAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEALREAEEAAAAKAAKLEAQQAAAAAAANPEIAKSPSDFSCHSYELFVGQEKGNDDNNKEKMSIRSEGLESVSEITRTTAPTATAAGTAKARKVSAASLSLPGSPFNLRRGSRGSHQFTIRNGRGRFVGVPGSDRKPLVLSTYLDAQEHLPYADDSNAVTPMSEENGAIIVPVYYANLGSRHSSYTSHQSRISYTSHGDLLGGMTKESRLRNRSARNTNHSIVPPPNANNLSYAETNHKGQRDFDLTQDCTDDAGKIKHNDNPFIEPAQTQTVVDMKDVMVLNDIIEQAAGRHSRASDHGVSVYYFPTEDDDEDGPTFKDKALEFLMKMIDIFCVWDCCWVWLKFQEWVAFIVFDPFVELFITLCIVVNTLFMALDHHDMDPDMEKALKSGNYFFTATFAIEATMKLIAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTVGALGNLTFVLCIIIFIFAVMGMQLFGKNYTDNVDRFPDHDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMLVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNETNKIAEAFNRISRFSNWIKMNLANALKFVKNKLTSQIASVQPTGKGVCPCISSEHGENELELTPDDILADGLLKKGIKEHNQLEVAIGDGMEFTIHGDLKNKAKKNKQIMNNSKVIGNSISNHQDNKLDHELNHRGVSLQDDDTASIKSYGSHKNRPFKDESHKGSAETMEGEEKRDASKEDLGIDEELDDEGEGDEGPLDGELIIHAEEDEVIEDSPADCCPDNCYKKFPVLAGDDDAPFWQGWGNLRLKTFQLIENKYFETAVITMILLSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFIIVMVSLINFVASLCGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCVDKNKTTLPHEIIPDVNACKAENYSWENSPMNFDHVGKAYLCLFQVATFKGWIQIMNDAIDSRDVSFVGKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTNKKFDMIIMLFIGFNMLTMTLDHYKQSETFSAVLDYLNMIFICIFSSECLMKIFALRYHYFIEPWNLFDFVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKDKSGLDDVYNFKTFGQSMILLFQMSTSAGWDGVLDGIINEEDCLPPDNDKGYPGNCGSSTIGITYLLAYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPDGTQYVRYDQLSDFLDVLEPPLQIHKPNRYKIISMDIPICRGDMMFCVDILDALTKDFFARKGNPIEETAELGEVQQRPDEVGYEPVSSTLWRQREEYCARLIQHAWKRYKQRHGGGTDASGDDLEIDACDNGCGGGNGNENDDSGDGATGSGDNGSQHGGGSISGGGGTPGGGKSKGIIGSTQANIGIVDSNISPKESPDSIGDPQGRQTAVLVESDGFVTKNGHRVVIHSRSPSITSRTADV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VJZ3</accession>
    <name>A0A6E8VJZ3_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Sodium channel protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="22">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 18">Cell membrane</location>
            <topology evidence="1 18">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the sodium channel (TC 1.A.1.10) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON004707-RI">
        <property type="protein sequence ID" value="ACON004707-PI"/>
        <property type="gene ID" value="ACON004707"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010356"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004707"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034190"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001518">
        <property type="term" value="C:voltage-gated sodium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005248">
        <property type="term" value="F:voltage-gated sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0086010">
        <property type="term" value="P:membrane depolarization during action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019228">
        <property type="term" value="P:neuronal action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13433">
        <property type="entry name" value="Na_channel_gate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000061">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000046">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000047">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000019">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000022">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000023">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000026">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058542">
        <property type="entry name" value="IQ_SCN5A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001696">
        <property type="entry name" value="Na_channel_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044564">
        <property type="entry name" value="Na_chnl_inactivation_gate"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010526">
        <property type="entry name" value="Na_trans_assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024583">
        <property type="entry name" value="Na_trans_cytopl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043203">
        <property type="entry name" value="VGCC_Ca_Na"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10037:SF288">
        <property type="entry name" value="SODIUM CHANNEL PROTEIN PARA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10037">
        <property type="entry name" value="VOLTAGE-GATED CATION CHANNEL CALCIUM AND SODIUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24609">
        <property type="entry name" value="IQ_SCN5A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06512">
        <property type="entry name" value="Na_trans_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11933">
        <property type="entry name" value="Na_trans_cytopl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00170">
        <property type="entry name" value="NACHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="17 18" id="KW-0407">Ion channel</keyword>
    <keyword evidence="12 18" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0691">RNA editing</keyword>
    <keyword evidence="11 18" id="KW-0915">Sodium</keyword>
    <keyword evidence="3 18" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="16 18" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="7 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 18" id="KW-0813">Transport</keyword>
    <keyword evidence="9 18" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="133"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="204"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="238"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="262"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="393"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="790"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="834"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="863"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="918"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="960"/>
            <end position="977"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="998"/>
            <end position="1019"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1295"/>
            <end position="1313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1334"/>
            <end position="1356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1362"/>
            <end position="1388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1409"/>
            <end position="1440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1527"/>
            <end position="1553"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1616"/>
            <end position="1634"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1646"/>
            <end position="1665"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1677"/>
            <end position="1696"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1740"/>
            <end position="1763"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1833"/>
            <end position="1853"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="21">
        <location>
            <begin position="1870"/>
            <end position="1905"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="34"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1173"/>
            <end position="1235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="2020"/>
            <end position="2064"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="19">
        <location>
            <begin position="419"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="34"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="20">
        <location>
            <begin position="52"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="1192"/>
            <end position="1218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="20">
        <location>
            <begin position="1219"/>
            <end position="1235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="20">
        <location>
            <begin position="2032"/>
            <end position="2060"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU361132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004707-PI"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="2128" mass="238753" checksum="89CEB40D1C87A00B" modified="2020-08-12" version="1">MTEDSDSISEEERSLFRPFTRESLQAIEARIADEEAKQRELERKRAEGEIRYDDEDEDEGPQPDPTLEQGVPVPVRMQGSFPPELASTPLEDIDSFYSNQRTFVVISKGKDIFRFSATNALYVLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILQPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKEFPLDGSWGNLTDESWELFNSNDTNWFYSESGDIPLCGNSSGAGQCDEGYICLQGYGKNPNYGYTSFDTFGWAFLSAFRLMTQDYWENLYQLVLRSAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEALREAEEAAAAKAAKLEAQQAAAAAAANPEIAKSPSDFSCHSYELFVGQEKGNDDNNKEKMSIRSEGLESVSEITRTTAPTATAAGTAKARKVSAGVATIQKASLSLPGSPFNLRRGSRGSHQFTIRNGRGRFVGVPGSDRKPLVLSTYLDAQEHLPYADDSNAVTPMSEENGAIIVPVYYANLGSRHSSYTSHQSRISYTSHGDLLGGMTKESRLRNRSARNTNHSIVPPPNANNLSYAETNHKGQRDFDLTQDCTDDAGKIKHNDNPFIEPAQTQTVVDMKDVMVLNDIIEQAAGRHSRASDHGVSVYYFPTEDDDEDGPTFKDKALEFLMKMIDIFCVWDCCWVWLKFQEWVAFIVFDPFVELFITLCIVVNTLFMALDHHDMDPDMEKALKSGNYFFTATFAIEATMKLIAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYVDNVDRFPDHDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMLVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNETNKIAEAFNRISRFSNWIKMNLANALKFVKNKLTSQIASVQPTGKGVCPCISSEHGENELELTPDDILADGLLKKGIKEHNQLEVAIGDGMEFTIHGDLKNKAKKNKQIMNNSKVIGNSISNHQDNKLDHELNHRGVSLQDDDTASIKSYGSHKNRPFKDESHKGSAETMEGEEKRDASKEDLGIDEELDDEGEGDEGPLDGELIIHAEEDEVIEDSPADCCPDNCYKKFPVLAGDDDAPFWQGWGNLRLKTFQLIENKYFETAVITMILLSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFIIVMVSLINFVASLCGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCVDKNKTTLPHEIIPDVNACKAENYSWENSPMNFDHVGKAYLCLFQVATFKGWIQIMNDAIDSRDVGKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTNKKFDMIIMLFIGFNMLTMTLDHYKQSETFSAVLDYLNMIFICIFSSECLMKIFALRYHYFIEPWNLFDFVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKDKSGLDDVYNFKTFGQSMILLFQMSTSAGWDGVLDGIINEEDCLPPDNDKGYPGNCGSSTIGITYLLAYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPDGTQYVRYDQLSDFLDVLEPPLQIHKPNRYKIISMDIPICRGDMMFCVDILDALTKDFFARKGNPIEETAELGEVQQRPDEVGYEPVSSTLWRQREEYCARLIQHAWKRYKQRHGGGTDASGDDLEIDACDNGCGGGNGNENDDSGDGATGSGDNGSQHGGGSISGGGGTPGGGKSKGIIGSTQANIGIVDSNISPKESPDSIGDPQGRQTAVLVESDGFVTKNGHRVVIHSRSPSITSRTADV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VJZ5</accession>
    <name>A0A6E8VJZ5_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Endophilin-A</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the endophilin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VJZ5"/>
    <dbReference type="EnsemblMetazoa" id="ACON004766-RC">
        <property type="protein sequence ID" value="ACON004766-PC"/>
        <property type="gene ID" value="ACON004766"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012564"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004766"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_043205"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016191">
        <property type="term" value="P:synaptic vesicle uncoating"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004148">
        <property type="entry name" value="BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050384">
        <property type="entry name" value="Endophilin_SH3RF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14167:SF81">
        <property type="entry name" value="ENDOPHILIN-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14167">
        <property type="entry name" value="SH3 DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03114">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51021">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BAR" evidence="6">
        <location>
            <begin position="1"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="150"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="159"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="180"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004766-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="193" mass="21840" checksum="1CCADAEFC29C6832" modified="2020-08-12" version="1">MGDSLKQMADVKYSLDDNIKQNFLEPLHQLQTKDLKEVMHHRKKLQGRRLDFDCKKRRQAKDDEIRSAEEKFAESLQLAQVGMYNLLENDVEQVSQLVTFAEGMLDYHSQCSEILKVLVETLNEKREEAAARPKSEFVPKTLADLNIEMLGSQDGMNGGSSSHLSSPVNKPTHLELLGNGQAQARSTNGKRNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VJZ6</accession>
    <name>A0A6E8VJZ6_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Rho GTPase-activating protein 39</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON004524-RA">
        <property type="protein sequence ID" value="ACON004524-PA"/>
        <property type="gene ID" value="ACON004524"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012990"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004524"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_029473"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04389">
        <property type="entry name" value="RhoGAP_KIAA1688"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000022">
        <property type="entry name" value="Rho GTPase activating protein 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.555.10:FF:000011">
        <property type="entry name" value="Rho GTPase-activating protein 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.530:FF:000007">
        <property type="entry name" value="Rho GTPase-activating protein 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.530">
        <property type="entry name" value="MyTH4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000857">
        <property type="entry name" value="MyTH4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038185">
        <property type="entry name" value="MyTH4_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45876">
        <property type="entry name" value="FI04035P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45876:SF8">
        <property type="entry name" value="FI04035P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00784">
        <property type="entry name" value="MyTH4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00139">
        <property type="entry name" value="MyTH4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51016">
        <property type="entry name" value="MYTH4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="2" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="WW" evidence="9">
        <location>
            <begin position="44"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="MyTH4" evidence="11">
        <location>
            <begin position="934"/>
            <end position="1102"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="10">
        <location>
            <begin position="1113"/>
            <end position="1302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="92"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="140"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="270"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="355"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="602"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="695"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="104"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="162"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="182"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="208"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="271"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="297"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="357"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="383"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="439"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="487"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="519"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="557"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="602"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="696"/>
            <end position="727"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00070269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004524-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1307" mass="141786" checksum="388973388023D90D" modified="2023-06-28" version="2">MSTSSERVEWVEIIEPRTKEHMYANLTTGECVWDPPEGVTIKRTDASQWWELFDTNTCRFYYYNVASQKTVWHRPQNCDIIPLAKLQTLKQNTDPSDRKDATLPHHHHHHHHHGTGSSGSTAGGIGGLGSLGGASKLGGSGTVGKSCGHRTATGVTDYRDRDHHHRSSSSGHRRGGGGGLDQRSNSELLASPQGRHSLQHSYRMLQESSSSHNIPHSVVVPSPSSSTAAVSAVSSSEKLALGALHHSSALPPPHQMLSYSISNSSDLAAGSLSSPSHSLSTPQFKKKFMHDGGVGGSSSKSTSKDGNLAGGMVSKHESFDYGGNPSASSISLTRSGSFLSSAGITSGVGGVLGTPISSRPYGPSGSGGSHHSSSGSHRKGSFDGNGGSGNVPGSGAGGNVGGSGTAAGGGGGGSDDSMHEKYFKSVENTPITRRRHTTSSKSPLQQQKQSSDSSPQSPISPQQQQQVKVQRPSALSVELSPGSSYQNNGSNKSSHKNSNTNSSNTTGDHPSEPYSVDGLKGKADKYQEKMKQDRAGKISPGAVSVGGKQSPYPPNIPASSSSSHPADQTNSGSTSKGSAGKDHFSLNLNQSNLDRHNRQLDPQQTLEKKSSGSTHTVASNNSSSHNAKHGKKSKNYSNHNTAGGTGFDFDYDNGNTSPLYCNWDKEMQEHLLPLQHYILEQAKLSGSYGLGDLDSDSFHSDSQSEHSFSGHEPDNEDSDHSDGHGDYLAHYPYEEYGARHAQDGGVSYYNFEFNFGDRDEKEDISEEVTAKLESMPDQIYSPVKNHPPLHKFPPAAFQGARSALDASGLVPDAVDRGVPTVPMGGANSTFGSKTSYLQQMAAAAGHHQQLQLGGAMQSPQPQPPHKINKATLLQRFQENLSLGASAPGVGGNGKIAEMALLKECDIEKFAQDNLNLHSKGIFRKKSSVRDMLSWTSNAISRPMLSLARDKAGKKMATDLFKLVQIYMGDRKARIGMSLNSVAIDIVTMAMGQAQLRDELYIQMCRQTTENPSRDSLIRGWELMAICLSFVPPSPTFQPALLGYINRHRDPSFATSFPEVGKWPIHVQISHYATIACRRLDRIGSSGRKQAKRPTEDEINQAREQIFRDSMFGNTLSEVMELQKERFPDRQLPWIQTTLSEQVLLLNGKQTEGIFRVPADVDEVNMLKNLIDRWEFPENKGTMDAHAPASLLKLWYRELYDPLIPDELYDECVQTEDPAEAAAIVEKLPKINRLVLTYLIHFLQQFSLPDVVANTKMDSSNLAMVFAPNLLRCQSQDPKVILENARKEMTFMRTLIQHMDTSSVTYLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VJZ8</accession>
    <name>A0A6E8VJZ8_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ras-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_040219283.2">
        <property type="nucleotide sequence ID" value="XM_040363349.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040219285.2">
        <property type="nucleotide sequence ID" value="XM_040363351.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040219286.2">
        <property type="nucleotide sequence ID" value="XM_040363352.2"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON004692-RA">
        <property type="protein sequence ID" value="ACON004692-PA"/>
        <property type="gene ID" value="ACON004692"/>
    </dbReference>
    <dbReference type="GeneID" id="120947736"/>
    <dbReference type="KEGG" id="acoz:120947736"/>
    <dbReference type="CTD" id="32754"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000481"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007480"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004692"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038199"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04013">
        <property type="entry name" value="C2_SynGAP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13262">
        <property type="entry name" value="PH_RasSynGAP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05136">
        <property type="entry name" value="RasGAP_DAB2IP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.506.10">
        <property type="entry name" value="GTPase Activation - p120gap, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021887">
        <property type="entry name" value="DAB2P_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039360">
        <property type="entry name" value="Ras_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023152">
        <property type="entry name" value="RasGAP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001936">
        <property type="entry name" value="RasGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057606">
        <property type="entry name" value="SynGAP1-like_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10194:SF60">
        <property type="entry name" value="RAS GTPASE-ACTIVATING PROTEIN RASKOL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10194">
        <property type="entry name" value="RAS GTPASE-ACTIVATING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12004">
        <property type="entry name" value="DAB2P_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25321">
        <property type="entry name" value="PH_RASGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00616">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00323">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00509">
        <property type="entry name" value="RAS_GTPASE_ACTIV_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50018">
        <property type="entry name" value="RAS_GTPASE_ACTIV_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="4">
        <location>
            <begin position="110"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GAP" evidence="5">
        <location>
            <begin position="293"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="960"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1123"/>
            <end position="1150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1245"/>
            <end position="1301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1404"/>
            <end position="1426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="960"/>
            <end position="971"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="972"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1261"/>
            <end position="1282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1406"/>
            <end position="1417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004692-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1426" mass="160128" checksum="D4F1ECDF133116B7" modified="2020-08-12" version="1">MGQKGESTSRFTNFFSKRSFRSIPLKRTKSVIKLERSKRGQGGIRGSRSHESLLSTHVVMSNIDLACIGTIEVVPVHSSVLGRRHCFQVRGISHEERYYSCGTRQERDIWIHSLRKSISPNKDNRRRLDNSLKMWVYEAKSLPPKKRYFCEIYIDKTLYGRTSVKLRADLLFWGEYFDFPDIPEASIITVNVYREAEKKKKKDKHVLVGSVVIPIEKVAARTFSEDWYQILMEKQDNVIKSPSKEPAPTLRIKCRYQSIDIMPLDVYGEFHEFLKKNYKKICEVIEPIIGVKAKEDIGQALVLQMHSQGMAAIFLSDVVALDLLRVDDQRLTFRGNSLATKSMEAFLKLIGEQYLQDTLSIPIAEIIASDRDCEVDPTKVNGSLSRQQQALRRTVKSVWIAIAESSKNFPKQLRDCFATFRERLHDLDREDMADNLISASIFLRFLCPAIMSPSLFNITNELPSARATRNLTLVAKTLQTLANFTRFQGKENFMEFLNDFLEQEAPRMKQFLYDISCSNFSNPDNFMDWSGCIDQGKQLSILHSLLYEIVLKLPADKQHEIQPLPAILATITHCKQVNCTTLNADQYCTGKPHIVQVKENLLSKKEDILQSAGSANMSSKEYSTKRNPKILKNECTVQSSNTLQPSTHSSSFSLHYSTSTSTNSLVNNTKQLDKDATDKNDDICAMGKLNFNSQYGSNIICSGSLRTYSSTGATSFGAVNSLMTNSLRNEKEKSNNLQGDIRANTLPRYNNYTTSVSIRENTYPESSNSVGYDHEVNGNSRLNGINKNLIQIDIDPTNPINRKSPTPLLKNISCCHYIANNRIQEGSHQRLTSPGSNLSDLDKNAFNLGIPHNDTNREQQYSQHIYPAPALKASNITTISSFVPRQSTTTFNPSKMPMSLEDLEDLLNYADEQNQTSFSESVSNRGGCDPCGTNTKANIASNGSNASISQISNICSSGYQSIPTQSTSSSPVESTGEQHSLVTNRAPPSRKLTTTLIGYNIGTSQKLCMNDQAIAKADLPVSSINMQLSNKSDPLVTSHGTIHHYYDRKTPNEQSNVNRILNNNYVDISYDVLSPCKSPIASKKTECTSLGIDEHNDLAHPVEYFGINRTNHHSRHKRKVIHGIRRSGSSIQSESSDDERVSNTTSENFNDIDSLTTGNVSALERNHILISNGNDYDQASSGLFGCRLGCRRMPRTNPLMQYDKDDVSDIIGNSGVDTALTNHQRNQSFQSRFHRRLSIECARALSDSSTDETESEKTKTFNSNQHTAQHHNLSISTTTHTNIFHENDTRRRRNANKSVEQCEREIQRLQASLDSMRKKLEMSEPAEGNANKLAAITQQSDSKIRSIIGRLLTMEEELRQEQYKMSLVLSHKQRVIEAQGKQIAALDAANNRLLSALSTLQNRYESQSNQSDDTSSSHPNAGASSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VMC2</accession>
    <name>A0A6E8VMC2_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="11">BTB domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Putative transcription factor required for axon growth and guidance in the central and peripheral nervous systems. Repels CNS axons away from the midline by promoting the expression of the midline repellent sli and its receptor robo.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VMC2"/>
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        <property type="component" value="Unplaced"/>
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    </dbReference>
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        <property type="entry name" value="Lola, isoform F"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110:SF111">
        <property type="entry name" value="LONGITUDINALS LACKING PROTEIN, ISOFORMS F_I_K_T"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="3" id="KW-0221">Differentiation</keyword>
    <keyword evidence="4" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
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        <location>
            <begin position="32"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="115"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="301"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="458"/>
            <end position="500"/>
        </location>
    </feature>
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            <begin position="161"/>
            <end position="170"/>
        </location>
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            <begin position="175"/>
            <end position="186"/>
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            <begin position="212"/>
            <end position="221"/>
        </location>
    </feature>
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            <begin position="322"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
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            <begin position="477"/>
            <end position="492"/>
        </location>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="653" mass="68810" checksum="62A7759AF6A1B155" modified="2020-08-12" version="1">MDDDQQFCLRWNNHQSTLISVFDTLLENGTLVDCTLAAEGKLLKAHKVVLSACSPYFATILSQQYDKHPIFILKDVKFQELRAMMDYMYRGEVNISQDQLAALLKAAESLQIKGLSDNRSSSSSGTAAAGGGVHQKQSDAPAAKTLQPPVPASKASGLTIENKRSLKSELLESDVSGSREGSTSPTSRKRKKVRRRSVDTNNLLIDNHDQHSNSSSHSMHTAGGANVAAAVAAAGAPAANVASSLSSASSTAAAGSNTSANVLSVAAVAAAAVLKKTETVQQAAAETLKLQKQQAAAAAAAAAAQSSDDEERCGPGSVATGTEHDDGNDLSDNEHDGDGGSSVKSGSRHHHLRDGSRRGKTSTSSMGEMMIEPKSEYEDVQDENVEDLTMDDEDLMDELDQAGPSHGGDVSNAVAGMMLSAFNRHHHHDRSDSDEPIAMAAAAAAPSEMLLLSLSTSPSLVQQEQHQPEYPPSQQHSLSSSVAFDGGSSSLSALSPKTASNCAVRGDRDRVLADHPLKIRSIMSNLGGTMRKSDFISARNESCVTISARSSLAAAACNLPDSTAQELLRHEREPRGSPRFVSSSSSSRITVATGLLLQSSTSLAAGNCGSGGGGAGTAQGKRFRCDYCTFSCSWRYDLKLHLKQKHGVHKKNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VMC3</accession>
    <name>A0A6E8VMC3_ANOCL</name>
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        <recommendedName>
            <fullName evidence="1">WD repeat-containing protein 89</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VMC3"/>
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        <property type="protein sequence ID" value="ACON005524-PB"/>
        <property type="gene ID" value="ACON005524"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009835"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
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        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
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        <property type="entry name" value="WDR89"/>
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        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 89"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22889:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 89"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="WD40 repeat-like"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0853">WD repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005524-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="257" mass="28834" checksum="91E2EB31192BA5A6" modified="2020-08-12" version="1">MTSCDINQNDRVLCASSEVQKTGDSFLLFFDVRERNYLGCYWECHSDDITHVRFHPTNPDLFASASVDGLINVFDISKLTEDDAMQYCFNVETAVDSINWHADPTGRDLVSCVTTTNDLHLYDVESQDSVALFDRAQITKSLRRTSAIDCNVVGTHNYGNGSFFVLAGSNFNRGKNCLRTLRYDNKTLLPDRSFGGNKQIVRASVYNEKDQYLIATGECGLITLWKCRTDGSEQTGVNGASKSLKQKLHVSHRAKPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VQG6</accession>
    <name>A0A6E8VQG6_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the troponin C family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VQG6"/>
    <dbReference type="EnsemblMetazoa" id="ACON006178-RA">
        <property type="protein sequence ID" value="ACON006178-PA"/>
        <property type="gene ID" value="ACON006178"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006741"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON006178"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033130"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000103">
        <property type="entry name" value="Troponin C Ib"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF0">
        <property type="entry name" value="CALMODULIN LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="7"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="43"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="84"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="120"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON006178-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="151" mass="17275" checksum="90F280AD66A75B68" modified="2020-08-12" version="1">MEELSKDQLKVLRDAFNAFDKEKTGSIPTDVVGTILELLGHKLSEEELDEVIEEYDEDESGQLEFEEFVALASNYVEPEEDYEALRKELREVFMMYDKDAKGYLPVEEFKAILRELDGAVPEEELDDIVDEIDADGSGTVDFEEFMEVMTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VQH4</accession>
    <name>A0A6E8VQH4_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="13">C2H2-type domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040223603.1">
        <property type="nucleotide sequence ID" value="XM_040367669.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VQH4"/>
    <dbReference type="EnsemblMetazoa" id="ACON006701-RB">
        <property type="protein sequence ID" value="ACON006701-PB"/>
        <property type="gene ID" value="ACON006701"/>
    </dbReference>
    <dbReference type="GeneID" id="120949988"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005746"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON006701"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040600"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000621">
        <property type="entry name" value="FLT3-interacting zinc finger 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001506">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000670">
        <property type="entry name" value="zinc finger protein 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001498">
        <property type="entry name" value="Zinc finger protein 404"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000624">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF142">
        <property type="entry name" value="ZINC FINGER PROTEIN 384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="20"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="48"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="76"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="104"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="136"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="164"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON006701-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="223" mass="25493" checksum="CE4DC3D113E4BC5C" modified="2020-08-12" version="1">MSQQAVESGGMKISNCSKPFACTECNKLFRQLSTLTNHMKIHTGEKPYKCTICMKEFRQTTTLSNHMKIHTGEKPFNCNFCDKQFRQLSTLSNHKKIHTGEKPFECSVCGKQFRQSSTLNSHIRIHSDDKFCEKPYACTYCSKTFRQTGTLSNHLKIHTGEKPFECSVCGKQFRQSSTLNSHIRIHADDKYCKPSQTEPRSMVALNGGLNLKDEDVKPFFAMM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VQI4</accession>
    <name>A0A6E8VQI4_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Gp150</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VQI4"/>
    <dbReference type="EnsemblMetazoa" id="ACON006645-RD">
        <property type="protein sequence ID" value="ACON006645-PD"/>
        <property type="gene ID" value="ACON006645"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010173"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON006645"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040555"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050333">
        <property type="entry name" value="SLRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45712">
        <property type="entry name" value="AGAP008170-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45712:SF22">
        <property type="entry name" value="INSULIN-LIKE GROWTH FACTOR-BINDING PROTEIN COMPLEX ACID LABILE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026001160" description="Gp150" evidence="5">
        <location>
            <begin position="21"/>
            <end position="1103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1033"/>
            <end position="1055"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="39"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="144"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="880"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="921"/>
            <end position="1012"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="168"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="230"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="291"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="306"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="885"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="895"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="936"/>
            <end position="977"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1002"/>
            <end position="1012"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON006645-PD"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1103" mass="124419" checksum="77855882E8ED4DCB" modified="2020-08-12" version="1">MRCQYLLVLTVVLCALTVNSLKPDLPKAKDPLFNKMLAESSTKGVDAQDPRYNKMLPDLEENLIDDDDEDDDEEEEDDVAPPTKKAADLNPMYALRGKQVKPEVSTVGSVAINKVKINEDIDSYEEVLLKGNGKSVDIDDSYQVEDLSGEKPSKSTTAKPVAKPATEDNYDEYDDDESDEQIDFSGVDKVLAQPLKPQVPAEKSAKPAVPSTTAKPKAVTTGKQNVEILDQYDDDADMDYQYNYDDDEDNDDDDDEDDDDDEEEDAETLPAQKITNKVNKEVAKKQTNPVKETKDQNKAAGKDNAEDYYDDDEYDDDDNAEPSNSSYCPRGCICERNMHAYMVATCSRLDLETQKFTSHITDLQVLDVGPKYPIELGPEFFKKIGLSHVVSIKITNCTIVYISPQAFAGLDELYSVNLTNSGIDIIHPDTFANNTKLRLLTLSGNDLSAMQSVNHNTPYMDYMLKAPTVEELDISRCKLQELQPNAFNELKNIIYINLSENNLSNLPEGIFDNVETIEELDLSMNNIVELPKNIFAKTSLAILHLKHNKITNNVDFVTADLQKLDLSFCQIRTVHNTMFKGMDGLTNLILKGNHIEKIKPMAFISLKNLRQIDLSYNNLEQISAQTFIGNKMLDIIRLNNNPRLKRLPNEGFEISFNGTFTVYFMDVSNCDISELADNTFKMMPHLTRLNLAWNNLQTIRSTYFAHLNKLMDLDLSNNMIINMDEKTFQNNRNLNTLNLAGNQIATLTSKLFHPLQYLSELDVSDCDLRTIWDDSAAGTKREEVLPNLKRLNVSYNEIMEVFVSDLESMGKLRVLDIRNNTLTCNERLPTLIDWLQKKQISMGLGDTHDHVTHAEMTTELRTDNSATLKQWGQFAWEICQGGGSDSPNQRLTYSESDEEYNIEDEDDVSDAADADVMAEAKNAKSNKIVDSKNELDEYSDDSVDSDDSEDEDSADEDDEEDDEEDDDDDDDDEEEQERENILDAANGIQKIEQGILTGKQTDEDDDGEYEDDGASVDDVVILENGSMFAVSSVWIVVACILFGISMLLLVVVKVLTEVMKRRGERYRQALLASKNSFVYQKLTEDIVPSKVPKIHRYEPINQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VQI6</accession>
    <name>A0A6E8VQI6_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Aminotran_1_2 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VQI6"/>
    <dbReference type="EnsemblMetazoa" id="ACON006541-RB">
        <property type="protein sequence ID" value="ACON006541-PB"/>
        <property type="gene ID" value="ACON006541"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002951"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON006541"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033449"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047536">
        <property type="term" value="F:2-aminoadipate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000080">
        <property type="entry name" value="Aminotransferase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42858">
        <property type="entry name" value="AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42858:SF1">
        <property type="entry name" value="LD15494P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="1">
        <location>
            <begin position="56"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON006541-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="427" mass="48127" checksum="C46CA6EC2D75FD8C" modified="2020-08-12" version="1">MDSVRQEISQRYNFDLPTMDVYRTEDTAIMGMGLPPEKLLRECCYIFQQATAKRMKYELKNSSFLFQYGPSIGTTEFRKSLASFLADGYRSEVNMSDLVQTSGATNGLHLILSTLVDLAGVIFVDEYTYMIALETIAQFNTMKIVPVPLDEDGPNIATLQALIDQHRFEPTKGKLFWGVYYTMPTFHNPTGIVFSEAKCQQLIKLARATDILIACDDVYNLLHYADPDAPPPKRLFARDIADLGRVEGWHGNVISNGSFSKILSPGIRLGWMECPPRCVEAFRNSGVLMSGGAANNYTGGVVATLIQMGLARRQLDHYRTNYAARLDAMAETLQAHLPAGCSFRKPNGGYFVWIRFPSHIDCAEFNRYCLEKYRVSAIPGVRFSFAKEARHFLRLTFAFHEPDYLRRSVRTLCEAAREYLEIAASTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VSI5</accession>
    <name>A0A6E8VSI5_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="4">SAM domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VSI5"/>
    <dbReference type="EnsemblMetazoa" id="ACON007137-RC">
        <property type="protein sequence ID" value="ACON007137-PC"/>
        <property type="gene ID" value="ACON007137"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001949"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007137"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038657"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd09564">
        <property type="entry name" value="SAM_kazrin_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09567">
        <property type="entry name" value="SAM_kazrin_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09570">
        <property type="entry name" value="SAM_kazrin_repeat3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037614">
        <property type="entry name" value="Kazrin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059089">
        <property type="entry name" value="Kazrin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037613">
        <property type="entry name" value="Kazrin_SAM_rpt_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037615">
        <property type="entry name" value="Kazrin_SAM_rpt_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037616">
        <property type="entry name" value="Kazrin_SAM_rpt_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12776:SF1">
        <property type="entry name" value="KAZRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12776">
        <property type="entry name" value="KAZRIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25986">
        <property type="entry name" value="Kazrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07647">
        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAM" evidence="3">
        <location>
            <begin position="623"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="3">
        <location>
            <begin position="706"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="417"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="536"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="901"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="252"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="37"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="105"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="131"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="168"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="932"/>
            <end position="942"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007137-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1044" mass="111318" checksum="6F0747AEA871A754" modified="2020-08-12" version="1">MQLNGTAGTVTMAAASEPPEPPPRNPDKINASLKQLAESKTRSLDAADKAGASTTTNGGRPFRPILSLDNAKPSTKTFDIPERGTPTSAPATAFGTDHQPGGPEPGTSTSGNSSSSAAGTTQRQSNQPTLSGSSSSSSSGEAATANGTATARETAVPNASSASTTTSPQDKASGQAKQISPTLAASEPGGDNLFKNGVSDLESPQTTKIRLENERLSAEITRLKRLLENSPDGAVGGIDLLSQSNSFEVHAADDSSNRMEKLENELRISKELIQSLKLERKKLRADKNDLLSQVKQLCSSLQDKEQELRNFIRNYEQRIRESESNSAKQSTERERERWSLLKHARDEAERSIALAAQLNARDLQLQRTQEQLQEARRQLSGCMSDQESLVSAAPLTPPSGLMAHNAVAPASTPGVGYSNISGTPGDRGSCSADSGVRDFVAGSSDRESAAGDLNLSDGPCENGPCIQVDSDSVSLVSSHHNVYQYGTPKERSPTLSPLNAGAYSRSVDSAALSSSYRSVEQLGSPVDNENQTFARRLQQQQSASGAVPAASGKAQNTGLTGRTGRGGTWGSISRVFARSRNRNKNAPIDTEATDFQWSPLTEEGYAEKLRLLREASSMPMERWRASQVLAWLEVALGMPQYSGRCAENVKSGKVLLELSDAELETGLGTTHPMHRKKLRLAIEEQRRPELVRYPTIGQLGHTWVASEWLPDIGLPQYGESFMHSLVDARMLDTLSKKELEKYLGVTRKFHQASIVHGIHVLRIMKYDRQAIAMRRLQSENVDTDPIVWTNQRFIRWARSIDLGEYADNLKDSGVHGGLVVLEPSFSGDTMATALGIPPSKNIIRRHLATEFEALILPARTTMGQGIRTGGGAVIPIASGIGMMAGAAASDRRNTGSLRVNGWRESHPSSSHSRVLVAGGGSGAGSDKNSVYSSTSPTPSFSSSEGGIYATVGSLQSHPAFFHHLPPPTSAPPPIPHGTGPAGGLRSIDPMLVGPGGTATAATIANNRVSAPPMDSGHATLPKYAQHRRVKSISDIEIPSVGPVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VSJ6</accession>
    <name>A0A6E8VSJ6_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Rhodanese domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VSJ6"/>
    <dbReference type="EnsemblMetazoa" id="ACON007534-RA">
        <property type="protein sequence ID" value="ACON007534-PA"/>
        <property type="gene ID" value="ACON007534"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002169"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007534"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030127"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd01519">
        <property type="entry name" value="RHOD_HSP67B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026235109" evidence="1">
        <location>
            <begin position="24"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodanese" evidence="2">
        <location>
            <begin position="54"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007534-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="155" mass="16879" checksum="D5301ACD88CD5D20" modified="2020-08-12" version="1">MAPIVWLFSRCTVVLLLVSVTAAAPNDCGGNRFAPACIEPQLVATTADVDDLPNHPEKLLIDVREPSELAATGQIPTSINIPLKTVRTELSLSPEEFEKKYGRKKPATNDPIIFSCRSGVRAGQASFEADQLGFKNVKNYVGSWLEYAAKHDLPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VSK0</accession>
    <name>A0A6E8VSK0_ANOCL</name>
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        <submittedName>
            <fullName evidence="4">Homeobox domain-containing protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
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            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">Ngousso</strain>
        </source>
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        <property type="gene ID" value="ACON007539"/>
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        <property type="component" value="Unplaced"/>
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    </dbReference>
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            <end position="213"/>
        </location>
    </feature>
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            <begin position="16"/>
            <end position="82"/>
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    </feature>
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            <begin position="121"/>
            <end position="143"/>
        </location>
    </feature>
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        <location>
            <begin position="20"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="32"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="54"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="123"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF16493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007539-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="273" mass="28540" checksum="F87A4C1ADC458420" modified="2020-08-12" version="1">MQENSVTAVPVSAVTASGLGTIGTTGPGSAGVVGSAVNSTSGSSNGSSSGGTTGTSNNGPGSGPITTASTGPASGSTLSPHGLSDIDQAQFEADKRAVYKHPLFSLLALLLEKCEQATQGYIPSSSSASSPNGSSNHGPSDGDSFSRDIQAFVQLLEKEKRPLLTNNSELDGLMIKALQVLRIHLLELEKVQELCRDFCTRYIACLRSKMQSENLLRSDYALEHNNNLSNSNSPINSPEQDLSGSAQGYYQSADYLQGSDTSEYSNMQASLSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VSL2</accession>
    <name>A0A6E8VSL2_ANOCL</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
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            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
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    <dbReference type="EnsemblMetazoa" id="ACON007312-RA">
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        <property type="gene ID" value="ACON007312"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000090"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007312"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_036408"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="108"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007312-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="141" mass="16918" checksum="7F3BC3F1A29BE692" modified="2020-08-12" version="1">MERYLRFYIKFQGYMIGGFTLLLSILMTLVIFESNDPYYPMEFYYNYRFMGTTALVLGIFWFIAGVTFMYGVHYEIKQCLLPFALLYLVDLALLAIRDIVMIWHDRRWYTMVFVNIPTLLAVLCKCCLPRRTASPFKRHHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VSL7</accession>
    <name>A0A6E8VSL7_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">3',5'-cyclic-AMP phosphodiesterase</fullName>
            <ecNumber evidence="4">3.1.4.53</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">Ngousso</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cyclic nucleotide phosphodiesterase family. PDE8 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.53" evidence="4"/>
    <dbReference type="RefSeq" id="XP_040222771.1">
        <property type="nucleotide sequence ID" value="XM_040366837.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VSL7"/>
    <dbReference type="EnsemblMetazoa" id="ACON007163-RA">
        <property type="protein sequence ID" value="ACON007163-PA"/>
        <property type="gene ID" value="ACON007163"/>
    </dbReference>
    <dbReference type="GeneID" id="120949498"/>
    <dbReference type="CTD" id="37741"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_008700"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007163"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035887"/>
    <dbReference type="UniPathway" id="UPA00762">
        <property type="reaction ID" value="UER00747"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004115">
        <property type="term" value="F:3',5'-cyclic-AMP phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006198">
        <property type="term" value="P:cAMP catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000096">
        <property type="entry name" value="High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1300.10:FF:000002">
        <property type="entry name" value="Phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1300.10">
        <property type="entry name" value="3'5'-cyclic nucleotide phosphodiesterase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057304">
        <property type="entry name" value="PDE8-like_REC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023088">
        <property type="entry name" value="PDEase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002073">
        <property type="entry name" value="PDEase_catalytic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036971">
        <property type="entry name" value="PDEase_catalytic_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11347">
        <property type="entry name" value="CYCLIC NUCLEOTIDE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23198">
        <property type="entry name" value="PDE8A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00233">
        <property type="entry name" value="PDEase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00387">
        <property type="entry name" value="PDIESTERASE1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51845">
        <property type="entry name" value="PDEASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0114">cAMP</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PAS" evidence="12">
        <location>
            <begin position="281"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="PDEase" evidence="13">
        <location>
            <begin position="542"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="71"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="8">
        <location>
            <position position="618"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="618"/>
            <end position="622"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="622"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="660"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
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        <location>
            <position position="661"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
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        <location>
            <position position="661"/>
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
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    </feature>
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        <location>
            <position position="661"/>
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
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        <location>
            <position position="778"/>
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        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
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            <position position="778"/>
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        <ligand>
            <name>Zn(2+)</name>
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            <label>1</label>
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        <location>
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        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004703"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006437"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00012276"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-1"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007163-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="900" mass="101658" checksum="0CC8417F9BF8D9CD" modified="2020-08-12" version="1">MDISKVLIRQLSAREWRRFVAFRSLPADSIDPAFRQQILADYDVHFYEYGELAASSAQEVVSADTVTFAVGEPTAGSSRPQQRWPEGSDGKQRQLVESVVESASQEDVQDTILPTLSEEEAIFIERLVSDLKFPNLLPPNPVIKILIAFCKNDPVFEALVNASHRLSLEPTFVKSAESAIDAFQNPSSGGHHIIIVDARYPRTIEAEALGRSIRNSKGSQHTTMVAVVKKSVFEKDDTAVISLLDVGYNRCIIESPHVSICSSELRQIQHSLVRPQNVISTQQALYTALHRSREAVIITDDTLRAQYANRASERVLNMKLDEIVGRSLSDLVTADISNILSHTSRYKDYDGYLTTRRKSQESSQIHVRAVPVSCIGRNPTHLVLILESSSSALVSTNAIGEALQTLPQGKEVPRGSLHSIRRGSFDVRSIASDGLRRTSLAKLSSLPLEAPITKVLSLLAQVQENCSLDEAKLLDRVMEFLKREGLYSPQMKEIRTEDPVATDLIGALLTQGPTNILSSRRSSNDSIIRGGGRPSTGSIVFNKTKESSQLSDLLQTALDWDFEIFKLEDLTEKRPLVCLGLELFRRFDVYNTFNCDEMTFKLWLIEMEKHYHSENTYHNSTHAADVMQATAVYLQQLSNRELKIMDRMDEATALIAAATHDIDHPGRSSAFLCNSDNTLALLYNDICVLESHHAATTFRLTLGDDKINIFKHLDRDMYKLARSIIVDMILATEMTRHFEHLAKFVSVFGTDVESKDPLPPDNDDNQILVRRMLIKCADVSNPTRPLKFCVEWARRIAEEYFMQTDEEKRKNLPIVMPMFDRTTCSISKSQIGFIEYIIHDMMDAWNSFIEMPEIIRYMEFNYSQWKLFEEQGINTLSDIKRKQMSLTEDTASSISLEEKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VSM3</accession>
    <name>A0A6E8VSM3_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Titin</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Myofibril</location>
            <location evidence="1">Sarcomere</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_049463023.1">
        <property type="nucleotide sequence ID" value="XM_049607066.1"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON007563-RA">
        <property type="protein sequence ID" value="ACON007563-PA"/>
        <property type="gene ID" value="ACON007563"/>
    </dbReference>
    <dbReference type="GeneID" id="120948117"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_013821"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007563"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039286"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031674">
        <property type="term" value="C:I band"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000147">
        <property type="entry name" value="Myosin light chain kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000107">
        <property type="entry name" value="Myosin, light chain kinase a"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000032">
        <property type="entry name" value="palladin isoform X1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000119">
        <property type="entry name" value="Sallimus, isoform P"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000430">
        <property type="entry name" value="Sallimus, isoform P"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Sallimus, isoform P"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Sallimus, isoform P"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Sallimus, isoform P"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Sallimus, isoform P"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Sallimus, isoform Q"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Sallimus, isoform U"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="35"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47633">
        <property type="entry name" value="IMMUNOGLOBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47633:SF4">
        <property type="entry name" value="MYOPALLADIN ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="35"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
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        <property type="match status" value="35"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="31"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="35"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="35"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="10" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="163"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007563-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="4895" mass="550491" checksum="1E82A031E75197C4" modified="2020-08-12" version="1">[encoded data, 4895 characters]</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VSM8</accession>
    <name>A0A6E8VSM8_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">DnaJ homolog l(2)tid, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Protein lethal(2)tumorous imaginal discs</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_049463030.1">
        <property type="nucleotide sequence ID" value="XM_049607073.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VSM8"/>
    <dbReference type="EnsemblMetazoa" id="ACON007565-RB">
        <property type="protein sequence ID" value="ACON007565-PB"/>
        <property type="gene ID" value="ACON007565"/>
    </dbReference>
    <dbReference type="GeneID" id="120949526"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_003057"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007565"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_029367"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031072">
        <property type="term" value="F:heat shock protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10747">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10719">
        <property type="entry name" value="DnaJ_zf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.260.20:FF:000005">
        <property type="entry name" value="Chaperone protein dnaJ 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.230.10:FF:000003">
        <property type="entry name" value="dnaJ homolog subfamily A member 3, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000075">
        <property type="entry name" value="Uncharacterized protein, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.230.10">
        <property type="entry name" value="Heat shock protein DnaJ, cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.260.20">
        <property type="entry name" value="Urease metallochaperone UreE, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01152">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051938">
        <property type="entry name" value="Apopto_cytoskel_mod"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012724">
        <property type="entry name" value="DnaJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002939">
        <property type="entry name" value="DnaJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008971">
        <property type="entry name" value="HSP40/DnaJ_pept-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001305">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036410">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44145">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY A MEMBER 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44145:SF3">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY A MEMBER 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01556">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00684">
        <property type="entry name" value="DnaJ_CXXCXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57938">
        <property type="entry name" value="DnaJ/Hsp40 cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49493">
        <property type="entry name" value="HSP40/DnaJ peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51188">
        <property type="entry name" value="ZF_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="13" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="8 16" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 16" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="J" evidence="18">
        <location>
            <begin position="105"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="CR-type" evidence="19">
        <location>
            <begin position="249"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="CR-type" evidence="16">
        <location>
            <begin position="249"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="64"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="493"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="64"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="511"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00080150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00093620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007565-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="525" mass="57111" checksum="2D52D46412AB6D1A" modified="2020-08-12" version="1">MGSRGILQIFSPKSFGLLNARFPGRSAPIGVAVPGVATVGACHCIITNQSPISRHLFTPATTSTTAAAASGSGPSRLQRPSPSSRCSTLQSTRSFYTSNILHKSDYYSTLGVTKNASPKEIKKAYYQLAKKYHPDTNKDDPNAGKKFQEVSEAYEVLSDETKRREYDTYGQTSEQMGRAGGGPGPGAGPQGFSQNWQFRSTIDPEELFRKIFGDGGFQTGFDDYSDSKFGFGGAQEVMMNLTFAQAARGVNKDIDVNVVDTCPKCTGSRCEPGTKPGKCQYCNGTGMETISTGPFVMRSTCRYCQGTRMYIKYPCLECGGKGQTVQRKRVTVPVPAGIEDGQTVRMNVGSKEIFITFRVEKSRYFRRDGADVHTDANISLSQAILGGTIRVQGVYEDQTIQITPGTSSHTRITLTGKGLKRVNSYGSGNHYVHLKIQIPTKLTPKQKALIQAYAELEDDTPGQIMGVTFKTDGGKVCYAEPQELTDIVREALKDRKPLPTSSAEEPAATPKTKEEEEAAKTRSSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VT01</accession>
    <name>A0A6E8VT01_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VT01"/>
    <dbReference type="EnsemblMetazoa" id="ACON007250-RA">
        <property type="protein sequence ID" value="ACON007250-PA"/>
        <property type="gene ID" value="ACON007250"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009293"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007250"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030283"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007250-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="180" mass="19802" checksum="F8725BD43300A6FB" modified="2020-08-12" version="1">VAIVTVQATLDRIRASKNLLSQLQSDDALESYRSETRSKLSEQTARKLADKMAMVNSDEDDFVSTGMTARKALKIRASTESSSVAKSSQALKWKDETAESYASKRASATKARLQDIDQEMEEFEAKQAARARRNAQLKQLLAETAAQDIVPASLSEHADGMKVTTGVLKVKTTQKKMVSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VT08</accession>
    <name>A0A6E8VT08_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="8">MFS domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VT08"/>
    <dbReference type="EnsemblMetazoa" id="ACON007253-RA">
        <property type="protein sequence ID" value="ACON007253-PA"/>
        <property type="gene ID" value="ACON007253"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007470"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007253"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038464"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17387">
        <property type="entry name" value="MFS_MFSD14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001958">
        <property type="entry name" value="Tet-R_TetA/multi-R_MdtG-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23504:SF1">
        <property type="entry name" value="GH21943P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23504">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01035">
        <property type="entry name" value="TCRTETA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="16"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="50"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="83"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="115"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="137"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="171"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="201"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="294"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="324"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="347"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="390"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="471"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="48"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007253-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="531" mass="57743" checksum="29B1C529B2E93749" modified="2020-08-12" version="1">MFFLLSYTLSLPSRKFSADCVSCFSHLLLFSFPFFPILFQSSGIGEPSVYHALVVIFLEFFAWGLLTMPVINVLNQTFPDHTFLMNGLVMGIKGILSFLSAPLIGALSDVWGRKFFLLITVFFTCAPIPLMSINSWWFFAMISISGVFAVTFSVVFAYVADVTTVEDRSRAYGLVSATFAASLVISPALGAYLNDKYSEPLIVALATAIAVLDVFFILVAVPESLPEKVRPSSWGAPISWEQADPFAALRKVGLDQTILMQCVTVLLSYLPEAGQYSCIFVYLKLKMHFSSIDVSIFIAVVGILSILTQVILGDLMKALGAKRTIIIGLLFEMLQLLWYGFGSQTWMMWAAGILASLASITYPAISAFVSIHSNPDQQGVVQGMVTGMRGLCNGLGPAMFGVIFYVFHVDLNDEHNVASHSLNGAAIGAVAGGGVFDGSDAKFVRNETLLGGSVHHHIEDEYSQLMPGPPFVFGALMVICAIAVAAFIPETPSDTMRRPSGLSLDVDYEYERGRKVAGPLSPLMHQDSAQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VT09</accession>
    <name>A0A6E8VT09_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">phosphatidate phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.4</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 1,2-diacyl-sn-glycero-3-phosphate + H2O = a 1,2-diacyl-sn-glycerol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:27429"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="EC" id="3.1.3.4"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:27430"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the lipin family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.4" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8VT09"/>
    <dbReference type="EnsemblMetazoa" id="ACON007636-RB">
        <property type="protein sequence ID" value="ACON007636-PB"/>
        <property type="gene ID" value="ACON007636"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011317"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007636"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_029681"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008195">
        <property type="term" value="F:phosphatidate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032869">
        <property type="term" value="P:cellular response to insulin stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009062">
        <property type="term" value="P:fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019432">
        <property type="term" value="P:triglyceride biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026058">
        <property type="entry name" value="LIPIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031703">
        <property type="entry name" value="Lipin_mid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007651">
        <property type="entry name" value="Lipin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013209">
        <property type="entry name" value="LNS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031315">
        <property type="entry name" value="LNS2/PITP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12181">
        <property type="entry name" value="LIPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12181:SF12">
        <property type="entry name" value="PHOSPHATIDATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16876">
        <property type="entry name" value="Lipin_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04571">
        <property type="entry name" value="Lipin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08235">
        <property type="entry name" value="LNS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00775">
        <property type="entry name" value="LNS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LNS2/PITP" evidence="7">
        <location>
            <begin position="931"/>
            <end position="1086"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="203"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="327"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="568"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="721"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="203"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="340"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="359"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="371"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="382"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="409"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="439"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="457"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="476"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="595"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="723"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="743"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="777"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="803"/>
            <end position="812"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007636-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1184" mass="128520" checksum="02EB88056066AADC" modified="2020-08-12" version="1">MNSIVNLFSNFKEFYSEINGATLTGAIDVVVVEQPDGSFVSSPFHVRFGKLGVLRSREKVVDIEVNGEPVDLQMKLGESGEAFFVEECAEDDSEVPAHMATSPIPSHSYLSNYEETVQQRNDGPGGPIFKALEANEATGAAGGPGAASVVGPTTIEVGEGGEVMKMVSSQTGAGGDGEEELLPRLRRNSVDLSNENAEVERAKFENQRSEYSHRRHTDNLTNRPDLSLTKKEYTTQKLRQELNAAAEAAEQEQIFHMEGLDDGGPLEGGVAGEEQQVMHAGEWKNSTPLPSGAGDHQLVEDKHFKPIDAQPTTGQATEIVPMVMATAQPSGDGGAADSKSKKKRRKKSLMKKKNTQRKNSASSSSGAGSNHSDPNEGTETDSLTASQVTVSSASGSSLPKEDAADGGDVDQQVPDVSSPLKKEGNDSPALGSTAMTSSHHADLDIHFFSDTDIGTGVSPQQSRPSTPIQSDTEFEISQREKPVSEEERLMYGNSWNWGELPTQVEPKAGDAEAGKQAQHNSMLTGLMSLMKYNNKKKRQSVPDGMYLSDLDKLTEPEIAQLYFPSFSKLKQQQQQHQGKRGSGGSGDDEDRESGNGTSIPQSPTSMEDGPGGKDSDFEERDQLLKSGAGGTVDRSIDSFALSLCGGLSGADDSAAGPTDEQFERHRLQYDNVLSDPSVFKSPDLVVRINGKYYSWEVACPQVMTILAFRCGLPNEFLASATKPKADDGLPVADNKKGEEQSLQQQTQPQPIQQPQPAGGSWWRWRRSNESKPPAPAGTPQTPITPTAAAEESPDGLDMKTSAAVGTQTSRSNSPDDESHEGGMDATVPTYKNASKTEDGYNGSLSSEDSELAMEKSMSAGGMLIDNSSEKTLFLLGCGEKYRKTLRLSSERIKELNLLDGMNEIEFSVTTAYQGTTRCKCYLFKWKHNDKVVISDIDGTITKSDVLGHILPMVGKSWDQIGVAQLFSKIEENGYKMLYLSARAIGQAKTTRGYLQSIRQGDVKLPDGPLLLNPTSLMSAFHREVIEKKPEQFKIACLNDIRDLFPERNPFYAGYGNRINDVWAYRAVGIPTSRIFTINPKGELKHELTQTFQSTYANMAYIVDQLYPPIKHIEEEDNEYTSFNYWREPVPEIDFNACDISGSSLSLSGAAAASATTTTYPAPIGGKITPTATLIQQALPTIPEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VUP4</accession>
    <name>A0A6E8VUP4_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Sushi domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VUP4"/>
    <dbReference type="EnsemblMetazoa" id="ACON007761-RA">
        <property type="protein sequence ID" value="ACON007761-PA"/>
        <property type="gene ID" value="ACON007761"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_003944"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007761"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030844"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030414">
        <property type="term" value="F:peptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000086">
        <property type="entry name" value="Hig-anchoring scaffold protein, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051277">
        <property type="entry name" value="SEZ6_CSMD_C4BPB_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008197">
        <property type="entry name" value="WAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45656">
        <property type="entry name" value="PROTEIN CBR-CLEC-78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45656:SF4">
        <property type="entry name" value="PROTEIN CBR-CLEC-78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00095">
        <property type="entry name" value="WAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51390">
        <property type="entry name" value="WAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 6" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0768">Sushi</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026034210" description="Sushi domain-containing protein" evidence="6">
        <location>
            <begin position="25"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="WAP" evidence="8">
        <location>
            <begin position="60"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="7">
        <location>
            <begin position="107"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="7">
        <location>
            <begin position="173"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="7">
        <location>
            <begin position="238"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="34"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="142"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007761-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="301" mass="32956" checksum="F5F361108E33E881" modified="2020-08-12" version="1">MLLLKKNLLYWLLLSAILVKTVYCVPTAAVTEADDENDSEWDEDEESSEADDDGRVYKNPRNSPSTECPRDEEQATLLGQKCLRKCSSDEDCKSKKKKCLCDGACGMSCIKPDRECPDLSQPSLGSVTLSGKHFGSRATYTCPHGYHVVGLQSRLCQADGSWAGSEPVCKQNIYCLEPPTIEHARHSALPEQATFDLDSTVQYHCHTGYATAGFPRAKCLAVDGQASWYGPDISCEPRSCGQPPDPAHGWHAGESYTFGGKVTYHCGEGYELVGRAERYCQADGSWTPKELPTCVPQPAAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VUQ4</accession>
    <name>A0A6E8VUQ4_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Palmitoyltransferase</fullName>
            <ecNumber evidence="10">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="10">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8VUQ4"/>
    <dbReference type="EnsemblMetazoa" id="ACON008264-RA">
        <property type="protein sequence ID" value="ACON008264-PA"/>
        <property type="gene ID" value="ACON008264"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002923"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008264"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040216"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883:SF475">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883">
        <property type="entry name" value="ZINC FINGER DHHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0564">Palmitate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="86"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="161"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="271"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="331"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="11">
        <location>
            <begin position="227"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008264-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="382" mass="42810" checksum="5D4D6E6466B4CEE6" modified="2023-06-28" version="2">MKYRASPGQGVVAFPPNILHDSCKRGANVSHPANESELSRARNVLTKSGKLTGSRVGNMLLTFQDRLRIPWRGGAKQITLDNVVTLAILIGTLLLTAVHFYLCLVIMLLLPLVLLYLKRLFGTIHPKTKFFTIWFFGSCVYLVALFELAVPLLEILPQENVGFVVLMTLAFLCLLKAKQRAVLNHLSALPADMNGVESGKGSKEQIVLFSDRDDSDDGATDSDSGRLACQLCRKYVPPRTYHCKVCSSCVLRQDHHNVWLNCCIGKSNHRLYLAGCLFTLLALLVFANLALTAVCHPTPIFTLYGIIVMMPDDCTDIFFQYDIALCFTGSVYALIMASFIAIAIVKQICFISFGLSFSEWRRREHVKRRNCLWNWKAFCFGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VUQ7</accession>
    <name>A0A6E8VUQ7_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">DH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON007712-RB">
        <property type="protein sequence ID" value="ACON007712-PB"/>
        <property type="gene ID" value="ACON007712"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006004"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007712"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034604"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035023">
        <property type="term" value="P:regulation of Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15789">
        <property type="entry name" value="PH_ARHGEF2_18_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041020">
        <property type="entry name" value="PH_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051632">
        <property type="entry name" value="Rho_GEF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13944">
        <property type="entry name" value="AGAP007712-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13944:SF21">
        <property type="entry name" value="CYSTS, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17838">
        <property type="entry name" value="PH_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DH" evidence="8">
        <location>
            <begin position="357"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="7">
        <location>
            <begin position="585"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="108"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="195"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="261"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="999"/>
            <end position="1032"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1192"/>
            <end position="1334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="122"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="135"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="195"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="206"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="261"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="280"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="999"/>
            <end position="1018"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1194"/>
            <end position="1229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1241"/>
            <end position="1274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1292"/>
            <end position="1306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1308"/>
            <end position="1321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1322"/>
            <end position="1334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007712-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1334" mass="149381" checksum="D0CC6DD33239B94F" modified="2020-08-12" version="1">MVPIISVTPHSPGAKFNFLEDTLNQLQCLRESVAHMKNTTLQNTALGGIGSTVSSTKLFSSCPSLPDLTIANPINVWPHHHVLYGLNNDRRKSWTAIEDLTECTKSSHKSVSLSSLDSEEQESLRAAERLHNRTSRNSTGGISTHSLNEAELARDFEKVVAKRNLVPVVPRIPLQKSISTPSIAPVRNQNVKEDNLVSEEYPEHHEKRRKRGSLFFRKKKDKSKTKGQSSVCDACGAVINLATYKEHAVECKAKIAKKYFQAQPKPSSNKKSSANSHNSSQHDDQGRDYYDGNVHNDNANYSDDVPLIRDEFLHEAPIGPHDLGAEPILGVAIDEHDSWSPSVPKEVVKALKDKQVKRQEHIYEFIMTEKHHVQTLLVMQKVFVESLQKHFSHLNLERMFPRLVELTELHTGFLRKLRLKQREHHVVDSIADILLDFFSSMSAQKLKSAYGEFCSNHRSALDTFKCYMTGDNVFAEWYKHCQQNPLLKKKGIPECILFVTQRLTKYPLLIDPLLKSSREDKIEQEKLQKAMSLVKEILVDVDARVADKEKEDRQLEIFKRIDAKSCATFKKDKFKKSDIISCNRKLRFEGVATLMQGRSKMQTVLVVVLSDCLFFLLENSHKYSFFTPENKAGVVSLQKLLIREKAGTESRGIYIISSNPAYPEMYELKVQNPKDKNVWIQSIRAAVIDCPSDESEAEDYMSMEQRQKIIDLKQANMREIICKMRQKDFEQAILLEEKIALQLSLLLDNEQNAEQLGPAVESFISNYGSYRDLISDDCDTIEIWKRVLNSIQEISSLASSLYTAATGLPLSRSSSSVGERQSELFISPTLPKRAETFGGFDERRSKQLITAAGTGGGNLTMHSVNSRDAVLSTLSAGYFTNRETYEKREQQTNAHSSSSPLDVEALLMLANPYGQQKLLGSSNASEGITSDLAKDQNYAALHVSHHLHTLLCIISQQMTTIQSLQHQLNNYRENPKTLYRHNDQLEELRNLQDKLQEEKTAWQKQKEQEERELEETKKSQKAQQEQIRAEQEDIRQQREQLYRKMEILSSQGLLLSPSVALPIPTGVVTSLDEALSVSEEHHVDSAFAGSIPASSMIGSSVTIDRKKDKWRTASITKTPPANLVSATNAAKINATNIKQQLPLKLSSLSSTKAGSTACNMVMSPNSGNVAAAAHGTNSGVTQMFPLKLADKKISSTGTPNHSRTGSSPAVIQQQIQVQSGNPATRTNTYPKIPERFRLRSSDNYPLSSGTTANSPVPQTYQYSPSTHSMGAMSSTPPPLVPARNTAHHESTGQRTSSPISSRHPSLQSPPKSSDTTTSKSDSNNKAKEEEVMYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VV12</accession>
    <name>A0A6E8VV12_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">CD109 antigen</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">TEP1-F</fullName>
        </alternativeName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="24">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Binds covalently through a thioester bond to the pathogen surface resulting in pathogen clearance.</text>
    </comment>
    <comment type="function">
        <text evidence="14">Modulates negatively TGFB1 signaling in keratinocytes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">Heterodimer of a TEP1-N chain and an TEP1-C chain non-covalently linked. Forms a complex composed of TEP1-N and TEP1-C heterodimer, LRIM1 and APL1C; the interaction stabilizes TEP1-N and TEP1-C heterodimer, prevents its binding to tissues while circulating in the hemolymph and protects the thioester bond from hydrolysis. Mature TEP1 and to a lesser extent full-length TEP1 interact with SPCLIP1; the interaction is induced by microbial infection.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Heterodimer; disulfide-linked. Interacts with TGFB1 and TGFBR1. Forms a heteromeric complex with TGFBR1, TGFBR2 and TGFBR3 in a ligand-independent manner.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.</text>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON008364-RE">
        <property type="protein sequence ID" value="ACON008364-PE"/>
        <property type="gene ID" value="ACON008364"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000438"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008364"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037797"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004866">
        <property type="term" value="F:endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02897">
        <property type="entry name" value="A2M_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.20:FF:000001">
        <property type="entry name" value="CD109 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1930:FF:000001">
        <property type="entry name" value="CD109 isoform 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000155">
        <property type="entry name" value="complement C3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.130.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1930">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.690">
        <property type="entry name" value="Alpha-macroglobulin, receptor-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009048">
        <property type="entry name" value="A-macroglobulin_rcpt-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036595">
        <property type="entry name" value="A-macroglobulin_rcpt-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050473">
        <property type="entry name" value="A2M/Complement_sys"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011625">
        <property type="entry name" value="A2M_N_BRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041813">
        <property type="entry name" value="A2M_TED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047565">
        <property type="entry name" value="Alpha-macroglob_thiol-ester_cl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011626">
        <property type="entry name" value="Alpha-macroglobulin_TED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
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    <dbReference type="InterPro" id="IPR008930">
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    </dbReference>
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        <property type="entry name" value="CD109 ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Thiol-ester_cl"/>
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        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00477">
        <property type="entry name" value="ALPHA_2_MACROGLOBULIN"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8" id="KW-0391">Immunity</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0882">Thioester bond</keyword>
    <feature type="domain" description="Alpha-2-macroglobulin bait region" evidence="21">
        <location>
            <begin position="457"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-2-macroglobulin" evidence="22">
        <location>
            <begin position="702"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-macroglobulin receptor-binding" evidence="23">
        <location>
            <begin position="1319"/>
            <end position="1408"/>
        </location>
    </feature>
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        <location>
            <begin position="1431"/>
            <end position="1452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="1438"/>
            <end position="1452"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
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            <dbReference type="ARBA" id="ARBA00022859"/>
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            <dbReference type="ARBA" id="ARBA00023136"/>
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            <dbReference type="ARBA" id="ARBA00023157"/>
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            <dbReference type="ARBA" id="ARBA00023180"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023288"/>
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            <dbReference type="ARBA" id="ARBA00056820"/>
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            <dbReference type="ARBA" id="ARBA00057615"/>
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            <dbReference type="ARBA" id="ARBA00063008"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00063781"/>
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    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00069665"/>
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    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00078071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="SMART" id="SM01359"/>
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    </evidence>
    <evidence type="ECO:0000259" key="22">
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            <dbReference type="SMART" id="SM01360"/>
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    </evidence>
    <evidence type="ECO:0000259" key="23">
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            <dbReference type="SMART" id="SM01361"/>
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    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008364-PE"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
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    </evidence>
    <sequence length="1476" mass="163735" checksum="A8F70515CDE4D1EF" modified="2020-08-12" version="1">MRRSTMFASKKDPAIRILSTSSSSLLAVCLVLSVVLVPAVQCEGHYSIVGAKLLRPNSEYHVAVTNQDVSEPIRFSLAITDASSVIAKQEITLNTGETRLVPFAIGDIPESSYKLVAEGLSGLTFKNETDLEYQQKSFSVFVQTDKSIYKPGDTVRFRVLVLDPNTKPLQKADNISVHINDAKANRIKQWKEGKLVKGVFESELTLSTAPVLGAWTINVEVLGSKHNKVFEVDEYVLPKFEVTVESPGITTFKDGKVKAIIRSKYTYGKPVKGEATVSVSPEFQFNYVQPFAKDVITRKVIPIDGKGSVEFDLREDIHLEGDYSRNIVIEAVVEEELTGRKQNASAKVMIYDRRYKMELVKSDDNFKPGLPYTAWLKVSYQDGAPVQDQTNPVEVKQSSFESTTSVQNYTLDQNGMAKLEINTEVNSSYINVVGVYLGQEFYLHGISKAESDVDSYIRAQVLTEMPLVGKDVLVEVTSTSPMKYFTYQLLGRGDVLLSNTIAVPESKTQSFKFPATFAMVPRAKLVVYYIASNGDMVSDSKVITFDSELQNFMKVSLSKEQSKPGQDVEISISTNPDSYVGLLGVDQSVLLLKSGNDITKQQVFSELEKYEERSYGFYRRKKRFAWNPHAEHRDFSTVGAFVMSNANDPPQKRPVFFSLAARPVPAGVIITSARPFLAATALSASSPVASDPIVVRRTFPESWIWESDEGFSGEKTLQKKVPDTITSWIITGFSVNPIYGLGLTQQPRKLNVFLPFFVSTNLPYSVKRGEVVAIPIVVFNYMEDDQTAEVVLHNDEQEFEFADVENEVVESNKVELFRQKRLDIASNTGKSVSFMVKPKKLGHITIKVTAKTKIAGDAVERQLLVEPEGLPQFINKAAFIDLRAAPELTKTFEVEIPKNAVPDSTRIEVAVIGDVMGSTIQNLDSLIRMPYGCGEQNMLNFVPNIVVLDYLKATNKLTANIEAKAKKFMEAGYQRELGYKHRDGSFSAFGENDKSGSTWLTAFVARSFKQAANHITIDEGVIDKSLEWLSDHQAPNGSFPEVGVVSHKDMQGGSGSGVALTAYTLIAFLENINLVDKYKNTINKAIDYVYRNTESLDDTYALALAAYALQLADHSSKGLILSKLDTKATTDSDSKWWHKPIPETEQKNPWYSRPNSVNVEMSAYGMLAFLEAGLDTDALPIMKWLIGQRNDKGGFQSTQDTVVGLQALAKLAAKITSPNNDVTLTAKINENQEKRMTVNAENGMILQKFELPSAARNIEIQATGSGFAVVQLSYKYNMNVTGEWPRFVLDPQVNANTNPDYLHLSVCASFVPSAGQNVSNMAVMEVGFPSGFTADSDTLPSLENMPFIKKVETKDGDTTVVLYFDSLDQRELCPTISAFRTHKVAKQKPAPVVIYDYYDNSRIARQFYDGPKASLCDICENEDCGEACSIRSQKQRSSDSPSRQPTVEGTMQSGSQTVSVSFFTFLLATLLVRMFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VV48</accession>
    <name>A0A6E8VV48_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Paired amphipathic helix protein Sin3a</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Histone deacetylase complex subunit Sin3a</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Transcriptional corepressor Sin3a</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Acts as a transcriptional repressor. Corepressor for REST. Interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Also interacts with MXD1-MAX heterodimers to repress transcription by tethering SIN3A to DNA. Acts cooperatively with OGT to repress transcription in parallel with histone deacetylation. Involved in the control of the circadian rhythms. Required for the transcriptional repression of circadian target genes, such as PER1, mediated by the large PER complex through histone deacetylation. Cooperates with FOXK1 to regulate cell cycle progression probably by repressing cell cycle inhibitor genes expression. Required for cortical neuron differentiation and callosal axon elongation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Interacts with ARID4B, BRMS1L, HCFC1, HDAC1, HDAC2, MXI1, SAP30L, SAP130, SFPQ and TOPORS. Interacts with OGT (via TPRs 1-6); the interaction mediates transcriptional repression in parallel with histone deacetylase. Interacts with BAZ2A, MXD1, MXD3, MXD4, MBD2, DACH1, NCOR1, NR4A2, REST, RLIM, SAP30, SETDB1, SMYD2, and SUDS3. Interacts with PHF12 in a complex composed of HDAC1, PHF12 and SAP30. Interacts with TET1; the interaction recruits SIN3A to gene promoters. The large PER complex involved in the histone deacetylation is composed of at least HDAC1, PER2, SFPQ and SIN3A. Interacts with KLF11. Interacts with PPHLN1. Found in a complex with YY1, GON4L and HDAC1. Interacts (via PAH2) with FOXK1. Interacts with FOXK2. Found in a complex composed of at least SINHCAF, SIN3A, HDAC1, SAP30, RBBP4, OGT and TET1. Interacts with SINHCAF. Interacts with SPHK2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON007892-RA">
        <property type="protein sequence ID" value="ACON007892-PA"/>
        <property type="gene ID" value="ACON007892"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007122"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007892"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_036386"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070822">
        <property type="term" value="C:Sin3-type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061629">
        <property type="term" value="F:RNA polymerase II-specific DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048511">
        <property type="term" value="P:rhythmic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1160.11:FF:000001">
        <property type="entry name" value="Paired amphipathic helix protein Sin3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1160.11:FF:000002">
        <property type="entry name" value="Paired amphipathic helix protein SIN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1160.11:FF:000004">
        <property type="entry name" value="Paired amphipathic helix protein Sin3a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1160.11">
        <property type="entry name" value="Paired amphipathic helix"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013194">
        <property type="entry name" value="HDAC_interact_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003822">
        <property type="entry name" value="PAH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036600">
        <property type="entry name" value="PAH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039774">
        <property type="entry name" value="Sin3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031693">
        <property type="entry name" value="Sin3_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12346:SF0">
        <property type="entry name" value="SIN3A, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12346">
        <property type="entry name" value="SIN3B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02671">
        <property type="entry name" value="PAH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08295">
        <property type="entry name" value="Sin3_corepress"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16879">
        <property type="entry name" value="Sin3a_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00761">
        <property type="entry name" value="HDAC_interact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47762">
        <property type="entry name" value="PAH2 domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51477">
        <property type="entry name" value="PAH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="10" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="12 18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Histone deacetylase interacting" evidence="20">
        <location>
            <begin position="1071"/>
            <end position="1171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="170"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="372"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="674"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="915"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1349"/>
            <end position="1535"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1760"/>
            <end position="1783"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1806"/>
            <end position="1887"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="372"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="392"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="680"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="19">
        <location>
            <begin position="916"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="1353"/>
            <end position="1368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="1376"/>
            <end position="1429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="1441"/>
            <end position="1455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="1456"/>
            <end position="1471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="1479"/>
            <end position="1499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="1516"/>
            <end position="1527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="19">
        <location>
            <begin position="1766"/>
            <end position="1783"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="19">
        <location>
            <begin position="1808"/>
            <end position="1823"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="1824"/>
            <end position="1871"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00056268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00061761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00068512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00075105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00081271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="SMART" id="SM00761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007892-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1887" mass="197357" checksum="C396FBFECB99FC87" modified="2020-08-12" version="1">MKRGRTEEIQFGQPRVQPPTGQQRIITTTQPHNVASSTGTTIQYQIPPNVIKTATPPDSVNSVTNLSTQQQQQQQGTQLVAPQQATSVQYTTTYNTTLGSNLVKTQQTTNPQQQQIHVVNASNQVRGPTHKSTVVNATAVATNAGTANSNSSVVSSGGVTAGGGQQVITTSMSPGVLPGGGGGAAQGPPPPPPQGQAQLQRLKVEDALSYLDQVKFRFGNQPQVYNDFLDIMKEFKSQSIDTPGVIQRVSNLFRGHPELIVGFNTFLPPGYKIEVQANDQGCYLFQVSVSVPPTASSGASVAPQPSPHKYNTIFQGGGQIATGGPGSGAGGTTVVTASSVGNASGTTGAVNLMAYGGGHAATAAPGASVPIHGGTPLQQPTGGTAGGNASPATAQNAQTLTATTTTTGGASGPQVAQNFSSRSDHHRERTISTGSVASNASLPTGVTASTASSTTVVSAVDATTGGGSAGATVVTGQATPSNIHRILTQQIISQQQQQQQGTGAAGAGMVPAGMVTAPTQVQHHQQTTMQQVQPPPEAQTATVTTTVVPTPTATANQPVEFNHAITYVNKIKNRFHTQPEKYKRFLEILHTYQKEQKTYKEGAQSGCMTSAKQLTEAEVYTQVAQLFDNQEDLLREFGQFLPDATSHHNQAAMQQHHLASGKNNSHNLVVNASMHDHQHQQQQQQQQQQQQQQQQQQQPQQQQLGVPAGTAGIVGLGSGASGPAVHGGGGGGKKLGNSNMAGVGSGAGIGNLKVYNSMQPANMGRLQQERDFSTMGTDGISIHGSGVGGGMPPGSTISVSSGAIRTAGGNIVGTAAVMLEKDRNHMGGVGSGPGSGGVGGVGGGGMNAKYIHGASIATMNATGAMVGGSVLPPGAGPNVVPSVMANLVSANVGGGGSGGGINAIKRSPSYAAGQIGLVGPGGPAGHGNNMSSSRDRGDGSGPPPIKRHKPICRDVSLAEASKYGTLNDYAFFDKVRKALRSPDVYEDFLRCLTLYNQEIVSKMELQTLVSPFLNRFPDLLKWFQDFLGPSTGVGGGAANDCIPLTAAAAAAARQDRDRTQSELAADVDLSTCKRLGASYCALPKSHEGVKCSGRTNLCRDVLNDTWVSFPTWAEDSTFVTSRKTQYEEFIYRCEDERFELDVVIETNSATIRVLEGVQKKLTRMSQDEVSRFRLDDCLGGTSTTIHQRALRRIYGDKAADIIQGLKKNPSVAVPVVLRRMKAKEEEWREAQKSFNKQWREQNEKYYLKSLDHQGINFKQTDIKALRSKSLFNEIETLFDERHEQNDDAAAVPQASGPHMTIPYKDKTILEDAANLLIHHVKRQTGIQKQEKARIKHILRQFVPDLFFAPRQQLSEDEREEGKNDKDTEMEQDEEVSSAGKSSSSGKKSSSNVPSFSGSNSTSNCNPSSNASGAATSTDTSATSASVSEGTGKDGEGGNGGASKTVSMSSTSVDLQSATSSSATVTSSGAEASRTKEDGNGSSNTASGNDDSSTDKSTTIKIEIKEESDASMMQDPASASSGTTGQQPMSPPLPPHAVSKHIEEAYTLFFTNNSWYLFLRLHAILCERLRSIYERAQIIAAEERAYESTRNNSTATALRLKPKSEIRIEEYYNIFLDMLKNLLDGNMESSSYEDTLREMFGIHAYIAFTLDRVVQNAVRQLQHCVTERGALECVELFQAEQRRGGAGGLCRTANRRIAAELAYQRKAEAALQDENCFKIYIYKIDCRVTIELLDTESDDTANNFASTQAYGSYVNRLSNPAATGTGSDSGGGGGGGGGGSGGGGGGVGSGGGSVAAANAAASGSSVAGGASGAGGVGSSGGGSGVAETGASVNSSSGSSSGGNNNHNSTPAGTTNSSSSSNNSAAGGSTGNAEVKTETPDVELVKQNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VWS2</accession>
    <name>A0A6E8VWS2_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Sodium/hydrogen exchanger</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Recycling endosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VWS2"/>
    <dbReference type="EnsemblMetazoa" id="ACON008718-RA">
        <property type="protein sequence ID" value="ACON008718-PA"/>
        <property type="gene ID" value="ACON008718"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_003039"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008718"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031624"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055038">
        <property type="term" value="C:recycling endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015386">
        <property type="term" value="F:potassium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015385">
        <property type="term" value="F:sodium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051453">
        <property type="term" value="P:regulation of intracellular pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098719">
        <property type="term" value="P:sodium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018422">
        <property type="entry name" value="Cation/H_exchanger_CPA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004709">
        <property type="entry name" value="NaH_exchanger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002090">
        <property type="entry name" value="NHE-6/7/9"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00840">
        <property type="entry name" value="b_cpa1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10110">
        <property type="entry name" value="SODIUM/HYDROGEN EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10110:SF187">
        <property type="entry name" value="SODIUM_HYDROGEN EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01084">
        <property type="entry name" value="NAHEXCHNGR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01088">
        <property type="entry name" value="NAHEXCHNGR6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0050">Antiport</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0967">Endosome</keyword>
    <keyword evidence="10 13" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0915">Sodium</keyword>
    <keyword evidence="12 13" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="6 13 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 13" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="62"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="93"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="185"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="214"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="252"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="327"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="382"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="419"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="443"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="483"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="509"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="16">
        <location>
            <begin position="75"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="553"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="623"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="637"/>
            <end position="646"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008718-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="669" mass="74436" checksum="E1F311B4D294E89C" modified="2020-08-12" version="1">MFRRRAAKSATSHQQRSRYRFLPAVVLLVLAQWCLLDAVVTAESTDIELDAKANKIHQIDSLNLLLYTFLLTLTVLTIWLFKHRRVSWLHETGLAVIYGLIVGAIIRYAGTTTPIIHVSVEAEPDAKFNQSLPPDTLWLKFPGNLPHGSDQPVKANKTYTYSFRGELGNVEENEIDLKATFDPEVFFNIILPPIIFHAGYSLKRKYFFRNLGAILMFAIIGTTLSAFLIGALMYGFVQLMPKLKSSFTFLDTLYFGALISPTDPLTILAIFSDMHVDVNLYALVFGESVLNDAVAIVLSGAIQNYGEHYSSNGEFEGHAFLRSLGDFFSVFAFSLLIGASMGCVTAMMTKFTRIRDFPLLESALFVLMSYSTFLIAEAAELTGVVAVLFCGICQAHYTYNNLSDDSRTRTKQIFELLNFLAENFIFSYIGVSMFTFPKHHFDPLFIFTGFMCAAIGRAVNIYPLSALLNIARKPKISWNFQHMLFFAGLRGAMSFALAIRNTVSDARQAMLTTTSLIVITTVIIQGGAANFLLNWLNIPVGVDDETEVLPYQGVRSDLENPDSEGATTPGGTRRGHEKAVLARLWGNFDSRYMKPLLTHSRPTLLETLPVCLSPLARLLTTTEQLTQDGPTRRSDSDSDLCIDDDDRASRGGPADGTGRRNSINRKRAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VWS6</accession>
    <name>A0A6E8VWS6_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="5">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 18">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Perinuclear region</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8VWS6"/>
    <dbReference type="EnsemblMetazoa" id="ACON008412-RB">
        <property type="protein sequence ID" value="ACON008412-PB"/>
        <property type="gene ID" value="ACON008412"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004485"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008412"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037203"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02670">
        <property type="entry name" value="Peptidase_C19N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000009">
        <property type="entry name" value="Putative ubiquitin carboxyl-terminal hydrolase CYLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.190">
        <property type="entry name" value="CAP Gly-rich-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036859">
        <property type="entry name" value="CAP-Gly_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000938">
        <property type="entry name" value="CAP-Gly_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11830">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01302">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01052">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74924">
        <property type="entry name" value="Cap-Gly domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="USP" evidence="15">
        <location>
            <begin position="213"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008412-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="576" mass="64135" checksum="AF2DE244E5105D74" modified="2020-08-12" version="1">MRPTAELNDGAAITANGSIGPADGTSHPTANGLVPRIGSISPESDSGGPAASPLQELPNDPSLGVGSMVEVTLDGGAFEAPGAATAPPATMTDPLLYGVIRWIGPLPTGGGNHRKVMVGVELEDEPIDPTLETTNGTHNGVRLFRCPANRAIFVHTSQCSRDRRFQDIPPMSPGSKTDTNMFGKVDCPVVKGRVPPLKILKLEELEEICGKFKGIQGHHNSCYLDATLFAMFTFTSVFDSLLFRPKEPEDNPQYEEVQRVLLEEIVNPLRKNHFVRADRVLKLRQLLDRLSSVTGLMSEEKDPEEFLNSLLAQILRADPFLKLSSGLDTFYYQLFVEKDERLNLPSVQQLFEQSFLASNIKLKEVPSCLIIQMPRFGKNFKMYPRILPSQVLDVTDIIEDSPRQCWVCGKLAEYECRECFGKMQCEGLEGTAICKSCIDSVHHHSKRLNHKPVPLSVPQDFIPMAPHCEVPRLYMELFAVVCIETSHYVAFVKAASGQDAPWCFFDSMADRNGEQNGYNIPKMVPVPDLPRWLTEEGSRALNEEAVNDKMLPEHAKRLLCDAYMCMYQSTDVMMYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VWS8</accession>
    <name>A0A6E8VWS8_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">CD109 antigen</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">TEP1-F</fullName>
        </alternativeName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="24">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Binds covalently through a thioester bond to the pathogen surface resulting in pathogen clearance.</text>
    </comment>
    <comment type="function">
        <text evidence="14">Modulates negatively TGFB1 signaling in keratinocytes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">Heterodimer of a TEP1-N chain and an TEP1-C chain non-covalently linked. Forms a complex composed of TEP1-N and TEP1-C heterodimer, LRIM1 and APL1C; the interaction stabilizes TEP1-N and TEP1-C heterodimer, prevents its binding to tissues while circulating in the hemolymph and protects the thioester bond from hydrolysis. Mature TEP1 and to a lesser extent full-length TEP1 interact with SPCLIP1; the interaction is induced by microbial infection.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Heterodimer; disulfide-linked. Interacts with TGFB1 and TGFBR1. Forms a heteromeric complex with TGFBR1, TGFBR2 and TGFBR3 in a ligand-independent manner.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.</text>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON008364-RC">
        <property type="protein sequence ID" value="ACON008364-PC"/>
        <property type="gene ID" value="ACON008364"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000438"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008364"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038887"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004866">
        <property type="term" value="F:endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02897">
        <property type="entry name" value="A2M_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.20:FF:000001">
        <property type="entry name" value="CD109 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1930:FF:000001">
        <property type="entry name" value="CD109 isoform 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000155">
        <property type="entry name" value="complement C3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.130.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1930">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.690">
        <property type="entry name" value="Alpha-macroglobulin, receptor-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009048">
        <property type="entry name" value="A-macroglobulin_rcpt-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036595">
        <property type="entry name" value="A-macroglobulin_rcpt-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050473">
        <property type="entry name" value="A2M/Complement_sys"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011625">
        <property type="entry name" value="A2M_N_BRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041813">
        <property type="entry name" value="A2M_TED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047565">
        <property type="entry name" value="Alpha-macroglob_thiol-ester_cl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011626">
        <property type="entry name" value="Alpha-macroglobulin_TED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001599">
        <property type="entry name" value="Macroglobln_a2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019742">
        <property type="entry name" value="MacrogloblnA2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002890">
        <property type="entry name" value="MG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041555">
        <property type="entry name" value="MG3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040839">
        <property type="entry name" value="MG4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11412:SF136">
        <property type="entry name" value="CD109 ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11412">
        <property type="entry name" value="MACROGLOBULIN / COMPLEMENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00207">
        <property type="entry name" value="A2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07703">
        <property type="entry name" value="A2M_BRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07677">
        <property type="entry name" value="A2M_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01835">
        <property type="entry name" value="MG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17791">
        <property type="entry name" value="MG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17789">
        <property type="entry name" value="MG4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07678">
        <property type="entry name" value="TED_complement"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01360">
        <property type="entry name" value="A2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01359">
        <property type="entry name" value="A2M_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01361">
        <property type="entry name" value="A2M_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01419">
        <property type="entry name" value="Thiol-ester_cl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49410">
        <property type="entry name" value="Alpha-macroglobulin receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00477">
        <property type="entry name" value="ALPHA_2_MACROGLOBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8" id="KW-0391">Immunity</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0882">Thioester bond</keyword>
    <feature type="domain" description="Alpha-2-macroglobulin bait region" evidence="21">
        <location>
            <begin position="457"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-2-macroglobulin" evidence="22">
        <location>
            <begin position="693"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-macroglobulin receptor-binding" evidence="23">
        <location>
            <begin position="1310"/>
            <end position="1399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1421"/>
            <end position="1443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="1429"/>
            <end position="1443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00056820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00057615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00063008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00063781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00069665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00078071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="SMART" id="SM01359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="SMART" id="SM01360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="SMART" id="SM01361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008364-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1467" mass="163006" checksum="720F651BBEE9933D" modified="2020-08-12" version="1">MRRSTMFASKKDPAIRILSTSSSSLLAVCLVLSVVLVPAVQCEGHYSIVGAKLLRPNSEYHVAVTNQDVSEPIRFSLAITDASSVIAKQEITLNTGETRLVPFAIGDIPESSYKLVAEGLSGLTFKNETDLEYQQKSFSVFVQTDKSIYKPGDTVRFRVLVLDPNTKPLQKADNISVHINDAKANRIKQWKEGKLVKGVFESELTLSTAPVLGAWTINVEVLGSKHNKVFEVDEYVLPKFEVTVESPGITTFKDGKVKAIIRSKYTYGKPVKGEATVSVSPEFQFNYVQPFAKDVITRKVIPIDGKGSVEFDLREDIHLEGDYSRNIVIEAVVEEELTGRKQNASAKVMIYDRRYKMELVKSDDNFKPGLPYTAWLKVSYQDGAPVQDQTNPVEVKQSSFESTTSVQNYTLDQNGMAKLEINTEVNSSYINVVGVYLGQEFYLHGISKAESDVDSYIRAQVLTEMPLVGKDVLVEVTSTSPMKYFTYQLLGRGDVLLSNTIAVPESKTQSFKFPATFAMVPRAKLVVYYIASNGDMVSDSKVITFDSELQNFMKVSLSKEQSKPGQDVEISISTNPDSYVGLLGVDQSVLLLKSGNDITKQQVFSELEKYEERSYGFYRRKKRFAWNPHAEHRDFSTVGAFVMSNANDPPLPPIVLMPMAAMGYTVTSAVVKRPFSRPRVLAPAPVRKEFPETWLWQTISSFSGEKTLQKKVPDTITSWIITGFSVNPIYGLGLTQQPRKLNVFLPFFVSTNLPYSVKRGEVVAIPIVVFNYMEDDQTAEVVLHNDEQEFEFADVENEVVESNKVELFRQKRLDIASNTGKSVSFMVKPKKLGHITIKVTAKTKIAGDAVERQLLVEPEGLPQFINKAAFIDLRAAPELTKTFEVEIPKNAVPDSTRIEVAVIGDVMGSTIQNLDSLIRMPYGCGEQNMLNFVPNIVVLDYLKATNKLTANIEAKAKKFMEAGYQRELGYKHRDGSFSAFGENDKSGSTWLTAFVARSFKQAANHITIDEGVIDKSLEWLSDHQAPNGSFPEVGVVSHKDMQGGSGSGVALTAYTLIAFLENINLVDKYKNTINKAIDYVYRNTESLDDTYALALAAYALQLADHSSKGLILSKLDTKATTDSDSKWWHKPIPETEQKNPWYSRPNSVNVEMSAYGMLAFLEAGLDTDALPIMKWLIGQRNDKGGFQSTQDTVVGLQALAKLAAKITSPNNDVTLTAKINENQEKRMTVNAENGMILQKFELPSAARNIEIQATGSGFAVVQLSYKYNMNVTGEWPRFVLDPQVNANTNPDYLHLSVCASFVPSAGQNVSNMAVMEVGFPSGFTADSDTLPSLENMPFIKKVETKDGDTTVVLYFDSLDQRELCPTISAFRTHKVAKQKPAPVVIYDYYDNSRIARQFYDGPKASLCDICENEDCGEACSIRSQKQRSSDSPSRQPTVEGTMQSGSQTVSVSFFTFLLATLLVRMFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VWT3</accession>
    <name>A0A6E8VWT3_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">CD109 antigen</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">TEP1-F</fullName>
        </alternativeName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="24">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Binds covalently through a thioester bond to the pathogen surface resulting in pathogen clearance.</text>
    </comment>
    <comment type="function">
        <text evidence="14">Modulates negatively TGFB1 signaling in keratinocytes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">Heterodimer of a TEP1-N chain and an TEP1-C chain non-covalently linked. Forms a complex composed of TEP1-N and TEP1-C heterodimer, LRIM1 and APL1C; the interaction stabilizes TEP1-N and TEP1-C heterodimer, prevents its binding to tissues while circulating in the hemolymph and protects the thioester bond from hydrolysis. Mature TEP1 and to a lesser extent full-length TEP1 interact with SPCLIP1; the interaction is induced by microbial infection.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Heterodimer; disulfide-linked. Interacts with TGFB1 and TGFBR1. Forms a heteromeric complex with TGFBR1, TGFBR2 and TGFBR3 in a ligand-independent manner.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.</text>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON008364-RH">
        <property type="protein sequence ID" value="ACON008364-PH"/>
        <property type="gene ID" value="ACON008364"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000438"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008364"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038887"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004866">
        <property type="term" value="F:endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02897">
        <property type="entry name" value="A2M_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.20:FF:000001">
        <property type="entry name" value="CD109 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1930:FF:000001">
        <property type="entry name" value="CD109 isoform 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000155">
        <property type="entry name" value="complement C3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.130.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1930">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.690">
        <property type="entry name" value="Alpha-macroglobulin, receptor-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009048">
        <property type="entry name" value="A-macroglobulin_rcpt-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036595">
        <property type="entry name" value="A-macroglobulin_rcpt-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050473">
        <property type="entry name" value="A2M/Complement_sys"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011625">
        <property type="entry name" value="A2M_N_BRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041813">
        <property type="entry name" value="A2M_TED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047565">
        <property type="entry name" value="Alpha-macroglob_thiol-ester_cl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011626">
        <property type="entry name" value="Alpha-macroglobulin_TED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001599">
        <property type="entry name" value="Macroglobln_a2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019742">
        <property type="entry name" value="MacrogloblnA2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002890">
        <property type="entry name" value="MG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041555">
        <property type="entry name" value="MG3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040839">
        <property type="entry name" value="MG4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11412:SF136">
        <property type="entry name" value="CD109 ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11412">
        <property type="entry name" value="MACROGLOBULIN / COMPLEMENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00207">
        <property type="entry name" value="A2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07703">
        <property type="entry name" value="A2M_BRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07677">
        <property type="entry name" value="A2M_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01835">
        <property type="entry name" value="MG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17791">
        <property type="entry name" value="MG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17789">
        <property type="entry name" value="MG4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07678">
        <property type="entry name" value="TED_complement"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01360">
        <property type="entry name" value="A2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01359">
        <property type="entry name" value="A2M_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01361">
        <property type="entry name" value="A2M_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01419">
        <property type="entry name" value="Thiol-ester_cl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49410">
        <property type="entry name" value="Alpha-macroglobulin receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00477">
        <property type="entry name" value="ALPHA_2_MACROGLOBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8" id="KW-0391">Immunity</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0882">Thioester bond</keyword>
    <feature type="domain" description="Alpha-2-macroglobulin bait region" evidence="21">
        <location>
            <begin position="457"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-2-macroglobulin" evidence="22">
        <location>
            <begin position="677"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-macroglobulin receptor-binding" evidence="23">
        <location>
            <begin position="1308"/>
            <end position="1397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1419"/>
            <end position="1441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="1427"/>
            <end position="1441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00056820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00057615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00063008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00063781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00069665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00078071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="SMART" id="SM01359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="SMART" id="SM01360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="SMART" id="SM01361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008364-PH"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1465" mass="162623" checksum="0BE4A5BB9EF9B1B1" modified="2020-08-12" version="1">MRRSTMFASKKDPAIRILSTSSSSLLAVCLVLSVVLVPAVQCEGHYSIVGAKLLRPNSEYHVAVTNQDVSEPIRFSLAITDASSVIAKQEITLNTGETRLVPFAIGDIPESSYKLVAEGLSGLTFKNETDLEYQQKSFSVFVQTDKSIYKPGDTVRFRVLVLDPNTKPLQKADNISVHINDAKANRIKQWKEGKLVKGVFESELTLSTAPVLGAWTINVEVLGSKHNKVFEVDEYVLPKFEVTVESPGITTFKDGKVKAIIRSKYTYGKPVKGEATVSVSPEFQFNYVQPFAKDVITRKVIPIDGKGSVEFDLREDIHLEGDYSRNIVIEAVVEEELTGRKQNASAKVMIYDRRYKMELVKSDDNFKPGLPYTAWLKVSYQDGAPVQDQTNPVEVKQSSFESTTSVQNYTLDQNGMAKLEINTEVNSSYINVVGVYLGQEFYLHGISKAESDVDSYIRAQVLTEMPLVGKDVLVEVTSTSPMKYFTYQLLGRGDVLLSNTIAVPESKTQSFKFPATFAMVPRAKLVVYYIASNGDMVSDSKVITFDSELQNFMKVSLSKEQSKPGQDVEISISTNPDSYVGLLGVDQSVLLLKSGNDITKQQVFSELEKYEERSYGFYRRKKRFAWNPHAEHRDFSTVGAFVMSNANDPPQYYMPFHRAGMEGIPMVQMAAIPVADVYFAESEVDSGPPASPALVTRKDFSGEKTLQKKVPDTITSWIITGFSVNPIYGLGLTQQPRKLNVFLPFFVSTNLPYSVKRGEVVAIPIVVFNYMEDDQTAEVVLHNDEQEFEFADVENEVVESNKVELFRQKRLDIASNTGKSVSFMVKPKKLGHITIKVTAKTKIAGDAVERQLLVEPEGLPQFINKAAFIDLRAAPELTKTFEVEIPKNAVPDSTRIEVAVIGDVMGSTIQNLDSLIRMPYGCGEQNMLNFVPNIVVLDYLKATNKLTANIEAKAKKFMEAGYQRELGYKHRDGSFSAFGENDKSGSTWLTAFVARSFKQAANHITIDEGVIDKSLEWLSDHQAPNGSFPEVGVVSHKDMQGGSGSGVALTAYTLIAFLENINLVDKYKNTINKAIDYVYRNTESLDDTYALALAAYALQLADHSSKGLILSKLDTKATTDSDSKWWHKPIPETEQKNPWYSRPNSVNVEMSAYGMLAFLEAGLDTDALPIMKWLIGQRNDKGGFQSTQDTVVGLQALAKLAAKITSPNNDVTLTAKINENQEKRMTVNAENGMILQKFELPSAARNIEIQATGSGFAVVQLSYKYNMNVTGEWPRFVLDPQVNANTNPDYLHLSVCASFVPSAGQNVSNMAVMEVGFPSGFTADSDTLPSLENMPFIKKVETKDGDTTVVLYFDSLDQRELCPTISAFRTHKVAKQKPAPVVIYDYYDNSRIARQFYDGPKASLCDICENEDCGEACSIRSQKQRSSDSPSRQPTVEGTMQSGSQTVSVSFFTFLLATLLVRMFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VWW6</accession>
    <name>A0A6E8VWW6_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="6">MIF4G domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VWW6"/>
    <dbReference type="EnsemblMetazoa" id="ACON008763-RB">
        <property type="protein sequence ID" value="ACON008763-PB"/>
        <property type="gene ID" value="ACON008763"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005310"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008763"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042502"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008494">
        <property type="term" value="F:translation activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006446">
        <property type="term" value="P:regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.180:FF:000039">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003890">
        <property type="entry name" value="MIF4G-like_typ-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051367">
        <property type="entry name" value="mRNA_TranslReg/HistoneTransl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23254:SF16">
        <property type="entry name" value="CBP80_20-DEPENDENT TRANSLATION INITIATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23254">
        <property type="entry name" value="EIF4G DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02854">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00543">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="MIF4G" evidence="5">
        <location>
            <begin position="195"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="36"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008763-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="433" mass="48205" checksum="A078BA2364998A0D" modified="2023-06-28" version="2">MAEQNENAATKKSVKIQDYISPGISRERSFIRTSNQQMNNSNKRRSLAFTQSQAHQHAQQQLREGRLDGLGAMNNKLNVHAQEFQMNNLPLNDHRFPLQSSRSLNIYSSSLQHSKSSVTMPLGSMPGRHHALQLGSIGSPVPRGSLMMPAHAPLHHVGVQLQPSHMPLVNSPSSSNILHRSKSLSSADTLTRGLASLGLGIGSEANDIGMFSPEVQTAITKAIEDPNQLNARCLMDLAAQLLHRAVEGRRYSLPISRLCISIIAKEKKETFLEALLNTCRQWYQERDKVLGAMQNSKNPSRPRFTSFMAFLTEMFCQLKRRQLQLRTECDGVPPPLVLLTVLGKCCEDCVQPPVRSLSEIECLFFVLTCIGRDLETHLPQQLESLLAGVRDAFLNSAASAPAIRRTLLQLIELQASHWQLPGNTVLYYYPSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VX55</accession>
    <name>A0A6E8VX55_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Insulin receptor substrate 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Protein chico</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit. May mediate the control of various cellular processes by insulin-like peptides. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains. Involved in control of cell proliferation, cell size, and body and organ growth throughout development. Also has a role in a signaling pathway controlling the physiological response required to endure periods of low nutrient conditions. Insulin/insulin-like growth factor (IGF) signaling pathway has a role in regulating aging and is necessary in the ovary for vitellogenic maturation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Bindings to phosphatidylinositol 3-kinase and SHP2.</text>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON008859-RB">
        <property type="protein sequence ID" value="ACON008859-PB"/>
        <property type="gene ID" value="ACON008859"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_003426"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008859"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038234"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005158">
        <property type="term" value="F:insulin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043548">
        <property type="term" value="F:phosphatidylinositol 3-kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008286">
        <property type="term" value="P:insulin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048477">
        <property type="term" value="P:oogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01257">
        <property type="entry name" value="PH_IRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01204">
        <property type="entry name" value="PTB_IRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039011">
        <property type="entry name" value="IRS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002404">
        <property type="entry name" value="IRS_PTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10614">
        <property type="entry name" value="INSULIN RECEPTOR SUBSTRATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10614:SF13">
        <property type="entry name" value="INSULIN RECEPTOR SUBSTRATE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02174">
        <property type="entry name" value="IRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00628">
        <property type="entry name" value="INSULINRSI"/>
    </dbReference>
    <dbReference type="SMART" id="SM01244">
        <property type="entry name" value="IRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00310">
        <property type="entry name" value="PTBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51064">
        <property type="entry name" value="IRS_PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0221">Differentiation</keyword>
    <keyword evidence="4" id="KW-0341">Growth regulation</keyword>
    <keyword evidence="7" id="KW-0896">Oogenesis</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PH" evidence="11">
        <location>
            <begin position="208"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="IRS-type PTB" evidence="12">
        <location>
            <begin position="332"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="48"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="143"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="496"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="850"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="903"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="936"/>
            <end position="1141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1156"/>
            <end position="1223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1313"/>
            <end position="1426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1468"/>
            <end position="1487"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1587"/>
            <end position="1723"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1771"/>
            <end position="1810"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1830"/>
            <end position="1867"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="49"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="65"/>
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        <source>
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        </source>
    </evidence>
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        <source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00033282"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00046145"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008859-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1867" mass="194268" checksum="5073BD72F5FF4E93" modified="2020-08-12" version="1">MSTLTNNANNSSSNSNSNNNNNSSSNNTNNNNNSIASYYQQYIQHYQLSPQGGTAPPSAGGSPTVTSINNNNNGPTSDNIHTINNNNISTSSSSTSSNQSQPTTAANSGSGGGGGVVGGILGVREVSAKLFLPFARAKMMHSSSSAASTPAKDQHQPGTPLDGTSMAGMNATPTGTPSATPGTTTGGSSAGMSGAGRASSLIIPKALNVVLHGYHRKHKTNKKKYFVVYGDVPGKAARLEYFDSEKKFKQSFQKGSAGCDGGTQPKRTIVLRTCFNINKRHDLKKQIISLYTKDDSFCIVFESEEELNLWLRTLLRLQRGEDSEGDPPRPTFEHVWEVYVQRKGLGDSYGILGNYRLCITDKTLSLVRIGSELTPHQEKRAENVEFLLASVRRCGHSQRYFYLEVGRSSKTGAGEIWMEVQDSIIAQNMYTTIMKSAKSKDDNLGPMIRIRSSSANAASKPTSMLFRRQTHTGQKPINCSPSSGVAIARERCDSLPSRNRTTSETMPHQQQQQQQHLLNQQQQQQHHHQLMLNSGSMPPPRMIPSSNRPHSVHISHSHSPPVNSSPLSPPPPATYSTGSDGSSLSIDESTDSFITGSLTPDEGQGYPKVINQLNSGCSIPEENSEEFIKDYGHIDLSAAGCGISQHDDFAVPAARRGGSPAPPSTVGSVEGSAYMEMCSPSGSSPGDPSGSCYMAMSPVADFSRGLCSSSSAHSRASSLAEDIVDGYVPMSMPRTAAAAPAAPPTEEYMLMDPTLNGGGTSSRLSNATAASNNAGSSIIGGGGMSSAASSCSITSGTPSADLRLSLEKVPAYIGQSDEHEVNDRPFRTYSVGSRPELIKRKLRVEMLSSGESSVGGSSAGGSASSRQRAFSVGSRAKVPRCSDVYKGYSAAPVIVSFASTSSQGSMLESSNNNSSSSTSSISHAGSYGASIGDTLPMDELAGGGSAGGSSMGRKSGSGSLLPDGAGGGKPPSHGGSHGRMSDLMEIDYSSPRQQPARPSTRSSEPSGMEDYLDMSGNNNEEEQRARGQGATSSGYVEMRPGRLNSQSEENDYLNMTPGGGRDEPDCCPAAASIATSSPIQIQHHAKQPADGAGSAHSSSSNNNNNNNYLEMYPRAAVAQKEQKRTSSATSTTTTATKVPSEDYLNMAPLMISSLGEASGSEEEKMHGPDEEPLLESSSAPPDGYMEMSWNHHGPVTATGPVSSSASSSATSSARTVPTNGGDDYMNMSCGSGSQSEDASRTYSAPITIQPGAGKCRLDLSGESKVEAGANIPSYLPLGQHPDARKSVHHPHHQSAMIVALQQHLPLAAQAPIQTTTGQQSGSIGFSPSKPAETTPERARCDSRDSGIVTPSGSQPTIFPFSPASPTKPFAPAQDEPMGGESLDDLSQNYAELSLGRQQQQQLQQAQTEPSPAIRKLSTGSNRGSGSFSKLQLVASQPDYVNYCPATPPISGDVAPPASNMSLNVLPSAQSRTRSSIDDDGAGDYALMNPASSRKLSTTSMGSMGAASLSPAGVVPPGSSAVGSITPPVLVARKSTLLLSSPQMGFKPIASSQDAELLLQTKAVPHATANSPKALNLGRRTGEPAFSFTEQQQQQMAPLQRSISTGTRPNSVNSELIGRPIGTPAGPSTPGAASHSSATAAGSTRPSSANSERLPVISATSSSASSTSTLCESKNQSPSASSTALAMVVGSSTTTSPPSVPGSRPDSVSSITNPHIVSRPPSVSSERELHYASLDLPPCSSSGKQTVAASPFAPGTMMLLAAAAAATTPAATSSAAAPVTKMDVDEPLPGSNEARTSIDSSSPTTAAGLQQQQQPVRCAAFTYAQIDFVRSQAQSQQGQTGTGPAGASGPTGSTTTTTTSTPPQSSQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VX63</accession>
    <name>A0A6E8VX63_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Epidermal growth factor receptor substrate 15-like 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VX63"/>
    <dbReference type="EnsemblMetazoa" id="ACON009189-RA">
        <property type="protein sequence ID" value="ACON009189-PA"/>
        <property type="gene ID" value="ACON009189"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002033"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON009189"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_036043"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030132">
        <property type="term" value="C:clathrin coat of coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045296">
        <property type="term" value="F:cadherin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00052">
        <property type="entry name" value="EH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000261">
        <property type="entry name" value="EH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11216">
        <property type="entry name" value="EH DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11216:SF176">
        <property type="entry name" value="EPIDERMAL GROWTH FACTOR RECEPTOR PATHWAY SUBSTRATE CLONE 15, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12763">
        <property type="entry name" value="EH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00027">
        <property type="entry name" value="EH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50031">
        <property type="entry name" value="EH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EH" evidence="3">
        <location>
            <begin position="15"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="EH" evidence="3">
        <location>
            <begin position="126"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="158"/>
            <end position="193"/>
        </location>
    </feature>
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        <location>
            <begin position="299"/>
            <end position="388"/>
        </location>
    </feature>
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            <begin position="302"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="491"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="596"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="676"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="596"/>
            <end position="609"/>
        </location>
    </feature>
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        <location>
            <begin position="723"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="738"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="791"/>
            <end position="800"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="800" mass="86442" checksum="4E7AD6AEA18F2E9C" modified="2020-08-12" version="1">MPDIVKIAGDHILIYEAYYKQLDPKEANEIGALDAAKFLKKSGLSDVVLSRIWDLSDPNGKGFLTKEGFFVSLKLIGLAQEGSEINLKNIYNVLSKPPKVGDLPKVPAQVKLLPVESTDWSMKPEKRQQYEQLFDSLGPMNGLLPGAKVRMTLMNSKLPVETLGRIWDLADQDRDGSLDKHEFCVAMHLVYEALDKRAIPAMLPPQLQRNYAPHPPQNGGGFDAFGGGAADGAGGFVANFPSDIAPPPVVPPLPAALARPPPMTAPVIPPVPMGLGGVPLVSAPAPPIEVTSWVVSPLERCKYEEIFNKSDTDRDGLVSGLEIKDVFLQSGVAQNKLAHIWALCDTNQSGKLKLEEFCLAMWFVDRAKKGIDPPQALAPNMVPPSLRKSSLIQAQEPPQPTYSNPELEMISKEIDELAKERRLLEQEVAQKEADVRIKGGELRSLQSELDTLTATLKQLENQKGEAQKRLDDLKNQVTKIREQCQKQEATLKEQEGELDSRRSELQKLRDEEQSLEKEYNTSTKEVDRLTSQLQDTQLEISQVKAMVTQIQEYQRQMTDALSMFRSAIESNDPILVSDYSLKIEPEFREAKQALEEKEVENANKRDPFGDNRSNGFGAGEPAETGFGDDFKSSNGFATQFDVGSNGGFHGGTAAAGGFGENGFGAFGAKAPSHAIGGGAADPFGSSAAADPFGDRKGSGSVAAEPAKDEFGCDPFAILHAPTTAGQALSPSPSKSVAPPRPESPSPALPPKKAKQPPPRPAPPRPMQGPTPTKPAPPASDAFGDSSGGGSFANFADFDNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VYV2</accession>
    <name>A0A6E8VYV2_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Gustatory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Gustatory receptor which mediates acceptance or avoidance behavior, depending on its substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the insect chemoreceptor superfamily. Gustatory receptor (GR) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VYV2"/>
    <dbReference type="EnsemblMetazoa" id="ACON009805-RK">
        <property type="protein sequence ID" value="ACON009805-PK"/>
        <property type="gene ID" value="ACON009805"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACON009805"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007635">
        <property type="term" value="P:chemosensory behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008049">
        <property type="term" value="P:male courtship behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050909">
        <property type="term" value="P:sensory perception of taste"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013604">
        <property type="entry name" value="7TM_chemorcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21143:SF134">
        <property type="entry name" value="GUSTATORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21143">
        <property type="entry name" value="INVERTEBRATE GUSTATORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08395">
        <property type="entry name" value="7tm_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="78"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="133"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="168"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="248"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="280"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="360"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009805-PK"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="403" mass="46297" checksum="EEF6835410674002" modified="2020-08-12" version="1">MDVFDNIRGLIRCLRLLGLFSIHQYRHTGNTFECSRNAALKFVIFLTITLACSGYSMYRNILDYPAMAAMNNTSIEPFFYIAHTITVCVVLCVLPVQTVGRRHQLAYLMNILIKNEEDLVKLTSGGRSTNYRLVTLLATVCLWNGLLFHVFFHFYYIDEFRHFGDRFVSMYIVSCFFMYIDLAMELLLGLCGCLLLVAQLQLGRLAQAAREFDYGVDSPERFFFRYGVIHNRTAFAIRDHLSHYFGPMLAVFCTYVSLEVAICLLAIVNSMTNPMGQSKFYIVVVVLWPLTDVKKLFAVILLSERTKQVVEETALCTRHFDDYRLQNTRAAKQIQKFLLKNLHQKKKFSACGFFDIDNTVIYMVFSSIVTYLVILIQFKQLETDLTQAGDGYNVTSNVSTVQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VYV6</accession>
    <name>A0A6E8VYV6_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Fructose-1,6-bisphosphatase isozyme 2</fullName>
            <ecNumber evidence="8">3.1.3.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">D-fructose-1,6-bisphosphate 1-phosphohydrolase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">D-fructose-1,6-bisphosphate 1-phosphohydrolase 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">Muscle FBPase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="27 28">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="27">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="19">Catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate in the presence of divalent cations and probably participates in glycogen synthesis from carbohydrate precursors, such as lactate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>beta-D-fructose 1,6-bisphosphate + H2O = beta-D-fructose 6-phosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:11064"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="EC" id="3.1.3.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="6">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="20">Homotetramer. Interacts with ALDOA; the interaction blocks inhibition by physiological concentrations of AMP and reduces inhibition by Ca(2+). Interacts with alpha-actinin and F-actin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Myofibril</location>
            <location evidence="4">Sarcomere</location>
            <location evidence="4">Z line</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 24">Belongs to the FBPase class 1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.11" evidence="8"/>
    <dbReference type="RefSeq" id="XP_049466428.1">
        <property type="nucleotide sequence ID" value="XM_049610471.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VYV6"/>
    <dbReference type="EnsemblMetazoa" id="ACON009173-RA">
        <property type="protein sequence ID" value="ACON009173-PA"/>
        <property type="gene ID" value="ACON009173"/>
    </dbReference>
    <dbReference type="GeneID" id="125907538"/>
    <dbReference type="CTD" id="35265"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_008834"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON009173"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038929"/>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070161">
        <property type="term" value="C:anchoring junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042132">
        <property type="term" value="F:fructose 1,6-bisphosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030388">
        <property type="term" value="P:fructose 1,6-bisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006000">
        <property type="term" value="P:fructose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005986">
        <property type="term" value="P:sucrose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00354">
        <property type="entry name" value="FBPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.80:FF:000001">
        <property type="entry name" value="Fructose-1,6-bisphosphatase class 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.540.10:FF:000005">
        <property type="entry name" value="Fructose-1,6-bisphosphatase isozyme 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.540.10">
        <property type="entry name" value="Fructose-1,6-Bisphosphatase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01855">
        <property type="entry name" value="FBPase_class1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044015">
        <property type="entry name" value="FBPase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000146">
        <property type="entry name" value="FBPase_class-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033391">
        <property type="entry name" value="FBPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028343">
        <property type="entry name" value="FBPtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020548">
        <property type="entry name" value="Fructose_bisphosphatase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006778">
        <property type="entry name" value="PRK09293.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11556">
        <property type="entry name" value="FRUCTOSE-1,6-BISPHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11556:SF1">
        <property type="entry name" value="FRUCTOSE-BISPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00316">
        <property type="entry name" value="FBPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18913">
        <property type="entry name" value="FBPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500210">
        <property type="entry name" value="FBPtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000904">
        <property type="entry name" value="FBPtase_SBPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00115">
        <property type="entry name" value="F16BPHPHTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56655">
        <property type="entry name" value="Carbohydrate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00124">
        <property type="entry name" value="FBPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0106">Calcium</keyword>
    <keyword evidence="17 24" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="15" id="KW-0965">Cell junction</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12 24" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fructose-1-6-bisphosphatase class I N-terminal" evidence="25">
        <location>
            <begin position="13"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="Fructose-1-6-bisphosphatase class 1 C-terminal" evidence="26">
        <location>
            <begin position="206"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00010941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00013093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00032973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00037516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00038670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00040321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00042757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00043165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="RuleBase" id="RU000508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="Pfam" id="PF00316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="Pfam" id="PF18913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009173-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="336" mass="36738" checksum="F130352E3A952E86" modified="2020-08-12" version="1">MTQQGPAFDSNCMTLTRFVLQEQKKYKHATGDLSQLLNCIQTAIKAISSAVRKAGIAKLQGISGDTNVQGEQVKKLDVLSNEIFINMLKSSYATCLLVSEENDNVIEIETDKRGKYVVSFDPLDGSSNIDCLVSIGSIFAITKQVKENTTPSEQDALQPGNKIVAAGYALYGSATMIVISLGNGVHGFMYDPSIGEFVLTDYNMRIPERGNIYSINEGYASTWDESVLNYVKDKKDPAKGKPYGARYVGSMVADVHRTIKYGGIFIYPATAAAKNGKLRLLYECNPMAYLVTQAGGKAYATKGKEILDVMPTSIHQRSPIYLGSKLDVEEAISYIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VYX4</accession>
    <name>A0A6E8VYX4_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">S-adenosylmethionine synthase</fullName>
            <ecNumber evidence="11">2.5.1.6</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the formation of S-adenosylmethionine from methionine and ATP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>L-methionine + ATP + H2O = S-adenosyl-L-methionine + phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21080"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="2.5.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
        <text evidence="11">Binds 1 potassium ion per subunit. The potassium ion interacts primarily with the substrate.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="11">Binds 2 magnesium ions per subunit. The magnesium ions interact primarily with the substrate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the AdoMet synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.6" evidence="11"/>
    <dbReference type="RefSeq" id="XP_040232176.1">
        <property type="nucleotide sequence ID" value="XM_040376242.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040232177.1">
        <property type="nucleotide sequence ID" value="XM_040376243.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040232178.1">
        <property type="nucleotide sequence ID" value="XM_040376244.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_049466488.1">
        <property type="nucleotide sequence ID" value="XM_049610531.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_049466489.1">
        <property type="nucleotide sequence ID" value="XM_049610532.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VYX4"/>
    <dbReference type="EnsemblMetazoa" id="ACON009447-RA">
        <property type="protein sequence ID" value="ACON009447-PA"/>
        <property type="gene ID" value="ACON009447"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON009447-RB">
        <property type="protein sequence ID" value="ACON009447-PB"/>
        <property type="gene ID" value="ACON009447"/>
    </dbReference>
    <dbReference type="GeneID" id="120955402"/>
    <dbReference type="KEGG" id="acoz:120955402"/>
    <dbReference type="CTD" id="48552"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010780"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON009447"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031941"/>
    <dbReference type="UniPathway" id="UPA00315">
        <property type="reaction ID" value="UER00080"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004478">
        <property type="term" value="F:methionine adenosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006556">
        <property type="term" value="P:S-adenosylmethionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd18079">
        <property type="entry name" value="S-AdoMet_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.10:FF:000001">
        <property type="entry name" value="S-adenosylmethionine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.10:FF:000003">
        <property type="entry name" value="S-adenosylmethionine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.10:FF:000004">
        <property type="entry name" value="S-adenosylmethionine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00086">
        <property type="entry name" value="S_AdoMet_synth1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022631">
        <property type="entry name" value="ADOMET_SYNTHASE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022630">
        <property type="entry name" value="S-AdoMet_synt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022629">
        <property type="entry name" value="S-AdoMet_synt_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022628">
        <property type="entry name" value="S-AdoMet_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002133">
        <property type="entry name" value="S-AdoMet_synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022636">
        <property type="entry name" value="S-AdoMet_synthetase_sfam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01034">
        <property type="entry name" value="metK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11964">
        <property type="entry name" value="S-ADENOSYLMETHIONINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02773">
        <property type="entry name" value="S-AdoMet_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02772">
        <property type="entry name" value="S-AdoMet_synt_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00438">
        <property type="entry name" value="S-AdoMet_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000497">
        <property type="entry name" value="MAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55973">
        <property type="entry name" value="S-adenosylmethionine synthetase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00376">
        <property type="entry name" value="ADOMET_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00377">
        <property type="entry name" value="ADOMET_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 11" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="9 11" id="KW-0630">Potassium</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="S-adenosylmethionine synthetase N-terminal" evidence="13">
        <location>
            <begin position="26"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="S-adenosylmethionine synthetase central" evidence="14">
        <location>
            <begin position="137"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="S-adenosylmethionine synthetase C-terminal" evidence="15">
        <location>
            <begin position="260"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009447-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="404" mass="44518" checksum="7F7428A75366B9E6" modified="2020-08-12" version="1">MPQTNGFAASNSNGHSGYEMEDGCSFLFTSESVGEGHPDKMCDQISDAILDAHLQQDPNAKVACETISKTGMILLCGEITSKAVIDYQRVVRETVKHIGYDDSSKGFDYKTCNVLLALDQQSPNIAAGVHVNRPEEEVGAGDQGLMFGYATDETEECMPLTVVLAHRLNEKIAELRRSGDFWWARPDSKTQVTAEYVFESGACIPQRVHTVVVSLQHSDKITLEELRKEIMDKVIKDVIPAKYFDANTIVHINPCGLFIIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKDFTKVDRSAAYAARWVAKSLVKAGICRRCLVQVAYAIGLAEPLSITVFHYGTSKYNQQELLKIVSDNFDLRPGKIVKDLNLRTPFYQRTSTYGHFGRPGFPWEEPKELVIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VYX9</accession>
    <name>A0A6E8VYX9_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="3">S-adenosylmethionine synthase</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VYX9"/>
    <dbReference type="EnsemblMetazoa" id="ACON009447-RF">
        <property type="protein sequence ID" value="ACON009447-PF"/>
        <property type="gene ID" value="ACON009447"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010780"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON009447"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031941"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004478">
        <property type="term" value="F:methionine adenosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006556">
        <property type="term" value="P:S-adenosylmethionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022628">
        <property type="entry name" value="S-AdoMet_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002133">
        <property type="entry name" value="S-AdoMet_synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022636">
        <property type="entry name" value="S-AdoMet_synthetase_sfam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11964">
        <property type="entry name" value="S-ADENOSYLMETHIONINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00438">
        <property type="entry name" value="S-AdoMet_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55973">
        <property type="entry name" value="S-adenosylmethionine synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S-adenosylmethionine synthetase N-terminal" evidence="2">
        <location>
            <begin position="26"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009447-PF"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="107" mass="11544" checksum="9953FFD7F9C11B78" modified="2020-08-12" version="1">MPQTNGFAASNSNGHSGYEMEDGCSFLFTSESVGEGHPDKMCDQISDAILDAHLQQDPNAKVACETISKTGMILLCGEITSKAVIDYQRVVRETVKHIGYDDSSKGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VYY0</accession>
    <name>A0A6E8VYY0_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">protein-glutamine gamma-glutamyltransferase</fullName>
            <ecNumber evidence="6">2.3.2.13</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-glutaminyl-[protein] + L-lysyl-[protein] = [protein]-L-lysyl-N(6)-5-L-glutamyl-[protein] + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:54816"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10207"/>
            <dbReference type="Rhea" id="RHEA-COMP:14005"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30011"/>
            <dbReference type="ChEBI" id="CHEBI:138370"/>
            <dbReference type="EC" id="2.3.2.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="9">Binds 1 Ca(2+) ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the transglutaminase superfamily. Transglutaminase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.13" evidence="6"/>
    <dbReference type="RefSeq" id="XP_040232319.2">
        <property type="nucleotide sequence ID" value="XM_040376385.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VYY0"/>
    <dbReference type="EnsemblMetazoa" id="ACON009100-RB">
        <property type="protein sequence ID" value="ACON009100-PB"/>
        <property type="gene ID" value="ACON009100"/>
    </dbReference>
    <dbReference type="GeneID" id="120955483"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009742"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON009100"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042489"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003810">
        <property type="term" value="F:protein-glutamine gamma-glutamyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000090">
        <property type="entry name" value="Protein-glutamine gamma-glutamyltransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.260.10:FF:000001">
        <property type="entry name" value="Protein-glutamine gamma-glutamyltransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000171">
        <property type="entry name" value="protein-glutamine gamma-glutamyltransferase 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002167">
        <property type="entry name" value="Transglutaminase, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.260.10">
        <property type="entry name" value="Transglutaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050779">
        <property type="entry name" value="Transglutaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002931">
        <property type="entry name" value="Transglutaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036985">
        <property type="entry name" value="Transglutaminase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023608">
        <property type="entry name" value="Transglutaminase_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008958">
        <property type="entry name" value="Transglutaminase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036238">
        <property type="entry name" value="Transglutaminase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001102">
        <property type="entry name" value="Transglutaminase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11590">
        <property type="entry name" value="PROTEIN-GLUTAMINE GAMMA-GLUTAMYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11590:SF69">
        <property type="entry name" value="RE08173P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00927">
        <property type="entry name" value="Transglut_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01841">
        <property type="entry name" value="Transglut_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00868">
        <property type="entry name" value="Transglut_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000459">
        <property type="entry name" value="TGM_EBP42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00460">
        <property type="entry name" value="TGc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49309">
        <property type="entry name" value="Transglutaminase, two C-terminal domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4 9" id="KW-0106">Calcium</keyword>
    <keyword evidence="3 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Transglutaminase-like" evidence="10">
        <location>
            <begin position="314"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="322"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="381"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="408"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="448"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="450"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="498"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="503"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00051843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000459-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000459-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009100-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="763" mass="86624" checksum="33703CD94681A4E9" modified="2020-08-12" version="1">MSYLYDYVEKYLPTSWRRKRIRRPIIWSSPEYSPSADVLTVKSVDLCTEENGKDHHTKRYELMSRDEYSGKIPKLVVRRGQPFRLRLVCDRPYDRARDALSLIFTVADEDQPTHGHGTLVGIPVNQFPEQLGEPEEWGAAIEAINGDLLQVLVKPAATAPVGQWKLDIDTKLLSEAFGKSYSLPQAFYVLFNPWCPDDLVYMEEKAHRHEYVMSDTTLIYRGSYNRLRPSVWKFGQFEKHVLDCSLLLIAKIGKVSATHRGDPVRVCRAISAAVNSPDDDGALMGNWSGDFSGGTPPTKWVGSVEILQQFYKRQKPVKFAQCWVFAGVVSTICRAIGIPSRVVTNYSSAHDTQASLTVDYFVDKSGKIMEEMNADSIWNYHVWNEVWMQRPDLGISSDGDYGGWQAIDATPQESSDGMFRCGPASVLAVKLGEVLKPYDNNFLFAEVNADKVFWRYTGPHHPLKLLRKDVLGIGLFISTKAVGRWEREDITSSYKFAEKSEEERATMLKALKQANSYFSRYYLNEEFNEVYFNFELRDDIKIGEPFSVILTVKNRSSENRHVVEGSLHVDTVLYTGKDRESVKVDKFSVTLEPGTEESIRMIVEFHEYYRKLRDQAAFNISCMATVLDTEFEFYAQDDFRVRKPDIKISLLGKPVSQAPVDVQFTLENPLPIPLHKGLFHLEGSGIGKPLLFKHAEIAPGEKVSNTFTMTPPYSGRLTLAAKFTSKELDDVDGFLAFLAHPRPEDIITEVEDNVIVARTDVID</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VYY6</accession>
    <name>A0A6E8VYY6_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CAP-Gly domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON009210-RA">
        <property type="protein sequence ID" value="ACON009210-PA"/>
        <property type="gene ID" value="ACON009210"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005722"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON009210"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041357"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.190">
        <property type="entry name" value="CAP Gly-rich-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036859">
        <property type="entry name" value="CAP-Gly_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000938">
        <property type="entry name" value="CAP-Gly_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032108">
        <property type="entry name" value="CLIP1_ZNF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18916">
        <property type="entry name" value="DYNACTIN 1-RELATED MICROTUBULE-BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18916:SF93">
        <property type="entry name" value="RESTIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01302">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16641">
        <property type="entry name" value="CLIP1_ZNF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01052">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74924">
        <property type="entry name" value="Cap-Gly domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00845">
        <property type="entry name" value="CAP_GLY_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50245">
        <property type="entry name" value="CAP_GLY_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0493">Microtubule</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CAP-Gly" evidence="9">
        <location>
            <begin position="48"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="CAP-Gly" evidence="9">
        <location>
            <begin position="177"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="98"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1124"/>
            <end position="1145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1426"/>
            <end position="1468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1696"/>
            <end position="1726"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="289"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="109"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1448"/>
            <end position="1468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1709"/>
            <end position="1726"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009210-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1747" mass="195862" checksum="629CD0E5774E1CAE" modified="2020-08-12" version="1">MRRPSDSDYNKTHLPDQGAVLTADTDSFIIGQRVWVGGIRPGQIAYIGETHFAPGEWAGVVLDEPNGKNDGSVAGKRYFQCEAKKGVFSRLTRLTREPLANAGGGGGDSTASTPLDASFRSLTSPARSGTVSPTHSVQSYASKSPAMAGKAASLNVGDRVIVSSGFGSRPGMLKFIGETQFASGTWCGVQLDEASGKNDGTVDGVRYFECPAKYGIFVPIAKVTLSPSARKTSARLSRANSKESLNSLATMSSIATTATSRLRMSAQRKSTVSAVPSTPKSSFSLQDVLREKQNHIEQLMQEREIDREETANQSIMYQKNIQQLKEKVTLLEKSLADEKRRNEDLQFSVDEATFCGEELNAQTQVFKEKIADLEAQLAAGGTAGKPNEPTQPGVPPEEINELKRSLENEIQNLHEKVADAERAAASKLSALTISEECLKEQINYLEHRVDEQSDQLTVKDAELEKQYLALKNAESEQEERLAALQDELRSKKDLLAERDQQVQLLEQTVEELRADVRQRDLKTVELESDLRAKLQEQGTSSSKLAEKLSELEVALVEVNASKTHLERDLTNATQTIKVLEEDRSVKEKAAQEVESKLTASEAALKAEIASRQEQESLAQKLQRDLQSLATSGESSAALLAAKQDELSNQAKQLQELEAEKVKVQQELSSLQQKFEQSRTEHEQLIAEVHALADAERNTIAELRKQLQTSEQENLAKDKQLEENEVLVSALQNELKELNVSKASLNQELTAIKASFADKDGTLANILQEKTALEKQLEESKQELASKVKQLEEDLRNREDTLRKELELSASTAQQQLSAKEEELTRLSQAREELQKQLEVAQQQMKDVSDKMKQAEDTIATQTNESQSLNQQLSSLRSELSSKDEQLAKLNAVLAETAAREEAGGKKLGEAAEQYGKLEIEHADLRRKMDALEQKSAQVQQQKLDLERELDLQRSSTLDSNSELAKLTDELKAKQRALDELRDSYDTLKIETERRADESAQANAALQEQVERVRQEMQQISDQKIIQENELNTELRKIKELAEQEQDNLVRTIAELKVSFEQERQRIDSEHAAELAKSVSEREQQMAAMKSELESALGELKAQLEDARTESKKLQELNETAVRAAKEQEESLQKQLQQSRDESSTLQQRLDELRQSMEQGSQDLTVQIDQKAQRIVELEQELDEQRTLQQKRSAEVAEMVAKLEENGKSYAEMLQQLQDSYTQIEALKKAKSESEEACQQVQQRLQDLNSSYSEMEEEQVDLVSREETLRKELALLQEQMQQAASEQKERYDAVVSKNEELLKQLESTSSAKGATETELIALRQELATKSTSLGELHANVEELNAQLQTKATLEQQVQSLEQSVSAKDASILELSGKVEDLQRQTTSSDAKIVEKEEELKQLQTASASKDTQLKDLQQQLEAMQKTLTDSTELSKRTSAEATELQAALEKSRTTVKEQEDRQKEQQRRIAELETKLAAQATQFDELLDRKKSAETEYSHRTHDLSQKLLELESAKKQEIDELQQRLAELMQRVETQVSETAQTVSSKRAVEKRQHELECAKKDLELRETELQLANRRLEKDNEQLRSQLVLKESELTKLAKAATAAATAADNSGSVPNRGVRVMSESKEDDSGAQIDFLNSIIVDMQRKNEKLTLRIQALESTSADSHNMSFDIQKRKPAPRVFCDICDEFDLHETEDCPKQCSDSPPESLKHPSDGKERKEPPPRKYCESCEVFGHEIGECPDDETY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VZ94</accession>
    <name>A0A6E8VZ94_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ATP synthase F1 subunit epsilon</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ATPase epsilon family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040234800.1">
        <property type="nucleotide sequence ID" value="XM_040378866.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_049465221.1">
        <property type="nucleotide sequence ID" value="XM_049609264.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VZ94"/>
    <dbReference type="EnsemblMetazoa" id="ACON009564-RB">
        <property type="protein sequence ID" value="ACON009564-PB"/>
        <property type="gene ID" value="ACON009564"/>
    </dbReference>
    <dbReference type="GeneID" id="120956982"/>
    <dbReference type="CTD" id="44046"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012360"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON009564"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041617"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042776">
        <property type="term" value="P:proton motive force-driven mitochondrial ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12153">
        <property type="entry name" value="F1-ATPase_epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1620.20:FF:000005">
        <property type="entry name" value="Uncharacterized protein, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1620.20">
        <property type="entry name" value="ATP synthase, F1 complex, epsilon subunit superfamily, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006721">
        <property type="entry name" value="ATP_synth_F1_esu_mt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036742">
        <property type="entry name" value="ATP_synth_F1_esu_sf_mt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12448">
        <property type="entry name" value="ATP SYNTHASE EPSILON CHAIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12448:SF0">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT EPSILON, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04627">
        <property type="entry name" value="ATP-synt_Eps"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48690">
        <property type="entry name" value="Epsilon subunit of mitochondrial F1F0-ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009564-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="56" mass="6483" checksum="B3517B43B44D5A60" modified="2023-06-28" version="2">MAAWRTAGLNYINYSNIAARLLRKALKPELRVQAARRDDSHIKFTKWQGGKPENEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W0D0</accession>
    <name>A0A6E8W0D0_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="10">BZIP domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bZIP family. Maf subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040232190.2">
        <property type="nucleotide sequence ID" value="XM_040376256.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8W0D0"/>
    <dbReference type="EnsemblMetazoa" id="ACON010405-RB">
        <property type="protein sequence ID" value="ACON010405-PB"/>
        <property type="gene ID" value="ACON010405"/>
    </dbReference>
    <dbReference type="GeneID" id="120955407"/>
    <dbReference type="CTD" id="37336"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006800"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON010405"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039311"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14717">
        <property type="entry name" value="bZIP_Maf_small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000011">
        <property type="entry name" value="Transcription factor MafG, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004826">
        <property type="entry name" value="bZIP_Maf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008917">
        <property type="entry name" value="TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024874">
        <property type="entry name" value="Transcription_factor_Maf_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10129:SF48">
        <property type="entry name" value="MAF-S, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10129">
        <property type="entry name" value="TRANSCRIPTION FACTOR MAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03131">
        <property type="entry name" value="bZIP_Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47454">
        <property type="entry name" value="A DNA-binding domain in eukaryotic transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="9">
        <location>
            <begin position="53"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010405-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="134" mass="15902" checksum="4602681802E5A19F" modified="2020-08-12" version="1">MPQEIKRERKTAQAPLSPCPIPDISDDELVSITVRDLNRTLKMRGLTREEIVRMKQRRRTLKNRGYAASCRIKRIEQKDELETEKSQEWRDMELMHEETGRLQEENDSLRNKYEALRKFALSKKIPLPPELDVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W0D3</accession>
    <name>A0A6E8W0D3_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">DNA-(apurinic or apyrimidinic site) endonuclease</fullName>
            <ecNumber evidence="14">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.</text>
            <dbReference type="EC" id="3.1.11.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12 14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="12 14">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="12 14">Probably binds two magnesium or manganese ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the DNA repair enzymes AP/ExoA family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="14"/>
    <dbReference type="RefSeq" id="XP_040237101.2">
        <property type="nucleotide sequence ID" value="XM_040381167.2"/>
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        <property type="protein sequence ID" value="ACON009587-PA"/>
        <property type="gene ID" value="ACON009587"/>
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    <dbReference type="GeneID" id="120958396"/>
    <dbReference type="CTD" id="8568"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_013202"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON009587"/>
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        <property type="component" value="Unplaced"/>
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        <property type="term" value="C:nucleus"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:DNA binding"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
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        <property type="term" value="F:double-stranded DNA 3'-5' DNA exonuclease activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:phosphoric diester hydrolase activity"/>
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        <property type="term" value="P:base-excision repair"/>
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        <property type="entry name" value="DNA-(apurinic or apyrimidinic site) lyase"/>
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        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
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        <property type="entry name" value="exoDNase_III"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="xth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748">
        <property type="entry name" value="AP ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748:SF6">
        <property type="entry name" value="DNA-(APURINIC OR APYRIMIDINIC SITE) ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AP_NUCLEASE_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00727">
        <property type="entry name" value="AP_NUCLEASE_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51435">
        <property type="entry name" value="AP_NUCLEASE_F1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 14" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9 14" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 12 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12" id="KW-0464">Manganese</keyword>
    <keyword evidence="5 12 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="16">
        <location>
            <begin position="341"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="15"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="136"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="177"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="196"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="217"/>
            <end position="231"/>
        </location>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="289"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="443"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="11">
        <location>
            <position position="482"/>
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    <feature type="active site" description="Proton acceptor" evidence="11">
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    <feature type="binding site" evidence="12">
        <location>
            <position position="343"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
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        <location>
            <position position="371"/>
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        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
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        <location>
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        <ligand>
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        <ligand>
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        <location>
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        </location>
    </feature>
    <feature type="site" description="Important for catalytic activity" evidence="13">
        <location>
            <position position="554"/>
        </location>
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    <feature type="site" description="Interaction with DNA substrate" evidence="13">
        <location>
            <position position="580"/>
        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00000493"/>
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        <source>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
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        <source>
            <dbReference type="Pfam" id="PF03372"/>
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    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="589" mass="64853" checksum="ABC580E16C791E16" modified="2023-06-28" version="2">MEEKPKKRGRQPRGAAKEAAVEAEKQQEQLTEPEQMPAEPLKARGGSKRKAIVPEPAPVVEKPARKPAAKRTKAADVVEESNVVETNGAKTNGEVMEKVAPAKKGRSTKKETVPEQEASSNGTVEDGKAKGKKAPAKRETKATKASKTVEEPAAVKEMVEEEEAPAPAKRGRTKASTKQEEESEKAAPAKPKGRGKAKDKTDGGYDVSETKPVEPEASSAVKSTAKTAKPTTKGRKKAAEDTAAADNGEALEDAPKKATKKKAPSEEATNGDIPTVTQRKRAKPQPPADEMEEDEEEAVPSKGKKAKTASTAPKTNISATNYGKLNFALEEDKQWNFKISSWNVAGLRSWVGKGGLEFIEHEKPDILCVQETKCTEDQMPDEARHIKGYHPYWLCKPGGHAGVAIYSKKMPFHVAYGLGDEEQDQDGRLLTAEYEKFYLVCVYVPNAGRKLVTLPKRLRWNEKFHQYLRDLDAKKPVILCGDMNVAHEEIDLANPKTNKKNAGFTPEEREGMTELLSYGFVDTFRKLYPDRTGAYTFWTYMGGARAKNVGWRLDYFITSERLAGKVTDNVIRSQVFGSDHCPVTLFLNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W2F5</accession>
    <name>A0A6E8W2F5_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">L antigen family member 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. LAGE3 functions as a dimerization module for the complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CTAG/PCC1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040234859.1">
        <property type="nucleotide sequence ID" value="XM_040378925.2"/>
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        <property type="protein sequence ID" value="ACON011310-PA"/>
        <property type="gene ID" value="ACON011310"/>
    </dbReference>
    <dbReference type="GeneID" id="120957019"/>
    <dbReference type="KEGG" id="acoz:120957019"/>
    <dbReference type="CTD" id="8674046"/>
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    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040894"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000408">
        <property type="term" value="C:EKC/KEOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070525">
        <property type="term" value="P:tRNA threonylcarbamoyladenosine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.50:FF:000005">
        <property type="entry name" value="L antigen family member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.50">
        <property type="entry name" value="Alpha-D-phosphohexomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015419">
        <property type="entry name" value="CTAG/Pcc1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31283">
        <property type="entry name" value="EKC/KEOPS COMPLEX SUBUNIT PCC1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31283:SF5">
        <property type="entry name" value="EKC_KEOPS COMPLEX SUBUNIT LAGE3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09341">
        <property type="entry name" value="Pcc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00053047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00076355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON011310-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="94" mass="10693" checksum="BADD0EC6F4F15D11" modified="2020-08-12" version="1">MCAAANIEVSIKIPFPTKREAEIAYDVLRVDSEPKRSFVHKTITLEENHLLLRLGGEQAKNVRVGVTSFCELLLLCCETLDQFGPPKSDRYEHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W2H1</accession>
    <name>A0A6E8W2H1_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Elongator complex protein 4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="3">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ELP4 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8W2H1"/>
    <dbReference type="EnsemblMetazoa" id="ACON010407-RA">
        <property type="protein sequence ID" value="ACON010407-PA"/>
        <property type="gene ID" value="ACON010407"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_013393"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON010407"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041261"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033588">
        <property type="term" value="C:elongator holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008023">
        <property type="term" value="C:transcription elongation factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19494">
        <property type="entry name" value="Elp4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008728">
        <property type="entry name" value="Elongator_complex_protein_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12896:SF1">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12896">
        <property type="entry name" value="PAX6 NEIGHBOR PROTEIN PAXNEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05625">
        <property type="entry name" value="PAXNEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0819">tRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="254"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="259"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010407-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="279" mass="31343" checksum="74382BEDD8E3322C" modified="2020-08-12" version="1">INKLPTPLEESKTADNNSTMPEPETMRIAFRYNQLTPVDSEQKPATSLGHYFDLTKTMSPDMLLAHDVTCWNGGELETNAPEFGSFNNPHYASLLSCIRSKAGEQQFNAASDESSKNVLRICLNSMGSPLWYGKQFSEDFLRFMVLLKAIVRNTLSVCLITVPLHLFNHLDDVYLSKKVRNMFDFSFDLEAFAGQMEEQANPAFKEYHGLLNITKITPFNSLASYHPKTRDLAFKLKRSKFVIEKLHLPPDIADEDGASKSQVPTMSCASVGGKSKLDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W2H9</accession>
    <name>A0A6E8W2H9_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Receptor expression-enhancing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8W2H9"/>
    <dbReference type="EnsemblMetazoa" id="ACON011331-RD">
        <property type="protein sequence ID" value="ACON011331-PD"/>
        <property type="gene ID" value="ACON011331"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000364"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON011331"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042433"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005881">
        <property type="term" value="C:cytoplasmic microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071782">
        <property type="term" value="C:endoplasmic reticulum tubular network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071786">
        <property type="term" value="P:endoplasmic reticulum tubular network organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004345">
        <property type="entry name" value="TB2_DP1_HVA22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12300">
        <property type="entry name" value="HVA22-LIKE PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12300:SF117">
        <property type="entry name" value="LP05237P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03134">
        <property type="entry name" value="TB2_DP1_HVA22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="68"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="209"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="345"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="220"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="273"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="356"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="393"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON011331-PD"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="431" mass="46039" checksum="F978FDA04BD2289B" modified="2020-08-12" version="1">MLSRIPFPRMSVTGMGLCSLVAIFFCPRAIGSNILFPGFRLLFGTLYPAYASYKAVRTKNVKEYVKWMMYWIVFAFFTFIETFTDILLSWFPFYYEIKVIVVLWLLSPATRGSSTLYRKFVHPMLTRREQEIDDYINQAKEKGYTAVLQLGSKGVNYATNVIMQTAIKVLGTKTDTNTPFGSMMRISQSDNSLLMSAAAAAAVRASGAEPARPNGLLHTNMRDTTDHATSGEDEHDGQMVTAATSGPATGRVRRSQSVDSALNSRGGGGGGRATMSSSRSMTRARAQQQQQPAIIYEMDSEDEERFLASETDLILGITSSATTTASSAAGGGIIRGRTRAITTVSAEEESDGTEGGSVARASASTRAKATTAKETGRAKKSTKAVGVTGGKAGTKASSTAAAGGRRKVNGVGAAAAAEMESVEMGVEMDVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W2I6</accession>
    <name>A0A6E8W2I6_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein-serine/threonine kinase</fullName>
            <ecNumber evidence="10">2.7.11.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[pyruvate dehydrogenase E1 alpha subunit] + ATP = O-phospho-L-seryl-[pyruvate dehydrogenase E1 alpha subunit] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23052"/>
            <dbReference type="Rhea" id="RHEA-COMP:13689"/>
            <dbReference type="Rhea" id="RHEA-COMP:13690"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the PDK/BCKDK protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.-" evidence="10"/>
    <dbReference type="RefSeq" id="XP_040239845.1">
        <property type="nucleotide sequence ID" value="XM_040383911.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8W2I6"/>
    <dbReference type="EnsemblMetazoa" id="ACON010276-RA">
        <property type="protein sequence ID" value="ACON010276-PA"/>
        <property type="gene ID" value="ACON010276"/>
    </dbReference>
    <dbReference type="GeneID" id="120960033"/>
    <dbReference type="CTD" id="35970"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011549"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON010276"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042511"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004740">
        <property type="term" value="F:pyruvate dehydrogenase (acetyl-transferring) kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010906">
        <property type="term" value="P:regulation of glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16929">
        <property type="entry name" value="HATPase_PDK-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000007">
        <property type="entry name" value="Mitochondrial pyruvate dehydrogenase kinase isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.20">
        <property type="entry name" value="Alpha-ketoacid/pyruvate dehydrogenase kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036784">
        <property type="entry name" value="AK/P_DHK_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018955">
        <property type="entry name" value="BCDHK/PDK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039028">
        <property type="entry name" value="BCKD/PDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11947:SF3">
        <property type="entry name" value="[PYRUVATE DEHYDROGENASE (ACETYL-TRANSFERRING)] KINASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11947">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10436">
        <property type="entry name" value="BCDHK_Adom3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69012">
        <property type="entry name" value="alpha-ketoacid dehydrogenase kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Histidine kinase" evidence="11">
        <location>
            <begin position="235"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU366032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010276-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="399" mass="45645" checksum="D723F82C03A8DB94" modified="2020-08-12" version="1">MKLFPVRLSNINKMLDFYSQFNPSPLSIKQFIDFGLNACPRKSFVFLRKELPVRLANIMKEITLLPESLLRMPSVGLVSAWYVKSFEEVLAFEKTDPTENNLEKFCKSLTQIRDRHSDVVQTMAQGILELKESRDGAIEPSTELSIQYFLDRLYMSRISIRMLINQHTILFGDIPQTGRHIGSIDPLCDPHMVVRDAYENARFLCDQYYLASPELEVIEHNEIDKGNPIKIVYVPSHLYHMLFELFKNSMRAVMEHHGTENDVPPIKVTIVKGKEDICVKMSDQGGGIPRSQVDQLFKYMYSTAPQPPKSKTDLPLVPLAGYGYGLPISRLYARYFHGDLVLFSCEGYGSDAIIYLKAFSDEANELLPIFNKTSTRFYKATVPTGDWSNQGKNFTNRLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W729</accession>
    <name>A0A6E8W729_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="9">TMC domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMC family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8W729"/>
    <dbReference type="EnsemblMetazoa" id="ACON012019-RA">
        <property type="protein sequence ID" value="ACON012019-PA"/>
        <property type="gene ID" value="ACON012019"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_003823"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_013223"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON012019"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030626"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042609"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008381">
        <property type="term" value="F:mechanosensitive monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038900">
        <property type="entry name" value="TMC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012496">
        <property type="entry name" value="TMC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23302:SF24">
        <property type="entry name" value="TMC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23302">
        <property type="entry name" value="TRANSMEMBRANE CHANNEL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07810">
        <property type="entry name" value="TMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="163"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
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            <begin position="255"/>
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    <feature type="transmembrane region" description="Helical" evidence="7">
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            <end position="398"/>
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            <begin position="418"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
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            <begin position="448"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
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            <begin position="506"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="569"/>
            <end position="588"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="612"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="668"/>
            <end position="693"/>
        </location>
    </feature>
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            <begin position="497"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
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            <begin position="738"/>
            <end position="811"/>
        </location>
    </feature>
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        <location>
            <begin position="775"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="798"/>
            <end position="811"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006510"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF07810"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblMetazoa" id="ACON012019-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="811" mass="91293" checksum="BF3342B053556981" modified="2020-08-12" version="1">MSLKTIEGNFGTGVVAYFLFLRWLVFLNLLLFLLIFAFVVLPHLVLREPTDLPCDLVTDPTSVQCCSESYENVTRTAQFSVLDLIQGTGFVEGTLLFYGMYTNQIYGYSPMEDRAYHTARQLRMSAAAAAAINGSTVETSRLLESDLPDPERQTILYYDLPLVYVIIIAIYYIITLVAIMRAVVRQFKDRIAEGEGLFYQYCNLVFGGWDFCIHNQKSADIKHRALHNEIKSLLYQKRFEHERNNRSREFMLKLIAIRMLVNLVVFVILLLAAITIYVLFNVSLAELEPNFTPSHPVSFSEGIGNRTLYMSWPSSWSVFSSASQRGAISFASTITPLLSSISDEVGAGGGGSGGFNATDPTMERLSLEEKLRVLFYEFLPYLAIVCMNLVVPQLFNYLVQYEKYSPLFVIKISLFRTVFLRLSSLAVLLSRFYFLITPMNLPEANASLVANLTASTISVLGTLLLDRQSPVSEAQTQQQQLQLPTQCYDEQNGAPQCWETFVGQQFYKLFIVDFATHFLVTFFVNFPRALFARHSSSRLAKFIGEQEFELSKHVLDVIYSQTLCWLGTFYAPFLPAIAALLTFLMFYIKKFACLVNSNPSAILYRASRSKSLFMSTLLISFTLALVPVVYALAEIVPSRSCGPFRGLPSVWDRAIAAFMKMPQFFQNVIFYFGTASFAIPCFVVLTFFIYYYYAVSTANRHMVQVLKQQLVLEGHDKQFLLSRLSLFIKQQQDNTANIVIPPPSAYQQQQPPVVPPPRTNSQSQASEPTPALPPRADRDSKPSSRDRPKDLPPPPIPASGSSSTGNTTGAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W8W7</accession>
    <name>A0A6E8W8W7_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8W8W7"/>
    <dbReference type="EnsemblMetazoa" id="ACON013481-RG">
        <property type="protein sequence ID" value="ACON013481-PG"/>
        <property type="gene ID" value="ACON013481"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACON013481"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON013481-PG"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="156" mass="18301" checksum="3D8AAAD497CF119E" modified="2020-08-12" version="1">MVYESDFYSTRRVGSSYTRPTISSYTVTRRGVDWDKVPFVPRPSLIPDPVTAYGKRQPRKEARVSILEAINREGIEPDPRILARPIDQYRSPRDLNRQRIANELHRREYNRATGHTTDADNVDTLLRRVHGTAPVKEGRRHVMFFEPGYARTYYRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W942</accession>
    <name>A0A6E8W942_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8W942"/>
    <dbReference type="EnsemblMetazoa" id="ACON028559-RA">
        <property type="protein sequence ID" value="ACON028559-PA"/>
        <property type="gene ID" value="ACON028559"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012535"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON028559"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039426"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="182"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="98"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="123"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="188"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="204"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON028559-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="242" mass="26633" checksum="235D18FB76A5E65F" modified="2020-08-12" version="1">MLSPSAHSHHGQTGFSRWSTSHLNSNQTSPSVSTRGSFTRGGLNASVISGYSTASAATGANHGNGSSTIRNSFRQPYLGWRSQEKLSQPRTPAERLASSLLQQQQGSSKQKDHQQQQQQSNEPKGKQQQQKDESVVTPEIQSSIIEVTSAIVHYVNDQTNRHSRSRSTSPSQRCWLESSFVGTRPLDSPQTPLIENSSVLGSGHQQQQQQQHQHQQQQPHTAGDHYRFNAGRMNGVGESTLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W969</accession>
    <name>A0A6E8W969_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8W969"/>
    <dbReference type="EnsemblMetazoa" id="ACON013755-RB">
        <property type="protein sequence ID" value="ACON013755-PB"/>
        <property type="gene ID" value="ACON013755"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_003680"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON013755"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032421"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="90"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON013755-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="227" mass="25616" checksum="3843C9DA31E22EF0" modified="2020-08-12" version="1">MVIISVPQRVISMPCTSDSDGQTVNTIRTDRPTVAGSKSMGVPVAAPTVASSTPLAIKKDLFMSRGWGASGMPFSMFYLNHYTKAQKAYAQNQLHQQQQHQQQQEQLQQQQRQQQLLQLQKQQLEEQQLKQQQLLRIQQQQDEHSLVDYQEDATTKGVGYPLVPKTLPFGARYPLDPEPIAVHAQQLRTDTDYVDSASSVRTSKVNTARRQYTVPQLFVSYGWGPMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WAC4</accession>
    <name>A0A6E8WAC4_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="3">PlsC domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8WAC4"/>
    <dbReference type="EnsemblMetazoa" id="ACON013284-RA">
        <property type="protein sequence ID" value="ACON013284-PA"/>
        <property type="gene ID" value="ACON013284"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_008443"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON013284"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_036788"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07987">
        <property type="entry name" value="LPLAT_MGAT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22753:SF14">
        <property type="entry name" value="MONOACYLGLYCEROL_DIACYLGLYCEROL O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22753">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 68"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="2">
        <location>
            <begin position="96"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON013284-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="326" mass="37049" checksum="822449609B7FAEC8" modified="2020-08-12" version="1">MDNESISHASGGLGSTLIVPNWFGWMHNLSLPLLVVFLLPWMYVLLILSSIPYLHLCNFLRLRFWKQSNEDEFWKAARWLMAYVWNLHSKLFHGYEVTGLEHLPETGGGALLIYYHGALPIDMYYLTAETMLKCNRLIHTVGDRFLDRIPGWRLVSRVMNVTSGSVQSCVATLRAGELLSIAPGGVYEAQFGDSVYEVLWKNRTGFARVALEAKVPIIPMFTVNIRECFRTVSFAKWLFVRLYSVLKVPVLPIYGGFPVKLRTVLGKPIPYDESLSPVALQERVAAAIAELVREHQRVPGDILQAIGDRFETVHAVEQREEEDTKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WAD0</accession>
    <name>A0A6E8WAD0_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="4">DUF4758 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8WAD0"/>
    <dbReference type="EnsemblMetazoa" id="ACON029559-RA">
        <property type="protein sequence ID" value="ACON029559-PA"/>
        <property type="gene ID" value="ACON029559"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002507"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON029559"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037153"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd00037">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050111">
        <property type="entry name" value="C-type_lectin/snaclec_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22803">
        <property type="entry name" value="MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
        <property type="entry name" value="C_TYPE_LECTIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026231834" evidence="2">
        <location>
            <begin position="35"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="3">
        <location>
            <begin position="67"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON029559-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="195" mass="22596" checksum="BF142681BC2B5FD5" modified="2020-08-12" version="1">MQPNKLVSHCVRWNRMGFVLGVLVLLLSESVIQCATPKALAGNSHDCPNCVEESSAPPRWTMPLLKLGEKRYYLSIFFKANWFKALQYCRFHGMQLASIQTQEENDRLEKYVKDYGLATEHFWTSGTDLAEEGSFFWVSNGRPLSFTNWNAGEPNNFRYENGEEEHCLELWNRDGKGLKWNDTPCSFETYFICEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WCG6</accession>
    <name>A0A6E8WCG6_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Myosin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040230778.2">
        <property type="nucleotide sequence ID" value="XM_040374844.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040230779.2">
        <property type="nucleotide sequence ID" value="XM_040374845.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8WCG6"/>
    <dbReference type="EnsemblMetazoa" id="ACON029606-RB">
        <property type="protein sequence ID" value="ACON029606-PB"/>
        <property type="gene ID" value="ACON029606"/>
    </dbReference>
    <dbReference type="GeneID" id="120954694"/>
    <dbReference type="KEGG" id="acoz:120954694"/>
    <dbReference type="CTD" id="38153"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_008528"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON029606"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032917"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045177">
        <property type="term" value="C:apical part of cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005902">
        <property type="term" value="C:microvillus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030048">
        <property type="term" value="P:actin filament-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007368">
        <property type="term" value="P:determination of left/right symmetry"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048803">
        <property type="term" value="P:imaginal disc-derived male genitalia morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007498">
        <property type="term" value="P:mesoderm development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01378">
        <property type="entry name" value="MYSc_Myo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.820:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000101">
        <property type="entry name" value="Slow myosin heavy chain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.530:FF:000004">
        <property type="entry name" value="Unconventional myosin ID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010926">
        <property type="entry name" value="Myosin_TH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036072">
        <property type="entry name" value="MYSc_Myo1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140">
        <property type="entry name" value="MYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140:SF679">
        <property type="entry name" value="UNCONVENTIONAL MYOSIN IC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06017">
        <property type="entry name" value="Myosin_TH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51757">
        <property type="entry name" value="TH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="5 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="8 10" id="KW-0505">Motor protein</keyword>
    <keyword evidence="7 10" id="KW-0518">Myosin</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Myosin motor" evidence="11">
        <location>
            <begin position="12"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="domain" description="TH1" evidence="12">
        <location>
            <begin position="845"/>
            <end position="1024"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="10">
        <location>
            <begin position="568"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="105"/>
            <end position="112"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON029606-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1024" mass="118086" checksum="3C0963FF25C33AC5" modified="2020-08-12" version="1">MERGLHDRDRVGLQDQVLLEDYQSEDAFIDNLRKRFQENLIYTYIGHVLISVNPYKELPIYTESDVKEYRKRHFFEAPPHVFALSDNAYRSLTEENRGQCILISGESGSGKTEASKKVLQFIAAATGHTQNVEGVKDKLLQSNPVLEAFGNAKTNRNDNSSRFGKYMDVQFDFAGVPEGGNILNYLLEKSRVIHQSGGERNFHIFYQLLAGADDNLLRELHLKRNLDTFYYLSDGANGNVPNIRDADNFRMVQKAMTVIEIPDQEQRQILDIVAAVLHMGNVGFTEEEGKAKILKPESVTAIAKLLGCNEEQLTSAFTHRTIEARGEIVTSPLNRDLAIYARDALAKAVYDRLFTWLVSRINTSLHAADLPKKNSVMGILDIYGFEIFKKNSFEQFCINFCNEKLQQLFIELTLKQEQEEYLREGIEWVPVEYFDNKVICNLIEEKHKGIVALMDEECLRPGDPTDLSFLAKMNDNLGSHPHYICHSRASTTVQKTMGRDEFRLVHYAGDVTYSVHGFLDKNNDLLFRDLKETMAECSNGIIRSCFPAADIGSKRRPETAVTQFKNSLNNLMDILMCKEPSYIRCIKPNDLQTHGQFDVELVRHQVKYLGLMENLRVRRAGFAYRRTYELFLQRYKCLSRQTWPHYHGPAQDGVQILANELGYGKDDYRMGKTKIFIRFPKTLFDTEDAFQARKHYLASIIQARWKGRRQRKAYLEIRRKIIVGQTYIRRYLAVQTLKRRRDAAAKIRFFIKGFITRHDEPNECNRSFIQLTKRHWLLKLAKSLPTSFMNHTWIAAPKHCQDASILLRQMHKLHLARCYRVALNAERKRQLDLKVLSEAVFKNSKRNYPESVGPWFVDDRIAKQHATQISNFTVTQMNGEKLHYSTPVVKYDRRGYKPRERFFLLSNKAVYLLDGKTYKQKHRLPLDKVDFCITNERDGIMLIRIPLELKKDKGDLILDVPDIIECCIWILDATKNRSIINIVDTGSLSHNLVRGKTGVIEIQTGPQPSITRAKSGNLLVIAGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WCH3</accession>
    <name>A0A6E8WCH3_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="5">BTB domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_040225011.2">
        <property type="nucleotide sequence ID" value="XM_040369077.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8WCH3"/>
    <dbReference type="EnsemblMetazoa" id="ACON029614-RA">
        <property type="protein sequence ID" value="ACON029614-PA"/>
        <property type="gene ID" value="ACON029614"/>
    </dbReference>
    <dbReference type="GeneID" id="120950781"/>
    <dbReference type="CTD" id="42089"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004255"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON029614"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038130"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022008">
        <property type="term" value="P:neurogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18488">
        <property type="entry name" value="BACK_BTBD3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18282">
        <property type="entry name" value="BTB_POZ_BTBD3_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.420:FF:000003">
        <property type="entry name" value="BTB/POZ domain-containing protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.820:FF:000001">
        <property type="entry name" value="BTB/POZ domain-containing protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000015">
        <property type="entry name" value="BTB/POZ domain-containing protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.820">
        <property type="entry name" value="PHR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049737">
        <property type="entry name" value="Btbd6a-like_BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012983">
        <property type="entry name" value="PHR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038648">
        <property type="entry name" value="PHR_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45774">
        <property type="entry name" value="BTB/POZ DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45774:SF9">
        <property type="entry name" value="LUTE, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08005">
        <property type="entry name" value="PHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="4">
        <location>
            <begin position="144"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="567"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="582"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="611"/>
            <end position="622"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON029614-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="686" mass="73042" checksum="7EE6E762A6457220" modified="2020-08-12" version="1">MSNLYAKISQRPMKRNEEQIFSVFPYHNIEMFNVLAPFHQNSAGAAGAGVAAAAAGGQNVRLENMQVTQPCSGPSSPTIASPGALSSTSFCLANGGGIGSVLGSGGCSVISSTTAADTADPNWQANKSTVRERNAAMFNNDLMADIRFIVGSDEQVQTIPAHKYVLATGSSVFYAMFYGGLAENKQEIKVPDVEPGAFLTLLKYLYCDEIQLEADNVLATLYVAKKYIVPHLARACVNYLETSLTAKNACLLLSQSRLFEEPELMQRCWEVIDAQAEMAIKSEGFVDIDLKTFETILARETLNCKEIHLFEAALSWAHAACTKMDIEPTSSNKRQLLGQALYLIRIPTMTLEEFANRVAQLGILTNQETIDIFLNFTAKNKPKLTFPVKARAGLKTQVCHRFASCAYRSNQWRYRGRCDSIQFSVDKRIFIVGFGLYGSSTGAADYDVKIELKRLGRVLAENSTKFFSDGSSNTFQVFFETPIQIEPECFYTASVVLDGTELSFFGQEGMSEVSVGTVTFQFQCSSESTNGTGVQGGQIPELIFYGPMGVSQQSSIISASASNNTINNNHTAAGGHTAGKQPSNNSGGSSSNSVPSTTTTSSSSSSSSSAHGGGLLLMGAGGSSSPSNTHSSDRSNVAPFGNGGDNDGASTASANAAGIANWLPPSVAGVSAGQQQLLAGTGEPTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9I3BD90</accession>
    <name>A0A9I3BD90_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2023-03" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A9I3BD90"/>
    <dbReference type="EnsemblMetazoa" id="ACON030710-RD">
        <property type="protein sequence ID" value="ACON030710-PD"/>
        <property type="gene ID" value="ACON030710"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_036950"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="843"/>
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        </location>
    </feature>
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        <location>
            <begin position="880"/>
            <end position="916"/>
        </location>
    </feature>
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        </location>
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            <begin position="87"/>
            <end position="108"/>
        </location>
    </feature>
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            <begin position="127"/>
            <end position="139"/>
        </location>
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            <begin position="152"/>
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            <begin position="180"/>
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        </location>
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        <location>
            <begin position="216"/>
            <end position="228"/>
        </location>
    </feature>
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        <location>
            <begin position="257"/>
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        </location>
    </feature>
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        <location>
            <begin position="330"/>
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        </location>
    </feature>
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            <begin position="349"/>
            <end position="362"/>
        </location>
    </feature>
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
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    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON030710-PD"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1049" mass="112124" checksum="6EBEBB2619434A7C" modified="2023-06-28" version="1">MDRSSYGGPPQSTIGNSARRIQAQLPAEPPACVQNAMMRRDKQPFTYTPGGIDLSQIKSPRMAKRISRNANSEGVTGQPKVSPLAQNNGSSYSSNGTSSSNGNTSSVSPAATLGAAAMGMPFQVFPTGPPAPPPPPPPTGRVLPSNGGNRSIPPPPPPPGPAQPTASSSDARKSPRPQSFEPPPMGMRPEIKIPPNPMAALRKAPRPQPKNDFWIEEYRREKQQDERPQQIPRPVPPSYSYTNRDLDDEVAKNTFNRTDELTVRGDHPLAQHHHRSPSPSNLTVPHESGSRGSSSIKTPPVYGQSPVPANLPPPITPTKVSAHPPATKDSPPPPPPPVRPKPSLSPVSTSNQYSGSTTSTPLHQPGGGSAGKEQTAGVKAAAAPPPPPPPPHASSASSSHKDTAQSSGVSPSVSSASSSGSPNQSPTSAKPTSTQRPAAQALGSLYIPPIHEVFANSKQSLLTQASPPWMSSRHNSLKEQPEWVHKDEQDSTTNTNATQSPEQSVEQEQSSKPPASSTSGSPRAPSVAKESVAATSKPVDSIVTAPTAVSARQAVPSQPLTPNSNRSAAPAADVSNPVQHQQTGTASGQQQQQQYGSQQQQQQQQTGRATGGLPAAGTVSSTVTMPVNRGYPQQAPVYAQAQPVYSTQSPAGTQKERIIPIQIEKSPVQAPVTAPTFAPPPYYSPSAQYGPASAQSSMQSPIAVGFATPHSGFTTPTAMFTNPNHFVNQGYNNISYPPTPTGQPHPHYQHHQHYAPPQTPPMHHPMHPQAQQQRMQQSTPTQQGQQVTNVRIVPIKVESGSDHTTVRGPLSNTPAIIQSGDRSYVIHDIPLEAKLRFLKNRLSDPRSSSNAQSWNGNNAPNQSRSFRVLQQITDTLDEAEAEKNAKEQSNNQSDQADGAKQQYSRSTAQQQDAAEGQLRRLQLSNEDKALMNRVKNQVDGEVYFHNEEDPRYRGAAIPSKAFRYLQNMTDSGQAANQSNNGSGNRAQQMFNKANYNDSDGDTQQSQQYVPPSEQKVEEPKKYTGGSIPSRSFKMLQAMTDAPGPGETDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8UXP3</accession>
    <name>A0A6E8UXP3_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Anoctamin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the anoctamin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8UXP3"/>
    <dbReference type="EnsemblMetazoa" id="ACON000095-RB">
        <property type="protein sequence ID" value="ACON000095-PB"/>
        <property type="gene ID" value="ACON000095"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010227"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000095"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039957"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032394">
        <property type="entry name" value="Anoct_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007632">
        <property type="entry name" value="Anoctamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049452">
        <property type="entry name" value="Anoctamin_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12308">
        <property type="entry name" value="ANOCTAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12308:SF83">
        <property type="entry name" value="ANOCTAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16178">
        <property type="entry name" value="Anoct_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04547">
        <property type="entry name" value="Anoctamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="286"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="362"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="442"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="486"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="535"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="661"/>
            <end position="683"/>
        </location>
    </feature>
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            <begin position="713"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="813"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="domain" description="Anoctamin dimerisation" evidence="11">
        <location>
            <begin position="55"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="Anoctamin transmembrane" evidence="10">
        <location>
            <begin position="275"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00009671"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU280814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF16178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000095-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="939" mass="108158" checksum="B8125EB093075FC5" modified="2020-08-12" version="1">MVNGNDQPAAVAATTTVPDSQPEAQTEPKPIVPPAGLAAAAAATTAPNRAQTWKYFQDQRRIVDYVLAFNGEDENAEAKQRRAIYQRNLESEGLQIETENCQRIHFVKIHVPENVLSHYCEIMKMQMPMKKLENQDKIIMRDFSIQSTLVRLFRRPLFNFVIIDRQKFAPPEYRLMYEYSRDKPYLFDDREQNFFTPSIRIAVAHFILERTYFSEAVEEKKDIGIRRLMEDQVYLDAYPLHDGCTDLRSSCQRALLLEEWASISKWIKHQPLDHIKEYFGVKIAMYFAWLGFYTHMLIPASVVGLICFFYGLLTYPANRISQEICDDNGTIMCPQCDKYCDYWYLRTTCNISKLAHIFDNEMTIVFSIFMSVWATLYLEMWKRYSSRIQHRWGITEYCSLAEPPRPQYLSRLKNIKKMMFNIATGAQEPSPPFWTKKFPSFLYSYSVIFLFILLTIAAVFGIVVYRMSLMTSKNIYGDGGSVSGKLIIFPATTAAINLLASTALTYAYQYVAEYMTNVEYRRTQTEYNESLNLKIYLFEFVNYYSSIFYIAFMKGKFPGYPAKYNRILHLRQEECSPGGCLMELCIQLAIIMVGKQAIGAITEILIPFLVQKFKEFRSVLGIDAGSAENGERLICCNQWTKDFNLINWHDRSLFNEYLKMVIQYGFITIFVVAFPLAPFFALLNNVFETRLDAKKFLLYYKRAVPQRVRDLGIWYNIMHVVGKVAVISSAFIIAFSSNFIPRLMYMNVVNPTGTDEGFVNHTLAYFNVSHFEANAAPENSTFSNITICRYSEYRNPPDDPRPYKRPSIYWQILAIRLTFVVIYQNLVSFVQIVVAWAIPDVPTKLQDQIKREQYLTNEYIIEQEKLKMALSGTGGCGGVGGSVAGTRYAQNGCVASEDSSPDDEKANYGSIADDNEYPLRMQTDDCEPYAAYGIVTSRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8UY08</accession>
    <name>A0A6E8UY08_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NF-kappa-B essential modulator NEMO CC2-LZ domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_040225297.2">
        <property type="nucleotide sequence ID" value="XM_040369363.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8UY08"/>
    <dbReference type="EnsemblMetazoa" id="ACON000283-RB">
        <property type="protein sequence ID" value="ACON000283-PB"/>
        <property type="gene ID" value="ACON000283"/>
    </dbReference>
    <dbReference type="GeneID" id="120950933"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000184"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000283"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_043319"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099518">
        <property type="term" value="P:vesicle cytoskeletal trafficking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038830">
        <property type="entry name" value="CCDC186"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18911:SF5">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 186"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18911">
        <property type="entry name" value="CTCL TUMOR ANTIGEN HD-CL-01"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="21"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="346"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000283-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="395" mass="45037" checksum="A4A9FAD9AC219685" modified="2020-08-12" version="1">MAETMATSTDDTNVESTRTQCDCSQQQLIEARAQLTALEQSNETLQKKLDGYRKDNENLVIKYAFVEKKVLDLKALVGDKDSQLKRHEQEVASLQKQIGTLKADKAKLQKNIESKLLQHVIAEETHQASEQVAEMERKVMELQAREIMLKHANDEQAGRIGTLQRANHELRDTIERQEKKHTSTLQTLKELETERDHLRQQMGQLQESFHQQQAAIAELQSKVEAASVAAQESSRMERAVAEQRAELEQLAGTLDEQRKDLEVLAARELELLALNKDLSEVNCLLQQEIATQESKTIAITLEYGKFVSMCQEYDAQIVSLANSLAAERQHRTEERLLMAKHIADRTRKLEQAESCLQQTLNELEANRKKYFALVKDFKREMKNAHCGVESSMVGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V1K7</accession>
    <name>A0A6E8V1K7_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">inositol-polyphosphate 5-phosphatase</fullName>
            <ecNumber evidence="1">3.1.3.56</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the inositol 1,4,5-trisphosphate 5-phosphatase type I family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.56" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8V1K7"/>
    <dbReference type="EnsemblMetazoa" id="ACON000203-RB">
        <property type="protein sequence ID" value="ACON000203-PB"/>
        <property type="gene ID" value="ACON000203"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002464"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000203"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040111"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004445">
        <property type="term" value="F:inositol-polyphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039737">
        <property type="entry name" value="INPP5A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000300">
        <property type="entry name" value="IPPc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12997:SF2">
        <property type="entry name" value="INOSITOL POLYPHOSPHATE-5-PHOSPHATASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12997">
        <property type="entry name" value="TYPE I INOSITOL-1,4,5-TRISPHOSPHATE 5-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22669">
        <property type="entry name" value="Exo_endo_phos2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00128">
        <property type="entry name" value="IPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inositol polyphosphate-related phosphatase" evidence="5">
        <location>
            <begin position="4"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="471"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="475"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000203-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="519" mass="58370" checksum="DD97026AC8FBCFBB" modified="2020-08-12" version="1">MENESLPILLITANVGSVFEDPSNLLHLWIKEFLDHIAARQPAFIALHLQEVGGKTYEKSMEYVQEFIKQLCESDELQGYNRIRVYLDEDYNSAEHFTALGNLYFVRSTVSHVLMWNFLTHEWDSVEGKSIHTGSIETVATKEKAKFPQQFFPECKWSRKGFLRTRWFLNGTVFDLVNIHLFHDASNLEACEEYPSVYCKSRRRALVHTLERFHKDTVNRPVPYFVFGDFNFRCDTEGVIKKLTEDLTMHRVQNTKNDSTKVQYRDATGTNVLTVGKKEFFHCDQSTFKEGWLRQFDRELESLRSILYEFPITFPPSYPYEEDPFQPVSYMATRCPAWCDRILVSPAARKLVWEHGLDDGKPAGDGAGSGANTSTVSYGIIGANVCMGDHKPVFLSFRIKTSQANGQLALGPDGKGGELTAQEFLDRLQEYTDLNTRIHDQQQEQLDKSNLTILHSQYVKITPINPDGGARQEVASPGTEGAAAAAAGGGGPPEDASACHGDAASSGTCCALIFRETTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V1K8</accession>
    <name>A0A6E8V1K8_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">GATA-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8V1K8"/>
    <dbReference type="EnsemblMetazoa" id="ACON002236-RB">
        <property type="protein sequence ID" value="ACON002236-PB"/>
        <property type="gene ID" value="ACON002236"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001830"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002236"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030518"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045165">
        <property type="term" value="P:cell fate commitment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00202">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000054">
        <property type="entry name" value="Transcription factor elt-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000032">
        <property type="entry name" value="Transcription factor GATA-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039355">
        <property type="entry name" value="Transcription_factor_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10071:SF281">
        <property type="entry name" value="BOX A-BINDING FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10071">
        <property type="entry name" value="TRANSCRIPTION FACTOR GATA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00320">
        <property type="entry name" value="GATA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00619">
        <property type="entry name" value="GATAZNFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00344">
        <property type="entry name" value="GATA_ZN_FINGER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GATA-type" evidence="11">
        <location>
            <begin position="497"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="domain" description="GATA-type" evidence="11">
        <location>
            <begin position="560"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="221"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="534"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="605"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="666"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="12"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="244"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="286"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="534"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="619"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="666"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="687"/>
            <end position="705"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002236-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="754" mass="81136" checksum="D4CEAEFDCAEE1E9E" modified="2020-08-12" version="1">MEATNEPKGEDQTPSSEVTSSVHQPVAHQPVSVSQASHTLTDVPVDGMIVNSELHTERSSPGQQTAVIINQHKQRMITTTGQIREITEGQEPPNESSDYDAVEYHHQAVQSAGGVEQHGYSYEPTHTTSQEHQQGVVVSSAATAVQASSVQVVKREMIEKEAALAAATAAANNNNNNPPETTIIPVQTQTIFVEYKNDGEEPVRYVNRYEDVKYHPHSRYIYQQPPGGLPLSSTLHSTGSGHPHVSHHPHHAHHHHHPHHGHGMVPGHQTHHHVHAQSAQVHQTIEAVSQHPSSVAVATQSGTTIQVQAQPHQQTIQVYETHEPGSGPGGEQVQQVAEGTVDAKTHYTNLEPVHTLASPQNYYIASDGYATGNYTTFLPQKETIYYHSPSPNPVLYKGDPTLTSSSIITKQIHYTPSLPGTQNMYENATGHSSSSPGAQQIYPGYWNGAGVDYNTNFTGTIVMDNAGGNVGEYATNGQHSWQISSLNDAYDPQLAAPPEHRECVNCGSSDTPLWRRDIVGHTLCNACALYTRQNPGTNRPPNRSQKAKQTVKTPPAQGNRRSGVTCANCQTTTTTLWRRNNQGDPVCNACGLYYKLHSVNRPLTMKKDGIQTRKRKPKSSQQIQPMNGLTTGKLLPSMIPSQYHSIAPEPKLLNAPQSHLVQTSQPMELHNSSPGQDPRYVDSSPGAPGGGGGSGSGSGGGGGGNSSPHLPPVQGNLAHHIPISVPVSMDSSRGANGEITSVITSTAVAERSGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V1M7</accession>
    <name>A0A6E8V1M7_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">choline-phosphate cytidylyltransferase</fullName>
            <ecNumber evidence="10">2.7.7.15</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">Ngousso</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="9">Phospholipid metabolism; phosphatidylcholine biosynthesis; phosphatidylcholine from phosphocholine: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytidylyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.15" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8V1M7"/>
    <dbReference type="EnsemblMetazoa" id="ACON002263-RE">
        <property type="protein sequence ID" value="ACON002263-PE"/>
        <property type="gene ID" value="ACON002263"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010575"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002263"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035988"/>
    <dbReference type="UniPathway" id="UPA00753">
        <property type="reaction ID" value="UER00739"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004105">
        <property type="term" value="F:choline-phosphate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031210">
        <property type="term" value="F:phosphatidylcholine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02174">
        <property type="entry name" value="CCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000016">
        <property type="entry name" value="Putative choline-phosphate cytidylyltransferase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041723">
        <property type="entry name" value="CCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045049">
        <property type="entry name" value="Pcy1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10739:SF13">
        <property type="entry name" value="CHOLINE-PHOSPHATE CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10739">
        <property type="entry name" value="CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="7" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cytidyltransferase-like" evidence="12">
        <location>
            <begin position="57"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="273"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="292"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="302"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="344"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="374"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="384"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00026101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002263-PE"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="406" mass="45621" checksum="80061D66B1E0217B" modified="2020-08-12" version="1">MSTCHTAAASHMATICKQAPYSTELDAIRERERCDYTQKITYQMARAGTAPRMIRVYADGIYDLFHQGHARQLMQAKNVFPNVYLIVGVCNDKLTHSRKGRTVMNDEERYEAVRHCRYVDEIVPDAPWELDDEFIEKHKIDFVAHDEIPYSTDDCNDVYAKIKAKGMFVATERTEGVSTSDIVARIVKDYDIYVRRNLARGYTAKELNVSFLSEKKFRFQNKMVELKDKVRKGKDDFITKWEERSTDLIKTFLLMFGKERISMFLSDSKQRIRSALSPPGSPGSGSNHSLNDCDDDDDLESPDSVLDSPPSKRSNVIRSRHHLLSEEDDEEQGLGGPRSPPPVLEDDEDDDDDDEAYLDSRSRPSSNYNISLIAGGGDGGSSGGSAALNSSSSGGSGSRTYGNRNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V3C8</accession>
    <name>A0A6E8V3C8_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Lipase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040225139.2">
        <property type="nucleotide sequence ID" value="XM_040369205.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8V3C8"/>
    <dbReference type="EnsemblMetazoa" id="ACON003082-RA">
        <property type="protein sequence ID" value="ACON003082-PA"/>
        <property type="gene ID" value="ACON003082"/>
    </dbReference>
    <dbReference type="GeneID" id="120950848"/>
    <dbReference type="KEGG" id="acoz:120950848"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005514"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003082"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032742"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000057">
        <property type="entry name" value="Lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006693">
        <property type="entry name" value="AB_hydrolase_lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025483">
        <property type="entry name" value="Lipase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11005">
        <property type="entry name" value="LYSOSOMAL ACID LIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04083">
        <property type="entry name" value="Abhydro_lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000862">
        <property type="entry name" value="Steryl_ester_lip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 7" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Partial AB-hydrolase lipase" evidence="9">
        <location>
            <begin position="69"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="8">
        <location>
            <position position="203"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="8">
        <location>
            <position position="377"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="8">
        <location>
            <position position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000862-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003082-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="432" mass="48742" checksum="E555DA943B30B860" modified="2020-08-12" version="1">MAVVRYGNAAAAQCIWPYRWSAVCSSWWPYILVLCTLTLPLRVVARSSDAFGGYPTVFAIDEEDGMLETSELIRKYGYPIEQHEITTADGYILTLTRIPPMRTKSDHSLPVLLVHGLFASSADFLIIGPNNSLAYLLADQGHDVWLADLRGNRYCRRHTHLSSDSREFWDYTWHEMGYYDLPATIDHVLSVTGARRLHYIGYSQGTTVFFVLASTRPEYNAKIARMYALSPAVYVQQVRSPIFRWLAENGPAVKCFLDAIGMWQVLPHNPVQYALQRALCPARIARPICVHLIEQMVGPNPNGTDRLAQHIMAGHNPSGASSKQLLHFAQLNRCGRFQQFAYERPDQNLAHYGREQPPAYNLSAVTAPVALFYALNDWMVGPANVVRLAAELPNVVSLTEVQDPHFNHLDFVAAKRVRALVYDSILEQLRDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V3Q0</accession>
    <name>A0A6E8V3Q0_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">MAP7 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MAP7 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8V3Q0"/>
    <dbReference type="EnsemblMetazoa" id="ACON001315-RC">
        <property type="protein sequence ID" value="ACON001315-PC"/>
        <property type="gene ID" value="ACON001315"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000686"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001315"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034867"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015630">
        <property type="term" value="C:microtubule cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051483">
        <property type="entry name" value="MAP7_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008604">
        <property type="entry name" value="MAP7_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15073:SF18">
        <property type="entry name" value="ENSCONSIN, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15073">
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        <property type="match status" value="1"/>
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        <property type="entry name" value="MAP7"/>
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        <location>
            <begin position="54"/>
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        </location>
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    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
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        </location>
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    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="217"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="341"/>
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        </location>
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    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="441"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="783"/>
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    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="812"/>
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    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="913"/>
            <end position="957"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="989"/>
            <end position="1041"/>
        </location>
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        <location>
            <begin position="57"/>
            <end position="70"/>
        </location>
    </feature>
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        <location>
            <begin position="71"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="107"/>
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        </location>
    </feature>
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        <location>
            <begin position="219"/>
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        <location>
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            <end position="375"/>
        </location>
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        <location>
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        <location>
            <begin position="395"/>
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            <begin position="1002"/>
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        <location>
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            <dbReference type="ARBA" id="ARBA00004245"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00007525"/>
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            <dbReference type="ARBA" id="ARBA00023212"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001315-PC"/>
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        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    <sequence length="1076" mass="118633" checksum="F67845C2D53C655E" modified="2020-08-12" version="1">MLTDESDRIPSTTLAVLRGILVKSLSRENLSLTDQQQLLGTRKARHVQFSLAGNRDATVQKQSRPSSANRELQKEREERIKQMKERQNEERQKKLEELKAQALAAQKFREQKEEERRRRMDDLRRRESDRRSQVEERRRAIIEADNERREYILRKNQERDQRMETKRRNDRGSIQFAFGSSTPRMIDTSDSGMCSSFWAHRRATSITNVAYTGAALTRRSSERELTDSASKKRATSASGLDRSTDDQRRMSSSMYEVFNWTSTSECPRKLTFSLAGSGINIDDPPTAAEYQPAASRNYASGKGKAILYACSCDSNGRTAMPSISPLPSFASLFKHDTAEMNYQRTVNRRKTDLMPTIPSPRDSSRSSLGTHTPRTPGSLRSGEMTPTSLNTSRPGSAMSSSTTASGIVYRRTLPAQRKPRPASIAVTGVSASGLKEGFATSKHRLSSAGSGSITTTPAKSASGTETPTKKPLTLHSAEKRSAGARGTTPKASSTPLQSPGPEKASRNLLDSLKKSADGPKKAPTPKTPAQKDDAVVVEAKQEPPSVAAPKVEELSAQQEAILQRDDGSQQASLVEVDQSFGQEEQPQQQQEEVVVPVQQEAPAPAQTEDAELPKQAEEAVTVEVVEQAPITKQQQQAPLEQQDVSAVQAAEPTVMVAEEVPENVNGSLTVEVSGAGADAMTASMIAKRINTEEEAKAALAERRRLAREEAERLAELERKRIEAEEQAERQRILEEEERLRKLEEETIRLAEEQRRMEEERLQQAIEEARRRDEEERKRREEEARQKAEREEAERKAREEAERQRVEMAERLKKEEKEREERRKRVEAIMSRTRAKGAANNTPTKNNEDDKENAMSKSQIVLSSTPAALPTGTAMSASTISMTDSFIASEIRQEQQQQQQQTQHQSLEQAMESLSLSNNNTTSSNSSSSSSDTLANSSTINNNINNNNNNTNGGNVQFDKSVTEKENLLLASSTAGAAVVVANNNLNNGSTSNGTVVEPATNEEGTNGTKGESLLLTTNGKSEQQSPAMMASSNGSSNATTANSTSDLIIEDALMGQTNGHKNGTMDNVLFPRVSDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V3Q6</accession>
    <name>A0A6E8V3Q6_ANOCL</name>
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        <submittedName>
            <fullName evidence="3">N-myc downstream regulated</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NDRG family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8V3Q6"/>
    <dbReference type="EnsemblMetazoa" id="ACON003238-RC">
        <property type="protein sequence ID" value="ACON003238-PC"/>
        <property type="gene ID" value="ACON003238"/>
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    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033801"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="entry name" value="alpha/beta hydrolase"/>
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        <property type="entry name" value="AB_hydrolase_fold"/>
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    <dbReference type="InterPro" id="IPR004142">
        <property type="entry name" value="NDRG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11034">
        <property type="entry name" value="N-MYC DOWNSTREAM REGULATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03096">
        <property type="entry name" value="Ndr"/>
        <property type="match status" value="2"/>
    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
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            <begin position="223"/>
            <end position="247"/>
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            <begin position="226"/>
            <end position="238"/>
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    <evidence type="ECO:0000256" key="1">
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            <dbReference type="ARBA" id="ARBA00005598"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003238-PC"/>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="272" mass="29762" checksum="10E72C81E8698978" modified="2020-08-12" version="1">MEKPKANDTASAIPVGSPSAKGVHRYAVNTEKSGEIIVTVQGDLSQQEKRAVFLTVHDLGTNHSSFEEFVNSACMIEIKERSCFIHIDVPGHADNAPNLADQLVGDNPDKEKIVSEFQSRLHSSLNSKNLKQYVKAFMSRKDLPLKNCKVDCLLITGIMSPYASVVEKLYKDLNKEKVTLLKVERAGDVLADAPAKVAQSILLFCKGQGLLTSVAMPGVDRNRAFSTSSSGSTEGSTGARRLSRGMSMEDYDKPNIRRLSVTINEQLPPIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V9P4</accession>
    <name>A0A6E8V9P4_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="19">3'-phosphoadenosine-5'-phosphosulfate synthase</fullName>
        </submittedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>sulfate + ATP + H(+) = adenosine 5'-phosphosulfate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:18133"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58243"/>
            <dbReference type="EC" id="2.7.7.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 1/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Sulfur metabolism; sulfate assimilation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the sulfate adenylyltransferase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">In the C-terminal section; belongs to the sulfate adenylyltransferase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the N-terminal section; belongs to the APS kinase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040226369.2">
        <property type="nucleotide sequence ID" value="XM_040370435.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8V9P4"/>
    <dbReference type="EnsemblMetazoa" id="ACON001256-RC">
        <property type="protein sequence ID" value="ACON001256-PC"/>
        <property type="gene ID" value="ACON001256"/>
    </dbReference>
    <dbReference type="GeneID" id="120951632"/>
    <dbReference type="CTD" id="40167"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_014208"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001256"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030554"/>
    <dbReference type="UniPathway" id="UPA00097"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004020">
        <property type="term" value="F:adenylylsulfate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004781">
        <property type="term" value="F:sulfate adenylyltransferase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050428">
        <property type="term" value="P:3'-phosphoadenosine 5'-phosphosulfate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000103">
        <property type="term" value="P:sulfate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd02027">
        <property type="entry name" value="APSK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00517">
        <property type="entry name" value="ATPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.400.10:FF:000002">
        <property type="entry name" value="ATP sulfurylase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000006">
        <property type="entry name" value="bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.400.10">
        <property type="entry name" value="Sulfate adenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00065">
        <property type="entry name" value="Adenylyl_sulf_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002891">
        <property type="entry name" value="APS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059117">
        <property type="entry name" value="APS_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025980">
        <property type="entry name" value="ATP-Sase_PUA-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024951">
        <property type="entry name" value="Sulfurylase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002650">
        <property type="entry name" value="Sulphate_adenylyltransferase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00455">
        <property type="entry name" value="apsK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003013">
        <property type="entry name" value="PRK03846.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00339">
        <property type="entry name" value="sopT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11055">
        <property type="entry name" value="BIFUNCTIONAL 3'-PHOSPHOADENOSINE 5'-PHOSPHOSULFATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11055:SF1">
        <property type="entry name" value="PAPS SYNTHETASE, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01583">
        <property type="entry name" value="APS_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01747">
        <property type="entry name" value="ATP-sulfurylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14306">
        <property type="entry name" value="PUA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="7" id="KW-0934">Plastid</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="13" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="APS kinase" evidence="16">
        <location>
            <begin position="45"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-sulfurylase PUA-like" evidence="18">
        <location>
            <begin position="229"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="Sulphate adenylyltransferase catalytic" evidence="17">
        <location>
            <begin position="394"/>
            <end position="620"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00037980"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF01747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF14306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001256-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="628" mass="70640" checksum="5F86019DB1B5AA10" modified="2020-08-12" version="1">MSGEPEKATKKQKTCLQVATNVTEQKHNVSREVRGQNLGLCRGFRGCTVWLTGLSGAGKTSIAFELEAYLVSKGIPAYGLDGDNIRTGLNKNLGFSQVDREENIRRVAEVAKLFADSGVVSICSFVSPFAEDREMARKIHKDADLKFFEIYIDTPLEVCETRDTKGLYKKARQGVIQGFTGVTQAYEAPDAPDLRVSTEGMTVRESTLKLIKLLESEAIIPKFLKEDEPLPELFVAESLKTALEAEAKTLPALALTEVELQWLQILAEGWAYPLKGFMREQEYLQALHFNCLLNEDETLRVNQSVPIVLSATEADKQRLEGVSALALQYGGRTVAVLRKPEFYYQRKEERCARQFGTSNRAHPYIKMIYESGDYLVGGEIEALERIQWNDGLDAYRLTPNELRQRFREIKADAVFAFQLRNPIHNGHALLMSDCRRQLLERGYKNPVLLLHPLGGWTKDDDVPLPVRMAQHQAVLDSGVLDRDHTVLAIFPSPMMYAGPTEVQWHAKARMNAGANHYIVGRDPAGMPHPDKDLYPDGNLYDGTHGARVLKMAPGLDSIEILPFRVAAYDKSCSQMAFFDPARKQDFDFISGTRMRTLARNGENPPNGFMEPKAWQILAEYYQSLQKSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VBA0</accession>
    <name>A0A6E8VBA0_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Ion transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="22">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 17">Membrane</location>
            <topology evidence="1 17">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15 17">Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family.</text>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON002578-RA">
        <property type="protein sequence ID" value="ACON002578-PA"/>
        <property type="gene ID" value="ACON002578"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002304"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002578"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042804"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005891">
        <property type="term" value="C:voltage-gated calcium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008331">
        <property type="term" value="F:high voltage-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098703">
        <property type="term" value="P:calcium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009582">
        <property type="term" value="P:detection of abiotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009581">
        <property type="term" value="P:detection of external stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050906">
        <property type="term" value="P:detection of stimulus involved in sensory perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042045">
        <property type="term" value="P:epithelial fluid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016322">
        <property type="term" value="P:neuron remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000007">
        <property type="entry name" value="Voltage-dependent L-type calcium channel subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000001">
        <property type="entry name" value="Voltage-dependent L-type calcium channel subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000043">
        <property type="entry name" value="Voltage-dependent L-type calcium channel subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000059">
        <property type="entry name" value="Voltage-dependent N-type calcium channel subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000011">
        <property type="entry name" value="Voltage-dependent N-type calcium channel subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2500">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050599">
        <property type="entry name" value="VDCC_alpha-1_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002077">
        <property type="entry name" value="VDCCAlpha1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45628">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL TYPE A SUBUNIT ALPHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45628:SF7">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL TYPE A SUBUNIT ALPHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00167">
        <property type="entry name" value="CACHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 16 17" id="KW-0106">Calcium</keyword>
    <keyword evidence="4 17" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="3 17" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="18" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0407">Ion channel</keyword>
    <keyword evidence="11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="9 17" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="42"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="80"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="113"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="171"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="259"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="292"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="452"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="482"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="577"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="653"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="788"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="827"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="857"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="924"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="1038"/>
            <end position="1063"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="1119"/>
            <end position="1137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="1149"/>
            <end position="1171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="1183"/>
            <end position="1204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="1277"/>
            <end position="1295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="1316"/>
            <end position="1333"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="21">
        <location>
            <begin position="40"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="21">
        <location>
            <begin position="451"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="21">
        <location>
            <begin position="789"/>
            <end position="1068"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="21">
        <location>
            <begin position="1176"/>
            <end position="1329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="384"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="715"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="18">
        <location>
            <begin position="677"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="397"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="719"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="748"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="273"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="631"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
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    <feature type="binding site" evidence="16">
        <location>
            <position position="1009"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00061395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR602077-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002578-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1335" mass="152852" checksum="4892DC61E9C5D86B" modified="2020-08-12" version="1">MGGPSSQGGQPLAGGGPTSLFILSEDNIIRKYTRFIIEWPPFEYAVLLTIIANCVVLALEEHLPHGDKTILAQKLEKTEAYFLGIFCVEASLKILALGFVMHKHSYLRNIWNIMDFFVVVTGSMTIFAEANVDVDLRMLRSFRVLRPLKLVSRIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGALHRSCYSLEDISQIVKEGEFPTPCNADNDTIAPTGAYVCNSSDSTCIEQWEGPNFGITSFDNIGFAMLTVFQCITMEGWTAILYWTNDALGSTFNWIYFVPLIVLGSFFMLNLVLGVLSGEFSNERGRVERQAQFHKCRSRQMFVEAMTSYLDWITQAEEVILAEERTTEEERLHIMEARRRAAAKRKKLKNLGKSKSTDTEEDDPDEDCGDDVFVPKRKKGYLKAKVKTQGKCKGFWRAEKRFRFWIRHTVKTQWFYWFVIVLVFFNTVCVAVEHYGQPNWLTQFLYYAEYVFLGLFMMEMWIKMYALGPRIYFESSFNRFDCVVISGSIFEVVWSEVKGGSFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLNSMRSIISLLFLLFLFILIFALLGMQLFGGQFNLPDGTPPTNFNTFPIALLTVFQILTGEDWNEVMYQGIESQGGHKKGMIYSLYFIILVLFGNYTLLNVFLAIAVDNLANAQELTAAEEEQLEENKEKQQMELDKEMEALHMQNDGSPPRVEVSSPSPTRGNGSGGSTKSKKKDEEKEEDDDEIPDGPKPMLPYSSMFVLSPTNPIRCGAHWVVNLRYFDFFIMVVISLSSIALAAEDPVEEDSPRNKILNFFDYAFTGVFTIEMLLKIVDLGVILHPGSYLREFWNVMDAVVVICAAVSFGFDMTGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVINILIVYILFQFIFAVIAVQLFNGKFFYCTDDSKHTSEECKGHFFVYDGADQLPRREPREWKTQSFHYDNVATAMLTLFAVQTGEGWPQVLQNSMAATYEDKGPIQNFRIEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQKSCIDFTIGARPLERYMPNKRNSFKYKVWRIVVSTPFEYFIMMLIVFNTLLLMMKYHNQGKEFEKSLKYLNMGFTGMFSVETILKIIGFGVKFHDAPTTLIDILSFMNLVFTFFFLLETIMKLIAYGCKNFFKDPWNIFDFITVIGSIIDALVLELGENSFNVGFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNMKFDPDTALNRHNNFQTFFGALLLLFRYLGILS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VBA5</accession>
    <name>A0A6E8VBA5_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ninjurin a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ninjurin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040221003.1">
        <property type="nucleotide sequence ID" value="XM_040365069.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VBA5"/>
    <dbReference type="EnsemblMetazoa" id="ACON002124-RD">
        <property type="protein sequence ID" value="ACON002124-PD"/>
        <property type="gene ID" value="ACON002124"/>
    </dbReference>
    <dbReference type="GeneID" id="120948573"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012474"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002124"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_036200"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042246">
        <property type="term" value="P:tissue regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007007">
        <property type="entry name" value="Ninjurin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12316:SF17">
        <property type="entry name" value="NINJURIN C, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12316">
        <property type="entry name" value="NINJURIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04923">
        <property type="entry name" value="Ninjurin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="64"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="108"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002124-PD"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="140" mass="15330" checksum="92431ABF5780F77D" modified="2020-08-12" version="1">MAMASILPASSGVGAESDRVLKSMDANRYATKKTIAQGMLDIALLTANASQLKYILQVGEKHEFYGLMLTLISISIILQVIMGVLNLSLSLMRDCRLHLPEYHRSANVINYIVTGAAVLVAMVNLMISAFDPKILKYTLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VBL2</accession>
    <name>A0A6E8VBL2_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Autophagy-related protein 16 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VBL2"/>
    <dbReference type="EnsemblMetazoa" id="ACON002315-RA">
        <property type="protein sequence ID" value="ACON002315-PA"/>
        <property type="gene ID" value="ACON002315"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001863"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002315"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040904"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034274">
        <property type="term" value="C:Atg12-Atg5-Atg16 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000421">
        <property type="term" value="C:autophagosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043495">
        <property type="term" value="F:protein-membrane adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000515">
        <property type="entry name" value="Autophagy-related 16, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045160">
        <property type="entry name" value="ATG16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19878">
        <property type="entry name" value="AUTOPHAGY PROTEIN 16-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19878:SF8">
        <property type="entry name" value="AUTOPHAGY-RELATED 16, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="13"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="71"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="150"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002315-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="216" mass="23658" checksum="66401453B26CA96B" modified="2020-08-12" version="1">MPTPRPNDSSHTLTGHSGKVLAAKFLGSAFLVTGSHDRTLKIWDLKNRSCTETKFAGSSCNDLVTTDSFSFISGHVDKKIRFWDVRTADCTANDIPLQGKITSLDLSKDGKFLLSCVRDDTINLLDLRQNRIVRTFRNDNFKVGCDWSRVAFSPSSSRIAAGSADGSVYIWNINGPLETVLKDPNGAAVTAVSWHPFSSTLASVDRAKKCTIWSNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VBL9</accession>
    <name>A0A6E8VBL9_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Na_H_Exchanger domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VBL9"/>
    <dbReference type="EnsemblMetazoa" id="ACON002324-RA">
        <property type="protein sequence ID" value="ACON002324-PA"/>
        <property type="gene ID" value="ACON002324"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006262"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002324"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037850"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051843">
        <property type="entry name" value="CPA1_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31102">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31102:SF21">
        <property type="entry name" value="NA[+]_H[+] HYDROGEN ANTIPORTER 2, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="30"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="149"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="218"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="7">
        <location>
            <begin position="3"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002324-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="260" mass="27858" checksum="7B28BAD7BB877F54" modified="2020-08-12" version="1">MTSCNDVLAIFLFGVILGVIFSTGDLTSQILQGPIGIVIGLVYGSLCGLALLYLPSYQAKYTNGLRFAMTALAGTLSVVGSKKVGYPSAGALGCIVTAFVAGTGWRKRPPTECNEVSMYLDLLWKFLKPVSFSLIGKEVKFSVLEGETVLYGFVTLLVAVLLRLIASYLSTVGSELNWKEKAYVTLAGFPKATVQAALGPAALDLARSMNATDEYGRAQTVLIVTVLAIILTAPLGALLMIKLAPRWLKRSTDERTDQYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VBM8</accession>
    <name>A0A6E8VBM8_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="13">G_PROTEIN_RECEP_F1_2 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VBM8"/>
    <dbReference type="EnsemblMetazoa" id="ACON002881-RA">
        <property type="protein sequence ID" value="ACON002881-PA"/>
        <property type="gene ID" value="ACON002881"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_013872"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002881"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035307"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14997">
        <property type="entry name" value="7tmA_ETH-R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24243">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24243:SF233">
        <property type="entry name" value="THYROTROPIN-RELEASING HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="234"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="274"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="314"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="356"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="387"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="441"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="487"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="253"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="525"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="717"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="17"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="540"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="717"/>
            <end position="726"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002881-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="760" mass="81905" checksum="25984FEC0EA7B343" modified="2020-08-12" version="1">MDRKRSPQRSRQSVTTASSSQPARPASRGGQGVGDPHHHHHHHHVLSLVDGHGWVVGGSQDHRQRHSTVAGQLSAVVRQAHCCVRQNAAPSLGGDSECVQFGAGERRSKTTPRERRSGTAGRRSAVATVVWIVLSQLIHVGSVAASQALDADPGNSNASNAGNVYGNRFGRLLLNGTRSVGGAGPSLLGASTTVLTTEPAPFDGGVAFAAAAAADGTASAGFNDSSEMPQIPEYIRATSMVFCIIIMCLGVIGNVMVPIVILKTKDMRNSTNIFLTNLSIADLLVLLVCTPTVLVEVNSPPEVWVLGEEMCNRAVPFVELTVAHASVLTILAISFERYYAICEPLKAGYVCTKTRALLICLAAWTVAAILTSRAAMCLTKASNGWTFTFFLMTISLFFVLPLAILVVLYAIIARNLIASDRSRLKIRLSKPELSHKARKQVVLMLGAVVLAFFTCLLPFRVLTLWIILVPEETFQQLAPERYYNLLYFSRIMLYLNSAVNPILYNLMSSKFRKGFLRLCSCSQWAGGAHGRRKGRNRSATFTTTTTTNTTSSSTYGGTGPSLALGSCRYDRSSNSAEDGPIGRNNGTQKLTLSLDDLRLQLPADGDCSTPPSLPAPSSFYRLNLVRQYSSPLLLFSSSPAPTIHRSTGASCHRGGAFKRQFNVTFDEPTGDGGGSLLASAEAEQLLLRIDQQQQQPEPRTQPLRNALRIKFPRFHGAAARTRSAAGGKRRSDSNGRTAATVKQLSLDETLLTAAMAGRKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VBN1</accession>
    <name>A0A6E8VBN1_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Lipid storage droplets surface-binding protein 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lipid droplet</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the perilipin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_049462980.1">
        <property type="nucleotide sequence ID" value="XM_049607023.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VBN1"/>
    <dbReference type="EnsemblMetazoa" id="ACON002890-RA">
        <property type="protein sequence ID" value="ACON002890-PA"/>
        <property type="gene ID" value="ACON002890"/>
    </dbReference>
    <dbReference type="GeneID" id="120951437"/>
    <dbReference type="CTD" id="42810"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010358"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002890"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_036335"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019915">
        <property type="term" value="P:lipid storage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010890">
        <property type="term" value="P:positive regulation of triglyceride storage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004279">
        <property type="entry name" value="Perilipin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14024:SF49">
        <property type="entry name" value="LIPID STORAGE DROPLETS SURFACE-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14024">
        <property type="entry name" value="PERILIPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03036">
        <property type="entry name" value="Perilipin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036881">
        <property type="entry name" value="PAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="378"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="386"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR036881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002890-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="401" mass="45316" checksum="F027148D93D35CD2" modified="2020-08-12" version="1">MVHRQLKRQHSGLPQMESLSRFSSIPVVETGIKTAGSVYERVKASNGLLTWGFETAESLTYALIDSLRPATKMIQGPLHQLDSIMCKSLDLVEQKVPSMYLPPEMMFWNTKEYMSDHLMKPVLSRANSMKNLGHAVLESRVSNYAAGRIDGALVVCDKYVERYLPTEPQDQPDSCGAAPPADDSNEMHVVKTFHRGQQLSRKLKRRLTFRTRQELSALKKQSTEAVHVVAYAAELIATNPREALQKAVELWRYLSKDEPENQARPRTLEQVAVLLTRESARKMVHLINFVTGAVTRVPKAVRAQTRELVHHFLFATERLMKTVHLEKAKAATLSEASGLVHRIQHTYDDLQNQTNLALERLAVFLSGRLEAEKITTSGNPRRRIQPRTNSNPMNSNINGVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VBN3</accession>
    <name>A0A6E8VBN3_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Chloride channel protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the chloride channel (TC 2.A.49) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VBN3"/>
    <dbReference type="EnsemblMetazoa" id="ACON002891-RD">
        <property type="protein sequence ID" value="ACON002891-PD"/>
        <property type="gene ID" value="ACON002891"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001039"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002891"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032829"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005247">
        <property type="term" value="F:voltage-gated chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03683">
        <property type="entry name" value="ClC_1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3080.10:FF:000003">
        <property type="entry name" value="Chloride channel 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.580.10:FF:000026">
        <property type="entry name" value="Chloride channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.580.10:FF:000032">
        <property type="entry name" value="Chloride channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3080.10">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014743">
        <property type="entry name" value="Cl-channel_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050970">
        <property type="entry name" value="Cl_channel_volt-gated"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001807">
        <property type="entry name" value="ClC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45720">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45720:SF10">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00654">
        <property type="entry name" value="Voltage_CLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00762">
        <property type="entry name" value="CLCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81340">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 13" id="KW-0129">CBS domain</keyword>
    <keyword evidence="11 14" id="KW-0868">Chloride</keyword>
    <keyword evidence="10" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 14" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 14" id="KW-0813">Transport</keyword>
    <keyword evidence="5" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="227"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="270"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="375"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="411"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="457"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="500"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="565"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="596"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="635"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="668"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="17">
        <location>
            <begin position="718"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="128"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1050"/>
            <end position="1087"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1117"/>
            <end position="1149"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="778"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="132"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="1050"/>
            <end position="1062"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="1073"/>
            <end position="1087"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="1119"/>
            <end position="1139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU361221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002891-PD"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1149" mass="128178" checksum="C5D7012F7CFAF85D" modified="2020-08-12" version="1">MWRKSGTDRAGKMRDRVGGHRGHPGSGGLERLRANSTDYTERIFHDEEFGEENVELVDEEWASFERVLRGQLWRINRGNVGSTFLFIARLRMENREFRERRLLNASMDNLPRQKKSSRKRRAQAFYPMPMPSMSDTVDQDSSSVSSSDEAYETDEDGYIRTLMYGRYTKDLGEYAKDEARRLRLLERRRKKDDKARNKELLDVRDNKFFKAASWLWRNTFARLGEDWVFLALLGIIMALLSYVMDKGISMCTNSRVWLYRDLTNHPVLQYLAWVSLPVCLILFSAGFVHLVAPQSIGSGIPEMKTILRGVALKEYLTFKTLVAKVIGLTATLGSGMPLGKEGPFVHIASIVSQLLSKIITSFQSIYENESRNTEMLAAACAVGVGACFAAPIGGVLFSIEVTTTYFAVRNYWRGFFAAVCGATVFRLLAVWFQKADTVTAMFSTNFTSDFPFDPQELFVFALIGLVCGLGGALYVWVHRKYVLFMRSNKKMNKFLQKNRFLYPGIVALIVATLSFPLGFGKYIAGELSTHDQVHQLFSNFTWTKHELTVEQAAVVSNWRTPETNVFANLIIYLLYTFFMSIIASTIPVPSGMFIPVFKIGAAFGRIIGEAMHLWFPHGVRYGGMLAPIIPGGYSVVGAAAFSGAVTHTVSVGVIVFEMTGQITHIVPVMIAALISNAVAALLQPSMYDSIILIKKLPYLPDLLPSGSGMYNIYVEDFMVRDVRYIWKGISYQQLKEILKANKSLRSLPIVDSPDNRTLLGSVQRYELIKMIDKHIGREKRLEVAAKLMKEAEEKAREEQERQKREAAEAAKARRPSRFEVVPAPDILKLRERANNEMLPPQARRESNASLTSGPVFGATQPKKSILKKTNSFTLKGFSPLSPHSPVHTPYTTITGTESRIRSAFDIIFRKSATLQDVTPDPEIGSIGTPSIVGSEVAPLTPGISKKVQLPRERVIDMSPEDQKAWEMEEMAKPIDVGNLHIDPAPFQLVERTSILKVHSLFSMVGINHAYVTNVGRLVGVVALKELRTAIESVNNGTLTPETVKAKQEEEARALAQKGKESDNNNGISAEDPLLPKRPGDTFSDKKVNNTITSMDSALSNSDNCSDIELDNINIDHGDTAATTNGTITTTTTTTPSTKHTNNHSGSQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VBN4</accession>
    <name>A0A6E8VBN4_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Troponin T</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the troponin T family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040222829.1">
        <property type="nucleotide sequence ID" value="XM_040366895.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VBN4"/>
    <dbReference type="EnsemblMetazoa" id="ACON002350-RC">
        <property type="protein sequence ID" value="ACON002350-PC"/>
        <property type="gene ID" value="ACON002350"/>
    </dbReference>
    <dbReference type="GeneID" id="120949532"/>
    <dbReference type="CTD" id="136032042"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011848"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002350"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041535"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005861">
        <property type="term" value="C:troponin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005523">
        <property type="term" value="F:tropomyosin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006936">
        <property type="term" value="P:muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006937">
        <property type="term" value="P:regulation of muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045214">
        <property type="term" value="P:sarcomere organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.350:FF:000003">
        <property type="entry name" value="Troponin T isoform 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027707">
        <property type="entry name" value="TNNT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001978">
        <property type="entry name" value="Troponin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038077">
        <property type="entry name" value="Troponin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11521">
        <property type="entry name" value="TROPONIN T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11521:SF1">
        <property type="entry name" value="TROPONIN T, SKELETAL MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00992">
        <property type="entry name" value="Troponin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90250">
        <property type="entry name" value="Troponin coil-coiled subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="52"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="215"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="301"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="15"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="52"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="73"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="125"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="152"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="215"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="307"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="319"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002350-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="374" mass="45123" checksum="C5D290DC94A16670" modified="2020-08-12" version="1">MSDDEEYSSEEEVVEETREEQGDDPEFIKRQDQKRSDLDEQLKEYINEWRKQRAKEEDELKKLKDKQAKRKVSRAEEEQRMAQRKKEEEERRVREVEEKKQREIDEKRKRLEEAEKKRQAMLQAMKDKDKKGPNFTITKKDSSFGMSNAQMERNKTKEQLEEEKKISLSFRIKPLEVDGLSADSLRARATELWETIVKLETEKYDLEERQKRQDYDLKELKERQKQQLRHKALKKGLDPEALTGKYPPKIQVASKYERRVDTRSYDDKKKLFEGGYSAYYQEKINKKWAQRQELFMARTKTKLPKWFGERPGKKSGDPETPEGEDEVKPDDEEVEEVEEVVEEVVEEEEEEEEEEEEEEEEEEEEEEEEEEEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VBN6</accession>
    <name>A0A6E8VBN6_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="14">P53 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">Ngousso</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the p53 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VBN6"/>
    <dbReference type="EnsemblMetazoa" id="ACON002352-RB">
        <property type="protein sequence ID" value="ACON002352-PB"/>
        <property type="gene ID" value="ACON002352"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010091"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002352"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041987"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08367">
        <property type="entry name" value="P53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.720">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012346">
        <property type="entry name" value="p53/RUNT-type_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011615">
        <property type="entry name" value="p53_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002117">
        <property type="entry name" value="p53_tumour_suppressor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11447">
        <property type="entry name" value="CELLULAR TUMOR ANTIGEN P53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11447:SF16">
        <property type="entry name" value="P53 PROTEIN LONG FORM VARIANT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00870">
        <property type="entry name" value="P53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00386">
        <property type="entry name" value="P53SUPPRESSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="p53 DNA-binding" evidence="13">
        <location>
            <begin position="130"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="317"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="279"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR602117-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002352-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="487" mass="54174" checksum="424C7ACDB6ECB6B8" modified="2020-08-12" version="1">MGEKLATSVIECDLCVPRCESDQVVIKSSFIRSYAMASNMEMLNGEIFGDINTALYQNGEDCQSLFRMNTNDLLPQQGSDLSELMLNDFFHNNGVAEMQCVKYETDAKLLTMLDGREEPTHYKKIPVLDDFTHPLLQFNVAISGKPCSASAWCYSNALEKLFVKKKTPVTFDVTYMQPSDYSRLKLRIMLVYSNSQYAYQTISRCQDDIAKDGAKDFAHKEHVVRCLNPDASFTGREKGVNFEDRLAVLVDLNNGGTPQHLEKQQTVPVSLEFLCQNSCPTMERRATTLVFTVENEHGTLLGRKSISVKICSCPKRDMEKDDSKATGGRENNKNKRKHANEVVPSNDQPPRKMTRQSSVDLKSTLNSNINATTSARESAAGPAAAATAAAATPLLGQIKREPSFTPLGYSLSNLSNSSNSTIDNDSSAVVLTLRLPDLACASEVAMYAFKHLSSILISCKDEKDKNRYAQYLSHCRRVRSKYASSHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VC07</accession>
    <name>A0A6E8VC07_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Ion transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="22">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 17">Membrane</location>
            <topology evidence="1 17">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15 17">Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family.</text>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON002578-RC">
        <property type="protein sequence ID" value="ACON002578-PC"/>
        <property type="gene ID" value="ACON002578"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002304"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002578"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042804"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005891">
        <property type="term" value="C:voltage-gated calcium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008331">
        <property type="term" value="F:high voltage-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098703">
        <property type="term" value="P:calcium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009582">
        <property type="term" value="P:detection of abiotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009581">
        <property type="term" value="P:detection of external stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050906">
        <property type="term" value="P:detection of stimulus involved in sensory perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042045">
        <property type="term" value="P:epithelial fluid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016322">
        <property type="term" value="P:neuron remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000007">
        <property type="entry name" value="Voltage-dependent L-type calcium channel subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000001">
        <property type="entry name" value="Voltage-dependent L-type calcium channel subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000043">
        <property type="entry name" value="Voltage-dependent L-type calcium channel subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000059">
        <property type="entry name" value="Voltage-dependent N-type calcium channel subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000011">
        <property type="entry name" value="Voltage-dependent N-type calcium channel subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2500">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050599">
        <property type="entry name" value="VDCC_alpha-1_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002077">
        <property type="entry name" value="VDCCAlpha1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45628">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL TYPE A SUBUNIT ALPHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45628:SF7">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL TYPE A SUBUNIT ALPHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00167">
        <property type="entry name" value="CACHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 16 17" id="KW-0106">Calcium</keyword>
    <keyword evidence="4 17" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="3 17" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="18" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0407">Ion channel</keyword>
    <keyword evidence="11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="9 17" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="42"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="80"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="113"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="171"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="259"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="292"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="452"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="482"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="577"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="653"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="788"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="827"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="857"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="924"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="1038"/>
            <end position="1063"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="1119"/>
            <end position="1137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="1149"/>
            <end position="1171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="1183"/>
            <end position="1204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="1277"/>
            <end position="1295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="1316"/>
            <end position="1333"/>
        </location>
    </feature>
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        <location>
            <begin position="40"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="21">
        <location>
            <begin position="451"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="21">
        <location>
            <begin position="789"/>
            <end position="1068"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="21">
        <location>
            <begin position="1176"/>
            <end position="1329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="385"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="715"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="18">
        <location>
            <begin position="677"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="719"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="748"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="273"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="631"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="1009"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00061395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR602077-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002578-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1335" mass="152982" checksum="E56848887FD5C9D8" modified="2020-08-12" version="1">MGGPSSQGGQPLAGGGPTSLFILSEDNIIRKYTRFIIEWPPFEYAVLLTIIANCVVLALEEHLPHGDKTILAQKLEKTEAYFLGIFCVEASLKILALGFVMHKHSYLRNIWNIMDFFVVVTGSMTIFAEANVDVDLRMLRSFRVLRPLKLVSRIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGALHRSCYSLEDISQIVKEGEFPTPCNADNDTIAPTGAYVCNSSDSTCIEQWEGPNFGITSFDNIGFAMLTVFQCITMEGWTAILYWTNDALGSTFNWIYFVPLIVLGSFFMLNLVLGVLSGEFAKEREKVENRQEFLKLRRQQQLEKELNGYVEWICKAEEVILAEERTTEEERLHIMEARRRAAAKRKKLKNLGKSKSTDTEEDDPDEDCGDDVFVPKRKKGYLKAKVKTQGKCKGFWRAEKRFRFWIRHTVKTQWFYWFVIVLVFFNTVCVAVEHYGQPNWLTQFLYYAEYVFLGLFMMEMWIKMYALGPRIYFESSFNRFDCVVISGSIFEVVWSEVKGGSFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLNSMRSIISLLFLLFLFILIFALLGMQLFGGQFNLPDGTPPTNFNTFPIALLTVFQILTGEDWNEVMYQGIESQGGHKKGMIYSLYFIILVLFGNYTLLNVFLAIAVDNLANAQELTAAEEEQLEENKEKQQMELDKEMEALHMQNDGSPPRVEVSSPSPTRGNGSGGSTKSKKKDEEKEEDDDEIPDGPKPMLPYSSMFVLSPTNPIRCGAHWVVNLRYFDFFIMVVISLSSIALAAEDPVEEDSPRNKILNFFDYAFTGVFTIEMLLKIVDLGVILHPGSYLREFWNVMDAVVVICAAVSFGFDMTGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVINILIVYILFQFIFAVIAVQLFNGKFFYCTDDSKHTSEECKGHFFVYDGADQLPRREPREWKTQSFHYDNVATAMLTLFAVQTGEGWPQVLQNSMAATYEDKGPIQNFRIEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQKSCIDFTIGARPLERYMPNKRNSFKYKVWRIVVSTPFEYFIMMLIVFNTLLLMMKYHNQGKEFEKSLKYLNMGFTGMFSVETILKIIGFGVKFHDAPTTLIDILSFMNLVFTFFFLLETIMKLIAYGCKNFFKDPWNIFDFITVIGSIIDALVLELGENSFNVGFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNMKFDPDTALNRHNNFQTFFGALLLLFRYLGILS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VC08</accession>
    <name>A0A6E8VC08_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Seipin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_040223510.2">
        <property type="nucleotide sequence ID" value="XM_040367576.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VC08"/>
    <dbReference type="EnsemblMetazoa" id="ACON003143-RB">
        <property type="protein sequence ID" value="ACON003143-PB"/>
        <property type="gene ID" value="ACON003143"/>
    </dbReference>
    <dbReference type="GeneID" id="120949932"/>
    <dbReference type="CTD" id="31245"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000132"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003143"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031789"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140042">
        <property type="term" value="P:lipid droplet formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23995">
        <property type="entry name" value="Seipin_BSCL2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009617">
        <property type="entry name" value="Seipin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21212">
        <property type="entry name" value="BERNARDINELLI-SEIP CONGENITAL LIPODYSTROPHY 2 HOMOLOG BSCL2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21212:SF0">
        <property type="entry name" value="SEIPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06775">
        <property type="entry name" value="Seipin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="50"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="172"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="249"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003143-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="325" mass="37426" checksum="D4976B193BCDEEF0" modified="2020-08-12" version="1">MGLLGLVLMILDPFKIVRNYVFKPVATLGLAAAEEYRARKEKSVTTTKNVFLKLLVVVLVGFSVVWASIFLYLYFYYSYMPSVLHVKDVHLNIRECQDNAYDCKPYPTANVALTNHQRFLMVGQPYKIILNLEMPESEHNGMFTVCGTVKDYGHVEVARSCRMSMLHYKSDLLKTILTFVFAPLLVFGYREEKQLVTVELFSNFLDDSTHPATNIDITIHCRDIQLYSAQLQVVANFTGLRYLMFNWPVLSALIGITTNLFFILIVCVLSWYHWDDTEWISDIRDRYQQIVKGVSKVNAAMEQTKAVNPLKDTDTPSKDWLLDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VE40</accession>
    <name>A0A6E8VE40_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">alpha-1,2-Mannosidase</fullName>
            <ecNumber evidence="18">3.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Involved in the maturation of Asn-linked oligosaccharides. Progressively trim alpha-1,2-linked mannose residues from Man(9)GlcNAc(2) to produce Man(5)GlcNAc(2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>N(4)-(alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 9A1,2,3B1,2,3) + 4 H2O = N(4)-(alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2) + 4 beta-D-mannose</text>
            <dbReference type="Rhea" id="RHEA:56008"/>
            <dbReference type="Rhea" id="RHEA-COMP:14356"/>
            <dbReference type="Rhea" id="RHEA-COMP:14367"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28563"/>
            <dbReference type="ChEBI" id="CHEBI:59087"/>
            <dbReference type="ChEBI" id="CHEBI:139493"/>
            <dbReference type="EC" id="3.2.1.113"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>N(4)-(alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 8A1,2,3B1,3) + 3 H2O = N(4)-(alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2) + 3 beta-D-mannose</text>
            <dbReference type="Rhea" id="RHEA:56028"/>
            <dbReference type="Rhea" id="RHEA-COMP:14358"/>
            <dbReference type="Rhea" id="RHEA-COMP:14367"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28563"/>
            <dbReference type="ChEBI" id="CHEBI:59087"/>
            <dbReference type="ChEBI" id="CHEBI:60628"/>
            <dbReference type="EC" id="3.2.1.113"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 16">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Endomembrane system</location>
            <topology evidence="14">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 18">Belongs to the glycosyl hydrolase 47 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.-" evidence="18"/>
    <dbReference type="RefSeq" id="XP_049466171.1">
        <property type="nucleotide sequence ID" value="XM_049610214.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VE40"/>
    <dbReference type="EnsemblMetazoa" id="ACON000558-RB">
        <property type="protein sequence ID" value="ACON000558-PB"/>
        <property type="gene ID" value="ACON000558"/>
    </dbReference>
    <dbReference type="GeneID" id="120949019"/>
    <dbReference type="CTD" id="31957"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007412"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000558"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037460"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004571">
        <property type="term" value="F:mannosyl-oligosaccharide 1,2-alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000002">
        <property type="entry name" value="alpha-1,2-Mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001382">
        <property type="entry name" value="Glyco_hydro_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050749">
        <property type="entry name" value="Glycosyl_Hydrolase_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036026">
        <property type="entry name" value="Seven-hairpin_glycosidases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11742:SF6">
        <property type="entry name" value="MANNOSYL-OLIGOSACCHARIDE ALPHA-1,2-MANNOSIDASE IA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11742">
        <property type="entry name" value="MANNOSYL-OLIGOSACCHARIDE ALPHA-1,2-MANNOSIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01532">
        <property type="entry name" value="Glyco_hydro_47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00747">
        <property type="entry name" value="GLYHDRLASE47"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48225">
        <property type="entry name" value="Seven-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 16" id="KW-0106">Calcium</keyword>
    <keyword evidence="9 17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10 18" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 18" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="20" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026034205" description="alpha-1,2-Mannosidase" evidence="20">
        <location>
            <begin position="20"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="56"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="15">
        <location>
            <position position="232"/>
        </location>
    </feature>
    <feature type="active site" evidence="15">
        <location>
            <position position="365"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="15">
        <location>
            <position position="474"/>
        </location>
    </feature>
    <feature type="active site" evidence="15">
        <location>
            <position position="499"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="585"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="428"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00054774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00060399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU361193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000558-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="608" mass="69436" checksum="2B4109299AAAC1D3" modified="2020-08-12" version="1">MSVVATAMFLVAMTGVTLVVRQQRDLADPLQPAVPVPHHRYQERFLSYDDHVRFGGLGGSSPPDLDPNGQQQQQQQHYYHYTHSKRQAALEEGPAPSAADIEEARIRRSLYDSSSYVPRYINATGWLRIEEGAANPVGSSIDHEKRDYVRQMMVHAWSNYKLYAWGKNELRPLSKRGHSNSIFGSFDLGATIVDGLDTLYLMGLHKEFDEGREWVERKFTLDNVDAELSVFETNIRFIGGFLTCYAFTGDRMFLEKARYVADKLLPAFQTPTGIPYALVNVHNGVSKNYGWASGGSSILSEFGTLHMEFAYLSDITGDSVYRERVQTIRAVLKDIEKPKGLYPNYLNPKTGKWGQQHMSLGALGDSFYEYLLKAWIQSGQEDWEAREMYDDAMQAIIKHMVRNTPSGLTYVSDMKFDRLEHKMDHLACFAGGLFGLGGTSLNNQYSQQYMEIGEGLTNTCHESYIRTYTRLGPESFRFNDGVEAKALKAQEKYYILRPETFESYFIMWRLTHDQKYRDWGWDAVQALEKHCRTPTGYTGLKNVYLTEPVKDDVQQSFFLAETLKYLYLLFSDDSLLPLDDWVFNTEAHPLPVKDRNPLYRPAAGSSSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VE41</accession>
    <name>A0A6E8VE41_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Moesin/ezrin/radixin homolog 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Involved in connections of major cytoskeletal structures to the plasma membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Interacts with cytoskeletal actin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell junction</location>
            <location evidence="4">Adherens junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Microvillus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Cell projection</location>
            <location evidence="14">Rhabdomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VE41"/>
    <dbReference type="EnsemblMetazoa" id="ACON000562-RB">
        <property type="protein sequence ID" value="ACON000562-PB"/>
        <property type="gene ID" value="ACON000562"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006774"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000562"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041662"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098592">
        <property type="term" value="C:cytoplasmic side of apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005902">
        <property type="term" value="C:microvillus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016028">
        <property type="term" value="C:rhabdomere"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048646">
        <property type="term" value="P:anatomical structure formation involved in morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042051">
        <property type="term" value="P:compound eye photoreceptor development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010669">
        <property type="term" value="P:epithelial structure maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007315">
        <property type="term" value="P:pole plasm assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042127">
        <property type="term" value="P:regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050793">
        <property type="term" value="P:regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006403">
        <property type="term" value="P:RNA localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13194">
        <property type="entry name" value="FERM_C_ERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17187">
        <property type="entry name" value="FERM_F1_ERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000003">
        <property type="entry name" value="Radixin isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.10:FF:000002">
        <property type="entry name" value="radixin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000013">
        <property type="entry name" value="radixin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.450:FF:000001">
        <property type="entry name" value="radixin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.360.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011174">
        <property type="entry name" value="ERM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011259">
        <property type="entry name" value="ERM_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041789">
        <property type="entry name" value="ERM_FERM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046810">
        <property type="entry name" value="ERM_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000798">
        <property type="entry name" value="Ez/rad/moesin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019747">
        <property type="entry name" value="FERM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018979">
        <property type="entry name" value="FERM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018980">
        <property type="entry name" value="FERM_PH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008954">
        <property type="entry name" value="Moesin_tail_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23281">
        <property type="entry name" value="MERLIN/MOESIN/EZRIN/RADIXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00769">
        <property type="entry name" value="ERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20492">
        <property type="entry name" value="ERM_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09380">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00373">
        <property type="entry name" value="FERM_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09379">
        <property type="entry name" value="FERM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002305">
        <property type="entry name" value="ERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00935">
        <property type="entry name" value="BAND41"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00661">
        <property type="entry name" value="ERMFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01196">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48678">
        <property type="entry name" value="Moesin tail domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00660">
        <property type="entry name" value="FERM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00661">
        <property type="entry name" value="FERM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="9" id="KW-0965">Cell junction</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="18" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FERM" evidence="20">
        <location>
            <begin position="8"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="460"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="18">
        <location>
            <begin position="305"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="471"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="489"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <begin position="63"/>
            <end position="66"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol)</name>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="281"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol)</name>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00043944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00057575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00063162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR002305-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000562-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="575" mass="68012" checksum="EC79FE532674F137" modified="2020-08-12" version="1">MVASSKMMNVRVTTMDAELEFAIQQGTTGKQLFDQVVKTIGLREVWFFGLQYTDSKGDNTWIKLYKKVMSQDVQKGDPLLFKFRAKFYPEDVAEELIQDITLRLFYLQVKNAILSDEIYCPPETSVLLASYAVQARHGDYNKGTHVPGFLAKDRLLPQRVIDQHKMSKDEWENSITTWWQEHRGLLREDAMMEYLKIAQDLEMYGVNYFEIRNKKGTELWLGVDALGLNIYEKEDRLTPKIGFPWSEIRNISFNDRKFIIKPIDKKAPDFVFFAPRVRINKRILALCMGNHELYMRRRKPDTIDVQQMKAQARDEKNAKQQEREKLQLALAARERAEKKQQEYEDRLRSMQEEMERKQANLSEAQDTIRRLQEQLNQVQAAKEELEQRQNELHEMMQRLEETKNMEATERAKLEEEIRVKQLEMQKIQEEVTLKDSETKRLHEEVEEAIRKQAAAALQAATTTPKHHHVEEEEENEEELINGENGTQDFSKDFDTDEHIKDPVEERRTLAERNERLQDQLKALKQDLALSRDDTMETPNDKIHRENVRQGRDKYKTLREIRKGNTKRRVDQFENM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VE44</accession>
    <name>A0A6E8VE44_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S1 family. CLIP subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VE44"/>
    <dbReference type="EnsemblMetazoa" id="ACON000573-RA">
        <property type="protein sequence ID" value="ACON000573-PA"/>
        <property type="gene ID" value="ACON000573"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004189"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000573"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_029288"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000068">
        <property type="entry name" value="transmembrane protease serine 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051333">
        <property type="entry name" value="CLIP_Serine_Protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24260:SF147">
        <property type="entry name" value="EG:BACR7A4.3 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0720">Serine protease</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026228046" description="Peptidase S1 domain-containing protein" evidence="4">
        <location>
            <begin position="23"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="5">
        <location>
            <begin position="120"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000573-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="362" mass="39467" checksum="495EC1527FB63983" modified="2020-08-12" version="1">MMNRPRIAAIMLPLFCLSVVVAQKGSSSTDTVFMDSVLYEGDKCGLPGMRTGTCQKAADCPQGIRANGARCEFSGNDPVVCCPTEAPGTGDGTSNRFTARIAKQECERFTSASANIIDHISGRAIEALRGEFPFVALVNFRGEEGEEVKLTRCGASLIAPRFLLTAAHCLKDLNPVTVEIGFIQLSDTEKDEYEIKQVHLHEGHKSRRNDIALIELKNNVTYKQDVGPICLNTDRPEIGPSINLTVMGWGADGDGQRADKLMKGTVYEIPLDECVQRFRDAKQRISLGEDQLCALGEKVNDETTDACQGDSGGPLVMTVRHKFYLVGVVSTGAVCGGSLPGIYTRVSRYLEWIEQRVWGSPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VE45</accession>
    <name>A0A6E8VE45_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">AMP deaminase</fullName>
            <ecNumber evidence="9">3.5.4.6</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">AMP deaminase plays a critical role in energy metabolism. Catalyzes the deamination of AMP to IMP and plays an important role in the purine nucleotide cycle.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>AMP + H2O + H(+) = IMP + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:14777"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="3.5.4.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; IMP biosynthesis via salvage pathway; IMP from AMP: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the metallo-dependent hydrolases superfamily. Adenosine and AMP deaminases family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.6" evidence="9"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8VE45"/>
    <dbReference type="EnsemblMetazoa" id="ACON000577-RA">
        <property type="protein sequence ID" value="ACON000577-PA"/>
        <property type="gene ID" value="ACON000577"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_003237"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000577"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040059"/>
    <dbReference type="UniPathway" id="UPA00591">
        <property type="reaction ID" value="UER00663"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003876">
        <property type="term" value="F:AMP deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046033">
        <property type="term" value="P:AMP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032264">
        <property type="term" value="P:IMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01319">
        <property type="entry name" value="AMPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.800.20:FF:000001">
        <property type="entry name" value="AMP deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000035">
        <property type="entry name" value="Probable amp deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.800.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006650">
        <property type="entry name" value="A/AMP_deam_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006329">
        <property type="entry name" value="AMPD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01429">
        <property type="entry name" value="AMP_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11359">
        <property type="entry name" value="AMP DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11359:SF0">
        <property type="entry name" value="AMP DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19326">
        <property type="entry name" value="AMP_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001251">
        <property type="entry name" value="AMP_deaminase_met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00485">
        <property type="entry name" value="A_DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00054146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR001251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000577-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="666" mass="77097" checksum="D83CE3E7CD59C3DD" modified="2020-08-12" version="1">MEQDRHSIAPEVHPDEQLPFDEHDFVAHFQRVSISGEDTSGVPLDDLERASTLLVKALELREKYMRNSHQAFPQTTARFLKSTHSSTITHQERKSIADHPVNPPQSTQSPWVVEIPEDLNYSIRPVHGVFEVFPTVESTEPLPYQYTRLPDFVQDMQMMCSMIADGPLKSFCYRRLSYLYSKFQLHVLLNELRELASQKAVPHRDFYNIRKVDTHIHAASCMNQKHLLRFIKKTLKNSADEVVTVTKGTPMTLAQVFQSMNLTTYDLTVDMLDVHADRNTFHRFDKFNAKYNPIGESRLREVFLKTDNYLNGKYFANIIKEVASDFEESKYQNAELRLSIYGKSPDEWYKLAKWAIDGNVYSDNIRWLIQIPRLYDIFKTNQLMSSFQQILDNVFKPLFEATNNPSKHPEIHTFLQYVIGFDSVDDESKPENPLFDGDVTPPADWTDVENPPYAYYIYYMYANMTVLNHFRAARGMNTFVLRPHCGEAGPVQHLVCGYLMAENISHGLLLRKVPVLQYLYYLAQIGIAMSPLSNNSLFLNYDRNPFPEYLARGLVVSLSTDDPLQFHYTKEPLMEEYSIAAQVWKLSSCDMCELARNSVLMSGFPHKMKQHWLGPNYTREGVAGNDITRTNVPDIRVAFRYESLLDELANIFKVNNEQKMQYASQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VE48</accession>
    <name>A0A6E8VE48_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="17">EF-hand domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">Ngousso</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; phospholipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="13">Phospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040230217.2">
        <property type="nucleotide sequence ID" value="XM_040374283.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VE48"/>
    <dbReference type="EnsemblMetazoa" id="ACON000596-RA">
        <property type="protein sequence ID" value="ACON000596-PA"/>
        <property type="gene ID" value="ACON000596"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON000596-RC">
        <property type="protein sequence ID" value="ACON000596-PC"/>
        <property type="gene ID" value="ACON000596"/>
    </dbReference>
    <dbReference type="GeneID" id="120953796"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000368"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000596"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030218"/>
    <dbReference type="UniPathway" id="UPA00085"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042171">
        <property type="term" value="F:lysophosphatidic acid acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008374">
        <property type="term" value="F:O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07991">
        <property type="entry name" value="LPLAT_LPCAT1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045252">
        <property type="entry name" value="LPCAT1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23063:SF52">
        <property type="entry name" value="LYSOPHOSPHATIDYLCHOLINE ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23063">
        <property type="entry name" value="PHOSPHOLIPID ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="81"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="16">
        <location>
            <begin position="472"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00025707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000596-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="522" mass="58977" checksum="5D4E3BA0E94D3224" modified="2020-08-12" version="1">MAATAEWEPPVAPEAAPVAQPERKGTAPAPGPPAAINRKAENNQPDEIHYVNPFVHKLVWDDPIDKAKTALLTVILLPFRVVLILVCLVVAWALANIGLYGLSKEDLRTKPLVGWRRRLKGPLGEFATLAYTCAGLGITIRGRQASRAEAPVLVVSPHSSFLDAVIIYVTGLSSPLVRNADRNLGKLIDYAQPIYVCREDPHSRQTTIREIIQRANSKEDWPQILIFPEGTCTNRTSLIKFKPGAFYPGVPIQPVLMRYPNKVDTVTWTWEGPNAIQLLWRTLTQFHTFCEIEFLPVYYPSEAEKANPKLYADNVRMLMAKALDIPISDYTFDDCKLMTFVKNVGMPHPAAIADIGKLRETLKIAATDRESKIVASERQFTDANSLLTYPQFAERMQLDVQQEAAHNLFHKLIRPETPDVIDFREYLLLALFLITLYKPKLIFVEYLFHLYGVNGRVNRTQLYQTLQNLVRISQKELNSIFLQADPDNLGTIDYSQFVKAIERNPLLAKLQKNNDPNNLRLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VFI8</accession>
    <name>A0A6E8VFI8_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="21">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="17">Postsynaptic cell membrane</location>
            <topology evidence="17">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VFI8"/>
    <dbReference type="EnsemblMetazoa" id="ACON002971-RA">
        <property type="protein sequence ID" value="ACON002971-PA"/>
        <property type="gene ID" value="ACON002971"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_008704"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002971"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037004"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022848">
        <property type="term" value="F:acetylcholine-gated monoatomic cation-selective channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007271">
        <property type="term" value="P:synaptic transmission, cholinergic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19031">
        <property type="entry name" value="LGIC_ECD_nAChR_proto_alpha-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19064">
        <property type="entry name" value="LGIC_TM_nAChR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000013">
        <property type="entry name" value="Acetylcholine receptor subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000012">
        <property type="entry name" value="Acetylcholine receptor subunit alpha-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002394">
        <property type="entry name" value="Nicotinic_acetylcholine_rcpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00860">
        <property type="entry name" value="LIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00254">
        <property type="entry name" value="NICOTINICR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16 18" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9 18" id="KW-0406">Ion transport</keyword>
    <keyword evidence="15" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="10 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 18" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0770">Synapse</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 18" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026372502" evidence="18">
        <location>
            <begin position="20"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="246"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="277"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="311"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="19">
        <location>
            <begin position="31"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="20">
        <location>
            <begin position="252"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002971-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="425" mass="48703" checksum="94CB2C8583B40375" modified="2020-08-12" version="1">MLNAKVILLCLVASSSAQGRSSHGHANPDAKRLYDDLLSNYNRLIRPVVNNTETLTVWLGLKLSQLIEVSLRNQVMTTNLWVKQKWFDYKLRWDPEEYGGVEMLYVPSEQIWLPDIVLYNNWDGNYEVTLMTKATLKYTGEVFWEPPAIYKSSCEMNVEYFPYDEQTCLMKFGSWTYNGAQVELRHLDQIPGSNLVQIGIDLSEFYLSVEWDILEVPASRNEEYYPCCPEPFSDITFKLTMRRKTLFYTVNLIIPCVGITFLTVLVFYLPSDSGEKVTLCISILVSLTVFFLLLAEIIPPTSLAVPLLGKYLLFTMILVSLSVWTTVCVLNVWYRSTSTHKMSPFVRRLFLEIMPKILMMRRAKYTLPDYDDSTPSNGYTNEIEMSVSDFPGEFKEGGDSFDNIGVNLSHGSGKSQCLGMRPPHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VFU4</accession>
    <name>A0A6E8VFU4_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mitochondrial proton/calcium exchanger protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Leucine zipper-EF-hand-containing transmembrane protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="24">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LETM1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VFU4"/>
    <dbReference type="EnsemblMetazoa" id="ACON003296-RA">
        <property type="protein sequence ID" value="ACON003296-PA"/>
        <property type="gene ID" value="ACON003296"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005203"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003296"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030175"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030003">
        <property type="term" value="P:intracellular monoatomic cation homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033122">
        <property type="entry name" value="LETM1-like_RBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044202">
        <property type="entry name" value="LETM1/MDM38-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059005">
        <property type="entry name" value="LETM1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14009">
        <property type="entry name" value="LEUCINE ZIPPER-EF-HAND CONTAINING TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14009:SF1">
        <property type="entry name" value="MITOCHONDRIAL PROTON_CALCIUM EXCHANGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26561">
        <property type="entry name" value="LETM1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07766">
        <property type="entry name" value="LETM1_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51758">
        <property type="entry name" value="LETM1_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0050">Antiport</keyword>
    <keyword evidence="10" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="13 19" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="14" id="KW-0406">Ion transport</keyword>
    <keyword evidence="16 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15 18" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="7 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="254"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="Letm1 RBD" evidence="23">
        <location>
            <begin position="300"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="22">
        <location>
            <begin position="730"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="124"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="524"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="798"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="19">
        <location>
            <begin position="607"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="128"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="524"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="798"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="839"/>
            <end position="848"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00031360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003296-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="880" mass="97666" checksum="489DB50E8ADA2814" modified="2020-08-12" version="1">MSVLLRNQSQLFGMNRCYRNVHPNVRVRFVSYRTKRPIGLISAQYDSLLQPHTIFSGSTGSRTWTPAGGVRQDGGVPLLDALGYRCGTVLPGSPSIRCISTTSWLGDQPSSKVEVTVQTLKKKEKEQQQQQQQQQQLANGVPNGAAAAPTAATSKVLADLGTSAANAPVGASTGASADAQSQTAPPAAAVVAAAPVVKKTLKQRIWAELVHYYHGFRLLFIDINISRKLLWRVLNGKTLTRREHKLLIRTTSDLFRLVPFSVFIIVPFMELLLPLAIKLFPGMLPSTFQTATEREDKIKQNLKVKIEMAKFLQKTLDDMAVQNKEHRSQAAKDFSEFFTRIRTTENFTISNEEILKFSKLFEDEITLDSLNRQQLQALCRVLEVSPIGTSNLLRFQLRLKLRNLAADDRTIQKEGIASLNLSELQAACRARGMRAYGASEERLKSQLQEWINLSLNEKVPPSLLLLSRALVLPENITTGDKLKATISSLPDSVATVTKAAIGEREGKIDNKTKIEVIKEEERKIKEEREEEKEKQKEQQELVDGAPVLTADGKTIEPGTVVPEQPEIIFAPAPATTIVLDKIPPQPHTVEEISSKDLEVLGDALGTLSKDKKSLLVEKEEIRDLKEEIADYQEDVQELQEVVTAASNQEEVQVKESRAAKLLFKKVNSMINRMDTVLTELERKEKQLKEKVHAAEQSEEVQKPPAADEELVRIDELMSAIKKIQNVTDDSRLDQISKILGKIDDDQDGQIKVEDVLKVIETIGKENVKLNAKQVDELIDLLDKEEELEAEDKIEKALTKSLEAKEKQKEQKEKEKEKLLELTDKATDLSSLAPPPSPTDPTKQVSSDAMPQLPPSPSSSTSSSPPPPPPAPSSSTGVPPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VFV5</accession>
    <name>A0A6E8VFV5_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Odorant-binding protein 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PBP/GOBP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040220018.2">
        <property type="nucleotide sequence ID" value="XM_040364084.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VFV5"/>
    <dbReference type="EnsemblMetazoa" id="ACON003307-RB">
        <property type="protein sequence ID" value="ACON003307-PB"/>
        <property type="gene ID" value="ACON003307"/>
    </dbReference>
    <dbReference type="GeneID" id="120948096"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006457"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003307"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040287"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007608">
        <property type="term" value="P:sensory perception of smell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23992">
        <property type="entry name" value="PBP_GOBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.20:FF:000001">
        <property type="entry name" value="General odorant-binding protein lush"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.20">
        <property type="entry name" value="Pheromone/general odorant binding protein domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006170">
        <property type="entry name" value="PBP/GOBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036728">
        <property type="entry name" value="PBP_GOBP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11857:SF45">
        <property type="entry name" value="GENERAL ODORANT-BINDING PROTEIN 83A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11857">
        <property type="entry name" value="ODORANT BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01395">
        <property type="entry name" value="PBP_GOBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00485">
        <property type="entry name" value="MEALWORMBTLB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00708">
        <property type="entry name" value="PhBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47565">
        <property type="entry name" value="Insect pheromone/odorant-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026344321" description="Odorant-binding protein 2" evidence="5">
        <location>
            <begin position="19"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003307-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="141" mass="16047" checksum="8321EB262DB1D6DD" modified="2020-08-12" version="1">MLTIVVATSICLMATVSANVPNSLSPELLQQMGQFRSECLRETGTTDEQIEQFNSPQSVQASHELQCYMYCMFRLHNVTRPNGELDLIDVYHAIPKQFNSIALKVLAKCNKSTGPIADACERAYSHHRCWKETEPEHYHLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VG62</accession>
    <name>A0A6E8VG62_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">LIM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_049461697.1">
        <property type="nucleotide sequence ID" value="XM_049605740.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VG62"/>
    <dbReference type="EnsemblMetazoa" id="ACON003429-RA">
        <property type="protein sequence ID" value="ACON003429-PA"/>
        <property type="gene ID" value="ACON003429"/>
    </dbReference>
    <dbReference type="GeneID" id="120948042"/>
    <dbReference type="CTD" id="40999"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010066"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003429"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033877"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005911">
        <property type="term" value="C:cell-cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045216">
        <property type="term" value="P:cell-cell junction organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001046">
        <property type="term" value="P:positive regulation of integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900026">
        <property type="term" value="P:positive regulation of substrate adhesion-dependent cell spreading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09331">
        <property type="entry name" value="LIM1_PINCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09332">
        <property type="entry name" value="LIM2_PINCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09333">
        <property type="entry name" value="LIM3_PINCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09334">
        <property type="entry name" value="LIM4_PINCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09335">
        <property type="entry name" value="LIM5_PINCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000011">
        <property type="entry name" value="Lim and senescent cell antigen-like-containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000017">
        <property type="entry name" value="Lim and senescent cell antigen-like-containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000021">
        <property type="entry name" value="Lim and senescent cell antigen-like-containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000092">
        <property type="entry name" value="LIM domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000094">
        <property type="entry name" value="LIM domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047944">
        <property type="entry name" value="LIMS1/2-like_LIM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017351">
        <property type="entry name" value="PINCH-1-4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047946">
        <property type="entry name" value="PINCH-1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24210">
        <property type="entry name" value="LIM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24210:SF0">
        <property type="entry name" value="LIM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="5"/>
    </dbReference>
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        <property type="entry name" value="PINCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0965">Cell junction</keyword>
    <keyword evidence="6 7 8" id="KW-0440">LIM domain</keyword>
    <keyword evidence="2 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="9">
        <location>
            <begin position="6"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="9">
        <location>
            <begin position="69"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="9">
        <location>
            <begin position="199"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="9">
        <location>
            <begin position="259"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR038003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003429-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="336" mass="38954" checksum="C46BA2E888304B43" modified="2020-08-12" version="1">MSLGTMHCTRCDEGFEPHERIVNSNGQLWHTQCFVCAQCFRQFQDGIFYEFEGRKYCEKDFHILFAPCCAKCNNFVIGRVIKAMAANWHPQCFTCERCSIPLADSGFIRNQNRALCHDCNRKEKEVGLGKLVCNKCHGIIDDAPLRFRGEVYHGYHFNCTSCGAELDSSAREVKNRSGYAANDMNELYCLRCHDRMGIPICGACRRPIEERVVTALGKHWHVEHFVCAKCEKPFLGHRHYEKRGMAYCETHYHQLFGNLCFVCNQVIAGDVFTALNKAWCVHHFSCSICDQKLDQKSKFFEYDEKPVCKKCYERFPSELRRRLRISHENTIKKPAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VG67</accession>
    <name>A0A6E8VG67_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RUN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VG67"/>
    <dbReference type="EnsemblMetazoa" id="ACON003431-RB">
        <property type="protein sequence ID" value="ACON003431-PB"/>
        <property type="gene ID" value="ACON003431"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006396"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003431"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_036733"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17681">
        <property type="entry name" value="RUN_RUFY1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.900:FF:000011">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047335">
        <property type="entry name" value="RUFY1-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004012">
        <property type="entry name" value="Run_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037213">
        <property type="entry name" value="Run_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45956">
        <property type="entry name" value="RUN AND FYVE DOMAIN-CONTAINING PROTEIN 2-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45956:SF6">
        <property type="entry name" value="RUN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02759">
        <property type="entry name" value="RUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00593">
        <property type="entry name" value="RUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140741">
        <property type="entry name" value="RUN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50826">
        <property type="entry name" value="RUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RUN" evidence="4">
        <location>
            <begin position="238"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="615"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="695"/>
            <end position="758"/>
        </location>
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            <begin position="428"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="522"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="15"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="705"/>
            <end position="723"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003431-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="758" mass="80177" checksum="908045719C65ECF2" modified="2020-08-12" version="1">MRALGIAQPNNHHQTTVASNNTTTSTSSSSSTTASNVGSVGASTGGSAAAGINGKNGNGDGHHHGPGATLITLPRAIPAGTGSAGDGHHRLPGGQLRTAGHGGVVAGGGSVSDTAAQDTIYLCNFRVTVDGEWLCLKELQDLDIKDGQAGGGGGGSGGADALGGGGGGAGGGTSDCNALNSFGIIENVKCEEKIIERDWVNYYSRDPASIERSNLVKICKLVVKELLEQSLRFGRMLDSDHLPLQHFFIVLEHVLRHGLKPKKGLLGPKKELWDILQSVERYCVEAMDITSSVRDLPTVRTHMGRARAWLRLALMQKKLADYLQVLIEHRDDSLAEYYEPHALMLSDEIIIIVGILVGLNVIDCNLCVKEEDLDSQQGVIDFSLYLRSSSRNSPDEENNGGVGGGINDSATGAMTAVLDQKNYIEELNRHLNATVANLQAKVESLTTTNALMKEDLAIARNSLMALQAENAALRRASQHGHPIEDKTNVLDRFDPEMAETLAEERKKRQEIEKELDLQVSLKAETEMAMKLLEKDIHEKQDTIVSLRRQLEDIKSINLDMYRKLQECELELTQKGEMVSRLQIKASQIGKILSNLERKGAADAMTDSQISTLNSFTGGGVSSPSGSGTYGGGLVGPPNKKPHLKIGTIPPFNANKLRKSPSVGASAAAAATTAAGSASSATTTTTTTATTATTAAAAATTAPAEGSNTTSKGVSGEGQQSVQDLQKRLSSPPKVDLGGKPSDSERTADLSSEAKLPSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VG69</accession>
    <name>A0A6E8VG69_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="5">BTB domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VG69"/>
    <dbReference type="EnsemblMetazoa" id="ACON003439-RC">
        <property type="protein sequence ID" value="ACON003439-PC"/>
        <property type="gene ID" value="ACON003439"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005866"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003439"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034718"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18315">
        <property type="entry name" value="BTB_POZ_BAB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000036">
        <property type="entry name" value="Mod(Mdg4), isoform H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051095">
        <property type="entry name" value="Dros_DevTransReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110">
        <property type="entry name" value="BTB DOMAIN TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110:SF92">
        <property type="entry name" value="MODIFIER OF MDG4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="4">
        <location>
            <begin position="32"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="118"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="192"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="287"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="392"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="192"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="222"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="294"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="398"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003439-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="524" mass="57253" checksum="67A72A66F71AC8B5" modified="2020-08-12" version="1">MADDEQFSLCWNNFNSNLSAGFHESLQRGDLVDVTLAAEGHLVKAHRLILSVCSPYFRKMFTQVPVNQHAFIFLKDVSHSALQDLIQFMYCGEVNVKQDALPAFISTAEALQIKGLTETGDSAPTHQSPAKEEPAAAAAVPVTTATISTATIPASPASQRAKVQRNRIQSYKLESEESGDDKVVHIQATTSHHVSAQSNLSSQKRTMPQRGLQSHASKRTKMSISASSDGLDTSDSTPAQVQTVQTVQIVKQIPAQVIEPEYIELPIESINPKAEPDYTDETAEIETVDAETEQEQKLSEHDQGDADDDGNYVEDDTYGDMAMGKYEESYLTEGEEGAKPGVSGFVDSYTSDGGNATEISTQDLALEVDVSNELTRKLPDVTLFKTNRAKAEKKKADSSAPASSLLLQQQQQPPASIALSDGSGKKFKKIASTVQVVKPNVDTVKMINNVRPNPDHIVKQLSNGHTLMKLVKTRVRGFCVSCIRKMHDPEYKKKLEKIITYCSACPASEWSCVNCFDESHGVGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VG73</accession>
    <name>A0A6E8VG73_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="8">BTB domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
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                <person name="Galvin B.D."/>
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    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
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            <end position="215"/>
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    </feature>
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            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
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            <dbReference type="ARBA" id="ARBA00023242"/>
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            <dbReference type="PROSITE" id="PS50097"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VG78</accession>
    <name>A0A6E8VG78_ANOCL</name>
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        <submittedName>
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        <name type="scientific">Anopheles coluzzii</name>
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            <taxon>Nematocera</taxon>
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            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
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                <consortium name="EnsemblMetazoa"/>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="8">Ngousso</strain>
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        <subcellularLocation>
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        </source>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VG79</accession>
    <name>A0A6E8VG79_ANOCL</name>
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        <submittedName>
            <fullName evidence="8">BTB domain-containing protein</fullName>
        </submittedName>
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        <name type="common">African malaria mosquito</name>
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            <taxon>Pterygota</taxon>
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            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
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        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
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                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_049605716.1"/>
    </dbReference>
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    <dbReference type="EnsemblMetazoa" id="ACON003439-RM">
        <property type="protein sequence ID" value="ACON003439-PM"/>
        <property type="gene ID" value="ACON003439"/>
    </dbReference>
    <dbReference type="GeneID" id="120949302"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005866"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003439"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034718"/>
    <dbReference type="Proteomes" id="UP001105220">
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    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
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        <property type="match status" value="1"/>
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    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="7">
        <location>
            <begin position="32"/>
            <end position="98"/>
        </location>
    </feature>
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        <location>
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        <location>
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        <location>
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        </location>
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</entry>
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</entry>
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    </evidence>
    <sequence length="474" mass="52078" checksum="D795C8718D20AAB2" modified="2020-08-12" version="1">MADDEQFSLCWNNFNSNLSAGFHESLQRGDLVDVTLAAEGHLVKAHRLILSVCSPYFRKMFTQVPVNQHAFIFLKDVSHSALQDLIQFMYCGEVNVKQDALPAFISTAEALQIKGLTETGDSAPTHQSPAKEEPAAAAAVPVTTATISTATIPASPASQRAKVQRNRIQSYKLESEESGDDKVVHIQATTSHHVSAQSNLSSQKRTMPQRGLQSHASKRTKMSISASSDGLDTSDSTPAQVQTVQTVQIVKQIPAQVIEPEYIELPIESINPKAEPDYTDETAEIETVDAETEQEQKLSEHDQGDADDDGNYVEDDTYGDMAMGKYEESYLTEGEEGAKPGVSGFVDSYTSDGGNATEISTQVKVMPIPMEFNTWLVVEKSVLQYTTTQRGRTMLIFSGYKFVENRQSKRNIFWRCARYVKHGCRAACVTSKNCAGNDQSIRLTGMTHSHPPEDTATARLTTHYPPVVAPTNAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VG84</accession>
    <name>A0A6E8VG84_ANOCL</name>
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        <submittedName>
            <fullName evidence="8">BTB domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
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            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VG84"/>
    <dbReference type="EnsemblMetazoa" id="ACON003439-RN">
        <property type="protein sequence ID" value="ACON003439-PN"/>
        <property type="gene ID" value="ACON003439"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005866"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003439"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034718"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Mod(Mdg4), isoform H"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="7">
        <location>
            <begin position="32"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="118"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
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            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="287"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="192"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="222"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="294"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003439-PN"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="515" mass="57109" checksum="B1FCA228EB151CAD" modified="2020-08-12" version="1">MADDEQFSLCWNNFNSNLSAGFHESLQRGDLVDVTLAAEGHLVKAHRLILSVCSPYFRKMFTQVPVNQHAFIFLKDVSHSALQDLIQFMYCGEVNVKQDALPAFISTAEALQIKGLTETGDSAPTHQSPAKEEPAAAAAVPVTTATISTATIPASPASQRAKVQRNRIQSYKLESEESGDDKVVHIQATTSHHVSAQSNLSSQKRTMPQRGLQSHASKRTKMSISASSDGLDTSDSTPAQVQTVQTVQIVKQIPAQVIEPEYIELPIESINPKAEPDYTDETAEIETVDAETEQEQKLSEHDQGDADDDGNYVEDDTYGDMAMGKYEESYLTEGEEGAKPGVSGFVDSYTSDGGNATEISTQDIRKAREGKQSPNVTHFQLKMDNERNPYFKLQLHGQPKQPKLKLNKSSAKTDNAHRSPTLTVRKFNKPVAVDANKIQYTNGRGNNVLMYDGHRYIKNNCYGGKMYWKCSKWHTNCKARAITSVSNPEQCVLKNAHNHDVPREDMASNIRKDWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VG86</accession>
    <name>A0A6E8VG86_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="8">BTB domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
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            <taxon>Metazoa</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
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            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
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                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VG86"/>
    <dbReference type="EnsemblMetazoa" id="ACON003439-RP">
        <property type="protein sequence ID" value="ACON003439-PP"/>
        <property type="gene ID" value="ACON003439"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005866"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003439"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034718"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="7">
        <location>
            <begin position="32"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="118"/>
            <end position="140"/>
        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        </location>
    </feature>
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            <end position="215"/>
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        </location>
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        </location>
    </feature>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
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        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003439-PP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="481" mass="52943" checksum="8E91C5D2D6F50BF3" modified="2020-08-12" version="1">MADDEQFSLCWNNFNSNLSAGFHESLQRGDLVDVTLAAEGHLVKAHRLILSVCSPYFRKMFTQVPVNQHAFIFLKDVSHSALQDLIQFMYCGEVNVKQDALPAFISTAEALQIKGLTETGDSAPTHQSPAKEEPAAAAAVPVTTATISTATIPASPASQRAKVQRNRIQSYKLESEESGDDKVVHIQATTSHHVSAQSNLSSQKRTMPQRGLQSHASKRTKMSISASSDGLDTSDSTPAQVQTVQTVQIVKQIPAQVIEPEYIELPIESINPKAEPDYTDETAEIETVDAETEQEQKLSEHDQGDADDDGNYVEDDTYGDMAMGKYEESYLTEGEEGAKPGVSGFVDSYTSDGGNATEISTQVGLGKWTIIATNSDGGGSDDDESEDNVAVFSRTMRGKEQLVYKGQPFVFEKLVLTTGGQSKKIWRCNQWWNQKCRARVYTIDDHITPLNRYHTHSDIVKRKQRVVKRDKTCDTGGPVDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VG87</accession>
    <name>A0A6E8VG87_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="9">BTB domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Tdpoz family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VG87"/>
    <dbReference type="EnsemblMetazoa" id="ACON003428-RA">
        <property type="protein sequence ID" value="ACON003428-PA"/>
        <property type="gene ID" value="ACON003428"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005998"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003428"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039396"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18345">
        <property type="entry name" value="BTB_POZ_roadkill-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.210.10:FF:000028">
        <property type="entry name" value="Speckle-type POZ protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000008">
        <property type="entry name" value="Speckle-type POZ protein-like a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.250.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.210.10">
        <property type="entry name" value="Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056423">
        <property type="entry name" value="BACK_BPM_SPOP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002083">
        <property type="entry name" value="MATH/TRAF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008974">
        <property type="entry name" value="TRAF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24413">
        <property type="entry name" value="SPECKLE-TYPE POZ PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24570">
        <property type="entry name" value="BACK_BPM_SPOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22486">
        <property type="entry name" value="MATH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00061">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50144">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026001159" evidence="6">
        <location>
            <begin position="24"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="MATH" evidence="8">
        <location>
            <begin position="74"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="7">
        <location>
            <begin position="243"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003428-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="417" mass="47012" checksum="B0760C9F5D2D7D4C" modified="2020-08-12" version="1">MCLPVYFMPYSLFSVFFRLPVVSDCPAGQTARVTSNLHSSSSTMANSRVPSPPLPEVNTPVAENWCYTQVKVVKFSYMWTINNFSFCREEMGEVLKSSTFSAGANDKLKWCLRVNPKGLDEESKDYLSLYLLLVSCNKSEVRAKFKFSILNAKREETKAMESQRAYRFVQGKDWGFKKFIRRDFLLDEANGLLPEDKLTIFCEVSVVADSVNISGQSNIIQFKVPECKLSEDLGTLFDNEKFSDVTLAVGGREFQVHKAILAARSPVFAAMFEHEMEERKQNRVAITDVDHEVLKEMLRFIYTGKAPNLDKMADDLLAAADKYALDKLKVMCEEALCVNLSVETAAETLILADLHSADQLKAQTIDFINTHATDVVETVGWKNMVATHPHLLNEAYRALATQQIPPIGPPRKRVKMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VG89</accession>
    <name>A0A6E8VG89_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="8">BTB domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VG89"/>
    <dbReference type="EnsemblMetazoa" id="ACON003439-RS">
        <property type="protein sequence ID" value="ACON003439-PS"/>
        <property type="gene ID" value="ACON003439"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005866"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003439"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034718"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18315">
        <property type="entry name" value="BTB_POZ_BAB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000036">
        <property type="entry name" value="Mod(Mdg4), isoform H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051095">
        <property type="entry name" value="Dros_DevTransReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007588">
        <property type="entry name" value="Znf_FLYWCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110">
        <property type="entry name" value="BTB DOMAIN TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110:SF92">
        <property type="entry name" value="MODIFIER OF MDG4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04500">
        <property type="entry name" value="FLYWCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="7">
        <location>
            <begin position="32"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="118"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="192"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="287"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="192"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="222"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="294"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003439-PS"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="569" mass="63175" checksum="9DED521DA756A18B" modified="2020-08-12" version="1">MADDEQFSLCWNNFNSNLSAGFHESLQRGDLVDVTLAAEGHLVKAHRLILSVCSPYFRKMFTQVPVNQHAFIFLKDVSHSALQDLIQFMYCGEVNVKQDALPAFISTAEALQIKGLTETGDSAPTHQSPAKEEPAAAAAVPVTTATISTATIPASPASQRAKVQRNRIQSYKLESEESGDDKVVHIQATTSHHVSAQSNLSSQKRTMPQRGLQSHASKRTKMSISASSDGLDTSDSTPAQVQTVQTVQIVKQIPAQVIEPEYIELPIESINPKAEPDYTDETAEIETVDAETEQEQKLSEHDQGDADDDGNYVEDDTYGDMAMGKYEESYLTEGEEGAKPGVSGFVDSYTSDGGNATEISTQGHSSCSTVQHQHQGRLVHAKLNLVQRRHPLPSPVVKFKPAQKLMLPSSISVSRVLNTHDAKQQQHTTPPTRPIYTPVSDTVKFIRSQKKCAQLVYDGYIYNRKMIQQNGRTTWRCCDLLKYHCKATCVTKQNKLIGIRSEHNHNDHSSKIEDKALYDFPEDLEEYVNIRTRDPIDVKNHKVDVIDTGAEFKIVVRDRSSTMHQAAKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VHM6</accession>
    <name>A0A6E8VHM6_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="6">PDZ domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VHM6"/>
    <dbReference type="EnsemblMetazoa" id="ACON003937-RB">
        <property type="protein sequence ID" value="ACON003937-PB"/>
        <property type="gene ID" value="ACON003937"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007039"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003937"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031607"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031941">
        <property type="term" value="C:filamentous actin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001725">
        <property type="term" value="C:stress fiber"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051371">
        <property type="term" value="F:muscle alpha-actinin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
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        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
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                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
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        </source>
    </evidence>
    <sequence length="2059" mass="226699" checksum="087B876A24B64EE5" modified="2020-08-12" version="1">MSLNEKRMFDRETLRSVIQQWNTVRLDIFALSEPNENLEFHGVMRFYFQDEGQKVATKCIRVASDATVSDVIETLIEKFRPDMRMLSLPNYALYVVHANGEERKLNPDEKPLLVQLNWHNDDREGRFLLKNSHPDLFQQHSGLSALQLVACALAFKRKLSKREKKEQKKKEKLNKLQSAGGGTGGPDSENHVAEKLYTELPETSFTRSISNPEAVMRRRRQQKLEKKLQQFRSRDGGPDTGGTLKIYGESLCRDVPYKTLLLSIRDCAQAVVREMLSKYGMDKVDPLHYCLVQVRFSPYVNSDGSEYILDDDECPLSILMNHPTSRGSIMFHVRRRPADSQPRRRKKKPLGVSNGGNNIGGDREGPMLIEITHSGDGGRRVKLGSEPIEVGSANTNALQLFGPSIQARHCLISMHDGVCTVTPLHADGTTFVNGHHIQQPTILHNGSVVMFGRVASYRFVDSPSDGRYNLALSQSQLDSACLYESRSPTSPTSWNDEESRPISPISTVQMGGKLPGGGGGGSFLDGPIASTSAESATAAQQQANAFAKSASFDMQSATAAQPHPHMHHHHHHHPSGLLVTALDGPEAMGADDTASYKGGHAGGTNDHNEGEQQQHHAGGGGPLGAGNGGETETKSISSFKSIGSTADRTSTFPKLQPSHAATSISGEPGGQEPILPAVLEFPEQNQEPFLQSVISELDVNSPNFKLAPVYTLYLCARYRASTHYRPDLQPTERAHKLTVFLHHVANLIQNVVQEQYTDAKILSFWMANSSEFLHFLKSDRHISAFSVQAQEVLAESVQTAFRNLVSIFHVELSQTLNQFLSENIDHDSAAGLVLSVLGSAMALLRRCRVNAALTIQLFSQLFHYINVVCFNKFVTTSHMCTSAWGKALSERLSLLELWAEKQGLELAADCHLAKINQCAQFLQAPKTSVSDVQQLACSCFRLNSLQMAALLSQETIPRNLIDTAVRMAESVADELSRADGREIRLEESPELPLALLLPDDGFSCDVVRGIPAGLVDFLNPFQMAGWCRLASQPTSIGLWTVYMHQFNHGRSPSVMSSKLPQPEVQIIKLHKNANGMGLSIVAAKGAGQERLGIYIKSVVGGGAADLDGRLQAGDQLLEVDGQSLIGITQERAADHLVRTGPIVTLKVAKQGAIYHGLATLLQQPSPVIQREMENEFNCNVYDNDGNHRYHFGRSNESLNFYGSGGGSHQSRRGSLRRPLSSVMMNHTYSTPYLTQNDAAYRRMSNPDISQRRYSLSLSHEDILSNDQLQQIHFDPRYRDCRSSNSLNSIYYNQQRSRSNSLNSIGRIGTGIKPVRYDTPPSIYVEDYDSRSNSHSSREMIKIEETAEPETPVIEERRPSCVFEIRIEAPSTESNLSFVADVNLDDIPFIDEDDADEEDAPGSRSRGAGDSSGVNVSTQTAAATPSVVVNPPSSSSSTATAGVSTNHSVNSNITAVGGGPLPSIMINTASRRGSNASCRKTVSFDIINDRHPYDDSEEGDRANRKQYNNGQSNVENNVATNLQTDAWSAAAAVPANSRVSNRLNNNQIFIDNIPAEHSEVATFVGPFQSVLHSSGGIAKGFDGIAGDVESNNNGQSVEDPHNTIARTTNATGGESRAQERPIDTIRDYVVRHFSGAANINVNNNYYCSNSVDRRQRNIKLDSDDETTTTSVYPQDVSTPSEQEPRKSGSNGDDRLVDNRTGAHFPTDSNYAPSATLDNDHCNLLRNIQLLKADAAFVAPVEHNDSNGDPGNNDPHHHHHPHHHHLHHTGQGGAKVSKPPETTSSCFGTAPEEMNTSHPDGTIKFGQGKVKALTKFYDSLKDTNNNPIKKYAKPHDCTDSPARHVRKYEIIEPMNCPDDITANHLSKDEERQIMNQLQLWSKFGSDSEELHLAPVSSFRRSQSCDTLLNDPPEGEGNNNVEEHCELSFERLDSGNNPYGCKRTATKASPNSSDLHAAYHGLHPHTLPSGFLLRKVKKCHPSCKNIENQKVTLSFLYGRNSPPVEQQQHHRTSCPDLSSIRQTPRQLKKQHNILRLLKHINNKLGDASDSESMPPTANEEDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VHZ6</accession>
    <name>A0A6E8VHZ6_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="8">DM domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VHZ6"/>
    <dbReference type="EnsemblMetazoa" id="ACOM043176-RB">
        <property type="protein sequence ID" value="ACOM043176-PB"/>
        <property type="gene ID" value="ACOM043176"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON004050-RA">
        <property type="protein sequence ID" value="ACON004050-PA"/>
        <property type="gene ID" value="ACON004050"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012676"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004050"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039146"/>
    <dbReference type="Proteomes" id="UP000075882">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007548">
        <property type="term" value="P:sex differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1040.10:FF:000001">
        <property type="entry name" value="doublesex- and mab-3-related transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1040.10">
        <property type="entry name" value="DM DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001275">
        <property type="entry name" value="DM_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036407">
        <property type="entry name" value="DM_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026607">
        <property type="entry name" value="DMRT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014932">
        <property type="entry name" value="DSX_dimer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12322">
        <property type="entry name" value="DOUBLESEX AND MAB-3 RELATED TRANSCRIPTION FACTOR DMRT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12322:SF100">
        <property type="entry name" value="PROTEIN DOUBLESEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00751">
        <property type="entry name" value="DM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08828">
        <property type="entry name" value="DSX_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00301">
        <property type="entry name" value="DM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01143">
        <property type="entry name" value="DSX_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82927">
        <property type="entry name" value="Cysteine-rich DNA binding domain, (DM domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS40000">
        <property type="entry name" value="DM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50809">
        <property type="entry name" value="DM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="DM" evidence="7">
        <location>
            <begin position="41"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="DM" evidence="5">
        <location>
            <begin position="41"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="132"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004050-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="265" mass="29618" checksum="EFF8337C350D2E11" modified="2020-08-12" version="1">MVSQDRWTEAMSDSGYDSRTDGNGAASSCNNSLNPRTPPNCARCRNHGLKIGLKGHKRYCKYRACQCEKCCLTAERQRVMALQTALRRAQTQDEQRALNEGEVPPEPVANIHIPKLSELKDLKHNMIHNSQPRSFDCDSSTGSMASAPGTSSVPLTIHRRSPGVPHHVPEPQHMGATHSCVSPEPVNLLPDDELVKRAQWLLEKLGYPWEMMPLMYVILKSADGDVQKAHQRIDEGQAVVNEYSRLHNLNMFDGVELRNTTRQSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VI96</accession>
    <name>A0A6E8VI96_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="6">PDZ domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VI96"/>
    <dbReference type="EnsemblMetazoa" id="ACON003937-RC">
        <property type="protein sequence ID" value="ACON003937-PC"/>
        <property type="gene ID" value="ACON003937"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007039"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003937"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031607"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031941">
        <property type="term" value="C:filamentous actin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001725">
        <property type="term" value="C:stress fiber"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051371">
        <property type="term" value="F:muscle alpha-actinin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061061">
        <property type="term" value="P:muscle structure development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000055">
        <property type="entry name" value="PDZ and LIM domain protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050604">
        <property type="entry name" value="PDZ-LIM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24214">
        <property type="entry name" value="PDZ AND LIM DOMAIN PROTEIN ZASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24214:SF38">
        <property type="entry name" value="PDZ AND LIM DOMAIN PROTEIN ZASP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0440">LIM domain</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PDZ" evidence="5">
        <location>
            <begin position="25"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="102"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="193"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="143"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="193"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="233"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="275"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003937-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="288" mass="31728" checksum="97EFA4A3F3385E9A" modified="2020-08-12" version="1">MPEFVIEKRNIPVLTVSSRDGPAASENGDNPLGFRITGGADFEMPITVFQVSEGSPAQKAGLQLGDQILKINGADASAMRLATAQSVIKQAGEQLQMIVAKDDENNRKAEEQGQPKETREVKFAGNEQEPAADVTNKPAPQPKNDERENWTQPPERKVWHPIVWQQPPPPIPPDLYGKDAPHQQLIANIRRLLTETRTKPEERQKHIERMMLSLPTGSRREGEEDEEEPKPAPKPKKKKKSKAKKAKAAPRTRLDSETSEDGSVSELTGPAAAEPAEEPIPEEASVEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VI99</accession>
    <name>A0A6E8VI99_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glutathione transferase</fullName>
            <ecNumber evidence="3">2.5.1.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">GST class-theta</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GST superfamily. Theta family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8VI99"/>
    <dbReference type="EnsemblMetazoa" id="ACON004164-RD">
        <property type="protein sequence ID" value="ACON004164-PD"/>
        <property type="gene ID" value="ACON004164"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001564"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004164"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039141"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03177">
        <property type="entry name" value="GST_C_Delta_Epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03045">
        <property type="entry name" value="GST_N_Delta_Epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000034">
        <property type="entry name" value="glutathione S-transferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000007">
        <property type="entry name" value="Glutathione S-transferase 1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43969">
        <property type="entry name" value="GLUTATHIONE S TRANSFERASE D10, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43969:SF9">
        <property type="entry name" value="GLUTATHIONE S TRANSFERASE D10, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01153">
        <property type="entry name" value="Main.4:_Theta-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="7">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="8">
        <location>
            <begin position="92"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00041523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004164-PD"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="183" mass="20856" checksum="84F819B3A9BB9259" modified="2020-08-12" version="1">MDFYYLPGSAPCRAVQMTAAAVGVELNLKLTDLMKGEHMKPEFLKLNPQHCIPTLVDEDGFVLWESRAIQIYLVEKYCAHDPALAERLYPGDPRRRAVVHQRLFFDVAILYQRFAEYYYPQIFGKKVAGDPDRLRSMEQALEFLNTFLEGERFVAGGDDPTIADFSILADCNVRSCRVRSAAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VJQ0</accession>
    <name>A0A6E8VJQ0_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Neuropeptide F</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_040224122.1">
        <property type="nucleotide sequence ID" value="XM_040368188.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VJQ0"/>
    <dbReference type="SMR" id="A0A6E8VJQ0"/>
    <dbReference type="EnsemblMetazoa" id="ACON004642-RA">
        <property type="protein sequence ID" value="ACON004642-PA"/>
        <property type="gene ID" value="ACON004642"/>
    </dbReference>
    <dbReference type="GeneID" id="120950278"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_013354"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004642"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042054"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026134226" description="Neuropeptide F" evidence="1">
        <location>
            <begin position="30"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004642-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="89" mass="9838" checksum="A893F9996208BEA1" modified="2020-08-12" version="1">MASGTFTQRLLVALMIFALIADLSTLVAARPQDSDAASVAAAIRYLQELETKHAQHARPRFGKRGGYLNPAIFGQDEQEVDWQDSTFSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VKA2</accession>
    <name>A0A6E8VKA2_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Formin-like protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the formin homology family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VKA2"/>
    <dbReference type="EnsemblMetazoa" id="ACON004805-RA">
        <property type="protein sequence ID" value="ACON004805-PA"/>
        <property type="gene ID" value="ACON004805"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006415"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004805"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_043281"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030866">
        <property type="term" value="P:cortical actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2220">
        <property type="entry name" value="Formin, FH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015425">
        <property type="entry name" value="FH2_Formin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042201">
        <property type="entry name" value="FH2_Formin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010472">
        <property type="entry name" value="FH3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043592">
        <property type="entry name" value="FMNL_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014768">
        <property type="entry name" value="GBD/FH3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010473">
        <property type="entry name" value="GTPase-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45857">
        <property type="entry name" value="FORMIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45857:SF4">
        <property type="entry name" value="FORMIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06367">
        <property type="entry name" value="Drf_FH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06371">
        <property type="entry name" value="Drf_GBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02181">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01139">
        <property type="entry name" value="Drf_FH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01140">
        <property type="entry name" value="Drf_GBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00498">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51444">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51232">
        <property type="entry name" value="GBD_FH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GBD/FH3" evidence="4">
        <location>
            <begin position="85"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="domain" description="FH2" evidence="5">
        <location>
            <begin position="659"/>
            <end position="1059"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="222"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="566"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="448"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="945"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="231"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="584"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="624"/>
            <end position="641"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004805-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1116" mass="125538" checksum="892EEA7E5524EB1F" modified="2020-08-12" version="1">MGLASSKAVSSIAVSSSHAAGSTDKGNGRLSKDSVIVETNYASMSTDIQQPCSILPSPSGSSGIDTWKTSSCHVRQASIRSRNLQPMPDAGELDRRFAKVLASMDLPPDKAKLLKNYDNEKKWDIICDQEMVHAKDPPAHYLTKLRTYLDPKASRSHRKRKMVGESTSTQVLRDLEISLRTNHIEWVKEFLDEENQGLDALIDYLSFRLTMMRHEQRILEAKSESDEGLTTKETAANSSYGSNETNHKIAPNGFMRPGLGDMLDSPSIKRRSRHIAKLNMGLTTDDIHVCIMCMRAIMNNKYGFNMVIQHREAINCIALSLIHKSLRTKALVLELLAAICLVKGGHEIILCAFDNFKKVCSEQRRFQTLMEYFMNYELFNIDFMVACMQFVNIVVHSVEDMNYRVHLQYEFTALGLDEYLEKLRLTESEELHVQISAYLDNVFDVAALMEDSETKTAALERVNELEDELGRALDQASEIEREALFKIGELEAELSRTRNERDDLYNKQLVVEDEIVKLKRALKQHEQESQSRQSMLLELENLTKTLPKGTSIADVSNLLAKGGLSELSPTGAAGTGAGGQQQAPPIPPPPAPPIPPSAANAPMPPPPPCPPAPPSGLSNGGEPPKAPPKPPSFIPVPPPMGGPYGGNNALHCTDGAMTIKRKVQPKYKLPTLNWVALKPNQVRGTIFNELDDEKLHRQIDFVDFEERFKIGMGGPVTNGNCDMDGLTAFPSKRFKKPEHISLLEHTRLRNIAISRRKLEMPAETVIKAINNLDLKLLSLENVELLQKMTPTDQEQKLYKEYVIEKKDLNQLTEEDKFMLQLTKVERISSKLSIMNYIGNFFESWHLISPQVYSIISASSSIKSSKKFRAVLEVILAFGNYLNSSKRGPAYGFKLQSLDTLLDTKSNDKRMSLMHYIVATIRQKFPELMNFDTELFCIDKAAQVSLEMLISDVNELEKGMETVRKEADLRGKGTQSHVLRDFLANSEEKLKKIRSDCKTAQESFKECIEYFGESSRNADANAFFSLLVRFVRAFKICDQENEQRRRLEAAALQASTKKDEEEQVVLRNNKINNQKKQQVRPLADGNPLRLSTAFLQNLTQMNRELQHVFGSGSSYVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VLU1</accession>
    <name>A0A6E8VLU1_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">phosphatidylinositol-3,5-bisphosphate 3-phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.95</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Phosphatidylinositol-3,5-bisphosphate 3-phosphatase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein-tyrosine phosphatase family. Non-receptor class myotubularin subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.95" evidence="4"/>
    <dbReference type="RefSeq" id="XP_040221387.1">
        <property type="nucleotide sequence ID" value="XM_040365453.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VLU1"/>
    <dbReference type="EnsemblMetazoa" id="ACON005341-RA">
        <property type="protein sequence ID" value="ACON005341-PA"/>
        <property type="gene ID" value="ACON005341"/>
    </dbReference>
    <dbReference type="GeneID" id="120948754"/>
    <dbReference type="CTD" id="9107"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010700"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005341"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_036666"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052629">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate 3-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004438">
        <property type="term" value="F:phosphatidylinositol-3-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15738">
        <property type="entry name" value="FYVE_MTMR_unchar"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13210">
        <property type="entry name" value="PH-GRAM_MTMR6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14532">
        <property type="entry name" value="PTP-MTMR6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000135">
        <property type="entry name" value="Myotubularin related protein 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030564">
        <property type="entry name" value="Myotubularin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010569">
        <property type="entry name" value="Myotubularin-like_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048994">
        <property type="entry name" value="PH-GRAM_MTMR6-9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10807">
        <property type="entry name" value="MYOTUBULARIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10807:SF8">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-3-PHOSPHATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06602">
        <property type="entry name" value="Myotub-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21098">
        <property type="entry name" value="PH-GRAM_MTMR6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51339">
        <property type="entry name" value="PPASE_MYOTUBULARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Myotubularin phosphatase" evidence="18">
        <location>
            <begin position="124"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="16">
        <location>
            <begin position="304"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="FYVE-type" evidence="17">
        <location>
            <begin position="627"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphocysteine intermediate" evidence="13">
        <location>
            <position position="334"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="272"/>
            <end position="273"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="334"/>
            <end position="340"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR630564-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR630564-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005341-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="691" mass="79292" checksum="8826C275461EE997" modified="2020-08-12" version="1">MEHINTPKIENVRMIDRYNSKNPTVGVLYLTATHLIFVDPDANKETWVLYMHIANVEKLPLSTTGSPLLIRCKTFLSITFVIPKERECHDVYTTLTKLYQPVNIKNLYCFQYTTAAKELPKAAGWDYFKLEHEFKRMRVPNDQWSVCALNQNYELCDTYPQQLYVPAEANTQILLGSSRFRSKGRLPALTYLHSNKASICRCSQPLSGFSARCLEDEQMLETVRKTNPNCNFMYVVDTRPRINAMANRAAGKGYENEANYENIKFQFLGIENIHTMRASLQKVIECCEQKSPTMSGFLSALESSGWLKHIRSILDTSCFIANAVDKGISVVVHCSDGWDRTAQVCSLAALMLDPYYRTIKGFQALIEKDWLAFGHKFSDRCGHIQNDPKEVSPVFTQLLECTWQLMQQRNDAFEFNERFLLILHDHVMSCQYGTFVGNCEKDRLDLRLAEKTFSLWGYMSNHLNEYINPLYRPEMDETIKPNLAPQNIKFWRGMYSRFESGIHPREPLEDLLIASKDHCTSLEDHVQHLTKRINSFKNMLSKSAKRLQGGSAALQSRYNADTRGPIEVNDNRYNYDRKLSELSAADDDHPLKTSDFSFSNLSISEYNNEVEKVNDDINSVAVEWKSLRSVVNCPTCSIPFDQITKKNHCWKCGEVFCSRCIDKTLALPGHDSGNPVPVCRQCFRVVQQVSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VM60</accession>
    <name>A0A6E8VM60_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="12">SH3 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Focal adhesion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CAS family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VM60"/>
    <dbReference type="EnsemblMetazoa" id="ACON005411-RB">
        <property type="protein sequence ID" value="ACON005411-PB"/>
        <property type="gene ID" value="ACON005411"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004428"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005411"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033722"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11564">
        <property type="entry name" value="FAT-like_CAS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11549">
        <property type="entry name" value="Serine_rich_CAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.830:FF:000001">
        <property type="entry name" value="BCAR1 scaffold protein, Cas family member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.230:FF:000001">
        <property type="entry name" value="Breast cancer anti-estrogen resistance 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.230">
        <property type="entry name" value="Alpha-catenin/vinculin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.830">
        <property type="entry name" value="Serine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021901">
        <property type="entry name" value="CAS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037362">
        <property type="entry name" value="CAS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014928">
        <property type="entry name" value="Serine_rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038319">
        <property type="entry name" value="Serine_rich_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10654">
        <property type="entry name" value="CAS SCAFFOLDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10654:SF18">
        <property type="entry name" value="IP17195P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12026">
        <property type="entry name" value="CAS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08824">
        <property type="entry name" value="Serine_rich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="8" id="KW-0965">Cell junction</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="Serine rich protein interaction" evidence="10">
        <location>
            <begin position="133"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="CAS family C-terminal" evidence="11">
        <location>
            <begin position="326"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="295"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="70"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="295"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="312"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF12026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005411-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="519" mass="55660" checksum="AB026C7C4C9E0ECF" modified="2020-08-12" version="1">MEESYDVPRPLLSQQQHLTPSSSNSSLLTSDSFSLSSSNRSSLANMPDYDVPRRNPVSVRSTPPPPATPGPQSGAPAPGTPSFTASHHSMSMSSFSSMSTYDVPPSHGGGPGYVSAKLHQQQTAPATPKELPLELSSALETLARLQNEATTAVTRLLSFVSPNWRVKEKLDPILMDLKLAAVRLRTALHDLAEFGDGALGNASKAEDKGLAQKLKPLVKALRDADKLVHEASQNLDAQGWTLVTLQRPEATQKLQQQLPPDSLDQLIACAQTLTEDVRQTASFIQGNATLLFKRSTSTPQSSVPTTPQTPTGAGGKPSTGGSGGGGEWPEDYDYVSLESKEASARTNAEIRDALPNDLKKNFENVVRNAADAAAASVSERPDVALDPNDKSVLVYYASQTVTHMGYLTQAIDAFLQTVEHNQPPKFFLAYGKFVVLSAHNLVNIGDIVHRNVSRECIKTRVLSCADALSEALKTCVAKTKKAAQNFPSVTAVQEMVDSVVDISHLASDLKIAMLEAVQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VM61</accession>
    <name>A0A6E8VM61_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VM61"/>
    <dbReference type="EnsemblMetazoa" id="ACON005378-RC">
        <property type="protein sequence ID" value="ACON005378-PC"/>
        <property type="gene ID" value="ACON005378"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011259"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005378"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033496"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000181">
        <property type="entry name" value="CALML5 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050145">
        <property type="entry name" value="Centrin_CML-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23050">
        <property type="entry name" value="CALCIUM BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="9"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="45"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="103"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="139"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005378-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="181" mass="20615" checksum="1EFB37D80B813DC9" modified="2020-08-12" version="1">MSQNFINLSEIRDLRTAFDLLDRDQDGHVTPEELQFMLRNLGIHVRDELIDDLLREASRTGSGLIDETEFLQWVARIQALKDDSNTSSSSSSSNNPAQAADDDLTQDLVAAFRVFDRDGNGYITRDELKSAMDMIGENVTEYQLNEMLELADADKDGRINYEDFQNFFLNNFDALVQPPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VM62</accession>
    <name>A0A6E8VM62_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phospholipid scramblase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">May mediate accelerated ATP-independent bidirectional transbilayer migration of phospholipids upon binding calcium ions that results in a loss of phospholipid asymmetry in the plasma membrane.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the phospholipid scramblase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040229881.2">
        <property type="nucleotide sequence ID" value="XM_040373947.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VM62"/>
    <dbReference type="EnsemblMetazoa" id="ACON005504-RA">
        <property type="protein sequence ID" value="ACON005504-PA"/>
        <property type="gene ID" value="ACON005504"/>
    </dbReference>
    <dbReference type="GeneID" id="120953737"/>
    <dbReference type="KEGG" id="acoz:120953737"/>
    <dbReference type="CTD" id="326186"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004426"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005504"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038722"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017128">
        <property type="term" value="F:phospholipid scramblase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005552">
        <property type="entry name" value="Scramblase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025659">
        <property type="entry name" value="Tubby-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23248:SF9">
        <property type="entry name" value="PHOSPHOLIPID SCRAMBLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23248">
        <property type="entry name" value="PHOSPHOLIPID SCRAMBLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03803">
        <property type="entry name" value="Scramblase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54518">
        <property type="entry name" value="Tubby C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0564">Palmitate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="36"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU363116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005504-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="340" mass="37261" checksum="CC768FA55678B6C0" modified="2020-08-12" version="1">MNPQQQPQAPYAPYAPQYQGYTDQPGYPPAGGGGYPPAPYPPPGTQSPPAGYWGSPYPPAAPGYGQPPQMYAPNQPYGGGGYQPVMVQPGMPGHPPPVMGQPVPGGWMPIPQGIPNCPPGLEYLTAIDQLLVHQKVELLEAFTGFETANKYTVKNTLGQKVYWAMEDTGCCNRMCCGAARAFDMKILDTYQNEVLHFNRPLRCSSCWFPCCLQTMEVTAPPGNVIGYVEQDWSILTPQFSIKNQNGETVLKISGPFCTFSICGDVEFEVLSTNGTQVGKISKQWSGLGREMFTDADHFGINFPMDLDVRVKATLLGALFLIDYMFFEKSGNKEQDRPGMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VMA3</accession>
    <name>A0A6E8VMA3_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the gemin-2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VMA3"/>
    <dbReference type="EnsemblMetazoa" id="ACON005413-RB">
        <property type="protein sequence ID" value="ACON005413-PB"/>
        <property type="gene ID" value="ACON005413"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_003360"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005413"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041547"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032797">
        <property type="term" value="C:SMN complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000387">
        <property type="term" value="P:spliceosomal snRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035426">
        <property type="entry name" value="Gemin2/Brr1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12794:SF0">
        <property type="entry name" value="GEM-ASSOCIATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12794">
        <property type="entry name" value="GEMIN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04938">
        <property type="entry name" value="SIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="226"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00025758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005413-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="249" mass="28049" checksum="A8A21278C6D288B3" modified="2020-08-12" version="1">MEVDTIQKPALAVEPPDANFDPNLTPETGEQYLQKVMYERGKCPVVVVAAEKPQPRRQQKALSGLAAVPTDDVKNVAHRALIPTREWETMQNQKFSELRDTITAYRSSAQYEENLQRTHVYLNFDDRKHLHEYCANNLPYVSILLSIPQRNLEILLEYLHEWLQDSDQADTVHVGQGAVAARRWLPPGLDTAVDLRHTRLSDQTARAVHSQRAAGHCQNVHHAAQRAEAGGRGEGTAAQSTDQHHFEAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VMB6</accession>
    <name>A0A6E8VMB6_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="3">RPGRIP1 C-terminal domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VMB6"/>
    <dbReference type="EnsemblMetazoa" id="ACON005468-RB">
        <property type="protein sequence ID" value="ACON005468-PB"/>
        <property type="gene ID" value="ACON005468"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004702"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005468"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031263"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035869">
        <property type="term" value="C:ciliary transition zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905515">
        <property type="term" value="P:non-motile cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031139">
        <property type="entry name" value="RPGRIP1_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14240:SF1">
        <property type="entry name" value="PROTEIN FANTOM-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14240">
        <property type="entry name" value="RETINITIS PIGMENTOSA GTPASE REGULATOR-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="143"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="350"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="15"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="158"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="176"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="223"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="256"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="302"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="314"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="334"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005468-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="583" mass="67304" checksum="F99502B6A2B75006" modified="2020-08-12" version="1">MGKDSVREKDLPGMAAAATATSGGRRSGRPVSASPYSEHYRPPIDLCQRHLINHRLLVSKLTRRELEDKYLSLCDENYTIKKRFLEQEELIKRLKTKLSRLSNESNQRSKSRLDMASSEHYSRLHDLELQRRELHEKLEALRRATSNDGRMRENSVCKARQSAPASSQRRSKSARPAKETAGGRRECKSTSPSADGYDEREERLRHRLTRQAGGKGRTSTDDRTEDDEEEEEEEEEEESHAVAAARRSSHRKSYGADDEEEEEEEEDEDSDDKDVRETLDEDRDVLSGGGSEELNESERDSPASGAAGGAKSISKGRRHAAKSKGRQAAGAHCPDCERHRAEQVTRETDLVKMKLNIKYLHKELQNEKEKSTLLARQLEEKLSYEIMKRNAAENLEILNLNRQVEDLAKELQRQVDEQKRSMEHELRKQGDLEAQIRKEKDKNASLFEECERLKKNIEKLKENMSEVEIERDFLKRQQENFTKIVDENKLLKYQLDELRKHNEELLRQIDTLREEELVTKESQRELLEKLKTLQQDNDTLSVMLEGLRTENVLLAEERTQLEQSLKSLEASPIKEVRPVSPVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VMC7</accession>
    <name>A0A6E8VMC7_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="13">BTB domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Putative transcription factor required for axon growth and guidance in the central and peripheral nervous systems. Repels CNS axons away from the midline by promoting the expression of the midline repellent sli and its receptor robo.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VMC7"/>
    <dbReference type="EnsemblMetazoa" id="ACON005245-RG">
        <property type="protein sequence ID" value="ACON005245-PG"/>
        <property type="gene ID" value="ACON005245"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009842"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005245"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_029613"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016199">
        <property type="term" value="P:axon midline choice point recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048813">
        <property type="term" value="P:dendrite morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008406">
        <property type="term" value="P:gonad development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035167">
        <property type="term" value="P:larval lymph gland hemopoiesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007526">
        <property type="term" value="P:larval somatic muscle development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045476">
        <property type="term" value="P:nurse cell apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007464">
        <property type="term" value="P:R3/R4 cell fate commitment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045467">
        <property type="term" value="P:R7 cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18315">
        <property type="entry name" value="BTB_POZ_BAB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000091">
        <property type="entry name" value="Lola, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051095">
        <property type="entry name" value="Dros_DevTransReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110">
        <property type="entry name" value="BTB DOMAIN TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110:SF111">
        <property type="entry name" value="LONGITUDINALS LACKING PROTEIN, ISOFORMS F_I_K_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13909">
        <property type="entry name" value="zf-H2C2_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3" id="KW-0221">Differentiation</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="11">
        <location>
            <begin position="32"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="553"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="115"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="301"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="462"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="161"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="175"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="212"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="322"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="476"/>
            <end position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005245-PG"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="634" mass="66865" checksum="7A91F71CAFA59761" modified="2020-08-12" version="1">MDDDQQFCLRWNNHQSTLISVFDTLLENGTLVDCTLAAEGKLLKAHKVVLSACSPYFATILSQQYDKHPIFILKDVKFQELRAMMDYMYRGEVNISQDQLAALLKAAESLQIKGLSDNRSSSSSGTAAAGGGVHQKQSDAPAAKTLQPPVPASKASGLTIENKRSLKSELLESDVSGSREGSTSPTSRKRKKVRRRSVDTNNLLIDNHDQHSNSSSHSMHTAGGANVAAAVAAAGAPAANVASSLSSASSTAAAGSNTSANVLSVAAVAAAAVLKKTETVQQAAAETLKLQKQQAAAAAAAAAAQSSDDEERCGPGSVATGTEHDDGNDLSDNEHDGDGGSSVKSGSRHHHLRDGSRRGKTSTSSMGEMMIEPKSEYEDVQDENVEDLTMDDEDLMDELDQAGPSHGGDVSTEDLSITKLGGLTWDQLDARLFAGSAGALLTAAGGAMPLVTTLREGPHPLVFPRDHSVSPVNVKSTSSASSSSSSGGTGASNNGSNSGISMLNNNNNNNTTSNNNNNHHRSGSSAAGDVGGNSSNSMSVSSLHCAELSGCGFACPDCGRTYKLKSSLRNHQKWECGKEPQFQCPYCVYRAKQKMHIGRHMERMHKERYFKMEGDKVIALEDPTTLALDLNQTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VMD0</accession>
    <name>A0A6E8VMD0_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the BI1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VMD0"/>
    <dbReference type="EnsemblMetazoa" id="ACON005529-RA">
        <property type="protein sequence ID" value="ACON005529-PA"/>
        <property type="gene ID" value="ACON005529"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010512"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005529"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032939"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd10428">
        <property type="entry name" value="LFG_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006214">
        <property type="entry name" value="Bax_inhibitor_1-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23291">
        <property type="entry name" value="BAX INHIBITOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23291:SF47">
        <property type="entry name" value="TRANSMEMBRANE BAX INHIBITOR MOTIF CONTAINING 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01027">
        <property type="entry name" value="Bax1-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="34"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="68"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="97"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="123"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="154"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="178"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="215"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005529-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="241" mass="26759" checksum="31FAD40A65767456" modified="2020-08-12" version="1">MPGAYGAAYDPETGSVKGFEFNDQSIRRGFIRKVYSILTVQLAITLGFITLFLYHRPTKLWVQNHPEMFWIALGVMVVTLISMACCGDVRRKAPMNFIFLTLFTLAQAFLLGVTTANFSSQEVMLAVGITAAVCLGLTLFAFQTKWDFTVMGGILFVAVLILMLFGLIAIFFPGKTITLVYASAGALIFSIYLVYDTQLMLGGEHKYSISPEEYIFAALNLYLDIVNIFLYILTIIGASRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VMD3</accession>
    <name>A0A6E8VMD3_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="13">BTB domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Putative transcription factor required for axon growth and guidance in the central and peripheral nervous systems. Repels CNS axons away from the midline by promoting the expression of the midline repellent sli and its receptor robo.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VMD3"/>
    <dbReference type="EnsemblMetazoa" id="ACON005245-RL">
        <property type="protein sequence ID" value="ACON005245-PL"/>
        <property type="gene ID" value="ACON005245"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009842"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005245"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_029613"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016199">
        <property type="term" value="P:axon midline choice point recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048813">
        <property type="term" value="P:dendrite morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008406">
        <property type="term" value="P:gonad development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035167">
        <property type="term" value="P:larval lymph gland hemopoiesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007526">
        <property type="term" value="P:larval somatic muscle development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045476">
        <property type="term" value="P:nurse cell apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007464">
        <property type="term" value="P:R3/R4 cell fate commitment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045467">
        <property type="term" value="P:R7 cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18315">
        <property type="entry name" value="BTB_POZ_BAB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000091">
        <property type="entry name" value="Lola, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051095">
        <property type="entry name" value="Dros_DevTransReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110">
        <property type="entry name" value="BTB DOMAIN TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110:SF111">
        <property type="entry name" value="LONGITUDINALS LACKING PROTEIN, ISOFORMS F_I_K_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3" id="KW-0221">Differentiation</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="11">
        <location>
            <begin position="32"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="481"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="115"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="301"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="507"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="161"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="175"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="212"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="322"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="515"/>
            <end position="543"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005245-PL"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="552" mass="60368" checksum="9DA95A5D45549282" modified="2020-08-12" version="1">MDDDQQFCLRWNNHQSTLISVFDTLLENGTLVDCTLAAEGKLLKAHKVVLSACSPYFATILSQQYDKHPIFILKDVKFQELRAMMDYMYRGEVNISQDQLAALLKAAESLQIKGLSDNRSSSSSGTAAAGGGVHQKQSDAPAAKTLQPPVPASKASGLTIENKRSLKSELLESDVSGSREGSTSPTSRKRKKVRRRSVDTNNLLIDNHDQHSNSSSHSMHTAGGANVAAAVAAAGAPAANVASSLSSASSTAAAGSNTSANVLSVAAVAAAAVLKKTETVQQAAAETLKLQKQQAAAAAAAAAAQSSDDEERCGPGSVATGTEHDDGNDLSDNEHDGDGGSSVKSGSRHHHLRDGSRRGKTSTSSMGEMMIEPKSEYEDVQDENVEDLTMDDEDLMDELDQAGPSHGGDVSKLWSDYYNFGSLTDDLYGRGSDASRQLNQARLMARKNGQNFPCLKCGKWYSTRSIMLRHMNHECGVEKKIQCKFCYKKFRRKWNLEQHIKRLHMSEKRKHHLHQQQQHHQQQQQQQQQQQQQQQQQQQQQQQASQQVSNRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VQJ5</accession>
    <name>A0A6E8VQJ5_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein rogdi</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the rogdi family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VQJ5"/>
    <dbReference type="EnsemblMetazoa" id="ACON006653-RA">
        <property type="protein sequence ID" value="ACON006653-PA"/>
        <property type="gene ID" value="ACON006653"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011528"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON006653"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037239"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043291">
        <property type="term" value="C:RAVE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028241">
        <property type="entry name" value="RAVE2/Rogdi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13618">
        <property type="entry name" value="LEUCINE ZIPPER CONTAINING TRANSCRIPTION FACTOR LZF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13618:SF1">
        <property type="entry name" value="PROTEIN ROGDI HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10259">
        <property type="entry name" value="Rogdi_lz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON006653-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="265" mass="30253" checksum="497280EAD6197869" modified="2020-08-12" version="1">MADCEKEEALNLQVEFEWVLHEEVHSVLKQLHVILVECAHRFPVPLYGNEGKKQDKFVLTAAPEQLKCIVTLTGDSITHADINFKVQRQQQQIQRTSITQDYPWKIQQVQDAANHLQQAINHIDNVDSAYHFKTSDEVLHILGNILGALQRGRTSLVVPRKKPIDELMKSRNMKALSPNLPEDLAISFYIQSHKLIFVAYQLTNFQGTMKFDSCQAECSVPWLNEVLVLFTVALQLCQQLKDKISVFSQYKDFTVGSRSPSALSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VSM9</accession>
    <name>A0A6E8VSM9_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ETS domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the ETS family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VSM9"/>
    <dbReference type="EnsemblMetazoa" id="ACON007327-RB">
        <property type="protein sequence ID" value="ACON007327-PB"/>
        <property type="gene ID" value="ACON007327"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_014114"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007327"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_043240"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040034">
        <property type="term" value="P:regulation of development, heterochronic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000411">
        <property type="entry name" value="Ecdysone-induced protein 74EF isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000418">
        <property type="entry name" value="Ets_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046328">
        <property type="entry name" value="ETS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11849:SF191">
        <property type="entry name" value="ECDYSONE-INDUCED PROTEIN 74EF ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11849">
        <property type="entry name" value="ETS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00178">
        <property type="entry name" value="Ets"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00454">
        <property type="entry name" value="ETSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00413">
        <property type="entry name" value="ETS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00345">
        <property type="entry name" value="ETS_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00346">
        <property type="entry name" value="ETS_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50061">
        <property type="entry name" value="ETS_DOMAIN_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ETS" evidence="9">
        <location>
            <begin position="618"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="205"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="485"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="30"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="220"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="280"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="324"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="365"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="508"/>
            <end position="517"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007327-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="714" mass="76135" checksum="8AF28BE77207D593" modified="2020-08-12" version="1">MPFIDDELLWSPDNDGRMVDLQACLQTESNQATASQTAELASELNTLEEQLENDSDELLRQLTENTFELEQFFQDFPATEIKVEENNNDLQLDEETNGQIFLQNCSQFLTSAAAAAQLSALSVTEPNDLDGGLQRLCSGGNDQLVGSGGLTTQQLQEQILLAQAQGLLQQNRFNNSLLAEKLLQNHHHHQQQQQQQLHLHHTHTNSQNGVTSLDALGVPSSSSSGGSNIGNNHHSSSNGSSSSNGGRANKPPDIVIKVDTEIPILPQSPSPPPQQRSSLAGGQSQQTASLLASTLQPSQQHRPLLHGLLSGTHLPQGPYHRGYSTSSTGSLPPSPADSGVSDVDSSSSGGQPACSDELKARLGIPTSSTGPVSNQPGQPSTRSRVNRSRCIRMAGPSATSLSSSSALSQVAAAAAAHLQSGPFLRPNFYHHSSPPLRNIWNQRSVPLSDNYHYLHSMNSPNGYPSAAAAAAAAAANHFASGGATNGTASNGPTGAAGNGTMGQHGHLAAPHHAGNPNGHHHGAHLGQQHGLGHHGHAGSGHVVGGGANSAAAAAAALMANGIGLGHHGQHGPHSVSSSCPDDLSYMLELGFQPRKLKKPKKPKLEMGVKRKSREGSTTYLWEFLLKLLQDREYCPRFIKWTNRDKGVFKLVDSKAVSKLWGMHKNKPDMNYETMGRALRYYYQRGILAKVDGQRLVYQFVDVPKDIVEIDCTGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VSN3</accession>
    <name>A0A6E8VSN3_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">eIF-4F 25 kDa subunit</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the eukaryotic initiation factor 4E family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VSN3"/>
    <dbReference type="EnsemblMetazoa" id="ACON007172-RA">
        <property type="protein sequence ID" value="ACON007172-PA"/>
        <property type="gene ID" value="ACON007172"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006684"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007172"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040388"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016281">
        <property type="term" value="C:eukaryotic translation initiation factor 4F complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000340">
        <property type="term" value="F:RNA 7-methylguanosine cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.760.10:FF:000046">
        <property type="entry name" value="AGAP007172-PB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.760.10">
        <property type="entry name" value="RNA Cap, Translation Initiation Factor Eif4e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023398">
        <property type="entry name" value="TIF_eIF4e-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001040">
        <property type="entry name" value="TIF_eIF_4E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019770">
        <property type="entry name" value="TIF_eIF_4E_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960:SF8">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01652">
        <property type="entry name" value="IF4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55418">
        <property type="entry name" value="eIF4e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00813">
        <property type="entry name" value="IF4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0810">Translation regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="11"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00032656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007172-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="220" mass="25606" checksum="F033FA3364CB485C" modified="2020-08-12" version="1">MAGKTNEETEQQQQQQNENETTEQSQELAEQSNAPESIDPECLIKHPLQHTWTLWYLEVDRTKKWTDSMNEVTSFSTVEDFWSLFNHIRGPSEIKVGGDYMLFKSHIRPMWEDDANKHGGRWTISMNKRLSDKCWLDTVLCLIGETFEHSDQICGATVNVRQKIDKISIWTADYDNRAAVLDIGRTYKERLGLRENIYYQMHKDTMVKLSSSGTKSTYTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VSP6</accession>
    <name>A0A6E8VSP6_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Sodium/potassium-transporting ATPase subunit beta-1-interacting protein</fullName>
            <shortName evidence="7">Na(+)/K(+)-transporting ATPase subunit beta-1-interacting protein</shortName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cell membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the NKAIN family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VSP6"/>
    <dbReference type="EnsemblMetazoa" id="ACON007012-RA">
        <property type="protein sequence ID" value="ACON007012-PA"/>
        <property type="gene ID" value="ACON007012"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005567"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007012"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_029582"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002028">
        <property type="term" value="P:regulation of sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008516">
        <property type="entry name" value="Na/K-Atpase_Interacting"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13084:SF6">
        <property type="entry name" value="SODIUM_POTASSIUM-TRANSPORTING ATPASE SUBUNIT BETA-1-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13084">
        <property type="entry name" value="T-CELL LYMPHOMA BREAKPOINT-ASSOCIATED TARGET 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05640">
        <property type="entry name" value="NKAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="30"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="61"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="206"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="505"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="239"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="271"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="289"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="512"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="538"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="564"/>
            <end position="574"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU368041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007012-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="652" mass="70585" checksum="E3980E22756FF487" modified="2020-08-12" version="1">MFLVSCFLLFSLSSPPQLFVIERQVFDFLGYMWAPILVNFFQILFVIFGFFGAYQYRPRYLVAYTVWSLLWLGWNIFLICFYLNIGILDRVSDSDLLNLGTGSVSWFEENGYGCRPTYPTNITSEDPYRPVRPERVDGCLLEYHIVEVVQSGIHCALAVFGIFGAICLGQTFLDEDDSLSRTSKKSKHRQSLYSIDFSRPVDAIRGAHPNDAYDDDDLMMGAGGGGGGAPSSLSPKPMTPRRVKRRSVMQRGTSGGGTVGRQSTSSRRGHSSSSAGSGAIASGSGGGAGHHHHHHHHHHRSSVRNSRRKTTHQNPVTKLLNQQQQHLQQQQHRLHHHGDALAGLEDAAGTMTLTKTFPPFVHNKNPDLTESNLKSLNNDYMLGQLNNFGQPVGAAGEAVVPGNNLNTFGAHHHHAAVQPPLPPHRRQVLVHTPTFLDGDTASSPIDPIYYNMNSSSPLLQQQTSWGVSTSSPQGMGAVPGSGIHAGLSNPVYQHSTTNLNHSPELNEEYYGTNGQNRPPSVRSSYSNFHGTRPLSSYLGGGGTGGVAQPGGITNRGYNVSPPGQHGGTTAGGGQVENVFANLTSGGGNTNHQQQYGGITGGSAGGTDPIAIPQLPHRRSVSRESIRAMQFLNSGPPAYNLNYHTPPDSETTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VSQ2</accession>
    <name>A0A6E8VSQ2_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">CRAL-TRIO domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_040226176.1">
        <property type="nucleotide sequence ID" value="XM_040370242.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VSQ2"/>
    <dbReference type="EnsemblMetazoa" id="ACON007358-RB">
        <property type="protein sequence ID" value="ACON007358-PB"/>
        <property type="gene ID" value="ACON007358"/>
    </dbReference>
    <dbReference type="GeneID" id="120951482"/>
    <dbReference type="KEGG" id="acoz:120951482"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_013040"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007358"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031528"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902936">
        <property type="term" value="F:phosphatidylinositol bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1200">
        <property type="entry name" value="Alpha-tocopherol transfer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.20">
        <property type="entry name" value="N-terminal domain of phosphatidylinositol transfer protein sec14p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011074">
        <property type="entry name" value="CRAL/TRIO_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10174">
        <property type="entry name" value="ALPHA-TOCOPHEROL TRANSFER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10174:SF166">
        <property type="entry name" value="LD40136P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03765">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00180">
        <property type="entry name" value="CRETINALDHBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM01100">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="1">
        <location>
            <begin position="106"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007358-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="324" mass="37548" checksum="317C7B6C3C10F8E1" modified="2020-08-12" version="1">MTNKIDLNKAPEAYAPYSFDLPDKFKIMAQDELREDDDMRENALKQFREWIAKHPLIRKCRTDAPFLLRFLRTKKFSIPAATEMLEKYLTIRQVYPHWFFKLDINDPDLEAIIDSGYLFALPERDEHGRKIIFSNAGKFDTSRFTSAQLIKIHSMVLEALQDEEESQISGYIHVTDDSELNIGFLSIWSFTDIRNLAHCVQNSLPMRQKENHFVSLPSFANKLSEFILSVLSEKLRNRVFVHRGWDEAKTKINPKLLPKEYGGTVPMAECIAQFKKQLKERRDIVLALDEMEIEINKSTIPWADSTDADIASGVIGSFRKLEVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VT14</accession>
    <name>A0A6E8VT14_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="4">SAM domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VT14"/>
    <dbReference type="EnsemblMetazoa" id="ACON007137-RB">
        <property type="protein sequence ID" value="ACON007137-PB"/>
        <property type="gene ID" value="ACON007137"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001949"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007137"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038657"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd09564">
        <property type="entry name" value="SAM_kazrin_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09567">
        <property type="entry name" value="SAM_kazrin_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09570">
        <property type="entry name" value="SAM_kazrin_repeat3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037614">
        <property type="entry name" value="Kazrin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059089">
        <property type="entry name" value="Kazrin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037613">
        <property type="entry name" value="Kazrin_SAM_rpt_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037615">
        <property type="entry name" value="Kazrin_SAM_rpt_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037616">
        <property type="entry name" value="Kazrin_SAM_rpt_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12776:SF1">
        <property type="entry name" value="KAZRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12776">
        <property type="entry name" value="KAZRIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25986">
        <property type="entry name" value="Kazrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07647">
        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAM" evidence="3">
        <location>
            <begin position="623"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="3">
        <location>
            <begin position="706"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="417"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="536"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="252"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="37"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="105"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="131"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="168"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007137-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="886" mass="96017" checksum="C7EF8E1EBD9FE4F7" modified="2020-08-12" version="1">MQLNGTAGTVTMAAASEPPEPPPRNPDKINASLKQLAESKTRSLDAADKAGASTTTNGGRPFRPILSLDNAKPSTKTFDIPERGTPTSAPATAFGTDHQPGGPEPGTSTSGNSSSSAAGTTQRQSNQPTLSGSSSSSSSGEAATANGTATARETAVPNASSASTTTSPQDKASGQAKQISPTLAASEPGGDNLFKNGVSDLESPQTTKIRLENERLSAEITRLKRLLENSPDGAVGGIDLLSQSNSFEVHAADDSSNRMEKLENELRISKELIQSLKLERKKLRADKNDLLSQVKQLCSSLQDKEQELRNFIRNYEQRIRESESNSAKQSTERERERWSLLKHARDEAERSIALAAQLNARDLQLQRTQEQLQEARRQLSGCMSDQESLVSAAPLTPPSGLMAHNAVAPASTPGVGYSNISGTPGDRGSCSADSGVRDFVAGSSDRESAAGDLNLSDGPCENGPCIQVDSDSVSLVSSHHNVYQYGTPKERSPTLSPLNAGAYSRSVDSAALSSSYRSVEQLGSPVDNENQTFARRLQQQQSASGAVPAASGKAQNTGLTGRTGRGGTWGSISRVFARSRNRNKNAPIDTEATDFQWSPLTEEGYAEKLRLLREASSMPMERWRASQVLAWLEVALGMPQYSGRCAENVKSGKVLLELSDAELETGLGTTHPMHRKKLRLAIEEQRRPELVRYPTIGQLGHTWVASEWLPDIGLPQYGESFMHSLVDARMLDTLSKKELEKYLGVTRKFHQASIVHGIHVLRIMKYDRQAIAMRRLQSENVDTDPIVWTNQRFIRWARSIDLGEYADNLKDSGVHGGLVVLEPSFSGDTMATALGIPPSKNIIRRHLATEFEALILPARYVIFCQMEEYPMDSARATSTLHKGSIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VT17</accession>
    <name>A0A6E8VT17_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">14-3-3 protein zeta</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the 14-3-3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040228716.1">
        <property type="nucleotide sequence ID" value="XM_040372782.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040228717.1">
        <property type="nucleotide sequence ID" value="XM_040372783.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040228718.1">
        <property type="nucleotide sequence ID" value="XM_040372784.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040228719.1">
        <property type="nucleotide sequence ID" value="XM_040372785.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_049463608.1">
        <property type="nucleotide sequence ID" value="XM_049607651.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VT17"/>
    <dbReference type="EnsemblMetazoa" id="ACON007643-RA">
        <property type="protein sequence ID" value="ACON007643-PA"/>
        <property type="gene ID" value="ACON007643"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON007643-RB">
        <property type="protein sequence ID" value="ACON007643-PB"/>
        <property type="gene ID" value="ACON007643"/>
    </dbReference>
    <dbReference type="GeneID" id="120953101"/>
    <dbReference type="KEGG" id="acoz:120953101"/>
    <dbReference type="CTD" id="36059"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000578"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007643"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039783"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.190.20:FF:000001">
        <property type="entry name" value="14-3-3 gamma 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.190.20">
        <property type="entry name" value="14-3-3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000308">
        <property type="entry name" value="14-3-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023409">
        <property type="entry name" value="14-3-3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036815">
        <property type="entry name" value="14-3-3_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023410">
        <property type="entry name" value="14-3-3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18860">
        <property type="entry name" value="14-3-3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00244">
        <property type="entry name" value="14-3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000868">
        <property type="entry name" value="14-3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00305">
        <property type="entry name" value="1433ZETA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00101">
        <property type="entry name" value="14_3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48445">
        <property type="entry name" value="14-3-3 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00796">
        <property type="entry name" value="1433_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00797">
        <property type="entry name" value="1433_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="14-3-3" evidence="5">
        <location>
            <begin position="6"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with phosphoserine on interacting protein" evidence="3">
        <location>
            <position position="59"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with phosphoserine on interacting protein" evidence="3">
        <location>
            <position position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00068216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000868-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007643-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="248" mass="28251" checksum="07024A653BC8B28A" modified="2020-08-12" version="1">MSTVDKEELVQKAKLAEQSERYDDMAQAMKSVTETGVELSNEERNLLSVAYKNVVGARRSSWRVISSIEQKTESSARKQQLAREYRERVEKELREICYEVLGLLDKFLIPKASNPESKVFYLKMKGDYYRYLAEVATGETRHTVVDDSQAAYQDAFEISKGKMQPTHPIRLGLALNFSVFYYEILNSPDKACQLAKQAFDDAIAELDTLNEDSYKDSTLIMQLLRDNLTLWTSDTQGDGDEPQEGGDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VUF8</accession>
    <name>A0A6E8VUF8_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Aa_trans domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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    <sequence length="456" mass="50401" checksum="3AFA62BEDFE4A1D5" modified="2020-08-12" version="1">MVNEMETFLPQDGKNGATVYKFTPAKSDVEANGTIQFDPFKERKLEHPTTDGETLTHLLKASLGTGILAMPVAFTYAGLAGGILATVFTAFICTHCAYVLVKCGHTLYRRTHRTAMSFSEIAEVAFENGPEWGRRWGMPTSYCIRYGLFITYFGTCAVYTVIIATNFQQVIEYYYGSPLNLRVMIALLLVPLILLSWIPNLKYLAPVSMVANVFMGVGLGITFYYLVTDMPPVNDRPLFLPVMQWPAFFAIVIFAMEAIGVVMPLENQMKTPQNFIGLCGVLNQGMAGVTLIYILLGFLGYVKYGDEAQGSITLNLPIEEIPAQAVKILIALAVYCTFGLQFYVCLDIGWVAIKDRFTKRPKLVEYVMRTILVTAAVLLAVAVPTIGPFIGLIGAFCFSILGLLIPIVIEMVTYWEEGFGPGNWIVWKNVVVFVFGIIALVFGSKSSIQDILALYA</sequence>
</entry>
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        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain>Mali-NIH</strain>
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</entry>
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                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
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                <person name="Galvin B.D."/>
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            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
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        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">Ngousso</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Lamellipodium</location>
        </subcellularLocation>
    </comment>
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        <property type="gene ID" value="ACON007877"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="Unconventional Myosin IB"/>
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    <feature type="domain" description="SH3" evidence="8">
        <location>
            <begin position="3"/>
            <end position="62"/>
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    </feature>
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        <location>
            <begin position="79"/>
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        </location>
    </feature>
    <feature type="domain" description="PH" evidence="9">
        <location>
            <begin position="275"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="395"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="624"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="410"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="436"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="446"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007877-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="673" mass="73840" checksum="B3EB7FA71BEF7F76" modified="2020-08-12" version="1">MDSSIITVQAQYSFKGSNNDELCFKKGDIITLTQREEGGWWEGTLGDKTGWFPSNYVKEYVGPLPLSETIRPPEEIQAFRSVVFRDLLESEKAHVAELRGLSENFLEPLEGSQILSSNEYTQLMCNFLEVVEMHEEFLQTLEDCNDRVGKVFLSNAPTMKKIHQFYCAAHPRAIVIVDKFRDELNVFMEKQGAAKPGLLVLTTGLSKPFRRLDKYAAILQELERHMESGHPDRGDTQRSIAVYKDIASSCSATRRQKELELQILTGPVRGWQGAELSTLGDIIHMGSVAVGPEHKDRYLVLFPQTLLILSVSQRMSAFKYEGKLPLTGITVNRLEDTDQLKNAFEISGTLIDRIVAVCQGPNEANRWVELLSPKSGTAEQGNTGAVDMKRNVSSSAVNIPQPPPHMSPPSATGNSHPSRSGTSSIVQHMSQHKRSQSFNHHLSYNQVQQQQQQQLQQQQLKQHQQNPNLSASPNSLNLNRLEANQAALNQKGFSEKANWNITNLRPAPPLRPAFLSSVAVSGTGSAGGGTGGGRSATASITGSIGGAAASCVGGGSSGGGSTILNTSDSVHHSLSFSSSSKVSPTYEEDALVLRVIEAYCAAYQSTSRNTMHSGVEYDEMTPTLRQLFISVRQLQQDMAQVKLQIAEERSQRGHLQQVLMGHLETYGAANTTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VV34</accession>
    <name>A0A6E8VV34_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">CD109 antigen</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">TEP1-F</fullName>
        </alternativeName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="24">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Binds covalently through a thioester bond to the pathogen surface resulting in pathogen clearance.</text>
    </comment>
    <comment type="function">
        <text evidence="14">Modulates negatively TGFB1 signaling in keratinocytes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">Heterodimer of a TEP1-N chain and an TEP1-C chain non-covalently linked. Forms a complex composed of TEP1-N and TEP1-C heterodimer, LRIM1 and APL1C; the interaction stabilizes TEP1-N and TEP1-C heterodimer, prevents its binding to tissues while circulating in the hemolymph and protects the thioester bond from hydrolysis. Mature TEP1 and to a lesser extent full-length TEP1 interact with SPCLIP1; the interaction is induced by microbial infection.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Heterodimer; disulfide-linked. Interacts with TGFB1 and TGFBR1. Forms a heteromeric complex with TGFBR1, TGFBR2 and TGFBR3 in a ligand-independent manner.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.</text>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON008364-RG">
        <property type="protein sequence ID" value="ACON008364-PG"/>
        <property type="gene ID" value="ACON008364"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000438"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008364"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038887"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004866">
        <property type="term" value="F:endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02897">
        <property type="entry name" value="A2M_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.20:FF:000001">
        <property type="entry name" value="CD109 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1930:FF:000001">
        <property type="entry name" value="CD109 isoform 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000155">
        <property type="entry name" value="complement C3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.130.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1930">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.690">
        <property type="entry name" value="Alpha-macroglobulin, receptor-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009048">
        <property type="entry name" value="A-macroglobulin_rcpt-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036595">
        <property type="entry name" value="A-macroglobulin_rcpt-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050473">
        <property type="entry name" value="A2M/Complement_sys"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011625">
        <property type="entry name" value="A2M_N_BRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041813">
        <property type="entry name" value="A2M_TED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047565">
        <property type="entry name" value="Alpha-macroglob_thiol-ester_cl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011626">
        <property type="entry name" value="Alpha-macroglobulin_TED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001599">
        <property type="entry name" value="Macroglobln_a2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019742">
        <property type="entry name" value="MacrogloblnA2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002890">
        <property type="entry name" value="MG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041555">
        <property type="entry name" value="MG3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040839">
        <property type="entry name" value="MG4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11412:SF136">
        <property type="entry name" value="CD109 ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11412">
        <property type="entry name" value="MACROGLOBULIN / COMPLEMENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00207">
        <property type="entry name" value="A2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07703">
        <property type="entry name" value="A2M_BRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07677">
        <property type="entry name" value="A2M_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01835">
        <property type="entry name" value="MG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17791">
        <property type="entry name" value="MG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17789">
        <property type="entry name" value="MG4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07678">
        <property type="entry name" value="TED_complement"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01360">
        <property type="entry name" value="A2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01359">
        <property type="entry name" value="A2M_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01361">
        <property type="entry name" value="A2M_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01419">
        <property type="entry name" value="Thiol-ester_cl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49410">
        <property type="entry name" value="Alpha-macroglobulin receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00477">
        <property type="entry name" value="ALPHA_2_MACROGLOBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8" id="KW-0391">Immunity</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0882">Thioester bond</keyword>
    <feature type="domain" description="Alpha-2-macroglobulin bait region" evidence="21">
        <location>
            <begin position="457"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-2-macroglobulin" evidence="22">
        <location>
            <begin position="705"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-macroglobulin receptor-binding" evidence="23">
        <location>
            <begin position="1324"/>
            <end position="1413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1435"/>
            <end position="1457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="1443"/>
            <end position="1457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00056820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00057615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00063008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00063781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00069665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00078071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="SMART" id="SM01359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="SMART" id="SM01360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="SMART" id="SM01361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008364-PG"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1481" mass="164284" checksum="524B68ACFD5292A1" modified="2020-08-12" version="1">MRRSTMFASKKDPAIRILSTSSSSLLAVCLVLSVVLVPAVQCEGHYSIVGAKLLRPNSEYHVAVTNQDVSEPIRFSLAITDASSVIAKQEITLNTGETRLVPFAIGDIPESSYKLVAEGLSGLTFKNETDLEYQQKSFSVFVQTDKSIYKPGDTVRFRVLVLDPNTKPLQKADNISVHINDAKANRIKQWKEGKLVKGVFESELTLSTAPVLGAWTINVEVLGSKHNKVFEVDEYVLPKFEVTVESPGITTFKDGKVKAIIRSKYTYGKPVKGEATVSVSPEFQFNYVQPFAKDVITRKVIPIDGKGSVEFDLREDIHLEGDYSRNIVIEAVVEEELTGRKQNASAKVMIYDRRYKMELVKSDDNFKPGLPYTAWLKVSYQDGAPVQDQTNPVEVKQSSFESTTSVQNYTLDQNGMAKLEINTEVNSSYINVVGVYLGQEFYLHGISKAESDVDSYIRAQVLTEMPLVGKDVLVEVTSTSPMKYFTYQLLGRGDVLLSNTIAVPESKTQSFKFPATFAMVPRAKLVVYYIASNGDMVSDSKVITFDSELQNFMKVSLSKEQSKPGQDVEISISTNPDSYVGLLGVDQSVLLLKSGNDITKQQVFSELEKYEERSYGFYRRKKRFAWNPHAEHRDFSTVGAFVMSNANDPPRPFVLYFHRAGLPGMMMPMAASASVVTRVAPVAKFGAGAFAPVQSAPVRKEFPETWLWQTIAPASFSGEKTLQKKVPDTITSWIITGFSVNPIYGLGLTQQPRKLNVFLPFFVSTNLPYSVKRGEVVAIPIVVFNYMEDDQTAEVVLHNDEQEFEFADVENEVVESNKVELFRQKRLDIASNTGKSVSFMVKPKKLGHITIKVTAKTKIAGDAVERQLLVEPEGLPQFINKAAFIDLRAAPELTKTFEVEIPKNAVPDSTRIEVAVIGDVMGSTIQNLDSLIRMPYGCGEQNMLNFVPNIVVLDYLKATNKLTANIEAKAKKFMEAGYQRELGYKHRDGSFSAFGENDKSGSTWLTAFVARSFKQAANHITIDEGVIDKSLEWLSDHQAPNGSFPEVGVVSHKDMQGGSGSGVALTAYTLIAFLENINLVDKYKNTINKAIDYVYRNTESLDDTYALALAAYALQLADHSSKGLILSKLDTKATTDSDSKWWHKPIPETEQKNPWYSRPNSVNVEMSAYGMLAFLEAGLDTDALPIMKWLIGQRNDKGGFQSTQDTVVGLQALAKLAAKITSPNNDVTLTAKINENQEKRMTVNAENGMILQKFELPSAARNIEIQATGSGFAVVQLSYKYNMNVTGEWPRFVLDPQVNANTNPDYLHLSVCASFVPSAGQNVSNMAVMEVGFPSGFTADSDTLPSLENMPFIKKVETKDGDTTVVLYFDSLDQRELCPTISAFRTHKVAKQKPAPVVIYDYYDNSRIARQFYDGPKASLCDICENEDCGEACSIRSQKQRSSDSPSRQPTVEGTMQSGSQTVSVSFFTFLLATLLVRMFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VV54</accession>
    <name>A0A6E8VV54_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VV54"/>
    <dbReference type="EnsemblMetazoa" id="ACON008409-RA">
        <property type="protein sequence ID" value="ACON008409-PA"/>
        <property type="gene ID" value="ACON008409"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008409"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008409-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="113" mass="12578" checksum="D95A5B99B42AD48D" modified="2020-08-12" version="1">APLTRVQLVRVWPVLFPNPLISSAGALFSGRKQYETHAHTYTQLTTNTHAYPAAAARRHSGRILELFVCVCVCLCVCASVPVSVVLARRRRRVCKARLVAVIYKSEVCILVMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VV59</accession>
    <name>A0A6E8VV59_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="5">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Perinuclear region</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8VV59"/>
    <dbReference type="EnsemblMetazoa" id="ACON008412-RC">
        <property type="protein sequence ID" value="ACON008412-PC"/>
        <property type="gene ID" value="ACON008412"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004485"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008412"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037203"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02670">
        <property type="entry name" value="Peptidase_C19N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000009">
        <property type="entry name" value="Putative ubiquitin carboxyl-terminal hydrolase CYLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.190">
        <property type="entry name" value="CAP Gly-rich-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036859">
        <property type="entry name" value="CAP-Gly_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000938">
        <property type="entry name" value="CAP-Gly_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11830">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01302">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01052">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74924">
        <property type="entry name" value="Cap-Gly domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="USP" evidence="15">
        <location>
            <begin position="250"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="19"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008412-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="613" mass="68386" checksum="3E305442B63B94F4" modified="2020-08-12" version="1">MLPDTYDKDSFDGIPLTVLEHFDWEDEPTEATERPLASDAEEELNDGAAITANGSIGPADGTSHPTANGLVPRIGSISPESDSGGPAASPLQELPNDPSLGVGSMVEVTLDGGAFEAPGAATAPPATMTDPLLYGVIRWIGPLPTGGGNHRKVMVGVELEDEPIDPTLETTNGTHNGVRLFRCPANRAIFVHTSQCSRDRRFQDIPPMSPGSKTDTNMFGKVDCPVVKGRVPPLKILKLEELEEICGKFKGIQGHHNSCYLDATLFAMFTFTSVFDSLLFRPKEPEDNPQYEEVQRVLLEEIVNPLRKNHFVRADRVLKLRQLLDRLSSVTGLMSEEKDPEEFLNSLLAQILRADPFLKLSSGLDTFYYQLFVEKDERLNLPSVQQLFEQSFLASNIKLKEVPSCLIIQMPRFGKNFKMYPRILPSQVLDVTDIIEDSPRQCWVCGKLAEYECRECFGKMQCEGLEGTAICKSCIDSVHHHSKRLNHKPVPLSVPQDFIPMAPHCEVPRLYMELFAVVCIETSHYVAFVKAASGQDAPWCFFDSMADRNGEQNGYNIPKMVPVPDLPRWLTEEGSRALNEEAVNDKMLPEHAKRLLCDAYMCMYQSTDVMMYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VWJ7</accession>
    <name>A0A6E8VWJ7_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nuclear transcription factor Y subunit beta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">CAAT box DNA-binding protein subunit B</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Nuclear transcription factor Y subunit B</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes. NF-Y can function as both an activator and a repressor, depending on its interacting cofactors.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NFYB/HAP3 subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040238016.1">
        <property type="nucleotide sequence ID" value="XM_040382082.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VWJ7"/>
    <dbReference type="EnsemblMetazoa" id="ACON008344-RA">
        <property type="protein sequence ID" value="ACON008344-PA"/>
        <property type="gene ID" value="ACON008344"/>
    </dbReference>
    <dbReference type="GeneID" id="120958980"/>
    <dbReference type="CTD" id="35261"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001552"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008344"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_029425"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
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        <source>
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        <source>
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        </source>
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    <sequence length="168" mass="19151" checksum="0D8A7A59BB1B3841" modified="2020-08-12" version="1">MQDDMDGTHSEDSSCEQLLKAGTPLREQDRFLPIANITKIMKKSVPNNGKIAKEARECIQECVSEFISFITSEASDRCHMEKRKTINGEDILCAMYALGFDNYIEPLKLYLSKYKENIMSERQASEERGEPVQTGESSRQGNVIGNAEIIYQSEDGTLYFKNEPYEQM</sequence>
</entry>
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    <accession>A0A6E8VWX7</accession>
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        <name type="scientific">Anopheles coluzzii</name>
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        <dbReference type="NCBI Taxonomy" id="1518534"/>
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            <taxon>Anopheles</taxon>
        </lineage>
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    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
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                <consortium name="EnsemblMetazoa"/>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="6">Ngousso</strain>
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    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="286" mass="31819" checksum="BE4EC6222AE76D32" modified="2020-08-12" version="1">MDCKRILRCSALVVLVLVTLVNAQSSDVLLPNYDQASTSLKIFDIAKALTLGCNITQEGNFELSWAKDGKNVSEVESLKDRFKILAAERKFVISRALETDAGQYTCSVPQLGVSKSFNVVANVVVKFESTEIGKTNIVEGERLTLHCIAYGTDPKITWTVGNNTYNASTDHIVLEEDERGVENAKLIIDSIKLDDYADYTCEARNNATEFTGKPAQVLITVRVRGKYAALYVFLGIIVEVVLLCAIILICEKRRNKTEIEESDTDQSPDQNKNGYHGNESELRQRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VWY8</accession>
    <name>A0A6E8VWY8_ANOCL</name>
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        <submittedName>
            <fullName evidence="15">SUN domain-containing protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">Ngousso</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="10">Interacts with EMP65.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endomembrane system</location>
            <topology evidence="8">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the SLP1 family.</text>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON008473-RB">
        <property type="protein sequence ID" value="ACON008473-PB"/>
        <property type="gene ID" value="ACON008473"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009925"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008473"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030764"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034975">
        <property type="term" value="P:protein folding in endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000099">
        <property type="entry name" value="Uncharacterized protein, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045120">
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    </dbReference>
    <dbReference type="InterPro" id="IPR012919">
        <property type="entry name" value="SUN_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12953">
        <property type="entry name" value="MEMBRANE PROTEIN CH1 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12953:SF0">
        <property type="entry name" value="SUN DOMAIN-CONTAINING OSSIFICATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07738">
        <property type="entry name" value="Sad1_UNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51469">
        <property type="entry name" value="SUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="45"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="SUN" evidence="14">
        <location>
            <begin position="245"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="168"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="473"/>
            <end position="497"/>
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            <end position="545"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
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            <begin position="686"/>
            <end position="719"/>
        </location>
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            <begin position="732"/>
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        </location>
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    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="896"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="184"/>
            <end position="204"/>
        </location>
    </feature>
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        <location>
            <begin position="787"/>
            <end position="804"/>
        </location>
    </feature>
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        <location>
            <begin position="836"/>
            <end position="847"/>
        </location>
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        <location>
            <begin position="1111"/>
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    </feature>
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        <location>
            <begin position="1365"/>
            <end position="1391"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00046288"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00061226"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00064635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008473-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1454" mass="155924" checksum="B78C89926299E530" modified="2020-08-12" version="1">MLHLPTVDLYMVVNLYLVAFCYIKWQLSMWLVGAITERAGRMRKLTVLVTFCWVALITQIVAISNLYHDGLQDIPGPPSIITLVDNQHHELNNIESKLEENLEATLKNLSHNNVHTVQTTVPLHGDDHGQLAAEEEAHANGTTVGDQQELSIGDGQENEQPLLVMIPAKDPTEGPASSSTPSGEPAATQETTAEATLTDATAPTVLPPTKQLEVNLTEENPMPVFSEWAQKQMAEAEKKLGEVVNASAMKKGTKPAGSKAGTGSMKLRAKNYAAPECGAKIIASNPEAQSTGSVLTAPKDEYLLNPCTSKIWFVVELCEPVQAERIELANFELFSSSPKEFSVSVSNRFPTRDWANVGQFTAKDERDVQSFLLHPHLFGKFVRVEILSHYNQEHFCPVSLFRVYGTSEFEAFETDNTPLQPDEDDDDDELVAVLGGVGVGKDPVTDKSKPVAGGEGTLADKRFVINGGVAQRDGQTVEPQQGVDATTGAPKGGKNPNNILKSAGEVVMNMVKKAAEALGKSGNESNDAATPGSHPSPGGKIPLTDVLTGLPSPASPSCVTLAYTIRCVNCTDHFRARLESLMNCKHNLLTSLLGVARIEHAFDSAQHFLCANVLGFNLPRSLQGESEGAGELVTVYPSVCLNMRYSVLNLLPDELVAGLCNTVAFDLKLLAKVEASDAEGREIDGSVVAGFDGPSQNALDLPQGENTKQGEHHQQHQVEQTLDVETTAQNGNEKGEQQEQNAVQEDSEKGSPPADAAIDPSGSENASKEDVNMFATEPTPPAATPAEPERDEASQNVDGQKEQEQQQQQEDFANNQHWETIDDQSEMAGTGPSGSGAFTTGPAATTVTPGMATGQQKVQPESVFLRLSNRIKALERNMSLSGQYLEELSRRYRKQVEELQHSYAKTLHEIQEQNQRMADSETKLREVNERLRQELVDFQATATDWRNIALAVLTMIVLMLFTLLAMVRSVARSVNRLTAGHPLEGQHQQQQHPVPLPKSVIDRELAQVGSDAKPIRGRMLRRKSIDGMPTSGGSVDLTGSTVSSASGIIIATAEPSTVATSLSPGRLRKKRPSEEALNISGTYVNLLIDDDVREVGVPPQSSVPPLAMERKKSKQKHRKVSAPSMMGVISSSTPLATGQGVAFSSHEPAKRSLSMIEARQMVNGGGQQTPEKDTSSRIDELPYLEDNDEFIIPTASDLSYDEYMPGSNASTAEAGLTPNEMEMRKMNGTANTAATTMTTLSDRNGSSGMSPSGEDSLDGGVQQKAATKSSRRLSSPAFFKSSLLRSSLGSKKHRSAKKASAANGSGGSSSSGGSSANTSSNSSNSSNTSSALRELGAGDAGMSESGRGNAQDSSWYRWWSSEQSKGVLSSPSPASSQEVAASPEGKQQQRQRIGKAKSASPSDGASSKLLLLGESPVKLSVSFNGDLPANGGSGGVLERKSSRGSIRRLFRKVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VX97</accession>
    <name>A0A6E8VX97_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NodB homology domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VX97"/>
    <dbReference type="EnsemblMetazoa" id="ACON008512-RB">
        <property type="protein sequence ID" value="ACON008512-PB"/>
        <property type="gene ID" value="ACON008512"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007454"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008512"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034662"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10975">
        <property type="entry name" value="CE4_CDA_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.370:FF:000003">
        <property type="entry name" value="CLUMA_CG003232, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.370">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052740">
        <property type="entry name" value="CE4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002509">
        <property type="entry name" value="NODB_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45985">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45985:SF12">
        <property type="entry name" value="CHITIN DEACETYLASE-LIKE 5, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01522">
        <property type="entry name" value="Polysacc_deac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NodB homology" evidence="2">
        <location>
            <begin position="437"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="181"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="321"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="19"/>
            <end position="30"/>
        </location>
    </feature>
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        <location>
            <begin position="47"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="76"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="103"/>
        </location>
    </feature>
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        <location>
            <begin position="106"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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            <begin position="120"/>
            <end position="130"/>
        </location>
    </feature>
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            <begin position="208"/>
            <end position="226"/>
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    </feature>
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            <begin position="227"/>
            <end position="237"/>
        </location>
    </feature>
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            <begin position="322"/>
            <end position="331"/>
        </location>
    </feature>
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            <begin position="332"/>
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        </location>
    </feature>
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        <location>
            <begin position="346"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008512-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="772" mass="86824" checksum="6016733F6C387C6D" modified="2020-08-12" version="1">MYSHELQTCDWPRNVGCDLPALSAPSAPASRTVTPRVPQVLSRVRFSSSAPSAPSGPSGPSGPSGPSGPSGPSGPSGPAEPSAPARIQQIRNLPPPPPPPANPNPVITTRGQPKNSPQEDIAKLYAEAHETLPPVEEDESDRQQRVYRGQPSTVSQVQRDRDGILQQQSVNAIPTQAKVGSFAYSAQPQPFRVETSSPAPPSRYVPEQQQSQQQQQQQQKTQLTQSNRNYYNTSNYEPSAYDSYYAVYDEDVELYRDVEYQPQPQKPVVSSTYRPTVVTTVAPTSQRDPYVTSPRTTYQRPSYQADYDDALVAQLEEDRYNQKQTNTQIRVSNNNSNNNRNRGSSQYATDGQSTVSANRGTHPPRARPTLKPSQNIVSKAQEFIDIYRYPPTRPEPLYPQPTPDKTAAKCRKDVCLLPDCSCGGKDVPGELPVEQVPQIVLLTFDDSVNDLNKQLYQDLFERGRVNPNGCPITATFYVSHEWTDYSQVQNLYADGHEMASHTVSHSFGESFSPKKWAREVAGQREILSAYGGVKLEDVRGMRAPFLSIGGNKMFKMLHDFNFTYDSSMPVYENRPPSWPYTLDYKIFHDCMIPPCPTKSYPGVWEVPMVMWQDLNGGRCSMGDACSNPPEAEGVYKMIMKNFERHYTTNRAPFGLYYHAAWFTQPHHKEGFIQFLDAINSMKDVFIITNWQALQWVRDPTPLSRINSFTPFQCNYAGRPKRCNNPKVCNLWHKSGVRYMRTCQPCPDIYPWTGKTGIRSSRIDNDIEVADTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VYZ5</accession>
    <name>A0A6E8VYZ5_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ABC transporter domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VYZ5"/>
    <dbReference type="EnsemblMetazoa" id="ACON009466-RD">
        <property type="protein sequence ID" value="ACON009466-PD"/>
        <property type="gene ID" value="ACON009466"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON009466-RE">
        <property type="protein sequence ID" value="ACON009466-PE"/>
        <property type="gene ID" value="ACON009466"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011440"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON009466"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034839"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050352">
        <property type="entry name" value="ABCG_transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041:SF133">
        <property type="entry name" value="GH24286P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
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    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="171"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="201"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="241"/>
            <end position="268"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
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            <end position="303"/>
        </location>
    </feature>
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            <begin position="309"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="339"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="390"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="4"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="ABC-2 type transporter transmembrane" evidence="9">
        <location>
            <begin position="147"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00005814"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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        <source>
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            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00005"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF01061"/>
        </source>
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        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009466-PD"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="420" mass="48031" checksum="BD4EE6E0B5B5B5F8" modified="2020-08-12" version="1">RDILKDISGEFRAGELTAIMGPSGAGKSTLLDILAGFLEIATHDYGRLNDSLVEKMMNGQSNFYRNPEPFSPTFESKLLPIKESSPDGPMVLLEMPLFPEHDNGDARNQQRPVSPVLATPKKLAKKLSFNPERWCGRDEIYTTSFCRQFSLLLLRTFLILSRDRSLMTMRFAIHCLIAPLIGMLYFGIGNQAAQIFNNYNYVFFSIMFLMFTAFSSMTMAFPLELPIITREHFNRWYSLRAYYIAMTVADIPIQLLCTVTYILITYYMTGQPPEAFRVGLFTLICLMVAWVAQGLGLLVASLFDVKNGAIFGPFFICPFLIFSGFFIHLTDAHPIMHWLFHISFLKYALQGAALAIFGYDRPRMDCDETYCHFVLPKKFLKEVDMLEADFVEAVIALGVIFFVFRVSAFYVMQYRMKNKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W0F2</accession>
    <name>A0A6E8W0F2_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="7">SERPIN domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the serpin family.</text>
    </comment>
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    <dbReference type="EnsemblMetazoa" id="ACON009670-RB">
        <property type="protein sequence ID" value="ACON009670-PB"/>
        <property type="gene ID" value="ACON009670"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007514"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON009670"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033089"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:negative regulation of proteolysis"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="serpin28D-like_insects"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Antithrombin, subunit I, domain 2"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461:SF342">
        <property type="entry name" value="SERINE PROTEASE INHIBITOR 28DC"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0722">Serine protease inhibitor</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
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            <begin position="1"/>
            <end position="25"/>
        </location>
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            <end position="623"/>
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            <end position="181"/>
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            <end position="164"/>
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            <end position="175"/>
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        </source>
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        </source>
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        <source>
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        </source>
    </evidence>
    <sequence length="623" mass="68877" checksum="21576E4E5C895DFE" modified="2020-08-12" version="1">MRSHYSAGWTLALVLLVSSLLAVSAANNRGQPAKPAAADQQANRLHAPTANTKKVSDSVTNLAAKIANALSNQKSKTEIFSPVSIAGALSLLLLGSGGQTQQELLAVMGLNTGQLSFQDIHLSFGRLFQDLVSNDPSLEPLVTWRLNDKCNRYNEDEEDEEDDFINSQSPSNDQQPTKRERKAHEITLANGIFVQRNIPLSDTYRNQSMTYYSSEVQSLDFELDTSGSTRLINRWVSDKTHGKIPNILPSALPASTTMVLASALYFKALWAESFIDGATKPREFYPDGKGQPPVMVDMMAHGGCFPYYESVELDARIIGIPYKHNVSTMYVIMPNNSNRAKLQQLIPKLTSEVVKDLIENMAIKTSIILFPKMHISNTMDLKRVLQQLGVSSLFQAERSNLSPMLNEESQPDTFINRVQAPTTPAKWRPTVNIADYENRPTSTRPGNNLNDVLIFSRFQNETMEQTTVVPEAADTTEPLTIETTSQSFVPLTNRPSGRVGRTKRDVSYKVPSSKQAAQAVGPLSSKDFILHKRIVKENGPVGKKGLRRRSKRAAQQLYVSNAVHQVDLEVNETGTEGGAATIVTLNRSGTSVVFRAEAPFLLLIRNDQTNLPLFYGPVYHPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W0F8</accession>
    <name>A0A6E8W0F8_ANOCL</name>
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        <recommendedName>
            <fullName evidence="7">Chitin-binding type-2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8W0F8"/>
    <dbReference type="EnsemblMetazoa" id="ACON010466-RA">
        <property type="protein sequence ID" value="ACON010466-PA"/>
        <property type="gene ID" value="ACON010466"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009694"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON010466"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042389"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.140.10">
        <property type="entry name" value="Chitin binding domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002557">
        <property type="entry name" value="Chitin-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036508">
        <property type="entry name" value="Chitin-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051940">
        <property type="entry name" value="Chitin_bind-dev_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23301">
        <property type="entry name" value="CHITIN BINDING PERITROPHIN-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23301:SF0">
        <property type="entry name" value="CHITIN-BINDING TYPE-2 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01607">
        <property type="entry name" value="CBM_14"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00494">
        <property type="entry name" value="ChtBD2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57625">
        <property type="entry name" value="Invertebrate chitin-binding proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50940">
        <property type="entry name" value="CHIT_BIND_II"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0147">Chitin-binding</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026172170" description="Chitin-binding type-2 domain-containing protein" evidence="6">
        <location>
            <begin position="19"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="7">
        <location>
            <begin position="20"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="7">
        <location>
            <begin position="78"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="7">
        <location>
            <begin position="129"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010466-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="194" mass="20714" checksum="3BF8397AFA278E35" modified="2020-08-12" version="1">MIRSVLALFLLGCATAQAQTNPCQGQPNYRFVADATSCYKYFTCVNGEPFPQECPVPFVFVEATQSCDYGDRNACVNCPATGIQNFPVSGSCTQFIQCIEGSQFPRECPPGTAFDSNSGQCNLASAVNCIACPAEDDPANPTFIPDATDCRKYFICVGGSGIEQICPEGTSFNPSLNVCDLPDRVQCPAVVSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W0G8</accession>
    <name>A0A6E8W0G8_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DH domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8W0G8"/>
    <dbReference type="EnsemblMetazoa" id="ACON010473-RC">
        <property type="protein sequence ID" value="ACON010473-PC"/>
        <property type="gene ID" value="ACON010473"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004053"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON010473"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037586"/>
    <dbReference type="Proteomes" id="UP001105220">
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        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="term" value="P:endothelial cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007266">
        <property type="term" value="P:Rho protein signal transduction"/>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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        <property type="entry name" value="PLECKSTRIN HOMOLOGY DOMAIN-CONTAINING FAMILY G MEMBER 5"/>
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    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
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            <end position="71"/>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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            <end position="305"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="326"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="630"/>
            <end position="685"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1086"/>
            <end position="1106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="63"/>
        </location>
    </feature>
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            <begin position="269"/>
            <end position="282"/>
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            <begin position="330"/>
            <end position="344"/>
        </location>
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        <location>
            <begin position="635"/>
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            <end position="1106"/>
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        </source>
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            <dbReference type="PROSITE" id="PS50010"/>
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            <dbReference type="EnsemblMetazoa" id="ACON010473-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1158" mass="126973" checksum="5692E4A5E78F4167" modified="2020-08-12" version="1">MDENVQRSNESIKQRNLVHSIEQISPIRDVYSILPESNVHSTGQSQTLHTQPSASSSPTHISNTSPSAATSLSTLLSPTKISQDVNLQSTSKNGAHQETFSNSLATATPAGFSLILPQNLNRSFDQTAAQSPPKYQIYHQFSRISSTTQQFIQRQQQQQLQQQEQHQQIFHPQAQPLQLQQSPQTQPASPVTNYQTPLFRKASYGSSHYAHYSNVDPLTVNAGTLSARPNNTYHQYQTTHQCSKTVDQRSNNPFILNFNNISGCCLKNSPTSSSSPPATSPTDRSITSAPITGNPQSVTSGSSSSAITAVVSSGTKRTRSLLLKSTSPLQQTTHSSPSHTVSSPQGPPVPPRLSHIHAIGSVSPANTGPTFSNHPSIDPISTTTSSNASSEAIVMSSIVTGTCYVTPPTPAPLITSDKHRELPSMTFGSVTPTVVSNTLPDSTTLTASGHNKTPTDGNESGLLTKEMIDKSVRKKRDDFLRTTMKICLVVSPPSKLQMKSLSLTHLDEIERQATTPTIITNDPHVRKVSSSEYFSVCFESDQNDPGVLIPATKGVRLAECLRASLSRRSLSFSQIYVTDNGFNNPHETGLRYTEPLDANMDISALAGRTLIVSERDGAPNRRTGGHLTLQKAASVGNQPSIMSSNSKLFTSTSTDDNYDQSPKASSASGKQSKQKWTIPFGSKGPQKSKLCELLDSYNKEIPKSTSSSSNFNNPEYVDALVGLRNLPQCWTDIVNCAGMSEAETKIQSAIWELVTTEVYYILALQTVTDLFLACLEDIQSQNILTDVDQNKLFSNIRDIWEANLRFWTLYLHPMVKHSQRTKDPMSIYYFQRGFVDFATIFTPYTKYCAEQSTCQFYCKEMYHNNALFMTYCAWCESQKMCNRLRLADILVRPMQRLTKYGLLLAAIKKHINDEAEGEAIDAMIHSVEEFVAGVNSHLTTRQESERLKGINARIDCYEVVDSNNETLDRLVKQHSIMFDLCQPMRGIDHGRKVFVEGDLKYKDGTGKNTLSVPGTSNSQSHSGMVLLSPSQRGISYPPPSPTRATLRRGFAFSTSIKNPPLIKTRNVSSQQSFALSQQQSFNSASLAQHQQCLPSPSNTLHGAPNNSGTNSAIEGCAVASGLTLSSANVSFFKRKNSFQSVPNNSATSSVDQSLPNET</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W0H1</accession>
    <name>A0A6E8W0H1_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="10">FIP-RBD domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
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            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cleavage furrow</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Midbody</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Recycling endosome membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8W0H1"/>
    <dbReference type="EnsemblMetazoa" id="ACON010445-RA">
        <property type="protein sequence ID" value="ACON010445-PA"/>
        <property type="gene ID" value="ACON010445"/>
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    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002532"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON010445"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037687"/>
    <dbReference type="Proteomes" id="UP001105220">
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    </dbReference>
    <dbReference type="GO" id="GO:0032154">
        <property type="term" value="C:cleavage furrow"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:regulation of cytokinesis"/>
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        <property type="entry name" value="Nuclear fallout, isoform D"/>
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        <property type="entry name" value="Rab11-FIP3/4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051977">
        <property type="entry name" value="Rab11-interacting_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15726:SF7">
        <property type="entry name" value="NUCLEAR FALLOUT, ISOFORM J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15726">
        <property type="entry name" value="RAB11-FAMILY INTERACTING PROTEIN"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF25450">
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    </dbReference>
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        <property type="entry name" value="Eferin C-derminal domain-like"/>
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            <end position="306"/>
        </location>
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            <end position="241"/>
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        <source>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="PROSITE" id="PS51511"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010445-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="308" mass="36118" checksum="35D480F7F3B5BC87" modified="2020-08-12" version="1">MRQNLNVPHYLTENRLNATVTTTYQSQKLVLEILQRQVTNLADTQSNVDDRTSRTKTEYAVLQARYHMLEEQLRETELRAEERLAEEQKRHRELLARVEREAKLQNENCQIRIRTMEMEVTSLREEIQRLRLQSDKQAADLHATEEKLEKARDSLMISQQDLAEARAEEKKHRAEKQAAEELMVELGKECERLRSERGPALPTTSPESLRLEELHQEMDELRQKNKSLEEANEELQAMMLTRSIEEGRNLLNGTSNSLAQELEAMSQNQLQVAFQEKEEENRRLKHYIDTMLLNVVENYPQLLEVKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W2J9</accession>
    <name>A0A6E8W2J9_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Presynapse</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8W2J9"/>
    <dbReference type="EnsemblMetazoa" id="ACON010286-RB">
        <property type="protein sequence ID" value="ACON010286-PB"/>
        <property type="gene ID" value="ACON010286"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009032"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON010286"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034840"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048788">
        <property type="term" value="C:cytoskeleton of presynaptic active zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098882">
        <property type="term" value="F:structural constituent of presynaptic active zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007274">
        <property type="term" value="P:neuromuscular synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048167">
        <property type="term" value="P:regulation of synaptic plasticity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019323">
        <property type="entry name" value="ELKS/CAST"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18861:SF0">
        <property type="entry name" value="BRUCHPILOT, ISOFORM J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18861">
        <property type="entry name" value="ELKS/RAB6-INTERACTING/CAST PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10174">
        <property type="entry name" value="Cast"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0770">Synapse</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="99"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="163"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010286-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="655" mass="73968" checksum="682CC204C9B970D5" modified="2020-08-12" version="1">MGSPYYRDMDEPISPAGGHHRSRSATRAPTHSLEYPREQLRTRYQSLDRGLVDPHDREFIPIREPRDRSRDRSLERGLYLEEELYGRPPRQAMPERGYLGDLQVQNGELQRELGNLKKELELTNQKLGSSMHSIKTFWSPELKKERALRKEESAKYSLINDQLKLLNSENQKQAMLVRQLEEELRLRMRAPNIEMQQQMEALYAENEHLQREISILRDTIKELELRIETQKQTLQARDESIKKLLEMLQSKGMGKEEERQMFQQMQALAQKQVSMFDITNCTTTTADLMRTSFYPAQPTSVYNSNYHNIAPASKLLDVRSGRLPARRYSISGSVPLSSMVDYCNLSENAANLYTQSANLTNLLNETTKSLSRSSNILNMRSLGTTASGDSGSNLPMCHATTHFTQSLLASAPTSSSTTICGVLDPSPSLPFERMGASTATSAGTTTGLHCSYPNTSSISHLPNAVASNSNLSYYNHHLQHHLPHVNQQNSIPVPQQLQFSQHSSFCNTGCRPSTDYLLSKPIYSNNTINAASNATSFLSHPHSVLSHTKNLLSQHQFLSNPAITRSLTNLPSSTTSSYPYERPISSVTALRYPSSFDNTNLLHTMHDNIDCNAMLRYPTSYHAPYSKVDLDYSRASDMKRQVSFKFDVDTLSIDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W2L6</accession>
    <name>A0A6E8W2L6_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cuticle protein 6</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8W2L6"/>
    <dbReference type="EnsemblMetazoa" id="ACON010845-RB">
        <property type="protein sequence ID" value="ACON010845-PB"/>
        <property type="gene ID" value="ACON010845"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_008847"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON010845"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035827"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062129">
        <property type="term" value="C:chitin-based extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008010">
        <property type="term" value="F:structural constituent of chitin-based larval cuticle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031311">
        <property type="entry name" value="CHIT_BIND_RR_consensus"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050468">
        <property type="entry name" value="Cuticle_Struct_Prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000618">
        <property type="entry name" value="Insect_cuticle"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10380">
        <property type="entry name" value="CUTICLE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10380:SF224">
        <property type="entry name" value="CUTICULAR PROTEIN 12A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00379">
        <property type="entry name" value="Chitin_bind_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00233">
        <property type="entry name" value="CHIT_BIND_RR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51155">
        <property type="entry name" value="CHIT_BIND_RR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0193">Cuticle</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026286290" description="Cuticle protein 6" evidence="4">
        <location>
            <begin position="32"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="160"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="169"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010845-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="349" mass="36176" checksum="A60F07316156DD98" modified="2020-08-12" version="1">MTVNMSPLGGGRWRCLASLLIFTILARIVRAEQEVIVAIDEPGKTQYHEANFNTSSYQYGYEVGPNGQFHHETRGPDGVTYGCYGYIDPNGQLRVTHYVADTHGYRVVEPNRPVEIFVDAPTQYSNSIAEEEPQERRRGELRPWTELYLPKGCGMFPGGMRPDGGSGGASPPTGGISLGAGVSTAGISGTASTSHTGPNHNVMGTIGGSIGSSTGVQTSAGVNASATGLGVTVDHTHTSNVTVTGESVQVTHGGQNTIDSQQVTVAGTVGVAVGVEAGLIDSSLNHTGHVSVGSIDVSSATAVGAGIVANHTTIGGVAQLTHNASISIPTVAPGSMPHRKLTQRIIRIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W2L9</accession>
    <name>A0A6E8W2L9_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="10">FIP-RBD domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cleavage furrow</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Midbody</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Recycling endosome membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8W2L9"/>
    <dbReference type="EnsemblMetazoa" id="ACON010445-RC">
        <property type="protein sequence ID" value="ACON010445-PC"/>
        <property type="gene ID" value="ACON010445"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002532"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON010445"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037687"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032154">
        <property type="term" value="C:cleavage furrow"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030139">
        <property type="term" value="C:endocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055038">
        <property type="term" value="C:recycling endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032465">
        <property type="term" value="P:regulation of cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.2440:FF:000003">
        <property type="entry name" value="Nuclear fallout, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.2440">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037245">
        <property type="entry name" value="FIP-RBD_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019018">
        <property type="entry name" value="Rab-bd_FIP-RBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057316">
        <property type="entry name" value="Rab11-FIP3/4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051977">
        <property type="entry name" value="Rab11-interacting_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15726:SF7">
        <property type="entry name" value="NUCLEAR FALLOUT, ISOFORM J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15726">
        <property type="entry name" value="RAB11-FAMILY INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25450">
        <property type="entry name" value="Rab11-FIP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09457">
        <property type="entry name" value="RBD-FIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144270">
        <property type="entry name" value="Eferin C-derminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51511">
        <property type="entry name" value="FIP_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0967">Endosome</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="FIP-RBD" evidence="9">
        <location>
            <begin position="237"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="10"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010445-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="301" mass="35123" checksum="9AF91FB7DE5D877B" modified="2020-08-12" version="1">MYSDVSLEDVQDINHKLEILQRQVTNLADTQSNVDDRTSRTKTEYAVLQARYHMLEEQLRETELRAEERLAEEQKRHRELLARVEREAKLQNENCQIRIRTMEMEVTSLREEIQRLRLQSDKQAADLHATEEKLEKARDSLMISQQDLAEARAEEKKHRAEKQAAEELMVELGKECERLRSERGPALPTTSPESLRLEELHQEMDELRQKNKSLEEANEELQAMMLTRSIEEGRNLLNGTSNSLAQELEAMSQNQDTVDSTTLASLTQEKEEENRRLKHYIDTMLLNVVENYPQLLEVKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W4B4</accession>
    <name>A0A6E8W4B4_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Myosin tail domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_049465530.1">
        <property type="nucleotide sequence ID" value="XM_049609573.1"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON011138-RB">
        <property type="protein sequence ID" value="ACON011138-PB"/>
        <property type="gene ID" value="ACON011138"/>
    </dbReference>
    <dbReference type="GeneID" id="120959816"/>
    <dbReference type="CTD" id="41955"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011696"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON011138"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041720"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032982">
        <property type="term" value="C:myosin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031032">
        <property type="term" value="P:actomyosin structure organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002928">
        <property type="entry name" value="Myosin_tail"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615">
        <property type="entry name" value="MYOSIN HEAVY CHAIN, NON-MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615:SF36">
        <property type="entry name" value="MYOSIN HEAVY CHAIN-LIKE, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01576">
        <property type="entry name" value="Myosin_tail_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myosin tail" evidence="4">
        <location>
            <begin position="948"/>
            <end position="1246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="191"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="327"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="366"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1509"/>
            <end position="1628"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1663"/>
            <end position="1691"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="860"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="937"/>
            <end position="1494"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="1703"/>
            <end position="1753"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="27"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="96"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="198"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="266"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="417"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="447"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1515"/>
            <end position="1530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1559"/>
            <end position="1569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1614"/>
            <end position="1628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1663"/>
            <end position="1679"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON011138-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1788" mass="196003" checksum="0C265309BEAACFB0" modified="2020-08-12" version="1">MRPIISAPSAVVQSPSSSRIFPPATVTAAGSGQNGTNGQQQQQTQADPGNGVDQPPLLQNGSNNNNNPSNGFGIVKKVKKEPSPSPLSTAGEAPLVKPPPVANKPPPVGGKLSSWRQYLPSGMGGGGGGTTVASASKNATGAVKNDKPTHAQKATVVDENNNEMTAVLMSEGMTTNNGNGCHSAVIKEEGAAAAATNGCVQNGENQPAESKENGTTTEKLSNGGAVKSEEKLPPPPPPSASQRKSNGERVNGTPPLPPATVNGTGARKESVKSEIKVKIAPKGEEKKEPSLVSTAIGKVSTKTLELKRSPSGHRLTTASSVVTTTVVEENGTEKPTNGVGGGVEDGKEKKRSAYLLTDEAGKSLAKGKAVPKTASTEKSGPIGVRLSLKPSRSVTPDRTEKSSTPIRAARASPVRGTIEDGMPKEKSPSERRILGEAVRREKTPLRSASSKSLSAKGDSTSSKSTTTIEVKEVGDGDVTTSVLTSTATVPSSTSSASSSRPVVKVRKVIKKIIKKKPKAAAEGTDAAVAAKEEQAANAEVAKKEGDSHSPPALVGAVETTITHEMADSAVVKGMVEITKMEIAKEKEKEKKKSAVKSVPVKEEKGLKDLKDAPKSTEKFEEVAVVSGKEQQHIERKHWAIKKRSMTDRALSCGPLEDDRSGAMLEQFLLSLATIPQEIAVDLPAGATLPRFGSNRLLARSASQYLSSKIHDFLRRTDHVMQDWRNLGRTLGRRDDYGVRGTIGDVSGLGGTVRSRSVSNIIARGLQLLREQSSSPARRSSVVSRSSSRSSFYCELPAGDGKGGPAGLALRRRRIRNIGERVVEDDDDDCSTVVDMSEELSDITSELTEEHSSSNLITERLAAETSERLRLEKEVKEYESKYRHLQESSEKMEMELLCAKSELNCDFDDCSTLGEYDSTGDGADLPLGGGPADGAELAKSYRLRYERVARELEFTKKRLQTQHEHDLEQLVGLKKQLEKKLADAYEEVEEQRQVVAQWKRKAQKMTNEMNDLRMLYEEQNSRNNLLEKRQRKFDAECQTLQDSARQERQAKERMAREKDVLMAEKCKLEQTVSDIRLELELKEEKVTALQQELDEMAFGGGTEEEIAQLKRQKNELDRRCKEQDEELDEMAGQIQLLEQAKLRLEMSLETSRKEARKEAQQRDDEMEEIRGASYKKIKSLECQLEQEHEERTQLLRDKHELERKLASLEDQDRAERAAEEAMVQRLKRDARKYRALLRDAQSQLDRAKGDSASKALVRQLRNQLEDAESARVAALKARQVLEGELQDAQLLFEETQRARNEAEDRATVAQRDRAELQAQIDENEEEMAELMKKYSATVKQLSSEQSLIAEYELRVSELEGEKKSLKEQVVELATRLESVETIGEPSNSMAFKRLELRTKELESRLEFEQATRARIEIQLARHKDSLEKLQGELSQARNREAQAQDALKKGQKTVRELRDELSTLANRDQEGLLKRKELEKRIETAESETASARADLRLALQRIADLQQAMEEEGDSYQSDSDTSDSSSSMDSFHESTVTRKSPSVGSGDHFGTTNGGSSRGSVGSLASSTRDGRKESNNPRIRPSLFGSVRRKKRLHPTIKEEDESYLTDDQAGTEPSTSSLPQVSQTPDLLTSLETSQPMREDSKAAADTEHVETVAEACRRMSESNMQREEAAAEKEPQSALSLPPVPSGEQCEFPFDIDTLKSIKEKIHSLNQIIRDESHESSTEIDLVDLKNLKNQIHEFKKAIECSRSRSMDKLVNGGGSALLAPGIITTSASTGSASTGRPLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W4N3</accession>
    <name>A0A6E8W4N3_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Putative inorganic phosphate cotransporter</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">May be an inorganic phosphate cotransporter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Sodium/anion cotransporter family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040240003.1">
        <property type="nucleotide sequence ID" value="XM_040384069.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8W4N3"/>
    <dbReference type="EnsemblMetazoa" id="ACON012251-RB">
        <property type="protein sequence ID" value="ACON012251-PB"/>
        <property type="gene ID" value="ACON012251"/>
    </dbReference>
    <dbReference type="GeneID" id="120960111"/>
    <dbReference type="CTD" id="36865"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006358"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON012251"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034775"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006820">
        <property type="term" value="P:monoatomic anion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17318">
        <property type="entry name" value="MFS_SLC17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000144">
        <property type="entry name" value="Picot, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000003">
        <property type="entry name" value="Solute carrier family 17 member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050382">
        <property type="entry name" value="MFS_Na/Anion_cotransporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11662:SF247">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11662">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="9" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5" id="KW-0769">Symport</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="95"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="123"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="218"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="322"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="380"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="412"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="445"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="14">
        <location>
            <begin position="22"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="478"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="495"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00054632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00068450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON012251-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="515" mass="56659" checksum="EFE2A82A1A0DB399" modified="2020-08-12" version="1">MTVSKEYLANAEPEKPNQRFGTRHYIVFMLFLGMANAYVMRTNMSVAIVAMVNQTAIEKDAEVFDDECPDTDYGEDTHDSGQDGEFIWSTSMQGYILSSFFYGYVITQIPFGLLAKRYGAMRFLGWGMLINSVFAFVVPVAARQGGAGWLIAVRFIQGLGEGPIVPCTHAMLAKWIPPNERSRVGSIVYSGAQFGTVISMPLSGLLADHGFDGGWPSIFYVFGIIGTVWSIAFLLTCHEDPVTHPRIREDEKKYIQQSLWGNASVHIPPIPWTSIAKSLPFYAILLAHLGQNYGYETLMTELPTYMKQVLRFSIKTNGVLSALPYLAMWLFSIVVGWVADWMLTSGRVNHTWTRKISNSIGQYGPAIALIIASYTGCSRALTVAILTVGVGLNGGIYAGFKINHLDITPRYAGVLMAFTNCSANLAGLLAPIAAGNIIEGKPTIAQWRIVFVIAACVYLFTATFYNVFASGARQFWDDPENDEPQKPSIEAPSHSEANGNNGTTLTHRTVNEQTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W529</accession>
    <name>A0A6E8W529_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Outer dynein arm-docking complex subunit 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Tetratricopeptide repeat protein 25</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8W529"/>
    <dbReference type="EnsemblMetazoa" id="ACON011462-RA">
        <property type="protein sequence ID" value="ACON011462-PA"/>
        <property type="gene ID" value="ACON011462"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_003156"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON011462"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037565"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040111">
        <property type="entry name" value="ODAD4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23040:SF1">
        <property type="entry name" value="OUTER DYNEIN ARM-DOCKING COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12895">
        <property type="entry name" value="ANAPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0966">Cell projection</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="24"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="92"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="140"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="140"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON011462-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="255" mass="29478" checksum="A2503296E5A3137B" modified="2020-08-12" version="1">MNKPMNKKRQKGSKPVIDEIEVAGKNLRDEALKYMKVRNYTKALGLYDQALEMNNNDQNALVARSRCFLQLGEPGKALQDAETALVLDKNNIRAIYQKAEALYYLGQFEHSLMFFHRGLRLRPELASFRLGVQKTQEAIENTIGNNTKNQPQKKPTKVEKPKTAKRNQLSREELEKRASRRLLGDLYIDKEYLENLIKHPDLRKADTNTEGVSEYAKDAINFLNNRQEFWRQQKPCTSLNAKKINGANGMLPRWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W6F3</accession>
    <name>A0A6E8W6F3_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">G protein-coupled receptor kinase</fullName>
            <ecNumber evidence="10">2.7.11.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[G-protein-coupled receptor] + ATP = [G-protein-coupled receptor]-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12008"/>
            <dbReference type="Rhea" id="RHEA-COMP:11260"/>
            <dbReference type="Rhea" id="RHEA-COMP:11261"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43176"/>
            <dbReference type="ChEBI" id="CHEBI:68546"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.16"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.-" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8W6F3"/>
    <dbReference type="EnsemblMetazoa" id="ACON012026-RA">
        <property type="protein sequence ID" value="ACON012026-PA"/>
        <property type="gene ID" value="ACON012026"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010190"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON012026"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031315"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001664">
        <property type="term" value="F:G protein-coupled receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004703">
        <property type="term" value="F:G protein-coupled receptor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01240">
        <property type="entry name" value="PH_GRK2_subgroup"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08747">
        <property type="entry name" value="RGS_GRK2_GRK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000118">
        <property type="entry name" value="G protein-coupled receptor kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000068">
        <property type="entry name" value="G protein-coupled receptor kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000239">
        <property type="entry name" value="GPCR_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016137">
        <property type="entry name" value="RGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036305">
        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24355:SF18">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24355">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR KINASE/RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00615">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00717">
        <property type="entry name" value="GPCRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00315">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50132">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RGS" evidence="14">
        <location>
            <begin position="16"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="153"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="15">
        <location>
            <begin position="417"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="12">
        <location>
            <begin position="519"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="489"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="280"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009793"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR600239-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON012026-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="684" mass="78219" checksum="6840E6BAE0CD24D3" modified="2020-08-12" version="1">VRSVMHKYLEKENEVNFDKIFNQVLGYLLFRDFCDNVSEEPVPHLKFYEEIKAYEKTECHDERRKQARDIYDNYIMKEMLSHTHEYSKEAVAHVQKYLMKNEVPVNLFEPYIEEIFHHLRGEPFRKFLESDKYTRFCQWKNLELNIQLTMNDFSVHRIIGRGGFGEVYGCRKADTGKMYAMKCLDKKRIKMKQGETLALNERTMLSLVSTGVDCPFIVCMTYAFHTPDKLCFILDLMNGGDLHYHLSQHGVFNESDMKFYAAEVILGLEHMHKRFIVYRDLKPANILLDENGHVRISDLGLACDFSKKKPHASVGTHGYMAPEVLSKGTPYDSSADWFSFGCMLYKLLKGHSPFRQHKTKDKHEIDRMTLTMNVELPESFSKELRDLLEGLLQRDIDKRLGCKGGGAEEVKCHPFFAGIDWNQVYYQKYTPPLIPPRGEVNAADAFDIGSFDEEDTKGIKLTEQDQELYRYFPLTISERWQQEVAETVFETVNTEADKLEQKRKAKQKQRFDADEKESDCILHGYIKKYGGSFASVWQTRYAKLYPNRLELHTESGSTKPELVFMDQIEEVSPDFVQFKNEQCVQVRFRDGIRDGKLILTNSDEIGLKEWALSLRAAHKESQELLGSMAKKAGKIYGTEGQRGAPDARQLAGSGMVLPGGGGGTATGGGVIAAGGPKNANGGSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W6T2</accession>
    <name>A0A6E8W6T2_ANOCL</name>
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        <submittedName>
            <fullName evidence="3">2-arachidonoylglycerol hydrolase ABHD12</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
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            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8W6T2"/>
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        <property type="protein sequence ID" value="ACON012189-PA"/>
        <property type="gene ID" value="ACON012189"/>
    </dbReference>
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    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037399"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004622">
        <property type="term" value="F:phosphatidylcholine lysophospholipase activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:monoacylglycerol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006660">
        <property type="term" value="P:phosphatidylserine catabolic process"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277">
        <property type="entry name" value="ALPHA/BETA HYDROLASE DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277:SF194">
        <property type="entry name" value="FI04476P"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="alpha/beta-Hydrolases"/>
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    </dbReference>
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    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="Serine aminopeptidase S33" evidence="2">
        <location>
            <begin position="163"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblMetazoa" id="ACON012189-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="392" mass="45397" checksum="47061E31231F0559" modified="2020-08-12" version="1">MMPLCRKIGRCTCAVAVLLFLVGFVILPIIFKYSYALQKSILFLTFITYPPNLDLKRPEKSGLYATRNFYVNHHDHEEDLEVNVAVWHVLPLDLVRRYAKELHVDEVSGKRGSIRSIVADEKHHTGDRYKGIEKVLRSDGFQLTDEKHQRDLFEETLRATTNDVVLYLHGNTASRGAPHRVELYQMLRALNYHVIAIDYRGYGDSANLSPSELGVVYDALAVYQYITSITNNPVYLWGHSLGTGVSTHLLSLLTEMSLPGPKAVVLESPFNNIKEEICAHPFSKLYRHLPWFDYLISRPMYKNKLRFESDQHIAEFRQPVLILHAEDDLVVPFELGYKLYRKALDTRGKSWGPIEFHRFEKSSHYGHKYICRAPNLPEIIVRFFHAYRDAQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W751</accession>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
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    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
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        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
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                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8W751"/>
    <dbReference type="EnsemblMetazoa" id="ACON013481-RA">
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        <property type="gene ID" value="ACON013481"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009241"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON013481"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030170"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="302"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON013481-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="335" mass="37914" checksum="F482FB0308FB7C19" modified="2020-08-12" version="1">MVYESDFYSTRRVGSSYTRPTISSYTVTTPLRYSGVPRFNTITTTTTTYRTPMPYVAHKRLVPVTRVVTSPPRIVVSPMRVLGSPVRVISSPVRVLGSPMRTVRAIRSPARVIVSPARVVTIRSPYLRPSIINKEFDRIERKYRASPVSSALEQYYNSPSYLEFEDAKSDIRNSTALLLRQLNDPVPRLMAPIAQAAPEPNPKKWVYDPFSTHNRLNSDTYVKSHITDPIRSVRNDIEAMARYHSPASRYVGKNHLASTRIIGSQAYPKTKPRIYNYDTAKVGKDVNVMSYYKSNRTQAKADVEEVKETETKTEVRKSKLGDKKGIYSSDVNLAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W756</accession>
    <name>A0A6E8W756_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein anoxia up-regulated</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
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            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8W756"/>
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        <property type="gene ID" value="ACON013481"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001105220">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="21"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="24"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="61"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="90"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON013481-PF"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="130" mass="14399" checksum="0D97B33C8243E576" modified="2020-08-12" version="1">MVYESDFYSTRRVGSSYTRPTISSYTVTTPRYTTVETSTPRRDESYTYSKTYKTVTESANSSNPYSRPSTSTSSYSSTTERNSRGGPGGYSYSTERTSTTGGGPGGYSYSSTTSGRLPHGTTYRHYSYRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W768</accession>
    <name>A0A6E8W768_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Calpain catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
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            <taxon>Metazoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
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            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8W768"/>
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        <property type="protein sequence ID" value="ACON012068-PB"/>
        <property type="gene ID" value="ACON012068"/>
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    <sequence length="832" mass="94033" checksum="34AC556811CEF638" modified="2020-08-12" version="1">MDELKGLLSGAGKQLFDAAGQAITSAATEYIGSVINEMFVKKETDTKRFLPSIKNMKVLGDRNAGQRSSFDVQDFYQIRQQCLDNGTLFEDPEFPASASSLMYSKRPDRHYEWLRPHEICDGPEFFVEGFSRFDVQQGELGDCWLLAACANLTQDHKMFLRVVPEDNSFEDGYAGVFHFRFWQYGKWVDVVIDDRLPTYRGQLIYMRSSEQNEFWSALLEKAYAKLHGSYESLRGGTTCEAMEDFTGGVAEMYDLKDQTPPNLFEIIEKGFKRNSMFGCSIEADPHVLEAETPEGLIRGHAYSITKIQLVDIETPGRSGKIPLIRLRNPWGNEAEWNGPWSDKSPEWRYIPDEQKQELGLNFDHDGEFWMSYRDFTRYFDRMEICNLSPDSLSDDEMTRGKISWEMSMFEGEWAVGTTAGGCRNYLDTFWHNPQYVIRLDDPDEDDEEGNCTVIIALLQKNRRSRRNMGVECLTIGFAVYRVTERDLAQKPLKMNFFKYNASAARSPAFINLREVSCRFKLPPGTYVIVPSTFEPNEEGEFIIRVFSETANSMMENDDNVGVGDLDDRIAPDVPGYVQPSPQRQAMERLFLDVAGADGEVDWVELKRILDHSFRDDLPKTSSLNGLNRQTYASQQNAEAGPAGPGGGIEDLLSMLLNMCCKGVLGGDGDGGGGGGTHPSGPANLESQIVPNQPLHDNISSEGFSKDVCRAMVAMLDVDHTGKLGFEEFQQLLTDIAKWKAVFKLYDTEGSGRLSPFQLREALNSAGYHLNNRILNALVHRYGSRSGTIPFDDFIMCAVKIKTMIEIFRERDTDGTNQATFSMDEWVEKTLYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W8X7</accession>
    <name>A0A6E8W8X7_ANOCL</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
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            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8W8X7"/>
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        <property type="protein sequence ID" value="ACON028482-PB"/>
        <property type="gene ID" value="ACON028482"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026237392" evidence="2">
        <location>
            <begin position="22"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="48"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="118"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON028482-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="138" mass="14565" checksum="1737A46266743440" modified="2020-08-12" version="1">MEPKWLLLAVFCLFCQNLSLGSLTTDDDVASNANDTKQWRILQAPRVTTPSSGRMTTTTRRTPTTTVAKGKTTARSAQPSNAAGRTSTTTKRSGQLLAGPKAPTTTSRTTKTALRVSSGPNGKVSTTTAKSIQVHVAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W8Y9</accession>
    <name>A0A6E8W8Y9_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Microtubule-actin cross-linking factor 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON029051-RE">
        <property type="protein sequence ID" value="ACON029051-PE"/>
        <property type="gene ID" value="ACON029051"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007811"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON029051"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040800"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030056">
        <property type="term" value="C:hemidesmosome"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:intermediate filament"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045104">
        <property type="term" value="P:intermediate filament cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042060">
        <property type="term" value="P:wound healing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21189">
        <property type="entry name" value="CH_PLEC-like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00176">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000260">
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<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
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            <taxon>Arthropoda</taxon>
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            <taxon>Anophelinae</taxon>
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    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain>Mali-NIH</strain>
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    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
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                <consortium name="EnsemblMetazoa"/>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="3">Ngousso</strain>
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</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WCN0</accession>
    <name>A0A6E8WCN0_ANOCL</name>
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        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
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        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
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    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
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    <reference evidence="13" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
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            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">Ngousso</strain>
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    </reference>
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        <subcellularLocation>
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        <text evidence="2 12">Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family.</text>
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</entry>
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    <accession>A0A9I3AN35</accession>
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        <name type="scientific">Anopheles coluzzii</name>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="3">Ngousso</strain>
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        <property type="nucleotide sequence ID" value="XM_040365413.2"/>
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        <property type="entry name" value="ITPR-bd"/>
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        <property type="entry name" value="TESPA1-like"/>
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        <property type="entry name" value="ITPR-INTERACTING DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
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        <property type="entry name" value="SPERM SPECIFIC ANTIGEN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="KRAP_IP3R_bind"/>
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            <end position="725"/>
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        </source>
    </evidence>
    <sequence length="726" mass="79890" checksum="15FAD8F368ED7212" modified="2023-06-28" version="1">MFEVIFLERMEIEHLLRYLDTDREGKNGEERTSQLHRIRSRHIIRSRVRYWLNDIVDEMRKKSPASVQKWVDSIQIPSGAGAGEQSTGSDAQIATGQDNDPSAYVSNYTRSDQLAAGTVPQTEGHDRPDRGWTGATETGEPLETDSEEERQAFAYEHQQQQSDQPDRVLIDSDQNKHSSTSSTTNHGLPSEPPISSLATSTPKAIKPDEGAKQMPVSEGAAPSGLSIRNFLSKKSLLGKFNRSGSNLEQSPPTTTTTTAGEGVAEMGNPEHVEHGRHSTEKGEVTAAPSPFVVGKSKINEFYDKLNMNKAKYGLMKAKNLDIRNMLSGGSRESADARKAHEAQPVQSGADEPNPVESGFEGELEPPASESEHERSVSPQHHEPLEVSVVDVDPTDPYEFSFDESFPKSDELIPGMISEEDEEEKENILDGASVLPGYGYDAYLNPPKPKTHRMGIGRIGRSSSENPRATARRYNLMEIGRSFSEMNDDDDSFAISECNNSCPNPSSLNTSSSINEHGSVSNLTRSVPVSPIPRTSSKLSIQQDHPSQHQLHQQQQPLHQQQSSPRRRSTMLVKDSSLQSDSSRCSSVESLLNARKPDPEKILLNLGFGPAPHSTDVLAKIPKRFLKPSQVRGVDTEAFLRQQQLSMHIHENSVLGYRGLVGNPHIPPSMIVAKIMERFQENDSKTRLTAAGVPLLQPDSRNSSGPPSPVPMSRHASLATADGRLKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9I3AN66</accession>
    <name>A0A9I3AN66_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2023-03" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A9I3AN66"/>
    <dbReference type="EnsemblMetazoa" id="ACON005614-RB">
        <property type="protein sequence ID" value="ACON005614-PB"/>
        <property type="gene ID" value="ACON005614"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039893654" evidence="1">
        <location>
            <begin position="17"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005614-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="148" mass="14038" checksum="AD7010636A5E4EC6" modified="2023-06-28" version="1">MKLFVVLMALVAAAVAAPAPQFGFGYPGFGGGYRHHPRYYRPYPGPHYGGHYGGPVGGLLGGGGFSGSQANAQSASFNIGLGGISGSFSNSNANSFGGGPGGGFGGSGAQASAQSASFGGGLGGFGGSASNAQASANSFGGGGGGLFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9I3BDD1</accession>
    <name>A0A9I3BDD1_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Proline dehydrogenase</fullName>
            <ecNumber evidence="5">1.5.5.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2023-03" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Converts proline to delta-1-pyrroline-5-carboxylate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-proline + a quinone = (S)-1-pyrroline-5-carboxylate + a quinol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23784"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17388"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.5.5.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the proline oxidase family.</text>
    </comment>
    <dbReference type="EC" id="1.5.5.2" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A9I3BDD1"/>
    <dbReference type="EnsemblMetazoa" id="ACON030686-RA">
        <property type="protein sequence ID" value="ACON030686-PA"/>
        <property type="gene ID" value="ACON030686"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032403"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004657">
        <property type="term" value="F:proline dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010133">
        <property type="term" value="P:L-proline catabolic process to L-glutamate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.220:FF:000029">
        <property type="entry name" value="Proline dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.220">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029041">
        <property type="entry name" value="FAD-linked_oxidoreductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002872">
        <property type="entry name" value="Proline_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015659">
        <property type="entry name" value="Proline_oxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13914:SF0">
        <property type="entry name" value="PROLINE DEHYDROGENASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13914">
        <property type="entry name" value="PROLINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01619">
        <property type="entry name" value="Pro_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51730">
        <property type="entry name" value="FAD-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4 5" id="KW-0642">Proline metabolism</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proline dehydrogenase" evidence="6">
        <location>
            <begin position="42"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON030686-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="522" mass="59981" checksum="0FD8EFCB08F5ED3B" modified="2023-06-28" version="1">MPRRLMKVAQTILGEKLFTLLMKYTFYGHFVAGEDQQKIVPTLERLRSFGVKPILDYSVEEDLSQEEAEKREVEASVSSAGTNLSEDSADSLPQYSVDKTFADRRYKVQSARTYFYLNEATCERNMETFLECLDAVKGATFGTGITAIKLTALGRPQLLLQVSEVIMQARRYMMDLAGSTGNILTHHKTIQDLERYLGNVSDKAEVKQFLEKVTSDKDGILHLFPWTGIINEDCQLSETFRVPDPVTGQMRRLISKIPPKEEEMFRNMIRRLNTIVKTAQDLDVRIMIDAEQTYFQPAISRITLEMMRKYNTEKAIVFNTYQCYLKDTYKEVCTDLEQAKRQNFYFGAKLVRGAYIEQERARAAALGYEDPTNPSFEATTEMYHKTLTECLRRIRILKDANIDPKKIAIMVASHNEDTVRFAIEKMQEIGIHPEDKVICFGQLLGMCDYITFPLGQAGYSAYKYIPYGPVNEVLPYLSRRAQENKGVLQKIKKEKRLLLSEITKRLAKGQLFYKPKGKYVPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9I3BDD6</accession>
    <name>A0A9I3BDD6_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">arginine kinase</fullName>
            <ecNumber evidence="2">2.7.3.3</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2023-03" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7 9">Belongs to the ATP:guanido phosphotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.3.3" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A9I3BDD6"/>
    <dbReference type="EnsemblMetazoa" id="ACON030689-RB">
        <property type="protein sequence ID" value="ACON030689-PB"/>
        <property type="gene ID" value="ACON030689"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037636"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004054">
        <property type="term" value="F:arginine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004111">
        <property type="term" value="F:creatine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046314">
        <property type="term" value="P:phosphocreatine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07932">
        <property type="entry name" value="arginine_kinase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.590.10:FF:000006">
        <property type="entry name" value="Arginine kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.135.10:FF:000003">
        <property type="entry name" value="Three-domain arginine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.135.10">
        <property type="entry name" value="ATP:guanido phosphotransferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.590.10">
        <property type="entry name" value="Glutamine synthetase/guanido kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000749">
        <property type="entry name" value="ATP-guanido_PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022415">
        <property type="entry name" value="ATP-guanido_PTrfase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022414">
        <property type="entry name" value="ATP-guanido_PTrfase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022413">
        <property type="entry name" value="ATP-guanido_PTrfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036802">
        <property type="entry name" value="ATP-guanido_PTrfase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11547:SF38">
        <property type="entry name" value="ARGININE KINASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11547">
        <property type="entry name" value="ARGININE OR CREATINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00217">
        <property type="entry name" value="ATP-gua_Ptrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02807">
        <property type="entry name" value="ATP-gua_PtransN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48034">
        <property type="entry name" value="Guanido kinase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00112">
        <property type="entry name" value="PHOSPHAGEN_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51510">
        <property type="entry name" value="PHOSPHAGEN_KINASE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51509">
        <property type="entry name" value="PHOSPHAGEN_KINASE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 8 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphagen kinase N-terminal" evidence="10">
        <location>
            <begin position="6"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphagen kinase C-terminal" evidence="11">
        <location>
            <begin position="118"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="121"/>
            <end position="125"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="279"/>
            <end position="283"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="308"/>
            <end position="313"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON030689-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="355" mass="39775" checksum="9B94EDE62C7DD2EF" modified="2023-06-28" version="1">MVDAAVMEKMEAGFAKLAASDSKSLLKKYLTKEVFDALKNKKTSFGSTLLDCVQSGFENPDSGVGIYAPDAEAYSLFADLFDPIIEDYHKGFKKTDKHPASDFGDVNAFGNVDPTGEFVVSTRVRCGRSMEGYPFNPCLTEAQYKEMEGKVSTTLSGLEGELKGKFYPLTGMDKAVQQQLIDDHFLFKEGDRFLQAANACRYWPSGRGIYHNDNKTFLVWCNEEDHLRIISMQMGGDLGQVYRRLVTAVNDIEKRIPFSHHDRLGFLTFCPTNLGTTIRASVHIKVPKLAKDYAKLEATADKYNLQVRGTRGEHSEAEGGIYDISNKRRMGLTEFQAVKEMYDGISEIIKIEKSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABM2AD95</accession>
    <name>A0ABM2AD95_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ARID domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON001786.R247">
        <property type="protein sequence ID" value="ACON001786.P247"/>
        <property type="gene ID" value="ACON001786"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd16865">
        <property type="entry name" value="ARID_ARID1A-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.60">
        <property type="entry name" value="ARID DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001606">
        <property type="entry name" value="ARID_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036431">
        <property type="entry name" value="ARID_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021906">
        <property type="entry name" value="BAF250/Osa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033388">
        <property type="entry name" value="BAF250_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12656">
        <property type="entry name" value="BRG-1 ASSOCIATED FACTOR 250 BAF250"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12656:SF5">
        <property type="entry name" value="TRITHORAX GROUP PROTEIN OSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01388">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12031">
        <property type="entry name" value="BAF250_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01014">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00501">
        <property type="entry name" value="BRIGHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46774">
        <property type="entry name" value="ARID-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51011">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ARID" evidence="6">
        <location>
            <begin position="717"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="826"/>
            <end position="1356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1405"/>
            <end position="1430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1556"/>
            <end position="1585"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1615"/>
            <end position="1646"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
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            <begin position="1794"/>
            <end position="2055"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
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            <begin position="2480"/>
            <end position="2664"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="7"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
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            <begin position="33"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
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            <begin position="82"/>
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    </feature>
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            <begin position="173"/>
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    </feature>
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            <begin position="186"/>
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            <begin position="259"/>
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            <begin position="1943"/>
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        <location>
            <begin position="1985"/>
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        <location>
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            <begin position="2594"/>
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    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
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            <dbReference type="PROSITE" id="PS51011"/>
        </source>
    </evidence>
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            <dbReference type="EnsemblMetazoa" id="ACON001786.P247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
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    </evidence>
    <sequence length="2664" mass="277247" checksum="21A6A792BB066401" modified="2025-10-08" version="1">MRPTPSPTGSGSGSRSMSPAVGQQSISMPPRPSSSHSQAPNQQQQGPNHQHGGSNSNLVHGQQQQQQTQGPPPGTPEHHARVASPNRPPSASAQQQQQQLPPVSSAPAGGPALPPSSQAGAAGGSAAAASAMAAQGYPAQPPHPPHMHGGPYKMGPGGPGGMPPGGPGQQMSPYPPPPHQQPPPQSQQYSPHSPASYSPRPQYPGAYGPSPPVPGPPPPPGTQSAQGPPGSGGPQPPHPPPPGGYPGQHRMPNHVGAGGPPPPPHSQYPPHQYPHQPPSPWGPPHTQQAGGPGGAGAGHVNNHVQQSQQGQGGPPTPGGAGGPRQVKGMPPGPPGAGGVHPQSPQQQQQQHSQHHQPPPPHHQPHHQAHHQSPHHPGGQSQQPPPQSGSPQPLNYLKQHLQHKGAYSGAPSPTPPPGQGYGNGPGMHPPMGPPHHMGPPMGLPPPPNASSMGPPPPAGTPPNQSPSGAGGSGALGHPQHPSQQDGGHPPSPHGQQQQQQPPSQPQHPGQQQQSQQSQQQPPHSPHHHPHGPPPPPHQHPGHHPGADVPPQPPHHGLLAGAGQDNGIGNAAGGPGAGGGAGGGPHHHHHPHHHHPVTSLVTTGPDGAPLDEASQQSTLSNTSVASGEDPTCSTPKSRKNDHYNQSHLTNTNTPSSSNASPGPHNAHEDFELNSSPTSWPRTPASPVFNSHVPPTPETPFRSTKQKSDSLNKLYEMDDKPDRRVFLDKLLAYMDERRTPITACPTISKTPLDLYKLYVYVQERGGFVEVCKVTKSKTWKDIAGMLGIGASSSAAYTLRKHYTKNLLPFECKFDRGGIDPGPIIAQVEAGSKKKSAKTTSVPSPGSSNSQDSFPAPGSSSASMDGYGPYPGSGYPPGSTPDYVQQQQQQGAAGGPPGGPPTPGAPAGPGQQQPPQPQQRPPSQQSGTQSPHLGSAPPASGDNISVSNPFDDPVGPQRPPYQQGAPYPPVSRPQGAPYQGQPGGYGQYGAPDQYGPGAGTPPGQYPPPPGQQGQYPPTNRPLYPPYAPPEAGEGGTPPSSAAPGPPSAADPYRGYGSSPYPPPPQQRAYPPQQQPVVSAAPGVPPAGTPPIPGQTGGAAQGQVPPGGGPPSQQQQQQQQQQQPQQAPPPQQQPGAQGQPGAPGGGYPAPVGQPGVPQSAADYYRQQQEQPNQPRRHPDFEKSQQPYPPYQQRPQMYPGGWQAGPGGQYRGQYPPQSPQQQWAHNGPRPSGPPGPPGAGVPPGGPPGAPGQWGATADVNRYPPSQQQPPYPTHQQGQQQPWNQMPPTSQNSPIRPPMRGGKPYPPMAGAGGPGVPAPPGTMPGMPPASINTPPQPPGVVGGPPGAAPGAAGGPVVPVSGGPPVVPSPGGGVVVPGSGVPSVAGSVGSAAGTPGATPVPNASPAVAVKTQPTVQGGGGQVQQAGPNFPPAGSGGVQQKRDIVFPSDSVEATTPVLYRRKRITKLDIGQTDAWRIFMALRSGLLVESTWALDVLNILLFDDSSVAYFGLAHLPGLLNLLLDHFQKSLCDMFESGKREGTTNRFSVEGSDRAALLDQMRDIDEEGEEAQREEEATGRNRSSSIAVTTTTTETTIKLEPASPMMTTTMTTTTTTTMIKKEKLENGLPEGGDASLHHNGTRDGGDSKAASNGTGATIDRRRSAQCYARRNIKRCDSSVELDLGAVKNPPNPEERTLILTSTTNYTMTSRKGRPVKIHPAEDDVFVLDHRRDWDRASDYQYQRDTEVGGDPWTAGHTDPDPHDYIMDTFRAEFVNIPFAKYIKTSAKYAEAAKANGGRNGKRIAAANNNNNCSSNNNNNNNSVKCTSKNEREEPQQENAAVENSAAAAATTATVKNTSDEAPPTSMEVEEEEELLSPPKRRKEDPHLSPAATPTLRTRKDHHIARLMNGESGEEEEGQAKEKSDSGLVDKVVKNCIVKKEKAAEEKEASETGDKTTAAGTKGGTAPAPSPMDADCREIDMELEKSSTLSNGPQEAGAKDHRDGSESRTSTTPDPEQKKLPKVEANQPPPSPSAALKSSEEKMDVDEEAAAAGEQQKEEDSATTTSAEVATKAFDIAETVRDPARVLKRRRMSDYEDECYTRDEASLYLVTEGQDALARRCVAISNILRNLTFLPNNETEFSKSSRFLAILGKLLLLNHEHPLRTKKTRNYDREEDADFSDSCSSLQGEHEWWWDFLVQIRENMLVATANISGQLDLSRFDEPISRPILDGLLHWAVCPSAHGQDPFPTLGPNAALSPQRLALEALCKLCVTDANVDLVIATPPFSRLEKLCSVLTRHLCKNEDQVLREFSVNLLHYLAAADSSMARTVALQSPCVSYLVAFIEQAEQSALGVANQHGINFLRDNPDSMGTSLDMLRRAAGTLLHLAKHPDNRPLFMQQEQRLLGLVMSHILDQQVALIISRVLYQCSRGTGPLTTMEIESGGKAFTASNSSSTGAAAAASAANSTASSVMEAKGGATDAKALLSASSMSTNGGSIAGGPSSPSATHASNSSASTPNGAASNAVTAATNGQAASDEFATPSTPSATIAANESADSKAASANASSPAGPSYEESGASSPTTPAEHGASSMANMGTSSPAGIGTEVVNPSTGSAATNNTSNSNCSNSSSSNSSSSSSSSSSSGNTVPTSPSAVTAAAAATSPAASVAPSSQHPITASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V1A0</accession>
    <name>A0A6E8V1A0_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="13">C2H2-type domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8V1A0"/>
    <dbReference type="EnsemblMetazoa" id="ACON002082-RB">
        <property type="protein sequence ID" value="ACON002082-PB"/>
        <property type="gene ID" value="ACON002082"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_003239"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002082"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039439"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="Zinc finger protein 362"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000648">
        <property type="entry name" value="Zinc finger protein rotund"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001172">
        <property type="entry name" value="Zinc finger protein rotund"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001678">
        <property type="entry name" value="Zinc finger protein rotund"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24379:SF121">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24379">
        <property type="entry name" value="KRAB AND ZINC FINGER DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="62"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="90"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="118"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="148"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="179"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="196"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="346"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="423"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="213"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="233"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="353"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="400"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="423"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002082-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="563" mass="58223" checksum="B1FB1737A5184CBF" modified="2020-08-12" version="1">MMAPVFGQLLLASEEGKINPASIPVKREKSEESDDLDVKIFSAKANLELHGGTLQMRETKPYKCTQCTKSFANSSYLSQHTRIHLGIKPYRCEICQRKFTQLSHLQQHIRTHTGDKPYKCRHPGCPKAFSQLSNLQSHSRCHQTDKPFKCNSCYKCFTDEASLLEHIPKHKDSKHLKTHICQLCGKSYTQETYLAKHMQKHAEKEEKRRNRAAGGGGGGGGGGSGGSGGGAGAAAASGANASAGSGAANGGSNNASSNANASNANNNSGAGSPNGGGAGGGAATAANAAAVAAAAVADAAAVGLAGLAGLGLNRMGGMVVGNAAAAAAAVGLEHPYWPKVSPDSAASLAEAMQQQQQQQQQQQQQQQQQQQQQQYDFSSNGGSGAGGNAANDPQHHRNLNGSSSSANSADEVGEDLVVIRHNNSQQQQQQQQHVNNQSNSSSAASNTSAFTPINVMPPHLNSLAAAHQHHQQQLVAAAQQRAAAAPPSYLYDAISFQNQKAINQNAAAVAAAAANGFPNQLISLHQIRNYAHQPGGGGGGGGGGGLLSGEHLLMGGGVGKVEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V1B0</accession>
    <name>A0A6E8V1B0_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Nuclear receptor subfamily 2 group B member 4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nuclear hormone receptor family. NR2 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8V1B0"/>
    <dbReference type="EnsemblMetazoa" id="ACON002095-RA">
        <property type="protein sequence ID" value="ACON002095-PA"/>
        <property type="gene ID" value="ACON002095"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_008926"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002095"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037377"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003707">
        <property type="term" value="F:nuclear steroid receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06956">
        <property type="entry name" value="NR_DBD_RXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06943">
        <property type="entry name" value="NR_LBD_RXR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000005">
        <property type="entry name" value="Retinoic acid receptor RXR-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.565.10:FF:000047">
        <property type="entry name" value="Ultraspiracle, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.565.10">
        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035500">
        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000536">
        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050274">
        <property type="entry name" value="Nuclear_hormone_rcpt_NR2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001723">
        <property type="entry name" value="Nuclear_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000003">
        <property type="entry name" value="Retinoid-X_rcpt/HNF4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001628">
        <property type="entry name" value="Znf_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24083">
        <property type="entry name" value="NUCLEAR HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00104">
        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00105">
        <property type="entry name" value="zf-C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00545">
        <property type="entry name" value="RETINOIDXR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00398">
        <property type="entry name" value="STRDHORMONER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00047">
        <property type="entry name" value="STROIDFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00430">
        <property type="entry name" value="HOLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00399">
        <property type="entry name" value="ZnF_C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48508">
        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51843">
        <property type="entry name" value="NR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00031">
        <property type="entry name" value="NUCLEAR_REC_DBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51030">
        <property type="entry name" value="NUCLEAR_REC_DBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 12" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Nuclear receptor" evidence="14">
        <location>
            <begin position="131"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="NR LBD" evidence="15">
        <location>
            <begin position="219"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="83"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="83"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="111"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00078913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002095-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="483" mass="53521" checksum="E28B10F302E841BB" modified="2020-08-12" version="1">MDTGDRGFDAALVGHMGPLSPPDLKPDLKPDISLLNGSGGGPFSPGNNCGPGSPGSGGFNSVVLQQQNALVVGGALGNGTGQQNLLVGGGSQQQQQQQQQGSNGGGGGSGPNTPSNITQQYPPNHPLSGSKHLCSICGDRASGKHYGVYSCEGCKGFFKRTVRKDLSYACREDKNCTIDKRQRNRCQYCRYQKCLACGMKREAVQEERQRSSKFSMKSEEINSTSSVRDVVVDRFLEAEQIGEQKSGDNAIPYLRVGQNSMIPSEYKGAVSHLCQMVNKQIYQLIEFARRLPNFSNLPREDQVTLLRSGWNEMLIASVAWRSMEYIETERPPDGRNDGRVTIRQPQLMCLGPNFTLHRNSAQQAGVDSLFDRILCELAIKMKRLDVNRAELGILKAIILFNSDIRGLKCRKEIDQMREKIYACLDEYCKTQHPSEDGRFAQLLLRLPALRSISLKCIDHLNFLRLLGDKQLDNFIIEMLDTPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V1C0</accession>
    <name>A0A6E8V1C0_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="5">BTB domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8V1C0"/>
    <dbReference type="EnsemblMetazoa" id="ACON000080-RE">
        <property type="protein sequence ID" value="ACON000080-PE"/>
        <property type="gene ID" value="ACON000080"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_003472"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000080"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032448"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048468">
        <property type="term" value="P:cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003006">
        <property type="term" value="P:developmental process involved in reproduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18315">
        <property type="entry name" value="BTB_POZ_BAB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000138">
        <property type="entry name" value="Fruitless, isoform N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051095">
        <property type="entry name" value="Dros_DevTransReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110">
        <property type="entry name" value="BTB DOMAIN TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110:SF107">
        <property type="entry name" value="SEX DETERMINATION PROTEIN FRUITLESS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00614">
        <property type="entry name" value="ZnF_BED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="4">
        <location>
            <begin position="30"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="112"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="300"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="439"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="117"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="146"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="182"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="198"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="233"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="244"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="309"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="332"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="449"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000080-PE"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="593" mass="65932" checksum="0480C48EEE978B7E" modified="2020-08-12" version="1">MDQQYCLRWNNHQSNLTTVLTTLLQDEKLCDVTLACEKGMVKAHQAILSACSPYFEQIFVENKHPHPIIYLRDVEVNEMRALLDFMYQGEVNVGQHNLQNFLKTAESLKVRGLTESSADRYSADTDSKLRSERIRDSRDERDSLPNASSNNSNNNNNSSSNNNNNNNTISSNNNNNSLHHGPLRDKELTEHEQLERLQQQQQQTHHQQQQHPSSHQQQSQQHPSSQHQQPTHQTHHHHHHHQHGGGVGGGGGGGGGGGGGGGSAGPVQQPSRSASIDLMQSALVDERDYLAAEDREISTVENKKKRKMSTTCDNSSPSTPSLMNERQGGYESQASSHSSFKQSPKPEDEFKVSSPAPVHPLAGHPLSGIKQELPELPVRHSLSSELMQPLKMPLYADEMSASIGLEPHAHLNHLRHKSKHPIPINMNADDMNNILAPGNMGSLNESGDSDAHLSHPDHPDNIDGPGGGGGGSRYEHHLSRHASSILPSSLVSSPDGTDLPHHTHYQLHHQMSYHNMFTPSREPGTAWRCRSCGKEVTNRWHHFHSHTPQRSLCPYCPASYSRIDTLRSHLRIKHADRLNAPKFSNPPNCKLPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V1C6</accession>
    <name>A0A6E8V1C6_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Cytochrome b5 heme-binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11 12">Belongs to the cytochrome b5 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040225429.2">
        <property type="nucleotide sequence ID" value="XM_040369495.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8V1C6"/>
    <dbReference type="EnsemblMetazoa" id="ACON002113-RB">
        <property type="protein sequence ID" value="ACON002113-PB"/>
        <property type="gene ID" value="ACON002113"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON002113-RC">
        <property type="protein sequence ID" value="ACON002113-PC"/>
        <property type="gene ID" value="ACON002113"/>
    </dbReference>
    <dbReference type="GeneID" id="120951019"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000011"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002113"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038366"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.120.10:FF:000002">
        <property type="entry name" value="Cytochrome b5 type B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018506">
        <property type="entry name" value="Cyt_B5_heme-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050668">
        <property type="entry name" value="Cytochrome_b5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19359">
        <property type="entry name" value="CYTOCHROME B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19359:SF129">
        <property type="entry name" value="CYTOCHROME B5 ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00363">
        <property type="entry name" value="CYTOCHROMEB5"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00191">
        <property type="entry name" value="CYTOCHROME_B5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3 12" id="KW-0349">Heme</keyword>
    <keyword evidence="9 12" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="13">
        <location>
            <begin position="6"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002113-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="115" mass="12696" checksum="23551CD0B386E3C6" modified="2020-08-12" version="1">MAPTELQQYSLAQVAEHNKPNDVWMVIHDKVYDVTKFLAEHPGGEEVLIEFAGKEATADFDDVGHSTDAKEQMKQFLVGEILEAERKKKVTTGQTDPSNAAASSWFGSIKAKLFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V3B2</accession>
    <name>A0A6E8V3B2_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Gustatory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Gustatory receptor which mediates acceptance or avoidance behavior, depending on its substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the insect chemoreceptor superfamily. Gustatory receptor (GR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8V3B2"/>
    <dbReference type="EnsemblMetazoa" id="ACON001117-RG">
        <property type="protein sequence ID" value="ACON001117-PG"/>
        <property type="gene ID" value="ACON001117"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_003504"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_013148"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001117"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_036433"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042470"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007635">
        <property type="term" value="P:chemosensory behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008049">
        <property type="term" value="P:male courtship behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050909">
        <property type="term" value="P:sensory perception of taste"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013604">
        <property type="entry name" value="7TM_chemorcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21143:SF104">
        <property type="entry name" value="GUSTATORY RECEPTOR 8A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21143">
        <property type="entry name" value="INVERTEBRATE GUSTATORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08395">
        <property type="entry name" value="7tm_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="41"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="83"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="145"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="183"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="298"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="338"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001117-PG"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="383" mass="43421" checksum="A2E0DF6E80244240" modified="2020-08-12" version="1">MLTSNEDALKAFECFTLVVKCLGLYPPVTCSRKRTLLGYKLCNLLAIAFSATFYGFLLLFHSLDLETIKLGSGSLITSRMHDVYFVSRYLTVLAVLLHSYINQNSVDQLFGSLNEVSSALVTLNTNHASEGSIGYFGTQRFARHLRWLMLVYPLMFVSMTGFVERWLPRFGVQVHAFNLVRFLYFYSYVHLWAQAILTLFLVLSRYIALNNLFRMYFPTSPSAVGNPSEFAICSEKEQINVLKQIKILHDKLNDVVELVNYCFSVQGKRIGILVHKAINCSSSSAVINEVQCCKGANMMLPVCNVPCAWIILILYLSRQLNLFSQQLLHRSPVITCGLFVYDWTLWYTMIGATATYLIILIQFDVSFPNLVNVNATAVYRGST</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V3D0</accession>
    <name>A0A6E8V3D0_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="7">SH3 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8V3D0"/>
    <dbReference type="EnsemblMetazoa" id="ACON001148-RB">
        <property type="protein sequence ID" value="ACON001148-PB"/>
        <property type="gene ID" value="ACON001148"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012943"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001148"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035588"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051666">
        <property type="term" value="P:actin cortical patch localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097320">
        <property type="term" value="P:plasma membrane tubulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00174">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046982">
        <property type="entry name" value="BIN3/RVS161-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47174">
        <property type="entry name" value="BRIDGING INTEGRATOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47174:SF3">
        <property type="entry name" value="BRIDGING INTEGRATOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00499">
        <property type="entry name" value="P67PHOX"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="6">
        <location>
            <begin position="430"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="6">
        <location>
            <begin position="511"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="35"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="248"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="328"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="36"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="253"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="274"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="348"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="412"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001148-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="585" mass="61641" checksum="C59F2DAAB76FB356" modified="2020-08-12" version="1">MSRNMTIPSRPAPPPPQRNTSAALRYTPTTDWDFCAFGTTANAGASGTASTGQTSVGSGDYFQPQHQQNVKAKKPPPPRPPPPKLPTSVPTAPLKKPGQPQSINILSSLFGHKRPSNGAAAPNKPTGSAVPHQKHGFSSGSVKLLPPPPSANMAKHGGKGSNYAGGNENVQHIGANYGSLLPPPNVGSGSGASAEMQLISFDSPPSSPTFTQKSCSDCISVDSFSSDSNYSSPNNGSMSQAESGFEDDFVACGNSNRPRASSSVPWGDLDPFEGGSTAGTSSGRAKTAPPPVIYGTIPLASSSSTAQIRTPLINSKLQKSDFVDPLCNGQSVLPRHPTMSMPTIIKPPTSSNTSDSQKSTPSHSVMPRHAAASGSSKQMAPIPPTAGQDPYAGMEADSSSDVLGAFDSLPSQPMPSIPPPPPPPATEAYAEDPYGIALYDFQGEADEDLSFKANEKIYLLKRLTEEWYMGRDKRGLEGMFPVNYVEVKVPLVDEQSNESVTLLIPASSDNGGGVKVRALYNFKAEAPEDLTIMENDVVAVLYQITPEWLYGEINGRRGQFPANYIEFVPPNLPQMQAVPGSNSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V3E4</accession>
    <name>A0A6E8V3E4_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Gustatory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Gustatory receptor which mediates acceptance or avoidance behavior, depending on its substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the insect chemoreceptor superfamily. Gustatory receptor (GR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8V3E4"/>
    <dbReference type="EnsemblMetazoa" id="ACON001169-RB">
        <property type="protein sequence ID" value="ACON001169-PB"/>
        <property type="gene ID" value="ACON001169"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009279"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001169"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_029245"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007635">
        <property type="term" value="P:chemosensory behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008049">
        <property type="term" value="P:male courtship behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050909">
        <property type="term" value="P:sensory perception of taste"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013604">
        <property type="entry name" value="7TM_chemorcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21143:SF134">
        <property type="entry name" value="GUSTATORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21143">
        <property type="entry name" value="INVERTEBRATE GUSTATORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08395">
        <property type="entry name" value="7tm_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="59"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="89"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="124"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="217"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="376"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001169-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="401" mass="46295" checksum="5FD6E9D7EE2F4616" modified="2020-08-12" version="1">MQNSSRMMSFINYPRSICNQLNHSRTQCDSAAADRASTTLGRALVWWFTMSHDVAITKAVLVLIKCFGQLYGILPLASNAFTFERSRPVLWYSRTIALLLMVIYPCAYIVLLTDHEFPILALSYYIIMLQFTLNYIIVGVLYLHVLFDPTPLLHVLNELIERTRALLRHIDPPAGESPVGRRLFFKTIAIDLLQSFTWYGTFMWLQRKFGATYILAFWLNSIAVMQIVAALNILLATLLWGAFLYRLINERVRTVIGSLLQLDDTDRQVRDSATGRERRFICNHIYLQLTILRTHHSALTKCIQNIVEFYNTPLALIMLYQFLIIISEAEKTGVLLNEFLPTDPNPRVERCIESFAIELLHEDFKINNCGMYDVDYTLMFSMIATITSYLIMLVQFQLAEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V3G7</accession>
    <name>A0A6E8V3G7_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ras-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="EnsemblMetazoa" id="ACON003140-RA">
        <property type="protein sequence ID" value="ACON003140-PA"/>
        <property type="gene ID" value="ACON003140"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010720"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003140"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038815"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13313">
        <property type="entry name" value="PH_NF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05130">
        <property type="entry name" value="RasGAP_Neurofibromin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.506.10:FF:000014">
        <property type="entry name" value="Neurofibromin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.506.10:FF:000015">
        <property type="entry name" value="Neurofibromin isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.506.10">
        <property type="entry name" value="GTPase Activation - p120gap, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054071">
        <property type="entry name" value="PH_NF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039360">
        <property type="entry name" value="Ras_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001936">
        <property type="entry name" value="RasGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10194:SF142">
        <property type="entry name" value="NEUROFIBROMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10194">
        <property type="entry name" value="RAS GTPASE-ACTIVATING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13716">
        <property type="entry name" value="CRAL_TRIO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21877">
        <property type="entry name" value="PH_NF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00616">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00323">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50018">
        <property type="entry name" value="RAS_GTPASE_ACTIV_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ras-GAP" evidence="4">
        <location>
            <begin position="1240"/>
            <end position="1436"/>
        </location>
    </feature>
    <feature type="domain" description="CRAL-TRIO" evidence="5">
        <location>
            <begin position="1572"/>
            <end position="1730"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="834"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="2455"/>
            <end position="2504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="2520"/>
            <end position="2542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="838"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="2466"/>
            <end position="2480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="2481"/>
            <end position="2500"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003140-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2758" mass="309908" checksum="944C22D2D33B7CA4" modified="2020-08-12" version="1">MATQKPGEWANSLLARFEEQLPYRTGPHGTQARLSIDETMNCLIQISRYRFSLVISGLTKMLQRVNEIVSIGPTSPPERCCYDSLIIILETLERCLSGQSKDTARFEEAMNVKLLLREICQFIDIQNENNQNATSLKALASKVLFALSQNHFGAVFNRISARLQELSTCAEENPDYSDIELIQHIDLDVHRLTKLLTETIQKFKSLKKSAHMILLSSLERALWNWIEFHPKEFEDLQRNPNDELSKCCETFFDILDSYSENKKARAAVWPLQIMLLILSPKVLEEIVNADSGAPCSPRHLKKKHFMEGIKKGLGAHASSKQSTESAAIACVKLCKASTYININDSNNVTFQLVQSVINDLKALLFNPAKPFSRGQGFNFQDIDLMIDCWVSCFRIKPHNNEALKVCLSLNSPPAYHFVIVSSLLKIVTQARLPWWPQIDLVYARSGELRGLFTDTLNKATQGYIAHTPLRMITSLTLKSKDAQSRLTRPDEGPAHKALLLLMVRLIHADPMLLLNSLGKAGHEVQSSTLELINGLVSLVHQPTMPDVAQEAMEALLALHSPDKIEVWNPEAPINTFWDVSSQVLFSISQKLIQHQIANYTDVLKWLREILICRNTFLQRHKDYANVGSQIAICRQAHIKLEVVFFMYLWSVDLDAVMVSLSCFGLLCEEAEIRSGSDELTVGFILPNYHLYQELSHTSATLTSPQNAESRYSFFEHLHGRVTLQRNIMSLLRKIEHCVNGVQPAWEETFRNWEVTSKLLQSYPKGKPEEGQAEVFHRSMGKRRASHQSSEHDLEEQITEWANMTWFLLALGGVCLQKPRNQRQATQGYNLPIGTAGPSLMQSTTSLSSSSSGRGSMHPIMGSLVSSIGPGSSQEVQYCPVTQFIGQLLRLLVCNNEKFGPQIQKHVKELVGQEMSAQLYPILFDQIRSIVEKFFDQQGQVVVTDINTQFIEHTIYIMKSVLDGRQSKDQNDQPANAEHLGVTSIENLMLAIVRYVRHLDMTVHAIHIKTKLCQLVEVMMKRRDDLAFRQEMKFRNKLVEYLTDWVMGTSHQIAPPGSGDVTIITRDLDQACMEAVAALLRGLPLQPEESDRGDLMDAKSALFLKYFTLFMNLLNDCVDGSEADKDTNNPPLLPPRPRVAAGKLTALRNATIQAMSNLLSANIDSGLMHSIDLSYNPDLQTRAAFMEVLTQILQQGTEFDTLAESVMADRFEQLVQLVTMISDKGELPIAMALASVVTTSQMDELARVLVTLFDAKHLLSPLLWNMFYREVEVSDCMQTLFRGNSLGSKIMAFCFKIYGASYLQGLLEPLIRPLLGDEPTSSFEVDPARLEPTEDIEVNRKNLIALTQKVFDAIVNSADRFPPQLRSMCHCLYQVLSKRFPNLLQNNIGAVGTVIFLRFINPAIVSPQELGIVGKQVPTQIKRGLMLMSKILQNIANHVEFSKEQHMLCFNDFLRAHFEAGRRFFIQIASDCETVDQTSHSMSFISDANVLALHRLLWSHQERIGDYLSSSRDHKAVGRRPFDKMATLLAYLGPPEHKPVDSHLLFSSYARWSSIDMSSTNFEEIMVKHQMHEKEEFKTLKSMNIFYQAGTSKAGNPVFYYIARRYKIGETNGDLLIYHVILTLKPFCHSPFEVVIDFTHTCSDNRFRTEFLQKWFYVLPEVAYENLYAAYIYNCNSWVREYTKFHDRILAPLKGCRKLIFLDSPAKLNDVIDPEQQKLPGATLSLDEDLKVFNNALKLSHKDTKVAIKVGPTALQITSAEKTKVLAHSVLLNDVYYASEIEEVCLVDDNQFTLSIANESSQLSFIHNDCDNIVQAIIHIRNRWELSQPDSVTVHQKIRPKDVPGTLLNMALLNLGSSDPNLRTAAYNQLCALTATFDLKIEGQLLETQGLCIPSNNTIFIKSVSETLATNEPHLTLEFLEECIQGFQRSTIELKHLCLEYMTPWLANLVRFCKPSDEGKRQKQVAQILEKLINLTIEQKEMYPSIQAKIWGSIGQIPELIDMVLDNFIHKSVSSGLGSPQVEIMADTAVALASANVQLVAKKVIGRLCRVMDKTCHSPTQYLEQHMMWDDIAILARYLLMLSFNNCLDVARHLPYLFHTVTFLVCTGSLSMRASTHGLVINIIHSLCTCTKPSFSEETQRMLRLSLDEFSLPKFYLLFGISKVKSAAVTAFRSSCRHPNDRWLGNERVSQAPPADRERLALPSLEVITEALLEIMEACMRDIPDCDWLQTWTSLAKSFAFCFNPALQPRALIVFGCISKSVTDQDVKQLLRILVKALESFNDIILLEALVMCLTRLQPLLRPESPIHRALFWVAVSVLQLDESTLYAAGLALLEQNLHTLNSQQLFDNQNIADVMMATREPLEWHFKQLDHAVGLSFKSNFHFALVGHLLKGFRHPTPTTVSRTSRVLTMLLGIVAKPHRRDKFEVTPDSVAYLTALVCFSEEVRSRCHVKHTVPRWPVESGGSGDSASGSSSDPSAPNSAGGGPLTGGSGPGGSSGSGGHSVRRQKSWDMLDQSAIQYARQSHKVQQHQERGSRSSVSNESNVLLDPEVLPDSSTQALVLTVLATLVKYTTDEAETRVLYQYLAEGSIVFPKVFPVIHSLLDQKVNNVLSVSNDQIVLASVQSIIQNMLASEDASQQPLHFLQSCGFGGLWRFAGPFTKYNMMVESSELFVNFLEAMVETCLPVEESTPMPPSPRPYNLSSSLSSLTLGSPTDKAFSSESLDHDGFSGSVSSLRRASCSKARTGSKHRFIDSPTHNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V3G8</accession>
    <name>A0A6E8V3G8_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Small acidic protein-like domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ACON001208-PA"/>
        <property type="gene ID" value="ACON001208"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000209"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001208"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038401"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="26"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001208-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="82" mass="9084" checksum="80FB931C729C4E2B" modified="2020-08-12" version="1">MDFLRNYEAADQDESEDMDSNREHTASPTGSSSSSASAGSPKRSTADKQPSARDHKDKKQDAKESKGQHKSRKERDENTSII</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V5H9</accession>
    <name>A0A6E8V5H9_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Guanylate kinase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MAGUK family.</text>
    </comment>
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    <dbReference type="EnsemblMetazoa" id="ACON002711-RC">
        <property type="protein sequence ID" value="ACON002711-PC"/>
        <property type="gene ID" value="ACON002711"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000007"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002711"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032683"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030054">
        <property type="term" value="C:cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="GMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06798">
        <property type="entry name" value="PDZ_MPP5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12036">
        <property type="entry name" value="SH3_MPP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000088">
        <property type="entry name" value="MAGUK p55 subfamily member 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000469">
        <property type="entry name" value="MAGUK p55 subfamily member 5"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008144">
        <property type="entry name" value="Guanylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020590">
        <property type="entry name" value="Guanylate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014775">
        <property type="entry name" value="L27_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004172">
        <property type="entry name" value="L27_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050716">
        <property type="entry name" value="MAGUK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035601">
        <property type="entry name" value="MPP5_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
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        <property type="entry name" value="PDZ_sf"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR23122">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02828">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00856">
        <property type="entry name" value="GUANYLATE_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="L27" evidence="9">
        <location>
            <begin position="514"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="8">
        <location>
            <begin position="659"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="6">
        <location>
            <begin position="751"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate kinase-like" evidence="7">
        <location>
            <begin position="901"/>
            <end position="1081"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="64"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="182"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="296"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="77"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="108"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="182"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="192"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="233"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="296"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="334"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007014"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002711-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1097" mass="119926" checksum="9FAAF76162A9C3D0" modified="2020-08-12" version="1">MDTGRPRRRSGSSIVVLGGDDTLKPSIPIDDYQEDLEMYNMLAANQDNGPHREMAVDVPESFIARNKTPPRYPPPRSTVTAASAAASTPATPASTQVNHVSNAMPQVAKHHHHHNHHHQQQQHPNHHLHHHHPPSSVHHNTHHHHGGGGVSLELEPLDSYSERHLSSLDSSSQEYVKKGIISGGGVGGGGSSKAPSSLGSVTSEFEPSLNGFGPLHGKHHHHSTVGGGGGDLLRGSLSTNRSPSPSSSGDLGPPEYDVGPHRELPVDVPDSFVEMVKGPPRYPPPKPLIIKDAIRKKESCSSSESIDKPKPQTQSPSPPARNELNGSSKPVPPPRDHLRIEKDGRLTNRAPVPAPQVPDRKIVPNASSQHQHIGQVLEPTPDQLDSIKKFQEQLRRRREDEERIAAQNDFLRNSLRGSQRLRALQDNPIEKPPVGVDNEAYADDEVVAVAAEKIIGYGELVAALQRLQGHLNKHGLAALAGRVTAAQSLLLGPGIARALAVRTAVLERRRPKIPNPICPNAQALAKDCVESLAQSRSQTAIELCDLLSTYEMEGLLLAHDRIASTTDRSPIASYGGSPITVPVPLGTASNNTTSNNTTTSNNSINNNTLTSAVKPATVMPSIPNNASLVNNNNNNIAKREPMSVPLGVLRDGSQDHIKIIQIEKSSEPLGATIRNEGEAVVIGRVVRGGAAEKSGLLHEGDEILEVNGIEMRGKSVNDVCALLGSMTGTLTFLVVPAGVPQILPGLGMRDPPVLHVRAHFDYDPEDDLYIPCRELGISFQKGDVLHVISRDDPNWWQAYREGEEDQTLAGLIPSQSFQHQRESMKLAIAGEVGLRTRKDINGKAGGGSTLLCARKGRKKKKKASSEHGYPLYATATADDPDPEEILTYEEVALYYPRASHKRPIVLIGPPNIGRHELRQRLMADSERFAAAIPHTSRAQREGEVPGQDYHFISRQQFESDILARKFVEHGEYEKAYYGTSLEAIRAVVASGKICVLNLHPQSLKLLRSSDLKPYTVLVAPPSLEKLRQKRIRTGEPYKEEELKEIIATARDMEARWGHLFDMIIINNDTERAYHQLLAEINSLEREPQWVPSSWLHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V9N5</accession>
    <name>A0A6E8V9N5_ANOCL</name>
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        <recommendedName>
            <fullName evidence="10">Ras-specific guanine nucleotide-releasing factor 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="EnsemblMetazoa" id="ACON001238-RA">
        <property type="protein sequence ID" value="ACON001238-PA"/>
        <property type="gene ID" value="ACON001238"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002097"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_003977"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001238"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033748"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037474"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13261">
        <property type="entry name" value="PH_RasGRF1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00155">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06224">
        <property type="entry name" value="REM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.840.10">
        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.870.10">
        <property type="entry name" value="Son of sevenless (SoS) protein Chain: S domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008937">
        <property type="entry name" value="Ras-like_GEF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000651">
        <property type="entry name" value="Ras-like_Gua-exchang_fac_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019804">
        <property type="entry name" value="Ras_G-nucl-exch_fac_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023578">
        <property type="entry name" value="Ras_GEF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001895">
        <property type="entry name" value="RASGEF_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036964">
        <property type="entry name" value="RASGEF_cat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113:SF99">
        <property type="entry name" value="RASGEF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00617">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00618">
        <property type="entry name" value="RasGEF_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00147">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00229">
        <property type="entry name" value="RasGEFN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48366">
        <property type="entry name" value="Ras GEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00720">
        <property type="entry name" value="RASGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50009">
        <property type="entry name" value="RASGEF_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="5">
        <location>
            <begin position="22"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="DH" evidence="7">
        <location>
            <begin position="274"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GEF" evidence="6">
        <location>
            <begin position="1261"/>
            <end position="1493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="765"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1024"/>
            <end position="1089"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1146"/>
            <end position="1167"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="440"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="774"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="788"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="844"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="872"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="889"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="925"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1026"/>
            <end position="1037"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1063"/>
            <end position="1076"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1152"/>
            <end position="1167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001238-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1505" mass="168931" checksum="7B0D90D44BF3D519" modified="2020-08-12" version="1">MQRSVRVNENQLIMLSDRAQYDHSMHGYLHKRTSDNNKWQMRWFVLYQNLLFYYESEQSSRPSGLIFLEGCYCERLVSAPSISGPPISTSTGQAPKIIKEEKLQHCFTICYRRENQRQYELRAATESECSAWIIAIREASFNKLLLQKEELEQKHVHLLQVVESEKTAKWQYTQQCEELASEIRKLRAEICVLRKETRSSYSATPVGRGSFSVPGDSQDDHGSLPGLCASVQDSIELRKIKKVQSFFRGWLCRRRWKQIVEEYIRSPHAESMRKRNHLVFSMVEAEEEYLEQLEVLVACFLRPFKMAASSKRPPCSHEDVNSIFLNSETVLFLHQIFLKGLTSRLESWPTLVLGDLFDMLLPMLSIYQEYVRNHHYSLQVLTECKSNTNFATVLKRLEAKPACQGRSLETFLTYPMHQIPRYIITLHELLAHTPHDHVERKSLQNARQQLEDLSRQMHDEVSETENLRKNLAVERMIVEGCDILLDVNQVFVRQGSLVQVPPGRGRIRSRLASFKSERDAVRQCFLFSNHMIIATRTSGGRLHLLPDVGKIPLADATLVEDPSEVQHDDEDASVCSASTRGSTLSVTESVNAGNRDFKIFVESKSGARHGIHLVAPTIQDKEAWISDISQCLDNIHMHSLLSPGIGGSSGGKTAAMAVRVSSLLAGHQALRADPRLFKDDVDIRFSRTLNSCKLPQVRYATPERLLQRLTDLRFLSIDFLNTFLLTYRVFTDGETVLNALKSVFYDPPVEPSCDCEHQTDFLELPYQDGRASPRRTSGASSVSETAMSGPSSPSNLSSVTLVGSTGSGGQDKSDDTPTEGTFKYGKAPTSPLPERVNPRAHKMATLATAAPPTPTICTTDTSQTEEEKPGQASRTPDKQQQPTLDHQQHSPQHQQQQIKTPPTPPTVTTTPCYSPANGQPPNGAPSPLHQPLQQQQSLPLKSEQATIVQGRLEILGPKIVTQPYLTTTTTTTVTITTTTTATSTKSISPPHLLPMDRRPSVGHNIAIPHAALCQHRHSLQLNGDGSLFSKSEKTSSSPRLTTRKFSAPKTPERQRKSLFGTPQRDRDSSSTRRFSESDDDMATSIFTTPRGSLGGVSLNTISSRASMQHDPVPHCSSKVGVVITSYRQSQRRSSTSTAAAAFAIATSASSNPRDEPPEVEARNRKESVVSSPATMRVLNVLRHWVSKHFQDFEQDAALRSQTIAFLDDITCSPNLLPTEHRAASQLLRLLCRDDIDSGKLMLEILLTPPQTPSKESIETLSALEIAEQMTYLDHQIFLAIRSEEFLGQAWMKSDKKSRAEHIILMTKRFNDGSRLVCSEIVSRSNMAARVAAIEKWTAVADICRCLHNFNGVLQICAAFTNAAVYRLKKTWDKVPRTIKSTITKLQAVVCSDGRFRVMREALHRCDPPCIPYLGMYLTDLSFIEEGTPDFTPDGLLNFSKMRMVAHVIREIRHFQQTPYKIDHIPKVTSYLLDTSLLLDDDELYHKSLQIEPRSSRLSAPNTANV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V9N6</accession>
    <name>A0A6E8V9N6_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Gustatory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Gustatory receptor which mediates acceptance or avoidance behavior, depending on its substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the insect chemoreceptor superfamily. Gustatory receptor (GR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8V9N6"/>
    <dbReference type="EnsemblMetazoa" id="ACON001117-RA">
        <property type="protein sequence ID" value="ACON001117-PA"/>
        <property type="gene ID" value="ACON001117"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_003504"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001117"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_036433"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007635">
        <property type="term" value="P:chemosensory behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008049">
        <property type="term" value="P:male courtship behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050909">
        <property type="term" value="P:sensory perception of taste"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013604">
        <property type="entry name" value="7TM_chemorcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21143:SF104">
        <property type="entry name" value="GUSTATORY RECEPTOR 8A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21143">
        <property type="entry name" value="INVERTEBRATE GUSTATORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08395">
        <property type="entry name" value="7tm_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="58"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="95"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="144"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="184"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="304"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="344"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001117-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="389" mass="44958" checksum="D208D8EA7B18E6BC" modified="2020-08-12" version="1">MYRRSVAVVMLLNESLNVYSSMEVLYWILRLFGYAPFRLQLDADTPDRDAFGTWRTKLYTISFGLVYTLAFIKFMSELKHTGFSASMIEGKGERMYFTFNFFTTMYGVVNGYAVRDKIEKMLYKLHAVDQKISQWKRNVDHRRFYRNVWAGVYLIAIVLLIYIYGTIITCQLHESSCNMQILFVFIYLLFVNSYALMMLQCTAFMEIIRTRYKLINGCFRMYFPTSPSAVGNPSEFAICSEKEQINVLKQIKILHDKLNDVVELVNYCFSVQGKRIGILVHKAINCSSSSAVINEVQCCKGANMMLPVCNVPCAWIILILYLSRQLNLFSQQLLHRSPVITCGLFVYDWTLWYTMIGATATYLIILIQFDVSFPNLVNVNATAVYRGST</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V9N8</accession>
    <name>A0A6E8V9N8_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Gustatory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Gustatory receptor which mediates acceptance or avoidance behavior, depending on its substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the insect chemoreceptor superfamily. Gustatory receptor (GR) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8V9N8"/>
    <dbReference type="EnsemblMetazoa" id="ACON001117-RF">
        <property type="protein sequence ID" value="ACON001117-PF"/>
        <property type="gene ID" value="ACON001117"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_003504"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001117"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_036433"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007635">
        <property type="term" value="P:chemosensory behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008049">
        <property type="term" value="P:male courtship behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050909">
        <property type="term" value="P:sensory perception of taste"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013604">
        <property type="entry name" value="7TM_chemorcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21143:SF104">
        <property type="entry name" value="GUSTATORY RECEPTOR 8A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21143">
        <property type="entry name" value="INVERTEBRATE GUSTATORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08395">
        <property type="entry name" value="7tm_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="42"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="73"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="130"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="173"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="290"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="330"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001117-PF"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="375" mass="42615" checksum="4720171E3CC28217" modified="2020-08-12" version="1">MRWFSVNSVYETVQPFMFLLGCVGLAPFGYRLGMKPVNRYLEFCYVLVYGGMYTYALYSFLFVANVTDFHLSLIIGTIECINLSCQYLTMVFAIVFAWIVKHRISFILQTLHECDVQLSKFSSPIDHRKLHLKVSMLAVGIVTSYMLLLAIHLPLILELVPHIEPSLKEILPSAMFGLCFLLQICQFLLFLLVLHDRYCAINQAFRMYFPTSPSAVGNPSEFAICSEKEQINVLKQIKILHDKLNDVVELVNYCFSVQGKRIGILVHKAINCSSSSAVINEVQCCKGANMMLPVCNVPCAWIILILYLSRQLNLFSQQLLHRSPVITCGLFVYDWTLWYTMIGATATYLIILIQFDVSFPNLVNVNATAVYRGST</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V9Q7</accession>
    <name>A0A6E8V9Q7_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Inwardly rectifying k+ channel</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8V9Q7"/>
    <dbReference type="EnsemblMetazoa" id="ACON001283-RA">
        <property type="protein sequence ID" value="ACON001283-PA"/>
        <property type="gene ID" value="ACON001283"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005523"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001283"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034117"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034702">
        <property type="term" value="C:monoatomic ion channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005242">
        <property type="term" value="F:inward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034765">
        <property type="term" value="P:regulation of monoatomic ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1400:FF:000001">
        <property type="entry name" value="G protein-activated inward rectifier potassium channel 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000078">
        <property type="entry name" value="Putative Inward rectifier potassium channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1400">
        <property type="entry name" value="G protein-activated inward rectifier potassium channel 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041647">
        <property type="entry name" value="IRK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016449">
        <property type="entry name" value="K_chnl_inward-rec_Kir"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013518">
        <property type="entry name" value="K_chnl_inward-rec_Kir_cyto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040445">
        <property type="entry name" value="Kir_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11767">
        <property type="entry name" value="INWARD RECTIFIER POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11767:SF113">
        <property type="entry name" value="INWARDLY RECTIFYING POTASSIUM CHANNEL 2, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01007">
        <property type="entry name" value="IRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17655">
        <property type="entry name" value="IRK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01320">
        <property type="entry name" value="KIRCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0630">Potassium</keyword>
    <keyword evidence="3 12" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 12" id="KW-0813">Transport</keyword>
    <keyword evidence="5 12" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="123"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="166"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="206"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel inwardly rectifying transmembrane" evidence="15">
        <location>
            <begin position="89"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="Inward rectifier potassium channel C-terminal" evidence="16">
        <location>
            <begin position="241"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF17655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001283-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="19">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="436" mass="49956" checksum="66189B97124A33FD" modified="2020-08-12" version="1">MNYDSDQQQQQQQQQSLSLAPDGTGRWDRSMLKLGPFDPPHSPGGYGEQFGYSSPITPVTPNTPLVCYPMKNRVLRPGVNRKYRKRAILKNGDCNVVLSRPPRQHLRFLQDIFTTLVDAQWRWTLLVFAFSFVGSWLFFAVIWWLISYTHGDLEELHLPDNQSEIGWIPCVYNIYSFTSAFLFSIETQHTIGYGVRTTTEECPEAIFVMCFQSIYGLMIQAFMVGIVFAKMTRPKQRSQTLLFSKNAVVCQRDGELCLMFRVGDMRKSHIIGASVRAQLIRTKTTREGETMAQYQHELDVGSDGSSSELFFIWPQIVVHRIDKNSALYNMSASDMLRERFEVVVILEGTVESTGQSTQARSSYVNTEILWGHRFEPVVCYNREQQGYEINYSKFDSTLQVDTPLCSARELAEFYRAQDDYRPPTGKDSAITDCVSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V9T4</accession>
    <name>A0A6E8V9T4_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ig-like domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_049461345.1">
        <property type="nucleotide sequence ID" value="XM_049605388.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8V9T4"/>
    <dbReference type="EnsemblMetazoa" id="ACON001201-RB">
        <property type="protein sequence ID" value="ACON001201-PB"/>
        <property type="gene ID" value="ACON001201"/>
    </dbReference>
    <dbReference type="GeneID" id="120951972"/>
    <dbReference type="CTD" id="2768865"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010379"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001201"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030307"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032589">
        <property type="term" value="C:neuron projection membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050808">
        <property type="term" value="P:synapse organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000129">
        <property type="entry name" value="CLUMA_CG018772, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001705">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037448">
        <property type="entry name" value="Zig-8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23279:SF6">
        <property type="entry name" value="DEFECTIVE PROBOSCIS EXTENSION RESPONSE 7, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23279">
        <property type="entry name" value="DEFECTIVE PROBOSCIS EXTENSION RESPONSE DPR -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="151"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="241"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001201-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="468" mass="50201" checksum="BF11534E3B33764F" modified="2020-08-12" version="1">MVPMMALKNNRSRGRRREAGVRGNRCGCGGIASDCNPFISTPSAVFSRTLLVTFLLMTFNVNVCQVVEAAASASASAAAGIERRPSGDLQRPDGLKMHHHGIASAASPGSLQHATAGTSTASSSGSASAAALVAGGLASPSSSLTGPAEKPYFDDVNSRNVTTVVDDTAVLKCRVKHKGDRTVSWMRKRDLHILTSNIYTYTGDQRFSVIHPPDSDDWDLKIEYAQQKDSGIYECQVNTEPKINLAVYLDVTDFLSTAARAKIIGSQEVHVKKGSTISLSCVVNVHASSISWYHGSSIVDFDSARGGISLETEKTEGGTSSRLMLTRATLRDSGNYTCVPTGAISASVQVHVLNGEHPAAMQTSSGVPCPTHQTLILLFVSLNSCNVSKLIFFISNLLETMRFLLTTTIAHFLLHLRRLPSTLGAGRTKSTRFVLLTLATIGTRLRRTALQQRQHPAASPCSVRTQSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VBD4</accession>
    <name>A0A6E8VBD4_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Gustatory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Gustatory receptor which mediates acceptance or avoidance behavior, depending on its substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the insect chemoreceptor superfamily. Gustatory receptor (GR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040223604.2">
        <property type="nucleotide sequence ID" value="XM_040367670.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VBD4"/>
    <dbReference type="EnsemblMetazoa" id="ACON002635-RB">
        <property type="protein sequence ID" value="ACON002635-PB"/>
        <property type="gene ID" value="ACON002635"/>
    </dbReference>
    <dbReference type="GeneID" id="120949989"/>
    <dbReference type="KEGG" id="acoz:120949989"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004902"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002635"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031806"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007635">
        <property type="term" value="P:chemosensory behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008049">
        <property type="term" value="P:male courtship behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050909">
        <property type="term" value="P:sensory perception of taste"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013604">
        <property type="entry name" value="7TM_chemorcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21143:SF134">
        <property type="entry name" value="GUSTATORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21143">
        <property type="entry name" value="INVERTEBRATE GUSTATORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08395">
        <property type="entry name" value="7tm_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="7 8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026014947" description="Gustatory receptor" evidence="9">
        <location>
            <begin position="19"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="125"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="155"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="237"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="279"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="354"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002635-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="399" mass="45495" checksum="AAE7D4D02B864210" modified="2020-08-12" version="1">MLLLFSLYFNLLLSYAAGLVPFRYNFRKRRFDTCKPFVPVATILLLLFAAVQYRLGRLPERMKIPKVGMQDIIHAVENLVLNGNCVLIAVQQLLFLETYRQLLTDLLTGVRALARRTTVALGEQVQLLVQLVLLPCATYTLHVSVYITLIEWAEISIVQAVLFMVSLIPNIAHVNNFYALLFVQRLTLGEINDMLADLWTICIRPGANELLPPSSRLQLHLQQYQQCAGSVQKILPYYSLSVALYLLILFQELMSKFFYQFTHFYTLSLGNPSPHILEAMGTMMILVYGMYTAQLIAECYAISEKSQKIKSTVHRLSLIPGQDRYFHATINTFLLSLNHSMLKINIAGMFTMDFELLTGMLAAISNFVVLLMQFHIDYSKSKISYWHGNNSSKPLTVRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VBD7</accession>
    <name>A0A6E8VBD7_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="26">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="26">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="30 31">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="30" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="30">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20 26">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phosphocholine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38583"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:38584"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 25">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="22">Component of a P4-ATPase flippase complex which consists of a catalytic alpha subunit ATP8B1 and an accessory beta subunit TMEM30A. The flippase ATP8B1:TMEM30A complex can form an intermediate phosphoenzyme in vitro. Also interacts with beta subunit TMEM30B.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Apical cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cell projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="26">Membrane</location>
            <topology evidence="26">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 26">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="26"/>
    <dbReference type="EnsemblMetazoa" id="ACON002644-RB">
        <property type="protein sequence ID" value="ACON002644-PB"/>
        <property type="gene ID" value="ACON002644"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_003695"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002644"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039883"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140327">
        <property type="term" value="F:flippase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0005548">
        <property type="term" value="F:phospholipid transporter activity"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02073">
        <property type="entry name" value="P-type_ATPase_APLT_Dnf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000012">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="3.40.50.1000:FF:000497">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Phospholipid-transporting ATPase IC"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
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        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006539">
        <property type="entry name" value="P-type_ATPase_IV"/>
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    <dbReference type="InterPro" id="IPR032631">
        <property type="entry name" value="P-type_ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
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    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01652">
        <property type="entry name" value="ATPase-Plipid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF190">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF16209">
        <property type="entry name" value="PhoLip_ATPase_N"/>
        <property type="match status" value="1"/>
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    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 24 26" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-0966">Cell projection</keyword>
    <keyword evidence="12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="17" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="14 25 26" id="KW-0460">Magnesium</keyword>
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    <keyword evidence="10 25" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 24 26" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="9 26" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16 26" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
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            <begin position="273"/>
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    <feature type="transmembrane region" description="Helical" evidence="26">
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            <begin position="321"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
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            <begin position="1066"/>
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    <feature type="transmembrane region" description="Helical" evidence="26">
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            <begin position="1149"/>
            <end position="1168"/>
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    <feature type="transmembrane region" description="Helical" evidence="26">
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            <begin position="1180"/>
            <end position="1198"/>
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    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="1210"/>
            <end position="1233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="1253"/>
            <end position="1276"/>
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    </feature>
    <feature type="domain" description="P-type ATPase N-terminal" evidence="28">
        <location>
            <begin position="23"/>
            <end position="88"/>
        </location>
    </feature>
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        <location>
            <begin position="1035"/>
            <end position="1281"/>
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            <begin position="435"/>
            <end position="547"/>
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    <feature type="region of interest" description="Disordered" evidence="27">
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            <begin position="853"/>
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            <begin position="1350"/>
            <end position="1392"/>
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            <begin position="1427"/>
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            <begin position="437"/>
            <end position="446"/>
        </location>
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            <end position="513"/>
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            <begin position="514"/>
            <end position="525"/>
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            <begin position="532"/>
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            <begin position="877"/>
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            <begin position="1355"/>
            <end position="1364"/>
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        <location>
            <begin position="1365"/>
            <end position="1392"/>
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        </location>
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            <dbReference type="RuleBase" id="RU362033"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="Pfam" id="PF16212"/>
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    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
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    </evidence>
    <sequence length="1453" mass="162494" checksum="219A74F8F995B37E" modified="2020-08-12" version="1">MGETSKVQQQQQEKARLQNERRIRANDREYNTQFKYANNYIKTSKYSILTFLPLNLLEQFQRLANFYFLCLLILQLIPAISSLTPVTTAIPLIGVLMLTAIKDAYDDFVINNRRSKALRHGKLVDERWSGVQVGDIIRMDNDQFVAADILLLSSSEPNGLCFIETAELDGETNLKIKQCLPETAALGQQEDLLWKFNGEIVCEPPNNLLNKFEGTLTWKNQRYPLDNDKILLRGCIIRNTQWCYGVVIFAGKDTKLMQNSGKTKFKRTTIDRLLNFIIIGIVFFLLSICGFCTIASAIWEALVGYKFQIYLPWERIIPKDYLQGAISIGCLVFFSYAIVLNTVVPISLYVSVEVIRFAQSFLINWDEKMYYDKTKTHAKARTTTLNEELGQIQYIFSDKTGTLTQNIMTFNKCSIAGRAYGDVVDVRTGETVELSEPTFNSNTSLLLNAEEPPGRSTPQPTTTTTTTTITTEQPVQTPNKKHPPSLHVRFSQHSNSQETQTSISSTTTKQVTTPIPPTPPTPPQQPKTHIPAQSTTDVPPSGNNTQEGLHVMESVDFSFNPEYEPEFRWYDQGLLDAVRADEEHAHNFFRLLALCHTVMAEEKNGKLDYQAQSPDEAALVSAARNFGFVFKSRAPNSITIEVMGRTEEYELLSILDFNNVRKRMSVVLRRNNSIILYCKGADSVIYDRLGPNQHDLKARTQEHLNKFAGEGLRTLVLAERRLTKEFYESWLVRQREAALSLDGREDKLGAIYEEIECDMQLVGVTAIEDKLQDGVPQTIANLQLAGIKIWVLTGDKQETAINIGYSCQLLTDDMVDVFVIDGITKSEVEQQLRKYMDSLRIVNTYHPANVQKANQAHSQTVGGGGTTSETSAVTANGAHSSSSGPMVDIQNTSPPSVSVVTFRWDNRHKYTSIGGSEEAPDSVNCSNYSDGFEKGEPTLTDIDENTGVALVINGHSLVHCLTSELESKFLEIASHCKAVICCRVTPLQKAMVVELIKRAKNAVTLAIGDGANDVSMIKAAHIGVGISGQEGMQAVLASDYSIAQFKFLERLLLVHGRWSYYRMCKFLRYFFYKNFAFTLCHFWYAFFCGFSAQTVFDPMFISVYNLFYTSLPVLALGIFEQDVSDKSSVDYPKLYTPGMTNALFNTTEFIRSVLHGIFSSLILFLIPYGTYKDGISPDGYVLNDHMLLGSVVATILILDNTAQIALDTSYWTVFNHIMIWGSLLWYFFLDYFYNYVIGGPYVGSLTQAMKEATFWFTTVLTVIVLMIPVLASRFYFVDVFPSLPDKIRVQQRLALLRSRQSSDVLRTPSARKARRSLRSGYAFAHQEGFGRLITSGKIMRKLPQDFAFPLGLGSKKQQSTESTKNNNNNNNNNSSNNANNASNVVNGAGAVGTRTGTNTATATTLSAMAAVTTLTVNGNQQQQQQLVNLPGSSNGGDQHSPRAPCQDLDTINL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VFH1</accession>
    <name>A0A6E8VFH1_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">K Homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_040223097.1">
        <property type="nucleotide sequence ID" value="XM_040367163.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_049462732.1">
        <property type="nucleotide sequence ID" value="XM_049606775.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VFH1"/>
    <dbReference type="EnsemblMetazoa" id="ACON002896-RA">
        <property type="protein sequence ID" value="ACON002896-PA"/>
        <property type="gene ID" value="ACON002896"/>
    </dbReference>
    <dbReference type="GeneID" id="120949698"/>
    <dbReference type="CTD" id="124204813"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011137"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002896"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040328"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22435">
        <property type="entry name" value="KH-I_NOVA_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22436">
        <property type="entry name" value="KH-I_NOVA_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09031">
        <property type="entry name" value="KH-I_NOVA_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000022">
        <property type="entry name" value="RNA-binding protein Nova-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047275">
        <property type="entry name" value="KH-I_NOVA_rpt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047276">
        <property type="entry name" value="KH-I_NOVA_rpt2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047274">
        <property type="entry name" value="KH-I_NOVA_rpt3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10288">
        <property type="entry name" value="KH DOMAIN CONTAINING RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="8">
        <location>
            <begin position="23"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="8">
        <location>
            <begin position="116"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="8">
        <location>
            <begin position="435"/>
            <end position="508"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002896-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="538" mass="55697" checksum="02EDBB5DBFACA907" modified="2020-08-12" version="1">MELFEATGTTTATTTTATNGGGDNYHFKTLVPSVAAGAIIGKGGETIASLQKDAGARVKMSKAHDFYPGTTERICLISGTVEGILAVLDFITDKIREKPDITKALTEADAKQAQERDKQVKILVPNTTAGMIIGKAGAYIKQIKEESGSYVQISQKPKDLTLQERCITIIGEKENNRIACKMILAKIVEDPSSGTCLNVSYADINGPVANFNPTGSPFAASQNPNFSSSTASLNSAISGPLPNAAATGLLVNGTGLNLSLNLGAPSPQTNPTVTTQLLDHIKSAMRQSGYSDTATAEVHAALGVLAKYGVLGIGVGLQNGTHSTTLGYLGMPEMQQSSAAAAAAASAATGVYGAVGQLNLDSYLHGAGTATPRTTAIDRYDPTTFDPFRHPGTQAATPISINNNAFGLGNPSALNAAAQTLGSLSKSPTPAEMTTSKEKNVEIPEVIVGAILGPAGRSLVEIQHISGANIQISKKGIFAPGTRNRIVTITGQQNAINVAQYLIEERISQEETKRACQTAANAAATNKTSAASVATVTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VFH5</accession>
    <name>A0A6E8VFH5_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Chitin-binding type-2 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VFH5"/>
    <dbReference type="EnsemblMetazoa" id="ACON002909-RB">
        <property type="protein sequence ID" value="ACON002909-PB"/>
        <property type="gene ID" value="ACON002909"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006734"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002909"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_036930"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.140.10">
        <property type="entry name" value="Chitin binding domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002557">
        <property type="entry name" value="Chitin-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036508">
        <property type="entry name" value="Chitin-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051940">
        <property type="entry name" value="Chitin_bind-dev_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23301">
        <property type="entry name" value="CHITIN BINDING PERITROPHIN-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23301:SF108">
        <property type="entry name" value="OBSTRACTOR D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01607">
        <property type="entry name" value="CBM_14"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00494">
        <property type="entry name" value="ChtBD2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57625">
        <property type="entry name" value="Invertebrate chitin-binding proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50940">
        <property type="entry name" value="CHIT_BIND_II"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0147">Chitin-binding</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Chitin-binding type-2" evidence="7">
        <location>
            <begin position="19"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="7">
        <location>
            <begin position="89"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="7">
        <location>
            <begin position="146"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="203"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="203"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="233"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002909-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="250" mass="28086" checksum="CAF30526495CA069" modified="2020-08-12" version="1">MCHPPQHYRLASGQRQDQEDPCKTKSKVVGDVTYCDRYWECINNQPELYDCPNGLVFAGKHRGVTEGCDYPWRSNYCDGKQLANGPISTEHCDWLYGIFGHETSCTRYWTCWNGTATEQLCIGGLLYNENAHSCDWPENVDGCQKHPLCNEDANGNVPLGKSCNRYWQCQGGYPRLQRCPAMLVFDRRSLRCVVPPTEDCDVPTTPLPLEYEPEQQQQQQGKGNPNAIYQPSGAGGLQGGKLQSGGRNKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VFK0</accession>
    <name>A0A6E8VFK0_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the STXBP/unc-18/SEC1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VFK0"/>
    <dbReference type="EnsemblMetazoa" id="ACON003023-RB">
        <property type="protein sequence ID" value="ACON003023-PB"/>
        <property type="gene ID" value="ACON003023"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000758"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003023"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042088"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051650">
        <property type="term" value="P:establishment of vesicle localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.60:FF:000001">
        <property type="entry name" value="syntaxin-binding protein 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2060:FF:000001">
        <property type="entry name" value="syntaxin-binding protein 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.830.10:FF:000001">
        <property type="entry name" value="syntaxin-binding protein 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.830.10">
        <property type="entry name" value="Syntaxin Binding Protein 1, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043154">
        <property type="entry name" value="Sec-1-like_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043127">
        <property type="entry name" value="Sec-1-like_dom3a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001619">
        <property type="entry name" value="Sec1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027482">
        <property type="entry name" value="Sec1-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036045">
        <property type="entry name" value="Sec1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11679">
        <property type="entry name" value="VESICLE PROTEIN SORTING-ASSOCIATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00995">
        <property type="entry name" value="Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005715">
        <property type="entry name" value="VPS45_Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56815">
        <property type="entry name" value="Sec1/munc18-like (SM) proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003023-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="587" mass="66875" checksum="80620E2FF50C541E" modified="2020-08-12" version="1">MALKLLVGQKIMNEVVRSKTKSDGKPGTEWRILIVDKLAMRMVSACTKMHEISAEGVTLVEDINKKREPLPAIEAVYLITPSEDSIRLLMRDFENPAKPTYKAAHVFFTEVCPEELFNDICKSVVSRKIKTLKEINIAFLPYESQVYSLDSPVTFQCAYSPALASARSGNMERIAEQIATLCATLGEYPSVRYRSEWDGNVELAQMVQQKLDAYKADEPTMGEGPEKARSQLLILDRGFDCVSPLLHELTLQAMAYDLLPIVNDVYKFIPSPNAAEKEVLLDENDDLWVDLRHQHIAVVSQSVTQYLKSFTESKRLTQSEKQSMKDLSQMIKKMPQYQKQLSKYSTHLHLAEDCMKSYQGYVDKLCRVEQDLAMGTDAEGEKIKDHMRNIVPILLDQNVSNYDKVRIIALYVMIKNGISEENLTKLVTHAQIDQKEREMIHNLIHLGINVIADGNRKKSYTVPRKERINEHTYQMSRWTPVIKDIMEDAIDNKLDERHFPFLGGRKMAGFHAPTSARYGHWHKDKSQTAVKNVPRLIMFVVGGCSYSEIRCAYEVTAAVKNWEVYIGSSHILTPETFLSDLGSLNKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VFM3</accession>
    <name>A0A6E8VFM3_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HECT-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8VFM3"/>
    <dbReference type="EnsemblMetazoa" id="ACON003133-RA">
        <property type="protein sequence ID" value="ACON003133-PA"/>
        <property type="gene ID" value="ACON003133"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000842"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003133"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041870"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019871">
        <property type="term" value="F:sodium channel inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045879">
        <property type="term" value="P:negative regulation of smoothened signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048260">
        <property type="term" value="P:positive regulation of receptor-mediated endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048814">
        <property type="term" value="P:regulation of dendrite morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000017">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2160.10:FF:000001">
        <property type="entry name" value="E3 ubiquitin-protein ligase NEDD4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2410.10:FF:000001">
        <property type="entry name" value="E3 ubiquitin-protein ligase NEDD4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1750.10:FF:000001">
        <property type="entry name" value="E3 ubiquitin-protein ligase NEDD4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050409">
        <property type="entry name" value="E3_ubiq-protein_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254:SF440">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE NEDD-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254">
        <property type="entry name" value="HECT DOMAIN UBIQUITIN-PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 9" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="C2" evidence="11">
        <location>
            <begin position="2"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="12">
        <location>
            <begin position="217"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="12">
        <location>
            <begin position="530"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="12">
        <location>
            <begin position="605"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="domain" description="HECT" evidence="13">
        <location>
            <begin position="697"/>
            <end position="1027"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="270"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="329"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="569"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="365"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="402"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="485"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="511"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="579"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="9">
        <location>
            <position position="995"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003133-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1028" mass="112087" checksum="D15C8C8C3386DCA2" modified="2020-08-12" version="1">MTRTGMLINLNEEDVCYVRIRVLGASGLAKKDIFGYSDPYVKIEQNTITGDVNVDHMVTKTKRRTLNPVWNEEFVFRVKPNEHKLVFQVFDENRLTRDGFMGLVDLTLINLPHESDQRTIAPQRYTLRAKRQSSLRVSTKVRGTLELYHAIMRDDDPAIGRTRSAIGGSNRASATSTPLAAHRNAGLYPQQQLPLNHPQHPQHRLHQLASLTAQPNDALPPGWEVRQDPVGRMYYVNHIARTTQWERPTIGPSQPGLNEMVAAFQRRFHISNDPDNGGSGSNNTSMADTVSITSENGDTTAAAVGNRSGAPGIGSPMVLAADHPLLSTLMAQSGDGGGRGTAVATPERPASAPRGDDAELTVGLSPSPSVASIASSGAGGTSIRPIRPAPPPPPGGGGGGSSASSARASPVGTPVPSQRRRSSPGSSESAQATAIEASSGEAGDTTPGPSAPIATADDEQPNASAPVNGSAEGGAPSTVPREVSETATSSIGSEQTAATTPNRPDNAVAVQPTSPSHPTTPAPAAQPNATGLPPGWAVQVASNGRLFFIDHINKTTSWVDPRTGLASPIPSAAGSDGVAAGGAGSTAGSGHGSRGDSRSSDDNLGPLPEGWEERVHSDGRTFFIDHNTRTTQWDDPRLSNPKIAGQAVPYSRDYKQKYEYFKSQLQKPSNVPNKIDIKVRRASILEDSYRVINSVTRLDLLKTKLWVEFEGETGLDYGGLAREWFYLLSKEMFNPYYGLFEYSAMDNYTLQINPNSGLCNEEHLFIGRIAGMAIYHGKLLDAFFIRPFYKMMLQKSIDLKDMEAVDTEYYNSLLYIKENDPSTLMLTFSVDEESFGTTNQRELKPNGADLEVSNENKDEYIRLVIDWRFEARVKDQMQAFLEGVSSLVPLHLLKIFDENELELLMCGIQSIDVNDWKKNTMYKGDYYANHAVVQWFWRAVLSFNNEMRSRLLQFVTGTSRVPMNGFKELYGSNGPQLFTIEKWGTVNNFPRAHTCFNRLDLPPYESYAQLKEKLISAIEGSQGFAGVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VHL2</accession>
    <name>A0A6E8VHL2_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="9">C-terminal binding protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040228217.1">
        <property type="nucleotide sequence ID" value="XM_040372283.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VHL2"/>
    <dbReference type="EnsemblMetazoa" id="ACON003893-RA">
        <property type="protein sequence ID" value="ACON003893-PA"/>
        <property type="gene ID" value="ACON003893"/>
    </dbReference>
    <dbReference type="GeneID" id="120952783"/>
    <dbReference type="CTD" id="41602"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004234"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003893"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035290"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140297">
        <property type="term" value="F:DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001221">
        <property type="term" value="F:transcription coregulator binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05299">
        <property type="entry name" value="CtBP_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000012">
        <property type="entry name" value="C-terminal-binding protein 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043322">
        <property type="entry name" value="CtBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051638">
        <property type="entry name" value="CTBP_dehydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029753">
        <property type="entry name" value="D-isomer_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46029">
        <property type="entry name" value="C-TERMINAL-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46029:SF7">
        <property type="entry name" value="C-TERMINAL-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00671">
        <property type="entry name" value="D_2_HYDROXYACID_DH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase catalytic" evidence="7">
        <location>
            <begin position="34"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding" evidence="8">
        <location>
            <begin position="130"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="376"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="386"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="398"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003893-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="423" mass="45286" checksum="A10FEEF1C073ADF5" modified="2023-06-28" version="2">MMPKRSRLGDVRGPISNGPMQSRPLVALLDGRDCSIEMPILKDVATVAFCDAQSTSEIHEKVLNEAVGALMWHTIILTKEDLEKFKALRIIVRIGSGVDNIDVKAAGELGIAVCNVPGYGVEEVADTTMCLILNLYRRTYWLANMVREGKKFTGPEQVRDAAQGCARIRGDTLGLVGLGRIGSAVALRAKVFGFNVSFYDPYLPDGIEKSLGLNRVYTLQELLFHSDCVSLHCTLNEHNHHLINEFTIKQMRPGAFLVNTARGGLVDDEALAVALKQGRIRAAALDVHENEPYNGALKDAPNLLCTPHAAFYSEAATTELREMAASEIRRAIIGNIPECLRNCVNKEYFLRSSTGGGAGGYSEAGINGGYYSGGLQQAHSTTPLEAPHSAGSHAPPSGGGPPPPPVAVIPPVSAVTAPPPQAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VHM1</accession>
    <name>A0A6E8VHM1_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Rieske domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">Ngousso</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAD-dependent oxidoreductase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040230413.2">
        <property type="nucleotide sequence ID" value="XM_040374479.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VHM1"/>
    <dbReference type="EnsemblMetazoa" id="ACON003904-RB">
        <property type="protein sequence ID" value="ACON003904-PB"/>
        <property type="gene ID" value="ACON003904"/>
    </dbReference>
    <dbReference type="GeneID" id="120953992"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006472"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003904"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031094"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03478">
        <property type="entry name" value="Rieske_AIFL_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.102.10.10:FF:000003">
        <property type="entry name" value="apoptosis-inducing factor 3 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.10.10">
        <property type="entry name" value="Rieske [2Fe-2S] iron-sulphur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050446">
        <property type="entry name" value="FAD-oxidoreductase/Apoptosis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036922">
        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43557">
        <property type="entry name" value="APOPTOSIS-INDUCING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43557:SF2">
        <property type="entry name" value="RIESKE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00355">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="FAD/NAD-linked reductases, dimerisation (C-terminal) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50022">
        <property type="entry name" value="ISP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="6" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rieske" evidence="10">
        <location>
            <begin position="31"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003904-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="553" mass="59631" checksum="8C0AFBA470C29F63" modified="2020-08-12" version="1">MMSYLASKVTAATSTSNGGAAGTHDDDFIEAVVCQESEIGENQMKQVELGEGKVLLVRQNGKLSAIGNKCSHYGAMLVTGALGEGRVRCPWHGACFNVETGDIEDFPGMDSLPCYRVTVGEAGEVKVRAKRTELATNKRTRVMAKRAAQDARTYVVIGGGPSGATCAETLRQEGFTGRIVMINKEPCLPYDRVKVSKTMDLNLEKVLLRTQQFYDENDIEVMLGTAVTKLDGATHELTLDNGYKIRYDKAYIATGSNPRRPPIEGASLGNVCVLRTAADAKQVNEQLAPEKRVVILGTSFIGLEAAAYCVNKVAKVTVIGRGAVPLKESFGDAIGQRVMELFEEKGVQFVMNSGIRRCIGAEGTVQQVELTDGTLFDADICIFGIGSTLYTEFLQGSGISLNRNGSINTDQYLESNLEGVYVGGDIANAPVHSNDGQQATIGHYPLAQYHGRVAALNMIGKATPLKAVPFFWTVLFGKSFRYCGYGQPDEVIIDGDLAALKFVAFYIGKGGRVIGMSSCQRDPVVAQFAEYSSQGKVLHKEDLTPNPLGWVPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VHN5</accession>
    <name>A0A6E8VHN5_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="8">PUM-HD domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VHN5"/>
    <dbReference type="EnsemblMetazoa" id="ACON003914-RA">
        <property type="protein sequence ID" value="ACON003914-PA"/>
        <property type="gene ID" value="ACON003914"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON003914-RB">
        <property type="protein sequence ID" value="ACON003914-PB"/>
        <property type="gene ID" value="ACON003914"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000912"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003914"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039292"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010608">
        <property type="term" value="P:post-transcriptional regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07920">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000004">
        <property type="entry name" value="Pumilio homolog 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033133">
        <property type="entry name" value="PUM-HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033712">
        <property type="entry name" value="Pumilio_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001313">
        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537:SF12">
        <property type="entry name" value="MATERNAL PROTEIN PUMILIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537">
        <property type="entry name" value="RNA BINDING PROTEIN PUMILIO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00806">
        <property type="entry name" value="PUF"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00025">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50302">
        <property type="entry name" value="PUM"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50303">
        <property type="entry name" value="PUM_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="PUM-HD" evidence="7">
        <location>
            <begin position="404"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="5">
        <location>
            <begin position="424"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="5">
        <location>
            <begin position="460"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="5">
        <location>
            <begin position="496"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="5">
        <location>
            <begin position="532"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="5">
        <location>
            <begin position="568"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="5">
        <location>
            <begin position="604"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="5">
        <location>
            <begin position="640"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="5">
        <location>
            <begin position="679"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="204"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="383"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="768"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="212"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="768"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="816"/>
            <end position="855"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003914-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="869" mass="91020" checksum="75D01BD02DCAC939" modified="2020-08-12" version="1">MVVLEGGTMGTSLLGGPYAQGAPALKMMQKRYVGLHQWLSPMCATKDLKEHLQLFRSNPGLTAANSAVPGAPNGQPLPQSALSLSAAQQQQFLQHNAAYAAQQAPPYVINPGQDPNQYMGALIAAGVPQYYAPAPWGVYPANLAIPQQQSQPRRPLTPSQQGAENQPYQVISAYYDQSGSLVMGPRTGTPMRLVSPAPPVLVPPGAAGPRAPQAPPMYPPQPQTAQQNVYSAQNGSNVGGLGALNTSSLTTRRDSFDRNTSAFSPSLDYTSGASTNTMAMNAAAAAATAAARKWPVGAGNYSGLGTVAAASASPLGPSLTPPPVSGLGTLVTSRAPGAETKYRQVNPSLVPGISTGNAAMFGSNNCAAQAILSRLSSAVARPTTAPAPAPAVPGAAPGLDRPPGRSRLLEDFRNQRYPNLQLRDLTNHIVEFSQDQHGSRFIQQKLERAISVEKQLVFNEILGAAYSLMTDVFGNYVIQKFFEFGSPEQKQALAQQVKGHVLPLALQMYGCRVIQKALESIPAEQQQEIVRELDGHVLKCVKDQNGNHVVQKCIECVDPVALQFIIDAFRNQVYSLSTHPYGCRVIQRILEHCTPEQTAPILAELHANTEHLIQDQYGNYVIQHVLEHGKPEDKSALIASVRGKVLILSQHKFASNVVEKCVTHATRAERALLIEEVCSFNDSGLHVMMKDQYANYVVQKMIDVSEPTQRKVLLHKIRPHMNSLKKYTYGKHIIAKLDKFTLKTPNSLAGNGAGGSGNAAAASATGTAANGTAGSAAGGPATNGTNGSANSTTGELTANGPSNGAGVSNGGGNANGAGATATGGSSASNGAGVPSNGVTTNSNTNVSGGNSNGTSAGLRPIGPPTNGVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VHQ3</accession>
    <name>A0A6E8VHQ3_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="6">PDZ domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VHQ3"/>
    <dbReference type="EnsemblMetazoa" id="ACON003937-RD">
        <property type="protein sequence ID" value="ACON003937-PD"/>
        <property type="gene ID" value="ACON003937"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007039"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003937"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031607"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031941">
        <property type="term" value="C:filamentous actin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001725">
        <property type="term" value="C:stress fiber"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051371">
        <property type="term" value="F:muscle alpha-actinin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061061">
        <property type="term" value="P:muscle structure development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000055">
        <property type="entry name" value="PDZ and LIM domain protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050604">
        <property type="entry name" value="PDZ-LIM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24214">
        <property type="entry name" value="PDZ AND LIM DOMAIN PROTEIN ZASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24214:SF38">
        <property type="entry name" value="PDZ AND LIM DOMAIN PROTEIN ZASP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0440">LIM domain</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PDZ" evidence="5">
        <location>
            <begin position="13"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="90"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="181"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="90"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="131"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="181"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="221"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="263"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003937-PD"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="276" mass="30305" checksum="41C74B0A794642D3" modified="2020-08-12" version="1">MLTVSSRDGPAASENGDNPLGFRITGGADFEMPITVFQVSEGSPAQKAGLQLGDQILKINGADASAMRLATAQSVIKQAGEQLQMIVAKDDENNRKAEEQGQPKETREVKFAGNEQEPAADVTNKPAPQPKNDERENWTQPPERKVWHPIVWQQPPPPIPPDLYGKDAPHQQLIANIRRLLTETRTKPEERQKHIERMMLSLPTGSRREGEEDEEEPKPAPKPKKKKKSKAKKAKAAPRTRLDSETSEDGSVSELTGPAAAEPAEEPIPEEASVEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VHQ4</accession>
    <name>A0A6E8VHQ4_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="6">PDZ domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VHQ4"/>
    <dbReference type="EnsemblMetazoa" id="ACON003939-RA">
        <property type="protein sequence ID" value="ACON003939-PA"/>
        <property type="gene ID" value="ACON003939"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010454"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003939"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032779"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:stress fiber"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051371">
        <property type="term" value="F:muscle alpha-actinin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PDZ and LIM domain protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PDZ"/>
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        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24214">
        <property type="entry name" value="PDZ AND LIM DOMAIN PROTEIN ZASP"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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            <begin position="4"/>
            <end position="87"/>
        </location>
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            <begin position="138"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
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            <begin position="225"/>
            <end position="236"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="252"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="411"/>
            <end position="423"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="425"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003939-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="595" mass="66341" checksum="BDCBF677C084E606" modified="2020-08-12" version="1">MVLDLNFSRYDNTTPFGFKLVGGADFDVPLTVVKILPGSIAEDVGMHMGDVVVRINDIPTGNMSYYDAHQLLACAGNQFVLGILRAREVTPSQRLIKEKTPSGEVEYINTSPKPFWTPNVGSPSPLAVHHPAKEEPRQQQQQPDEQIPEVPITDEQIAEILSGEAEVLKQHNVIGVNFNKMIPKAGVFKQSEVFKTLNSELVQSEEEDKKWTTFLQKPSRPPPKTPEERKLERNPPTERYQVRIVKQPKPKIAPDYKPPKSPEPEPEPEPIPMYDEYLNERQEVEVETIKTLVEEPVVPSEPMAGEGEAQQPGAPNPTEEEPSEEFREQLQNVQKQLEALSALPNTIQATIAALTEQLAVLAAPKRKSKSISPRPEEADPSAGGEGTEQSAPEAIVPEVSETFEVTEFSRADSSGSGDQSSSVDEGEHIHYTEEELEQLEKRMKEHDIEWTNQNDKDSDSSSQHNAGGDIRTAVQDELKLQVVKKKKKAVSAFGPLTPQERPIYLPGGRKWRTAKDAFNEQFIAEVISSQAELIQGTTLGVNFLKYQKPEKDLSFIKNSDVYKLVHDMDPPKSGIELRPEMVVAEEDVRKCASPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VHQ8</accession>
    <name>A0A6E8VHQ8_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="6">PDZ domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VHQ8"/>
    <dbReference type="EnsemblMetazoa" id="ACON003939-RD">
        <property type="protein sequence ID" value="ACON003939-PD"/>
        <property type="gene ID" value="ACON003939"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010454"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003939"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032779"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PDZ-LIM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24214">
        <property type="entry name" value="PDZ AND LIM DOMAIN PROTEIN ZASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24214:SF38">
        <property type="entry name" value="PDZ AND LIM DOMAIN PROTEIN ZASP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0440">LIM domain</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PDZ" evidence="5">
        <location>
            <begin position="4"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="113"/>
            <end position="149"/>
        </location>
    </feature>
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        <location>
            <begin position="205"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="363"/>
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        </location>
    </feature>
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        <location>
            <begin position="408"/>
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        <location>
            <begin position="138"/>
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        </location>
    </feature>
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        <location>
            <begin position="225"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="252"/>
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        </location>
    </feature>
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        <location>
            <begin position="411"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003939-PD"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="511" mass="57162" checksum="75008029B986533B" modified="2020-08-12" version="1">MVLDLNFSRYDNTTPFGFKLVGGADFDVPLTVVKILPGSIAEDVGMHMGDVVVRINDIPTGNMSYYDAHQLLACAGNQFVLGILRAREVTPSQRLIKEKTPSGEVEYINTSPKPFWTPNVGSPSPLAVHHPAKEEPRQQQQQPDEQIPEVPITDEQIAEILSGEAEVLKQHNVIGVNFNKMIPKAGVFKQSEVFKTLNSELVQSEEEDKKWTTFLQKPSRPPPKTPEERKLERNPPTERYQVRIVKQPKPKIAPDYKPPKSPEPEPEPEPIPMYDEYLNERQEVEVETIKTLVEEPVVPSEPMAGEGEAQQPGAPNPTEEEPSEEFREQLQNVQKQLEALSALPNTIQATIAALTEQLAVLAAPKRKSKSISPRPEEADPSAGGEGTEQSAPEAIVPEVSETFEVTEFSRADSSGSGDQSSSVDEGEHIHYTEEELEQLEKRMKEHDIEWTNQNDKPKYQTIEWAIVSQRYRIYVDEEEEVEYVTIPLRISQDLEACGVAATAIEPQPVLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VI06</accession>
    <name>A0A6E8VI06_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA topoisomerase I</fullName>
            <ecNumber evidence="7">5.6.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">DNA topoisomerase 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at the specific target site 5'-[CT]CCTTp site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6 7">
            <text>ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.</text>
            <dbReference type="EC" id="5.6.2.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 6 7">Belongs to the type IB topoisomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.1" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8VI06"/>
    <dbReference type="EnsemblMetazoa" id="ACON004078-RB">
        <property type="protein sequence ID" value="ACON004078-PB"/>
        <property type="gene ID" value="ACON004078"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009159"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004078"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038986"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003917">
        <property type="term" value="F:DNA topoisomerase type I (single strand cut, ATP-independent) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00659">
        <property type="entry name" value="Topo_IB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03488">
        <property type="entry name" value="Topoisomer_IB_N_htopoI_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.41:FF:000001">
        <property type="entry name" value="DNA topoisomerase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.132.10:FF:000001">
        <property type="entry name" value="DNA topoisomerase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.11.10:FF:000002">
        <property type="entry name" value="DNA topoisomerase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.15.10:FF:000001">
        <property type="entry name" value="DNA topoisomerase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.11.10">
        <property type="entry name" value="DNA Topoisomerase I, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.15.10">
        <property type="entry name" value="Topoisomerase I, Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.41">
        <property type="entry name" value="Yeast DNA topoisomerase - domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013034">
        <property type="entry name" value="DNA_topo_DNA_db_N_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013030">
        <property type="entry name" value="DNA_topo_DNA_db_N_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001631">
        <property type="entry name" value="TopoI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025834">
        <property type="entry name" value="TopoI_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014711">
        <property type="entry name" value="TopoI_cat_a-hlx-sub_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014727">
        <property type="entry name" value="TopoI_cat_a/b-sub_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013500">
        <property type="entry name" value="TopoI_cat_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008336">
        <property type="entry name" value="TopoI_DNA-bd_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036202">
        <property type="entry name" value="TopoI_DNA-bd_euk_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013499">
        <property type="entry name" value="TopoI_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018521">
        <property type="entry name" value="TopoIB_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048045">
        <property type="entry name" value="Topoisomer_I_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051062">
        <property type="entry name" value="Topoisomerase_IB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10290:SF3">
        <property type="entry name" value="DNA TOPOISOMERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10290">
        <property type="entry name" value="DNA TOPOISOMERASE I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14370">
        <property type="entry name" value="Topo_C_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01028">
        <property type="entry name" value="Topoisom_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02919">
        <property type="entry name" value="Topoisom_I_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00416">
        <property type="entry name" value="EUTPISMRASEI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00435">
        <property type="entry name" value="TOPEUc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46596">
        <property type="entry name" value="Eukaryotic DNA topoisomerase I, dispensable insert domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56741">
        <property type="entry name" value="Eukaryotic DNA topoisomerase I, N-terminal DNA-binding fragment"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00176">
        <property type="entry name" value="TOPO_IB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52038">
        <property type="entry name" value="TOPO_IB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 6 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6 7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 7" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="DNA topoisomerase I eukaryotic-type" evidence="9">
        <location>
            <begin position="181"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="131"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="464"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="active site" description="O-(3'-phospho-DNA)-tyrosine intermediate" evidence="6">
        <location>
            <position position="538"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004078-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="580" mass="68216" checksum="C3C00093F52B1F5A" modified="2020-08-12" version="1">MVIPGFFSTTVQTLHRVPKRLHLTNRWEEEKRDDGVKWKYLEHKGPVFAPPYDPLPSHVKFMYDGKEMKLSQDAEEVAGFYARMLEHDYTSKQAFNDNFFKDWRKTMTPHERERITDLEKCNFRPMAAYFAEIAEQNRNRSKEEKAALKAANEQLTKEYGICVIDGHKEKIGNFKIEPPGLFRGRGEHPKMGLVKRRVMPEDVIINCSQDSKIPSPPPGRRWKEVRHDNTVSWLASWVENVQGQVKYIMLNPSSKLKGEKDWQKYETARRLLKHIDKIRDTYRDEWKSKEMRIRQRAVAMYFIDKLALRAGNEKDDDQADTVGCCSLRYEHIALHKELNGKENVVVFDFLGKDSIRYYNEVEVEKRVFKNLELFKENKKPGDDLFDRLNTSVVNEYLKGLMDGLTAKVFRTFNASYTLQKQLEELTDPNMSVPEKLLAYNRANRAVAILCNHQRAVPKTHEKSMGNLKEKIRVKREQVEQCQKEFKDLKKSNVRDPSAFDKKSKQLERLKEQLKKLELQETDRDENKTIALGTSKLNYLDPRISVAWCKKFGVPIEKIFNKTQRDKFRWAIDMADENYVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VI19</accession>
    <name>A0A6E8VI19_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Fimbrin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VI19"/>
    <dbReference type="EnsemblMetazoa" id="ACON004071-RA">
        <property type="protein sequence ID" value="ACON004071-PA"/>
        <property type="gene ID" value="ACON004071"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002366"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004071"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038170"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005884">
        <property type="term" value="C:actin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032432">
        <property type="term" value="C:actin filament bundle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051017">
        <property type="term" value="P:actin filament bundle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051639">
        <property type="term" value="P:actin filament network formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21292">
        <property type="entry name" value="CH_PLS_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21295">
        <property type="entry name" value="CH_PLS_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21298">
        <property type="entry name" value="CH_PLS_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21301">
        <property type="entry name" value="CH_PLS_rpt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000042">
        <property type="entry name" value="Fimbrin, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000071">
        <property type="entry name" value="plastin-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000263">
        <property type="entry name" value="plastin-1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000066">
        <property type="entry name" value="plastin-1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000010">
        <property type="entry name" value="Plastin-3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001589">
        <property type="entry name" value="Actinin_actin-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039959">
        <property type="entry name" value="Fimbrin/Plastin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19961:SF18">
        <property type="entry name" value="FI19014P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19961">
        <property type="entry name" value="FIMBRIN/PLASTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00019">
        <property type="entry name" value="ACTININ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00020">
        <property type="entry name" value="ACTININ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CH"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="22"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="5">
        <location>
            <begin position="126"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="5">
        <location>
            <begin position="270"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="5">
        <location>
            <begin position="392"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="5">
        <location>
            <begin position="513"/>
            <end position="621"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004071-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="656" mass="73762" checksum="19B7EF91F1F7CD5F" modified="2020-08-12" version="1">MDIHQIAKEDELLEFRRIFTPELYDQIDTNKDGFIELKELKDALDQVGFKLPGYQVRLMIDEYSNKQRSQHVGKLSYDEFESLCMDLKAKDVASTFKKVVSKKENLETLGGMSDSSAAGTTHSVRVEEQLAFSDWINSNLVHDPDLKHLLPLDSEGKLLYDKMKDGILLCKIVNHSCPDTIDERAINKKNLTVYTKFENLTLALVSSQAIGCNIVNIDAHDLAKGKPHLVLGLLWQIIRIGLFSHITLDSCPGLATLLSDGERLEDLMKLSPEAILLRWVNHHLERAGIARRCTNFQSDISDSEVYSYLLNQIAPKDAGVNVEALREQNALNRAEMMLQQAAKLNCRSFVTPQDVVNGVYKLNLAFVANLFNNHPGLDQPEEIEGLESIEETREEKTYRNWMNSMGVKPHVNWLYSDLADGLIIFQLFDIIQPGSVQWKRVHQKFTPLRKFMEKLENCNYAVELGKQQKFSLVGIAGQDLSDGNATLTLALIWQLMRAYTLSILSRLANTGNPIIEKEIVQWVNSKLQSAGKRTSLKSFQDPAIADGKIIIDLIDTIKPGSINYDNVRDGGNPEENLENAKYAVSMARKIGARVYALPEDITEVKAKMIMTVFACLMAMDYVPNMDSAKSAPPVAAVVQAPVPSSFHSHPWTTNRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VJP1</accession>
    <name>A0A6E8VJP1_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Metalloendopeptidase</fullName>
            <ecNumber evidence="11">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">Ngousso</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="10 11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="10 11">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="11"/>
    <dbReference type="RefSeq" id="XP_040229023.1">
        <property type="nucleotide sequence ID" value="XM_040373089.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VJP1"/>
    <dbReference type="EnsemblMetazoa" id="ACON004620-RA">
        <property type="protein sequence ID" value="ACON004620-PA"/>
        <property type="gene ID" value="ACON004620"/>
    </dbReference>
    <dbReference type="GeneID" id="120953286"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_008540"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004620"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031589"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04280">
        <property type="entry name" value="ZnMc_astacin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000015">
        <property type="entry name" value="Meprin A subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034035">
        <property type="entry name" value="Astacin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001506">
        <property type="entry name" value="Peptidase_M12A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10127">
        <property type="entry name" value="DISCOIDIN, CUB, EGF, LAMININ , AND ZINC METALLOPROTEASE DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10127:SF780">
        <property type="entry name" value="METALLOENDOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01400">
        <property type="entry name" value="Astacin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00480">
        <property type="entry name" value="ASTACIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51864">
        <property type="entry name" value="ASTACIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 10 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 10 11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1 10 11" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0732">Signal</keyword>
    <keyword evidence="5 10 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039962374" description="Metalloendopeptidase" evidence="11">
        <location>
            <begin position="23"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M12A" evidence="13">
        <location>
            <begin position="94"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="293"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="293"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="188"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004620-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="359" mass="38572" checksum="678ECF6722329D30" modified="2023-06-28" version="2">MSVSQILRVLAIGCFVIGCTQANVIMYDTEENSIDGSSDFIDLTHLGPNLFGEPDEEVGKLVANFNPETDAGNVEELGSYVEGDMLIDRPEGRNGLSNTATRWPNGVVPFVISGNFDAKGMQLIEQAINEYHTKTCIRFVPRMGEKNYVSFESSSSGCWSSVGMIGGKQAVNLQIPGCTTLVGTAIHEMMHALGFLHEQNREDRDDWVRIRYENIKAGTSNNFEKAKKGTTNSFGVAYDYASIMHYSSNAFSTNGNPTIEAKRAGGNKMGQRQGFSSSDLAKLNAMYGCKGNSAAATSSGTSPQTQRPGRPTRPSRPAGNNRPGNGGAQVASAIVNLIGSIFGEEDTNTTAIAGEVVRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VJR4</accession>
    <name>A0A6E8VJR4_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">PSI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VJR4"/>
    <dbReference type="EnsemblMetazoa" id="ACON004689-RB">
        <property type="protein sequence ID" value="ACON004689-PB"/>
        <property type="gene ID" value="ACON004689"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_003150"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004689"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038039"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002165">
        <property type="entry name" value="Plexin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031152">
        <property type="entry name" value="PLXDC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13055:SF12">
        <property type="entry name" value="LD40707P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13055">
        <property type="entry name" value="TUMOR ENDOTHELIAL MARKER 7 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01437">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026258619" description="PSI domain-containing protein" evidence="9">
        <location>
            <begin position="27"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="507"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="47"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004689-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="570" mass="64667" checksum="DAA149B7B92835B5" modified="2020-08-12" version="1">MMRMMLIIRGSISWLICLSLLQLCVCVKVPRKYLQSDPAEIGISSVVPLASGNGNGNTNESGTGDMPFNRSILPLDENKKRQKKVFPNDEESANKSVFPSPTKLGAVGLNTSLIADSSTRDPISDAINIDTIERTETELNSTLQEHNITKTFEDNHLYYKSTWSTDKTMSEEFWKKISTNLTVNMLLSNSHRRATTMILSFDFPFYGFPIRNVTIASGGFLYTGDYVHSWLASTQYIAPLMANFDTALSNNSFVKYRDDGETFTVVWENVILQDRPSNGTFTFSVTLNKSGDIVFAYKKIPISIQHIFENPLMKNHPVKIGLSDAYIIDKTIMRTKQKTIYEYHRVNFGQTEVTNDTIITLTSLPTCFSFKDCESCITHEGADFECLWCPTMNRCSTGTDRKRQDWVHKGCEATTISEILYCPALGQKGNNYGESMDVTSKITDRYNSTSSIRDNYQPAQDDKLHQNTNGVAQNGSYIHNGSKKEEFVNKAHLYHTTDMDSGSSNKLIVSLLVIILIMLICGCWVLYAYRNPHTKSGQLLIRIFKWKKYRPNKWLWRRGEARYTAASIHM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VJR9</accession>
    <name>A0A6E8VJR9_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ras-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="EnsemblMetazoa" id="ACON004692-RB">
        <property type="protein sequence ID" value="ACON004692-PB"/>
        <property type="gene ID" value="ACON004692"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000481"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007480"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004692"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038199"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04013">
        <property type="entry name" value="C2_SynGAP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05136">
        <property type="entry name" value="RasGAP_DAB2IP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.506.10">
        <property type="entry name" value="GTPase Activation - p120gap, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021887">
        <property type="entry name" value="DAB2P_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039360">
        <property type="entry name" value="Ras_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023152">
        <property type="entry name" value="RasGAP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001936">
        <property type="entry name" value="RasGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057606">
        <property type="entry name" value="SynGAP1-like_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10194:SF60">
        <property type="entry name" value="RAS GTPASE-ACTIVATING PROTEIN RASKOL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10194">
        <property type="entry name" value="RAS GTPASE-ACTIVATING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12004">
        <property type="entry name" value="DAB2P_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25321">
        <property type="entry name" value="PH_RASGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00616">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00323">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00509">
        <property type="entry name" value="RAS_GTPASE_ACTIV_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50018">
        <property type="entry name" value="RAS_GTPASE_ACTIV_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="4">
        <location>
            <begin position="285"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GAP" evidence="5">
        <location>
            <begin position="468"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1135"/>
            <end position="1159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1298"/>
            <end position="1325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1420"/>
            <end position="1476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1579"/>
            <end position="1601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1135"/>
            <end position="1146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1147"/>
            <end position="1158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1436"/>
            <end position="1457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1581"/>
            <end position="1592"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004692-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1601" mass="179530" checksum="BC2CEB9239EA4F59" modified="2020-08-12" version="1">MSDNSILTAKDKSKTLPQNLFLVNSMPPKSSFLHHSSSRIGNNSTFDSKKSSSIAGLSSKLPAPAMDYMISPTKSISFIRRTHSTKLSRSNSLLKSLTSKCVDQSGCNTSLLRIPVKELEYDRLCRILEKPAQLDHMIRDIFILCKDEKVEENAVHSDTSYEKACRRGSAPSTPVMGQKGESTSRFTNFFSKRSFRSIPLKRTKSVIKLERSKRGQGGIRGSRSHESLLSTHVVMSNIDLACIGTIEVVPVHSSVLGRRHCFQVRGISHEERYYSCGTRQERDIWIHSLRKSISPNKDNRRRLDNSLKMWVYEAKSLPPKKRYFCEIYIDKTLYGRTSVKLRADLLFWGEYFDFPDIPEASIITVNVYREAEKKKKKDKHVLVGSVVIPIEKVAARTFSEDWYQILMEKQDNVIKSPSKEPAPTLRIKCRYQSIDIMPLDVYGEFHEFLKKNYKKICEVIEPIIGVKAKEDIGQALVLQMHSQGMAAIFLSDVVALDLLRVDDQRLTFRGNSLATKSMEAFLKLIGEQYLQDTLSIPIAEIIASDRDCEVDPTKVNGSLSRQQQALRRTVKSVWIAIAESSKNFPKQLRDCFATFRERLHDLDREDMADNLISASIFLRFLCPAIMSPSLFNITNELPSARATRNLTLVAKTLQTLANFTRFQGKENFMEFLNDFLEQEAPRMKQFLYDISCSNFSNPDNFMDWSGCIDQGKQLSILHSLLYEIVLKLPADKQHEIQPLPAILATITHCKQVNCTTLNADQYCTGKPHIVQVKENLLSKKEDILQSAGSANMSSKEYSTKRNPKILKNECTVQSSNTLQPSTHSSSFSLHYSTSTSTNSLVNNTKQLDKDATDKNDDICAMGKLNFNSQYGSNIICSGSLRTYSSTGATSFGAVNSLMTNSLRNEKEKSNNLQGDIRANTLPRYNNYTTSVSIRENTYPESSNSVGYDHEVNGNSRLNGINKNLIQIDIDPTNPINRKSPTPLLKNISCCHYIANNRIQEGSHQRLTSPGSNLSDLDKNAFNLGIPHNDTNREQQYSQHIYPAPALKASNITTISSFVPRQSTTTFNPSKMPMSLEDLEDLLNYADEQNQTSFSESVSNRGGCDPCGTNTKANIASNGSNASISQISNICSSGYQSIPTQSTSSSPVESTGEQHSLVTNRAPPSRKLTTTLIGYNIGTSQKLCMNDQAIAKADLPVSSINMQLSNKSDPLVTSHGTIHHYYDRKTPNEQSNVNRILNNNYVDISYDVLSPCKSPIASKKTECTSLGIDEHNDLAHPVEYFGINRTNHHSRHKRKVIHGIRRSGSSIQSESSDDERVSNTTSENFNDIDSLTTGNVSALERNHILISNGNDYDQASSGLFGCRLGCRRMPRTNPLMQYDKDDVSDIIGNSGVDTALTNHQRNQSFQSRFHRRLSIECARALSDSSTDETESEKTKTFNSNQHTAQHHNLSISTTTHTNIFHENDTRRRRNANKSVEQCEREIQRLQASLDSMRKKLEMSEPAEGNANKLAAITQQSDSKIRSIIGRLLTMEEELRQEQYKMSLVLSHKQRVIEAQGKQIAALDAANNRLLSALSTLQNRYESQSNQSDDTSSSHPNAGASSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VJT7</accession>
    <name>A0A6E8VJT7_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Homeobox domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Antp homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040241378.2">
        <property type="nucleotide sequence ID" value="XM_040385444.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VJT7"/>
    <dbReference type="EnsemblMetazoa" id="ACON004661-RB">
        <property type="protein sequence ID" value="ACON004661-PB"/>
        <property type="gene ID" value="ACON004661"/>
    </dbReference>
    <dbReference type="GeneID" id="120961603"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005276"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004661"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032832"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009952">
        <property type="term" value="P:anterior/posterior pattern specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000193">
        <property type="entry name" value="Ultrabithorax, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050296">
        <property type="entry name" value="Antp_homeobox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001827">
        <property type="entry name" value="Homeobox_Antennapedia_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45659">
        <property type="entry name" value="HOMEOBOX PROTEIN HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45659:SF21">
        <property type="entry name" value="HOMEOTIC PROTEIN ULTRABITHORAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00032">
        <property type="entry name" value="ANTENNAPEDIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4 7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 7 8" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="11">
        <location>
            <begin position="217"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="7">
        <location>
            <begin position="219"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="87"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="271"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="87"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="111"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="156"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009107"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
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    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004661-PB"/>
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    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="310" mass="33188" checksum="7EB6B132ADC67323" modified="2020-08-12" version="1">MNSYFEQTGFYGHPHQAAAGMMTTTGTHHDQTTAAAAAAYRGFPLSLGMSPYTNHHLHQTRTAQESPYDASIQAACKQIYEGSYSSKDCGTKGTSGNNGTDTSNGYKDVWNANSGATNGATTGATGSNVPAQQNSSVPVRPSACTPDSRVGGYIDASGGSPVSRAGSAAAAAGVPGSWNTNQCSLTGSTGGQAAPSTGLHQSNHTFYPWMAIAGANGLRRRGRQTYTRYQTLELEKEFHTNHYLTRRRRIEMAHALCLTERQIKIWFQNRRMKLKKEIQAIKELNEQEKQAQAQKAAAAAAAAAALHEQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VJU3</accession>
    <name>A0A6E8VJU3_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Sodium channel protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="22">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 18">Cell membrane</location>
            <topology evidence="1 18">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the sodium channel (TC 1.A.1.10) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON004707-RM">
        <property type="protein sequence ID" value="ACON004707-PM"/>
        <property type="gene ID" value="ACON004707"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010356"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004707"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034190"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001518">
        <property type="term" value="C:voltage-gated sodium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005248">
        <property type="term" value="F:voltage-gated sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0086010">
        <property type="term" value="P:membrane depolarization during action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019228">
        <property type="term" value="P:neuronal action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13433">
        <property type="entry name" value="Na_channel_gate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000061">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000046">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000047">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="1.20.120.350:FF:000019">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="1.20.120.350:FF:000022">
        <property type="entry name" value="Sodium channel protein"/>
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        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="1.20.120.350:FF:000026">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="4"/>
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        <property type="entry name" value="EF_hand_dom"/>
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        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058542">
        <property type="entry name" value="IQ_SCN5A_C"/>
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    <dbReference type="InterPro" id="IPR001696">
        <property type="entry name" value="Na_channel_asu"/>
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        <property type="entry name" value="Na_chnl_inactivation_gate"/>
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        <property type="entry name" value="Na_trans_assoc_dom"/>
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    <dbReference type="InterPro" id="IPR024583">
        <property type="entry name" value="Na_trans_cytopl"/>
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    <dbReference type="InterPro" id="IPR043203">
        <property type="entry name" value="VGCC_Ca_Na"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
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    <dbReference type="PANTHER" id="PTHR10037:SF288">
        <property type="entry name" value="SODIUM CHANNEL PROTEIN PARA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10037">
        <property type="entry name" value="VOLTAGE-GATED CATION CHANNEL CALCIUM AND SODIUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24609">
        <property type="entry name" value="IQ_SCN5A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06512">
        <property type="entry name" value="Na_trans_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11933">
        <property type="entry name" value="Na_trans_cytopl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00170">
        <property type="entry name" value="NACHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="17 18" id="KW-0407">Ion channel</keyword>
    <keyword evidence="12 18" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0691">RNA editing</keyword>
    <keyword evidence="11 18" id="KW-0915">Sodium</keyword>
    <keyword evidence="3 18" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="16 18" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="7 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 18" id="KW-0813">Transport</keyword>
    <keyword evidence="9 18" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="127"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="198"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="232"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="256"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="387"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="777"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="821"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="850"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="905"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="985"/>
            <end position="1006"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1295"/>
            <end position="1317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1323"/>
            <end position="1349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1370"/>
            <end position="1401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1491"/>
            <end position="1517"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1580"/>
            <end position="1598"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1610"/>
            <end position="1629"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1641"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1704"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1797"/>
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        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="21">
        <location>
            <begin position="1834"/>
            <end position="1869"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
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            <begin position="27"/>
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    <feature type="region of interest" description="Disordered" evidence="20">
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            <begin position="1126"/>
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    <feature type="region of interest" description="Disordered" evidence="20">
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            <begin position="1984"/>
            <end position="2028"/>
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        <location>
            <begin position="413"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="30"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="1153"/>
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    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="20">
        <location>
            <begin position="1180"/>
            <end position="1196"/>
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    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="20">
        <location>
            <begin position="1996"/>
            <end position="2024"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
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    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022461"/>
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    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022495"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
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    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU361132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
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    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004707-PM"/>
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    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="2092" mass="234659" checksum="E6B8BCFF4EBB05DA" modified="2020-08-12" version="1">MTEDSDSISEEERSLFRPFTRESLQAIEARIADEEAKQRELERKRAEGEDEDEGPQPDPTLEQGVPVPVRMQGSFPPELASTPLEDIDSFYSNQRTFVVISKGKDIFRFSATNALYVLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILQPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKEFPLDGSWGNLTDESWELFNSNDTNWFYSESGDIPLCGNSSGAGQCDEGYICLQGYGKNPNYGYTSFDTFGWAFLSAFRLMTQDYWENLYQLVLRSAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEALREAEEAAAAKAAKLEAQQAAAAAAANPEIAKSPSDFSCHSYELFVGQEKGNDDNNKEKMSIRSEGLESVSEITRTTAPTATAAGTAKARKVSAASLSLPGSPFNLRRGSRGSHQFTIRNGRGRFVGVPGSDRKPLVLSTYLDAQEHLPYADDSNAVTPMSEENGAIIVPVYYANLGSRHSSYTSHQSRISYTSHGDLLGGMTKESRLRNRSARNTNHSIVPPPNANNLSYAETNHKGQRDFDLTQDCTDDAGKIKHNDNPFIEPAQTQTVVDMKDVMVLNDIIEQAAGRHSRASDHGVSVYYFPTEDDDEDGPTFKDKALEFLMKMIDIFCVWDCCWVWLKFQEWVAFIVFDPFVELFITLCIVVNTLFMALDHHDMDPDMEKALKSGNYFFTATFAIEATMKLIAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTVGALGNLTFVLCIIIFIFAVMGMQLFGKNYTDNVDRFPDHDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMLVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNETNKIAEAFNRISRFSNWIKMNLANALKFVKNKLTSQIASVQPTGKGVCPCISSEHGENELELTPDDILADGLLKKGIKEHNQLEVAIGDGMEFTIHGDLKNKAKKNKQIMNNSKDDDTASIKSYGSHKNRPFKDESHKGSAETMEGEEKRDASKEDLGIDEELDDEGEGDEGPLDGELIIHAEEDEVIEDSPADCCPDNCYKKFPVLAGDDDAPFWQGWGNLRLKTFQLIENKYFETAVITMILLSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFIIVMVSLINFVASLCGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCVDKNKTTLPHEIIPDVNACKAENYSWENSPMNFDHVGKAYLCLFQVATFKGWIQIMNDAIDSRDVSFVGKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTNKKFDMIIMLFIGFNMLTMTLDHYKQSETFSAVLDYLNMIFICIFSSECLMKIFALRYHYFIEPWNLFDFVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKDKSGLDDVYNFKTFGQSMILLFQMSTSAGWDGVLDGIINEEDCLPPDNDKGYPGNCGSSTIGITYLLAYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPDGTQYVRYDQLSDFLDVLEPPLQIHKPNRYKIISMDIPICRGDMMFCVDILDALTKDFFARKGNPIEETAELGEVQQRPDEVGYEPVSSTLWRQREEYCARLIQHAWKRYKQRHGGGTDASGDDLEIDACDNGCGGGNGNENDDSGDGATGSGDNGSQHGGGSISGGGGTPGGGKSKGIIGSTQANIGIVDSNISPKESPDSIGDPQGRQTAVLVESDGFVTKNGHRVVIHSRSPSITSRTADV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VK13</accession>
    <name>A0A6E8VK13_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Sodium channel protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="22">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 18">Cell membrane</location>
            <topology evidence="1 18">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the sodium channel (TC 1.A.1.10) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON004707-RB">
        <property type="protein sequence ID" value="ACON004707-PB"/>
        <property type="gene ID" value="ACON004707"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010356"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004707"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034190"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001518">
        <property type="term" value="C:voltage-gated sodium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005248">
        <property type="term" value="F:voltage-gated sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0086010">
        <property type="term" value="P:membrane depolarization during action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019228">
        <property type="term" value="P:neuronal action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13433">
        <property type="entry name" value="Na_channel_gate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000061">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000046">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000047">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000019">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000022">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000023">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000026">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058542">
        <property type="entry name" value="IQ_SCN5A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001696">
        <property type="entry name" value="Na_channel_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044564">
        <property type="entry name" value="Na_chnl_inactivation_gate"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010526">
        <property type="entry name" value="Na_trans_assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024583">
        <property type="entry name" value="Na_trans_cytopl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043203">
        <property type="entry name" value="VGCC_Ca_Na"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10037:SF288">
        <property type="entry name" value="SODIUM CHANNEL PROTEIN PARA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10037">
        <property type="entry name" value="VOLTAGE-GATED CATION CHANNEL CALCIUM AND SODIUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24609">
        <property type="entry name" value="IQ_SCN5A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06512">
        <property type="entry name" value="Na_trans_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11933">
        <property type="entry name" value="Na_trans_cytopl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00170">
        <property type="entry name" value="NACHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="17 18" id="KW-0407">Ion channel</keyword>
    <keyword evidence="12 18" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0691">RNA editing</keyword>
    <keyword evidence="11 18" id="KW-0915">Sodium</keyword>
    <keyword evidence="3 18" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="16 18" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="7 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 18" id="KW-0813">Transport</keyword>
    <keyword evidence="9 18" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="144"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="215"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="249"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="273"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="404"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="773"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="817"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="846"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="901"/>
            <end position="923"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="943"/>
            <end position="960"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="981"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1278"/>
            <end position="1296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1317"/>
            <end position="1339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1345"/>
            <end position="1371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1392"/>
            <end position="1423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1510"/>
            <end position="1536"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1599"/>
            <end position="1617"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1629"/>
            <end position="1648"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1660"/>
            <end position="1679"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1723"/>
            <end position="1746"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1816"/>
            <end position="1836"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="21">
        <location>
            <begin position="1853"/>
            <end position="1888"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="35"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1156"/>
            <end position="1218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="2003"/>
            <end position="2078"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="19">
        <location>
            <begin position="430"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="35"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="20">
        <location>
            <begin position="63"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="1175"/>
            <end position="1201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="20">
        <location>
            <begin position="1202"/>
            <end position="1218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="20">
        <location>
            <begin position="2015"/>
            <end position="2043"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU361132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004707-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="2111" mass="237181" checksum="3248A54F003B1F8E" modified="2020-08-12" version="1">MTEDSDSISEEERSLFRPFTRESLQAIEARIADEEAKQRELERKRAEGESDFGRKKKKKEIRYDDEDEDEGPQPDPTLEQGVPVPVRMQGSFPPELASTPLEDIDSFYSNQRTFVVISKGKDIFRFSATNALYVLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILQPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKEFPLDGSWGNLTDESWELFNSNDTNWFYSESGDIPLCGNSSGAGQCDEGYICLQGYGKNPNYGYTSFDTFGWAFLSAFRLMTQDYWENLYQLVLRSAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEALREAEEAAAAKAAKLEAQQAAAAAAANPEIAKSPSDFSCHSYELFVGQEKGNDDNNKEKMSIRSEGLESVSEITRTTAPTATAAGTAKARKVSAFTIRNGRGRFVGVPGSDRKPLVLSTYLDAQEHLPYADDSNAVTPMSEENGAIIVPVYYANLGSRHSSYTSHQSRISYTSHGDLLGGMTKESRLRNRSARNTNHSIVPPPNANNLSYAETNHKGQRDFDLTQDCTDDAGKIKHNDNPFIEPAQTQTVVDMKDVMVLNDIIEQAAGRHSRASDHGVSVYYFPTEDDDEDGPTFKDKALEFLMKMIDIFCVWDCCWVWLKFQEWVAFIVFDPFVELFITLCIVVNTLFMALDHHDMDPDMEKALKSGNYFFTATFAIEATMKLIAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYVDNVDRFPDHDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMLVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNETNKIAEAFNRISRFSNWIKMNLANALKFVKNKLTSQIASVQPTGKGVCPCISSEHGENELELTPDDILADGLLKKGIKEHNQLEVAIGDGMEFTIHGDLKNKAKKNKQIMNNSKVIGNSISNHQDNKLDHELNHRGVSLQDDDTASIKSYGSHKNRPFKDESHKGSAETMEGEEKRDASKEDLGIDEELDDEGEGDEGPLDGELIIHAEEDEVIEDSPADCCPDNCYKKFPVLAGDDDAPFWQGWGNLRLKTFQLIENKYFETAVITMILLSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFIIVMVSLINFVASLCGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCVDKNKTTLPHEIIPDVNACKAENYSWENSPMNFDHVGKAYLCLFQVATFKGWIQIMNDAIDSRDVGKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTNKKFDMIIMLFIGFNMLTMTLDHYKQSETFSAVLDYLNMIFICIFSSECLMKIFALRYHYFIEPWNLFDFVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKDKSGLDDVYNFKTFGQSMILLFQMSTSAGWDGVLDGIINEEDCLPPDNDKGYPGNCGSSTIGITYLLAYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPDGTQYVRYDQLSDFLDVLEPPLQIHKPNRYKIISMDIPICRGDMMFCVDILDALTKDFFARKGNPIEETAELGEVQQRPDEVGYEPVSSTLWRQREEYCARLIQHAWKRYKQRHGGGTDASGDDLEIDACDNGCGGGNGNENDDSGDGATGSGDNGSQHGGGSISGGGGTPGGGKSKGIIGSTQANIGIVDSNISPKESPDSIGDPQGRQTAVLVESDGFVTKNGHRVVIHSRSPSITSRTADV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VK17</accession>
    <name>A0A6E8VK17_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phosphatidylcholine transfer protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">START domain-containing protein 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">StAR-related lipid transfer protein 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">Ngousso</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="8">Interacts with ACOT13/THEM2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_049463879.1">
        <property type="nucleotide sequence ID" value="XM_049607922.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VK17"/>
    <dbReference type="EnsemblMetazoa" id="ACON004792-RA">
        <property type="protein sequence ID" value="ACON004792-PA"/>
        <property type="gene ID" value="ACON004792"/>
    </dbReference>
    <dbReference type="GeneID" id="120961530"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007048"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004792"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033933"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08911">
        <property type="entry name" value="START_STARD7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.530.20:FF:000017">
        <property type="entry name" value="Phosphatidylcholine transfer protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002913">
        <property type="entry name" value="START_lipid-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051213">
        <property type="entry name" value="START_lipid_transfer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041949">
        <property type="entry name" value="START_STARD7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19308">
        <property type="entry name" value="PHOSPHATIDYLCHOLINE TRANSFER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19308:SF8">
        <property type="entry name" value="STAR-RELATED LIPID TRANSFER PROTEIN 7, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01852">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00234">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50848">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="7" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="START" evidence="13">
        <location>
            <begin position="165"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="391"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="399"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00063535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00069061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00077188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00079049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004792-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="410" mass="48925" checksum="CA803D64108A3BDC" modified="2020-08-12" version="1">MALKLIPNSTLALRKFNKYLRRNVKDQSASILRLWATQCEFVFAQQLRRSQQIFAHYAKIWEERALRELLKRFRGQVQRHAKGFVLSSAALLTFDWERERIELESVQEHMDDFQFLERLQLETIYCKQCRKRKQIDCRVEGVNYCCCPKNVTEMLSAEQNVYTVWEPYIEQKRMITWRQEIKPGLFAYKVYVEYPDVTAEDFLHVQIDVEYRKKWDNTAVNLEVIDEDTAKGSNSHIIYWEALWPKLFANRDYVYNRRFFIDRSRRVIMIVNKSVEHPKCPAKPHTQRVHEYWSYMVIKPTTAFNKPGVSFVLTYFDNPGLSIPKYITTWVAKKQMPDFLRQLHQATVNYAKAKKQNETRIIEFWDKHKDPGFEYPPDTLLGYSAEDFTEAQKQEEESSLQQDFPTVSCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VK18</accession>
    <name>A0A6E8VK18_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Sodium channel protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="22">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 18">Cell membrane</location>
            <topology evidence="1 18">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the sodium channel (TC 1.A.1.10) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON004707-RG">
        <property type="protein sequence ID" value="ACON004707-PG"/>
        <property type="gene ID" value="ACON004707"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002271"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004707"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035906"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001518">
        <property type="term" value="C:voltage-gated sodium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005248">
        <property type="term" value="F:voltage-gated sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0086010">
        <property type="term" value="P:membrane depolarization during action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019228">
        <property type="term" value="P:neuronal action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13433">
        <property type="entry name" value="Na_channel_gate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000061">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000046">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000047">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000019">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000023">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000026">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058542">
        <property type="entry name" value="IQ_SCN5A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001696">
        <property type="entry name" value="Na_channel_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044564">
        <property type="entry name" value="Na_chnl_inactivation_gate"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010526">
        <property type="entry name" value="Na_trans_assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024583">
        <property type="entry name" value="Na_trans_cytopl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043203">
        <property type="entry name" value="VGCC_Ca_Na"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10037:SF288">
        <property type="entry name" value="SODIUM CHANNEL PROTEIN PARA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10037">
        <property type="entry name" value="VOLTAGE-GATED CATION CHANNEL CALCIUM AND SODIUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24609">
        <property type="entry name" value="IQ_SCN5A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06512">
        <property type="entry name" value="Na_trans_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11933">
        <property type="entry name" value="Na_trans_cytopl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00170">
        <property type="entry name" value="NACHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="17 18" id="KW-0407">Ion channel</keyword>
    <keyword evidence="12 18" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0691">RNA editing</keyword>
    <keyword evidence="11 18" id="KW-0915">Sodium</keyword>
    <keyword evidence="3 18" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="16 18" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="7 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 18" id="KW-0813">Transport</keyword>
    <keyword evidence="9 18" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="133"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="219"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="350"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="719"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="763"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="792"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="847"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="927"/>
            <end position="948"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1237"/>
            <end position="1259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1265"/>
            <end position="1291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1312"/>
            <end position="1343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1430"/>
            <end position="1456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1519"/>
            <end position="1537"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1549"/>
            <end position="1568"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1580"/>
            <end position="1599"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1643"/>
            <end position="1666"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1736"/>
            <end position="1756"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="21">
        <location>
            <begin position="1773"/>
            <end position="1808"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="34"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1068"/>
            <end position="1138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1923"/>
            <end position="1998"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="19">
        <location>
            <begin position="376"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="34"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="20">
        <location>
            <begin position="52"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="1095"/>
            <end position="1121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="20">
        <location>
            <begin position="1122"/>
            <end position="1138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="20">
        <location>
            <begin position="1935"/>
            <end position="1963"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU361132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004707-PG"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="2031" mass="227975" checksum="138EC004E2916FEF" modified="2020-08-12" version="1">MTEDSDSISEEERSLFRPFTRESLQAIEARIADEEAKQRELERKRAEGEIRYDDEDEDEGPQPDPTLEQGVPVPVRMQGSFPPELASTPLEDIDSFYSNQRTFVVISKGKDIFRFSATNALYVLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKEFPLDGSWGNLTDESWELFNSNDTNWFYSESGDIPLCGNSSGAGQCDEGYICLQGYGKNPNYGYTSFDTFGWAFLSAFRLMTQDYWENLYQLVLRSAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEALREAEEAAAAKAAKLEAQQAAAAAAANPEIAKSPSDFSCHSYELFVGQEKGNDDNNKEKMSIRSEGLESVSEITRTTAPTATAAGTAKARKVSAFTIRNGRGRFVGVPGSDRKPLVLSTYLDAQEHLPYADDSNAVTPMSEENGAIIVPVYYANLGSRHSSYTSHQSRISYTSHGDLLGGMTKESRLRNRSARNTNHSIVPPPNANNLSYAETNHKGQRDFDLTQDCTDDAGKIKHNDNPFIEPAQTQTVVDMKDVMVLNDIIEQAAGRHSRASDHGVSVYYFPTEDDDEDGPTFKDKALEFLMKMIDIFCVWDCCWVWLKFQEWVAFIVFDPFVELFITLCIVVNTLFMALDHHDMDPDMEKALKSGNYFFTATFAIEATMKLIAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYVDNVDRFPDHDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMLVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNETNKIAEAFNRISRFSNWIKMNLANALKFVKNKLTSQIASVQPTGKGVCPCISSEHGENELELTPDDILADGLLKKGIKEHNQLEVAIGDGMEFTIHGDLKNKAKKNKQIMNNSKDDDTASIKSYGSHKNRPFKDESHKGSAETMEGEEKRDASKEDLGIDEELDDEGEGDEGPLDGELIIHAEEDEVIEDSPADCCPDNCYKKFPVLAGDDDAPFWQGWGNLRLKTFQLIENKYFETAVITMILLSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFIIVMVSLINFVASLCGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCVDKNKTTLPHEIIPDVNACKAENYSWENSPMNFDHVGKAYLCLFQVATFKGWIQIMNDAIDSRDVGKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTNKKFDMIIMLFIGFNMLTMTLDHYKQSETFSAVLDYLNMIFICIFSSECLMKIFALRYHYFIEPWNLFDFVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKDKSGLDDVYNFKTFGQSMILLFQMSTSAGWDGVLDGIINEEDCLPPDNDKGYPGNCGSSTIGITYLLAYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPDGTQYVRYDQLSDFLDVLEPPLQIHKPNRYKIISMDIPICRGDMMFCVDILDALTKDFFARKGNPIEETAELGEVQQRPDEVGYEPVSSTLWRQREEYCARLIQHAWKRYKQRHGGGTDASGDDLEIDACDNGCGGGNGNENDDSGDGATGSGDNGSQHGGGSISGGGGTPGGGKSKGIIGSTQANIGIVDSNISPKESPDSIGDPQGRQTAVLVESDGFVTKNGHRVVIHSRSPSITSRTADV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VK23</accession>
    <name>A0A6E8VK23_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Sodium channel protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="22">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 18">Cell membrane</location>
            <topology evidence="1 18">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the sodium channel (TC 1.A.1.10) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON004707-RL">
        <property type="protein sequence ID" value="ACON004707-PL"/>
        <property type="gene ID" value="ACON004707"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010356"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004707"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034190"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001518">
        <property type="term" value="C:voltage-gated sodium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005248">
        <property type="term" value="F:voltage-gated sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0086010">
        <property type="term" value="P:membrane depolarization during action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019228">
        <property type="term" value="P:neuronal action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13433">
        <property type="entry name" value="Na_channel_gate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000061">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000046">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000047">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000019">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000022">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000023">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000026">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058542">
        <property type="entry name" value="IQ_SCN5A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001696">
        <property type="entry name" value="Na_channel_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044564">
        <property type="entry name" value="Na_chnl_inactivation_gate"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010526">
        <property type="entry name" value="Na_trans_assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024583">
        <property type="entry name" value="Na_trans_cytopl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043203">
        <property type="entry name" value="VGCC_Ca_Na"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10037:SF288">
        <property type="entry name" value="SODIUM CHANNEL PROTEIN PARA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10037">
        <property type="entry name" value="VOLTAGE-GATED CATION CHANNEL CALCIUM AND SODIUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24609">
        <property type="entry name" value="IQ_SCN5A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06512">
        <property type="entry name" value="Na_trans_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11933">
        <property type="entry name" value="Na_trans_cytopl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00170">
        <property type="entry name" value="NACHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="17 18" id="KW-0407">Ion channel</keyword>
    <keyword evidence="12 18" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0691">RNA editing</keyword>
    <keyword evidence="11 18" id="KW-0915">Sodium</keyword>
    <keyword evidence="3 18" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="16 18" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="7 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 18" id="KW-0813">Transport</keyword>
    <keyword evidence="9 18" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
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            <dbReference type="PROSITE" id="PS50222"/>
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            <dbReference type="EnsemblMetazoa" id="ACON004707-PL"/>
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    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
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    <sequence length="2092" mass="234689" checksum="2940AE37086CD107" modified="2020-08-12" version="1">MTEDSDSISEEERSLFRPFTRESLQAIEARIADEEAKQRELERKRAEGEDEDEGPQPDPTLEQGVPVPVRMQGSFPPELASTPLEDIDSFYSNQRTFVVISKGKDIFRFSATNALYVLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILQPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKEFPLDGSWGNLTDESWELFNSNDTNWFYSESGDIPLCGNSSGAGQCDEGYICLQGYGKNPNYGYTSFDTFGWAFLSAFRLMTQDYWENLYQLVLRSAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEALREAEEAAAAKAAKLEAQQAAAAAAANPEIAKSPSDFSCHSYELFVGQEKGNDDNNKEKMSIRSEGLESVSEITRTTAPTATAAGTAKARKVSAASLSLPGSPFNLRRGSRGSHQFTIRNGRGRFVGVPGSDRKPLVLSTYLDAQEHLPYADDSNAVTPMSEENGAIIVPVYYANLGSRHSSYTSHQSRISYTSHGDLLGGMTKESRLRNRSARNTNHSIVPPPNANNLSYAETNHKGQRDFDLTQDCTDDAGKIKHNDNPFIEPAQTQTVVDMKDVMVLNDIIEQAAGRHSRASDHGVSVYYFPTEDDDEDGPTFKDKALEFLMKMIDIFCVWDCCWVWLKFQEWVAFIVFDPFVELFITLCIVVNTLFMALDHHDMDPDMEKALKSGNYFFTATFAIEATMKLIAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYVDNVDRFPDHDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMLVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNETNKIAEAFNRISRFSNWIKMNLANALKFVKNKLTSQIASVQPTGKGVCPCISSEHGENELELTPDDILADGLLKKGIKEHNQLEVAIGDGMEFTIHGDLKNKAKKNKQIMNNSKDDDTASIKSYGSHKNRPFKDESHKGSAETMEGEEKRDASKEDLGIDEELDDEGEGDEGPLDGELIIHAEEDEVIEDSPADCCPDNCYKKFPVLAGDDDAPFWQGWGNLRLKTFQLIENKYFETAVITMILLSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFIIVMVSLINFVASLCGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCVDKNKTTLPHEIIPDVNACKAENYSWENSPMNFDHVGKAYLCLFQVATFKGWIQIMNDAIDSRDVSFVGKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTNKKFDMIIMLFIGFNMLTMTLDHYKQSETFSAVLDYLNMIFICIFSSECLMKIFALRYHYFIEPWNLFDFVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKDKSGLDDVYNFKTFGQSMILLFQMSTSAGWDGVLDGIINEEDCLPPDNDKGYPGNCGSSTIGITYLLAYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPDGTQYVRYDQLSDFLDVLEPPLQIHKPNRYKIISMDIPICRGDMMFCVDILDALTKDFFARKGNPIEETAELGEVQQRPDEVGYEPVSSTLWRQREEYCARLIQHAWKRYKQRHGGGTDASGDDLEIDACDNGCGGGNGNENDDSGDGATGSGDNGSQHGGGSISGGGGTPGGGKSKGIIGSTQANIGIVDSNISPKESPDSIGDPQGRQTAVLVESDGFVTKNGHRVVIHSRSPSITSRTADV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VK38</accession>
    <name>A0A6E8VK38_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Ion_trans_2 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the two pore domain potassium channel (TC 1.A.1.8) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VK38"/>
    <dbReference type="EnsemblMetazoa" id="ACON004720-RB">
        <property type="protein sequence ID" value="ACON004720-PB"/>
        <property type="gene ID" value="ACON004720"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007799"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004720"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038117"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015271">
        <property type="term" value="F:outward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022841">
        <property type="term" value="F:potassium ion leak channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030322">
        <property type="term" value="P:stabilization of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003280">
        <property type="entry name" value="2pore_dom_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11003:SF325">
        <property type="entry name" value="POTASSIUM CHANNEL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11003">
        <property type="entry name" value="POTASSIUM CHANNEL, SUBFAMILY K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01333">
        <property type="entry name" value="2POREKCHANEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5 8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="27"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="175"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="233"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="11">
        <location>
            <begin position="24"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="11">
        <location>
            <begin position="185"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="95"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="97"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF07885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004720-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="289" mass="31441" checksum="069B364948B03F01" modified="2020-08-12" version="1">DTIIRAVRASRTQQVTNVQQNTRPYKWTFASAFLYSLTLITTIGFSGISPRTQWGRVAALIYALFGIPIILLYLSAIGEGLSSGMRCLFRRLRPNRSPSGSTNNSNSSNSSIGSVSVPMSSKATVAELQKRSQQQSYHQSWNHGGTTIHDPCAYALNAHGLRGGMATNSKMHSQSVVPISICIMILICYITLGAVLFHKLQPWGVLESLYFCFTSLGTIGFGDLMPKGTVAQYAASAYIIIGMAVVAMCFSLIQTELIIWLKKFTIPESLPTSTEDVALVSVAMTPIKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VK57</accession>
    <name>A0A6E8VK57_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">Pyruvate carboxylase</fullName>
            <ecNumber evidence="3 11">6.4.1.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>hydrogencarbonate + pyruvate + ATP = oxaloacetate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20844"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.4.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>biotin</name>
            <dbReference type="ChEBI" id="CHEBI:57586"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <dbReference type="EC" id="6.4.1.1" evidence="3 11"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8VK57"/>
    <dbReference type="EnsemblMetazoa" id="ACON004742-RB">
        <property type="protein sequence ID" value="ACON004742-PB"/>
        <property type="gene ID" value="ACON004742"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004513"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004742"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034193"/>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009374">
        <property type="term" value="F:biotin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004736">
        <property type="term" value="F:pyruvate carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd06850">
        <property type="entry name" value="biotinyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07937">
        <property type="entry name" value="DRE_TIM_PC_TC_5S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000003">
        <property type="entry name" value="Acetyl-CoA carboxylase biotin carboxyl carrier protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000018">
        <property type="entry name" value="Biotin carboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000010">
        <property type="entry name" value="Propionyl-CoA carboxylase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000033">
        <property type="entry name" value="Pyruvate carboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000012">
        <property type="entry name" value="Pyruvate carboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.600.10">
        <property type="entry name" value="pyruvate carboxylase f1077a mutant domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005481">
        <property type="entry name" value="BC-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001882">
        <property type="entry name" value="Biotin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011764">
        <property type="entry name" value="Biotin_carboxylation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005482">
        <property type="entry name" value="Biotin_COase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003379">
        <property type="entry name" value="Carboxylase_cons_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CPAse_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055268">
        <property type="entry name" value="PCB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005930">
        <property type="entry name" value="Pyruv_COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006761">
        <property type="entry name" value="PRK09282.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009554">
        <property type="entry name" value="PRK12999.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01235">
        <property type="entry name" value="pyruv_carbox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43778">
        <property type="entry name" value="PYRUVATE CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43778:SF2">
        <property type="entry name" value="PYRUVATE CARBOXYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02785">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00289">
        <property type="entry name" value="Biotin_carb_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02436">
        <property type="entry name" value="PYC_OADA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001594">
        <property type="entry name" value="Pyruv_carbox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00878">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89000">
        <property type="entry name" value="post-HMGL domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="Rudiment single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50979">
        <property type="entry name" value="BC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00188">
        <property type="entry name" value="BIOTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 11 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 11" id="KW-0092">Biotin</keyword>
    <keyword evidence="4" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="5 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="7 11 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Biotin carboxylation" evidence="18">
        <location>
            <begin position="37"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="17">
        <location>
            <begin position="157"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="19">
        <location>
            <begin position="565"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="domain" description="Lipoyl-binding" evidence="16">
        <location>
            <begin position="1105"/>
            <end position="1180"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="329"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="237"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="272"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="574"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="646"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="via carbamate group" evidence="14">
        <location>
            <position position="743"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="773"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="775"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="910"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-carboxylysine" evidence="15">
        <location>
            <position position="743"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-biotinyllysine" evidence="15">
        <location>
            <position position="1146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR001594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR001594-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR001594-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR001594-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR001594-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004742-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1180" mass="130512" checksum="73408BF2395952D5" modified="2020-08-12" version="1">MQLFSAQLAVAGLRRTNRARLWTIRKNGFASQVEYKPIRSVLVANRGEIAIRVFRACTELGIRSVAVYSEQDKMHMHRQKADESYMVGKGLAPVEAYLSIPEIIRVCKENDVDAVHPGYGFLSERSDFAQAVIDAGLRFIGPSPKVVQQMGDKVAARKAAIEAGVPIVPGTDGPVTTKEEAVEFCRKHGLPVIFKAAYGGGGRGMRVVRKMDEVEDNFMRASSEAKAAFGNGAMFIEKFIERPRHIEVQLLGDKAGNVVHLYERDCSVQRRHQKVVEIAPAPRLPIEVRDKMTEYAVRLARHVGYENAGTVEFLCDESGNFYFIEVNARLQVEHTVTEEITGIDLVQSQIRVAEGMTLPELGYTQENIKPQGYAIQCRVTTEDPANDFQPNTGRLEVFRSGEGMGIRLDSASAYAGAIISPYYDSLLVKVISHASDLQSSAAKMNRALREFRIRGVKTNIPFLLNVLENQKFLNGVLDTYFIDEHPQLFRFSKSKNRAQKLLNYLGEVLVNGPTTPLATKLKPAEVQPHVPQLPLVTEPPRGFKQILQEEGPEGFAKAVRSQKKLLLMDTTFRDAHQSLLATRVRTHDLLKISPFVAHKFNNLYSLENWGGATFDVALRFLHECPWERLEEMRKQIPNIPFQMLLRGANAVGYTNYPDNVVYKFCELSVQCGMDIFRVFDSLNYLPNLILGMEAAGNAGGVVEAAISYTGDVSDPKKTKYDLKYYTNLADELVKAGTHVLCIKDMAGLLKPQAARLLITAIREKHPDVPIHIHTHDTSGAGVASMLACAEAGADVVDVAVDSMSGMTSQPSMGAVVASLQGTSLDTGLGLPDISEYSAYWEQTRTLYAPFECTTTMKSGNADVYMNEIPGGQYTNLQFQAYSLGLGDFFEDVKKAYREANLLLGDIIKVTPSSKVVGDLAQFMVQNHLTADQVLERAEELSFPKSVVEFLQGAIGTPHGGFPEPLRSRVLKDMPRIEGRPGAQLAPLDFDALKKSLQESHPDVQDRDVMSAALYPQVTNDFLNFRDTFGPVDKLNTRVFLTGPKVGEEFEATIEKGKTLGFKTLAMAEDLTANGEREVFFELNGQLRSVLIRDKEAVKELHIHPKATKGNKNEVGAPMPGSVIEIKVKVGDHVEKGQPLIVLSAMKMEMVVQSPRAGVVKTLDISNGMKLEGEDLLLTLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VK61</accession>
    <name>A0A6E8VK61_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Endophilin-A</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">SH3 domain-containing GRB2-like protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required presynaptically at the neuromuscular junction. Implicated in synaptic vesicle endocytosis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the endophilin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VK61"/>
    <dbReference type="EnsemblMetazoa" id="ACON004766-RD">
        <property type="protein sequence ID" value="ACON004766-PD"/>
        <property type="gene ID" value="ACON004766"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012564"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004766"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_043205"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016191">
        <property type="term" value="P:synaptic vesicle uncoating"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11803">
        <property type="entry name" value="SH3_Endophilin_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000072">
        <property type="entry name" value="Unconventional Myosin IB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004148">
        <property type="entry name" value="BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035824">
        <property type="entry name" value="Endophilin_A_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050384">
        <property type="entry name" value="Endophilin_SH3RF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14167:SF81">
        <property type="entry name" value="ENDOPHILIN-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14167">
        <property type="entry name" value="SH3 DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03114">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01887">
        <property type="entry name" value="SPECTRNALPHA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51021">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="BAR" evidence="13">
        <location>
            <begin position="1"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="12">
        <location>
            <begin position="177"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="152"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004766-PD"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="240" mass="27350" checksum="BD117B13BB1C69C7" modified="2020-08-12" version="1">MGDSLKQMADVKYSLDDNIKQNFLEPLHQLQTKDLKEVMHHRKKLQGRRLDFDCKKRRQAKDDEIRSAEEKFAESLQLAQVGMYNLLENDVEQVSQLVTFAEGMLDYHSQCSEILKVLVETLNEKREEAAARPKSEFVPKTLADLNIEMLGSQDGMNASPLPSPMRSPARTPMPTKPTQPCCTALYDFEPENPGELGFKENDVITLIQRVDENWFEGSLNGRNGYFPQSYVQVTVPLPSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VK68</accession>
    <name>A0A6E8VK68_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">CARMIL C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CARMIL family.</text>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON004841-RD">
        <property type="protein sequence ID" value="ACON004841-PD"/>
        <property type="gene ID" value="ACON004841"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010900"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004841"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042041"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034315">
        <property type="term" value="P:regulation of Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000009">
        <property type="entry name" value="F-actin-uncapping protein LRRC16A isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031943">
        <property type="entry name" value="CARMIL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041245">
        <property type="entry name" value="CARMIL_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051279">
        <property type="entry name" value="PP1-Reg/Actin-Interact_Protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24112:SF66">
        <property type="entry name" value="LEUCINE-RICH REPEAT, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24112">
        <property type="entry name" value="LEUCINE-RICH REPEAT, ISOFORM F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17888">
        <property type="entry name" value="Carm_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16000">
        <property type="entry name" value="CARMIL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CARMIL pleckstrin homology" evidence="11">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="CARMIL C-terminal" evidence="10">
        <location>
            <begin position="746"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="915"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1018"/>
            <end position="1039"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1064"/>
            <end position="1083"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1089"/>
            <end position="1175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1250"/>
            <end position="1326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="915"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1115"/>
            <end position="1125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1260"/>
            <end position="1270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1271"/>
            <end position="1292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1293"/>
            <end position="1311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004841-PD"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1373" mass="149872" checksum="FA18B34E9EB16F54" modified="2020-08-12" version="1">MVKLEVKSDKTENRVLVFTPVRVYLLTAKVPTRIDCHFHYLDIQSIESRKPTHFTITTNDKSYSFSTIGDAGSFTSNADVILTDLSSAIKQIFPTVPLRYIIRKIDVNPIERTSIFSDELRPSDPRNVGPCGGFSMQYACMCDLHAVQYREEVAWDIDTIYLSHDARVLNLRDFDHLEQKDLMAIVAALEYNTFFRGLKASHTRLSTETLERVLQVLRRSLWLEELHLESLGLKSDFIHKLAVAVISNSAPALRSIDLSHNVIEDKGATHLAGPIAKMSKGFSKLALAHCGLTAKGVNQLAHSLSLNQNISNSLTYLDLSGNILKDDGNNLYNFLAQPNVIEHLDISRTDTTLESVFGALLRGCATHLLHLNVSHNNFGTKKGKEIPPSFKQFFTSSLSLKHLNIASCKLPLEALKHLLLGLACNESTAGLYLDLSGNSLGSQGAHVLESCIHGVRVLACLDISDNNMDYELANVLTAIGKNPSIRSLYMSKCFTGMKLKHIGTVMDSLVNLIQKEDFQLQELVLAENRLKTDLHNFINALGSNQHLQMLDITGNLMGDIGARLLAKALQINNKLKTIALDRNNFTLQGYADIVYALENNFSVRNMPFPLHDIAPCLKQHPERTDVLMKKMQELLFRNGNGFKRTHGQGFRLQHGFLLSSTHQVLDKLVAETQETMSSRQSSLDGSLSRLLEDAENCKQLLPKLQESVRCDPHPIEVRLGKMKTELHHSIKSYLEETMETMLRTGIEQCPKTLGSQVVLTELRKGCEERLTISEEFLQSCLVNSAGGEVMNRISEVEQSLASLISDRATDEVLEALTRYRKGLGIAEGPLSYTDDLHSLDGSKHNSSQSTEPIANFELRGLDLPKLGLDSPTATPQLQHKRRSIARKVRPQSVVGLGIENLSLANIPDVTETSMASVSRSSSNNSGSKKYESSNAKEDECCDSITELPSQSLTLQHLVKGRPKRAKTRAPKKPVITDTVTAGGTVVNEGIESFFRPGSATPSTLTPLVSPTSEECSSLSFVDSPTLSRDDGGRFSNVTSGETTPILEERKPLKLDRSSPLLKGVNWTSRSRSSDNLEKVSPSVNRKSPLVKAATEVVETPVRESRPVDNGAATPITGSKSPSNESVRNHHVESAHGASRLGNGTGQKTPIITPKPRPWSVLGHESKNEFNEPSVDSIDTDGDLVLISGQTPGGSIVGITPGAISSAGVTGGSILGITPGAVIEKKSVRDMAAGLNKLGVELERNSLMRSSISSHSASAVMRTSPSSILGRSSQKNNSMTSASSSSSSSPTSSVKNGDSTTSDGAPTISSGANIEETGAISVPQKDEKHVKRIVAGKKITALFEETLVEGLQKSSRKTMYRESTVYKDEDTVDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VLT0</accession>
    <name>A0A6E8VLT0_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="13">BTB domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Putative transcription factor required for axon growth and guidance in the central and peripheral nervous systems. Repels CNS axons away from the midline by promoting the expression of the midline repellent sli and its receptor robo.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VLT0"/>
    <dbReference type="EnsemblMetazoa" id="ACON005245-RH">
        <property type="protein sequence ID" value="ACON005245-PH"/>
        <property type="gene ID" value="ACON005245"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009842"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005245"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_029613"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016199">
        <property type="term" value="P:axon midline choice point recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048813">
        <property type="term" value="P:dendrite morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008406">
        <property type="term" value="P:gonad development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035167">
        <property type="term" value="P:larval lymph gland hemopoiesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007526">
        <property type="term" value="P:larval somatic muscle development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045476">
        <property type="term" value="P:nurse cell apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007464">
        <property type="term" value="P:R3/R4 cell fate commitment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045467">
        <property type="term" value="P:R7 cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18315">
        <property type="entry name" value="BTB_POZ_BAB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000091">
        <property type="entry name" value="Lola, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051095">
        <property type="entry name" value="Dros_DevTransReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110">
        <property type="entry name" value="BTB DOMAIN TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110:SF111">
        <property type="entry name" value="LONGITUDINALS LACKING PROTEIN, ISOFORMS F_I_K_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3" id="KW-0221">Differentiation</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="11">
        <location>
            <begin position="32"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="544"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="115"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="301"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="506"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="161"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="175"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="212"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="322"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="515"/>
            <end position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005245-PH"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="601" mass="63236" checksum="481644AE6222D492" modified="2020-08-12" version="1">MDDDQQFCLRWNNHQSTLISVFDTLLENGTLVDCTLAAEGKLLKAHKVVLSACSPYFATILSQQYDKHPIFILKDVKFQELRAMMDYMYRGEVNISQDQLAALLKAAESLQIKGLSDNRSSSSSGTAAAGGGVHQKQSDAPAAKTLQPPVPASKASGLTIENKRSLKSELLESDVSGSREGSTSPTSRKRKKVRRRSVDTNNLLIDNHDQHSNSSSHSMHTAGGANVAAAVAAAGAPAANVASSLSSASSTAAAGSNTSANVLSVAAVAAAAVLKKTETVQQAAAETLKLQKQQAAAAAAAAAAQSSDDEERCGPGSVATGTEHDDGNDLSDNEHDGDGGSSVKSGSRHHHLRDGSRRGKTSTSSMGEMMIEPKSEYEDVQDENVEDLTMDDEDLMDELDQAGPSHGGDVSTQPKVQRTIATTTTTIPAWRCKTSKSTSKISLAPMVTTVGRMMVAKKSSPSGGGVGGGASMLAIMKPLKTTGNGNVILTSAGSLMATTISSGTNATGGGGGSGSASSNGSSISLGSRKEDKIMFQHENDAKPWKCKSCGRNYKWKNSLKCHIKNECGVPPKYFCERMCGYKTHIHSNLKRHLNSKFCKPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VLT4</accession>
    <name>A0A6E8VLT4_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="7">SERPIN domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the serpin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VLT4"/>
    <dbReference type="EnsemblMetazoa" id="ACON005246-RA">
        <property type="protein sequence ID" value="ACON005246-PA"/>
        <property type="gene ID" value="ACON005246"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011585"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005246"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038865"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19601">
        <property type="entry name" value="serpin42Da-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.497.10:FF:000006">
        <property type="entry name" value="Plasminogen activator inhibitor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.39.10">
        <property type="entry name" value="Alpha-1-antitrypsin, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.497.10">
        <property type="entry name" value="Antithrombin, subunit I, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023795">
        <property type="entry name" value="Serpin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023796">
        <property type="entry name" value="Serpin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000215">
        <property type="entry name" value="Serpin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036186">
        <property type="entry name" value="Serpin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042178">
        <property type="entry name" value="Serpin_sf_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042185">
        <property type="entry name" value="Serpin_sf_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461:SF211">
        <property type="entry name" value="GH10112P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461">
        <property type="entry name" value="SERINE PROTEASE INHIBITOR, SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00079">
        <property type="entry name" value="Serpin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00093">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56574">
        <property type="entry name" value="Serpins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00284">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0722">Serine protease inhibitor</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Serpin" evidence="6">
        <location>
            <begin position="21"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005246-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="379" mass="42129" checksum="5BA3FB7D9E251A19" modified="2020-08-12" version="1">MADNSSSLDAQFVSQSNSFATKLYQRVSAKHAGENVVISPFSISACLSLAAMGAGGLTAEQMYSVLEFGAPDRKQTVADNYRRLMERLATDSTVNVANKIYVMQNYAVKGAFNAIATGSFRSEAESVNFAESAAAAKKINGWVEEKTNNKIKDLISPDALDELSRMVLVNAVHFKGTWTYQFDPSLTRPFPFWLSETESRDVPMMNIKKHFAFNNFEELGFSALELTYGGSDMTMMLLLPNERMGLAALEEKLPTLNLAELAGKMHKQEVEVFLPKFKIEFTRDLNEDLQALGMERMFSDSAEFPDLLEQNEPLKVSKVVHKAFIEVNEEGTEAAAATGMVMMTCAMIMYPMFTADHPFLYALKDSQGNILFIGKILKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VLT5</accession>
    <name>A0A6E8VLT5_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Guanine nucleotide-binding protein subunit alpha</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the G-alpha family. G(q) subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VLT5"/>
    <dbReference type="EnsemblMetazoa" id="ACON005079-RC">
        <property type="protein sequence ID" value="ACON005079-PC"/>
        <property type="gene ID" value="ACON005079"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007505"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005079"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032935"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005834">
        <property type="term" value="C:heterotrimeric G-protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001664">
        <property type="term" value="F:G protein-coupled receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031683">
        <property type="term" value="F:G-protein beta/gamma-subunit complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007188">
        <property type="term" value="P:adenylate cyclase-modulating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050793">
        <property type="term" value="P:regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00066">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.400.10:FF:000002">
        <property type="entry name" value="guanine nucleotide-binding protein G(Q) subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003977">
        <property type="entry name" value="Guanine nucleotide-binding protein G(q) subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000692">
        <property type="entry name" value="Guanine nucleotide-binding protein subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.400.10">
        <property type="entry name" value="GI Alpha 1, domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000654">
        <property type="entry name" value="Gprotein_alpha_Q"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001019">
        <property type="entry name" value="Gprotein_alpha_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011025">
        <property type="entry name" value="GproteinA_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218">
        <property type="entry name" value="GTP-BINDING PROTEIN ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218:SF329">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN G(Q) SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00503">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00318">
        <property type="entry name" value="GPROTEINA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00442">
        <property type="entry name" value="GPROTEINAQ"/>
    </dbReference>
    <dbReference type="SMART" id="SM00275">
        <property type="entry name" value="G_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47895">
        <property type="entry name" value="Transducin (alpha subunit), insertion domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51882">
        <property type="entry name" value="G_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9 11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4 10 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 9 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0564">Palmitate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0807">Transducer</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="43"/>
            <end position="48"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="149"/>
            <end position="150"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="174"/>
            <end position="180"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="199"/>
            <end position="203"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="268"/>
            <end position="271"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="325"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU369122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005079-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="353" mass="41601" checksum="F8297FA98E90D36E" modified="2020-08-12" version="1">MECCLSEEAKEQKRINQEIERQLRRDKRDARRELKLLLLGTGESGKSTFIKQMRIIHGSGYSDEDKRGFIKLVYQNIFMAMQSMIRAMDLLKILYSDPANIEHAELIRSVDFETVTTFEPPYVQAIKDLWADAGIQECYDRRREYQLTDSAKYYLMEIDRVAAPNYLPTEQDILRVRVPTTGIIEYPFDLEEIRFRMVDVGGQRSERRKWIHCFENVTSIIFLVALSEYDQILFESENENRMEESKALFKTIITYPWFQHSSVILFLNKKDLLEEKIMYSHLVDYFPEYDGPQRDAIAAREFILRMFVDLNPDSEKIIYSHFTCATDTENIKLVFCAVKDTIMQTALKEFNLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VLV1</accession>
    <name>A0A6E8VLV1_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Macro domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VLV1"/>
    <dbReference type="EnsemblMetazoa" id="ACON005091-RC">
        <property type="protein sequence ID" value="ACON005091-PC"/>
        <property type="gene ID" value="ACON005091"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_013305"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005091"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032702"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48411">
        <property type="entry name" value="OS01G0948300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48411:SF1">
        <property type="entry name" value="OS01G0948300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13716">
        <property type="entry name" value="CRAL_TRIO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="1">
        <location>
            <begin position="95"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005091-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="268" mass="30509" checksum="EFB3EE8EE04FE98A" modified="2020-08-12" version="1">MIEADDDSDTSPHDLEMGSDVEYSNINLSLAAQLSGAAHSFTQMQGDLDRQRLLGDRPRMVYESVLDDGLEGIEHQESSPLLVDCEYERLLRRAKTEDLSEVSGIGCLYQSGVDRLGRPVVVFCGKWFPAHNIDLEKALLYLIYLLDPIVKGDYVIAYFHTLTSSNNYPSLQWLKDVYSILPYKYKKNLKAFYIVHPTFWTKMMTWWFTTFMAPAIKTKVHSLPGVEHLYSAIAKDQLEIPAYITEYDMATNGIHYFQPITNTSSPAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VLW5</accession>
    <name>A0A6E8VLW5_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Srp40 C-terminal domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="7">Ngousso</strain>
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        <property type="gene ID" value="ACON005369"/>
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    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
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        <property type="entry name" value="Znf_RanBP2_sf"/>
    </dbReference>
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        <property type="entry name" value="NUCLEOLAR AND COILED-BODY PHOSPHOPROTEIN 1"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Zn_ribbon_RanBP"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="ZnF_RBZ"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Ran binding protein zinc finger-like"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RanBP2-type" evidence="6">
        <location>
            <begin position="298"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="RanBP2-type" evidence="6">
        <location>
            <begin position="328"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
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            <begin position="53"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="350"/>
            <end position="452"/>
        </location>
    </feature>
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        <location>
            <begin position="69"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="95"/>
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        </location>
    </feature>
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    </feature>
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        </location>
    </feature>
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        <location>
            <begin position="193"/>
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        </location>
    </feature>
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        <location>
            <begin position="213"/>
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        </location>
    </feature>
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        <source>
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        <source>
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        <source>
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        </source>
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        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005369-PA"/>
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    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="452" mass="48558" checksum="C45691E76483CC4D" modified="2020-08-12" version="1">MAALADSVLDALVYEHLSKKDKTFAAVFQKKFQSPVLPKGAPKLDEIVKHYQSTKKKLVVNSQQKAEESDSDEEEEDSDEEEDEEETPAPVKKPATNGKAAVNGKAATNGAAAKKPQPEEDDDEEEDSDDDDEEEDSDEEEEEDEKPAAKPAAKATPAKVTPVVKKPQQEEESDDEDDDEDEDDSDEEEEAAPKPQQQKPAAQQAGQKRKAQEEEEEDEDDDDDEDDDEEEEEEETPKPVLQKANNAAKHQQKDQKNDNRKSFGGFDNRNGNSGGGYNKQQGGNNNDQYGEKKPYESKPGDWECDACGANNFRTRRNCFKCSQENPNEPDTWSCPNCQFDNFPSRWSCFKCQTDNPNGGGGGGRGGGRGGNRRSFGEGGGRGGGEGGRGGRGGGGFRGGAGGRGGGGRGGGGFRGGRGGQGGGGRGGRGSFNGNRSFDGAPPPNKKMTFGDD</sequence>
</entry>
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        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
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            <taxon>Insecta</taxon>
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            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
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            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">Ngousso</strain>
        </source>
    </reference>
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        <property type="gene ID" value="ACON005378"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="CALML5 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Uncharacterized protein, isoform A"/>
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    </dbReference>
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        <property type="entry name" value="EF-hand-dom_pair"/>
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        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
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    <keyword evidence="2" id="KW-0677">Repeat</keyword>
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            <end position="70"/>
        </location>
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            <end position="200"/>
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    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005378-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="207" mass="23309" checksum="390AE81736A4F3B5" modified="2020-08-12" version="1">MSDFSEIEDAAKMLGAALIGRRKSSVAKGKTFTEEEIKDLRTAFDLLDRDQDGHVTPEELQFMLRNLGIHVRDELIDDLLREASRTGSGLIDETEFLQWVARIQALKDDSNTSSSSSSSNNPAQAADDDLTQDLVAAFRVFDRDGNGYITRDELKSAMDMIGENVTEYQLNEMLELADADKDGRINYEDFQNFFLNNFDALVQPPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VLX6</accession>
    <name>A0A6E8VLX6_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Tubulin beta chain</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12 13">Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4 13">Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the tubulin family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_040362728.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VLX6"/>
    <dbReference type="EnsemblMetazoa" id="ACON005293-RB">
        <property type="protein sequence ID" value="ACON005293-PB"/>
        <property type="gene ID" value="ACON005293"/>
    </dbReference>
    <dbReference type="GeneID" id="120947410"/>
    <dbReference type="KEGG" id="acoz:120947410"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005105"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005293"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041609"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02187">
        <property type="entry name" value="beta_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.600:FF:000013">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <sequence length="449" mass="50766" checksum="95DE90422E2DF1D0" modified="2020-08-12" version="1">MREIVHLQAGQCGNQIGSKFWEIISDEHGIDPTGHYHGDSDLQLERIDVYYTEVNGNKYVPRAVLVDLEPGTMDSVRQSPYGALFRPDNFVYAQSGAGNNWAKGHYTEGAELVDNVLDVIRKETESCDCLQGFQLAHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSIHQLVENTDDTFCIDNEALYDICFRTLKLSSPTYGDLNHLVSVTMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTAKGSQQYRALTVPELTQQMFDAKNMMTACDPRHGRYLTCAAIFRGSMSMKEVDQQMLNIQSKYSSYFVEWIPNNVKVAVCDIAPRGLKMSATFIGNTTAIQEIFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLISEYQQYQDAEVEDYDEMEEIPEEEQQQQQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Anopheles coluzzii</name>
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        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
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                <consortium name="EnsemblMetazoa"/>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="2">Ngousso</strain>
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        <property type="gene ID" value="ACON005114"/>
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        <property type="component" value="Unplaced"/>
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        <location>
            <begin position="23"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005114-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="74" mass="8867" checksum="3AC65049C7926D88" modified="2020-08-12" version="1">TRRAIYSEAHGVDCFWYRRRRRVLLPLLLLLWFVVCFVAIATNQTVTSSNPTPSEVCECEQKRVQRWRSLERRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VLY7</accession>
    <name>A0A6E8VLY7_ANOCL</name>
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        <submittedName>
            <fullName evidence="8">BZIP domain-containing protein</fullName>
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        <name type="scientific">Anopheles coluzzii</name>
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        <dbReference type="NCBI Taxonomy" id="1518534"/>
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            <taxon>Anopheles</taxon>
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            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
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                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
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            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
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                <consortium name="EnsemblMetazoa"/>
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            <strain evidence="8">Ngousso</strain>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="TF_DNA-bd_sf"/>
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        <property type="entry name" value="NUCLEAR FACTOR ERYTHROID 2-RELATED FACTOR"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR24411:SF55">
        <property type="entry name" value="SEGMENTATION PROTEIN CAP'N'COLLAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03131">
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        <property type="match status" value="1"/>
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        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="A DNA-binding domain in eukaryotic transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0010">Activator</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="7">
        <location>
            <begin position="439"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="46"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="85"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="171"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="212"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="358"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="538"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="52"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="85"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="97"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="226"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="389"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="544"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="577"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="630"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="650"/>
            <end position="663"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005300-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="680" mass="69973" checksum="F157CE20B3CE45E5" modified="2020-08-12" version="1">MLSPYAFLGMYTMRMLEHNGTTGSGGNNASGGGGMLGVSASAASNLVNHGGVHPHSHHHHHLSLMSSASGGGLQNAMNVSALVNGSSSASSASSGASSGGGAGGASSGTPGDRLDASSDSAVSSMGSERVPSLSDGEWGDGGSDSAQEYHNGKYGGPYDYSYNQTNGAGGGGAGGAGGVPSSRMGEHGPRQGPPVAQKKHHMFAKRYFQEQNTSTVPSIHPSPNGGTAGTAGGPPGLDGGAAGGAAGGGAGATAAGVLLPTAIKYEYDYMNVVGPQTAAGPTIGPLEGAAKQEDHLQHHQHESSAAAAAAAAAALTDMKYPYGIDFPRSHHSAAMGGRGPLQPQDMIHHNHTYTLPFATPNGGGGAVGVHQGPKPQTRDKSRSSALHSRKAEEEHLTRDEKRARALQIPIPVHDIINLPMDEFNERLSKYDLSETQLSLIRDIRRRGKNKVAAQNCRKRKLDQIVTLADEVKDMKMRKERLMRDREMVLSEHKKIRDKFSMLYRHVFQNLRDADGNPYSQEHYSLQQSADGAVVLVPRNSERPNGAGSNHLGGNSSNGTANGVHPSLHHSGAAGTHHAGHHHQHGHGHHQLHPHHHLQHHHGLIGGGSAAMNGSMNGSLAMGGASSNGASVTGSNHSSSSSSTSSSSSGVVGGSGGSVQGSGMNGTASATAAIVHQRTKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VM70</accession>
    <name>A0A6E8VM70_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="3">IMD domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="EnsemblMetazoa" id="ACON005508-RB">
        <property type="protein sequence ID" value="ACON005508-PB"/>
        <property type="gene ID" value="ACON005508"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011830"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005508"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038027"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030031">
        <property type="term" value="P:cell projection assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007009">
        <property type="term" value="P:plasma membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013606">
        <property type="entry name" value="I-BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030127">
        <property type="entry name" value="MTSS1/MTSS2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15708">
        <property type="entry name" value="ACTIN BUNDLING/MISSING IN METASTASIS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15708:SF4">
        <property type="entry name" value="FI21477P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08397">
        <property type="entry name" value="IMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51338">
        <property type="entry name" value="IMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IMD" evidence="2">
        <location>
            <begin position="5"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="288"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="388"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="438"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="559"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="614"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="693"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="779"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="857"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="909"/>
            <end position="948"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="992"/>
            <end position="1171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1227"/>
            <end position="1326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1337"/>
            <end position="1356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="288"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="306"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="318"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="388"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="439"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="485"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="511"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="573"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="614"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="704"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="780"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="790"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="817"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="927"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1005"/>
            <end position="1035"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1037"/>
            <end position="1059"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1067"/>
            <end position="1080"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1089"/>
            <end position="1104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1149"/>
            <end position="1171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1232"/>
            <end position="1246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1266"/>
            <end position="1292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1308"/>
            <end position="1326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005508-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1441" mass="154371" checksum="12F120C85115BBFE" modified="2020-08-12" version="1">MDIGMDRSGTDAGMATIGSLFQHIVNEMKNSSPLWEDFIAKATKLHACLRAAIQALAAYLDAFQKIADAATNSRGATKEIGTALTRVCLRHKAVESRMKTFTTAIMDCLVVPLQEKLEDWKKQVTVIDKDHAKEYKRCRAELKKRSSDTLRLQKKAKKGAADNLHVLVESSMQDVTMRRCELEEVERKSLRAIMVEERTRYCTFVNMLQPVVHEECEVMSELGHLQEAMQLIAIVTKDPAQLPQASEELILESKANISLYPDSPGGSNSQGGCSNSLGSRKSSVCSISSINSSSSGSPGHHQFQRSLSQASTLASQSHAVSTWPPHTQDATSTVDRPHTISSAYEKGHQRPALTVYTFQSPETIAETQKSPANVACRPPLPVRPLSTTSVKNANPNVPRQCPSPIPAHITKGMNEKCRISSPTYVNMTELASMAASKTTNNSNNVNNNNNNNNHHTSTSGNNGGASANNVQQAASLQSHSQIPYQQQQQQQQQLHSPVLSSASSLISPDSNATNAISSPDVSACSTQTPQNTPQTGSPGTPNYSSIGCAGGGTINLGFRSTTPSSAGGGTAAVTPSESNIDTSSNHTITIDDNEEHFNNTISTTSASANITSTTTISTSDTSNNSSSNNTTTNTTTTATSSTNKPSPPHCSTVGGTADAPPKSVLDKDKDILKRTGSVLEKTSLFEQQINNNQLHQPTVPPPQQQQLSVPTSPATLSTRHNDPNALYGRRTNEEIYKSAGQLLLDRDADCIDKQTIEELNNLIGELDLFQREHENALLLQQQQQQQQQQHHPHHPPQKHARHRYSNGGSGSGDPASESDTILANGVNNNHGGGMLASGHRNGSLHDAEDGLQLLEQPTTALGGGGRLPSSISSSNSNLDEYLSNHQAADETNGSMTNLAAKYSNHNAQSADTTDYASTGGHYHRTSAYGQQQHQHQNHSIGLGLGSSAGGSTQGLDGIATGFENPSFMMENYYSQNRSEVVVLRCKDTSRNSLNNAPDDLLTGSGLMQLNGTPVDNSHQQQQQRLSSFRVTTSRPASPASMSSFFGISSRSPTPSLSLPVTANSSPQHHHHHGHNNHHHQTATAEANDPAVAPNSSNHAPSSAGPAPPYDDRINRNKPAITPRPASLSGPTRVTRRASVNTVKPPPPVRRSSSVTPSPSVGTNSTNTTNTTNLAQQSLAYTSSESLPPPPAYLLDSAAGSSPSISGNVAGTVKALNEIRHTPASPGVLRRAQQQSNPPSNQGSPTQYTNQYGTLPANRQHGHDHIASTTSSLPPTAPTAAAVTAPQSHYPPSQHHHHHHPSTPNSFHATDSNKPLSNRSPKTNLHQQGGIYAQPKQLSTMSSFRTSSPGPQKPNSSFLAQLNAKIAPNKTPQSHAGFLENLNARLAEQRLSGKAFAVRNLINSKALPDPRICHESLMDQIKRGATLKRNRTINDRSAPKIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VM78</accession>
    <name>A0A6E8VM78_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Peptidoglycan recognition protein family domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VM78"/>
    <dbReference type="EnsemblMetazoa" id="ACON005203-RD">
        <property type="protein sequence ID" value="ACON005203-PD"/>
        <property type="gene ID" value="ACON005203"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_014474"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005203"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033832"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008745">
        <property type="term" value="F:N-acetylmuramoyl-L-alanine amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009253">
        <property type="term" value="P:peptidoglycan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06583">
        <property type="entry name" value="PGRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.80.10:FF:000001">
        <property type="entry name" value="Peptidoglycan recognition protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.80.10">
        <property type="entry name" value="Peptidoglycan recognition protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036505">
        <property type="entry name" value="Amidase/PGRP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002502">
        <property type="entry name" value="Amidase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015510">
        <property type="entry name" value="PGRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006619">
        <property type="entry name" value="PGRP_domain_met/bac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11022">
        <property type="entry name" value="PEPTIDOGLYCAN RECOGNITION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11022:SF41">
        <property type="entry name" value="PEPTIDOGLYCAN-RECOGNITION PROTEIN LC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01510">
        <property type="entry name" value="Amidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00644">
        <property type="entry name" value="Ami_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00701">
        <property type="entry name" value="PGRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55846">
        <property type="entry name" value="N-acetylmuramoyl-L-alanine amidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0391">Immunity</keyword>
    <keyword evidence="2" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="218"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidoglycan recognition protein family" evidence="7">
        <location>
            <begin position="255"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetylmuramoyl-L-alanine amidase" evidence="6">
        <location>
            <begin position="267"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="171"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="8"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="50"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="171"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005203-PD"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="432" mass="47118" checksum="9DFAD02124CF9F2C" modified="2020-08-12" version="1">MALARNLQLENDTSVNTGYHTKAQSMSASGGDTIAGLNNSGTGTGKSDSAKGSSLSNISASSIKRTDNGGDDDDSSVFDSSSSDTECDDDSIKRAIDRIPGTLAPGEARVLPNANVKVAVEAIAPGVRPSPAASTIGAIAVHNSSDITFGNKTYIKGQVVIKNIYQDRKNGTTNQGYQVQENETDDTNSTKKVPSATKPPEPRTWQSSLKTIIKDKPLLSFIVMVSLMIVLCAIVAVISILTASEDDLFPDPRPLRLVTRTEWLAQPPREELTDLKLPVNNVIIAHTATEGCTTQAACRLRVRLIQEFHMDGKNYDDITYNFLIGGDGHIYEGRNWHKIGATIPGYNSRSITVAFVGEYNYGGKPTKKQIELLKYLLHFGAKERHLKEGYRIYASEQLDPTVGTGKWLIEALHSLPQFVDKEQNKDEQPDHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VM84</accession>
    <name>A0A6E8VM84_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Peptidoglycan recognition protein family domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VM84"/>
    <dbReference type="EnsemblMetazoa" id="ACON005205-RC">
        <property type="protein sequence ID" value="ACON005205-PC"/>
        <property type="gene ID" value="ACON005205"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002089"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005205"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030932"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008745">
        <property type="term" value="F:N-acetylmuramoyl-L-alanine amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009253">
        <property type="term" value="P:peptidoglycan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06583">
        <property type="entry name" value="PGRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.80.10">
        <property type="entry name" value="Peptidoglycan recognition protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036505">
        <property type="entry name" value="Amidase/PGRP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002502">
        <property type="entry name" value="Amidase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015510">
        <property type="entry name" value="PGRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006619">
        <property type="entry name" value="PGRP_domain_met/bac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11022">
        <property type="entry name" value="PEPTIDOGLYCAN RECOGNITION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11022:SF76">
        <property type="entry name" value="PEPTIDOGLYCAN-RECOGNITION PROTEIN LA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01510">
        <property type="entry name" value="Amidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00644">
        <property type="entry name" value="Ami_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00701">
        <property type="entry name" value="PGRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55846">
        <property type="entry name" value="N-acetylmuramoyl-L-alanine amidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0391">Immunity</keyword>
    <keyword evidence="2" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="143"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidoglycan recognition protein family" evidence="7">
        <location>
            <begin position="196"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetylmuramoyl-L-alanine amidase" evidence="6">
        <location>
            <begin position="209"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="48"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="52"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005205-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="404" mass="44821" checksum="C31ED8BC15F2A727" modified="2020-08-12" version="1">MENASASTTGPVPDSVANALMSIHPRYNSFHQHNAFPAAAPVGSLGARYEQNADSPGMATNHQNGLSTGNGGNTTVQPAATSVINLSNSSDVVIGPMTQYQGSVTIYQYMDATVEASRIANGRNNQNRGLPSAEPSTLRQERYFIYGALIFFAIVGFSTAIYFIVNQVRGPADIDREILFDTNYHRRTMPNLGNSHMIIERRNWGSQVDAHASIQLEHPVQYVIVTHIGLRSKNCTGMHECANRMRMLQDAAIGERNLPDIPSNFYLGGDGNVYVGRGWDIANSYHNRTLSVCFIGDFQTYEPKESQFSALHHLLTYGVIQRKLASDYKLVARRQTKPTTASPGDMLYARLVRLSRWNPCGTQAYAHCGAELGFPSVWDDEHDFNERNGIHNPLLIHRKSTEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VM88</accession>
    <name>A0A6E8VM88_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phospholipid scramblase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">May mediate accelerated ATP-independent bidirectional transbilayer migration of phospholipids upon binding calcium ions that results in a loss of phospholipid asymmetry in the plasma membrane.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the phospholipid scramblase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040229889.1">
        <property type="nucleotide sequence ID" value="XM_040373955.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_049463355.1">
        <property type="nucleotide sequence ID" value="XM_049607398.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VM88"/>
    <dbReference type="EnsemblMetazoa" id="ACON005498-RA">
        <property type="protein sequence ID" value="ACON005498-PA"/>
        <property type="gene ID" value="ACON005498"/>
    </dbReference>
    <dbReference type="GeneID" id="120953738"/>
    <dbReference type="KEGG" id="acoz:120953738"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004238"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005498"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030082"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017128">
        <property type="term" value="F:phospholipid scramblase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005552">
        <property type="entry name" value="Scramblase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025659">
        <property type="entry name" value="Tubby-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23248:SF9">
        <property type="entry name" value="PHOSPHOLIPID SCRAMBLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23248">
        <property type="entry name" value="PHOSPHOLIPID SCRAMBLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03803">
        <property type="entry name" value="Scramblase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54518">
        <property type="entry name" value="Tubby C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0564">Palmitate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU363116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005498-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="318" mass="35537" checksum="9C2A0B90D73F71D9" modified="2020-08-12" version="1">MADTPEKVPLEEKQQQPPANPLYPTLGQEAANPLLPQTNPQGYPMAPMATGPPAPPGYMPVPQMAGMPIMTQPGVMQMQPVLGQQQVTGWMPIPQGIPNCPPGLEYLTAVDQLLVHQQVELLEAFTGFETANKYTVKNTLGQKVYWAVEDTTCCTRMCCGPDRPFDIKIMDNFQNEVLHLRRDLRCKSCCFPCCLQKLEVSAPPGNVIGTVVQKWSLCRPVFDIRDRNNETVLTIRGPVCQCPCSDIKFAVFTKDGTEVGKVTKQWTGFVQEHFTDADNFGINFPMDLDVRVKATMLGALFLIDYMYFESSDNKKVRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VM98</accession>
    <name>A0A6E8VM98_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phospholipid scramblase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">May mediate accelerated ATP-independent bidirectional transbilayer migration of phospholipids upon binding calcium ions that results in a loss of phospholipid asymmetry in the plasma membrane.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the phospholipid scramblase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040229883.2">
        <property type="nucleotide sequence ID" value="XM_040373949.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VM98"/>
    <dbReference type="EnsemblMetazoa" id="ACON005504-RB">
        <property type="protein sequence ID" value="ACON005504-PB"/>
        <property type="gene ID" value="ACON005504"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON005504-RC">
        <property type="protein sequence ID" value="ACON005504-PC"/>
        <property type="gene ID" value="ACON005504"/>
    </dbReference>
    <dbReference type="GeneID" id="120953737"/>
    <dbReference type="CTD" id="326186"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004426"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005504"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038722"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017128">
        <property type="term" value="F:phospholipid scramblase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005552">
        <property type="entry name" value="Scramblase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025659">
        <property type="entry name" value="Tubby-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23248:SF9">
        <property type="entry name" value="PHOSPHOLIPID SCRAMBLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23248">
        <property type="entry name" value="PHOSPHOLIPID SCRAMBLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03803">
        <property type="entry name" value="Scramblase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54518">
        <property type="entry name" value="Tubby C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0564">Palmitate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="36"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU363116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005504-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="332" mass="36374" checksum="3DFF3B7FF16E770A" modified="2020-08-12" version="1">MNPQQQPQAPYAPYAPQYQGYTDQPGYPPAGGGGYPPAPYPPPGTGSPYPPAAPGYGQPPQMYAPNQPYGGGGYQPVMVQPGMPGHPPPVMGQPVPGGWMPIPQGIPNCPPGLEYLTAIDQLLVHQKVELLEAFTGFETANKYTVKNTLGQKVYWAMEDTGCCNRMCCGAARAFDMKILDTYQNEVLHFNRPLRCSSCWFPCCLQTMEVTAPPGNVIGYVEQDWSILTPQFSIKNQNGETVLKISGPFCTFSICGDVEFEVLSTNGTQVGKISKQWSGLGREMFTDADHFGINFPMDLDVRVKATLLGALFLIDYMFFEKSGNKEQDRPGMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VNX4</accession>
    <name>A0A6E8VNX4_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Nuclear hormone receptor FTZ-F1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Nuclear receptor subfamily 5 group A member 3</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nuclear hormone receptor family. NR5 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VNX4"/>
    <dbReference type="EnsemblMetazoa" id="ACON005661-RC">
        <property type="protein sequence ID" value="ACON005661-PC"/>
        <property type="gene ID" value="ACON005661"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000880"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005661"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034777"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090575">
        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004879">
        <property type="term" value="F:nuclear receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009755">
        <property type="term" value="P:hormone-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009888">
        <property type="term" value="P:tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07167">
        <property type="entry name" value="NR_DBD_Lrh-1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06944">
        <property type="entry name" value="NR_LBD_Ftz-F1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.565.10:FF:000032">
        <property type="entry name" value="Nuclear hormone receptor FTZ-F1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000006">
        <property type="entry name" value="Nuclear receptor subfamily 5 group A member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.565.10">
        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035500">
        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016355">
        <property type="entry name" value="NR5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000536">
        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001723">
        <property type="entry name" value="Nuclear_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001628">
        <property type="entry name" value="Znf_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24086:SF15">
        <property type="entry name" value="NUCLEAR HORMONE RECEPTOR FTZ-F1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24086">
        <property type="entry name" value="NUCLEAR RECEPTOR SUBFAMILY 5 GROUP A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00104">
        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00105">
        <property type="entry name" value="zf-C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00398">
        <property type="entry name" value="STRDHORMONER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00047">
        <property type="entry name" value="STROIDFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00430">
        <property type="entry name" value="HOLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00399">
        <property type="entry name" value="ZnF_C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48508">
        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51843">
        <property type="entry name" value="NR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00031">
        <property type="entry name" value="NUCLEAR_REC_DBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51030">
        <property type="entry name" value="NUCLEAR_REC_DBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="7 14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10 14" id="KW-0675">Receptor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 14" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 14" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 14" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Nuclear receptor" evidence="16">
        <location>
            <begin position="269"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="NR LBD" evidence="17">
        <location>
            <begin position="548"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="23"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="50"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="202"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="451"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="513"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="15">
        <location>
            <begin position="69"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="88"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="135"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="15">
        <location>
            <begin position="151"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="15">
        <location>
            <begin position="457"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="488"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="528"/>
            <end position="538"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00073670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00078044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005661-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="782" mass="81058" checksum="8EDF0983DBD698E6" modified="2020-08-12" version="1">MLLDMDQQHSTVFMSLNMSQYSMLSESGSPPPGGGGGAGGNTQLLSLAAAASAANSTGQNTGMPPPQYTGGGGGGGGGSGTAGGYPGATGLLLGSSNNNNNNSTSNNNLPSHHNNNNNGAGSNGGHGHHHHHHSQQQQQQHPGSQQQQHLGGAGGGGGNGQSHSQSNGASGGYGALYGGFHESAAAPPSAYSMFSHVPTGGGGGVGGTTGGGPYHHGGSDGGIGGNSNSSSGGGGGGGSQAAGGQTAGGGGSSSAAAAAAAAYLAIPASELCPVCGDKVSGYHYGLLTCESCKGFFKRTVQNKKVYTCVAERQCHIDKTQRKRCPYCRFQKCLEVGMKLEAVRADRMRGGRNKFGPMYKRDRARKLQIMRQRQLALQALRGSIGGGVGIGTLGSDGVSPQLQGLDYHQPYSSMHIKQEIQIPQVSSLTSSPDSSPSPIAIALGQVNPQTTISLSSTGVGGNGGGGGGGGGGGGGGNGGGSNGSGAGTGSSNSNSATNIQNPLSDSKLWMSANSTTASPHSLSPKAFSFDSNPTSTADSGNPADTLRLVVSPMIREFVQSIDDREWQTSLFSLLQSQSYNQCEVDLFELMCKVLDQNLFSQVDWARNTIFFKDLKVDDQMKLLQNSWSDMLVLDHIHQRLHNGLPDETTLHNGQKFDLLGLGLLGVPSLAEHFNELQNKLQELKFDVGDYICMKFLLLLNPDVRGITNRKTVVEGYENVQAALLDYTLTCYPSVPDKFSKLLSIIPEIHGMAARGEEHLYIKHCAGSAPSQTLLMEMLHAKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VNY7</accession>
    <name>A0A6E8VNY7_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Gustatory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Gustatory receptor which mediates acceptance or avoidance behavior, depending on its substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the insect chemoreceptor superfamily. Gustatory receptor (GR) family.</text>
    </comment>
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    </evidence>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain>Mali-NIH</strain>
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        <source>
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        <source>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
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    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
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                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
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        <property type="gene ID" value="ACON006590"/>
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        <property type="component" value="Unplaced"/>
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        <property type="entry name" value="T cell lymphoma invasion and metastasis 1"/>
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        <property type="entry name" value="Dbl homology (DH) domain"/>
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        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
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    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
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        <location>
            <begin position="80"/>
            <end position="198"/>
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            <begin position="1161"/>
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            <begin position="1244"/>
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        </source>
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</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VSC1</accession>
    <name>A0A6E8VSC1_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VSC1"/>
    <dbReference type="EnsemblMetazoa" id="ACON007248-RB">
        <property type="protein sequence ID" value="ACON007248-PB"/>
        <property type="gene ID" value="ACON007248"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_008579"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007248"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030394"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028846">
        <property type="entry name" value="Recoverin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23055">
        <property type="entry name" value="CALCIUM BINDING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23055:SF69">
        <property type="entry name" value="NEURONAL CALCIUM SENSOR 2"/>
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        <source>
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    <sequence length="78" mass="8647" checksum="238A0C3742965181" modified="2020-08-12" version="1">MYDVDGNGVIDIQEMTKIVQAIYDMLGACSSNKPADSAEERAKNIFAKMDENNDGQLTQDEFLKGCLQDEELSKMLAP</sequence>
</entry>
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        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
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            <dbReference type="PubMed" id="30669388"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
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                <consortium name="EnsemblMetazoa"/>
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            <strain evidence="4">Ngousso</strain>
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        <source>
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        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
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                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain>Mali-NIH</strain>
        </source>
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            <authorList>
                <consortium name="EnsemblMetazoa"/>
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        <scope>IDENTIFICATION</scope>
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        </source>
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        <property type="gene ID" value="ACON007250"/>
    </dbReference>
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    <dbReference type="VEuPathDB" id="VectorBase:ACON007250"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030283"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
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        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007250-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="156" mass="18099" checksum="2EA062E4E54E454A" modified="2020-08-12" version="1">MSARRGLRTRVYDANMSMVENNYRAALERLEKSRRAPSLDREVKPRASPFVSRYDFNGADLDEELQVARSRASRVIHEKSTIDQRAEQFRSSSLAPAPVSSALALEGEFDDQVRFRRFLLRRSWVNHNNSVIYVVPLPSDASYFPRCYYDYYSSCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VSD1</accession>
    <name>A0A6E8VSD1_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Adaptor protein enigma</fullName>
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    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
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        <lineage>
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            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
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            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
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    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
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                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
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        <subcellularLocation>
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        </subcellularLocation>
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        <property type="gene ID" value="ACON006901"/>
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    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Zasp-like_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
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        <property type="entry name" value="PDZ AND LIM DOMAIN PROTEIN ZASP"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR24214:SF38">
        <property type="entry name" value="PDZ AND LIM DOMAIN PROTEIN ZASP-RELATED"/>
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        <property type="entry name" value="DUF4749"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="4"/>
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    </dbReference>
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        <property type="match status" value="4"/>
    </dbReference>
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    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
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        <location>
            <begin position="6"/>
            <end position="88"/>
        </location>
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            <begin position="334"/>
            <end position="395"/>
        </location>
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            <begin position="990"/>
            <end position="1049"/>
        </location>
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            <begin position="1050"/>
            <end position="1105"/>
        </location>
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            <begin position="91"/>
            <end position="111"/>
        </location>
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            <begin position="211"/>
            <end position="255"/>
        </location>
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            <end position="301"/>
        </location>
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            <begin position="782"/>
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        </location>
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            <begin position="848"/>
            <end position="914"/>
        </location>
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            <begin position="93"/>
            <end position="102"/>
        </location>
    </feature>
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            <begin position="275"/>
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    </feature>
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            <begin position="500"/>
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            <begin position="901"/>
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            <dbReference type="ARBA" id="ARBA00004496"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022833"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023038"/>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
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    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON006901-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1105" mass="117050" checksum="D581C404F47A2485" modified="2020-08-12" version="1">MANLITVRLQRGDGQAWGFRLQGGKDFSAPLVLQRVNGGSVADQAGLMAGDALIKVNGTEVFNMRHKDAQDVIVAAGNSFELTVSRGGATWRPSVTPTGSLPSPSPSLPGNVTPVTRTSLAATPQEMKPIGSGHNTAAKPFAAVNGNDGQIKSIVNNQYNTPVGMYSDETIAETLSSQAEVLAGGVLGVNFKKNERVYSPANSEVYKLLHEQGDDPEPGNISRTAKARQDARSSAPSLWCRPSTPYKLPTSKLTPTPFQQTASLISRSCSDGTISSQQQQQQPLQQQQQQLQQPLSPHHLSNCGSGTNTVTRHVSAPVDRPVAPAANGLPPGQNICASCERLIVGVFVRIKDKNLHADCFKCATCGTSLKNQGYFNLNDKLYCDIHARLAALNSPPPGTNGMVPVAVTKPGHKTGVQAISAALNAHANLNGRAQSPYGSNGTHTSLGPLSHSPSHSRSSSTTSSSGYCNSSSLSPVQSNKVSPLHSRTSSENELIDRSHSSNSNGSNASELSGNAYPVCPASPPLPPPPPLLTMEECTLQEPGPEVPPAPPQTDASAPLVFGANGLAAPAAPADSEQTDGTVTQNDYGFYYQTMGGRVIRSVLPPGKNSTYKVNQNNITPKPFGAPLPVSPLAQVQPPASYPPVAGSVPTPFSAALVGAPPAQPAPMAAPAPAPMAAAPAPAPMAAPAMAPASVPTPQGSAGPRIEEVDHATLLLDSSVSKLACMSNNADYRKQASSVLKDMLEARLRKTDASVRAKNKENLQHSNGTPAPTIGHRAGGFPLANTNGKAGDEPKPVPPYTNGTAAVPSKQAAFPPNGQHDLSVYSTQITVPVAPLAASELGKVILRPKNSNNHATDMNGSQAPINGRKVSSELTSHARDRRSYVERNNNVAAPHDKKQMQRDSTPTQPQEQDPVANGSALALLKDKNPICNVCDHKIVTGPFITALGRIWCPDHFICHNANCKRPLADIGFVEEKGDLYCEYCFEEFLAPLCSKCNGRVKGDCLNAIGKQFHPECFKCTYCGKQFGNSPFFLEEGDPYCEKDWNDLFTTKCFACGFPVEAGDKWVEALNNNYHSQCFNCTSCKKNLEGQSFFAKGGRPFCKNHAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VSE2</accession>
    <name>A0A6E8VSE2_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="10">C2H2-type domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VSE2"/>
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        <property type="protein sequence ID" value="ACON007377-PA"/>
        <property type="gene ID" value="ACON007377"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000676"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007377"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035055"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="Transcription factor IIIA"/>
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    </dbReference>
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        <property type="entry name" value="zinc finger protein 143 isoform X1"/>
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    </dbReference>
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        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050329">
        <property type="entry name" value="GLI_C2H2-zinc-finger"/>
    </dbReference>
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        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
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        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
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        <property type="entry name" value="METAL REGULATORY TRANSCRIPTION FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19818">
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    </dbReference>
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        <property type="match status" value="4"/>
    </dbReference>
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        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
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        <property type="match status" value="4"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="11"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="41"/>
            <end position="70"/>
        </location>
    </feature>
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            <end position="100"/>
        </location>
    </feature>
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        <location>
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            <end position="127"/>
        </location>
    </feature>
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            <end position="157"/>
        </location>
    </feature>
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        <location>
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            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="171"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="175"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007377-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="666" mass="72536" checksum="38055815DF94719A" modified="2020-08-12" version="1">MGPDNQILARYRCNYGSCNRSYSTVGNLRTHMKTHRGEYKFKCTEEGCTKAFLTSYSQKIHIRVHTKVKPYICGESSCRKAFNTRYRLRAHERLHNGETFNCAVCQKVFTTLSDLKKHSRVHTQERPYKCKEDKCGKAFTASHHLKTHIRTHSGERPFACKINDCNKTFSTPHSLKTHSKTHEKPRKKSSKSGKVKKVRSSPTSEQETDFDIVLIEDENDAAGVQAIDEGTDLHELYDQGATEQQADVLPVAPTAPDVPPEEGFNFVTTNDFHESKALELALAAEEELNPQWIDISMLQTKPLAALDPVTSACVALPTNVPSYVDLSFNVNVADYLPSTEASNVVTSTGGPVLAGDLSTEGPSQVFTIENYFDENLLESANATVQIDRAIQNSEQRAGGVKQEEQEETEKLINELFAGEFLPKMNSSSDAIEPDAGERCDDAADGSSELLNERNSNILLNTPVAVLDGSTKTLKDITADADICRCVNCQCDPMQGCIGGCGPENPCRATAAATSGCNTPSSLLQNMSQSHDRQHPALLPIAAIPTESIVSDNVSLLGLQSQQQQQQQQVQPIKPPPLQSSCCKASATSDDGSSVGGIGSKLTDQLEPLTNMLKSLQNCSCAGPRDGHSKGCCVVICLKTLEALKKVLSTHSSLIQCHNSSANAIMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VSF5</accession>
    <name>A0A6E8VSF5_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_040241148.2">
        <property type="nucleotide sequence ID" value="XM_040385214.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VSF5"/>
    <dbReference type="EnsemblMetazoa" id="ACON007108-RA">
        <property type="protein sequence ID" value="ACON007108-PA"/>
        <property type="gene ID" value="ACON007108"/>
    </dbReference>
    <dbReference type="GeneID" id="120961489"/>
    <dbReference type="CTD" id="10207"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_003475"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007108"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042379"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030054">
        <property type="term" value="C:cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd06667">
        <property type="entry name" value="PDZ2_MUPP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06791">
        <property type="entry name" value="PDZ3_MUPP1-like"/>
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    </dbReference>
    <dbReference type="CDD" id="cd06668">
        <property type="entry name" value="PDZ4_MUPP1-like"/>
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    </dbReference>
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        <property type="entry name" value="PDZ5_MUPP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Multiple PDZ domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000125">
        <property type="entry name" value="PATJ, crumbs cell polarity complex component"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.650">
        <property type="entry name" value="L27 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004172">
        <property type="entry name" value="L27_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036892">
        <property type="entry name" value="L27_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051342">
        <property type="entry name" value="PDZ_scaffold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19964">
        <property type="entry name" value="MULTIPLE PDZ DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19964:SF92">
        <property type="entry name" value="PATJ HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101288">
        <property type="entry name" value="L27 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51022">
        <property type="entry name" value="L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="L27" evidence="6">
        <location>
            <begin position="8"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="5">
        <location>
            <begin position="237"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="5">
        <location>
            <begin position="390"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="5">
        <location>
            <begin position="653"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="5">
        <location>
            <begin position="838"/>
            <end position="916"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007108-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="965" mass="103248" checksum="841B4E886931F0A9" modified="2020-08-12" version="1">MQTAKMHLSSDVSNALKHIEIIKQTVEEMEPNKLQLNVSDDLKLVLDLLQDPVFRNIVQIQDSLAELNHQITQHPSILPGDFDIANGGELVLSVPPGTDLFEADYQDEQRVPSAQISPGSPPGPAGMLAVVTQPKLNLLDQQQLVAGAGPAIVATVPQHHLQQQQQQQPLDAQQLLNASMKLNNEPSLFEDHHHKLLPEPTDQHPLAATGPFTDSPKSVPETIVAGTGPADWSRILDIELVNDGTGLGFGIIGARTTGVTVKTILAGGVADRDGRLKSGDQILQIGDVNLHEMVSEQVASVLRQSGTHVQLVVARPIDPATVGTDEYDHSAIVPTVLLANPLKLKQYLSDSGFGDIYSVYSIANLDSPGLENPFRQESPLPDFPETEVFTVELRKDQNGLGITIAGYVCEKEELSGIFVKSVSPGSAADLSGKIQVNDRIIEVDGQSLHGFSNHQAVDVLKQSGHVVTLCLERYLRGPKYDQLQQAIAANEMKPPTPATPPPPLVPTDLSKYGSNILDILPPQALQRTLGLEQEQAAGEPDDQLAECDVAGLDGGPLHMGGHKKLSRAKDSIDTQEYRDKIKEATIVPDGAVAEDGTLLDATNPVAIARHHHLRSSLKGTATVEETTDSPDPDTAYIVKKWTNILGPEVQIIVANIRKFAASSGLGISLEGTVDVEGGKEVRPHHYIRSILPEGPVGQNGLLRSGDELLEVNGQRLLGMNHLKVVSILKELPQDVCMVCARGDPDLLRFTEEQLISSLEADVAAKRNTQHHHQQLHGSLTPSERLVKAKSDGSLATTNGTGMGGGAGGVGVGDGFSKIKSRSLEPLTGLAMWSSEPQIIELIKGERGLGFSILDYQDPLDPNDTLIVIRSLVPGGVAQLDGRLIPGDRLLFVNDTILENASLDQAVQALKGAPKGVVRIGVAKPLPMQDSSLVAAPATFEERGGVAEGNGSGKMLSLAKPDIIME</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VUG8</accession>
    <name>A0A6E8VUG8_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">ATP-dependent 6-phosphofructokinase</fullName>
            <shortName evidence="15">ATP-PFK</shortName>
            <shortName evidence="15">Phosphofructokinase</shortName>
            <ecNumber evidence="15">2.7.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Phosphohexokinase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 15">Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 15 16">
            <text>beta-D-fructose 6-phosphate + ATP = beta-D-fructose 1,6-bisphosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16109"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="15">Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 15 16">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 15">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade "E" sub-subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade 'E' sub-subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.11" evidence="15"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8VUG8"/>
    <dbReference type="EnsemblMetazoa" id="ACON007642-RA">
        <property type="protein sequence ID" value="ACON007642-PA"/>
        <property type="gene ID" value="ACON007642"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007189"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007642"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039015"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00182"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005945">
        <property type="term" value="C:6-phosphofructokinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003872">
        <property type="term" value="F:6-phosphofructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070095">
        <property type="term" value="F:fructose-6-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048029">
        <property type="term" value="F:monosaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061621">
        <property type="term" value="P:canonical glycolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030388">
        <property type="term" value="P:fructose 1,6-bisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.460:FF:000001">
        <property type="entry name" value="ATP-dependent 6-phosphofructokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.460:FF:000003">
        <property type="entry name" value="ATP-dependent 6-phosphofructokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.450:FF:000043">
        <property type="entry name" value="ATP-dependent 6-phosphofructokinase, platelet type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.450:FF:000064">
        <property type="entry name" value="Phosphofructokinase, platelet b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.450">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.460">
        <property type="entry name" value="Phosphofructokinase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03184">
        <property type="entry name" value="Phosphofructokinase_I_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009161">
        <property type="entry name" value="6-Pfructokinase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022953">
        <property type="entry name" value="ATP_PFK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015912">
        <property type="entry name" value="Phosphofructokinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000023">
        <property type="entry name" value="Phosphofructokinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035966">
        <property type="entry name" value="PKF_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02478">
        <property type="entry name" value="6PF1K_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13697:SF4">
        <property type="entry name" value="ATP-DEPENDENT 6-PHOSPHOFRUCTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13697">
        <property type="entry name" value="PHOSPHOFRUCTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00365">
        <property type="entry name" value="PFK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000533">
        <property type="entry name" value="ATP_PFK_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00476">
        <property type="entry name" value="PHFRCTKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53784">
        <property type="entry name" value="Phosphofructokinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00433">
        <property type="entry name" value="PHOSPHOFRUCTOKINASE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 15" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="11 15 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 15" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13 15 16" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="10 15 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="12 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 15 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 15 16" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphofructokinase" evidence="17">
        <location>
            <begin position="20"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphofructokinase" evidence="17">
        <location>
            <begin position="412"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="region of interest" description="N-terminal catalytic PFK domain 1" evidence="15">
        <location>
            <begin position="1"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="C-terminal regulatory PFK domain 2" evidence="15">
        <location>
            <begin position="411"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="15">
        <location>
            <position position="168"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="90"/>
            <end position="91"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="120"/>
            <end position="123"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <begin position="166"/>
            <end position="168"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <begin position="210"/>
            <end position="212"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <position position="266"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="294"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <begin position="300"/>
            <end position="303"/>
        </location>
        <ligand>
            <name>substrate</name>
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        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <position position="480"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
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        <location>
            <begin position="537"/>
            <end position="541"/>
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        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
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            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
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        <location>
            <position position="575"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
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        <location>
            <begin position="582"/>
            <end position="584"/>
        </location>
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            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
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        <location>
            <position position="638"/>
        </location>
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            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="664"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
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        <location>
            <begin position="670"/>
            <end position="673"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <position position="745"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00002659"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004679"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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            <dbReference type="ARBA" id="ARBA00022842"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00048070"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03184"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR000533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007642-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="789" mass="86683" checksum="A436CAD2FF1B2185" modified="2020-08-12" version="1">MSSAPVQRFIQFGAHKGKGIAVFTSGGDSQGMNAAVRAVVRMGIYVGCKVYFIREGYQGMVDGGDNIVEANWSSVSSIIHRGGTVIGSARCKDFRERAGRLQAAHNLVSRGITNLVVIGGDGSLTGANLFRQEWGSLLDELLASNRITQDQRIKYKSLHIAGMVGSIDNDFCGTDMTIGTDSALHRIIEAIDAIVSTAYSHQRTFIMEVMGRHCGYLAVVAGLCVEADYIFIPEDPPKPDWPERLCKQLTQERSAGQRLNIIIVSEGAIDRDGNPITAEKVKQVVVDRLQQDTRITVLGHVQRGGNPSAFDRVLGCRMGAEAVLALMEADDKTEPCVVSLDGNQAVRVPLMECVERTKAVAKAMEDKNWELAVQLRGRSFARNLETYKMLTRLKPPKSAFDEQGRGVGGYTMAVMHIGAPACGMNAAVRSFVRNCIYRGDTVMGIHDGIEGLVAGHIKKMEWSDVTGWVGQGGAFLGTKRTLPEGKFADIAARLKEFGIQALLIVGGFEAYHAAGQITDQRSKFKEFCIPIVVIPSTISNNMPGTEFSLGADTGLNEITEICDRIRQSAQGTKRRVFVIETMGGYCGYLATLAGLAGGADAAYIYEEKFTIKDLQQDVYHMASKMSDGVQRGLILRNEKASENYNTEFIYRLYSEEGKGLFSTRMNVLGHMQQGGSPTPFDRNMGTKMAAKCVEWLVEQLKANTQPDGSINATAPETACLLGLVRRQYKFSPLKELIGETNFMQRIPKHQWWLKLRPLLRILAKHDSTYEEEGMYITVEEEMSTDSPCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VUH0</accession>
    <name>A0A6E8VUH0_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="19">SSD domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>cholesterol(in) = cholesterol(out)</text>
            <dbReference type="Rhea" id="RHEA:39747"/>
            <dbReference type="ChEBI" id="CHEBI:16113"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the patched family.</text>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON008137-RB">
        <property type="protein sequence ID" value="ACON008137-PB"/>
        <property type="gene ID" value="ACON008137"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006549"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008137"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038612"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015485">
        <property type="term" value="F:cholesterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005319">
        <property type="term" value="F:lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042632">
        <property type="term" value="P:cholesterol homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030301">
        <property type="term" value="P:cholesterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030299">
        <property type="term" value="P:intestinal cholesterol absorption"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1640.10:FF:000008">
        <property type="entry name" value="NPC intracellular cholesterol transporter 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1640.10:FF:000010">
        <property type="entry name" value="NPC intracellular cholesterol transporter 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1640.10">
        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053958">
        <property type="entry name" value="HMGCR/SNAP/NPC1-like_SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004765">
        <property type="entry name" value="NPC1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053956">
        <property type="entry name" value="NPC1_MLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032190">
        <property type="entry name" value="NPC1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000731">
        <property type="entry name" value="SSD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00917">
        <property type="entry name" value="2A060601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45727">
        <property type="entry name" value="NPC INTRACELLULAR CHOLESTEROL TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45727:SF2">
        <property type="entry name" value="NPC INTRACELLULAR CHOLESTEROL TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22314">
        <property type="entry name" value="NPC1_MLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16414">
        <property type="entry name" value="NPC1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12349">
        <property type="entry name" value="Sterol-sensing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82866">
        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50156">
        <property type="entry name" value="SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0153">Cholesterol metabolism</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="10 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="14" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="12" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="334"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="475"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="755"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="784"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="814"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="857"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="891"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="966"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1241"/>
            <end position="1258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1264"/>
            <end position="1284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1296"/>
            <end position="1315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1335"/>
            <end position="1355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1367"/>
            <end position="1390"/>
        </location>
    </feature>
    <feature type="domain" description="SSD" evidence="18">
        <location>
            <begin position="750"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="392"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="412"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008137-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1419" mass="155422" checksum="2F7327DCC476F125" modified="2020-08-12" version="1">MKMISGNTRRAACPSEAAAAAGTGKATSCNRRQSSSTAACWFFVPLLVLGSLLIDQAQCQTIGGDESGGNQCVWYGICNVDSLGRAQYCPYNGTAKAIDTEANDLLKVWCKHLLVEDATGSINTCCDAAQVKVLNKNVALAANFLSRCPSCMANLVRHMCDFTCSPQQSSFMKVVSTEEVDQQEGESEKADATTAAPSPKEYITKIDIHITQQYLNGTFESCNQVSVPSTGQLALDLMCGDWGASRCSAKKWFFYMGDAENNLYVPFQIDYVAHSSPNETIDGYLPWNPRIVPCSEKLDANTPACSCVDCEASCPKPPAPPGPPQPFVIYGMDGYAVVMFVVFVVCSGLFLIGACFCHSSTGTGGGSGSGAKLLVNSGDALPSDLRSSVGRRLAGGLSSNSGDLGTDREDSPLQSKRSSATWDGEQELRPHPSGVGAADDDDDESGYFERLGAKTETALEHFFTAWGTTCAKHPWLVLLGGLLFIVAMGMGINFLRVTTNPVELWASPYSRSRVEREYFDSNFEPFYRIEQIIIKAENISNVMHNTSNGVIQFGPVFNRQFLLDVFELQEQIKKIQAVAVDGSGNNETIMLKDICFAPLSTGAGPTGTQECVVQSLWGYFSDDMDNFNDEEEDAQGFVINYLDKLIQCFGNYYNPACLAPYGGPIDPAIALGGIPQPKSADEKASYAEASAVILTFLVRNYHDKNKLQSALAWETEYVRFMQNWTRENMSIAFTSERSIEDELARESQSDVSTILVSYIIMFAYIAISLGHVNQWNRALIDSKVTLGLGGVAIVLASVVASVGIFGYIGVPATLIIVEVIPFLVLAVGVDNIFILVQTHQRDTKKPTETHAEHIGRILGRVGPSILLTSVSESCCFFLGGLSDMPAVRAFALYAGMALLIDFILQITCFVSLLALDTIRQTDNRLDVLCFLRGSKKDMPGNIGEGLLYKFFKSIYVPFVMRKPVRVAVMIVFFGWLCSSIAVAPHIDIGLDQELSMPGDSFVLKYFRYLQQYLSIGPPVYFVVKNGLNYSTMNDQNLICGGQYCNLDSLSTQLYIASKQPQSTYLARPASSWLDDYIDWSAAPGCCKQWNNGSFCPHQKSACGACNISMTAQKRPVESSFRQYVSFFLEDNPDEACAKAGHAAYGSGVKYRPDALTPLYNDVGASYFMAYHTILKSSSDYYEALRSARKISANITSTIHANLRLQGRSEADIQQIEVFPYSVFYVFYEQYLTMWPDTLKSMGISVLAIFIVTFLLMGFDIHSSLVVVITITMIVINIGGLMYHWSISLNAVSLVNLVMAVGISVEFCSHLVHSFAMSVEETREKRAADALTKMGSSVFSGITLTKFGGILVLGFAHSQIFQVFYFRMYLGIVLYGAAHGLVFLPVLLSYIGAPINKVKLANHRRQAMQETQETSLSTTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VUJ7</accession>
    <name>A0A6E8VUJ7_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">CRAL-TRIO domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VUJ7"/>
    <dbReference type="EnsemblMetazoa" id="ACON008165-RC">
        <property type="protein sequence ID" value="ACON008165-PC"/>
        <property type="gene ID" value="ACON008165"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON008165-RD">
        <property type="protein sequence ID" value="ACON008165-PD"/>
        <property type="gene ID" value="ACON008165"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON008165-RE">
        <property type="protein sequence ID" value="ACON008165-PE"/>
        <property type="gene ID" value="ACON008165"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON008165-RF">
        <property type="protein sequence ID" value="ACON008165-PF"/>
        <property type="gene ID" value="ACON008165"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007724"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008165"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042427"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902936">
        <property type="term" value="F:phosphatidylinositol bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1200">
        <property type="entry name" value="Alpha-tocopherol transfer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.20">
        <property type="entry name" value="N-terminal domain of phosphatidylinositol transfer protein sec14p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011074">
        <property type="entry name" value="CRAL/TRIO_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10174">
        <property type="entry name" value="ALPHA-TOCOPHEROL TRANSFER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10174:SF220">
        <property type="entry name" value="LD41874P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00180">
        <property type="entry name" value="CRETINALDHBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM01100">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="1">
        <location>
            <begin position="111"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008165-PF"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="313" mass="36769" checksum="4A0B02480708E6A0" modified="2020-08-12" version="1">MLSDVEELPSVQLGDYVLRFELEDLTPFGKEVAKNELRETPEIRAAAIEELRTLLKDVEDLVVPLDNDDWMIRFLRPCKFYAKSACELIQRYYQFKVKHFDMYNELMPSKEANIFKQNILAVFPNRDQLGRRVLLLELGKTWKHKEVSLDEVFKGCVLFLEAAMLEPETQVHGAVVIFDMDGLTLQQAWQFTPPFAKRIVDWLQDAVPLRIKGIHIINQPKIFNMVFALFKPILREKLRNRIIFHGTDRESLYKHISKECLPAVYGGTVDAPRVQGEQWYELLLKCDNEYKEINNYGYIKQLEEQRSKKNKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VWK5</accession>
    <name>A0A6E8VWK5_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Protein TsetseEP domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VWK5"/>
    <dbReference type="EnsemblMetazoa" id="ACON008306-RA">
        <property type="protein sequence ID" value="ACON008306-PA"/>
        <property type="gene ID" value="ACON008306"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012408"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008306"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039599"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008306-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="53" mass="6165" checksum="8B7289BBC7181CCD" modified="2020-08-12" version="1">MTRLQQMVEDFTACRQEATANDPQHDCSDSIQRAKVDLQQQLVNYSYCTKNIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VWL3</accession>
    <name>A0A6E8VWL3_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Fuseless</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_040231690.1">
        <property type="nucleotide sequence ID" value="XM_040375756.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VWL3"/>
    <dbReference type="EnsemblMetazoa" id="ACON008310-RB">
        <property type="protein sequence ID" value="ACON008310-PB"/>
        <property type="gene ID" value="ACON008310"/>
    </dbReference>
    <dbReference type="GeneID" id="120955171"/>
    <dbReference type="CTD" id="33765"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005253"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008310"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031613"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042734">
        <property type="term" value="C:presynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070073">
        <property type="term" value="P:clustering of voltage-gated calcium channels"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007274">
        <property type="term" value="P:neuromuscular synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007270">
        <property type="term" value="P:neuron-neuron synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032751">
        <property type="entry name" value="Fuseless"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35270">
        <property type="entry name" value="FUSELESS, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35270:SF2">
        <property type="entry name" value="FUSELESS, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15993">
        <property type="entry name" value="Fuseless"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="29"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="68"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="107"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="134"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="188"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="227"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="258"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="301"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="373"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="385"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="396"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008310-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="442" mass="50144" checksum="0280DEACD47F17A3" modified="2020-08-12" version="1">MRGSFLRLDQLTGSARKTLANDEPKVPDIVNSIYAALVIGPLVICFWRGTWNLMDMLLFPEDEVLRMITLQVFGLGGHLFFMLLQTTLERWLDVEVRPVSFYLLSRVYTYIYGAMCVATWRGGWILIDRYSPPSLVFFMEASLVALLLLGSIQSFCNVAGAPFVNDSPDGYFDVPTYFKSKGSKDPGLYVLDCMFSVLVIGSLVVFVWRGLWVLLDLKLFPEDHGTSAWASIIIGYGVTGVTFSLQPLMRWTCDRLTGFWRVVVADLFLFFSFIGTINVWRGIWQALDTYFLPDDPLLSNWITHGAALLLLILLNCSNSVLVRGVYIDAEEPAGQCVVFPVYYIRLFFQKERKKKQRRLFDALERADLNSTGGVLQDKKHHHSLQTAAQQPAQQKEQQKEAHQHHIAADQNHQVIRNLEAVPLTIIDDPKSNGDHSSQPTRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VWL8</accession>
    <name>A0A6E8VWL8_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">HTH La-type RNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_040235837.2">
        <property type="nucleotide sequence ID" value="XM_040379903.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VWL8"/>
    <dbReference type="EnsemblMetazoa" id="ACOM040599-RA">
        <property type="protein sequence ID" value="ACOM040599-PA.1"/>
        <property type="gene ID" value="ACOM040599"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON008952-RA">
        <property type="protein sequence ID" value="ACON008952-PA"/>
        <property type="gene ID" value="ACON008952"/>
    </dbReference>
    <dbReference type="GeneID" id="120957614"/>
    <dbReference type="KEGG" id="acoz:120957614"/>
    <dbReference type="CTD" id="35305"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004580"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008952"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_036724"/>
    <dbReference type="Proteomes" id="UP000075882">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045727">
        <property type="term" value="P:positive regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08028">
        <property type="entry name" value="LARP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12291">
        <property type="entry name" value="RRM1_La"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045180">
        <property type="entry name" value="La_dom_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006630">
        <property type="entry name" value="La_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014886">
        <property type="entry name" value="La_xRRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002344">
        <property type="entry name" value="Lupus_La"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22792:SF166">
        <property type="entry name" value="LUPUS LA PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22792">
        <property type="entry name" value="LUPUS LA PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05383">
        <property type="entry name" value="La"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08777">
        <property type="entry name" value="RRM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00302">
        <property type="entry name" value="LUPUSLA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00715">
        <property type="entry name" value="LA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50961">
        <property type="entry name" value="HTH_LA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51939">
        <property type="entry name" value="XRRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="HTH La-type RNA-binding" evidence="8">
        <location>
            <begin position="24"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="128"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="XRRM" evidence="9">
        <location>
            <begin position="244"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="338"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="210"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="343"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="360"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="388"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008952-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="412" mass="46862" checksum="8F3C27AC4DCCB67F" modified="2020-08-12" version="1">MTETDVQAPAVEETKPEGNEENATATVSTTEAAIIRQLEYYFGDANLAKDKFMKDQIAKDEGWVPLDVLLTFKRLKSLSEDKKVIVDAIAKSDEGLVEVSEDREKLRRHPERPLPEMNEERRQEIYARTVYVKGFAPQNGTTMSELVEFFEPFTKVTNIVMRKYHDKPTKKYLFKGSVFVTFAEKEQCEKFLAQGPLKYKGKELICKMQNDYYEAKKVEIKERAEKIEERKRKKEEGNNALLAQLPTGACVFMEGLGKETTRESIKEAIRALDETLEFAYIDYQKGDASGYVRFNKQDAGKQLLEKLTDKKLKIDDAEVTAELLVDEKETEYLKKIAEEQAKRKVNAKNKGRGNRGNNNRNHHSGGNNKRKRDRNAEQQEDGDGGEPPVKKDATGKEATKPTAESEAKAAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VWM1</accession>
    <name>A0A6E8VWM1_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">CD109 antigen</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">TEP1-F</fullName>
        </alternativeName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="24">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Binds covalently through a thioester bond to the pathogen surface resulting in pathogen clearance.</text>
    </comment>
    <comment type="function">
        <text evidence="14">Modulates negatively TGFB1 signaling in keratinocytes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">Heterodimer of a TEP1-N chain and an TEP1-C chain non-covalently linked. Forms a complex composed of TEP1-N and TEP1-C heterodimer, LRIM1 and APL1C; the interaction stabilizes TEP1-N and TEP1-C heterodimer, prevents its binding to tissues while circulating in the hemolymph and protects the thioester bond from hydrolysis. Mature TEP1 and to a lesser extent full-length TEP1 interact with SPCLIP1; the interaction is induced by microbial infection.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Heterodimer; disulfide-linked. Interacts with TGFB1 and TGFBR1. Forms a heteromeric complex with TGFBR1, TGFBR2 and TGFBR3 in a ligand-independent manner.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.</text>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON008364-RD">
        <property type="protein sequence ID" value="ACON008364-PD"/>
        <property type="gene ID" value="ACON008364"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000438"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008364"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038887"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004866">
        <property type="term" value="F:endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02897">
        <property type="entry name" value="A2M_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.20:FF:000001">
        <property type="entry name" value="CD109 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1930:FF:000001">
        <property type="entry name" value="CD109 isoform 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000155">
        <property type="entry name" value="complement C3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.130.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1930">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.690">
        <property type="entry name" value="Alpha-macroglobulin, receptor-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009048">
        <property type="entry name" value="A-macroglobulin_rcpt-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036595">
        <property type="entry name" value="A-macroglobulin_rcpt-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050473">
        <property type="entry name" value="A2M/Complement_sys"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011625">
        <property type="entry name" value="A2M_N_BRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041813">
        <property type="entry name" value="A2M_TED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047565">
        <property type="entry name" value="Alpha-macroglob_thiol-ester_cl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011626">
        <property type="entry name" value="Alpha-macroglobulin_TED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001599">
        <property type="entry name" value="Macroglobln_a2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019742">
        <property type="entry name" value="MacrogloblnA2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002890">
        <property type="entry name" value="MG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041555">
        <property type="entry name" value="MG3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040839">
        <property type="entry name" value="MG4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11412:SF136">
        <property type="entry name" value="CD109 ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11412">
        <property type="entry name" value="MACROGLOBULIN / COMPLEMENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00207">
        <property type="entry name" value="A2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07703">
        <property type="entry name" value="A2M_BRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07677">
        <property type="entry name" value="A2M_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01835">
        <property type="entry name" value="MG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17791">
        <property type="entry name" value="MG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17789">
        <property type="entry name" value="MG4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07678">
        <property type="entry name" value="TED_complement"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01360">
        <property type="entry name" value="A2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01359">
        <property type="entry name" value="A2M_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01361">
        <property type="entry name" value="A2M_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01419">
        <property type="entry name" value="Thiol-ester_cl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49410">
        <property type="entry name" value="Alpha-macroglobulin receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00477">
        <property type="entry name" value="ALPHA_2_MACROGLOBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8" id="KW-0391">Immunity</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0882">Thioester bond</keyword>
    <feature type="domain" description="Alpha-2-macroglobulin bait region" evidence="21">
        <location>
            <begin position="457"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-2-macroglobulin" evidence="22">
        <location>
            <begin position="706"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-macroglobulin receptor-binding" evidence="23">
        <location>
            <begin position="1325"/>
            <end position="1414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1436"/>
            <end position="1458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="1444"/>
            <end position="1458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00056820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00057615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00063008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00063781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00069665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00078071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="SMART" id="SM01359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="SMART" id="SM01360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="SMART" id="SM01361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008364-PD"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1482" mass="164455" checksum="85289A7802F4CE12" modified="2020-08-12" version="1">MRRSTMFASKKDPAIRILSTSSSSLLAVCLVLSVVLVPAVQCEGHYSIVGAKLLRPNSEYHVAVTNQDVSEPIRFSLAITDASSVIAKQEITLNTGETRLVPFAIGDIPESSYKLVAEGLSGLTFKNETDLEYQQKSFSVFVQTDKSIYKPGDTVRFRVLVLDPNTKPLQKADNISVHINDAKANRIKQWKEGKLVKGVFESELTLSTAPVLGAWTINVEVLGSKHNKVFEVDEYVLPKFEVTVESPGITTFKDGKVKAIIRSKYTYGKPVKGEATVSVSPEFQFNYVQPFAKDVITRKVIPIDGKGSVEFDLREDIHLEGDYSRNIVIEAVVEEELTGRKQNASAKVMIYDRRYKMELVKSDDNFKPGLPYTAWLKVSYQDGAPVQDQTNPVEVKQSSFESTTSVQNYTLDQNGMAKLEINTEVNSSYINVVGVYLGQEFYLHGISKAESDVDSYIRAQVLTEMPLVGKDVLVEVTSTSPMKYFTYQLLGRGDVLLSNTIAVPESKTQSFKFPATFAMVPRAKLVVYYIASNGDMVSDSKVITFDSELQNFMKVSLSKEQSKPGQDVEISISTNPDSYVGLLGVDQSVLLLKSGNDITKQQVFSELEKYEERSYGFYRRKKRFAWNPHAEHRDFSTVGAFVMSNANDPPQRFRYVSLMMPMAAFGTSISLRSGPVTRPFSGLAAPGRAPVRASAPAPVRKEFPETWLWQTIAPASFSGEKTLQKKVPDTITSWIITGFSVNPIYGLGLTQQPRKLNVFLPFFVSTNLPYSVKRGEVVAIPIVVFNYMEDDQTAEVVLHNDEQEFEFADVENEVVESNKVELFRQKRLDIASNTGKSVSFMVKPKKLGHITIKVTAKTKIAGDAVERQLLVEPEGLPQFINKAAFIDLRAAPELTKTFEVEIPKNAVPDSTRIEVAVIGDVMGSTIQNLDSLIRMPYGCGEQNMLNFVPNIVVLDYLKATNKLTANIEAKAKKFMEAGYQRELGYKHRDGSFSAFGENDKSGSTWLTAFVARSFKQAANHITIDEGVIDKSLEWLSDHQAPNGSFPEVGVVSHKDMQGGSGSGVALTAYTLIAFLENINLVDKYKNTINKAIDYVYRNTESLDDTYALALAAYALQLADHSSKGLILSKLDTKATTDSDSKWWHKPIPETEQKNPWYSRPNSVNVEMSAYGMLAFLEAGLDTDALPIMKWLIGQRNDKGGFQSTQDTVVGLQALAKLAAKITSPNNDVTLTAKINENQEKRMTVNAENGMILQKFELPSAARNIEIQATGSGFAVVQLSYKYNMNVTGEWPRFVLDPQVNANTNPDYLHLSVCASFVPSAGQNVSNMAVMEVGFPSGFTADSDTLPSLENMPFIKKVETKDGDTTVVLYFDSLDQRELCPTISAFRTHKVAKQKPAPVVIYDYYDNSRIARQFYDGPKASLCDICENEDCGEACSIRSQKQRSSDSPSRQPTVEGTMQSGSQTVSVSFFTFLLATLLVRMFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VWM5</accession>
    <name>A0A6E8VWM5_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Tyrosine-protein kinase</fullName>
            <ecNumber evidence="13">2.7.10.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 13">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the protein kinase superfamily. Tyr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.2" evidence="13"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8VWM5"/>
    <dbReference type="EnsemblMetazoa" id="ACON008317-RA">
        <property type="protein sequence ID" value="ACON008317-PA"/>
        <property type="gene ID" value="ACON008317"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002310"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008317"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035633"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004715">
        <property type="term" value="F:non-membrane spanning protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048468">
        <property type="term" value="P:cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002009">
        <property type="term" value="P:morphogenesis of an epithelium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007424">
        <property type="term" value="P:open tracheal system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007435">
        <property type="term" value="P:salivary gland morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd09934">
        <property type="entry name" value="SH2_Tec_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11768">
        <property type="entry name" value="SH3_Tec_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000052">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000190">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000053">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000045">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050198">
        <property type="entry name" value="Non-receptor_tyrosine_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24418">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="1 11" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="3 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 13" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="SH3" evidence="15">
        <location>
            <begin position="21"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="14">
        <location>
            <begin position="89"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="205"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00051245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU362096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008317-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="465" mass="52974" checksum="71B0A4E13739F1FA" modified="2020-08-12" version="1">MPGGPTVTPGTPNTKAKDVSPRVKIVVALYPFKAIESGDLSLEKNAEYEVIDDSQEHWWKVKDQHGTIGYIPSNYVKEKELLGLQKYDWYVGDMSRQRAESLLKQNDKEGCFVVRKSSTKGLYTLSLHTKVPQSHVKHYHIKQNARSEFYLSEKHCCETIPDLINYHRHNSGGLACRLKTSPCDRPVPPTAGLSHDKWEIHPSELMILEELGSGQFGVVRRAKWRGSIDTAVKMMKVGTMSEDDFIEEAKVMTKLQHPNLVQLYGVCSKHRPIYIITEYMKHGSLLNYLRRHEQSLIGNMGLLLDMCIQVCKGMSYLERHNYIHRDLAARNCLVGSENTVKVADFGLARYVLDDQYTSSGGTKFPIKWAPPEVLNYTRFSSKSDVWAYGVLMWEVFTCGKMPYGRLKNTEVVERVQRGIILEKPKACAKEIYDVMKKCWSHSPEDRPGFRILKDQLAAVAQGLTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VWM6</accession>
    <name>A0A6E8VWM6_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">CD109 antigen</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">TEP1-F</fullName>
        </alternativeName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="24">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Binds covalently through a thioester bond to the pathogen surface resulting in pathogen clearance.</text>
    </comment>
    <comment type="function">
        <text evidence="14">Modulates negatively TGFB1 signaling in keratinocytes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">Heterodimer of a TEP1-N chain and an TEP1-C chain non-covalently linked. Forms a complex composed of TEP1-N and TEP1-C heterodimer, LRIM1 and APL1C; the interaction stabilizes TEP1-N and TEP1-C heterodimer, prevents its binding to tissues while circulating in the hemolymph and protects the thioester bond from hydrolysis. Mature TEP1 and to a lesser extent full-length TEP1 interact with SPCLIP1; the interaction is induced by microbial infection.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Heterodimer; disulfide-linked. Interacts with TGFB1 and TGFBR1. Forms a heteromeric complex with TGFBR1, TGFBR2 and TGFBR3 in a ligand-independent manner.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.</text>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON008364-RI">
        <property type="protein sequence ID" value="ACON008364-PI"/>
        <property type="gene ID" value="ACON008364"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000438"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008364"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038887"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004866">
        <property type="term" value="F:endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02897">
        <property type="entry name" value="A2M_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.20:FF:000001">
        <property type="entry name" value="CD109 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1930:FF:000001">
        <property type="entry name" value="CD109 isoform 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000155">
        <property type="entry name" value="complement C3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.130.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1930">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.690">
        <property type="entry name" value="Alpha-macroglobulin, receptor-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009048">
        <property type="entry name" value="A-macroglobulin_rcpt-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036595">
        <property type="entry name" value="A-macroglobulin_rcpt-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050473">
        <property type="entry name" value="A2M/Complement_sys"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011625">
        <property type="entry name" value="A2M_N_BRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041813">
        <property type="entry name" value="A2M_TED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047565">
        <property type="entry name" value="Alpha-macroglob_thiol-ester_cl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011626">
        <property type="entry name" value="Alpha-macroglobulin_TED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001599">
        <property type="entry name" value="Macroglobln_a2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019742">
        <property type="entry name" value="MacrogloblnA2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002890">
        <property type="entry name" value="MG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041555">
        <property type="entry name" value="MG3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040839">
        <property type="entry name" value="MG4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11412:SF136">
        <property type="entry name" value="CD109 ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11412">
        <property type="entry name" value="MACROGLOBULIN / COMPLEMENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00207">
        <property type="entry name" value="A2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07703">
        <property type="entry name" value="A2M_BRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07677">
        <property type="entry name" value="A2M_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01835">
        <property type="entry name" value="MG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17791">
        <property type="entry name" value="MG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17789">
        <property type="entry name" value="MG4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07678">
        <property type="entry name" value="TED_complement"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01360">
        <property type="entry name" value="A2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01359">
        <property type="entry name" value="A2M_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01361">
        <property type="entry name" value="A2M_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01419">
        <property type="entry name" value="Thiol-ester_cl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49410">
        <property type="entry name" value="Alpha-macroglobulin receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00477">
        <property type="entry name" value="ALPHA_2_MACROGLOBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8" id="KW-0391">Immunity</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0882">Thioester bond</keyword>
    <feature type="domain" description="Alpha-2-macroglobulin bait region" evidence="21">
        <location>
            <begin position="457"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-2-macroglobulin" evidence="22">
        <location>
            <begin position="704"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-macroglobulin receptor-binding" evidence="23">
        <location>
            <begin position="1321"/>
            <end position="1410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1432"/>
            <end position="1454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="1440"/>
            <end position="1454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00056820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00057615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00063008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00063781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00069665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00078071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="SMART" id="SM01359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="SMART" id="SM01360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="SMART" id="SM01361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008364-PI"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1478" mass="164114" checksum="7E738F360293580D" modified="2020-08-12" version="1">MRRSTMFASKKDPAIRILSTSSSSLLAVCLVLSVVLVPAVQCEGHYSIVGAKLLRPNSEYHVAVTNQDVSEPIRFSLAITDASSVIAKQEITLNTGETRLVPFAIGDIPESSYKLVAEGLSGLTFKNETDLEYQQKSFSVFVQTDKSIYKPGDTVRFRVLVLDPNTKPLQKADNISVHINDAKANRIKQWKEGKLVKGVFESELTLSTAPVLGAWTINVEVLGSKHNKVFEVDEYVLPKFEVTVESPGITTFKDGKVKAIIRSKYTYGKPVKGEATVSVSPEFQFNYVQPFAKDVITRKVIPIDGKGSVEFDLREDIHLEGDYSRNIVIEAVVEEELTGRKQNASAKVMIYDRRYKMELVKSDDNFKPGLPYTAWLKVSYQDGAPVQDQTNPVEVKQSSFESTTSVQNYTLDQNGMAKLEINTEVNSSYINVVGVYLGQEFYLHGISKAESDVDSYIRAQVLTEMPLVGKDVLVEVTSTSPMKYFTYQLLGRGDVLLSNTIAVPESKTQSFKFPATFAMVPRAKLVVYYIASNGDMVSDSKVITFDSELQNFMKVSLSKEQSKPGQDVEISISTNPDSYVGLLGVDQSVLLLKSGNDITKQQVFSELEKYEERSYGFYRRKKRFAWNPHAEHRDFSTVGAFVMSNANDPPRSAVLRCCDDTDDLLDIRVAEEDRIGSALEYAPSTLSSAPISEGPVVRKSFPESWIWHTIASFSGEKTLQKKVPDTITSWIITGFSVNPIYGLGLTQQPRKLNVFLPFFVSTNLPYSVKRGEVVAIPIVVFNYMEDDQTAEVVLHNDEQEFEFADVENEVVESNKVELFRQKRLDIASNTGKSVSFMVKPKKLGHITIKVTAKTKIAGDAVERQLLVEPEGLPQFINKAAFIDLRAAPELTKTFEVEIPKNAVPDSTRIEVAVIGDVMGSTIQNLDSLIRMPYGCGEQNMLNFVPNIVVLDYLKATNKLTANIEAKAKKFMEAGYQRELGYKHRDGSFSAFGENDKSGSTWLTAFVARSFKQAANHITIDEGVIDKSLEWLSDHQAPNGSFPEVGVVSHKDMQGGSGSGVALTAYTLIAFLENINLVDKYKNTINKAIDYVYRNTESLDDTYALALAAYALQLADHSSKGLILSKLDTKATTDSDSKWWHKPIPETEQKNPWYSRPNSVNVEMSAYGMLAFLEAGLDTDALPIMKWLIGQRNDKGGFQSTQDTVVGLQALAKLAAKITSPNNDVTLTAKINENQEKRMTVNAENGMILQKFELPSAARNIEIQATGSGFAVVQLSYKYNMNVTGEWPRFVLDPQVNANTNPDYLHLSVCASFVPSAGQNVSNMAVMEVGFPSGFTADSDTLPSLENMPFIKKVETKDGDTTVVLYFDSLDQRELCPTISAFRTHKVAKQKPAPVVIYDYYDNSRIARQFYDGPKASLCDICENEDCGEACSIRSQKQRSSDSPSRQPTVEGTMQSGSQTVSVSFFTFLLATLLVRMFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VYN3</accession>
    <name>A0A6E8VYN3_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VYN3"/>
    <dbReference type="EnsemblMetazoa" id="ACON009773-RB">
        <property type="protein sequence ID" value="ACON009773-PB"/>
        <property type="gene ID" value="ACON009773"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012900"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON009773"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042798"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009773-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="122" mass="13833" checksum="A2C85DA560C9A3C0" modified="2020-08-12" version="1">MSGLMMPVIVYASDEPLQTAKVCQYMQCTVSENDGAQVRLRFNHTMWKQYSGSLNQEFDFTSWSWSELDSVAEANPTLEVTFNGLEVPHVDIVILPAQQTPTAGFIEQLDQRNQRLQIIHLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VYQ2</accession>
    <name>A0A6E8VYQ2_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Lipase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VYQ2"/>
    <dbReference type="EnsemblMetazoa" id="ACON009104-RC">
        <property type="protein sequence ID" value="ACON009104-PC"/>
        <property type="gene ID" value="ACON009104"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000234"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON009104"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041429"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016298">
        <property type="term" value="F:lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017171">
        <property type="term" value="F:serine hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00707">
        <property type="entry name" value="Pancreat_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000122">
        <property type="entry name" value="Vitellogenin-3-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013818">
        <property type="entry name" value="Lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033906">
        <property type="entry name" value="Lipase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000734">
        <property type="entry name" value="TAG_lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610:SF37">
        <property type="entry name" value="GH01208P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610">
        <property type="entry name" value="LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00151">
        <property type="entry name" value="Lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00821">
        <property type="entry name" value="TAGLIPASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Lipase" evidence="5">
        <location>
            <begin position="32"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009104-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="327" mass="36265" checksum="2DCF42B9C1C1907F" modified="2020-08-12" version="1">MNKVLVVVSGLVIAVGVWVGMKPVRAHLDTAKFVLFYGPKFEDHETYSLEKAEDILQHSRFDKSKKTVMYFHGYIESMEVESVHVIADAYLKRGDHNIIILDWAQLADGNYLLEAVPNCKKLGSYLGSVVLRMINAGLNVDKLHLVGHSLGGQLAGYVGRTVIAQSEKRVKLNRISALDPAFPPFYPGIFATALSSKDAEFVDVIHTDAWLYGAPVSTGTADFWPNNGKTLQPGCPKRNYKLLTDNDLCSHRRSWWFWAESVAESNVASFHSVKCKSWDDFKDGKVDRAAPIVHMGIDCSSDAKGDYYLQTNGAPPYSKGVSGSKFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VYQ5</accession>
    <name>A0A6E8VYQ5_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Gustatory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Gustatory receptor which mediates acceptance or avoidance behavior, depending on its substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the insect chemoreceptor superfamily. Gustatory receptor (GR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VYQ5"/>
    <dbReference type="EnsemblMetazoa" id="ACON009805-RD">
        <property type="protein sequence ID" value="ACON009805-PD"/>
        <property type="gene ID" value="ACON009805"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACON009805"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034541"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007635">
        <property type="term" value="P:chemosensory behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008049">
        <property type="term" value="P:male courtship behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050909">
        <property type="term" value="P:sensory perception of taste"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013604">
        <property type="entry name" value="7TM_chemorcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21143:SF134">
        <property type="entry name" value="GUSTATORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21143">
        <property type="entry name" value="INVERTEBRATE GUSTATORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08395">
        <property type="entry name" value="7tm_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="70"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="125"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="159"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="225"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="345"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009805-PD"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="388" mass="45476" checksum="5487F202BA75510D" modified="2020-08-12" version="1">MSMYIRQNHFTLRIVTAIYFLPVSYNQTTQRFVQQRTNLVSFGVGILLSFTFFYHDIFIQNVFFANLPPFAFAIVLLELLLYTIVPFCVVLNNLFYRKRWTDLLNVLFADDSVLDTTNVTMSGAYFLYISVLSILLVTLELCNLFALETLSHKMLTTTFVLRYTGMIHFLYLFHMCVSMVGLRMEQLKMLFHHKQTEDEFEYFLGLFMAKFERYVMLIEQINRCFSLPIVTMLALGMVELAYLVFECFYTYDAGSPDTEMYNGFTDRAFSQFWQSMYCHFLVLTVSSCERTRKMVEETALCTRHFDDYRLQNTRAAKQIQKFLLKNLHQKKKFSACGFFDIDNTVIYMVFSSIVTYLVILIQFKQLETDLTQAGDGYNVTSNVSTVQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VYQ9</accession>
    <name>A0A6E8VYQ9_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Gustatory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Gustatory receptor which mediates acceptance or avoidance behavior, depending on its substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the insect chemoreceptor superfamily. Gustatory receptor (GR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VYQ9"/>
    <dbReference type="EnsemblMetazoa" id="ACON009805-RI">
        <property type="protein sequence ID" value="ACON009805-PI"/>
        <property type="gene ID" value="ACON009805"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACON009805"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007635">
        <property type="term" value="P:chemosensory behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008049">
        <property type="term" value="P:male courtship behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050909">
        <property type="term" value="P:sensory perception of taste"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013604">
        <property type="entry name" value="7TM_chemorcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21143:SF134">
        <property type="entry name" value="GUSTATORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21143">
        <property type="entry name" value="INVERTEBRATE GUSTATORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08395">
        <property type="entry name" value="7tm_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="35"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="72"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="127"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="173"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="353"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009805-PI"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="396" mass="46023" checksum="610838967C20CBCF" modified="2020-08-12" version="1">MECLQIMFQSLSALGMTLVRYDVRHRSYSKPTSFQFSVQLLYSLCCITFTSFRVWVQFSVHSQIEHEMLENISFIVSTASFCIIWMVVPLYAYARCSRLVHALNSLQANDAELGHVIMLSYRRVKCFACFIQLDGVLCCLLLSTGYTIAFWYQGQPWYIFLNIGIYIYEDLCLSWLFGIYSAIMLFCVAQLSCAAKLLQSDYYHSDTLETRLRLFCRTYDRVCTRVAQPFYEYCGPVVATFCPLLIFEGSLKLFHAYDVFKSSTYRTVWTEQLLSASEWLWLSFDVKKLFVVLYVSEVLKQKVEETALCTRHFDDYRLQNTRAAKQIQKFLLKNLHQKKKFSACGFFDIDNTVIYMVFSSIVTYLVILIQFKQLETDLTQAGDGYNVTSNVSTVQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VZ06</accession>
    <name>A0A6E8VZ06_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the MICU1 family. MICU1 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VZ06"/>
    <dbReference type="EnsemblMetazoa" id="ACON009117-RB">
        <property type="protein sequence ID" value="ACON009117-PB"/>
        <property type="gene ID" value="ACON009117"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012799"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON009117"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_043047"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990246">
        <property type="term" value="C:uniplex complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036444">
        <property type="term" value="P:calcium import into the mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051560">
        <property type="term" value="P:mitochondrial calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15900">
        <property type="entry name" value="EFh_MICU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039800">
        <property type="entry name" value="MICU1/2/3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12294:SF1">
        <property type="entry name" value="CALCIUM UPTAKE PROTEIN 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12294">
        <property type="entry name" value="EF HAND DOMAIN FAMILY A1,A2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13202">
        <property type="entry name" value="EF-hand_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13833">
        <property type="entry name" value="EF-hand_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="EF-hand" evidence="15">
        <location>
            <begin position="276"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="15">
        <location>
            <begin position="467"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="141"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="143"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009117-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="533" mass="60579" checksum="8FF2F73B52452926" modified="2020-08-12" version="1">MALLRQISGVLNRGFGGLSKGPVHPVLLSGRYGIHPAHWRLLSTLSGGPSQAARLGAAGEHTLRVVPGTAWQDSNRQRQRGYKNFGHQHEKEPLYTKLFYVFLCSVMVGSCLDWKKLGAAINWPKVDADAGVEMGKQNGTQELEQEEEDADAESDVDAQGRKKSRKEKIGFRDRKIIEYENRIRQYSTPDKVFRYFATIHDGVEVFMTPIDFLTSMTPGMKQPEGLGLDQYKRYDAKSVSTRLDLHLDSDSIFYKLGAYGLISFSDYIFLLTVLSTSRRHFEIAFRMFDLNGDGDVDSEEFEKVANLIRQQTSIGNRHRDHANTGNTFKGVNSALTTYFFGPKNDEKLTIEKFLDFQQQLQREILTLEFLRKNPDENGNISEADFAELLLAYAGYPQKKKVKKIKRVKKRFRDHGSGISKEDYLNFFHFLNNINDVDTALTFYHIAGASIDQETLKHVARTVALVELSSHVIDVVFTIFDENMDGQLSNREFVAVMKNRLLRGLEKPKDTGFVKLMHSILKCAKDTKPVLLDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VZ07</accession>
    <name>A0A6E8VZ07_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Importin N-terminal domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus envelope</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the importin beta family. Importin beta-1 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040239672.1">
        <property type="nucleotide sequence ID" value="XM_040383738.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VZ07"/>
    <dbReference type="EnsemblMetazoa" id="ACOM029299-RA">
        <property type="protein sequence ID" value="ACOM029299-PA"/>
        <property type="gene ID" value="ACOM029299"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON009921-RB">
        <property type="protein sequence ID" value="ACON009921-PB"/>
        <property type="gene ID" value="ACON009921"/>
    </dbReference>
    <dbReference type="GeneID" id="120959942"/>
    <dbReference type="CTD" id="35336"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_013062"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON009921"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033126"/>
    <dbReference type="Proteomes" id="UP000075882">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000027">
        <property type="entry name" value="Importin subunit beta-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058584">
        <property type="entry name" value="IMB1_TNPO1-like_TPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040122">
        <property type="entry name" value="Importin_beta"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10527">
        <property type="entry name" value="IMPORTIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13513">
        <property type="entry name" value="HEAT_EZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25574">
        <property type="entry name" value="TPR_IMB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="10">
        <location>
            <begin position="22"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="9">
        <location>
            <begin position="410"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009921-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="879" mass="98021" checksum="4FCB89BE68EE86B3" modified="2020-08-12" version="1">MMHIVQILEKTVSPDQDELLAAKNFLEQAASTNLADFLRALSDVLVYPGNSTVARMAAGLQLKNHLTSKDQTIKQQYQDRWRAFPEDVKEYIRKNILGTLGTEESRPSSAAQCVAYVAVADLPVHQWPDLMQKLVDNVVNEKSTEALRESTLEAIGYICQDINSEILEHQSNQILTAIIHGMRKSEPSNHVRLAATNALHNSLEFTKANFEETAERNYIMEVVCEATQSTDTQICVAALQCLVRILTLYYQHMEAYMAQALFPITLEAMKSENEQIALQGIEFWSNVSDEEIDLAIEAQEASETGRLPNRVSKYYARGALQYLVPVLMEKLTHQEEFDDEDDWNPAKSAGVCLMLLATCCGEEIVPHVLPFVNANIKSTNWRFRDAAVMVFGSILSALETDRLKPMLEQAMPTLIELMYDESVIVRDTCAWTFGRICEVIPEVAIKEVYLEPLLKALLNGLKAEPRVATNVCWAFTGLSDAAYEAVNIEDDPPQTYCLSKYFDFIITSLLEATDRPDGGQGNLRSSAYEALMEMVKNSPQDCYVSVQRTTMVILERINHVLQMESHISSHSDRHQFNDLQSLLCASLQSVLRKVDAKDAPQISDAIMVALLTMFSSSSGKVCGVQEDALMAVSTLVDLLGEEFIKYMDAFKEYLYMGLKSHQEYQVCCTAVGLAGDICRGLKSKILPYCDDIMTLLLQNLSNPNLHRSVKPQILTVFGDMALGIGPDFKKYLNVVLPMLAAATQVQIDQHDYDMIDYLNELRESVLEAYTGIVQGLKGSDKQPLEDVNALLPHVPFIVSYIVSIAKDSELSDGNIAIAAGLIGDMCGAFGPMMLQFVEDPSITELLNDGKNSRTNRTKTLAGWAMREIKRVKNLPVNSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VZ39</accession>
    <name>A0A6E8VZ39_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Gustatory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Gustatory receptor which mediates acceptance or avoidance behavior, depending on its substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the insect chemoreceptor superfamily. Gustatory receptor (GR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VZ39"/>
    <dbReference type="EnsemblMetazoa" id="ACON009256-RE">
        <property type="protein sequence ID" value="ACON009256-PE"/>
        <property type="gene ID" value="ACON009256"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007990"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON009256"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050909">
        <property type="term" value="P:sensory perception of taste"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013604">
        <property type="entry name" value="7TM_chemorcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21143:SF121">
        <property type="entry name" value="GUSTATORY AND ODORANT RECEPTOR 21A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21143">
        <property type="entry name" value="INVERTEBRATE GUSTATORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08395">
        <property type="entry name" value="7tm_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="38"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="140"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="171"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="265"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="371"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009256-PE"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="397" mass="45521" checksum="C4FF225711FCC0DC" modified="2020-08-12" version="1">MLNDKVVNGLLLFSTTIATLLGYLTITYDRKRQELRVSTVLVVYSIVLAILCTFTYQSAQFLSSDMRYRNGIKESAVNKAIGAIQGQVVLQTFLSAVVRRYHKRFEIAQLMRDLFQLKRELFSATELSQRWIKGHILRKITISQLIVTSSFVLAVLAVLDAGEETAIGSELLSLCIFYYPKLTIICSVSLYYAVMLFQQNLHYALNERLKQLIVEHTGGLADRARGYARLQHAVYVRSKLNYLADARCRIVACCTETHNLYQQMLLSCSFLCMTFIVTQLLYLHYRTRSELSFAALGHETFTCLLCYFEVYCIAEACELVREQSLKTQKLLLRLNLSPMDHKLKQSIEVFALQTLHQPIEFTACRMFTLDYTVLFSIAAAVTNYLIILIQFEMAIEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W091</accession>
    <name>A0A6E8W091_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Mitochondrial uncoupling protein 4</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8W091"/>
    <dbReference type="EnsemblMetazoa" id="ACON009603-RA">
        <property type="protein sequence ID" value="ACON009603-PA"/>
        <property type="gene ID" value="ACON009603"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004369"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON009603"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038997"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000062">
        <property type="entry name" value="mitochondrial uncoupling protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050391">
        <property type="entry name" value="Mito_Metabolite_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45618">
        <property type="entry name" value="MITOCHONDRIAL DICARBOXYLATE CARRIER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00784">
        <property type="entry name" value="MTUNCOUPLING"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="22"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="126"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="227"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009603-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="324" mass="35479" checksum="3C17D0F65956021E" modified="2023-06-28" version="2">MQQTAAATGRTEMRPVKYHYADSFWCTYLVSVFAASIAETVTYPLDLTKTRLQIQGEAAATAVDAEGALKYRGMFATATGIIREEGALKLWQGITPALYRHLVYSGVRIVTYDALRKKLRNGKETFSLWQSALSGVGAGALAQWLASPADLVKVHVQMEGKRRAMGLEPRVHSAAHAFREIVSRGGVFGLWKGSVPNVQRAALVNLGDLTTYDTVKHFIMHKTGLPDCHVVHIMSSICAGLVAATMGTPADVVKTRIMNQPTDSSGRGLLYKGSIDCLQQTIGKEGFFALYKGFLPVWIRMAPWSLTFWLSFEQIRASLGASGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W096</accession>
    <name>A0A6E8W096_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">glutathione transferase</fullName>
            <ecNumber evidence="2">2.5.1.18</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the GST superfamily. Sigma family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8W096"/>
    <dbReference type="SMR" id="A0A6E8W096"/>
    <dbReference type="EnsemblMetazoa" id="ACON010404-RB">
        <property type="protein sequence ID" value="ACON010404-PB"/>
        <property type="gene ID" value="ACON010404"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_003068"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON010404"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039261"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004602">
        <property type="term" value="F:glutathione peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03192">
        <property type="entry name" value="GST_C_Sigma_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03039">
        <property type="entry name" value="GST_N_Sigma_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000030">
        <property type="entry name" value="Glutathione S-transferase S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000035">
        <property type="entry name" value="hematopoietic prostaglandin D synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050213">
        <property type="entry name" value="GST_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11571">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11571:SF224">
        <property type="entry name" value="HEMATOPOIETIC PROSTAGLANDIN D SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14497">
        <property type="entry name" value="GST_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01205">
        <property type="entry name" value="AMPS.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00363">
        <property type="entry name" value="AMPS_(cytGST):_Alpha-__Mu-__Pi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="6">
        <location>
            <begin position="2"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="7">
        <location>
            <begin position="81"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010404-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="203" mass="23279" checksum="403F5B0F0057BC28" modified="2020-08-12" version="1">MPDYKVYYFNVKALGEPLRFLLSYGNLPFDDVRITREEWPALKPTMPMGQMPVLEVDGKRVHQSLAMCRYVAKQINLAGDNPLEALQIDAIVDTINDFRLKIAIVAYEPDDMVKEKKMVTLNNEVIPFYLTKLNVIAKENNGHLVLGKPTWADVYFAGILDYLNYLTKTNLLENFPNLQEVVQKVLDNENVKAYIAKRPITEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W2C0</accession>
    <name>A0A6E8W2C0_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Neuropeptide-like 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_040234641.1">
        <property type="nucleotide sequence ID" value="XM_040378707.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8W2C0"/>
    <dbReference type="EnsemblMetazoa" id="ACON010366-RB">
        <property type="protein sequence ID" value="ACON010366-PB"/>
        <property type="gene ID" value="ACON010366"/>
    </dbReference>
    <dbReference type="GeneID" id="120956898"/>
    <dbReference type="CTD" id="100529111"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009530"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON010366"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031259"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026151589" evidence="2">
        <location>
            <begin position="31"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="139"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="250"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010366-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="453" mass="52467" checksum="0C24521D5B73E274" modified="2020-08-12" version="1">MVRCEMILPPKLFFVRASLLLFLVLHNVTSQNYEGRRLKPCELESLLRDLTYPEEGYEMHAVALRNQLRHILEHDPSDRENYQNLEDSSEATRGPYEDKRSYRALLRDGAVFGKRNVGALARAGLLRNQPEYLKRSLATLAKNGQLPSRDTESEDTTPDGEWNEDKRNVASALQSGYMLNGKRNIASLARKYELPGKRNIQSLLRTGMLPSIAPKRNIQSLARENVLTGYGSIYKRNIQTLVRDWSLPKQHNKLSESSGSTSDNEKRNIQSLKNAQGRKKRSLYYDEMEESSPEVLEPVFQTAPLDYEELMDAMVQTYPLHNTDNHLEEKRFLARPRSRANITPDPSHYDNQPAAYVDNAYMDSEVPLVGELQGYSPRGVPRRLELPRYTGEAFASHPYVHDEFSERVNPFYTDDEYRCANRLRKLLYYLKKQYYLDNKYIQATYGRNLGYYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W4C1</accession>
    <name>A0A6E8W4C1_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="10">RRM domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RRM IMP/VICKZ family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8W4C1"/>
    <dbReference type="EnsemblMetazoa" id="ACON012103-RA">
        <property type="protein sequence ID" value="ACON012103-PA"/>
        <property type="gene ID" value="ACON012103"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012373"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON012103"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038665"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22400">
        <property type="entry name" value="KH-I_IGF2BP_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22401">
        <property type="entry name" value="KH-I_IGF2BP_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22402">
        <property type="entry name" value="KH-I_IGF2BP_rpt3"/>
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    </dbReference>
    <dbReference type="CDD" id="cd22403">
        <property type="entry name" value="KH-I_IGF2BP_rpt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.210">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10288">
        <property type="entry name" value="KH DOMAIN CONTAINING RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="17"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="92"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="515"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="533"/>
            <end position="568"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON012103-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="568" mass="60978" checksum="92BCA89CA5A001C0" modified="2020-08-12" version="1">MEFWTPLDGQQYHSTTAKIVISGIPQHAKFDDIEPLLKPYGKVEHCEAGTSKDPNTQTVHITFENHDQAQRAVAGLNGVDFDGSKLLVELSDSNKQARRAQRNRSQYGAGMPGGGGPGRQTDFPLRVLVQSEMVGAIIGRQGSTIRHITQQSRARVDVHRKDNVGSVEKAITIYGNPENCTMACKKILEVMQQEANSTNKGEICLKILAHNNLIGRIIGKSGNTIKRIMQDTDTKITVSSINDISSFNLERIITVKGSIENMSKGESQISAKLRQSYENDLQALAPQTIMFPGLHPMAMMSTAGNGMGFTGRTAGATASASGSGATGMYPGSNFPMYQPPAGGPAGVPPGSSEVQETTYLYIPNNAVGAIIGTKGLHIRNIIRFSGASVKIAPLEADKPQEQQTERKVTIIGTPEAQWKAQYLIFEKMREEGFVSGTDDVRLTVEIFVPSAQVGRIIGKGGQNVRELQRVTGSIIKLPEHTASAPVDEETTVHIIGPFFSVQSAQRRIRTMMLATNPPPVTKRQTSQKAKDQTTGGATTPAAGATAATTTTAAVTTTPPQQQSSSTNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W4F0</accession>
    <name>A0A6E8W4F0_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Long-chain-fatty-acid--CoA ligase</fullName>
            <ecNumber evidence="13">6.2.1.3</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoAs for both synthesis of cellular lipids, and degradation via beta-oxidation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(5Z,8Z,11Z,14Z)-eicosatetraenoate + ATP + CoA = (5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19713"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32395"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57368"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.15"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:19714"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>(E)-hexadec-2-enoate + ATP + CoA = (2E)-hexadecenoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:36139"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:61526"/>
            <dbReference type="ChEBI" id="CHEBI:72745"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:36140"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>12-hydroxy-(5Z,8Z,10E,14Z)-eicosatetraenoate + ATP + CoA = 12-hydroxy-(5Z,8Z,10E,14Z)-eicosatetraenoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:52112"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:90718"/>
            <dbReference type="ChEBI" id="CHEBI:136408"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:52113"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>15-hydroxy-(5Z,8Z,11Z,13E)-eicosatetraenoate + ATP + CoA = 15-hydroxy-(5Z,8Z,11Z,13E)-eicosatetraenoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:52116"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:78832"/>
            <dbReference type="ChEBI" id="CHEBI:136409"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:52117"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>5-hydroxy-(6E,8Z,11Z,14Z)-eicosatetraenoate + ATP + CoA = 5-hydroxy-(6E,8Z,11Z,14Z)-eicosatetraenoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:52108"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:65341"/>
            <dbReference type="ChEBI" id="CHEBI:136407"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:52109"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a long-chain fatty acid + ATP + CoA = a long-chain fatty acyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15421"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57560"/>
            <dbReference type="ChEBI" id="CHEBI:83139"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:15422"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>hexadecanoate + ATP + CoA = hexadecanoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:30751"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:30752"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 13">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.3" evidence="13"/>
    <dbReference type="RefSeq" id="XP_040231528.1">
        <property type="nucleotide sequence ID" value="XM_040375594.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040231529.1">
        <property type="nucleotide sequence ID" value="XM_040375595.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8W4F0"/>
    <dbReference type="EnsemblMetazoa" id="ACON011603-RA">
        <property type="protein sequence ID" value="ACON011603-PA"/>
        <property type="gene ID" value="ACON011603"/>
    </dbReference>
    <dbReference type="GeneID" id="120955083"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009658"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON011603"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032488"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047676">
        <property type="term" value="F:arachidonate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05927">
        <property type="entry name" value="LC-FACS_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045311">
        <property type="entry name" value="LC-FACS_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272:SF107">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 13" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2 13" id="KW-0436">Ligase</keyword>
    <keyword evidence="13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="3 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="14">
        <location>
            <begin position="78"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU369030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON011603-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="656" mass="73071" checksum="FB95FC82288DCD0A" modified="2020-08-12" version="1">MSCCGSQEPIRPPINPGFQSDILKGPEQVHVSKFYKEAKDGKFVSCLEEDIRTLYQSFRKGAYTSNNGPCLGWRENLQSPYQWMNFNETLLRAKNFGSGLISLGLRPGPSTFIGIYSQNRPEWVLFEQGCYCYSLVVVPLYDTLGPDACAFIVDQTEMQVVVVEDDKKVNLLLDKAPRSLRKIVAIKEVRPATLQRAKNVGIDVHTFDEVEKLGAISDNPELEPKPDDLCTVCYTSGTTGNPKGVMLTHANVLAGVAGVLLQLGPHRPRVGDIMVSFLPLAHMLERCCENGVYYMGGAVGFFSGDIKELTNDLKALKPTLMPAVPRLLNRVYDKVFAEVQRSSVKKLLLNMALSSKEAEIKRGIVRKNSIWDKLVFRKIQEGFGGRLRLMVVGSAPLSEAVLSFSRAALGCLICEGYGQTECTAPITLTVQGDFVAGHVGPPVACNGIKLVDVPEMEYYASQQQGEICVKGANVFIGYLKDPQRTAETIDKDGWHHTGDIGQWLPNGTLKVIDRKKHIFKLSQGEYIVPEKIENAYIRSQYVEQVFVHGESLKSCIVAIVVPDVEVIKSWAQENNIPGTLSVLCNNPDVKQLILNDMILWGKEFGLKSFEQVKDIYLHPDPFSVQNGLLTPTYKSRRPQIRSYFAPQLDDMYKHLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W4F4</accession>
    <name>A0A6E8W4F4_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">CUB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">Ngousso</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8W4F4"/>
    <dbReference type="EnsemblMetazoa" id="ACON012280-RB">
        <property type="protein sequence ID" value="ACON012280-PB"/>
        <property type="gene ID" value="ACON012280"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON012280-RC">
        <property type="protein sequence ID" value="ACON012280-PC"/>
        <property type="gene ID" value="ACON012280"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_003645"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON012280"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_029428"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042333">
        <property type="entry name" value="LRAD2/Mig-13-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24652:SF69">
        <property type="entry name" value="CUB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24652">
        <property type="entry name" value="LOW-DENSITY LIPOPROTEIN RECEPTOR CLASS A DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="243"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON012280-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="343" mass="38069" checksum="DBABF3ADE8682E1A" modified="2020-08-12" version="1">MAATSEVTSCGPGRPPPRERTGSSSRRGKLCQSRWMQLGTVWVTLLLVLLTVGHLGSVHGEKTKTYYMESICKNHFLQLLYRKIDGATLLSRNERNLNCVVTFQTHSILQRFMLRFDMLQLDCNDHLYVYDGAHAVGTHKADLTCKDTKQSVGALFTKTNFLTFKYVTDNWGTETNGFKMVITSVKDSKLNCADFRCKVPDFCIHQDLMCDGVSHCADGSDEAVGSLCPGPDRYINTIFGIDLTWVILIGVSSLIVCGCIIGITICVYVRNARNTPNQLNSSIQLNQMIESNGSSKHLQGTLPRETTTIPASGNLHHPAGPLGYLRIPHKLMAKVRPIWCLAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W6E8</accession>
    <name>A0A6E8W6E8_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="9">TMC domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMC family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8W6E8"/>
    <dbReference type="EnsemblMetazoa" id="ACON012019-RB">
        <property type="protein sequence ID" value="ACON012019-PB"/>
        <property type="gene ID" value="ACON012019"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_003823"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_013223"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON012019"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030626"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042609"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008381">
        <property type="term" value="F:mechanosensitive monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038900">
        <property type="entry name" value="TMC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012496">
        <property type="entry name" value="TMC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23302:SF24">
        <property type="entry name" value="TMC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23302">
        <property type="entry name" value="TRANSMEMBRANE CHANNEL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07810">
        <property type="entry name" value="TMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="86"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="234"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="327"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="450"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="490"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="520"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="578"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="641"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="684"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="740"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="domain" description="TMC" evidence="8">
        <location>
            <begin position="569"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="810"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="847"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="870"/>
            <end position="883"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON012019-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="883" mass="100295" checksum="B143974EF9CC0861" modified="2020-08-12" version="1">NLINEERTWEEIREIKSMPVPMAQKREMKAQLQNATKLRLQGFEQIKWRRRKAWQSFRSKWGEYQTKLELWRMSLKTIEGNFGTGVVAYFLFLRWLVFLNLLLFLLIFAFVVLPHLVLREPTDLPCDLVTDPTSVQCCSESYENVTRTAQFSVLDLIQGTGFVEGTLLFYGMYTNQIYGYSPMEDRAYHTARQLRMSAAAAAAINGSTVETSRLLESDLPDPERQTILYYDLPLVYVIIIAIYYIITLVAIMRAVVRQFKDRIAEGEGLFYQYCNLVFGGWDFCIHNQKSADIKHRALHNEIKSLLYQKRFEHERNNRSREFMLKLIAIRMLVNLVVFVILLLAAITIYVLFNVSLAELEPNFTPSHPVSFSEGIGNRTLYMSWPSSWSVFSSASQRGAISFASTITPLLSSISDEVGAGGGGSGGFNATDPTMERLSLEEKLRVLFYEFLPYLAIVCMNLVVPQLFNYLVQYEKYSPLFVIKISLFRTVFLRLSSLAVLLSRFYFLITPMNLPEANASLVANLTASTISVLGTLLLDRQSPVSEAQTQQQQLQLPTQCYDEQNGAPQCWETFVGQQFYKLFIVDFATHFLVTFFVNFPRALFARHSSSRLAKFIGEQEFELSKHVLDVIYSQTLCWLGTFYAPFLPAIAALLTFLMFYIKKFACLVNSNPSAILYRASRSKSLFMSTLLISFTLALVPVVYALAEIVPSRSCGPFRGLPSVWDRAIAAFMKMPQFFQNVIFYFGTASFAIPCFVVLTFFIYYYYAVSTANRHMVQVLKQQLVLEGHDKQFLLSRLSLFIKQQQDNTANIVIPPPSAYQQQQPPVVPPPRTNSQSQASEPTPALPPRADRDSKPSSRDRPKDLPPPPIPASGSSSTGNTTGAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WCB5</accession>
    <name>A0A6E8WCB5_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="5">DUF4758 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8WCB5"/>
    <dbReference type="EnsemblMetazoa" id="ACON029559-RB">
        <property type="protein sequence ID" value="ACON029559-PB"/>
        <property type="gene ID" value="ACON029559"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002507"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON029559"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037153"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd00037">
        <property type="entry name" value="CLECT"/>
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        <property type="entry name" value="CTDL_fold"/>
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    <dbReference type="PANTHER" id="PTHR22803">
        <property type="entry name" value="MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED"/>
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        <property type="entry name" value="Lectin_C"/>
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        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026153649" evidence="3">
        <location>
            <begin position="17"/>
            <end position="351"/>
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            <begin position="223"/>
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        <location>
            <begin position="87"/>
            <end position="149"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
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            <dbReference type="PROSITE" id="PS50041"/>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="351" mass="40911" checksum="AB82529FE7BA5439" modified="2020-08-12" version="1">MVRLVVLLLCVSMATTQKPGNFNFKEKGFKSSQSYDIDPQVLEIQRQNLITQKILQRYLERRLHPGRKPQIRVPSVEEILPKPFSPLASVRDVRQQNQTESDDNGGARYDLFNGQNGRQYNRSEEDGGGGGSSQDEQQYNDEGGDDYREERDLDQYDDLGDESTYDNDRFDIESLQSGESEHVDSFYRDKYYSRQRASQLSDCPNCVEESSAPPRWTMPLLKLGEKRYYLSIFFKANWFKALQYCRFHGMQLASIQTQEENDRLEKYVKDYGLATEHFWTSGTDLAEEGSFFWVSNGRPLSFTNWNAGEPNNFRYENGEEEHCLELWNRDGKGLKWNDTPCSFETYFICEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WCB9</accession>
    <name>A0A6E8WCB9_ANOCL</name>
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        <submittedName>
            <fullName evidence="2">Serine-rich adhesin for platelets</fullName>
        </submittedName>
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        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
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            <taxon>Metazoa</taxon>
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            <taxon>Insecta</taxon>
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            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
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    <dbReference type="AlphaFoldDB" id="A0A6E8WCB9"/>
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        <property type="gene ID" value="ACON029561"/>
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    <dbReference type="VEuPathDB" id="VectorBase:ACON029561"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042479"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        </location>
    </feature>
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            <begin position="24"/>
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        </location>
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblMetazoa" id="ACON029561-PC"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1118" mass="119648" checksum="FF707E0315048262" modified="2020-08-12" version="1">MRNNHIDFVALDNILKSVADQPCTAATTPPHSNPTHSKSPPKASFLTAESLREIRGRLRRLSNESLYRDDMSPADDASGQTVASDRPSPADEGGVTITEVETRLGQLETSSSSDSNYRSSSLQTSKKPAPDTTVLAPDDWHSRRKSYGFERMSQQPLGSGFAMAKMDSSTDSGIGRSSDLSSSWSSAATESGQGAGGSYHHQRATIVTLGERSQLKEKQATNPAMVIKITSKPADSTSDTFAVPSLEPTDEEPKRHSIAVDETSYVRDSLRTMFERKSSVHVNGFSHTLIDDLSRNKKRVEFCKTEVHFTADSGRVNIVETDEKPPPTNNFRRRRRSSGGSSYLVSNLDGDSDSVNATDESKLVGPSSESVVIEQDTAPVVTTTIGGMGRTVPEPTPRKYVPSATVTVSDESDGHSTDEISLRSILKNKPVKPKPYVLGENLDSNEALWGVKLKPVVAGGATVEQSQPRLLSVEMGTESKSSSPIAAGSGYSTRINLTKEPAGPSSWSGSNSTEFDVVKPSALISRQPAKPSAVMSIELKSPTPVKKLPPASHTSSPDTSTTKITTTLNDESTAASTQQQSPKFRRYIPTTATSATTPGAVRNEERASLRLSSGARQLRENELSFFGVNPNKQQSSSPAGPTGKPPTTSTTTPNSPFQRSATLTSSFIRRSGTTTTSSTTTTTGSGDSATSKTSLWQLTNDKPDLLRHGQQAAAAVEQPKSQAVAAPRPAVIRTGSSSVATSNSVSVNSSSSSVTTSTLSSADGSPHYENLSHFALKPKAAQRSVGSSPSAGELLVQQQTAKVAESEKPAAAYDRKKDLERDERILEELTRAADEILNVVNNMSNGESMESILSELNDGEGHYRRLSTGGCTLGTIRECSTTNKIMKSRGVQVSKNIDDSLKRHHQRQSRLSSASSNESIGRRPEGNFTRSVSLRGPSTASTTAGDSLSSRSGSSRRSRAPMATETRRLVRMCSKEKLQSNASSSEDLPSTTVVEPPRRPRRTRYHSKETKDPERTSGTSNGHGTRVSSSTRSHRSERSSGSIRTVSSRHQGQSATPTTGHTAQTAVSASVSSHHSLHSASCPSSRGLKSSATNGHHQHHHSQLASATTTTSSKKHHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WCC6</accession>
    <name>A0A6E8WCC6_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Tensin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PTEN phosphatase protein family.</text>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON029568-RA">
        <property type="protein sequence ID" value="ACON029568-PA"/>
        <property type="gene ID" value="ACON029568"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012720"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON029568"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_043036"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01213">
        <property type="entry name" value="PTB_tensin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09927">
        <property type="entry name" value="SH2_Tensin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013625">
        <property type="entry name" value="PTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006020">
        <property type="entry name" value="PTB/PI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035012">
        <property type="entry name" value="Tensin-like_SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014020">
        <property type="entry name" value="Tensin_C2-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033929">
        <property type="entry name" value="Tensin_PTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051484">
        <property type="entry name" value="Tensin_PTEN_phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45734:SF10">
        <property type="entry name" value="BLISTERY, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45734">
        <property type="entry name" value="TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08416">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10409">
        <property type="entry name" value="PTEN_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00462">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01326">
        <property type="entry name" value="PTEN_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51182">
        <property type="entry name" value="C2_TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0727">SH2 domain</keyword>
    <feature type="domain" description="C2 tensin-type" evidence="8">
        <location>
            <begin position="292"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="7">
        <location>
            <begin position="1402"/>
            <end position="1508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="40"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="120"/>
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    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="501"/>
            <end position="543"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="569"/>
            <end position="662"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="685"/>
            <end position="753"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="776"/>
            <end position="801"/>
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    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="839"/>
            <end position="863"/>
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    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1052"/>
            <end position="1077"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1146"/>
            <end position="1332"/>
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        <location>
            <begin position="1"/>
            <end position="14"/>
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            <begin position="42"/>
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            <end position="581"/>
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            <begin position="644"/>
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            <begin position="704"/>
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            <begin position="1320"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00007881"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
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    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51182"/>
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    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON029568-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1677" mass="184916" checksum="BD4B7D52421ABC22" modified="2020-08-12" version="1">MIRVNSQSFSLSSTLKKKNGREEEQQTINKYNSLPLHTINAQQQQQQQPRPTTNLNHVNNNHYHNYSLQPYYNRQQQQHHQQQQQLQHHSYPVARIDEEPAAQSLRLNYVTERILASTLPARRLQNGSSSPHAPNGTPPVDEHERELINMLEQKHKQNYRILDLEARLVNITLEKLCELCKYIDSWLGSGKEKIVVLQDREDKHRLGTAIAAYLQYQKICGSNIPQASGHSKNSLSPGGTDNGRRTPTWLDLDIYSMQKFLETVAGPLRIPSHRRYIQYFSGLLSGVIKLNSSSFFLKAIRVESPPCLHYRAITVNSEWRSFIKIFEGVRCLFTSDIYVIPITTRHFIYEIKHPLRLRGDILVRCYQIIPNNNKATYEKELIASVQFHTCAITEKEVQFTKGDVDFACEDERFSAEHRMTFCFDTVPNERPMILVFQDPLVRIEPIAENGDLLETINEDESGHTQGPLDGSLYATILKSPKSPTATTHLISPPAEFSNGSSKSLLLLNGNGPPPPVPERSKTPNSIYLSQNGTPRSTPVPFALAPPPAASPAFGDRSVRAVSSASNSVSVNSSYSSYQSASPGVATVPDGGSPGATLATPGGTKARPPSAQQVDGRESVRSPLTVSMDSGISSSGPVNRRVQGSSVSPSSFPSPQASPQDDRHRELDDLLSDMMLTVQGIPDVGQKADQTDHHQSQQPQQQQQHQHHHHHHHHHHQNPNDHFVNTNTDTIKRSHSAQLPQREDARSRTPLTAVSDYSPATAAAAAAKERELLMYETSSTTTTLTPPPSESGRETPLLSGPSYAAAAAAGTIRERELQNINNNNLTPAERELIMSVQRHQHQSLAYPRPARSTTGASERFTSDDDDDLLVHGGTGSVAQIPYHAREDSRPFTYGNIPATGTPQQPPAATMLKMQSGLSSPSMVRKALGTPTASRKTAGTLPRNDFEEMLRERREKVLSEKYTIGDQSPGGGMMVSDTINNNSSGDGRWTYQQHHTVKTVTTQGGGGAGTPNGYPSHEPLKRSNTMDGSFGRQFSNEGISGQSWLQLQQQKLRARREQQRREHSNSFSYNYGSPAFPTAGENAYSTTHRRSQTLSPVRNERNYHTLTTTRTHSTERPFVAVQRAHDNAKLQTIGNAPLSILNASPHGHIAQAQTAPSSSTPSPSAVSSPSPVPTANGNTTTTSSSSTNNTSVSITHHHQTVNGGGSPVNGHATPQPQHRHVNGNGAHYQQQQQQQQQQLNNSLNGSSGLLSLAEQDHSTPKHGQVRLAREESFSSYRSETEPDNSPMGGSPRPETPAFPVTPRTPYGLSNGTSSPALPPKSPTSQRRYNSAWSLRSQKSSVSTYDVSKDLFSGNQQRTQELVNQNETLSCYTSRRNSTTSNANSEPQEVAPQFVKFARDSSKYWYKPNISREEAIALLRNAAPGTFIVRDSTTFANAYGLVVKVNHPPPGVQYTGPNSEELVRHFLVEPTIRGVRLKGCANEPVFTSLSALVYQHSITPLALPCRLIIPDMDLQQMEHQTPAQQQLLQQGAACNVLYLFTCDTESLTGPQAIRKAVSSLLALRPLPKPTQVHFKASLQGITLTDNTRQLFFRRHYPSNNVSFCALDPDDRRWSIQSTTGDIPASKRMFAFVAKRSPSSADNQCHVFCELEPTQPAAAIIQFANKVLSGTSQQPAVTRAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WCF4</accession>
    <name>A0A6E8WCF4_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Kinesin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_049466640.1">
        <property type="nucleotide sequence ID" value="XM_049610683.1"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON029594-RA">
        <property type="protein sequence ID" value="ACON029594-PA"/>
        <property type="gene ID" value="ACON029594"/>
    </dbReference>
    <dbReference type="GeneID" id="120959964"/>
    <dbReference type="CTD" id="34422"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004264"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON029594"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_036234"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005875">
        <property type="term" value="C:microtubule associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051231">
        <property type="term" value="P:spindle elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01372">
        <property type="entry name" value="KISc_KIF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22248">
        <property type="entry name" value="Rcc_KIF21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000011">
        <property type="entry name" value="Kinesin family member 21A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001563">
        <property type="entry name" value="Kinesin protein KIF21B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056532">
        <property type="entry name" value="KIF21A/B_hel_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969">
        <property type="entry name" value="CHROMOSOME-ASSOCIATED KINESIN KIF4A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969:SF15">
        <property type="entry name" value="CHROMOSOME-ASSOCIATED KINESIN KIF4A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23203">
        <property type="entry name" value="KIF21A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25764">
        <property type="entry name" value="KIF21A_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="9 12" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 11" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Kinesin motor" evidence="15">
        <location>
            <begin position="9"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="1319"/>
            <end position="1358"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="1509"/>
            <end position="1554"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="458"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="560"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1014"/>
            <end position="1050"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1092"/>
            <end position="1122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1137"/>
            <end position="1156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1233"/>
            <end position="1276"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="366"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="601"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="665"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="833"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="458"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1014"/>
            <end position="1027"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1035"/>
            <end position="1048"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1142"/>
            <end position="1156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1239"/>
            <end position="1254"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="88"/>
            <end position="95"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON029594-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1640" mass="179945" checksum="5D47198909CA7696" modified="2020-08-12" version="1">MPDEDKESSVRVAVRIRPQIPRELIDMCRVCTQVTPGEPQVLLGSDKAFTFDYVFDMSTTQVSVYNNCIEKLVDGALQGYNATVLAYGQTGSGKTYTMGTGFERALPEAQEGIIPRAVRHLFEGIAQLQQNPYDEDGTYLGTVTFSVAAQFMELYNEEVIDLLDPYNKGARVFKIFEDATGGISVAGATIKPLAGPQEALNCLQQGALARTTASTQMNEQSSRSHALFTILIRRQRVMTAEQCGNAEGDTETLTSKFHFVDLAGSERLKRTGATGERAREGISINCGLLALGNVISALGDKTIKVSHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVQINQDQSSRTISLLRREIANLQLEILEYKQGKRSIDADGNIAISDVSLENEMLSQDNKRLQQRVKAMQETINALTEKNAALQAQQTIASLHKASAAGDGGADAASTNNGSSPVGTAGGNDSAMQELIVGYISEIEKLKAKLIESEQMFQQFKKAKSSQSKLGIKTYPFIEDNPETMINMLKHEMEKERETLMSRSLPGLEHESSSMDQNSDSDSDTESDDKAEVLRAEMSDVNSDIELKVRLIEQLEESQQRLQIMRQQYEKQFNLMKEKISNTERERDEVLATIGNGGTGAMNNKGQNSGNETAIKRVKEDYERKLNELRRQLNHFQATNKEHLRLQRNMQAQDAKIKTLRGELAELKQVKTRLMKKIQEESNRHKEMESRKTREIAQLRKETRKHKNMIKSLQAQGAAKDQVLKRKTEEVFNLRKSQRGIMSLKAAGRVQGNTSIGSMLQGTKRFKSRWEELQRSIMRAARTRQAVLELEMELERVMQERDVLSRDLTNLRQRRKDNAESTLQDLVSEEDTIIANMNYLHDTITELQKSIIQIEDGKDLNSEHLMLQTIMENIGTVEEAKFMLQRVCTVTIGHVCEAGVAQSKLRERDALLTELQRDTSVQEQLLQYILTRAPAASASDASFSSAQSANSYATQSQANLLENGEPQPSSSQQYRLPHVVDSTTRSPSPSSSNTNLDSIVTYRNSSKQRRNPSVVPSVATIQDLLYGSSSSYVGPAGDGAGGVGIPDRNLNHSAGNGGTGASNGASSGGSKLEKVGICIYLEDSADDDDTKHPPSLQDRHERSDVMTRSYTILDGAMDAVAAANAGLPIAPPPPVAPVPARTFVPLSRVPSAPGSLKGLQPHQGLSRQNSTASPLLARKSFEASGAPSSPRISRRTFTSKMSPGIEDSNDVPTSPPVYRRGISREDNGDVFSRLGAGTQDPQPGGNIKELNGRYKAGSPLICTHVVEGHTNSVLSIKVSNQMLFTAAADRTVKVWDLRTNSTPHCLTGHLGPVAAVEYDRVNNLLFSASGAFVKVWDLRSSNIRPIITLCSSGTTLPANATLSDLVPGECSITALTMGASGKLYTAASDKVRFWDLRQFSSLGRLSGGHQAAVMCLTAWEGPNNTDLVATGSKDHYVKVFEVNSSGGVVQPLLNLEPPHYDGVQALAVANDAIGVDAELFSGSRDSGIKRWDLRNGELKQSLNNAHKGWVSGMAIYGDILLSSCRGGVVRLWNIKTCDSLAEMKTEQSINDIVTSDNRVFTASNDGKVRLWRVSSAIRRLGPNETSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WEC0</accession>
    <name>A0A6E8WEC0_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">TSC22 domain family protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TSC-22/Dip/Bun family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8WEC0"/>
    <dbReference type="EnsemblMetazoa" id="ACON029804-RC">
        <property type="protein sequence ID" value="ACON029804-PC"/>
        <property type="gene ID" value="ACON029804"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005905"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON029804"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037488"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008284">
        <property type="term" value="P:positive regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21936">
        <property type="entry name" value="ZIP_TSC22D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.490:FF:000002">
        <property type="entry name" value="TSC22 domain family, member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.490">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000580">
        <property type="entry name" value="TSC22/Bun"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047862">
        <property type="entry name" value="TSC22/BUN_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46745">
        <property type="entry name" value="TSC22 DOMAIN FAMILY PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46745:SF1">
        <property type="entry name" value="TSC22 DOMAIN FAMILY PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01166">
        <property type="entry name" value="TSC22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58026">
        <property type="entry name" value="Delta-sleep-inducing peptide immunoreactive peptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01289">
        <property type="entry name" value="TSC22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="125"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON029804-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="220" mass="23752" checksum="3682D7989CBAFC86" modified="2020-08-12" version="1">MTSALLAKWPARRLSQLTVKNCVQLSARSSSNHHQLQATYRYKQYSMLLGLRYDEKRLLLSWLCAHNLESVSMSTPPVRRITIGGVPSKIVSIPRRISLSASGTSAVAIDNKIEQAMDLVKSHLMFAVREEVEVLKERISELMDRINQLEYENNILKANASQETLAQLTTGGSTVAAAAAVAAAAAAVAQQQLTAMPNINVGPTKVPTSSANPANPGSVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2025-10-08" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9I3AN91</accession>
    <name>A0A9I3AN91_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="4">DUF676 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2023-03" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM135 family.</text>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON005546-RB">
        <property type="protein sequence ID" value="ACON005546-PB"/>
        <property type="gene ID" value="ACON005546"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031434"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035387"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000004">
        <property type="entry name" value="Protein FAM135A isoform a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022122">
        <property type="entry name" value="DUF3657"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007751">
        <property type="entry name" value="DUF676_lipase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044294">
        <property type="entry name" value="Lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12482:SF5">
        <property type="entry name" value="DUF676 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12482">
        <property type="entry name" value="LIPASE ROG1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12394">
        <property type="entry name" value="DUF3657"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05057">
        <property type="entry name" value="DUF676"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF676" evidence="3">
        <location>
            <begin position="1691"/>
            <end position="1882"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="65"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="144"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="595"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="775"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="917"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1041"/>
            <end position="1095"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1162"/>
            <end position="1202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1223"/>
            <end position="1251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1286"/>
            <end position="1328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1348"/>
            <end position="1425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1459"/>
            <end position="1504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1587"/>
            <end position="1638"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="66"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="144"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="178"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="215"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="775"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="785"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="975"/>
            <end position="989"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="990"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1043"/>
            <end position="1056"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1067"/>
            <end position="1083"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1167"/>
            <end position="1177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1178"/>
            <end position="1193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1224"/>
            <end position="1234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1239"/>
            <end position="1249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1371"/>
            <end position="1381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1399"/>
            <end position="1409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1471"/>
            <end position="1480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1486"/>
            <end position="1500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1604"/>
            <end position="1615"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005546-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1960" mass="211808" checksum="04321FF4F1F8D9D3" modified="2023-06-28" version="1">MADLQATVEFAVELYKFYNVDLFQRGLYQVRCSLRVSPRLPVQVETCLPDATTAGIPSVQSVLSQNGCGSNSHNSNNSNNNNNNSNSNCNTINNGTSNGSVGAGSNTSGIVSGAGTTGSGSLPSSNTGSVGGGLVAIGASTGNGNAPSVGSASTTGRPDVGPCLSSTASSGVIGGGSMSSMPSSSSMPTPNSAITTGSSIAPGSAPGPGCSNGSSGAGGGGGGGGSSGNSPTASSDSRSGATIIDDTGASRVFQILYRNEEVSLRDVIMFRAHLLVDSRHLKESIERAEFSLNLELWFGEQTNGNVLTLASTRTLQLNFHPGRGLHYHLPVLFDYFHLAAVSVGIHASLVALHQPYINAPKTTKPWMSSSKLNCRGGNSPGPLEAIFFGPQVGSGAKCGGGSAGRLLHARNVHKEICALLLGALESLRLSLQEFCTVLPHQWTALTGSTPLSNLDTEQRLRKLSDSAKLLDTEDDLAARANSDIAQLCAECILYWRRVLSAATQPAVHGLLAKKHHTLRVRRFAEGFFVIDNPRHLASGCYDSNYQNYVSICEMARRSRYLSSLPPLPVHCTPLDGDANSLPLIFEDRYTDANEYGKRRSGSEPHLNGTGALASASSTGTLVKPAKQHHHPNKIAPIMTNTSKECSCGIATCYVEPVPKICGSYMGIMTPRYALGGDILQASLTITPAAAASQNGLCPKALSRHSVSTLLDPVPLTEVCPWDQQFKGIYTERGPSSGLAGGTLPTRHSKSLDQLEIQPNANSASLPRQHLHGTATVTNYNGYGTQQHQQQPPQQHLQQQQQQVGPPLHHQPQQRQPMMYQHPGQQTQQQLAGGIALLQTLPKSKPKNPVQAEDLLKNINEFREKYKNPGDPRKMIHAGGTATHNPTYDLQQDALGMSALLNGYAKPTTTGDYRHELKQSQQGMHNAPHHNNHRDQGHGGGPIGSTPAGSYYNSLPKGAGMGAPRSSFPPVRSKKQQQSSSTSLPTKSASNTIPRSNSSQQLRQSTQNGAQSGGAGGTVNSATLPHRSVEFSRIRVADFKQLVHGQQQRRSTSNGSTHKIGGPGSGSAGTNVGRDSSSSATSPEITPRTRNKQRRLLSSASVPFQLENLELEPVGDAVPGVAGVVTGAGGFSESLPNLAPPPAAFASDSPPGPVGGATFLLRRRHSSSESTSSLSEQSGWVSSRRSTGPSSPDTLRNDQRVVNGEQLRKRLLKLLNEQPRLVANRKNSTPSSTTEFFARSNQSSMKSKSSYAPGDMIRGASVQTENWNNLEKEWNDTRARALTSAASGPYGMVSGKRAPEPAETTAREGISTADIKPPRRTNKKEKSKSDFDLAHISDYVFDDLRLLPPQQFRDVPPPPDEFRDPPSFVDDGSSKRAKEGAKRQAAPVLPPPSNIPSQPERQKIAKEEQKQQPVPPIPQQQTQQRQQIQYYATLPKSHPPVPMTPLSVIFQPQIITAPQPQQHYATPQALMQQQQQQQQQQHHYSKPLNNQAYMGPGSTQPTPKPKCEVQAIDNPLYHIYEVVKTPRPVMKSQSSNELAAIAKCAETLNNANASNMCTVAVATQMQQAHQAPILHNGKAQYGQATMASTVERKASVRETPPTQPQPTVEPTVGTVGSVRRKVPEPDDDLDEESPDRKRVDPPMQLLEFEKCREEFRKQINYSGSIYSDFQKMASEMPYFHIADEFRAFSPAGLHLVICVHGLDGNSADLRLVRTYLELGLPGTHLEFLMSERNQGDTFSDFDTMTDRLVAEVLYHIETYQLNPSRISFVAHSLGTIIVRSALARPQMRPLLSRLHTFLSLSGPHLGTLYNSSGLVNMGMWFMQKWKKSGSLLQLCLRDAADPRQSFLYRLSQRSTLHHFKNVLLCGSSQDRYVPPHSARLELCKAAVRDQSNLGIVYREMVHNIIAPMLARQDLTLARFDVHHALPHTANALIGRAAHIAVLDSELFIEKFLLVAGLKYFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9I3BDG4</accession>
    <name>A0A9I3BDG4_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Trafficking kinesin-binding protein milt</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2023-03" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the milton family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_049464734.1">
        <property type="nucleotide sequence ID" value="XM_049608777.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9I3BDG4"/>
    <dbReference type="EnsemblMetazoa" id="ACON030706-RA">
        <property type="protein sequence ID" value="ACON030706-PA"/>
        <property type="gene ID" value="ACON030706"/>
    </dbReference>
    <dbReference type="GeneID" id="120958348"/>
    <dbReference type="CTD" id="45683"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033627"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017022">
        <property type="term" value="F:myosin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048311">
        <property type="term" value="P:mitochondrion distribution"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047496">
        <property type="term" value="P:vesicle transport along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006933">
        <property type="entry name" value="HAP1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051946">
        <property type="entry name" value="Intracell_Traff-Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022154">
        <property type="entry name" value="TRAK1/2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15751">
        <property type="entry name" value="TRAFFICKING KINESIN-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15751:SF12">
        <property type="entry name" value="TRAFFICKING KINESIN-BINDING PROTEIN MILT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04849">
        <property type="entry name" value="HAP1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12448">
        <property type="entry name" value="Milton"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01424">
        <property type="entry name" value="HAP1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01423">
        <property type="entry name" value="Milton"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HAP1 N-terminal" evidence="8">
        <location>
            <begin position="112"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="domain" description="Trafficking kinesin-binding protein C-terminal" evidence="7">
        <location>
            <begin position="578"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="122"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="502"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="869"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="928"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="976"/>
            <end position="997"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1105"/>
            <end position="1254"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="180"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="296"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="143"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="935"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1108"/>
            <end position="1137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1145"/>
            <end position="1165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="1217"/>
            <end position="1228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON030706-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1273" mass="137172" checksum="6761D5C4B4792CBB" modified="2023-06-28" version="1">MDSYDVSPKSGVSGLEEEVDKSSSTPSDFEVFDYEDIREEAAGGRNGQLPKADASRAYHRRLGTLSSDDEDEACGTPLNLELAQLLTQGIVTKAKANFGISKNIETLERSTHELELEQSFYEQQQQDHDVPTESNSKGHRSISRTASTGSYSASYKRDPDLYLRRSDDVERDYYDDAQLQQDAEEEIDVVQLKNEQLQQRQQAADSEQVGHFFEEDEMVFETHTPGSVGGSGFFRYAEPTTSYHSACGGYDSERMMYEVLCGNRVSQMTRAYDDIDAVTRLLEEKEKDLELTVQIGKELLAQNTHLENRVAELVQELKTTNENRAQLSHELHQKIELIGILTNDADDTSENVTPTASKSINLELLQRKVKQLEDENKSLRTETAQLVKETDECEEQERRLMADIANQLTTANSEFDGLNLELERLKEENRLQHEQIISLTGRLADAEIRLHQLTSENEEASSLLSITKENQNLLAGELAEFKLRYQEVLNLLQEAQEQLRRQRKRSQPLARSSLIPGITGMPAAPDSLQSELMETSLYSEHSLDSGIDSVRGGIGGGGSMMGGMMTGSQQQVPAYRKVFETVRSASRANPNGFSDSFSQLGSMTMSSSSQPRMAPYVYPGLGGTTGNGSGGSNAGITTMTHSYKSGSSVYSTMYGGGGGSSLGGRTYSRESLTADSEDGYPGPAQTGIPGAPGAKDLEAALKRLTPAEVLARRAMLQHAPLGTYSYDEQPAGIPLGCRTPDSIMSTGSSGLSGLSSSSAAGNGQWRLPEKLQIVKPIEGSQTLHHWSRLATPTLSGLLEERPGVTIRGGRGLDELGLQTYSLSDVEEDEDTEDHPGKRFQSFGCTYTYTNSTVLHPDDGTTAVTFSLPPSQMSSQMTSECPTRQPTAPSTPRSSLSRRNSCSTFSVNMGLASMLNERGIKAVTPSALNTPAGPNYSPTVTPCNSPDGSPTRSMSPEPPLLSGLLASTADILRKRFVASTGGQHSSADPADRPSRIMTRNKVALSRLEKKALRSIKIMEKVESIGLENIMLPPQHPPGISPLALHGTSALYTAAASGRGRSPMAQLTSLKHMQDQRRKAQQQQADDLVTIDKETIKAVLTKGLSHDSLKSMASSSGASSGISTAMSSDSDSSSVASFDSEPRTKDTTSGASSAATNTNTASPTTAARLKQMQRQKSRRNLLNGANGGSQRPDLGTVNGSSRPGVRPDLGTVGSKATGKTGGNSSTGGSGKDSRSKQQSTATAAAPAVKEESRSLGQSVYGTISSLLFGRKGGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9I3BDG7</accession>
    <name>A0A9I3BDG7_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Trafficking kinesin-binding protein milt</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2023-03" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the milton family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9I3BDG7"/>
    <dbReference type="EnsemblMetazoa" id="ACON030706-RD">
        <property type="protein sequence ID" value="ACON030706-PD"/>
        <property type="gene ID" value="ACON030706"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033627"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017022">
        <property type="term" value="F:myosin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048311">
        <property type="term" value="P:mitochondrion distribution"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047496">
        <property type="term" value="P:vesicle transport along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006933">
        <property type="entry name" value="HAP1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051946">
        <property type="entry name" value="Intracell_Traff-Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022154">
        <property type="entry name" value="TRAK1/2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15751">
        <property type="entry name" value="TRAFFICKING KINESIN-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15751:SF12">
        <property type="entry name" value="TRAFFICKING KINESIN-BINDING PROTEIN MILT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04849">
        <property type="entry name" value="HAP1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12448">
        <property type="entry name" value="Milton"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01424">
        <property type="entry name" value="HAP1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01423">
        <property type="entry name" value="Milton"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HAP1 N-terminal" evidence="8">
        <location>
            <begin position="23"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="domain" description="Trafficking kinesin-binding protein C-terminal" evidence="7">
        <location>
            <begin position="573"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="497"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="864"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="923"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="971"/>
            <end position="992"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1100"/>
            <end position="1249"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="291"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="9"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="19"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="67"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="97"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="113"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="135"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="172"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="206"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="930"/>
            <end position="948"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1103"/>
            <end position="1132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1140"/>
            <end position="1160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="1212"/>
            <end position="1223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON030706-PD"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1268" mass="134994" checksum="357F4464FA669D7C" modified="2023-06-28" version="1">MLSRAVNVFRKKKSRKSAHHDGEDDRHGTDTNASETETEPDDCSCHSEPVLPAGLSPAFVSNGRLLQDSSSASSTEYFQDGPEQYQETGGRRKKHSRKEEQTASDEVREECRSSSSIASSLLSTIRRARSRSRSRSIASSSPAATGRSTSKLLPTSKSTLSSRSHRGDDHGGGCGDRGYGGTGSSASSALLHGDEERNALVSSDSFARDTKRGVRDPSEDHLRSSPTQSTELAGAASGSGSGGSVRQGDGKRQVLCGNRVSQMTRAYDDIDAVTRLLEEKEKDLELTVQIGKELLAQNTHLENRVAELVQELKTTNENRAQLSHELHQKIELIGILTNDADDTSENVTPTASKSINLELLQRKVKQLEDENKSLRTETAQLVKETDECEEQERRLMADIANQLTTANSEFDGLNLELERLKEENRLQHEQIISLTGRLADAEIRLHQLTSENEEASSLLSITKENQNLLAGELAEFKLRYQEVLNLLQEAQEQLRRQRKRSQPLARSSLIPGITGMPAAPDSLQSELMETSLYSEHSLDSGIDSVRGGIGGGGSMMGGMMTGSQQQVPAYRKVFETVRSASRANPNGFSDSFSQLGSMTMSSSSQPRMAPYVYPGLGGTTGNGSGGSNAGITTMTHSYKSGSSVYSTMYGGGGGSSLGGRTYSRESLTADSEDGYPGPAQTGIPGAPGAKDLEAALKRLTPAEVLARRAMLQHAPLGTYSYDEQPAGIPLGCRTPDSIMSTGSSGLSGLSSSSAAGNGQWRLPEKLQIVKPIEGSQTLHHWSRLATPTLSGLLEERPGVTIRGGRGLDELGLQTYSLSDVEEDEDTEDHPGKRFQSFGCTYTYTNSTVLHPDDGTTAVTFSLPPSQMSSQMTSECPTRQPTAPSTPRSSLSRRNSCSTFSVNMGLASMLNERGIKAVTPSALNTPAGPNYSPTVTPCNSPDGSPTRSMSPEPPLLSGLLASTADILRKRFVASTGGQHSSADPADRPSRIMTRNKVALSRLEKKALRSIKIMEKVESIGLENIMLPPQHPPGISPLALHGTSALYTAAASGRGRSPMAQLTSLKHMQDQRRKAQQQQADDLVTIDKETIKAVLTKGLSHDSLKSMASSSGASSGISTAMSSDSDSSSVASFDSEPRTKDTTSGASSAATNTNTASPTTAARLKQMQRQKSRRNLLNGANGGSQRPDLGTVNGSSRPGVRPDLGTVGSKATGKTGGNSSTGGSGKDSRSKQQSTATAAAPAVKEESRSLGQSVYGTISSLLFGRKGGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABM2AAH1</accession>
    <name>A0ABM2AAH1_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">UNC93-like protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the unc-93 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040220028.1">
        <property type="nucleotide sequence ID" value="XM_040364094.2"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON000834.R73">
        <property type="protein sequence ID" value="ACON000834.P73"/>
        <property type="gene ID" value="ACON000834"/>
    </dbReference>
    <dbReference type="GeneID" id="120948095"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd17406">
        <property type="entry name" value="MFS_unc93A_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010291">
        <property type="entry name" value="Ion_channel_UNC-93"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051951">
        <property type="entry name" value="UNC-93_regulatory"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19444:SF13">
        <property type="entry name" value="PROTEIN UNC-93 HOMOLOG A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19444">
        <property type="entry name" value="UNC-93 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05978">
        <property type="entry name" value="UNC-93"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="32"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="69"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="97"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="121"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="158"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="242"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="303"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="335"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="364"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="390"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="429"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="453"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="491"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="491"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="503"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000834.P73"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="520" mass="57338" checksum="6F17D48CF6950753" modified="2025-10-08" version="1">MHGGGVDTDKNGGDTASLREKVQLRRGEKWRILKNIITVSVAFMVQFTAFQGTANLQSSINAKDGLGTVSLSVVYAALVVSCIFLPTLVIRKLTVKWTLCVSMLCYAPYIASQFYPRFYTLLPAGVLLGLGAAPMWASKATYLTQLGQVYAKLTDQSVEAIIVRFFGFFFLAWQTAELWGNLISSLVLSSGAHGAASVTDEMGNGTGGSHHDDSNLESCGANFCVVETSNNANLQRPPDSEIFEISAIYLSCIVAAVVIIAVFLDPLSRYGERRRGSISATEISGMQLLSATFKQLKKANQQLLIPITVFIGMEQAFIGADFTQAYVSCALGIHQIGYVMICFGVVNAICSIIFGSAMKYIGRVVIIILGAIVHGACIIYLLYWRPHPDHQIVFFILSGLWGIGDAVWQTQINGLYGALFRRNKEAAFSNYRLWESVGFVVAYAYSTNLCARMKLYLLFGVLVCGMVGYTIVEIRQLKKEKHLKKLEEIPKQQQTEADRKVIEEDDEKDELEEDIVVTHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8UZY4</accession>
    <name>A0A6E8UZY4_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">MAP7 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MAP7 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8UZY4"/>
    <dbReference type="EnsemblMetazoa" id="ACON001315-RD">
        <property type="protein sequence ID" value="ACON001315-PD"/>
        <property type="gene ID" value="ACON001315"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000686"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001315"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034867"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015630">
        <property type="term" value="C:microtubule cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051483">
        <property type="entry name" value="MAP7_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008604">
        <property type="entry name" value="MAP7_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15073:SF18">
        <property type="entry name" value="ENSCONSIN, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15073">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05672">
        <property type="entry name" value="MAP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="54"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="106"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="153"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="217"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="309"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="651"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="680"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="781"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="853"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="57"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="71"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="107"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="153"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="219"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="252"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="263"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="315"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="379"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="450"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="680"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="781"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="853"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="870"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="898"/>
            <end position="909"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001315-PD"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="944" mass="104165" checksum="F5FA1B107F39FB60" modified="2020-08-12" version="1">MLTDESDRIPSTTLAVLRGILVKSLSRENLSLTDQQQLLGTRKARHVQFSLAGNRDATVQKQSRPSSANRELQKEREERIKQMKERQNEERQKKLEELKAQALAAQKFREQKEEERRRRMDDLRRRESDRRSQVEERRRAIIEADNERREYILRKNQERDQRMETKRRNDRGSIQFAFGSSTPRMIDTSDSGMCSSFWAHRRATSITNVAYTGAALTRRSSERELTDSASKKRATSASGLDRSTDGLRSGEMTPTSLNTSRPGSAMSSSTTASGIVYRRTLPAQRKPRPASIAVTGVSASGLKEGFATSKHRLSSAGSGSITTTPAKSASGTETPTKKPLTLHSAEKRSAGARGTTPKASSTPLQSPGPEKASRNLLDSLKKSADGPKKAPTPKTPAQKDDAVVVEAKQEPPSVAAPKVEELSAQQEAILQRDDGSQQASLVEVDQSFGQEEQPQQQQEEVVVPVQQEAPAPAQTEDAELPKQAEEAVTVEVVEQAPITKQQQQAPLEQQDVSAVQAAEPTVMVAEEVPENVNGSLTVEVSGAGADAMTASMIAKRINTEEEAKAALAERRRLAREEAERLAELERKRIEAEEQAERQRILEEEERLRKLEEETIRLAEEQRRMEEERLQQAIEEARRRDEEERKRREEEARQKAEREEAERKAREEAERQRVEMAERLKKEEKEREERRKRVEAIMSRTRAKGAANNTPTKNNEDDKENAMSKSQIVLSSTPAALPTGTAMSASTISMTDSFIASEIRQEQQQQQQQTQHQSLEQAMESLSLSNNNTTSSNSSSSSSDTLANSSTINNNINNNNNNTNGGNVQFDKSVTEKENLLLASSTAGAAVVVANNNLNNGSTSNGTVVEPATNEEGTNGTKGESLLLTTNGKSEQQSPAMMASSNGSSNATTANSTSDLIIEDALMGQTNGHKNGTMDNVLFPRVSDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8UZZ3</accession>
    <name>A0A6E8UZZ3_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">furin</fullName>
            <ecNumber evidence="15">3.4.21.75</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Kex2-like endoprotease 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">dKLIP-1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="24">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>Release of mature proteins from their proproteins by cleavage of -Arg-Xaa-Yaa-Arg-|-Zaa- bonds, where Xaa can be any amino acid and Yaa is Arg or Lys. Releases albumin, complement component C3 and von Willebrand factor from their respective precursors.</text>
            <dbReference type="EC" id="3.4.21.75"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase S8 family. Furin subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.75" evidence="15"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8UZZ3"/>
    <dbReference type="EnsemblMetazoa" id="ACON001330-RA">
        <property type="protein sequence ID" value="ACON001330-PA"/>
        <property type="gene ID" value="ACON001330"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009338"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001330"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041309"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097688">
        <property type="term" value="P:glutamate receptor clustering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016486">
        <property type="term" value="P:peptide hormone processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001941">
        <property type="term" value="P:postsynaptic membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097090">
        <property type="term" value="P:presynaptic membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008039">
        <property type="term" value="P:synaptic target recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd04059">
        <property type="entry name" value="Peptidases_S8_Protein_convertases_Kexins_Furin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000001">
        <property type="entry name" value="Furin 2, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000006">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.850:FF:000001">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.850">
        <property type="entry name" value="Peptidase S8, pro-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034182">
        <property type="entry name" value="Kexin/furin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002884">
        <property type="entry name" value="P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032815">
        <property type="entry name" value="S8_pro-domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038466">
        <property type="entry name" value="S8_pro-domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42884:SF3">
        <property type="entry name" value="FURIN-LIKE PROTEASE 1, ISOFORMS 1_1-X_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42884">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01483">
        <property type="entry name" value="P_proprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16470">
        <property type="entry name" value="S8_pro-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51829">
        <property type="entry name" value="P_HOMO_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7 20 21" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 20 21" id="KW-0645">Protease</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 20 21" id="KW-0720">Serine protease</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="P/Homo B" evidence="23">
        <location>
            <begin position="607"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="226"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="22">
        <location>
            <begin position="42"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="22">
        <location>
            <begin position="249"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="19 20">
        <location>
            <position position="317"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="19 20">
        <location>
            <position position="358"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="19 20">
        <location>
            <position position="532"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00035756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00038993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00053600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00076029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00077026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU003355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001330-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="830" mass="91668" checksum="3D33D3F5865A3287" modified="2020-08-12" version="1">MLEQHGGVLRCTSRSDSYFPDERSADQDDPEDPQNELRWHQGSSSSSSDDCSSGSNESSCSAGGCHTRRPRCQSVTVHPRYQQHHLHPRASPWSRIIQFQLVLVLSVVLVFSGINSAVNCDENVTTGVRGSGDGGELKKGLLAGILATANSSSSSSSSSSSDDEAKLLSVNEIDEMLDGVRHFTHHWAVHIPEGDVHGLADRVAAEHGFINRGKIFHEHYHFEHSRLHKRSLSPSKPHQQRLESDRRVRWAKQQRARSRQKRDFRPLTKSYQLPIQLNDPKWPEMWYLNRGNGLDMNVIPAWKEGVTGKGVVVTILDDGLESDHPDLEHNYDPYASYDVNSSDNDPMPHYDLTDSNRHGTRCAGEVAATANNSKCAVGIAYGAKVGGVRMLDGDVTDIVEARSLGHNSQHIDIYSASWGPDDDGKTVDGPGELATRAFIEGVRNGRGGKGSIFIWASGNGGREHDNCNCDGYTNSIWTLSISSTSQEGQVPWFSEMCSSTLATTYSSGNTNEKQVITTDLHHTCTSSHTGTSASAPLAAGIAALVLEANRNLTWRDLQHIVVRTAKPGNLIDPNWSVNGVGRRVSHSFGYGLMDAAAMVRLARTWKTVPEQQVCEINARHLDKQIPPRTKVTLQLIVEHCMGVNYLEHVQAKITLTSQRRGDIKIFLTSPSGTRVTLLTPRSHDLSRSGFNQWPFMSVHTWGEAPHGTWQLEIHNEGRLLAQITQWDLIFYGTETPAQPNDPVRFGKPGYDSNFGGEMEHNALEFDSGVTSDQWRDMQQGHSEVQRTSSNDVTTVSCARFDAASCIGLSLLVMFISQFYSDRQVSSIGSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V1D2</accession>
    <name>A0A6E8V1D2_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ninjurin a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ninjurin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8V1D2"/>
    <dbReference type="EnsemblMetazoa" id="ACON002124-RA">
        <property type="protein sequence ID" value="ACON002124-PA"/>
        <property type="gene ID" value="ACON002124"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012474"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002124"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_036200"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042246">
        <property type="term" value="P:tissue regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007007">
        <property type="entry name" value="Ninjurin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12316:SF17">
        <property type="entry name" value="NINJURIN C, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12316">
        <property type="entry name" value="NINJURIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04923">
        <property type="entry name" value="Ninjurin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="64"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002124-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="148" mass="15976" checksum="75DE034ABAABACC5" modified="2020-08-12" version="1">MAMASILPASSGVGAESDRVLKSMDANRYATKKTIAQGMLDIALLTANASQLKYILQVGEKHEFYGLMLTLISISIILQIVQGIACILLANYNINKEHSRDTANTVQNFSLGLNMATVVVNIMINSMEIKHMDDVGLGMGLESTTKAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V1D3</accession>
    <name>A0A6E8V1D3_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Signal transducer and activator of transcription</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">Ngousso</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="12">Forms a homodimer or a heterodimer with a related family member.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the transcription factor STAT family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040238021.1">
        <property type="nucleotide sequence ID" value="XM_040382087.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8V1D3"/>
    <dbReference type="EnsemblMetazoa" id="ACON000099-RA">
        <property type="protein sequence ID" value="ACON000099-PA"/>
        <property type="gene ID" value="ACON000099"/>
    </dbReference>
    <dbReference type="GeneID" id="120958964"/>
    <dbReference type="CTD" id="42428"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_003675"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000099"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034609"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd09919">
        <property type="entry name" value="SH2_STAT_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16855">
        <property type="entry name" value="STAT5_CCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.532.10:FF:000005">
        <property type="entry name" value="Signal transducer and activator of transcription"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000029">
        <property type="entry name" value="Signal transducer and transcription activator 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.630:FF:000003">
        <property type="entry name" value="Signal transducer and transcription activator 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000048">
        <property type="entry name" value="Signal transducer and transcription activator 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.20">
        <property type="entry name" value="STAT transcription factor, all-alpha domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.630">
        <property type="entry name" value="STAT transcription factor, DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.532.10">
        <property type="entry name" value="STAT transcription factor, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001217">
        <property type="entry name" value="STAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046994">
        <property type="entry name" value="STAT5_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048988">
        <property type="entry name" value="STAT_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036535">
        <property type="entry name" value="STAT_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013800">
        <property type="entry name" value="STAT_TF_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015988">
        <property type="entry name" value="STAT_TF_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013801">
        <property type="entry name" value="STAT_TF_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012345">
        <property type="entry name" value="STAT_TF_DNA-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013799">
        <property type="entry name" value="STAT_TF_prot_interaction"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11801">
        <property type="entry name" value="SIGNAL TRANSDUCER AND ACTIVATOR OF TRANSCRIPTION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01017">
        <property type="entry name" value="STAT_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02864">
        <property type="entry name" value="STAT_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02865">
        <property type="entry name" value="STAT_int"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21354">
        <property type="entry name" value="STAT_linker"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00964">
        <property type="entry name" value="STAT_int"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47655">
        <property type="entry name" value="STAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48092">
        <property type="entry name" value="Transcription factor STAT-4 N-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-0010">Activator</keyword>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 14" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13 14" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="10 14" id="KW-0804">Transcription</keyword>
    <keyword evidence="7 14" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="SH2" evidence="17">
        <location>
            <begin position="578"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="698"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="143"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="698"/>
            <end position="734"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00064301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU046415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000099-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="752" mass="86470" checksum="9A16F8E6D35FD6D8" modified="2020-08-12" version="1">MSLWARVNQLPQPILEQIRFIYGSNFPIEVRHYLADWIEERLLNAPVYTNDQEAVYEQDAANFLNQLIMELERTAINLPESNFTIKIRLNESARNFRQLFSHNPAQLYQHLMNCLHRERQCVAYPDECVNVQDPEVTEVFNAVQQLQIMVRTNENDNRNLMKEYEHLLLEVHELQKNRAQLETIENAEMRAHAHNQLAQHQKMVNDRLQLCTGKRLALVDGFRKTILITDEVQNKVLNKYLSQWKINQGFAGNGASMMSASNLDTIQAWCESLAEIIWSTKDQIRLAIKNKSKLHVEQEDVPDLLPQAMVDVTNLLKMLITNTFIIEKQPPQVMKTNTRFAATVRLLVGNTLNIKMVNPQVKVSIISEAQAQQTQQTNKASEQSCGEIMNNIGNLEYNETTKQLSVSFRNMQLKKIKRAEKKGTECVMDEKFALLFQSSFAVGHGDLVFSVWTISLPVVVIVHGNQEPQSWATITWDNAFADINRIPFQVPDKVIWNQLAEALNMKFRASTGRSLTAENMHFLCEKAFKTNLPFPVPNDLTIMWSQFCKEPIPDRSFTFWDWFYAAMKVTREHLRGPWMDGSIIGFIHKSKAEDYLLKCPRGTFLLRFSDSELGGITIAWVNEGNDGQPQILHIQPFTAKDFSTRSLSDRIRDFDDLFYLYPNKPKHEAFDRYTTPAGPPRNKNYIASEVRAVLMPGPTNNQMNSFPNTPSYNIQSPDASRDTPSTGYGQTNYGQVPDFELENIGMFSSQYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V1X8</accession>
    <name>A0A6E8V1X8_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cytosolic purine 5'-nucleotidase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 5'(3')-deoxyribonucleotidase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040241076.2">
        <property type="nucleotide sequence ID" value="XM_040385142.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8V1X8"/>
    <dbReference type="EnsemblMetazoa" id="ACON000380-RB">
        <property type="protein sequence ID" value="ACON000380-PB"/>
        <property type="gene ID" value="ACON000380"/>
    </dbReference>
    <dbReference type="GeneID" id="120961404"/>
    <dbReference type="CTD" id="32822"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002927"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000380"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030075"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008253">
        <property type="term" value="F:5'-nucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046037">
        <property type="term" value="P:GMP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07522">
        <property type="entry name" value="HAD_cN-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000021">
        <property type="entry name" value="NT5C2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008380">
        <property type="entry name" value="HAD-SF_hydro_IG_5-nucl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02244">
        <property type="entry name" value="HAD-IG-Ncltidse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12103">
        <property type="entry name" value="5'-NUCLEOTIDASE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12103:SF15">
        <property type="entry name" value="CYTOSOLIC PURINE 5'-NUCLEOTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05761">
        <property type="entry name" value="5_nucleotid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="523"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="535"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="546"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="564"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="584"/>
            <end position="598"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000380-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="628" mass="71106" checksum="E3570616EA15A789" modified="2020-08-12" version="1">MYYPTADLPSPQTFPTAGGGGGMAAGEGHVKKYYRKAEHRIFVNRSMHLENIKFYGFDMDYTIAEYKSPQYEQLGFDLVKERLVSLGYPAEIRQFEYDPSFPVRGLWFDSLYGNLLKVDAYGNILVCVHGFEFLKHHQVYELYPNKFLQLDESRVYVLNTLFNLPETYLFACLVDFFTHSPQYAEQVDRTGVKYGELFMSFKSIFQDVRGAVDWVHIYGDLKKKTLENLDEYVKKDERLPMVLSRIRESGAKLFLLTNSDYMFTDRIMTFLFDFPHGGKPDEPHRDWKTYFDTIVVDARKPLFFGEGTILRQVDTSTGALKVGTHMGPLQANQVYSGGSCDVFTKLIGAKGKDVLYIGDHIFGDILKSKKIRGWRTFLIVPELVQELHVWTDKCQLFAQLQQLDVRLGDLYKNLDSSAKEKPDIRSVRTAIRDVTHKMDLAYGMMGSLFRSGSRQTFFAGQVVRYADLYAATILNLMYYPFSYMFRAPAMLLPHESTVAHEQRFTLDAPMIQRTRPPRAELQLSAAAAAPPPHELDDEEEGAEGDADARGDESKEVGRLLLGTPQPSSLPSATGSELSVSVPHTRPETPRSVTHTHDEDYSDEDSDDQKRTKGGEARGAGDGPASAQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V5M1</accession>
    <name>A0A6E8V5M1_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Bromo domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8V5M1"/>
    <dbReference type="EnsemblMetazoa" id="ACON002807-RB">
        <property type="protein sequence ID" value="ACON002807-PB"/>
        <property type="gene ID" value="ACON002807"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009792"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002807"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030253"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05497">
        <property type="entry name" value="Bromo_Brdt_I_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05498">
        <property type="entry name" value="Bromo_Brdt_II_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000003">
        <property type="entry name" value="Bromodomain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.220:FF:000001">
        <property type="entry name" value="bromodomain-containing protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000002">
        <property type="entry name" value="Bromodomain-containing protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043508">
        <property type="entry name" value="Bromo_Brdt_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043509">
        <property type="entry name" value="Bromo_Brdt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050935">
        <property type="entry name" value="Bromo_chromatin_reader"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027353">
        <property type="entry name" value="NET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038336">
        <property type="entry name" value="NET_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22880:SF225">
        <property type="entry name" value="BROMODOMAIN-CONTAINING PROTEIN BET-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22880">
        <property type="entry name" value="FALZ-RELATED BROMODOMAIN-CONTAINING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17035">
        <property type="entry name" value="BET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51525">
        <property type="entry name" value="NET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Bromo" evidence="5">
        <location>
            <begin position="76"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="5">
        <location>
            <begin position="449"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="domain" description="NET" evidence="6">
        <location>
            <begin position="735"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="169"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="355"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="412"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="541"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="604"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="815"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="564"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="604"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="621"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="670"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="688"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="815"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="825"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="874"/>
            <end position="895"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002807-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="901" mass="95662" checksum="0ABDFE4A2883A7CA" modified="2020-08-12" version="1">MQQGSGSSTPSAAPASVVPPAPATVDSMDEPPPRNEPVVEPVNGIVQPPVMPPPERPGRLTNQLHFLLRTVMKAVWKHQFSWPFQQPVDAKKLNLPDYHKIIKQPMDLGTIKKRLENNYYWTSKECIQDFNTMFTNCYVYNKPGEDVVVMAQTLEKLFLTKVSLMPKDETEMEVQQPKGGKKKPRSLAPPGTLVGNTSVTGAAAAAGVAPNAAIVAGANAVAVAAAAAAAARARPGSALGAAVSSSVPPLSAVPPGMGAASTVVAPSVPPIGTMPPQTVPGSTNTTTTAGPNAAVAALTAAAAAVAAVNTPHNAMGNPIGAPVVSKPSAPTNPPYMVNSSQPGMDTVLPPQQPAKVKKGVKRKADTTTPTATVFDPHYGTPMDSNAAKIATRRESGRQDIAPYQTSAYPMSPMAHQGSSSSQYPPKNKEKLSDALKSCNEILKELFSKKHSGYAWPFYKPVDAELLGLHDYHDIIKKPMDLGTVKRKMDNREYKSAPEFAADVRLIFTNCYKYNPPDHDVVAMGRKLQDVFEMRLANIPDEPVNNVAPHHQCKESEPSSSSDTNDSDEESESDEECAQKLKLLEKQLFEMQERMRKLTEEALLKKKQKKKSKDKKKSSVAGPGGAAGGGASDIKGMMDPHAGLSMPHGPGKAMHQMAGVMGQPPMGGAGAAPVMPAAATKAKAKGQRAPKAGGVAGGAASNAPAKRAKNAGAAGGTSAPRAPNKKKASQNVSNFDSEEEDTAKPMSYDEKRQLSLDINKLPGDKLGRVVHIIQSREPSLRDSNPDEIEIDFETLKPSTLRELESYVASCLRKKTRKPYYKKVSGKSKEEQMTEKKQELEKRLQDVTGQLGTGKKNAKKDEANKQDVAPSGGNMSSSSSSSDSSSSSSSDSSSSDSSDSEAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V827</accession>
    <name>A0A6E8V827_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="7">BTB domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8V827"/>
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        <property type="protein sequence ID" value="ACON000080-PD"/>
        <property type="gene ID" value="ACON000080"/>
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    <dbReference type="VEuPathDB" id="VectorBase:ACON000080"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032448"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:animal organ development"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:developmental process involved in reproduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="BTB_POZ_BAB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000138">
        <property type="entry name" value="Fruitless, isoform N"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
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        <property type="entry name" value="Dros_DevTransReg"/>
    </dbReference>
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        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
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        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
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        <property type="entry name" value="BTB DOMAIN TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110:SF107">
        <property type="entry name" value="SEX DETERMINATION PROTEIN FRUITLESS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="5">
        <location>
            <begin position="30"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="6">
        <location>
            <begin position="476"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="112"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="300"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="408"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="604"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="674"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="117"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="146"/>
            <end position="177"/>
        </location>
    </feature>
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        <location>
            <begin position="182"/>
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        </location>
    </feature>
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        <location>
            <begin position="198"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="233"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="244"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="309"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="332"/>
            <end position="343"/>
        </location>
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            <begin position="417"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="674"/>
            <end position="698"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000080-PD"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="711" mass="74389" checksum="DA0E3C6DA0E9030A" modified="2020-08-12" version="1">MDQQYCLRWNNHQSNLTTVLTTLLQDEKLCDVTLACEKGMVKAHQAILSACSPYFEQIFVENKHPHPIIYLRDVEVNEMRALLDFMYQGEVNVGQHNLQNFLKTAESLKVRGLTESSADRYSADTDSKLRSERIRDSRDERDSLPNASSNNSNNNNNSSSNNNNNNNTISSNNNNNSLHHGPLRDKELTEHEQLERLQQQQQQTHHQQQQHPSSHQQQSQQHPSSQHQQPTHQTHHHHHHHQHGGGVGGGGGGGGGGGGGGGSAGPVQQPSRSASIDLMQSALVDERDYLAAEDREISTVENKKKRKMSTTCDNSSPSTPSLMNERQGGYESQASSHSSFKQSPKPEDEFKVSSPAPVHPLAGHPLSGIKQELPELPVRHSLSSELMQPIPINMNADDMNNILAPGNMGSLNESGDSDAHLSHPDHPDNIDGSKAWHMRLTFERLSGGCNLHRCKLCGKVVTHIRNHYHVHFPGRFECPLCRATYTRSDNLRTHCKFKHPMFNPDTRKFENMLSPTMASQAAAAAAAISAAAAAANSSFKPDFSTAAAVANSFKSEQFSSAAAAVANYALGTFKSDFPPIPNSSAAAAAAAVAAAVAASVSPGSGSGGGGGGGGGGSVGSGGIGSSSLGGGGGSGRSSSGGGMIGMHSVAAGAAVAAGGGVAGMMSTGAGVNRGGDGGCGSIGGEVGSVGGGGGGGGSSVRDGNNGGELSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V838</accession>
    <name>A0A6E8V838_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Tyrosine-protein kinase receptor</fullName>
            <ecNumber evidence="16">2.7.10.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="16"/>
    <dbReference type="EnsemblMetazoa" id="ACON000015-RB">
        <property type="protein sequence ID" value="ACON000015-PB"/>
        <property type="gene ID" value="ACON000015"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011876"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000015"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031525"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004714">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032006">
        <property type="term" value="P:regulation of TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000341">
        <property type="entry name" value="Tyrosine-protein kinase receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000112">
        <property type="entry name" value="Tyrosine-protein kinase receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000210">
        <property type="entry name" value="Tyrosine-protein kinase receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002572">
        <property type="entry name" value="Tyrosine-protein kinase receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000643">
        <property type="entry name" value="Tyrosine-protein kinase receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015914">
        <property type="entry name" value="PAPs_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050122">
        <property type="entry name" value="RTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002011">
        <property type="entry name" value="Tyr_kinase_rcpt_2_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416:SF527">
        <property type="entry name" value="PROTO-ONCOGENE TYROSINE-PROTEIN KINASE ROS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16656">
        <property type="entry name" value="Pur_ac_phosph_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63825">
        <property type="entry name" value="YWTD domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00239">
        <property type="entry name" value="RECEPTOR_TYR_KIN_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 16" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12 16" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 16 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1779"/>
            <end position="1804"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="20">
        <location>
            <begin position="463"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="20">
        <location>
            <begin position="954"/>
            <end position="1061"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="20">
        <location>
            <begin position="1319"/>
            <end position="1432"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="20">
        <location>
            <begin position="1443"/>
            <end position="1541"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="20">
        <location>
            <begin position="1542"/>
            <end position="1639"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="1870"/>
            <end position="2140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="2299"/>
            <end position="2339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="2301"/>
            <end position="2313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="2319"/>
            <end position="2329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="2330"/>
            <end position="2339"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="1903"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00051243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000015-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="2339" mass="260545" checksum="99C06F47BA476582" modified="2020-08-12" version="1">MSNTWPPGPTGTGRARNRGGVALRINQWVAGHTSTVNRSLSTVKLLALVATVATVLLSTGVDGSTVPTSAELGEDDYSGAIQDLETGCINRCPDQNQTSFTEHIDASCGNDCYIKQEIPESNTSRHYMFEKLEPERNYSVSVAMSNPAGEGPASVALVATPPKPTGHEEALQPTFILGAERSILAQSSLTLFSDPPTSVYASAEHKIRGTATHIRRGLLFVSDDAGYIYRAPYWPGAEKFRVVILAPGAANNFRPTLLSIDWLNEHLYVLGQARPTKLWQIAYCDFNGGHLTVAIAGLQRRPDHFAVDPYNGYLFWVVGGTGPDGGLFRLDLGDISNGVRHEIRPLQMVRGRSLGAFTLDHANFRVLLADQGLNTVLAVSLDGKKLEDIRNNTQQPRFERVKSLARANGLFYWTNGTEVFAEDYHRLHDSYYHNAFPIAANNTYFSISVNLTAEQPIPVPVNPPRNLQALASPDKLKVSWDVPYLLGVKGRGAWQAWSYRVEVSDEQLGSVLHVATVNGTSYACDTAGRLLPNRVYSVRAAAFTAAGRGPWSREFRVRTLRPASRHHLVWASTDGIVRSDVIGEHVATLIPRSPELDGAAVTSLAWHARTLYVVSNSTLRLYREPAVPGGEWAKVRELESVECVAIDWIGERLYYSNPAQQLIVRAGLQGEQPEPIHSVMNVREIRFDAYRGFIYCSSGLILEAFRLNGKSRVLYFSEKRFTDKQIIGLTLDHDGERLYWIVRGYSHSHLYWAPLAGTAAARSASGSGSLPSLPLADQKPLGSLVHFSDRLLWLKESQVVVGDARGENLAYIRNHHLNGTRALALIDPMPATPSINVLPASVNASSIRVTGDWRRFNVSWTPVENVNYSGVFYKLTLKLPDARDIVQELTVPYFVYGGGAGDETENEGVSAASAEQWPIPPYTPIDITVHAFTYWRSSGFSTVRRHTPAGKPSVPVAPRAYIQHHYLPGTHELSTGIVFRWSPPVEPNGPIVAYRVDCWSTGQDDVRRTVLDGEEVQTGHTELLIPGAVQPNMTYYLQVRVCNVDHSGDPSEMRSIRLADGRPLPLLYVATQDHILLLDLDRRESTPIVATAVPALLLAELRHERKLLWVNLNGELFMYDEADGKRKIAQVPSRATALTVDWVARAVYVRSQTPSGNGSTLHAYDLNRFESGGSDPVHRMVSLPAEPAVIDLLQVRPGVAELVAVSRYARAGYIVSLDEPDREPTPFNCSGEDVENEWNQRLCGEYDGVFDVDGQHGIVQDEGYLYWIGPDDGTVSVRARDGAPTSDATTQQFPLTGAVVLLPVQHRQAYPPERCLVPVVQQIQYLPELYNNTESSITLRLPEPERHTDCERRPPAVRYRILYQQEDEASADLHIDYSYESSRTIGNLRPFTNYRFGVVLTSYYLAAATTAQRLLVGGEPSITELPKRSMPVVFRTAVGPPSRPEDITAWPVSPTEAIVSWIPSRQKNSHHVWYEIWRAEPDEHKNRQQQVVTDFKMDDTSMTVEITRLQPNQTYIVWVRAYSNFHAYSDSDKFQIQTFPEPSDIQLLSLNSTGIRLRWIPPVNCQKYKIQYAIVGQSTWTTMYDSQGPSTHWYNYHYELNGLMPKTRYQFVVLLHYPYRDEPYVWPRERDIIFETAADKPAAPGRPIVTKLRQDVYKVSWEAAKDNGAIIEEYALEVLVSQLNRAARFVLSPDDDTAAGELAPGVEGSNGTATVGELETYDERWSQVYNGTDVYWIIPERHAIQRNLFRVRARNSYGWGPYSDESRPTGPELYVQRTIIYIATFVSTLGTIVVVVALTVIFLIRQTDKMKNFQMDPANARLSDVELANLRELPRRGNFVQTTNILYSSGSMTDSEIALLPRIRLDQIFMASSSLLGSGAFGEVYEGVVKGVDGEAETRVAIKTLKKGAKLHEKQEFLQEAQLMSNFKHKHITRLLGVCLEADALLIIMELMQGGDLLSYLRRSRSLPGQAARLTMLDLISMCQDVASGCRYLEEMHFVHRDLACRNCLVSSTDPRDRVVKIGDFGLARDIYKNDYYRKEGEGLLPVRWMSPESLVDGVFTSQSDIWAFGVLLWEIMTLGEQPYQAKNNVEVLNHVREGGHLDRPKVCPNEMYELMKYCWKFSPEERPTFRYCLEVLRALRENTSEDTQIIAPFPAKLQQEAVTNPAYLVSESGQVSPLSGSLRYSGSDAGLIATPPTSSSGSSGATACPGPNTPKYLELVYEDSGDLDQPDGFVASVDRVSPIPPMPTDNGYEIPITDSRGQIQFAEIPTTSHPPQTTSQRQASVPVLLPNLSVLLPDSASTGANRDSQQVEPPSLCAATASTTSSSIIEREERQTRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V8E3</accession>
    <name>A0A6E8V8E3_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Discs large MAGUK scaffold protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the MAGUK family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8V8E3"/>
    <dbReference type="EnsemblMetazoa" id="ACON000255-RD">
        <property type="protein sequence ID" value="ACON000255-PD"/>
        <property type="gene ID" value="ACON000255"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012236"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000255"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030881"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043296">
        <property type="term" value="C:apical junction complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031594">
        <property type="term" value="C:neuromuscular junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098839">
        <property type="term" value="C:postsynaptic density membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043297">
        <property type="term" value="P:apical junction assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009792">
        <property type="term" value="P:embryo development ending in birth or egg hatching"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045197">
        <property type="term" value="P:establishment or maintenance of epithelial cell apical/basal polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043113">
        <property type="term" value="P:receptor clustering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097120">
        <property type="term" value="P:receptor localization to synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099072">
        <property type="term" value="P:regulation of postsynaptic membrane neurotransmitter receptor levels"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00071">
        <property type="entry name" value="GMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06723">
        <property type="entry name" value="PDZ1_Dlg1-2-4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06724">
        <property type="entry name" value="PDZ2_Dlg1-2-4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06795">
        <property type="entry name" value="PDZ3_Dlg1-2-4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11861">
        <property type="entry name" value="SH3_DLG-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001402">
        <property type="entry name" value="Discs, large homolog 3 (Drosophila)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000001">
        <property type="entry name" value="Disks large homolog 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.63.10:FF:000001">
        <property type="entry name" value="Disks large homolog 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000058">
        <property type="entry name" value="Disks large homolog 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000048">
        <property type="entry name" value="disks large homolog 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000004">
        <property type="entry name" value="Disks large homolog 4 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.63.10">
        <property type="entry name" value="Guanylate Kinase phosphate binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016313">
        <property type="entry name" value="DLG1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008144">
        <property type="entry name" value="Guanylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020590">
        <property type="entry name" value="Guanylate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050614">
        <property type="entry name" value="Synaptic_Scaffolding_LAP-MAGUK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23119">
        <property type="entry name" value="DISCS LARGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23119:SF51">
        <property type="entry name" value="DISKS LARGE 1 TUMOR SUPPRESSOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001741">
        <property type="entry name" value="MAGUK_DLGH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00072">
        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00856">
        <property type="entry name" value="GUANYLATE_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50052">
        <property type="entry name" value="GUANYLATE_KINASE_2"/>
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    </dbReference>
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        <property type="entry name" value="PDZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0965">Cell junction</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 12" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="PDZ" evidence="16">
        <location>
            <begin position="55"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="16">
        <location>
            <begin position="152"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="16">
        <location>
            <begin position="343"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="14">
        <location>
            <begin position="454"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate kinase-like" evidence="15">
        <location>
            <begin position="630"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="518"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="566"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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            <dbReference type="ARBA" id="ARBA00022949"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
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            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="PROSITE" id="PS50002"/>
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    </evidence>
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            <dbReference type="PROSITE" id="PS50052"/>
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            <dbReference type="PROSITE" id="PS50106"/>
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        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
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    </evidence>
    <evidence type="ECO:0008006" key="19">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="820" mass="89614" checksum="800EC574C4D2DF33" modified="2023-06-28" version="2">MTTRKKKRQQDGGGGAGGGGFLKKVSSLFSLDTVPTSNANDTKQLNGDSPWEYDEITLIRGSTGLGFSIAGGTDNPHINLDASIYITKVIPGGAAHVDGRLQVNDCIVAVNEVRVVNVTHGEAVDALKQAGDRVTLHIRRKRPPAQAPKLEEIELLKGSKGLGFSIAGGIGNQHIPGDNGIYVTKIMEGGAAHIDGRLAVGDKLIAVRTPDGGERNLENVVHEEAVATLKAITNRATLIVQKTNILQALANSISNLNSLSTNAMNSVGVSMVDHASPDAGGGGGEAGGGDTEMVASTARSHSPAFGLENSSSRYASSNVLETGMPIGTPRAVSSEDITRVPRTIVIRKGASGLGFNIVGGEDGQGIFVSYVLAGGAADLGGELKRGDQLLSVNGISLANASHEDAAQALKNAGGTVTLVVQYRPEDYNRFEQRIQELKQAVAAGTGTLLRTTQKRTLYVRALFDYDPNRDDGLPTRGLQFRHGDILHVTNASDDEWWQARRVIGEDEEESIGIVPSKRRWERKQRARDRSVKFQGHAGANNNNNNLEKVSTLDRKKKNFSFSRRFPFMKSKDDKNEDGSDQEPNGNLSDPNHHDPDQPFMLCYTQEDANAEAEENILSYEPVQRLQISYTRPVIILGPLKDRINDDLISEYPNKFGSCVPHTTRPKRDYEVDGRDYHFIASREQMEKDIQNHLFIEAGQYNDNLYGTSVASVREVAEKGKHCILDVSGNAIKRLQVAQLYPIAIFIKPMSVESIMEMNRRMTEEQAKKTYERTLKMEHEFGEYFTAIVQGDTIEEIYSKVKNVIWSQGGPTIWVPSKESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V8E4</accession>
    <name>A0A6E8V8E4_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ATP synthase F1 subunit epsilon</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ATPase epsilon family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8V8E4"/>
    <dbReference type="EnsemblMetazoa" id="ACON000260-RB">
        <property type="protein sequence ID" value="ACON000260-PB"/>
        <property type="gene ID" value="ACON000260"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001180"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000260"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041617"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042776">
        <property type="term" value="P:proton motive force-driven mitochondrial ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12153">
        <property type="entry name" value="F1-ATPase_epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1620.20:FF:000005">
        <property type="entry name" value="Uncharacterized protein, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1620.20">
        <property type="entry name" value="ATP synthase, F1 complex, epsilon subunit superfamily, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006721">
        <property type="entry name" value="ATP_synth_F1_esu_mt"/>
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    <dbReference type="InterPro" id="IPR036742">
        <property type="entry name" value="ATP_synth_F1_esu_sf_mt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12448">
        <property type="entry name" value="ATP SYNTHASE EPSILON CHAIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12448:SF0">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT EPSILON, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04627">
        <property type="entry name" value="ATP-synt_Eps"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48690">
        <property type="entry name" value="Epsilon subunit of mitochondrial F1F0-ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000260-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="56" mass="6510" checksum="882006F3B44F5D3C" modified="2023-06-28" version="2">MAAWRAAGLNYINYSNIAARLLRKALKPELRAQAVRRDDSHIKFTKWQNGKPENEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V8E8</accession>
    <name>A0A6E8V8E8_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Cytoplasmic dynein intermediate chain</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynein intermediate chain family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040220755.1">
        <property type="nucleotide sequence ID" value="XM_040364821.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8V8E8"/>
    <dbReference type="EnsemblMetazoa" id="ACON000320-RA">
        <property type="protein sequence ID" value="ACON000320-PA"/>
        <property type="gene ID" value="ACON000320"/>
    </dbReference>
    <dbReference type="GeneID" id="120948099"/>
    <dbReference type="CTD" id="44160"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_008273"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000320"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041504"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005868">
        <property type="term" value="C:cytoplasmic dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045504">
        <property type="term" value="F:dynein heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045503">
        <property type="term" value="F:dynein light chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010970">
        <property type="term" value="P:transport along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000781">
        <property type="entry name" value="Cytoplasmic dynein intermediate chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025956">
        <property type="entry name" value="DYNC1I1/DYNC1I2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050687">
        <property type="entry name" value="Dynein_IC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442:SF22">
        <property type="entry name" value="CYTOPLASMIC DYNEIN 1 INTERMEDIATE CHAIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442">
        <property type="entry name" value="DYNEIN INTERMEDIATE CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11540">
        <property type="entry name" value="Dynein_IC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 7" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="472"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="72"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="628"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="16"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="79"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="642"/>
            <end position="657"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000320-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
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</entry>
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            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
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                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
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            <strain>Mali-NIH</strain>
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            <strain evidence="3">Ngousso</strain>
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</entry>
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    <accession>A0A6E8VA91</accession>
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                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
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        <source>
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        </source>
    </evidence>
    <sequence length="1821" mass="192560" checksum="5037BBF350970188" modified="2020-08-12" version="1">MEEELSDGDIVWVKVSNSWWPGEVMGSSRLTEEFLSSLRKKPLAVVKFFQEDSYEYVKNPNFIFKYNCPRKHEFLRKGLEQYRAKMKHMEKFPADVMHAERATGGDPDIVNSTDYLPQKKERHSGAFQDGRSPAEKPNTPAKLNTPPFSFGSGRKTIHEVRILAQPSSTPSEGEAAPARKASSEKMDESAEEVDPEPDIKPDRARRSLPVADKAKPLETPIRNVAVPAAVKSEPPSSEKRTRGGRKTTPTVQAPKQKKGRQNKPDQSSSVLDEGKQASAKVSEHEDTRASANVDVTVEEEKEENHRQNREKGSGSVSHEESPTQHAVKNKTDVKLKNELLADWSEDEQEDDEGDKLAGGVENEEEKNPTKESRKDTPPKDVSPVRTTKASTRKQQKDTKQETKQQSLASKKTAKPEAKNETVAMANKDAESVAVKETEKDPESPPESSTVVAATAPPVTAATAGSVTAAVVSTSSASSAAPIVSPHATIKYRNIPKKQKREFIEVTNDDVSMVQEKYAVPSREGSTASSSSTTGSNESCAGPSQSIVPPTAPAAERPISAKQLILNRATRGSSKSLSGDEITAASAPVPADHTTPSVSTDTNAVQEERQSHIDDLAKKTDVQQQETSCFDFNDDEDVHQQEKVSPASQTSLMTARFDRKRDQAAAAREKSTTVEPEPSEVDRDAQLSQEIDSLLGETNVPKLPEDLSDVMTAVSDKIDCNRTLPPKERGKRIFKTRNKTVTASSSSSPKRDDARVGEEVVPSEGSVPAEAANNNGDSSSSAGDVAKEKTEMEQQPQQQEASEEETETVKGKDESKNREDDQNPSVTSSAMTDEVLPEDKELQQKQQHQSAKKKRKSDEVLLPDSSGNGDQPTVPAATRRRGKYSKAVTDVHEKSVGEKSVSTQSVSEAREPSSVSRKAKRLRKEGPELDEATPANTDDSDKSTAEVAKVTKRDVSSEIKTSVAMDTAEATDNSNDLSAVEKQEATDGALAKHLKTNHEESVATQQDAAATTLLTSKATPTDLQVAEALINLPVVVTAPLPVSSSSTSSSSAVDEDVSSKAKVVAVEPTKEADTTASSSTQAVSVSVECAGQPTPNNTSSSSNSNNNSSTAKSINPRKRHLQSMMLTTAEATNLPQSKNVAAVVEPPMTTSGIEKEITTVKVVLPPEEDPPSIPEKKKRNEVLTDGEEMASKESSPVSAEVEKLDINSMPIVMDEGELLVDGTSSIPAPAGAITVMKSVNGAGGGAAKPPLVAATSKDKRQTTVAMVKGGKASTEQIVITSKGTVLTTSTASVSVASKFPSSTKSSVTVTSAITLSSSCRVASSTPAPSVSSPPSSASSAAAPQLVPPKIIITKKPLPHAASTSTASSKAGSAEGSVGSKAHDRRVSGDGASSSVSSSSGSETITGKKSHRVLRLTPQKLKEFSRLGYIEERQGKGKMLTKSGMRQLYGKQQQLQQQQQQQQQQQQQQQHVSRKKPVPISTKIITTTGGGGADHAVAPDSNSSSVRPTSLPEVASSGSNVVIAAAADSSVQESSTTVGRTASTDPSAVDMEEDEEMLEQQKVSSGEAGELPASTHVAPAGGAAILLEGSPSSSGSGAAEVILGSSELAVVGGGSVTEASSSSTGGSGSGTTATGEQESSQLIAVPAENFGGPPHLFYLCSVRDESLVPVNNELLYLDASNQLVALPEQHQQHPSQQQQQHQGQQQQQQQAAGTLTEEIINQAEVIIPTVGGSNGAEMGGDGTGEGGTTAIANATGAVGSDGAQEGFVFNTQDGQHIILDPQSLMALAASGDTPHLITADGQEIILQGSLFTSSVSVDDPDYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VA94</accession>
    <name>A0A6E8VA94_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="9">BZIP domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VA94"/>
    <dbReference type="EnsemblMetazoa" id="ACON001464-RB">
        <property type="protein sequence ID" value="ACON001464-PB"/>
        <property type="gene ID" value="ACON001464"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011211"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001464"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040022"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14689">
        <property type="entry name" value="bZIP_CREB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051381">
        <property type="entry name" value="CREB_ATF_subfamily"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45996">
        <property type="entry name" value="AGAP001464-PB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45996:SF3">
        <property type="entry name" value="CREB-H TRANSCRIPTION FACTOR HOMOLOG LET-607"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="8">
        <location>
            <begin position="598"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="139"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="520"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="935"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="616"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="86"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="139"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="164"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="178"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="196"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="536"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="950"/>
            <end position="959"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001464-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="966" mass="104583" checksum="129A40415B7E3F19" modified="2020-08-12" version="1">MSLAEDNDYMQDEWPEEDFMNSLLKMDDTMLFSEPPATHTGGADELDDFMRQLNYSYTADTYLGEQQVTSPLSSHASTDQEFHGFPSTTDGGSSSASLDGYFDEGYGVRMQDKPELMNVDFSMLNVLDPGSPIEQEHISSAASDSGLSSDHLDLDPSTEYEALSPGMSSPGPSISERGGQNSPPRFTISVKQEPSVPTTPTATAQQPPVTVQQVQPVVPRQQPAVLAQPMAATQRTAQTTVTTAVPVRSAEKLISTKTAIVTNGAPTVLKTSANKTATTTVYQQPGTGMKSLIANGTKRLIASTNGISASTVPQQLVGSNGKELKIIRMATMKNGQTVAVANGLTTAGTTGTNKKVTLQLKNSTLKAGVGGTGGSTIIPKNVVLTTSSSGTNGHSSAASTTTTTTTTVPGVRKIIRMQQQTQQNGRQILVPVTIQDLRTIKIVNSTNMKGRSSANIKLAAANMLQQSKQGLIQKNVVLSKDQLLLDDGVLSDAGGPSSSSDSESYVFEDIMQQSSAVLAEVERQQQKHHSSIISDSDVEPDGDDGDDDDVDMEEIGKGRNQNGTYQKLMLTAEEKRLLAKEGISLPTSYPLTKHEERELKRIRRKIRNKISAQDSRKRKKEYVDGLEERVKQCTEENQNLVKRIKILQSQNHDLVSQMKRIQSLLTKGTSKTTQPATCLMVLLISMALVAVPNLKLGNTATQQNIQDSIEMSELMQDPANDKLQVVQQSRRALLFDTKEQSHAAATAEEELNFDEIMSSFNANALIANEHDYFSELDGGRSGGGEPPSAKRSKLLLPTTTTLIDYDVDDDVWNGGSKIRIKQEAAGSMSPDSSSNGNGNNELFEQKVREFQASLQKSIRDAAEEHTTDMMMAAKYDLNSEVIVDRDLFDLVGKQQQQQQDGGLYEMSLDTFSKAGLNNGGIDLLGSVRAEPTVVSADSTGGDASDARKLNASQQIQQQNNKRKLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VBG5</accession>
    <name>A0A6E8VBG5_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Guanylate kinase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MAGUK family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VBG5"/>
    <dbReference type="EnsemblMetazoa" id="ACON002711-RE">
        <property type="protein sequence ID" value="ACON002711-PE"/>
        <property type="gene ID" value="ACON002711"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000007"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002711"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032683"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005911">
        <property type="term" value="C:cell-cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00071">
        <property type="entry name" value="GMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06798">
        <property type="entry name" value="PDZ_MPP5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12036">
        <property type="entry name" value="SH3_MPP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.63.10:FF:000002">
        <property type="entry name" value="Guanylate kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000088">
        <property type="entry name" value="MAGUK p55 subfamily member 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000469">
        <property type="entry name" value="MAGUK p55 subfamily member 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008144">
        <property type="entry name" value="Guanylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020590">
        <property type="entry name" value="Guanylate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014775">
        <property type="entry name" value="L27_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004172">
        <property type="entry name" value="L27_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050716">
        <property type="entry name" value="MAGUK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035601">
        <property type="entry name" value="MPP5_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23122">
        <property type="entry name" value="MEMBRANE-ASSOCIATED GUANYLATE KINASE MAGUK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02828">
        <property type="entry name" value="L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00072">
        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00856">
        <property type="entry name" value="GUANYLATE_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50052">
        <property type="entry name" value="GUANYLATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51022">
        <property type="entry name" value="L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="L27" evidence="9">
        <location>
            <begin position="612"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="8">
        <location>
            <begin position="757"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="6">
        <location>
            <begin position="849"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate kinase-like" evidence="7">
        <location>
            <begin position="999"/>
            <end position="1179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="158"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="280"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="394"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="175"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="206"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="280"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="290"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="331"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="394"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="432"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002711-PE"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1195" mass="130813" checksum="DA2B3F9AA9B50F5F" modified="2020-08-12" version="1">MQQRTTTPAAGPPPPTASSSGKQVVELNGYVIILVESRDGKIKLYGSPADKDNLEVADEILDVNERKLEDSPRAEVIKHIHECIQSCMIKLRVKRRSDSRLAGELGNAVQDAFVIAVEQQARERLQRLSALKRITPVDMSQLSIKARPHREMAVDVPESFIARNKTPPRYPPPRSTVTAASAAASTPATPASTQVNHVSNAMPQVAKHHHHHNHHHQQQQHPNHHLHHHHPPSSVHHNTHHHHGGGGVSLELEPLDSYSERHLSSLDSSSQEYVKKGIISGGGVGGGGSSKAPSSLGSVTSEFEPSLNGFGPLHGKHHHHSTVGGGGGDLLRGSLSTNRSPSPSSSGDLGPPEYDVGPHRELPVDVPDSFVEMVKGPPRYPPPKPLIIKDAIRKKESCSSSESIDKPKPQTQSPSPPARNELNGSSKPVPPPRDHLRIEKDGRLTNRAPVPAPQVPDRKIVPNASSQHQHIGQVLEPTPDQLDSIKKFQEQLRRRREDEERIAAQNDFLRNSLRGSQRLRALQDNPIEKPPVGVDNEAYADDEVVAVAAEKIIGYGELVAALQRLQGHLNKHGLAALAGRVTAAQSLLLGPGIARALAVRTAVLERRRPKIPNPICPNAQALAKDCVESLAQSRSQTAIELCDLLSTYEMEGLLLAHDRIASTTDRSPIASYGGSPITVPVPLGTASNNTTSNNTTTSNNSINNNTLTSAVKPATVMPSIPNNASLVNNNNNNIAKREPMSVPLGVLRDGSQDHIKIIQIEKSSEPLGATIRNEGEAVVIGRVVRGGAAEKSGLLHEGDEILEVNGIEMRGKSVNDVCALLGSMTGTLTFLVVPAGVPQILPGLGMRDPPVLHVRAHFDYDPEDDLYIPCRELGISFQKGDVLHVISRDDPNWWQAYREGEEDQTLAGLIPSQSFQHQRESMKLAIAGEVGLRTRKDINGKAGGGSTLLCARKGRKKKKKASSEHGYPLYATATADDPDPEEILTYEEVALYYPRASHKRPIVLIGPPNIGRHELRQRLMADSERFAAAIPHTSRAQREGEVPGQDYHFISRQQFESDILARKFVEHGEYEKAYYGTSLEAIRAVVASGKICVLNLHPQSLKLLRSSDLKPYTVLVAPPSLEKLRQKRIRTGEPYKEEELKEIIATARDMEARWGHLFDMIIINNDTERAYHQLLAEINSLEREPQWVPSSWLHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VBY1</accession>
    <name>A0A6E8VBY1_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">FXNA-like protease</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">Ngousso</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M28 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VBY1"/>
    <dbReference type="EnsemblMetazoa" id="ACON003078-RA">
        <property type="protein sequence ID" value="ACON003078-PA"/>
        <property type="gene ID" value="ACON003078"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_008953"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003078"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035711"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008235">
        <property type="term" value="F:metalloexopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03875">
        <property type="entry name" value="M28_Fxna_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000008">
        <property type="entry name" value="Endoplasmic reticulum metallopeptidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053973">
        <property type="entry name" value="ERMP1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053974">
        <property type="entry name" value="ERMP1_1-A_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048024">
        <property type="entry name" value="Fxna-like_M28_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045175">
        <property type="entry name" value="M28_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12147:SF22">
        <property type="entry name" value="ENDOPLASMIC RETICULUM METALLOPEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12147">
        <property type="entry name" value="METALLOPEPTIDASE M28 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22249">
        <property type="entry name" value="ERMP1-TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22248">
        <property type="entry name" value="ERMP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="36"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="390"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="428"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="464"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="505"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="528"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="556"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="597"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="627"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M28" evidence="16">
        <location>
            <begin position="155"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="Endoplasmic reticulum metallopeptidase 1/1-A TM" evidence="18">
        <location>
            <begin position="427"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="domain" description="Endoplasmic reticulum metallopeptidase 1-like C-terminal" evidence="17">
        <location>
            <begin position="659"/>
            <end position="830"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00078796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF04389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF22248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF22249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003078-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="831" mass="92110" checksum="FCE2C96DBFB4806D" modified="2020-08-12" version="1">MFQPTGQSSKQKHPKVKVLDPDIDYSKAKSVHSISSWWGIGGIFIVLVVGNITNYTNSHLPDGLRNAHLTHFPAAFIAERAWKDLKILNDFGPKPTGTYTNEVLAVDFLNREISYIDQLKNRNQQLVVQNQIVSGGYVGVYMNKSAANVYRNVQNVVVKLVGRSESTSRHALLLNCHFDSVAGSPGASDDSGSCAVMLEVLRVLSRQSDVNRYSIIFLFNGAEETPLQASHGFITKHPWAADVRAFINLESAGSGGKEMLFQSGPKHPWLIEAYARAVPHPYAQAAAEEIFQSGVIPSDTDFRVFRDVGRIPGMDFAHTANGYRYHTRYDSIDYIPLPVLQRTGDNILALTRAIANGDELGSTERYAQGYMVFYDFLGLFFVSYSADVGLMINLSVVLLSIIIPFLSLARSTSGTHGRQIRSETMTGFLATFLGAGASGVLCFFIGLQLDAIGRSMSWYSSTNLILGVYCCPALLCQCVVHLLCNRLFGSKTTPLSLALKVQARLNGVNLFWGMITLGITFTGYRLAYIFMVLILCSLCSSTLISMLGLQNTVHKWLLLHMLFQIVALAWSTQFYHILMNMFVPITGRIGSSINPDVIVGTLATFATLFSCSYLTPLLFLLKKTDKLIGELVAITLIALVLATSTHVGFPYRDDAVKAPAVQRHYITHTVRKFYDHNGGERYTDAGFLLQELDRNAKKTIEGIAMPDVVTPMREIPACDKELFCAIPFYSIWHQVLNYWLPGPAPVVRQAVTVSLREKEQLSEHEHRLHLVLKGSFQCSLIIGPKAGSTLKRWSLLSEIPTPIEFNGQRGHFVLLTAGVESEPMNITLDVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VBZ3</accession>
    <name>A0A6E8VBZ3_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Transcriptional adapter</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_040226688.1">
        <property type="nucleotide sequence ID" value="XM_040370754.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VBZ3"/>
    <dbReference type="EnsemblMetazoa" id="ACON003109-RB">
        <property type="protein sequence ID" value="ACON003109-PB"/>
        <property type="gene ID" value="ACON003109"/>
    </dbReference>
    <dbReference type="GeneID" id="120951813"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_013453"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003109"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040670"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:SAGA-type complex"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02335">
        <property type="entry name" value="ZZ_ADA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000170">
        <property type="entry name" value="Transcriptional adapter"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="3.30.60.90">
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041983">
        <property type="entry name" value="ADA2-like_ZZ"/>
    </dbReference>
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        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055141">
        <property type="entry name" value="TADA2A_B-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000433">
        <property type="entry name" value="Znf_ZZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043145">
        <property type="entry name" value="Znf_ZZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12374:SF63">
        <property type="entry name" value="TRANSCRIPTIONAL ADAPTER 2-BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12374">
        <property type="entry name" value="TRANSCRIPTIONAL ADAPTOR 2 ADA2 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22941">
        <property type="entry name" value="TADA2A-like_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25299">
        <property type="entry name" value="ZZ_ADA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00291">
        <property type="entry name" value="ZnF_ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01357">
        <property type="entry name" value="ZF_ZZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50135">
        <property type="entry name" value="ZF_ZZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZZ-type" evidence="11">
        <location>
            <begin position="6"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="12">
        <location>
            <begin position="65"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="10">
        <location>
            <begin position="67"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="362"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="383"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003109-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="421" mass="48668" checksum="DC552BC4B6C92813" modified="2020-08-12" version="1">MSTELFAKYTCTNCQEDISGIRVHCVVCTDFELCLACFAAGAEIGPHRNDHSYQFMDSGILSIFRGKSGWSAREELHLLDAIEQYGFGNWEDISKHIETRTPEEAKEEYVSKFLNGTIGRHTWQTAVDQRPILTDHTSDDTGPLSQLLIQKLPPMDCTAEEAAALGYMPNRDDFEREYDPTAEQLVSTLSLQPDDEDVDMLLKLAQVDIYTRRLRERARRKRVVRDYQLIGNFFRGNMKRARQTRDQREFRERLRTYSQFYTSLEFERLISSLERERALRIRLSELNRYRWNGIQRVDECVHFEQHVAAAQYRNTGPYGHGRTLACIIGPNGQSITGCIRQILTGQPKQRRLWRERQRGILGVGQRNGPNGGGGPADCDTRGIGKKKRRRPRLKFHRPKAHAPHRRPGLLRRLIQQQKLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VFN8</accession>
    <name>A0A6E8VFN8_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_040221903.1">
        <property type="nucleotide sequence ID" value="XM_040365969.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040221904.1">
        <property type="nucleotide sequence ID" value="XM_040365970.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VFN8"/>
    <dbReference type="EnsemblMetazoa" id="ACON003206-RB">
        <property type="protein sequence ID" value="ACON003206-PB"/>
        <property type="gene ID" value="ACON003206"/>
    </dbReference>
    <dbReference type="GeneID" id="120949027"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010338"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003206"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037727"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008028">
        <property type="term" value="F:monocarboxylic acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17352">
        <property type="entry name" value="MFS_MCT_SLC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000271">
        <property type="entry name" value="Monocarboxylate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000372">
        <property type="entry name" value="Monocarboxylate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF286">
        <property type="entry name" value="GH22266P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="51"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="91"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="120"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="146"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="178"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="210"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="549"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="584"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="614"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="638"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="676"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="704"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="317"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="769"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="322"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="361"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="771"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="782"/>
            <end position="791"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003206-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="818" mass="88038" checksum="7864A454ED05CA4A" modified="2020-08-12" version="1">MASKKARRTDSEIACEEAARLTADTQEYTSPDDDDGGCEYHDMPPPPDGGYGWVIVFASFMCNMIVDGIAYTFGVFLNEFVVYFGEGKGTVAWVGSLLSGMYLSAGPVVSALANKYGCRAVCIAGSIISCAAFALSTLSTSVTMLMLTYGVMGGIGFGLIYLPAVVAVGYYFETKRSLATGIAVCGSGFGTFAFAPLANMLLENFDWKNSNLILAGLILNCAVFGAMMRPLTYPKEDKVKPLMQRMYEEKRLQMERGSIGGSYFMVQLPDGTMEKRLKAPLNADPGVHSSLALDQLAAQQGGMHPVATLPTISEFKAQEQNGSSGSSSESSQIEMKKPLNKKRNTNSESDAADYGADNMPRNASQPAFTSHQSGMPKNGSVPTFDRVRKHSTGERFKPSLAAIKASSRGDVGSNGDVRKSMHLRLSRGSVNGSKNNNAEEFDDGSMFTSKASLKADRPAMVRPLSRKDIFYSGSVTNLKEFQSQKSLTNYRNSVVSLTKFEKEHRNDVRDDVEKGREEQYDLCPCLALPESFKNAIGAMMDVSLLRDPVFMMIGVSNIFGMAGLYVPFVYLVDAAVLDGIEQNSASFLISIIGITNTVGRIVCGYVADFPQVDALFLNNICLVISTVAVALTPFCHSYAAYVAMAIAFGIAIAGYISLTSIILVDLLGLDKLTNAFGLLILFRGAATIVGSPLAGALYDATQSYSIPFFVAGGLFALSAITSFAAPAMKRFRKPSEAPVHVEVLTPIDEEPSEDLADDDQPITMVPKIIQTAPSPSTEQPVTSNITSTTTINDDRKQPQSNGSAKDTDKEVSQMESVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VGB2</accession>
    <name>A0A6E8VGB2_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="8">BTB domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VGB2"/>
    <dbReference type="EnsemblMetazoa" id="ACON003439-RQ">
        <property type="protein sequence ID" value="ACON003439-PQ"/>
        <property type="gene ID" value="ACON003439"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005866"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003439"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034718"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18315">
        <property type="entry name" value="BTB_POZ_BAB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000036">
        <property type="entry name" value="Mod(Mdg4), isoform H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051095">
        <property type="entry name" value="Dros_DevTransReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007588">
        <property type="entry name" value="Znf_FLYWCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110">
        <property type="entry name" value="BTB DOMAIN TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110:SF92">
        <property type="entry name" value="MODIFIER OF MDG4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04500">
        <property type="entry name" value="FLYWCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="7">
        <location>
            <begin position="32"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="118"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="192"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="287"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="192"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="222"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="294"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003439-PQ"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="497" mass="55057" checksum="E55DE87EF5A4B9F6" modified="2020-08-12" version="1">MADDEQFSLCWNNFNSNLSAGFHESLQRGDLVDVTLAAEGHLVKAHRLILSVCSPYFRKMFTQVPVNQHAFIFLKDVSHSALQDLIQFMYCGEVNVKQDALPAFISTAEALQIKGLTETGDSAPTHQSPAKEEPAAAAAVPVTTATISTATIPASPASQRAKVQRNRIQSYKLESEESGDDKVVHIQATTSHHVSAQSNLSSQKRTMPQRGLQSHASKRTKMSISASSDGLDTSDSTPAQVQTVQTVQIVKQIPAQVIEPEYIELPIESINPKAEPDYTDETAEIETVDAETEQEQKLSEHDQGDADDDGNYVEDDTYGDMAMGKYEESYLTEGEEGAKPGVSGFVDSYTSDGGNATEISTQATHTSPKAHLFIRSQRGLPLLVKNKFIYRCERTRNHRSYWLCTRYKTHKCTGRIICQNNTVLKETEHCHMDDSRRLHHSELKLVDMSQINIECWVKSVPPRFSSSARKSTSSISSSRIKAEKPDQELGCLLTGKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VGB9</accession>
    <name>A0A6E8VGB9_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="8">PH domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the OSBP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VGB9"/>
    <dbReference type="EnsemblMetazoa" id="ACON003484-RA">
        <property type="protein sequence ID" value="ACON003484-PA"/>
        <property type="gene ID" value="ACON003484"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006622"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003484"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040252"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015485">
        <property type="term" value="F:cholesterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13286">
        <property type="entry name" value="PH_OPR5_ORP8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.2720:FF:000002">
        <property type="entry name" value="Oxysterol-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000030">
        <property type="entry name" value="Oxysterol-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.120:FF:000004">
        <property type="entry name" value="Oxysterol-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2720">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037239">
        <property type="entry name" value="OSBP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000648">
        <property type="entry name" value="Oxysterol-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972:SF102">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01237">
        <property type="entry name" value="Oxysterol_BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144000">
        <property type="entry name" value="Oxysterol-binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="4" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="778"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="7">
        <location>
            <begin position="63"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="674"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="805"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="678"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="697"/>
            <end position="709"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003484-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="825" mass="93364" checksum="86F2D405E96E9FF4" modified="2020-08-12" version="1">MKFFLGITGWNDNSPKIESGFPDKNDSAKLNRKESYKAQRKNYRREKKRVASELLNSLQDPAVIVLADWLKIRGTLKSWTKLWCVLKPGLLFIYKSPKTKSSHWVGTVLLTSCQVIERPSKKDGFCFKLFHPLDQTIWAPRGPDKETMGAVVQPLPTSYLIFRAPSQAAGKCWMDALELSLRCSALLVRSVPNIDTNLSEGATSTTHETQWSEADYEKHFDEHDLDIDSQLDQGTMVSAGESDSESDLGEGMDVEEFPETPYVHSPEEEFGAAGEQVEELPEEHKSLIWFLVKQVRPGMDLSKVVLPTFILEPRSFLDKLADSYYHADILSKAVLEDDPFTRMKTVVQWYLSGFYKKPKGLKKPYNPILGETFRCYWQHENGSRTFYIAEQVSHHPPVSVFYVTNRQDGFCISCSILAKSKFYGNSTSAILEGVATLTLLPRGESYTLAVPYAHCKGILMGTLSMELGGKVSIECENTGYKTELEFKLKPFLGGNEYTNLVVGKLKLGKETMATINGHWDSEIRIKDSRTNEETVLFNPTPDIRNRRLKRFTVPMESQGMNESERLWQHVSAAIHRDDQVGATEEKTALEEAQRISAKERKATCTEWVPLLFQQDFVTGQWHYKFADLRPWDPRNDLRQYESNYRIQTKTRHQAPMVRTASVVSTDPLNILQVDSRGSLRKNRSGVMKSRKISSNTSTPDLNDPNSDSSHSNDHDTVLSSTRIMQTLDDINKKLEEHSKKLVKLQLDMDKIHWAPPRRQQINADGPLAFLSSINVKTLMLIVVGLLVQLLLSWFLIKRSLTTVTTSDTREGGDGDGGESAGNNSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VGC2</accession>
    <name>A0A6E8VGC2_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="12">MFS domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">Ngousso</strain>
        </source>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
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    <dbReference type="EnsemblMetazoa" id="ACON003492-RA">
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        <property type="gene ID" value="ACON003492"/>
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    <dbReference type="VEuPathDB" id="VectorBase:ACON003492"/>
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        <property type="component" value="Unplaced"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="130"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="165"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="196"/>
            <end position="216"/>
        </location>
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        <location>
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        </location>
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        </location>
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        <location>
            <begin position="297"/>
            <end position="315"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="321"/>
        </location>
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            <begin position="1"/>
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        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
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    </evidence>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
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            <dbReference type="ARBA" id="ARBA00023180"/>
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        <source>
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        </source>
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            <dbReference type="EnsemblMetazoa" id="ACON003492-PB"/>
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    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="336" mass="36091" checksum="B94EF816C8F64659" modified="2020-08-12" version="1">MSSSAPGATNPMLYDPIPEGQGSTTKRNQFVAALGICMAAVSGGTALAWTSPVLAQLVPANQSDTSGLEHESFLLTTDEGRRIDAGVALKWFWGPNADTQSALQTIQSDLDAASGEAKVSDLFTNPTNRAALFISLLLMFFQQFSGINAVIFYTAPIFQSAGSTMDPAVCSIVVGVVQVVMTLASSVLIDKAGRRILLLQSSFIMGACLIVLGVYFKMQNDKVDVSNIGWLPLASVVLFIISFSLGFGPIPWMMMGELCAPDIKGLASALAVMFNWTLVFLVTKSFGTMQELLGSDWTFWFFGAWMMVCTVYVFIKVPETKGKTNAQIQAILGGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VGE3</accession>
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        <recommendedName>
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        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
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            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
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                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <strain evidence="8">Ngousso</strain>
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        <text evidence="6">Positively regulates a late step in synaptic vesicle exocytosis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the complexin/synaphin family.</text>
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    <dbReference type="AlphaFoldDB" id="A0A6E8VGE3"/>
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        <property type="gene ID" value="ACON003509"/>
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    </dbReference>
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    </dbReference>
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            <begin position="21"/>
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        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="132" mass="15388" checksum="0F5009035CAB98D8" modified="2020-08-12" version="1">MNFLGAAGGAVGGDGGDDENDKEKEEEAERERQEAIREAEERRKEKHRKMEEEREKMRQEIRDKYNIKKKEEIVEQAPQEEPNPLMRKKKTPEELAAEAEQEDLDDFTKLKNSIETQVNEIKTQIEGKCSLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VHR2</accession>
    <name>A0A6E8VHR2_ANOCL</name>
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        <submittedName>
            <fullName evidence="1">C2H2-type domain-containing protein</fullName>
        </submittedName>
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        <name type="common">African malaria mosquito</name>
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            <taxon>Diptera</taxon>
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            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VHR2"/>
    <dbReference type="EnsemblMetazoa" id="ACON003773-RB">
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        <property type="gene ID" value="ACON003773"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002277"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003773"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032477"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003773-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="179" mass="21353" checksum="A86C102B861A123A" modified="2020-08-12" version="1">MIATVKQFHDDFDRIVLHKDIYRTFYYYYLQEIRFRIIDILMNFAIIEWCEPKPAIVATDPYGFPMPEPAKKSKRRKDDEPAEIPDYTIRVKCYKEKFPLVCCDDQFRTLAALRLHYYAVHDKTYLLPATRCEMKAAMLRMLVFRGQLDEWIASGIASNNGLCFYLTKLLRRIISIIRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VI42</accession>
    <name>A0A6E8VI42_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Ig-like domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VI42"/>
    <dbReference type="EnsemblMetazoa" id="ACON004108-RA">
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        <property type="gene ID" value="ACON004108"/>
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        <property type="entry name" value="CLUMA_CG000981, isoform A"/>
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        <property type="entry name" value="Neural/epithelial_adhesion"/>
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        <property type="entry name" value="CTX-RELATED TYPE I TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="LACHESIN"/>
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        <property type="entry name" value="Ig_3"/>
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        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
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        <property type="entry name" value="IGc2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026078573" evidence="9">
        <location>
            <begin position="25"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="32"/>
            <end position="133"/>
        </location>
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        <location>
            <begin position="144"/>
            <end position="229"/>
        </location>
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        <location>
            <begin position="236"/>
            <end position="327"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
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            <dbReference type="ARBA" id="ARBA00022475"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
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        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004108-PA"/>
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    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="337" mass="37437" checksum="8DFDF06A87A5757E" modified="2020-08-12" version="1">MWLLRMNLLLIITLLSVALTLSNAQQASTASPSIVFISPEQIKDIGEQVTLNCSIANVKNYIVGWQKSNRDRTKESNIISLGVQLAVTEDRFRLNFTKENNAANYVLEIHDIVNTDAGLYECQIQVNSTSKITKTVELQVRHPPMLLENQHTNTLTKAEGEDAQLVCRAEGYPRPTISWRREYNAILPIGGQTFTGNELRLNGLRREDRGTYYCTADNGVGRADTKTITLEVEFAPVIRVPRPKVAQALDYDIDVECVVQAFPAPAISWYRDGKQIHNGGSYSISQIGSPDDVTTSVVKIHSVAAEHYGDYVCKATNKVGQAEARLNLFESVIPVTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VIF9</accession>
    <name>A0A6E8VIF9_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Coiled-coil domain-containing protein 128</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VIF9"/>
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        <property type="gene ID" value="ACON004226"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011476"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004226"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031678"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040024">
        <property type="entry name" value="PPP1R21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049372">
        <property type="entry name" value="PPP1R21_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019343">
        <property type="entry name" value="PPP1R21_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21448:SF0">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21448">
        <property type="entry name" value="SMOOTH MUSCLE MYOSIN HEAVY CHAIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="PPP1R21_C"/>
        <property type="match status" value="1"/>
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    <dbReference type="SMART" id="SM01254">
        <property type="entry name" value="KLRAQ"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein phosphatase 1 regulatory subunit 21 N-terminal" evidence="3">
        <location>
            <begin position="13"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="198"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="24"/>
            <end position="72"/>
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            <begin position="111"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="323"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004226-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="387" mass="44232" checksum="B9AE0ABFCACD092A" modified="2020-08-12" version="1">MEAMEAENSNKYQKLATEYSKNKNSSLRESLRLKEATLRKSEQEVDSLGFRNKQLERRVAALQENLEHELKKVSGGKTLKTKSSNNDLNAVAGTETTGVIAEELQKKILENAQLSNSVEDKSAEVKQLQNRIESLNRELQQQATVEQKLRKELELLTVRNSELESKVSEAASTIGSEDGLSVTTDVENHFNHFTASSNSASQVQNNGTVNTTNNNNNNTTTGTQDDRVVFLEKELNHWRAQYELLKIETSSHIRIAPKVAEQKEDIFSHGADGINRTLEDNQQQNEVQTREQKLTECFTKSIEELFHDKCRAESKLASHFIECQRLQTHLDVLKSRLKDREEANREEERRYRIIEDELSRTRLNYEEQISVLTEQVISLSDQLAKAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VIG2</accession>
    <name>A0A6E8VIG2_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pre-mRNA-splicing factor ISY1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ISY1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VIG2"/>
    <dbReference type="EnsemblMetazoa" id="ACON004225-RB">
        <property type="protein sequence ID" value="ACON004225-PB"/>
        <property type="gene ID" value="ACON004225"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002886"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004225"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_029913"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000350">
        <property type="term" value="P:generation of catalytic spliceosome for second transesterification step"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.660:FF:000001">
        <property type="entry name" value="pre-mRNA-splicing factor ISY1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.660">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029012">
        <property type="entry name" value="Helix_hairpin_bin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009360">
        <property type="entry name" value="Isy1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037200">
        <property type="entry name" value="Isy1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13021">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR ISY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06246">
        <property type="entry name" value="Isy1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140102">
        <property type="entry name" value="ISY1 domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="186"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="214"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004225-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="240" mass="27593" checksum="7008DA6709AFDECA" modified="2020-08-12" version="1">ARNAEKAMTTLARWRAAKETETGKQERRPYLASECRDLGQCEKWRLEIIREISKKVAQIQNAGLGEFRIRDLNDEINKLLREKRHWENQISDLGGPHYRRYGPRMFDAEGREVPGNRGYKYFGAAKDLPGVRELFEQEPPPPPKKTRAELMKDIDADYYGYRDDDDGILLPLEEKAEKLAIQRAVEEWNSAKGGSKTAEKDDDEPDIYGSDAYITRDPEQQTKEDPMGLLGPRFTAHVPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VII1</accession>
    <name>A0A6E8VII1_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="14">GATA-binding factor 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
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            <taxon>Anopheles</taxon>
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    </organism>
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        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
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    <reference evidence="14" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">Ngousso</strain>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
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        </location>
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        <location>
            <begin position="113"/>
            <end position="125"/>
        </location>
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        <location>
            <begin position="200"/>
            <end position="215"/>
        </location>
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            <begin position="333"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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</entry>
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        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
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            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
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                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
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                <consortium name="EnsemblMetazoa"/>
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        <text evidence="1">Belongs to the recoverin family.</text>
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    </dbReference>
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    </dbReference>
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        </source>
    </evidence>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="326" mass="36478" checksum="445476503F115309" modified="2020-08-12" version="1">MAGPHLEESTVTTPSITGGVVVNTASLSLSRPTNLESSICTIDDFLAHFKHKELENDVSKYLSHKQRPLCRRVLAYLRTAWMGAKFTSKSGIYLMAWRSLGCCVVVRLEEIVSPPKYRPESIAALCEATRFTEAEIKRIYRGFKAECPAGIIREETFKFIYSQFFPQGANTGLYAHYVFNTLDKEHTGILSFENFVQGLSILSRGTLEEKLCWTFSLYDINHDGKITREEMTDIVTAIYELMGARDDGGTDEVNIKNKVDTIFQKMDTNQDGVITIEEFLDCCRKDQLISSSMGVEVDADELPGSQEGPKDDGSNENSNESSLQTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VJV2</accession>
    <name>A0A6E8VJV2_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Potassium channel domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VJV2"/>
    <dbReference type="EnsemblMetazoa" id="ACON004717-RC">
        <property type="protein sequence ID" value="ACON004717-PC"/>
        <property type="gene ID" value="ACON004717"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005457"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004717"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030172"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015271">
        <property type="term" value="F:outward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022841">
        <property type="term" value="F:potassium ion leak channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030322">
        <property type="term" value="P:stabilization of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003280">
        <property type="entry name" value="2pore_dom_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11003:SF334">
        <property type="entry name" value="FI03418P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11003">
        <property type="entry name" value="POTASSIUM CHANNEL, SUBFAMILY K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="36"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="142"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="337"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="363"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="397"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="10">
        <location>
            <begin position="345"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="170"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="219"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="270"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="170"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="230"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF07885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004717-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="444" mass="50571" checksum="49320FF5BE7F8A2F" modified="2020-08-12" version="1">MSSRRTAPPPMQQLQPQMSISYGEPEKKPRSKCMSFVCCMWKVFTCIFSHVTLVATVVAYCFLGAFTFEHLEAENERNVKKGISSIRVNLTDAIWKMTNDRPVLHQQNWTSAAIMHLQSFEKEILTAMKKDGWDGNEDVDQIQWTFFGALFYSIIVITTISVQAAGTNLRRSVRNSQRSADSGFDPYYEPGLSHSYSDTDYMDDDSPCKTPILCNKYALDDVDDQDGRSTRPNPRSQSMPRQTGRYGDRLMPDRMPYYTEDNEIAENMQSVRKSAPQRRREPRPVPAPSPRIMSPMGFAVHRQARHLQNVIDDNSLYDDEWDLHSGELPPSSIVRPVPIWLCVFLVVSYIIAGAFMFSEWEEWSFLDSAYFCFITLTTIGFGDFVPAQGVKNDSEISIALCSLYLLFGIALLAMSFNLVQEEVISNVKSVARRLGILKEEELDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VJV6</accession>
    <name>A0A6E8VJV6_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ras-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="EnsemblMetazoa" id="ACON004692-RD">
        <property type="protein sequence ID" value="ACON004692-PD"/>
        <property type="gene ID" value="ACON004692"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000481"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007480"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004692"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038199"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04013">
        <property type="entry name" value="C2_SynGAP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05136">
        <property type="entry name" value="RasGAP_DAB2IP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.506.10">
        <property type="entry name" value="GTPase Activation - p120gap, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021887">
        <property type="entry name" value="DAB2P_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039360">
        <property type="entry name" value="Ras_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023152">
        <property type="entry name" value="RasGAP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001936">
        <property type="entry name" value="RasGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057606">
        <property type="entry name" value="SynGAP1-like_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10194:SF60">
        <property type="entry name" value="RAS GTPASE-ACTIVATING PROTEIN RASKOL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10194">
        <property type="entry name" value="RAS GTPASE-ACTIVATING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12004">
        <property type="entry name" value="DAB2P_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25321">
        <property type="entry name" value="PH_RASGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00616">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00323">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00509">
        <property type="entry name" value="RAS_GTPASE_ACTIV_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50018">
        <property type="entry name" value="RAS_GTPASE_ACTIV_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="21"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="5">
        <location>
            <begin position="423"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GAP" evidence="6">
        <location>
            <begin position="606"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1273"/>
            <end position="1297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1436"/>
            <end position="1463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1558"/>
            <end position="1614"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1717"/>
            <end position="1739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1273"/>
            <end position="1284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1285"/>
            <end position="1296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1574"/>
            <end position="1595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1719"/>
            <end position="1730"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004692-PD"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1739" mass="195610" checksum="EE097E2FC405269A" modified="2020-08-12" version="1">MFYQTTNYQYLKICRDRDCLYILRVVHCIPCFVWSFIDQLRIIHDSSLLMIEQENKIKRYGVVLNASVNIDDDETKCHEEQTSCRLQSNTSTPIRLRKSRSRSNILSNLSKDHGIAVENLASRSGIVCDLIRREQSNTMSDNSILTAKDKSKTLPQNLFLVNSMPPKSSFLHHSSSRIGNNSTFDSKKSSSIAGLSSKLPAPAMDYMISPTKSISFIRRTHSTKLSRSNSLLKSLTSKCVDQSGCNTSLLRIPVKELEYDRLCRILEKPAQLDHMIRDIFILCKDEKVEENAVHSDTSYEKACRRGSAPSTPVMGQKGESTSRFTNFFSKRSFRSIPLKRTKSVIKLERSKRGQGGIRGSRSHESLLSTHVVMSNIDLACIGTIEVVPVHSSVLGRRHCFQVRGISHEERYYSCGTRQERDIWIHSLRKSISPNKDNRRRLDNSLKMWVYEAKSLPPKKRYFCEIYIDKTLYGRTSVKLRADLLFWGEYFDFPDIPEASIITVNVYREAEKKKKKDKHVLVGSVVIPIEKVAARTFSEDWYQILMEKQDNVIKSPSKEPAPTLRIKCRYQSIDIMPLDVYGEFHEFLKKNYKKICEVIEPIIGVKAKEDIGQALVLQMHSQGMAAIFLSDVVALDLLRVDDQRLTFRGNSLATKSMEAFLKLIGEQYLQDTLSIPIAEIIASDRDCEVDPTKVNGSLSRQQQALRRTVKSVWIAIAESSKNFPKQLRDCFATFRERLHDLDREDMADNLISASIFLRFLCPAIMSPSLFNITNELPSARATRNLTLVAKTLQTLANFTRFQGKENFMEFLNDFLEQEAPRMKQFLYDISCSNFSNPDNFMDWSGCIDQGKQLSILHSLLYEIVLKLPADKQHEIQPLPAILATITHCKQVNCTTLNADQYCTGKPHIVQVKENLLSKKEDILQSAGSANMSSKEYSTKRNPKILKNECTVQSSNTLQPSTHSSSFSLHYSTSTSTNSLVNNTKQLDKDATDKNDDICAMGKLNFNSQYGSNIICSGSLRTYSSTGATSFGAVNSLMTNSLRNEKEKSNNLQGDIRANTLPRYNNYTTSVSIRENTYPESSNSVGYDHEVNGNSRLNGINKNLIQIDIDPTNPINRKSPTPLLKNISCCHYIANNRIQEGSHQRLTSPGSNLSDLDKNAFNLGIPHNDTNREQQYSQHIYPAPALKASNITTISSFVPRQSTTTFNPSKMPMSLEDLEDLLNYADEQNQTSFSESVSNRGGCDPCGTNTKANIASNGSNASISQISNICSSGYQSIPTQSTSSSPVESTGEQHSLVTNRAPPSRKLTTTLIGYNIGTSQKLCMNDQAIAKADLPVSSINMQLSNKSDPLVTSHGTIHHYYDRKTPNEQSNVNRILNNNYVDISYDVLSPCKSPIASKKTECTSLGIDEHNDLAHPVEYFGINRTNHHSRHKRKVIHGIRRSGSSIQSESSDDERVSNTTSENFNDIDSLTTGNVSALERNHILISNGNDYDQASSGLFGCRLGCRRMPRTNPLMQYDKDDVSDIIGNSGVDTALTNHQRNQSFQSRFHRRLSIECARALSDSSTDETESEKTKTFNSNQHTAQHHNLSISTTTHTNIFHENDTRRRRNANKSVEQCEREIQRLQASLDSMRKKLEMSEPAEGNANKLAAITQQSDSKIRSIIGRLLTMEEELRQEQYKMSLVLSHKQRVIEAQGKQIAALDAANNRLLSALSTLQNRYESQSNQSDDTSSSHPNAGASSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VJW2</accession>
    <name>A0A6E8VJW2_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">DNA replication ATP-dependent helicase/nuclease</fullName>
            <ecNumber evidence="22">3.1.-.-</ecNumber>
            <ecNumber evidence="22">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="27 28">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="27">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="22">Key enzyme involved in DNA replication and DNA repair. Involved in Okazaki fragments processing by cleaving long flaps that escape FEN1: flaps that are longer than 27 nucleotides are coated by replication protein A complex (RPA), leading to recruit DNA2 which cleaves the flap until it is too short to bind RPA and becomes a substrate for FEN1. Also involved in 5'-end resection of DNA during double-strand break (DSB) repair by mediating the cleavage of 5'-ssDNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="22">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="22">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 22">Belongs to the DNA2/NAM7 helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="22"/>
    <dbReference type="EC" id="3.6.4.12" evidence="22"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8VJW2"/>
    <dbReference type="EnsemblMetazoa" id="ACON004685-RB">
        <property type="protein sequence ID" value="ACON004685-PB"/>
        <property type="gene ID" value="ACON004685"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007562"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011014"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004685"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039499"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017108">
        <property type="term" value="F:5'-flap endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017116">
        <property type="term" value="F:single-stranded DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033567">
        <property type="term" value="P:DNA replication, Okazaki fragment processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071932">
        <property type="term" value="P:replication fork reversal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18041">
        <property type="entry name" value="DEXXQc_DNA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22318">
        <property type="entry name" value="DNA2_N-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001170">
        <property type="entry name" value="DNA replication helicase Dna2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026851">
        <property type="entry name" value="Dna2/JHS1_DEXXQ-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045055">
        <property type="entry name" value="DNA2/NAM7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048459">
        <property type="entry name" value="DNA2_Rift"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014808">
        <property type="entry name" value="DNA_replication_fac_Dna2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011604">
        <property type="entry name" value="PDDEXK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047187">
        <property type="entry name" value="SF1_C_Upf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887:SF433">
        <property type="entry name" value="DNA REPLICATION ATP-DEPENDENT HELICASE_NUCLEASE DNA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08696">
        <property type="entry name" value="Dna2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21123">
        <property type="entry name" value="Dna2_Rift"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 22" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="13 22" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="22" id="KW-0158">Chromosome</keyword>
    <keyword evidence="10 22" id="KW-0227">DNA damage</keyword>
    <keyword evidence="18 22" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5 22" id="KW-0235">DNA replication</keyword>
    <keyword evidence="16 22" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="12 22" id="KW-0347">Helicase</keyword>
    <keyword evidence="11 22" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14 22" id="KW-0408">Iron</keyword>
    <keyword evidence="15 22" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7 22" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="20 22" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="6 22" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8 22" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19 22" id="KW-0539">Nucleus</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA replication factor Dna2 N-terminal" evidence="23">
        <location>
            <begin position="2"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2 rift barrel" evidence="26">
        <location>
            <begin position="309"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase helicase" evidence="24">
        <location>
            <begin position="461"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase helicase" evidence="24">
        <location>
            <begin position="565"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase-like C-terminal" evidence="25">
        <location>
            <begin position="642"/>
            <end position="906"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00047995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="RuleBase" id="RU367041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF08696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF13086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="Pfam" id="PF13087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="Pfam" id="PF21123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004685-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="951" mass="107786" checksum="518AFC9B305F16A9" modified="2020-08-12" version="1">MSESENTQMVIGTVVHYIFQRCLLDKSCKLLTHVETIAKKVMKTKKVISSLYEVNLSTKEAFSLLGPYLKEIETFLNKHFNHRSIQTDTETIAICEVNDIEENIWCHHLGVKGRIDATVSVSSDATKKTFEIMPLELKTGRASYSFEHLGQLALYQMMMNLVGHEVNAGLLLYLKEGKCSRVTANRNMKRDLIILRNEVARSLSKWMVKDNITQKGSLAMKPTLPDPINNERACTKCPYNTVCITLLKSEREGTVTNYGLSILAEEACGHLRTKDVDYFIQWSGLIYLETHDETVQSRNVQNMWNSSPKERAETGRCIYGLILVSPVRIVDDLYFHTFKLEAASSTLPKALDTFQVGEYIICSTSKRIAVASGYIISHASNEIVVSFERDLSTNYGAETFILDQNTLYKSTFNLSNLALLLFNDDRCSQYRRIIIDREKPTFSDGFLSKSMIPKAKEILKKLNRHQKNAALKAAATKSYCLLKGLPGTGKTQTIVGLIRLLSLLGQSILLTSNTHSAVDNVLKRLLPFQDLKFIRLGSIDRIDPAVASSAEAIVTEHCDSPEKLSEVYEQYKIVGVTCQGTGHPLINKRLFDFCIVDEATQVFQPSLIRPLLRSKRFLLVGDPEQLPPVIKSVEARSLGACESMFHRLDQEGSFYILPTQYRMNRVLTKLANEFAYNGKLICGNDIVENNTINLPNLENIRRIYEVERWLLKMISNQIDLSVVLVDTGNTYQMNLNYRKLNEISTSITDDQSKNEMNCTNVSEIAIVVYVSWAFLQAGVEPESIGIIAPFRAQVELIRKLMKKLFEKQKYTRHSSSSNHNVIYTKENVEQLNHTCNIEVNTIDQFQGKDKKIILFSCTKSSNLSDDIWINKGKERSSHGYEILSDKSRLTVAITRAKEKLIIIGDRLTLDSYAPFKKLFNVASKISNISLREKKDGFEWNALLEFLISLSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VJW3</accession>
    <name>A0A6E8VJW3_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Homeobox protein abdominal-A homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Antp homeobox family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VJW3"/>
    <dbReference type="EnsemblMetazoa" id="ACON004662-RC">
        <property type="protein sequence ID" value="ACON004662-PC"/>
        <property type="gene ID" value="ACON004662"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_013719"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004662"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031157"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009952">
        <property type="term" value="P:anterior/posterior pattern specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000317">
        <property type="entry name" value="homeobox protein abdominal-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022132">
        <property type="entry name" value="Abdominal-A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050296">
        <property type="entry name" value="Antp_homeobox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45659:SF4">
        <property type="entry name" value="HOMEOBOX PROTEIN ABDOMINAL-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45659">
        <property type="entry name" value="HOMEOBOX PROTEIN HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12407">
        <property type="entry name" value="Abdominal-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5 9 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 9 10" id="KW-0371">Homeobox</keyword>
    <keyword evidence="7 9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="12">
        <location>
            <begin position="135"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="9">
        <location>
            <begin position="137"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="206"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="206"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="222"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="247"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00072555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004662-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="307" mass="33050" checksum="0C55EEC1FB4334A4" modified="2020-08-12" version="1">MYPFVSNHPTTHTSYSTMPGFSGLDDKSCSRYTDSVMNSYPPMGVPGSASIAQFYQQAAAVSAASAGVGVDSLGSACSQLSSSVGGAQSGLPDITRHPWLVTASQSALQKFASTDWMSNPFDRVVCGDFAGPNGCPRRRGRQTYTRFQTLELEKEFHFNHYLTRRRRIEIAHALCLTERQIKIWFQNRRMKLKKELRAVKEINEQARREREEQDKMKNESLKSAQQHHSQKQAQQEHTVVGSQQTSNGGGTGGGTGGSGGAGSGGSSGNLGSHLHHPSIVSQNDLKLGLGGMGVGVGGNLSMMGAQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VJW7</accession>
    <name>A0A6E8VJW7_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">PSI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VJW7"/>
    <dbReference type="EnsemblMetazoa" id="ACON004689-RC">
        <property type="protein sequence ID" value="ACON004689-PC"/>
        <property type="gene ID" value="ACON004689"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_003150"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004689"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038039"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002165">
        <property type="entry name" value="Plexin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031152">
        <property type="entry name" value="PLXDC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13055:SF12">
        <property type="entry name" value="LD40707P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13055">
        <property type="entry name" value="TUMOR ENDOTHELIAL MARKER 7 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01437">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026021754" description="PSI domain-containing protein" evidence="9">
        <location>
            <begin position="27"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="507"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="47"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004689-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="564" mass="63836" checksum="B47157270F9C5B42" modified="2020-08-12" version="1">MMRMMLIIRGSISWLICLSLLQLCVCVKVPRKYLQSDPAEIGISSVVPLASGNGNGNTNESGTGDMPFNRSILPLDENKKRQKKVFPNDEESANKSVFPSPTKLGAVGLNTSLIADSSTRDPISDAINIDTIERTETELNSTLQEHNITKTFEDNHLYYKSTWSTDKTMSEEFWKKISTNLTVNMLLSNSHRRATTMILSFDFPFYGFPIRNVTIASGGFLYTGDYVHSWLASTQYIAPLMANFDTALSNNSFVKYRDDGETFTVVWENVILQDRPSNGTFTFSVTLNKSGDIVFAYKKIPISIQHIFENPLMKNHPVKIGLSDAYIIDKTIMRTKQKTIYEYHRVNFGQTEVTNDTIITLTSLPTCFSFKDCESCITHEGADFECLWCPTMNRCSTGTDRKRQDWVHKGCEATTISEILYCPALGQKGNNYGESMDVTSKITDRYNSTSSIRDNYQPAQDDKLHQNTNGVAQNGSYIHNGSKKEEFVNKAHLYHTTDMDSGSSNKLIVSLLVIILIMLICGCWVLYAYRNPHTKSGQLLIRYRPNKWLWRRGEARYTAASIHM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VK73</accession>
    <name>A0A6E8VK73_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Scavenger receptor class B member 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Plays an olfactory role that is not restricted to pheromone sensitivity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CD36 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VK73"/>
    <dbReference type="EnsemblMetazoa" id="ACON004845-RB">
        <property type="protein sequence ID" value="ACON004845-PB"/>
        <property type="gene ID" value="ACON004845"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011398"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004845"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033958"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005044">
        <property type="term" value="F:scavenger receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002159">
        <property type="entry name" value="CD36_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11923:SF50">
        <property type="entry name" value="GH19047P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11923">
        <property type="entry name" value="SCAVENGER RECEPTOR CLASS B TYPE-1 SR-B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01130">
        <property type="entry name" value="CD36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01609">
        <property type="entry name" value="CD36FAMILY"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="55"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="480"/>
            <end position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004845-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="507" mass="57066" checksum="E25500287AAC3930" modified="2020-08-12" version="1">MPEMVAFYSPETVIPTTSDTEPRKPLKRRTSALNTIFYYLGLEQRSGEDAKENQWIRILTFVVLFVTFITSAIGFFIMWFTNMYNNSLLEVHVFNYTNTEDFFAGKATKLQVEDLGPYVYKETANKVDITHNGDGTISYREHRYIQYLPEESKGKPFDQVVVPNVVFLTGVSKKRSEGTWNQIAFNMAASSSGSSAFIKKPVESMLWGYEDKLLSLAKSMFGSDIVSSSFGMLMTRNGTSAENFTIFSGESSLEDLAVIKHLDGKSRLDLWHTDECDRVGGTDGSQFPPHLMDRKHPLQVFIKSLCRKFPLVYDSEVTALDGIPAWRYKIPNNVFSHPDEHMPNHCFCHLESGSCPPSGLFNITGCSMGAPIFASFPHFYTGDRKLIESIEGVNPVQEKHETYADIHPRLAFPIDGASRFQINIQVQKAAMVSGLEKFTEGQYLPVIWLEVVPGVISDDLRAMIYHSTYSANAIQMSLRVGSLAFFVLSVVLLIAKCFYSTRSTSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VK82</accession>
    <name>A0A6E8VK82_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="13">RRM domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus speckle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the splicing factor SR family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040230502.1">
        <property type="nucleotide sequence ID" value="XM_040374568.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VK82"/>
    <dbReference type="EnsemblMetazoa" id="ACON004592-RB">
        <property type="protein sequence ID" value="ACON004592-PB"/>
        <property type="gene ID" value="ACON004592"/>
    </dbReference>
    <dbReference type="GeneID" id="120954026"/>
    <dbReference type="CTD" id="41670"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033712"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12337">
        <property type="entry name" value="RRM1_SRSF4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12600">
        <property type="entry name" value="RRM2_SRSF4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000028">
        <property type="entry name" value="Putative serine/arginine-rich splicing factor 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000420">
        <property type="entry name" value="serine-arginine protein 55 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047190">
        <property type="entry name" value="RRM2_SRSF4/6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050374">
        <property type="entry name" value="RRT5_SRSF_SR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23003">
        <property type="entry name" value="RNA RECOGNITION MOTIF RRM DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23003:SF51">
        <property type="entry name" value="SERINE-ARGININE PROTEIN 55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="7 10" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="4"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="112"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="74"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="179"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="82"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="202"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="264"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="274"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="295"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="310"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004592-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="345" mass="40131" checksum="E7715E681D8C657B" modified="2023-06-28" version="1">MVGSRVYVGGLPYGVRERDLERFFKGYGRTRDILIKNGYGFVEFEDYRDADDAVYELNGKELLGERVVVEPARGTARGPSGYRERDRYDRDRRGGRYDKNSSRYGPPLRTEYRLVVENLSTRVSWQDLKDYMRQAGEVTYADAHKQRKNEGVVEFATLSDMKTAIEKLDDTELNGRRIRLVEDRGRNGRNGRGRSRSSSSRSRSRSRRRSRSRSRSRRSSRSRSKSRSRSKSKNGSKSPEKSRSRSRSVKSRDNSKSKSRSRSPKRDRDESRSRSRSRSKSHSRSRSKSKDNKSRSRSRSRSRSPRSHSRSRDRSQSHDKSHDKSRSASPKNNDHSPERNESMED</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VK89</accession>
    <name>A0A6E8VK89_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Receptor expression-enhancing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the DP1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040223737.1">
        <property type="nucleotide sequence ID" value="XM_040367803.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VK89"/>
    <dbReference type="EnsemblMetazoa" id="ACON004819-RA">
        <property type="protein sequence ID" value="ACON004819-PA"/>
        <property type="gene ID" value="ACON004819"/>
    </dbReference>
    <dbReference type="GeneID" id="120950064"/>
    <dbReference type="CTD" id="36569"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010070"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004819"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035204"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004345">
        <property type="entry name" value="TB2_DP1_HVA22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12300">
        <property type="entry name" value="HVA22-LIKE PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12300:SF161">
        <property type="entry name" value="RECEPTOR EXPRESSION-ENHANCING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03134">
        <property type="entry name" value="TB2_DP1_HVA22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="69"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004819-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="159" mass="18022" checksum="05382FAEEB02F261" modified="2020-08-12" version="1">MPRQDGAEAGAPKDNSGYIALGGVAVVILYLAFGYGAQILCNVIGVAYPAYISMKAIETRTKEDDTKWLTYWVTFGVLSVFEHFSFFLVQIIPFYWLLKCLFHIWCMVPMENNGSTIMYHKVIQPYFKKYEKVADSFISDTTDKLKQTAGEMISKVKST</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VKM1</accession>
    <name>A0A6E8VKM1_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_040228807.1">
        <property type="nucleotide sequence ID" value="XM_040372873.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VKM1"/>
    <dbReference type="EnsemblMetazoa" id="ACON004943-RB">
        <property type="protein sequence ID" value="ACON004943-PB"/>
        <property type="gene ID" value="ACON004943"/>
    </dbReference>
    <dbReference type="GeneID" id="120953151"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001776"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004943"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033612"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006149">
        <property type="entry name" value="EB_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01683">
        <property type="entry name" value="EB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="230"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="EB" evidence="3">
        <location>
            <begin position="161"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="305"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004943-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="402" mass="45110" checksum="3C1F509B72E6C3A2" modified="2020-08-12" version="1">MIVSFFPDSVWGCTVKQHQKQKHLLYHKIIYTLISDKYKNRILRKSSFALLYLSYVFIKMLPVTLAIETHTGTKSITSLAAKTRPLPEMVAPPLDDEDDDYFDDYVDEYDIVNNKSAKGMYVGAPCEFTCHAKLHHVYCDPTTNTCSCEKDYVVLIGILKGCAKPKKLGEQCFYHQTCIYNDEHAMCVQIVHNAICQCAPGFHSVSYSKPTKRVFCTQDMATITADLPTLFGVTSGIAVLAGLICMVLHLFSKTKYPRPRHFGDANLAPPILFSSDTGSRRPSLASVHSTSSSVRSYSMRRFEQEIQQKELRQEMKRHLARLQEQQQLQKIKPNIIIGDVISCPMNKPILTTPSPMTPNSVDDILPSVDETQEFSPNLSQRSVSDSTGGCFDGPCSSTELLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VKM4</accession>
    <name>A0A6E8VKM4_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Sodium channel protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="22">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 18">Cell membrane</location>
            <topology evidence="1 18">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the sodium channel (TC 1.A.1.10) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON004707-RC">
        <property type="protein sequence ID" value="ACON004707-PC"/>
        <property type="gene ID" value="ACON004707"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON004707-RF">
        <property type="protein sequence ID" value="ACON004707-PF"/>
        <property type="gene ID" value="ACON004707"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010356"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004707"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034190"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001518">
        <property type="term" value="C:voltage-gated sodium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005248">
        <property type="term" value="F:voltage-gated sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0086010">
        <property type="term" value="P:membrane depolarization during action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019228">
        <property type="term" value="P:neuronal action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13433">
        <property type="entry name" value="Na_channel_gate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000061">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000046">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000047">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000019">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000022">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000023">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000026">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058542">
        <property type="entry name" value="IQ_SCN5A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001696">
        <property type="entry name" value="Na_channel_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044564">
        <property type="entry name" value="Na_chnl_inactivation_gate"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010526">
        <property type="entry name" value="Na_trans_assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024583">
        <property type="entry name" value="Na_trans_cytopl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043203">
        <property type="entry name" value="VGCC_Ca_Na"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10037:SF288">
        <property type="entry name" value="SODIUM CHANNEL PROTEIN PARA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10037">
        <property type="entry name" value="VOLTAGE-GATED CATION CHANNEL CALCIUM AND SODIUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24609">
        <property type="entry name" value="IQ_SCN5A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06512">
        <property type="entry name" value="Na_trans_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11933">
        <property type="entry name" value="Na_trans_cytopl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00170">
        <property type="entry name" value="NACHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="17 18" id="KW-0407">Ion channel</keyword>
    <keyword evidence="12 18" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0691">RNA editing</keyword>
    <keyword evidence="11 18" id="KW-0915">Sodium</keyword>
    <keyword evidence="3 18" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="16 18" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="7 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 18" id="KW-0813">Transport</keyword>
    <keyword evidence="9 18" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="133"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="204"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="238"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="262"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="393"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="762"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="806"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="835"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="890"/>
            <end position="912"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="970"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1280"/>
            <end position="1302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1308"/>
            <end position="1334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1355"/>
            <end position="1386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1473"/>
            <end position="1499"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1562"/>
            <end position="1580"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1592"/>
            <end position="1611"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1623"/>
            <end position="1642"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1686"/>
            <end position="1709"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1779"/>
            <end position="1799"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="21">
        <location>
            <begin position="1816"/>
            <end position="1851"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="34"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1111"/>
            <end position="1181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1966"/>
            <end position="2010"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="19">
        <location>
            <begin position="419"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="34"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="20">
        <location>
            <begin position="52"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="1138"/>
            <end position="1164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="20">
        <location>
            <begin position="1165"/>
            <end position="1181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="20">
        <location>
            <begin position="1978"/>
            <end position="2006"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU361132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004707-PF"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="2074" mass="232941" checksum="871F0E384D650939" modified="2020-08-12" version="1">MTEDSDSISEEERSLFRPFTRESLQAIEARIADEEAKQRELERKRAEGEIRYDDEDEDEGPQPDPTLEQGVPVPVRMQGSFPPELASTPLEDIDSFYSNQRTFVVISKGKDIFRFSATNALYVLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILQPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKEFPLDGSWGNLTDESWELFNSNDTNWFYSESGDIPLCGNSSGAGQCDEGYICLQGYGKNPNYGYTSFDTFGWAFLSAFRLMTQDYWENLYQLVLRSAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEALREAEEAAAAKAAKLEAQQAAAAAAANPEIAKSPSDFSCHSYELFVGQEKGNDDNNKEKMSIRSEGLESVSEITRTTAPTATAAGTAKARKVSAFTIRNGRGRFVGVPGSDRKPLVLSTYLDAQEHLPYADDSNAVTPMSEENGAIIVPVYYANLGSRHSSYTSHQSRISYTSHGDLLGGMTKESRLRNRSARNTNHSIVPPPNANNLSYAETNHKGQRDFDLTQDCTDDAGKIKHNDNPFIEPAQTQTVVDMKDVMVLNDIIEQAAGRHSRASDHGVSVYYFPTEDDDEDGPTFKDKALEFLMKMIDIFCVWDCCWVWLKFQEWVAFIVFDPFVELFITLCIVVNTLFMALDHHDMDPDMEKALKSGNYFFTATFAIEATMKLIAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYVDNVDRFPDHDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMLVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNETNKIAEAFNRISRFSNWIKMNLANALKFVKNKLTSQIASVQPTGKGVCPCISSEHGENELELTPDDILADGLLKKGIKEHNQLEVAIGDGMEFTIHGDLKNKAKKNKQIMNNSKDDDTASIKSYGSHKNRPFKDESHKGSAETMEGEEKRDASKEDLGIDEELDDEGEGDEGPLDGELIIHAEEDEVIEDSPADCCPDNCYKKFPVLAGDDDAPFWQGWGNLRLKTFQLIENKYFETAVITMILLSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFIIVMVSLINFVASLCGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCVDKNKTTLPHEIIPDVNACKAENYSWENSPMNFDHVGKAYLCLFQVATFKGWIQIMNDAIDSRDVGKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTNKKFDMIIMLFIGFNMLTMTLDHYKQSETFSAVLDYLNMIFICIFSSECLMKIFALRYHYFIEPWNLFDFVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKDKSGLDDVYNFKTFGQSMILLFQMSTSAGWDGVLDGIINEEDCLPPDNDKGYPGNCGSSTIGITYLLAYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPDGTQYVRYDQLSDFLDVLEPPLQIHKPNRYKIISMDIPICRGDMMFCVDILDALTKDFFARKGNPIEETAELGEVQQRPDEVGYEPVSSTLWRQREEYCARLIQHAWKRYKQRHGGGTDASGDDLEIDACDNGCGGGNGNENDDSGDGATGSGDNGSQHGGGSISGGGGTPGGGKSKGIIGSTQANIGIVDSNISPKESPDSIGDPQGRQTAVLVESDGFVTKNGHRVVIHSRSPSITSRTADV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VKM9</accession>
    <name>A0A6E8VKM9_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Sodium channel protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="22">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 18">Cell membrane</location>
            <topology evidence="1 18">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the sodium channel (TC 1.A.1.10) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON004707-RK">
        <property type="protein sequence ID" value="ACON004707-PK"/>
        <property type="gene ID" value="ACON004707"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010356"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004707"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034190"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001518">
        <property type="term" value="C:voltage-gated sodium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005248">
        <property type="term" value="F:voltage-gated sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0086010">
        <property type="term" value="P:membrane depolarization during action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019228">
        <property type="term" value="P:neuronal action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13433">
        <property type="entry name" value="Na_channel_gate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000061">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000046">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000047">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000019">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000022">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000023">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000026">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058542">
        <property type="entry name" value="IQ_SCN5A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001696">
        <property type="entry name" value="Na_channel_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044564">
        <property type="entry name" value="Na_chnl_inactivation_gate"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010526">
        <property type="entry name" value="Na_trans_assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024583">
        <property type="entry name" value="Na_trans_cytopl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043203">
        <property type="entry name" value="VGCC_Ca_Na"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10037:SF288">
        <property type="entry name" value="SODIUM CHANNEL PROTEIN PARA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10037">
        <property type="entry name" value="VOLTAGE-GATED CATION CHANNEL CALCIUM AND SODIUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24609">
        <property type="entry name" value="IQ_SCN5A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06512">
        <property type="entry name" value="Na_trans_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11933">
        <property type="entry name" value="Na_trans_cytopl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00170">
        <property type="entry name" value="NACHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="17 18" id="KW-0407">Ion channel</keyword>
    <keyword evidence="12 18" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0691">RNA editing</keyword>
    <keyword evidence="11 18" id="KW-0915">Sodium</keyword>
    <keyword evidence="3 18" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="16 18" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="7 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 18" id="KW-0813">Transport</keyword>
    <keyword evidence="9 18" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="133"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="204"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="238"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="262"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="393"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="790"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="834"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="863"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="918"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="998"/>
            <end position="1019"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1308"/>
            <end position="1330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1336"/>
            <end position="1362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1383"/>
            <end position="1414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1501"/>
            <end position="1527"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1590"/>
            <end position="1608"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1620"/>
            <end position="1639"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1651"/>
            <end position="1670"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1714"/>
            <end position="1737"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1807"/>
            <end position="1827"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="21">
        <location>
            <begin position="1844"/>
            <end position="1879"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="34"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1139"/>
            <end position="1209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1994"/>
            <end position="2038"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="19">
        <location>
            <begin position="419"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="34"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="20">
        <location>
            <begin position="52"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="1166"/>
            <end position="1192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="20">
        <location>
            <begin position="1193"/>
            <end position="1209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="20">
        <location>
            <begin position="2006"/>
            <end position="2034"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU361132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004707-PK"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="2102" mass="235845" checksum="30CFA5F9C4F7ADFC" modified="2020-08-12" version="1">MTEDSDSISEEERSLFRPFTRESLQAIEARIADEEAKQRELERKRAEGEIRYDDEDEDEGPQPDPTLEQGVPVPVRMQGSFPPELASTPLEDIDSFYSNQRTFVVISKGKDIFRFSATNALYVLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILQPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKEFPLDGSWGNLTDESWELFNSNDTNWFYSESGDIPLCGNSSGAGQCDEGYICLQGYGKNPNYGYTSFDTFGWAFLSAFRLMTQDYWENLYQLVLRSAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEALREAEEAAAAKAAKLEAQQAAAAAAANPEIAKSPSDFSCHSYELFVGQEKGNDDNNKEKMSIRSEGLESVSEITRTTAPTATAAGTAKARKVSAGVATIQKASLSLPGSPFNLRRGSRGSHQFTIRNGRGRFVGVPGSDRKPLVLSTYLDAQEHLPYADDSNAVTPMSEENGAIIVPVYYANLGSRHSSYTSHQSRISYTSHGDLLGGMTKESRLRNRSARNTNHSIVPPPNANNLSYAETNHKGQRDFDLTQDCTDDAGKIKHNDNPFIEPAQTQTVVDMKDVMVLNDIIEQAAGRHSRASDHGVSVYYFPTEDDDEDGPTFKDKALEFLMKMIDIFCVWDCCWVWLKFQEWVAFIVFDPFVELFITLCIVVNTLFMALDHHDMDPDMEKALKSGNYFFTATFAIEATMKLIAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYVDNVDRFPDHDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMLVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNETNKIAEAFNRISRFSNWIKMNLANALKFVKNKLTSQIASVQPTGKGVCPCISSEHGENELELTPDDILADGLLKKGIKEHNQLEVAIGDGMEFTIHGDLKNKAKKNKQIMNNSKDDDTASIKSYGSHKNRPFKDESHKGSAETMEGEEKRDASKEDLGIDEELDDEGEGDEGPLDGELIIHAEEDEVIEDSPADCCPDNCYKKFPVLAGDDDAPFWQGWGNLRLKTFQLIENKYFETAVITMILLSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFIIVMVSLINFVASLCGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCVDKNKTTLPHEIIPDVNACKAENYSWENSPMNFDHVGKAYLCLFQVATFKGWIQIMNDAIDSRDVGKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTNKKFDMIIMLFIGFNMLTMTLDHYKQSETFSAVLDYLNMIFICIFSSECLMKIFALRYHYFIEPWNLFDFVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKDKSGLDDVYNFKTFGQSMILLFQMSTSAGWDGVLDGIINEEDCLPPDNDKGYPGNCGSSTIGITYLLAYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPDGTQYVRYDQLSDFLDVLEPPLQIHKPNRYKIISMDIPICRGDMMFCVDILDALTKDFFARKGNPIEETAELGEVQQRPDEVGYEPVSSTLWRQREEYCARLIQHAWKRYKQRHGGGTDASGDDLEIDACDNGCGGGNGNENDDSGDGATGSGDNGSQHGGGSISGGGGTPGGGKSKGIIGSTQANIGIVDSNISPKESPDSIGDPQGRQTAVLVESDGFVTKNGHRVVIHSRSPSITSRTADV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VLZ5</accession>
    <name>A0A6E8VLZ5_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Aldehyde dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4 7">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VLZ5"/>
    <dbReference type="EnsemblMetazoa" id="ACON005124-RD">
        <property type="protein sequence ID" value="ACON005124-PD"/>
        <property type="gene ID" value="ACON005124"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006213"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005124"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031069"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004029">
        <property type="term" value="F:aldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006081">
        <property type="term" value="P:aldehyde metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07132">
        <property type="entry name" value="ALDH_F3AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000003">
        <property type="entry name" value="Aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000004">
        <property type="entry name" value="Aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012394">
        <property type="entry name" value="Aldehyde_DH_NAD(P)"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43570">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43570:SF16">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE TYPE III, ISOFORM Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036492">
        <property type="entry name" value="ALDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="2 4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="469"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="9">
        <location>
            <begin position="7"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="active site" evidence="5 6">
        <location>
            <position position="209"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR036492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR036492-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005124-PD"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="495" mass="55283" checksum="90A5A006C6350468" modified="2020-08-12" version="1">MSFSDIVQQLRTTFNSGKTRDVDFRLNQLKALLRCYEENTAEMANVLAADLRKHKQEAHLLEIEFILNDLRNTLYNLREWVKPEKPEKSLVNVMDGVYIYKDPYGVVLVIGAWNYPLQLTLVPVAAAIAAGNCVVIKPSEVAPASAKFIAETLPKYLSQDCYRVVTGGPKETSELLKEKFDYVFYTGSGRVGKIIHQACSENLTPCTLELGGKSPCYLDGTANIAIAARRILWGKFINAGQTCIAPDYILCSKQVQKQFLEEARKVLNEWYGANPKDSPDLCRIINQNHFQRLTSLLKGANVAIGGETDLQDRYISPTILIDVKSTDPVMQDEIFGPILPILNVDNAYDAIKFINSRPPPLVMYIFTLDTKIQELFVTGTRSGSMCLNDTIMQYAVEALPFGGVGPSGMGSYHGKYSFDTFVHKKSCLTKDFNPIGEKLAASRYPPYSESKLSFLSTLLKKRQGINLHFLPYLLMFGIGVASTLVVSTILKRRLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VLZ7</accession>
    <name>A0A6E8VLZ7_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">LIM zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_040222295.1">
        <property type="nucleotide sequence ID" value="XM_040366361.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VLZ7"/>
    <dbReference type="EnsemblMetazoa" id="ACON005400-RA">
        <property type="protein sequence ID" value="ACON005400-PA"/>
        <property type="gene ID" value="ACON005400"/>
    </dbReference>
    <dbReference type="GeneID" id="120949215"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012550"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005400"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037772"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09430">
        <property type="entry name" value="LIM5_LIMPETin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09432">
        <property type="entry name" value="LIM6_LIMPETin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000013">
        <property type="entry name" value="Four and a half LIM domains 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000070">
        <property type="entry name" value="Four and a half LIM domains 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000066">
        <property type="entry name" value="Four and a half LIM domains protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24205">
        <property type="entry name" value="FOUR AND A HALF LIM DOMAINS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24205:SF4">
        <property type="entry name" value="PROTEIN ESPINAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0440">LIM domain</keyword>
    <keyword evidence="1 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="7">
        <location>
            <begin position="63"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="7">
        <location>
            <begin position="123"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="7">
        <location>
            <begin position="185"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005400-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="244" mass="27838" checksum="B77F12570EE9AD5D" modified="2020-08-12" version="1">MAMDILSSDFDSRLRLKTKTIGGERIKRAKDNNEDIAILSMFLPGATAAKEQEKFRCHLGMDCRVGDCRDTTPGTKKMEYKTRQWHEKCFCCCVCKTAIGTKSFIPREQEIYCAGCYEEKYATRCIKCKKIITSGGVTYKNEPWHRECFTCTHCQVSLAGQRFTSRDEKPYCAECFGELFAKRCTSCTKPITGIGGTRFISFEDRHWHNDCFICAMCKTSLVGRGFITDEQDVICPECAKQKLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VN29</accession>
    <name>A0A6E8VN29_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Autophagy-related protein 13</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Preautophagosomal structure</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the ATG13 family. Metazoan subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VN29"/>
    <dbReference type="EnsemblMetazoa" id="ACON005715-RA">
        <property type="protein sequence ID" value="ACON005715-PA"/>
        <property type="gene ID" value="ACON005715"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_008546"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005715"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034600"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990316">
        <property type="term" value="C:Atg1/ULK1 kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034497">
        <property type="term" value="P:protein localization to phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.900.10">
        <property type="entry name" value="HORMA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040182">
        <property type="entry name" value="ATG13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018731">
        <property type="entry name" value="Atg13_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036570">
        <property type="entry name" value="HORMA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13430:SF4">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10033">
        <property type="entry name" value="ATG13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0072">Autophagy</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Autophagy-related protein 13 N-terminal" evidence="6">
        <location>
            <begin position="2"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="203"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="267"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="211"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="226"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF10033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005715-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="417" mass="46195" checksum="58E4A779698CA406" modified="2020-08-12" version="1">MILECWTLSIRTDQTNAPQRANQVIYNRMSLLLKSLLSVTRVTPAYRVSRMKRADSYDIYYRIYTGEPQTNQLGDGYNQVRVGQLCTQIGTLNMAVAYRTKITFSPTQTGRDNTIMLKSDHFLKDISPKHVRYNYNKKIEKKVIDLDKPMRCGAFVDASRVKKPTEDDYTLPETPPFSWLLPKPRDESTFRGGEGCEDRVSLHSPTNVDENCSSNANNLNNNNASKGSYESNSGPMHFRLSQSPKSGDAIATGAIPVPGAATVAMPAAATTTGSSTPPPSSLGGAGSELRRSSRWSIRRDPSENDRMLKELQFAFAETTAQGELAKFLRECSNAPTLHEFDVMRSSGADTLTEEDHIIDGSRILTEADTCPDSGEDEEEGDVLADLTRQLEQFETSLPEYEDLVTSLCQSIDSNSNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VP30</accession>
    <name>A0A6E8VP30_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Laccase 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the multicopper oxidase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040222845.2">
        <property type="nucleotide sequence ID" value="XM_040366911.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VP30"/>
    <dbReference type="EnsemblMetazoa" id="ACON006176-RA">
        <property type="protein sequence ID" value="ACON006176-PA"/>
        <property type="gene ID" value="ACON006176"/>
    </dbReference>
    <dbReference type="GeneID" id="120949541"/>
    <dbReference type="CTD" id="35494"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007077"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON006176"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031796"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13858">
        <property type="entry name" value="CuRO_1_tcLCC2_insect_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13884">
        <property type="entry name" value="CuRO_2_tcLCC_insect_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13905">
        <property type="entry name" value="CuRO_3_tcLLC2_insect_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000045">
        <property type="entry name" value="Laccase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000073">
        <property type="entry name" value="Laccase 2, isoform E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000031">
        <property type="entry name" value="Laccase-2 isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001117">
        <property type="entry name" value="Cu-oxidase_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033138">
        <property type="entry name" value="Cu_oxidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002355">
        <property type="entry name" value="Cu_oxidase_Cu_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF232">
        <property type="entry name" value="STRAW, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00394">
        <property type="entry name" value="Cu-oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00079">
        <property type="entry name" value="MULTICOPPER_OXIDASE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00080">
        <property type="entry name" value="MULTICOPPER_OXIDASE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026037398" description="Laccase 2" evidence="5">
        <location>
            <begin position="25"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="8">
        <location>
            <begin position="198"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="6">
        <location>
            <begin position="324"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="7">
        <location>
            <begin position="581"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="75"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="84"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON006176-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="754" mass="83869" checksum="2BFBBF1396B9B717" modified="2020-08-12" version="1">MAIDWRNRALSLGILLALAVAADAVRVQQHTSRRFKDESFGHDQTPAGSWWNSHLTEPPSKNFYQATHGLLQTHPSVPSLKPVAGAPAAPGPSALPLSSRKAPTVSSAAALNSGFPSIANPNPRSPFRHLDFSTSATAELRRNPSLSAPDECARACREGEPPRICYYHFTVEYYTVLGAACQVCTPNATNTVWSHCQCVLADGVERGILTVNRMIPGPSIQVCENDRVVIDVENHMEGMELTIHWHGIWQRGTQYYDGVPFVTQCPIQQGNTFRYQWTGNAGTHFWHAHTGLQKLDGLYGSIVVRQPPSRDPNSHLYDFDLTTHIMLVSDWLHEDAAERYPGRLAVNTGQDPESLLINGKGQFRDPNTGFMTNTPLEIFTITPGRRYRFRMINAFASVCPAQVTIEGHALTVIATDGEPVHPVQVNTIISFSGERYDFVITADQPVGAYWIQLRGLGECGIKRAQQLAILRYARGPYQPASPPPTYDVGLPQGVVMNPLDAQCNVQRDDAICVSQLKNAKEIDRALLQEKPDVKIFLPFRFYLYRPEELFQPNTYNRFLVAPTGDHVISLIDEISYLSAPAPLLSQYDDINPEQFCNGDNRPADCGANCMCTHKVDIPLNAIVEVVLVDEVQQPNLSHPFHLHGYAYNVIGIGRSPDSNVKKINLKHALDLDRRGLLHRQYNLPPLKDTIAVPNNGYVVLRFRADNPGFWLFHCHFLFHIVIGMNLILQVGTHADLPPVPPNFPTCGDHLPPIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VP33</accession>
    <name>A0A6E8VP33_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the troponin C family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040228051.1">
        <property type="nucleotide sequence ID" value="XM_040372117.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VP33"/>
    <dbReference type="EnsemblMetazoa" id="ACON006178-RC">
        <property type="protein sequence ID" value="ACON006178-PC"/>
        <property type="gene ID" value="ACON006178"/>
    </dbReference>
    <dbReference type="GeneID" id="120952667"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006741"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON006178"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033130"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000103">
        <property type="entry name" value="Troponin C Ib"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000439">
        <property type="entry name" value="Troponin C type IIIb1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF0">
        <property type="entry name" value="CALMODULIN LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="7">
        <location>
            <begin position="23"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="7">
        <location>
            <begin position="59"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="7">
        <location>
            <begin position="100"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="7">
        <location>
            <begin position="136"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON006178-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="167" mass="18669" checksum="5531322B65E04C1C" modified="2020-08-12" version="1">MSDTASEAGESTSSGAGKELSKDQLKVLRDAFNAFDKEKTGSIPTDVVGTILELLGHKLSEEELDEVIEEYDEDESGQLEFEEFVALASNYVEPEEDYEALRKELREVFMMYDKDAKGYLPVEEFKAILRELDGAVPEEELDDIVDEIDADGSGTVDFEEFMEVMTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VP39</accession>
    <name>A0A6E8VP39_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Calcium-transporting ATPase</fullName>
            <ecNumber evidence="21">7.2.2.10</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="23">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="21">Catalyzes the hydrolysis of ATP coupled with the transport of calcium.</text>
    </comment>
    <comment type="function">
        <text evidence="20">This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>Ca(2+)(in) + ATP + H2O = Ca(2+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18105"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.10"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="21">Membrane</location>
            <topology evidence="21">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Sarcoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18 21">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.10" evidence="21"/>
    <dbReference type="RefSeq" id="XP_040226657.1">
        <property type="nucleotide sequence ID" value="XM_040370723.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VP39"/>
    <dbReference type="EnsemblMetazoa" id="ACON006186-RA">
        <property type="protein sequence ID" value="ACON006186-PA"/>
        <property type="gene ID" value="ACON006186"/>
    </dbReference>
    <dbReference type="GeneID" id="120951795"/>
    <dbReference type="CTD" id="49297"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000733"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON006186"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041260"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033017">
        <property type="term" value="C:sarcoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005388">
        <property type="term" value="F:P-type calcium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd02083">
        <property type="entry name" value="P-type_ATPase_SERCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000143">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000003">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000005">
        <property type="entry name" value="Calcium-transporting ATPase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000065">
        <property type="entry name" value="Sarcoplasmic/endoplasmic reticulum calcium ATPase 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005782">
        <property type="entry name" value="P-type_ATPase_IIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01116">
        <property type="entry name" value="ATPase-IIA1_Ca"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42861">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08282">
        <property type="entry name" value="Hydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 21" id="KW-0106">Calcium</keyword>
    <keyword evidence="5 21" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="16 21" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="17 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0703">Sarcoplasmic reticulum</keyword>
    <keyword evidence="14" id="KW-1278">Translocase</keyword>
    <keyword evidence="6 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 21" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="60"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="90"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="259"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="297"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="759"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="792"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="832"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="927"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="22">
        <location>
            <begin position="3"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00038148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00054278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU361146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON006186-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1001" mass="109965" checksum="FD631668C3A59224" modified="2020-08-12" version="1">MEDGHSKTVDEVLSHFRVDPERGLSLDQVKEYQKKYGPNELPAEEGKTLWQLVLEQFDDLLVKILLLAAIISFVLALFEEHEGVEAFVEPFVILLILIANAVVGVWQERNAESAIEALKEYEPEMGKVIRGDKSGVQKIRAKEIVPGDVVEVSVGDKIPADIRLIKIYSTTIRIDQSILTGESVSVIKHTDAVPDPRAVNQDKKNILFSGTNVAAGKARGVVIGTGLNTAIGKIRTEMSETEEIKTPLQQKLDEFGEQLSKVISLICVAVWAINIGHFNDPAHGGSWIKGAVYYFKIAVALAVAAIPEGLPAVITTCLALGTRRMAKKNAIVRSLPSVETLGCTSVICSDKTGTLTTNQMSVSRMFIFEKIEGNDSSFTEFEISGSTYEPIGEVTLNGQRIKAADYETLHELGTICIMCNDSAIDFNETKKVFEKVGEATETALIVLAEKLNPFNVAKQGLDRRSSAICVRQEIETKWKKEFTLEFSRDRKSMSSYCTPLKASKLGNGPKLFCKGAPEGVLERCTHARVGSTKVPLTQTLKQRILDLTRTYGTGRDTLRCLALATADSPMKPDDMDLNDSTKFYTYEVNLTFVGVVGMLDPPRKEVQDSIVRCRAAGIRVIVITGDNKATAEAICRRIGVFGEDEDTTGKSYSGREFDDLSVSEQREACSRARLFSRVEPAHKSKIVEFLQSMNEISAMTGDGVNDAPALKKAEIGIAMGSGTAVAKSAAEMVLADDNFSSIVAAVEEGRAIYNNMKQFIRYLISSNIGEVVSIFLTAALGLPEALIPVQLLWVNLVTDGLPATALGFNPPDLDIMTKPPRKADEGLISGWLFFRYMAIGGYVGCATVGGAAWWFMFSETGPQLSYWQLTHHLSCLGGGEEFKGIDCKIFNDPHPMTMALSVLVTIEMLNAMNSLSENQSLVQMPPWCNIWLIASMCLSFALHFVILYVDVLSTVFQVTPLDGNEWMTVMKFSLPVVLLDEILKFVARRISDANEVIKTWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VP43</accession>
    <name>A0A6E8VP43_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VP43"/>
    <dbReference type="EnsemblMetazoa" id="ACON006103-RA">
        <property type="protein sequence ID" value="ACON006103-PA"/>
        <property type="gene ID" value="ACON006103"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005691"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON006103"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034640"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006616">
        <property type="entry name" value="DM9_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31649">
        <property type="entry name" value="AGAP009604-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31649:SF10">
        <property type="entry name" value="IP19903P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11901">
        <property type="entry name" value="DM9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00696">
        <property type="entry name" value="DM9"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON006103-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="145" mass="15881" checksum="64DB494F47DF9BA1" modified="2020-08-12" version="1">MITGCWQHCNVNGPFPPNMVRAGVDSDGEVIYVGRAFHEGDMVPAKVIPTKNVAFVCHGGEEVLKEDFEVLRYGAFVWEYSSNGSVPETAMRIGQTMDGEPLYMGRAIYSGSQTPGKVHPSHGCCYLPFDGAEVSVTDYEVLCIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VQD3</accession>
    <name>A0A6E8VQD3_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Gustatory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Gustatory receptor which mediates acceptance or avoidance behavior, depending on its substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the insect chemoreceptor superfamily. Gustatory receptor (GR) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VQD3"/>
    <dbReference type="EnsemblMetazoa" id="ACON006143-RC">
        <property type="protein sequence ID" value="ACON006143-PC"/>
        <property type="gene ID" value="ACON006143"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACON006143"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032593"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007635">
        <property type="term" value="P:chemosensory behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008049">
        <property type="term" value="P:male courtship behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050909">
        <property type="term" value="P:sensory perception of taste"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013604">
        <property type="entry name" value="7TM_chemorcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21143:SF133">
        <property type="entry name" value="GUSTATORY AND PHEROMONE RECEPTOR 32A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21143">
        <property type="entry name" value="INVERTEBRATE GUSTATORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08395">
        <property type="entry name" value="7tm_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="73"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="131"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="166"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="232"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="265"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="351"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON006143-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="376" mass="43752" checksum="5126B3EF55D0FC1B" modified="2020-08-12" version="1">MDELQFLNVFNYCFVSPTYLSFDIENNRYDFRLGNLRVCWNVTLLIVLVTVNCIGATVMVLEFMQDQLDSVMGACNIIICIMRLSVVIPLILWIWLYRHKVLEVCTIALNLLQDKSKLQFQNPTKRNSRCLLYWMQVGTIVILLIAAVVGDVYIWWKFIAKQKLYYILNLSAYIILDFVSLMHLMYTQCLALVIADHFHELACRVKNEPDEPDILQTVNTIWDVLEHYKQRIIATFGVMNVLHALDALVKCAVETYVIFSTWEMGFGLLGAFLDIITLTVYAVTFFIFAFAHDRVEVKEAELKDALKSMQYTNLKRQSRDQKDFYDLVNLKLMMESPKITACGLFEINLQIFYNVFAAIITYIVILFQFRGFEKSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VQD6</accession>
    <name>A0A6E8VQD6_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">MOG interacting and ectopic P-granules protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Nuclear zinc finger protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Has a broad role in development, specifically in the genetic pathway SynMuvB that negatively regulates specification of the vulval cell fate. Required for fem-3 3'-UTR-mediated repression in the regulation of the sperm/oocyte switch. Acts by regulating the translation of fem-3 mRNA, by binding to its 3'-UTR.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Interacts with hda-1, let-418, lin-1, mog-1, mog-4, mog-5, mog-6, pie-1 and unc-98.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_040223261.2">
        <property type="nucleotide sequence ID" value="XM_040367327.2"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON006601-RA">
        <property type="protein sequence ID" value="ACON006601-PA"/>
        <property type="gene ID" value="ACON006601"/>
    </dbReference>
    <dbReference type="GeneID" id="120949802"/>
    <dbReference type="CTD" id="38327"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010522"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON006601"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037700"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="PANTHER" id="PTHR24403:SF106">
        <property type="entry name" value="PR_SET DOMAIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24403">
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    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
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        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
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    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
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    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
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            <begin position="1285"/>
            <end position="1306"/>
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            <begin position="1323"/>
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</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VQE1</accession>
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        <submittedName>
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        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
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        <lineage>
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            <taxon>Hexapoda</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
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            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">Ngousso</strain>
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        <property type="gene ID" value="ACON006721"/>
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    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038093"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="FN3_sf"/>
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        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21104">
        <property type="entry name" value="FIBRONECTIN TYPE III DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
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        <property type="entry name" value="FIBRONECTIN TYPE-III DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF16066">
        <property type="entry name" value="DUF4808"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
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            <begin position="1"/>
            <end position="17"/>
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            <begin position="18"/>
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            <begin position="119"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VQW8</accession>
    <name>A0A6E8VQW8_ANOCL</name>
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            <fullName evidence="15">2-oxoglutarate dehydrogenase, mitochondrial</fullName>
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        </recommendedName>
        <alternativeName>
            <fullName evidence="16">2-oxoglutarate dehydrogenase complex component E1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Alpha-ketoglutarate dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the alpha-ketoglutarate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.4.2" evidence="5"/>
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        <property type="nucleotide sequence ID" value="XM_040367298.2"/>
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    <dbReference type="AlphaFoldDB" id="A0A6E8VQW8"/>
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        <property type="protein sequence ID" value="ACON006366-PD"/>
        <property type="gene ID" value="ACON006366"/>
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        <property type="protein sequence ID" value="ACON006366-PE"/>
        <property type="gene ID" value="ACON006366"/>
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    <dbReference type="GeneID" id="120949786"/>
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    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006013"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:mitochondrion"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:oxoglutarate dehydrogenase complex"/>
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        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="term" value="P:glycolytic process"/>
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        <property type="term" value="P:tricarboxylic acid cycle"/>
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        <property type="entry name" value="TPP_E1_OGDC_like"/>
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        <property type="entry name" value="Multifunctional 2-oxoglutarate metabolism enzyme, C-terminal domain"/>
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        <property type="entry name" value="2-oxogl_dehyd_N"/>
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        <property type="entry name" value="2oxoglutarate_DH_E1"/>
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        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Transketolase-like pyrimidine-binding" evidence="17">
        <location>
            <begin position="651"/>
            <end position="864"/>
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    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1014" mass="114013" checksum="6E55BCCFF19A655C" modified="2020-08-12" version="1">MHRARTALHMVNPMGQQNFGSFLLKKQASKLTTELVAASSVKLYNSAAAEPFLNGSSSNYIDDMYNAWLRDPASVHASWDAYFRNNSYAAPPSLAPVPKNHVPAAQYLGSSLPAVAGAGAAVGGRIDDKLIDDHLAVQAIIRSYQIRGHNVARLDPLGINSADLDDKTPPELLYSSYRFEEADMERVFKLPSTTFIGGKEKFLPLREILGRLEKAYCNKIGVEFMFINSLEQCNWIRERFETPNIMTYTNEEKRLILARLTRATGFEAFLAKKFSSEKRFGLEGCEIMIPAMKEVIDVSTRLGVESIIMGMPHRGRLNVLANVCRKPLHQIFTQFAGLEAADDGSGDVKYHLGTYIERLNRVTNKNIRLAVVANPSHLEAVDPVVQGKTRAEQFYRGDGEGKKVMSILLHGDAAFCGQGVVFETMHLSDLPDYTTHGTIHIVVNNQIGFTTDPRHSRSSPYCTDVARVVNAPIFHVNGDDPEAVMHVCKVAAEWRATFHKDVIIDIVSYRRNGHNEIDEPMFTQPLMYKKIRGTKPALDIYANQLITEGVVTAEEVKSVKDKYEKICEEAFEQAKIETHIKYKDWIDSPWSGFFEGKDPLKVAPTGVIEETLVHIGNRFSSPPPNAAEFVIHKGLLRVLAARKEMLENKTIDWALAEAMAFGSLLKEGIHVRLSGQDVERGTFSHRHHVLHHQTVDKATYRPLCHLYPDQAPYTVCNSSLSEFGVLGFELGYSMTNPNALVCWEAQFGDFNNTAQCIIDQFVSSGQAKWVRQSGLVMLLPHGMEGMGPEHSSARVERFLQMCSDDPDYFPPESEEFAIRQLHDINWIVANCSTPGNYFHLLRRQIALPFRKPLIVLTPKSLLRHPECRSNFSEMTDGTEFKRLIPDALTAENPNQVKRVIFCTGRVYYDLLKARRDRKLDHDIAISRIEQISPFPYDLVKAECAKYPNAELVWAQEEHKNQGCWTYVQPRFDTAINSTRDFSYVGRPCGASTATGSKAQHLKELKNLLDDAMAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VQY4</accession>
    <name>A0A6E8VQY4_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="3">USP domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_040224279.2">
        <property type="nucleotide sequence ID" value="XM_040368345.2"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON006652-RA">
        <property type="protein sequence ID" value="ACON006652-PA"/>
        <property type="gene ID" value="ACON006652"/>
    </dbReference>
    <dbReference type="GeneID" id="120950366"/>
    <dbReference type="CTD" id="9100"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011079"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON006652"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034244">
        <property type="term" value="P:negative regulation of transcription elongation by RNA polymerase II"/>
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    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Papain-like_cys_pep_sf"/>
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        <property type="entry name" value="LD28815P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13328">
        <property type="entry name" value="NEGATIVE ELONGATION FACTOR A NELF-A"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="USP_3"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="USP" evidence="2">
        <location>
            <begin position="1368"/>
            <end position="1746"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="607"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="776"/>
            <end position="843"/>
        </location>
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        <location>
            <begin position="873"/>
            <end position="911"/>
        </location>
    </feature>
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        <location>
            <begin position="923"/>
            <end position="1021"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1100"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1215"/>
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        </location>
    </feature>
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        <location>
            <begin position="1429"/>
            <end position="1454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1748"/>
            <end position="1850"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="70"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="210"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="227"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="270"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="560"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="783"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="798"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="829"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="877"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="933"/>
            <end position="960"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="982"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1100"/>
            <end position="1126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1131"/>
            <end position="1147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1234"/>
            <end position="1260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1288"/>
            <end position="1323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1442"/>
            <end position="1451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1760"/>
            <end position="1789"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1806"/>
            <end position="1817"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1824"/>
            <end position="1836"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON006652-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1850" mass="193532" checksum="893936D05A56DC5C" modified="2020-08-12" version="1">MPPHTNNPHHLQQQQQQQQQQQQQLGSAANNNQQQQQTGGQQHQQPGPPQSQPHNVGGRGGGGGAGGSAGSVTAGADGVGRNTDRDNGNQQMQQQAVSVANQHQQQMMQQVYAQIPASAYMQPTPHGMYPVQVLPPTAGPVPNNVFVSNLTANVSVHGYPAISPYLATATPPGAYMPADVGAGPEMQLMPPAPVTMSGRGTMRRGGRNNRGGNNSRSRGGYVPHYVQQHQQQSYHHQQQQQHQQSYHQALQHQQQQQQQQGVMQPLSHHQAGQQAALQHQVQQQQQQQQQQQQQQQQAQQGQGGGGAGQQQTPPTAGSGGGQQGQQQQQQHPPPQHTPELMHIEAPPMMGGAGAQYGNLHGGFYVIPSMAQHASGAPLFMPAPHMPMYYNPAIHAYQNLIYPPYIPSEYQMYDDGKGDDGGGPHGGQGGGHDDPGVHPGAAMWPQPPPIALDYHPETVEYLPVHEDDGAAGGMLPPGPPPQAIPPPAMGHHVLDPNVPGFTMQMAAAPIATMQPPPEEYIMQQQPPPAPQQQQQQQQPHAGAAGSQTQGEDYNGNGGIPVSSNSNTTMHSPVVATGQHNVVVPGVPPPEDMMGAGGYVHGGAAPQQPMVPAPAEPQQQHGQTMDGAGAQPPMNNNFIVEPQYIQAHPLPPHHMPVQQQQQVPPEQHYQHIPPPQRAAVNVTEPPPSTNNQPFQMAQQTPVAAAGVVANNLLDANGNDTNNNNSANRKSDIITSPKLVDAVVMTHPSPLPSTVNVATATPSHHQQNEQTAAVVQGGMMDGNAGRKQSNSSDVPNQGFLPPQQQQHQQQPPMLGYAAAVASQAPPPRGRAEQQQQQPDSVKATQDRMEKLTLKEQEHRRTTTTVAAAAAAGTVVGGGASQRTSGAQPVAGQSNAGSGWVNNSGGAGPKKGTASVSVSAIPNKEFPGSVIGHQHKNTSPVPFSTLVGTTATAPPSTVMGSASGKQPHAVAAAAAGPKSFPDHGQRSSGVANSGQSTYASGANSSMSGGPTAVVTPQMESKKVEAIPQRTVATTAVAAAAVTGPAPTAPVGGAVVDTPKQHHQDVVNVPRPAASSISSSTNINTVKSATKAPVIINHPLNIPPPDHPLNIPPPIIVPPPQIPPGGAPPQPNKTWASLFQTSSSSSSSSSSSAAVASSASSSAALSVSASAVTATSATAVSSVPPSAALSTASASGPAAGQSGVASIGTTVATAPHHHATAPVTSTGINFAPPTANRGPAMAASTAPPTAAVGNTPSSIDSSSSSTGAKKPVAKVQPYDRNHQTPATPAVPMSYSSAAASTPASGTTGGSTPTSANAARGKGAQQAAASKGAANATAASASTAGDQKDEFSLKFGDFLSGYSIDNTNISMMPRGLINRSNYCYINTILQALIACPPFYHLMRTIRTLPAAKNSKHPKPFIDAMCALANEFSQLPIRSKPQQQQQRGGGDKGKKDDIPEIQVDTPFEPTVIYKMLNGIRSDIFQIEGRQEDAEEFLGCVLNRLNDEMIEFIKFNKTDQGNLNGEEPTNGEVHGEEDADDWMVICKGNKGTVTRTTDFGRSPISDIFRGKLRSRVQRDGVPPTYNIQPFFTLPLDIEKAASVKEALEQLVGRDELEGVTCSKTKQEVAAWQQVTLEELPVVLILHLKCFDYKMDGCTKILKTLDFPIELKIDSKLMSSKGKSYSAKQKQYKLFAVVYHDGKEASKGHYITDVYHTGYASWIRYDDSIVKPVPEYNVLRPRAPRVPYLLYYRRMDTQTHGPNSDRGGDGSTGGGAGSGGGGSGSGRGGSSHSTGGGGNDRHSAHHHSNDHHGRGSSGGGYGGSSGYGNTHGNSGGNHYGSGGHGQSNHNSGHYGGNSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VQY7</accession>
    <name>A0A6E8VQY7_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cytochrome c1, heme protein, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Complex III subunit 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Complex III subunit IV</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Cytochrome b-c1 complex subunit 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit</fullName>
        </alternativeName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="24">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="21">
            <name>heme c</name>
            <dbReference type="ChEBI" id="CHEBI:61717"/>
        </cofactor>
        <text evidence="21">Binds 1 heme c group covalently per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="19">Component of the ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII), a multisubunit enzyme composed of 11 subunits. The complex is composed of 3 respiratory subunits cytochrome b, cytochrome c1 and Rieske protein UQCRFS1, 2 core protein subunits UQCRC1/QCR1 and UQCRC2/QCR2, and 6 low-molecular weight protein subunits UQCRH/QCR6, UQCRB/QCR7, UQCRQ/QCR8, UQCR10/QCR9, UQCR11/QCR10 and subunit 9, the cleavage product of Rieske protein UQCRFS1. The complex exists as an obligatory dimer and forms supercomplexes (SCs) in the inner mitochondrial membrane with NADH-ubiquinone oxidoreductase (complex I, CI) and cytochrome c oxidase (complex IV, CIV), resulting in different assemblies (supercomplex SCI(1)III(2)IV(1) and megacomplex MCI(2)III(2)IV(2)). Interacts with FLVCR2; this interaction occurs in the absence of heme and is disrupted upon heme binding.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cytochrome c family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VQY7"/>
    <dbReference type="EnsemblMetazoa" id="ACON006936-RB">
        <property type="protein sequence ID" value="ACON006936-PB"/>
        <property type="gene ID" value="ACON006936"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_013781"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON006936"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037126"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006122">
        <property type="term" value="P:mitochondrial electron transport, ubiquinol to cytochrome c"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.760.10:FF:000002">
        <property type="entry name" value="Cytochrome c1, heme protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.100:FF:000003">
        <property type="entry name" value="Cytochrome c1, heme protein, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.760.10">
        <property type="entry name" value="Cytochrome c-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.100">
        <property type="entry name" value="Cytochrome c1, transmembrane anchor, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009056">
        <property type="entry name" value="Cyt_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036909">
        <property type="entry name" value="Cyt_c-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002326">
        <property type="entry name" value="Cyt_c1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021157">
        <property type="entry name" value="Cyt_c1_TM_anchor_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10266">
        <property type="entry name" value="CYTOCHROME C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10266:SF3">
        <property type="entry name" value="CYTOCHROME C1, HEME PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02167">
        <property type="entry name" value="Cytochrom_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00603">
        <property type="entry name" value="CYTOCHROMEC1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46626">
        <property type="entry name" value="Cytochrome c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81496">
        <property type="entry name" value="Cytochrome c1 subunit of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase), transmembrane anchor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51007">
        <property type="entry name" value="CYTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5 21 22" id="KW-0349">Heme</keyword>
    <keyword evidence="12 21 22" id="KW-0408">Iron</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 21 22" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Cytochrome c" evidence="23">
        <location>
            <begin position="78"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="binding site" description="covalent" evidence="21">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>heme c</name>
            <dbReference type="ChEBI" id="CHEBI:61717"/>
        </ligand>
    </feature>
    <feature type="binding site" description="covalent" evidence="21">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>heme c</name>
            <dbReference type="ChEBI" id="CHEBI:61717"/>
        </ligand>
    </feature>
    <feature type="binding site" description="covalent" evidence="21">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>heme c</name>
            <dbReference type="ChEBI" id="CHEBI:61717"/>
        </ligand>
    </feature>
    <feature type="binding site" description="covalent" evidence="21">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>heme c</name>
            <dbReference type="ChEBI" id="CHEBI:61717"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00040084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00041262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00041724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00041779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00062753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00079825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR602326-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON006936-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="296" mass="32702" checksum="BD5358AF2D443772" modified="2020-08-12" version="1">MRWITRLLFIVRPTVQNFSTSRAWTKNQKVATAAGVLVGGAGALLYALEQSVSASGTEVHPPELPWNHKGMLDSLDHASVRRGYEVYKQVCAACHSMRFIAYRNLVGVSHTEAEAKAEAEEIQVRDGPDEAGNYFMRPGKLSDYFPSPYPNEEAARAANNGAYPPDLSYIALARHGGEDYLFALLTGYCDAPAGVVLREGQYYNPYFPGGAISMAQALYNEAAEYSDGTPPTASQLAKDVSTFLVWAAEPYHDERKRMGIKSVGIIMILGALSYYIKRHKWAALKTRKISFHPKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VR83</accession>
    <name>A0A6E8VR83_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="9">HECT domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VR83"/>
    <dbReference type="EnsemblMetazoa" id="ACON006994-RA">
        <property type="protein sequence ID" value="ACON006994-PA"/>
        <property type="gene ID" value="ACON006994"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010601"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON006994"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041004"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2160.10:FF:000004">
        <property type="entry name" value="probable E3 ubiquitin-protein ligase HERC4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2410.10:FF:000003">
        <property type="entry name" value="probable E3 ubiquitin-protein ligase HERC4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058923">
        <property type="entry name" value="RCC1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051709">
        <property type="entry name" value="Ub-ligase/GTPase-reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45622:SF76">
        <property type="entry name" value="HECT AND RLD DOMAIN CONTAINING E3 UBIQUITIN LIGASE 4, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45622">
        <property type="entry name" value="UBIQUITIN-PROTEIN LIGASE E3A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25390">
        <property type="entry name" value="WD40_RLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00633">
        <property type="entry name" value="RCCNDNSATION"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00626">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="repeat" description="RCC1" evidence="7">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="7">
        <location>
            <begin position="55"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="7">
        <location>
            <begin position="108"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="7">
        <location>
            <begin position="161"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="7">
        <location>
            <begin position="214"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="7">
        <location>
            <begin position="266"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="7">
        <location>
            <begin position="319"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="domain" description="HECT" evidence="8">
        <location>
            <begin position="728"/>
            <end position="1055"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="6">
        <location>
            <position position="1023"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON006994-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1055" mass="118422" checksum="CA03200B2F948DD5" modified="2020-08-12" version="1">MAIYCWGNTAHGELGLGGIEEEQVMVPRKMDWSQADEVVRVECGTSHTLFLTKEGKMYACGNNDHGQLGHDLDTLPNKRPQRLTSLENYIITCISCGTAHSLALTNWGQVFSWGSNAVGQLGHDAENGRQPTPRMIKAIGAKHVVQIASGQYHCLALTNNGELYAWGSNSYGQLGIGTTSEKVPMPTLIQSLAGVPIAFIACGGNHSFAVSKSGAIFGWGKNTFGQLGLNDLNSRCYPTQLRTLRSLGVRYISCGDDFSVFLTNEGGVFTCGAGTFGQLGHGSCSNEIVPRKVFELMGSKITQIACGRRHTLAFVPSRGKIYGFGLGGVGQLGIGMVGNHSTPQIVRGPWFKTDFQSSDCDETQIIVNRIFSGGDQCFVSLLGGDESADFRVYDDSTQILSLSLEMGKELAKIEPEQTVELDVISSVETIFKSLACLNGSLLLPNDAHLCCTSKHPGVDMGAAEETFDQIRKIERESIRQLIRDSIESDLLGALTASPADVETLRIYLLLPLYHEFINPKHYQKLHAPFSKAVLQLAKNAYEIVVGWWANQSREYFERMVENCKSVVLYIINFKFPRVESPAGAEKPHQQELRYDPHLAAMLDMLAVLYWINHQKRTQKLPYEQFHINEIDDLIDIRQDYFRWTTDAKGVTFSLCNYPFVFNASAKMLLLQTDQAMQMYQAMQSAATASWLSMLYSPAANSAACQYIVLNVTRENIVQDTIRELSHYAASDLKKPIKIKFCGEEAEDAGGVRKEFFMLLLRDILDPKYGMFKSFEESNAIWFTEDYFDSDDSMFALIGILCGLAIYNFTIIALPFPLALYKKLLGEEVDMKDLQDLAPTVARSMQSILDYEGGDLEEVFDLCFTTTRDYFGEIQTIPLKPNGENIRLTQGNKQEFVNLYIDYVFNKAVEKSFRQFHAGFMRVCGGRVMKLFKAHELMSVVVGNEEYDWNVLEENAEYKNGYTCGDQTIRWFWEVFHELPLEEKKKFLLFLTGCDRIPIMGMKAIKIFIQPTPDDKFLPVAHTCFNLLDLPQYQTKEKLKYKLLQAIQQTQGFSLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VR87</accession>
    <name>A0A6E8VR87_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Gustatory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Gustatory receptor which mediates acceptance or avoidance behavior, depending on its substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the insect chemoreceptor superfamily. Gustatory receptor (GR) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VR87"/>
    <dbReference type="EnsemblMetazoa" id="ACON006877-RB">
        <property type="protein sequence ID" value="ACON006877-PB"/>
        <property type="gene ID" value="ACON006877"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACON006877"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007635">
        <property type="term" value="P:chemosensory behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008049">
        <property type="term" value="P:male courtship behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050909">
        <property type="term" value="P:sensory perception of taste"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013604">
        <property type="entry name" value="7TM_chemorcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21143:SF134">
        <property type="entry name" value="GUSTATORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21143">
        <property type="entry name" value="INVERTEBRATE GUSTATORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08395">
        <property type="entry name" value="7tm_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="55"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="90"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="155"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="189"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="280"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="320"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="393"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON006877-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="416" mass="47653" checksum="DE69D04E329515ED" modified="2020-08-12" version="1">MAWNDTEIDRLRHQLRQNISPTMRLSQCLSLAPYPLSAFQRNCSTRSVRIRVIICFRYAFATCITVAVVASQFTMFYYFPHIMYQPKVPIFIVILYYIVSILQTLTTGNMMIGCEQRRAEYEGYFEEVLHLMKETAHQPDCKTTIWYRNVTKVLLALYCIASLTVPIVMTTILWDIATIPYVMAQTVPFVVSSLILNQYFCVFVHLTSILRKMNERLARFLNMLPGSATEPPVQLRGKPLIYNVLGESLKLESSRLDQLEQLRLLHVRTVQTAGSLSEKFGIVIILIVIAAFASVNIELLEFYQSIKLGTLTPTTIFMKFLYAASKFSFYILIAYPNRLIQQENQKALFMLYRIKRISCSVELNEAIEHFISQISNLHDVHQACGMLNLDMKLISNAVAAITSIMVVLIQFSDTGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VR99</accession>
    <name>A0A6E8VR99_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DEP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="EnsemblMetazoa" id="ACON007010-RC">
        <property type="protein sequence ID" value="ACON007010-PC"/>
        <property type="gene ID" value="ACON007010"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004583"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007010"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_043233"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990130">
        <property type="term" value="C:GATOR1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904262">
        <property type="term" value="P:negative regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010508">
        <property type="term" value="P:positive regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04449">
        <property type="entry name" value="DEP_DEPDC5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045838">
        <property type="entry name" value="DEPDC5_CTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027244">
        <property type="entry name" value="IML1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055213">
        <property type="entry name" value="IML1_double_psi_beta_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048255">
        <property type="entry name" value="IML1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13179">
        <property type="entry name" value="DEP DOMAIN CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13179:SF8">
        <property type="entry name" value="GATOR COMPLEX PROTEIN DEPDC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19418">
        <property type="entry name" value="DEPDC5_CTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12257">
        <property type="entry name" value="IML1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23013">
        <property type="entry name" value="IML1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IML1 N-terminal double psi beta-barrel" evidence="4">
        <location>
            <begin position="14"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="Vacuolar membrane-associated protein Iml1 N-terminal" evidence="2">
        <location>
            <begin position="112"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="domain" description="DEPDC5 C-terminal" evidence="3">
        <location>
            <begin position="1255"/>
            <end position="1584"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="444"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="561"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1063"/>
            <end position="1105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="561"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1081"/>
            <end position="1096"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF19418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007010-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1606" mass="181836" checksum="9EA0561DBB56E0A9" modified="2020-08-12" version="1">VHTTVLLCAKMMQKLFKLVTHQKTFSSKYHSEDLVLNPKEHPDLKLNDIVEIYLPEDEGCRLLLQVTCLNKDFNSRETISVEVNVATTFNLKAYNDVYMRVVDPADVALDSVEITFKDQYIGRSEMWRLKTRLTNTCVYLNKKIESCEGCIRCQVYEMWSQGERVACGVINEDTKVVFRSNTSMVYLFLQMSSEMWDFDIYGDLYFEKAVNGFLADLYKKWQKLGSNHEVTIVLFSRTFYSAKSLDEFPPHMRDCLQIDYRGRFYEDFYRVAIQNERCDDWSTTLVQLRRLFTGYRDVVLKYHERTDLPAGVRIPPATNSTAAQGNFLEVLNISLNVFEKHYLDRSFDRTGQLSVVITPGVGVFEVDRELTNITKQRIIDNGVGSDLVCVGEQPLHAVPLLKFHNKDTLTSIDDYSMPHWINLSFYSTNKKLPYSTFIPRIKPPPAIGGSTEPQQARPPGTGRLRLSKQAADKKNEYIHNCLFDYDAYDDQIFQLPAVHGTSSLQRIARTKKTSVPSLDGFGTYARACEWEQDTLSPSRLRRKMSDPDIHHASNSLLNVSVASRSSSPNTSDILGMPKPTAITSPIMEGRTGAFGSSSGPVVRTGRALINPFDPSHVTIKLTSNRRRWTHIFPKGPQGVLIQQHHYQAVPAANPTPSYFVDLDSSSEYAHTSTCTWGRARQGSTASFDSRLNEDDLKWISENGCNRKKSVLHSPAGPHVPVTPSKSLTLLWGATGEQEWTPALTTVGRVIIGVDWKSLTMPACLPITTDYFPDKRSLHNDYVVSDYTLLPEDFNADYAQNRAVYKKPLSTDEVFKELVSQRLAQGFQLIVLPDKSVTNPPHAPCCGGHLQTPSYFSSASSVLRRQPPQEATKEYLLSIGRIFHRITLSGSAITVTRYRPRHPYPPINVDYRYRFHAPQHDTYEVSGVHFTTEKLENFNWNYMDQYICTRGDTDYPLHENMKYWRYRMYLLPKDDPAMKKILDGTVRRCDIYHEDSPFPPERQICNDFVRFVEAHLNKIKKLQPAKKARVTAPQPFRDRVFSNRLPEKSRFSILTRTGSKVLDKSGSAVQAPSPVGTGAIGTGTATSNGTTHATGNGPATPNTPGIPSPMMMNVSSVGGRTTVDGIPLSTLNTAGTEQGLLEDGDDFGGEGGKLKPTATLQEIFDALRHPAHGVGFLAPAQSMPSCTFVSYDAINWLQNRIEGPCKPVEILEEMRRNRLICHASGDFNKPVLPGFCLYYIVRQDRDSPEYVPPLGDLAAFENEWMEVEIRHPDLTRPVDPHDRGVPTFLRESIARPDEDDDDGQLLYKQTHLEIDVGSKSDRTEWGHARYHQRMVPGHAYEIVVQWITASGPIVYDLIYGWCRKAQPCGFQLVTFPADPLAEPFTEKSDPLRGPIFVPLDIDCLKQHRSCLFQEFPKETWPTRLLLFQEAIVRRFGFMRCTVETKTGSNQVSLDHQYVHCSGNMFLQVPNPNVGGMRSTRQRLASGGSNFKKPIAFINRYSASYESQMVAAPSGHEPSYITRHVTGAKGSAKDEGGEVMRRTGFLWSWNHMIPNKKWKSLVIAQTDDLFQLKMLRDFKEFCANADQRLVSFWESCWELKEKASLERT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VSZ4</accession>
    <name>A0A6E8VSZ4_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="5">PID domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VSZ4"/>
    <dbReference type="EnsemblMetazoa" id="ACON007627-RB">
        <property type="protein sequence ID" value="ACON007627-PB"/>
        <property type="gene ID" value="ACON007627"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011073"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007627"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030913"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050998">
        <property type="term" value="F:nitric-oxide synthase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01270">
        <property type="entry name" value="PTB_CAPON-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000124">
        <property type="entry name" value="carboxyl-terminal PDZ ligand of neuronal nitric oxide synthase protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051133">
        <property type="entry name" value="Adapter_Engulfment-Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006020">
        <property type="entry name" value="PTB/PI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11232:SF17">
        <property type="entry name" value="CAPON-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11232">
        <property type="entry name" value="PHOSPHOTYROSINE INTERACTION DOMAIN-CONTAINING FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00640">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00462">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01179">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PID" evidence="4">
        <location>
            <begin position="105"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="196"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="233"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="278"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="233"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="263"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007627-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="457" mass="51418" checksum="FE5A4AF37376C031" modified="2020-08-12" version="1">MPSQPYDLVKDDQDIRVPLYPEQAFYGNGLTFQAKLIGWQVVPRPSNRTEIVQAMRRIRYECKVQNSKKKKVTVSISVEGVRVCLKRKRRKKKQHQWNDPLEIELLSHPIYRIFYVSHDSNDLKIFSYIARDGSSDVFKCCVFKTNKKSQAMRIVRTVGQAFEVCHKLTIQDSGDNLGDDHSELCDVSEQDRCSDRISEDDEIKKDPAIAAHHSDSPRLTRPDHLELALLQAPASPSSPAATSSTSLQRSSTAQEKEPPEVGSSSLAKSSPLSASREMTNLRDQLDQQQQQTRQVLAQLMLVREQLISETNARMEAQARIQQLLQQNRELLEHIASLGGYHEPDRPGLSATAIGIAPQLSSTAKVARWFHTLQWTNQTQSLSRPESGFVSGDSRSERNQKSDVDMVDLLAKEHSAGAGVDLCDEYLLVEGTSNLWSKLSVKKRKKLLGLKLGKVTTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VUL9</accession>
    <name>A0A6E8VUL9_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="9">EGF-like domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CRELD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VUL9"/>
    <dbReference type="EnsemblMetazoa" id="ACON007968-RA">
        <property type="protein sequence ID" value="ACON007968-PA"/>
        <property type="gene ID" value="ACON007968"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004058"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007968"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031746"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.220.10">
        <property type="entry name" value="Hormone Receptor, Insulin-like Growth Factor Receptor 1, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021852">
        <property type="entry name" value="DUF3456"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002049">
        <property type="entry name" value="LE_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11938">
        <property type="entry name" value="DUF3456"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01248">
        <property type="entry name" value="EGF_LAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="4 5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2 5" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026213959" evidence="7">
        <location>
            <begin position="24"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="8">
        <location>
            <begin position="158"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="311"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="185"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007968-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="353" mass="38322" checksum="0817FBF1C777E940" modified="2020-08-12" version="1">MRHSRAIVASLLLVALLPTLSLGDIPKPPSLSAGGGTKKDKLKTEKLPPCKACTVLVNSFRDGIKRTERAKHDGGDAAWEEERLGSYKTSELRLVEIQERLCTDIVRGEDQCHQLAEDYETQIEEWWKNHQTTQPDLHRWLCVEQAAVCCPDGFYGPNCDPCPTCFGNGKCKGNGTRKGNGKCACDEGYTGDNCDSCSEEHYQAFRDETKLLCSRCHKACAAGGCTGAGPNSCRVCRSGWVMDSARGGCVDVDECIKEAPCTKKQFCVNNEGSYSCLECDKSCDGCNGDGPDLCEKCAEGYELRDGMCTASSDDTGVPEQQMDPSSISSTREGEPHPTELSDGEDPAAPKDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VVB7</accession>
    <name>A0A6E8VVB7_ANOCL</name>
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        <recommendedName>
            <fullName evidence="6">Reticulon-like protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Endoplasmic reticulum membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_040233095.1">
        <property type="nucleotide sequence ID" value="XM_040377161.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VVB7"/>
    <dbReference type="EnsemblMetazoa" id="ACON007940-RC">
        <property type="protein sequence ID" value="ACON007940-PC"/>
        <property type="gene ID" value="ACON007940"/>
    </dbReference>
    <dbReference type="GeneID" id="120955923"/>
    <dbReference type="CTD" id="33721"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010942"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007940"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042983"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.2480:FF:000001">
        <property type="entry name" value="Reticulon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.2480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003388">
        <property type="entry name" value="Reticulon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046964">
        <property type="entry name" value="RTN1-4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45799">
        <property type="entry name" value="RETICULON-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45799:SF2">
        <property type="entry name" value="RETICULON-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02453">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RETICULON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0256">Endoplasmic reticulum</keyword>
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    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="40"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="146"/>
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            <begin position="27"/>
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        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007940-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="218" mass="24472" checksum="B67E9661D70031EC" modified="2020-08-12" version="1">MVKRQNSRVSNGNGHYNRLDLPPRGPVESLIYWRDVKKSAVVFGGGLAILLAMSLFSLISVFSYLSLLLLFGTISFRIYKNVLQAVQKTSEGHPFKDYLELDLTLSQEKVQQLATVAVAHVNALVTGLRRLFLVEDLVDSIKFGVLLYWLTYVGALFNGMTCVMIGFVALFTLPKVYENNKQSIDTHLEMVRSKIQEITEKVKAAVPLGKKTETEKTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VVB8</accession>
    <name>A0A6E8VVB8_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
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            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VVB8"/>
    <dbReference type="EnsemblMetazoa" id="ACON008443-RC">
        <property type="protein sequence ID" value="ACON008443-PC"/>
        <property type="gene ID" value="ACON008443"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_008820"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008443"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031299"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="218"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="301"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="139"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="218"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="246"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="271"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008443-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="410" mass="47698" checksum="4413438E31A7D759" modified="2020-08-12" version="1">MKKANGPKTQLEKMMLEEDFTFCECNFAVKPTRRQDVNLPRVTAQLEKLMGTNVVRLTDRKYMQELQRRIQEDYNVTLEKRISEREAKEIERERNLILSGAGDSIPEEMTSSVFLVNQKTNVNILNKREKLKTIREKNAERLIKQGHRWERERQQMEEQSREREIERRTKREALLEQEKLYMIEENDRSLNLLRMKTKKGPSRDMNLRSMLMQQREELGRTIQKRDFDMKDERSGLGMEMEGGKGDRRRHRRGARKQRPMQRQNEVHEYQESSSPEEGYISMNNADTPEDLETSIAIKVGKGASKDSGDESEAEQDSDQAAVDEALVEQPNDANAGDDEEVAAEVDALEQQLEQQELTEAELEAAEEARLREELDKTLMEKKKARQVRNEDPKITKLKEADVSGRVGVSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VWQ2</accession>
    <name>A0A6E8VWQ2_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ITG-containing peptide</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_040234039.1">
        <property type="nucleotide sequence ID" value="XM_040378105.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VWQ2"/>
    <dbReference type="EnsemblMetazoa" id="ACON008993-RB">
        <property type="protein sequence ID" value="ACON008993-PB"/>
        <property type="gene ID" value="ACON008993"/>
    </dbReference>
    <dbReference type="GeneID" id="120956559"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_008406"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008993"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039405"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026013535" evidence="1">
        <location>
            <begin position="23"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008993-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="212" mass="23469" checksum="200A6C3BCC39620A" modified="2020-08-12" version="1">MKSPALWMAIGAILLLGGSVNAWGGLFNRFSPEMLANMGYGSHGGSYRPQSFLQNEILDEEQQSPRYEPDPCYPRACSANEYCCPGFICVAVDDVRGMCMPLYGRKYGELCRHDSDCESGLVCDISALSGASVCRPPTIVAKQYGEDCITSSDCDITRGLCCQVQRRHRQVPRKVCSYFKDPLLCVGTVAADQVKHQIEHTAGEKRIIYNKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VX09</accession>
    <name>A0A6E8VX09_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="1">DUF1279 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VX09"/>
    <dbReference type="EnsemblMetazoa" id="ACON008432-RC">
        <property type="protein sequence ID" value="ACON008432-PC"/>
        <property type="gene ID" value="ACON008432"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_008995"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008432"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034532"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008432-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="198" mass="21287" checksum="D3FBB3CCB938607B" modified="2020-08-12" version="1">MAQQVQHVFKATLAKTAGQRAMSTGIIGTLDGGSAAKKTATQSSGLHMSVQRSYPLAAALPLPPNIDASETRRFSPLRSRDINATALLTHQSYAPNAGLIDVTVDMSNGGQQGAVDPHVESYDCTGAVSLNSSMQSNVPSPFGGMHKFTHLNMPGGAWAQESKFLSHELNSSHYTNLRQEVRSDWSSYEDRPIKTEAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VX12</accession>
    <name>A0A6E8VX12_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Lipase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VX12"/>
    <dbReference type="EnsemblMetazoa" id="ACON009104-RA">
        <property type="protein sequence ID" value="ACON009104-PA"/>
        <property type="gene ID" value="ACON009104"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000234"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON009104"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041429"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016298">
        <property type="term" value="F:lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017171">
        <property type="term" value="F:serine hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00707">
        <property type="entry name" value="Pancreat_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000122">
        <property type="entry name" value="Vitellogenin-3-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013818">
        <property type="entry name" value="Lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033906">
        <property type="entry name" value="Lipase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000734">
        <property type="entry name" value="TAG_lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610:SF37">
        <property type="entry name" value="GH01208P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610">
        <property type="entry name" value="LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00151">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TAGLIPASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Lipase" evidence="5">
        <location>
            <begin position="23"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009104-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="319" mass="35641" checksum="2BD44A4352C639ED" modified="2020-08-12" version="1">MKSLKNPRVWVGMKPVRAHLDTAKFVLFYGPKFEDHETYSLEKAEDILQHSRFDKSKKTVMYFHGYIESMEVESVHVIADAYLKRGDHNIIILDWAQLADGNYLLEAVPNCKKLGSYLGSVVLRMINAGLNVDKLHLVGHSLGGQLAGYVGRTVIAQSEKRVKLNRISALDPAFPPFYPGIFATALSSKDAEFVDVIHTDAWLYGAPVSTGTADFWPNNGKTLQPGCPKRNYKLLTDNDLCSHRRSWWFWAESVAESNVASFHSVKCKSWDDFKDGKVDRAAPIVHMGIDCSSDAKGDYYLQTNGAPPYSKGVSGSKFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VX25</accession>
    <name>A0A6E8VX25_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">SRCR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON009143-RA">
        <property type="protein sequence ID" value="ACON009143-PA"/>
        <property type="gene ID" value="ACON009143"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005052"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON009143"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040058"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045217">
        <property type="term" value="P:cell-cell junction maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000016">
        <property type="entry name" value="Scavenger receptor cysteine-rich protein type 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039448">
        <property type="entry name" value="Beta_helix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053243">
        <property type="entry name" value="SJ_maturation_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47653:SF1">
        <property type="entry name" value="DELETED IN MALIGNANT BRAIN TUMORS 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47653">
        <property type="entry name" value="PROTEIN BARK BEETLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13229">
        <property type="entry name" value="Beta_helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00530">
        <property type="entry name" value="SRCR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00258">
        <property type="entry name" value="SPERACTRCPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00420">
        <property type="entry name" value="SRCR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="2712"/>
            <end position="2734"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="12">
        <location>
            <begin position="202"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="12">
        <location>
            <begin position="1083"/>
            <end position="1191"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="12">
        <location>
            <begin position="1916"/>
            <end position="2040"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="3020"/>
            <end position="3137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="3062"/>
            <end position="3079"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="3125"/>
            <end position="3137"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="273"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="1156"/>
            <end position="1166"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="2003"/>
            <end position="2013"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009143-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="3137" mass="352949" checksum="CEDDF9E82D764ABD" modified="2020-08-12" version="1">MKSHSRTRTVKQWKNRLKYRSAMMIGKLLPVLLVVLYASVLVLPGQCQVEDVDDFGDQLHHPDRDVEHVPAPSSKEIITNHIVTDHSDGEGMGVGDSHYQDGENGVDGPGGRYTEIGGDVVLGEKILRASESPYSMRTDLEVERRARLIIEAGVTIHFAPMVGITVRGSVVAMGNAENKITLTTMPNTGYKDVPSDPREAGVRLVDGPSPLAGRLQLLNNGKWRSVCSNSKNWTIADYETTCRQLGFKGGRFWNWMDRIANYEPRLLYEEPRCLGTEGSLFDCAWPSRQVGSGVCDYHSDIGVQCLPLFDQVTPHWRGLKFENAESKETLSYNHVLYDSISLSQLRHVDIVRAGTGRGGSAEAAISVMGVPPVLEFVTIDHSSYTGINVTRHDAAFSFRNVTVRRSRGFGIFMNSSYGSAHLEGVTVAENGADGIRYVGHDLRADERTDRSKVFDFCTLTSTAWQIYPLQLSFEQSPFALSAKECARSFETAYGYVLTMHFVYFELARNESAQISVYDGMSESDRLLASWDVRNQTRPQSVTSTREKLFIKLRAEPRSAVLAHFRVTSGVTKAYDLNVTQGTIVDNGGRGIAVDNLRSQVHVHETTISNNRHAAGLHVTSGAGDVNVTHSTINYNQGDGINITYYGGNRNVSRSTIESNRGHGFAVWLNQTTKERREFLEFNQTTTIEYSHIVRNLEIGVLHGNFCGDSWVNVTGNLFNDSVSDAVDIQSCWFETPDGRRMRLQIGHNNFEHSQRIAVVLNPILNLDGRIEFNHFTRGRYGALLTRNKPLEEFRPLPVRLIVQHNHFMNNEGIYVASLGLSPNTDSKTQWMLFTRNFVKGNRVKEAFGPLEDEGEGLGGEGRLNPRSRVAAPVVISSNNVDVFRNIISNQESRYEVGSQLSDQSKALNVTYNWLGNQDEEKIYDRLFHRKDRYDLAKIEYFPYLLHHSNPGTQTIAQYQKFVPFFHKEGSERIGGEVDGQEILPAGSYTVERDINVRPGGKLILNSGVILNFAPSVGMMVTGKLEARGRAPDDILFTLKREAVMLENETTEAIVDDQEFAMDIETESIVEKVPHEPQTPKVPVRLVGGANDHEGRLQVYTEGVWGTVCDYGWTITNAALVCHQLGLALNPMDWRLQRSEVPGAGTTEDVLLSNVRCTEHDIDITKCRAERASQGDFENSCGHENDVGVRCYEGAWAGLRFGVLAERADLQYVTIEKAGLFDYVTNTFKPALQMDLARHNLDSVRVVENLQDGLGIIYSDIYAGSSVNNVKNSEFSANRGNGISIKQLGLKVQGSIIKDNRGSGINHDQVVSSLEQREITSWFNMVPDFNVDDSDYRPILVPEGGTTNIDIDQWQIKHLITTPVRTSEPVERNIIIQCQPGYVIGVQLLNPIENRSTENIWIHDSLTGSTESDIWQVKRDLSVFPLTTSSYGAILQYRSGTNALGGAVLVLRSIQAPIQNIYNRIVRGPVPTLQITSTKIQRNFRGITGTYYNRYLGESGELYLRKANESIKLINCEISHNREEAVFIHSPFWDVHVSNISEITVHINNSLVRDNGRGIRQFSKDLRSSNNLFHYVLQDTTVESNSYGGLELSLPYVWQYNENFTHSVFLGNDTWARNRKFGILIDGHYAAVNISSNIFTENVCAQGVIGFRGMEKKMRIDNNRITRNYGSYMVEFRSDSQSEILGEIPALLAFNQIESNEELKDTRSSMRNFIRGERNNANDPTCVIGFGGVQKVQIYRNVISNNQQDYDLIAGVKSARLRNYLDARENWWGSADELHIRRRIFDFDDWNNHAEALYRPYLIEDIIDGSVSVSEGPSPPVDLNALGGRILEDLSIFRRELPYVIRSDITVMPGVTLSIFPGVVMEFAPNVGILVLGTLLARGTSDAEIVMKPIESASEGMHRVERSLENMVNYDSIRLCTNRNCSQGEGESERAKEGFLEYYNHTTLQWIPICDRRFTERNAQVVCRELGYDPLDVFFGHDRRIEFHTNSLTRIWSWVEPMECHGDELRLEECPERLNGQLYGRRHECQWDSEFVFISCNGESEQRNYWGGVRFANQDFEASMYEHRLHDTHTHTTARPVESVMDFVRIQRAGLLHGEKSPAIQTIAKNPSISSVSIRDSAHHGVNLISPTNAIHLNHLQILRSLGQGINAISLTGEGRESDESSYSPLKDLDLPYNLFSLVDICDTNKEITLEERVLVYYKYDNNPVNCVKIFKSAYRVKPLGFRLLQSNLFNHSKEYGRRDMIQLMDGDIYNVSARIIGTVDADSDNQKRLFRTREPALSVRLIASGAPARHGFIAEVVTLPISAIGFNRDAQHNISNTEIQGCVGGALQYTSVGEVSPILTLERNRIMNNCRQLYGNFSSCESAIRIDVQNMQSLHFRNNLIQANQGGLSIRADSRGSATSLRGWIHHNLFARNRNRPAIYVDGRQSSPYQEVIIHNNYITQNDATFRDVVVLRQVVSNFTYNYVHRNRGLRIVQVSGFDRVRLPIYQTTTHNGFYDNVATDWEGRATIVAGTAGQHYVDNIFANPDNDYEIITVNRSITLDVWKTKIDARHNYWSYNETLAVSSRIRDRFDDPQLLEVSFLPLHMNNLTVLDGKCPPGWTLLIDTCYMYVGAPMSFREARDFCRSDNASLPFISGDANALWMFIEQQSRYLRNYERVWVQDANYIDRCTSFLYQSVEVEECHNRHAFLCEIDPKVEIDPLHWTASIEVIGIIVALALVIVLICIVCVCWCQKSRYRQAQRLQRRNSIRQSMRSLNSIDPQGSLRRRNYAMSRSTDTLRTATTNDYKRMASNGSIESVDKSVLSTETSYDVYDQKQKQQYSNEYAEQLKSQLGYSESNGAPSEYIQASQANGAAPAKTKVYGLPEYSSHGGSIALELAFRNEGFRDTSTTYSGVTRQNSLSTAINEESPIIHAPGGEEEMLEETGSDYYGNSSTLPMRVQDKLSFLHELKQHLPEYEPPTASVSHSSFQPARSSQTESLVGGVNGAAIPYSVQRSPPARPAPPVVPPMGHVYQQPSIQVRRPAPPEPEQMRRPDSYIKAVKKYATPGRERDSILPPPRTDSQQQQRPKTLYESSGEQLDPAPSMPPAGGSRSNYARSKSEALLETNFDGTPGVDGQPVLSADSRSYSQPLETAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VXD1</accession>
    <name>A0A6E8VXD1_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Gustatory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Gustatory receptor which mediates acceptance or avoidance behavior, depending on its substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the insect chemoreceptor superfamily. Gustatory receptor (GR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VXD1"/>
    <dbReference type="EnsemblMetazoa" id="ACON009256-RA">
        <property type="protein sequence ID" value="ACON009256-PA"/>
        <property type="gene ID" value="ACON009256"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007990"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON009256"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007635">
        <property type="term" value="P:chemosensory behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008049">
        <property type="term" value="P:male courtship behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050909">
        <property type="term" value="P:sensory perception of taste"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013604">
        <property type="entry name" value="7TM_chemorcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21143:SF133">
        <property type="entry name" value="GUSTATORY AND PHEROMONE RECEPTOR 32A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21143">
        <property type="entry name" value="INVERTEBRATE GUSTATORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08395">
        <property type="entry name" value="7tm_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="70"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="245"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="276"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="357"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009256-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="383" mass="44508" checksum="EA5DFF4A20DEA852" modified="2020-08-12" version="1">MKTLQVLSRLLHLKTVLLGVVPFHFHRSTLTLELRRELLLYCCAIVILGPTGRFYIYLTTFLRMRGKFIVAFYSALFIELSDVVLLIVPPCCLLYRHKQVKRLFVLLVKLYHNPAVSAFPFRRWFRTCLLFNLMYESAYGVNFLLNIFQPGSTFHLEIVNLLLSTALSQLSKSAVMLIMYGSGQYLQMALVQLKEHLLLGVHRSTGPVQQETLRQLHVRPAGWMQYYGQLWQVCEILNDLFGVPLITYCLMTLMHLTFICYVMLTKVMVEWHNFSVVQNVLAGVELMWTVFELLCLMRIVSTFARTREESLKTQKLLLRLNLSPMDHKLKQSIEVFALQTLHQPIEFTACRMFTLDYTVLFSIAAAVTNYLIILIQFEMAIEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VXF1</accession>
    <name>A0A6E8VXF1_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Gustatory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Gustatory receptor which mediates acceptance or avoidance behavior, depending on its substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the insect chemoreceptor superfamily. Gustatory receptor (GR) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VXF1"/>
    <dbReference type="EnsemblMetazoa" id="ACON009256-RC">
        <property type="protein sequence ID" value="ACON009256-PC"/>
        <property type="gene ID" value="ACON009256"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007990"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON009256"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007635">
        <property type="term" value="P:chemosensory behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008049">
        <property type="term" value="P:male courtship behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050909">
        <property type="term" value="P:sensory perception of taste"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013604">
        <property type="entry name" value="7TM_chemorcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21143:SF133">
        <property type="entry name" value="GUSTATORY AND PHEROMONE RECEPTOR 32A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21143">
        <property type="entry name" value="INVERTEBRATE GUSTATORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08395">
        <property type="entry name" value="7tm_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="90"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="123"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="182"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="265"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="296"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="377"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009256-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="403" mass="46406" checksum="DFF90FED0351C710" modified="2020-08-12" version="1">MNRAEQDRRYSCKQRTSSRTMPLLELLARLLHLKMVALGVVPYEFHPATLSLQFRRPLLLYCFIIAIIGPMGRCWVYLTTFFTMNFGVSVAHYTAMFMELLDAILLVVPPCYLLCRHKKVKELFVLLVKVYYSSLSLSIFPLRLWFRACFLFNLVYDSAYGVNVALNLVLPGTGIHEKYVKILSWSVLSQLTKSIVLLIIYGSAQYLLRMVDHLKDHLAAGRLSTNPVYQEQIRKQHHGPAGYLEFYEQLGKLSETLNDLYGVPLITYFLMTLIHLTFVCYMTLTKVLTKWSYLSWHALLIAGIAVASNVIDLLCLMKIVGMFARTREESLKTQKLLLRLNLSPMDHKLKQSIEVFALQTLHQPIEFTACRMFTLDYTVLFSIAAAVTNYLIILIQFEMAIEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VXF8</accession>
    <name>A0A6E8VXF8_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Semaphorin-1A</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Semaphorin-I</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the semaphorin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VXF8"/>
    <dbReference type="EnsemblMetazoa" id="ACON008656-RA">
        <property type="protein sequence ID" value="ACON008656-PA"/>
        <property type="gene ID" value="ACON008656"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005742"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008656"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030955"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045499">
        <property type="term" value="F:chemorepellent activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030215">
        <property type="term" value="F:semaphorin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030335">
        <property type="term" value="P:positive regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071526">
        <property type="term" value="P:semaphorin-plexin signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11237">
        <property type="entry name" value="Sema_1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1680.10:FF:000016">
        <property type="entry name" value="Putative Semaphorin-6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000346">
        <property type="entry name" value="Sema-1a, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1680.10">
        <property type="entry name" value="ligand-binding face of the semaphorins, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002165">
        <property type="entry name" value="Plexin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016201">
        <property type="entry name" value="PSI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042068">
        <property type="entry name" value="SEM1A_sema_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001627">
        <property type="entry name" value="Semap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036352">
        <property type="entry name" value="Semap_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027231">
        <property type="entry name" value="Semaphorin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036">
        <property type="entry name" value="SEMAPHORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036:SF127">
        <property type="entry name" value="SEMAPHORIN-1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01437">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01403">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00423">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00630">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103575">
        <property type="entry name" value="Plexin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101912">
        <property type="entry name" value="Sema domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51004">
        <property type="entry name" value="SEMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5" id="KW-0221">Differentiation</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="645"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="domain" description="Sema" evidence="16">
        <location>
            <begin position="53"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="721"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="721"/>
            <end position="734"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00074143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00083066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008656-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="782" mass="87758" checksum="968E4541F4303877" modified="2020-08-12" version="1">MSAESIQSSAAHGRQGRRSVPVARGGPLLVGYLLATVFVVGTLCAWQENVRPKLYVTLADKEYLRFQGNESTNDFFKLVLRDPNSNNLLVGARNIVYNLSIYDLAETTRLSWYSSDDDIKMCIVKGKDEDLCQNYIRVLAIPAQGSLLSCGTNAFRPLCRTYSINGNNYTVETEKPGQAMCPYDPTHNSTAVFVDNELYSGTVADFSGLDPIIYREHLQTEQYDSLSLNAPNFVGSFTQGDFVYFFFRETAVEFINCGKAIFSRVARVCKWDKGGPNRLKNRWTSYLKSRLNCSMPGDFPFYFDEIQSTSNLVEGRYGHANSKLIYGVFSTAPNAIAGSAICAFSLQEISDTFEGNFKEQSGLNSNWLPVNPLNVPDPRPGSCHNDSRTLPDLTLNFKKTHSLMDETVPAFFGSPILTRVSTMYRFTAIAVDPQIKTPGGKTYDVIFVGTDHGKILKTVNAESADSNKKVTSVVIEEIDALQTNEPIRTLEIVRTTQYDTPKDGSYDDGKLIIVTDTTVLAIKLHRCSSEKITSCSECVALQDPYCAWDKIVGKCRSHGAPRWSEENVFYQSVATGDHAACPSGKMAKDANVVERQGYRDLEYDPQRPFRDQPADKIQFNSENYNDKGPQITPDLINAQYTVETLVMAVLAGAIFALLVGFITGYFCGRRCHKDEDDNLPYPDTEYEYFEQRQNVNRIQAEPKLLPQVEEVTYAEPVLLPQPSSQNKMQHSPKNTLRKPMGHHHGVNSETLFQFQPDNYNNARDNYRGRDNFGTLRSHQVYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VYU3</accession>
    <name>A0A6E8VYU3_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Gustatory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Gustatory receptor which mediates acceptance or avoidance behavior, depending on its substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the insect chemoreceptor superfamily. Gustatory receptor (GR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VYU3"/>
    <dbReference type="EnsemblMetazoa" id="ACON009805-RA">
        <property type="protein sequence ID" value="ACON009805-PA"/>
        <property type="gene ID" value="ACON009805"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACON009805"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034541"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007635">
        <property type="term" value="P:chemosensory behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008049">
        <property type="term" value="P:male courtship behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050909">
        <property type="term" value="P:sensory perception of taste"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013604">
        <property type="entry name" value="7TM_chemorcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21143:SF134">
        <property type="entry name" value="GUSTATORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21143">
        <property type="entry name" value="INVERTEBRATE GUSTATORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08395">
        <property type="entry name" value="7tm_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="39"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="71"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="134"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="157"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="225"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="345"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009805-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="388" mass="44964" checksum="830184D5E5880516" modified="2020-08-12" version="1">MSSLYRQHSRIFQISRLLYFTPCSYNEELGRFEATKRNLAFLGAALVLTIPFWVYDIHLMATNFLHTYTTVFAAVGGIELLIYVSVVMCTILNVLVKRQRITRLMNVLFRPDRILDSCSPTNSADTRYNDNRKLVVFVLFILFGMCIKLSYFKKAEIKILSVMIAGRFLAIWVLIFVHRLHVRAIGQRMEQLRVLYASEELEQHLDYFLHLYDRYSRQIGEVDDCYSFPVVLVFLLVMLQLIYLAEHVYSTIETGTMMPVADNFFYSLFGQVWQTMYGALGYYCISACGTASEEVEETALCTRHFDDYRLQNTRAAKQIQKFLLKNLHQKKKFSACGFFDIDNTVIYMVFSSIVTYLVILIQFKQLETDLTQAGDGYNVTSNVSTVQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VYU7</accession>
    <name>A0A6E8VYU7_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Gustatory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Gustatory receptor which mediates acceptance or avoidance behavior, depending on its substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the insect chemoreceptor superfamily. Gustatory receptor (GR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VYU7"/>
    <dbReference type="EnsemblMetazoa" id="ACON009805-RF">
        <property type="protein sequence ID" value="ACON009805-PF"/>
        <property type="gene ID" value="ACON009805"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACON009805"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007635">
        <property type="term" value="P:chemosensory behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008049">
        <property type="term" value="P:male courtship behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050909">
        <property type="term" value="P:sensory perception of taste"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013604">
        <property type="entry name" value="7TM_chemorcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21143:SF134">
        <property type="entry name" value="GUSTATORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21143">
        <property type="entry name" value="INVERTEBRATE GUSTATORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08395">
        <property type="entry name" value="7tm_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="143"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="172"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="241"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="357"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009805-PF"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="400" mass="45951" checksum="026F826070A2B6E9" modified="2020-08-12" version="1">MRCIRVHLAYMGRLGLTMVKYDSEKHTFNSVQNARFKFSCLFAVALISSGYLIWRSPMDYSVYKFDSKIFDITIFIINTIITNTIYIILPLQCYCKHHQLANSLNALLINDSILRRLALLVGKEPPCYTEATYFSFIMRWDGIFFFSFFFCSYFMKNWVIDEFVHLQLSVCIYLAMDLALGWTLGLCCVVMLVGIEQLSHIIDLLLKASDERAIAPELISPILWDVYDRVTIHVRKDLSQYCGPIVVLFTSLFVLECAISLVDLGGLLYDGSDLDMSLIAVGALWLLFDFKKFIVPLLLSECLKQKVEETALCTRHFDDYRLQNTRAAKQIQKFLLKNLHQKKKFSACGFFDIDNTVIYMVFSSIVTYLVILIQFKQLETDLTQAGDGYNVTSNVSTVQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VYU8</accession>
    <name>A0A6E8VYU8_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Calponin-homology (CH) domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Nav/unc-53 family.</text>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON009424-RA">
        <property type="protein sequence ID" value="ACON009424-PA"/>
        <property type="gene ID" value="ACON009424"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002403"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON009424"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035529"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022008">
        <property type="term" value="P:neurogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001111">
        <property type="entry name" value="neuron navigator 2 isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057568">
        <property type="entry name" value="CortBP2_NAV1-like_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039041">
        <property type="entry name" value="Nav/unc-53"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057126">
        <property type="entry name" value="NAV1-like_ubiquitin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12784:SF28">
        <property type="entry name" value="PROTEIN SICKIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12784">
        <property type="entry name" value="STEERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25408">
        <property type="entry name" value="AAA_lid_NAV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23092">
        <property type="entry name" value="Ubiquitin_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="5">
        <location>
            <begin position="1106"/>
            <end position="1266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="256"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="580"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="843"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="962"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1410"/>
            <end position="1432"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="806"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="74"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="106"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="121"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="139"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="274"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="585"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="601"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="621"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="649"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="679"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="697"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="735"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="889"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1419"/>
            <end position="1432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009424-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1432" mass="154907" checksum="69F7C90B494D5A9A" modified="2020-08-12" version="1">MSMVKSQLPDGFATIRTRGLTRSKSRAEQYAPAPGQLSGRRSTMIGVVPPVMKPSESGPLFECPAGEQNHENGQELQQGQQQKQLRYKSSSATAIAMPPPVPPRTSSERTVSDDRSSSVETPEDDDVEVEVGDGEVEDGQQPTSGEQQQQPPTGPYREDDDIYYTSSLTYETFKPLTRSAANLELKVGDTTSNYLNLAQSSEYIESLAYDFSLPPLKSPQYINELNMMRTRALDVSGKGGEQVAPMPLYFAAPPPYTNGMRPDSPQSYGKIRSSYRSTTPESLDNGSSNSSTYSHPVHHGSNGSSRSAFLYDVPAKTIVAVPTLHSLQYRKSFNHSNSRTDIIYEEPKMLHRNGGGISSDTDSSYDGASSGGNGVACYLKIHKNASAELLNFADISTNMLGSRPNGYIRRYATLGHPGKDLRYGPGGAGSDNGGMCGEEDEFGAGAKSTSSLNDQDLVVKVDDDLLNYKVGCQTTLRSKPVIPWYELAIRKDHRQSCPAMKEAHVVAAFEQSLSNMTHRLQSLTATTEKKDSEILDMRQTIELLRKQSIQAGLTTAHMQSMGVQVNGQVNGTAIAAEQTKTTTTTGGGGGSGATNGGQKSTGGASSAAPAGAEMQRHHSSDSMCSLNSVSSGCSAQDKSKQKKKGWLRSSFTKAFSRNAKISKTNRHVTAGGSNGGGSTTTTAAAAAGTVPDGAAGNHHHHHHHHHHHHGSVLENGTTTTTTTTSALGLKHAAGIPPPPGAGLPPPSPTKGSPHKTMTVIDNAKPVDAIVELNGNPVVEDLKKQLREKDLVLTDIRLEALSSASQLESLKDTVMKMRQEMMNLKQNNERLQRLVTTRSLAGSEVSLGPVSPSGSIGEPRRYSLAADNGMGRAPLELPQNLDETEEDNIPPAPAPEPPPAPLSPAIIAELSPTIERAAMVNEILTTPAEDVADPVDGKKISIAVYLGQPESFPKYAEEMNECDNYYHSSPDDEEKGEAGEKGGHPRGGSFSLGSGVETNEFVIAYTYISGKTTWQNLDYIVRKTFKDYLSRIDPGTNLGLNTDSITSYHLGEAKRGPEMGFPELLPCGYIIGNVKTLYICLQGVGSLAFDSLILRNIVHRYISLLTEHRRLILCGPSGTGKSYLARKLAEFLVARAGRDNPIEAIATFNVDHKSSKELQQYLGHIAEQASMSNGAAEELPAVIILDNLHHASALGDVFSCLLSATAAKLPCIIGTMSQATCNTTNLQLHHNFRWVLTANHMEPVKGFLGRFLRRKLFSVELQNQQQQPQLEKVLKWLPTVWQHINSFLETHSSSDVTIGPRLFLSCPMNLPDSQVWFTDVWNYHLAPYLMDAVREGVQLYGRRGGAWVDPCTYIRETYPWPVGPTTVPQLRQITADDVGLEASAPGNAENQDPLLNMLMRLQEAANYNGNAEQDSDCASLDSNMTHDSSAGAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VZG0</accession>
    <name>A0A6E8VZG0_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">MABP1/WDR62 second WD40 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="EnsemblMetazoa" id="ACON009285-RA">
        <property type="protein sequence ID" value="ACON009285-PA"/>
        <property type="gene ID" value="ACON009285"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_013144"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON009285"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039100"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007099">
        <property type="term" value="P:centriole replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056162">
        <property type="entry name" value="WD40_MABP1-WDR62_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052779">
        <property type="entry name" value="WDR62"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45589">
        <property type="entry name" value="WD REPEAT DOMAIN 62, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45589:SF1">
        <property type="entry name" value="WD REPEAT DOMAIN 62, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24782">
        <property type="entry name" value="WD40_MABP1-WDR62_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="MABP1/WDR62 second WD40" evidence="3">
        <location>
            <begin position="408"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="418"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="485"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="698"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="772"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="963"/>
            <end position="1007"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1032"/>
            <end position="1117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1162"/>
            <end position="1260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1274"/>
            <end position="1314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1340"/>
            <end position="1368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1398"/>
            <end position="1417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="781"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="825"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="889"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="910"/>
            <end position="923"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1032"/>
            <end position="1043"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1067"/>
            <end position="1087"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1162"/>
            <end position="1195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1228"/>
            <end position="1249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="1302"/>
            <end position="1312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1349"/>
            <end position="1362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1406"/>
            <end position="1417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009285-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1535" mass="166050" checksum="8C9A6989C267FB4B" modified="2020-08-12" version="1">MFAPRNSSPPLQIYNSDEVAAYQIKLKKILGLTVCSSAGLDVSSTTGVLAYPAGCTVVLFNSKKLSQNFLLNTTKKAITAVAYSECGRYLATGECGHNPSIKVWELDASGGPGGGGAIENGAAGSIVAEFSGHKYAVSCVAFSPTGKYLVSVGSQHDNIVNVFDWKANLKIASNKVTAKVVAVSFSEDGNYFVTVGNRHVKYWYLEGSRKYKEPIPLMGRSAILGELRNNDFCAVACGKGEMAESTYAITRNGHLVEFNARRLLDKWVMCRTNAANCMVASTKYILVGCAEAIVRVFNAETLEYITTLPRTHFLGVDVAQGVHINHMMAVPQNAKYPDTIAIVYDEARSKVTCVYNDHSLYIWDLRDIRRVGKSQSFLYHSACIWGVETVPFSYQQQLRQHNGGAANGAGDTLPSDCFMTCSSDDTIRVWDVDSCETNEVYRKNIYSKELLKVLYIDDELNFIKDTDNPIHSTEKNSSYDGRNGVRCIKISPDSRQLATGDRSGNIRIYNLSNLKLITTIEAHDSEVLCLEYTNEKIERRLLASASRDRLIHIFDCEANYRILQTLDDHSSSITSVRFIGAGKQFQMVSCGADKSIIFRHFQNNVFLRGNNCSGKNTLYDMEVDSNSKHILTACQDRNIRVYSTQNAKHTKTFKGSHSDEGSLIKVSLDLSGIYIATSCTDKTLSVYDYYSNECMARMYGHSELVTGLKFTNDCKYLISASGDGCVFIWQVPHDMIVTMQARLSQQALRSGLQPIPRTLQPTNPFTDAILNHPQPTPPAPSSVSEFGSPPNSAFLVDDAPGTPGYRFSDVGQLPQWAKRKPSEDLQSSSPVLGSSPSQTGANFGGLAPKPRGRWGQKGVGGQLEDPFDLRNIVESSPLGTTFVGGESRQQPTTPQPSATAATGQPIHPPATNTPTSGYNSSGSKDVYSNAYLSEDSSIDSGRENRRPDLSSFLHKKITETKALNSLNSESNTEHDGDVEDISDGERTSSDHGMVYYPSAAPSTPTDFKINEIDTNELRKSIRRQKLEKQGLSLAAQFQSAASGTGTGTSDDEDEGSTPSGDNADRSLASTLGGSSESIPQQTSSTFLQAVLDGPGSLSDRERSQARKSMSAKHNNDAKITTSISKSFANNKKEELMKVINEAKAKLENVGYRSGLRASHSISDLSHSMNMSGASPNRTQRIGGNPRSLISAQDSESSIRRACSLSDLSMGKGYKSPNQSTAGSGQYKPSVSNRNTSTPTRNTPKRSTSSVGKGGASLSTGMGVRSISVGMLNQASDSEAEPAGTRGGLLKPTISSQNKINGTGNGTGPGSGGKYINPRSAAGIINRRKGLQNAYSINISTAGQDESSSEESPTSTVSTVPAKPAVPPRPRSIAFEHKRIANASQLNVKKTVTVGGTTTIPADATTNGGSNNNGSSNNGAMLPVKDADLDNADLTNQLCTNIINQLVQTTSTVIQLHQRLKSSDEASINGRNNSLMLKELENAVIMTQNMLTKVTNRNHNDGINNNSMSNNTNNQIMYEKCVDMLNQVQKHVNNNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W0R1</accession>
    <name>A0A6E8W0R1_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Gustatory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Gustatory receptor which mediates acceptance or avoidance behavior, depending on its substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the insect chemoreceptor superfamily. Gustatory receptor (GR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8W0R1"/>
    <dbReference type="EnsemblMetazoa" id="ACON009805-RC">
        <property type="protein sequence ID" value="ACON009805-PC"/>
        <property type="gene ID" value="ACON009805"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACON009805"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007635">
        <property type="term" value="P:chemosensory behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008049">
        <property type="term" value="P:male courtship behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050909">
        <property type="term" value="P:sensory perception of taste"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013604">
        <property type="entry name" value="7TM_chemorcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21143:SF134">
        <property type="entry name" value="GUSTATORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21143">
        <property type="entry name" value="INVERTEBRATE GUSTATORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08395">
        <property type="entry name" value="7tm_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="39"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="69"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="136"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="163"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="283"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="353"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009805-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="396" mass="46344" checksum="3F9F0F482E25C90D" modified="2020-08-12" version="1">MSSLVKRYQRLFAVATVMYILPCRYNFRTNMFEKSWTNMVALVLNIVLHSLCLWLDLNAIKISIGYMSFVMLGVIVIYLVTYALMLVVIIWNAFYHRDSFVHLFNSLFAKEDWLLQWVVMKGIVTKLNLQTRHNGSMGTLALLVILNSFYNFAYAANSTIFKLYWLILLRFCGMFVMVELYRACVIVIEERMKQLHSLLQLTINGPKLNVEHQVELFIERFEHYYELIECINRCFAVPLIHVLLLILLERTVAAYDVYDNYNLLDVMSVWDKFGLLFRQIWQMIYPIIICMIGITGNLTLIQVEETALCTRHFDDYRLQNTRAAKQIQKFLLKNLHQKKKFSACGFFDIDNTVIYMVFSSIVTYLVILIQFKQLETDLTQAGDGYNVTSNVSTVQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W0R4</accession>
    <name>A0A6E8W0R4_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Myosin heavy chain</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Muscle contraction.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Myofibril</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040234089.1">
        <property type="nucleotide sequence ID" value="XM_040378155.2"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON010147-RI">
        <property type="protein sequence ID" value="ACON010147-PI"/>
        <property type="gene ID" value="ACON010147"/>
    </dbReference>
    <dbReference type="GeneID" id="120956570"/>
    <dbReference type="CTD" id="35007"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009622"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON010147"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041963"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031672">
        <property type="term" value="C:A band"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032982">
        <property type="term" value="C:myosin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008307">
        <property type="term" value="F:structural constituent of muscle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007298">
        <property type="term" value="P:border follicle cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040011">
        <property type="term" value="P:locomotion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006936">
        <property type="term" value="P:muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031033">
        <property type="term" value="P:myosin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007424">
        <property type="term" value="P:open tracheal system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045214">
        <property type="term" value="P:sarcomere organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14909">
        <property type="entry name" value="MYSc_Myh1_insects_crustaceans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.820:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000036">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000008">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.530:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.360:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.4820:FF:000002">
        <property type="entry name" value="Myosin heavy chain 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000038">
        <property type="entry name" value="Myosin heavy chain muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000019">
        <property type="entry name" value="Myosin heavy chain, isoform G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000021">
        <property type="entry name" value="Myosin heavy chain, isoform G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000025">
        <property type="entry name" value="Myosin heavy chain, isoform G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000009">
        <property type="entry name" value="Myosin heavy chain, isoform G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000010">
        <property type="entry name" value="Myosin heavy chain, isoform G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000005">
        <property type="entry name" value="Myosin heavy chain, isoform I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000024">
        <property type="entry name" value="Myosin heavy chain, isoform J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.720:FF:000001">
        <property type="entry name" value="Myosin heavy chain, muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000050">
        <property type="entry name" value="Myosin heavy chain, muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.370">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.360">
        <property type="entry name" value="Myosin S1 fragment, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008989">
        <property type="entry name" value="Myosin_S1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002928">
        <property type="entry name" value="Myosin_tail"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004009">
        <property type="entry name" value="SH3_Myosin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014751">
        <property type="entry name" value="XRCC4-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615:SF27">
        <property type="entry name" value="MYOSIN HEAVY CHAIN, MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615">
        <property type="entry name" value="MYOSIN HEAVY CHAIN, NON-MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02736">
        <property type="entry name" value="Myosin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01576">
        <property type="entry name" value="Myosin_tail_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90257">
        <property type="entry name" value="Myosin rod fragments"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51844">
        <property type="entry name" value="SH3_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 14" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="6 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 14" id="KW-0505">Motor protein</keyword>
    <keyword evidence="10" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="8 14" id="KW-0518">Myosin</keyword>
    <keyword evidence="5 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0787">Thick filament</keyword>
    <feature type="domain" description="Myosin N-terminal SH3-like" evidence="17">
        <location>
            <begin position="34"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="Myosin motor" evidence="16">
        <location>
            <begin position="87"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="14">
        <location>
            <begin position="657"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1823"/>
            <end position="1842"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1920"/>
            <end position="1961"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="180"/>
            <end position="187"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010147-PI"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="20">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1961" mass="224116" checksum="A2AAA74CC6BC6C19" modified="2020-08-12" version="1">MPKPPVQVGEDPDPTEFLFVSLEQKRIDQSKPYDSKKACWVPEEKEGYVLGEIKATKGELVTVALPGGEEKNFKKEQLSQVNPPKFEKVEDMADLTYLNEAAVLHNLRQRYYSKLIYTYSGLFCVVINPYKRYPLYTNRCAKMYRGKRRNEVPPHLFAVSDGAYVNMLTNHENQSMLITGESGAGKTENTKKVIAYFATIGASGKKDENAEKKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFTGSGKLAGADIETYLLEKARVISQQTLERSYHIFYQIMSGSVKGLKEKCLLSNNIHDYHIVAQGKTTIPSVDDGEEMQITDEAFNVLGFTQEEKDNIYRITSAVMHMGRMQFKQKGREEQAEADGTEDGDRVAKLLGVGTDDLYKNLLKPRIKVGNEFVTKGQNKDQVTNSVGALCKGIFDRLFKWLVKKCNETLDTKQKRAQFIGVLDIAGFEIFDFNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYKREGINWAFIDFGMDLLACIDLIEKPMGILSILEEESMFPKATDQTFAEKLMTNHLGKSAPFMKPRPPKPGIPAGHFAIGHYAGVVSYNITGWLEKNKDPLNDTVVDQFKKGSNALMVEIFADHPGQSADPAAAKGGRGKKGAGFATVSSSYKEQLNNLMTTLKSTQPHFVRCIIPNEMKTAGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKLRYKILCPQLIKEPCSPEKVTQIVLTHIQLPEEQFRMGKTKVFFRAGVLGQMEEFRDERLSKIMSWMQAWCRGYLSRKEFKKMQEQRVSLEIVQRNLRKYLKLRTWAWWKLWQKVKPLLNVSRVEDQIAKLEEKATKAQEAYEKEEKLRKELEALNSKLLAEKTALLDSLSGEKGALQEYQEKAAKLTAQKNDLENQLRDTQERLAQEEDARNQLFQTKKKLEQEIGSQKKDAEDLELQIQKIEQDKASKDHQIRNLNDEIAHQDELINKLNKEKKMQGEVNQKTAEELQAAEDKVNHLNKVKAKLEQTLDELEDSLEREKKLRGDVEKAKRKVEGDLKLTQEAVADLERNKKELEQTVLRKDKEISALSAKLEDEQSLVGKLQKQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELAKLRRDLEEANIQHEGTLANLRKKHNDAVAEMAEQVDQLNKLKTKAEKERTQYFAELNDARIGCDQLSNEKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEDAESQVSQLSKIKISLTQQLEDTKRLADEEARERATLLGKFRNLEHDLDNLREQVEEEAEGKGDIQRQLSKANAEAQLWRSKYESEGVARAEELEEAKRKLQARLAEAEETIESLNQKCIALEKTKQRLATEVEDLQLEVDRASSIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKSRKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDVQSALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELSDAHEQLNEVSAQNASIAAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAESNALKGGKKAIQKLEQRVRELESELDSEQRRHADAQKNLRKSERRIKELTFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADIAEQAATKFRTKGGRAGSVQRGASPAPQRQPSAMPALAGLNLPTFDDHGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W0R6</accession>
    <name>A0A6E8W0R6_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Gustatory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Gustatory receptor which mediates acceptance or avoidance behavior, depending on its substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the insect chemoreceptor superfamily. Gustatory receptor (GR) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8W0R6"/>
    <dbReference type="EnsemblMetazoa" id="ACON009805-RH">
        <property type="protein sequence ID" value="ACON009805-PH"/>
        <property type="gene ID" value="ACON009805"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACON009805"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007635">
        <property type="term" value="P:chemosensory behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008049">
        <property type="term" value="P:male courtship behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050909">
        <property type="term" value="P:sensory perception of taste"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013604">
        <property type="entry name" value="7TM_chemorcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21143:SF134">
        <property type="entry name" value="GUSTATORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21143">
        <property type="entry name" value="INVERTEBRATE GUSTATORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08395">
        <property type="entry name" value="7tm_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="34"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="74"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="137"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="182"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="238"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="275"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="356"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009805-PH"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="399" mass="45721" checksum="1D4DC4A74630A788" modified="2020-08-12" version="1">MWCLRIHLQCMEKLGLTMVKYDPGKHTFISVPYAPLKFACLFTAALVSSGYLLWRSPLDYIVYKLDTRMFDITIFIINTTISCTLCLILPLQCYYKHRQLSSSLNALLKNDSILRQAILEMGGKPLCYKEATLFSRIMRWDGIVCVSFFFCSYFIKSWSKCNFVHIQLSVCIYLATDLALDWTLGLCCVMILVGIAQLSHIFDLLKIASIRRTIALERIWPPLWQVYDRVTIDVRKDLSQYYGPIVLLFSCMIGLESAVKMIDVGGALNDGTGDLTILILGCLWLLFDIKKLIVLLLISERLKQKVEETALCTRHFDDYRLQNTRAAKQIQKFLLKNLHQKKKFSACGFFDIDNTVIYMVFSSIVTYLVILIQFKQLETDLTQAGDGYNVTSNVSTVQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W0R9</accession>
    <name>A0A6E8W0R9_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Gustatory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Gustatory receptor which mediates acceptance or avoidance behavior, depending on its substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the insect chemoreceptor superfamily. Gustatory receptor (GR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8W0R9"/>
    <dbReference type="EnsemblMetazoa" id="ACON009805-RM">
        <property type="protein sequence ID" value="ACON009805-PM"/>
        <property type="gene ID" value="ACON009805"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACON009805"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007635">
        <property type="term" value="P:chemosensory behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008049">
        <property type="term" value="P:male courtship behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050909">
        <property type="term" value="P:sensory perception of taste"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013604">
        <property type="entry name" value="7TM_chemorcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21143:SF134">
        <property type="entry name" value="GUSTATORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21143">
        <property type="entry name" value="INVERTEBRATE GUSTATORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08395">
        <property type="entry name" value="7tm_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="42"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="73"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="132"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="173"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="354"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009805-PM"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="397" mass="45286" checksum="3CE97683B47B95A2" modified="2020-08-12" version="1">MHVFSNLRMLIRCLQFVGQLSIQNHAEEDGLFRSTRHATSKFVLSFTVSVLACSYLMHCSIDDYDKLSVDGIIWPLVYVVHNATFCAVFCALPWQTYRHRNRLAAALNTLRQNELSLFDLTGSVTDYRVVKLLAVVIVCNTVFFHTFFHAYYITKTHSPDELFLSVYIVDCTFMYFDVVIELVLGLCDCLLLIAHLQLDRLVWIVKNRDQAAMSIDRVLLTYVTTYNSIATVLGDHLCSYFGPLVLLHCSYTCLEAAICILDTNRHIIKIDGIMSIVANILWPLSDLKKLSAVFLLGEGVNRMVEETALCTRHFDDYRLQNTRAAKQIQKFLLKNLHQKKKFSACGFFDIDNTVIYMVFSSIVTYLVILIQFKQLETDLTQAGDGYNVTSNVSTVQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W131</accession>
    <name>A0A6E8W131_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8W131"/>
    <dbReference type="EnsemblMetazoa" id="ACON009935-RA">
        <property type="protein sequence ID" value="ACON009935-PA"/>
        <property type="gene ID" value="ACON009935"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011871"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON009935"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040987"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026299822" evidence="1">
        <location>
            <begin position="23"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009935-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="74" mass="8426" checksum="23A317B231A2B7FB" modified="2020-08-12" version="1">MGKVVVLVLSIIIGLATQQAMAKEKHIKYTEKDLLRKSIVYDKNTPDVFYCPVNKPTGMDKMIVKRFAKDNVEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W138</accession>
    <name>A0A6E8W138_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phospholipase</fullName>
            <ecNumber evidence="9">3.1.4.4</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1,2-diacyl-sn-glycero-3-phosphate + choline + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14445"/>
            <dbReference type="ChEBI" id="CHEBI:15354"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="EC" id="3.1.4.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endomembrane system</location>
            <topology evidence="8">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the phospholipase D family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.4" evidence="9"/>
    <dbReference type="EnsemblMetazoa" id="ACON010687-RA">
        <property type="protein sequence ID" value="ACON010687-PA"/>
        <property type="gene ID" value="ACON010687"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011989"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON010687"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032898"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004630">
        <property type="term" value="F:phospholipase D activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006654">
        <property type="term" value="P:phosphatidic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009395">
        <property type="term" value="P:phospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060627">
        <property type="term" value="P:regulation of vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01254">
        <property type="entry name" value="PH_PLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09138">
        <property type="entry name" value="PLDc_vPLD1_2_yPLD_like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09141">
        <property type="entry name" value="PLDc_vPLD1_2_yPLD_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06895">
        <property type="entry name" value="PX_PLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000351">
        <property type="entry name" value="Phospholipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1520.10:FF:000055">
        <property type="entry name" value="Phospholipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.870.10:FF:000011">
        <property type="entry name" value="Phospholipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.870.10:FF:000036">
        <property type="entry name" value="Phospholipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.870.10">
        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025202">
        <property type="entry name" value="PLD-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001736">
        <property type="entry name" value="PLipase_D/transphosphatidylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016555">
        <property type="entry name" value="PLipase_D_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015679">
        <property type="entry name" value="PLipase_D_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18896:SF76">
        <property type="entry name" value="PHOSPHOLIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18896">
        <property type="entry name" value="PHOSPHOLIPASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00614">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13091">
        <property type="entry name" value="PLDc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009376">
        <property type="entry name" value="Phospholipase_D_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00155">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56024">
        <property type="entry name" value="Phospholipase D/nuclease"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50035">
        <property type="entry name" value="PLD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 9" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PX" evidence="12">
        <location>
            <begin position="159"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="PLD phosphodiesterase" evidence="11">
        <location>
            <begin position="577"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="domain" description="PLD phosphodiesterase" evidence="11">
        <location>
            <begin position="1095"/>
            <end position="1122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="667"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="724"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="769"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR009376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010687-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1282" mass="143755" checksum="6250D1C64696107A" modified="2023-06-28" version="2">MADEFEHLVSDQMSLSEIGTSNPALNCDDTGTASSNLTAQLDQQGCHGTDSRGAYYNGTTGGSGDGGAGGGAGAGSAGHHDASLACYSIRSEPEEYDENLDAPDSVTYLSIGGNNPVLVIRESDADSVSPASEIPYSYVYNTPVKFDSMRRHIFIPGLEIIVEIIDYERSLTSHVLNPNLYTVKLTHGPFSWTIQKRYNHFRNLHQQLTTYRASLHIPFPTKSHKERRDSFRNMHTVHQATASPAMQQKLGKPLKKIKKSALPRFPLKPDSLVSFDAIPQRIKQLEEYLYNLLNISLYRNFHGTINFLEVSHISFISALGEKGKEGPIKKRTGSTRPGQSGCNFCGCLAGGCCVRCSYFCTDVLWSKWRNRWFFVKETCFGYYRPKDGVLRCVVLFDQGFDISSGMYSTGMRNGLQIATNSRYLVIKYPTRRIAKEWMTHMKRVANESARDFTLPNPHQSFAPSRPGVQAGWFVDGAGYMSAVADALEGATEEIFITDWMLSPEIYMKRPAIDGDYWRLDKILKRKAEQGIKIFVLLFKELDFALGINSYYSKAKLVEQHENIKVMRHPDHARAGILFWAHHEKLVIIDQTYAFVGGIDLCYGRWDDYQHRLTDLGSISSSTNSSANNTTTRKPSTVVELDENGSVANLLKSSKNIAIATAVDRQAPTSVAVKRADTTQPAAKEPVATNGKQKNPAEKNPEAPGAMGDPPDRVLQAAALALTDQLPLEERDELPENIKQNTPEMERRNIMGMIKDMGRDLKNRITLSEHLDHPQGTDALASGGATGGGSGVKSNVGSPIYLSEEERRKKQLYGGEKGAPLGDLASPQPFKNAAIFELDGQAKLWIGKDYINFIVKDFTNLDSPYADLVDRTTTVRMPWHDVATVVLGQAARDVARHFIERWNAVKLEKCRENANFPYLLPKSYNDIRIDSKFLNVPLHHVTCQVLRSASSWNCGFIEPDYVEQSIHEAYVQTISKAQHYIYIENQFFISMELGNSVVKNQISEYLFKRIVRAHREKKVFRVYVVMPLLPGFEGDVGGSSGISLRAITHWNYASISRGKSSLLERLRAAGIQKPCDYISFHSLRTNSTLNGMPVTELIYVHSKLLIADDKVVICGSANINDRSLLGKRDSEVCVMITDESFEEGRMNGESYPCGVYAGKLRKFLFREHLGLLEPDPKRAPVDVTDPVIHTFWNDVWRRTSRRNTLIYDEVFRCIPSDNVQSFAMMKRFLEDKSLLQSNPEGIQQAVERIEGYLVDLPLKFLCNEILTPPNTSKEGIMPTYMWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W148</accession>
    <name>A0A6E8W148_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cytokine-like nuclear factor N-PAC</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Glyoxylate reductase 1 homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Nuclear protein NP60 homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Putative oxidoreductase GLYR1 homolog</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HIBADH-related family. NP60 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8W148"/>
    <dbReference type="EnsemblMetazoa" id="ACON009949-RB">
        <property type="protein sequence ID" value="ACON009949-PB"/>
        <property type="gene ID" value="ACON009949"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000948"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON009949"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033653"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140673">
        <property type="term" value="P:transcription elongation-coupled chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05836">
        <property type="entry name" value="PWWP_GLYR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000058">
        <property type="entry name" value="Putative oxidoreductase GLYR1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006115">
        <property type="entry name" value="6PGDH_NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035501">
        <property type="entry name" value="GLYR1_PWWP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029154">
        <property type="entry name" value="HIBADH-like_NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051265">
        <property type="entry name" value="HIBADH-related_NP60_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000313">
        <property type="entry name" value="PWWP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43580:SF2">
        <property type="entry name" value="CYTOKINE-LIKE NUCLEAR FACTOR N-PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43580">
        <property type="entry name" value="OXIDOREDUCTASE GLYR1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14833">
        <property type="entry name" value="NAD_binding_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03446">
        <property type="entry name" value="NAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00855">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00293">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50812">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PWWP" evidence="9">
        <location>
            <begin position="9"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="127"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00030287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00078412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00082969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009949-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="546" mass="59321" checksum="45CD476E1833EA26" modified="2020-08-12" version="1">MSDSTGYAVNDLVWAKMKGFSPWPGRISVPPAELRKIAVKKNNPVKCIFFFGSNNYAWIEETQIKPYLQFKDTHLNSSKSAQFKEALKQIEEFRVNPEKFQPLFVGENEAANRPDPDEEFNRLREGVASGTEESGAEDGASVNNTTTPAVLESVDEATSTPMVKKSAKKKTSVTKPRSVGNKAKAMLDDGVSGSPSPKRKKKLLNDSGELSSLDASPVRRNAPVTHLLNRPVTVTRPETPEIDMSSVSNAVQSRNIKASQLKFGFLGLGVMGCGIVKNLIKSGHSVVVWNRSAHKCRKFQEVGAEVADTPSDVVEMTDVTYSCVSDPQVAKDMVFGNCGVMSANLAGKGYVEMTGVDPETSNDINEAIISKGGRYLEAQIQGSKNQAEEGTLIILASGDRLLFEECQSCFEAISRNSFYLGDVGNATKMNLILQMISGITLAGVAEAMALADRAGLQQKDVLEVLELTNMSSEMMLQKGNAIIKGEFPTHQALKHMQKDLKLALSLADGLEQSLPITAASNEVYKHAKRLGYGSHDASAVYVRARF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W164</accession>
    <name>A0A6E8W164_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8W164"/>
    <dbReference type="EnsemblMetazoa" id="ACON010292-RA">
        <property type="protein sequence ID" value="ACON010292-PA"/>
        <property type="gene ID" value="ACON010292"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004914"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON010292"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033080"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20810">
        <property type="entry name" value="C1_VAV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21201">
        <property type="entry name" value="CH_VAV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01223">
        <property type="entry name" value="PH_Vav"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09940">
        <property type="entry name" value="SH2_Vav_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037832">
        <property type="entry name" value="PH_Vav"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055251">
        <property type="entry name" value="SOS1_NGEF_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035031">
        <property type="entry name" value="Vav_SH2_invertebrate"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45818">
        <property type="entry name" value="PROTEIN VAV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45818:SF3">
        <property type="entry name" value="PROTEIN VAV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22697">
        <property type="entry name" value="SOS1_NGEF_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 5" id="KW-0727">SH2 domain</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="9">
        <location>
            <begin position="13"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="DH" evidence="8">
        <location>
            <begin position="184"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="7">
        <location>
            <begin position="397"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="10">
        <location>
            <begin position="517"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="6">
        <location>
            <begin position="586"/>
            <end position="690"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010292-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="725" mass="84411" checksum="ED3E5509A851598C" modified="2020-08-12" version="1">MATVTATSNTMVAELWRECAAWLTRCNIIPNDHRANHLDSDIKVLATILRDGVLLCNLANFFDPSSFDRKDFNRKPQMAHFLCIQNIKLFLEACKTNFGLKEADLFEPTMLYDLTNFHRVLLTLSKLSTCRKVQTATNIPTSNPVEYCSHDEHDMKVEEVYQDLCSIQNASRNQLASSSNNLEQRDFVIKELLDTEKNYLEALNALKYIFMQPLEKLLSKEEIRAIFPCIRELVEIHNKFLDRLHEAVCPGSKLKLSTTFLEFREPFLIYGEYCSSMTSAVETLREACKKSNTVEQTVLQSQKEHSDGRLQLRDILSVPMQRILKYHLLLDKLVQETVPTHDDFRGLERAKEAMVDVAQYSNEVKRDSEHLIVIQKVKESISDLNLPNGNNLEQYGRLLLDGDLNIKAHEDQKMKHRYAFVFEKVMILVKNSNTKIGEAQYSFREAHNLLDYRIEILNSRRTLGRDGRLKYSLLLARKTQATAFTLYMKTEEEREKWKRAFETAMETLEPVGCKNTDHKFVIHTFDTPVICRHCSKFLKGKIHQGYRCKVCEIVVHRGCISSTGRCKQLQQTPPPVCDRLLSEFNWFVGTMNRDSATQRLENKKIGTYLLRVRPQGASHESETIYALSLKTDKKIIKHMKIYQKKEHQNVFYYLSTRRYFKTIIELVSFYERNDLGENFAGLNQLLQWPFKEEIVVAIYDFAPNELNQLPMRGMSGDCNWKRRRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W2C9</accession>
    <name>A0A6E8W2C9_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Endophilin-A</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
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            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8W2C9"/>
    <dbReference type="EnsemblMetazoa" id="ACON010376-RC">
        <property type="protein sequence ID" value="ACON010376-PC"/>
        <property type="gene ID" value="ACON010376"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001275"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON010376"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038642"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07594">
        <property type="entry name" value="BAR_Endophilin_B"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
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    </dbReference>
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        <property type="entry name" value="SH3 Domains"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
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        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SH3_9"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
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    </dbReference>
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        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="BAR" evidence="2">
        <location>
            <begin position="29"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010376-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="351" mass="39337" checksum="175DD58236EFD6C6" modified="2020-08-12" version="1">MNIKMPDFDVKKFVKGAGSTLSRVVQLTEEKLGTSEKTEMDARFEHLSERSDSAKLWTEKIMRDTEAALIPNPANRVEDFIFEKIEKQKPKRLSNLEYLGLDMIEGGGEFGQDGPYGSALIKVGQAEQKLGSCERDFIGSAGMCFIQPLKKFLEGEMKTITKEKGVLESKRLDLDACKNRVRKARSMIGQQAAERDLRVAQSEFDRQAEITKLLLEGISTTQATHLRYLHSLVEAQVRYYGQCNKIMSDLQRELVSLGGPQPYVPVSGYEDEDALSERVNNIELGANSGYRRARVLCSYDAKDGTELNLSSNEAVIFICECNPPHSDYMNGKQGLLKGLVPKAFLEMLDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W2D0</accession>
    <name>A0A6E8W2D0_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">HIG1 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8W2D0"/>
    <dbReference type="EnsemblMetazoa" id="ACON010226-RA">
        <property type="protein sequence ID" value="ACON010226-PA"/>
        <property type="gene ID" value="ACON010226"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACON010226"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010226-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="44" mass="4789" checksum="04B35B286AF7978C" modified="2020-08-12" version="1">VNEVASKSTDRYTTRSAALLAALGIGLSSFSMKQMLSKQTKRKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W2D9</accession>
    <name>A0A6E8W2D9_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Galectin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8W2D9"/>
    <dbReference type="EnsemblMetazoa" id="ACON011287-RA">
        <property type="protein sequence ID" value="ACON011287-PA"/>
        <property type="gene ID" value="ACON011287"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000032"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON011287"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030049"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016936">
        <property type="term" value="F:galactoside binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00070">
        <property type="entry name" value="GLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000256">
        <property type="entry name" value="Galectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044156">
        <property type="entry name" value="Galectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001079">
        <property type="entry name" value="Galectin_CRD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11346:SF176">
        <property type="entry name" value="32 KDA BETA-GALACTOSIDE-BINDING LECTIN LEC-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11346">
        <property type="entry name" value="GALECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00337">
        <property type="entry name" value="Gal-bind_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00908">
        <property type="entry name" value="Gal-bind_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00276">
        <property type="entry name" value="GLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51304">
        <property type="entry name" value="GALECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0430">Lectin</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Galectin" evidence="4">
        <location>
            <begin position="13"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="187"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="187"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="199"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="213"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU102079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON011287-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="268" mass="28535" checksum="2C1697C901EFC63D" modified="2020-08-12" version="1">MATIPQYSPTLPFLGLIPGGLSPGRMLRIKGIITNHGERCQIYIQSGAAVNPRDDVTLHISIRPHEHAIVRNSIQNQVVGPEERYGGCPIRYGESFDLLILAEATQYKIAINGAHFCTFGHRLPLHRAQYISIAAGGTIYSILSEADVPGSVPVNPYPPIVHLPPAAPYAPPAMGFVPNAPPSNLPPPPPYTPLPSHPIGGGSGHYPGVGGYPLPPPPPPPPSTMPGYPHYPTQPPGAPLPAAVPFSVLFKEELQAKVEQIKGKPADY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W2V3</accession>
    <name>A0A6E8W2V3_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Kinesin-like protein unc-104</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Required for presynaptic maturation, has a role in axonal transport of dense-core vesicles carrying synaptic vesicle precursors, components required for the morphological transformation of axonal growth cones to mature boutons.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON010519-RI">
        <property type="protein sequence ID" value="ACON010519-PI"/>
        <property type="gene ID" value="ACON010519"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001094"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON010519"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037311"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904115">
        <property type="term" value="C:axon cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048490">
        <property type="term" value="P:anterograde synaptic vesicle transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021700">
        <property type="term" value="P:developmental maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051222">
        <property type="term" value="P:positive regulation of protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040012">
        <property type="term" value="P:regulation of locomotion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051960">
        <property type="term" value="P:regulation of nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010975">
        <property type="term" value="P:regulation of neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008582">
        <property type="term" value="P:regulation of synaptic assembly at neuromuscular junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd22705">
        <property type="entry name" value="FHA_KIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01365">
        <property type="entry name" value="KISc_KIF1A_KIF1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01233">
        <property type="entry name" value="PH_KIFIA_KIFIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.20:FF:000001">
        <property type="entry name" value="Kinesin family member 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000004">
        <property type="entry name" value="Kinesin-like protein isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000204">
        <property type="entry name" value="kinesin-like protein unc-104 isoform X6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2520">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022164">
        <property type="entry name" value="Kinesin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022140">
        <property type="entry name" value="Kinesin-like_KIF1-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032405">
        <property type="entry name" value="Kinesin_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049780">
        <property type="entry name" value="PH_KIFIA_KIFIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47117:SF10">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIF1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47117">
        <property type="entry name" value="STAR-RELATED LIPID TRANSFER PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12473">
        <property type="entry name" value="DUF3694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12423">
        <property type="entry name" value="KIF1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16183">
        <property type="entry name" value="Kinesin_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="9 12" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="16">
        <location>
            <begin position="3"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="15">
        <location>
            <begin position="1556"/>
            <end position="1654"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="398"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="966"/>
            <end position="998"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="359"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="631"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="398"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="412"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="982"/>
            <end position="997"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="97"/>
            <end position="104"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00056070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010519-PI"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1658" mass="187150" checksum="D3A525B91C5BBE69" modified="2020-08-12" version="1">MSSVKVAVRVRPFNSREIARESKCIIEMAGNTTCITNPKVPPGSSESVKRFNYDYSYWSHDPRDLEFSTQAMVYSDIGEEMLQHSFDGYNVCIFAYGQTGAGKSYTMMGKQEDGQEGVIPMICKDLFRRIQETESDDLKYSVEVSYMEIYCERVRDLLNPKNKGNLKVREHPLLGPYVEDLSKLAVTSYQDIHDLIDEGNKARTVAATNMNETSSRSHAVFTIFFTQKRQDRMTSLETEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEIASKNKKSKKADFIPYRDSVLTWLLRENLGGNSKTAMIAAISPADINYDETLSTLRYADRAKQIVCKAVVNEDANAKLIRELKEEIQKLRELLKAEGIEVQEGPDGKVVCEKRDANSEDGEKKIHSPNRNRKRTGSSTEMAVDQLQASEKLIAELNETWEEKLKRTEQIRVQREAVFAEMGVAVKEDGITVGVFSPKKSPHLVNLNEDPTLSECLLYYIKDGLTRLGTSEANVPQDIQLSGSHILKEHCVFENKDGVVTLVPHKDALVYVNGRKVIEPEVLQTGSRVILGRNHVFRFTHPEQAREKREKNKEVDVCETPGNSGEIADWNFAQCELLEKQGIDLKAEMEKRLLALEEQYKREKRAADQEFEEQRKTYEARIDALQKQVEEQSMTMSMYSSYSPEDFHQEEDIFVNPLFESCWTAREAGLAAWAFRKWRYHQFTSLRDDLWGNAIFLKEANAISVELKKKVQFQFTLLTDTLYSPLPPELTPAPAVGALTNGGQEDEFGQNPIPRTTVAVEVTDTKNGATHYWSLEKLRQRLELMREMYHNEAELSPTSPDYNVESLTGGDPFYDRFPWFRMVGRSFVYLSNLLYPVPLVHKVAIVNERGDVRGYLRVAVQPVMDEENADFSNGVKQSARILFDEEQNGQHKVPKIRTIPDKDEKYIEGGNEMGTKLEELEQEDADSGRGDSSVASELHESNDQEPGEHLQPGKEFTFRVTVLQATGIAAEYADIFCQFNFLHRHEEAFSTEPVKNSGSGAPLGFYHVQNITVPVTKSFIEYLKTQPIVFKVFGHYQHHPLHKDAKQDCQITRPPPRRMLPPSIPISQPVRSPKFGPLPCPPSSTVLAKHDVLVWFEICELAPNGEYVPAVVDHSDDLPCRGLFLLHQGIQRRIRITIVHEPTPEVKWKDIRELVVGRIRNQPEPADDEDSDSCVLSLGLFPGEVLEVPGDDRSFFRFEAAWDSSLHNSALLNRVTQTGEQIYITLSAYLELDNCARPAIITKDLSMIIYGRDARTGPRSLKHLFSGQYRNPEANRLSGVYELSLRRASEAGSPGVQRRQRRVLDTSSTYVRGEENLHGWRPRGDSLIFDHQWELEKLTRLEEVGRVRHLLLLRERLGMDTTPNPTTKTEKDVCNLAQRASASPVHMVIPPSPQTPVKDQQAPAMPERELSVRETELVWKCVKLIQGRIGCKELGAEGNGSGTQASDASPGDEGCADMNASYISSNSIELCSPERVDMPNGWEAPAPAPQTQDLSLRLYVPELEEIRVSPVVARKGYLNVLEHGGSGWKKRWVTVRRPYVFIFRSDKDPVERAVLNLATAQVECSEDQAAMVKVPNTFSVVTKHRGYLLQTLGDKEVHDWLYAINPLLAGQIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W2Y0</accession>
    <name>A0A6E8W2Y0_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">Ngousso</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8W2Y0"/>
    <dbReference type="EnsemblMetazoa" id="ACON010961-RA">
        <property type="protein sequence ID" value="ACON010961-PA"/>
        <property type="gene ID" value="ACON010961"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_013606"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON010961"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031056"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11056">
        <property type="entry name" value="CYP6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 13 14" id="KW-0349">Heme</keyword>
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    <keyword evidence="6 13 14" id="KW-0479">Metal-binding</keyword>
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    <feature type="binding site" description="axial binding residue" evidence="13">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
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            <dbReference type="ARBA" id="ARBA00001971"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004174"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00004406"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00010617"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022824"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010961-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="361" mass="41167" checksum="E78CA8A253096EDC" modified="2020-08-12" version="1">VGADMMASLDALPVLRPNVRETEFKELCARFTTDVIASTFFGMQANCLKDEQSEFRYYGRKIFDYGPGRGLTMASFFFLPELVPYLRLKLFPRDTEAFLKAIIEQEIARREQTGESRGDFIDSMIALKNNNATLGVNEKIPLKGDILVAQAATFYMASFETTSSVLSFALYELTRNPHVQERLREEIRDIIVKYGREIPYEAYANEMPYLGMVISETNRLYPVLAFIERQCTLPEGSTGYKLEPLHDYVIPNGMPIMIPIYAIHRDPKYFPNPTVFDPERFAKENLDQIQPCTYMPFGVGPRTCLGSHFGTLQIKIALLKLLSQYRIERSPSTPEVLTYRKNAFTLNSNEGLYAALVEDAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W4H2</accession>
    <name>A0A6E8W4H2_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">LIM zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Nematocera</taxon>
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            <taxon>Anopheles</taxon>
        </lineage>
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        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8W4H2"/>
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        <property type="gene ID" value="ACON012305"/>
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    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041612"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:Z disc"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042805">
        <property type="term" value="F:actinin binding"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:structural constituent of muscle"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:muscle organ development"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="MUSCLE LIM PROTEIN MLP84B"/>
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        <property type="entry name" value="RHO-GTPASE-ACTIVATING PROTEIN LRG1"/>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="6 8" id="KW-0440">LIM domain</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0517">Myogenesis</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="9">
        <location>
            <begin position="9"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="9">
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            <end position="171"/>
        </location>
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            <end position="273"/>
        </location>
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        </location>
    </feature>
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            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022541"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022723"/>
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        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblMetazoa" id="ACON012305-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="397" mass="42818" checksum="5B5E1251A70A69E4" modified="2020-08-12" version="1">MPFKPADNPKCPKCGKSVYAAEERVAGGNKWHKQCFKCGMCGKMLDSTNCAEHENELYCKNCHGRKYGPKGYGFGGGAGCLSMDTGAQFQGEGTNGHFEPKPMSKAPEGEGCPRCGGYVYMAEQMLARGRAYHKGCFKCGECKKGLDSVNCCEGPDRNIYCKVCYGKKYGPKGYGYGQGGGALQSDTTNIECEFAPKPSAVDTSAIKASPGQGCPRCGGVVFAAEQVLSKGREWHRKCYKCRDCSKTLDSIIACDGPDRDVYCKTCYGKKWGPHGYGFACGSGFLQTDCMSEEQLASQKPMVTIDTTVIKAPEGQGCPRCGGMVFAAEQQLAKGTMWHKKCFNCNECHRPLDSMIACDGPDREIHCRACYGKLFGPKGFGFGHTPTLVSTDAQAAPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W4Z3</accession>
    <name>A0A6E8W4Z3_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8W4Z3"/>
    <dbReference type="EnsemblMetazoa" id="ACON011847-RA">
        <property type="protein sequence ID" value="ACON011847-PA"/>
        <property type="gene ID" value="ACON011847"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACON011847"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON011847-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="19" mass="2257" checksum="FA114AB703C10AF1" modified="2020-08-12" version="1">MLDSPRCQQEKPSFRLYAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W6M0</accession>
    <name>A0A6E8W6M0_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Nuclear factor of activated T-cells 5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">T-cell transcription factor NFAT5</fullName>
        </alternativeName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="23">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Transcription factor involved, among others, in the transcriptional regulation of osmoprotective and inflammatory genes. Binds the DNA consensus sequence 5'-[ACT][AG]TGGAAA[CAT]A[TA][ATC][CA][ATG][GT][GAC][CG][CT]-3'. Mediates the transcriptional response to hypertonicity. Positively regulates the transcription of LCN2 and S100A4 genes; optimal transactivation of these genes requires the presence of DDX5/DDX17. Also involved in the DNA damage response by preventing formation of R-loops; R-loops are composed of a DNA:RNA hybrid and the associated non-template single-stranded DNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="18">Homodimer when bound to DNA, completely encircles its DNA target. Interacts with CIDEC; this interaction is direct and retains NFAT5 in the cytoplasm. Does not bind with Fos and Jun transcription factors. Interacts with DDX5 and DDX17; this interaction leads to DDX5/DDX17 recruitment to LNC2 and S100A4 promoters and NFAT5-mediated DDX5/DDX17-enhanced transactivation.</text>
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        <subcellularLocation>
            <location evidence="2">Chromosome</location>
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            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
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        </subcellularLocation>
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    <sequence length="925" mass="97483" checksum="340D7AD7B4256439" modified="2020-08-12" version="1">MVATSVSPLSPQTLSPSTGSSSPSTISPGVSLAASPLASCTGRQNAPAGGGARTANSNRGATASSVPFRMAPGTDGSFSVAGVPPALISSRDGKIQLQIVTQPEQQHRARYQTEGSRGAVKDRSGNGFPVVRLVGYNKPAALLVYIGSDVGRPTPHIFYQACKVSGKNSTPCVERILEGTKYIEVQLKPENNMTVTCDCVGILKERNVDVEYRFPDQTASRTKKKSTRCRMVFRTTITGEDGATETLQVSSHQIICTQPPGVPEILKKSLISCPVEGGLELFIIGKNFLKDTRVVFHRPKAVPAHSAFTRTAAAGAAWEHAVAPDKEHLNQVHLICKVPPYERQDITEPVTIKLYILSSGKKSETHDFIYTPKGEHTALSAATTVATIPGRRNRLGTAVGQQQQQQQQPPPPQHQQQQPPPTSHNYFSELAGLGNNGAGVQNGGNVTAVPNVDGAALANAINAIAATAAGMGELPGPNGAAGGRFICNGNRTEAAVSPFDEPANVHASRPMFLRTSTPLEIEAKGMMPPPINVLPTGSIIPPQCHPGVLSLPSPNQPIQPAVDVFKPELVEPECSRSHMADEESLDQFAVKASLARFGASVENSLDGILFAPVPAEPSILYRRRSVRQPSMEMEDSCSNMSLLANDSRMIDLPSAGGAAAAAAAGGGGSSGGGSLSIGSHFDTVMNIATLTSFCGSLPVAPMETNYMGGEPLGTAKPPPLPVDCDIKSIIKTEPQTPHASTTPVNMQLDQLLAARRVQRRDCHADRGNVCCSSNSSSSNNNNNNNNNNSSSSNNSSSRNSNNSSNNNNSNNNSNNSSSNNSSSNNSSSNSSNNNSSSNNNNNNNSSNSSRNNTSNNSNSSSSSNNNNNSINKLNNSNNNNCSSNNNNCINKLNNNSNNNNNNNSCSNSINKLNNNSSNNCSSKHC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W9F9</accession>
    <name>A0A6E8W9F9_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="4">FSA_C domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="EnsemblMetazoa" id="ACON029237-RA">
        <property type="protein sequence ID" value="ACON029237-PA"/>
        <property type="gene ID" value="ACON029237"/>
    </dbReference>
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    <dbReference type="VEuPathDB" id="VectorBase:ACON029237"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="project" value="GOC"/>
    </dbReference>
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        <property type="term" value="P:synaptic vesicle endocytosis"/>
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    </dbReference>
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        <property type="entry name" value="BLTP1"/>
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        <property type="entry name" value="BLTP1_N"/>
    </dbReference>
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        <property type="entry name" value="BRIDGE-LIKE LIPID TRANSFER PROTEIN FAMILY MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TRANSMEMBRANE PROTEIN KIAA1109"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="BLTP1_C"/>
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        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
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    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="Bridge-like lipid transfer protein family member 1 C-terminal" evidence="3">
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</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WAA3</accession>
    <name>A0A6E8WAA3_ANOCL</name>
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        <recommendedName>
            <fullName evidence="2 14">Protein kinase C</fullName>
            <ecNumber evidence="2 14">2.7.11.13</ecNumber>
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        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
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            <dbReference type="DOI" id="10.3390/genes10010062"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain>Mali-NIH</strain>
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    <reference evidence="23" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="23">Ngousso</strain>
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        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
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        <reaction evidence="12 14">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
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    <comment type="similarity">
        <text evidence="1 14">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.</text>
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        <property type="component" value="Unplaced"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ZF_DAG_PE_2"/>
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    </dbReference>
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    <keyword evidence="11 14 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 14 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PB1" evidence="22">
        <location>
            <begin position="21"/>
            <end position="104"/>
        </location>
    </feature>
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            <begin position="264"/>
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        <location>
            <begin position="548"/>
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        <location>
            <begin position="580"/>
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            <begin position="270"/>
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        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
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            <position position="293"/>
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    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR000554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000554-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR000554-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON029538-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="589" mass="67261" checksum="C52CAB3B4912BA8E" modified="2020-08-12" version="1">MPIQIANESNNSGGGGGSGHEIKVKIAYNGEVMITYIDESITYDGLCREMREICRFSPDQVFTMKWVDEENDPCTIQSDTELDEAIRLYEVNRDSELVIHVFPNVPSAPGLSCVGEDRSIYRRGARRWRKLYRINGHIFQAKRFNRVSSSSSCKAFCAFCHDRIWGLGRQGFKCIQCKLLVHKKCHKLGNKPCSNEHVEPVFKERDHHDPNDESSTGDVIQAPLDYPSELHSGDGCDQVPVEVNDNVEETLEPVTQRQYSLNDFELIRVIGRGSYAKVLMVELKKTRRIYAMKVIKKALVTDDEDIDWVQTEKHVFETASNHPFLVGLHSCFQTPSRLFFVIEFVRGGDLMFHMQRQRRLPEEHARFYAAEISLALNFLHEKGIIYRDLKLDNVLLDHEGHIKLTDYGMCKEGIRPGDTTSTFCGTPNYIAPEILRGEDYGFSVDWWALGVLLYEMLAGRSPFDIAGASENPDQNTEDYLFQVILEKTIRIPRSLSVKAASVLKGFLNKNPADRLGCNRDSAFLDIMNHSFFKSIDWEMLEQKQITPPFRPRLDSDRDLANFPPEFTDEPVHLTPDDESINPSSKDSSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WAA8</accession>
    <name>A0A6E8WAA8_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Angiomotin C-terminal domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the angiomotin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8WAA8"/>
    <dbReference type="EnsemblMetazoa" id="ACON029540-RA">
        <property type="protein sequence ID" value="ACON029540-PA"/>
        <property type="gene ID" value="ACON029540"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004393"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON029540"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032577"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030334">
        <property type="term" value="P:regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009114">
        <property type="entry name" value="Angiomotin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051747">
        <property type="entry name" value="Angiomotin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024646">
        <property type="entry name" value="Angiomotin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14826">
        <property type="entry name" value="ANGIOMOTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14826:SF14">
        <property type="entry name" value="ANGIOMOTIN_C DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12240">
        <property type="entry name" value="Angiomotin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01807">
        <property type="entry name" value="ANGIOMOTIN"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0965">Cell junction</keyword>
    <keyword evidence="5 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Angiomotin C-terminal" evidence="8">
        <location>
            <begin position="540"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="245"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="304"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="791"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="892"/>
            <end position="1054"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="440"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="496"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="838"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="8"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="37"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="67"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="78"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="98"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="115"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="134"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="155"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="200"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="308"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="791"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="807"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="901"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="929"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="940"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1003"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1039"/>
            <end position="1048"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON029540-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1054" mass="111227" checksum="9E58D969A0335F64" modified="2020-08-12" version="1">MSHGGATRYSQYASNGAGPQQSNGTGSGKASSNNAAQQQQQQQQPQVPSLPFMRPPMLVTSSGASMLPPPPCPQTPPTGGGSGGGGGGGLPKYPGSPNGSLSGSDTDVSTSNENLSREERYVLSHARVEPQGEENLQGNVTPVNEIQGRFIGDKFSSNTSSDSTRYATPTGGSSSRSMSETRGGGGGMYANIGSAPDGPSNRSSMAGSNRNSLKEPNSNRSSMDVSQSSYNTLIIHDDIYSSVASSGREYAGSPPPYMKKERPRSYGEAAPAAPPMQDISEINEEYINKSHVLKHLAKEVKLSRATTARNSRQQSSSGGSAVSNGGGSDVGEQKMSSHGTGGSKAARRGSEAATGGGSGNSAAKLKSKSQPDLTRMYDIDPEEVEALVKENQVLKQQLNNCYLKVAKSQKLEQEIANIYRVHEELVQSCDRRERLERAARTRLQQDCRRLQELNRAFRDQVEVYQSQLHASSDHQLGRTQQDVLISQLVNQNKSLIDANRRQCIELQAQNATLEEQRIHINVLDTALKRLEEECRQKQVYVERCAQLQHALQSLQNASERREQAERKLRIDYDNDMANRSGSSTNSETDNLKWQLREKDAQILRLEAECAQLEQRNLEESNVRNVAKLAMERNSQETERILAEAKQEKIRYLDEAHAANRKVTELQTRLKLVENRLAEKDAMIRAYQGQKIYGGSTNSYGSYNLSSDSFALNPLGAYNSQASYVDVSSGAYDVATGAGASLLDPSSVVVGPYGPAATAAVVSSYASTYGPTTVSNASSFAQSSLNLPTGAGVGGGSGAGGGPGGPVGAYSSPGSSSTTSNYSSTANFGAGSEHTIYDSKSYDAQRKSIDDQLKQLDEQLLSKVSELSLVEKQRQLNQQARALAAKLKAVSASGGRAGSSGLGTASETSSAGSSVDANGNGSTLLHAAPTNLSKPSGTRNTATSTTTIASSTTSSSSCVGGAGGKQLRSKAPAPSIAASTKKHAHHSMAKTTSTPAIATKKSHGAAATASVGTNTTGSATVAGSSSNHGKKLAASCKNVSTSATDSSSSEPETFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WAV5</accession>
    <name>A0A6E8WAV5_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="16">PDZ domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON029508-RA">
        <property type="protein sequence ID" value="ACON029508-PA"/>
        <property type="gene ID" value="ACON029508"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002482"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009205"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012236"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON029508"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030142"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030881"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035730"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045197">
        <property type="term" value="P:establishment or maintenance of epithelial cell apical/basal polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:neurotransmitter receptor transport postsynaptic membrane to endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:receptor clustering"/>
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        <property type="entry name" value="protein lap4 isoform X1"/>
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        <property type="entry name" value="PROTEIN LAP4"/>
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        <property type="match status" value="9"/>
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    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="10" id="KW-0221">Differentiation</keyword>
    <keyword evidence="8" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
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        <location>
            <begin position="669"/>
            <end position="756"/>
        </location>
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    <feature type="domain" description="PDZ" evidence="15">
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
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            <dbReference type="PROSITE" id="PS50106"/>
        </source>
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            <dbReference type="EnsemblMetazoa" id="ACON029508-PA"/>
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    </evidence>
    <evidence type="ECO:0000313" key="17">
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            <dbReference type="Proteomes" id="UP001105220"/>
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    <sequence length="2692" mass="292902" checksum="4718860C55FA8601" modified="2020-08-12" version="1">MFKYIPIFKGCNRQIEYVDNRHSSLPNVPEEIFRYSNSLEELLLDANHIRDLPKGFFRLYRLRKLGLSDNDIIKIPSDIQNFVNLVELDVSRNEIGDIPEDIKHLRSLQIADFSSNPISRLPAGFTQLRNLTVLGLNDMSLISLPQDFGCLSKLVSLELRENLLKNLPESISQLTKLERLDLGDNEIDELPSHVGYLPSLQELWLDHNQLLRLPPEIGLLKKLVCLDVSENRLEELPEEIGGLECLTDLHLSQNLLETLPNGISKLTNLSILKLDQNRLHTLNDSIGCCVHMQELILTENFLSELPATVGNMLVLNNLNVDRNSLVAVPSELGNCRQLGVLSLRENKLTRLPAELGNCGELHVLDVSGNLLQHLPYSLVNLQLKAVWLSENQSQPVPTFQPDVDETTNEQVLTCFLLPQQRYVPSNPSQREDTDSENWEEREASRTHSVKFTEDFGADRDTPFVRQNTPHPKELKMKAHKLFAKERKSDDLSGNLDTLSEESASRPSLLNRSGYGTATMEDIQGHAIPHDEEQGEAAAGSAEGGGGVEGAQDDEDGYEKRVGFEVNEDEDDDPERQQYGEDGDEHGGKKPISKLHRRDTPHHLKNKRVHHTITDKNTNLMFNSLKLKEMTPPKLDEHPAMEPPVPPSATASIAEDTLDTYTALKEERLEIHIERTSAGLGLSIAGGRGSTPFKGDDEGIFISRVTERGPADLAGLKVGDKVLKVNGISVEDADHYDAVEVLKACGSVLILYIAREVTRLIGHPVFDDTGSVAQISVSDQDALKPPPLVGAPLGLPTVDGAGVPPYMGALSPNTVGLPNGGAGGVGGGALDMPNGNEITQKIILHTTLIRDQIGQGLGFSIAGGKGHAPFKDGSEGIYISRLTENGVAHKDGKIMVGDRVLAINGVDITNAHHDYAVQLLTDHQRFVRLVVQREVKGPLEPLHSPRSPVVGPNRPPAAAAGAYLPNRLARPMTSADFQAMIPSHFISGGGRQVTVDKSDHGPSVTVTVQKAVPDMPMLPPAPTELGTVTETITKSTFTETVMTRVTDNRLLEPLISEEVILPKDQGSLGFSIIGGTDHSCTPFGANEPGIFISHIVAGGIAALSGKLRMGDRILKVNGTDVTQATHQEAVMELLRPCDDIKLTVQHDPLPAGFQEVHIVKQDGERLGMHIKGGLNGQRGNPLDNADEGVFISKINANGAAKRDGRLRVGMRILEVNGLSLLGASHQEAVDALRASGSKLHLVVCKGYEKSDLLHLAGSAGGMSTGLSNNGDSARIGSRASETGSELSQSVSSLDRDDSVIMAEAHRKLSVDKIDEETLPPQPAVHDHHPVDQPEPPTSQALISVNPNDQQVAIGPKEKSTPEKVLDIVRAAESLALGSQLVENGPPKSPTEVASEPLQKTTTIVMSKHTLDTQAPQVERRFGSVRSLSHANEPPISSINSSTATVATNPATTDTNYHHPPKGDQKIVRPHRPPPPPPPPLAAASTHTTTIPSPSAGAYLDRPPSTKPPPPPPKVEPGSSSSTTTTTSHRKSVSFDLHEDAHGFDTALHHARAAFAGPSPTPDLKGILRASSPSNRSSSSTPEGVQQVQPTVANLDSDPDTDSDTGTESDTDSDASGDSHELLPAAPDGQLAREQPPASNPIRRAEVVRNQAARKLQEEFGQGDLVEYEHDTATNTIREVSVGPGPAARGRPERVEMLPAKFETLPVKRTAQFVHENTPNNLLVPDEVHRQVLLEENEVRNKLLAQYAHEQQQQQQQQHHHHHQQQHMYTMHHHHHTGELLGRVPSPYHLYDSGSASVSPLPSHYIPGSSSSSVYDHHQHLGKLGGPLSPPPPLPSTAPPSDVGYHHHHLPQIYPPVQILPVHYTKLPTPQHTIYTYASGSVPVSHAPIVSLAASTSSQPVPYSSPYHLPPYAGTSAGPTYLSTAHPSSSIAAGPTHPHHQVLMPSPPLGPKTPKSVSDKKRFFENAMEDQQKPTPKSEKVFSFLSQDEVEKLRQEEERKIASLGKDRVHQRFGDEEDEDDDNRSDGGDRPPGDKSDINDNNMREESATGRTEEDEQGRVAGGLLTAASPLAKTPMSRIPIRTVNAEKRARASNCLPPEYDESKLSPSEIRALRAQKRAAWRQARLKSLENDALQAQIMIQTMAAGLGLHEDSLLNSSSASEGVDGSFSRSALAASNVSPLPAAQSPLPTPPAPSADNNDEHEPSANGDDDDDDHHDGRSSSADEAGGADDRLQRRFPRLAVKSRPGSEIVVRETEKVVEEKVTRRTEEAPGGGLRTVEYIEKVIETEVETMREKIIMFELEDSSSADSERDGGGKVEAKNGMPTDQADGTGRWVDGSEPAVHQPDQPVQLQTIVEVINPMLSGDGQPNDIAIGRYSDVAYGEEDSLVVDGVEDATATDATTPVEYAGRYAYEHGAYESINDKMKHVLRELKQNEKVRQNLSKSLTEEEILALQAQELAEQEEDGLEEEEEEEEDVREQYEEKTGAIGTVFMVRERLINDFYTHQTELAHEQQQQQQRKYHQQKLMDDPASYRDCEVITNPSADRFSPTFDHHSGPAGQLYHQTIHSEKLRAVFGSNDGDEDDDDDDECEEERAEDRRNGNTIGGVVAPAIEPATAAGVEEQEEEEEDEEEDDVIIAPSRAVAATAAVEPSNGTSSNASSATTATTTTTTTTATAVPSSTGNNKRKKRKGKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WB42</accession>
    <name>A0A6E8WB42_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Doublecortin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">Ngousso</strain>
        </source>
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    <dbReference type="EnsemblMetazoa" id="ACON029815-RB">
        <property type="protein sequence ID" value="ACON029815-PB"/>
        <property type="gene ID" value="ACON029815"/>
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    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010695"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON029815"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038503"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
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        <property type="term" value="P:intracellular signal transduction"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Echinoderm microtubule-associated protein-like CG42247"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Echinoderm microtubule-associated protein-like CG42247"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Doublecortin domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
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        <property type="entry name" value="Beta-prop_EML-like_2nd"/>
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        <property type="entry name" value="ECHINODERM MICROTUBULE-ASSOCIATED PROTEIN-LIKE CG42247"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="Pfam" id="PF23409">
        <property type="entry name" value="Beta-prop_EML"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Beta-prop_EML_2"/>
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        <property type="entry name" value="WD40"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89837">
        <property type="entry name" value="Doublecortin (DC)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50952">
        <property type="entry name" value="Soluble quinoprotein glucose dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50309">
        <property type="entry name" value="DC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Doublecortin" evidence="5">
        <location>
            <begin position="79"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="491"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="580"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="159"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="191"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON029815-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="859" mass="95154" checksum="46FE0A3430C69418" modified="2020-08-12" version="1">MFDSEEESAFVILTAPMYHPHHYGGGGIGGIYSQNGVWYASPGNQGWGSGAGPARLGRKPSVTEVDNMPSGSLKPSAGRVIRIINSHDHSVQCRVLLNLRTSQPFEEVLEDLGQVLKMIGAKKMYTSNGQEVRSFSQLRNEFAEVETFYLSNTPSLPVGALGPGVIPPSPGRRSRSRLGGSVPDDLSKTTARQRARSKSRPRVLYAPENELVRASSDYPLLDAMKEEPTRITIRGLRRTFYPPLHHPPIDNAPPDKKLSLFWVHGYRGIDSKRNLWVLPSGELLYYVAAVAVLYDREEDAQRHYTGHTEDIMCMEVHPSRELVGSGQRAGRDRKSQAHVRIWSTESLQTLYVFGMGELDSGVIAVAFSQLNGGSYILAVDAGRESILSVWQWQWGHLLGKVATLQEGIWGATFHPLDDNLLITHGRGHLAFWHRRKDGFFEKTDIIKPPSRTFVTCVQFEPDGDVITADSDGFITIYSVDADGAYFVRMEFEAHNKAISCLVMLSEGTLISGGEKDRKIAAWDSLQNYKRITDIKLPESAGGVRSIYPQRPGRNDGNIYVGTTRNNILEGSLQRRFNQVIFGHGKHLWALAAHPDDEVFATGGHDKYVALWRRQKLIWTTSVGYEIIALAFHPYGAALAAGSSEGHLIVINAENGATMLTIRVCGSPLNAVEFNQVGDMIAIGSQNGSIYLFRVSRDGFSYKKINKIRGSQPLTHLDWSSEGNFLQSVTVDFDLLFWDVKSLSPEKSPIAMKDVKWMTSNCTVGFLVAGLWNNRYYPAPSNTIITTTARTAAQDLVAAGDADGYLRLFRYPCITPRAEFTEAKVYSGTLAAVKFLYGNHSLVTVGGTDASLMIWELTEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WB53</accession>
    <name>A0A6E8WB53_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="5">BTB domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8WB53"/>
    <dbReference type="EnsemblMetazoa" id="ACON029581-RA">
        <property type="protein sequence ID" value="ACON029581-PA"/>
        <property type="gene ID" value="ACON029581"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004439"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON029581"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033539"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18315">
        <property type="entry name" value="BTB_POZ_BAB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051095">
        <property type="entry name" value="Dros_DevTransReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110">
        <property type="entry name" value="BTB DOMAIN TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110:SF108">
        <property type="entry name" value="LD19131P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="4">
        <location>
            <begin position="37"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="133"/>
            <end position="197"/>
        </location>
    </feature>
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        <location>
            <begin position="217"/>
            <end position="236"/>
        </location>
    </feature>
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        <location>
            <begin position="246"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="422"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="134"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="179"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="221"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON029581-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="466" mass="50675" checksum="4C7B7D2DF205C37A" modified="2020-08-12" version="1">MSQAAAGLSQQFCVRWNSHLGSLGAAFPQVRKPSPFVDVTLACEGHQVHCHRLVLAACSTYFENLLGENPCKHPIIILPRDIKLWAIQALVDFMYKGEVNVSQAGLPDLMKCAEVLKIRGLCGSDAALNLNQVHSPSGSHQYQQQQHSSANSSDAGEPVEHAGVTARASEGPYAGRHGGQSQSARMQQSNASSGPKRQLLNSLNLQPIDAGIALPHHHHHQQNEDGVHSDNGESGNEMCIKTEDLIIDEDDSGSRQCGDEDELGMDSTDKTGSDLPSEFALANVTCEYELNTQPQAPQDTSTAGRRVRRSEELLQQAAEYVSRGETFQNVSLKFDIPISTIRFYMARKGILPRRKRGRTAMFPCSVSNRPILAAVDMMAPSPTSSFIPQPTLLDQQQQQQYRQQLSENMSIAGTQSAIAQTANVSQTAGAPERVPPNPGGSRSPSPSGPPFHFANYKLPDLRPDLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WB85</accession>
    <name>A0A6E8WB85_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Transducer of regulated CREB activity middle domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8WB85"/>
    <dbReference type="EnsemblMetazoa" id="ACON029610-RB">
        <property type="protein sequence ID" value="ACON029610-PB"/>
        <property type="gene ID" value="ACON029610"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_014546"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON029610"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033541"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008140">
        <property type="term" value="F:cAMP response element binding protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024786">
        <property type="entry name" value="TORC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024784">
        <property type="entry name" value="TORC_M"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13589">
        <property type="entry name" value="CREB-REGULATED TRANSCRIPTION COACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13589:SF15">
        <property type="entry name" value="CREB-REGULATED TRANSCRIPTION COACTIVATOR, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12885">
        <property type="entry name" value="TORC_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transducer of regulated CREB activity middle" evidence="2">
        <location>
            <begin position="144"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="194"/>
            <end position="385"/>
        </location>
    </feature>
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            <begin position="290"/>
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            <begin position="329"/>
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            <dbReference type="EnsemblMetazoa" id="ACON029610-PB"/>
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</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WCX0</accession>
    <name>A0A6E8WCX0_ANOCL</name>
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        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
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            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
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        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
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            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
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    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="9">Ngousso</strain>
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        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
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    <dbReference type="AlphaFoldDB" id="A0A6E8WCX0"/>
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        <property type="gene ID" value="ACON029804"/>
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</entry>
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    <name>A0A6E8WCY1_ANOCL</name>
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            <taxon>Pterygota</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
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    <reference evidence="6" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="6">Ngousso</strain>
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    </dbReference>
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        <property type="gene ID" value="ACON029101"/>
    </dbReference>
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    <dbReference type="VEuPathDB" id="VectorBase:ACMO_008799"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON029101"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039280"/>
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    </dbReference>
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        <property type="entry name" value="Cytospin-A isoform 1"/>
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    </dbReference>
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        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050540">
        <property type="entry name" value="F-actin_Monoox_Mical"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23167">
        <property type="entry name" value="CALPONIN HOMOLOGY DOMAIN-CONTAINING PROTEIN DDB_G0272472-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23167:SF69">
        <property type="entry name" value="FI18193P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
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            <begin position="1216"/>
            <end position="1321"/>
        </location>
    </feature>
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            <end position="68"/>
        </location>
    </feature>
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            <end position="181"/>
        </location>
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            <end position="266"/>
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        </location>
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            <begin position="318"/>
            <end position="327"/>
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        <location>
            <begin position="1063"/>
            <end position="1074"/>
        </location>
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        <location>
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            <end position="1085"/>
        </location>
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        <location>
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        <location>
            <begin position="1137"/>
            <end position="1155"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00009452"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblMetazoa" id="ACON029101-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1322" mass="145586" checksum="24A050C605C62E65" modified="2020-08-12" version="1">MSKLRQKIRNVFSWQHDDYTFEPVPESAQSSGPPLPLPPPKESKSNRFLKAGSSPSKAVATSSKGVGNRTAKINKKRLNQINIARNSSITSAQLNAAVPLVNIRRAPSDRSVLSEIDRQIVTGRPAAGSRPLLSRPITVTGGGGVGAPSSVSGRSLASKASSSRASSSVPSTSASQKDIVVVRRAPPAPVVVQNDRSLLRTIKKQQQEPSSERAVSADRSSIKRKPLLSGFGRQRGQGPSGSKHQSQPSVSSVSATGSGHADGGSVVPGAGLTGALSVNGGGGGLLKSAASASSLEFPVVSKPAHSSRGTGQQQRFNGSKEKLDHSSSHHQHHHHHQHLHHHQQQQQQQLHTQHQHPLGSHQPQHHQQSRTASAQMAQMATGATVSTNHLNKFPPGSGSSLVVSDDGGGLLLNERGVDVGSGLSASMEQLTAISFVGPEKASSGGAGRKEQQQQTSPSKSRSAELLQVHLEKLQSENRLLERKVHEMTSCQEELLLLRDEIVKLKASHEQSNSELHRLVNENESLRDRLKTVVQSPLSDSEKQQLIRNTQRLHSSAPASIALPNNMDAEGTPCVTPDWDKQSSSSEVAVACLQDKIIQMEETHYSTNEELQATLQELADLQSQIMELQSDNERLVEEKDVIFQSLCRQTEKLEDTRTQIGTLQKLLLREPNQQDVTPTDREQKLVDLLKSAQDERESFMLKQEELNAELNELKAIIDERSGEVARARGRISMLESSLDAANAEKKDANAQLMESKEDASVKLIEISRLTTLLENARAKIDELEQDRAKGDKTDLEELLDVARKEKDQLETQVASLQEQVSISQCEIQKLKDQLARLNEECKVVRNNAKCVISDLEYKNETVTQEKQKMATDFQQLQESINELQVQNKCLLEDKSQLETLLSETQKHLGETERQLMEKTDELNQETRLRKQEADEWEHFQSDLLMTVRVANDFKTEAQNAREKLALDNKALREKVRVLEQQIEQLNKLITTKETQSQSLITTVQQEMAVRRQQKSAIQRQDSRLSVKSLIESIENSAKQTKLNSDSRCSSSSSINSIPADANPATLSAKHSSVSSTNNNNNNTINNNEHDSISNNISKGHVNGSNNNDENNVIQIPVQPSAAMVQSGGGALPAKSPLREQQQPTVGSNVNSNSKPNASADTVMLMKKSSLITSNNGCNTTLNSAIISHKTMDYVRRNSYNDISERKDPLNALVKNGGSKRNALLKWCQNKTVGYRNIDITNFSSSWNDGLALCAIMHSYLPDRIPYDKLNQNDKRRNFSLAFAAAESVGIQTSLSIDEMCLQERPDWQQVMGYVTAIYKHFET</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9I3AX44</accession>
    <name>A0A9I3AX44_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Vascular endothelial growth factor receptor 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2023-03" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON008813-RB">
        <property type="protein sequence ID" value="ACON008813-PB"/>
        <property type="gene ID" value="ACON008813"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037366"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004714">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001641">
        <property type="entry name" value="Myoblast growth factor receptor egl-15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000373">
        <property type="entry name" value="Receptor protein-tyrosine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000384">
        <property type="entry name" value="Receptor protein-tyrosine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050122">
        <property type="entry name" value="RTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416:SF600">
        <property type="entry name" value="PDGF- AND VEGF-RECEPTOR RELATED, ISOFORM J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000615">
        <property type="entry name" value="TyrPK_CSF1-R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="17" id="KW-0732">Signal</keyword>
    <keyword evidence="2 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039946501" evidence="17">
        <location>
            <begin position="31"/>
            <end position="1427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="780"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="19">
        <location>
            <begin position="49"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="19">
        <location>
            <begin position="256"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="19">
        <location>
            <begin position="473"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="19">
        <location>
            <begin position="681"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="18">
        <location>
            <begin position="854"/>
            <end position="1224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1294"/>
            <end position="1396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1319"/>
            <end position="1330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1351"/>
            <end position="1362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="1371"/>
            <end position="1382"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="1088"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="861"/>
            <end position="868"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="888"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="892"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="1092"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="1093"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="1106"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for interaction with phosphotyrosine-binding proteins" evidence="13">
        <location>
            <position position="1232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00051243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000615-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000615-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000615-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000615-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008813-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1427" mass="161409" checksum="5319A1916772AA02" modified="2023-06-28" version="1">MVGQPATVILSVTLLAVCSVFQHSATFVSALDEGHIVREQDAANAHGAPEIDTVEEEIVLPKGASWNFTCKSAQPILWKSYAASFHWEPPNVYPVDFETDDPDKPYGSVLMLTDASAKQVGRYYCINVASYDEEREDELDDMVAEYLASTVYVYVNDPEHPLVPVSSPVFRVQQYDDFVIPCKPTHPEVEVELYNDLEGNLVETYEYSNTQGYKLSFNRLEEGGFYYCQVKDKPDQRIDFEVTIIEHSLTEYLTKPSIHSDTKDHVPLGQRIRLVCKVNIRAGVNLDMIWKVPPNLNPAVRDARVRLGSLKLNPVKDAAHREIASRELIIERATMADDGTYRCEVMDVNGHRNYHSFKLHVHDSGDDYVMLREENNLDDINVRRNVNGKTAPIDIVIEYRSYPANITYYWLKDDDIEVMAGHHGKYELTHSETIVKLRINDPSVTDTGNYTLFVLAGNAEKSHRIGVYVYAKPIVHMESKFVKPNEEVSFTCRSIGYPRPEISFMFLPCLEVPWRNCSASRTNKDKWDTPGGKGETPIAKSRVYTLTPKQPGVVYCRATNLEGSEITEADLLVSDLSDSVTLEKEQPQEAITVGDHVTIVCSALVYNYTKDITFVHNDKELQESDGVRHDYSPELYAWQARLDIAHVATEHEGPIYCRAKTMTGAVESRAFHVEVLEPVAPMLVSGKSNESLSVDLHDPLKLECDIVGTPDPKIVWLKDGEPVRPDENSNRVQLTKTTLIFEYLKTDDLGMFECRAENKMGTIEKYWKVDVRTAVVRKSVIYIILSLLLVLIVGIVLVSLFYCRKKKEVKAMKEAGIVNFEEGNLGVYNPELALDEQADLLPYNSEYEFPKERLKLGKQLGTGAFGVVMKATAQRIMVNEDETTVAVKMVKKQTDNEVMRALVSELKIMVHLGQHLNVVNLLGAVTKNIAKRELMVIVEYCRFGNVQNFLLKHRPHFIDQINQETGEIDSSIDKNQLRWSKCGYQYNSGSTQYVDPKKHLNSKGYVRHSGLQNMGMVDSCNTEVTAMTSVEVEDLNSVNSNEPLWRSNYGMDYKGPARTVNTTDLVCWASQIACGMEYLASRKVLHGDLAARNILLCDDNVVKICDFGLARSMYKSDNYKKKGEAPLPFKWLALECIGDNVFSTYSDVWAYGIVLWELFSLGKVPYPGMEANQELYNKLRDGYRMDKPQYSNQDIYDIMLNCWNVKPDSRPSFKDLKSRFNAMLPDDMRDHYLELNEPYLQMNAEKMERGDTDYLANLGPPEEPAPAAPNYVNGIILPLPPISEIRNDKDYLKMSRTKSESEESNFDFASFNSDRHSPTIRNNLDTSPPNGSKRHKKRGLPEEIPMLDGNRLSSTANGFNSDSETEPVSPKPRERTSKHNPEPEYINVKGSKVGGVGRAPLCDDELSQEAISNPGYIALSMVDEKRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9I3BCJ3</accession>
    <name>A0A9I3BCJ3_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Axotactin</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2023-03" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON029629-RC">
        <property type="protein sequence ID" value="ACON029629-PC"/>
        <property type="gene ID" value="ACON029629"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041874"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00109">
        <property type="entry name" value="Kunitz-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00110">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000459">
        <property type="entry name" value="Axotactin, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000100">
        <property type="entry name" value="Axotactin, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.410.10">
        <property type="entry name" value="Pancreatic trypsin inhibitor Kunitz domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002223">
        <property type="entry name" value="Kunitz_BPTI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036880">
        <property type="entry name" value="Kunitz_BPTI_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001791">
        <property type="entry name" value="Laminin_G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050372">
        <property type="entry name" value="Neurexin-related_CASP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15036:SF49">
        <property type="entry name" value="AXOTACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15036">
        <property type="entry name" value="PIKACHURIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00014">
        <property type="entry name" value="Kunitz_BPTI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02210">
        <property type="entry name" value="Laminin_G_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00131">
        <property type="entry name" value="KU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00282">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57362">
        <property type="entry name" value="BPTI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50279">
        <property type="entry name" value="BPTI_KUNITZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50025">
        <property type="entry name" value="LAM_G_DOMAIN"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039894303" evidence="5">
        <location>
            <begin position="28"/>
            <end position="1942"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1720"/>
            <end position="1741"/>
        </location>
    </feature>
    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="8">
        <location>
            <begin position="43"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="6">
        <location>
            <begin position="156"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="7">
        <location>
            <begin position="353"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="6">
        <location>
            <begin position="395"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="6">
        <location>
            <begin position="573"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="7">
        <location>
            <begin position="754"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="6">
        <location>
            <begin position="1000"/>
            <end position="1172"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="7">
        <location>
            <begin position="1173"/>
            <end position="1210"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="6">
        <location>
            <begin position="1216"/>
            <end position="1431"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="7">
        <location>
            <begin position="1432"/>
            <end position="1470"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="6">
        <location>
            <begin position="1473"/>
            <end position="1667"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1867"/>
            <end position="1942"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1905"/>
            <end position="1918"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="1440"/>
            <end position="1457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON029629-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1942" mass="218940" checksum="73F70909D305F265" modified="2023-06-28" version="1">MTYHAASVLLTLCLTLAPIPYDRCVLAIPTNAEPPKEIVYPKCTGPGEPGQCQTFDYRYRFEQTINNCTQFIWGGCGGNLQNNFETYEQCMQQCANETQTPQPPVPLTTTVDPSSTSVRTQTQEAWSLHSSSSTLAPVPDELRGSELTFKETGYEKTFMFAKNNTFIQMDGETIQTFQLRLCREISFQFRTRLPHGLLVYHNVKTPAGVKLDPYALYVIVEKGQLKVVHVFGNHSTSVTVGEGLNRDEWHSVMVRIDVHGARLIARVDNNKEEVYLKGLNHETNYGVSINLMSVVLVGGLSSEEKLHGVKYIIESFVGCIRNVVLSSGKAASDLLPITPLIATKHENVKEGCRNKCHSRQNLCFTGSRCINHYYDISCDCFGTKYEGEHCDIYTATVLTLRGSSYVSYRIYDWKDRVHSPITRISMMFKTNYDDSALFYASGESLKPQYIAASIKNHSAYIEMDFGDGVMSATLGQDLTRHYWHNLTILHEHDKVIAILDDHVRILDLLGPIHNLLFDPEIYFGGGPNLSKRKGLASSNNFVGSLKYVFYNDVSVLFELKKGNPKVHYIGVLQEEFYEANVEVIPITFSYAKSHIWWPMNNPDTLSLKFDFKSSKATAVLAYSEVKTSEGNGFWELRLATDRLSFDLCPDVKSNITHTTYIRIENPTSWHSIQLTYERNDIKFVVDYRHSVTQMYGLSFNIGDKLIIGSSLKAAASGLVGCLRDLKINGVEIEPRYLVKSERVVGDVSLDNCKYVDPCKKPNTCEHGGKCAVKDDGIICDCKDTGYIGKNCHFTKFRKTCEELALLGYSKSDVYSIDIDGNGVFPPARVKCDFQSLANATKTIVEHNLPSQVDVRSATDEDFSFNIQYREFSAEMLQELISHSLYCTQYIKYDCIKAPLELHSSTWFKSSSNNNTVDSLGEVKRGTCPCAIGKNCEDLNHSCNCDANLNRWLSDEGYYKEPPNLGITQMYFLQQKNLDEESQGRVTLGPLECVETNTQKYVVTFTTSQSYIEVPGWRKGDIAFSFRTTGEKAILLFQPPIRSNYPSFMVALTGDYQLTFNFTLNTGTPKTLVIDTNRKLNGGEWHKIWIDYNEHHVRFMINTDTRMVDLQPEEEFGPFEGSMFIGGAPTEISKKYTVKQGLIGCFRGLVVNGEILDIYSFMSVHLSEIIKDCKPSCVPNPCKNGAYCRELWSTFECVCQNRWAHLGQHCETNINEDALTFISRESYLKRNYLSDPEDMRDEREKLRAILNSSLLVNLRTYDRHAFVLYANDHYNNFIHLYLTDQSEMVYLYNYGSDIVNLTIEHSELNNARSIQVAVIRTETNTTLYVNEKNVTVERGFLLLDEYSNKPWTNPELEVLSPHRPPAPPTEYFQFNIGGYDPANLLRPNQDTTELEGYIGCIRGLKIGENLISLREMATQNNAHVTDGVLNGCQMKCDAEPCKNGGICTENFVRQESTCNCEHTSFLGEFCSEEKGASFSGEATLLRRLALADTVGSVRLQLAFSSNDMRRANRVMLLLQSRNDRSYYLMVAITAENHLYIEEDREGATYAARIEGNFMDDARHSVYYTRHGDEASLLVDRAQIPIRAVANKPLVPVPDPGANHVQIGGVNTTDPRFAVYKSYDGCLSNIFIQVNNETMKPLEEYMLFTKSEAETITVINSQGVRSAQCKQFDVIQKLTPFNEPALNMTSVIDKNWVVDAPTRLPYKAVYFDPSTKEEKTQVVFITLTAIFVIIVVCCLIEVYRSDREYRKRIERQTDEDIIWSKEQAAKMQIETSTTNVKGFSYKSIPSDEKKDNGAKPLVGILKNGSVTAASEKPSPVATVAAAPVESLPQSNGTAETGGIRDSQLLDHELQWESLAAEENEALLKSAQHPTEQQSRHGESNGVDGPRARIANGNHHHHHRVTDLDDPSDGHEEDSELTDNPVPPQPEQQQQSNPEGTESRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9I3BDE4</accession>
    <name>A0A9I3BDE4_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="5">DEUBAD domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2023-03" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_040232231.2">
        <property type="nucleotide sequence ID" value="XM_040376297.2"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON030694-RA">
        <property type="protein sequence ID" value="ACON030694-PA"/>
        <property type="gene ID" value="ACON030694"/>
    </dbReference>
    <dbReference type="GeneID" id="120955428"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030294"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002020">
        <property type="term" value="F:protease binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21865">
        <property type="entry name" value="DEUBAD_NFRKB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2430">
        <property type="entry name" value="NFRKB winged helix-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044867">
        <property type="entry name" value="DEUBAD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024867">
        <property type="entry name" value="NFRKB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025220">
        <property type="entry name" value="NFRKB_WH_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057748">
        <property type="entry name" value="NFRKB_WH_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038106">
        <property type="entry name" value="NFRKB_winged_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13052">
        <property type="entry name" value="NFRKB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13052:SF3">
        <property type="entry name" value="NUCLEAR FACTOR RELATED TO KAPPA-B-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14465">
        <property type="entry name" value="WHD_1st_NFRKB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25793">
        <property type="entry name" value="WHD_2nd_NFRKB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51916">
        <property type="entry name" value="DEUBAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEUBAD" evidence="4">
        <location>
            <begin position="56"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="438"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1059"/>
            <end position="1086"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="2027"/>
            <end position="2053"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="450"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="2027"/>
            <end position="2051"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON030694-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="2142" mass="231755" checksum="06A12931674B4456" modified="2023-06-28" version="1">MGRRFVMDKKSSTETMVKIYKATENVYASSSGSDSDESNASLLERSSLLSNKFMLPKDLSENGNIFNEFFSYSLWDALPNATQQKLTKYLPVAKDDFSSTTKIVHSLFSRELNRFYKEPMESFRLNLSTGNYRVDIARLQQNLAKSTTKDRRMVELQRLSRLAQNVMISREAALFSELQFISNHALPSCGRVMDNYRKRRQREYDFEAPSVMAKCQAVATKRYLSEIVSISEEAGLSLSLSDEEECLQALPTAPIRKQRRILGYNVQSGEQSCHLNRTIGHPNLSGASGFTNILSKKSALVNTSNGMGGNTFDGTIFNAPKLFIVTEDHYRKLLVQHRKRKMEEPNHPELNLDGVKLKDVVSRTQIAAGYRRILPLPKVYISDSAKENPIGIKARIKSQKLFRMHRVSPDQADLKHSVGLNLEERGINITEIKVEPSVDADQKSKSVLPNDSSESNSTDNELHHLALKHDEFIEGTRAASNHDKTKENGEDPINTVEKLKPFSEHVLDETELSIKPTAVTSLNPNVLNSVAQSDVPKKASDTSTGDNNSMNLLLSNGTHACFLSVVRDLFCSNPDHRCTMSELMQKLEAWTKSPVAERNDWFEQYNKNGDWDSTLQSAVKFLTGEFQNQPEDFVPYIEHKIAINILQWIGASRDADSRLVPLCTYWQNRKQEMNKQTNMPTITANNIIGLNNTLPIDTNMHVTVSPKNKNKTNIGTKSLASSAYSSTSSSILLPSAGEVSIGFNESSVSSLSSMTILADEDGSASERSVTPPPPLFPTDWSVRKATDEEIQRFREQEKCRYENPHMAFTYKQHSYDSVVGPVKGIYTQAPGISKARGHSMLVANRPNFVTILTLVRDATARLPNGEGTRADICELLKSSQYISPTATDQILQTIVSGALDRMHTEHDPCVKYDAKRKIWIYLHRNRTEQEFERLHMQYQGLSKHKKSIVRKSMRQSRESLTTATSKISNLAKQHQIDDNGPSRDNVASVSSAVEILRDNTTEIINVMDSGTDLSAATSVINIPTTIVGNTVTVARSTTSLLKKQVPLTVMDNSIVQQSCVDTRNENRSASNSPSQTDRTKKSINTTMQDHQLLDSGSDTIYKSSSKLYNAVTLITSSGNNVRTIQIPKSSISLSNRPDVASNHSTVTGNEVLTSELSAHNTSPQSPKKIIVSAVVSAAKTMVASTAISPSTDFSLNNCVDNLQKLNTKPILVQSAGTASPIITPSSCSFSKDAIKISSAIGGTIVSIRQVNNTNLGTLGQKNQTFACAQSTSLLSPQLSTQAKRPQISMNSSSIATTSAANAIRTIKGGTIISAAIEKPNYSSSVTPSTSKQSSISAPMLQSNPATTVLSQKMMLSSARAYASSAGESSNVPTVVAIKPTNSTSITTVTETGTNRPIIRPTANLFSTITLTKGQQSVLTPAKQQQIIQNLLLQNQKQSIITKQIVCTTDDAKQGVGVGSPFQLESPTAVPASFKMQHNSTSSVKPLSQQIVKITPTRAKTHCISTIRPVSTIVTSSNSPGHPSSSIVNQTSPGVKMIKMHPSTMLTSIEPQGMLNASTLCGANTVTISGTNNIGSPASTPNSTMRVLKTATGTTTVIKTEVTPSGLSEKLLSQGTRHHVLDKHRNDLTAHVGSKVIVTPSISTGQLIPLESLLQKQGVTPTTNSSTGGLNTFLKITGTNKTGQQQFLQFTTSSNAASSITSTSASNVLSNSGTTSLGSVRQQYTILPQSRNIISVASTSRTSRKNNIPLTIVSSQDSNESLPSAASLIVGNKKGNVTEVNTIDDTVPSPPVHSPSEKQIPQVKLLTTAQSPKSFSSGAITSTTTIQPQNNISKTKTSLDNTDLLNAKIIGVRNIASTKLKGTSSLSLMNTGGLNIAHIGGKPVIIANNAAIGTNQGITVSTSHLHNNGVNKGTPAGTMLLTSNTNSISPALEQQNSNDMNVPGLPSTARRENACTNSVIPGQIQTLMIRNNLLKVQSVPPTALNKLSQHTTISAGSVGASGAGSGVKTSTTPITVCAGRCTTAQSRQQQQTQEAQTQYPQQTPQKQHQSKQLGSAGSAILFTPHVAANNKPLGAMSMEDKSFVKIQSSSTEISNNADGGSQKVQSQRIVLSQPTIASDLNVAGNALNFKRLKVIPISKQSKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9I3BDG0</accession>
    <name>A0A9I3BDG0_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Calponin-homology (CH) domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2023-03" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Myofibril</location>
            <location evidence="2">Sarcomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the nesprin family.</text>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON030705-RB">
        <property type="protein sequence ID" value="ACON030705-PB"/>
        <property type="gene ID" value="ACON030705"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032277"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034993">
        <property type="term" value="C:meiotic nuclear membrane microtubule tethering complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005640">
        <property type="term" value="C:nuclear outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030017">
        <property type="term" value="C:sarcomere"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008285">
        <property type="term" value="P:negative regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007097">
        <property type="term" value="P:nuclear migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006997">
        <property type="term" value="P:nucleus organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd21241">
        <property type="entry name" value="CH_SYNE1_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00176">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000378">
        <property type="entry name" value="AGAP009554-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000402">
        <property type="entry name" value="AGAP009554-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000413">
        <property type="entry name" value="AGAP009554-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000414">
        <property type="entry name" value="AGAP009554-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000420">
        <property type="entry name" value="AGAP009554-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000435">
        <property type="entry name" value="AGAP009554-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000459">
        <property type="entry name" value="AGAP009554-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000468">
        <property type="entry name" value="AGAP009554-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000471">
        <property type="entry name" value="AGAP009554-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000576">
        <property type="entry name" value="AGAP009554-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000033">
        <property type="entry name" value="nesprin-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000037">
        <property type="entry name" value="nesprin-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000169">
        <property type="entry name" value="nesprin-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000171">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000195">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000188">
        <property type="entry name" value="Uncharacterized protein, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000222">
        <property type="entry name" value="Uncharacterized protein, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000230">
        <property type="entry name" value="Uncharacterized protein, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000286">
        <property type="entry name" value="Uncharacterized protein, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="32"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001589">
        <property type="entry name" value="Actinin_actin-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047290">
        <property type="entry name" value="CH_SYNE1_rpt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012315">
        <property type="entry name" value="KASH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052403">
        <property type="entry name" value="LINC-complex_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018159">
        <property type="entry name" value="Spectrin/alpha-actinin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002017">
        <property type="entry name" value="Spectrin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057057">
        <property type="entry name" value="Spectrin_SYNE1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47535">
        <property type="entry name" value="MUSCLE-SPECIFIC PROTEIN 300 KDA, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47535:SF1">
        <property type="entry name" value="NESPRIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10541">
        <property type="entry name" value="KASH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00435">
        <property type="entry name" value="Spectrin"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25034">
        <property type="entry name" value="Spectrin_SYNE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01249">
        <property type="entry name" value="KASH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00150">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="40"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46966">
        <property type="entry name" value="Spectrin repeat"/>
        <property type="match status" value="36"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00019">
        <property type="entry name" value="ACTININ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00020">
        <property type="entry name" value="ACTININ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51049">
        <property type="entry name" value="KASH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="9 15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="topological domain" description="Cytoplasmic" evidence="14">
        <location>
            <begin position="1"/>
            <end position="16329"/>
        </location>
    </feature>
    <feature type="topological domain" description="Perinuclear space" evidence="14">
        <location>
            <begin position="16351"/>
            <end position="16379"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="17">
        <location>
            <begin position="148"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="17">
        <location>
            <begin position="313"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="domain" description="KASH" evidence="18">
        <location>
            <begin position="16321"/>
            <end position="16379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="7889"/>
            <end position="7915"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="7949"/>
            <end position="7991"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="8081"/>
            <end position="8223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="8335"/>
            <end position="8356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="8528"/>
            <end position="8608"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="9051"/>
            <end position="9112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="9138"/>
            <end position="9157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="9282"/>
            <end position="9302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="10950"/>
            <end position="10986"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="11106"/>
            <end position="11210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="11264"/>
            <end position="11293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="11361"/>
            <end position="11488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="11516"/>
            <end position="11564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="11605"/>
            <end position="11648"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="11776"/>
            <end position="11803"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="12046"/>
            <end position="12116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="12386"/>
            <end position="12430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="12869"/>
            <end position="12894"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="13041"/>
            <end position="13065"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="13361"/>
            <end position="13457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="13552"/>
            <end position="13580"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="13685"/>
            <end position="13739"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="13823"/>
            <end position="13868"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="16250"/>
            <end position="16308"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="2282"/>
            <end position="2309"/>
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    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="2457"/>
            <end position="2491"/>
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    </feature>
    <feature type="coiled-coil region" evidence="15">
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            <begin position="2601"/>
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    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="2928"/>
            <end position="2959"/>
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    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="3294"/>
            <end position="3321"/>
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    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="3597"/>
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    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="3843"/>
            <end position="3870"/>
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    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="3995"/>
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    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="4104"/>
            <end position="4131"/>
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    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="4598"/>
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    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="4769"/>
            <end position="4835"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="5166"/>
            <end position="5201"/>
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        <name type="scientific">Anopheles coluzzii</name>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
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            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
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            <taxon>Anophelinae</taxon>
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        <scope>IDENTIFICATION</scope>
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        <text evidence="11">Histone methyltransferase that specifically trimethylates histone H3 to form H3K79me3. This methylation is required for telomere silencing and for the pachytene checkpoint during the meiotic cell cycle by allowing the recruitment of RAD9 to double strand breaks. Nucleosomes are preferred as substrate compared to free histone.</text>
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            <text>L-lysyl(79)-[histone H3] + 3 S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl(79)-[histone H3] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
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        <text evidence="11">In contrast to other lysine histone methyltransferases, it does not contain a SET domain, suggesting the existence of another mechanism for methylation of lysine residues of histones.</text>
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    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1475"/>
            <end position="1492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="1500"/>
            <end position="1509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1548"/>
            <end position="1560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="1585"/>
            <end position="1603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="1636"/>
            <end position="1661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1742"/>
            <end position="1755"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="1763"/>
            <end position="1778"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="1831"/>
            <end position="1869"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="1871"/>
            <end position="1882"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1955"/>
            <end position="1965"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="1966"/>
            <end position="1988"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="2006"/>
            <end position="2021"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="2124"/>
            <end position="2135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU271113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003282.P212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="2141" mass="227841" checksum="D09DCE833A54E31D" modified="2025-10-08" version="1">MATPNYKELKLQSPAGAEPFLYNFPFSTVMGTGHDSGAELIENVRWVCEDMPEIKSAIEEIDLNNLDTNNYDAMKNLCDRFNRAIDSVAALEKGTSLSNQRFTYPSRGLLKHILQQVYNQAVVEPEKLNQYEPFSPEVYGETSFDLICQMIDQVKITADDVFVDLGSGVGQVVLQMAASTPVKVCFGIEKADVPSKYAEGMNTTFKLWMRWFGKKYGDYELIKGDFLADEYREKITSATIVFVNNFAFGPNVDHQLKERFADLRDGARIVSSKSFCPLNFRITDRNLSDIGTIMHVSEMSPLRGSVSWTGKPVSYYLHIIDRTKLERYFQRLKTKGTENHTDGTSGGGSGSHSTRSSRSRKDNNTNHHHHHPKVITNDDSTSESDTDVVGPTTRKAWSDWNSGKEGKTSPSEEENNNSPVLRNGRIPVATKKRRKITRTKAAGKKAELAAASAAAAAAAAAAAAAANRDMGVGTSAASAAAAAAAAMAGGKKRGRVKGKGRQRRPLNIAGLDLLHNETLLSTSEQMIGKRLPPAPGCVDQQLTSLAGDMQHNELDIPEAPSETPYALQILLDLYKTQFMKTIEAMRKPSYKDNVQQQFDREKERNQRLMNRAGQLEKQIKVLIDDSVALLKARMNELGISTTSQNDLLCKAKEIVGRHKELQVMAAKLQNQVNVIEQEQKRLVMQHLTKLTVEQQQQHQQQQQHQQLHPHQPYIKQEDAELTSSSSNELVLKAIASTLSQRKKLYAQVSNLETELNLIEKLTEERKSMVVGLSAAAPNSNHNSASSTAAAAAAAAATSTIISVARATEVRDRELYGHAGQLHPATIGGGREREHIHPDGTTTSKHTSDPVRTLPAGAAAGPVGVAGSAAPLPPPPMAGVGSASVTVPSTPTKQGSGGGSSRSAQRKSRENRTRSQEWPEIPDIGKIEENNPEILAQKILETGRQIEAGKLLAAGKHASKERGSEGKLAPVVTAGHGSSAAAGTAAPQHIAHPPTVGAPAPQQQPYPHHHQQPHSQPSQPPVHPHLHHPDTALMPAPASTINKAHHHHRSNSGGPSGAVPPSVPTGGGSLPKCYVPGTASNEHHSGGGRNAAEGASGTGRGGSSGGGGGSGKLQDSHKVVNFEDRLKSIITSVLQGSPKTGNTASSAPAPVSLTVTGPPPAGPSPGAGHRDAHHRSGSGGHQPLTMEPAGSPLKATSASGAGYGSAAGPGKTTVYLQSSPGAGSHHPHQMVVAQDMSARSSSGGRGPSPSAHNPAHQQQIHPHQVSQSQYQQQQQLLHHPHHPHAQQSLHHQQQQHQHYQMQQQQQHQHQQQQQQGMLNVITSGAHHLNASTSISTSPVPTSPYKMHPTGPQASSIAGSASTKISPSSKYPYQKGGPVAGMSHHSPGSSALSTHQQIIQQQERERAMLYAAAAHGGGLPIDHPHHPLHQHHHRGMPPSLVDGKMLEFKAPENFRYDPRASGPSGAAGNGPPMLDTSAVSLQSHSRSSSTNSLDSIPAADYGPASAGASAVGSGGARYGGQQQQPIPLVTHSPGVGSQGSGPQHGGNNSRPGSTSSQPDYTQVSPAKMALRRHLSQEKLTHPSAGGPAAGGPVSGGPGAGGSGGGLGTVKTIGDLVNGEIERTLEISNQSIINAAINMSSHQQQQQQSSAASGNSSAPPASGNNTVINTHVQRPERVSIRLLEEAGHAAAGGPPPPGSSGGTYSPISRPGSVGDSGSKSPVHHLHGQSNLASLVQVSAYNSKNQKGAGTTAVPSTIVSPRGGGSSQQQQHHQQYSTAPGSGAAGGPGAVYQQSTSRGHDRHHSGEPMPYMALPRADMKPYLESYFTDEHNKRQQQLHQQQQQHQQHPQHQQQHLQHPVQSPSPSAMSASAMGLHHHQQQQMHHQHAPLAMHRASHPVDLHRGSVVMNEPGMLSRSRGEMIPMDDNRMDRLNGGPPLEGLAASLQARVIATLKIKEEDEERHRRDLSIHHSTSGTINSSSNSINSTNSNIHGGSLQIVQTAHIKSEKYTSSSSSSSTSSSSSTSSHHHHALKRTSPIVEHPAGTRPPKMLYTTSASAGGMDCGPDADMLHVPRGAVPGSSVVGHSAVRGGLLVAPPLVMSPEINSLSSVVDDGRHHHQLHVRHNHHSRNDVDAHPNERRRNRIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8UYM2</accession>
    <name>A0A6E8UYM2_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Palmitoyltransferase</fullName>
            <ecNumber evidence="7">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:36684"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the DHHC palmitoyltransferase family. ERF2/ZDHHC9 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8UYM2"/>
    <dbReference type="EnsemblMetazoa" id="ACON000567-RA">
        <property type="protein sequence ID" value="ACON000567-PA"/>
        <property type="gene ID" value="ACON000567"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_014268"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000567"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042638"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12349">
        <property type="entry name" value="ANKYRIN REPEAT AND LEM DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12349:SF2">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="42"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="144"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="181"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="9">
        <location>
            <begin position="98"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="260"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="263"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="274"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="299"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000567-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="330" mass="37563" checksum="60F7404B16B02C33" modified="2020-08-12" version="1">MPKCDVKTRYIPATFAWTLLLSTTFLFFWYPCRQFYIHRHPWVPAYQAVITFFVIANFTLATFMDPGVIPKAPPDEDREDEFRAPLYKNAEINGITVRMKWCVTCKFYRPPRCSHCSVCNHCIETFDHHCPWVNNCIGRRNYRFFFFFLISLSVHMLSIFSLSLVYVLQKEKDKLTEVEPIVAMILMAIVTLLAIPIFGLTGFHMVLVSRGRTTNEQVTGKFKGGYNPFSRGCWNNCCYTQCGPQYPSLLKPQKYVARRSNKENQSISTITNDGGPGGGGGDAAGSGRRHMPTGGPGSQQQQQQQQQQQQALNQGGGIYDNNRHTQLTEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8UYN5</accession>
    <name>A0A6E8UYN5_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">AMP deaminase</fullName>
            <ecNumber evidence="9">3.5.4.6</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">AMP deaminase plays a critical role in energy metabolism. Catalyzes the deamination of AMP to IMP and plays an important role in the purine nucleotide cycle.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>AMP + H2O + H(+) = IMP + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:14777"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="3.5.4.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; IMP biosynthesis via salvage pathway; IMP from AMP: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the metallo-dependent hydrolases superfamily. Adenosine and AMP deaminases family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.6" evidence="9"/>
    <dbReference type="RefSeq" id="XP_040239577.1">
        <property type="nucleotide sequence ID" value="XM_040383643.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8UYN5"/>
    <dbReference type="EnsemblMetazoa" id="ACON000577-RD">
        <property type="protein sequence ID" value="ACON000577-PD"/>
        <property type="gene ID" value="ACON000577"/>
    </dbReference>
    <dbReference type="GeneID" id="120959892"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_003237"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000577"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040059"/>
    <dbReference type="UniPathway" id="UPA00591">
        <property type="reaction ID" value="UER00663"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003876">
        <property type="term" value="F:AMP deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046033">
        <property type="term" value="P:AMP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032264">
        <property type="term" value="P:IMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01319">
        <property type="entry name" value="AMPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.800.20:FF:000001">
        <property type="entry name" value="AMP deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000035">
        <property type="entry name" value="Probable amp deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.800.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006650">
        <property type="entry name" value="A/AMP_deam_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006329">
        <property type="entry name" value="AMPD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01429">
        <property type="entry name" value="AMP_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11359">
        <property type="entry name" value="AMP DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11359:SF0">
        <property type="entry name" value="AMP DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19326">
        <property type="entry name" value="AMP_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001251">
        <property type="entry name" value="AMP_deaminase_met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00485">
        <property type="entry name" value="A_DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00054146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR001251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000577-PD"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="782" mass="89066" checksum="20CC05BBF7E946BB" modified="2020-08-12" version="1">MSARGVLLKCDMNSSFDQPDPAIMSMSPRSPAILLNDFFSPEVQGNESPAGHAGAGGGGGGGVGPAGVLSGALSLSQEDIVGVLPNEISAPYEVPQFPIEQIEKKLQLQRQLNAKVMEQDRHSIAPEVHPDEQLPFDEHDFVAHFQRVSISGEDTSGVPLDDLERASTLLVKALELREKYMRNSHQAFPQTTARFLKSTHSSTITHQERKSIADHPVNPPQSTQSPWVVEIPEDLNYSIRPVHGVFEVFPTVESTEPLPYQYTRLPDFVQDMQMMCSMIADGPLKSFCYRRLSYLYSKFQLHVLLNELRELASQKAVPHRDFYNIRKVDTHIHAASCMNQKHLLRFIKKTLKNSADEVVTVTKGTPMTLAQVFQSMNLTTYDLTVDMLDVHADRNTFHRFDKFNAKYNPIGESRLREVFLKTDNYLNGKYFANIIKEVASDFEESKYQNAELRLSIYGKSPDEWYKLAKWAIDGNVYSDNIRWLIQIPRLYDIFKTNQLMSSFQQILDNVFKPLFEATNNPSKHPEIHTFLQYVIGFDSVDDESKPENPLFDGDVTPPADWTDVENPPYAYYIYYMYANMTVLNHFRAARGMNTFVLRPHCGEAGPVQHLVCGYLMAENISHGLLLRKVPVLQYLYYLAQIGIAMSPLSNNSLFLNYDRNPFPEYLARGLVVSLSTDDPLQFHYTKEPLMEEYSIAAQVWKLSSCDMCELARNSVLMSGFPHKMKQHWLGPNYTREGVAGNDITRTNVPDIRVAFRYESLLDELANIFKVNNEQKMQYASQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V061</accession>
    <name>A0A6E8V061_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Positive regulation of apoptotic process</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NIP3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8V061"/>
    <dbReference type="EnsemblMetazoa" id="ACON001411-RA">
        <property type="protein sequence ID" value="ACON001411-PA"/>
        <property type="gene ID" value="ACON001411"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009340"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001411"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038677"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097345">
        <property type="term" value="P:mitochondrial outer membrane permeabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043065">
        <property type="term" value="P:positive regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010548">
        <property type="entry name" value="BNIP3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15186:SF5">
        <property type="entry name" value="BNIP3, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15186">
        <property type="entry name" value="RE48077P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06553">
        <property type="entry name" value="BNIP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="149"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="32"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="32"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="42"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001411-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="184" mass="20795" checksum="6530DA00BC84637D" modified="2020-08-12" version="1">MLAGAVGTKSPDRTTPLAFASVEEYIRLLREAQRDESKESSRVASLTSTRKNSPRGSPKSPPNSPNTELAATEEDLRNVYINYVNKDGDIVKDTDWVWDWSSRPDQQPPKDWKFEHPNQTQEERNKPIHAGYSIRQVRVGKNSLFSREFLYSIVLTNVLSLLLGAGIGAWLQKRGLLLTRVSIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V092</accession>
    <name>A0A6E8V092_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">CBS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="4">AMPK is a heterotrimer of an alpha catalytic subunit (PRKAA1 or PRKAA2), a beta (PRKAB1 or PRKAB2) and a gamma non-catalytic subunits (PRKAG1, PRKAG2 or PRKAG3). Interacts with FNIP1 and FNIP2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the 5'-AMP-activated protein kinase gamma subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8V092"/>
    <dbReference type="EnsemblMetazoa" id="ACON001446-RE">
        <property type="protein sequence ID" value="ACON001446-PE"/>
        <property type="gene ID" value="ACON001446"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002448"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001446"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040820"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031588">
        <property type="term" value="C:nucleotide-activated protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019887">
        <property type="term" value="F:protein kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04618">
        <property type="entry name" value="CBS_euAMPK_gamma-like_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04641">
        <property type="entry name" value="CBS_euAMPK_gamma-like_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050511">
        <property type="entry name" value="AMPK_gamma/SDS23_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780">
        <property type="entry name" value="AMP-ACTIVATED PROTEIN KINASE, GAMMA REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780:SF35">
        <property type="entry name" value="LD22662P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0129">CBS domain</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CBS" evidence="7">
        <location>
            <begin position="450"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="7">
        <location>
            <begin position="531"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="7">
        <location>
            <begin position="604"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="7">
        <location>
            <begin position="678"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="737"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="43"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="118"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="136"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="224"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="284"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="741"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="753"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="776"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="821"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="850"/>
            <end position="864"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001446-PE"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="888" mass="97429" checksum="2988D18529BF1BE5" modified="2020-08-12" version="1">VSDDGATGSYDDGATTRRGRFRVSAPSTGPPETTVGRFRLIPQTGNYGPTSPFLQRGRFSVIPEEPQNSPGASALTPPIITTTVAPRQGSPDWDFDDDKSVTGGSGGSGPGAKRPSTAPATSGESSTRASKKQKKSEKEAKKQEKEVMKQKREEARRLEKEAAKLEKLNRKHESLSRSSERVGSGARSGSLERRRSGEEAPVLNQSTVHGIASPNRRPTIFDVFRPRKGSDSKKKKDDGSKSGSDKDSTGMGSGPISTSGGIMNSMKAALHVGGRHSHQHQQPAGATGAAATAGTSSKVRDGSAHPHAGSDAQYYHTVTAVRRADVGKSPMTKVMDLFRHRSNSAVSEADKRKAKAAAQHQQQLAVQSAHMRRASAELAERRRASLGATRGLRADGTLDPYHAAILFRDSRGLPVADPFLEKVPLSDLEEDESQIFVKFFRFHKCYDLVPTSAKLVVFDTQLLVKKAFYALVYNGVRAAPLWDSKRQEFVGMLTITDFIKILKMYYKSPNASMDELEEHKLDTWRKVLQEDVKKLVSIGPDASLYDAIKMLVHNRIHRLPVIDPVTGNVLYILTHKRILRFLFLYINELPKPSYMQKTLREVRIGSYNNIETATEDTSIITALHKFVDRRVSALPMVDSEGRLTDIYAKFDVINLAAEKTYNDLDVSLKKANEHRNAWFEGVHHCTLDETLYTIMERIVRVEVHRLVVVDELKKVIGIISLSDILLYLVLRPSGDGIGDSESLRATDPRLMKAGSPAGGSSSSKLQATKRDSSNESIEEEKETTEEDAEQEQQDAAAHKTETTITPATPSNDEDGQKNEAEVEVETADDAVPELAVEELAEEAKEDPDDTASTPVDRSSANDSPVAQEMLATDVPLQSVQREVGLVSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V4A2</accession>
    <name>A0A6E8V4A2_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Oxidation resistance protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the OXR1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8V4A2"/>
    <dbReference type="EnsemblMetazoa" id="ACON001751-RA">
        <property type="protein sequence ID" value="ACON001751-PA"/>
        <property type="gene ID" value="ACON001751"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005654"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001751"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034408"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00118">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.350.10">
        <property type="entry name" value="LysM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018392">
        <property type="entry name" value="LysM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036779">
        <property type="entry name" value="LysM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006571">
        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354:SF62">
        <property type="entry name" value="MUSTARD, ISOFORM V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354">
        <property type="entry name" value="NUCLEOLAR PROTEIN 7/ESTROGEN RECEPTOR COACTIVATOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01476">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07534">
        <property type="entry name" value="TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00257">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00584">
        <property type="entry name" value="TLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54106">
        <property type="entry name" value="LysM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51782">
        <property type="entry name" value="LYSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51886">
        <property type="entry name" value="TLDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LysM" evidence="6">
        <location>
            <begin position="152"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="TLDc" evidence="7">
        <location>
            <begin position="1006"/>
            <end position="1167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
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            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="411"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="444"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="557"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="623"/>
            <end position="649"/>
        </location>
    </feature>
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        <location>
            <begin position="710"/>
            <end position="751"/>
        </location>
    </feature>
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            <begin position="772"/>
            <end position="798"/>
        </location>
    </feature>
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        <location>
            <begin position="812"/>
            <end position="832"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
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        <location>
            <begin position="23"/>
            <end position="43"/>
        </location>
    </feature>
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        <location>
            <begin position="44"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="221"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="263"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="456"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="484"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="568"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="585"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="785"/>
            <end position="796"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00040604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001751-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1167" mass="124777" checksum="74007763A3EC09EF" modified="2020-08-12" version="1">MTTPSRDCSPKQRTFSGAGSGGFSAHTSSSSSLASSTAGNANSIESSLSFASVSGSPTKLSLFGSNKMSTTGSPPTLPTGPGPSRQRSLRDRLREGITGSLTWHKSRSVDHGLAAPFDLDSLRSKVEGRFESIDRLSKDGEKKRSMPTIPTITYTVKDRDTLTSVAARFDTTPSELTQLNRLASSFIYSGQQLLVPDKNAKASADGESDASTERSSNSPTDGRRKGSQEDLSQDEKDLLEGLRPGSPKPGHIERVASPNTQSGGAGGGGGGGGTAGTAAGGADDDDPVTIQRFLKINVRHITDGQGVVGGVLLVTPNAVMFDPNVSDPLVIEHGPESYGVIAPMEFIVNAAIFNDIAHMRVQGATGGNETAEKAEIYHPVPVEKPCSRCDTHSPGKDSLLVKDETFPELGVGSVEGCDDQESICSSNERDGDAFPKAFERDLVTPNNLQRLHGDSTESGGSSVEPKASSTEPPLFLSYDRDSSQSHTPNSLDHSAGASNEQQQQQKQAGDKTDQTKTNSILEDPTMRSLEERRRSLLDQHWAIPSKDRLKCYCDQSSSRRSLDDEEAASTGSGATATSSSTAGAGSIGGGATGGAHGAHHAPIAPIAEAAGDGQLVKQSCHDSGIDIRDPAHGSSLTTGATGGSGSSAASSVASIPIVAPIAAKKVYSDADIVLSADWVPPLTIAPTHLHDSSPRSSSSLVSSAHQSTLSTLTSDAGGRKKTSSVSFSVDEHDAHGATGPAGSGSLDKGGETKKNKMLKRLSYPLAWVEGLTGEGGSSSHKSDSIESAPNTGDSNQSVFSKVFSRRSSIGTFIRQHPSEGSANKQKPAPPKLDYRSMVSIDDMPTLFVSFDKLIPRPARACPDPPMYLRLRMGRKIGKNTPLPTTVMSYGKNKLRPEYWFSIPKNKVDELYRFLNAWVQHLYGELDEAAIKERGFELIQMDTEWTAKTGSPSKDGRSASEGEISEYTRESWEVLSMSTDDYRKATLFASGSFDQDFQIPDLVGQTEILSEEHREKLCAHLPARAEGYSWSLVFSTSQHGFSLNSLYRKMHKLESPILIVIEDTDHNVFGALTSCSLHVSDHFYGTGESLLYKFNPHFKVFHWSGENLYFIKGNPESLAIGAGDGKFGLWLDGDLNQGRSQHCSTYSNEPLAPQEDFVIKTLECWAFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V4B2</accession>
    <name>A0A6E8V4B2_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="16">G_PROTEIN_RECEP_F1_2 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8V4B2"/>
    <dbReference type="EnsemblMetazoa" id="ACON001773-RA">
        <property type="protein sequence ID" value="ACON001773-PA"/>
        <property type="gene ID" value="ACON001773"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004751"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001773"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039090"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15096">
        <property type="entry name" value="7tmA_AstA_R_insect"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000255">
        <property type="entry name" value="Allatostatin A receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000405">
        <property type="entry name" value="Galanin_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45695:SF23">
        <property type="entry name" value="GALANIN-LIKE G-PROTEIN COUPLED RECEPTOR NPR-9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45695">
        <property type="entry name" value="LEUCOKININ RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00663">
        <property type="entry name" value="GALANINR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 12 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="38"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="119"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="158"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="199"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="243"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="285"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="58"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="323"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001773-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="348" mass="38629" checksum="43C1F56900798B67" modified="2020-08-12" version="1">MVSENYTLMLMDATNRYNLSAFSTDAGPTMSHEDETEMIVSTIVAIFFGFIGITGLLGNALVVLVVLSNPQMRSTTNLLIINLAIADLLFVVFCIPFTATDYVLASWPFGELWCKSVQYLIVVTAHASIYTLVLMSLDRFLAVVHPIASMVIRTERNTVLAITILWVLIITTGLPVSFAHGINTCGFLEKDTPSAAFHIAFFLSSYVVPLILISVLYMWMLSRLWRSSVGGRSAESKKGKKRVTRLAVIVVAAFASLWFPIQVILVLKSVDVFKPDTHFKISLQIVSHVLAYTSSCINPLLYAFLSENFRKAFRKIVYCGPGSRRQGSQRHMPLPTKTTRTGSCPDIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V4C5</accession>
    <name>A0A6E8V4C5_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Tropomyosin</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the tropomyosin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8V4C5"/>
    <dbReference type="EnsemblMetazoa" id="ACON001797-RJ">
        <property type="protein sequence ID" value="ACON001797-PJ"/>
        <property type="gene ID" value="ACON001797"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002131"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001797"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039487"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="Tropomyosin alpha-1 chain"/>
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    </dbReference>
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        <property type="entry name" value="Tropomyosin alpha-1 chain isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000533">
        <property type="entry name" value="Tropomyosin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19269">
        <property type="entry name" value="TROPOMYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00261">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TROPOMYOSIN"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TROPOMYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="13"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001797-PJ"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="254" mass="29326" checksum="3FCD227540688056" modified="2020-08-12" version="1">MAANVQQQSGNLLDVLKKKMRQTKEEMEKYKDECEEFHKKLQNEVMRREEAESEVAALNRRIQLLEEDLERSEERLASATAKLSEASAAADESERIRKALENRTNMEDDRVGILEAQLAQAKLIAEEADKKYEEVARKLVLMEQDLERSEEKVEMNESKIVELEEELRVVGNNLKSLEVSEEKATQREESYGGQVRVLDQRLKEAEARAEFAERSVQKLQKEVDRLEDDLNKEKSKYKAIAAELDLTFADLSGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V8H8</accession>
    <name>A0A6E8V8H8_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Nucleoside phosphorylase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_049464679.1">
        <property type="nucleotide sequence ID" value="XM_049608722.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8V8H8"/>
    <dbReference type="EnsemblMetazoa" id="ACON000321-RA">
        <property type="protein sequence ID" value="ACON000321-PA"/>
        <property type="gene ID" value="ACON000321"/>
    </dbReference>
    <dbReference type="GeneID" id="120949910"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005282"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000321"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039442"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009116">
        <property type="term" value="P:nucleoside metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1580">
        <property type="entry name" value="Nucleoside phosphorylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055121">
        <property type="entry name" value="HTH_69"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035994">
        <property type="entry name" value="Nucleoside_phosphorylase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47705">
        <property type="entry name" value="AGAP000321-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47705:SF1">
        <property type="entry name" value="PNP_UDP_1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22979">
        <property type="entry name" value="HTH_69"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53167">
        <property type="entry name" value="Purine and uridine phosphorylases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Winged helix-turn-helix" evidence="2">
        <location>
            <begin position="210"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="507"/>
            <end position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000321-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="626" mass="69240" checksum="13328D0B04C4310D" modified="2023-06-28" version="2">MMSPTVKRLERPPPPPTPPKPERHPQQQQQHGADPGMPAVPSETPDEEQVVMTPLGKTNTSTVLLIERKLIQSVGERTHVAGGDHRGIIINKTAEEQSNRQREPAQLSLEFRVFLVSANSGQHSQESRRIKFWFRPWLTSENVQAQVAQDFFRELVSPKEFPRDYVGFIKKIMKLLQKGYNEIQAVEIELRILEQTSAPPSRPLSFEDEQFEVVPLTPEKILELIEQAYPNPIAPEDLAKDHGWEEEEVLAIMLVLKERGLIKSMEYNAFTRIHHDDQAIKIVKQMPTIASNKQPTIAIITAQYCEKLAVDAMLENKETFVRYTTVGESNVYTLGNIGAHRIVSTKLPSVGSTREAMTAAGNTTTRLLGTFQKVDYVFIVGVAGGIPHYTDYRRHVRLGDVVIAAAAHEPALTNGNGPKPYCYVYSGGQSEVKRYYPADDGLQAIGRTLVQNDDGKKPWLQYVQEGLQQLSGRGGHAEHDFARPAPNTDKLYMNIGNKNVIEVAHPIAQPSGEGDGGDDTDASGGGPVPHQTRLHAGPIGSGYELVRSDSARTEYAQAYQLLATDSEMNSVLDSIVGNCRDSFILVKGIADYKDGTTSRKWQNYASLAAAAVMKTIVCAMDAPTNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V8I0</accession>
    <name>A0A6E8V8I0_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 24">Lysine--tRNA ligase</fullName>
            <ecNumber evidence="6 24">6.1.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21 24">Lysyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="27 28">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="27">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="23">Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages. Catalyzes the synthesis of the signaling molecule diadenosine tetraphosphate (Ap4A), and thereby mediates disruption of the complex between HINT1 and MITF and the concomitant activation of MITF transcriptional activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22 24">
            <text>tRNA(Lys) + L-lysine + ATP = L-lysyl-tRNA(Lys) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:20792"/>
            <dbReference type="Rhea" id="RHEA-COMP:9696"/>
            <dbReference type="Rhea" id="RHEA-COMP:9697"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78529"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.6" evidence="6 24"/>
    <dbReference type="RefSeq" id="XP_040229555.2">
        <property type="nucleotide sequence ID" value="XM_040373621.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8V8I0"/>
    <dbReference type="EnsemblMetazoa" id="ACON000325-RA">
        <property type="protein sequence ID" value="ACON000325-PA"/>
        <property type="gene ID" value="ACON000325"/>
    </dbReference>
    <dbReference type="GeneID" id="120953554"/>
    <dbReference type="CTD" id="31904"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011325"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000325"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032707"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017101">
        <property type="term" value="C:aminoacyl-tRNA synthetase multienzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004824">
        <property type="term" value="F:lysine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006430">
        <property type="term" value="P:lysyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00775">
        <property type="entry name" value="LysRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04322">
        <property type="entry name" value="LysRS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000050">
        <property type="entry name" value="Lysine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000029">
        <property type="entry name" value="Lysine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00252">
        <property type="entry name" value="Lys_tRNA_synth_class2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002313">
        <property type="entry name" value="Lys-tRNA-ligase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034762">
        <property type="entry name" value="Lys-tRNA-ligase_II_bac/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044136">
        <property type="entry name" value="Lys-tRNA-ligase_II_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018149">
        <property type="entry name" value="Lys-tRNA-synth_II_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00499">
        <property type="entry name" value="lysS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001756">
        <property type="entry name" value="PRK00484.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42918:SF9">
        <property type="entry name" value="LYSINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42918">
        <property type="entry name" value="LYSYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039101">
        <property type="entry name" value="LysRS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00982">
        <property type="entry name" value="TRNASYNTHLYS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="17" id="KW-0007">Acetylation</keyword>
    <keyword evidence="19" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0436">Ligase</keyword>
    <keyword evidence="18" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0964">Secreted</keyword>
    <keyword evidence="13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="26">
        <location>
            <begin position="238"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="561"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="25">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="25">
        <location>
            <begin position="44"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00015745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00030563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00048573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00059215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="RuleBase" id="RU003748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000325-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="586" mass="65980" checksum="B4A8F4F5B4B6E46B" modified="2023-06-28" version="2">MADAKQGEEKLSKNELKRRLKAEAKEKEKQEKSAAAAQATDGGQVDKKQPAKENLDEISPNEYFKLRSAAVAELRKSPDTHPYPHKFSVTVSLGEFIERYSGLQDGETLDDVTVSVAGRVHAIRESGVKLIFFDLRGEGLKLQVMANAKAYESEQLFFEETARLRRGDIIGVTGVPGKTKKGELSAMARRIKLLSPCLHMLPHLHYGLKDKETRFRQRYLDLILNNNVRNIFVTRALIISYVRRFFDNLGFLEVETPMMNMIPGGATAKPFITHHNDLNMDLFLRIAPELYLKMLTVGGLDRVYEIGRQFRNEGIDLTHNPEFTTCEFYMAYADYNDIIDITQQLLSGMVHTIHGSYKVKYHPDGPEGDEYEIDFTPPFRRISMISSLEEALKVTFPAADQLHTPEAVQFLDALCVKHEVECKPPRTAARLLDKLVGEFLEETCINPTFICDHPQIMSPLAKYHRSATGLTERFELFVMRKEVCNAYTELNDPAVQRERFEQQAADKAAGDDEAQLVDENFCTALEYGLPPTGGWGMGIDRLTMFLTDSNNIKEVLLFPAMKPDDPNRKHPASVGAGGPEPGSSDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VA76</accession>
    <name>A0A6E8VA76_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="21">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Postsynaptic cell membrane</location>
            <topology evidence="16">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the ligand-gated ion channel (TC 1.A.9) family. Glutamate-gated chloride channel (TC 1.A.9.4) subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040240680.1">
        <property type="nucleotide sequence ID" value="XM_040384746.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VA76"/>
    <dbReference type="EnsemblMetazoa" id="ACON001434-RB">
        <property type="protein sequence ID" value="ACON001434-PB"/>
        <property type="gene ID" value="ACON001434"/>
    </dbReference>
    <dbReference type="GeneID" id="120961090"/>
    <dbReference type="CTD" id="42350"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_014247"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001434"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041837"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008068">
        <property type="term" value="F:extracellularly glutamate-gated chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18993">
        <property type="entry name" value="LGIC_ECD_GluCl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19062">
        <property type="entry name" value="LGIC_TM_GluCl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000024">
        <property type="entry name" value="Glutamate-gated chloride channel alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000022">
        <property type="entry name" value="glutamate-gated chloride channel isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006028">
        <property type="entry name" value="GABAA/Glycine_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044721">
        <property type="entry name" value="GluCl_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00860">
        <property type="entry name" value="LIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00253">
        <property type="entry name" value="GABAARECEPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0868">Chloride</keyword>
    <keyword evidence="11" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15 18" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 18" id="KW-0406">Ion transport</keyword>
    <keyword evidence="14" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="8 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0770">Synapse</keyword>
    <keyword evidence="3 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 18" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="244"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="309"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="431"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="19">
        <location>
            <begin position="34"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="20">
        <location>
            <begin position="251"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00061654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001434-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="456" mass="52417" checksum="22EB1F3A528591D8" modified="2020-08-12" version="1">MASGHFFWAIFYFACLCSASLANNAKVNFREKEKKILDQILGAGKYDARIRPSGINGTDGPAIVRINLFVRSIMTISDIKMEYSVQLTFREQWLDERLKFDDIGGRLKYLTLTEANRVWMPDLFFSNEKEGHFHNIIMPNVYIRIFPYGSVLYSIRISLTLACPMNLKLYPLDRQVCSLRMASYGWTTADLVFLWKEGDPVQVVKNLHLPRFTLEKFLTDYCNSKTNTGEYSCLKVDLLFKREFSYYLIQIYIPCCMLVIVSWVSFWLDQGAVPARVSLGVTTLLTMATQTSGINASLPPVSYTKAIDVWTGVCLTFVFGALLEFALVNYASRSADRAADMHRENMKKKRREMEQASLDAASDLLDTDSNATFAMKPLVRHPGDPLALEKLRQCEVHMQAPKRPNCCRSWLSKFPTRQCSRSKRIDVISRITFPLVFALFNLVYWSTYLFREEEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VA84</accession>
    <name>A0A6E8VA84_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">CBS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="4">AMPK is a heterotrimer of an alpha catalytic subunit (PRKAA1 or PRKAA2), a beta (PRKAB1 or PRKAB2) and a gamma non-catalytic subunits (PRKAG1, PRKAG2 or PRKAG3). Interacts with FNIP1 and FNIP2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the 5'-AMP-activated protein kinase gamma subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VA84"/>
    <dbReference type="EnsemblMetazoa" id="ACON001446-RC">
        <property type="protein sequence ID" value="ACON001446-PC"/>
        <property type="gene ID" value="ACON001446"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002448"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001446"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040820"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031588">
        <property type="term" value="C:nucleotide-activated protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019887">
        <property type="term" value="F:protein kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04618">
        <property type="entry name" value="CBS_euAMPK_gamma-like_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04641">
        <property type="entry name" value="CBS_euAMPK_gamma-like_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050511">
        <property type="entry name" value="AMPK_gamma/SDS23_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780">
        <property type="entry name" value="AMP-ACTIVATED PROTEIN KINASE, GAMMA REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780:SF35">
        <property type="entry name" value="LD22662P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0129">CBS domain</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CBS" evidence="7">
        <location>
            <begin position="303"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="7">
        <location>
            <begin position="384"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="7">
        <location>
            <begin position="457"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="7">
        <location>
            <begin position="531"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="590"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="77"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="137"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="594"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="606"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="629"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="674"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="703"/>
            <end position="717"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001446-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="741" mass="82081" checksum="4A89FDEC9D256C4A" modified="2020-08-12" version="1">MKQKREEARRLEKEAAKLEKLNRKHESLSRSSERVGSGARSGSLERRRSGEEAPVLNQSTVHGIASPNRRPTIFDVFRPRKGSDSKKKKDDGSKSGSDKDSTGMGSGPISTSGGIMNSMKAALHVGGRHSHQHQQPAGATGAAATAGTSSKVRDGSAHPHAGSDAQYYHTVTAVRRADVGKSPMTKVMDLFRHRSNSAVSEADKRKAKAAAQHQQQLAVQSAHMRRASAELAERRRASLGATRGLRADGTLDPYHAAILFRDSRGLPVADPFLEKVPLSDLEEDESQIFVKFFRFHKCYDLVPTSAKLVVFDTQLLVKKAFYALVYNGVRAAPLWDSKRQEFVGMLTITDFIKILKMYYKSPNASMDELEEHKLDTWRKVLQEDVKKLVSIGPDASLYDAIKMLVHNRIHRLPVIDPVTGNVLYILTHKRILRFLFLYINELPKPSYMQKTLREVRIGSYNNIETATEDTSIITALHKFVDRRVSALPMVDSEGRLTDIYAKFDVINLAAEKTYNDLDVSLKKANEHRNAWFEGVHHCTLDETLYTIMERIVRVEVHRLVVVDELKKVIGIISLSDILLYLVLRPSGDGIGDSESLRATDPRLMKAGSPAGGSSSSKLQATKRDSSNESIEEEKETTEEDAEQEQQDAAAHKTETTITPATPSNDEDGQKNEAEVEVETADDAVPELAVEELAEEAKEDPDDTASTPVDRSSANDSPVAQEMLATDVPLQSVQREVGLVSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VA85</accession>
    <name>A0A6E8VA85_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cyclin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the cyclin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VA85"/>
    <dbReference type="EnsemblMetazoa" id="ACON001677-RA">
        <property type="protein sequence ID" value="ACON001677-PA"/>
        <property type="gene ID" value="ACON001677"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005318"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001677"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_029587"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043198">
        <property type="entry name" value="Cyclin/Ssn8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10026">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0195">Cyclin</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="4">
        <location>
            <begin position="83"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="189"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001677-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="216" mass="24077" checksum="C57B50A21486FC27" modified="2020-08-12" version="1">MSVSKTITETVSATGKAASAVIGTTQSTPATAAAPQPVQRPYGKIVLTLENCLLPEVKLDQTPSQNDGLDRETETDLRILGCELIQTAGILLKLPQVAMATGQVLFQRFFYSKSFVRHSMEATAMSCICLASKIEEAPRRIRDVINVFHHIKQVRSQNFVGKTQSYSKLYLLLKATLSAQSFRVQKKQTKQNKKIQIKHTKTKHGLNRTKQNKIHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VCA7</accession>
    <name>A0A6E8VCA7_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Sodium/potassium-transporting ATPase subunit alpha</fullName>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="22">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.</text>
    </comment>
    <comment type="subunit">
        <text evidence="19">The sodium/potassium-transporting ATPase is composed of a catalytic alpha subunit, an auxiliary non-catalytic beta subunit and an additional regulatory subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 20">Cell membrane</location>
            <topology evidence="1 20">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 20">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VCA7"/>
    <dbReference type="EnsemblMetazoa" id="ACON002858-RC">
        <property type="protein sequence ID" value="ACON002858-PC"/>
        <property type="gene ID" value="ACON002858"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006768"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002858"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039874"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005391">
        <property type="term" value="F:P-type sodium:potassium-exchanging transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030007">
        <property type="term" value="P:intracellular potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006883">
        <property type="term" value="P:intracellular sodium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036376">
        <property type="term" value="P:sodium ion export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02608">
        <property type="entry name" value="P-type_ATPase_Na-K_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000003">
        <property type="entry name" value="Sodium/potassium-transporting ATPase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000001">
        <property type="entry name" value="Sodium/potassium-transporting ATPase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000004">
        <property type="entry name" value="Sodium/potassium-transporting ATPase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000095">
        <property type="entry name" value="Sodium/potassium-transporting ATPase subunit alpha-1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050510">
        <property type="entry name" value="Cation_transp_ATPase_P-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005775">
        <property type="entry name" value="P-type_ATPase_IIC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01106">
        <property type="entry name" value="ATPase-IIC_X-K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43294">
        <property type="entry name" value="SODIUM/POTASSIUM-TRANSPORTING ATPASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43294:SF13">
        <property type="entry name" value="SODIUM_POTASSIUM-TRANSPORTING ATPASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00121">
        <property type="entry name" value="NAKATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15 20" id="KW-0406">Ion transport</keyword>
    <keyword evidence="16 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11 20" id="KW-0630">Potassium</keyword>
    <keyword evidence="5 20" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0915">Sodium</keyword>
    <keyword evidence="17" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="7" id="KW-0740">Sodium/potassium transport</keyword>
    <keyword evidence="12" id="KW-1278">Translocase</keyword>
    <keyword evidence="8 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 20" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="73"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="107"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="268"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="298"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="830"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="891"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="929"/>
            <end position="950"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="962"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="21">
        <location>
            <begin position="19"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00037422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00038795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU362084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002858-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1000" mass="110870" checksum="7A0E07806C8C0891" modified="2020-08-12" version="1">MPPKKKTDLDDLKQELDIDYHKITPEELYQRLQTHPENGLSHAKAKENLERDGPNALTPPKQTPEWVKFCKNLFGGFALLLWIGAILCFIAYSIQASTVEEPADDNLYLGIVLAAVVIVTGIFSYYQESKSSKIMESFKNMVPQFATVLREGEKLTLRAEDLVIGDVVEVKFGDRIPADIRIIEARNFKVDNSSLTGESEPQSRGPDFTHENPLETKNLAFFSTNAVEGTAKGVVISCGDFTVMGRIAGLASGLDTGETPIAKEIHHFIHLITGVAVFLGVTFFVIAFILGYHWLDAVIFLIGIIVANVPEGLLATVTVCLTLTAKRMASKNCLVKNLEAVETLGSTSTICSDKTGTLTQNRMTVAHMWFDNQIIEADTTEDQSGVQYDRTSPGFKALSRIASLCNRAEFKGGQDGVPILKKEVSGDASEAALLKCMELALGDVLSIRKRNKKVCEIPFNSTNKYQVSIHETEDPSDPRYLLVMKGAPERILERCSTIFINGKEKLMDEEMKEAFNNAYLELGGLGERVLGFCDFMLPSDKYPVGFKFNSDDVNFQVDNLRFVGLMSMIDPPRAAVPDAVAKCRSAGIKVIMVTGDHPITAKAIAKSVGIISEGNETVEDIAQRLNIPVSEVNPREAKAAVVHGSELRDLSSDQLDEILRYHTEIVFARTSPQQKLIIVEGCQRMGAIVAVTGDGVNDSPALKKADIGVAMGIAGSDVSKQAADMILLDDNFASIVTGVEEGRLIFDNLKKSIAYTLTSNIPEISPFLAFILCDIPLPLGTVTILCIDLGTDMVPAISLAYEEAESDIMKRQPRNPFTDKLVNERLISMAYGQIGMIQAAAGFFVYFVIMAENGFLPLKLFGLRKSWDSKAINDLTDSYGQEWTYRDRKTLEFTCHTAFFVSIVVVQWADLIICKTRRNSIFHQGMRNWALNFGLVFETVLAAILSYTPGMDKGLRMYPLKFVWWLPALPFSLSIFVYDEIRRFYLRRNPGGWLEQETYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VEB8</accession>
    <name>A0A6E8VEB8_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="7">CLIC N-terminal domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the chloride channel CLIC family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_049463631.1">
        <property type="nucleotide sequence ID" value="XM_049607674.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VEB8"/>
    <dbReference type="EnsemblMetazoa" id="ACON000943-RA">
        <property type="protein sequence ID" value="ACON000943-PA"/>
        <property type="gene ID" value="ACON000943"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON000943-RB">
        <property type="protein sequence ID" value="ACON000943-PB"/>
        <property type="gene ID" value="ACON000943"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON000943-RC">
        <property type="protein sequence ID" value="ACON000943-PC"/>
        <property type="gene ID" value="ACON000943"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON000943-RD">
        <property type="protein sequence ID" value="ACON000943-PD"/>
        <property type="gene ID" value="ACON000943"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON000943-RF">
        <property type="protein sequence ID" value="ACON000943-PF"/>
        <property type="gene ID" value="ACON000943"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON000943-RG">
        <property type="protein sequence ID" value="ACON000943-PG"/>
        <property type="gene ID" value="ACON000943"/>
    </dbReference>
    <dbReference type="GeneID" id="120961279"/>
    <dbReference type="CTD" id="32349"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_008503"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000943"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033232"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03198">
        <property type="entry name" value="GST_C_CLIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03061">
        <property type="entry name" value="GST_N_CLIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000040">
        <property type="entry name" value="chloride intracellular channel exc-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000188">
        <property type="entry name" value="Chloride intracellular channel exc-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053823">
        <property type="entry name" value="CLIC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43920:SF5">
        <property type="entry name" value="CHLORIDE INTRACELLULAR CHANNEL CLIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43920">
        <property type="entry name" value="CHLORIDE INTRACELLULAR CHANNEL, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22441">
        <property type="entry name" value="CLIC-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="CLIC N-terminal" evidence="6">
        <location>
            <begin position="17"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF22441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000943-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="257" mass="29587" checksum="A6AC0528D0B2A009" modified="2020-08-12" version="1">MSDEGQDNGASRNGSVPEIELIIKASTIDGRRKGACLFCQEYFMDLYLLAELKTISLKVTTVCMQKPPPDFRTNFEATHPPILIDNGLAILENEKIERHIMKSVPGGYNLFVQDKEVATLIENLYSKLKLMLVKKDENKNNALLVHLQKINDHLAARGTRFLTGDTMCCFDCELMPRLQHIRVAGKYFVDFDIPKHLTALWRYMYHMYQLDAFTQSCPADQDIINHYKLQQMLKMKKHEELETPTFTTSIPVDLNEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VEC2</accession>
    <name>A0A6E8VEC2_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Pribosyltran_N domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ribose-phosphate pyrophosphokinase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040233894.1">
        <property type="nucleotide sequence ID" value="XM_040377960.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040233979.1">
        <property type="nucleotide sequence ID" value="XM_040378045.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040234067.1">
        <property type="nucleotide sequence ID" value="XM_040378133.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040234159.1">
        <property type="nucleotide sequence ID" value="XM_040378225.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040234328.1">
        <property type="nucleotide sequence ID" value="XM_040378394.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040234412.1">
        <property type="nucleotide sequence ID" value="XM_040378478.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_049466135.1">
        <property type="nucleotide sequence ID" value="XM_049610178.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VEC2"/>
    <dbReference type="EnsemblMetazoa" id="ACON000954-RD">
        <property type="protein sequence ID" value="ACON000954-PD"/>
        <property type="gene ID" value="ACON000954"/>
    </dbReference>
    <dbReference type="GeneID" id="120956298"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000223"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000954"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_029756"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002189">
        <property type="term" value="C:ribose phosphate diphosphokinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004749">
        <property type="term" value="F:ribose phosphate diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006015">
        <property type="term" value="P:5-phosphoribose 1-diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000024">
        <property type="entry name" value="Putative phosphoribosyl pyrophosphate synthase-associated protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000036">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029099">
        <property type="entry name" value="Pribosyltran_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005946">
        <property type="entry name" value="Rib-P_diPkinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01251">
        <property type="entry name" value="ribP_PPkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210:SF53">
        <property type="entry name" value="GH23275P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210">
        <property type="entry name" value="RIBOSE-PHOSPHATE DIPHOSPHOKINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14572">
        <property type="entry name" value="Pribosyl_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13793">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01400">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribose-phosphate pyrophosphokinase N-terminal" evidence="4">
        <location>
            <begin position="9"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="201"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000954-PD"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="355" mass="38949" checksum="4AB9B105801822A0" modified="2020-08-12" version="1">MDAPATSDIVIINGNSHPDLANLIANRLGVANGGCAVYHKTNRETMVEIGDSVRGKDIYIIQTGTKDVNNNIMELLIMAYACKTSSAKSIVGVIPYLPYSKQCKMRKRGCIVSKLLAKMMCTSGLSHIITMDLHQKEIQGFFDCPVDNLRASPFLLQYIQESIPDYRNSVIVARNPGSAKKATSYAERLRLGIAVIHGEQKESEADEVDGRNSPPTLPKSRTMDVSGGVPIHPAKEKPPINVVGDVGGRIAIMVDDLIDDVQSFVAAAEVLKDRGAYKIYVLATHGLLSQDAPRLIEDSAIDVVVVTNTIPHEIQKMQCHKIKTIDISILLAEAIRRIHNKESMSYLFKNVTLED</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VEE3</accession>
    <name>A0A6E8VEE3_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Troponin I</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the troponin I family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_049463039.1">
        <property type="nucleotide sequence ID" value="XM_049607082.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_049463053.1">
        <property type="nucleotide sequence ID" value="XM_049607096.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VEE3"/>
    <dbReference type="EnsemblMetazoa" id="ACON001053-RA">
        <property type="protein sequence ID" value="ACON001053-PA"/>
        <property type="gene ID" value="ACON001053"/>
    </dbReference>
    <dbReference type="GeneID" id="120948222"/>
    <dbReference type="CTD" id="32794"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007438"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001053"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039909"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005861">
        <property type="term" value="C:troponin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006936">
        <property type="term" value="P:muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.350:FF:000004">
        <property type="entry name" value="WupA, isoform I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001978">
        <property type="entry name" value="Troponin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050875">
        <property type="entry name" value="Troponin_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038077">
        <property type="entry name" value="Troponin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13738">
        <property type="entry name" value="TROPONIN I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13738:SF1">
        <property type="entry name" value="TROPONIN I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00992">
        <property type="entry name" value="Troponin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90250">
        <property type="entry name" value="Troponin coil-coiled subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="2" id="KW-0488">Methylation</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00074692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001053-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="197" mass="23673" checksum="B5B0060D9799CABF" modified="2020-08-12" version="1">MADDEAKKAKQAEIERKRAEVRKRMEEASKAKKAKKGFMTPERKKKLRLLLRKKAAEELKKEQERKAAERRRIIEERCGKPKNVEDANEDQLKDIVKEYYNRMYTCEGQKWDLEYEVRKRDWEISDLNAQVNDLRGKFVKPTLKKVSKYENKFAKLQKKAAEFNFRNQLKVVKKKEFTLEEEDKEAKKSEKADWQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VEE4</accession>
    <name>A0A6E8VEE4_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Troponin I</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the troponin I family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040220275.1">
        <property type="nucleotide sequence ID" value="XM_040364341.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040220300.1">
        <property type="nucleotide sequence ID" value="XM_040364366.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VEE4"/>
    <dbReference type="EnsemblMetazoa" id="ACON001053-RF">
        <property type="protein sequence ID" value="ACON001053-PF"/>
        <property type="gene ID" value="ACON001053"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON001053-RH">
        <property type="protein sequence ID" value="ACON001053-PH"/>
        <property type="gene ID" value="ACON001053"/>
    </dbReference>
    <dbReference type="GeneID" id="120948222"/>
    <dbReference type="KEGG" id="acoz:120948222"/>
    <dbReference type="CTD" id="32794"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007438"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001053"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039909"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005861">
        <property type="term" value="C:troponin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006936">
        <property type="term" value="P:muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.350:FF:000004">
        <property type="entry name" value="WupA, isoform I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001978">
        <property type="entry name" value="Troponin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050875">
        <property type="entry name" value="Troponin_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038077">
        <property type="entry name" value="Troponin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13738">
        <property type="entry name" value="TROPONIN I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13738:SF1">
        <property type="entry name" value="TROPONIN I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00992">
        <property type="entry name" value="Troponin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90250">
        <property type="entry name" value="Troponin coil-coiled subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="2" id="KW-0488">Methylation</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="181"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="181"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00074692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001053-PF"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="210" mass="24958" checksum="25EE937A05AB0621" modified="2020-08-12" version="1">MADDEAKKAKQAEIERKRAEVRKRMEEASKAKKAKKGFMTPERKKKLRLLLRKKAAEELKKEQERKAAERRRIIEERCGKPKNVEDANEDQLKDIVKEYYNRMYTCEGQKWDLEYEVRKRDWEISDLNAQVNDLRGKFVKPTLKKVSKYENKFAKLQKKAAEFNFRNQLKVVKKKEFTLEEEDKEKKPDWSKKGDSKVKEEAAEAVEAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VFZ1</accession>
    <name>A0A6E8VFZ1_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="9">PHB domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the band 7/mec-2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040221261.1">
        <property type="nucleotide sequence ID" value="XM_040365327.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VFZ1"/>
    <dbReference type="EnsemblMetazoa" id="ACON003352-RB">
        <property type="protein sequence ID" value="ACON003352-PB"/>
        <property type="gene ID" value="ACON003352"/>
    </dbReference>
    <dbReference type="GeneID" id="120948701"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000005"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003352"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030318"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13435">
        <property type="entry name" value="SPFH_SLP-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.479.30:FF:000002">
        <property type="entry name" value="band 7 protein AGAP004871"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2090">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="entry name" value="Band 7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043202">
        <property type="entry name" value="Band-7_stomatin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001972">
        <property type="entry name" value="Stomatin_HflK_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10264:SF19">
        <property type="entry name" value="AT06885P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10264">
        <property type="entry name" value="BAND 7 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00721">
        <property type="entry name" value="STOMATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="Band 7/SPFH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="44"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="Band 7" evidence="8">
        <location>
            <begin position="64"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="305"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="317"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003352-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="337" mass="37050" checksum="BEFAD8E5FFB3A891" modified="2020-08-12" version="1">MYHAQHLQTQQLQPPNQQHTSQQQQDQVRWKVKTEADSIGCVEVLATVCSIVLMVLTLPISLFLCFKVVQEYERAVIFRLGRLRSGGARGPGVFFVLPCIDNYCKVDLRTVSFDVPPQEVLTRDSVTVSVDAVVYYRIRDPLNAVVQVANYSHSTRLLAATTLRNVLGTRNLSELLTEREAISHSMQVTLDEATDPWGVQVERVEIKDVSLPDSLQRSMAAEAEAAREARAKVIAAEGEMKSSRALKEASDIMCESPAALQLRYLQTLSSIAGEKNSTIVFPLPIELIGPLMNFTSSLGVQRTTAPVRPMAPPAAPPSTTTSTSSPLGDPSQQQSFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VGF1</accession>
    <name>A0A6E8VGF1_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Inorganic pyrophosphatase</fullName>
            <ecNumber evidence="4">3.6.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Pyrophosphate phospho-hydrolase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">Ngousso</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.1" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8VGF1"/>
    <dbReference type="EnsemblMetazoa" id="ACON003398-RA">
        <property type="protein sequence ID" value="ACON003398-PA"/>
        <property type="gene ID" value="ACON003398"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON003398-RB">
        <property type="protein sequence ID" value="ACON003398-PB"/>
        <property type="gene ID" value="ACON003398"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001536"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003398"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030944"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004427">
        <property type="term" value="F:inorganic diphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006796">
        <property type="term" value="P:phosphate-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00412">
        <property type="entry name" value="pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.80.10:FF:000004">
        <property type="entry name" value="Inorganic pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.80.10">
        <property type="entry name" value="Inorganic pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008162">
        <property type="entry name" value="Pyrophosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036649">
        <property type="entry name" value="Pyrophosphatase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10286">
        <property type="entry name" value="INORGANIC PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00719">
        <property type="entry name" value="Pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50324">
        <property type="entry name" value="Inorganic pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00387">
        <property type="entry name" value="PPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003398-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="380" mass="41996" checksum="7F4DEE813E55B3B7" modified="2020-08-12" version="1">MYPAVCWWRRIAWRGACALPSGTFPDAIRPRSHQPSFPPNSTSTRCHSSFFAATDVQRFCGGHLSSVGDSKLLPSASLSRVGQLSGREFSSSNKAKHKMTASKYQIAERGAPNSTDYRVYFKNENGQSISPLHDIPLYANDERTVYNMVVEVPRWTNAKMEISLGEGLNPIKQDVKKGKLRFVANCFPHHGYIWNYGAFPQTWENPDHLDADTGCKGDNDPIDVLEIGSRIAKRGEVLQVKILGTIALIDEGETDWKIITINVNDPLVDQVNDIGDVETVFPGLLRASVEWFKIYKIPDGKPENQFAFNGDAKDAAFATKTVAETHRFWQQLVNKEVDNNGISCLNTTVDGSPYLVANDAADEVFAKSATGGNPEPVSEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VGF3</accession>
    <name>A0A6E8VGF3_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phenylalanine--tRNA ligase beta subunit</fullName>
            <ecNumber evidence="5">6.1.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Phenylalanyl-tRNA synthetase beta subunit</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>tRNA(Phe) + L-phenylalanine + ATP = L-phenylalanyl-tRNA(Phe) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19413"/>
            <dbReference type="Rhea" id="RHEA-COMP:9668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78531"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.20" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8VGF3"/>
    <dbReference type="EnsemblMetazoa" id="ACON003517-RA">
        <property type="protein sequence ID" value="ACON003517-PA"/>
        <property type="gene ID" value="ACON003517"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002957"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003517"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040865"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009328">
        <property type="term" value="C:phenylalanine-tRNA ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004826">
        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006432">
        <property type="term" value="P:phenylalanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00769">
        <property type="entry name" value="PheRS_beta_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000032">
        <property type="entry name" value="Phenylalanine--tRNA ligase beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.40.10:FF:000002">
        <property type="entry name" value="phenylalanine--tRNA ligase beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.56.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.40.10">
        <property type="entry name" value="Phenylalanyl-trna Synthetase, Chain B, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005146">
        <property type="entry name" value="B3/B4_tRNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045060">
        <property type="entry name" value="Phe-tRNA-ligase_IIc_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004531">
        <property type="entry name" value="Phe-tRNA-synth_IIc_bsu_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020825">
        <property type="entry name" value="Phe-tRNA_synthase-like_B3/B4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041616">
        <property type="entry name" value="PheRS_beta_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040659">
        <property type="entry name" value="PhetRS_B1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005147">
        <property type="entry name" value="tRNA_synthase_B5-dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00471">
        <property type="entry name" value="pheT_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10947:SF0">
        <property type="entry name" value="PHENYLALANINE--TRNA LIGASE BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10947">
        <property type="entry name" value="PHENYLALANYL-TRNA SYNTHETASE BETA CHAIN AND LEUCINE-RICH REPEAT-CONTAINING PROTEIN 47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03483">
        <property type="entry name" value="B3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03484">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18262">
        <property type="entry name" value="PhetRS_B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17759">
        <property type="entry name" value="tRNA_synthFbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00873">
        <property type="entry name" value="B3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00874">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56037">
        <property type="entry name" value="PheT/TilS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="Putative DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51483">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0436">Ligase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B5" evidence="17">
        <location>
            <begin position="286"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00017032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00033189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003517-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="570" mass="63794" checksum="55643EDC2CDA2275" modified="2020-08-12" version="1">DDEFQKFCFEFGLELDEVTTEKQMITKEQGLVEAAKDASEEIIYRIDIPANRYDLLCLEGLVMGLQVFMGKIKFPRFTKVGPVGKGAAPQKLIVTKATAQIRPFAVAAVLRDITFTKDSYDSFIDLQDKLHQNICRKRTLVAIGTHDLDKLQGPFTFDAKPPKDIRFVPLNQEKAMTGEELMEFYSTHAQLKTYLPIIRDSPVYPVIYDANGVVLSLPPIINGDHSKITLQTKNVFIECTATDLTKARIVLDTLVCMFSTHCAKQFTVEYCEVVSASGETKQYPDLAFRKETISVAETNAYIGLNESAEKMAYLLNRLLPTKQTAPDTLEVEVPPTRHDILHACDIYEDVAIGYGYNRIPKTLPAKMHIAKQYPLNKLTEQLREQIAQAGFTEGLTFTLCSRDDIATKMNAQIDQIPAVHIANPKTLEFQVVRTTLIPGLLKTLAANRKMPLPLKLFEVSDVVLADGKSEVGAKNERRVCAINCNKTAGFEVVHGLLDRVMQLLEVPWDRQTGYYLQACDDPAYFPGRCASVLYKGAAIGRIGVLHPTVLQAFELTTPCSVVEFNMEYFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VGF4</accession>
    <name>A0A6E8VGF4_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein Skeletor</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_040224137.2">
        <property type="nucleotide sequence ID" value="XM_040368203.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VGF4"/>
    <dbReference type="EnsemblMetazoa" id="ACON003513-RB">
        <property type="protein sequence ID" value="ACON003513-PB"/>
        <property type="gene ID" value="ACON003513"/>
    </dbReference>
    <dbReference type="GeneID" id="120950289"/>
    <dbReference type="CTD" id="131892018"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002984"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003513"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038364"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019545">
        <property type="entry name" value="DM13_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052126">
        <property type="entry name" value="Spindle_Org/Thrombomodulin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24036:SF13">
        <property type="entry name" value="PROTEIN SKELETOR, ISOFORMS D_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24036">
        <property type="entry name" value="SKELETOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10517">
        <property type="entry name" value="DM13"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00686">
        <property type="entry name" value="DM13"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51549">
        <property type="entry name" value="DM13"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="DM13" evidence="2">
        <location>
            <begin position="42"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="DM13" evidence="2">
        <location>
            <begin position="158"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003513-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="287" mass="31779" checksum="D9D85B6E124360F3" modified="2020-08-12" version="1">MLLTSGSTSSVRQRSRVIVSVASMILICFLADQTSALQGYYGTKIGDLSELHHGVSGSVYAVDARTLFLKNFNYDGEGPAAYFYVGNTRAPSNKGAHRLRDERGRAGVLRRYRNEDITLSLPEGKTLRDIRWFSVWCDDFSVNFGDVQIRNDLDFPRPTKIAGLSGVHDVSSDNIVIVDAQTLLVPNFSYDGEAPDAKFWVGRGPAPTSQGIRIPDENGKETPLRRYDKKTIVLTLPGDLTVFDIGHFGVWCEAFTVDFGHVRIPDQINVPPSLKMLGISPQRSRSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VGG0</accession>
    <name>A0A6E8VGG0_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Inorganic pyrophosphatase</fullName>
            <ecNumber evidence="4">3.6.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Pyrophosphate phospho-hydrolase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">Ngousso</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.1" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8VGG0"/>
    <dbReference type="EnsemblMetazoa" id="ACON003398-RF">
        <property type="protein sequence ID" value="ACON003398-PF"/>
        <property type="gene ID" value="ACON003398"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001536"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003398"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030944"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004427">
        <property type="term" value="F:inorganic diphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006796">
        <property type="term" value="P:phosphate-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00412">
        <property type="entry name" value="pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.80.10:FF:000004">
        <property type="entry name" value="Inorganic pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.80.10">
        <property type="entry name" value="Inorganic pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008162">
        <property type="entry name" value="Pyrophosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036649">
        <property type="entry name" value="Pyrophosphatase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10286">
        <property type="entry name" value="INORGANIC PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00719">
        <property type="entry name" value="Pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50324">
        <property type="entry name" value="Inorganic pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00387">
        <property type="entry name" value="PPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003398-PF"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="284" mass="31575" checksum="06E1520B8367068C" modified="2020-08-12" version="1">MTASKYQIAERGAPNSTDYRVYFKNENGQSISPLHDIPLYANDERTVYNMVVEVPRWTNAKMEISLGEGLNPIKQDVKKGKLRFVANCFPHHGYIWNYGAFPQTWENPDHLDADTGCKGDNDPIDVLEIGSRIAKRGEVLQVKILGTIALIDEGETDWKIITINVNDPLVDQVNDIGDVETVFPGLLRASVEWFKIYKIPDGKPENQFAFNGDAKDAAFATKTVAETHRFWQQLVNKEVDNNGISCLNTTVDGSPYLVANDAADEVFAKSATGGNPEPVSEARK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VGG8</accession>
    <name>A0A6E8VGG8_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="6">NOT2_3_5 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CNOT2/3/5 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VGG8"/>
    <dbReference type="EnsemblMetazoa" id="ACON003525-RB">
        <property type="protein sequence ID" value="ACON003525-PB"/>
        <property type="gene ID" value="ACON003525"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_013063"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003525"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031874"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030015">
        <property type="term" value="C:CCR4-NOT core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000036">
        <property type="term" value="P:regulation of stem cell population maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.1020:FF:000005">
        <property type="entry name" value="Regena, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.1020">
        <property type="entry name" value="CCR4-NOT complex subunit 2/3/5, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038635">
        <property type="entry name" value="CCR4-NOT_su2/3/5_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040168">
        <property type="entry name" value="Not2/3/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007282">
        <property type="entry name" value="NOT2/3/5_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23326">
        <property type="entry name" value="CCR4 NOT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04153">
        <property type="entry name" value="NOT2_3_5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NOT2/NOT3/NOT5 C-terminal" evidence="5">
        <location>
            <begin position="310"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="116"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="167"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="119"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="173"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="205"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="229"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003525-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="451" mass="47530" checksum="95E466B185AD4986" modified="2020-08-12" version="1">MCSVTLSQKGMPQKGYYHQRTASVSSGSSSLATIGVGSAAAAGSVAVGGVAGGGIVGGSGGSTVTATAAAVGNSNMVSFMQSRGYGSQSGNSINNYHGVFDGSAAETGTPPLLDLSEFPSLTNARGGQNDQSLPQSNALQPPGSKPYVGMVKQPTSEQIAFTMSNEDFPALPGTQNAEGSQMGAASQGLQGVHHHHHPDHHGGTDSLGGKGAGGSGQVGSAAGMGMDLQSDSGNSNVNMDKGAMKRGVQTSPNGTVTNIPASMVNNQFGMVGLLTFLRAAETDPNLVTLAMGQDLMALGLNLTAVENLYPCFGGPFADAPARPQDIEYHVPPEYLINMSIRDKLSKLTLQKYKDDLLFYLFYTNVGDMMQLAAAAELHSRDWRYHTEEKVWITRVPGMTPYEKNGTTERGTYYYFDAQTWRRVPKEFQVDTMKLDKCPNISAYVTMSGQPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VGH8</accession>
    <name>A0A6E8VGH8_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="7">BTB domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VGH8"/>
    <dbReference type="EnsemblMetazoa" id="ACON003537-RA">
        <property type="protein sequence ID" value="ACON003537-PA"/>
        <property type="gene ID" value="ACON003537"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_014181"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003537"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_029744"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045165">
        <property type="term" value="P:cell fate commitment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042051">
        <property type="term" value="P:compound eye photoreceptor development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002009">
        <property type="term" value="P:morphogenesis of an epithelium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061061">
        <property type="term" value="P:muscle structure development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048477">
        <property type="term" value="P:oogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045466">
        <property type="term" value="P:R7 cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18315">
        <property type="entry name" value="BTB_POZ_BAB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000091">
        <property type="entry name" value="Lola, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051095">
        <property type="entry name" value="Dros_DevTransReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110">
        <property type="entry name" value="BTB DOMAIN TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110:SF82">
        <property type="entry name" value="PROTEIN TRAMTRACK, ALPHA ISOFORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BTB" evidence="6">
        <location>
            <begin position="31"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="116"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="252"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="481"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="526"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="123"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="257"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="340"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="380"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="424"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="458"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="536"/>
            <end position="548"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003537-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="647" mass="69652" checksum="FECB3BC8E12830BD" modified="2020-08-12" version="1">MTSQRFCLRWNNHQTNLLAVFDQLLHDETFIDVTLAVEGQHLKAHKMVLSACSPYFQQLFVSHPEKHPIVILRDVPFKDMKCLLDFMYRGEVSVDQDRLAAFLRVAESLRIKGLTEVNDDKPNAPSGSASATATNSAGSAASSGAQATGATTTTNTVPNATVQKNSSGSSGMVAQVDDDEDDRCSPVPNSIAVAPNAASSGQAQGGHLKPLPPLLSSTGIVTRSHSNSLLQQGGSSAASGSAMVQTNPLLGTALSQQKRKRGRPRKLSGSDAGEGETEGNFVGGGGAPGEEYDSSSMDIMDVKIASSGASNSGDDDQQLRIVSSRTDDDDTLMHEKAEQLDDEDDEVMDDEADELVDDVDLEAGDEPRGTSPSLDESMEDTTGNMDGTTNNNGNTNRSGRQHQKQGTAAAKPSRASKRSRTRNNENSLSGSELSIFPINLEKKDHNTNAVATNPINGAEEEEESDEEIEFILPKAYCAKFSDEAASNPPESRDGDTLPPLLQELKRNGHLQDVTIIERRRSFDVPLVHQTANGTDDSPKEAKAPKLDETDNDEPSDLRVRATAQDTPTPEAEAITELENRTDHINMLSSAFSAKPMRIPATAVTKRRTRHTISPNDPAKALTEMSVRGLDLFQYATINGGMYQHGVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VIH9</accession>
    <name>A0A6E8VIH9_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glutathione peroxidase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the glutathione peroxidase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VIH9"/>
    <dbReference type="EnsemblMetazoa" id="ACOM043132-RB">
        <property type="protein sequence ID" value="ACOM043132-PB.1"/>
        <property type="gene ID" value="ACOM043132"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON004247-RB">
        <property type="protein sequence ID" value="ACON004247-PB"/>
        <property type="gene ID" value="ACON004247"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001342"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004247"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_043019"/>
    <dbReference type="Proteomes" id="UP000075882">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00340">
        <property type="entry name" value="GSH_Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000025">
        <property type="entry name" value="Glutathione peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000889">
        <property type="entry name" value="Glutathione_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11592">
        <property type="entry name" value="GLUTATHIONE PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11592:SF78">
        <property type="entry name" value="GLUTATHIONE PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00255">
        <property type="entry name" value="GSHPx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000303">
        <property type="entry name" value="Glutathion_perox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01011">
        <property type="entry name" value="GLUTPROXDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51355">
        <property type="entry name" value="GLUTATHIONE_PEROXID_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2 5" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044631333" description="Glutathione peroxidase" evidence="6">
        <location>
            <begin position="23"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="7">
        <location>
            <begin position="39"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000303-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004247-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="202" mass="22235" checksum="B2896DF5D5B13B0A" modified="2020-08-12" version="1">MFSTKSVLLFVVGVLIAYGVNRFYSQSTTTQTDGTMEDYKNAKSVYDFTVKDSQGADVSLEKYRGKVLLIVNIASQCGLTKGNYAELTELSQKYADKDFKILSFPCNQFGGQMPEGDGEEMVCHLRSAKAEVGDVFAKIDVNGDGAHPLYKYLKHKQGGTLGDSIKWNFAKFLVNKDGQPVDRYAPTTSPSSIVKDIDKLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VIJ4</accession>
    <name>A0A6E8VIJ4_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="EnsemblMetazoa" id="ACON004246-RC">
        <property type="protein sequence ID" value="ACON004246-PC"/>
        <property type="gene ID" value="ACON004246"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001195"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004246"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038935"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048666">
        <property type="term" value="P:neuron development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="CDD" id="cd14548">
        <property type="entry name" value="R3-PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12087">
        <property type="entry name" value="TM_EGFR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001137">
        <property type="entry name" value="Receptor protein-tyrosine phosphatase 10d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001460">
        <property type="entry name" value="Receptor protein-tyrosine phosphatase 10d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000916">
        <property type="entry name" value="Receptor-type tyrosine-protein phosphatase beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001177">
        <property type="entry name" value="Receptor-type tyrosine-protein phosphatase beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000009">
        <property type="entry name" value="Receptor-type tyrosine-protein phosphatase beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000823">
        <property type="entry name" value="Tyrosine-protein phosphatase 10D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001445">
        <property type="entry name" value="Tyrosine-protein phosphatase 10D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001589">
        <property type="entry name" value="Tyrosine-protein phosphatase 10D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001507">
        <property type="entry name" value="tyrosine-protein phosphatase 10D isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001334">
        <property type="entry name" value="tyrosine-protein phosphatase 10D isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001461">
        <property type="entry name" value="tyrosine-protein phosphatase 10D isoform X4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041201">
        <property type="entry name" value="PTPRJ_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050713">
        <property type="entry name" value="RTP_Phos/Ushers"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46957">
        <property type="entry name" value="CYTOKINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46957:SF3">
        <property type="entry name" value="CYTOKINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18861">
        <property type="entry name" value="PTP_tm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1222"/>
            <end position="1243"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="16">
        <location>
            <begin position="154"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="16">
        <location>
            <begin position="247"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="16">
        <location>
            <begin position="436"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="16">
        <location>
            <begin position="614"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="16">
        <location>
            <begin position="704"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="16">
        <location>
            <begin position="891"/>
            <end position="981"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="16">
        <location>
            <begin position="982"/>
            <end position="1081"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="14">
        <location>
            <begin position="1295"/>
            <end position="1550"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="15">
        <location>
            <begin position="1467"/>
            <end position="1541"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="20"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
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            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
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            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004246-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1578" mass="179717" checksum="DF7909BBB5967FF9" modified="2020-08-12" version="1">MQSTLERRKKMQPPTLHTHKQPERSDPATERRMGRRKLAAPGSMRLSSGLGLRELLLSSCLVVLLLAQQCYGADLVIEIPGNQGLDDSFYRLDYYPPIGNPAPNATIASRDVGDEIQFSNGLPGTRYNFWLYYTNSTHKDWLTWTVSITTAPDPPANLTVIPRSGKNVIINWSPPAQGNYSSFKLKILGLSDNFATNQTVAIEDNQFQYMVRDLTPGATYQVQAYTLYDGKESVAYTSRNFTTKPNTPGKFIVWFRNETTLLVLWQPPYPAGIYTHYKVSIEPPDALGSVLYVQKEGEPPGPAQAAFKGLVPGRAYNISVQTMSEDEISLPTTAQYRTVPLRPMNVTFDKKSITENSFKVMWEAPKGTSEFDKYQVSLSTSRRQQAVLRNDNENMAWLEFKDNLDPGKTYQVVVKTVSGKVTSWPASGDVTLKPLPVKQLQSYTDSKTGVITISWKPDELSTQDEYRISYHELETNNGDSSTMSTNQTSFALESLLPGRNYSVTVQALSRKMESNETVIFVVTRPSSPIIEDLKSIREGLNISWKSDVNSKQDKYEVTYTRNDTNDGKTVLTTESRLVFTNLYPGAGYEVKVFAVSHGLRSEPHSYFQAVYPNPPRNMTIEKVTSNSVLVHWKPPERSEFTEYSIRYRTESEKQWIRLPSVKATEADVTDMTPGEKYTIQVNTVSYGVESPNPQQVNQTVRPNPVSNIAPLVDSNNITLEFPRPEGRVETYIIHWWPTEQPEQVSMKNFTEVNTKPPLVRLLIGDLMSGVMYNFKIQTISYGLTSDLTKLQTRTMPLIQSEVVIVNNMHTRDMVTLSYTPTPQQSSKFDLYRFSLGDPSIPDKEKLANDTDRKVTFTGLTPGRLYNITVWTVSGKVSSQPIQRQDRMFPDPITMLEATSINDTWIALKWDIPKGEYTSFEVQYLMNDSHYVQNYTVNNHITITDLKPHRNYTITVVVRSGTESSVLRVSLPISANFQTKEALPGRMDKFAPIDIQPSEITFEWSLPPNEQNGIIRQFTITYGLDGSQHTQVKDFRPNELRGSIKALQPGKSYVFRIQAKTAIGYGPEHIWKQKMPILAPPKPETQVVPTEVGSSATTIEIRFRKHYFSDQNGVVTTYTIIIAEDDSKNASGLEMPSWRDVQSYSVWPPYQVIEPYYPFKNSSVEDFTIGTENCDAKKTGYCNGPLKSGTTYKVKVRAFTAPDKFTDTAYSYPIRTAQDNTSLIVSITVPLLIIAMLVGVVLFLRRRRHTGRKTTEQRTNDNMSLPDSTIETSRPVLVKNFAEHYRMMSADSDFRFSEEFEELKHIGRDQPCTFADLPCNRPKNRFTNILPYDHSRFKLQPVDDEEGSDYINANYVPGHNSPREFIVTQGPLHSTRDDFWRMCWESNSRAIVMLTRTFEKGREKCDHYWPHDTVPVYYGDIKVTLLNDSHYPDWVITEFMMTRGEQQRIIRHFHFTTWPDFGVPNPPQTLARFVRAFRERVGPDQRPIVVHCSAGVGRSGTFITLDRILQQIQVSDYVDIFGIVWAMRKERVWMVQTEQQYICIHQCLLVVLEGKEGTEREIHDNQGYEDDEGIAESGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VIK0</accession>
    <name>A0A6E8VIK0_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Translation elongation factor EF1B beta/delta subunit guanine nucleotide exchange domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the EF-1-beta/EF-1-delta family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040219363.2">
        <property type="nucleotide sequence ID" value="XM_040363429.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VIK0"/>
    <dbReference type="EnsemblMetazoa" id="ACON004235-RA">
        <property type="protein sequence ID" value="ACON004235-PA"/>
        <property type="gene ID" value="ACON004235"/>
    </dbReference>
    <dbReference type="GeneID" id="120947765"/>
    <dbReference type="CTD" id="34363"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001876"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004235"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039541"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005853">
        <property type="term" value="C:eukaryotic translation elongation factor 1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:translation elongation factor activity"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="EF1B"/>
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        <property type="entry name" value="Elongation factor 1-beta 1 like"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="eEF-1beta-like_sf"/>
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        <property type="entry name" value="EF-1_beta_acid_region_euk"/>
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        <property type="entry name" value="EF1B_bsu/dsu"/>
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        <property type="entry name" value="EF1B_bsu/dsu_GNE"/>
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        <property type="entry name" value="Transl_elong_EF1B/ribsomal_bS6"/>
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    <dbReference type="PANTHER" id="PTHR11595">
        <property type="entry name" value="EF-HAND AND COILED-COIL DOMAIN-CONTAINING FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11595:SF26">
        <property type="entry name" value="ELONGATION FACTOR 1-DELTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10587">
        <property type="entry name" value="EF-1_beta_acid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00736">
        <property type="entry name" value="EF1_GNE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01182">
        <property type="entry name" value="EF-1_beta_acid"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EF1_GNE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54984">
        <property type="entry name" value="eEF-1beta-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00824">
        <property type="entry name" value="EF1BD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00825">
        <property type="entry name" value="EF1BD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="3 4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Elongation factor 1 beta central acidic region eukaryote" evidence="7">
        <location>
            <begin position="146"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="Translation elongation factor EF1B beta/delta subunit guanine nucleotide exchange" evidence="6">
        <location>
            <begin position="182"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="110"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="114"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="137"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004235-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="268" mass="29348" checksum="20F3DAF4C691A7D0" modified="2020-08-12" version="1">MAAAAMAHEKLWTEKSTMDAAEKAYHEYMSKINSVPSQCTLASEISKARQHIKNSLERMDGIAALAASPGAELLDRLSSVEKENEKLRSIIDGLNNLVIDLHERVRSLESGSGKSAAPAKPAAAATKPAALAKKAEAEDDDDDVDLFGSEDEDEDKAAAELREKRLAEYAAKKSKKPALIAKSNVILDIKPWDDETDMKVMEQEVRKISADGLLLGAAKLVPLAYGIHKLQMSCVIEDDKISVDWLQEEIEKIEDYVQSVDIAAFNKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VIK4</accession>
    <name>A0A6E8VIK4_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">LIM zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VIK4"/>
    <dbReference type="EnsemblMetazoa" id="ACON004256-RA">
        <property type="protein sequence ID" value="ACON004256-PA"/>
        <property type="gene ID" value="ACON004256"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005217"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004256"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041553"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09400">
        <property type="entry name" value="LIM_like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022735">
        <property type="entry name" value="bMERB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050540">
        <property type="entry name" value="F-actin_Monoox_Mical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23167:SF84">
        <property type="entry name" value="ALPHA ACTININ 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23167">
        <property type="entry name" value="CALPONIN HOMOLOGY DOMAIN-CONTAINING PROTEIN DDB_G0272472-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12130">
        <property type="entry name" value="bMERB_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01203">
        <property type="entry name" value="DUF3585"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51848">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0440">LIM domain</keyword>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="6">
        <location>
            <begin position="60"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="BMERB" evidence="7">
        <location>
            <begin position="904"/>
            <end position="1066"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="123"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="202"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="357"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="702"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="816"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1070"/>
            <end position="1150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="129"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="218"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="231"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="374"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="429"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="450"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="461"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="511"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="537"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="580"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="609"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="648"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="703"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="723"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="837"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="850"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="875"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="1105"/>
            <end position="1122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="1141"/>
            <end position="1150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004256-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1150" mass="126461" checksum="879EA76EA8313144" modified="2020-08-12" version="1">MVKYEVPDRFSILTYLSQYYRVFSNQEPASKKVVAKDTKTDDLTSKEDSNTAFVGVPKRASCNRCSNPIFLAERISFGEKSYHRSCLKCARCGTQLTVGSFYETETDGEYCCETCPDEEIQLEQRRETSASPDGSSANVTGNGTPVAAAAAASIGLPKNVRSSKLLDRISFFESAPLSDEEKSSNLERKARMSHFLKESLKTVEQDNDEPPDLPLTGPPSSVTNDSLNGKEEEDTDDSDSDNVDDVEDEFEKLVQDLDEPDPDISKSLLADPVSPPRAVAIALEDTIEPNEQEQQKVAEVVLQTEVVVEESALEINSLEVQEKSELVTPVKVEDDSELTNNLPAEATVEAKEIIPLAPPVTEQTTTTTESSDLAPEHTIEDVVKNSEPKRTETKEQPSTEISNDDTPAADVEVIVENGSLALVPASESTTEHLDVPKENESTESLTTPSEESKNATESTERSNSMVPNETVESAVSESKVPVSVNKEETTIPTDTPEACNKVDNDEGLPAVSTQDGNEQEQYPSDLNPFGDEDDGEGREQIVELRKVPAGGKPVPTMRRVSTNPFGSEDEDEDVQQSPSSKPPRPPPPKVVQSPSAGGSSSKPVPANPFDEDDDDEPTDEPEIVPVSKPSPRRTPVPTPRKAHAYNDSISSLDNSLMSSSRLSSSNVSLTSSLESGPSSLIVPKRKKGKAPDIPLAVAGASTPIVSQQQQMGVSSAVRASVENLSNSSGSGTLTTPRKKRLAPAPPPIPNSSSTPKQSSTLQVREESGLTRVPSQRLLAVDASLLSNNDRDMSETMSRGTSNESVVYRRTIVPLVLDDDGPESPVHLDGTTTTSGRQWEKMKDNKEAQNRNRQSQSSPTSEATSNSFGSPGGNLSILSNKSSQGKWKRRKGPAPALPMMAAPLPERKPIKMLPLKEIHQELQIIETQQQGLEKQGIVLETMIRERCEGVEADVDLDIRLQPNSKEVEDLLMQLFELVNEKNELFRRQAELMYLRRMHRLEQEQADLEYEIRLLMAQPERNKTDSDKEKEEALITRLVEIVQLRNEVVECLEMDRIREAEEDLSIKQSIEERAASQQSKHSKKDSSASSTTLPLTDAEKRDSSTKLSKKEKKKLKEAKKLVKSKKIDSEKDADETESNTTKEKKKKRKFLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VIL3</accession>
    <name>A0A6E8VIL3_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RNA-binding protein NOB1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">May play a role in mRNA degradation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the NOB1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VIL3"/>
    <dbReference type="EnsemblMetazoa" id="ACON004064-RA">
        <property type="protein sequence ID" value="ACON004064-PA"/>
        <property type="gene ID" value="ACON004064"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001091"/>
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        <property type="component" value="Unplaced"/>
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        <property type="term" value="C:cytoplasm"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="C:preribosome, small subunit precursor"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="entry name" value="20S-pre-rRNA D-site endonuclease NOB1"/>
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        <property type="match status" value="1"/>
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    </dbReference>
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    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8 9" id="KW-0862">Zinc</keyword>
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        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="Nin one binding (NOB1) Zn-ribbon-like" evidence="11">
        <location>
            <begin position="264"/>
            <end position="325"/>
        </location>
    </feature>
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        <location>
            <begin position="113"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="135"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="175"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="274"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="277"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
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        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
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        <location>
            <position position="292"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
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            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023242"/>
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            <dbReference type="Pfam" id="PF08772"/>
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        <source>
            <dbReference type="Pfam" id="PF17146"/>
        </source>
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        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004064-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="414" mass="45673" checksum="1F74D9D8740B56AD" modified="2020-08-12" version="1">NYASNCYTVPGVMAEIRNNRQMKRLAVLPYNLQVKDPDPDVLAKVVAIAKKTGDYASLSLVDLQVIALTYELETIHVGRDHLRDEPLPSITVAAATKPDAFGGTQLLKGFYVPTKKSQSADESQEDDGENIGDISDDHSEAQEHETITAANDVQESSKESAPAGDTAETNVSEANDADNESCEEEYDDDDGEEEDGEDDEDGNDGDDDAGWITPSNIKQVKRDMGMDLHDETPTTVACITTDYALQNVLKQIGLQVAALDGRVIKQVRTYILRCYACFKTTPDATKVFCPKCGNRTLKKVSVSLDGEGKQIIHINNRRPLTAKYKNRPVAKFEGGKHAANPLLFADQPLPQQRISVKARSKTNALGDDYIAGYSPFVMRDVDSRSAVLRGSTNLKQRMSNYEYDNKRRGYKKPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VIM0</accession>
    <name>A0A6E8VIM0_ANOCL</name>
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        <submittedName>
            <fullName evidence="4">BSD domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VIM0"/>
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        <property type="protein sequence ID" value="ACON004273-PB"/>
        <property type="gene ID" value="ACON004273"/>
    </dbReference>
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    <dbReference type="VEuPathDB" id="VectorBase:ACON004273"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048172">
        <property type="term" value="P:regulation of short-term neuronal synaptic plasticity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:TORC2 signaling"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3970.10:FF:000001">
        <property type="entry name" value="synapse-associated protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BSD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BSD_dom"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR051494">
        <property type="entry name" value="BSD_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16019">
        <property type="entry name" value="SYNAPSE-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16019:SF6">
        <property type="entry name" value="SYNAPSE-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BSD"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="279"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
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            <begin position="15"/>
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            <end position="427"/>
        </location>
    </feature>
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        <location>
            <begin position="32"/>
            <end position="44"/>
        </location>
    </feature>
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            <begin position="360"/>
            <end position="412"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="417"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS50858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004273-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="470" mass="49990" checksum="61327CAC3FEE06E0" modified="2020-08-12" version="1">MFSGLTNQVTSWIGAAKGEPQDEEVPTPPNSAATTATATTSGVPQRRLQLQMQLPLQRVVMVVTKGYPTKGGVFSGVSSKVTGWLGNASIPSMPAIPTVSMPAMPAMPSMPSIPGLRKANQTDENGGITNEGLVTSPEKASQGIAGSTDAADEEDRSRYISATGGADSRPATGPGTPTEENAGQIGQVTHKVTAGAKSIGSFLYSSFNKAGDKIKHLKDNSILGEFSKEQEAFIKNQQGGGSAGACPWTGHANEAKIKEEILSLSADRRNFVRAPPAGVEFDFDYDSSYPVALAIMNDDKELEKMRFELVPKIITEENFWRNYFYRVSLICQAADLGTLGDNNEFVKRGASEDTEDTERTSSVGSMLNQSGDISSPATSPEEQTSASPLSPEQSPPAQTNTSDLQSGVTNAAASKPTHREFISDSFHTNEQDLEEVKAGMKKLGIDSLAQQANESIGLEHEKSGKKSKHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VIM3</accession>
    <name>A0A6E8VIM3_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="4">BSD domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VIM3"/>
    <dbReference type="EnsemblMetazoa" id="ACON004273-RC">
        <property type="protein sequence ID" value="ACON004273-PC"/>
        <property type="gene ID" value="ACON004273"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011394"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004273"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030309"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048172">
        <property type="term" value="P:regulation of short-term neuronal synaptic plasticity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038203">
        <property type="term" value="P:TORC2 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3970.10:FF:000001">
        <property type="entry name" value="synapse-associated protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3970.10">
        <property type="entry name" value="BSD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005607">
        <property type="entry name" value="BSD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035925">
        <property type="entry name" value="BSD_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051494">
        <property type="entry name" value="BSD_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16019">
        <property type="entry name" value="SYNAPSE-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16019:SF6">
        <property type="entry name" value="SYNAPSE-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03909">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00751">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140383">
        <property type="entry name" value="BSD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50858">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BSD" evidence="3">
        <location>
            <begin position="279"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="15"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="122"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="32"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004273-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="356" mass="37928" checksum="CFDC936F3150AB34" modified="2020-08-12" version="1">MFSGLTNQVTSWIGAAKGEPQDEEVPTPPNSAATTATATTSGVPQRRLQLQMQLPLQRVVMVVTKGYPTKGGVFSGVSSKVTGWLGNASIPSMPAIPTVSMPAMPAMPSMPSIPGLRKANQTDENGGITNEGLVTSPEKASQGIAGSTDAADEEDRSRYISATGGADSRPATGPGTPTEENAGQIGQVTHKVTAGAKSIGSFLYSSFNKAGDKIKHLKDNSILGEFSKEQEAFIKNQQGGGSAGACPWTGHANEAKIKEEILSLSADRRNFVRAPPAGVEFDFDYDSSYPVALAIMNDDKELEKMRFELVPKIITEENFWRNYFYRVSLICQAADLGTLGDNNEFVKRGASEDTEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VKL8</accession>
    <name>A0A6E8VKL8_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Sodium channel protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="22">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 18">Cell membrane</location>
            <topology evidence="1 18">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the sodium channel (TC 1.A.1.10) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="SMR" id="A0A6E8VKL8"/>
    <dbReference type="EnsemblMetazoa" id="ACON004707-RA">
        <property type="protein sequence ID" value="ACON004707-PA"/>
        <property type="gene ID" value="ACON004707"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010356"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004707"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034190"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001518">
        <property type="term" value="C:voltage-gated sodium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005248">
        <property type="term" value="F:voltage-gated sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0086010">
        <property type="term" value="P:membrane depolarization during action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019228">
        <property type="term" value="P:neuronal action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13433">
        <property type="entry name" value="Na_channel_gate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000061">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000046">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000047">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000019">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000022">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000023">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000026">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058542">
        <property type="entry name" value="IQ_SCN5A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001696">
        <property type="entry name" value="Na_channel_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044564">
        <property type="entry name" value="Na_chnl_inactivation_gate"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010526">
        <property type="entry name" value="Na_trans_assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024583">
        <property type="entry name" value="Na_trans_cytopl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043203">
        <property type="entry name" value="VGCC_Ca_Na"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10037:SF288">
        <property type="entry name" value="SODIUM CHANNEL PROTEIN PARA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10037">
        <property type="entry name" value="VOLTAGE-GATED CATION CHANNEL CALCIUM AND SODIUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24609">
        <property type="entry name" value="IQ_SCN5A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06512">
        <property type="entry name" value="Na_trans_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11933">
        <property type="entry name" value="Na_trans_cytopl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00170">
        <property type="entry name" value="NACHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="17 18" id="KW-0407">Ion channel</keyword>
    <keyword evidence="12 18" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0691">RNA editing</keyword>
    <keyword evidence="11 18" id="KW-0915">Sodium</keyword>
    <keyword evidence="3 18" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="16 18" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="7 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 18" id="KW-0813">Transport</keyword>
    <keyword evidence="9 18" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="133"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="204"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="238"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="262"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="393"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="762"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="806"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="835"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="890"/>
            <end position="912"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="970"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1270"/>
            <end position="1292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1298"/>
            <end position="1324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1345"/>
            <end position="1376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1463"/>
            <end position="1489"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1552"/>
            <end position="1570"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1582"/>
            <end position="1601"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1613"/>
            <end position="1632"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1676"/>
            <end position="1699"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1769"/>
            <end position="1789"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="21">
        <location>
            <begin position="1806"/>
            <end position="1841"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="34"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1101"/>
            <end position="1171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1956"/>
            <end position="2000"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="19">
        <location>
            <begin position="419"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="34"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="20">
        <location>
            <begin position="52"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="1128"/>
            <end position="1154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="20">
        <location>
            <begin position="1155"/>
            <end position="1171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="20">
        <location>
            <begin position="1968"/>
            <end position="1996"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU361132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004707-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="2064" mass="232009" checksum="C3C66AC279CE8489" modified="2020-08-12" version="1">MTEDSDSISEEERSLFRPFTRESLQAIEARIADEEAKQRELERKRAEGEIRYDDEDEDEGPQPDPTLEQGVPVPVRMQGSFPPELASTPLEDIDSFYSNQRTFVVISKGKDIFRFSATNALYVLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILQPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKEFPLDGSWGNLTDESWELFNSNDTNWFYSESGDIPLCGNSSGAGQCDEGYICLQGYGKNPNYGYTSFDTFGWAFLSAFRLMTQDYWENLYQLVLRSAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEALREAEEAAAAKAAKLEAQQAAAAAAANPEIAKSPSDFSCHSYELFVGQEKGNDDNNKEKMSIRSEGLESVSEITRTTAPTATAAGTAKARKVSAFTIRNGRGRFVGVPGSDRKPLVLSTYLDAQEHLPYADDSNAVTPMSEENGAIIVPVYYANLGSRHSSYTSHQSRISYTSHGDLLGGMTKESRLRNRSARNTNHSIVPPPNANNLSYAETNHKGQRDFDLTQDCTDDAGKIKHNDNPFIEPAQTQTVVDMKDVMVLNDIIEQAAGRHSRASDHGVSVYYFPTEDDDEDGPTFKDKALEFLMKMIDIFCVWDCCWVWLKFQEWVAFIVFDPFVELFITLCIVVNTLFMALDHHDMDPDMEKALKSGNYFFTATFAIEATMKLIAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYVDNVDRFPDHDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMLVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNETNKIAEAFNRISRFSNWIKMNLANALKFVKNKLTSQIASVQPTEHGENELELTPDDILADGLLKKGIKEHNQLEVAIGDGMEFTIHGDLKNKAKKNKQIMNNSKDDDTASIKSYGSHKNRPFKDESHKGSAETMEGEEKRDASKEDLGIDEELDDEGEGDEGPLDGELIIHAEEDEVIEDSPADCCPDNCYKKFPVLAGDDDAPFWQGWGNLRLKTFQLIENKYFETAVITMILLSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFIIVMVSLINFVASLCGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCVDKNKTTLPHEIIPDVNACKAENYSWENSPMNFDHVGKAYLCLFQVATFKGWIQIMNDAIDSRDVGKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTNKKFDMIIMLFIGFNMLTMTLDHYKQSETFSAVLDYLNMIFICIFSSECLMKIFALRYHYFIEPWNLFDFVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKDKSGLDDVYNFKTFGQSMILLFQMSTSAGWDGVLDGIINEEDCLPPDNDKGYPGNCGSSTIGITYLLAYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPDGTQYVRYDQLSDFLDVLEPPLQIHKPNRYKIISMDIPICRGDMMFCVDILDALTKDFFARKGNPIEETAELGEVQQRPDEVGYEPVSSTLWRQREEYCARLIQHAWKRYKQRHGGGTDASGDDLEIDACDNGCGGGNGNENDDSGDGATGSGDNGSQHGGGSISGGGGTPGGGKSKGIIGSTQANIGIVDSNISPKESPDSIGDPQGRQTAVLVESDGFVTKNGHRVVIHSRSPSITSRTADV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VKN8</accession>
    <name>A0A6E8VKN8_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the DEAD box helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="RefSeq" id="XP_040221050.1">
        <property type="nucleotide sequence ID" value="XM_040365116.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VKN8"/>
    <dbReference type="EnsemblMetazoa" id="ACON004912-RB">
        <property type="protein sequence ID" value="ACON004912-PB"/>
        <property type="gene ID" value="ACON004912"/>
    </dbReference>
    <dbReference type="GeneID" id="120948595"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001600"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004912"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_029210"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17966">
        <property type="entry name" value="DEADc_DDX5_DDX17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000008">
        <property type="entry name" value="ATP-dependent RNA helicase RhlB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000079">
        <property type="entry name" value="probable ATP-dependent RNA helicase DDX17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 8" id="KW-0347">Helicase</keyword>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="12">
        <location>
            <begin position="78"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="109"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="11">
        <location>
            <begin position="312"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="460"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="577"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="7">
        <location>
            <begin position="78"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="464"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="519"/>
            <end position="535"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004912-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="679" mass="74784" checksum="33C6D101C0936944" modified="2020-08-12" version="1">MRRNDRNGGRVEKRFDRMERNGENLRSIRWDQVKLEAFQKNFFQPASSVLTRSRAEVNQYLDKNEITVYGKDVPAPIMHFHESGFPQYMLDEFQRQAFKEPTFIQAVGWSIAMSGRDMVGIAKTGSGKTLAYILPALVHISNQPRIARGDGPIALVLAPTRELAQQIKQVCDDFGRRMGIYNTCVFGGASKYPQESDLRRGVEIVIATPGRLIDFLERETTNLRRCTYLVLDEADRMLDMGFEPQIRKIISQIRPDRQVLMWSATWPKEIRKLAEEFLRDYIQINIGSLNLAANENILQIIDCCEEYEKENRLFKLLEQISSQNDGKTIIFVETKRKVDKIVNVIRRQGWRADGIHGDKSQKDRDYVLNTFRRSTNGILVATDVASRGLDVDDVKFVINFDFPNNTEDYVHRIGRTGRSTNKGTSYTFFTPANSSKAPDLITVLQDANQYINPELHEYARGSGRYRGSGGGRSRGNMSGGRGMGGRMGGGSRFSDSRSGRPNDGGYRNGNDGRGGASYPRRDDFGRSDRDHRDGGSRSGASYGATGAARAPNGVGTNGYGASGTPAAYGSDDRGYGGHYGSVSQPQAHYGASGYPAQGSVARRPESDRSRVPASAAIGLPVTPAMPSAIAYAHPGMALSAAPYGAGVGYQYAAPPPTAMPRTDGYPPRGVIPSMATGGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VKN9</accession>
    <name>A0A6E8VKN9_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Potassium channel domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the two pore domain potassium channel (TC 1.A.1.8) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VKN9"/>
    <dbReference type="EnsemblMetazoa" id="ACON004717-RA">
        <property type="protein sequence ID" value="ACON004717-PA"/>
        <property type="gene ID" value="ACON004717"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005457"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004717"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030172"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015271">
        <property type="term" value="F:outward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022841">
        <property type="term" value="F:potassium ion leak channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030322">
        <property type="term" value="P:stabilization of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003280">
        <property type="entry name" value="2pore_dom_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11003:SF334">
        <property type="entry name" value="FI03418P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11003">
        <property type="entry name" value="POTASSIUM CHANNEL, SUBFAMILY K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01333">
        <property type="entry name" value="2POREKCHANEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5 8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="47"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="144"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="177"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="413"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="11">
        <location>
            <begin position="143"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="11">
        <location>
            <begin position="421"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="221"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="295"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="346"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="231"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="306"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF07885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004717-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="457" mass="52326" checksum="802E7E73339B0C2C" modified="2020-08-12" version="1">MSSRRTAPPPMQQLQPQMSISYGEPEKKPRSKCMSFVCCMWKVFTCIFSHVTLVATVVAYCFLGAFTFEHLEAENERNVKKGISSIRVNLTDAIWKMTNDRPVLHQQNWTSAAIMHLQSFEKEILTAMKKDGWDGNEDVDQIQWTFFGALFYSIIVITTIGYGHIAPKTYMGKISTIFYAILGIPLMLLCLSNIGDIMASSFRFLYWRVCCYVCTREPKRSNSRRSRSSRGTVRQGSVQAAGTNLRRSVRNSQRSADSGFDPYYEPGLSHSYSDTDYMDDDSPCKTPILCNKYALDDVDDQDGRSTRPNPRSQSMPRQTGRYGDRLMPDRMPYYTEDNEIAENMQSVRKSAPQRRREPRPVPAPSPRIMSPMGFAVHRQARHLQNVIDDNSLYDDEWDLHSGELPPSSIVRPVPIWLCVFLVVSYIIAGAFMFSEWEEWSFLDSAYFCFITLTTIGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VKP6</accession>
    <name>A0A6E8VKP6_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Palmitoyltransferase</fullName>
            <ecNumber evidence="7">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="7"/>
    <dbReference type="RefSeq" id="XP_040224430.1">
        <property type="nucleotide sequence ID" value="XM_040368496.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VKP6"/>
    <dbReference type="EnsemblMetazoa" id="ACON004938-RB">
        <property type="protein sequence ID" value="ACON004938-PB"/>
        <property type="gene ID" value="ACON004938"/>
    </dbReference>
    <dbReference type="GeneID" id="120950464"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010046"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004938"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037245"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12246">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="34"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="63"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="185"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="220"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="8">
        <location>
            <begin position="141"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004938-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="319" mass="37236" checksum="96EC4FFAC85DD74E" modified="2020-08-12" version="1">MMSMPHGQSYDYHRTTFDGERNERCGCCVRTMKWFPVLFIASVIGWSYYAFVIQLSFFTVTNIVQRILFLLFYHAVLVMFLWSYYQTVFTDIGRVPSRFHVPRAELDRIVRATNEVEQKEILETFAKELPVVTRTLNASVRFCDKCRLIKPDRAHHCSVCGVCVLKLDHHCPWVNNCINFTNYKYFILFLGYALLYCVYVACSTIPYMELLWTGKIDGRFHILFLFFVSVMFAISLVSLFGYHVYLVLLNRTTLESFRTPIFRFGGPDKNGFSLGKLNNFQEVFGDDWRLWFVPVYTSLGDGIVFPCRDVAPYSIEFAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VKP7</accession>
    <name>A0A6E8VKP7_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">CBFD_NFYB_HMF domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VKP7"/>
    <dbReference type="EnsemblMetazoa" id="ACON004965-RA">
        <property type="protein sequence ID" value="ACON004965-PA"/>
        <property type="gene ID" value="ACON004965"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009688"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004965"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031955"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004965-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="135" mass="14409" checksum="13594BAE14760414" modified="2020-08-12" version="1">MEEAQYSEDLFSTQDEAAFGNANSEQEAPTHKSTEEKDVPAQPGKDTASAAEKEAGASAEQETPDLVFSEDIDFGKESSEKGDNSSSNAGVTSDNFEEPMEPDVNVENADERTETEEVVESSVPNPNATDAANTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VKP8</accession>
    <name>A0A6E8VKP8_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Serine-threonine kinase receptor-associated protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the WD repeat STRAP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VKP8"/>
    <dbReference type="EnsemblMetazoa" id="ACON005029-RA">
        <property type="protein sequence ID" value="ACON005029-PA"/>
        <property type="gene ID" value="ACON005029"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004551"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005029"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033871"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032797">
        <property type="term" value="C:SMN complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000387">
        <property type="term" value="P:spliceosomal snRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19877">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19877:SF13">
        <property type="entry name" value="SERINE-THREONINE KINASE RECEPTOR-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 7" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="17"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="58"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="101"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="224"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="308"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="311"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00040390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005029-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="332" mass="35553" checksum="45B4E4BBD4B46020" modified="2020-08-12" version="1">GKPMLRMGDTGDWVGTFEGHKGAVWGVALNESATLAASGAADFTGKIWNAVTGEEVHSLQHNHIVKTVAFSHDSQYLVTGSNEKLVRVFDLNSEGKALESYAGHAGAVKRALFCRNEKCVISCADDKSMRLWDRGTGQEVSRVEFSSHPNGLELSKDGSILTVTYGNCTAFYDMETLTQLKEITIPTRVSSASLHPDKHIFVCGGEDFKMYKYDYITGNEIESFKGHFGPVHSVSFSPDGELYASGSEDGTLRLWQTTVGKTYGLWKCTEPTDLNNSTSNNSAANNTTANSAVSGVVAPVTPNNAAVSCPPVSSATAPVTPTPSANREVTAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VKQ3</accession>
    <name>A0A6E8VKQ3_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">SP-RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">Ngousso</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein sumoylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PIAS family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VKQ3"/>
    <dbReference type="EnsemblMetazoa" id="ACON005031-RB">
        <property type="protein sequence ID" value="ACON005031-PB"/>
        <property type="gene ID" value="ACON005031"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011942"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005031"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042048"/>
    <dbReference type="UniPathway" id="UPA00886"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061665">
        <property type="term" value="F:SUMO ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097240">
        <property type="term" value="P:chromosome attachment to the nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16790">
        <property type="entry name" value="SP-RING_PIAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.780:FF:000001">
        <property type="entry name" value="E3 SUMO-protein ligase PIAS2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000247">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.780">
        <property type="entry name" value="PINIT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023321">
        <property type="entry name" value="PINIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038654">
        <property type="entry name" value="PINIT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004181">
        <property type="entry name" value="Znf_MIZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10782:SF94">
        <property type="entry name" value="SUPPRESSOR OF VARIEGATION 2-10, ISOFORM I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10782">
        <property type="entry name" value="ZINC FINGER MIZ DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14324">
        <property type="entry name" value="PINIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02891">
        <property type="entry name" value="zf-MIZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51466">
        <property type="entry name" value="PINIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51044">
        <property type="entry name" value="ZF_SP_RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PINIT" evidence="13">
        <location>
            <begin position="27"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="SP-RING-type" evidence="12">
        <location>
            <begin position="225"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="354"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="361"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="373"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="404"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="454"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005031-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="512" mass="55624" checksum="8A85189DA4AC6799" modified="2020-08-12" version="1">MYQQQYANAVQTDNRVGQVHANGSMAVPGYGDYPIHPDVQLKKLAFFDVMATLLKPATLVPSNTAQRVQEGSYFFHLTPQQANEIALNRDISNSAKIEHNVQVQLRFCLLETTCEQDDHFPPNIVLKVNNKLCPLPNPIPTNKPGVEPKRPPRPVNITAQVKLSPTVANHISVSWCTEYNRGYAVACYLVRKLSSAQLLQRLKTKGVKAADYTRALIKEKLNEDADCEIATTMLKVSLICPLGKMRMTTPCRSSTCSHLQCFDASLYLQMNERKPTWNCPVCDKPAVYNNLVIDGYFQEVLVSTKLSSEDTEIQLHKDGSWSTRVKLNDSDDGSPSKAVQKVEVISDDIEVITTDPPKPIVSQTSVISTNEPTSTTTSSETVDLTLSDSDDDAPAKRKKISARTNGTGSSNNPTSSSSSSSTSAAGSSSTSAGAVTRSKVNEDPSSSVIMLDSPSPPSTPNPPHNGSNDVSSTTTPSLAAFHYHQQQESPGGSGGSNEQMSNFQINPLALHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VL00</accession>
    <name>A0A6E8VL00_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VL00"/>
    <dbReference type="EnsemblMetazoa" id="ACON005064-RA">
        <property type="protein sequence ID" value="ACON005064-PA"/>
        <property type="gene ID" value="ACON005064"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000576"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005064"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032052"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="213"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="387"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="414"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="213"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="224"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="387"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="414"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005064-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="472" mass="54685" checksum="0B4BA7035457C33F" modified="2020-08-12" version="1">MQLVKEQQRVNVYQFSRSEEEEIDEAAFKCLKYDMIKEIIPRIGKRAKFLQRYEEYKENLTERPAFAELQLEEKDRFSPTSPQKPSRIDWGRIEKVCGPVPIYLKNLLKMSGYEAESSLRNLNDDDFIDMERFAREELIHLVPATQFYFHIFCSDPAREFRLVPGHRKLLLQISAYLNYTKVASEIQRPEPALRLPADLTIQREREREHRLLLAQQQHQQQQQHQKEQQHQQKEHQQQKEQQQLLQQQQQLLQEVVRMHQQPLTEIQEQMLSEMKMSHKNSPLAFEQATVFPPLSDEERIKKIQGCIVKLIDMWSEKQPIKLQITEEMINVTYETNGYNAFIKCPMCAVQIKVSKLPTKKGDRFVISNFSKHIIKMHFSQLSDGAGGGGGGVGGNGVGGHAGSVGSERHLSMDNVSSYYGGENSDSNTYPYDDYGSGKRPKLDEDDDEMDPVPPHLLHPYVSIKDEIIDLDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VL01</accession>
    <name>A0A6E8VL01_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Palmitoyltransferase</fullName>
            <ecNumber evidence="10">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="10">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="10"/>
    <dbReference type="RefSeq" id="XP_040226552.2">
        <property type="nucleotide sequence ID" value="XM_040370618.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VL01"/>
    <dbReference type="EnsemblMetazoa" id="ACON005111-RA">
        <property type="protein sequence ID" value="ACON005111-PA"/>
        <property type="gene ID" value="ACON005111"/>
    </dbReference>
    <dbReference type="GeneID" id="120951735"/>
    <dbReference type="CTD" id="351"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007373"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005111"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031467"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883:SF43">
        <property type="entry name" value="PALMITOYLTRANSFERASE APP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883">
        <property type="entry name" value="ZINC FINGER DHHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0564">Palmitate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="64"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="180"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="224"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="12">
        <location>
            <begin position="134"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="445"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="571"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="445"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="605"/>
            <end position="622"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005111-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="809" mass="86832" checksum="3A81854B65DE5EDF" modified="2020-08-12" version="1">MAPSQKVTKKWEIFAGRNKFFCDGYLMTAPNSGVFYFTVILITATSGLFFVFDCPFLAQRITPAIPIIGGILFVFTLSSLFRTAFSDPGIIPRASQDEAAYIEKQIEVPNSLNSPTYRPPPRTKEVFVKGQTVKLKYCFTCKIFRPPRASHCSLCDNCVDRFDHHCPWVGNCVGKRNYRFFYMFIVSLAFLAVFIFSCTTTHIVMLLKEDNQFIDVVKRTPSSVIIAIICFCSVWSVIGLAGFHTYLTTSDQTTNEDIKGSFSSKGGQQAINPYSQGNICLNCFHILCGPITPSLIDRRGVVTDEYRTQMQATEKYNSATVPPLVVMQPGMDTLNKHYSMEHELQNASNGGGVGGTGVYRQRSYDNLQNDKSNSVAHLVENEAPLSTTGNVSILSGGAGGGLEDTTTTTVLLDEDGMELETRGIGGGPSMDDSSNQLTGSYTNLFRDSMSNGVANSPSGGGQPGKPNNSYHHHSSMMMSECDIIQSADEIINVSELMMVCSVSGHENVYSNVPPAQISPSGSAVGLLASPKRASVGSSNVSGVVDVSSSTVGPGGYQHVYSNVTSPCGPAAAEQLPTGVSTGTGGISGSQQQQQQQHIHPALGNGKHHQQQQHHQHHHHPHHQLALDDPSQLLSSTFISNDLDLDDPVLSSSFATATKSTANTMTKLHPPPAPSSAAGGPMVAAQQQHLKQPQAMITTIEMKNVIKTLDPNVIIQQQASSNGSSGTTAAVCSSANVIDSNGGIASMHNNTTTTNNNNSHTESPMFISPSASRMRLLQESTMIDTALDLDSLDGSIGNHSQSCLVKTAIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VL15</accession>
    <name>A0A6E8VL15_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Endophilin-A</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_040219596.1">
        <property type="nucleotide sequence ID" value="XM_040363662.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VL15"/>
    <dbReference type="EnsemblMetazoa" id="ACON005076-RB">
        <property type="protein sequence ID" value="ACON005076-PB"/>
        <property type="gene ID" value="ACON005076"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON005076-RC">
        <property type="protein sequence ID" value="ACON005076-PC"/>
        <property type="gene ID" value="ACON005076"/>
    </dbReference>
    <dbReference type="GeneID" id="120947867"/>
    <dbReference type="CTD" id="273"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000727"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005076"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030880"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11790">
        <property type="entry name" value="SH3_Amphiphysin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000013">
        <property type="entry name" value="Amphiphysin isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000172">
        <property type="entry name" value="Amphiphysin, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003005">
        <property type="entry name" value="Amphiphysin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004148">
        <property type="entry name" value="BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46514">
        <property type="entry name" value="AMPHIPHYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46514:SF3">
        <property type="entry name" value="AMPHIPHYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03114">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01251">
        <property type="entry name" value="AMPHIPHYSIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00721">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51021">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="BAR" evidence="10">
        <location>
            <begin position="24"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="9">
        <location>
            <begin position="293"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="334"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005076-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="357" mass="40559" checksum="D7C5084F030E6B42" modified="2020-08-12" version="1">MAENKGMLLAKSVQKHAGRAKEKLLQNLGKVDRTADEIFDEHLTNFNRQHTCATRLQKEFSNYIRCVRAVQNASKSLMEAITEVYESQWTGSEVLYGQAKTIDTQYQQFSYKLADQVLKQLDTYALQFPEMKKKIDKRGRKLVDYDSQRHSFQSLQANAAKRKDDLKVTRGREQLEEAKSTYEVLNSELHDELPALYDSRILFLVTNLQTLFACEQQFHSETSKVYAELEAIVDKLATESQRGSYTLKKINANSNPSSPQQSPVKANLSIVNNVTNGSANANGRAATTDLPPGVLYRVKATYKYVREDVDELSFEVGDVINVVEYEDPEDQEEGWLMGNKEGTNEKGMFPANFTRPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VL19</accession>
    <name>A0A6E8VL19_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Guanine nucleotide-binding protein subunit alpha</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the G-alpha family. G(q) subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040227258.1">
        <property type="nucleotide sequence ID" value="XM_040371324.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VL19"/>
    <dbReference type="EnsemblMetazoa" id="ACON005079-RB">
        <property type="protein sequence ID" value="ACON005079-PB"/>
        <property type="gene ID" value="ACON005079"/>
    </dbReference>
    <dbReference type="GeneID" id="120952153"/>
    <dbReference type="CTD" id="36384"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007505"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005079"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032935"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005834">
        <property type="term" value="C:heterotrimeric G-protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001664">
        <property type="term" value="F:G protein-coupled receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031683">
        <property type="term" value="F:G-protein beta/gamma-subunit complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007188">
        <property type="term" value="P:adenylate cyclase-modulating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050793">
        <property type="term" value="P:regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00066">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.400.10:FF:000002">
        <property type="entry name" value="guanine nucleotide-binding protein G(Q) subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003977">
        <property type="entry name" value="Guanine nucleotide-binding protein G(q) subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000692">
        <property type="entry name" value="Guanine nucleotide-binding protein subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.400.10">
        <property type="entry name" value="GI Alpha 1, domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000654">
        <property type="entry name" value="Gprotein_alpha_Q"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001019">
        <property type="entry name" value="Gprotein_alpha_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011025">
        <property type="entry name" value="GproteinA_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218">
        <property type="entry name" value="GTP-BINDING PROTEIN ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218:SF329">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN G(Q) SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00503">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00318">
        <property type="entry name" value="GPROTEINA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00442">
        <property type="entry name" value="GPROTEINAQ"/>
    </dbReference>
    <dbReference type="SMART" id="SM00275">
        <property type="entry name" value="G_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47895">
        <property type="entry name" value="Transducin (alpha subunit), insertion domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51882">
        <property type="entry name" value="G_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9 11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4 10 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 9 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0564">Palmitate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0807">Transducer</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="43"/>
            <end position="48"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="149"/>
            <end position="150"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="174"/>
            <end position="180"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="199"/>
            <end position="203"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="268"/>
            <end position="271"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="325"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU369122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005079-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="353" mass="41728" checksum="A155397BA5565787" modified="2020-08-12" version="1">MECCLSEEAKEQKRINQEIERQLRRDKRDARRELKLLLLGTGESGKSTFIKQMRIIHGSGYSDEDKRGFIKLVYQNIFMAMQSMIRAMDLLKILYSDPANIEHAELIRSVDFETVTTFEPPYVQAIKDLWADAGIQECYDRRREYQLTDSAKYYLMEIDRVAAPNYLPTEQDILRVRVPTTGIIEYPFDLEEIRFRMVDVGGQRSERRKWIHCFENVTSIIFLVALSEYDQILFESENENRMEESKALFKTIITYPWFQHSSVILFLNKKDLLEEKIMYSHLVDYFPEYDGPKHDDVKAREFILKVYLRENPDPDRMCYSHFTCATDTENIKLVFCAVKDTIMQTALKEFNLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VL20</accession>
    <name>A0A6E8VL20_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Aldehyde dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4 7">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VL20"/>
    <dbReference type="EnsemblMetazoa" id="ACON005124-RF">
        <property type="protein sequence ID" value="ACON005124-PF"/>
        <property type="gene ID" value="ACON005124"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006213"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005124"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031069"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004029">
        <property type="term" value="F:aldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006081">
        <property type="term" value="P:aldehyde metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07132">
        <property type="entry name" value="ALDH_F3AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000003">
        <property type="entry name" value="Aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000004">
        <property type="entry name" value="Aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012394">
        <property type="entry name" value="Aldehyde_DH_NAD(P)"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43570">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43570:SF16">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE TYPE III, ISOFORM Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036492">
        <property type="entry name" value="ALDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="2 4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="469"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="9">
        <location>
            <begin position="7"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="active site" evidence="5 6">
        <location>
            <position position="209"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR036492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR036492-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005124-PF"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="494" mass="54883" checksum="6673E5C68F4B4C56" modified="2020-08-12" version="1">MSFSDIVQQLRTTFNSGKTRDVDFRLNQLKALLRCYEENTAEMANVLAADLRKHKQEAHLLEIEFILNDLRNTLYNLREWVKPEKPEKSLVNVMDGVYIYKDPYGVVLVIGAWNYPLQLTLVPVAAAIAAGNCVVIKPSEVAPASAKFIAETLPKYLSQDCYRVVTGGPKETSELLKEKFDYVFYTGSGRVGKIIHQACSENLTPCTLELGGKSPCYLDGTANIAIAARRILWGKFINAGQTCIAPDYILCSKQVQKQFLEEARKVLNEWYGANPKDSPDLCRIINQNHFQRLTSLLKGANVAIGGETDLQDRYISPTILIDVKSTDPVMQDEIFGPILPILNVDNAYDAIKFINSSAKPLCLYIFSKNVTDQRLMIENTSSGGVCVNDTLMHCAVEALPFGGVGPSGMGSYHGKYSFDTFVHKKSCLTKDFNPIGEKLAASRYPPYSESKLSFLSTLLKKRQGINLHFLPYLLMFGIGVASTLVVSTILKDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VL27</accession>
    <name>A0A6E8VL27_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein eL22</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">60S ribosomal protein L22</fullName>
        </alternativeName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL22 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VL27"/>
    <dbReference type="EnsemblMetazoa" id="ACON005046-RB">
        <property type="protein sequence ID" value="ACON005046-PB"/>
        <property type="gene ID" value="ACON005046"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007574"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005046"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034759"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.210:FF:000001">
        <property type="entry name" value="60S ribosomal protein L22 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002671">
        <property type="entry name" value="Ribosomal_eL22"/>
    </dbReference>
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        <property type="entry name" value="Ribosomal_eL22_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10064">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L22"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR10064:SF0">
        <property type="entry name" value="FI24544P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ribosomal_L22e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00040613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00041214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005046-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="94" mass="11415" checksum="5C7511A004EC7030" modified="2020-08-12" version="1">MDVADFEKYLKERFKVNGKAGNLGSNVSFERQKMKVYVNSDVHFSKRYLKYLTRKYLKKNSLRDWIRVVSNDKDLYELRYFRISSNDDDEEENE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VMG0</accession>
    <name>A0A6E8VMG0_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="3">MSP domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VMG0"/>
    <dbReference type="EnsemblMetazoa" id="ACON005556-RB">
        <property type="protein sequence ID" value="ACON005556-PB"/>
        <property type="gene ID" value="ACON005556"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_003590"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005556"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037101"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140284">
        <property type="term" value="C:endoplasmic reticulum-endosome membrane contact site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053012">
        <property type="entry name" value="ER-organelle_contact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000535">
        <property type="entry name" value="MSP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008962">
        <property type="entry name" value="PapD-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46384">
        <property type="entry name" value="MOTILE SPERM DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46384:SF1">
        <property type="entry name" value="MOTILE SPERM DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00635">
        <property type="entry name" value="Motile_Sperm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49354">
        <property type="entry name" value="PapD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50202">
        <property type="entry name" value="MSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="212"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="MSP" evidence="2">
        <location>
            <begin position="18"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005556-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="250" mass="27679" checksum="11B80618ADC1C411" modified="2020-08-12" version="1">MLDKPSTTFQPESSEGEMLRIKPQNTITFSRLGNDLVGTVEITNVDTKDVSYKVKTTAPEKFRVRPSTGVLPPSASVNINVMLQQGQQMHTINREKFLVMCIGLTDEISTNPQDLTELWKKISAKSSSVEQHRLKCALPANCDESGLESMFGGAGNVIAGPDQYGVMSGSFGGSSMMGMGADKQMSLLQQTISHLNETTHRLEVQVKRNHRFQWITCVLFVLLALAIVYILKIEIKSHASEYCTTAERNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VMP2</accession>
    <name>A0A6E8VMP2_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ITPR-interacting domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_040221348.2">
        <property type="nucleotide sequence ID" value="XM_040365414.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VMP2"/>
    <dbReference type="EnsemblMetazoa" id="ACON005635-RB">
        <property type="protein sequence ID" value="ACON005635-PB"/>
        <property type="gene ID" value="ACON005635"/>
    </dbReference>
    <dbReference type="GeneID" id="120948735"/>
    <dbReference type="CTD" id="37039"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011936"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005635"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035541"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029325">
        <property type="entry name" value="ITPR-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043444">
        <property type="entry name" value="TESPA1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17469:SF15">
        <property type="entry name" value="ITPR-INTERACTING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17469">
        <property type="entry name" value="SPERM SPECIFIC ANTIGEN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14722">
        <property type="entry name" value="KRAP_IP3R_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01257">
        <property type="entry name" value="KRAP_IP3R_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ITPR-interacting" evidence="2">
        <location>
            <begin position="511"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="182"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="268"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="384"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="444"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="633"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="184"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="210"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="274"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="311"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="444"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="459"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="483"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="517"/>
            <end position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005635-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="668" mass="72637" checksum="DB597E6D3B520A5B" modified="2020-08-12" version="1">MRKKSPASVQKWVDSIQIPSGAGAGEQSTGSDAQIATGQDNDPSAYVSNYTRSDQLAAGTVPQTEGHDRPDRGWTGATETGEPLETDSEEERQAFAYEHQQQQSDQPDRVLIDSDQNKHSSTSSTTNHGLPSEPPISSLATSTPKAIKPDEGAKQMPVSEGAAPSGLSIRNFLSKKSLLGKFNRSGSNLEQSPPTTTTTTAGEGVAEMGNPEHVEHGRHSTEKGEVTAAPSPFVVGKSKINEFYDKLNMNKAKYGLMKAKNLDIRNMLSGGSRESADARKAHEAQPVQSGADEPNPVESGFEGELEPPASESEHERSVSPQHHEPLEVSVVDVDPTDPYEFSFDESFPKSDELIPGMISEEDEEEKENILDGASVLPGYGYDAYLNPPKPKTHRMGIGRIGRSSSENPRATARRYNLMEIGRSFSEMNDDDDSFAISECNNSCPNPSSLNTSSSINEHGSVSNLTRSVPVSPIPRTSSKLSIQQDHPSQHQLHQQQQPLHQQQSSPRRRSTMLVKDSSLQSDSSRCSSVESLLNARKPDPEKILLNLGFGPAPHSTDVLAKIPKRFLKPSQVRGVDTEAFLRQQQLSMHIHENSVLGYRGLVGNPHIPPSMIVAKIMERFQENDSKTRLTAAGVPLLQPDSRNSSGPPSPVPMSRHASLATADGRLKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VMR5</accession>
    <name>A0A6E8VMR5_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Facilitated trehalose transporter Tret1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">High-capacity facilitative transporter for trehalose. Does not transport maltose, sucrose or lactose. Mediates the bidirectional transfer of trehalose. Responsible for the transport of trehalose synthesized in the fat body and the incorporation of trehalose into other tissues that require a carbon source, thereby regulating trehalose levels in the hemolymph.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Trehalose transporter subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040228924.2">
        <property type="nucleotide sequence ID" value="XM_040372990.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040228925.2">
        <property type="nucleotide sequence ID" value="XM_040372991.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VMR5"/>
    <dbReference type="SMR" id="A0A6E8VMR5"/>
    <dbReference type="EnsemblMetazoa" id="ACON005563-RA">
        <property type="protein sequence ID" value="ACON005563-PA"/>
        <property type="gene ID" value="ACON005563"/>
    </dbReference>
    <dbReference type="GeneID" id="120953225"/>
    <dbReference type="CTD" id="36248"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004826"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005563"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_036350"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051119">
        <property type="term" value="F:sugar transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015574">
        <property type="term" value="F:trehalose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17358">
        <property type="entry name" value="MFS_GLUT6_8_Class3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000055">
        <property type="entry name" value="Facilitated trehalose transporter Tret1-2 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044775">
        <property type="entry name" value="MFS_ERD6/Tret1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050549">
        <property type="entry name" value="MFS_Trehalose_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48021">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48021:SF96">
        <property type="entry name" value="FACILITATED TREHALOSE TRANSPORTER TRET1-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="30"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="71"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="103"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="131"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="160"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="185"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="270"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="310"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="334"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="368"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="404"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="436"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="14">
        <location>
            <begin position="32"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00060205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00069106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005563-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="490" mass="53035" checksum="A41971C382DEAE1E" modified="2020-08-12" version="1">MVKILMRADTHVSFSVPIEEPVAKCTFSQVLAALSVSLGSMVVGFSSAYTSPALVSMKDRNITSFEVTDQSGSWVGGIMPLAGLAGGILGGPMIEYLGRKNTILATATPFIISWLLIGCATHVAMVLVGRALSGLCVGIASLSLPVYLGETVQPEVRGTLGLLPTAFGNIGILLCFVAGKYLDWSGLAFLGAALPIPFLLLMFLIPETPRWYVSRNREDRARKALQWLRGRKADVEPELKGISKSHQDAERHASSSAMLDLLNKANLKPLLISLGLMFFQQLSGINAVIFYTVQIFQSAGSTIDEKLCTIIVGVVNFIATFIATVLIDRLGRKILLYISDVAMIITLMTLGTFFYMKNNGDDVSEIGWLPLAAFVVFVVGFSLGFGPIPWLMMGEILPGKIRGSAASVATAFNWSCTFVVTKTFADITASIGNHGAFWMFGSICIVGLLFVIVYVPETQGKSLEDIERKMMGRVRRMSSVANIKPLSFNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VN61</accession>
    <name>A0A6E8VN61_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Alpha-tubulin N-acetyltransferase</fullName>
            <shortName evidence="5">Alpha-TAT</shortName>
            <shortName evidence="5">TAT</shortName>
            <ecNumber evidence="4 5">2.3.1.108</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Acetyltransferase mec-17 homolog</fullName>
        </alternativeName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Specifically acetylates 'Lys-40' in alpha-tubulin on the lumenal side of microtubules. Promotes microtubule destabilization and accelerates microtubule dynamics; this activity may be independent of acetylation activity. Acetylates alpha-tubulin with a slow enzymatic rate, due to a catalytic site that is not optimized for acetyl transfer. Enters the microtubule through each end and diffuses quickly throughout the lumen of microtubules. Acetylates only long/old microtubules because of its slow acetylation rate since it does not have time to act on dynamically unstable microtubules before the enzyme is released.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 5">
            <text>L-lysyl-[alpha-tubulin] + acetyl-CoA = N(6)-acetyl-L-lysyl-[alpha-tubulin] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15277"/>
            <dbReference type="Rhea" id="RHEA-COMP:11278"/>
            <dbReference type="Rhea" id="RHEA-COMP:11279"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.108"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the acetyltransferase ATAT1 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.108" evidence="4 5"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8VN61"/>
    <dbReference type="EnsemblMetazoa" id="ACON005828-RA">
        <property type="protein sequence ID" value="ACON005828-PA"/>
        <property type="gene ID" value="ACON005828"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002467"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005828"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_029974"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019799">
        <property type="term" value="F:tubulin N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048666">
        <property type="term" value="P:neuron development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070507">
        <property type="term" value="P:regulation of microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000060">
        <property type="entry name" value="Alpha-tubulin N-acetyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03130">
        <property type="entry name" value="mec17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038746">
        <property type="entry name" value="Atat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007965">
        <property type="entry name" value="GNAT_ATAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12327">
        <property type="entry name" value="ALPHA-TUBULIN N-ACETYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12327:SF0">
        <property type="entry name" value="ALPHA-TUBULIN N-ACETYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05301">
        <property type="entry name" value="Acetyltransf_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51730">
        <property type="entry name" value="GNAT_ATAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="6">
        <location>
            <begin position="1"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="120"/>
            <end position="133"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="156"/>
            <end position="165"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="site" description="Crucial for catalytic activity" evidence="5">
        <location>
            <position position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00051998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00066570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005828-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="206" mass="23322" checksum="0C9987073221BF78" modified="2020-08-12" version="1">MEFRFNMHPLFRARIVRINNSLLPTGFVAQSRRVALDATAQISEIINTIGSMSAQAQGLSVPVTTAQKLRNSDHHIYLMFESNDRNGLVVGILKVGRKSLYVFDPSGETVNVTAPCVLDFYVHESRQRGGLGRELFEHMLREENIQPDELAIDRPSEKLLGFLQKHYGLSKKIPQMNNFVVYEGFFASKAQNSTDIDGRRMHITAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VN62</accession>
    <name>A0A6E8VN62_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ETS domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the ETS family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040225454.1">
        <property type="nucleotide sequence ID" value="XM_040369520.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VN62"/>
    <dbReference type="EnsemblMetazoa" id="ACON005755-RB">
        <property type="protein sequence ID" value="ACON005755-PB"/>
        <property type="gene ID" value="ACON005755"/>
    </dbReference>
    <dbReference type="GeneID" id="120951037"/>
    <dbReference type="KEGG" id="acoz:120951037"/>
    <dbReference type="CTD" id="38700"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006969"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005755"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030599"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000343">
        <property type="entry name" value="Ets at 65A, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000418">
        <property type="entry name" value="Ets_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046328">
        <property type="entry name" value="ETS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11849:SF304">
        <property type="entry name" value="DNA-BINDING PROTEIN D-ETS-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11849">
        <property type="entry name" value="ETS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00178">
        <property type="entry name" value="Ets"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00454">
        <property type="entry name" value="ETSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00413">
        <property type="entry name" value="ETS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00345">
        <property type="entry name" value="ETS_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00346">
        <property type="entry name" value="ETS_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50061">
        <property type="entry name" value="ETS_DOMAIN_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ETS" evidence="6">
        <location>
            <begin position="65"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005755-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="237" mass="26495" checksum="7D4F1DF6DBE78B25" modified="2020-08-12" version="1">MIDIKSSPDYLNRSSGGSFGNFSMLFADSSYKSSWGSHSSSQSQDPYQMFGPTSSRLASSGSGQIQLWQFLLELLSDSTNATCITWEGTNGEFKLTDPDEVARRWGERKSKPNMNYDKLSRALRYYYDKNIMTKVHGKRYAYKFDFQGLAAATQPQTDPTYKYQSDLFMSPYHHGAKLSSFMSPHHSMTSSSASIFPSAASWGNWGSPATNLYPSHTMSHVTPSHVAPHLGSYPHYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VP72</accession>
    <name>A0A6E8VP72_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Gustatory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Gustatory receptor which mediates acceptance or avoidance behavior, depending on its substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the insect chemoreceptor superfamily. Gustatory receptor (GR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VP72"/>
    <dbReference type="EnsemblMetazoa" id="ACON006143-RA">
        <property type="protein sequence ID" value="ACON006143-PA"/>
        <property type="gene ID" value="ACON006143"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACON006143"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032593"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007635">
        <property type="term" value="P:chemosensory behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008049">
        <property type="term" value="P:male courtship behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050909">
        <property type="term" value="P:sensory perception of taste"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013604">
        <property type="entry name" value="7TM_chemorcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21143:SF133">
        <property type="entry name" value="GUSTATORY AND PHEROMONE RECEPTOR 32A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21143">
        <property type="entry name" value="INVERTEBRATE GUSTATORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08395">
        <property type="entry name" value="7tm_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="40"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="154"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="221"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="252"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="332"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON006143-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="357" mass="41071" checksum="847BA7842877F613" modified="2020-08-12" version="1">MLKVLNIFHTLLICNVRTVRPTKGHQHAVGYKISRARVQLLLPLVVILVLAFLNPWTTHRRKDQHTISLNLFTIYARLLVTVLIVVLLICDNFRQRFHLLSLLHTIPGSAFTGRTGSQFSVKKLQLVILLICNVLSISEALYKIYQSRQYSYSLGVFSGVLVEHYILLNLLLCQLLAETLSNAYATLQHSLRVRPFHAIISELTVLDGCKNHLSEVIGLKLILVILHLMFNVSFCTYDILEHVLSEKHLRDITRLSIIAVQETVMLFGLCFQFTMLDQKEAELKDALKSMQYTNLKRQSRDQKDFYDLVNLKLMMESPKITACGLFEINLQIFYNVFAAIITYIVILFQFRGFEKSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VP80</accession>
    <name>A0A6E8VP80_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Gustatory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Gustatory receptor which mediates acceptance or avoidance behavior, depending on its substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the insect chemoreceptor superfamily. Gustatory receptor (GR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_049463713.1">
        <property type="nucleotide sequence ID" value="XM_049607756.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VP80"/>
    <dbReference type="EnsemblMetazoa" id="ACON006143-RF">
        <property type="protein sequence ID" value="ACON006143-PF"/>
        <property type="gene ID" value="ACON006143"/>
    </dbReference>
    <dbReference type="GeneID" id="125906883"/>
    <dbReference type="CTD" id="117472"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON006143"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032593"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007635">
        <property type="term" value="P:chemosensory behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008049">
        <property type="term" value="P:male courtship behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050909">
        <property type="term" value="P:sensory perception of taste"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013604">
        <property type="entry name" value="7TM_chemorcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21143:SF133">
        <property type="entry name" value="GUSTATORY AND PHEROMONE RECEPTOR 32A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21143">
        <property type="entry name" value="INVERTEBRATE GUSTATORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08395">
        <property type="entry name" value="7tm_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="34"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="128"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="162"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="228"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="257"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="340"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON006143-PF"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="365" mass="42806" checksum="722D5689C56C1CDE" modified="2020-08-12" version="1">MNHLKVLNLFNHLFISLVYVDQTPKGPTVRRKNALRNIIILLATVSIVSFFGLKRTLTDFRQMISSVTDIVHLLKYFINADIMYTILSGMYQHSLKVIVIVNEAIAIDYTMQRVCTLMKNRFAIRHTLLKMLTGAIMLLRGSFFLMYLYLQFRYNISYELYLVLHANLLVELSMDLQKLFLLYFALYMIQRYRILANLLDRSDPTTMAVVFKVFEDLITFKKHLSKTFGLHLLALILQTFVACSIHAYLIVVEQRHIFQQIANVAQLVINLTLFFILTYYYDCVEIKEAELKDALKSMQYTNLKRQSRDQKDFYDLVNLKLMMESPKITACGLFEINLQIFYNVFAAIITYIVILFQFRGFEKSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VP90</accession>
    <name>A0A6E8VP90_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="6">PDZ domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VP90"/>
    <dbReference type="EnsemblMetazoa" id="ACON006260-RA">
        <property type="protein sequence ID" value="ACON006260-PA"/>
        <property type="gene ID" value="ACON006260"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004821"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON006260"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032404"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031941">
        <property type="term" value="C:filamentous actin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001725">
        <property type="term" value="C:stress fiber"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051371">
        <property type="term" value="F:muscle alpha-actinin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061061">
        <property type="term" value="P:muscle structure development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050604">
        <property type="entry name" value="PDZ-LIM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24214">
        <property type="entry name" value="PDZ AND LIM DOMAIN PROTEIN ZASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24214:SF55">
        <property type="entry name" value="Z BAND ALTERNATIVELY SPLICED PDZ-MOTIF PROTEIN 66, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13180">
        <property type="entry name" value="PDZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0440">LIM domain</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PDZ" evidence="5">
        <location>
            <begin position="33"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="96"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="170"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="229"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON006260-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="263" mass="29693" checksum="EF1BE6CB009DB1FE" modified="2020-08-12" version="1">MEYDRQQFERPAFWRLPKQKTFELPYGRKRVHFSEQVQDHSPAQPELLRGDVITKIDQYDARDLMHIDAQNLFRNAGNQIKVTVRRDDKVALHQSAHPINGNPVAPLSPANFAQPYQPYAEPGQPAKPQLHQNFPPPDPTQLLPSTATSLPHGPQSYAAALERPVETLPHTVFPGVDGTGAYHLPKQPYPPPAPTSLNDANEAITNQPYRTTPLVLPGAKVPKKDTLPTESYLRHHPNPAMRAPPAHDYTDSLMKQKVQQYWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VR96</accession>
    <name>A0A6E8VR96_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Calponin-homology (CH) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the filamin family.</text>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON007006-RC">
        <property type="protein sequence ID" value="ACON007006-PC"/>
        <property type="gene ID" value="ACON007006"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_013864"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007006"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031100"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21227">
        <property type="entry name" value="CH_jitterbug-like_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21229">
        <property type="entry name" value="CH_jitterbug-like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001473">
        <property type="entry name" value="Jitterbug, isoform C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001349">
        <property type="entry name" value="Jitterbug, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001551">
        <property type="entry name" value="Jitterbug, isoform M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000068">
        <property type="entry name" value="Putative Filamin-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000078">
        <property type="entry name" value="Putative Filamin-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001589">
        <property type="entry name" value="Actinin_actin-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044801">
        <property type="entry name" value="Filamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017868">
        <property type="entry name" value="Filamin/ABP280_repeat-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001298">
        <property type="entry name" value="Filamin/ABP280_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38537">
        <property type="entry name" value="JITTERBUG, ISOFORM N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38537:SF13">
        <property type="entry name" value="JITTERBUG, ISOFORM N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00630">
        <property type="entry name" value="Filamin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00557">
        <property type="entry name" value="IG_FLMN"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00019">
        <property type="entry name" value="ACTININ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50194">
        <property type="entry name" value="FILAMIN_REPEAT"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="6">
        <location>
            <begin position="57"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="6">
        <location>
            <begin position="178"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="4">
        <location>
            <begin position="395"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="4">
        <location>
            <begin position="478"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="4">
        <location>
            <begin position="574"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="4">
        <location>
            <begin position="1047"/>
            <end position="1144"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="4">
        <location>
            <begin position="1147"/>
            <end position="1231"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="4">
        <location>
            <begin position="1230"/>
            <end position="1327"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="4">
        <location>
            <begin position="1325"/>
            <end position="1418"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="4">
        <location>
            <begin position="1428"/>
            <end position="1507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="654"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="849"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="677"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="707"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="727"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="765"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="854"/>
            <end position="863"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007006-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1566" mass="169792" checksum="9F98E337CB67378B" modified="2020-08-12" version="1">MELPKGAKITHTGLLQHTPDGKTELQKITQAGLVARSPEGTAAKGMNIRGNEDLWVEIQANTFKNWVNEHLRESGLQVNILHLSPQVIEFHEDFCDGTLLCALVEGLQKRPLKPSWNKRPANQHHYLENVTTALNAIEADGVKLVNIGNVDIVNGNVKLILGLIWSLIVRYQIGRSKFPPRKLMLAWLQAALPDCKVSNLTTDWNSGVLLSALLDYCEPGLFPHWRTLNQSESVRNCERAMNLAYERFGIPKVLEPEYLASRWLDELSGMTYLSYFMRPGGPGYNATMKWVNGQIKRPVTNFTSDFNDGKVFCEIIKDLGGPVPDPHKLSSDPSQWESNQQKVIDGGLKLGVKPVLAAKDMATADEEHLGVMAYTTWLRWVVPRPPLANMLTVQLESTSGRVGEPTEFRVEALSRDVDMSRVRAYVGMPNTNTLQQVRLGARGEGTFVPDKYGMHEIVLEIDDNQLGGHFFRVLPRLVHVAPPGMAPCALGSLVEVLVNATGAPKTEDILVTAYSPSGRPLKCPLKKIEEGHSAIFKPDEAGVWEIAITYQGRHIQGGPFTCAVFDPSGVSVHGLDGAMPLRAHSFEVDARGVGVAGELHVDIVHEKRSLVCSVERLQENKYQVTFMPRSNGKHRVYVYFNGYDVKGSPFIMKVGSKGRSGKTRTSPHQHDGKLRSESPSYHFNASSLTRKADTQSGSLRREIYSPPTVQKSGSRSPQDYEPGGYIKSTSKETYTSRVMSPARHSPSPKLLFPATETDYSGGFRRSTELLSQRRDSDELKSSPVRLGGGDEAASSYVKTIRTENHTTKTIRTVLDGESSPIAHIRASPGLKSPQTVVSATLLDTRTASPKPLITTTSTSTSSTRYIPSPVTEQRVASPIAMHEKSNGTYKVTERNSTYKSTVTRDVVRDSPTLERVVRSPLTTAAGTSVVDYSSNIKVNGANGQPSRRDSYDVINKTKHLFSQNSLESLANLTERQLNTDLTYDRSTLDNQTEKNTHFNKFSLGTETQPRRPVIEDEGLYRAGGYLKNYRTESNESRYESKYSSQLKGGFEPIGRDVSGARAIRVQDIPDGVLGRPVEFEIDGSQAGSGNLEILVNGGRVTSAVRALGNQRFIASFTPHESGTHTVQITFNEETVPGSPWNINIMSSPGLTALGESTRLVPANLPAVFEVLPPPGASIRGSDCVATVLSPSKTKVSARVTHESANGSLRIEFVPSEVGTHIIEASIGGTTLVGGPLIAKVYDSSLIQVTDVNGGVVGQPCQFRVDASAAGEGQLEISINEGEVPNHVQVVGGGRCLVSFTPEQAKPHLIDIKFNGETVIGCPFVCSVADTSRVLLNLTNLELIPVNRPASFHITVSGGGAAELAVSVRGPQGELPVRVTGDIHAGFTAEFTPNHVGAHTINVEYNGYPVQGTPFVAKSYDATKVAVGSVSKGTVGRPVQFTVDAGDAGEGNLEITISAKGHNIPTQVHPQGNAKFAVSFVPAEPCEHIINVSFNKMLVPGCPITVIINGGTTGPQVSLGGPGPLHMPNSLVINHAGGRLEDIEVNVEGRWLPPVVPGTVDTSVVFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VTA4</accession>
    <name>A0A6E8VTA4_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">glycogenin glucosyltransferase</fullName>
            <ecNumber evidence="2">2.4.1.186</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 8 family. Glycogenin subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.186" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8VTA4"/>
    <dbReference type="EnsemblMetazoa" id="ACON007724-RC">
        <property type="protein sequence ID" value="ACON007724-PC"/>
        <property type="gene ID" value="ACON007724"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_008034"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007724"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039185"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008466">
        <property type="term" value="F:glycogenin glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02537">
        <property type="entry name" value="GT8_Glycogenin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000085">
        <property type="entry name" value="Glycogenin, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002495">
        <property type="entry name" value="Glyco_trans_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050587">
        <property type="entry name" value="GNT1/Glycosyltrans_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11183">
        <property type="entry name" value="GLYCOGENIN SUBFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01501">
        <property type="entry name" value="Glyco_transf_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="326"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="330"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="357"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007724-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="382" mass="43326" checksum="D9BB973667212186" modified="2020-08-12" version="1">MSKYAWVTLATNDSYSLGALVVAHSLKRVHTEHQTAVLITPGVSESMKTKLRAVFNVVEEVNLLDSKDEANLALLKRPELGVTFTKLHCWRLTQFEKCVFLDADTLVLRNCDELFEREELSAAPDIGWPDCFNSGVYVYTPNMETFSSLVQYAVTHGSFDGGDQGLLNSYFSDWAHKDIQKHLPFIYNTSSVATYSYLPAFKQFGQNTKILHFIGVAKPWLQNFNSETRKVYVPSECQHLANFLQYWWDIFAEDVHSRLSPDMSGIAGALAQLHLGEAKSKEQEAYEEHMRRQCWETGNIDYMGRDSFDNIWKRIQQTINTGSCMEESSEASVAQTTAPGTTQRASRRPSRSRSATPKRDPKDRSPTPQPEEKISVDKKEGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VTB1</accession>
    <name>A0A6E8VTB1_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Glycerol-3-phosphate dehydrogenase [NAD(+)]</fullName>
            <ecNumber evidence="14">1.1.1.8</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 14">
            <text>sn-glycerol 3-phosphate + NAD(+) = dihydroxyacetone phosphate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11092"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.8"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Phospholipid metabolism; alpha-glycerophosphate cycle.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.8" evidence="14"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8VTB1"/>
    <dbReference type="EnsemblMetazoa" id="ACON007593-RB">
        <property type="protein sequence ID" value="ACON007593-PB"/>
        <property type="gene ID" value="ACON007593"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009997"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007593"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038450"/>
    <dbReference type="UniPathway" id="UPA00086"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141152">
        <property type="term" value="F:glycerol-3-phosphate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046168">
        <property type="term" value="P:glycerol-3-phosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006650">
        <property type="term" value="P:glycerophospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
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        <property type="entry name" value="Glycerol-3-phosphate dehydrogenase [NAD(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Glycerol-3-phosphate dehydrogenase [NAD(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
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        <property type="entry name" value="6PGD_dom2"/>
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    <dbReference type="InterPro" id="IPR006168">
        <property type="entry name" value="G3P_DH_NAD-dep"/>
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        <property type="entry name" value="G3P_DH_NAD-dep_C"/>
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        <property type="entry name" value="G3P_DH_NAD-dep_euk"/>
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        <property type="entry name" value="G3P_DH_NAD-dep_N"/>
    </dbReference>
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        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
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        <property type="entry name" value="glycerol3P_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11728">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11728:SF8">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE DEHYDROGENASE [NAD(+)]-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07479">
        <property type="entry name" value="NAD_Gly3P_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01210">
        <property type="entry name" value="NAD_Gly3P_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000114">
        <property type="entry name" value="Glycerol-3-P_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00077">
        <property type="entry name" value="GPDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00957">
        <property type="entry name" value="NAD_G3PDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 12 13" id="KW-0520">NAD</keyword>
    <keyword evidence="7 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycerol-3-phosphate dehydrogenase NAD-dependent N-terminal" evidence="15">
        <location>
            <begin position="7"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="Glycerol-3-phosphate dehydrogenase NAD-dependent C-terminal" evidence="16">
        <location>
            <begin position="195"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="206"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="11"/>
            <end position="16"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
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        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
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    <feature type="binding site" evidence="12">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
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    <feature type="binding site" evidence="11">
        <location>
            <begin position="270"/>
            <end position="271"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="297"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
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    <feature type="binding site" evidence="12">
        <location>
            <position position="299"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011009"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00048683"/>
        </source>
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        <source>
            <dbReference type="PIRSR" id="PIRSR000114-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000114-2"/>
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    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR000114-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU361243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF07479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007593-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="350" mass="38061" checksum="C3D1A40B8F17F671" modified="2020-08-12" version="1">MSDKVRVCIVGSGNWGSAIAKIVGANAKRLATFEDRVTMYVYEEMIDGKKLTEIINTTHENVKYLPGHKLPENVVAVPDVVEAAKDADILIFVVPHQFIRGLGTQLLGKIKSTAVGLSLIKGFDVAEGGGMELISHLITKHLKIPCSVLMGANLAGEVAEEKFCETTIGCRDMKIAQTLRDLFLTPNFRVVVVDDVDAVEICGALKNIVACGAGFVDGMGLGDNTKAAVIRLGLMEMIKFVDVFYPGSKLSTFFESCGVADLITTCYGGRNRKVSEAFVKTGKTIVELENEMLNGQKLQGPITAEEVNFMLKSKGMEDKFPLFTAIHKICTGTVKPQGFLDCLRNHPEHM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VV83</accession>
    <name>A0A6E8VV83_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="7">RRM domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VV83"/>
    <dbReference type="EnsemblMetazoa" id="ACON008433-RB">
        <property type="protein sequence ID" value="ACON008433-PB"/>
        <property type="gene ID" value="ACON008433"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001768"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008433"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033099"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12283">
        <property type="entry name" value="RRM1_RBM39_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12284">
        <property type="entry name" value="RRM2_RBM23_RBM39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12285">
        <property type="entry name" value="RRM3_RBM39_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000080">
        <property type="entry name" value="RNA-binding protein 39 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000090">
        <property type="entry name" value="RNA-binding protein 39 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029123">
        <property type="entry name" value="RBM39_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006509">
        <property type="entry name" value="RBM39_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01622">
        <property type="entry name" value="SF-CC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48036">
        <property type="entry name" value="SPLICING FACTOR (PAD-1), PUTATIVE (AFU_ORTHOLOGUE AFUA_1G15810)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15519">
        <property type="entry name" value="RBM39linker"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="228"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="326"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="38"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="46"/>
            <end position="55"/>
        </location>
    </feature>
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        <location>
            <begin position="66"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="110"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="189"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="211"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008433-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="583" mass="66224" checksum="32CDBF8704B89050" modified="2020-08-12" version="1">MPIARMHHFHFLEYDLIFSVRFTTCPICVCDDAAASPVENGATTNGEKKSKENGHSKSSRHRSRSRERERERERDRDRERDRDRERERDRERDRDGEKASKKHRSGSPGRTRDKERRRSKDRSKSRSPARRDRSKDRSKEKDRGKSDHHRREVVVEKRRSRDRVDHRRRSRERDYRRRSRSRDGGRGMGRGRRSMSPKPYRGRGRGGSGYYRDRSPLEEMSQEDRDARTVFCMQLSQRIHARDLEEFFSSVGKVRDVRLITCNKTKRFKGIAYIEFKDPESVALALGLSGQKLLGIPISVQHTQAEKNRMASQPPVAPPKNPSGPMRLYVGSLHFNITEDMLNGIFEPFGKIDNIQLIMDADTGRSKGYGFITFHNADDAKKALEQLNGFELAGRPMKVGNVTERLDVTTHASLDTDEMDRSGIELGATGRLQLMFKLAEGAGLAVPRAAADALLATAPQPVPQQPIMQSPPIATQCFLLSNMFDPATETNPNWDLEIQDDVIEECNKHGGVQHVYVDKQSPSGNVYVKCPSIATAVLAVNALHGRWFAGRVIGAAYVPLINYYNLFPEAAQSVTMLQPKRSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VV84</accession>
    <name>A0A6E8VV84_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Menorin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the menorin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040236090.2">
        <property type="nucleotide sequence ID" value="XM_040380156.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VV84"/>
    <dbReference type="EnsemblMetazoa" id="ACON007860-RA">
        <property type="protein sequence ID" value="ACON007860-PA"/>
        <property type="gene ID" value="ACON007860"/>
    </dbReference>
    <dbReference type="GeneID" id="120957795"/>
    <dbReference type="KEGG" id="acoz:120957795"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_014028"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007860"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033271"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019356">
        <property type="entry name" value="Menorin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21184:SF6">
        <property type="entry name" value="CONSERVED PLASMA MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21184">
        <property type="entry name" value="MENORIN (DENDRITIC BRANCHING PROTEIN)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10223">
        <property type="entry name" value="Menorin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026060001" description="Menorin-like domain-containing protein" evidence="2">
        <location>
            <begin position="23"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="Menorin-like" evidence="3">
        <location>
            <begin position="25"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00044953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007860-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="305" mass="33713" checksum="F37ECAA5FFFA5892" modified="2020-08-12" version="1">MVPSLLLCSLLVLSTASSVADAKANLTKITWAHAVNDAEFLTRVLASDVQFIEADISLGYLKDDVNKEREIPIMAHPPATESDISLETFLQRILQFNREANDPSKVKGVKLDFKSIGAFERSIDIIRASYDMTAFDTWINADILPGPVDNTATVPVDPTRFFTAARRLGKATLSIGWTTRWGPDFSEGLYTEAQVDAMIAAIRANGIDKVGNAITFPVRGGIVANSVTNMIRLFCALKDTNNVTFTIWSSADDAVNVEKLREFIFTTGLDRTYVDVPDDLHKKLHLDENAFSNTCKKTLQARCFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VVC5</accession>
    <name>A0A6E8VVC5_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Dual specificity protein phosphatase</fullName>
            <ecNumber evidence="7">3.1.3.16</ecNumber>
            <ecNumber evidence="7">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Dual specificity phosphatase able to dephosphorylate phosphotyrosine, phosphoserine and phosphothreonine residues, with a preference for phosphotyrosine as a substrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="7"/>
    <dbReference type="EC" id="3.1.3.48" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8VVC5"/>
    <dbReference type="EnsemblMetazoa" id="ACON008228-RA">
        <property type="protein sequence ID" value="ACON008228-PA"/>
        <property type="gene ID" value="ACON008228"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002602"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008228"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031759"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033549">
        <property type="term" value="F:MAP kinase phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008138">
        <property type="term" value="F:protein tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14515">
        <property type="entry name" value="DUSP3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020405">
        <property type="entry name" value="Atypical_DUSP_subfamA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45682">
        <property type="entry name" value="AGAP008228-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45682:SF5">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01908">
        <property type="entry name" value="ADSPHPHTASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01909">
        <property type="entry name" value="ADSPHPHTASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="8">
        <location>
            <begin position="38"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="9">
        <location>
            <begin position="109"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphocysteine intermediate" evidence="6">
        <location>
            <position position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR620405-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008228-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="193" mass="22157" checksum="C2E3B60C66EBC70A" modified="2023-06-28" version="2">MAEQTTGRQLQRILHYSVTPCRPMLGLRRSECALYDVDCDEVYPRLYIGDANSAKNKQYLRLIGVTHVLNTAEGTRFGQVDTGHSYYRDMSGIRYMGFPMIDQPTTDISRYFYIASKFIENGINSGGKVLVHCMMGMSRSATCVLAYLMIARKMTAAEAIRTVRMHRDIRPNEGFLQQLADLDNELKRDRLYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VVD0</accession>
    <name>A0A6E8VVD0_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON008482-RA">
        <property type="protein sequence ID" value="ACON008482-PA"/>
        <property type="gene ID" value="ACON008482"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011973"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008482"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039842"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000791">
        <property type="term" value="C:euchromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140658">
        <property type="term" value="F:ATP-dependent chromatin remodeler activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034728">
        <property type="term" value="P:nucleosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18668">
        <property type="entry name" value="CD1_tandem_CHD5-9_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18663">
        <property type="entry name" value="CD2_tandem_CHD5-9_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17995">
        <property type="entry name" value="DEXHc_CHD6_7_8_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.40:FF:000001">
        <property type="entry name" value="chromodomain-helicase-DNA-binding protein 8 isoform X4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000003">
        <property type="entry name" value="chromodomain-helicase-DNA-binding protein 8 isoform X4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000015">
        <property type="entry name" value="chromodomain-helicase-DNA-binding protein 9 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.5.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
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            <dbReference type="EnsemblMetazoa" id="ACON008482-PA"/>
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            <dbReference type="Proteomes" id="UP001105220"/>
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    <sequence length="6877" mass="725686" checksum="AB6C889A22C0E99A" modified="2020-08-12" version="1">[encoded data, 6877 characters]</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VVD5</accession>
    <name>A0A6E8VVD5_ANOCL</name>
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        <submittedName>
            <fullName evidence="5">Choline kinase N-terminal domain-containing protein</fullName>
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        <name type="scientific">Anopheles coluzzii</name>
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            <taxon>Nematocera</taxon>
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            <taxon>Anophelinae</taxon>
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        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
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    <reference evidence="5" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
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                <consortium name="EnsemblMetazoa"/>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="5">Ngousso</strain>
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        <text evidence="3">Belongs to the choline/ethanolamine kinase family.</text>
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</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VXG9</accession>
    <name>A0A6E8VXG9_ANOCL</name>
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        <submittedName>
            <fullName evidence="18">Piezo-type mechanosensitive ion channel component</fullName>
        </submittedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">Ngousso</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PIEZO (TC 1.A.75) family.</text>
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        <property type="protein sequence ID" value="ACON009276-PB"/>
        <property type="gene ID" value="ACON009276"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:mechanosensitive monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Piezo"/>
    </dbReference>
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        <property type="entry name" value="Piezo_cap_dom"/>
    </dbReference>
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        <property type="entry name" value="Piezo_THU9_anchor"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Piezo_TM25-28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056768">
        <property type="entry name" value="THU_Piezo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47049">
        <property type="entry name" value="PIEZO-TYPE MECHANOSENSITIVE ION CHANNEL HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47049:SF2">
        <property type="entry name" value="PIEZO-TYPE MECHANOSENSITIVE ION CHANNEL HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12166">
        <property type="entry name" value="Piezo_cap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24874">
        <property type="entry name" value="Piezo_THU9_anchor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24871">
        <property type="entry name" value="Piezo_TM1-24"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15917">
        <property type="entry name" value="Piezo_TM25-28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23188">
        <property type="entry name" value="THU_Piezo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="30"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
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            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="115"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="193"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="220"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="244"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="316"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
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            <end position="522"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="534"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
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            <end position="736"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
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            <end position="990"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
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            <end position="1057"/>
        </location>
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    <sequence length="2673" mass="305633" checksum="FFFA98E919ED4C82" modified="2020-08-12" version="1">MANYFLCLVVQRLVLPLILATCVLMRPVGLAIPYLICLLVIPYVPVANKKTIQGPTGLFMRLLILFVVLDLAAVIVFQLILISFPGSSVESCSLGEIILRHIGLVSFNGLGVWMVVYWLAPEAILLVGAVTVHIVLRKLTGVIVPRRRRRRRGADGNTAEDDTEQGADAEGEGPAAEAEESELQTSLISPERLPFFTTLGVLLSMCMLCVVGIIQPSAISGVYFVVFLGAASWWGCYKKLDRAFGIVLKIVLVVLVIHIVLLLAYQNPWPQEVLYFNDTAARVLALKPILNSSCNLANGTDIRDVHFNSDLDTDVYLNPIFLLLCYYTLTMTSSLLLRTRSDTTKDRKRLDLSSQLENGIRLQNQISFRMQRKNILRKTLTSDRWRGAARKVKSQSNGPSARGYYTYGRSTTYGRGNSTINTDIPDERTPLMRRGTHRIRDASANDGKDCAQTDQIALESLEEEEPSSFFEQALLAIRNVASYIYKNSYIFTNVIMMVWSIMYHSWLTFVLLLWANLIWILPNHRKNMLNSSPFLVIYAEVLLIAQFLYGMNLTEAELPTVVEGSGINLEQLGFVRRTEYPIVPLLVKSLFTTMFWISLRQLIQEKQTERRESMIANMAAPLQLPVGAATTAATGPPSATERLPEKKSSAIVDRMAKFFRSFMIKFWIWVVALTLFLSAIIGNRMTVFRIVYMVLFLCFLLSFQVWKEIDVWRRVMFTFWITVILYSMAILILVYLHQFEQFPRYWAAIGISKELQEDIGLEIFKTGQLFLHLVNPTLVVIITVIQLHYCHDRFLIISEIPSIQTETEQSDEEDKQEPVAPATDGPAYGTFTNADNGAIMDSTEAVPSATDTTVGQKADAQPAREKLPLEEDDDDEEQIEKIEDFRFRKLSRQEITGFARKAWKMALDLLELTWLFFEIHLLKIVLIVAFSLSVNSVNFLHLLYVVLSVFTVKAHTSGKDSLTYIYQIRITRIMSLFSAIMLIITMIYQVKYIKEGDFQSECPNIDANTTLMDMNNAKWIGMRKTGSGETLSDLLRPYLVYILLVTVHQVIILRQTIHRMRLGRSPKTPSLMFPNIVRADADKDIPRMIKYLFNYGYYKFGIEISLVALIVVVGNRMDVLSIFYAIWLAVMFHMTRDGLQRLWRWLTWFIVAVIPIQYVLFVGLPPFACVNYPWFVPQLDHFRIWAMLPENSYEFRSFANKMVSDFVLLMFLCRQTVVFRLEREPPRRFGGGSNQSVLGDFQKLDEGNFQNPTPDFITKVRNWLDMLKRTLFLVSFWFTLAVVFLSGSSRVNLFSIGYLIGAFFFLWQGTDFYLHSIEYILKRWNILICYNVFVIGAKAFLQLFGCLFLEQLVNNNCWVMQLFGMNCLSSALPPLPPGTVDPGEQCNIPPASDAGLFWDGMCFAFLLLQRRLFSSHYFCHIINESKASKYLASRGAELIEELRIKESAQEAERERQILEKIKAKMDRIKATQQKILEAVQDPATHAIAIRSGDYYMFEDVEQEFELDLMEPHEYDDELDERSQKDQRLRRRLTLGRLAAQLRKQRQQEVYELAYGKRDANEPDDDDDDDDTNAAVDQPLTLASSDLPAEEDPAQPPALRRRRLSSPVVLRRDHPTHTDHRGTDDRTAALERSALSEPLPPIEAQYPPPPPQPLPTLPQTAHSGTRLRKPTALDAPDAQTAGPSKELHAQHSITDEEVRLLSDEEDEVEGRKEADVAEEKKSKFSLSGLALIPAFLAGVVGSVTLRLHRASRNYRYVMRVLTREKQVLKETPGFGVGVRDKDGVWTHYISRSGSKTDGSSTEAVKRTLDEVSSEEDFSSRKHSVLFELFQAAWYAIMSHTDFICYFVVFLNQVNSASLLSLPLPLLVTLWGTLTFPRPAQTFWTMLIAYTQMVVLVKCIGQFEMFWWNQTPIPSNQPFHVARLFGIERKDGYATYDLILLLMLYCHRFILKSLGLWKSDPTEEPALSEGLYQVDPNDERAKALMALAEENEKKRETLEIRPPEELTGLSMVKINEKAGTIVEEPIGEESNVLCVSIHPEPTKKYFPQIAKEAFLRYSAALKAFFVQLLDRQSRKTTDVYGYMFLCDFINFFVILFGFSSFGTQDGDGGVLSYFEENRVPVTFLLMLIIQFFLIVVDRALYLRKNIQGKIIFQFILIIGLHVWMFFVLPATTERNFNATTPPVLYYMIKCFYLLFSAYQIRCGYPARILGNFLTKGFTMANFSGFKLFMTVPFLFELRTLMDWIWTDTSMTLFDWLKMEDIFSNVYQLKCMRQLEEDLPAPRGQKKGAMVKYLMGGGMMLGIIFLIWFPLALFAFSNAVGQPNIPHDVSVELRIGTYEPVYAMSAQNNSIYGLTPNNWSNFTAPFMERSAQTFLSNYEPADVAAVQLGISSASIWNISPPDRNRLLYDLINNVTLTCRFRYTISRMTNSKENPGVISEERTYQLYDGPARQALINSLSRTKPDEMAVLMNIMPKFLRVQNSGSIRPVHQLVKTADGDEDEENYRNMQLRQLYMDETSNTSWWQAMEDCTDTLYEKYFSRLPYADCTNFLVIYMFNDKIFPSTISSIAAGGIIGIYSTMILVFSRMLRTSIFSGASSKIMFEDLPYVDRVLQLCLDIYLVRESSEFTLEEDLFAKLLFLYRSPETMIKWTRPKNEGGGDDETDTMTDQPETHPKQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VXI0</accession>
    <name>A0A6E8VXI0_ANOCL</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
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            <taxon>Metazoa</taxon>
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            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RGS7BP/RGS9BP family.</text>
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        <property type="protein sequence ID" value="ACON009306-PB"/>
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        <property type="component" value="Unplaced"/>
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        <property type="term" value="P:negative regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026512">
        <property type="entry name" value="RGS7BP/RGS9BP"/>
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    <dbReference type="PANTHER" id="PTHR21029">
        <property type="entry name" value="R-SEVEN BINDING PROTEIN (R7BP) HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0734">Signal transduction inhibitor</keyword>
    <evidence type="ECO:0000256" key="1">
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    <evidence type="ECO:0000256" key="2">
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    <evidence type="ECO:0000313" key="3">
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
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    <sequence length="145" mass="16629" checksum="F3A9A52F30B6BFB0" modified="2020-08-12" version="1">AIAEGILTDNPHLVLLFYLAQLFLRELIKSHRLVQVIPMDMSGYYGKFNRAGPSNLGNVISQILLCKQITPDFNQEELCSITKDSQEIARILQELQEFMPQQENYLERTIALTTEINGPWPAKRRRGSLYKNMGLLCCVSRPNYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VXJ1</accession>
    <name>A0A6E8VXJ1_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Dynein intermediate chain 2, ciliary</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
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    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
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                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynein intermediate chain family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VXJ1"/>
    <dbReference type="EnsemblMetazoa" id="ACON008689-RB">
        <property type="protein sequence ID" value="ACON008689-PB"/>
        <property type="gene ID" value="ACON008689"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009747"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008689"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030305"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036157">
        <property type="term" value="C:outer dynein arm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045504">
        <property type="term" value="F:dynein heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045503">
        <property type="term" value="F:dynein light chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003341">
        <property type="term" value="P:cilium movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036158">
        <property type="term" value="P:outer dynein arm assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000611">
        <property type="entry name" value="Dynein axonemal intermediate chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050687">
        <property type="entry name" value="Dynein_IC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442:SF11">
        <property type="entry name" value="DYNEIN AXONEMAL INTERMEDIATE CHAIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442">
        <property type="entry name" value="DYNEIN INTERMEDIATE CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0966">Cell projection</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0243">Dynein</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8" id="KW-0505">Motor protein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="101"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="199"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="101"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="118"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="200"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008689-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="671" mass="76714" checksum="19BE77FF5C32A0EE" modified="2020-08-12" version="1">MPLKRRNTNRISSMQKIGKGEPVDNDEFDTWMKSKQLLKPDDQLDLTEAELGEEIPKLLSTENRHLPRNLVVYDFREGAFLPVPPPENTITLLEFEGTSIHKDTDEAKEQISRKGTDDEAEGEGEGQEQEEEVQPPPEEEAPKKKLTNQFNFCERAALTIANPSRSVDTQTIPPPRSSYGASVLQWVIYDSYIEDYAQQQREKEREKEKKPGLQRRDEKSRKDEKAKQAEEFNKRYLQACQIIERMVNQNIYDEIAQDYRYWEDPSDEFREEEGTLLPLWKFSYERTKKMCVTDLCFNTLYYDLFAVCFGTLDFMKQNAEGAVCLFTIKNPSFPDYRIATESGAMCCDIHPKYPYLIAIGLYDGNVIVYNLQVGTKEGPVYISHGVNGKHAECVWEIKWGPDMQDGEINFFSVSADGKVFNWVLMQNKLAITTIITLFLELDQVSGPDGSTLRLKGSGTCMVFHPHNQEIFLVGTEEGYIFKCSTAYSSKYLMTYHAHYLPVHRMDYNKFNSNIFASCSGDWRVKIWEDMRPEPLFIFDLGSSVGDVKWAPYSSTVFAAVTTEGKVFVFDLNVNKYKAICTQAIVSKRKNKLTRLAFNQKLPFIIVGDDKGTTITLKLSPNLRIKTKMGKKAGVVDPQLLESQKLDRLLSLVRELPEGETVKETVSTVASN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VXJ3</accession>
    <name>A0A6E8VXJ3_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Glucosamine-6-phosphate isomerase</fullName>
            <ecNumber evidence="10">3.5.99.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Glucosamine-6-phosphate isomerase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the reversible conversion of alpha-D-glucosamine 6-phosphate (GlcN-6P) into beta-D-fructose 6-phosphate (Fru-6P) and ammonium ion, a regulatory reaction step in de novo uridine diphosphate-N-acetyl-alpha-D-glucosamine (UDP-GlcNAc) biosynthesis via hexosamine pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>alpha-D-glucosamine 6-phosphate + H2O = beta-D-fructose 6-phosphate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:12172"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:75989"/>
            <dbReference type="EC" id="3.5.99.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; alpha-D-glucosamine 6-phosphate from D-fructose 6-phosphate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 10">Homohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">Belongs to the glucosamine/galactosamine-6-phosphate isomerase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.99.6" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8VXJ3"/>
    <dbReference type="SMR" id="A0A6E8VXJ3"/>
    <dbReference type="EnsemblMetazoa" id="ACON009305-RA">
        <property type="protein sequence ID" value="ACON009305-PA"/>
        <property type="gene ID" value="ACON009305"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_008669"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON009305"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037824"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004342">
        <property type="term" value="F:glucosamine-6-phosphate deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006043">
        <property type="term" value="P:glucosamine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006046">
        <property type="term" value="P:N-acetylglucosamine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019262">
        <property type="term" value="P:N-acetylneuraminate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01399">
        <property type="entry name" value="GlcN6P_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1360:FF:000004">
        <property type="entry name" value="Glucosamine-6-phosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01241">
        <property type="entry name" value="GlcN6P_deamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006148">
        <property type="entry name" value="Glc/Gal-6P_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004547">
        <property type="entry name" value="Glucosamine6P_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018321">
        <property type="entry name" value="Glucosamine6P_isomerase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00502">
        <property type="entry name" value="nagB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11280">
        <property type="entry name" value="GLUCOSAMINE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11280:SF5">
        <property type="entry name" value="GLUCOSAMINE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01182">
        <property type="entry name" value="Glucosamine_iso"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01161">
        <property type="entry name" value="GLC_GALNAC_ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="6 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucosamine/galactosamine-6-phosphate isomerase" evidence="11">
        <location>
            <begin position="17"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009305-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="273" mass="31023" checksum="48AAE28FEEF1CF41" modified="2020-08-12" version="1">MRLIILDTAEYVGEWSAKYVMKRINDFKPGPDRYFTLGLPTGSTPLGMYRHLIKFHQQGRISFKYVKTFNMDEYVDLPRDHPESYHYFMWHNFFKHIDIDPENVHILDGNAPDLVAECDAFEEKIRAAGGIELFIGGIGPDGHIAFNEPGSSLASRTRVKTLAQDTLEANARFFGNDISKVPKQALTVGVATVMDAREVMIMILGSHKAFALYKAIEEGVNHMWTVSAFQQHPHTIMICDEDATLELRVKTVKYFKDCYKLSADGLEQATLRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VXJ4</accession>
    <name>A0A6E8VXJ4_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Apple domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VXJ4"/>
    <dbReference type="EnsemblMetazoa" id="ACON009003-RB">
        <property type="protein sequence ID" value="ACON009003-PB"/>
        <property type="gene ID" value="ACON009003"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012348"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON009003"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_029486"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01099">
        <property type="entry name" value="PAN_AP_HGF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.4.10">
        <property type="entry name" value="Hepatocyte Growth Factor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052774">
        <property type="entry name" value="Celegans_DevNeuronal_Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47327:SF13">
        <property type="entry name" value="APPLE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47327">
        <property type="entry name" value="FI18240P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00473">
        <property type="entry name" value="PAN_AP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57414">
        <property type="entry name" value="Hairpin loop containing domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50948">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Apple" evidence="2">
        <location>
            <begin position="36"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="2">
        <location>
            <begin position="430"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="2">
        <location>
            <begin position="533"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="201"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="214"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="262"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="294"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009003-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="908" mass="104633" checksum="7663308557B3F042" modified="2020-08-12" version="1">MVFDVSKALIICLTISGLIPEYITSTIIIVEDAKACYRRVLAGKRVAPHWVRRTLICERVEDCQRECGDERRFSCEGFNYRLDPTGRGQGDCELIDQPLSQIDLYSSPHQRDSNLIRDADYDYYERDRSASSNCRPSCKDCMLKPFRPTLEFVRPTTYRPHHPESYKPYPSEHHAYEEDHRYKPSVITALDKYRPPMYPSRPEFERYSPSSSYYPPPPPLPPPASAPLDSYRPAPPPYKPPSYGGSDIDRYGTLDHGFFKPYETRPDYPRPLHHEDIRPAHRPRHPYEDRDPPAGHPAPPGPPSHVATVQQYGSKPSTFIPYLIGQNIHKNGGIYGGSYGPSFNVDSYKSISDYWGLRNEIKRYDSPSFNYFELRNDHHFDDNNVWSYGGSKYGYEEDHHLLQHPYAVEHHRPSGFGPQQWIRRPSHEECSVKSSEGFRLHKGVVKYALNTPTVIECERMCYSETRFRCHTFSYRYSAVSRENCLLCDRPFNLLDFYADLEPDRDYDIYSMSDDAKTCHPELNHPRRDYNAQCFIRVIDSARFFKSIVRDSLTVRSIGECELECIKAAKFTCRAFTYSFGPNAVNAVIDNCQLSDWPVRDMDKDRHLVPDESFDVFERASYGQGCEIQPIIDDKHSKKFCYLGYGSPAKLLSSAIKKVTSVNTELDCKNECVRLREGTHFKCLSFSFSAQASTYNCEMSDLDQSELKLGVHYAHTNDRDFWLFAWNPFDYTCRDKITTISGGTRANHDRRIDVLREPGDGSRRQYTVSGKPCRLGTKCERNKITGFYSCEIEGGEIGSWDYCCKTEHPCGYSHGFDYPWCFVGDAPDQWRKCSDKYFPKKQHDTRYQDANKKGEIYQPPPKPGGLRHLDGDGVTAPAELWPVTYLYEKGPPNATEISNHVIDCKKDKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VZK5</accession>
    <name>A0A6E8VZK5_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Myosin heavy chain</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Muscle contraction.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Myofibril</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040234085.1">
        <property type="nucleotide sequence ID" value="XM_040378151.2"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON010147-RH">
        <property type="protein sequence ID" value="ACON010147-PH"/>
        <property type="gene ID" value="ACON010147"/>
    </dbReference>
    <dbReference type="GeneID" id="120956570"/>
    <dbReference type="CTD" id="35007"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009622"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON010147"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041963"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031672">
        <property type="term" value="C:A band"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032982">
        <property type="term" value="C:myosin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008307">
        <property type="term" value="F:structural constituent of muscle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007298">
        <property type="term" value="P:border follicle cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040011">
        <property type="term" value="P:locomotion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006936">
        <property type="term" value="P:muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031033">
        <property type="term" value="P:myosin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007424">
        <property type="term" value="P:open tracheal system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045214">
        <property type="term" value="P:sarcomere organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14909">
        <property type="entry name" value="MYSc_Myh1_insects_crustaceans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.820:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000036">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000008">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.530:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.360:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.4820:FF:000002">
        <property type="entry name" value="Myosin heavy chain 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000038">
        <property type="entry name" value="Myosin heavy chain muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000019">
        <property type="entry name" value="Myosin heavy chain, isoform G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000021">
        <property type="entry name" value="Myosin heavy chain, isoform G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000025">
        <property type="entry name" value="Myosin heavy chain, isoform G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000009">
        <property type="entry name" value="Myosin heavy chain, isoform G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000010">
        <property type="entry name" value="Myosin heavy chain, isoform G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000005">
        <property type="entry name" value="Myosin heavy chain, isoform I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000024">
        <property type="entry name" value="Myosin heavy chain, isoform J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.720:FF:000001">
        <property type="entry name" value="Myosin heavy chain, muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000050">
        <property type="entry name" value="Myosin heavy chain, muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.370">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.360">
        <property type="entry name" value="Myosin S1 fragment, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008989">
        <property type="entry name" value="Myosin_S1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002928">
        <property type="entry name" value="Myosin_tail"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004009">
        <property type="entry name" value="SH3_Myosin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014751">
        <property type="entry name" value="XRCC4-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615:SF27">
        <property type="entry name" value="MYOSIN HEAVY CHAIN, MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615">
        <property type="entry name" value="MYOSIN HEAVY CHAIN, NON-MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02736">
        <property type="entry name" value="Myosin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01576">
        <property type="entry name" value="Myosin_tail_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90257">
        <property type="entry name" value="Myosin rod fragments"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51844">
        <property type="entry name" value="SH3_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 14" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="6 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 14" id="KW-0505">Motor protein</keyword>
    <keyword evidence="10" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="8 14" id="KW-0518">Myosin</keyword>
    <keyword evidence="5 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0787">Thick filament</keyword>
    <feature type="domain" description="Myosin N-terminal SH3-like" evidence="17">
        <location>
            <begin position="34"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="Myosin motor" evidence="16">
        <location>
            <begin position="87"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="14">
        <location>
            <begin position="657"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1823"/>
            <end position="1842"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1920"/>
            <end position="1961"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="180"/>
            <end position="187"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010147-PH"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="20">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1961" mass="223758" checksum="8298F60CE77D7311" modified="2020-08-12" version="1">MPKPPVQVGEDPDPTEFLFVSLEQKRIDQSKPYDSKKACWVPEEKEGYVLGEIKATKGELVTVALPGGEEKNFKKEQLSQVNPPKFEKVEDMADLTYLNEAAVLHNLRQRYYSKLIYTYSGLFCVVINPYKRYPLYTNRCAKMYRGKRRNEVPPHLFAVSDGAYVNMLTNHENQSMLITGESGAGKTENTKKVIAYFATIGASGKKDENAEKKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFTGSGKLAGADIETYLLEKARVISQQTLERSYHIFYQIMSGSVKGLKEKCLLSNNIHDYHIVAQGKTTIPSVDDGEEMQITDEAFNVLGFTQEEKDNIYRITSAVMHMGRMQFKQKGREEQAEADGTEDGDRVAKLLGVGTDDLYKNLLKPRIKVGNEFVTKGQNKDQVTNSVGALCKGIFDRLFKWLVKKCNETLDTKQKRAQFIGVLDIAGFEIFDFNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGINWAFIDFGMDLLACVELIEKPMGILSILEEESMFPKATDQTFAEKLMTNHLGKSAPFMKPRPPKPGIPAGHFAIGHYAGVVSYNITGWLEKNKDPLNDTVVDQFKKGSNALMVEIFADHPGQSADPAAAKGGRGKKGAGFATVSSSYKEQLNNLMTTLKSTQPHFVRCIIPNEMKTAGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKLRYLILAPAAMQAETEGKKAAEKCFEAIGLDPDSYRIGHTKVFFRAGVLGQMEEFRDERLSKIMSWMQAWCRGYLSRKEFKKMQEQRVSLEIVQRNLRKYLKLRTWAWWKLWQKVKPLLNVSRVEDQIAKLEEKATKAQEAYEKEEKLRKELEALNSKLLAEKTALLDSLSGEKGALQEYQEKAAKLTAQKNDLENQLRDTQERLAQEEDARNQLFQTKKKLEQEIGSQKKDAEDLELQIQKIEQDKASKDHQIRNLNDEIAHQDELINKLNKEKKMQGEVNQKTAEELQAAEDKVNHLNKVKAKLEQTLDELEDSLEREKKLRGDVEKAKRKVEGDLKLTQEAVADLERNKKELEQTVLRKDKEISALSAKLEDEQSLVGKLQKQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELAKLRRDLEEANIQHEGTLANLRKKHNDAVAEMAEQVDQLNKLKTKAEKERTQYFAELNDARIGCDQLSNEKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEDAESQVSQLSKIKISLTQQLEDTKRLADEEARERATLLGKFRNLEHDLDNLREQVEEEAEGKGDIQRQLSKANAEAQLWRSKYESEGVARAEELEEAKRKLQARLAEAEETIESLNQKCIALEKTKQRLATEVEDLQLEVDRASSIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKSRKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDVQSALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELSDAHEQLNEVSAQNASIAAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAESNALKGGKKAIQKLEQRVRELESELDSEQRRHADAQKNLRKSERRIKELTFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADIAEQAATKFRTKGGRAGSVQRGASPAPQRQPSAMPALAGLNLPTFDDHGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VZM4</accession>
    <name>A0A6E8VZM4_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Allatostatin c</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VZM4"/>
    <dbReference type="EnsemblMetazoa" id="ACON010157-RB">
        <property type="protein sequence ID" value="ACON010157-PB"/>
        <property type="gene ID" value="ACON010157"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_013078"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON010157"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_036763"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010157-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="83" mass="9610" checksum="3AEA2F3D21DDEA97" modified="2020-08-12" version="1">DLPEINPNELRKLYTNYNSYVSNQLDNYGLDPLQLQLLAQYAQSNAISGGGGGGWDQLYRAPEMKRQIRYRQCYFNPISCFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VZN1</accession>
    <name>A0A6E8VZN1_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Myosin heavy chain</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Muscle contraction.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Myofibril</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON010147-RE">
        <property type="protein sequence ID" value="ACON010147-PE"/>
        <property type="gene ID" value="ACON010147"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009622"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON010147"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041963"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031672">
        <property type="term" value="C:A band"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032982">
        <property type="term" value="C:myosin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008307">
        <property type="term" value="F:structural constituent of muscle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007298">
        <property type="term" value="P:border follicle cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040011">
        <property type="term" value="P:locomotion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006936">
        <property type="term" value="P:muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031033">
        <property type="term" value="P:myosin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007424">
        <property type="term" value="P:open tracheal system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045214">
        <property type="term" value="P:sarcomere organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14909">
        <property type="entry name" value="MYSc_Myh1_insects_crustaceans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.820:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000036">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000008">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.530:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.360:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.4820:FF:000002">
        <property type="entry name" value="Myosin heavy chain 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000038">
        <property type="entry name" value="Myosin heavy chain muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000019">
        <property type="entry name" value="Myosin heavy chain, isoform G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000021">
        <property type="entry name" value="Myosin heavy chain, isoform G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000025">
        <property type="entry name" value="Myosin heavy chain, isoform G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000009">
        <property type="entry name" value="Myosin heavy chain, isoform G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000010">
        <property type="entry name" value="Myosin heavy chain, isoform G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000005">
        <property type="entry name" value="Myosin heavy chain, isoform I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000024">
        <property type="entry name" value="Myosin heavy chain, isoform J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.720:FF:000001">
        <property type="entry name" value="Myosin heavy chain, muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000050">
        <property type="entry name" value="Myosin heavy chain, muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.370">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.360">
        <property type="entry name" value="Myosin S1 fragment, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008989">
        <property type="entry name" value="Myosin_S1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002928">
        <property type="entry name" value="Myosin_tail"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004009">
        <property type="entry name" value="SH3_Myosin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014751">
        <property type="entry name" value="XRCC4-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615:SF27">
        <property type="entry name" value="MYOSIN HEAVY CHAIN, MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615">
        <property type="entry name" value="MYOSIN HEAVY CHAIN, NON-MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02736">
        <property type="entry name" value="Myosin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01576">
        <property type="entry name" value="Myosin_tail_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90257">
        <property type="entry name" value="Myosin rod fragments"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51844">
        <property type="entry name" value="SH3_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 14" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="6 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 14" id="KW-0505">Motor protein</keyword>
    <keyword evidence="10" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="8 14" id="KW-0518">Myosin</keyword>
    <keyword evidence="5 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0787">Thick filament</keyword>
    <feature type="domain" description="Myosin N-terminal SH3-like" evidence="17">
        <location>
            <begin position="34"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="Myosin motor" evidence="16">
        <location>
            <begin position="87"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="14">
        <location>
            <begin position="657"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1823"/>
            <end position="1842"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1920"/>
            <end position="1961"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="180"/>
            <end position="187"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010147-PE"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="20">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1961" mass="224139" checksum="73E8E9182D02F096" modified="2020-08-12" version="1">MPKPPVQVGEDPDPTEFLFVSLEQKRIDQSKPYDSKKACWVPEEKEGYVLGEIKATKGELVTVALPGGEEKNFKKEQLSQVNPPKFEKVEDMADLTYLNEAAVLHNLRQRYYSKLIYTYSGLFCVVINPYKRYPLYTNRCAKMYRGKRRNEVPPHLFAVSDGAYVNMLTNHENQSMLITGESGAGKTENTKKVIAYFATIGASGKKDENAEKKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFTGSGKLAGADIETYLLEKARVISQQTLERSYHIFYQIMSGSVKGLKEKCLLSNNIHDYHIVAQGKTTIPSVDDGEEMQITDEAFNVLGFTQEEKDNIYRITSAVMHMGRMQFKQKGREEQAEADGTEDGDRVAKLLGVGTDDLYKNLLKPRIKVGNEFVTKGQNKDQVTNSVGALCKGIFDRLFKWLVKKCNETLDTKQKRAQFIGVLDIAGFEIFDFNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGINWAFIDFGMDLLACVELIEKPMGILSILEEESMFPKATDQTFAEKLMTNHLGKSAPFMKPRPPKPGIPAGHFAIGHYAGVVSYNITGWLEKNKDPLNDTVVDQFKKGSNALMVEIFADHPGQSADPAAAKGGRGKKGAGFATVSSSYKEQLNNLMTTLKSTQPHFVRCIIPNEMKTAGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKLRYKILCPQLIKEPCSPEKVTQIVLTHIQLPEEQFRMGKTKVFFRAGVLGQMEEFRDERLSKIMSWMQAWCRGYLSRKEFKKMQEQRVSLEIVQRNLRKYLKLRTWAWWKLWQKVKPLLNVSRVEDQIAKLEEKATKAQEAYEKEEKLRKELEALNSKLLAEKTALLDSLSGEKGALQEYQEKAAKLTAQKNDLENQLRDTQERLAQEEDARNQLFQTKKKLEQEIGSQKKDAEDLELQIQKIEQDKASKDHQIRNLNDEIAHQDELINKLNKEKKMQGEVNQKTAEELQAAEDKVNHLNKVKAKLEQTLDELEDSLEREKKLRGDVEKAKRKVEGDLKLTQEAVADLERNKKELEQTVLRKDKEISALSAKLEDEQSLVGKLQKQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELAKLRRDLEEANIQHEGTLANLRKKHNDAVAEMAEQVDQLNKLKTKAEHDRANMYNELNNTRTACDQLSREKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEDAESQVSQLSKIKISLTQQLEDTKRLADEEARERATLLGKFRNLEHDLDNLREQVEEEAEGKGDIQRQLSKANAEAQLWRSKYESEGVARAEELEEAKRKLQARLAEAEETIESLNQKCIALEKTKQRLATEVEDLQLEVDRASSIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKSRKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDVQSALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELSDAHEQLNEVSAQNASIAAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAESNALKGGKKAIQKLEQRVRELESELDSEQRRHADAQKNLRKSERRIKELTFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADIAEQAATKFRTKGGRAGSVQRGASPAPQRQPSAMPALAGLNLPTFDDHGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VZN7</accession>
    <name>A0A6E8VZN7_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Myosin heavy chain</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Muscle contraction.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Myofibril</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON010147-RJ">
        <property type="protein sequence ID" value="ACON010147-PJ"/>
        <property type="gene ID" value="ACON010147"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009622"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON010147"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041963"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031672">
        <property type="term" value="C:A band"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032982">
        <property type="term" value="C:myosin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008307">
        <property type="term" value="F:structural constituent of muscle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007298">
        <property type="term" value="P:border follicle cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040011">
        <property type="term" value="P:locomotion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006936">
        <property type="term" value="P:muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031033">
        <property type="term" value="P:myosin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007424">
        <property type="term" value="P:open tracheal system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045214">
        <property type="term" value="P:sarcomere organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14909">
        <property type="entry name" value="MYSc_Myh1_insects_crustaceans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.820:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000036">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000008">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.530:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.360:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.4820:FF:000002">
        <property type="entry name" value="Myosin heavy chain 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000038">
        <property type="entry name" value="Myosin heavy chain muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000019">
        <property type="entry name" value="Myosin heavy chain, isoform G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000021">
        <property type="entry name" value="Myosin heavy chain, isoform G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000025">
        <property type="entry name" value="Myosin heavy chain, isoform G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000009">
        <property type="entry name" value="Myosin heavy chain, isoform G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000010">
        <property type="entry name" value="Myosin heavy chain, isoform G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000005">
        <property type="entry name" value="Myosin heavy chain, isoform I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000024">
        <property type="entry name" value="Myosin heavy chain, isoform J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.720:FF:000001">
        <property type="entry name" value="Myosin heavy chain, muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000050">
        <property type="entry name" value="Myosin heavy chain, muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.370">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.360">
        <property type="entry name" value="Myosin S1 fragment, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008989">
        <property type="entry name" value="Myosin_S1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002928">
        <property type="entry name" value="Myosin_tail"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004009">
        <property type="entry name" value="SH3_Myosin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014751">
        <property type="entry name" value="XRCC4-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615:SF27">
        <property type="entry name" value="MYOSIN HEAVY CHAIN, MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615">
        <property type="entry name" value="MYOSIN HEAVY CHAIN, NON-MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02736">
        <property type="entry name" value="Myosin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01576">
        <property type="entry name" value="Myosin_tail_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90257">
        <property type="entry name" value="Myosin rod fragments"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51844">
        <property type="entry name" value="SH3_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 14" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="6 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 14" id="KW-0505">Motor protein</keyword>
    <keyword evidence="10" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="8 14" id="KW-0518">Myosin</keyword>
    <keyword evidence="5 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0787">Thick filament</keyword>
    <feature type="domain" description="Myosin N-terminal SH3-like" evidence="17">
        <location>
            <begin position="34"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="Myosin motor" evidence="16">
        <location>
            <begin position="87"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="14">
        <location>
            <begin position="657"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1823"/>
            <end position="1842"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1920"/>
            <end position="1961"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="180"/>
            <end position="187"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010147-PJ"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="20">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1961" mass="224090" checksum="82B3D06B718A35E8" modified="2020-08-12" version="1">MPKPPVQVGEDPDPTEFLFVSLEQKRIDQSKPYDSKKACWVPEEKEGYVLGEIKATKGELVTVALPGGETKDFKKDLVSQVNPPKYEKCEDMSNLTYLNDASVLHNLRQRYYAKLIYTYSGLFCVVINPYKRYPLYTNRCAKMYRGKRRNEVPPHLFAVSDGAYVNMLTNHENQSMLITGESGAGKTENTKKVIAYFATIGASGKKDENAEKKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFTGSGKLAGADIETYLLEKARVISQQTLERSYHIFYQIMSGSVKGLKEKCLLSNNIHDYHIVAQGKTTIPSVDDGEEMQITDEAFNVLGFTQEEKDNIYRITSAVMHMGRMQFKQKGREEQAEADGTEDGDRVAKLLGVGTDDLYKNLLKPRIKVGNEFVTKGQNKDQVTNSVGALCKGIFDRLFKWLVKKCNETLDTKQKRAQFIGVLDIAGFEIFDFNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGINWAFIDFGMDLLACVELIEKPMGILSILEEESMFPKATDQTFAEKLMTNHLGKSAPFMKPRPPKPGIPAGHFAIGHYAGVVSYNITGWLEKNKDPLNDTVVDQFKKGSNALMVEIFADHPGQSADPAAAKGGRGKKGAGFATVSSSYKEQLNNLMTTLKSTQPHFVRCIIPNEMKTAGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKLRYKILCPQLIKEPCSPEKVTQIVLTHIQLPEEQFRMGKTKVFFRAGVLGQMEEFRDERLSKIMSWMQAWCRGYLSRKEFKKMQEQRVSLEIVQRNLRKYLKLRTWAWWKLWQKVKPLLNVSRVEDQIAKLEEKATKAQEAYEKEEKLRKELEALNSKLLAEKTALLDSLSGEKGALQEYQEKAAKLTAQKNDLENQLRDTQERLAQEEDARNQLFQTKKKLEQEIGSQKKDAEDLELQIQKIEQDKASKDHQIRNLNDEIAHQDELINKLNKEKKMQGEVNQKTAEELQAAEDKVNHLNKVKAKLEQTLDELEDSLEREKKLRGDVEKAKRKVEGDLKLTQEAVADLERNKKELEQTVLRKDKEISALSAKLEDEQSLVGKLQKQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELAKLRRDLEEANIQHEGTLANLRKKHNDAVAEMAEQVDQLNKLKTKAEHDRANMYNELNNTRTACDQLSREKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEDAESQVSQLSKIKISLTQQLEDTKRLADEEARERATLLGKFRNLEHDLDNLREQVEEEAEGKGDIQRQLSKANAEAQLWRSKYESEGVARAEELEEAKRKLQARLAEAEETIESLNQKCIALEKTKQRLATEVEDLQLEVDRASSIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKSRKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDVQSALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELSDAHEQLNEVSAQNASIAAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAESNALKGGKKAIQKLEQRVRELESELDSEQRRHADAQKNLRKSERRIKELTFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADIAEQAATKFRTKGGRAGSVQRGASPAPQRQPSAMPALAGLNLPTFDDHGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W0X0</accession>
    <name>A0A6E8W0X0_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ATPase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AFG1 ATPase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8W0X0"/>
    <dbReference type="EnsemblMetazoa" id="ACON009867-RA">
        <property type="protein sequence ID" value="ACON009867-PA"/>
        <property type="gene ID" value="ACON009867"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002846"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON009867"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035684"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005654">
        <property type="entry name" value="ATPase_AFG1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040713">
        <property type="entry name" value="ZapE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12169:SF6">
        <property type="entry name" value="AFG1-LIKE ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12169">
        <property type="entry name" value="ATPASE N2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03969">
        <property type="entry name" value="AFG1_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009867-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="331" mass="38046" checksum="950340AC1C7FF930" modified="2020-08-12" version="1">IDRKRRVHFNSFMTDVHAKIHDIKSKHVRDASNSKPQPFDPIKPVAELITQDSWMICFDEFQVTDIADAMILKRLFTYLFNNGVIVVATSNRAPDDLYKNGLQRSNFVPFIGVLKNHCNIVTLDSGVDYRTAALKGESKHYFDKSQGASDANASMDKLFKVLCSQENDMIRPKTFTHFGRNITFAKTCGQVLDSTFDELCDKPLGASDFLQIAQFFHTVLIRDIPQLNLKLKSQTRRFITLIDTLYDSRVRLVVSADVPYKYLFSNDAPDDMHTSDEHRMLMDDLKITKDSTDASSNIFTGEEEVFAFERTVSRLAEMQSAEYWTLWEKHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W0Y0</accession>
    <name>A0A6E8W0Y0_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Gustatory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Gustatory receptor which mediates acceptance or avoidance behavior, depending on its substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the insect chemoreceptor superfamily. Gustatory receptor (GR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8W0Y0"/>
    <dbReference type="EnsemblMetazoa" id="ACON009805-RB">
        <property type="protein sequence ID" value="ACON009805-PB"/>
        <property type="gene ID" value="ACON009805"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACON009805"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034541"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007635">
        <property type="term" value="P:chemosensory behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008049">
        <property type="term" value="P:male courtship behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050909">
        <property type="term" value="P:sensory perception of taste"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013604">
        <property type="entry name" value="7TM_chemorcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21143:SF134">
        <property type="entry name" value="GUSTATORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21143">
        <property type="entry name" value="INVERTEBRATE GUSTATORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08395">
        <property type="entry name" value="7tm_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="39"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="70"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="135"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="164"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="232"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="352"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009805-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="395" mass="45982" checksum="7B024917966326BB" modified="2020-08-12" version="1">MSACARLNRYTFKIMSSVYYLPVAYDAVEHRFIEKRSNLISFAIGLLLSIGFIYHDFFIVLANYNTDQSAFTLAVFIVELTVFATVPICAVCNSFVHRKEIVQLLNMLFADQNVLDLGGTYGNKSVQPFSIANRYVKVLYAIVVLICFYNYINMIHTMHTFLELTLISRFLLTTQYIYLYYLCVSMVRLRMQQLRVLFLDHQHEHDFEQLLCLFLERFRLYIAQIERINQCLSAPLLGMLLQALIELAYFMYEWFRVISTGQVVNGNYISVQQWITSQFWQLMYGNVLLLLVPSCEQASNEVEETALCTRHFDDYRLQNTRAAKQIQKFLLKNLHQKKKFSACGFFDIDNTVIYMVFSSIVTYLVILIQFKQLETDLTQAGDGYNVTSNVSTVQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W0Y9</accession>
    <name>A0A6E8W0Y9_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Gustatory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Gustatory receptor which mediates acceptance or avoidance behavior, depending on its substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the insect chemoreceptor superfamily. Gustatory receptor (GR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8W0Y9"/>
    <dbReference type="EnsemblMetazoa" id="ACON009805-RL">
        <property type="protein sequence ID" value="ACON009805-PL"/>
        <property type="gene ID" value="ACON009805"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACON009805"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007635">
        <property type="term" value="P:chemosensory behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008049">
        <property type="term" value="P:male courtship behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050909">
        <property type="term" value="P:sensory perception of taste"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013604">
        <property type="entry name" value="7TM_chemorcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21143:SF133">
        <property type="entry name" value="GUSTATORY AND PHEROMONE RECEPTOR 32A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21143">
        <property type="entry name" value="INVERTEBRATE GUSTATORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08395">
        <property type="entry name" value="7tm_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="79"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="136"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="179"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="247"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="362"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009805-PL"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="405" mass="46355" checksum="7DAC762A52CB7689" modified="2020-08-12" version="1">MHVFDNIHVLLRWLRFLGLISVQKCQCSDHRSGSNPFKSTNNAAPKFMILFALSLLTSGHSIFRGTIDNSVIDALDQGTIWPFVYLVHQITLCVALCAFPWQTFWQRKRLSAAMNILAQNEINLIARTGVRTDYRIVSWLAIAIVCNGMCLHLFFQVFYIVQYGGMDKPFLPLYITSCAFMYVDLAMELVLGLCDCLLLIVRLQLQRLVCSARNLERSGNQEDDFLTFYGTMYCKIAVVLSDHLGPYFGLIILIHCSYVCFEAAICILDMYDLLLNNNSSGLLYLVYIIWPLSDIKKVMILFMLSEQVNRLVEETALCTRHFDDYRLQNTRAAKQIQKFLLKNLHQKKKFSACGFFDIDNTVIYMVFSSIVTYLVILIQFKQLETDLTQAGDGYNVTSNVSTVQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W156</accession>
    <name>A0A6E8W156_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Tyrosine-protein kinase</fullName>
            <ecNumber evidence="10">2.7.10.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 10">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the protein kinase superfamily. Tyr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.2" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8W156"/>
    <dbReference type="EnsemblMetazoa" id="ACON010710-RB">
        <property type="protein sequence ID" value="ACON010710-PB"/>
        <property type="gene ID" value="ACON010710"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012254"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON010710"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040924"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004715">
        <property type="term" value="F:non-membrane spanning protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002009">
        <property type="term" value="P:morphogenesis of an epithelium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd10347">
        <property type="entry name" value="SH2_Nterm_shark_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000027">
        <property type="entry name" value="Receptor protein-tyrosine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050198">
        <property type="entry name" value="Non-receptor_tyrosine_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035061">
        <property type="entry name" value="Shark-like_SH2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24418">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="2 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 10" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="SH2" evidence="12">
        <location>
            <begin position="9"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="8">
        <location>
            <begin position="148"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="8">
        <location>
            <begin position="181"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="8">
        <location>
            <begin position="216"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="12">
        <location>
            <begin position="284"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="848"/>
            <end position="1127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="311"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="432"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="504"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="318"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="446"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="469"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="506"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="536"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="580"/>
            <end position="589"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00051245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010710-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1190" mass="131288" checksum="2CDA4404A54E3A00" modified="2020-08-12" version="1">MNREENLCWFHGKISREDAEEILRREGSDGVFLVRESSSSEGDYVLSVLFKGEVVHYAIRRHGDDAFFSIHDHTPIHGLDSLIEHYQKDKGNLVTRLQVICRSDPPPHDVRSHGTTNLLHRATKESNYTVVSELLKCGYRNIDSKNQDGQTAVHLACLHADDKILLKLIERGANINSRDAKGNTPLHYACAKRNGLEMVRMLIKAAASLQARNSETGWVPLHEAADNGNIEAIQELLSHRVPHRPRTNFGEMPSDLARLRGHYQVVEFLNAYEPQRPLTHRDLWYHGTLERTAAVDYLKDFAVKLLPNLAHRQQQQQPDRDGNKENGECLDGLEQSTSGTYLVRYSAKQNVDVLTMLYDNEPKHFIIQRQQDWLYIDEGPYMNSLEHLIEHYTRFSDGLPINLRFPVTPGPKPPIPAFATIPKSKHRMHRTDGTTAALPSYGGSAHHSTNGSGSLLTAGLTSMFRKSSENPTTATATLPSKGMPSRNLSVPNDAMLQMNRVGLFDRSPERCAPETPRRNSKGPTSPNTPDEPKTKPSGSNASPTLKKTLMDGMKSLRKSKQKLKPAAADPDGGSGTETNSSSASLNVSSIEPTVSPSKLLQQLKFSSDFNLVASCAAADDIYKIPTNNCAIVDIDIGEPPVVAVPALSTSPPVAGLQDDSELPPAIPPKLSAPLLDSVNNNNNNESATLDYFTQSDDPLRSDTLPDDAGEDIYFVEAPIPPPARACLEAAAAAGGASLLNNNNTVTPDDPAAIQPARNAPTNYIMTKTVPIFSSVSVEECQTPTSPTCGGQMRASKLFERLDSNQSSFSRASVLSGESVFFAMFQQQQSSNTAAQPEGPNYFIPKECIHAETVLGRGEFGFVYQGFLDPWPVDIAGGGYQSGGVAHKTSPPVMMGRVPVAIKKVMDSQERRDRTDFLREASVMIRLRHNCIVRLIGICKGPPLIMVQELISLGSMLVYITKNKATINPKQEMMIWAAQIASGMQYLEEKHFVHRDLAARNILLANKYQAKISDFGLSRAIGAGQEYYRASQGGKWPIKWYAPESFNYGTFSHASDVWSFGVTLWEMFSYGAPPYHDMKGAEVIKLIEEDQRLSRPDACPDKVFEVMRNCWQYNPKLRPTFRFLHRFFSDDIEYQNLQELVGTGVEINANATTLTHAGGSSSSNSNNTATITTVEPTVPEVASDSDIGVPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W173</accession>
    <name>A0A6E8W173_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nocturnin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CCR4/nocturin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8W173"/>
    <dbReference type="EnsemblMetazoa" id="ACON009893-RB">
        <property type="protein sequence ID" value="ACON009893-PB"/>
        <property type="gene ID" value="ACON009893"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006742"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON009893"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037958"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004535">
        <property type="term" value="F:poly(A)-specific ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:circadian rhythm"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd09096">
        <property type="entry name" value="Deadenylase_nocturnin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR050410">
        <property type="entry name" value="CCR4/nocturin_mRNA_transcr"/>
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    </dbReference>
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        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNase I-like"/>
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    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
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            <begin position="117"/>
            <end position="434"/>
        </location>
    </feature>
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        <location>
            <begin position="329"/>
            <end position="365"/>
        </location>
    </feature>
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            <dbReference type="ARBA" id="ARBA00010774"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009893-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="463" mass="52375" checksum="0942526407C83E9E" modified="2020-08-12" version="1">MDLLVRTTWVFTTKRFKNLLFIPRMGSFTSAPQIKNVDYQDDKLEITDGMSVPDLVDYCRIARGDERPQLVKRKFLKPIDRLNGSDLTDGFKMLQLDSISKTCSEPSATATQLRIFQWNMLSQTLGMHNDGFVRCPLEALTWECRRYQVIEEIVQNDPDIICLQEVDHFKFLQKILSTQNYEGVFFPKPDSPCLYINGNNGPDGCAVFYKKDRLEMVNHFTRVLEVWRVQSNQVAIAAVLRTLDTQQEFCVTTTHLKARKGALLSKLRNEQGKDLLYFIDGVAENRPVILCGDFNAEPIEPIYSTVLNYKPLGLASAYSDLLAEESQDENNQNALNTGVERSGERCSRSSIGSAHSATDDCGLSNGVRTKAEQSAAYEPPYTTWKIREEGEVCHTIDYVFYSKDKLTVKNCLMFPSGEEIGVDRTPSFQYPSDHFSLVCDIELKPASDGSQSDVCSTIPNHQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W188</accession>
    <name>A0A6E8W188_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8W188"/>
    <dbReference type="EnsemblMetazoa" id="ACON009909-RB">
        <property type="protein sequence ID" value="ACON009909-PB"/>
        <property type="gene ID" value="ACON009909"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_013321"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON009909"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030415"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="12"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009909-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="65" mass="7027" checksum="58C60C5578D4B2A4" modified="2020-08-12" version="1">MQFIRNSLSFSFISNPGSTQQQISPENGPSEAMAPDDGKLPRSASNVSEQTQYATVRSKLPLNLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W191</accession>
    <name>A0A6E8W191_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DUF4739 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8W191"/>
    <dbReference type="EnsemblMetazoa" id="ACON010805-RA">
        <property type="protein sequence ID" value="ACON010805-PA"/>
        <property type="gene ID" value="ACON010805"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON010805-RB">
        <property type="protein sequence ID" value="ACON010805-PB"/>
        <property type="gene ID" value="ACON010805"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011320"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON010805"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032366"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031764">
        <property type="entry name" value="DUF4739"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15893">
        <property type="entry name" value="DUF4739"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4739" evidence="2">
        <location>
            <begin position="471"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="56"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="301"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="386"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="414"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="448"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="496"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="718"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="791"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="852"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="933"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1022"/>
            <end position="1062"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="131"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="186"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="388"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="414"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="465"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="506"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="733"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="852"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="872"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="947"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="965"/>
            <end position="982"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF15893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010805-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1183" mass="128113" checksum="0BF6425E45B0C193" modified="2020-08-12" version="1">MSDYQRSLSEGRLVDSDISRDGLSQSHDSVFSESAGTASSLSITLKNELADVLRKRRKDRQGEVSDEDLGLPLSPITPQRKDRGMNKSEGSLSILSMTSSDLDDDRSASNASGHNLLHLDSSTSGSISMNRSEHMDESDSSKLSHSAAKHKLAVRPKKKGPTRARTRPRESTLLPATPEVNEESLKLSSTNIASSFSPTKETNEKAHSLPYGIVPPGTSTPFAPKSSPAKEISTSKLSVGDAPAVTGSSIYVNRNISKSHEFERSADLHHLTVEGSAVPAASRKEDDGFFKRILNYSFKKKKHETSAKEDSSHSVTASPRKEDNTSSVYISSADPATVDCVPTEQIMKLSLVVGEPELSELSGYKKIKSGPAARQRVFPKDIFTAEEQQLQQQQQQQQHQQHTQRYSSNVEVNRQSVASSGSGHSLTVGEAKMPLHKSEEYLVSKTRKFSERSVDGGEGDDEESDGRKYVSHGERLKSPKVYGLSSYQQKLAKISISSQPAATPDAAKDSPSKRAKSVEKSKSFRTYTTDGVSNQLQAGGHQVPSLPNLSASLSVGNFSSNNFLETEHKFFSMTENMNTGKQRVFKDYISNADDDGESRHLSSSASLQKFEINDNNLLNPREEYYDHQPKSIVLTTNTLTPPEPTSILNKSNQNISQIEENIDKLVSSQFVSIIRSSDEGSSNERLDDIGVGANVPEFMKIQLKNRVEGTGRPAKTSSIVLTTSPTPAPPTSPAIASPTASAGQYPQQPDDAIKLQRRFSNENIEITESKPPLPPSVVGRTSLELGIPKSPPAFRKQGSGGGAAVGDLAGSKRNSMNLSQDLSDDRKVILQRRTSVTEEKIKYERRISSSSEDIKFEKKKSTSEELLEKRSSTGSNGTGNSNYNSGSSSGDELVVLRKKFIGGEREGGKDKDGTPELMKVFARRSLKLRSDDDYKVTESGKPLSSIDSDKENQSSEEKLDKVGLQQTKQQQQQQQQQHVQQQPAEHISTLVQSVAIGAPVTNGSLKNGSSVVEPAVLKNASNENGPAAPASGEPFLRKSITSKPFGVPNRFGNAPNGGQPRNATSFVEVRKTLLPSATVTVGAPVITNNNFVKSSTAQAQGDACESTTVSNNNTINTNRHTIASLNHLNSANGVGAINNNAANTATIIESDGGSGGNNGEINEFKGILQRRAEWEKRAKEGFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W308</accession>
    <name>A0A6E8W308_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">phospholipase A2</fullName>
            <ecNumber evidence="3">3.1.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Phosphatidylcholine 2-acylhydrolase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.1.1.4" evidence="3"/>
    <dbReference type="RefSeq" id="XP_040233246.2">
        <property type="nucleotide sequence ID" value="XM_040377312.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8W308"/>
    <dbReference type="EnsemblMetazoa" id="ACON011569-RB">
        <property type="protein sequence ID" value="ACON011569-PB"/>
        <property type="gene ID" value="ACON011569"/>
    </dbReference>
    <dbReference type="GeneID" id="120955998"/>
    <dbReference type="CTD" id="31747"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_008077"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON011569"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031066"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004623">
        <property type="term" value="F:phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050482">
        <property type="term" value="P:arachidonate secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04704">
        <property type="entry name" value="PLA2_bee_venom_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.90.10">
        <property type="entry name" value="Phospholipase A2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016090">
        <property type="entry name" value="PLA2-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033113">
        <property type="entry name" value="PLA2_histidine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036444">
        <property type="entry name" value="PLipase_A2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12253">
        <property type="entry name" value="RH14732P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05826">
        <property type="entry name" value="Phospholip_A2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48619">
        <property type="entry name" value="Phospholipase A2, PLA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00118">
        <property type="entry name" value="PA2_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Phospholipase A2-like central" evidence="9">
        <location>
            <begin position="320"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="46"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="52"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF05826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON011569-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="445" mass="50941" checksum="79679F946655357A" modified="2020-08-12" version="1">MSKVKVHSSKVQRLESVLKFTVTGLTLLLLTVPVMTVQQEDNSVVTGGPTAHHPHHHTRHQRHQLHRDGTKQQYDDIGDDGGGGGDGGGGGGIIENFLLIDYLNDGERERRLHWNGVVGKETSLADSSSNKTLSLWQMATHKYLMQIIYNSEGQMMDCEYVRQRDMLHQFRDRFFAEFAQARARNFSSAQGGPQTVDRASLVSIHDFRQYLEHDIVTPEMEELQYNVYEFPAGVPAAQQTIDAFGMRNLTYIPLRAVADIPAELLEQLNFPQLKQRCNVRHMQLKEIATGLRSADPEHKRIANEKLQRHKRAISDWFLSPNTKWCGKGHSASEYRQLGGASRADMCCRTHDHCKYMIPPMSTNFQTFNIRPFTISHCACDSRFRTCLKLADSKDANLVGKLFFNVMQMKCFVFKPETVCTKKSWWGTCERKGRRKRAVLRHNRKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W4S4</accession>
    <name>A0A6E8W4S4_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Receptor expression-enhancing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8W4S4"/>
    <dbReference type="EnsemblMetazoa" id="ACON011331-RA">
        <property type="protein sequence ID" value="ACON011331-PA"/>
        <property type="gene ID" value="ACON011331"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000364"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON011331"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042433"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005881">
        <property type="term" value="C:cytoplasmic microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071782">
        <property type="term" value="C:endoplasmic reticulum tubular network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071786">
        <property type="term" value="P:endoplasmic reticulum tubular network organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004345">
        <property type="entry name" value="TB2_DP1_HVA22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12300">
        <property type="entry name" value="HVA22-LIKE PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12300:SF117">
        <property type="entry name" value="LP05237P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03134">
        <property type="entry name" value="TB2_DP1_HVA22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="254"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="299"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU362006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON011331-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="316" mass="35800" checksum="C93F8E7937FD5FB2" modified="2020-08-12" version="1">MLSRIPFPRMSVTGMGLCSLVAIFFCPRAIGSNILFPGFRLLFGTLYPAYASYKAVRTKNVKEYVKWMMYWIVFAFFTFIETFTDILLSWFPFYYEIKVIVVLWLLSPATRGSSTLYRKFVHPMLTRREQEIDDYINQAKEKGYTAVLQLGSKGVNYATNVIMQTAIKGGGGLVQTLRKSYSLSDLSEPDTQRTQDEVDEIVNRPQRVLRSKSSRSSSGSRQMEMYFPEVEIAAAPYHATAAPPYNYIRSSDDISSGYSSAEPGLSRTASMSNTARPRLKSKTREEKMSASCTTLPRTKKPEKVTKTRSKTEKPSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W7C5</accession>
    <name>A0A6E8W7C5_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein TsetseEP domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8W7C5"/>
    <dbReference type="EnsemblMetazoa" id="ACON013311-RB">
        <property type="protein sequence ID" value="ACON013311-PB"/>
        <property type="gene ID" value="ACON013311"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007130"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON013311"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033708"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026288417" evidence="1">
        <location>
            <begin position="22"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON013311-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="390" mass="44205" checksum="F8A29823ECD1DAE5" modified="2020-08-12" version="1">MDHHKPFSVLLVLLLHGSLQAYGTISPKVIALQQVLSSELQTLERNALLTGPLHAESSSDGSAISRLRRTFHEFERQNQESITAILRVLALIDHTLENTVQDCEKVFHLPSLYSENVLFRDVTRPLLLLVEQLCQQITLEDFGQIESSLEAVEQAATHYRSRLDETKRSMQVVLDELDHIMGTFSAPSQLNALFDQMEQVNATIRSSIARATVNLLDRQHAISDKILPTSEGNNDMFVIVESFMTFLANETGILAEDLAEQLDDWFNETTNLINIVSEEVSAIPQTLMEAPIEAFLRQESSLKCLAEYNNKKAVEKLWIGLDRILQCFHLGNDTERPFEMAKQLLSLADRNIQLTLEEILECHQSTLRVGYDAKVYDEMASCYEMVSMHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W7C9</accession>
    <name>A0A6E8W7C9_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">OAR domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8W7C9"/>
    <dbReference type="EnsemblMetazoa" id="ACON013315-RB">
        <property type="protein sequence ID" value="ACON013315-PB"/>
        <property type="gene ID" value="ACON013315"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009943"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON013315"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_036578"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="231"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="115"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="231"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="254"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON013315-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="380" mass="39431" checksum="90A17CCD23BA8854" modified="2020-08-12" version="1">MSTTLSSVSGVYPDDSEQEHGLLPDLNGVDLVMSLSPKAPNHHHHHHHHHHLTSLQHLQNLQHNSALHSHSTPFSVTDILSPIEEYRKLELTANPPSPYSGGSSVNSPSALGPNTTTGSTTTGSSSMANPYAMGPLYHSPGVQSYCAPTDNLSLAGHYTDMRNSAAAGWYGSTASDPRFAISRLMGGSAGGTMGHAMSNMGSLAACSVSDTKPMQFPLAQRRKRRVLFTQAQHPAQHHQQQQQQHTHQQQSTVGGSGAGSTGGSGGGSNGSGASGPGSNGSSSAGGGSSGTSNGSGGPASVGSIVLLNDSNAHSPDTATAALLSSYNGAAAHQHQMLQQPCNNALMSNSLAMAYRNQNNFMTNSHQQQCGGYLPLQTRAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W7E5</accession>
    <name>A0A6E8W7E5_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">mitogen-activated protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.24</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">Ngousso</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.24" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8W7E5"/>
    <dbReference type="EnsemblMetazoa" id="ACON012148-RA">
        <property type="protein sequence ID" value="ACON012148-PA"/>
        <property type="gene ID" value="ACON012148"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004243"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON012148"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032757"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006970">
        <property type="term" value="P:response to osmotic stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07851">
        <property type="entry name" value="STKc_p38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000624">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000769">
        <property type="entry name" value="Mitogen-activated protein kinase 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003527">
        <property type="entry name" value="MAP_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01351">
        <property type="entry name" value="MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="20"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON012148-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="256" mass="29424" checksum="F03DA64B3A328199" modified="2020-08-12" version="1">MPKFYRTEINKTEWEVPEKYQVLTPVGSGAYGQVCSAMDTEHNVKVAIKKLARPFQSAVHAKRTYRELRMLKHMNHENIIGLLDVFHPGANNTLESFQQVYLVTHLMGADLNNIIRTQRLSDEHVQFLVYQILRGLKYIHSAGIIHRDLKPSNIAVNEDCELKILDFGLARPTENEMTGYVATRWYRAPEIMLNWMHYNQTVDIWSVGCIMAELLTGRTLFPGTDHIHQLNLIMEILGTPNDEFMAKISSESILTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W9G4</accession>
    <name>A0A6E8W9G4_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="16">BTB domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the potassium channel family. C (Shaw) (TC 1.A.1.2) subfamily. Shaw sub-subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8W9G4"/>
    <dbReference type="EnsemblMetazoa" id="ACON029240-RA">
        <property type="protein sequence ID" value="ACON029240-PA"/>
        <property type="gene ID" value="ACON029240"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011076"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON029240"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041585"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043679">
        <property type="term" value="C:axon terminus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032590">
        <property type="term" value="C:dendrite membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032809">
        <property type="term" value="C:neuronal cell body membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042734">
        <property type="term" value="C:presynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005251">
        <property type="term" value="F:delayed rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001508">
        <property type="term" value="P:action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030431">
        <property type="term" value="P:sleep"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18416">
        <property type="entry name" value="BTB_Shaw-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000020">
        <property type="entry name" value="Potassium voltage-gated channel protein Shaw"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000002">
        <property type="entry name" value="Potassium voltage-gated channel subfamily a member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000047">
        <property type="entry name" value="Uncharacterized protein, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003968">
        <property type="entry name" value="K_chnl_volt-dep_Kv"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003974">
        <property type="entry name" value="K_chnl_volt-dep_Kv3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028325">
        <property type="entry name" value="VG_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537:SF252">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL PROTEIN SHAW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537">
        <property type="entry name" value="VOLTAGE-GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00169">
        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01491">
        <property type="entry name" value="KVCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01498">
        <property type="entry name" value="SHAWCHANNEL"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0630">Potassium</keyword>
    <keyword evidence="5" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="3" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="173"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="327"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="360"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="386"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="15">
        <location>
            <begin position="8"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="437"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="447"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00061303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON029240-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="494" mass="55931" checksum="11F39713E3A741A9" modified="2020-08-12" version="1">MNIMDSENRVVLNVGGIRHETYKATLKKIPATRLSRLTEALANYDPILNEYFFDRHPGVFAQVLNYYRTGKLHYPTDVCGPLFEEELEFWGLDSNQVEPCCWMTYTQHRDTQETLAVLERLDLDTEKPTEEELARKFGYEDDYLKGTVSWWQHMKPQMWSLFDEPYSSNAAKIIGVISVFFICVSILSFCLKTHPDMRVPVIRNITVKTANGSTAWVLDKTQTNAHVAFFYIECVCNAWFTFEILVRFISSPNKCEFVTSSVNIIDYIATLSFYVDLVLQRFASHLENADILEFFSIIRIMRLFKLTRHSSGLKILIQTFRASAKELTLLVFFLVLGIVIFASLVYYAERIQANPHNDFNSIPLGLWWALVTMTTVGYGDMAPKTYIGMFVGALCALAGVLTIALPVPVIVSNFAMYYSHTQARAKLPKKRRRVLPVEQPRQPRTAGQGGGIGMPGSGPMARRMNQVKTKDLGPKIDAASAYIGKAGGKMAQLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W9I8</accession>
    <name>A0A6E8W9I8_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">Peptide-methionine (R)-S-oxide reductase</fullName>
            <ecNumber evidence="2 7">1.8.4.12</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Methionine-sulfoxide reductase that specifically reduces methionine (R)-sulfoxide back to methionine. While in many cases methionine oxidation is the result of random oxidation following oxidative stress, methionine oxidation is also a post-translational modification that takes place on specific residues.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>L-methionyl-[protein] + [thioredoxin]-disulfide + H2O = L-methionyl-(R)-S-oxide-[protein] + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:24164"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="Rhea" id="RHEA-COMP:12313"/>
            <dbReference type="Rhea" id="RHEA-COMP:12314"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16044"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:45764"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.8.4.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the MsrB Met sulfoxide reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.4.12" evidence="2 7"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8W9I8"/>
    <dbReference type="EnsemblMetazoa" id="ACON013286-RB">
        <property type="protein sequence ID" value="ACON013286-PB"/>
        <property type="gene ID" value="ACON013286"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON013286-RC">
        <property type="protein sequence ID" value="ACON013286-PC"/>
        <property type="gene ID" value="ACON013286"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON013286-RD">
        <property type="protein sequence ID" value="ACON013286-PD"/>
        <property type="gene ID" value="ACON013286"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002452"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON013286"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030800"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033743">
        <property type="term" value="F:peptide-methionine (R)-S-oxide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030091">
        <property type="term" value="P:protein repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.150.20:FF:000004">
        <property type="entry name" value="Peptide-methionine (R)-S-oxide reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.20">
        <property type="entry name" value="Peptide methionine sulfoxide reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028427">
        <property type="entry name" value="Met_Sox_Rdtase_MsrB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002579">
        <property type="entry name" value="Met_Sox_Rdtase_MsrB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011057">
        <property type="entry name" value="Mss4-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00357">
        <property type="entry name" value="peptide-methionine (R)-S-oxide reductase MsrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10173">
        <property type="entry name" value="METHIONINE SULFOXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10173:SF52">
        <property type="entry name" value="METHIONINE-R-SULFOXIDE REDUCTASE B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01641">
        <property type="entry name" value="SelR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51316">
        <property type="entry name" value="Mss4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51790">
        <property type="entry name" value="MSRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MsrB" evidence="8">
        <location>
            <begin position="4"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON013286-PD"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="136" mass="15258" checksum="2BE3A1B772551780" modified="2020-08-12" version="1">MEKKEELVAKLTPLQYHVTQEAGTERPFTGKYNKFYEKGTYICVVCSQELFSSETKYDSGCGWPAFNDVLDQGKVTLHKDPSIPGRVRTEVRCSKCAAHMGHVFEDGPPPTRKRYCINSASIEFMPAGSERKDGTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W9J0</accession>
    <name>A0A6E8W9J0_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
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                <person name="Lambert C.C."/>
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                <person name="Galvin B.D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
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    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="2">Ngousso</strain>
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    <dbReference type="AlphaFoldDB" id="A0A6E8W9J0"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
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            <begin position="1"/>
            <end position="21"/>
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    <feature type="chain" id="PRO_5044631396" description="Secreted protein" evidence="1">
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            <end position="117"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
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</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
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    <name>A0A6E8W9J6_ANOCL</name>
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        <name type="scientific">Anopheles coluzzii</name>
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        <lineage>
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            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
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            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
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                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
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        <scope>IDENTIFICATION</scope>
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        </source>
    </evidence>
    <sequence length="2017" mass="219692" checksum="915D4BA31699D1EB" modified="2020-08-12" version="1">MALEETQNNGSAVAVAPKETVPPSLKQQQQPPQGQAQIQPSTSNEKLNNLVNGGGATTEKSRSNNKQNDTNTAFLRAARAGDLQKLIEYLETGQVTDINTCNTNGLNALHLAAKDGHYDIVNELLKRGALVDNATKKGNTALHIASLAGQKEIIQLLLQYNASVNVQSQNGFTPLYMAAQENHDECVNYLLAKGANPALATEDGFTPLAVAMQQGHDKVVAVLLESDTRGKVRLPALHIAAKKDDVKAAKLLLENEHNPDVSSKSGFTPLHIAAHYGNVNVAQLLIEKGADVNFTAKHNITPLHVACKWGKLNMVKLLIANHGRIDSITRDGLTPLHCAARSGHDQVIEVLLEHGAEIISKTKNGLAPLHMAAQGEHVSAARILLMNKSPVDDITIDYLTALHVAAHCGHVKVAKLLLDRNADPNARALNGFTPLHIACKKNRIKVVELLLNHGATIGATTESGLTPLHVASFMGCMNIVIYLLQHDASPDIPTVRGETPLHLAARAKQTDIIRILLRNGAYVNAQAREDQTPLHVASRIGNMEIVMLLLQHGAKIDAVTKDNYTPLHIAAKEGQDEVAALLLDSEANVEAVTKKGFTPLHLAAKYGNLKCAELLLERGAQVDVQGKNGVTPLHVASHYDHQKVALLLLEKGASPYSPAKNGHTPLHIASKKNQLNIATTLLDYKADANAESKTGFTPLHLSAQEGHGDMARVLLDNGADPNHAAKNGLTPLHLCAQEDHVGIAETLLEHKARIDPVTKTGFTPLHVAAHFGQAGMVKYLIENDANIEMKTNIGHTPLHQAAQQGHTLIINILLKNKANPEAVTNGGQTALSIADKLGYITVVETLKVVTETSVTQTVDEKFKIVGPETIHETFLSDSEDEGEDPIMSDQQQYNYMTSDENKQFDDTNLTNMSIDPLKDDKYLEKIISRAENYTVSAMDRSHTPNPLDITVTDNVNITRKPIHVGFMVSFLVDARGGAMRGCRHSGVRVIVPPRSAAQPTRITCRYVKPQRITNGPPLMEGEALVSRILELAPVGAKFLGPVILEVPHFASLRDKEREIIILRSDNGETWREHTLYDSEEAIHEVLNETFKGDTLNLLEDLHTNRITRIVTNDFPHYFAIVSRIRQEVHAIGPEGGTVSATAVPQVQAIFPQNALTKKIRVGLQAQPIDMNTTANLLGRSVAVSPVVTVEPRRRKFHKAITLSMPAPKAYNSGMINQYSGNAPTLRLLCSITGGQNKAVWEDVTGSTPLTFVNDCVSFTTTVSARFWLMDCRNIGEATKMATELYSQMAHVPFMVKFVVFAKRVDQSEAKLSVFCMTDDKEDKTLEHQEHFTEIAKSRDIEVCEGRTVYLEFAGNIVPVMKSGEQLSLQFNAFKENRLTFTVKIKNNLDDLLGRISFMNEPKVAKGEPIQTPLCTLNFTLPSEKFGLGGDDLETTSEFDQSSTEVLNSEQQAVVAAANRGKTLNFTFQNGDGGSEIHKADIKITDICNLLGSDWPLLADELAITPSDVELIRAEYPNDEPQQAIVMLRLWLRQAGRDATGNVLEQALIKINRPDIVNKSITNLEPVTDEYERRVAQRQIGSMNGLDEIDPARVNGMPVASSSMHESEHEPTEEKQEHEAVEKDKQLPTDNNSPTGSVSPDTTEAFQQIRRESEILGIAAIKKEDLSTPPPSPADFSTQVSQNSSKASADEGRNDGKVSSNAAVANSGQTGTVEQKAETCLAEESHPSFSVTTSGVAVIVTQVSNDLIEDEEPPQVAEEDNDDVLIDFGGGDKPNDSGVDNTDDNRATGTGGGSVLVQAGSDLAEDSTEIKNYLVQNNDQMTGMEPTEPPMESATPLNDGSTRTETNTIVDESDDGTVRTTIITTTTTLSTTGDDIPEDLEEQLLKQLRDQQLLQETQQLQQNQPQSNAQEGNQVVTITTTTAEEDPDTGVETTSTSTTRTTTTTKTITLTPDGDFPEDELLQKITTTTTHTSQQNIPEMVKETTVTVTEMVDGRTLDGAAKALNNIVDDFMNHERKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WAV0</accession>
    <name>A0A6E8WAV0_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">HP domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8WAV0"/>
    <dbReference type="EnsemblMetazoa" id="ACON029504-RA">
        <property type="protein sequence ID" value="ACON029504-PA"/>
        <property type="gene ID" value="ACON029504"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009656"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON029504"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_036288"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="215"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="277"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="446"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="517"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="122"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="217"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="227"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="281"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="326"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="456"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="517"/>
            <end position="537"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON029504-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="610" mass="65077" checksum="73E7B0DB55E285A6" modified="2020-08-12" version="1">MFGGCVGFGRSSGGGGGGTSSGGEDGTDKGLLRRSSSVRAKASMFAQLESKLKENENPLLNRPIVARPRRAQFTTQTISPTDIERSNNAINSGLSSGQYRTPPPPTTGNSTGHHHQPSAHQPIMNTIPTNNNNNNIVSDDSGAEFDPSTLQVSKKVKLFSGGCVTVKGGGGEDGVDQLTAVAALANGTANQLPAVRRRKTLLKVRTIGKLAMPKFLNDSNNNVSVRQQQQHQEQQQCNGNGSTNGTGSEGETDLLLQHIVPKVDRIRRKFMSIPSTTTTTFLNSTENGVQSPTQGGHGDDASDGSDSNVDSGKENNYDSGVENMNGPSRPSLGSGSSNVLALKRNFLNSSNRSKVSTTPQHKEKDPALDVSTELDGVVTKGKVSRLANQWNRLRMTLDVSSILKAPGTTFDTPPSPTGRIGNGLAAERECESLPIGCGEMERSYNLDSSLERSQSTHHNSTSQQQFSRSFTRKSNGSSRFALVEDRFAKYFGCKAGGQSSPAAAAAQPVSGKSMIVSRNSSVREPNTPTNNGTAGKQQSRRRSQSMPKESLLLEQRLETLISTIAANGWDGKCTVGGVQPVQTVEELNLTTEDLSMADTEFDKLFIGCCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WAW7</accession>
    <name>A0A6E8WAW7_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glyoxylate reductase/hydroxypyruvate reductase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040229688.1">
        <property type="nucleotide sequence ID" value="XM_040373754.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8WAW7"/>
    <dbReference type="EnsemblMetazoa" id="ACON013478-RB">
        <property type="protein sequence ID" value="ACON013478-PB"/>
        <property type="gene ID" value="ACON013478"/>
    </dbReference>
    <dbReference type="GeneID" id="120953628"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004050"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON013478"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030926"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030267">
        <property type="term" value="F:glyoxylate reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008465">
        <property type="term" value="F:hydroxypyruvate reductase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05301">
        <property type="entry name" value="GDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000026">
        <property type="entry name" value="Glyoxylate/hydroxypyruvate reductase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050223">
        <property type="entry name" value="D-isomer_2-hydroxyacid_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029753">
        <property type="entry name" value="D-isomer_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029752">
        <property type="entry name" value="D-isomer_DH_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996">
        <property type="entry name" value="2-HYDROXYACID DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996:SF277">
        <property type="entry name" value="GLYOXYLATE REDUCTASE_HYDROXYPYRUVATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00065">
        <property type="entry name" value="D_2_HYDROXYACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00671">
        <property type="entry name" value="D_2_HYDROXYACID_DH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase catalytic" evidence="4">
        <location>
            <begin position="14"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding" evidence="5">
        <location>
            <begin position="111"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00073306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON013478-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="321" mass="35521" checksum="5311A4963F023172" modified="2020-08-12" version="1">MKPKVYVTRNDYARIGLDLLREECDISLWDEGYPVPRDEFLKNVAGKDAIYCSLNDRIDKELLDQAGPNLKVISTISVGYDHIDVKECKQRGIRVGYTPDVLTDATAELTVALLLATARRMFEANKQVHTGGWKSWSPMWMCGTSVKNSVVGIFGFGRIGQEVAKRLAPFKPSRIQFTSRTDKFLTAEDLNVTQVPFDELIETSDFLIIACSYNVETANLFNDAVFSRMKPSAILINTSRGGVVEQHDLIHALRAGKIQAAGLDVTTPEPLPLDSPLLTLPNVVVLPHIGSADIETRIEMSRITACNILAGLKGIRMLSEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WAW8</accession>
    <name>A0A6E8WAW8_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">P-type Cu(+) transporter</fullName>
            <ecNumber evidence="3">7.2.2.8</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="17">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.8" evidence="3"/>
    <dbReference type="EnsemblMetazoa" id="ACON029519-RB">
        <property type="protein sequence ID" value="ACON029519-PB"/>
        <property type="gene ID" value="ACON029519"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000618"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON029519"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032853"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043682">
        <property type="term" value="F:P-type divalent copper transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140581">
        <property type="term" value="F:P-type monovalent copper transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055070">
        <property type="term" value="P:copper ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015677">
        <property type="term" value="P:copper ion import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="CDD" id="cd02094">
        <property type="entry name" value="P-type_ATPase_Cu-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000150">
        <property type="entry name" value="ATP7, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000001">
        <property type="entry name" value="ATPase copper transporting beta"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000002">
        <property type="entry name" value="Copper-transporting ATPase 1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000031">
        <property type="entry name" value="Probable copper-transporting ATPase HMA5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017969">
        <property type="entry name" value="Heavy-metal-associated_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006122">
        <property type="entry name" value="HMA_Cu_ion-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027256">
        <property type="entry name" value="P-typ_ATPase_IB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01525">
        <property type="entry name" value="ATPase-IB_hvy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00003">
        <property type="entry name" value="copper ion binding protein"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43520">
        <property type="entry name" value="ATP7, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43520:SF8">
        <property type="entry name" value="P-TYPE CU(+) TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00942">
        <property type="entry name" value="CUATPASEI"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01047">
        <property type="entry name" value="HMA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0186">Copper</keyword>
    <keyword evidence="9" id="KW-0187">Copper transport</keyword>
    <keyword evidence="15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="16 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="428"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="470"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="504"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="533"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="693"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="738"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1147"/>
            <end position="1169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1175"/>
            <end position="1195"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="19">
        <location>
            <begin position="52"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="19">
        <location>
            <begin position="152"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="19">
        <location>
            <begin position="261"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="19">
        <location>
            <begin position="337"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="220"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="220"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU362081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON029519-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1294" mass="139453" checksum="7032821CEB78B681" modified="2020-08-12" version="1">MNFLKNINKSSQQNGAGHGVQYALLINNGGEINGENMDDYGESTGLLEQPVASVRLPIIGMTCQSCVRNIEGTIGSKLGVIKINVVLAENAGYIDYDPSLTDPAQLAADIDDMGFECTYTEPGASSAGPKRSILVEGVESDSETTNQKSDVRTTRISIEGMTCQSCVRNIEGNMKDRPGVISIRVLLDERLGIVEYDGRQTTAEAIAEQIDDMGFEARVAGEQHQPEQQRARSSPQANGKKHATNGNLTPKKKADESALLRRCFLHVQGMTCASCVSAIEKHCRKIYGVESILIALLAAKAEVKYDERLTTPADVAKSITELGFPTEVLEEPGTGETDVEIEILGMTCGSCVAKIEQTALKIPGVLQASVALTLKRGRFKFNNERTGARTICEAIEGLGFATRVLSGKDKMAHNYLEHKEEIRKWRNAFLVSLVFGGPCMIAMVYFMVLMHERSHEEMCCVLPGLSLENLIMFTLSTPVQFVGGWHFYIQAYRAVKHGASNMDVLITMATTVSYLYSVGVLVAAMVLEQHTSPLTFFDTPPMLFIFISLGRWMEHIAKGKTSEALSKLLSLKATEATLVTLGPDYAVLSEKVISVDLVQRGDILKVVPGSKVPVDGKILCGNSTCDESLITGESMPVPKKKGAVVIGGSINQNGLLLMQATHTGEHTTLAQIVKLVEEAQTSKAPIQQLADRIAGYFVPFVVAVSVITLVGWIVSGYIDIGHIPASDRDKEGLTPSEIIVSYAFRCALSVLAIACPCALGLATPTAVMVSTGVGALHGILVKGAGPLENAHKVKTIVFDKTGTITHGMPMTSRICMLVKPAVCSLARALTIVGSAEVNSEHPIATAIVKYVKETLEIDGFGRCSNFSAVPGCGIRCVISNVGDTLKRVQQSEKMRNYQNSYRSEQQQQPLFMNGAVVEELIPQLSASQKNTIELQQLLHLEPIGEGPAGTGGDAGSGEAFADVNEYNVLIGNREWMARNAIVVPPEVNIRMSEEEQMGHTAILCAMNGQLVCMLSVSDMVKPEAHLAVYTLKRMGIEVILLTGDNKNTAASIARQVGINRVFAEVLPSHKVAKIQRIQEMGMRVAMVGDGVNDSPALAQADVGIAIASGTDVAAEAADVVLMRNDLLDVVACLDLSRKTVRKIHLNFLFASMYNLLGIPLAAGIFTPFGFTLEPWMASAAMAASSVSVVCSSLMIKLYKKPTRASLQTPEYVELMEAGAFLDPDTISVHRGLDDIPRPNLARSTSSTLAKLFGRGKDTKEDGLLATLDDDEFSVGIVKTSNASIYKDATELQKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WAX5</accession>
    <name>A0A6E8WAX5_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8WAX5"/>
    <dbReference type="EnsemblMetazoa" id="ACON013481-RD">
        <property type="protein sequence ID" value="ACON013481-PD"/>
        <property type="gene ID" value="ACON013481"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009241"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON013481"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030170"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON013481-PD"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="232" mass="27169" checksum="F0548AC83E6E2CEA" modified="2020-08-12" version="1">MVYESDFYSTRRVGSSYTRPTISSYTVTTPLRYSGVPREFEDAKSDIRNSTALLLRQLNDPVPRLMAPIAQAAPEPNPKKWVYDPFSTHNRLNSDTYVKSHITDPIRSVRNDIEAMARYHSPASRYVEYVLLDDILWRYTYYVDEEGKNHLASTRIIGSQAYPKTKPRIYNYDTAKVGKDVNVMSYYKSNRTQAKADVEEVNTKIKKYLEKYYLNHAYKVPDEFKEFLHEYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WAZ6</accession>
    <name>A0A6E8WAZ6_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
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        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
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                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
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    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
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                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.</text>
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    <dbReference type="EC" id="2.7.11.13" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8WAZ8"/>
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        <property type="protein sequence ID" value="ACON029538-PC"/>
        <property type="gene ID" value="ACON029538"/>
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    <dbReference type="VEuPathDB" id="VectorBase:ACON029538"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037802"/>
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        <property type="component" value="Unplaced"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="F:diacylglycerol-dependent serine/threonine kinase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
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    </dbReference>
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    <keyword evidence="9" id="KW-0418">Kinase</keyword>
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    <keyword evidence="7 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="33"/>
            <end position="87"/>
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            <begin position="474"/>
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        <location>
            <position position="191"/>
        </location>
        <ligand>
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    </evidence>
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        <source>
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        <source>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50011"/>
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            <dbReference type="PROSITE" id="PS50081"/>
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            <dbReference type="PROSITE" id="PS51285"/>
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        <source>
            <dbReference type="EnsemblMetazoa" id="ACON029538-PC"/>
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    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="483" mass="54744" checksum="CA80C0F61537EF0A" modified="2020-08-12" version="1">MWNLMDASLYYGSSYFVAAVTPSPVVAAGELFPNVPSAPGLSCVGEDKAFCAFCHDRIWGLGRQGFKCIQCKLLVHKKCHKLGNKPCSNEHVEPVFKERDHHDPNDESSTGDVIQAPLDYPSELHSGDGCDQVPVEVNDNVEETLEPVTQRQYSLNDFELIRVIGRGSYAKVLMVELKKTRRIYAMKVIKKALVTDDEDIDWVQTEKHVFETASNHPFLVGLHSCFQTPSRLFFVIEFVRGGDLMFHMQRQRRLPEEHARFYAAEISLALNFLHEKGIIYRDLKLDNVLLDHEGHIKLTDYGMCKEGIRPGDTTSTFCGTPNYIAPEILRGEDYGFSVDWWALGVLLYEMLAGRSPFDIAGASENPDQNTEDYLFQVILEKTIRIPRSLSVKAASVLKGFLNKNPADRLGCNRDSAFLDIMNHSFFKSIDWEMLEQKQITPPFRPRLDSDRDLANFPPEFTDEPVHLTPDDESINPSSKDSSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WB64</accession>
    <name>A0A6E8WB64_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">DEUBAD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Asx family.</text>
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        <property type="gene ID" value="ACON029590"/>
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        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
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        <property type="entry name" value="ASX-like_PHD"/>
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        <property type="entry name" value="ASX_DEUBAD_dom"/>
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        <property type="entry name" value="DEUBAD_dom"/>
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        <property type="entry name" value="ADDITIONAL SEX COMBS LIKE PROTEIN ASXL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13578:SF20">
        <property type="entry name" value="POLYCOMB PROTEIN ASX"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DEUBAD" evidence="11">
        <location>
            <begin position="389"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="23"/>
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            <end position="1003"/>
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        <location>
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        </location>
    </feature>
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            <end position="1912"/>
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        <location>
            <begin position="1941"/>
            <end position="2005"/>
        </location>
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    </feature>
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        </location>
    </feature>
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        <location>
            <begin position="2518"/>
            <end position="2561"/>
        </location>
    </feature>
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        <location>
            <begin position="2612"/>
            <end position="2674"/>
        </location>
    </feature>
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        <location>
            <begin position="2713"/>
            <end position="2762"/>
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    </feature>
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        <location>
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            <end position="3086"/>
        </location>
    </feature>
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        <location>
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            <end position="3198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="3214"/>
            <end position="3235"/>
        </location>
    </feature>
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        <location>
            <begin position="9"/>
            <end position="23"/>
        </location>
    </feature>
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        <location>
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            <end position="86"/>
        </location>
    </feature>
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        <location>
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            <end position="111"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
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            <end position="158"/>
        </location>
    </feature>
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        <location>
            <begin position="267"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="283"/>
            <end position="292"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="10">
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            <begin position="305"/>
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            <begin position="705"/>
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            <begin position="853"/>
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            <begin position="961"/>
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            <begin position="986"/>
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            <begin position="1432"/>
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            <begin position="1477"/>
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            <begin position="1554"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51916"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblMetazoa" id="ACON029590-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="3275" mass="343121" checksum="3BAC9AE69D0EB4AD" modified="2020-08-12" version="1">MECDVSPVPSVQANRTAKQQPQHIATATVLVTAGPVAAKSSATNNGSSSNSSSISSTASSATLITPSTTTTSTGTAAGSSDTVASTVDEAMAEEPSAMEPTTTMATAPATTVEDDDDATPSSSMAAGVAAPEGGDSSVKLSISSNNSNNNSSNKSLNIAGSETAGGGPLLLPPDPVAVIEISDQSMADDISYPELVSCVDTETACVVLSDEYDSSLPLREDDPLNVSVGSIDMMLPSSPQCSTPVFAGAELSSPRQGRIGTPVGNASVSTSTTGTASTTSTPKHNHHLRRNLPRLATSRQQQQQANAVKQLPQSGPGSRGGVATEKRQLRQAAAQDAGRNSGGAASTMREVLASIPGFSIKPRRRTNKKLSTAAQLEQTREGCVDLETPDSILVHTNLRALLNRDTFQLLPPLYQYKLVQLLPPVDRPPLPEATQCEQNGIRLNPSGLNNEFFARACHEWRDRLAEGEFTPEAQLKMRSEAEREKSKLDPWKLKHFEPMWGDRKYAGSFFGAAGTVANPTPPTPPPPPEPAIAVTTAMTVSVAPVVLSPALQSPPAVQVVAPSSQPIPAAAQTPLIGAGSSSSSNTLPPSVSILPVSKGVVSGHATIVNVAARQTVQPPTVAIAPQLTPRVHPVTAKIDPSARSLSKMQPITFSTAASVVRPTNAASGTCSSISSNTIITSSTGTIILPSRTTITVTSGSASKGSGSGGSIPRGGSTGSGGSAAGASGLSVVPINVLTCSSSSSSSSSSNTVNTARPALKTTIKLRPTTAIATSTTAAAAAAPTAGKESKHNHPPYSVVGSAPNASSVMVGGAGGTKRLLPTAMSVSSSSGSTTISSSTNAVNLAGASSTMKPQSNSPASSSLPMSPKRLRTVGAVTRSALAGTGTQQLPPAQHNPLPTTTTGMAAAGSTITLGSRPVVVKVVEPYRPSAMVRAVPVEPVITSGGASSNLKRTHSSSSSSNRALTPELSSSKMTKRKTDDEGGGLQQQINSSTGSFTAGSLLQHGNPRRMVAVVTASSAMADGSNISSSSSSSSNSSSNAAMYISSNNSSMNRLITATSSTTMPATSTDGVRSSSSSSSSSSSSGKRSTSSSIVGGNSNSNSSRINVSCSSTINSEGLVMINPTSIATTTTAGPGILLQAGAKARRQVECDTRTVASMATAPIDQRRMVLIEGGASEKRRRGLLTEPPVATVIGSAKRRAPRQGNRANRGRHSEGLLAARGTTAPPTTTITIVDDEEADGGSVGSPSLLVGRSEPEGEESHEVDESDENREVLRRRIVGPMVNGDFGEGARIGRAGAASSPPLVPSSDIILEEAIDCGDVGDGGGMPGSTRHIGGGRLKRSDHGGEELVETNTTSPILQETIEQANRERLMDMNHLASLNASGGGGTTQMIYDQNTGQVYSVMCLPQPTNSALGGSALNLRSLPSSLTVTALPQQQQQQQQPHHPSQQPQLSISNDSSNSSSVSTTAMMAASDNHLDGGGGAGGPGTTATSVTGISTFENVLRHHVGQGGNVDSDLLIVNPSNMMSNVELKVSEELDDVGAVMAARGDIGRTEGDDEEEDEEEEDDEGDGELVHTTTTTTNTADEEEEEDDEEEEEDVDEVEEVEAEVEEQDEEEDPDAMMLRQGLQQMHGGLEESYEDEENNAPTGCFNGELIRTSTSTATSGNKLSSYGQPNLQHLHLQQQQQQQHHPNNHLHASHHRQAAMAAGVMNMNMNLTGFHHDHELLHQQQQQQLQQLRNATAGTITSSPSASSDQGLMMMVTTSAGDEAGDGGPSSNSGDVMVMANNYCDNLSAADEADGEDEEEEEEEQYGQQRDGHDRMLLAVGDDAGLEEQAIDTIVDQYGNQLHPSGMNHLNHHDDGVMHHHLQQGSEGEEEEEDVEEASFVLDQMQQLQQQFQNQHPQQQHHHHQGLLQNHQQLVTSSDGTQVHLYPGAGGMLLDGDGLDGGASCEGVEEDQPHHYQQQQLLLKEEKMEDDQQLQQQQHLHHQQVSSDGQHHIQHQQQFTNSQHNQLMDKYIDEMDGGANDAAGSAGAYVLDPSSGEVIRTENWPQFKMKMMDSTKMMVVDHHQLVDGNNSIIISPMGTPGTTTMVSSPGAAALQQQQQQLHTTQAIHQHQHQQQQLAASQQHIISQQSPTPISMATTATIVQQLASHNALQHAQQQQQQHQQRQQQQLHSPVTSTNPTGISFLAHHAPQQPAVQTMTPVSSAAVLSSPQHPAAATTAQGLSIFQLNPQQQQQQHHSPQSVLGCGNSTIQLTSEIKDGIRNSMIKAEPGVSYTTIRQGNQLLTRIKLENDEGQLQQQQLQQQQSPQPTFQTQQQQQLPKVNVVTSSPLISLGPGGPDNLARVIESVAGNYSTTVAVGGQQQQQQLVQHQQQQPQQLIQHVQTASGHQIRYEMDPTVIQQQQATTPPQQQHLIAQQQQQQQPKFIITSRPLGTTAAGAKLPLTVQAANVIPHMQNPQFIQTQDQQLQLATSQPTVPTLQKPIQLQKIIMATPAIGQQQQAQPRPRLPLQRTQFGVQAIQPQQQQQQQQQPPSQQFQQQQQQAAPKAPQQIAPTQSQQRFQQKYVTNQLIRGQNAFLMNNVHHLQQQQQQQQVAVAAAATVGANVIVGATGNSSRSKRTTEAGSVSGAAAAGNGTATGNGSASSGSSGSSGGSSSGGSKRGGRSSSSRLPPGAVNLERSYQICQAVIQNSPNRHQLKAQLKSPQAFLAASNSNSNSSISSIGSTGSSSSSSSSSSSGVSASSIIGNNTKEDANSGSNSSGGGGNSAFGGVLGFGGNKVKVSRLVNSKRTVSAGARQPSPIVVRQAYAGGAAGAATPPSVVNASAAAGPANQSNPISIIATPQSQQQQQQLHTIGEPLQQHGQQIISVSAAPTIVHATAAASNNNGTVGGNDNVGVTAAAGATPTGAAAAAGTFGGKYVLVQRAAHIGDIVTPRAASAPPTHNQIQIHHVPVSPHTHQQQQQQQQQLHQQMSAVQQQQLSQQVVQHQLQQQQQHQFQQQQGQLQQIAAGANAVAPASLQAAITRRIPSTHVITYGQDDSGGTEAEDGTGTNNAIVYMDSPNALIGGLQHQQQQQQQQQQQQQQEQAFLDGCGNGPGGANSIVVMNGGDTMEPPAGVNEMMDASHSSASDGAAAGMLVSPSDISPHFNNQMIVAGHMELLDSGYINNDAGNHVETIEPQQQQQQQQQQEPISSTEHDIDTLNCCSSPAALVDGGGGNAADNGGGEDRDGMGERNDGAGEDCSCSLNAMVICQQCGAFCHDDCVSATKLCVSCVVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WB72</accession>
    <name>A0A6E8WB72_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Rab-GAP TBC domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_049463723.1">
        <property type="nucleotide sequence ID" value="XM_049607766.1"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON029118-RA">
        <property type="protein sequence ID" value="ACON029118-PA"/>
        <property type="gene ID" value="ACON029118"/>
    </dbReference>
    <dbReference type="GeneID" id="120951704"/>
    <dbReference type="CTD" id="40704"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009384"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON029118"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042270"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01269">
        <property type="entry name" value="PTB_TBC1D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000043">
        <property type="entry name" value="Pollux, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000001">
        <property type="entry name" value="TBC1 domain family member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2750:FF:000002">
        <property type="entry name" value="TBC1 domain family member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021785">
        <property type="entry name" value="DUF3350"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006020">
        <property type="entry name" value="PTB/PI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF16">
        <property type="entry name" value="GTPASE ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11830">
        <property type="entry name" value="DUF3350"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00640">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00462">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="5">
        <location>
            <begin position="799"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="100"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="209"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="599"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="687"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1243"/>
            <end position="1291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1323"/>
            <end position="1357"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="1073"/>
            <end position="1128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="121"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="222"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="267"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="307"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1256"/>
            <end position="1272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1333"/>
            <end position="1345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON029118-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1357" mass="152256" checksum="F529DBC71ABB9E0F" modified="2020-08-12" version="1">MFTLPLYKDNVMVTSWSDASSYRMKKNMAELMQQMRDPATVGGGSVGSIPQSIVSSSANANDLQKALRNQGIHSTPASNLKLSEQMRYAQHDSSPTLAYHLQQHQQQQHQHYQSYPQQYQNGAPNRLATSKSYSGGLNHSSGGVNGPTGSGGISCSLASLPDISANNSQFFEVLYVGKIKVSHKRVPYTFIDEALPKFKAYDAEKLRQQQEASRKQNVPINAESVSSSTAEAATTQRSNEAENKGTESTPPAAPDDPPEVFQGRRVSHFDIPTKKIENGTVGESNTSGIVKPAEEQAKIGESTTNEQHQQQQQQQQQQQPQSATTATVAPPNTERVVASNKESKSASPQDEDAPMRRDRSASIGSIPVVEQNRTMVFLIGQSDLRLISPDRKQVLLHKAFRDVASCVHGQKNADHFGIICRDVNNDGYIGYVFKCQSEQVADDIVTAISLAFTACSEQSQKHKPAAQIFSCEHCPMLWYHKLCTDTEGLSDKKTQSTIFKRIETTLTEDEQRSLNEKYHGAEEAAGSSLGEQNQFLMMLLRAHCESKQQRHVHDTVEHRTEFLNQYLGGSGIFMKAKRSLTSSFDHLLKRRTSRDDYVAGSLKDSNSAGELKEGSFEPVTRARSSTIGSSPGMGRKNSDLGNGTAPPLKSPMMDIFIKVGNSPRDQGHAGSWRQAMLHRVVTPSKNMRNGSDEYMSPLRAVGGAPAKKRTREELRELWRVGIKQTIILSRMEKENAHLQARQNEIKSNEMRRVKLEYDEIAVCDKRSAEQWDTLLDSGTAAGTATPTNQAVLYQAMRNGVPRSRRGEIWMYLAEQHGLHHPDPIDTTNFPNFNTPYHVLLKSLTEHQHAIFIDLGRTFPDHKYYKDALGVGQLSLFNLLKAYSILDPELGYCQGLGFICAVLLLHLEEADAFELLKHLMFRRQLRAKYLPDMRQFQLQLYQLERLLKDHIPELFQWFNQHDISPTLYAAPWILTVFSSHFPLGFVVRVFDLIFLDSFDVFFRCAIALLEVHKEELLQRDNFEDIMSYLKTVIPKIDADVMEKVFRNVFRSDFSRQLIVSQVEYNVMQEEITCQNHYQESYNRLKEEHQQLDTQLQYAQSNLLQLEKTRLVQQDQIQSLQTQVQTLENTVHTLVRYIEQSTDAVLPSDVCRIVQQVQDLQLQQHQQQQQKKRSPIFVDRKIGKSISFNSNLGLALNVLEEATEPDGPGTPTKKKQPYFQNSFTQIRQQRASLWLHKLDECNTSPEHGSAGSVASGMSLPSSTSLKGLGSNNDDSGIATPISPQLHAPSTTPMVAESRPLVPSFENHPLSSCGDVAVQYNGTTQLKSLKPRSQSRHGSVSLIVSETATEPRPQVATDRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WB94</accession>
    <name>A0A6E8WB94_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DUF4806 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8WB94"/>
    <dbReference type="EnsemblMetazoa" id="ACON028444-RB">
        <property type="protein sequence ID" value="ACON028444-PB"/>
        <property type="gene ID" value="ACON028444"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006409"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON028444"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031385"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="108"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="141"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="193"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON028444-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="270" mass="30653" checksum="C39460047D3E0E96" modified="2020-08-12" version="1">MNKYQTKLVAGTGTGAAVLTEQVDPLGKNTQDERKKPTAVGSITREVASNSNDNRQYTDAMKLDPSRIEIESVLANAIKIENFTEEDSVFNEESIPLDTIRLPKRKYFGDEESEDDSSITSKSQDSNDAWDEMHYQKETDLRATASTANNPVKKKKLEQTPRESVKQYLVNDSFRMKPVSEPNRNRISQNDGVQDISIQIQICKERIRKLEEKNRYLKQVIADKLDIIVKGSTRQVQQMEKLVSTRAGSPLLEPFPDPSFTAKLSTPNRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WB96</accession>
    <name>A0A6E8WB96_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Suppressor of cytokine signaling 5</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8WB96"/>
    <dbReference type="EnsemblMetazoa" id="ACON029624-RA">
        <property type="protein sequence ID" value="ACON029624-PA"/>
        <property type="gene ID" value="ACON029624"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006902"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON029624"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037038"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005942">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046935">
        <property type="term" value="F:1-phosphatidylinositol-3-kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009968">
        <property type="term" value="P:negative regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000028">
        <property type="entry name" value="Suppressor of cytokine signaling 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001496">
        <property type="entry name" value="SOCS_box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036036">
        <property type="entry name" value="SOCS_box-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10155">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3-KINASE REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10155:SF0">
        <property type="entry name" value="SUPPRESSOR OF CYTOKINE SIGNALING AT 36E, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07525">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00253">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00969">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158235">
        <property type="entry name" value="SOCS box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50225">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0341">Growth regulation</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="3" id="KW-0734">Signal transduction inhibitor</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="SH2" evidence="8">
        <location>
            <begin position="604"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="domain" description="SOCS box" evidence="9">
        <location>
            <begin position="694"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="15"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="147"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="307"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="392"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="18"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="74"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="88"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="147"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="220"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="231"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="312"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="323"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="394"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="405"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON029624-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="759" mass="79001" checksum="1B0CBD1E0D074623" modified="2020-08-12" version="1">MAHKRFLKMFWPFQDCQSTSSSPTAGSSAVASPSAATVGGSTVAATSNNNGSSAKDAEQRPGEATVDDNSSNQTAAAPTTPTSVVSAGSANTASDSSPVATVTVGSASKATANIPPCASSVSCCASNGNSRKSASSTASTLFGSLSFAGRRSRSSNARAGSCCSPTADGCNSPEQQQQQQRSRISWIPGSGQKQHRNGAKNATPSAKAKKSNWPFSLKSSGGGGGSGGSNTGTAAAAAHKQTTKAASGGSVVSTNTAMSSDATAGGSGSVLAGVAGPSLRGSTLIASAKLLKGESDKDDTCVCTAYRKVSESPPPPSSPVPPSAAAAAAGPSGVAAPRPNESVEWLEEAIQGSGLVPLNLHMLGSLRAAGSAGTSAGQPLISLAGPESIPMLPNNGSSSSSSNNNHHHHHHHHAMAAALVADHHPTFFFQPAHHAPAVAAGAPGAAAAAAAGPAALFGTLPLGAGHPPANDVILLADSPVLFDLTSDDFSVEDCDERARIQRELEIREGVDAPPNFHPRRLMSGGTIASGPAANHPIAFIYSRDLVQRGLLMANKLIQPLDAAAAAAAGVCVARSGFGRLRIHSQVDFIHCLVPDLQKITSCSFYWGKMDRYEAERLLEGKPEGTFLLRDSAQEEFLFSVSFRKYNRSLHARIEQFNHKFSFDSRDPGVFTASTVTGLLEHYKDPSCVMFFEPMLTFPLNRNFSFSLQQLCRAVIVSNTTYDGINELTLPKSLKSYLKEYHYRQRVRFRPMDEHLYSNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WCZ3</accession>
    <name>A0A6E8WCZ3_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="EnsemblMetazoa" id="ACON029489-RB">
        <property type="protein sequence ID" value="ACON029489-PB"/>
        <property type="gene ID" value="ACON029489"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007660"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON029489"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038149"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
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</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABM2AHQ5</accession>
    <name>A0ABM2AHQ5_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">Histone-lysine N-methyltransferase, H3 lysine-79 specific</fullName>
            <ecNumber evidence="2 11">2.1.1.360</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9 11">Histone H3-K79 methyltransferase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Histone methyltransferase that specifically trimethylates histone H3 to form H3K79me3. This methylation is required for telomere silencing and for the pachytene checkpoint during the meiotic cell cycle by allowing the recruitment of RAD9 to double strand breaks. Nucleosomes are preferred as substrate compared to free histone.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>L-lysyl(79)-[histone H3] + 3 S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl(79)-[histone H3] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:60328"/>
            <dbReference type="Rhea" id="RHEA-COMP:15549"/>
            <dbReference type="Rhea" id="RHEA-COMP:15552"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="EC" id="2.1.1.360"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="11">In contrast to other lysine histone methyltransferases, it does not contain a SET domain, suggesting the existence of another mechanism for methylation of lysine residues of histones.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the class I-like SAM-binding methyltransferase superfamily. DOT1 family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_040373799.2"/>
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        <property type="protein sequence ID" value="ACON003282.P215"/>
        <property type="gene ID" value="ACON003282"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20902">
        <property type="entry name" value="CC_DOT1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.60">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025789">
        <property type="entry name" value="DOT1_dom"/>
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        <property type="entry name" value="H3-K79_meTrfase"/>
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    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
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    <dbReference type="PANTHER" id="PTHR21451">
        <property type="entry name" value="HISTONE H3 METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE, H3 LYSINE-79 SPECIFIC"/>
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        <property type="entry name" value="DOT1"/>
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    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
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        <property type="entry name" value="DOT1"/>
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    <keyword evidence="7 11" id="KW-0156">Chromatin regulator</keyword>
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    <sequence length="2547" mass="269165" checksum="FD84B5EA57B802E0" modified="2025-10-08" version="1">MATPNYKELKLQSPAGAEPFLYNFPFSTVMGTGHDSGAELIENVRWVCEDMPEIKSAIEEIDLNNLDTNNYDAMKNLCDRFNRAIDSVAALEKGTSLSNQRFTYPSRGLLKHILQQVYNQAVVEPEKLNQYEPFSPEVYGETSFDLICQMIDQVKITADDVFVDLGSGVGQVVLQMAASTPVKVCFGIEKADVPSKYAEGMNTTFKLWMRWFGKKYGDYELIKGDFLADEYREKITSATIVFVNNFAFGPNVDHQLKERFADLRDGARIVSSKSFCPLNFRITDRNLSDIGTIMHVSEMSPLRGSVSWTGKPVSYYLHIIDRTKLERYFQRLKTKGTENHTDGTSGGGSGSHSTRSSRSRKDNNTNHHHHHPKVITNDDSTSESDTDVVGPTTRKAWSDWNSGKEGKTSPSEEENNNSPVLRNGRIPVATKKRRKITRTKAAGKKAELAAASAAAAAAAAAAAAAANRDMGVGTSAASAAAAAAAAMAGGKKRGRVKGKGRQRRPLNIAGLDLLHNETLLSTSEQMIGKRLPPAPGCVDQQLTSLAGDMQHNELDIPEAPSETPYALQILLDLYKTQFMKTIEAMRKPSYKDNVQQQFDREKERNQRLMNRAGQLEKQIKVLIDDSVALLKARMNELGISTTSQNDLLCKAKEIVGRHKELQVMAAKLQNQVNVIEQEQKRLVMQHLTKLTVEQQQQHQQQQQHQQLHPHQPYIKQEDAELTSSSSNELVLKAIASTLSQRKKLYAQVSNLETELNLIEKLTEERKSMVVGLSAAAPNSNHNSASSTAAAAAAAAATSTIISVARATEVRDRELYGHAGQLHPATIGGGREREHIHPDGTTTSKHTSDPVRTLPAGAAAGPVGVAGSAAPLPPPPMAGVGSASVTVPSTPTKQGSGGGSSRSAQRKSRENRTRSQEWPEIPDIGKIEENNPEILAQKILETGRQIEAGKLLAAGKHASKERGSEGKLAPVVTAGHGSSAAAGTAAPQHIAHPPTVGAPAPQQQPYPHHHQQPHSQPSQPPVHPHLHHPDTALMPAPASTINKAHHHHRSNSGGPSGAVPPSVPTGGGSLPKCYVPGTASNEHHSGGGRNAAEGASGTGRGGSSGGGGGSGKLQDSHKVVNFEDRLKSIITSVLQGSPKTGNTASSAPAPVSLTVTGPPPAGPSPGAGHRDAHHRSGSGGHQPLTMEPAGSPLKATSASGAGYGSAAGPGKTTVYLQSSPGAGSHHPHQMVVAQDMSARSSSGGRGPSPSAHNPAHQQQIHPHQVSQSQYQQQQQLLHHPHHPHAQQSLHHQQQQHQHYQMQQQQQHQHQQQQQQGMLNVITSGAHHLNASTSISTSPVPTSPYKMHPTGPQASSIAGSASTKISPSSKYPYQKGGPVAGMSHHSPGSSALSTHQQIIQQQERERAMLYAAAAHGGGLPIDHPHHPLHQHHHRGMPPSLVDGKMLEFKAPENFRYDPRASGPSGAAGNGPPMLDTSAVSLQSHSRSSSTNSLDSIPAADYGPASAGASAVGSGGARYGGQQQQPIPLVTHSPGVGSQGSGPQHGGNNSRPGSTSSQPDYTQVSPAKMALRRHLSQEKLTHPSAGGPAAGGPVSGGPGAGGSGGGLGTVKTIGDLVNGEIERTLEISNQSIINAAINMSSHQQQQQQSSAASGNSSAPPASGNNTVINTHVQRPERVSIRLLEEAGHAAAGGPPPPGSSGGTYSPISRPGSVGDSGSKSPVHHLHGQSNLASLVQVSAYNSKNQKGAGTTAVPSTIVSPRGGGSSQQQQHHQQYSTAPGSGAAGGPGAVYQQSTSRGHDRHHSGEPMPYMALPRADMKPYLESYFTDEHNKRQQQLHQQQQQHQQHPQHQQQHLQHPVQSPSPSAMSASAMGLHHHQQQQMHHQHAPLAMHRASHPVDLHRGSVVMNEPGMLSRSRGEMIPMDDNRMDRLNGGPPLEGLAASLQARVIATLKIKEEDEERHRRDLSIHHSTSGTINSSSNSINSTNSNIHGGSLQIVQTAHIKSEKYTSSSSSSSTSSSSSTSSHHHHALKRTSPIVEHPAGTRPPKMLYTTSASAGGMDCGPDADMLHVPRGAVPGSSVVGHSAVRGGLLVAPPLVMSPEINSLSSVVDDGRHHHQLHVRHNHHSRNDVDDDVVIGDDESSWHDRVSSGFDRLVAFASTELDKTRRSNEDAPASSASCTTSPDSGINQSDHSRTFLSSSSSSSQLDVPPGSASVGSSSSSSISSSSSSNSSTSSIGSVTGGHGGGSGGSHGVGGHAGVPGGLMMKHMVPIIKSSPAEPVDSPPLSDVGLPRTPSPTSSPPLLFGHPTASSTAIAGGATTSAVSTVMQPSLLHPPVVGGHAPVAGSGNNNGVVTPAAGSAAPVNNSNSGNSSSSSSSLGIPLKYQRQSKSSSSSSEKHYKKKFRERNWEEYEESLSGGRNSAISGSGDVPMDQQDYHHTVASLNAPHSGSMDVGNASAPSSTSMSAEPIGPAVGDKRNDDHAAQHHHQQQQQQQQQQQQQQQHHHKHKSAKFRPKGKDWNWDDEHLNASSGSATSTRGAGRSGTNTATT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABM2AHR3</accession>
    <name>A0ABM2AHR3_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">Histone-lysine N-methyltransferase, H3 lysine-79 specific</fullName>
            <ecNumber evidence="2 11">2.1.1.360</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9 11">Histone H3-K79 methyltransferase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Histone methyltransferase that specifically trimethylates histone H3 to form H3K79me3. This methylation is required for telomere silencing and for the pachytene checkpoint during the meiotic cell cycle by allowing the recruitment of RAD9 to double strand breaks. Nucleosomes are preferred as substrate compared to free histone.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>L-lysyl(79)-[histone H3] + 3 S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl(79)-[histone H3] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:60328"/>
            <dbReference type="Rhea" id="RHEA-COMP:15549"/>
            <dbReference type="Rhea" id="RHEA-COMP:15552"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="EC" id="2.1.1.360"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="11">In contrast to other lysine histone methyltransferases, it does not contain a SET domain, suggesting the existence of another mechanism for methylation of lysine residues of histones.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the class I-like SAM-binding methyltransferase superfamily. DOT1 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.360" evidence="2 11"/>
    <dbReference type="EnsemblMetazoa" id="ACON003282.R214">
        <property type="protein sequence ID" value="ACON003282.P214"/>
        <property type="gene ID" value="ACON003282"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20902">
        <property type="entry name" value="CC_DOT1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025789">
        <property type="entry name" value="DOT1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030445">
        <property type="entry name" value="H3-K79_meTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21451">
        <property type="entry name" value="HISTONE H3 METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21451:SF0">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE, H3 LYSINE-79 SPECIFIC"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DOT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DOT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 11" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
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            <begin position="18"/>
            <end position="333"/>
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        <location>
            <begin position="1384"/>
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        <location>
            <begin position="1424"/>
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        </location>
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            <begin position="1457"/>
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            <dbReference type="RuleBase" id="RU271113"/>
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            <dbReference type="SAM" id="Coils"/>
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        <source>
            <dbReference type="PROSITE" id="PS51569"/>
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            <dbReference type="EnsemblMetazoa" id="ACON003282.P214"/>
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        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
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    <sequence length="2138" mass="227407" checksum="50810BB6BB1C60FC" modified="2025-10-08" version="1">MATPNYKELKLQSPAGAEPFLYNFPFSTVMGTGHDSGAELIENVRWVCEDMPEIKSAIEEIDLNNLDTNNYDAMKNLCDRFNRAIDSVAALEKGTSLSNQRFTYPSRGLLKHILQQVYNQAVVEPEKLNQYEPFSPEVYGETSFDLICQMIDQVKITADDVFVDLGSGVGQVVLQMAASTPVKVCFGIEKADVPSKYAEGMNTTFKLWMRWFGKKYGDYELIKGDFLADEYREKITSATIVFVNNFAFGPNVDHQLKERFADLRDGARIVSSKSFCPLNFRITDRNLSDIGTIMHVSEMSPLRGSVSWTGKPVSYYLHIIDRTKLERYFQRLKTKGTENHTDGTSGGGSGSHSTRSSRSRKDNNTNHHHHHPKVITNDDSTSESDTDVVGPTTRKAWSDWNSGKEGKTSPSEEENNNSPVLRNGRIPVATKKRRKITRTKAAGKKAELAAASAAAAAAAAAAAAAANRDMGVGTSAASAAAAAAAAMAGGKKRGRVKGKGRQRRPLNIAGLDLLHNETLLSTSEQMIGKRLPPAPGCVDQQLTSLAGDMQHNELDIPEAPSETPYALQILLDLYKTQFMKTIEAMRKPSYKDNVQQQFDREKERNQRLMNRAGQLEKQIKVLIDDSVALLKARMNELGISTTSQNDLLCKAKEIVGRHKELQVMAAKLQNQVNVIEQEQKRLVMQHLTKLTVEQQQQHQQQQQHQQLHPHQPYIKQEDAELTSSSSNELVLKAIASTLSQRKKLYAQVSNLETELNLIEKLTEERKSMVVGLSAAAPNSNHNSASSTAAAAAAAAATSTIISVARATEVRDRELYGHAGQLHPATIGGGREREHIHPDGTTTSKHTSDPVRTLPAGAAAGPVGVAGSAAPLPPPPMAGVGSASVTVPSTPTKQGSGGGSSRSAQRKSRENRTRSQEWPEIPDIGKIEENNPEILAQKILETGRQIEAGKLLAAGKHASKERGSEGKLAPVVTAGHGSSAAAGTAAPQHIAHPPTVGAPAPQQQPYPHHHQQPHSQPSQPPVHPHLHHPDTALMPAPASTINKAHHHHRSNSGGPSGAVPPSVPTGGGSLPKCYVPGTASNEHHSGGGRNAAEGASGTGRGGSSGGGGGSGKLQDSHKVVNFEDRLKSIITSVLQGSPKTGNTASSAPAPVSLTVTGPPPAGPSPGAGHRDAHHRSGSGGHQPLTMEPAGSPLKATSASGAGYGSAAGPGKTTVYLQSSPGAGSHHPHQMVVAQDMSARSSSGGRGPSPSAHNPAHQQQIHPHQVSQSQYQQQQQLLHHPHHPHAQQSLHHQQQQHQHYQMQQQQQHQHQQQQQQGMLNVITSGAHHLNASTSISTSPVPTSPYKMHPTGPQASSIAGSASTKISPSSKYPYQKGGPVAGMSHHSPGSSALSTHQQIIQQQERERAMLYAAAAHGGGLPIDHPHHPLHQHHHRGMPPSLVDGKMLEFKAPENFRYDPRASGPSGAAGNGPPMLDTSAVSLQSHSRSSSTNSLDSIPAADYGPASAGASAVGSGGARYGGQQQQPIPLVTHSPGVGSQGSGPQHGGNNSRPGSTSSQPDYTQVSPAKMALRRHLSQEKLTHPSAGGPAAGGPVSGGPGAGGSGGGLGTVKTIGDLVNGEIERTLEISNQSIINAAINMSSHQQQQQQSSAASGNSSAPPASGNNTVINTHVQRPERVSIRLLEEAGHAAAGGPPPPGSSGGTYSPISRPGSVGDSGSKSPVHHLHGQSNLASLVQVSAYNSKNQKGAGTTAVPSTIVSPRGGGSSQQQQHHQQYSTAPGSGAAGGPGAVYQQSTSRGHDRHHSGEPMPYMALPRADMKPYLESYFTDEHNKRQQQLHQQQQQHQQHPQHQQQHLQHPVQSPSPSAMSASAMGLHHHQQQQMHHQHAPLAMHRASHPVDLHRGSVVMNEPGMLSRSRGEMIPMDDNRMDRLNGGPPLEGLAASLQARVIATLKIKEEDEERHRRDLSIHHSTSGTINSSSNSINSTNSNIHGGSLQIVQTAHIKSEKYTSSSSSSSTSSSSSTSSHHHHALKRTSPIVEHPAGTRPPKMLYTTSASAGGMDCGPDADMLHVPRGAVPGSSVVGHSAVRGGLLVAPPLVMSPEINSLSSVVDDGRHHHQLHVRHNHHSRNDVDEGTTATYYH</sequence>
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<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Anopheles coluzzii</name>
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    </organism>
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        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
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    </reference>
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</entry>
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    <accession>A0ABM2BCC6</accession>
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        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
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            <strain evidence="2">Ngousso</strain>
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            <begin position="181"/>
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        <source>
            <dbReference type="EnsemblMetazoa" id="ACON029109.P223"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="228" mass="23506" checksum="BE9E71C3E22C64A7" modified="2025-10-08" version="1">MVNSTATTPSQSHGGALYYVVEVPREEYLKECAAQATAMASAGGYPNPTYQAQYAAHHQQFQNVPNTTILARLNSTESGWDNPFRPGGDLSREADEIVNLIKGGKPITPTGDQSLVNGSTPKDSHSTDDPDTTVVDGAVAKHEASQLQAAAAQQNGTKSPHKSPGTAAGGAAGSPALGAKNGTTGADGSTVHSSTTPISNQVIPGPQSASHVVIDEKKKKKCTCCVIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-11-02" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1C7CZB5</accession>
    <name>A0A1C7CZB5_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Maleylacetoacetate isomerase</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>4-maleylacetoacetate = 4-fumarylacetoacetate</text>
            <dbReference type="Rhea" id="RHEA:14817"/>
            <dbReference type="ChEBI" id="CHEBI:17105"/>
            <dbReference type="ChEBI" id="CHEBI:18034"/>
            <dbReference type="EC" id="5.2.1.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>glutathione</name>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 5/6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the GST superfamily. Zeta family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A1C7CZB5"/>
    <dbReference type="STRING" id="1518534.A0A1C7CZB5"/>
    <dbReference type="EnsemblMetazoa" id="ACOM042483-RC">
        <property type="protein sequence ID" value="ACOM042483-PC.1"/>
        <property type="gene ID" value="ACOM042483"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACOM042483-RD">
        <property type="protein sequence ID" value="ACOM042483-PD.1"/>
        <property type="gene ID" value="ACOM042483"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON002898-RB">
        <property type="protein sequence ID" value="ACON002898-PB"/>
        <property type="gene ID" value="ACON002898"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON002898-RC">
        <property type="protein sequence ID" value="ACON002898-PC"/>
        <property type="gene ID" value="ACON002898"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_013990"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACOM042483"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002898"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037102"/>
    <dbReference type="UniPathway" id="UPA00139">
        <property type="reaction ID" value="UER00340"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000075882">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
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    <dbReference type="GO" id="GO:0016034">
        <property type="term" value="F:maleylacetoacetate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006559">
        <property type="term" value="P:L-phenylalanine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006572">
        <property type="term" value="P:L-tyrosine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03191">
        <property type="entry name" value="GST_C_Zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03042">
        <property type="entry name" value="GST_N_Zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000010">
        <property type="entry name" value="Maleylacetoacetate isomerase isoform 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Maleylacetoacetate isomerase isoform 1"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005955">
        <property type="entry name" value="GST_Zeta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034330">
        <property type="entry name" value="GST_Zeta_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034333">
        <property type="entry name" value="GST_Zeta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01262">
        <property type="entry name" value="maiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42673">
        <property type="entry name" value="MALEYLACETOACETATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42673:SF4">
        <property type="entry name" value="MALEYLACETOACETATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13410">
        <property type="entry name" value="GST_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13409">
        <property type="entry name" value="GST_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-0585">Phenylalanine catabolism</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0828">Tyrosine catabolism</keyword>
    <feature type="domain" description="GST N-terminal" evidence="12">
        <location>
            <begin position="7"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="13">
        <location>
            <begin position="95"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002898-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="219" mass="25070" checksum="D04954A999A8F62D" modified="2016-11-02" version="1">MSLSAMSKPILYSYWRSSCSWRVRIALNLKEIPYDIKPISLIKSGGEQHCNEYREVNPMEQVPALQIDGHTLIESVSIMYYLEETRPQRPLMPQDVLKRAKVREICEVIASGVQPLQNLIVLIHVGEEKKKEWAQHWITRGFRAIEKLLSTSAGKFCVGDEITLADCCLVPQVFNARRFHVDLRPYPIILRIDRELEGHPAFRAAHPSNQPDCPPEAAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V1Q7</accession>
    <name>A0A6E8V1Q7_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Exosome complex component 10 homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the exosome component 10/RRP6 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8V1Q7"/>
    <dbReference type="EnsemblMetazoa" id="ACON002300-RA">
        <property type="protein sequence ID" value="ACON002300-PA"/>
        <property type="gene ID" value="ACON002300"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_008147"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002300"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032943"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003727">
        <property type="term" value="F:single-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000467">
        <property type="term" value="P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071044">
        <property type="term" value="P:histone mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071040">
        <property type="term" value="P:nuclear polyadenylation-dependent antisense transcript catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071039">
        <property type="term" value="P:nuclear polyadenylation-dependent CUT catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071035">
        <property type="term" value="P:nuclear polyadenylation-dependent rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071036">
        <property type="term" value="P:nuclear polyadenylation-dependent snoRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071037">
        <property type="term" value="P:nuclear polyadenylation-dependent snRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071051">
        <property type="term" value="P:poly(A)-dependent snoRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06147">
        <property type="entry name" value="Rrp6p_like_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000059">
        <property type="entry name" value="Exosome complex exonuclease Rrp6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.80:FF:000001">
        <property type="entry name" value="Putative exosome component 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.80">
        <property type="entry name" value="HRDC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002562">
        <property type="entry name" value="3'-5'_exonuclease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012588">
        <property type="entry name" value="Exosome-assoc_fac_Rrp6_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010997">
        <property type="entry name" value="HRDC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002121">
        <property type="entry name" value="HRDC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044876">
        <property type="entry name" value="HRDC_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045092">
        <property type="entry name" value="Rrp6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049559">
        <property type="entry name" value="Rrp6p-like_exo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12124:SF47">
        <property type="entry name" value="EXOSOME COMPONENT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12124">
        <property type="entry name" value="POLYMYOSITIS/SCLERODERMA AUTOANTIGEN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01612">
        <property type="entry name" value="DNA_pol_A_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00570">
        <property type="entry name" value="HRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08066">
        <property type="entry name" value="PMC2NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00474">
        <property type="entry name" value="35EXOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00341">
        <property type="entry name" value="HRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47819">
        <property type="entry name" value="HRDC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50967">
        <property type="entry name" value="HRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="5" id="KW-0271">Exosome</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="HRDC" evidence="12">
        <location>
            <begin position="478"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="674"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="720"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="829"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="268"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="723"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="843"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="875"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="954"/>
            <end position="970"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="979"/>
            <end position="988"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00043957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00070365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002300-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="988" mass="111326" checksum="CA997F43AB5FEA47" modified="2020-08-12" version="1">MTDNKKQKKAGKSNAQAAESNGIEMGSPSIKNDQASDNEGIREMCEQGQNAIIAGMKAANAMPSGRSRDLYAAHPEFIKIMDTRANQVLHIIANILQLQGVQGNILHRDPDERLEMIHDFNDNILERIHSNLDEMAGIRKVVPTVLVQSEVQVPVTPRYRLSGAWNERQKGEPVKATLITGTNIARPQVKFKVPVDNSRLNPFVPKIRDKPHSLKPLAVLPEYDEAGNIVSYLHPYEFELDRFQPAKHVFERTTPQEPVPLERTPLMYVDQESQLAELVRELQAAKEIAIDLEHHSYRSYQGFTCLMQLSTRTKDYIVDALALRDELHVLNEVFTDPKKLKVLHGSVSDIEWLQRDLGLYLVNMFDTGEAARVLQFSRIGLQFLLKHYCNIDTDKAFQLADWRIRPIPENFIEYARKDTHYLLYIYDRMRNELLEKGESLLQTVYDKSTFMCKQRYQKPTMNEDTVMNIYRRSRYVFDHRQMYAFREVLYWRDQIARLEDESPGYVLPQHMALDIASKLPREMQGIIACCTPVPSLVRQHLHALHKIVLKARELPAVAGGNQAQTKEQHADTRYKMHSAFDFDNPLVCPHDDAGHGTDDAGATNRPTLLGDLTNRMEATKPVPAINANLLKDVPDMGVLAVPKIRTIDTRGHVGLSESDVSEAKLTKLLALHTNHPQQDQSEDAGSENKASKYEQNKFNTPFERYLETCRLRQLKERNDEALQKQQNTGATSKQSTETPSADVLTLQQPNPPAPATVVVVKEEPSQKAPPQLLTQNQLKRLEEAKERKSIAADEKLCFNGTVIMEKPANQTPGKRKSFPMEINVSSSSDAIEQDLQPVAGEQKPADKLHMEPFKDQSKPAGPHNDSANWSEKPAKSKKDKQRQRNKAKSSVGQGGGRQSSNKPVVPFDYNNVDFSRFQGGSKPLAGPRKGKRPGFAFTLADDPNEGSSGNNTNKKLHPNSRIAKNIKKTQKMFNLSTSGGHGGGGKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V1R7</accession>
    <name>A0A6E8V1R7_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Autophagy-related protein 16 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat ATG16 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8V1R7"/>
    <dbReference type="EnsemblMetazoa" id="ACON002315-RC">
        <property type="protein sequence ID" value="ACON002315-PC"/>
        <property type="gene ID" value="ACON002315"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001863"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002315"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040904"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034274">
        <property type="term" value="C:Atg12-Atg5-Atg16 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000421">
        <property type="term" value="C:autophagosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043495">
        <property type="term" value="F:protein-membrane adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22887">
        <property type="entry name" value="Atg16_CCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045160">
        <property type="entry name" value="ATG16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013923">
        <property type="entry name" value="Autophagy-rel_prot_16_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19878">
        <property type="entry name" value="AUTOPHAGY PROTEIN 16-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19878:SF8">
        <property type="entry name" value="AUTOPHAGY-RELATED 16, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08614">
        <property type="entry name" value="ATG16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Autophagy-related protein 16" evidence="6">
        <location>
            <begin position="12"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="254"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="298"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="79"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002315-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="341" mass="38170" checksum="89B213502ED1A745" modified="2020-08-12" version="1">MAKMQENWRDIIADRLRERNKNESARFADIIHYNNRLFDTAAKLRQENLDLKIDRKIRDSVNVSGAGGGGGTTFGTDVATITNAQIQSLEKKVLAQQEELTDLHKRKGEHSQMVITLNQKLTELQRLLAEKERALADQTALCASLKEELDEMKASHVTIKDESLASQLRANTAEDKLRAVQDENTKLLNLLMEYKSRDAEIMNKENDKFVKEKKARIESELERAIRDIPGPSSIGADLDRFDVDGLPEGVEFKFDAHDGEVNAVRWSPIERIVATGGADRKVKLWDVGKGVCEQRGVLVGSNQGINSVEFDTTGSMILAASNDSASRMWSVVDHRLRVSVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V222</accession>
    <name>A0A6E8V222_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="7">PID domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="EnsemblMetazoa" id="ACON000449-RA">
        <property type="protein sequence ID" value="ACON000449-PA"/>
        <property type="gene ID" value="ACON000449"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000799"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000449"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039613"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040369"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043197">
        <property type="term" value="C:dendritic spine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06720">
        <property type="entry name" value="PDZ1_APBA1_3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06793">
        <property type="entry name" value="PDZ2_APBA1_3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01208">
        <property type="entry name" value="PTB_X11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000007">
        <property type="entry name" value="Amyloid beta A4 protein-binding family A member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000017">
        <property type="entry name" value="Amyloid beta A4 protein-binding family A member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000207">
        <property type="entry name" value="Protein CBR-LIN-10, isoform a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051230">
        <property type="entry name" value="APP-Binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006020">
        <property type="entry name" value="PTB/PI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12345">
        <property type="entry name" value="SYNTENIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12345:SF16">
        <property type="entry name" value="X11L, ISOFORM F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00640">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00462">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01179">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PID" evidence="5">
        <location>
            <begin position="1624"/>
            <end position="1808"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="6">
        <location>
            <begin position="1843"/>
            <end position="1928"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="6">
        <location>
            <begin position="1934"/>
            <end position="2010"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="197"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="363"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="480"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="547"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="667"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="780"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="835"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1006"/>
            <end position="1087"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1158"/>
            <end position="1178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1191"/>
            <end position="1221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1369"/>
            <end position="1421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1434"/>
            <end position="1512"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1533"/>
            <end position="1615"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1728"/>
            <end position="1767"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="31"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="202"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="370"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="481"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="786"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1035"/>
            <end position="1051"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1063"/>
            <end position="1086"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1191"/>
            <end position="1211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1212"/>
            <end position="1221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="1380"/>
            <end position="1390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1391"/>
            <end position="1403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1434"/>
            <end position="1455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1557"/>
            <end position="1580"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000449-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="2024" mass="210205" checksum="DF174CA208514325" modified="2020-08-12" version="1">MVSSTVAFDRKPPAAAPVESAGAADRHDDSSPLSSTTSSSSLPFSGAAVETAGGGAVLTTGTDATTAAAAAGHELSLSEQKISLLFPKCKPRSDYHHYVLSSGASANGGGGGVGVGSGGGGKNSNNNCLMDVGSLSLGTYVTRTSPPTVATAQGAGPAKVYMDMGTFGNNNGGGGGSGGGGSGTGEFEYGAEKELVVGKGGSNNSNVTSNNNNNNNNNNNSSSTGNAGSGSAGGNNNHYGGNIKGELYGGIGGGSTGGSVKHGAPNQSAAKGGGSTVPDGYGYYYSNQPPPLTCSPTETQSAISAALGNGGGGGGGGPSTGLLLSDEDDTRDERLKREAAGAGPTPRWMERNYCHLSVSHYPSTGHKSKGGGPGVGGGSTGSGGGGGSMMQPPKRASVVGSGSTYKPVSCYNMVLQSPLEDESDMEDKLKYFNYQHEMQTYNGTRKDKLGLGGYGALGGDGGAAGEGEIVVLSSNAKEHGNTLSVAQPKQQDLPDEGPTARKGLLHLGQKLEHGPHVGLSVASPSVEQCEPAVDFADKLLGHYPAKRDEGEAELAENKSQSTGSNIIEYDGSPRRFGLNLCDEGDCYLASSVKINLTSPSLLSLPRPGFPQRVVPATVGTAGSGSSMAGTVAQTSSTNASCSPSLLDQVNSTLSKTLLLDSKRSANDTECTSSYGAGELSKVSGTRKEQQQQQHQQHHDHHIYDMLPGKLSPSEMGGKTSLEKLQLSAQTPENELQHIGGTSGFPPIANSSTFDYLYEFSETRKVLEEFFKCPSTEDVEKFSDYNDSAEEEDEEDGEEEDGESLDVHYDYPVELERSKTIETTYIGNVNGAAGKQDTLVRSPKSPLLGDGPARREEDDDTADDPLHYSLNGDGGDGRKVYARKYSKNFNYSPDTTDYDSNCGDYDSEISPQYGSDFGLISGNGATMCDDLNNGVISATSGAPSGPGESLNCLFANNANNYTRYYAAMPVLEDGLSSGHVSDSENNLSANPMTGLLPPDLVTVGSVKQHGQAGSNGGMMQSAGPATGPYGLLGAQSPSVTAKPSISSSPSMSKEMKAAQGSHVQQQQQQQQQQQQQHQPQQQQQQNQGTNVEGYFVNNTFRMGNLKLNSSMLHNASIFKNRDPELESLYTINTIQSTPPPPAPAPHRKSSIDCDNMSLLQQQQQQQQQPGQQQKQHQLQHLQQLQQQQQQSQQQQQQQGQPQQPQQSQQQQQFNHGQTTSADVQSTLKDIRLCLQKTKSLNLASSNQSRKAFDKGSLCATGDFPDPLGPVGSGTVGTAGSGGGAGTGPSLSPIWIPRTRASDDFAGKPLAVSTGPTAASGTGALSNGLQPGVGVSGPSCKLSSSPVCSKPVAVAGPDFADETDVLIGQGAGGPATKPLALVDEDEDPDTDLETDRLLGHQRLDDQGFYDESGSGKLWSTGERKTGASRLLTLGKVSPKQQQQQGASQPAGVAGGAARDTAANGKLPAAGPLGERLTGSVGVRNGPQPNGSAGGSSFLPGGGGSGSSTGMAGSGASVPLPDLGIAAAATLPPASGAPSTVNGGAGGTASAKSAKPPSGNLINFNGPNEPQTQLTSPNPSPQHSLGEGNLLDKSNDSGSPGGSTKSKSLHNNDSKRKSKNKEVLIEGVLFRARYLGSTQLVCEGQPTKSTRMLQAEEAVSRIKVGSGLPSPDGEVQPSTEVDLFISTEKIMVLNTDLKEIMMDHALRTISYIADIGDLVVLMARRRFVPQDVDSTDSSGGAGTGSGTHTAGHGAGSGSTPSQKLAGGPKGNRTPKMICHVFESEEAQFIAQSIGQAFQVAYMEFLKANGIEDHSFMKEMDYQEVLNSQEIFGDELEIFAKKELQKEVVVPKAKGEILGVVIVESGWGSMLPTVVIANLASAGAAARCGQLNIGDQIIAINGLSLVGLPLSTCQGYIKNTKNQTVVKFTVVPCAPVVEVKIKRPNTKYQLGFSVQNGVICSLLRGGIAERGGVRVGHRIIEINNQSVVAVPHEKIVNLLATSVGEILMKTMPTSMFRLLTGQENPIYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V231</accession>
    <name>A0A6E8V231_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Dynamin-type G domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8V231"/>
    <dbReference type="EnsemblMetazoa" id="ACON002456-RA">
        <property type="protein sequence ID" value="ACON002456-PA"/>
        <property type="gene ID" value="ACON002456"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002952"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002456"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030400"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09913">
        <property type="entry name" value="EHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.268.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031692">
        <property type="entry name" value="EHD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030381">
        <property type="entry name" value="G_DYNAMIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051943">
        <property type="entry name" value="TRAFAC_Dynamin-like_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43681:SF1">
        <property type="entry name" value="SARCALUMENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43681">
        <property type="entry name" value="TRANSMEMBRANE GTPASE FZO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16880">
        <property type="entry name" value="EHD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51718">
        <property type="entry name" value="G_DYNAMIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026312259" evidence="2">
        <location>
            <begin position="21"/>
            <end position="950"/>
        </location>
    </feature>
    <feature type="domain" description="Dynamin-type G" evidence="3">
        <location>
            <begin position="569"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="193"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="241"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="289"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="313"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="371"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="395"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="408"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="473"/>
            <end position="494"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002456-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="950" mass="105980" checksum="448AE9EC34F6FE97" modified="2020-08-12" version="1">MRRLSVWVLLGLALFTLSTAGLQVQAETPTEYECRPYIEKAIQDLKSESAEAADEENASIEHTIDVTSQEEEDNTAAEEVSTEETDTAADDATEEITPSEEVQEHLFADTTEDEATDNVEASEETDQEQAEAADSEEGEEEQAEDQEENVTEEAVQSEETEPTVSEEAEQSEEVLAETEELTSEESANQVLEDATEEEEDEEAAAEDDGEQDEEEDVATEEVTESAEEEATDTDQTKSEESVVDDDGEEETEENDDDDEEETQAQSSSEDEEDDDLDEENAAKGSYDDREQEDVEDASAEANEASEEVAVSGEETESEDEPASQEKADEDAADDENLSEETDNETVDETATSEEENAEAEPASDEQLAVDSQEEQEDAAQDEPSTEEPAEESSEDATAAAEEGSQEQQEADSAEEATDSQQAAEDSSAEESVTESTEQEAGVPAEGESEEETVVKQDSEESLPADGEASVEQSAEESETASQEEEKEESFPEEDLLLEYEIPTNLRDTTHVYELLNINDDASSREKALQETADVILRDLKRIYDNSIKPLETLYKYRDLSNRHFGDPEIFSKPLVLFMGPWSGGKSTILNYLTHNEYTPNSVRTGAEPSPAYFNILMHGDEPEVLDGTQLAADWTFSGLQKFGQGLLDRLRGQKLPNKLLERVNIVEIPGILEVRKQVSKYFPFNDACQWFIDRADIIFLVYDPSKLDVGPETEAILDQLKGREYQTRILLNKADQVKPEELLRVQSALIWNISPLMSSAQPPVMYTVSLWSNPYEVGAPVRLLQAQERSLLLDLGQAIDRRIENKIASARRFAVRVRNHAKMVDCYLTTYYNHKTLFGNKKQTAEKIIASPQDYHIYEGLSTLTNISRYDLPDPEVYRDFFHLNPLYEFKKLSETCTYFRGCPINKLDVAIAYDLPELVGKYKKMAEAALAKLDVLTPTSDFGKGKSNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V232</accession>
    <name>A0A6E8V232_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Neuropathy target esterase sws</fullName>
            <ecNumber evidence="4">3.1.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Swiss cheese</fullName>
        </alternativeName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="23">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Phospholipase B that deacylates intracellular phosphatidylcholine (PtdCho), generating glycerophosphocholine (GroPtdCho). This deacylation occurs at both sn-2 and sn-1 positions of PtdCho. Its specific chemical modification by certain organophosphorus (OP) compounds leads to distal axonopathy. Plays a role in the signaling mechanism between neurons and glia that regulates glia wrapping during development of the adult brain. Essential for membrane lipid homeostasis and cell survival in both neurons and glia of the adult brain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a 1-acyl-sn-glycero-3-phosphocholine + H2O = sn-glycerol 3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15177"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.5"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Interacts with Pka-C3; interaction inhibits the catalytic function of Pka-C3 and the esterase activity of sws.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NTE family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.5" evidence="4"/>
    <dbReference type="RefSeq" id="XP_040235947.1">
        <property type="nucleotide sequence ID" value="XM_040380013.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8V232"/>
    <dbReference type="EnsemblMetazoa" id="ACON000467-RA">
        <property type="protein sequence ID" value="ACON000467-PA"/>
        <property type="gene ID" value="ACON000467"/>
    </dbReference>
    <dbReference type="GeneID" id="120957683"/>
    <dbReference type="CTD" id="31716"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004359"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000467"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030210"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004622">
        <property type="term" value="F:phosphatidylcholine lysophospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046470">
        <property type="term" value="P:phosphatidylcholine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd07225">
        <property type="entry name" value="Pat_PNPLA6_PNPLA7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000010">
        <property type="entry name" value="neuropathy target esterase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1090.10:FF:000001">
        <property type="entry name" value="neuropathy target esterase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000135">
        <property type="entry name" value="Neuropathy target esterase sws"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001423">
        <property type="entry name" value="LysoPLipase_patatin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050301">
        <property type="entry name" value="NTE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056556">
        <property type="entry name" value="NTE1_P-loop_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14226:SF29">
        <property type="entry name" value="NEUROPATHY TARGET ESTERASE SWS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14226">
        <property type="entry name" value="NEUROPATHY TARGET ESTERASE/SWISS CHEESE D.MELANOGASTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24179">
        <property type="entry name" value="NTE_Ploop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01237">
        <property type="entry name" value="UPF0028"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 18" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11 18" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="13 18" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="32"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="21">
        <location>
            <begin position="162"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="21">
        <location>
            <begin position="541"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="domain" description="PNPLA" evidence="22">
        <location>
            <begin position="871"/>
            <end position="1037"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="322"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1231"/>
            <end position="1280"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXGXXG" evidence="18">
        <location>
            <begin position="875"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="18">
        <location>
            <begin position="902"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DGA/G" evidence="18">
        <location>
            <begin position="1024"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="1262"/>
            <end position="1271"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="18">
        <location>
            <position position="904"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="18">
        <location>
            <position position="1024"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019369"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00025020"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00030862"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00049531"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000467-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1280" mass="143358" checksum="A198CEE322410710" modified="2020-08-12" version="1">MDIAGLVKKSYADYGTMLKDSWYTINTEDLHLSWAIKLLVALFAIIATVYVWRRKHLRKLEEKMQLQHQYASHMRFRKRDKMMFYGRRMLRKVKSISGQAYGGQGRKRRAVMRFAKRLLLRKDVTPTQLLVIEPPAEYLEVASDGNDRVPPDALYMLQSIRIFGHFEKPVFLKLCKHTEIINLIAGESLIKVGDPDDSVFIVQTGQVNVFLNNPDGSSITLKVVRQGESVTSLLSFIDVLVGNASTYKTVTARAMENSQVIRLPMFAFQQVFGECPDLLVRVIQVIMVRLQRVTITALHNYLGLSTEYIQCSSQRKKLPIAPATKGSPGHRRVPSDQVHPVPHSLVGEIRPDMLVDLSEGTHNVGRRTSALPIEPIDHAVLKAHAVDGFMQELDLPDTFKQTIEESILIKEVSPGTTLVHQGVLDDVMLIFVIAGGLTLTQCPQSGPIAQDASKAGGEKMDNHTVTIYPGDVVGGLAVLTGECSLYTIRAKHNSRVGLLNKDVIYRIMRERPAVVLDIANSVVKRLSPLVRQCDFALDWNFIESGRAVYRQEETSDSTYIVLNGRLRSVTTHANGKKEIVGEYGKGDLIGIIEMITETPRTTTVMAVRDSELAKLPEGLFNAIKFKYPIVVTQLISLLSHRILGSMRSRPISTSSFTSVSFDTNQQIQHRYSTVAIVAVSDDVPLSPFTYELYHSLSTIGSTVRLTSEIVRKALGAAIMEPPNEYRLTSWLGQQEDRHNITLYQCDNSFGLWTQRCLRQADVILIVGFGDRSPVMGKLEKEIDRLAIRTAKELVLLHPEEASSCPSNTIGWLNMRAWVSRHHHIQCPKRMFTRRSQNKIAELYERIMKNEPYIHCDFSRLARWLTGNSVGLVLGGGGARGAAHVGMLKAIQEAGIPIDMVGGVSIGAFMGALWCMEKNITTMTQKAREWCTKMTQWSRQLRDLTYPITSMFSGRHFNQTIRGTFGDICIEDLWIPYFTLTTDISASCARVHTHGSTWRYVRSSMSLSGYMPPLCDPTDGHLLLDGGYVNNLPADVMRAQGAAHIIAIDVGSQDDTDLTDYGDDLSGWWLLYKRWNPFTSPVKVPNLPDIQSRLAYVSCVRQLEEVKKSDYCEYIRPPIDQYKTLAFGSFDEIKNVGYEHGKAYFQSMAESGRLTRFNQWSVHPIPKKATHSLNEYSFVDLAQIVCKVPETYPERGYSSSEDDYYDGYASEPSSMPLKKGYNMPLMRSAGSLSLSDNDMDSDELEIPRPFKPLPGNRNLQAVAATAAGTDGSSGDGKKPLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V253</accession>
    <name>A0A6E8V253_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">NADPH--hemoprotein reductase</fullName>
            <ecNumber evidence="8">1.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="1.6.2.4" evidence="8"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8V253"/>
    <dbReference type="EnsemblMetazoa" id="ACON000500-RD">
        <property type="protein sequence ID" value="ACON000500-PD"/>
        <property type="gene ID" value="ACON000500"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004146"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000500"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042582"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003958">
        <property type="term" value="F:NADPH-hemoprotein reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009725">
        <property type="term" value="P:response to hormone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06204">
        <property type="entry name" value="CYPOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.990.10:FF:000001">
        <property type="entry name" value="NADPH--cytochrome P450 reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.80:FF:000001">
        <property type="entry name" value="NADPH--cytochrome P450 reductase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.990.10">
        <property type="entry name" value="NADPH-cytochrome p450 Reductase, Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003097">
        <property type="entry name" value="CysJ-like_FAD-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001709">
        <property type="entry name" value="Flavoprot_Pyr_Nucl_cyt_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023173">
        <property type="entry name" value="NADPH_Cyt_P450_Rdtase_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19384:SF17">
        <property type="entry name" value="NADPH--CYTOCHROME P450 REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19384">
        <property type="entry name" value="NITRIC OXIDE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00667">
        <property type="entry name" value="FAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00371">
        <property type="entry name" value="FPNCR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0288">FMN</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding FR-type" evidence="9">
        <location>
            <begin position="73"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000500-PD"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="469" mass="53617" checksum="9FE06C8E9F854809" modified="2020-08-12" version="1">MSVLAGGARQSCSRLIALNSIHQQYLAPIVVQCLPRIQQLLLDHRGKFRRKYLQVFGLGNKTYEHYNKVGIYVDKRLEELGANRLHKAGGRSCMHVEFDIEGSKMRYEAGDHLAMYPVNDRDLVERLGRLCNAELDTVFSLINTDTDSSKKHPFPCPTTYRTALTHYLEITALPRTHILKELAEYCGEEKDKEFLRFISSTAPDGKAKYQEWVQDSCRNIVHVLEDIPSCHPPIDHVCELLPRLQPRYYSISSSSKLHPTTVHVTAVLVKYETKTGRLNKGVATTFLAEKHPNDGEPAPRVPIFIRKSQFRLPPKPETPVIMVGPGTGLAPFRGFIQERDHCKQEGKEIGQTTLYFGCRKRSEDYIYEDELEDYSKRGIINLRVAFSRDQEKKVYVTHLLEQDSDLIWSVIGENKGHFYICGDAKNMATDVRNILLKVIRSKGGLSETEAQQYIKKMEAQKRYSADVWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V5Y2</accession>
    <name>A0A6E8V5Y2_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Fatty acid desaturase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the fatty acid desaturase type 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8V5Y2"/>
    <dbReference type="EnsemblMetazoa" id="ACON003049-RC">
        <property type="protein sequence ID" value="ACON003049-PC"/>
        <property type="gene ID" value="ACON003049"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000039"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003049"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031541"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004768">
        <property type="term" value="F:stearoyl-CoA 9-desaturase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006636">
        <property type="term" value="P:unsaturated fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03505">
        <property type="entry name" value="Delta9-FADS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015876">
        <property type="entry name" value="Acyl-CoA_DS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11351">
        <property type="entry name" value="ACYL-COA DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11351:SF21">
        <property type="entry name" value="GH07782P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="103"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="134"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="166"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003049-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="289" mass="33197" checksum="8287BF52B8723938" modified="2020-08-12" version="1">MTVGITSTLQFIAGNSAQYMKPGPNGQSQQAAAAAAAAAASSTVPDTNNNEMEDDRTVSDKDIDSYLKHYNRWQQSAWGRTLSDLVKRYLGYEFEDEVKWNNVFMIGIFHVIAVWSFLHYVWQATPITYLWNFVLMKLLFCFILPTFIPWYFFGETFLMSFFSQCFVRYVLSLNFTWLVNSAAHLYGNHPYDKRINPAENRAVSVVAMGEGWHNYHHVFPWDYKAAELGNYSVNVTTFWLDVFAKIGWAYDLKEPSKDLVRRTIEKYGDGTHITARIGHMEEVPAPADS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V5Z3</accession>
    <name>A0A6E8V5Z3_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">glutamyl aminopeptidase</fullName>
            <ecNumber evidence="6">3.4.11.7</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="31 32">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="31" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="31">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of N-terminal glutamate (and to a lesser extent aspartate) from a peptide.</text>
            <dbReference type="EC" id="3.4.11.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="24">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="24">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer; disulfide-linked.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Lipid-anchor</topology>
            <topology evidence="3">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase M1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.7" evidence="6"/>
    <dbReference type="RefSeq" id="XP_040229268.2">
        <property type="nucleotide sequence ID" value="XM_040373334.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8V5Z3"/>
    <dbReference type="EnsemblMetazoa" id="ACON003077-RA">
        <property type="protein sequence ID" value="ACON003077-PA"/>
        <property type="gene ID" value="ACON003077"/>
    </dbReference>
    <dbReference type="GeneID" id="120953428"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005492"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003077"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033592"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004230">
        <property type="term" value="F:glutamyl aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09601">
        <property type="entry name" value="M1_APN-Q_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.50.20:FF:000001">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1910:FF:000003">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1730:FF:000001">
        <property type="entry name" value="Leucyl-cystinyl aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.390.10:FF:000006">
        <property type="entry name" value="Puromycin-sensitive aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024571">
        <property type="entry name" value="ERAP1-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034016">
        <property type="entry name" value="M1_APN-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001930">
        <property type="entry name" value="Peptidase_M1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050344">
        <property type="entry name" value="Peptidase_M1_aminopeptidases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533:SF276">
        <property type="entry name" value="GLUTAMYL AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533">
        <property type="entry name" value="PROTEASE M1 ZINC METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11838">
        <property type="entry name" value="ERAP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00756">
        <property type="entry name" value="ALADIPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="15" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="20" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="21" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="22" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="19 27" id="KW-0472">Membrane</keyword>
    <keyword evidence="12 24" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="10" id="KW-0645">Protease</keyword>
    <keyword evidence="32" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="11 27" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="17 27" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="14 24" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="27">
        <location>
            <begin position="18"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="domain" description="Aminopeptidase N-like N-terminal" evidence="30">
        <location>
            <begin position="127"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M1 membrane alanine aminopeptidase" evidence="28">
        <location>
            <begin position="355"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="domain" description="ERAP1-like C-terminal" evidence="29">
        <location>
            <begin position="658"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="59"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="26">
        <location>
            <begin position="65"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="23">
        <location>
            <position position="428"/>
        </location>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="427"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="431"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="450"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="25">
        <location>
            <position position="513"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="Pfam" id="PF01433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="Pfam" id="PF11838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="Pfam" id="PF17900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003077-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1001" mass="112099" checksum="2D43C644991571B9" modified="2020-08-12" version="1">MVNQLLQQSKDWVLRNKVATFLSVALFAMVITTIALAVSNNSNASDLEECEVQLAPFLSTTNVPTTESSDATTSDQQTTTEGTGGATTTTVSMDTSPLPEVTTVTSVSTTTTVPGVINYRLPDDTIPLHYDLLLHPDLEKNTFAGTAKITIWSVRATDQVVLHSHELLAIGRITFKCLNDSTYTYINTNVDRERDFLKINLNKALLANYISELTIDFTGRLDAGIVGFYSSSYSDGSGGTKTIATSKFEPTFARQAFPCFDEPHLKAEYTIHMVHPSGDGYAALSNMNVKETVANKPSAGLSTTTFERSVSMSTYLVVFIVSDFLHQEVLILPEHGSSFPLRVYATPFQQKNTAYALATARTIIEYYVKYFGIAYPLPKLDMAAIPDFVSGAMETWGLVTYRETSILYNSETSSTANKQRVAGVIAHELAHMWFGNLVTMKWWNELWLNEGFASYIEYKGMDAAHPDWGIEEQFIIDDLHGVLNLDATLGSHPIVMSVENPNQITEIFDTITYSKGASVIRMLEDFVTPPVFQQGVKRYLEKLAYANSVSEDLMRELDELVPDVSVTDVMDTFTRQKGLPVVTVAENALQYVLRQQRFLADQDANETEESPYGYRWYIPITYLASTDDPATAAPRRIWFPNDASRPELVIDKPAGSSWIKLNYRQIGYYRVNYPIAMWQQFGEALRKEVNTFTIGDRTGLLNDAFALADASLLAYNHALELTRYLSGETEYVPWSAIASKLKNIRNLLYNYQSYDDITTYTQTLVDAAVKSVGWEVPAEGGHMTNLLRTTILDLACSFGHSACLEEASKQFRGWLNAGAVIHPDLRSVVYTYGIQSGVTVADWDKVLERFRQENDANEKTKLMVALASYPDQRTMRRFLDLSWDTALVRTQDQLSCIQYIAANRAGEQAAWEHVRENWPRLVARFGIGERNLGRMIPSVTGRFTTQARLTELEDFFARYPESGAGATARRQALENIQNNISWLERNEANVAAWLKEATAPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VA03</accession>
    <name>A0A6E8VA03_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="13">G_PROTEIN_RECEP_F1_2 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VA03"/>
    <dbReference type="EnsemblMetazoa" id="ACON001499-RB">
        <property type="protein sequence ID" value="ACON001499-PB"/>
        <property type="gene ID" value="ACON001499"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_008828"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001499"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_036120"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24228">
        <property type="entry name" value="B2 BRADYKININ RECEPTOR/ANGIOTENSIN II RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24228:SF63">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR MOODY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="200"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="304"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="333"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="203"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001499-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="398" mass="44419" checksum="5C4D62CFD441A7F3" modified="2020-08-12" version="1">MEQRYFRSANDRHGDGRGGDRLPGRNQQIFPPIANVCCRRDDHNYGHGNCGQFAYDCRTAQMSQGAKCGGRLHYQVSPVHSGLSVLHHRPAIQRDALHPGHVDARRDALHADNVYAIRQRRRVAALHHDDQHKPLHYDRTLQHLPEGVQDVLDYGDDRCLLAVFLRLPTANAFRWKFGYNHQLGTCSILPDDEGRSSKTAMFIIAFIIPCMIIVICYSRIFCVVHKSDKRMKNHSKSQCNIPNNLRSAADPASPLSAGSSSSANNNSISYTASSSAVKVAGAARTAADARDAKARRNEWRITKMVLAIFLSFVMCYLPITISKIVDKNVSVPVLHIIGYIMLYLSACINPIIYVIMNKQYRQAYKTVIFCKPSRLLGFGGSSVGDSPHPQHLVAIQDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VA09</accession>
    <name>A0A6E8VA09_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">MAP7 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MAP7 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VA09"/>
    <dbReference type="EnsemblMetazoa" id="ACON001315-RB">
        <property type="protein sequence ID" value="ACON001315-PB"/>
        <property type="gene ID" value="ACON001315"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000686"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001315"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034867"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015630">
        <property type="term" value="C:microtubule cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051483">
        <property type="entry name" value="MAP7_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008604">
        <property type="entry name" value="MAP7_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15073:SF18">
        <property type="entry name" value="ENSCONSIN, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15073">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05672">
        <property type="entry name" value="MAP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="55"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="106"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="153"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="217"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="330"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="426"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="454"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="515"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="857"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="886"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="987"/>
            <end position="1031"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1058"/>
            <end position="1115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="57"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="107"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="153"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="219"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="330"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="364"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="383"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="405"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="435"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="454"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="469"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="521"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="585"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="656"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="886"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="987"/>
            <end position="1028"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1058"/>
            <end position="1069"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1076"/>
            <end position="1100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1104"/>
            <end position="1115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001315-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1150" mass="127192" checksum="818C90B4FFF7CDBE" modified="2020-08-12" version="1">MLTDESDRIPSTTLAVLRGILVKSLSRENLSLTDQQQLLGTRKARHVQFSLAGNRDATVQKQSRPSSANRELQKEREERIKQMKERQNEERQKKLEELKAQALAAQKFREQKEEERRRRMDDLRRRESDRRSQVEERRRAIIEADNERREYILRKNQERDQRMETKRRNDRGSIQFAFGSSTPRMIDTSDSGMCSSFWAHRRATSITNVAYTGAALTRRSSERELTDSASKKRATSASGLDRSTDDDTAEMNYQRTVNRRKTDLMPTIPSPRDSSRSSLGTHTPRTPGRAFSMTRLDQLAQPRRRNGEHISAILERERRQALELENLTRLSLSSSRSSPTGSGGNSKRMSRSMSQLAGSGAKYRNQSQDSNSGRSPGFGGGRKSSFNSSSINSGSTSPMRRNDTSKSMSQLNTAGSTPRITKTERLRQQYHHHQQQQQHHQQHQNQLLVTNGLRSGEMTPTSLNTSRPGSAMSSSTTASGIVYRRTLPAQRKPRPASIAVTGVSASGLKEGFATSKHRLSSAGSGSITTTPAKSASGTETPTKKPLTLHSAEKRSAGARGTTPKASSTPLQSPGPEKASRNLLDSLKKSADGPKKAPTPKTPAQKDDAVVVEAKQEPPSVAAPKVEELSAQQEAILQRDDGSQQASLVEVDQSFGQEEQPQQQQEEVVVPVQQEAPAPAQTEDAELPKQAEEAVTVEVVEQAPITKQQQQAPLEQQDVSAVQAAEPTVMVAEEVPENVNGSLTVEVSGAGADAMTASMIAKRINTEEEAKAALAERRRLAREEAERLAELERKRIEAEEQAERQRILEEEERLRKLEEETIRLAEEQRRMEEERLQQAIEEARRRDEEERKRREEEARQKAEREEAERKAREEAERQRVEMAERLKKEEKEREERRKRVEAIMSRTRAKGAANNTPTKNNEDDKENAMSKSQIVLSSTPAALPTGTAMSASTISMTDSFIASEIRQEQQQQQQQTQHQSLEQAMESLSLSNNNTTSSNSSSSSSDTLANSSTINNNINNNNNNTNGGNVQFDKSVTEKENLLLASSTAGAAVVVANNNLNNGSTSNGTVVEPATNEEGTNGTKGESLLLTTNGKSEQQSPAMMASSNGSSNATTANSTSDLIIEDALMGQTNGHKNGTMDNVLFPRVSDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VBP3</accession>
    <name>A0A6E8VBP3_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">MICOS complex subunit MIC60</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Mitofilin</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the mitochondrial contact site and cristae organizing system (MICOS) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Mitochondrion inner membrane</location>
            <topology evidence="7">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the MICOS complex subunit Mic60 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_049462965.1">
        <property type="nucleotide sequence ID" value="XM_049607008.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VBP3"/>
    <dbReference type="EnsemblMetazoa" id="ACON002364-RA">
        <property type="protein sequence ID" value="ACON002364-PA"/>
        <property type="gene ID" value="ACON002364"/>
    </dbReference>
    <dbReference type="GeneID" id="120952310"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_013088"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002364"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_036331"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061617">
        <property type="term" value="C:MICOS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042407">
        <property type="term" value="P:cristae formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019133">
        <property type="entry name" value="MIC60"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15415:SF7">
        <property type="entry name" value="MICOS COMPLEX SUBUNIT MIC60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15415">
        <property type="entry name" value="MITOFILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09731">
        <property type="entry name" value="Mitofilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="119"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="196"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="285"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="427"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="125"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="149"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="165"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="196"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002364-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="739" mass="82079" checksum="D106F597953797F2" modified="2020-08-12" version="1">MYRLLVQKALANNSKRPSAQLFAASRSYSGSARGSNLPPLQEAGFGKVLVVLSPILIGGGVVTYAKYDNEFRKTLITNVPALEPVLKTLLQETNPLDEVSKKMDDISKTIGEYTSTITGFFGGGEEEKKQEKKPDLPPITRAKSVHVPVPSPPLPKPEPINLSDKVPEQPKKKPEAATPKVTTATASKAAAAATAAAPAKSAPAAPSKPVPLASGGETVPKSISDLEQQVEVAATIAIKEYGHAVDVLKSYTNEVRKVVDESVDKLDSSSWTTLRNRTSARDTALQAAEDAADKARGNIEKLHALLNSREIKCSDELKDKARQNIAAYLDHLKKAKEEVYAARDLASLGEKYWKRVESARNYFVEEMESLFPGINLAERKLNLSKDELDLFILHAYTQVIAHQKELQKLQIEGDQNLRRALEAVRGSDQSEEVKARLEYEIAKEKRQLNLLNQKKLLLTRAELEQQMREQLKRQTEAHVDHLKDALTQKELEMKRKFQRELDEKITTEQASYKLQLAAMLGKLKGIHNALVEHADAEKSAHQAQALWGACQSLWSSIRSGQPGKSWRDQLRPLKDEIAAVARSAEGDELVTVVLKGLPETAVKRGVYPEDALRERFLKVEEVSRRLALVPAEGARLPMYVLSYLQAALIARPDKPISQDELENKPFDFSKLDTYDILNRARYWLDRGDLVKTVQYVNLLQGAPRKAALDWLNEARLLLETQQAASTLMAHAAASGVRFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VBQ8</accession>
    <name>A0A6E8VBQ8_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">uS12 prolyl 3-hydroxylase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>[ribosomal protein uS12]-L-proline + 2-oxoglutarate + O2 = [ribosomal protein uS12]-(3S)-3-hydroxy-L-proline + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:54156"/>
            <dbReference type="Rhea" id="RHEA-COMP:13816"/>
            <dbReference type="Rhea" id="RHEA-COMP:13818"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:50342"/>
            <dbReference type="ChEBI" id="CHEBI:85428"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TPA1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040222979.2">
        <property type="nucleotide sequence ID" value="XM_040367045.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VBQ8"/>
    <dbReference type="EnsemblMetazoa" id="ACON002933-RB">
        <property type="protein sequence ID" value="ACON002933-PB"/>
        <property type="gene ID" value="ACON002933"/>
    </dbReference>
    <dbReference type="GeneID" id="120949627"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012080"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002933"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_036944"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031418">
        <property type="term" value="F:L-ascorbic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031543">
        <property type="term" value="F:peptidyl-proline dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006449">
        <property type="term" value="P:regulation of translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019601">
        <property type="entry name" value="Oxoglutarate/Fe-dep_Oase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006620">
        <property type="entry name" value="Pro_4_hyd_alph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039558">
        <property type="entry name" value="TPA1/OFD1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051842">
        <property type="entry name" value="uS12_prolyl_hydroxylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12117">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE COMPLEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12117:SF0">
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<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Anopheles coluzzii</name>
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            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
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        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
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                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
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        <scope>IDENTIFICATION</scope>
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        </source>
    </evidence>
    <sequence length="1478" mass="154858" checksum="B186DBECFA355A8F" modified="2020-08-12" version="1">MYKREQQISADAPLQLDRNNNSASGKRNTSNGDRPPASTTTGGAFQRSGSTLRRNVERKRFFAAAAATTATPVLPAVAVAVVPIDTDGPVQTVDKNVATEQQQQQQQTESSGGGEQQQRANMLLTSHDHVGFKMMGFIKEGEIYATSIGNDGGGVGDGESDQRGSATRVGTAGRGRKTVIPEINVIAAPPTTTTDLEQDGKTINHSASPFRRWGQSSLRSRSTEHRTPTNGSARRPSSLAASESDVYTKSIDDDYSSTKSGYNPYTGGGSAGGGVDTLSAESATIAAGPMGTAGTGGSGGTTTGPTGVASWGTSFEKLLEDAAGLHTFSEFLKKEFSAENIYFWTACERYRQLTEREERAREAQAIFARHLESGCSEPVNVDSIARNIALENLPQAEPTLFAAAQKQIFNLMKFDSYQRFIKSDLYRVCQEAEGKGQGLPYPGEQLDPMLRTSATMLTASGTGTAASITKLKKSLSNAEDRRRKSLLPWHRKTRCKSKDRGDSDTSKKDSKEKGGGSGGGGGGEKASGTTATGSSSNTLKLLSTNSTSDIHSSRSSLASFDAAIGGKSCDPDDSRNTLCRVILSNGATTVVQTRSNETIKELVERLLEKRGIVYNAYEAFLAGSTKPLDLDGPSVSLAGKEVNIDQRVVFKLNLPNRKMISVKSKAAKPLADVLRPILHKYNYELDEMKVVVHSTVDVCLDMTQPVTTVDGLCLYIRSANETPNVHPELGRPLAGARPVINTATHQQIVHHFQQQQQQQLLQLQNGAQASAAGQQQHTFAVPSALPPSYAVATNSKSQQLLGNGTAASATCNNNTVNQTQSRAKEQSEQQSKDDQQQQQQAGVGAGTRGQQELNSLDEITNKVFDELRNGKTAFGGVVTAVGKGPGTVDVDCASDTSSTRRDRFRRRGSNAAPSESGRGAKSKKCSTGGSEDGGESVNNLGIKKPIIAKLKAGVKLQIPTRSQNDELLEGLKRAQRSRLEDQRGTEINFELPDFLKDKENFSSSSAAVSSSAGSIGASAAPSSAAGPSKLTRSKPARRSEATTPTAEFVGGSLQQINKPQPAPRLSITGQQGRQNLSGPGSPVHAAVSDSHLNLSTQCNASPLPQDDPFHVHGGGSGTNGGSQSEISYADTTLVFTHARPYNGQALVTATPRTIPSSSASQHYSGDHSSDSSSTADGPDLPNLACCEHHPHEMSPDSSSPPTHANHLHPLHYHPAGAGASGANGAAGATPNGNTSTNNPYPLAVHYPSHHHHHPHLHPHHHPHGQHYHHHHHHHTHNGGGGYQTATSQPVANGSNAPGQDGQPNATSGGAPDSQKGPPPLPPKPKILPIKPSNWGHPVAASYAPGPPGSAGAPGGGTSELLGGLSTTTLSTNGSMGGSVNGTGATVGGLSPSSSTVSSHHPNSNSNGVGVGTVVGSSNGIVSSTTHNDLSRGGREGNRQQRASSGNDGGSSNGSASAISVAVAQARNVYFDQGNSSFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VBS3</accession>
    <name>A0A6E8VBS3_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Importin N-terminal domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VBS3"/>
    <dbReference type="EnsemblMetazoa" id="ACON002970-RB">
        <property type="protein sequence ID" value="ACON002970-PB"/>
        <property type="gene ID" value="ACON002970"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_008305"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002970"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032671"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058669">
        <property type="entry name" value="TPR_IPO7/11-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997:SF7">
        <property type="entry name" value="IMPORTIN-11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997">
        <property type="entry name" value="IMPORTIN-7, 8, 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25758">
        <property type="entry name" value="TPR_IPO11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Importin-7/11-like TPR repeats" evidence="1">
        <location>
            <begin position="79"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF25758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002970-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="444" mass="50340" checksum="D1E3D5A98B6C8988" modified="2020-08-12" version="1">MDDFEFVAEQFVEFLEPAVALLFGLLKEAVECETKMTVLYVMSFIIEKMSMSMRIDVQSLVQYLPLLWEESREHNMLRCAIISTLLQIIKALYEIPSSEPIVAFIYQIIEMSTNVNDPSHVYLLEEGLELWVVVVHYSRTMNQELLNLCENLVPLIQQSSSNMNICLAIVQAYVFLGAEVFLPRYGQEIVKTCQYLLTDLRADGVVLINRFFLTLLQAVPKFAIELLRPSYYQQTNFPQVLQIYLQIISRVLVNDQVTFSVVLAETGAQDALEKILTAWLENMRRVTAIEERKLLALALSSLLTVSNDVIYKNFAGIITNVTEALNDIMDVFSQDTKVDSLVIDDENVDNVGVTLFSYGFIDSDMVQEETPHFSRCRAFCLRDPTHVIVLKDYLQNQLVVLKTTIGAEQYQSLMTSVDLQTLKELSSFVALGIDLPTGIDDGAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VBU3</accession>
    <name>A0A6E8VBU3_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein UBASH3A homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VBU3"/>
    <dbReference type="EnsemblMetazoa" id="ACON002463-RA">
        <property type="protein sequence ID" value="ACON002463-PA"/>
        <property type="gene ID" value="ACON002463"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007991"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002463"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035785"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003993">
        <property type="term" value="F:acid phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1240:FF:000032">
        <property type="entry name" value="Blast:Protein UBASH3A homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051710">
        <property type="entry name" value="Phosphatase_SH3-domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16469">
        <property type="entry name" value="UBIQUITIN-ASSOCIATED AND SH3 DOMAIN-CONTAINING BA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16469:SF27">
        <property type="entry name" value="UBIQUITIN-ASSOCIATED AND SH3 DOMAIN-CONTAINING BA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00083868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002463-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="320" mass="35847" checksum="C1014EE96730A249" modified="2020-08-12" version="1">MFGGNKTMDDIPLEKLHELVKKRTNMQVIPGDQCASQSQSVPRTESGTRKVYVMRHGERIDFTFGSWVPYCFDEAGNYVRKDLNMPTSLPTRKPSLWQKDSPLTNVGRYQARLVGEGLKDAGVQIARAYCSPSFRCIQTATSVLEGLGLKATVPICIEPGLFEWLAWYQDGLPEWLTPDELLAADYNIDAAYKPLSSPGLLREHFHEKLNEFYCRNSNAAEEIIKSTEGNVLIVGHATTLDTCTRFIIGEKLRSTNDMARIMQKVSYCSLAVMESDGTTPSGDSEQAESTVKGHWKLAVPPCDPVTHTNNNRFDYKILLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VC44</accession>
    <name>A0A6E8VC44_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">CHK kinase-like domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VC44"/>
    <dbReference type="EnsemblMetazoa" id="ACON003220-RA">
        <property type="protein sequence ID" value="ACON003220-PA"/>
        <property type="gene ID" value="ACON003220"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007619"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003220"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031355"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015897">
        <property type="entry name" value="CHK_kinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004119">
        <property type="entry name" value="EcKL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11012:SF57">
        <property type="entry name" value="LD10016P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11012">
        <property type="entry name" value="PROTEIN KINASE-LIKE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02958">
        <property type="entry name" value="EcKL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00587">
        <property type="entry name" value="CHK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CHK kinase-like" evidence="1">
        <location>
            <begin position="142"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003220-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="424" mass="48818" checksum="CEB4D8ECD970F4FD" modified="2020-08-12" version="1">MDTQRVALKLSDISPKFTEETLDEIVRCAGGKRCTGWKIPETNFTKGDAYLSELYRIQLTGEPAEPGRDEPLVVNVVVKTIPKNVGRRNTFRSADFFRNEANFYNVVLKELYRFQDARKPANPFKDINPCYVAYTDGVNDFVAMEDLGQYGYKTASRAEGVGLEECKRCMRSLGRFHALSLAMKEQEPDRFHEIAQQHVEETYYSARLKSWYNNFLQVQIDIARDAMAREYPGTELERTMEKFFDCDLYDHMVYLTHTRNQNSVINHGDCWMPNFLFHDSTPAMRMIDFQLARYCSPALDIAFFVYSCTSQALRDAHYEDLLGAYHGGLAEMLRDLGSDPDTVFPRAELEKEMRQYARFGCGMGIESIPFSLLDESEVPDLDKITGEQAVPIEQLWILRPIASQAGRRRLTDMFRHATDMGYLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VC65</accession>
    <name>A0A6E8VC65_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Guanylate kinase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MAGUK family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VC65"/>
    <dbReference type="EnsemblMetazoa" id="ACON002711-RB">
        <property type="protein sequence ID" value="ACON002711-PB"/>
        <property type="gene ID" value="ACON002711"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000007"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002711"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032683"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030054">
        <property type="term" value="C:cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00071">
        <property type="entry name" value="GMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06798">
        <property type="entry name" value="PDZ_MPP5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12036">
        <property type="entry name" value="SH3_MPP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000088">
        <property type="entry name" value="MAGUK p55 subfamily member 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000469">
        <property type="entry name" value="MAGUK p55 subfamily member 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.460">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
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        <property type="entry name" value="Guanylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020590">
        <property type="entry name" value="Guanylate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014775">
        <property type="entry name" value="L27_C"/>
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        <property type="entry name" value="L27_dom"/>
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        <property type="entry name" value="MAGUK"/>
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        <property type="entry name" value="MPP5_SH3"/>
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    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
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        <property type="entry name" value="PDZ"/>
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        <property type="entry name" value="PDZ_sf"/>
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        <property type="entry name" value="SH3-like_dom_sf"/>
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        <property type="entry name" value="SH3_domain"/>
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        <property type="entry name" value="MEMBRANE-ASSOCIATED GUANYLATE KINASE MAGUK"/>
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    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="GUANYLATE_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50052">
        <property type="entry name" value="GUANYLATE_KINASE_2"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="L27" evidence="9">
        <location>
            <begin position="287"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="8">
        <location>
            <begin position="432"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="6">
        <location>
            <begin position="524"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate kinase-like" evidence="7">
        <location>
            <begin position="674"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="64"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="77"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="107"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50052"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS51022"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002711-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="870" mass="95653" checksum="9B816858AD20C207" modified="2020-08-12" version="1">MDTGRPRRRSGSSIVVLGGDDTLKPSIPIDDYQEDLEMYNMLAANQDNGPHREMAVDVPESFIARNKTPPRYPPPRSTVTAASAAASTPATPASTQLNGSSKPVPPPRDHLRIEKDGRLTNRAPVPAPQVPDRKIVPNASSQHQHIGQVLEPTPDQLDSIKKFQEQLRRRREDEERIAAQNDFLRNSLRGSQRLRALQDNPIEKPPVGVDNEAYADDEVVAVAAEKIIGYGELVAALQRLQGHLNKHGLAALAGRVTAAQSLLLGPGIARALAVRTAVLERRRPKIPNPICPNAQALAKDCVESLAQSRSQTAIELCDLLSTYEMEGLLLAHDRIASTTDRSPIASYGGSPITVPVPLGTASNNTTSNNTTTSNNSINNNTLTSAVKPATVMPSIPNNASLVNNNNNNIAKREPMSVPLGVLRDGSQDHIKIIQIEKSSEPLGATIRNEGEAVVIGRVVRGGAAEKSGLLHEGDEILEVNGIEMRGKSVNDVCALLGSMTGTLTFLVVPAGVPQILPGLGMRDPPVLHVRAHFDYDPEDDLYIPCRELGISFQKGDVLHVISRDDPNWWQAYREGEEDQTLAGLIPSQSFQHQRESMKLAIAGEVGLRTRKDINGKAGGGSTLLCARKGRKKKKKASSEHGYPLYATATADDPDPEEILTYEEVALYYPRASHKRPIVLIGPPNIGRHELRQRLMADSERFAAAIPHTSRAQREGEVPGQDYHFISRQQFESDILARKFVEHGEYEKAYYGTSLEAIRAVVASGKICVLNLHPQSLKLLRSSDLKPYTVLVAPPSLEKLRQKRIRTGEPYKEEELKEIIATARDMEARWGHLFDMIIINNDTERAYHQLLAEINSLEREPQWVPSSWLHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VDU5</accession>
    <name>A0A6E8VDU5_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="7">BTB domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VDU5"/>
    <dbReference type="EnsemblMetazoa" id="ACON000080-RC">
        <property type="protein sequence ID" value="ACON000080-PC"/>
        <property type="gene ID" value="ACON000080"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001738"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_003472"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000080"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032448"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040057"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003006">
        <property type="term" value="P:developmental process involved in reproduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048666">
        <property type="term" value="P:neuron development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18315">
        <property type="entry name" value="BTB_POZ_BAB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000138">
        <property type="entry name" value="Fruitless, isoform N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051095">
        <property type="entry name" value="Dros_DevTransReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110">
        <property type="entry name" value="BTB DOMAIN TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110:SF107">
        <property type="entry name" value="SEX DETERMINATION PROTEIN FRUITLESS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="5">
        <location>
            <begin position="30"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="6">
        <location>
            <begin position="875"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="112"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="300"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="410"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="489"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="649"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="117"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="146"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="182"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="198"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="233"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="244"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="309"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="332"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="417"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="502"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="649"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="673"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="732"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="764"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="790"/>
            <end position="825"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000080-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="912" mass="92965" checksum="12AD69CDACD24099" modified="2020-08-12" version="1">MDQQYCLRWNNHQSNLTTVLTTLLQDEKLCDVTLACEKGMVKAHQAILSACSPYFEQIFVENKHPHPIIYLRDVEVNEMRALLDFMYQGEVNVGQHNLQNFLKTAESLKVRGLTESSADRYSADTDSKLRSERIRDSRDERDSLPNASSNNSNNNNNSSSNNNNNNNTISSNNNNNSLHHGPLRDKELTEHEQLERLQQQQQQTHHQQQQHPSSHQQQSQQHPSSQHQQPTHQTHHHHHHHQHGGGVGGGGGGGGGGGGGGGSAGPVQQPSRSASIDLMQSALVDERDYLAAEDREISTVENKKKRKMSTTCDNSSPSTPSLMNERQGGYESQASSHSSFKQSPKPEDEFKVSSPAPVHPLAGHPLSGIKQELPELPVRHSLSSELMQPIPINMNADDMNNILAPGNMGSLNESGDSDAHLSHPDHPDNIDGDRMLRLAMASRHHHHRAGLHHHDLASGVVVNAVLAAGGGGGGSGCVNGSRTVGAGGMAGGGSDGPEYEGAGRGGVGSGIGGGGGGGGGRAGGGVVGATGAEKQQQNRSNHHRTTEQADREASVCAAGGVGAAAAAGVGGLGCDSGAAAAAAAAAAAAAASMLGFSGVPLPLGGSSSLVESLVEHHRLAASLGGGAVGGGSGAGGGAGSSGGSGGGLANGSPYGGGGHHLSHHHGGAAAATGHHHHQHHAAPHHHSLQQQHASSAFNSAGDARSGVAVAAALNTGGGGPPPDGSGGGSRCSKPSVTSTTPPTPASLSSSSSSSSSASSTSLCGGNGGGGGAGASGGGFLITGDPSDTIGAGGGGAGGPLRGSSGGAGGGSSGGGGNGGTSGGGSSTTRRDHNIDYSSLFIQLTGTFPTLYSCVSCHKTVSNRWHHANIHRPQSHECPVCGQKFTRRDNMKAHCKVKHPELRDRFYNHIVHM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VDW0</accession>
    <name>A0A6E8VDW0_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Microsomal glutathione S-transferase 1</fullName>
            <ecNumber evidence="5">2.5.1.18</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:16438"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="14">Homotrimer; The trimer binds only one molecule of glutathione.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the MAPEG family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8VDW0"/>
    <dbReference type="EnsemblMetazoa" id="ACON000163-RA">
        <property type="protein sequence ID" value="ACON000163-PA"/>
        <property type="gene ID" value="ACON000163"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000107"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000163"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039412"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.550:FF:000002">
        <property type="entry name" value="Microsomal glutathione S-transferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.550">
        <property type="entry name" value="Membrane associated eicosanoid/glutathione metabolism-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023352">
        <property type="entry name" value="MAPEG-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001129">
        <property type="entry name" value="Membr-assoc_MAPEG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040162">
        <property type="entry name" value="MGST1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10689">
        <property type="entry name" value="MICROSOMAL GLUTATHIONE S-TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10689:SF6">
        <property type="entry name" value="MICROSOMAL GLUTATHIONE S-TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01124">
        <property type="entry name" value="MAPEG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161084">
        <property type="entry name" value="MAPEG domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="13 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="16"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="106"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="130"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00038540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00039397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000163-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="152" mass="17348" checksum="1BB284AFD311B052" modified="2020-08-12" version="1">MATPFDSINSEAYKAYVFWSAVLVAKMLLMALLTAIQRFKNKAFASPEDTRVISKKLVPKYDDPDVERVRRAHQNDLENILPFFVIGFLYLLTNPAPWLAINLYRLVAASRILHTIVYAVVVIPQPARFLAFVGAMMPTAYMTLQTILYFML</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VDW4</accession>
    <name>A0A6E8VDW4_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Malic enzyme</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the malic enzymes family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VDW4"/>
    <dbReference type="EnsemblMetazoa" id="ACON000184-RA">
        <property type="protein sequence ID" value="ACON000184-PA"/>
        <property type="gene ID" value="ACON000184"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_008140"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000184"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039277"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004473">
        <property type="term" value="F:malate dehydrogenase (decarboxylating) (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006108">
        <property type="term" value="P:malate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05312">
        <property type="entry name" value="NAD_bind_1_malic_enz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10380:FF:000008">
        <property type="entry name" value="Malic enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000060">
        <property type="entry name" value="Malic enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10380">
        <property type="entry name" value="Malic enzyme, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015884">
        <property type="entry name" value="Malic_enzyme_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012301">
        <property type="entry name" value="Malic_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037062">
        <property type="entry name" value="Malic_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012302">
        <property type="entry name" value="Malic_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001891">
        <property type="entry name" value="Malic_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010052">
        <property type="entry name" value="PRK13529.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23406:SF80">
        <property type="entry name" value="GH17657P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23406">
        <property type="entry name" value="MALIC ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00390">
        <property type="entry name" value="malic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03949">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00072">
        <property type="entry name" value="MALOXRDTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01274">
        <property type="entry name" value="malic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00919">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00331">
        <property type="entry name" value="MALIC_ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Malic enzyme N-terminal" evidence="9">
        <location>
            <begin position="207"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="Malic enzyme NAD-binding" evidence="8">
        <location>
            <begin position="398"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="51"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="98"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000184-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="691" mass="75730" checksum="FDD11B014D79F962" modified="2020-08-12" version="1">MATTVFNGYPIMQRCQPEMDSSSSSNATKQSAAKEKPSAPSRHRVVGNELQSVKQQPSQRQQEPSRVPDRAEANQVPTHYSQPGAIGDRRSRTADRTAPTNSSSSSSSIASSNISMSTPSFNRDRLGQWAAESDGEIAGSIAGFDRLRNGKFNKGLSFSIEERQVLGIHGLMPAIVRTEEEQVQHCLELLKHYTDPLNKYIYLMGLLDRNERLFYRVLASNIGEMMPLVYTPTVGLACQKYSLVYQQPKGMYITINDKGHVYEVLKNWPESDVRAIVVTDGERILGLGDLGANGMGIPVGKLALYTALAGIKPSQCLPVTLDVGTNTQSILDDPLYVGLRHRRVTGPAYDEFVQEFMEAAVRRFGRNCLIQFEDFANSNAFRFLDQYRHDYCTFNDDIQGTASVAVAGLLASLRITRTKLADNRVLFQGAGEAALGIAQLCVMAMKRDGLTEEEARQRIWLVDSKGLIVKDRPTGGISGHKHLFAHEHAPVGTLLEAVRELKPTILIGAAAIAGAFTPEVLRTMAANNERPVIFALSNPTSKAECTAEQAYEHTDGRAVFASGSPFAPVTLGDRTFHPGQGNNSYIFPGVALGVLCAGASTIPEEIFLLSAQRLAEIVTDDDLERGSLYPPLENIKDCSIKIATHIMEYAYAEGLACTQPEPDDKEAFVRAQMYDASYKPAVPSVYAWPKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VDW9</accession>
    <name>A0A6E8VDW9_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="7">USP domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON000205-RB">
        <property type="protein sequence ID" value="ACON000205-PB"/>
        <property type="gene ID" value="ACON000205"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011676"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000205"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032329"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02257">
        <property type="entry name" value="Peptidase_C19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000041">
        <property type="entry name" value="Inactive ubiquitin carboxyl-terminal hydrolase 53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052398">
        <property type="entry name" value="Ubiquitin_hydrolase_53/54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22975:SF9">
        <property type="entry name" value="ECHINUS SPLICE FORM 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22975">
        <property type="entry name" value="UBIQUITIN SPECIFIC PROTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="6">
        <location>
            <begin position="85"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="483"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="536"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="684"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="865"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="931"/>
            <end position="1018"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1031"/>
            <end position="1070"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1129"/>
            <end position="1198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1265"/>
            <end position="1339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1351"/>
            <end position="1375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1454"/>
            <end position="1483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1526"/>
            <end position="1567"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1840"/>
            <end position="1916"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1950"/>
            <end position="1983"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1995"/>
            <end position="2017"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="2056"/>
            <end position="2098"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="408"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="1382"/>
            <end position="1416"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="1586"/>
            <end position="1613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="15"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="38"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="483"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="538"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="573"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="684"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="878"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="959"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="995"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="1061"/>
            <end position="1070"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1153"/>
            <end position="1163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1164"/>
            <end position="1179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1187"/>
            <end position="1196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1292"/>
            <end position="1307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1454"/>
            <end position="1471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="1535"/>
            <end position="1544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1853"/>
            <end position="1875"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1901"/>
            <end position="1916"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1968"/>
            <end position="1983"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="2004"/>
            <end position="2016"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="2056"/>
            <end position="2067"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="2085"/>
            <end position="2096"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000205-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="2180" mass="234849" checksum="AA46A24ED4D8CF3A" modified="2020-08-12" version="1">MISPRFNVVDVTGLGPPPPPPPPPSEKTRSSNTTGGQAPAAGMAHAPVATTAGGSSSSSYRTEADQLMHGVNFTTAHHWPVRHAKGLLNGPGQNNCFLNCAVQVLWHLDAFRRSFRQLQTHDCGGPDCIFCALKELFSQLQTSSEPALCPEPLRRALASGPLAGRRFPLGCLGDAAECFELLLHRVHQHLSPVDSDSCEAAQCVAHQRFAMRVVEQSVCECGANSEKLPFTQMVHYVSASALTSQNSLSIQHQQNITFGQLLRNAGNMGDIRDCPSACGAKIGIRRALLNRPDVVSIGVVWDSERPPADQVHAVLKAIGTTLRLCDVFQQVSDHRWAQTVHHELVGVVSYYGKHYTTFFFHTKLRVWVYFDDANVKEVGPSWEGVVEKCSRGRYQPLLLLYALPQPQQLQQQQQQQQLQQQLQQHQQELTTLAQHRRAVTPSPEKASMGSNVRRAITPTPNRALPICDYQNLTVIQSKIFASANGSSTSGPANTAAGEDGEGKEHYISRKAVQNVLSAQYQNLSVIQDKIFPSAHDLQQQQQQQQQQQHQQQQQQLRVLANGPTKQQQQLGYNGVPPNSVGMPNVSPSSSSGTASSPDGLSMPDHLNQPRRRDSGNWSGDRNSASSSSSTTLDNPYLYLVGKRQVGGSVPASPTRANNSAGAAGLGTHGAAQFYDAGYDSYSLSSTDSFPSKHQPSGGGGGAGPSAAGLLLSAAGAAVKIPDSVVLSGDCEKLCMEADQLLEKSRLLEDAHDLETALVLCHAAASKARAAMDAPYSNPHTMTFARMKHNTCVMRARSLNRRILIEKGGEMIKEQQLQQQLQQQQQQQQLHLHHQTMQQQQQQYHVLTSGGALQHRRQNSKEKLLAAGGGHHHPHHHALAGSTTAGTTAGAGTSNTASASPSKSIEIYATLPKKKVTLKLIEAEHVEPEPAVDLKAAAGGPAGGSERESRSLFARVTSSSDKDGKEGREKRSRSEDRNKALADPGLVNAKDTLRKHKEEKDANERKEKEAKSGKKQHKIRRKLLMGGLIRRKNRSMPDLTEAVGSGGPGCDPTGKGADLLEGKGGAGGAGGPAGGALHGASLDDSAVGLSVSAKDNANSGYLSEGHFDYHPISNTNPNLERSKLMRKSFHGSGRSLSVPKVPPPPPVRIGSALSAVSSGTAGASQQQQHHQQHQQQQQHQPNGAPYGSSSSASSSPSMLQMQKLPLNGVNLRHLEYVTEAAAAAHPPSGIKPFHEANVSNLSTMSSNTSMSEDSCQTIITTCAVVHQEQSPAKPQDLPPPPPTEEVDSIVRYQQQQQQQQQQQVPQQQLELPPYPSPPSTTCHSRQASEDFPPPPPAIDFEPLHEQLSEIQSLQTTGGQGKPPMMMMMMSHSSGQTGIQNTTSILAQLQAKQLQQQLQQQQQQQQQYQQHQEQLNLNNPISTSEILNETTRSEIWLKELQLKQLALKQQQQCREVSPAQQQYQPQQQQGPKLPAGPPATGGDQRSVRDLASRFEQIKLSVGQQLQSQSAAHPPNVRTGMSGLLLADQQQKANARSTPPPPPPPQPAATDVPDTVPSADFSTGSGEDVVDCPRDIGMVYRMQLPKPRYDIAQSQIQEEIREVEMLNQVVQQTLNNGAAAAAAAAASKSDVLPAAGRTKKKSVSFCDQVILVATADEDEDDGFIPNPILERVLRTAGGAGGGPTAAAAAAAVGCTDADVTPGVLLPAGNGGESVAPAAGLPERLPTLSTSITYHPIKAPSPTQQLKQQPTPPGAPVYSLAADMHKQNLEIQRQLQQQQQQYYGAVNNSSPELESYKQQQQQQYSSIPANATIFKTHQFSAAMPTTKATPSLYGVPPNMSLQLQQQQQQQQRLYGSDTGSELSFDGRNSSSSSQLTSPYMTVPHLLGDGGAGSGPPPQHAVGGYQTVSASPTTITTNTSPASNTLLRQNIATLLQHQQLGGVTPAAMRPTPPNGHTLPNVYQKPPKPLNAYQQQQPTTTTTTTPQQQQHQLQQLTSAAGYNGGVTTMPSYTQQQSSSPYQRVPLPLGYDNLYHEQPSMGLMTQQQQQQLLGLGNSVAQQQQQLQTKPIQKKVSFEPGTKGGGPDGSPGTNVSPSPSANGCQPQQQQHYQLQLQDAQQQQQQNVVGLPTRVVPIATTYGNGSAIVKPSAKAVQCNLCRKKHCILPAIYCSDCETYLARFQMPVRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VE69</accession>
    <name>A0A6E8VE69_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VE69"/>
    <dbReference type="EnsemblMetazoa" id="ACON000696-RB">
        <property type="protein sequence ID" value="ACON000696-PB"/>
        <property type="gene ID" value="ACON000696"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012822"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000696"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031578"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026063299" evidence="1">
        <location>
            <begin position="26"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000696-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="183" mass="19545" checksum="EBFA2AB2AD3A5BEB" modified="2020-08-12" version="1">MKPFHSWSSAVTILTLALCAGVALAAPNVRVKRGDEVEVIADAESAVNPLASQASKQSAQAVKDAIHPTKAPVSVTTDDTPDFDRQKVSLQVPDELFGSSFTLVTNISGRIGDLIMSTARRAGEFFWVFQPLFGKHLTIEIPTTTTTTTTPRTTTTTRAARTTTPDTALVLDLLSSATDTNDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VE77</accession>
    <name>A0A6E8VE77_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">histidine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.21</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>tRNA(His) + L-histidine + ATP = L-histidyl-tRNA(His) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17313"/>
            <dbReference type="Rhea" id="RHEA-COMP:9665"/>
            <dbReference type="Rhea" id="RHEA-COMP:9689"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78527"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.21"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.21" evidence="2"/>
    <dbReference type="RefSeq" id="XP_040224907.1">
        <property type="nucleotide sequence ID" value="XM_040368973.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VE77"/>
    <dbReference type="EnsemblMetazoa" id="ACON000735-RA">
        <property type="protein sequence ID" value="ACON000735-PA"/>
        <property type="gene ID" value="ACON000735"/>
    </dbReference>
    <dbReference type="GeneID" id="120950700"/>
    <dbReference type="CTD" id="32841"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009388"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000735"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040001"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004821">
        <property type="term" value="F:histidine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006427">
        <property type="term" value="P:histidyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00773">
        <property type="entry name" value="HisRS-like_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00859">
        <property type="entry name" value="HisRS_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01200">
        <property type="entry name" value="WHEPGMRS_RNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.800:FF:000008">
        <property type="entry name" value="histidine--tRNA ligase, cytoplasmic isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000021">
        <property type="entry name" value="Probable histidine--tRNA ligase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.10">
        <property type="entry name" value="S15/NS1, RNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00127">
        <property type="entry name" value="His_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015807">
        <property type="entry name" value="His-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041715">
        <property type="entry name" value="HisRS-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004516">
        <property type="entry name" value="HisRS/HisZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033656">
        <property type="entry name" value="HisRS_anticodon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009068">
        <property type="entry name" value="uS15_NS1_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000738">
        <property type="entry name" value="WHEP-TRS_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00442">
        <property type="entry name" value="hisS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11476:SF7">
        <property type="entry name" value="HISTIDINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11476">
        <property type="entry name" value="HISTIDYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13393">
        <property type="entry name" value="tRNA-synt_His"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00458">
        <property type="entry name" value="WHEP-TRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001549">
        <property type="entry name" value="His-tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00991">
        <property type="entry name" value="WHEP-TRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47060">
        <property type="entry name" value="S15/NS1 RNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51185">
        <property type="entry name" value="WHEP_TRS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WHEP-TRS" evidence="12">
        <location>
            <begin position="3"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="11">
        <location>
            <begin position="87"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="52"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="60"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000735-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="533" mass="58845" checksum="6470F657D7DB6FA9" modified="2020-08-12" version="1">MTAKDTLEAAITAQGDVVRKLKSAATKDKAKINEEVQKLLALKEQLKQLTVDDDGSGETAAATPAPGKPAAASEPGNRNLSLKTPKGTRDYGPEAMALRQRVLDQVVRVFRRHGAETIDTPVFELKEVLTGKYGEDSKLIYDLKDQGGEILALRYDLTVPFARYVGMGNVFNIKRYHIAKVYRRDNPQITRGRYREFYQCDFDIAGTYDPMLPDAECVKVVCEILAEVGVGDFVVKLNHRKLLDGIFEACGVPADKFRTACSSVDKLDKTPWEEVRREMVEEKGLSEEAVGRIGQFVTLHGGTELIERLAADETLRGIKVAMEGLEDMRLLLQYCEIFQVADRVSFDLSLARGLDYYTGVIYEAVLLGGGGAGGGSSADEEITVGSVAGGGRYDNLVGMFNPKRKQVPCVGVSIGVERLFSILEARAGGKTRTNETEVYVASAHKGLHLKRLEILNKLWSAGLKAEHSYKLNPKLLAQLQHCEEYQIPYAVILGDGELSRGVVKLREIATRKEEEVALDTFIEELRRRLLSAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VE90</accession>
    <name>A0A6E8VE90_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_040227963.2">
        <property type="nucleotide sequence ID" value="XM_040372029.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_049463945.1">
        <property type="nucleotide sequence ID" value="XM_049607988.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VE90"/>
    <dbReference type="EnsemblMetazoa" id="ACON000795-RB">
        <property type="protein sequence ID" value="ACON000795-PB"/>
        <property type="gene ID" value="ACON000795"/>
    </dbReference>
    <dbReference type="GeneID" id="120952554"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005333"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000795"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031730"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF251">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="98"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="137"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="167"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="193"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="228"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="257"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="323"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="355"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="390"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="415"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="446"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="480"/>
            <end position="501"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="659"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="709"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="751"/>
            <end position="777"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000795-PB"/>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="823" mass="89013" checksum="C4A5F73A650E055C" modified="2020-08-12" version="1">MSFSSNDTMRPLALQRSHSLPHLINSREDSGVALSASGSCSGLNNLGYGSDGSSGRRHRHHHHHHRHHDLKIPYGARLVADLRQLITLKQHYYPEGGWGWLVTYIGIVINCIAHGLQLSAGVFLLQTAHIFPHLTVAAGWLGALSTAVALFISPITIAVCKRKSTRLTAVIGGLVTALGCLFTSFASQFHQLFFSYGAVVGVGVGMTRDCSTLMVAQYFKKRREFVEIFIVSGSGLGIAVMSAFIKSAIDAIGWRLGLQAVTGTVFITFILGTCYRSASLYHPQRRAILHLKNQKRKIKDKNKHDDRPPFFDFSTLRSKTVRILLASTGIGAFGINTPIFYLAHEVQKDGIGDKVMILQIYLGLAWTLGCTAFGLLVVRNSVECRIARQYLCQTAIFMCGVCILALTAVHGNYHGYVMFVWIYGIFCGGYHYSLKMYTYEKVRARNFARAWGFVQFAQAIPIALGVPISGYMNDSGSGTAGYYFSSGCAIVGSVLMFLIDLHRRSVSRHKHTRANGTRHLCVSETCPQRRKLSFSQEPDNEPGIITGNTAMMIGPELLIPPLSDGIVEPINGLDKPELTCISEEGIADMDLPDNLLDDLDYVGDCITSCNKVENYLMLSEFENNLSAEVPILLDKRGRRLSLSKTKALLGGHLGATGGGTLLGHHHPECPIEHGAGYGPPGTTPSPTMGPPGDTNGLALLHPHQGQQQHHNHHHHHPHHHHHHSHQPVNGDLAKNLPPLATKSDVAPQNTIGGGGAGGGGRTGGGEAGKPAGGGTGTAAGAAATGSSTLQPQQHCRLNSKWRPSQQVGFLNNRVISVIDEASV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VE91</accession>
    <name>A0A6E8VE91_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Glutamate receptor 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="25 26">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="25">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="15">Postsynaptic cell membrane</location>
            <topology evidence="15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VE91"/>
    <dbReference type="EnsemblMetazoa" id="ACON000801-RB">
        <property type="protein sequence ID" value="ACON000801-PB"/>
        <property type="gene ID" value="ACON000801"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000694"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000801"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033446"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008328">
        <property type="term" value="C:ionotropic glutamate receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004970">
        <property type="term" value="F:glutamate-gated receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06382">
        <property type="entry name" value="PBP1_iGluR_Kainate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000064">
        <property type="entry name" value="Glutamate receptor ionotropic, kainate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000106">
        <property type="entry name" value="Glutamate receptor ionotropic, kainate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000061">
        <property type="entry name" value="Glutamate receptor, ionotropic kainate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000147">
        <property type="entry name" value="Uncharacterized protein, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019594">
        <property type="entry name" value="Glu/Gly-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001508">
        <property type="entry name" value="Iono_Glu_rcpt_met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015683">
        <property type="entry name" value="Ionotropic_Glu_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001320">
        <property type="entry name" value="Iontro_rcpt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18966">
        <property type="entry name" value="IONOTROPIC GLUTAMATE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00060">
        <property type="entry name" value="Lig_chan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10613">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00177">
        <property type="entry name" value="NMDARECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00918">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00079">
        <property type="entry name" value="PBPe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="9 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 22" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0770">Synapse</keyword>
    <keyword evidence="4 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="22">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026083170" description="Glutamate receptor 1" evidence="22">
        <location>
            <begin position="29"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="552"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="628"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="815"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor C-terminal" evidence="23">
        <location>
            <begin position="421"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor L-glutamate and glycine-binding" evidence="24">
        <location>
            <begin position="431"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="871"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="891"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="507"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="512"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="678"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="679"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="729"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="site" description="Crucial to convey clamshell closure to channel opening" evidence="18">
        <location>
            <position position="657"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with the cone snail toxin Con-ikot-ikot" evidence="18">
        <location>
            <position position="684"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with the cone snail toxin Con-ikot-ikot" evidence="18">
        <location>
            <position position="775"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="741"/>
            <end position="799"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00072754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="SMART" id="SM00079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="SMART" id="SM00918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000801-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="924" mass="103438" checksum="77BA52BA20E13070" modified="2020-08-12" version="1">MTGRWAHPCWWAACLLALLLHGPAPARALPDAIRIGGLFHPDDDHQEIAFRYAVEKINSDRTILPRSKLLAQIERISPQDSFLASKRVCHLLGVGVAAIFGPQSSHTASHVQSICDTMEIPHLETRWDYRLRRESCLVNLYPHPSTLSKAYVDLVAAWGWKSFTIIYETNEGLVRMQELLKAHGLSDYPITVRQLSDSGDYRPLLKQIKNSAESHIVLDCSTERIYEVLKQAQQIGMMSDYHSYLITSLDLHTINLDEFKYGGTNITAFRLVDPENPEVAQAIHNWTIGEARLGKKVDFKTAVSAETALMYDAVHLFAKALHDLDTSQQIDIHPLSCDTQDTWPHGYSLINYMKIVEMRGLTDVIKFDHQGFRSDFVLDIVELGPQGLRKSGTWNSTSGVNFTRTYGEQQKEIVEILQNKTLIVTTILSAPYCMRKDSAEKLTGNSQFEGYAIDLIHEISKILGFNYTIRLAPDGRYGSHNKETGEWDGMIKELLEQRADLAIADLTITFDREQVVDFTMPFMNLGISVLYRKPVKQPPNLFSFLSPLSLDVWIYMATAYLGVSVLLFILARFTPYEWENPHPCNSEPLFLENSFTLLNSLWFTIGSLMQQGCDIAPKAVSTRMVAGMWWFFTLIMISSYTANLAAFLTVERMDSPIESAEDLAKQTKIKYGALRGGSTAAFFRDSNFSTYQRMWSFMESARPSVFTASNIEGVERVVKGKGSYAFLMESTSIEYVIERNCELTQVGGMLDSKGYGIAMPPNSPFRTAISGAVLKLQEEGKLHILKTRWWKEKRGGGSCRDDTSKSSSTANELGLANVGGVFVVLMGGMGVACVIAVCEFVWKSRKVAVEERVSLCSEMASELRFALKRNRAQPKHAGSGGTLRTASSRASPKDSASEPSSEHGRFHPLGPPYAQYEFDPKAVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VFY9</accession>
    <name>A0A6E8VFY9_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Rab-GAP TBC domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_040218474.2">
        <property type="nucleotide sequence ID" value="XM_040362540.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VFY9"/>
    <dbReference type="EnsemblMetazoa" id="ACON003341-RB">
        <property type="protein sequence ID" value="ACON003341-PB"/>
        <property type="gene ID" value="ACON003341"/>
    </dbReference>
    <dbReference type="GeneID" id="120947319"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005768"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003341"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042393"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000010">
        <property type="entry name" value="Putative USP6 N-terminal-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000019">
        <property type="entry name" value="USP6 N-terminal like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.750">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF25">
        <property type="entry name" value="RAB-GAP TBC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="3">
        <location>
            <begin position="144"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="405"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="448"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="8"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="474"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003341-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="550" mass="64287" checksum="72349275564221EE" modified="2020-08-12" version="1">MTRYDRYSLSTDQQQQKQTLNPPSAAAAADRKLDTVAVAPGTTMTDEELLLLERANAERDEIFLRYDQGRKCDSIDAWEDPMFEVYTQADRYGFLHPVAPAQSKEAELLQHRIEMERVKKWVKMHKNWDAAATKENLRRRVMKGIPDRMRSAIWRKLLDLDRQIRENGGMYDRMLDCARRHSPDIRQIDFDVNRQFRNHVFYRERYSVKQQSLFRVLAAYSMYNTEVGYCQGMSTVAAVLLMYFDEEDTFWALDVLMTNQRYAMHGLYIVGFPKLMRFLAHHDRILTKCLPKVKKHLDKHEVHSVLYSLKWFFVIFIERIPFSLCLRVWDIYMMYGERVLTAMAYTILKVHKTKLLRMKDMDQVTDFLQTSLHKQFGYDDDYVIKALQSSMDELKKLKLLNLPPPSANELPTMPRGQTVEPSTKARIGHRNEQFSEREIALKETVLYRSESKNEEEEEQYNNGVQGAGDRAATAEDEDDERTEKDYDDITEDTVSQLHTGVSTSSLRTIQSFTTFDTGTSLATSLNSLVIIDSFDDYCDNDRIIEESTRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VFZ3</accession>
    <name>A0A6E8VFZ3_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Phosphoenolpyruvate carboxykinase [GTP]</fullName>
            <ecNumber evidence="4">4.1.1.32</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>oxaloacetate + GTP = phosphoenolpyruvate + GDP + CO2</text>
            <dbReference type="Rhea" id="RHEA:10388"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="4.1.1.32"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.32" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8VFZ3"/>
    <dbReference type="EnsemblMetazoa" id="ACON003350-RB">
        <property type="protein sequence ID" value="ACON003350-PB"/>
        <property type="gene ID" value="ACON003350"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005144"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003350"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042887"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004613">
        <property type="term" value="F:phosphoenolpyruvate carboxykinase (GTP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071333">
        <property type="term" value="P:cellular response to glucose stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046327">
        <property type="term" value="P:glycerol biosynthetic process from pyruvate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006107">
        <property type="term" value="P:oxaloacetate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019543">
        <property type="term" value="P:propionate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033993">
        <property type="term" value="P:response to lipid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042594">
        <property type="term" value="P:response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00819">
        <property type="entry name" value="PEPCK_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.228.20:FF:000005">
        <property type="entry name" value="Phosphoenolpyruvate carboxykinase [GTP], mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.449.10:FF:000003">
        <property type="entry name" value="Phosphoenolpyruvate carboxykinase, cytosolic [GTP]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.228.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.449.10">
        <property type="entry name" value="Phosphoenolpyruvate Carboxykinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.8.10">
        <property type="entry name" value="Phosphoenolpyruvate Carboxykinase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00452">
        <property type="entry name" value="PEPCK_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018091">
        <property type="entry name" value="PEP_carboxykin_GTP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013035">
        <property type="entry name" value="PEP_carboxykinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008209">
        <property type="entry name" value="PEP_carboxykinase_GTP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035077">
        <property type="entry name" value="PEP_carboxykinase_GTP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035078">
        <property type="entry name" value="PEP_carboxykinase_GTP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008210">
        <property type="entry name" value="PEP_carboxykinase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003253">
        <property type="entry name" value="PRK04210.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11561">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE CARBOXYKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11561:SF0">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE CARBOXYKINASE [GTP]-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00821">
        <property type="entry name" value="PEPCK_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17297">
        <property type="entry name" value="PEPCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001348">
        <property type="entry name" value="PEP_carboxykinase_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68923">
        <property type="entry name" value="PEP carboxykinase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53795">
        <property type="entry name" value="PEP carboxykinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00505">
        <property type="entry name" value="PEPCK_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="10" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphoenolpyruvate carboxykinase GTP-utilising N-terminal" evidence="15">
        <location>
            <begin position="51"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphoenolpyruvate carboxykinase C-terminal P-loop" evidence="14">
        <location>
            <begin position="282"/>
            <end position="645"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00051400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00058806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00072283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF17297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003350-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="649" mass="71573" checksum="F8D97B124A1B1AF1" modified="2020-08-12" version="1">MVKLFALLRLPTTLGCSPKLKTLPKNLYNHLRGFPVVNGEVSLLSDKVRRFVEESAAMCQPDRIHIVDGSESESSTLLKTLHSQGTIKPLPKYDNCWLARTNPADVARVESKTFICTERPEQAIPTPKPGVNGALGNWISPADYDRAIMSRFPGCMKGRTMFVVPFSMGPIASPLSKIGIEITDSAYVVASMRIMTRMGAEVLDKLSDNSDFIRCLHSVGTPANGKIAVPSWPCDPERTIILHKPANNEIVSYGSGYGGNSLLGKKCFALRIGSTIAQREGWLAEHMLILGITDPNGVKKYIAAAFPSACGKTNLAMMNPTLPGYKIECVGDDIAWMKFDSKGQLRAINPENGFFGVAPGTSSETNPNAMETIYKNTIFTNVASTSDGGVFWEGMEKEVSSDVEITDWLGNPWKLGESKTPAAHPNSRFCAPAGQCPIIDPAWEDSEGVPISAILFGGRRPQGVPLVYEANSWSHGVFVGSSMRSESTAAAEHKAKVIMNDPFAMRPFFGYNFGDYLKHWLSMEQRASAAGGHAPKIFHVNWFRKDANGKFLWPGFGENSRVLEWILHRIDDADCYRDTPIGRVPTEGSLNLDGLSSPVNERELFSIPKDFWLREVEEVKQYYDNQLPQDLPAEIASELEQLKSRIEKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VFZ8</accession>
    <name>A0A6E8VFZ8_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VFZ8"/>
    <dbReference type="EnsemblMetazoa" id="ACON003304-RB">
        <property type="protein sequence ID" value="ACON003304-PB"/>
        <property type="gene ID" value="ACON003304"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012735"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003304"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042772"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003304-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="87" mass="9676" checksum="AC897B73B9728E58" modified="2020-08-12" version="1">MTIPSVTYKQFGETVVPVAPVGTKRWIVLYIMVTAILLTTLACWNNLSGGSLIEMFEDDSDAPPPHTEYAKMARYLVHKAEWVSMGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VFZ9</accession>
    <name>A0A6E8VFZ9_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="9">PHB domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the band 7/mec-2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040221262.1">
        <property type="nucleotide sequence ID" value="XM_040365328.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VFZ9"/>
    <dbReference type="EnsemblMetazoa" id="ACON003352-RA">
        <property type="protein sequence ID" value="ACON003352-PA"/>
        <property type="gene ID" value="ACON003352"/>
    </dbReference>
    <dbReference type="GeneID" id="120948701"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000005"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003352"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030318"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13435">
        <property type="entry name" value="SPFH_SLP-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.479.30:FF:000002">
        <property type="entry name" value="band 7 protein AGAP004871"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2090">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="entry name" value="Band 7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043202">
        <property type="entry name" value="Band-7_stomatin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001972">
        <property type="entry name" value="Stomatin_HflK_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10264:SF19">
        <property type="entry name" value="AT06885P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10264">
        <property type="entry name" value="BAND 7 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00721">
        <property type="entry name" value="STOMATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="Band 7/SPFH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="38"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="Band 7" evidence="8">
        <location>
            <begin position="58"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="299"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="311"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003352-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="331" mass="35816" checksum="E610814AF677C47C" modified="2020-08-12" version="1">MTAALMEPTPSTSSAAVIINDDKRRTSAEADSIGCVEVLATVCSIVLMVLTLPISLFLCFKVVQEYERAVIFRLGRLRSGGARGPGVFFVLPCIDNYCKVDLRTVSFDVPPQEVLTRDSVTVSVDAVVYYRIRDPLNAVVQVANYSHSTRLLAATTLRNVLGTRNLSELLTEREAISHSMQVTLDEATDPWGVQVERVEIKDVSLPDSLQRSMAAEAEAAREARAKVIAAEGEMKSSRALKEASDIMCESPAALQLRYLQTLSSIAGEKNSTIVFPLPIELIGPLMNFTSSLGVQRTTAPVRPMAPPAAPPSTTTSTSSPLGDPSQQQSFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VIA0</accession>
    <name>A0A6E8VIA0_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="6">PDZ domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VIA0"/>
    <dbReference type="EnsemblMetazoa" id="ACON003939-RC">
        <property type="protein sequence ID" value="ACON003939-PC"/>
        <property type="gene ID" value="ACON003939"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010454"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003939"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032779"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031941">
        <property type="term" value="C:filamentous actin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001725">
        <property type="term" value="C:stress fiber"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051371">
        <property type="term" value="F:muscle alpha-actinin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061061">
        <property type="term" value="P:muscle structure development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23068">
        <property type="entry name" value="PDZ_ZASP52-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000055">
        <property type="entry name" value="PDZ and LIM domain protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050604">
        <property type="entry name" value="PDZ-LIM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24214">
        <property type="entry name" value="PDZ AND LIM DOMAIN PROTEIN ZASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24214:SF38">
        <property type="entry name" value="PDZ AND LIM DOMAIN PROTEIN ZASP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0440">LIM domain</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PDZ" evidence="5">
        <location>
            <begin position="4"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="113"/>
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        </location>
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            <begin position="138"/>
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        </location>
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            <begin position="225"/>
            <end position="236"/>
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    </feature>
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            <end position="263"/>
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        <location>
            <begin position="411"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
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        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003939-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="610" mass="68334" checksum="21B074B60CAE5747" modified="2020-08-12" version="1">MVLDLNFSRYDNTTPFGFKLVGGADFDVPLTVVKILPGSIAEDVGMHMGDVVVRINDIPTGNMSYYDAHQLLACAGNQFVLGILRAREVTPSQRLIKEKTPSGEVEYINTSPKPFWTPNVGSPSPLAVHHPAKEEPRQQQQQPDEQIPEVPITDEQIAEILSGEAEVLKQHNVIGVNFNKMIPKAGVFKQSEVFKTLNSELVQSEEEDKKWTTFLQKPSRPPPKTPEERKLERNPPTERYQVRIVKQPKPKIAPDYKPPKSPEPEPEPEPIPMYDEYLNERQEVEVETIKTLVEEPVVPSEPMAGEGEAQQPGAPNPTEEEPSEEFREQLQNVQKQLEALSALPNTIQATIAALTEQLAVLAAPKRKSKSISPRPEEADPSAGGEGTEQSAPEAIVPEVSETFEVTEFSRADSSGSGDQSSSVDEGEHIHYTEEELEQLEKRMKEHDIEWTNQNDKDSDSSSQHNAGGDIRTAVQDELKLQVVKKKKKAVSAFGPLTPQERPIYLPGGRKWRTAKDAFNEQFIAEVISSQAELIQGTTLGVNFLKYQKPEKDLSFIKNSDVYKLVHDMDPPKSGIELRPEMVVAEEDVRKVMKDETIFQPRKSKVFLYQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VIA2</accession>
    <name>A0A6E8VIA2_ANOCL</name>
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        <submittedName>
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        </submittedName>
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        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
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    <dbReference type="EnsemblMetazoa" id="ACON004169-RB">
        <property type="protein sequence ID" value="ACON004169-PB"/>
        <property type="gene ID" value="ACON004169"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009035"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004169"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033048"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050938">
        <property type="entry name" value="Collagen_Structural_Proteins"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37456:SF4">
        <property type="entry name" value="COLLAGEN ALPHA-1(XXIII) CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37456">
        <property type="entry name" value="SI:CH211-266K2.1"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="223"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="340"/>
            <end position="698"/>
        </location>
    </feature>
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            <begin position="13"/>
            <end position="24"/>
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    </feature>
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            <end position="239"/>
        </location>
    </feature>
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        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004169-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VIC3</accession>
    <name>A0A6E8VIC3_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glutathione transferase</fullName>
            <ecNumber evidence="3">2.5.1.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">GST class-theta</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GST superfamily. Theta family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="3"/>
    <dbReference type="RefSeq" id="XP_040221732.1">
        <property type="nucleotide sequence ID" value="XM_040365798.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VIC3"/>
    <dbReference type="SMR" id="A0A6E8VIC3"/>
    <dbReference type="EnsemblMetazoa" id="ACON004164-RA">
        <property type="protein sequence ID" value="ACON004164-PA"/>
        <property type="gene ID" value="ACON004164"/>
    </dbReference>
    <dbReference type="GeneID" id="120948928"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001564"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004164"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039141"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03177">
        <property type="entry name" value="GST_C_Delta_Epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03045">
        <property type="entry name" value="GST_N_Delta_Epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000034">
        <property type="entry name" value="glutathione S-transferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000007">
        <property type="entry name" value="Glutathione S-transferase 1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43969">
        <property type="entry name" value="GLUTATHIONE S TRANSFERASE D10, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43969:SF9">
        <property type="entry name" value="GLUTATHIONE S TRANSFERASE D10, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01153">
        <property type="entry name" value="Main.4:_Theta-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="7">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="8">
        <location>
            <begin position="86"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00041523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004164-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="209" mass="23445" checksum="3B262EEE21FAA9D0" modified="2020-08-12" version="1">MDFYYLPGSAPCRAVQMTAAAVGVELNLKLTDLMKGEHMKPEFLKLNPQHCIPTLVDNGFALWESRAIQIYLAEKYGKDDKLYPKDPQKRAVVNQRLYFDMGTLYQRFADYHYPQIFAKQPANPENEKKMKDAVGFLNTFLEGQEYAAGNDLTIADLSLAATIATYEVAGFDFAPYPNVAAWFARCKANAPGYALNQAGADEFKAKFLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VKA9</accession>
    <name>A0A6E8VKA9_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">CARMIL C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CARMIL family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VKA9"/>
    <dbReference type="EnsemblMetazoa" id="ACON004841-RA">
        <property type="protein sequence ID" value="ACON004841-PA"/>
        <property type="gene ID" value="ACON004841"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010900"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004841"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042041"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034315">
        <property type="term" value="P:regulation of Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000009">
        <property type="entry name" value="F-actin-uncapping protein LRRC16A isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031943">
        <property type="entry name" value="CARMIL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041245">
        <property type="entry name" value="CARMIL_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051279">
        <property type="entry name" value="PP1-Reg/Actin-Interact_Protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24112:SF66">
        <property type="entry name" value="LEUCINE-RICH REPEAT, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24112">
        <property type="entry name" value="LEUCINE-RICH REPEAT, ISOFORM F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17888">
        <property type="entry name" value="Carm_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16000">
        <property type="entry name" value="CARMIL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CARMIL pleckstrin homology" evidence="11">
        <location>
            <begin position="25"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="CARMIL C-terminal" evidence="10">
        <location>
            <begin position="775"/>
            <end position="1073"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="944"/>
            <end position="964"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1047"/>
            <end position="1068"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1093"/>
            <end position="1112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1118"/>
            <end position="1205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="944"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1144"/>
            <end position="1154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004841-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1271" mass="139230" checksum="71ACB88A2D13739F" modified="2020-08-12" version="1">MSTRSQLTKDLNESVKTLLGRHTKILVKYMVKLEVKSDKTENRVLVFTPVRVYLLTAKVPTRIDCHFHYLDIQSIESRKPTHFTITTNDKSYSFSTIGDAGSFTSNADVILTDLSSAIKQIFPTVPLRYIIRKIDVNPIERTSIFSDELRPSDPRNVGPCGGFSMQYACMCDLHAVQYREEVAWDIDTIYLSHDARVLNLRDFDHLEQKDLMAIVAALEYNTFFRGLKASHTRLSTETLERVLQVLRRSLWLEELHLESLGLKSDFIHKLAVAVISNSAPALRSIDLSHNVIEDKGATHLAGPIAKMSKGFSKLALAHCGLTAKGVNQLAHSLSLNQNISNSLTYLDLSGNILKDDGNNLYNFLAQPNVIEHLDISRTDTTLESVFGALLRGCATHLLHLNVSHNNFGTKKGKEIPPSFKQFFTSSLSLKHLNIASCKLPLEALKHLLLGLACNESTAGLYLDLSGNSLGSQGAHVLESCIHGVRVLACLDISDNNMDYELANVLTAIGKNPSIRSLYMSKCFTGMKLKHIGTVMDSLVNLIQKEDFQLQELVLAENRLKTDLHNFINALGSNQHLQMLDITGNLMGDIGARLLAKALQINNKLKTIALDRNNFTLQGYADIVYALENNFSVRNMPFPLHDIAPCLKQHPERTDVLMKKMQELLFRNGNGFKRTHGQGFRLQHGFLLSSTHQVLDKLVAETQETMSSRQSSLDGSLSRLLEDAENCKQLLPKLQESVRCDPHPIEVRLGKMKTELHHSIKSYLEETMETMLRTGIEQCPKTLGSQVVLTELRKGCEERLTISEEFLQSCLVNSAGGEVMNRISEVEQSLASLISDRATDEVLEALTRYRKGLGIAEGPLSYTDDLHSLDGSKHNSSQSTEPIANFELRGLDLPKLGLDSPTATPQLQHKRRSIARKVRPQSVVGLGIENLSLANIPDVTETSMASVSRSSSNNSGSKKYESSNAKEDECCDSITELPSQSLTLQHLVKGRPKRAKTRAPKKPVITDTVTAGGTVVNEGIESFFRPGSATPSTLTPLVSPTSEECSSLSFVDSPTLSRDDGGRFSNVTSGETTPILEERKPLKLDRSSPLLKGVNWTSRSRSSDNLEKVSPSVNRKSPLVKAATEVVETPVRESRPVDNGAATPITGSKSPSNESVRNHHVESAHGASRLGNGTGQKTPIITPKPRPWSVLGHESKNEFNEPSVDSIDTDGDLVLISGQTPGGSIVGITPGAISSAGVTGGSILGITPGAVIEKKSVRDMAAGLNKLGGDAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VKB2</accession>
    <name>A0A6E8VKB2_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Microtubule-associated protein RP/EB family member 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MAPRE family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040229122.1">
        <property type="nucleotide sequence ID" value="XM_040373188.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VKB2"/>
    <dbReference type="EnsemblMetazoa" id="ACON004886-RB">
        <property type="protein sequence ID" value="ACON004886-PB"/>
        <property type="gene ID" value="ACON004886"/>
    </dbReference>
    <dbReference type="GeneID" id="120953348"/>
    <dbReference type="CTD" id="35584"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_013056"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004886"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038747"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1430:FF:000005">
        <property type="entry name" value="Eb1, isoform E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000007">
        <property type="entry name" value="Microtubule-associated protein, RP/EB family, member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004953">
        <property type="entry name" value="EB1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036133">
        <property type="entry name" value="EB1_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027328">
        <property type="entry name" value="MAPRE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10623">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEIN RP/EB FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03271">
        <property type="entry name" value="EB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140612">
        <property type="entry name" value="EB1 dimerisation domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51230">
        <property type="entry name" value="EB1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5 9" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="11">
        <location>
            <begin position="14"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="EB1 C-terminal" evidence="12">
        <location>
            <begin position="200"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="176"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="191"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004886-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="281" mass="31967" checksum="604D53F87F7F879C" modified="2020-08-12" version="1">MAVNVYSTNVTTDNLSRHDMLSWVNDCLRSQFTKIEELCTGAAYCQYMDMLFPGSVPMKRVKFRTNLEHEYIQNFKILQAAFKKMNVDKIIPIDKLIKGRFQDNFEFLQWFKKFFDANYDGREYDALLARDNLPMGMGSGAGAPKGNGVSAVAPPRKMAIGTTAPRDRIKPIAPTRQMVQSPPSRIGGGVNVNTSKSSSQNSVTNQQIEELNSQISDMRLNFEGLEKERDFYFSKLRDIEILCQDDEQSQSPFVQKILEILYATEDGFAPPDEIPPEEEEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VKB6</accession>
    <name>A0A6E8VKB6_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Ribose-phosphate pyrophosphokinase 2</fullName>
            <ecNumber evidence="5">2.7.6.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Phosphoribosyl pyrophosphate synthase II</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the synthesis of phosphoribosylpyrophosphate (PRPP) that is essential for nucleotide synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>D-ribose 5-phosphate + ATP = 5-phospho-alpha-D-ribose 1-diphosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15609"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.6.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Homodimer. The active form is probably a hexamer composed of 3 homodimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ribose-phosphate pyrophosphokinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.1" evidence="5"/>
    <dbReference type="RefSeq" id="XP_040240765.1">
        <property type="nucleotide sequence ID" value="XM_040384831.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VKB6"/>
    <dbReference type="EnsemblMetazoa" id="ACON004890-RB">
        <property type="protein sequence ID" value="ACON004890-PB"/>
        <property type="gene ID" value="ACON004890"/>
    </dbReference>
    <dbReference type="GeneID" id="120961151"/>
    <dbReference type="CTD" id="39132"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006499"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004890"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_036890"/>
    <dbReference type="UniPathway" id="UPA00087">
        <property type="reaction ID" value="UER00172"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002189">
        <property type="term" value="C:ribose phosphate diphosphokinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004749">
        <property type="term" value="F:ribose phosphate diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006015">
        <property type="term" value="P:5-phosphoribose 1-diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009156">
        <property type="term" value="P:ribonucleoside monophosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000011">
        <property type="entry name" value="Putative ribose-phosphate pyrophosphokinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00583_B">
        <property type="entry name" value="RibP_PPkinase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000842">
        <property type="entry name" value="PRib_PP_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029099">
        <property type="entry name" value="Pribosyltran_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005946">
        <property type="entry name" value="Rib-P_diPkinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037515">
        <property type="entry name" value="Rib-P_diPkinase_bac"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002320">
        <property type="entry name" value="PRK01259.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01251">
        <property type="entry name" value="ribP_PPkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210">
        <property type="entry name" value="RIBOSE-PHOSPHATE DIPHOSPHOKINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210:SF32">
        <property type="entry name" value="RIBOSE-PHOSPHATE PYROPHOSPHOKINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14572">
        <property type="entry name" value="Pribosyl_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13793">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01400">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00114">
        <property type="entry name" value="PRPP_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ribose-phosphate pyrophosphokinase N-terminal" evidence="18">
        <location>
            <begin position="106"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="41"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="58"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="68"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="86"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00026067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00040333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00041814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF13793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004890-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="420" mass="45112" checksum="706A24343135F63D" modified="2020-08-12" version="1">MGRKGSKQTKLPPDSQEVRSSHPIRARTLIRSNLEKASVISNTSNSSSGGTPACGSNLVHSLSSATPSTGGSSISDSGSDSKHPTDSISTPSVDPHQLLQSRMPNIKVFSGSSHPDLASRIVDRLGIDLGKVVTKKFSNLETCVEIGESVRGEDVYIVQSGSGEINDNLMELLIMINACKIASASRVTAVIPCFPYARQDKKDKSRAPISAKLVANMLSVAGADHIITMDLHASQIQGFFDIPVDNLYAEPAVLKWIRENIAEWRNSIIVSPDAGGAKRVTSIADRLNVEFALIHKERKKANEVASMVLVGDVKDRVAILVDDMADTCGTICHAADKLVEAGATKVYAILTHGIFSGPAISRINNACFEAVVVTNTIPQDGHMKDCPKIQCIDVSMMFAEAVRRTHNGESVSYLFSNVPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VKC3</accession>
    <name>A0A6E8VKC3_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Metalloendopeptidase</fullName>
            <ecNumber evidence="11">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">Ngousso</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="10 11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="10 11">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="11"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8VKC3"/>
    <dbReference type="EnsemblMetazoa" id="ACON004620-RC">
        <property type="protein sequence ID" value="ACON004620-PC"/>
        <property type="gene ID" value="ACON004620"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_008540"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004620"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031589"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04280">
        <property type="entry name" value="ZnMc_astacin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000015">
        <property type="entry name" value="Meprin A subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034035">
        <property type="entry name" value="Astacin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001506">
        <property type="entry name" value="Peptidase_M12A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10127">
        <property type="entry name" value="DISCOIDIN, CUB, EGF, LAMININ , AND ZINC METALLOPROTEASE DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10127:SF780">
        <property type="entry name" value="METALLOENDOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01400">
        <property type="entry name" value="Astacin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00480">
        <property type="entry name" value="ASTACIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51864">
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    <keyword evidence="3 11" id="KW-0732">Signal</keyword>
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    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
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        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
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        <location>
            <begin position="23"/>
            <end position="290"/>
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            <begin position="94"/>
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        </location>
    </feature>
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        <location>
            <position position="187"/>
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        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
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        <location>
            <position position="191"/>
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
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        <location>
            <position position="197"/>
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
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            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023180"/>
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            <dbReference type="RuleBase" id="RU361183"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51864"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004620-PC"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="290" mass="31804" checksum="34B24EBA155BE844" modified="2020-08-12" version="1">MSVSQILRVLAIGCFVIGCTQANVIMYDTEENSIDGSSDFIDLTHLGPNLFGEPDEEVGKLVANFNPETDAGNVEELGSYVEGDMLIDRPEGRNGLSNTATRWPNGVVPFVISGNFDAKGMQLIEQAINEYHTKTCIRFVPRMGEKNYVSFESSSSGCWSSVGMIGGKQAVNLQIPGCTTLVGTAIHEMMHALGFLHEQNREDRDDWVRIRYENIKAGTSNNFEKAKKGTTNSFGVAYDYASIMHYSSNAFSTNGNPTIEAKKPFSGDMGQRIGFSKSDLAKINLMYGCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VKC9</accession>
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        <submittedName>
            <fullName evidence="6">SH3 domain-containing protein</fullName>
        </submittedName>
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        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
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            <taxon>Nematocera</taxon>
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            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
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                <consortium name="EnsemblMetazoa"/>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="6">Ngousso</strain>
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        <property type="gene ID" value="ACON004625"/>
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            <end position="484"/>
        </location>
    </feature>
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            <end position="91"/>
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            <begin position="1"/>
            <end position="11"/>
        </location>
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        </location>
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        </location>
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        </location>
    </feature>
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    </evidence>
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    </evidence>
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    </evidence>
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            <dbReference type="PROSITE" id="PS50002"/>
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        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004625-PA"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="484" mass="54080" checksum="3C1A57BBFAD4E968" modified="2023-06-28" version="2">MWKSTAGRDIDTTGVNQGEDDDWETDPDFVNDVSEQEQRWGSKTVEGSGRNAAAIDMQQLREETERADSEKKRKEGPKASHGYGGKFGVEKDRMDKSAVGHEHIEKVEKHASQKDYVSGFGGKFGVQKDRVDKSAHGWDHVEKVDKHESQKDYKTGFGGKFGVQQDRQDKSAVGWDHIEAPQKHESQIDHKVGFGGKFGVQNDRMDKSAVGFQEQDKIGTNYTKVKPDIGSAKPSNLRAKFENFAATAEEEARKRADEQKRLREEKDRCDREEAAKRLNHSAESAEPKKPERKGPINTGREAGVSSAISNFNNPQENITKEKTRKDPIVLPKQDEPPKFVQPDVIPSSDSTSVPTAKTKEDQPEESAGHAVQEPLPVARTSYSATPIAVEETDEAPVTDSTAHQEQTTDTVPVQDDVEEFILSPDNPGIQAIALYDYQAAADDEISFDPDDKITHIEMIDEGWWRGWCNNKYGLFPANYVQLLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VKE3</accession>
    <name>A0A6E8VKE3_ANOCL</name>
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        <submittedName>
            <fullName evidence="5">FH2 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="EnsemblMetazoa" id="ACON004873-RA">
        <property type="protein sequence ID" value="ACON004873-PA"/>
        <property type="gene ID" value="ACON004873"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_003524"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004873"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031522"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Formin, FH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015425">
        <property type="entry name" value="FH2_Formin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042201">
        <property type="entry name" value="FH2_Formin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056771">
        <property type="entry name" value="FH3_FHOD1-3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041387">
        <property type="entry name" value="FHOD1_GBD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014768">
        <property type="entry name" value="GBD/FH3_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45920">
        <property type="entry name" value="FORMIN HOMOLOGY 2 DOMAIN CONTAINING, ISOFORM I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45920:SF4">
        <property type="entry name" value="FORMIN HOMOLOGY 2 DOMAIN CONTAINING, ISOFORM I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02181">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24959">
        <property type="entry name" value="FH3_FHOD1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18382">
        <property type="entry name" value="Formin_GBD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00498">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51444">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51232">
        <property type="entry name" value="GBD_FH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GBD/FH3" evidence="3">
        <location>
            <begin position="55"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="domain" description="FH2" evidence="4">
        <location>
            <begin position="928"/>
            <end position="1319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="349"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="674"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="801"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1371"/>
            <end position="1460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="373"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="400"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="689"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="801"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="821"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1372"/>
            <end position="1392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004873-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1460" mass="162510" checksum="EA6A70E83DCA6F98" modified="2020-08-12" version="1">MEPDELITIRVQYLVDSDPFNSLSMYPIPSRAPVFSFASAVPLATQLGALLRHLGAPQRVSNLDDAALQVYKDGDYGAYLDLESSLAEQSEDIEGLNANRKNSLVVRTQLSVRVHSIIEKLLSSEGRELRRVLFSLKQIFQEDKDLVHGFVAHGGLNCLVQIGSEADQNYQNYILRALGQVMLYVDGMNGVMKHSPTIQWLYSLIASRYRLVVKTALKLLLVFVEYCEGNCYLLVSAIRFMDTARGVVPWNNIMRLLKDYENADTELLIYATSLINKTLSGLSDQDSFYDESDFLEQQGMEGIIQRYMSRPGTDLDLLDQLQLYESVLRFEDGECDGIRIPDNTVRKTLRYRSTGNDTTDRRKSRRHSTGTAPPQPPSIAPPPPPPPPPMPAYTMYSAPPSGPPPPPPPPLPPPVVPVQLYGGLQLPGQRQQDEDSSSSAHSGDHLPGTGYHDPSRDTTGVTPGLRRRRERAERQMNLMREQQELGQFPNGIVPTGEDGNNATNVGYHAGHRNGASNYRRQSGGTGPLTSSSLLAPPTIHEEDKLQLRLNGGLTSSVILHKQQPSNHIEHTNGTGHANGHGAANATSQLDSVEYRNSRNLLLKKHGTSGGVSMVPVSISNGTAPDRYKADLNGNSVRSTRTCTGVLSEQDDENKSPNIKTQMMGLSVAYGKQLANGKDSSDTSYHRRSATSTAYERSNGSLNGGHVEPKGQDLPPVPGKPLSLALLKVCQIFQIRSSLYLSRFLPFLAESDNEDKKLILQLKKDHTVKDLTQKLSCLPLSPQEEKPQNRIGDMSGLISKAKEGLAKSKSNKDISRSPSVDNDIKKLANGEPKKSENELHWEEIVKNMTRKLSLCDLDFTDLTSDDDKDLLAPRGFGGAVPPPPPPSGMLMNGMPPPPSMMRPPPTNLVPLSGSMMNGNGMPQPNSLDGSAAIKKNKKTVKLFWKEVRDDMLPAVIQRTIWDELPEAIVDTQKLEHLFESRAKDLMTKKQQELNKNKEIIVLDHKRSNAINIAMTKLPPPRAIKAAILKMDSTVVTREGIDKLLNMLPTDEERCKIQEAQMLNPELPLGTAEQFLLTLSSISELGARLKLWAFKLDFENIEKEIAEPLNDLKQGIELLKSNLTFKCILSTLLNIGIFLNGAPVKGFQIDYLAKVPEVKDTVHKHSLLHHLCHMVMESQANTTDLYSEIGPITRASKADFNELAHNIVYLETECKASWDRLKLIGKHDTAPHLKQKLIDFLADCAERIIILDIVHRRVLNRYRKFLLWLGVPQLRIAESRPNEFCRIVSEFALEYRTTRERVQQQIEKKANHRERNKTRGKLIVDVAKLKTQEDRADDELRTLLGTPTKGPGGDEVEMSGTLTWRRRRGDATASPVTLMNSHPTVPSPGHNSVSVMGGRDESFTDGDDEILESLVKTATKTATPRPMQRERKRTRQADRKSFKRSRTREGFTMERTGSSPPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VKE8</accession>
    <name>A0A6E8VKE8_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Paramyosin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Paramyosin is a major structural component of many thick filaments isolated from invertebrate muscles.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Myofibril</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the paramyosin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VKE8"/>
    <dbReference type="EnsemblMetazoa" id="ACON004877-RA">
        <property type="protein sequence ID" value="ACON004877-PA"/>
        <property type="gene ID" value="ACON004877"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009545"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004877"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035792"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030016">
        <property type="term" value="C:myofibril"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032982">
        <property type="term" value="C:myosin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002928">
        <property type="entry name" value="Myosin_tail"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014751">
        <property type="entry name" value="XRCC4-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46349">
        <property type="entry name" value="CINGULIN-LIKE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46349:SF6">
        <property type="entry name" value="MYOSIN-6-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01576">
        <property type="entry name" value="Myosin_tail_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90257">
        <property type="entry name" value="Myosin rod fragments"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0505">Motor protein</keyword>
    <keyword evidence="9" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="7" id="KW-0518">Myosin</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0787">Thick filament</keyword>
    <feature type="domain" description="Myosin tail" evidence="12">
        <location>
            <begin position="296"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="289"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="430"/>
            <end position="626"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004877-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="656" mass="75969" checksum="CE8446D0AEEEE89F" modified="2020-08-12" version="1">MAPLFALDNRCERRERLIPTQIYQNNYGHAMHYYQPMIDYLDTKSASAEQSPLPDPRCPYLPYTEERGLNRYHTGYFHFRPYSDREVVRLSEAAIDTAERYLPDYRFDIKRSVLSVTATADAARLKKHVPADSVIDRYNRKVAVRNAQHAADDKRDRMALTKRTHFYGNLKDSDSDLSPELKKAIKGKSADQIKNILLNESRRTRESEERSLNQISSRFSSSVSRQAVGSRANSEVRYIVKRSTSTVDVSQAAEEASTQYKAVHTSVDNVRREIKNFAVKTQEFLHDTSKQTSIEIEQLNARVVEAETKLKSEVQRIKKKLQIQITELEMSLDVANHTNIELQKTIKKQSLQLTEMQAHYEEIQRQLQSTVDQYGIAQRRIQSLSGELEEIRINYDSSLRAKRQVEVSLEEATTRINELSVVNANYASLKAKLETELQTLAVDYEEVTRELRVSDERYQKIQVELKHTVELLHEEQERIVKIEAIKKSLEVEVKQLSVRLEEVEVNAVAGSRRIINKLEARLRDIEVELDEERRKHAETIKILRKKERSVKEVYIQIEEDQKNIQILQDALEKANQKIAAYKRQLTEQEQSSQQCVTKVRRFQRELEAAEDRADVAESNLSLVRAKHRTFVTTSTVPGSQVYLVQETTRTINETSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VKG6</accession>
    <name>A0A6E8VKG6_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Homeotic protein antennapedia</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Sequence-specific transcription factor which is part of a developmental regulatory system that regulates segmental identity in the mesothorax. Provides cells with specific positional identities on the anterior-posterior axis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the Antp homeobox family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VKG6"/>
    <dbReference type="EnsemblMetazoa" id="ACON004660-RA">
        <property type="protein sequence ID" value="ACON004660-PA"/>
        <property type="gene ID" value="ACON004660"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002215"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004660"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038238"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009952">
        <property type="term" value="P:anterior/posterior pattern specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000017">
        <property type="entry name" value="Homeobox protein antennapedia"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050296">
        <property type="entry name" value="Antp_homeobox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017995">
        <property type="entry name" value="Homeobox_antennapedia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001827">
        <property type="entry name" value="Homeobox_Antennapedia_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45659">
        <property type="entry name" value="HOMEOBOX PROTEIN HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45659:SF22">
        <property type="entry name" value="HOMEOTIC PROTEIN ANTENNAPEDIA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00025">
        <property type="entry name" value="ANTENNAPEDIA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
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    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
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    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="58"/>
            <end position="118"/>
        </location>
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            <begin position="60"/>
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            <begin position="1"/>
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            <begin position="13"/>
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            <dbReference type="ARBA" id="ARBA00004123"/>
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            <dbReference type="PROSITE" id="PS50071"/>
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        <source>
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        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
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    </evidence>
    <sequence length="140" mass="16367" checksum="FC4883850971935A" modified="2020-08-12" version="1">MAMYTNAGGGPPGVTQQQPNMMHQQPPPLHQGQQAPPNSQNASSGLQSPLYPWMRSQFERKRGRQTYTRYQTLELEKEFHFNRYLTRRRRIEIAHALCLTERQIKIWFQNRRMKWKKENKTKGEPGSGDENDMTPPQSPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VMF3</accession>
    <name>A0A6E8VMF3_ANOCL</name>
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        <submittedName>
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        <name type="scientific">Anopheles coluzzii</name>
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        </lineage>
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        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain>Mali-NIH</strain>
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    <reference evidence="6" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="6">Ngousso</strain>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="280" mass="31871" checksum="FA0976200CEEED1E" modified="2023-06-28" version="2">MYQAARTVAASSSSRLHSPVAWSRKSVQEGYYDLEAGETERTPLLYVRDNGRYRAKSLHRANDPNRVHPVALGLMVALLVFLLIGVVIGVYLLLLTVQRPWPVSHPFFLVERPAWWQYPVALEAATLDRLAVTNVIVLHTDSEDCLDQERCVRYLQNTERSCWAARRDHIPYNFLIGGDGVTYEARGWKSQHGFVDLPGRNTTLVVGMIGNFTDRQPAEVQYAELKAFITESIRRLSLSPQYRLHGAVNATRPSRDGTIALYSQLERWPHWKGFVARDQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VMF7</accession>
    <name>A0A6E8VMF7_ANOCL</name>
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        <submittedName>
            <fullName evidence="3">IMD domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
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            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VMF7"/>
    <dbReference type="EnsemblMetazoa" id="ACON005508-RC">
        <property type="protein sequence ID" value="ACON005508-PC"/>
        <property type="gene ID" value="ACON005508"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011830"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005508"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038027"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
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    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:phospholipid binding"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:plasma membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013606">
        <property type="entry name" value="I-BAR_dom"/>
    </dbReference>
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        <property type="entry name" value="MTSS1/MTSS2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15708">
        <property type="entry name" value="ACTIN BUNDLING/MISSING IN METASTASIS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15708:SF4">
        <property type="entry name" value="FI21477P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08397">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="IMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
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            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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        </location>
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        </location>
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        </location>
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        </location>
    </feature>
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        </location>
    </feature>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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    </feature>
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        <location>
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    </feature>
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    </feature>
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        <location>
            <begin position="928"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51338"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1061" mass="113701" checksum="9C0D90BA57E2847F" modified="2020-08-12" version="1">MDIGMDRSGTDAGMATIGSLFQHIVNEMKNSSPLWEDFIAKATKLHACLRAAIQALAAYLDAFQKIADAATNSRGATKEIGTALTRVCLRHKAVESRMKTFTTAIMDCLVVPLQEKLEDWKKQVTVIDKDHAKEYKRCRAELKKRSSDTLRLQKKAKKGAADNLHVLVESSMQDVTMRRCELEEVERKSLRAIMVEERTRYCTFVNMLQPVVHEECEVMSELGHLQEAMQLIAIVTKDPAQLPQASEELILESKANISLYPDSPGGSNSQGGCSNSLGSRKSSVCSISSINSSSSGSPGHHQFQRSLSQASTLASQSHAVSTWPPHTQDATSTVDRPHTISSAYEKGHQRPALTVYTFQSPETIAETQKSPANVACRPPLPVRPLSTTSVKNANPNVPRQCPSPIPAHITKGMNEKCRISSPTYVNMTELASMAASKTTNNSNNVNNNNNNNNHHTSTSGNNGGASANNVQQAASLQSHSQIPYQQQQQQQQQLHSPVLSSASSLISPDSNATNAISSPDVSACSTQTPQNTPQTGSPGTPNYSSIGCAGGGTINLGFRSTTPSSAGGGTAAVTPSESNIDTSSNHTITIDDNEEHFNNTISTTSASANITSTTTISTSDTSNNSSSNNTTTNTTTTATSSTNKPSPPHCSTVGGTADAPPKSVLDKDKDILKRTGSVLEKTSLFEQQINNNQLHQPTVPPPQQQQLSVPTSPATLSTRHNDPNALYGRRTNEEIYKSAGQLLLDRDAGPTRVTRRASVNTVKPPPPVRRSSSVTPSPSVGTNSTNTTNTTNLAQQSLAYTSSESLPPPPAYLLDSAAGSSPSISGNVAGTVKALNEIRHTPASPGVLRRAQQQSNPPSNQGSPTQYTNQYGTLPANRQHGHDHIASTTSSLPPTAPTAAAVTAPQSHYPPSQHHHHHHPSTPNSFHATDSNKPLSNRSPKTNLHQQGGIYAQPKQLSTMSSFRTSSPGPQKPNSSFLAQLNAKIAPNKTPQSHAGFLENLNARLAEQRLSGKAFAVRNLINSKALPDPRICHESLMDQIKRGATLKRNRTINDRSAPKIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VMG9</accession>
    <name>A0A6E8VMG9_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Grh/CP2 DB domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VMG9"/>
    <dbReference type="EnsemblMetazoa" id="ACON005564-RB">
        <property type="protein sequence ID" value="ACON005564-PB"/>
        <property type="gene ID" value="ACON005564"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010710"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005564"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_029581"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007604">
        <property type="entry name" value="CP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057520">
        <property type="entry name" value="GRHL1/CP2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040167">
        <property type="entry name" value="TF_CP2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11037:SF20">
        <property type="entry name" value="PROTEIN GRAINYHEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11037">
        <property type="entry name" value="TRANSCRIPTION FACTOR CP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04516">
        <property type="entry name" value="CP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25416">
        <property type="entry name" value="GRHL1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51968">
        <property type="entry name" value="GRH_CP2_DB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Grh/CP2 DB" evidence="8">
        <location>
            <begin position="453"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="54"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="178"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="77"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="183"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="197"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="229"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="242"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005564-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="867" mass="96438" checksum="75FB89834A060854" modified="2023-06-28" version="2">MQTASHQTDSPMAGDPSPVHTGPDSSNIIHLATSSAANTPITYARYYDQQSPLGTGIIGGPGGHPVPSSPVEDTGVTDPSISPSLNLIQQHHQQQQQHEHHEHHHQQQQHQQEQDHHHHQQQQHQQIHQLQNNNGTFVYEYYKLPEKDALQWSTNAGAKLNGQTLQAMDLLLGAHPSSQAINVKREPEDLRKEPKNPRNQKVSSPSLHIKEPPPSPGSPSTETMYAAPGSTQIYLQGPSHQNSTAATASSNVNTNSPSPSSYAQYDMYPPNRLGPGGTTFITEPYTYREYFDNQGYAPARTIYGTAADSEGPQPATTYEGRFTKTGSIYTKTITSAGLTVDLPSPDSGIGADAITPRDQNNVQQQFDYAEPCQAPIGMVDPNAAGHIPACVASLQRNLAINGSQPSPTTSLGGSSTAAAVAVAGAAAAPRSRPWHDFGRQNDADKVQIPKIYTDVGFKYYLESPISSSQRREDDRITYINKGQFYGITLEYVHDPDKPLKNQTVKSVIMLLFREEKSPEDEIKAWQFWHSRQHSVKQRILDADTKNSVGLAGCIEEVSHNAIAVYWNPLESSAKINVAVQCLSTDFSSQKGVKGLPLHLQIDTFEDPRDTSVFHRGYCQIKVFCDKGAERKTRDEERRAAKRKMTATGRKKLDELYHPVVDRSEFYGMSDLMKPPVLFSPSEDIDKLTSMDMQFYGHDADSLSGTSDNVKSPFLLHANKPATPTLKFHNHFPPDVPTSDKKDPSIIMDGSMVTNSMVDFTPQIKRQRMTPPLSERVMLYVRQDNEDVYTPLHVVPPSTVGLLNAIENKFKISSSRINTIYRKNKKGITARIDDDMIRHYCNEDIFILEVQRYEEDLYDITLTELPTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VMI4</accession>
    <name>A0A6E8VMI4_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Phosphatidylinositol 3-kinase regulatory subunit alpha</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VMI4"/>
    <dbReference type="EnsemblMetazoa" id="ACON005583-RA">
        <property type="protein sequence ID" value="ACON005583-PA"/>
        <property type="gene ID" value="ACON005583"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004092"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005583"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035928"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005942">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046935">
        <property type="term" value="F:1-phosphatidylinositol-3-kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008286">
        <property type="term" value="P:insulin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12923">
        <property type="entry name" value="iSH2_PI3K_IA_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00159">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09942">
        <property type="entry name" value="SH2_nSH2_p85_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1490:FF:000026">
        <property type="entry name" value="AGAP005583-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000146">
        <property type="entry name" value="Phosphatidylinositol 3-kinase regulatory subunit alpha-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.555.10:FF:000070">
        <property type="entry name" value="Phosphatidylinositol 3-kinase regulatory subunit alpha-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000100">
        <property type="entry name" value="phosphatidylinositol 3-kinase regulatory subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000080">
        <property type="entry name" value="Pi3K21B, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032498">
        <property type="entry name" value="PI3K_P85_iSH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035022">
        <property type="entry name" value="PI3kinase_P85_nSH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10155">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3-KINASE REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10155:SF10">
        <property type="entry name" value="PI3K21B, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16454">
        <property type="entry name" value="PI3K_P85_iSH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07647">
        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00678">
        <property type="entry name" value="PI3KINASEP85"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="SAM" evidence="9">
        <location>
            <begin position="276"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="8">
        <location>
            <begin position="372"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="10">
        <location>
            <begin position="477"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="7">
        <location>
            <begin position="771"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="7">
        <location>
            <begin position="1052"/>
            <end position="1145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="433"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="684"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1148"/>
            <end position="1196"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="965"/>
            <end position="1006"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="725"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="1154"/>
            <end position="1179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005583-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1196" mass="133554" checksum="DD4E0BBB919B12C1" modified="2023-06-28" version="2">MEESQQTTASQAEQQQKEQQQQQQQQPSQPAPPPQQQQQGFGTYIALEDSTGPPTLVDELAFEKDDIVHVWMPTVTKPPTSTAAEGEGGPRVAWYKATNARTKQTGYVQLSKLSPLENIDPVVYSGGTARQYIGTVPAGTCVSIGCACVAGGLQQQQHQHQQQQQQQQQSQQTAISDAFGGLRLASSGTTPGTTDTTGSNAEELYVKCVTPATVLKDPGRDYIWGLGRQGGQCRNCSACFHLNCLPYTCYRPCQRNPDMYQNIPSTFRTEKSINEWTTENVLEWMTAVNMYDHVEVFKTKAIKGCDLPKLDRDKLEQMGIKNDFHQQSILTTITQLLMSAEMTAAAAAELPIVPAPVNLPVGTDQQQQQYNGHNLVNGSFSKQQKCDMCKNYLLGLVHQGLFCTQCNMVVHRQCSVMGLRPCSAAGTQQQQQSQQQSQQQSQQQQQQQQPTQQVGSTTGGYMLGGAYRQEHQHMFGKSLCLLFDVQQQAAPQIVVDLCTSLEKKAVMDKGLDLYVVYRTTPLRYDEVNKLRDALNENLLNIDLNGYTPECVATVLKKFFHELPDPLIPVAWYQSFIDASLLDDQQAVERMKGIIQEMAPHHNKTLQYFMRHLIRICRLQFMRGNKQQPTMLLQNWCHILMRPAWERIVHFVANIKNHLRVLELLMYKIDWNEALPEFLSIPAVPPRKTSRTGSGAGTGPGSTKKSIIASPEGGSIGGPLLESDYSTTTTGSGSSGNTLGRSAAQQYPNGGGMLMSLITDPNTPQELRDAEWYWGKISRDVAKEKMMDAPDGSFLVRDAINDAGEFTLVLKKDGTDRPIKIYHKNGKYGFTQECTFESLVGMINEFRTTTLKEYNTILDISLLYPISRFEDESLYPAAGDIHQLAASFYETVQKLTDLQNMRESTLEAFNVCQSHMELRRQAQEAFIEADKLFNDQLLTQARYEKEAQPHEQRSLAQNRTLIESRLEELRKCKKNLDEDMERERERMRELQREAMKLKPEIMNLSKQKDQYLEALKRQSITDAQIKQILKDGYLSTANPDGTQELPHLDETTWLRERYSRQDAEKKLANKPTGTFLIRARNAGHYALSIACDGRVNHCIIHQTERGYGFAEPYYIYDSLKSLVVHYATNSLEEHNDLLQTTLKYPAFAPNMPSGSGSGSGSGSGSGSGQTSGGGGGGGGSSSSRQQGSAPWSTSFAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VMI7</accession>
    <name>A0A6E8VMI7_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VMI7"/>
    <dbReference type="EnsemblMetazoa" id="ACON005614-RA">
        <property type="protein sequence ID" value="ACON005614-PA"/>
        <property type="gene ID" value="ACON005614"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039908353" evidence="1">
        <location>
            <begin position="17"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005614-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="177" mass="16591" checksum="D4DF9A2C01E11D71" modified="2023-06-28" version="2">MKLFVVLMALVAAAVAAPAPQFGFGYPGFGGGKFYRKALRRGFIPVAVPVPVAVAPIGVAPVGVLAPVGYVDPGPHYGGHYGGPVGGLLGGGGFSGSQANAQSASFNIGLGGISGSFSNSNANSFGGGPGGGFGGSGAQASAQSASFGGGLGGFGGSASNAQASANSFGGGGGGLFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VMI8</accession>
    <name>A0A6E8VMI8_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phospholipid scramblase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">May mediate accelerated ATP-independent bidirectional transbilayer migration of phospholipids upon binding calcium ions that results in a loss of phospholipid asymmetry in the plasma membrane.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the phospholipid scramblase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040229890.1">
        <property type="nucleotide sequence ID" value="XM_040373956.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VMI8"/>
    <dbReference type="EnsemblMetazoa" id="ACON005498-RC">
        <property type="protein sequence ID" value="ACON005498-PC"/>
        <property type="gene ID" value="ACON005498"/>
    </dbReference>
    <dbReference type="GeneID" id="120953738"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004238"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005498"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030082"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017128">
        <property type="term" value="F:phospholipid scramblase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005552">
        <property type="entry name" value="Scramblase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025659">
        <property type="entry name" value="Tubby-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23248:SF9">
        <property type="entry name" value="PHOSPHOLIPID SCRAMBLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23248">
        <property type="entry name" value="PHOSPHOLIPID SCRAMBLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03803">
        <property type="entry name" value="Scramblase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54518">
        <property type="entry name" value="Tubby C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0564">Palmitate</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU363116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005498-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="228" mass="25923" checksum="3E8A749B7D292334" modified="2020-08-12" version="1">MPIPQGIPNCPPGLEYLTAVDQLLVHQQVELLEAFTGFETANKYTVKNTLGQKVYWAVEDTTCCTRMCCGPDRPFDIKIMDNFQNEVLHLRRDLRCKSCCFPCCLQKLEVSAPPGNVIGTVVQKWSLCRPVFDIRDRNNETVLTIRGPVCQCPCSDIKFAVFTKDGTEVGKVTKQWTGFVQEHFTDADNFGINFPMDLDVRVKATMLGALFLIDYMYFESSDNKKVRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VP02</accession>
    <name>A0A6E8VP02_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Gustatory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Gustatory receptor which mediates acceptance or avoidance behavior, depending on its substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cell membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the insect chemoreceptor superfamily. Gustatory receptor (GR) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VP02"/>
    <dbReference type="EnsemblMetazoa" id="ACON006143-RE">
        <property type="protein sequence ID" value="ACON006143-PE"/>
        <property type="gene ID" value="ACON006143"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACON006143"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032593"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050909">
        <property type="term" value="P:sensory perception of taste"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013604">
        <property type="entry name" value="7TM_chemorcpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08395">
        <property type="entry name" value="7tm_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="65"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="129"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="162"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="235"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="268"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="348"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON006143-PE"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="373" mass="43479" checksum="B1A7CB35A3F87609" modified="2020-08-12" version="1">MHELQFLNIFNYCFVSPTYLHFDSLCNRYEFQHKNVFRNVTLLIVLVTCSVVSAIAVIAEFMQTSLLSVIGASSIILYTTRFLVMIPLTLWVWLCRHQLISDNNVALNVIQHKHAKFYPMPARRRTLQIYWIQVGSIVILLTAALAGQCITWWEIVVTRKEYFLLNVCALVALEILTLMHRMYIQCWALVIAHHLDELVGFVKNNRVELHLLRAAIVFWEELEFFKQRATSTFRVMNVLHVLDILITCVVETYTIFYVFEMGLSLVDALLNVITLTVYAATFFMFAYAHDLVKVKEAELKDALKSMQYTNLKRQSRDQKDFYDLVNLKLMMESPKITACGLFEINLQIFYNVFAAIITYIVILFQFRGFEKSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VP08</accession>
    <name>A0A6E8VP08_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_049462866.1">
        <property type="nucleotide sequence ID" value="XM_049606909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VP08"/>
    <dbReference type="EnsemblMetazoa" id="ACON006068-RC">
        <property type="protein sequence ID" value="ACON006068-PC"/>
        <property type="gene ID" value="ACON006068"/>
    </dbReference>
    <dbReference type="GeneID" id="120948157"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010612"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON006068"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039317"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16021">
        <property type="entry name" value="ALP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.720.10:FF:000017">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004245">
        <property type="entry name" value="DUF229"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10974:SF9">
        <property type="entry name" value="DUF229 DOMAIN CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10974">
        <property type="entry name" value="FI08016P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02995">
        <property type="entry name" value="DUF229"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON006068-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="713" mass="81675" checksum="CEFFE6FF05A6DCBA" modified="2020-08-12" version="1">MKPSVLQTLLEPATTALAVLSRKQQHHHRPSPTCTNNRRDAGGGGRVAGGLLHPVDRCGRAPVLAIGLLFCVTVYLYFMTGTDVTQMIVTIPADDADLPGPKVPKGFIVWGPGCQIMDLNPVAKDVMRLFSKERFVPCSPKQPLTSIEQHFDNDTVLVRYHRDQLDAFMPHYMRYVRCCYQSIERAGTKDKADKNFWVGECVPFNESFLLPPSVRNGVLVRCKAGVSESSKSKKAIYTNVHALIRPQPSVRERMDKFRGRTEKRPLSVLMIGIDSISRLNLIRAMPHTAQHLYDTGWFELKGYNKIDDNTYPNLMAILTGYNNSLAYSICNPKKVGQLEECPFMWKYFADSGYATAYAEDEAGINTFNYHKYGFVAQPTDHYFRPVALAAEKNLSKKMKNSLTFCLGYQNYADHIYQYALDFASQYRTEPYFGLFWTNTFSHNDISDPSSMDLRMKYYLEELDERGILNSSMVVFFSDHGLRFGPVRMLLTGWLEERLPFNFIWLPEWFREEHPEIVQALKINRNRLTNPYDLHATLKHVLELSGRIDNLPGPLSCPNCQSIFAEVPWNRSCEETAIESHWCTCANFQPIDKRSPMVQRAIAFVIDYVNRDLEEHRNSTTVNYACAKLAVGSITLAKMAHEPKGRLPYRDYLLIFDGKPGGGKFESTVRHYQERDAFEITGSISRLNEYASQSECMHIDYLRKYCYCQRTKLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VQP3</accession>
    <name>A0A6E8VQP3_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="3">PDZ domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VQP3"/>
    <dbReference type="EnsemblMetazoa" id="ACON006260-RD">
        <property type="protein sequence ID" value="ACON006260-PD"/>
        <property type="gene ID" value="ACON006260"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004821"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON006260"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032404"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031847">
        <property type="entry name" value="PDLI1-4/Zasp-like_mid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006643">
        <property type="entry name" value="Zasp-like_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15936">
        <property type="entry name" value="DUF4749"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00735">
        <property type="entry name" value="ZM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zasp-like motif" evidence="2">
        <location>
            <begin position="100"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="125"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="125"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON006260-PD"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="243" mass="27448" checksum="B2D7F663D1DAE78D" modified="2020-08-12" version="1">MAAAYQYRGSPAFIHFAHRNENYDEAQIDYQPYRTTPLVLPGAKVPKKDTLPTESYLRHHPNPAMRAPPAHDYTDSLMKQKLAETVIHRVIGEEPPSGPKVVHKQFNSPIGLYSDNNIENTIRQSAPQQTQTVPSNGYTNNRQHRPTKIEGYKKTVVFDPCKSETYRALQEGTGEGLQEVPNPIQPKTFAPNRLVPGKKPNANHPAPQPEFAYRVNSMGEPNEKIHQSGSFKRLMLHVMSEMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VR17</accession>
    <name>A0A6E8VR17_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="3">MADF domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VR17"/>
    <dbReference type="EnsemblMetazoa" id="ACON006800-RB">
        <property type="protein sequence ID" value="ACON006800-PB"/>
        <property type="gene ID" value="ACON006800"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_003452"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON006800"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041913"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006578">
        <property type="entry name" value="MADF-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039353">
        <property type="entry name" value="TF_Adf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12243:SF64">
        <property type="entry name" value="DORSAL INTERACTING PROTEIN 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12243">
        <property type="entry name" value="MADF DOMAIN TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10545">
        <property type="entry name" value="MADF_DNA_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00595">
        <property type="entry name" value="MADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51029">
        <property type="entry name" value="MADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MADF" evidence="2">
        <location>
            <begin position="161"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="315"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="479"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="39"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="108"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="325"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="479"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="514"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="533"/>
            <end position="555"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON006800-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="555" mass="61451" checksum="236E05B765789F5E" modified="2020-08-12" version="1">MMQQAVSIKAEPDWSDLLVAEPDNRAQYALENVIRQQQQQLLQQQQQQQQQQQQQQQQQQQHQQQQHHQQQQQQQQQQQQQQQQYHLRNGAGASLTPNTSNPVAIVNGAGAAGSSASQQSGGDPSEDNEPLDMIEAIGDSDDNDFAEAESLTLQQFKKDEKLCDYYRRMPVLYDKSHPHYKFQSKKEKAWRELSRLCEMDVEQCKKRMTYFRCRFTVERRIMKNGVNCSEWPLLEKLKFLNKHIKIRRPRAPNGEPDDTDDETNPMNILRKRQREQQEDAKDDLHAYLDLQQMAVAAQNSHAQLQYQQHLKLQGHGPFGGPPGAGFPSAHDPMGLHHLQPPHPHQRDHQLPPPHGATACRPVATIRTITRTDRAAAQPTPVESPIPAAMSNVEVSYGLPKRSRVGYDGDEASLSNFNLNRTVKYQNNLEHQAHLDEHGAYGLYVGEVLRKLPERISSLTSLKIMQLLYEAQVQSFDAAPSSAAATPKPSSQSKGAGGGAVGEPSNGPAGGGGAAPASSSSIGSLLANGGGATSVASSEGGRQSTESAESMSNNKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VR47</accession>
    <name>A0A6E8VR47_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phenoloxidase-activating factor 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Prophenoloxidase-activating factor II</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase S1 family. CLIP subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VR47"/>
    <dbReference type="EnsemblMetazoa" id="ACON006954-RB">
        <property type="protein sequence ID" value="ACON006954-PB"/>
        <property type="gene ID" value="ACON006954"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002731"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON006954"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035592"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000038">
        <property type="entry name" value="Serine protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24258:SF142">
        <property type="entry name" value="PEPTIDASE S1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24258">
        <property type="entry name" value="SERINE PROTEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026248617" description="Phenoloxidase-activating factor 2" evidence="8">
        <location>
            <begin position="22"/>
            <end position="977"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="9">
        <location>
            <begin position="731"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="135"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="576"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="140"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="155"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="581"/>
            <end position="606"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00068096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00076468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON006954-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="977" mass="104823" checksum="863EA7A1F116C916" modified="2020-08-12" version="1">MRLLGSAALLLVLSAVAVIGADEWGWLPLEDGLQGEEKVREKRQDIGVLPASQQNVTEVSDEDILKFILDSGRQGRSLEGFDEVYSDPSVQDALQNADDAQARNIIKDKLCSLGLMQCDGQGESIEGKRPYYPIYAQQQPPRPRPPVRGPPLPPQQYAQRPPQPPQQQAYPPKPQHQGPYGPPKPMPVPNHAQHKIGYAGPQQRPPYSGPPNSFAGPSYASAYPSKPLGPIYEGDTPPFEFEPIGAGEKIYESPLSKPGLVLNEGPLVKPVEQHIHHHYHHIDGAADSKTVVVNNPGPVGASVLSGSAVVGGSSSSSFNSGLYSAGGVYGGAQGLSGGGFSPLAGSDFDYKELKGVNNNNGFGQSGVYAAQSKPVFEGSSSSFGQGGQSSLYNQGPAQFGSNSLYSGESSSAYQGGNGAGFHSSNPALYKKELNVKGPAASNGLGTYSGNNYSKYSQYANGQYAANGGQFGSSGGQFGANGGQQFGANGGQQFGANGGQFGANGGQYSQFANGNGIEDCVCVPYDQCPAQDVIGRKDDLILPLDPRNLKTDIEAAADEVVITDGNGTMTVVRVPKNATAEPETKTKKISKREAAEGKSNEAKDKASIEPSVPTPAPGHAQGSPTISFPSSRRRRRATDVSVPESLEEGQETVKQIEEPVAVEPSSVQEKSFSTEKRQAYYGPRPGQQQQQCSGRQVCCRKPVYRNPASQNLGKCGVRNAQGINGRIKNPVYVDGDSEFGEYPWQVAILKKDPKESVYVCGGTLIDNLYIITAAHCVKTYNGFDLRVRLGEWDVNHDVEFYPYIERDIISVQVHPEYYAGTLDNDLAILKMDRPVDLTSAPHIAPACLPDKHTDFSGQRCWTTGWGKDAFGDYGKYQNILKEVDVPIVNHYQCQNQLRQTRLGYTYNLNQGFICAGGEEGKDACKGDGGGPLVCERNGVWQVVGVVSWGIGCGQANVPGVYVKVAHYLDWINQVRGRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VR49</accession>
    <name>A0A6E8VR49_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the filamin family.</text>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON007006-RA">
        <property type="protein sequence ID" value="ACON007006-PA"/>
        <property type="gene ID" value="ACON007006"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_013864"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007006"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031100"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001473">
        <property type="entry name" value="Jitterbug, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001349">
        <property type="entry name" value="Jitterbug, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001145">
        <property type="entry name" value="Jitterbug, isoform I"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001193">
        <property type="entry name" value="Jitterbug, isoform M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044801">
        <property type="entry name" value="Filamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017868">
        <property type="entry name" value="Filamin/ABP280_repeat-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001298">
        <property type="entry name" value="Filamin/ABP280_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38537">
        <property type="entry name" value="JITTERBUG, ISOFORM N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38537:SF13">
        <property type="entry name" value="JITTERBUG, ISOFORM N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00630">
        <property type="entry name" value="Filamin"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="SMART" id="SM00557">
        <property type="entry name" value="IG_FLMN"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50194">
        <property type="entry name" value="FILAMIN_REPEAT"/>
        <property type="match status" value="17"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="Filamin" evidence="3">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="3">
        <location>
            <begin position="86"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="3">
        <location>
            <begin position="181"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="3">
        <location>
            <begin position="284"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="3">
        <location>
            <begin position="368"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="3">
        <location>
            <begin position="464"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="3">
        <location>
            <begin position="606"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="3">
        <location>
            <begin position="722"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="3">
        <location>
            <begin position="811"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="3">
        <location>
            <begin position="969"/>
            <end position="1069"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="3">
        <location>
            <begin position="1070"/>
            <end position="1164"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="3">
        <location>
            <begin position="1162"/>
            <end position="1256"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="3">
        <location>
            <begin position="1257"/>
            <end position="1350"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="3">
        <location>
            <begin position="1351"/>
            <end position="1443"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="3">
        <location>
            <begin position="1445"/>
            <end position="1543"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="3">
        <location>
            <begin position="1544"/>
            <end position="1637"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="3">
        <location>
            <begin position="1638"/>
            <end position="1734"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007006-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1748" mass="185431" checksum="BB30B1011D8E8AD6" modified="2020-08-12" version="1">MSSPGLTALGESTRLVPANLPAVFEVLPPPGASIRGSDCVATVLSPSKTKVSARVTHESANGSLRIEFVPSEVGTHIIEASIGGTTLVGGPLIAKVYDSSLIQVTDVNGGVVGQPCQFRVDASAAGEGQLEISINEGEVPNHVQVVGGGRCLVSFTPEQAKPHLIDIKFNGETVIGCPFVCSVADTSRVLLNLTNLELIPVNRPASFHITVSGGGAAELAVSVRGPQGELPVRVTGDIHAGFTAEFTPNHVGAHTINVEYNGYPVQGTPFVAKSYDATKVAVGSVSKGTVGRPVQFTVDAGDAGEGNLEITISAKGHNIPTQVHPQGNAKFAVSFVPAEPCEHIINVSFNKMLVPGCPITVIINGGTTGPQVSLGGPGPLHMPNSLVINHAGGRLEDIEVNVEGPSGHSVPAQVLQTADGVFKAEFVPRVVGEHRVSVTVEGQPTVGSPYSAKVYDVTAIKVKNVNNGTVGKPVVFLVETSQAGPGNLEVTVNGGRVPTSAQAQGQHTYAISFTPREAQNHTVELRFNGQDVPGSPFTCKVSPAARIVSSDLTDKVSVGHTFDFVVESDIAPVVEVLGPARRPVRADIVPAASPSSGYRVKFEPVEVGDHSVEVRLPGSGHVEGSPFLLKAYSAEKVVVTDIRPGVVGKSVSFGINASQAGAGNLEIIVAVGGKNVPNFVQSEGNARFKVNFKPTEAATHSLSVRFNGYPVPGSPFACHVTHAPVSLSKAIATGECLRQAPVKAESVFELEGFDGIDPQVLITAPSGDTVTSRVSFRDEVHLVAFVPTSVGRHLISVTANDQHINGSPFSCNVFDVSRVSISGLDPRSTMASLGVPLTFSVDAAGAGEGTLELVVSTATSTVKAEVTACARGLYDVTFIPQSCEPHFVNITFNDLPVEGSPFRCEVQQNTQHVQVGNTTLIDLMADDQTVEIFDPENKLVPFTLSRKAAEFRAAKIGQYVVRYIDQETRNFIAARTINVFDPSMVKIVEVGEAYCHKPASLVVSTNEAGQGTLTSMVRCGGLEVPHSIRGTANGGVWEIVYHPTRVAPHKIMILYNQVPISNKAIEINVLAPAMSKEITVNGLGLYQARVGKTTSFAIDTVGHPAREFDVVVSGPGGQALPVRCYQTKGGHLQAEFTVQKVGQCLIDVLHQSKPLMGSPYTCESYDPTKIQLQKVPKMNLCANSPISWIVQSESAGVAEIEVTVLSPTGQNVPVKLTQQDDYEHSIEFVPQAAGHYKATVMYGGEAVPNSPITFAVQSAGGKSDSHASGNGLEVAHRGKETSFVVFCPTAPNVQIERTDEQAERIEPRIKNMGNNQWKIFYTILTVGRYEIRASCTNRGPLPGSPWNIACLDPAKVTPIGGWGSCLDSSGKLVLPSKIVFDTSMAGPGELTCYVDDAEVMVEKQGSGKCVLFLSDDGLAKGEHSFDLTWSGLPISQSPGFVYVTGMTIGSDRVVLTGRGLTTAQVGEVSHFTIDATDALSGKPEVHMAYDDGEPLPVTLLQPRPNELIWLASYNPHKSTGGPLSLEVEWNGRIIKGCPLTIPVGPAVDAAKVLCSGEGLRNGVVGREIKSWIDTRRAGPGELTAHCAGPRKVAYCELYDHGDATFTLNVKPQEPGRHTLTIKYGGQHVPGSPYTLRVVGAPDASKVRVYGPGIEHGVLATFQSRFIVDTRGAGAGQLTVRVRGPKGAFRVEMQRESQKDRTILCKYDPTEPGDYRVEVKWAGELVPGSPFPVLIFDTQEELKRFLHGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VSU1</accession>
    <name>A0A6E8VSU1_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ENTH domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the epsin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VSU1"/>
    <dbReference type="EnsemblMetazoa" id="ACON007396-RB">
        <property type="protein sequence ID" value="ACON007396-PB"/>
        <property type="gene ID" value="ACON007396"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON007396-RC">
        <property type="protein sequence ID" value="ACON007396-PC"/>
        <property type="gene ID" value="ACON007396"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON007396-RD">
        <property type="protein sequence ID" value="ACON007396-PD"/>
        <property type="gene ID" value="ACON007396"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004706"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007396"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038549"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030125">
        <property type="term" value="C:clathrin vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16990">
        <property type="entry name" value="ENTH_Epsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000002">
        <property type="entry name" value="epsin-2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003903">
        <property type="entry name" value="UIM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12276">
        <property type="entry name" value="EPSIN/ENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12276:SF115">
        <property type="entry name" value="FI19443P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01417">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00273">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00726">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50942">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50330">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ENTH" evidence="8">
        <location>
            <begin position="12"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="157"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="219"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="277"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="512"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="662"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="222"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="277"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="307"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="329"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="353"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="513"/>
            <end position="566"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007396-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="683" mass="71883" checksum="B206FC6B3B1CBA02" modified="2020-08-12" version="1">MNVAGIRRNIKNLAHNYSDAQVKVREATSNDPWGPSSTIMAEIADLTYNVVAFSEIMQMIWKRMNDHGKNWRHVYKALLLLEYLIKTGTEKVAQQCKENIYAIQTLKEFQYMEEGKDQGMHVREKAKQLVSLLKDDERLKNERARALKAKERFARTASGFGSDGSIDGPTQRDPRPPNWGEGEPIAGAATSGVGKPVSEIEFVRPQTVGEEELQLQLAMAMSREEAEQEEQKRRSDDVRLQLALSQSEQDFKKDGDPPAGSSALVDLLDISFGAASISSPSQQPLAGPSGTSGTIDPWGMPVDGGSRPTTTDPWSRTSSPPAVVDPWLNTASALPTAGPSTSKPPMLGADTWQPRTQSPSVTSGSSVEGWLQNGAGAGAAAAGPMMMNGAGASNGNLDPWYSKTNAVPIGATAPPLGVASGDPWQANKRSTPVPTAAAAAASGDPWQANASAVKLDPWAPVSNNSATGSVGDLEATFGTAGGPLMNRPSPVGAITSPVSDLDEFDIITKRAASNNSSGSTTNNNNNHNAASNNLNNNSSLLLGDLDPLSSSGTNSSSPSMGASTAAKKTPQSFLGENSALVNLDNLIKPMPSGGSVAAAATSSAAYNPFGETGGAGAPVQKNLFQQNQPQVPSINQLKQSPFPVTLNQDPWAPVANMTTAQANLNNNNSAPWMNPQSSNPFLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VSU5</accession>
    <name>A0A6E8VSU5_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Grh/CP2 DB domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the grh/CP2 family. CP2 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VSU5"/>
    <dbReference type="EnsemblMetazoa" id="ACON007065-RA">
        <property type="protein sequence ID" value="ACON007065-PA"/>
        <property type="gene ID" value="ACON007065"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004181"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007065"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_029286"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000073">
        <property type="entry name" value="Gemini, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007604">
        <property type="entry name" value="CP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041418">
        <property type="entry name" value="SAM_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040167">
        <property type="entry name" value="TF_CP2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11037:SF21">
        <property type="entry name" value="GEMINI, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11037">
        <property type="entry name" value="TRANSCRIPTION FACTOR CP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04516">
        <property type="entry name" value="CP2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18016">
        <property type="entry name" value="SAM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51968">
        <property type="entry name" value="GRH_CP2_DB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Grh/CP2 DB" evidence="4">
        <location>
            <begin position="263"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="26"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="94"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="26"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="110"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="148"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="179"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007065-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="876" mass="93112" checksum="BA29D49CCE376D28" modified="2020-08-12" version="1">MCETYGFGGDTMDKDYVRKRLKCFNNSNSNGNEIGNGNSLGPNAHTNNANNNGTANNNELGSMRWADDLDLDLSNELSSNGYISDSLLNFPVFKQELPSPPQKHSHHQQHSQQQQQQQQQQHQLHQQQQQSLHQQAAAAAAAAAAATAGSSQDHSQPSQHTTPSGLQPSLSGNVPNSHQQQQQQQVAQQQYHQQQQGQNSMNGPGLMNNATHQQAVGAIGAGLSNLQSFLQSNRLDTDPSGGILQMQQDGTLTAARGDKTHDDHNRFQYVLAAATSIATKNNEESLTYLNQGQSYEIKLKKLGDLSPFRGKILKSIIKICFHERRLQYMEREQMQLWQASRPGERILDVDIPLSYGLMQVQPTSSNLLNTIEVFWDPMKEVGVYVKVNCISTEFTPKKHGGEKGVPFRIQVETYIDTNGGLHNGGGGGGANATAVGAVDDKDGIRPLHAAACQIKVFKLKGADRKHKQDREKILKRPVAEQEKYQRSCDCTILTDITTDSVIQPLGGSFSPEHIKRNVSPLLTGGPTSPGQLNKFENIMSSVLGGGGVAPNGLSPGVKPTPTTTAATAAMVVAAAAAAVAGVQANHPIGISNVSSTNGLCKASPLGGEQPIGGVLSPQQPSELDDYVPTITKETSPGSLAQWLAVHRLSAYTKTFAQFSGSDLLRMSKEDLCKICGLADGIRMFNILHSKAITPRLTIYVSFEANIYHAIYLHSNTIPELVQNLSKIPGFLEAINALNTPNSSDGGLWNGGSTFGAAGRSSLNAGGVVAASSSGGAGGNGGGGLGGKFSTPGAISLSVPSPSSPPISSSCGIAKLQLLINGPNGIQVLLTEDVLNNVKDETLFQLELKSNGNILMKAVHSVTAAGGPDCSIDDDAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VSW6</accession>
    <name>A0A6E8VSW6_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Guanine nucleotide exchange factor tim</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="EnsemblMetazoa" id="ACON007452-RA">
        <property type="protein sequence ID" value="ACON007452-PA"/>
        <property type="gene ID" value="ACON007452"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000658"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007452"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037458"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01221">
        <property type="entry name" value="PH_ephexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11793">
        <property type="entry name" value="SH3_ephexin1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047271">
        <property type="entry name" value="Ephexin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047270">
        <property type="entry name" value="PH_ephexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12845:SF5">
        <property type="entry name" value="EPHEXIN, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12845">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="DH" evidence="5">
        <location>
            <begin position="876"/>
            <end position="1063"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="4">
        <location>
            <begin position="1239"/>
            <end position="1300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="69"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="145"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="207"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="337"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="547"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="642"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="716"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="787"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1338"/>
            <end position="1373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="27"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="78"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="175"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="268"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="288"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="370"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="414"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="459"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="498"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="516"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="556"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="608"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="646"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="676"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="716"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="809"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="1342"/>
            <end position="1373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007452-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1373" mass="148695" checksum="41613162ED3F75FD" modified="2020-08-12" version="1">MPPPSATQQQQQERKPCARMALGYVHSSVPLPSSSSSSSSPSAVGTVPRKRRPRAVGEVFVSSTSTVYLYDPNDPLAKQSTGDGESVSPSNNATTSPRHDDAQLCPVYENFATETGQRSFRAVSRSAVPVLPLTVVRVRQPERCVANGLDGEPPKPPEVPPRRKHAAARAAVVKPDAEESRSEKSDRFELQSVSTFLDDSDHHYESLYSIGSNDGRHQHQHDSNYTTLPSHAEGSPLSGNVTSGVNFHEHRAGGGGGGDASAAMLLPPDDEFDSFDSDDTDYEDEDDDRNRARTDSGVDIRNAKLPDPPPSSSQVYVLVQKIKNLGTLSEIAKSFHKLSKKKGTKGATTTAGAGGGTYENTVLGSKHAGTKTGSKGMSSSTSSKSFKLPPLPLVGGGGGVPKPPPEDYEGAAVGSPHSGSHPAPPGSSLLLPASSPSLSSASTTANRAAAATPLISSPVPEQSSSAGVRSTSESSNVSGNVPKAPGTAGFSPADGATRKKSKSTKSLRSKLRKSLVSDSTSLNIGSSFNGSRSTFYVTSADVDSGIFNGSDGCLNPSASSVSQSQEMPTKPAEDNRRKSIASATGTTGHGRPTIPPPPPPAEAGKRTAASSSVSPLLAASNSKSVSKKLGATSWYAECGVFKQPHSFGTGSDQSLSNGKGDGSISPRSSLPPKDGGPTGAGSGGGVGGGNDRTLTSNGHTSASSWYADIYQTSGASVASSSESSGVSTGGEGGPGDDHSHSMFVNEPLYQIYNAAKLESITRDIDAEISGRSEAELYDDGYEKIAERNRRRKTGGTGEQEGAEEGDEGGCSSSSGSSSTDGDDRGDDSAMHLRKPSRPTALELIEPHIGKLRTLWCEVPEVRNSEILATLTPTEKRLQEAKFEILTSEASYLKSLNLLRTHFVNHPAFRDTRILSSSERKTLFSSIIPVQECSDRLLCDLENCWQDNIMLLGLSHSIYKHAEKHFHVYVTYCEHQAKIDRTLKALKANKPEFARTLAALEADPVCCSLSLSSFLMLPMQRITRMRLLLDAVLQRCHPDDDDEFGSWESTFVLINRILTQCNDAAHRSEQLYEMELLSRQIEFPTNVRPLAIVPCGIGAAPSTMHRKLEKRGDLVHVLWRGDDAKLTFGKKFSKSNVYAFLFTDLLVLTKKKSDESYLVIDYCQRALLTVSSGDIVTGLPAKEMQTIGKNLIIMTLLENHEGKTIEMILSCPSETERERWLMVTEPPASENPDEKIYEQWDCPQVIAVHPYQALQPDELDLDIKDVVNVHRKMADGWYEGERIRDGAVGWFPSNYTKEIPSAHIRAKHIKQRHLLLTYTSKYIDTATKAHHHYHNYYHQPQHQQHHHHQLHHKQQHLQHQQQYHHHHQQQQSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VT30</accession>
    <name>A0A6E8VT30_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ras-related protein Rab</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">The small GTPases Rab are key regulators in vesicle trafficking.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Membrane</location>
            <topology evidence="6">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VT30"/>
    <dbReference type="EnsemblMetazoa" id="ACON007654-RB">
        <property type="protein sequence ID" value="ACON007654-PB"/>
        <property type="gene ID" value="ACON007654"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004658"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007654"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_029555"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045335">
        <property type="term" value="C:phagocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008333">
        <property type="term" value="P:endosome to lysosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090385">
        <property type="term" value="P:phagosome-lysosome fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04107">
        <property type="entry name" value="Rab32_Rab38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000222">
        <property type="entry name" value="RAB32, member RAS oncogene family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
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    <dbReference type="InterPro" id="IPR030697">
        <property type="entry name" value="Rab29/Rab38/Rab32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47981">
        <property type="entry name" value="RAB FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47981:SF39">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 6" id="KW-0636">Prenylation</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007654-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="283" mass="31297" checksum="82A4AE2CEDBCC2FA" modified="2020-08-12" version="1">MVTTVITAVAAARRHTNGSEDNCEDDGGPRLSGPLRKLRRQSSRLRNLLPLLGGTGGIGSGKPVEQATMATGQAEKREHLYKILVIGELGTGKTSFIKRYVHQFFSQNYRATIGVDFALKVLNWDQNTIIRLQLWDIAGQERFGNMTRVYYKEAVGAFIVFDVTRSATFDAVIKWKNDLDSKVQLPDGKPIPCILLANKSDQQKQGIVTTPAKLDEYVKEHGFAGWFETSAKENVNIEEAAKSLVNKILMNDKLLNTGEVIDSERFALVGGKSDTSQKKSCSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VT37</accession>
    <name>A0A6E8VT37_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="6">SHSP domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3 4">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_049463371.1">
        <property type="nucleotide sequence ID" value="XM_049607414.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VT37"/>
    <dbReference type="EnsemblMetazoa" id="ACON007160-RB">
        <property type="protein sequence ID" value="ACON007160-PB"/>
        <property type="gene ID" value="ACON007160"/>
    </dbReference>
    <dbReference type="GeneID" id="120951935"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004108"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007160"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033299"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06526">
        <property type="entry name" value="metazoan_ACD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055269">
        <property type="entry name" value="Alpha-crystallin/HSP_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001436">
        <property type="entry name" value="Alpha-crystallin/sHSP_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45640">
        <property type="entry name" value="HEAT SHOCK PROTEIN HSP-12.2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45640:SF34">
        <property type="entry name" value="PROTEIN LETHAL(2)ESSENTIAL FOR LIFE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036514">
        <property type="entry name" value="Sm_HSP_B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00299">
        <property type="entry name" value="ACRYSTALLIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="SHSP" evidence="5">
        <location>
            <begin position="57"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR036514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR036514-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007160-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="168" mass="19827" checksum="2CF763B595E0C77B" modified="2020-08-12" version="1">MSLVPVQYRTWWDDWDLPLYTRVLEKSITQEVLGTDDYWRGAPLAPLRWSSLYRPWRYFSLRDVGAKVDTDRDRFQIELDVHQFLPHEVTVRRTDKYVTIEGKHEEKRDEQGYVARQFSRRYLVPIGYDANLIVSSLSSDGVLTVTAPRIGLPAPKVENSRRCLTYYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VT39</accession>
    <name>A0A6E8VT39_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptidase M14 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">Ngousso</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the peptidase M14 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VT39"/>
    <dbReference type="EnsemblMetazoa" id="ACON007530-RB">
        <property type="protein sequence ID" value="ACON007530-PB"/>
        <property type="gene ID" value="ACON007530"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011661"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007530"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030557"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.3120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050821">
        <property type="entry name" value="Cytosolic_carboxypeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000834">
        <property type="entry name" value="Peptidase_M14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12756">
        <property type="entry name" value="CYTOSOLIC CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12756:SF12">
        <property type="entry name" value="CYTOSOLIC CARBOXYPEPTIDASE-LIKE PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00246">
        <property type="entry name" value="Peptidase_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52035">
        <property type="entry name" value="PEPTIDASE_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M14" evidence="5">
        <location>
            <begin position="405"/>
            <end position="1123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="89"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="285"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="596"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="677"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="733"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="875"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="303"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="596"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="653"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="680"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="733"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="878"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="3">
        <location>
            <position position="1071"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS52035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007530-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1172" mass="125624" checksum="353F341639848E98" modified="2020-08-12" version="1">MTTAMMMVDNFECAGYTFYSKFDSGNLGKVELVRCCEGLGIVGNTVSSGTSGVPVIGSGSSAIGPGSTSASAIVAATLGIGLHQQQQQQHHGLLSSVSGSGSAAATPGANAGGSLGMPIPSPTPHATENPLVEVEFNLWTRPDCAGTPYENQNRTWFHFAVTGGRPNQIVKFNVMNLNKQAKLFSQGMHPVTKVGPNGRWERIKDKPSYSITNDVFFISFLHKVPEVGGDTTRIYYAFTFPFTYTELMDQLSAFDRKFGRHPFELHKMAYDISQKYDSLPATAAEESKNISNLAPHRRKGKLSKMERVDRTSPESFDETARDGVGEGSPPGKEVLAEAAKSLQTVLGPGGAGGMVIDENTSESMQQITNLVNKVKIELPQGGSEMKQPAQQASSSASAMKAKLPSFHHQLNVASAAAVAANPSPEDDQKADPRDEIYYCRELLTHSIEHRRIELLTISSFHGIQTARETRLRNLFPDEAAQRCHTFREKKIVFISSRVHPGETPASFVLNGFLSMLLDRKSIVSQTLRRMYVFKIIPFLNPDGVYNGLYRSDTRGHNLNRVYISPCHETQPAIYAARKLIRYYHLGVDEVESYELEKEKEHAHGEGSKEAIGVVDPESATPAGEGSAPVDSSGGNVKMALKKESDSVEQDTPAASDSGFSEASSTSSLLTSKVASSTALPTQSSADSTGSPPAADSTPAAVVQPQSLVAAATTAAQVFTEKLIPELNPIVSEGTATTASSSNNLAPATVRGTAGGRSASSLSLKKSIHSVTAKVDTGKGGIGKSAHHKQHVLNKPQFLGATPGTLKFKEFLVHPHHHHHHHHHHHHHHHYLAGGGAAGGVAGTGGAMSAGGNAQGQVVATLHGGKMALGAFKKGSAGGKQQATSSSTSSLIGVGGAGRASGSGGTVLGDGGTTVGGKNRPQTPQTNAAQYSHYRSHRNEYLNPVMGGGGVGGVPRSLNISLDFLAQLDERHDRVVYEDRSNLFMYIDLHGHASKKGVFMYGNHLPSTVEAVECMLLPRLMSLNSQHFHFDACNFSERNMYYKGKRDGLSKEGSGRVAVYKSTGLIKSYTLECNYNTGKCVNILPPRGKEIVAKTTHTLVPPKYTPAVFEEVGKALGPSILDLTNSNPLSRLPNCEFRTLQGLRNALRIEIERGSSKARVTNKYGDIISYDWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VT58</accession>
    <name>A0A6E8VT58_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Gelsolin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VT58"/>
    <dbReference type="EnsemblMetazoa" id="ACON007181-RA">
        <property type="protein sequence ID" value="ACON007181-PA"/>
        <property type="gene ID" value="ACON007181"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009656"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007181"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_036288"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051014">
        <property type="term" value="P:actin filament severing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008154">
        <property type="term" value="P:actin polymerization or depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051016">
        <property type="term" value="P:barbed-end actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036180">
        <property type="entry name" value="Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007122">
        <property type="entry name" value="Villin/Gelsolin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11977:SF123">
        <property type="entry name" value="GELSOLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11977">
        <property type="entry name" value="VILLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00262">
        <property type="entry name" value="GEL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82754">
        <property type="entry name" value="C-terminal, gelsolin-like domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="165"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="670"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="165"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="193"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007181-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="727" mass="78845" checksum="623F120C530C44D2" modified="2020-08-12" version="1">MFFTVRRRCFTRQIHPPDPAKLLLRLLYRGVIGADSMMSTLKQNALIVAKSHESPVKNNSPRPFSRPADLEGGNISDRLGKIKTSSENWKNRVELSDATNFTVAGRLPATKSPKLPFIKSDTKQSPAMNVFRSVNPPQLGLSKSPSMMVSSVVTSSTVYGQQPPTAAAPPAFGARPTSVPAPQFSSPAPAMFQQQSQQQQQQQHRVVESLMKRSISVPGVPSGGERTFGGDLKTQQPVGSKVAIPKLDDESFGSFFGKVANGATSTATTGSFSSSLTSSSSMMSSSSSAAEVTFGDLDTLKTSGEKLTQKKVVQGPRGRRAMSRNPLKTLAARDDLQTEYTEIRTGIADKEMRRLKLEAIAKSSNLAVEALAGLASVEDFKSVSLKSSSLPLNQSFVPHKPLMLLQVKGRRHAQTRLVEPAARSVNRGDCFILVTADRLFAFLGQYANVIERSRCKEICEQIVRDKDLGCSAASVTLLNDGGATAGRQARDFWALLGRGQEDEGVCDSGHADEDELFEACLIETNMVYEYGEESLVPVEEYWGAIPKIAMLDPRKVLVFDFGSELYVWSGKSAPAESKRAALRLAQELYGGGYSYEECQLSPLNFAELAGDRQRDARRLSKAGNSRPEWCLIARVTAHGDGAVPREVPRLAGRDGAVPRRVRRGWSVPDRARDTAAGRPGAVRARRTVPGAEPGAGKLQPRAGRLLLRPGLDAPLRCAHHVRDAVGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VT61</accession>
    <name>A0A6E8VT61_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="19">G_PROTEIN_RECEP_F1_2 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VT61"/>
    <dbReference type="EnsemblMetazoa" id="ACON007548-RA">
        <property type="protein sequence ID" value="ACON007548-PA"/>
        <property type="gene ID" value="ACON007548"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005495"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007548"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032315"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009881">
        <property type="term" value="F:photoreceptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007602">
        <property type="term" value="P:phototransduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050125">
        <property type="entry name" value="GPCR_opsins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027430">
        <property type="entry name" value="Retinal_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24240">
        <property type="entry name" value="OPSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00238">
        <property type="entry name" value="OPSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0157">Chromophore</keyword>
    <keyword evidence="9 15" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0600">Photoreceptor protein</keyword>
    <keyword evidence="11 15" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0681">Retinal protein</keyword>
    <keyword evidence="4" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="13 15" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 15 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="14" id="KW-0844">Vision</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="25"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="116"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="163"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="202"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="18">
        <location>
            <begin position="1"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="244"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="16">
        <location>
            <begin position="261"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="326"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007548-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="341" mass="36158" checksum="412812BEE8E347DE" modified="2020-08-12" version="1">MMEAPMFIYNSIHQGPALGSIGCTVYALMGAVGGTVAIATLTVISIDRYNVVVYPLNPNRSTTKLKCYFLIAFTWAYGLLFASFPALEIGLSRYTAEGYLTACSFDYLDRTYKARVFMFVYFVFAWLIPFAIISYCYARILIAVINANAIQSSKSKNKTEVKLAGVVVGIIGLWFAAWTPYAVVAMMGVFGYEQYLTPLNSMIPAVFAKIAASIDPYFYAMNHPRYRQMLERMFCNRGADQGNSQYQTSHYTRGASRGGDSEGGGGEESGGGGGGVGRAPGGGNAGLGRGGTVRGGGGRLIAGKGGGGANATGSNGGGGVKALKKQISNGDETSLEVSLEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VT62</accession>
    <name>A0A6E8VT62_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="9">SH3 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VT62"/>
    <dbReference type="EnsemblMetazoa" id="ACON007717-RB">
        <property type="protein sequence ID" value="ACON007717-PB"/>
        <property type="gene ID" value="ACON007717"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_008198"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007717"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032226"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041602"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070161">
        <property type="term" value="C:anchoring junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd11781">
        <property type="entry name" value="SH3_Sorbs_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11782">
        <property type="entry name" value="SH3_Sorbs_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11780">
        <property type="entry name" value="SH3_Sorbs_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000001">
        <property type="entry name" value="Sorbin and SH3 domain-containing protein 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050384">
        <property type="entry name" value="Endophilin_SH3RF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003127">
        <property type="entry name" value="SoHo_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14167:SF116">
        <property type="entry name" value="CAP, ISOFORM AC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14167">
        <property type="entry name" value="SH3 DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00499">
        <property type="entry name" value="P67PHOX"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00459">
        <property type="entry name" value="Sorb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50831">
        <property type="entry name" value="SOHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0965">Cell junction</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SoHo" evidence="8">
        <location>
            <begin position="254"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="7">
        <location>
            <begin position="593"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="7">
        <location>
            <begin position="666"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="7">
        <location>
            <begin position="791"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="18"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="35"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="47"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="141"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="184"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="197"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="208"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="238"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="249"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007717-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="850" mass="96251" checksum="655BFCBE37BD4CDB" modified="2020-08-12" version="1">MGQESGTTLTIGPKFKAKTSSLGVWSPFNRSTEVLTKEELAERSSSKNKSNTSQQSPVWTPRSAPPSPATERREFRPIGFESPKPTRRTLTPSPADPSTVVAPPWNQPGYNPPLPISDNFKPYPIQNSASNPTLGAPVRSASASTTSQGQVTQVRFPPQQKSTISHDPTINTLLKSKDGKTATIKASETSESQQKGSTTSIIRTSSSLMTNSSRQSSAGIINPTANQKPLVGQRSEKASTSLQDNNYSQDRKMQQTAHKVDGIGPITREGMPLTLRSEIDEGNRDKWYKQMYQTLHKTQDDADYVTVRYKTRRGYPYKSSGYQSEPEPNYDSDYTVKYSTLDRRRTPIGLSSTSYSKFNTLQNKPTTPQQYHQQSIKSVHSSYKNQPGKIEDYIPGRSSISEKESKEWWDEVMDIFNGQLEHQKLTTSKTYTEGNLSRALKEQGYESDSTLVFRKRDPPASAALSPVEQKQYYKNMQAGGEVPLQGFRKPAPERPKESPRRYIESDVNIHYKTPIRFEYKEPIPDDELAYRQAEHMRRVYQEERRRKYIHELEDMNARRHMDNILPSQKSPIPLNRYDDFPADLSPKPLIVQQPKTIARALYNFQGQSVRELSFRKGDIIYLRRQIDKNWYEGEHNATVGLLPANYIEILTRDNVNVKQPLPKKPTREGKARAKFNFTAQTAVELSLLKGELVTLTRRVDDNWFEGKIGSKKGIFPVSYVEILTDIDGEESYDIEPIVSRPQSALGVTSQTSQALTTHFDNSHTNGRISPGIVRETKTIQKTEVLHVDTSNEPISYRALYNYKPQNTDELELLEGDVVYVLEKCDDGWYVGTSARTGCFGTFPGNYVNKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VT64</accession>
    <name>A0A6E8VT64_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="5">V-SNARE coiled-coil homology domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VT64"/>
    <dbReference type="EnsemblMetazoa" id="ACON007721-RA">
        <property type="protein sequence ID" value="ACON007721-PA"/>
        <property type="gene ID" value="ACON007721"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON007721-RC">
        <property type="protein sequence ID" value="ACON007721-PC"/>
        <property type="gene ID" value="ACON007721"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000027"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007721"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_029877"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15870">
        <property type="entry name" value="R-SNARE_VAMP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001388">
        <property type="entry name" value="Synaptobrevin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016444">
        <property type="entry name" value="Synaptobrevin/VAMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042855">
        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45701">
        <property type="entry name" value="SYNAPTOBREVIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00957">
        <property type="entry name" value="Synaptobrevin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005409">
        <property type="entry name" value="Synaptobrevin_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00219">
        <property type="entry name" value="SYNAPTOBREVN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00417">
        <property type="entry name" value="SYNAPTOBREVIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50892">
        <property type="entry name" value="V_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="91"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="V-SNARE coiled-coil homology" evidence="4">
        <location>
            <begin position="27"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="15"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007721-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="112" mass="12363" checksum="87B40404F2CDE7A4" modified="2020-08-12" version="1">MSTPEAGNSNGTQPAGANASNNATTNRLKQTQAQVDEVVGIMRVNVEKVLERDQKLSELDQRADALQHGASQFEQQAGKLKRKQWWANMKMMIIMGVIGVVLLIIIILSIIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VT69</accession>
    <name>A0A6E8VT69_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">glycogenin glucosyltransferase</fullName>
            <ecNumber evidence="2">2.4.1.186</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 8 family. Glycogenin subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.186" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8VT69"/>
    <dbReference type="EnsemblMetazoa" id="ACON007724-RA">
        <property type="protein sequence ID" value="ACON007724-PA"/>
        <property type="gene ID" value="ACON007724"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_008034"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007724"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039185"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008466">
        <property type="term" value="F:glycogenin glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02537">
        <property type="entry name" value="GT8_Glycogenin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000085">
        <property type="entry name" value="Glycogenin, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002495">
        <property type="entry name" value="Glyco_trans_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050587">
        <property type="entry name" value="GNT1/Glycosyltrans_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11183">
        <property type="entry name" value="GLYCOGENIN SUBFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01501">
        <property type="entry name" value="Glyco_transf_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007724-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="321" mass="36651" checksum="C8AE61433C9E934D" modified="2020-08-12" version="1">MSKYAWVTLATNDSYSLGALVVAHSLKRVHTEHQTAVLITPGVSESMKTKLRAVFNVVEEVNLLDSKDEANLALLKRPELGVTFTKLHCWRLTQFEKCVFLDADTLVLRNCDELFEREELSAAPDIGWPDCFNSGVYVYTPNMETFSSLVQYAVTHGSFDGGDQGLLNSYFSDWAHKDIQKHLPFIYNTSSVATYSYLPAFKQFGQNTKILHFIGVAKPWLQNFNSETRKVYVPSECQHLANFLQYWWDIFAEDVHSRLSPDMSGLAGALANVTLGEPRTSEQDRIEEKLRRQYWEAGNAEYVGRDAFKHIWAKIQQTMDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VUV3</accession>
    <name>A0A6E8VUV3_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Protein pinocchio</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_040239954.1">
        <property type="nucleotide sequence ID" value="XM_040384020.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VUV3"/>
    <dbReference type="EnsemblMetazoa" id="ACON007751-RA">
        <property type="protein sequence ID" value="ACON007751-PA"/>
        <property type="gene ID" value="ACON007751"/>
    </dbReference>
    <dbReference type="GeneID" id="120960080"/>
    <dbReference type="CTD" id="33288"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000467"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007751"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030373"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007751-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="188" mass="19917" checksum="2997D202E29FD77A" modified="2020-08-12" version="1">MSVASVQAPHLADLCSSLSRTSLSMSSSLSDLVGSPSLGAVFDFHSSDAVLTIEELREQLGSCYTCGVSWTEDQVSLDCTECGGYSLERPCLICEGICGQLWKRDFTMSHACGKSRWQGVCTKFPAQMIQLQSSAPLSSCASFGSHHQLTGCAAAAAANNGASTASQQHFLQQELCARLEKLSANAHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VUV8</accession>
    <name>A0A6E8VUV8_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Tyrosine-protein kinase</fullName>
            <ecNumber evidence="13">2.7.10.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 13">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the protein kinase superfamily. Tyr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.2" evidence="13"/>
    <dbReference type="RefSeq" id="XP_040237120.1">
        <property type="nucleotide sequence ID" value="XM_040381186.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VUV8"/>
    <dbReference type="EnsemblMetazoa" id="ACON008317-RC">
        <property type="protein sequence ID" value="ACON008317-PC"/>
        <property type="gene ID" value="ACON008317"/>
    </dbReference>
    <dbReference type="GeneID" id="120958406"/>
    <dbReference type="CTD" id="124191448"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002310"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008317"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035633"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004715">
        <property type="term" value="F:non-membrane spanning protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048468">
        <property type="term" value="P:cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002009">
        <property type="term" value="P:morphogenesis of an epithelium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007424">
        <property type="term" value="P:open tracheal system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007435">
        <property type="term" value="P:salivary gland morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05059">
        <property type="entry name" value="PTKc_Tec_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09934">
        <property type="entry name" value="SH2_Tec_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11768">
        <property type="entry name" value="SH3_Tec_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000052">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000190">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000053">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000045">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050198">
        <property type="entry name" value="Non-receptor_tyrosine_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24418">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="1 11" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="3 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 13" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="SH3" evidence="16">
        <location>
            <begin position="235"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="15">
        <location>
            <begin position="303"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="419"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="43"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="66"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="177"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="447"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00051245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU362096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008317-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="679" mass="76248" checksum="7D591C21011B93A5" modified="2020-08-12" version="1">MIPCVSLTESSVLGNMKERVKEMRVFGCRLNFWNNVTLNFGSNKGTGSSPAQNSTRSTSPNVPNGHLPLPQQQSQVHPQQQPQQQQLQHHSGTVPVLQAHQQQQQQPQPTQHPPQQHSQSHSPSLQHPAASQQQVHLQQQQAALQAALQQQQQPPLLQQQTSQQSSHHLNSSSSSLGNITPTFVAQPQSPTVNSFKTASLNGVGLHVNGGGDLNMPGGPTVTPGTPNTKAKDVSPRVKIVVALYPFKAIESGDLSLEKNAEYEVIDDSQEHWWKVKDQHGTIGYIPSNYVKEKELLGLQKYDWYVGDMSRQRAESLLKQNDKEGCFVVRKSSTKGLYTLSLHTKVPQSHVKHYHIKQNARSEFYLSEKHCCETIPDLINYHRHNSGGLACRLKTSPCDRPVPPTAGLSHDKWEIHPSELMILEELGSGQFGVVRRAKWRGSIDTAVKMMKVGTMSEDDFIEEAKVMTKLQHPNLVQLYGVCSKHRPIYIITEYMKHGSLLNYLRRHEQSLIGNMGLLLDMCIQVCKGMSYLERHNYIHRDLAARNCLVGSENTVKVADFGLARYVLDDQYTSSGGTKFPIKWAPPEVLNYTRFSSKSDVWAYGVLMWEVFTCGKMPYGRLKNTEVVERVQRGIILEKPKACAKEIYDVMKKCWSHSPEDRPGFRILKDQLAAVAQGLTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VUX3</accession>
    <name>A0A6E8VUX3_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Fuseless</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_040231689.1">
        <property type="nucleotide sequence ID" value="XM_040375755.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VUX3"/>
    <dbReference type="EnsemblMetazoa" id="ACON008310-RA">
        <property type="protein sequence ID" value="ACON008310-PA"/>
        <property type="gene ID" value="ACON008310"/>
    </dbReference>
    <dbReference type="GeneID" id="120955171"/>
    <dbReference type="CTD" id="33765"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005253"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008310"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031613"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042734">
        <property type="term" value="C:presynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070073">
        <property type="term" value="P:clustering of voltage-gated calcium channels"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007274">
        <property type="term" value="P:neuromuscular synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007270">
        <property type="term" value="P:neuron-neuron synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032751">
        <property type="entry name" value="Fuseless"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35270">
        <property type="entry name" value="FUSELESS, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35270:SF2">
        <property type="entry name" value="FUSELESS, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15993">
        <property type="entry name" value="Fuseless"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="29"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="69"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="135"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="190"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="230"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="260"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="303"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="375"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="387"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="398"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008310-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="444" mass="50349" checksum="4CE80A66A5878E9B" modified="2020-08-12" version="1">MRGSIAGIEDGIIGTAQFRPSYHGLLLELIDYLYASLVVAPLVVCYWRGTWNLMGEYIYPSDLTTSLVVSLAIGIVGHIVFNIFQATLRQYLNADRHRIAYYLGSRLYTVIYGVVCVNGWRGGWQLIDLYTTHDVLYVVLITLGCLFILACLKGVRNVMGTPFLIINDSRREYFDVPTYFKLTGSKDPGLYVLDCMFSVLVIGSLVVFVWRGLWVLLDLKLFPEDHGTSAWASIIIGYGVTGVTFSLQPLMRWTCDRLTGFWRVVVADLFLFFSFIGTINVWRGIWQALDTYFLPDDPLLSNWITHGAALLLLILLNCSNSVLVRGVYIDAEEPAGQCVVFPVYYIRLFFQKERKKKQRRLFDALERADLNSTGGVLQDKKHHHSLQTAAQQPAQQKEQQKEAHQHHIAADQNHQVIRNLEAVPLTIIDDPKSNGDHSSQPTRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VV70</accession>
    <name>A0A6E8VV70_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">dual-specificity kinase</fullName>
            <ecNumber evidence="2">2.7.12.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.12.1" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8VV70"/>
    <dbReference type="EnsemblMetazoa" id="ACON007847-RA">
        <property type="protein sequence ID" value="ACON007847-PA"/>
        <property type="gene ID" value="ACON007847"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_013762"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007847"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038313"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004712">
        <property type="term" value="F:protein serine/threonine/tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000112">
        <property type="entry name" value="Putative dual specificity tyrosine-phosphorylation-regulated kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000127">
        <property type="entry name" value="Putative dual specificity tyrosine-phosphorylation-regulated kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.10.30">
        <property type="entry name" value="DYRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042521">
        <property type="entry name" value="DYRK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050494">
        <property type="entry name" value="Ser_Thr_dual-spec_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058:SF22">
        <property type="entry name" value="DUAL SPECIFICITY TYROSINE-PHOSPHORYLATION-REGULATED KINASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="203"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="104"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="521"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="110"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00051680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007847-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="558" mass="63868" checksum="A4B0635726626847" modified="2020-08-12" version="1">MVKMSRKSIRQKLLSSSCLCLLPQKQQSIKDVGEKLLPAPPPPPSTKIQLDSGKCSRERVQTTQQLYEANRPELAGLSGTRNVFRAGRSSNGTGTNMIDAHGNALPPLSPSSNNNTTTELENDKPMLPMTPASKYKTIEALDYHGVHLTDFEKQEIEKYKEIYYLGLDARKINAKPGVGANSGFDDENGCYQKIVHDHICYRYEILEIIGKGSFGQVIRALDHKTKQYVAIKIIRNKKRFHHQALVEVRILDELRKKDADGSHHVIHMLDYFYFRNHLCITFELMSLNLYELIKKNNYQGFSLSLIKRFCNSIVKCLRFLDELDIIHCDLKPENVLLKQRGSSTIKVIDFGSSCFSDRTVYTYIQSRFYRSPEVILGYPYDKAIDMWSLGCILAELYTGYPLFPGENEVEQLACIMEILGVPSKEVFQLATRKRLFFDSRGVPRCITNSKGRKRKPGSKTLSQALRCNDTVFIDFVSKCLEWDPHKRMTPEQAARHEWLQVSASKAYFGSKSKESSDTQLNFLHKQQRSQPMTPNTILPEIKTPSNRTLRFTKERVKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VV93</accession>
    <name>A0A6E8VV93_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VV93"/>
    <dbReference type="EnsemblMetazoa" id="ACON008443-RA">
        <property type="protein sequence ID" value="ACON008443-PA"/>
        <property type="gene ID" value="ACON008443"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_008820"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008443"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031299"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="241"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="324"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="162"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="241"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="269"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="294"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008443-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="433" mass="50370" checksum="1DA56004F2D75E19" modified="2020-08-12" version="1">MDASNIVGDDIDTKDCWWERPSVIYAVNLPRVTAQLEKLMGTNVVRLTDRKYMQELQRRIQEDYNVTLEKRISEREHREWQRQKQLILDGKYSLENCPRALIPLHSIVLAKEIERERNLILSGAGDSIPEEMTSSVFLVNQKTNVNILNKREKLKTIREKNAERLIKQGHRWERERQQMEEQSREREIERRTKREALLEQEKLYMIEENDRSLNLLRMKTKKGPSRDMNLRSMLMQQREELGRTIQKRDFDMKDERSGLGMEMEGGKGDRRRHRRGARKQRPMQRQNEVHEYQESSSPEEGYISMNNADTPEDLETSIAIKVGKGASKDSGDESEAEQDSDQAAVDEALVEQPNDANAGDDEEVAAEVDALEQQLEQQELTEAELEAAEEARLREELDKTLMEKKKARQVRNEDPKITKLKEADVSGRVGVSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VWZ2</accession>
    <name>A0A6E8VWZ2_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ras-related protein Rab-14</fullName>
            <ecNumber evidence="7">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="24">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasmic vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Early endosome membrane</location>
            <topology evidence="4">Lipid-anchor</topology>
            <orientation evidence="4">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus</location>
            <location evidence="3">trans-Golgi network membrane</location>
            <topology evidence="3">Lipid-anchor</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Recycling endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="7"/>
    <dbReference type="RefSeq" id="XP_040231880.1">
        <property type="nucleotide sequence ID" value="XM_040375946.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040231881.1">
        <property type="nucleotide sequence ID" value="XM_040375947.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_049464132.1">
        <property type="nucleotide sequence ID" value="XM_049608175.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VWZ2"/>
    <dbReference type="EnsemblMetazoa" id="ACON008419-RB">
        <property type="protein sequence ID" value="ACON008419-PB"/>
        <property type="gene ID" value="ACON008419"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON008419-RC">
        <property type="protein sequence ID" value="ACON008419-PC"/>
        <property type="gene ID" value="ACON008419"/>
    </dbReference>
    <dbReference type="GeneID" id="120955251"/>
    <dbReference type="CTD" id="51552"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004685"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008419"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042447"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045335">
        <property type="term" value="C:phagocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055037">
        <property type="term" value="C:recycling endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006895">
        <property type="term" value="P:Golgi to endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090382">
        <property type="term" value="P:phagosome maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04122">
        <property type="entry name" value="Rab14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000344">
        <property type="entry name" value="Ras-related protein Rab-14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030702">
        <property type="entry name" value="Rab14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0007">Acetylation</keyword>
    <keyword evidence="21" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="12" id="KW-0967">Endosome</keyword>
    <keyword evidence="16" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="17" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="19" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="18" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0488">Methylation</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-0636">Prenylation</keyword>
    <keyword evidence="14" id="KW-0653">Protein transport</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="185"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="198"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00014884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008419-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="215" mass="24191" checksum="06993A9C5E87CE34" modified="2020-08-12" version="1">MSAGPYNYSYIFKYIIIGDMGVGKSCLLHQFTEKKFMASCPHTIGVEFGTRIIEVDKQKIKLQIWDTAGQERFRAVTRSYYRGAAGALMVYDITRRSTYNHLSSWLTDTRNLTNPSTVIFLIGNKSDLESTREVTYEEAKNFADEHGLLFAEASAMTGQNVEEAFLETARKIYQSIKDGRLDLNSSETGVQHKPAQPGRTTLSNDAQTNKENCSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VWZ6</accession>
    <name>A0A6E8VWZ6_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Aromatic-L-amino-acid decarboxylase</fullName>
            <ecNumber evidence="8">4.1.1.28</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">DOPA decarboxylase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">Ngousso</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 11 12">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the group II decarboxylase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.28" evidence="8"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8VWZ6"/>
    <dbReference type="EnsemblMetazoa" id="ACON009091-RA">
        <property type="protein sequence ID" value="ACON009091-PA"/>
        <property type="gene ID" value="ACON009091"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005256"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON009091"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031052"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004058">
        <property type="term" value="F:aromatic-L-amino-acid decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042423">
        <property type="term" value="P:catecholamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042427">
        <property type="term" value="P:serotonin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06450">
        <property type="entry name" value="DOPA_deC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1340.10:FF:000001">
        <property type="entry name" value="Histidine decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000025">
        <property type="entry name" value="Histidine decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1340.10">
        <property type="entry name" value="dopa decarboxylase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010977">
        <property type="entry name" value="Aromatic_deC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002129">
        <property type="entry name" value="PyrdxlP-dep_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021115">
        <property type="entry name" value="Pyridoxal-P_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11999:SF167">
        <property type="entry name" value="AROMATIC-L-AMINO-ACID DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11999">
        <property type="entry name" value="GROUP II PYRIDOXAL-5-PHOSPHATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00282">
        <property type="entry name" value="Pyridoxal_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00800">
        <property type="entry name" value="YHDCRBOXLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00392">
        <property type="entry name" value="DDC_GAD_HDC_YDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0127">Catecholamine biosynthesis</keyword>
    <keyword evidence="5" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="7 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="6 11 12" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="11">
        <location>
            <position position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00040968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00041275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR602129-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009091-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="478" mass="53796" checksum="81464208259DB1DF" modified="2020-08-12" version="1">MQAPEFKDFAKEMVDYISNYLENIRDRRVLPTVQPGYLRPLIPDEAPPQPEKWEEVMADVERVIMPGVTHWHSPKFHAYFPTANSYPAIVADMLSGAIACIGFTWIASPACTELEVVMLDWLGKMLDLPKEFLACSGGQGGGVIQGTASEATLVALLGAKAKAMKRVKEEHPDWDDNTIVSKLVGYTSNQSHSSVERAGLLGGVKLRGLKADENLKLRGETLEQAIKEDLDAGLIPFYVVATLGTTNTCAFDRLDEIGPVANQHNVWVHVDAAYAGSAFICPEYRYLMKGIETADSFNFNPHKWMLVNFDCSAMWLKEPYWIVNAFNVDPLYLKHDMQGSAPDYRHWQIPLGRRFRALKLWFVLRLYGVDNLQAHIRRHCGFAKQFEALCRADDRFEIFGEVAMGLACFRLKGTNELSEALLKRINGRGNIHLVPSKVNDVYFLRMAVCSRFTEPADIDYSWKEVAAAADELLAEQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VWZ9</accession>
    <name>A0A6E8VWZ9_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase</fullName>
            <ecNumber evidence="6">3.1.4.11</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + H2O = 1D-myo-inositol 1,4,5-trisphosphate + a 1,2-diacyl-sn-glycerol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:33179"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
            <dbReference type="ChEBI" id="CHEBI:203600"/>
            <dbReference type="EC" id="3.1.4.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="8">Binds 1 Ca(2+) ion per subunit.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.11" evidence="6"/>
    <dbReference type="EnsemblMetazoa" id="ACON009115-RB">
        <property type="protein sequence ID" value="ACON009115-PB"/>
        <property type="gene ID" value="ACON009115"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012012"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON009115"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038989"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004435">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate phospholipase C activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008344">
        <property type="term" value="P:adult locomotory behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043153">
        <property type="term" value="P:entrainment of circadian clock by photoperiod"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042592">
        <property type="term" value="P:homeostatic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046488">
        <property type="term" value="P:phosphatidylinositol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051209">
        <property type="term" value="P:release of sequestered calcium ion into cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00275">
        <property type="entry name" value="C2_PLC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16213">
        <property type="entry name" value="EFh_PI-PLC21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13361">
        <property type="entry name" value="PH_PLC_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08591">
        <property type="entry name" value="PI-PLCc_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.190:FF:000084">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000024">
        <property type="entry name" value="1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.240:FF:000006">
        <property type="entry name" value="1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000008">
        <property type="entry name" value="1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1230.10">
        <property type="entry name" value="Phospholipase C beta, distal C-terminal domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="C2_dom"/>
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        <property type="entry name" value="C2_domain_sf"/>
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    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
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    <dbReference type="InterPro" id="IPR001192">
        <property type="entry name" value="PI-PLC_fam"/>
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    <dbReference type="InterPro" id="IPR016280">
        <property type="entry name" value="PLC-beta"/>
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    <dbReference type="InterPro" id="IPR042531">
        <property type="entry name" value="PLC-beta_C_sf"/>
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    <dbReference type="InterPro" id="IPR037862">
        <property type="entry name" value="PLC-beta_PH"/>
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    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
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    <dbReference type="InterPro" id="IPR053945">
        <property type="entry name" value="PLCB1-4-like_EFh"/>
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    <dbReference type="InterPro" id="IPR000909">
        <property type="entry name" value="PLipase_C_PInositol-sp_X_dom"/>
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    <dbReference type="InterPro" id="IPR001711">
        <property type="entry name" value="PLipase_C_Pinositol-sp_Y"/>
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    <dbReference type="PANTHER" id="PTHR10336:SF149">
        <property type="entry name" value="1-PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE PHOSPHODIESTERASE CLASSES I AND II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336">
        <property type="entry name" value="PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE C FAMILY PROTEIN"/>
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        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF17787">
        <property type="entry name" value="PH_14"/>
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    <dbReference type="Pfam" id="PF00388">
        <property type="entry name" value="PI-PLC-X"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF22631">
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        <property type="entry name" value="PLC-beta"/>
        <property type="match status" value="1"/>
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    <dbReference type="PRINTS" id="PR00390">
        <property type="entry name" value="PHPHLIPASEC"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="PH domain-like"/>
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    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS50007">
        <property type="entry name" value="PIPLC_X_DOMAIN"/>
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    <dbReference type="PROSITE" id="PS50008">
        <property type="entry name" value="PIPLC_Y_DOMAIN"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 6 9" id="KW-0378">Hydrolase</keyword>
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    <keyword evidence="5 6" id="KW-0807">Transducer</keyword>
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        <location>
            <begin position="700"/>
            <end position="816"/>
        </location>
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            <begin position="819"/>
            <end position="944"/>
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            <begin position="1"/>
            <end position="56"/>
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            <begin position="981"/>
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        </location>
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            <begin position="1064"/>
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            <begin position="1378"/>
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            <begin position="1133"/>
            <end position="1160"/>
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        <location>
            <begin position="1255"/>
            <end position="1282"/>
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            <begin position="9"/>
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            <begin position="29"/>
            <end position="47"/>
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            <end position="537"/>
        </location>
    </feature>
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        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
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        <location>
            <position position="420"/>
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    <sequence length="1423" mass="156053" checksum="65D250C6864AD913" modified="2020-08-12" version="1">MASLLTAADRQNSTTQSPQSLSGSCSPRNAASPNATAAAAATTAGGQPTAGGGGNTAVALNNPTAVKLGVMDVPKALQDGEKFIKWDEDSCNGTPVTLRVDVKGFFLYWVDQNHEMDMLDIATIRDVRTGQYAKKPRDMKLRQIVTMGSQDTLEEKTVTVCYGADFVNVNFINFCCTRKEIARLWCDELIRMAYNLTQLNGPAIMFLQKAYTKLCLQVDKSGKIPVKNIIKTFATNKDDRRRVEKALDVSGLPSGKGDTLALTKFQFEDFFNLYKNLSQRTEVEKVYDELVGTSKRRLMSTSQLVDFLNKTQRDPRLNEILHPYANTARARDLIQEYEPNKFNAQKGQLSFDGFLRYLMSEDNPIMAASKLDLSDDMDQPMAHYFINSSHNTYLTGHQLTGKSSVEIYRQSLLAGCRCVELDFWNGRTEEPVIVHGYTFVPEICAKDVLEAIAETAFKTSEFPVILSFENHCNPRQQAKIANYCREIFGDMLLDRPLDSHPLESNVELPPPALLKRKIIIKNKKKHHHHHHHHHKKAATVVSSPQQPPPPAGTTVVANNVSNSNSTAANAHATAVNNSSANSTNAASNTPGVTPAVNSNSTNAANPSLPPSQQDEPNGTVVVSNNNGTAAPLQATGNGEIPHHAPPLQQIRQSSKESTGSSDTDSSSDDESMPGTVVGPIGASEADKVPQTKETEAGAEISALVNYVQPVHFSSFENSEKKARFYEMSSFDEKQATTLLKERPIEFVNYNKHQLSRVYPAGTRFDSSNFMPQLFWNAGCQLVALNYQTLDLAMQLNLGIFEYNHRCGYILKPEFMRRKDRRLDPFAESTVDGIIAGTVMVTVISGQFLTDKKVGTYVEVDMFGLPADTVRKKFRTKIVRDNGINPVYDEEPFVFKKVVLPELASIRIAAYEEGGKLIGHRVLPVIGLCPGYRHLTLRTEVGQPIPLATLFLCIVVKDYVPDGLSDFAEALANPIKYQSEQEKRSKQLAVLQEDMEPSDEDSCTNKKGDTLRSSETNNTTSSPARQRSVAGTQSSQGGAGGTADSDAEAPLEAITKMVALPVAQSSSLDHSNVPAAKLSTASREPTTTGGGGGGSGAPIITTTTNTPNVATGEGLNAANGGKIVADPLEKIFEDKQIRKKREALEKELKALKKSHDKEKLKIHAAQKSTDLGGGDAPVKKSKFGMGNKLVKRFSSKNMADMNVKVPPCASVSDAEGTGDAQAERLRQICRDHAGSYREVLEKYHDIIYTLAEDLLRQSQDAQMKQLKTQLERETSEVMRQLQLSRKSEVKQLAMVHKDKDELERMKREVDSTLVEKGVTERVRLTATYERKRDELQRQHDSVKQGLEDHKLKARSMLEKEAESHAFISDTFLAHLNTSNSTTSCSSVGATTNHPPATSVSSGNICGAGGTAGSNHENNVNNAHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VZA6</accession>
    <name>A0A6E8VZA6_ANOCL</name>
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        <submittedName>
            <fullName evidence="6">PKS_ER domain-containing protein</fullName>
        </submittedName>
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    <organism evidence="6 7">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_040233722.1">
        <property type="nucleotide sequence ID" value="XM_040377788.2"/>
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    <dbReference type="RefSeq" id="XP_049464652.1">
        <property type="nucleotide sequence ID" value="XM_049608695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VZA6"/>
    <dbReference type="EnsemblMetazoa" id="ACON009224-RB">
        <property type="protein sequence ID" value="ACON009224-PB"/>
        <property type="gene ID" value="ACON009224"/>
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    <dbReference type="EnsemblMetazoa" id="ACON009224-RC">
        <property type="protein sequence ID" value="ACON009224-PC"/>
        <property type="gene ID" value="ACON009224"/>
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    <dbReference type="EnsemblMetazoa" id="ACON009224-RD">
        <property type="protein sequence ID" value="ACON009224-PD"/>
        <property type="gene ID" value="ACON009224"/>
    </dbReference>
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    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004784"/>
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    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030761"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Uncharacterized protein, isoform A"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ER"/>
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        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
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        <property type="entry name" value="Zn_alcohol_dh"/>
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    <dbReference type="PANTHER" id="PTHR43401">
        <property type="entry name" value="L-THREONINE 3-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR43401:SF2">
        <property type="entry name" value="L-THREONINE 3-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="5">
        <location>
            <begin position="19"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="83"/>
            <end position="104"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023002"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009224-PD"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="408" mass="44327" checksum="5722A053010BFC03" modified="2020-08-12" version="1">MSKLIRQVSIETPAPKLKDCVLNLVVPVPDTPAQGARILVVYAGACYRKNQSTSISSISSQLSDTGDITTSLHLLAKQMQSGSFTSISEEGPDSTPPQHHQTSVAIQHNPQAHQGVRDGALFPGFEVAGVIESLGSELKPDCGFKVGQRVILYPYEGVPHGYSELMVVPDLKYLIPIPDELPLAVAATLPTGALLAQSAIIAAHKIVDELLKSRPGEKVKILIVGTGGLALWAVRIAAQHFYTPATKDKIQITVASLRDEGFLVAKKCERVNIVQWNEDLYEKQLIERTHDACDGLVDIVIDFGTTSRSLHRSMQCLTKGGHIFISDEVAEKLLPKFSKRAEERGQTIEAAPTGTIEQLHQLVKLVSSNEIEPPPHSVFPSDQAADVVRKLASSEIPGRAILKFHDIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VZB0</accession>
    <name>A0A6E8VZB0_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="9">MFS_1_like domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. MFSD6 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VZB0"/>
    <dbReference type="EnsemblMetazoa" id="ACON010048-RC">
        <property type="protein sequence ID" value="ACON010048-PC"/>
        <property type="gene ID" value="ACON010048"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000020"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON010048"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037088"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd17335">
        <property type="entry name" value="MFS_MFSD6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024989">
        <property type="entry name" value="MFS_assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051717">
        <property type="entry name" value="MFS_MFSD6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16172">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 6-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16172:SF41">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 6-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12832">
        <property type="entry name" value="MFS_1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="22"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="329"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="370"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="403"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="444"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="474"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="507"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily associated" evidence="8">
        <location>
            <begin position="2"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="149"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="640"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="728"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="812"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="153"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="179"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="640"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="754"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="776"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="812"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="836"/>
            <end position="845"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010048-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="845" mass="93690" checksum="D2F06632127280F0" modified="2020-08-12" version="1">TSSLLPYLTIHMQSTGLTVEEIAIIYLALPFTTFLSPPITGFLVDKFGRYKPVVIMSLLLNALFHHSLLLIPQQEIPGKLPEAYVMRHPGTGNVEVWWSPCPSRECPEEEEIDIVVDQCLDHCLLQEQNPKIELAPPPTDLPLVVTPIGDEPNDPKDDLDLYVRNKGKGNKNKTADVLSSTSSSSSTTELYMDKSLLKETPEESEESEEGWVVAGSGDSAFIVLDMHPDLGEPIEQLGMEIEHDDNATVTDFKTRFGVALLWQQGVNVTALEEEDLRCGGLVMTSNMTLNSNIKLSEWAADCMVQRCRFRRNGPDICPPDYKESDDKIFWIYFLLRFLATTMLSAGVTIMDPIALTMIEKYGGDFGRERLFSSIGMAIFSPITGVLIDLFSKDLGYTDYSAAFYTYDILLIISSVTVFLMPLGEKLPADNVFKDLLNLLKLKHVIIFIWFLFLLGNFWGFIESFLFLYLKELGAPNYLLGITITVGTVSSIPFLYGAGRITKIVGHVNLIVIAFIAHACRLVGYSFIENAWWCFPFEAMEALSCHLMWVAAATYCALLAPKNLLATLIGVLGMAHFSLGRGSGSFFGGFLIGEVGTREAFRYMGLVAVAGGLAYKFIHSIWLRKYDNPDADDEAVEKGEAEKLYTDGDEPKTKDQGTSMSQERLSLMIKYNQIGSLTSLPRGSRGDVNDVFSKRRSSYNIEFVRAQKGGGSASKVDLLKSAVDINHKASHQHLRKEGKASDQSLNSKRADSAPRLNQSKNISQPILSAVVDEETEQRDSITEGEEENYKLELSKKASRGHVLNDIPEKTVEAVDTAAATPPVTPVVEGTDTTTTTTHHHHSEHSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VZD4</accession>
    <name>A0A6E8VZD4_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Gustatory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Gustatory receptor which mediates acceptance or avoidance behavior, depending on its substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the insect chemoreceptor superfamily. Gustatory receptor (GR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VZD4"/>
    <dbReference type="EnsemblMetazoa" id="ACON009256-RD">
        <property type="protein sequence ID" value="ACON009256-PD"/>
        <property type="gene ID" value="ACON009256"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007990"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON009256"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007635">
        <property type="term" value="P:chemosensory behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008049">
        <property type="term" value="P:male courtship behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050909">
        <property type="term" value="P:sensory perception of taste"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013604">
        <property type="entry name" value="7TM_chemorcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21143:SF133">
        <property type="entry name" value="GUSTATORY AND PHEROMONE RECEPTOR 32A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21143">
        <property type="entry name" value="INVERTEBRATE GUSTATORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08395">
        <property type="entry name" value="7tm_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="32"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="68"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="244"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="275"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="356"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009256-PD"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="382" mass="43479" checksum="034442821E2577DB" modified="2020-08-12" version="1">MASIELLGKILYVKSILVGVIPLRLPRSSWELLILCYCYTIAIAGPLLHFLLYLAWFVSVMVKFRVTFVVSICMHLGEVPFLIFPSCYLLSCRAKMKQLAELCTHVHSSSLLVGPFSLHAWYRKCCVFNLGYEAAHTVTLLANLALQCIFPEGMGKIYPQTLLWIALSNLTKSAVLLTMYGSVQYLMLCTVKLNELLPRLSSWTDPYRQETLRRLHFGRDGLMDFYGQLWQGAELANELFGPPLLAYLAIAFIHTTTIYYRVWSQLAGGLENQPLVITVHLIVQLVWVTMDLMFLMAVVGSCGQMRQESLKTQKLLLRLNLSPMDHKLKQSIEVFALQTLHQPIEFTACRMFTLDYTVLFSIAAAVTNYLIILIQFEMAIEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W0L3</accession>
    <name>A0A6E8W0L3_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="7">SERPIN domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the serpin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8W0L3"/>
    <dbReference type="EnsemblMetazoa" id="ACON009670-RA">
        <property type="protein sequence ID" value="ACON009670-PA"/>
        <property type="gene ID" value="ACON009670"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007514"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON009670"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033089"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045861">
        <property type="term" value="P:negative regulation of proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.39.10:FF:000035">
        <property type="entry name" value="Serine protease inhibitor (serpin) 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.39.10">
        <property type="entry name" value="Alpha-1-antitrypsin, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.497.10">
        <property type="entry name" value="Antithrombin, subunit I, domain 2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023796">
        <property type="entry name" value="Serpin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000215">
        <property type="entry name" value="Serpin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036186">
        <property type="entry name" value="Serpin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042178">
        <property type="entry name" value="Serpin_sf_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042185">
        <property type="entry name" value="Serpin_sf_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461:SF342">
        <property type="entry name" value="SERINE PROTEASE INHIBITOR 28DC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461">
        <property type="entry name" value="SERINE PROTEASE INHIBITOR, SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00079">
        <property type="entry name" value="Serpin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00093">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56574">
        <property type="entry name" value="Serpins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0722">Serine protease inhibitor</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026005870" evidence="5">
        <location>
            <begin position="26"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="domain" description="Serpin" evidence="6">
        <location>
            <begin position="64"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="31"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="31"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009670-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="547" mass="61643" checksum="60D87241B8BFA29D" modified="2020-08-12" version="1">MRSHYSAGWTLALVLLVSSLLAVSAANNRGQPAKPAAADQQANRLHAPTANTKKVSDSVTNLAAKIANALSNQKSKTEIFSPVSIAGALSLLLLGSGGQTQQELLAVMGLNTGQLSFQDIHLSFGRLFQDLVSNDPSLEPLVTWRLNDKCNRYNEDEEDEEDDFINSQSPSNEVDLLIQIGNGIFSQKGTKFDERYDKLAKDLYQSELKPLDFVGDETGSVRYINSWVHNQTHGRIADIVSHISADTILMIVNTLYFRGLWEEAFQPMATRNRRFFPNGPDGPDSFDIPMMAKSHCMPYYFWQEENVRVLGVPYRQNVTMYIFMPMNSTRQLVQSMQSKITAERVNDIVTKMKMKSVTVMFPKMHISNSISLKNVLQQLGVYTLFERNEADLKRLLASNTTDRFGGDPLDLLEVLQDTKENAILLLQQQIPDCIIEERSNIDRGECLKDHCAYGGKTCVCCIESFRRRRRRDAFTPSKNDPIFVNEMLHKVDLTVNERGTEGGAATATLIDRISSQINFIVNGPFMMVIREETTRLPLFYGNIYNPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W0M0</accession>
    <name>A0A6E8W0M0_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Kinesin-like protein unc-104</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Required for presynaptic maturation, has a role in axonal transport of dense-core vesicles carrying synaptic vesicle precursors, components required for the morphological transformation of axonal growth cones to mature boutons.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON010519-RB">
        <property type="protein sequence ID" value="ACON010519-PB"/>
        <property type="gene ID" value="ACON010519"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON010519-RE">
        <property type="protein sequence ID" value="ACON010519-PE"/>
        <property type="gene ID" value="ACON010519"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001094"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON010519"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037311"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904115">
        <property type="term" value="C:axon cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048490">
        <property type="term" value="P:anterograde synaptic vesicle transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021700">
        <property type="term" value="P:developmental maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051222">
        <property type="term" value="P:positive regulation of protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040012">
        <property type="term" value="P:regulation of locomotion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051960">
        <property type="term" value="P:regulation of nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010975">
        <property type="term" value="P:regulation of neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008582">
        <property type="term" value="P:regulation of synaptic assembly at neuromuscular junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd22705">
        <property type="entry name" value="FHA_KIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01365">
        <property type="entry name" value="KISc_KIF1A_KIF1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01233">
        <property type="entry name" value="PH_KIFIA_KIFIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.20:FF:000001">
        <property type="entry name" value="Kinesin family member 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000004">
        <property type="entry name" value="Kinesin-like protein isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000204">
        <property type="entry name" value="kinesin-like protein unc-104 isoform X6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2520">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022164">
        <property type="entry name" value="Kinesin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022140">
        <property type="entry name" value="Kinesin-like_KIF1-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032405">
        <property type="entry name" value="Kinesin_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049780">
        <property type="entry name" value="PH_KIFIA_KIFIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47117:SF10">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIF1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47117">
        <property type="entry name" value="STAR-RELATED LIPID TRANSFER PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12473">
        <property type="entry name" value="DUF3694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12423">
        <property type="entry name" value="KIF1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16183">
        <property type="entry name" value="Kinesin_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="9 12" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="16">
        <location>
            <begin position="3"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="15">
        <location>
            <begin position="1556"/>
            <end position="1654"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="398"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="966"/>
            <end position="998"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="359"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="631"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="398"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="412"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="982"/>
            <end position="997"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="97"/>
            <end position="104"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00056070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010519-PE"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1706" mass="191510" checksum="036548777B22467E" modified="2020-08-12" version="1">MSSVKVAVRVRPFNSREIARESKCIIEMAGNTTCITNPKVPPGSSESVKRFNYDYSYWSHDPRDLEFSTQAMVYSDIGEEMLQHSFDGYNVCIFAYGQTGAGKSYTMMGKQEDGQEGVIPMICKDLFRRIQETESDDLKYSVEVSYMEIYCERVRDLLNPKNKGNLKVREHPLLGPYVEDLSKLAVTSYQDIHDLIDEGNKARTVAATNMNETSSRSHAVFTIFFTQKRQDRMTSLETEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEIASKNKKSKKADFIPYRDSVLTWLLRENLGGNSKTAMIAAISPADINYDETLSTLRYADRAKQIVCKAVVNEDANAKLIRELKEEIQKLRELLKAEGIEVQEGPDGKVVCEKRDANSEDGEKKIHSPNRNRKRTGSSTEMAVDQLQASEKLIAELNETWEEKLKRTEQIRVQREAVFAEMGVAVKEDGITVGVFSPKKSPHLVNLNEDPTLSECLLYYIKDGLTRLGTSEANVPQDIQLSGSHILKEHCVFENKDGVVTLVPHKDALVYVNGRKVIEPEVLQTGSRVILGRNHVFRFTHPEQAREKREKNKEVDVCETPGNSGEIADWNFAQCELLEKQGIDLKAEMEKRLLALEEQYKREKRAADQEFEEQRKTYEARIDALQKQVEEQSMTMSMYSSYSPEDFHQEEDIFVNPLFESCWTAREAGLAAWAFRKWRYHQFTSLRDDLWGNAIFLKEANAISVELKKKVQFQFTLLTDTLYSPLPPELTPAPAVGALTNGGQEDEFGQNPIPRTTVAVEVTDTKNGATHYWSLEKLRQRLELMREMYHNEAELSPTSPDYNVESLTGGDPFYDRFPWFRMVGRSFVYLSNLLYPVPLVHKVAIVNERGDVRGYLRVAVQPVMDEENADFSNGVKQSARILFDEEQNGQHKVPKIRTIPDKDEKYIEGGNEMGTKLEELEQEDADSGRGDSSVASELHESNDQEPGEHLQPGKEFTFRVTVLQATGIAAEYADIFCQFNFLHRHEEAFSTEPVKNSGSGAPLGFYHVQNITVPVTKSFIEYLKTQPIVFKVFGHYQHHPLHKDAKQDCQITRPPPRRMLPPSIPISQPVRSPKFGPLPCPPSSTVLAKHDVLVWFEICELAPNGEYVPAVVDHSDDLPCRGLFLLHQGIQRRIRITIVHEPTPEVKWKDIRELVVGRIRNQPEPADDEDSDSCVLSLGLFPGEVLEVPGDDRSFFRFEAAWDSSLHNSALLNRVTQTGEQIYITLSAYLELDNCARPAIITKDLSMIIYGRDARTGPRSLKHLFSGQYRNPEANRLSGVYELSLRRASEAGSPGVQRRQRRVLDTSSTYVRGEENLHGWRPRGDSLIFDHQWELEKLTRLEEVGRVRHLLLLRERLGMDTTPNPTTKTEKDVCNLAQRASASPVHMVIPPSPQTPVKDQQAPAMPERELSVRETELVWKCVKLIQGRIGCKELGAEGNGSGTQASDASPGDEGCADMNASYISSNSIELCSPERVDMPNGWEAPAPAPQTQDLSLRLYVPELEEIRVSPVVARKGYLNVLEHGGSGWKKRWVTVRRPYVFIFRSDKDPVERAVLNLATAQVECSEDQAAMVKVPNTFSVVTKHRGYLLQTLGDKEVHDWLYAINPLLAGQIRSRLARKNISSISSTGSGAGDQQQATTGASALAVAAAQGNGGGSSSGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W2P3</accession>
    <name>A0A6E8W2P3_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DH domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8W2P3"/>
    <dbReference type="EnsemblMetazoa" id="ACON010473-RB">
        <property type="protein sequence ID" value="ACON010473-PB"/>
        <property type="gene ID" value="ACON010473"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004053"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON010473"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037586"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030139">
        <property type="term" value="C:endocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043542">
        <property type="term" value="P:endothelial cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007266">
        <property type="term" value="P:Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040181">
        <property type="entry name" value="PKHG5/7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13217:SF11">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY DOMAIN-CONTAINING FAMILY G MEMBER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13217">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY DOMAIN-CONTAINING FAMILY G MEMBER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DH" evidence="2">
        <location>
            <begin position="901"/>
            <end position="1093"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="269"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="326"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="520"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="786"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1206"/>
            <end position="1226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="269"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="283"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="330"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="791"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="816"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1209"/>
            <end position="1226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010473-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1278" mass="139221" checksum="8F7F5E30AD7B2E71" modified="2020-08-12" version="1">MDENVQRSNESIKQRNLVHSIEQISPIRDVYSILPESNVHSTGQSQTLHTQPSASSSPTHISNTSPSAATSLSTLLSPTKISQDVNLQSTSKNGAHQETFSNSLATATPAGFSLILPQNLNRSFDQTAAQSPPKYQIYHQFSRISSTTQQFIQRQQQQQLQQQEQHQQIFHPQAQPLQLQQSPQTQPASPVTNYQTPLFRKASYGSSHYAHYSNVDPLTVNAGTLSARPNNTYHQYQTTHQCSKTVDQRSNNPFILNFNNISGCCLKNSPTSSSSPPATSPTDRSITSAPITGNPQSVTSGSSSSAITAVVSSGTKRTRSLLLKSTSPLQQTTHSSPSHTVSSPQGPPVPPRLSHIHAIGSVSPANTGPTFSNHPSIDPISTTTSSNASSEAIVMSSIVTGTCYVTPPTPAPLITSDKHRELPSMTFGSVTPTVVSNTLPDSTTLTASGHNKTPTDGNESGLLTKEMIDKSVRKKRDDFLRTTMKICLVVSPPSKLQMKSLSLTHLDEIERQATTPTIITNDPHVRKSDNSDTSNTSIASASGSAGYGGNTVSGGSSTHAVSPKDVCLTVTSGSALIDISNSDGGCINPAYDNGEGSHRRQAGNSFTVSEVDARIVQKQNSTEKVHECQSSGTCCMLSSPSCVKAALGYSTKLGGTSSTSSLKKKSSSFLHRERKKPVLTRSQVSSSEYFSVCFESDQNDPGVLIPATKGVRLAECLRASLSRRSLSFSQIYVTDNGFNNPHETGLRYTEPLDANMDISALAGRTLIVSERDGAPNRRTGGHLTLQKAASVGNQPSIMSSNSKLFTSTSTDDNYDQSPKASSASGKQSKQKWTIPFGSKGPQKSKLCELLDSYNKEIPKSTSSSSNFNNPEYVDALVGLRNLPQCWTDIVNCAGMSEAETKIQSAIWELVTTEVYYILALQTVTDLFLACLEDIQSQNILTDVDQNKLFSNIRDIWEANLRFWTLYLHPMVKHSQRTKDPMSIYYFQRGFVDFATIFTPYTKYCAEQSTCQFYCKEMYHNNALFMTYCAWCESQKMCNRLRLADILVRPMQRLTKYGLLLAAIKKHINDEAEGEAIDAMIHSVEEFVAGVNSHLTTRQESERLKGINARIDCYEVVDSNNETLDRLVKQHSIMFDLCQPMRGIDHGRKVFVEGDLKYKDGTGKATLRRGFAFSTSIKNPPLIKTRNVSSQQSFALSQQQSFNSASLAQHQQCLPSPSNTLHGAPNNSGTNSAIEGCAVASGLTLSSANVSFFKRKNSFQSVPNNSATSSVDQSLPNET</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W2P5</accession>
    <name>A0A6E8W2P5_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Alpha-endosulfine</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Protein phosphatase inhibitor that specifically inhibits protein phosphatase 2A (PP2A) during mitosis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the endosulfine family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8W2P5"/>
    <dbReference type="EnsemblMetazoa" id="ACON010476-RA">
        <property type="protein sequence ID" value="ACON010476-PA"/>
        <property type="gene ID" value="ACON010476"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_003096"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON010476"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037262"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004864">
        <property type="term" value="F:protein phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006760">
        <property type="entry name" value="Endosulphine"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10358">
        <property type="entry name" value="ENDOSULFINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10358:SF6">
        <property type="entry name" value="ENDOSULFINE, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04667">
        <property type="entry name" value="Endosulfine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4" id="KW-0498">Mitosis</keyword>
    <keyword evidence="3 4" id="KW-0650">Protein phosphatase inhibitor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="16"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="32"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="49"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010476-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="126" mass="13800" checksum="4598CDEDFF9A4B4C" modified="2020-08-12" version="1">MSAEESTEQQTAIAEVAEDQQSEDEQQQSVSELEKQEEEKMKAKYGSNVGMGGPRGLGGHSAFLQKRLQKGQKYFDSGDYQMAKQKGGGVKQVFANKVPTGEAIPTPETVPVRKTSIIQTCNKFQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W2S7</accession>
    <name>A0A6E8W2S7_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8W2S7"/>
    <dbReference type="EnsemblMetazoa" id="ACON010502-RA">
        <property type="protein sequence ID" value="ACON010502-PA"/>
        <property type="gene ID" value="ACON010502"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACON010502"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010502-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="100" mass="11685" checksum="C923CA22B9E4744B" modified="2020-08-12" version="1">MRRKCQTSPVRAIFPGHPECCTIGYHVRLHLKFPDKINSLHRRRHRPRCLRWFDSFTHGNVHAFSNASSYVLSNTHFSTLPFTPPSSYRDPVETLGHRSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W324</accession>
    <name>A0A6E8W324_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="10">FIP-RBD domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cleavage furrow</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Midbody</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Recycling endosome membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8W324"/>
    <dbReference type="EnsemblMetazoa" id="ACON010445-RB">
        <property type="protein sequence ID" value="ACON010445-PB"/>
        <property type="gene ID" value="ACON010445"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002532"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON010445"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037687"/>
    <dbReference type="Proteomes" id="UP001105220">
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030139">
        <property type="term" value="C:endocytic vesicle"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:recycling endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:regulation of cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Nuclear fallout, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FIP-RBD_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019018">
        <property type="entry name" value="Rab-bd_FIP-RBD"/>
    </dbReference>
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        <property type="entry name" value="Rab11-FIP3/4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051977">
        <property type="entry name" value="Rab11-interacting_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15726:SF7">
        <property type="entry name" value="NUCLEAR FALLOUT, ISOFORM J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15726">
        <property type="entry name" value="RAB11-FAMILY INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25450">
        <property type="entry name" value="Rab11-FIP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09457">
        <property type="entry name" value="RBD-FIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144270">
        <property type="entry name" value="Eferin C-derminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51511">
        <property type="entry name" value="FIP_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0967">Endosome</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="FIP-RBD" evidence="9">
        <location>
            <begin position="283"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="63"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010445-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="347" mass="40494" checksum="ABE30D0076A1C717" modified="2020-08-12" version="1">MRQNLNVPHYLTENRLNATVTTTYQSQKLVVHLYRSSSFNSSGRSSNCDTAEDMYSDVSLEDVQDINHKLEILQRQVTNLADTQSNVDDRTSRTKTEYAVLQARYHMLEEQLRETELRAEERLAEEQKRHRELLARVEREAKLQNENCQIRIRTMEMEVTSLREEIQRLRLQSDKQAADLHATEEKLEKARDSLMISQQDLAEARAEEKKHRAEKQAAEELMVELGKECERLRSERGPALPTTSPESLRLEELHQEMDELRQKNKSLEEANEELQAMMLTRSIEEGRNLLNGTSNSLAQELEAMSQNQLQVAFQEKEEENRRLKHYIDTMLLNVVENYPQLLEVKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W347</accession>
    <name>A0A6E8W347_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Acyl carrier protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Carrier of the growing fatty acid chain in fatty acid biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the acyl carrier protein (ACP) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040238735.1">
        <property type="nucleotide sequence ID" value="XM_040382801.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8W347"/>
    <dbReference type="EnsemblMetazoa" id="ACON010464-RB">
        <property type="protein sequence ID" value="ACON010464-PB"/>
        <property type="gene ID" value="ACON010464"/>
    </dbReference>
    <dbReference type="GeneID" id="120959426"/>
    <dbReference type="CTD" id="38154"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010474"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON010464"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033023"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000035">
        <property type="term" value="F:acyl binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000036">
        <property type="term" value="F:acyl carrier activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1200.10:FF:000003">
        <property type="entry name" value="Acyl carrier protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01217">
        <property type="entry name" value="Acyl_carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003231">
        <property type="entry name" value="ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00517">
        <property type="entry name" value="acyl_carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002148">
        <property type="entry name" value="PRK00982.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20863">
        <property type="entry name" value="ACYL CARRIER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20863:SF28">
        <property type="entry name" value="ACYL CARRIER PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="13 14" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="6 14" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 14" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Carrier" evidence="15">
        <location>
            <begin position="72"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010464-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="151" mass="17010" checksum="3F72A2B1AA1D391F" modified="2020-08-12" version="1">MASLVNSVRGLACRNTSLLRCVFLRSVSSATVARRDRCNEKFLTAPASALLSPFEPKVWSVHRFFATKPKVDEIKQRVLKVVGAYDKVTADKLTLESHFINDLGLDSLDHVEVIMAMEDEFGFEIPDGDAEKLFRPADIVQYIADKEDIYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W6X0</accession>
    <name>A0A6E8W6X0_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the prominin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8W6X0"/>
    <dbReference type="EnsemblMetazoa" id="ACON011954-RB">
        <property type="protein sequence ID" value="ACON011954-PB"/>
        <property type="gene ID" value="ACON011954"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007850"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON011954"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032193"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008795">
        <property type="entry name" value="Prominin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11238">
        <property type="entry name" value="PROMININ ISOFORM D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11238:SF9">
        <property type="entry name" value="PROMININ, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05478">
        <property type="entry name" value="Prominin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="85"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="145"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="425"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="461"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="786"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="827"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="832"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="842"/>
            <end position="851"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON011954-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="861" mass="96625" checksum="2CAF5E34F8FC1D4D" modified="2020-08-12" version="1">MQAMEQSVAIIPHDYPEPVATDSYIIPTLGLYHGSFVFTYLSTFLSYITPYDLPVVYCILELLRDAFHTKVSFLQLILEALKVESGYICLIAIFFLISLIPLCYTIAWGCAKEPDDDLNPGPTVDAIIQAEELSLEESLHCRKSFLGLMLQVVILLLIAGVIAMFVTNEQIASAVDHTPTVVRSSLLDAATYLRDAESQMRFVIAQGLATATERIRDDLEAVDKLLGEPIQHHLALFTGIDIIFESIIEISTSSSNLSRRIHLLQDALARAAKISYEAEYRLDELQIQLTVLQRQCTFRDRPLCDTLRIRNFEDTGLIDRIRKLQADRTLAKLMAMGEASRNSSMHALSQELTLARATFRNYAGEVRNATEDQADLVEERLEELSNDTEATVSVLSQTIGELVGQVNRTANFSDSFFDRYREIGAILWLAGLATTIMTLLVTLILLSALSCGCCHADNKAGITLILGAVCICIASLALSGFTMMEMLLGAHGQLFICHPLYNEPDYTVLQKLIDKPGLIYPTEPQYGIIGELLRQAAPADARWSQPVQISLSKALNECEQGRGSYGTFQLDSLLNLSAKIEHRKRPELERAIESVGASEEPFLGFTVRIQGILEDMLYDSDLNMTSTRIELTQLSPDKDVLTFIDQLQRVSAQIQDVATSSRMTTLGSRAKRLQLSLLAPLEQLRGDIVYHLTALELQLSPWAAQVNKSLTHLRNAQSFLDTDAAEVCYNRSDTYRARLRGHLDAYRNYTTMVLNDRCASCRPLFDIFDAMRMLFCHHIMDPMNGLWFSAFLCLFLWAVATPLSLMLSSTYRRLEILSTKLQHSNSHRARASVREDQRESSHWSTQRSTVSVMPDIDRSNW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W6X3</accession>
    <name>A0A6E8W6X3_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="7">RRM domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8W6X3"/>
    <dbReference type="EnsemblMetazoa" id="ACON013145-RC">
        <property type="protein sequence ID" value="ACON013145-PC"/>
        <property type="gene ID" value="ACON013145"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010950"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON013145"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031655"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12684">
        <property type="entry name" value="RRM_cpo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000037">
        <property type="entry name" value="RNA-binding protein with multiple splicing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034788">
        <property type="entry name" value="Cpo_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10501">
        <property type="entry name" value="U1 SMALL NUCLEAR RIBONUCLEOPROTEIN A/U2 SMALL NUCLEAR RIBONUCLEOPROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="80"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="24"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="32"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON013145-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="243" mass="25747" checksum="64C3CBCD5A33B249" modified="2020-08-12" version="1">MFSSPVVAQSNTVVSSPSMNGALNVNAANNNHTGLGQTGQSATGNLKMDSSMGQMTPMAPLALSQSMDSVNTASNEEEVRTLFVSGLPMDAKPRELYLLFRAYEGYEGSLLKVTSKNGKTASPVGFVTFNTRAGAEAAKQDLQQGVRFDPDMPQTIRLEFAKSNTKVSKPKPQPNTAATAAHPALMHPLTAGRTPFFPGGPELWHHPLAFSAAAAELPSAAALQHATLVHPALHPQVPVRSYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WAL6</accession>
    <name>A0A6E8WAL6_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Phosphotransferase</fullName>
            <ecNumber evidence="14">2.7.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes the phosphorylation of various hexoses to hexose 6-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>D-fructose + ATP = D-fructose 6-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16125"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37721"/>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:16126"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>D-glucose + ATP = D-glucose 6-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17825"/>
            <dbReference type="ChEBI" id="CHEBI:4167"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61548"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:17826"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>D-mannose + ATP = D-mannose 6-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11028"/>
            <dbReference type="ChEBI" id="CHEBI:4208"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58735"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:11029"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a D-hexose + ATP = a D-hexose 6-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22740"/>
            <dbReference type="ChEBI" id="CHEBI:4194"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:229467"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:22741"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate metabolism; hexose metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the hexokinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.-" evidence="14"/>
    <dbReference type="RefSeq" id="XP_040239948.1">
        <property type="nucleotide sequence ID" value="XM_040384014.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_049466236.1">
        <property type="nucleotide sequence ID" value="XM_049610279.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_049466237.1">
        <property type="nucleotide sequence ID" value="XM_049610280.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_049466238.1">
        <property type="nucleotide sequence ID" value="XM_049610281.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8WAL6"/>
    <dbReference type="EnsemblMetazoa" id="ACOM038162-RA">
        <property type="protein sequence ID" value="ACOM038162-PA"/>
        <property type="gene ID" value="ACOM038162"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON029633-RC">
        <property type="protein sequence ID" value="ACON029633-PC"/>
        <property type="gene ID" value="ACON029633"/>
    </dbReference>
    <dbReference type="GeneID" id="120960075"/>
    <dbReference type="CTD" id="45875"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002473"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON029633"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031770"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00180"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00242"/>
    <dbReference type="Proteomes" id="UP000075882">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005536">
        <property type="term" value="F:D-glucose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008865">
        <property type="term" value="F:fructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004340">
        <property type="term" value="F:glucokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001678">
        <property type="term" value="P:intracellular glucose homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd24019">
        <property type="entry name" value="ASKHA_NBD_HK_meta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000095">
        <property type="entry name" value="Phosphotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.367.20:FF:000005">
        <property type="entry name" value="Phosphotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.367.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001312">
        <property type="entry name" value="Hexokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019807">
        <property type="entry name" value="Hexokinase_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022673">
        <property type="entry name" value="Hexokinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022672">
        <property type="entry name" value="Hexokinase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19443">
        <property type="entry name" value="HEXOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19443:SF16">
        <property type="entry name" value="HEXOKINASE TYPE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00349">
        <property type="entry name" value="Hexokinase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03727">
        <property type="entry name" value="Hexokinase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00475">
        <property type="entry name" value="HEXOKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00378">
        <property type="entry name" value="HEXOKINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51748">
        <property type="entry name" value="HEXOKINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 14" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="6 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Hexokinase N-terminal" evidence="15">
        <location>
            <begin position="11"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="Hexokinase C-terminal" evidence="16">
        <location>
            <begin position="212"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00050361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00059457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU362007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF03727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON029633-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="455" mass="50733" checksum="6A01EF42C7B32D92" modified="2020-08-12" version="1">MGVIKDSFTKIREQCKELILTDKQIEEIMRRVIKEINRGLSKETHAEADVKCFITYVQDLPNGKEKGKFLALDLGGTNFRVLLIHLKDENDFEMLSKIYAIPQSIMLGSGTQLFDHIAECLANFMKEHSVYEERLPLGFTFSFPLTQLGLTKGILARWTKGFNCSGVVGEDVVQLLKDAIARRGDVQIDICAILNDTTGTLMSCAWKNHNCRIGLIVGTGSNACYVERVENCDLFDGPKSGPNIKQHVMINTEWGAFGDDGALDFVRTEYDREIDQHSINPGRQLQEKMISGMYMGELARLAIVRFTKAGLLFGGVGSDILFKRGQFFTKYVSEIESDKPGTYYNCREVLEELGLEHATDEDCANVRYICECVSSRAAHLVSAGIAALINKMDEPSVTVGVDGSVYRFHPKFHDLMVQKIRQFVKPHISFDLMLSEDGSGRGAALVAAVACREAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WAM9</accession>
    <name>A0A6E8WAM9_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="12">ABC transporter domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040220724.2">
        <property type="nucleotide sequence ID" value="XM_040364790.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8WAM9"/>
    <dbReference type="EnsemblMetazoa" id="ACON029451-RA">
        <property type="protein sequence ID" value="ACON029451-PA"/>
        <property type="gene ID" value="ACON029451"/>
    </dbReference>
    <dbReference type="GeneID" id="120948452"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010208"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON029451"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_029700"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03213">
        <property type="entry name" value="ABCG_EPDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000891">
        <property type="entry name" value="ATP-binding cassette sub-family G member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050352">
        <property type="entry name" value="ABCG_transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041:SF78">
        <property type="entry name" value="ABC TRANSPORTER EXPRESSED IN TRACHEA, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="634"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="663"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="705"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="743"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="772"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="804"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="853"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="166"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="443"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="488"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="443"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="519"/>
            <end position="537"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON029451-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="883" mass="95853" checksum="BF2A87FF49525804" modified="2020-08-12" version="1">MDMEVAVDAMEHPHSGTPADGSVRELPALICVRPTSPGGGSAFRLGALPPSGCSSLEDLDDTVEGTTIYQLSCDNHYNLNFYANRCDSGSDAGSMKNFKNHPTTVWVKRNSQSALCNGAIMTNGQTKDSNLAHGLVRKVLNNSDGNGQKKPMVSLTHLPKRPPIDIQFMDMSYSVSEGHKRGYKTILKGINGKFRSGELTAIMGPSGAGKSTLMNILAGYKTSHLSGSVLINGKERNLRKFRKLSCYIMQDDRLLPYLTVREAMMVSANLKLGKDISVSAKRAVVEEIIETLGLLDAASTLTLNLSGGQRKRLSIALELVNNPPVMFFDEPTSGLDSSTCSQLIALLKSLARGGRTIVCTIHQPSARIFELFDNLYVLAEGQCIYQGRVNGLVPFLASLGLECPSYHNPANYVMEVACGEHGDWNSKLVTAVNNGKCNNYNQPLAQSKSTSNKNVSLDGTAPGATEREDELALLKTVVSTGDYTAISMPPAGNAAAPEAQLNNESKQPAGGTDRKPMLGNGAEAASAASQSTTTTTTTATTAVGATGASVNPSSDATGPLLPAVVVPPAGPAQTQPGCKATCTTSLLDSTESVSITVPKKQAGFPTSGWMQFWILLKRTMITIMRDQTLTQMRLLSHVIVGAIIGMIYYDIGNDASKIMSNAGCIFFTTMFTMFTAMMPTILTFPTEMAVFVREHLNYWYSLKSFYFAKTIADLPFQVLFTSVYVIVVYYLTSQPMDPKRVGMFVLICILTSLVAQSLGLLIGAGMSVETGVFLGPVSTIPIILFSGFFVNFDVIPSYLQWVTYVSYVRYGFEGAMVSVYGIEREKLACTEIYCHFRSPKKFLEEMSMDNAEYWIDATALFGFFIALRVIAYFVLRWKLHSIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WAP2</accession>
    <name>A0A6E8WAP2_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phosphoinositide phospholipase C</fullName>
            <ecNumber evidence="3">3.1.4.11</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + H2O = 1D-myo-inositol 1,4,5-trisphosphate + a 1,2-diacyl-sn-glycerol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:33179"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
            <dbReference type="ChEBI" id="CHEBI:203600"/>
            <dbReference type="EC" id="3.1.4.11"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.1.4.11" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8WAP2"/>
    <dbReference type="EnsemblMetazoa" id="ACON029661-RA">
        <property type="protein sequence ID" value="ACON029661-PA"/>
        <property type="gene ID" value="ACON029661"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007025"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON029661"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042366"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032587">
        <property type="term" value="C:ruffle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004435">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate phospholipase C activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046488">
        <property type="term" value="P:phosphatidylinositol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010634">
        <property type="term" value="P:positive regulation of epithelial cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051209">
        <property type="term" value="P:release of sequestered calcium ion into cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00275">
        <property type="entry name" value="C2_PLC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11825">
        <property type="entry name" value="SH3_PLCgamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000119">
        <property type="entry name" value="1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000199">
        <property type="entry name" value="1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.190:FF:000043">
        <property type="entry name" value="1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001192">
        <property type="entry name" value="PI-PLC_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001711">
        <property type="entry name" value="PLipase_C_Pinositol-sp_Y"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336:SF159">
        <property type="entry name" value="1-PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE PHOSPHODIESTERASE GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336">
        <property type="entry name" value="PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE C FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00387">
        <property type="entry name" value="PI-PLC-Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00390">
        <property type="entry name" value="PHPHLIPASEC"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00149">
        <property type="entry name" value="PLCYc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50008">
        <property type="entry name" value="PIPLC_Y_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="5">
        <location>
            <begin position="84"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="PI-PLC Y-box" evidence="7">
        <location>
            <begin position="245"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="6">
        <location>
            <begin position="361"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="218"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON029661-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="507" mass="58005" checksum="33BBBB35B502D186" modified="2020-08-12" version="1">AHGKFKHCRIRVEGRLYEMGGMEFESLVDLVNFYSKHPLYRKVTLTYPIPREMIRRINTMDVSVGPPVLFPGGYGYMDPATVTNENVTVKALYDYKAQRDDELSFCKHAIITNVVKRGNDWWLGDYGGKRQHYFPANYVQELSAMDDGTLVEEGANEPLMLGSLQKGSLDVHGAVVEMAYSNHPDIERILRIQNPTMQNVFEVGVQTKELAYEWMCAIKEAAQNASAMENERRKMERNSRVAKEMSDLIIYCRSVPFKHHPTAWVFYEMSSFPETKAEKYFLQAETRALFVRYHRNHLSRVYPKGQRLDSSNYHPTALWNCGSQMIALNFQTPDKPMQLNQAKFRDNGGCGYLLKPEFMFRDEFDPTDPNTLVGVGEVIVNVRIIGGRNLCKVSRNITSPLVEVEVLGASFDGGIKHRTRAIADNGLNPIWNEICEFRIANPHFAMIRFEVQDEDMFGEPNFIGQAVFPLGSIRTGYRSVVLRNKYSEELELATLLVHTSIRPLQTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WB13</accession>
    <name>A0A6E8WB13_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">May be involved in the metabolism of insect hormones and in the breakdown of synthetic insecticides.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 14">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Endoplasmic reticulum membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Microsome membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 15">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8WB13"/>
    <dbReference type="EnsemblMetazoa" id="ACON013511-RB">
        <property type="protein sequence ID" value="ACON013511-PB"/>
        <property type="gene ID" value="ACON013511"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009977"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON013511"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037074"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11056">
        <property type="entry name" value="CYP6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050476">
        <property type="entry name" value="Insect_CytP450_Detox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24292">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24292:SF84">
        <property type="entry name" value="CYTOCHROME P450 28A5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 14 15" id="KW-0349">Heme</keyword>
    <keyword evidence="11 14 15" id="KW-0408">Iron</keyword>
    <keyword evidence="13 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 14 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0492">Microsome</keyword>
    <keyword evidence="12 15" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="10 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="134"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="14">
        <location>
            <position position="343"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON013511-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="19">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="399" mass="45558" checksum="52B3505B7F613825" modified="2020-08-12" version="1">MLLILFGTVVAFVALVYIFLTWEFGFWEKYGVKGPKPKILLGNIPYLLTKKRHTVYNYDQLYIKAMAHLMDEVCEHMTDYLRTQAKSSDGVATLDAKEVMAKYTTDVVASCIFAIDAQSFVKENPEIRLMGKRIMNFNFVVQLALLVTTFFPSDAIRYRKENKINRTDFLDHLLQLQDRKQITDIDIAGHGVSFFADGFETSSIVLTYCLYDLAKYPTIQQELRDEIAKAKQAGGGTMLYEQLGEMALLEQIICESMRIHPLMAVMAKRCTADTTLVGPKDRKIFVKKGTTVVLPYYSICFDAEHFADPYKYDPKRFAAENGGSKAYRERGVYFPFGDGPRMCLGMRFAVTQVRRAIVEVVERFSISVSSKTIEPFEMDPDQFMLMPKGSIWLDFKPIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WCT2</accession>
    <name>A0A6E8WCT2_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Microtubule-actin cross-linking factor 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON029051-RH">
        <property type="protein sequence ID" value="ACON029051-PH"/>
        <property type="gene ID" value="ACON029051"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007811"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON029051"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040800"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030056">
        <property type="term" value="C:hemidesmosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005882">
        <property type="term" value="C:intermediate filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045104">
        <property type="term" value="P:intermediate filament cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042060">
        <property type="term" value="P:wound healing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21189">
        <property type="entry name" value="CH_PLEC-like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00176">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000260">
        <property type="entry name" value="Kakapo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000275">
        <property type="entry name" value="Kakapo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000001">
        <property type="entry name" value="Microtubule-actin cross-linking factor 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1290.10:FF:000014">
        <property type="entry name" value="microtubule-actin cross-linking factor 1 isoform X21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000110">
        <property type="entry name" value="Short stop, isoform AA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000031">
        <property type="entry name" value="Short stop, isoform J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000022">
        <property type="entry name" value="Short stop, isoform K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000030">
        <property type="entry name" value="Short stop, isoform K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000044">
        <property type="entry name" value="Short stop, isoform K"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Short stop, isoform K"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Short stop, isoform N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000047">
        <property type="entry name" value="Short stop, isoform N"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Short stop, isoform N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="29"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.920.20">
        <property type="entry name" value="Gas2-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1290.10">
        <property type="entry name" value="Plakin repeat"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041615">
        <property type="entry name" value="Desmoplakin_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003108">
        <property type="entry name" value="GAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036534">
        <property type="entry name" value="GAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049538">
        <property type="entry name" value="PCN-like_spectrin-like_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043197">
        <property type="entry name" value="Plakin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035915">
        <property type="entry name" value="Plakin_repeat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001101">
        <property type="entry name" value="Plectin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018159">
        <property type="entry name" value="Spectrin/alpha-actinin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002017">
        <property type="entry name" value="Spectrin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23169">
        <property type="entry name" value="ENVOPLAKIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23169:SF23">
        <property type="entry name" value="SHORT STOP, ISOFORM H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02187">
        <property type="entry name" value="GAS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17902">
        <property type="entry name" value="SH3_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00435">
        <property type="entry name" value="Spectrin"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21019">
        <property type="entry name" value="Spectrin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21020">
        <property type="entry name" value="Spectrin_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00243">
        <property type="entry name" value="GAS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00250">
        <property type="entry name" value="PLEC"/>
        <property type="match status" value="31"/>
    </dbReference>
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        <property type="entry name" value="SPEC"/>
        <property type="match status" value="35"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143575">
        <property type="entry name" value="GAS2 domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
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                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
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                <person name="Galvin B.D."/>
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    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00074533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON029502-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="916" mass="102088" checksum="63209CB2502E95AA" modified="2020-08-12" version="1">MMSQHLNSMLEHLKMGSLMSKNSSSGKQPQQQQQPSQQQQQPPQQVVPSSSANGTIPAGANGAPNGSCANNNVHCNHHSSPGATHHNNNNNTSSVNHNNNCQNGGPLVASGKVAAGVGGGGDTQTVLVTERREVAEGRESSKGNHVGEERRGYDMSRKRCSISVQQHGTDGTVVRTNYRELSPASLRIHRKSSHDIRNTLLGPDGEVLHLHDPSGKGGDGIAKMPAVVKPIKLKSLITKAESYDTMHGKATDIYCMSDYDISSIEHEALLLVVASTFGNGDPPENGELFKSGLETRKSKTLFAQDLYAMKLHESGHHQAHSELTIAASSKSFIKANSRSDLGKFGPMGGRKIDRLDSLRGSTTDTLSEETFGPLSNVRFAVFALGSSAYPNFCAFGKYIDNILGELGGERLMKMATGDEICGQEQAFRKWAPEVFKIACETFCLDPEETLSDAAFALQSELSENTVRYAPVSENEPLDRALSKFHNKKSMECSVKRNPINLHCEMNGTERSTILVEIMAEGIDYEPGDHVGIFPANRKEIVDGIIERLTGVNDPDEELQLQVLKEKQTQNGVYKSWEPHERLPVCSLRTLLTRFMDITTPPTRQLLTYLASCCSDKADEERLLMLANESSVYEDWRYWKLPHLLEVLEEFPSCRPPAAVFVAQLNALQPRFYSISSSPRKYSKEIHLTVAIVTYRAEDGEGAEHYGVCSNYLANLQPDDKIFLFVRSAPSFHMSKDPTRPVILIGPGTGIAPFRSFWQEWDHIKSEMVDCKIPKVWLFFGCRTKNVDLYRDEKEEMVQKGVLDRVFLALSREENIPKTYVQDLALKEADSISELILQEKAHIYVCGDVTMAEHVYQTLRKILATHENRTESEMEKYMLTLRDENRYHEDIFGITLRTAEIHNKSRATARIRMASQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WD10</accession>
    <name>A0A6E8WD10_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="16">PDZ domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON029508-RC">
        <property type="protein sequence ID" value="ACON029508-PC"/>
        <property type="gene ID" value="ACON029508"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002482"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON029508"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030142"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045197">
        <property type="term" value="P:establishment or maintenance of epithelial cell apical/basal polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098968">
        <property type="term" value="P:neurotransmitter receptor transport postsynaptic membrane to endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098887">
        <property type="term" value="P:neurotransmitter receptor transport, endosome to postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043113">
        <property type="term" value="P:receptor clustering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06704">
        <property type="entry name" value="PDZ1_Scribble-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06703">
        <property type="entry name" value="PDZ2_Scribble-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06702">
        <property type="entry name" value="PDZ3_Scribble-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06701">
        <property type="entry name" value="PDZ4_Scribble-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000585">
        <property type="entry name" value="Leucine-rich repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000599">
        <property type="entry name" value="Leucine-rich repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000064">
        <property type="entry name" value="protein lap4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000076">
        <property type="entry name" value="protein lap4 isoform X23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000041">
        <property type="entry name" value="protein scribble homolog isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000074">
        <property type="entry name" value="protein scribble homolog isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000153">
        <property type="entry name" value="Scribbled, isoform T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050614">
        <property type="entry name" value="Synaptic_Scaffolding_LAP-MAGUK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23119">
        <property type="entry name" value="DISCS LARGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23119:SF44">
        <property type="entry name" value="PROTEIN LAP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0965">Cell junction</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="10" id="KW-0221">Differentiation</keyword>
    <keyword evidence="8" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PDZ" evidence="15">
        <location>
            <begin position="669"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="15">
        <location>
            <begin position="844"/>
            <end position="934"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="15">
        <location>
            <begin position="1128"/>
            <end position="1218"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="15">
        <location>
            <begin position="1225"/>
            <end position="1317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="423"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="532"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="938"/>
            <end position="960"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="979"/>
            <end position="1051"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1065"/>
            <end position="1085"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1332"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1388"/>
            <end position="1408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1448"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1494"/>
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    <feature type="region of interest" description="Disordered" evidence="14">
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            <begin position="1623"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
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            <begin position="1817"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
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            <begin position="1871"/>
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    <feature type="region of interest" description="Disordered" evidence="14">
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
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        <location>
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        </location>
    </feature>
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        <location>
            <begin position="1666"/>
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    </feature>
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        <location>
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        <location>
            <begin position="1827"/>
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    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="14">
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            <begin position="1989"/>
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            <dbReference type="ARBA" id="ARBA00004202"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
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    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON029508-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="2125" mass="230806" checksum="0993A7ABCD477876" modified="2020-08-12" version="1">MFKYIPIFKGCNRQIEYVDNRHSSLPNVPEEIFRYSNSLEELLLDANHIRDLPKGFFRLYRLRKLGLSDNDIIKIPSDIQNFVNLVELDVSRNEIGDIPEDIKHLRSLQIADFSSNPISRLPAGFTQLRNLTVLGLNDMSLISLPQDFGCLSKLVSLELRENLLKNLPESISQLTKLERLDLGDNEIDELPSHVGYLPSLQELWLDHNQLLRLPPEIGLLKKLVCLDVSENRLEELPEEIGGLECLTDLHLSQNLLETLPNGISKLTNLSILKLDQNRLHTLNDSIGCCVHMQELILTENFLSELPATVGNMLVLNNLNVDRNSLVAVPSELGNCRQLGVLSLRENKLTRLPAELGNCGELHVLDVSGNLLQHLPYSLVNLQLKAVWLSENQSQPVPTFQPDVDETTNEQVLTCFLLPQQRYVPSNPSQREDTDSENWEEREASRTHSVKFTEDFGADRDTPFVRQNTPHPKELKMKAHKLFAKERKSDDLSGNLDTLSEESASRPSLLNRSGYGTATMEDIQGHAIPHDEEQGEAAAGSAEGGGGVEGAQDDEDGYEKRVGFEVNEDEDDDPERQQYGEDGDEHGGKKPISKLHRRDTPHHLKNKRVHHTITDKNTNLMFNSLKLKEMTPPKLDEHPAMEPPVPPSATASIAEDTLDTYTALKEERLEIHIERTSAGLGLSIAGGRGSTPFKGDDEGIFISRVTERGPADLAGLKVGDKVLKVNGISVEDADHYDAVEVLKACGSVLILYIAREVTRLIGHPVFDDTGSVAQISVSDQDALKPPPLVGAPLGLPTVDGAGVPPYMGALSPNTVGLPNGGAGGVGGGALDMPNGNEITQKIILHTTLIRDQIGQGLGFSIAGGKGHAPFKDGSEGIYISRLTENGVAHKDGKIMVGDRVLAINGVDITNAHHDYAVQLLTDHQRFVRLVVQREVKGPLEPLHSPRSPVVGPNRPPAAAAGAYLPNRLATTYTGYRRTAEDGQEQVAAAQQAHHHQHHQSPDSEPAAKVVEEQQQQQKVTGGANALSPSSSFAPHTSSNAQQQQQQQQPPLPAPRTVLMQSVNNKHGTANISSLPDGGTKGNGNGVIMHTQQQQQHHHHQEHPMEAPQVSATNNGVVAHTAATSDDPQEVILPKDQGSLGFSIIGGTDHSCTPFGANEPGIFISHIVAGGIAALSGKLRMGDRILKVNGTDVTQATHQEAVMELLRPCDDIKLTVQHDPLPAGFQEVHIVKQDGERLGMHIKGGLNGQRGNPLDNADEGVFISKINANGAAKRDGRLRVGMRILEVNGLSLLGASHQEAVDALRASGSKLHLVVCKGYEKSDLLHLAGSAGGMSTGLSNNGDSARIGSRASETGSELSQSVSSLDRDDSVIMAEAHRKLSVDKIDEETLPPQPAVHDHHPVDQPEPPTSQALISVNPNDQQVAIGPKEKSTPEKVLDIVRAAESLALGSQLVENGPPKSPTEVASEPLQKTTTIVMSKHTLDTQAPQVERRFGSVRSLSHANEPPISSINSSTATVATNPATTDTNYHHPPKGDQKIVRPHRPPPPPPPPLAAASTHTTTIPSPSAGAYLDRPPSTKPPPPPPKVEPGSSSSTTTTTSHRKSVSFDLHEDAHGFDTALHHARAAFAGPSPTPDLKGILRASSPSNRSSSSTPEGVQQVQPTVANLDSDPDTDSDTGTESDTDSDASGDSHELLPAAPDGQLAREQPPASNPIRRAEVVRNQAARKLQEEFGQGDLVEYEHDTATNTIREVSVGPGPAARGRPERVEMLPAKFETLPVKRTAQFVHENTPNNLLVPDEVHRQVLLEENEVRNKLLAQYAHEQQQQQQQQHHHHHQQQHMYTMHHHHHTGELLGRVPSPYHLYDSGSASVSPLPSHYIPGSSSSSVYDHHQHLGKLGGPLSPPPPLPSTAPPSDVGYHHHHLPQIYPPVQILPVHYTKLPTPQHTIYTYASGSVPVSHAPIVSLAASTSSQPVPYSSPYHLPPYAGTSAGPTYLSTAHPSSSIAAGPTHPHHQVLMPSPPLGPKTPKSVSDKKRFFENAMEDQQKPTPKSEKVFSFLSQDEVEKLRQEEERKIASLGKDRVHQRFGDEEDEDDDNRSDGGDRPPGDKSDINDNNMRNPLAEIDAVFRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WD20</accession>
    <name>A0A6E8WD20_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">P-type Cu(+) transporter</fullName>
            <ecNumber evidence="3">7.2.2.8</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="17">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.8" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8WD20"/>
    <dbReference type="EnsemblMetazoa" id="ACON029519-RD">
        <property type="protein sequence ID" value="ACON029519-PD"/>
        <property type="gene ID" value="ACON029519"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000618"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON029519"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032853"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043682">
        <property type="term" value="F:P-type divalent copper transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140581">
        <property type="term" value="F:P-type monovalent copper transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055070">
        <property type="term" value="P:copper ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015677">
        <property type="term" value="P:copper ion import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="CDD" id="cd02094">
        <property type="entry name" value="P-type_ATPase_Cu-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000150">
        <property type="entry name" value="ATP7, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000001">
        <property type="entry name" value="ATPase copper transporting beta"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000002">
        <property type="entry name" value="Copper-transporting ATPase 1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000031">
        <property type="entry name" value="Probable copper-transporting ATPase HMA5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017969">
        <property type="entry name" value="Heavy-metal-associated_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006122">
        <property type="entry name" value="HMA_Cu_ion-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027256">
        <property type="entry name" value="P-typ_ATPase_IB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01525">
        <property type="entry name" value="ATPase-IB_hvy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00003">
        <property type="entry name" value="copper ion binding protein"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43520">
        <property type="entry name" value="ATP7, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43520:SF8">
        <property type="entry name" value="P-TYPE CU(+) TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00942">
        <property type="entry name" value="CUATPASEI"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01047">
        <property type="entry name" value="HMA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0186">Copper</keyword>
    <keyword evidence="9" id="KW-0187">Copper transport</keyword>
    <keyword evidence="15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="16 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="413"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="455"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="489"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="518"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="678"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="723"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1132"/>
            <end position="1154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1160"/>
            <end position="1180"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="19">
        <location>
            <begin position="37"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="19">
        <location>
            <begin position="137"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="19">
        <location>
            <begin position="246"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="19">
        <location>
            <begin position="322"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="205"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="205"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU362081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON029519-PD"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1279" mass="138109" checksum="975F1608C41A4D95" modified="2020-08-12" version="1">MSLESKEFHVPADTQRLLRENMDDYGESTGLLEQPVASVRLPIIGMTCQSCVRNIEGTIGSKLGVIKINVVLAENAGYIDYDPSLTDPAQLAADIDDMGFECTYTEPGASSAGPKRSILVEGVESDSETTNQKSDVRTTRISIEGMTCQSCVRNIEGNMKDRPGVISIRVLLDERLGIVEYDGRQTTAEAIAEQIDDMGFEARVAGEQHQPEQQRARSSPQANGKKHATNGNLTPKKKADESALLRRCFLHVQGMTCASCVSAIEKHCRKIYGVESILIALLAAKAEVKYDERLTTPADVAKSITELGFPTEVLEEPGTGETDVEIEILGMTCGSCVAKIEQTALKIPGVLQASVALTLKRGRFKFNNERTGARTICEAIEGLGFATRVLSGKDKMAHNYLEHKEEIRKWRNAFLVSLVFGGPCMIAMVYFMVLMHERSHEEMCCVLPGLSLENLIMFTLSTPVQFVGGWHFYIQAYRAVKHGASNMDVLITMATTVSYLYSVGVLVAAMVLEQHTSPLTFFDTPPMLFIFISLGRWMEHIAKGKTSEALSKLLSLKATEATLVTLGPDYAVLSEKVISVDLVQRGDILKVVPGSKVPVDGKILCGNSTCDESLITGESMPVPKKKGAVVIGGSINQNGLLLMQATHTGEHTTLAQIVKLVEEAQTSKAPIQQLADRIAGYFVPFVVAVSVITLVGWIVSGYIDIGHIPASDRDKEGLTPSEIIVSYAFRCALSVLAIACPCALGLATPTAVMVSTGVGALHGILVKGAGPLENAHKVKTIVFDKTGTITHGMPMTSRICMLVKPAVCSLARALTIVGSAEVNSEHPIATAIVKYVKETLEIDGFGRCSNFSAVPGCGIRCVISNVGDTLKRVQQSEKMRNYQNSYRSEQQQQPLFMNGAVVEELIPQLSASQKNTIELQQLLHLEPIGEGPAGTGGDAGSGEAFADVNEYNVLIGNREWMARNAIVVPPEVNIRMSEEEQMGHTAILCAMNGQLVCMLSVSDMVKPEAHLAVYTLKRMGIEVILLTGDNKNTAASIARQVGINRVFAEVLPSHKVAKIQRIQEMGMRVAMVGDGVNDSPALAQADVGIAIASGTDVAAEAADVVLMRNDLLDVVACLDLSRKTVRKIHLNFLFASMYNLLGIPLAAGIFTPFGFTLEPWMASAAMAASSVSVVCSSLMIKLYKKPTRASLQTPEYVELMEAGAFLDPDTISVHRGLDDIPRPNLARSTSSTLAKLFGRGKDTKEDGLLATLDDDEFSVGIVKTSNASIYKDATELQKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WD23</accession>
    <name>A0A6E8WD23_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Cyclic nucleotide-binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8WD23"/>
    <dbReference type="EnsemblMetazoa" id="ACON029523-RA">
        <property type="protein sequence ID" value="ACON029523-PA"/>
        <property type="gene ID" value="ACON029523"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009177"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON029523"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032177"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034702">
        <property type="term" value="C:monoatomic ion channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005242">
        <property type="term" value="F:inward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000020">
        <property type="entry name" value="Potassium channel, voltage-gated eag-related subfamily H, member 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000011">
        <property type="entry name" value="Potassium channel, voltage-gated eag-related subfamily H, member 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1200.260:FF:000001">
        <property type="entry name" value="Potassium voltage-gated channel subfamily H member 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003938">
        <property type="entry name" value="K_chnl_volt-dep_EAG/ELK/ERG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003967">
        <property type="entry name" value="K_chnl_volt-dep_ERG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050818">
        <property type="entry name" value="KCNH_animal-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217:SF548">
        <property type="entry name" value="GH12235P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217">
        <property type="entry name" value="VOLTAGE AND LIGAND GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01463">
        <property type="entry name" value="EAGCHANLFMLY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01470">
        <property type="entry name" value="ERGCHANNEL"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0630">Potassium</keyword>
    <keyword evidence="6" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="165"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="207"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="312"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="380"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="405"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="15">
        <location>
            <begin position="508"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="664"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="886"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="678"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="707"/>
            <end position="718"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON029523-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="967" mass="109109" checksum="E2D3D39C940A27B8" modified="2020-08-12" version="1">MNKVYTEERKIKRSLSSCVMKMNRAHSQDQTNIPLIVTTNEIGAKPSSSTATSLDSAFKTKSCRSFVRQSKLSHEHRNKSVPQNLQAQEKWSSVRLSSDSKKNQIKIENHISTHSNLNLNGLSGDKANLGKEVLSLGADVLPEYKLQSPRVHKWTILHYSPFKAVWDWIILLLVMYTAIFTPYVAAFLLSEPDYNQRKNRKYADDPIVIIDLIVDVTFVVDILINFRTTFVNGQDEVVSHPGRIAVHYLSGWFLIDLVAAIPFDLLLVGSDTDELGLDKDETTTLIGLLKTARLLRLVRVARKIDRYSEYGAAVLVLLMATFALIAHWLACIWYAIGNAERPLLKAKIGWLDALAQDTQEYYFPNNTGGGPSVKSRYVTALYFTFTSLTSVGFGNVAPNTDAEKIFTICVMLVGSLMYASIFGNVSAIIQRLYSGTARYHTQMLRVREFIRFHQIPNPLRQRLEEYFQHAWTYTNGIDMNSVLKGFPECLQADICLHLNRNLLNNCSAFEAASPGCLRALSLKFKTTHAPPGDILVHKGDVLTYLYFIARGSIEILKDDVVMAILGKDDIFGENPCIHSTLGKSNSNVKALTYCDLHKIHRDDLLDVLDLFPEFYDSFVNSLEITYNMRDEEQAGVELRHRYMRTGSQDRESETRSYVRKLNTVHHRPPGNKCDMPNDRSSIGQMSTNYDDDRKFSLSASPNESPKSTHKQTIPHHHEHQSSAIVASSAALGRAPDTVTLSAVSHRACTCGSKADVPVGPMRVSDCSPKSSPDEEIFHHTAPNSSNMSKPDESKQNLETMAHQLSELTNRIGVLETSLKHDIRTILEILHQQQQMQMQIQHQQHSFAQQQQQMHQMHTGKTAMSSYQPSESDFSFDMCGPPMDCREVKHQQPPSSQHRIHVARSVSQPECTDDRSLFKCSKFSSFNHPMDDTPEGQNWNIFAPIAKLESLDEIDQETKPSSSHDKMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WD27</accession>
    <name>A0A6E8WD27_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat EMAP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040231659.1">
        <property type="nucleotide sequence ID" value="XM_040375725.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8WD27"/>
    <dbReference type="EnsemblMetazoa" id="ACON029525-RB">
        <property type="protein sequence ID" value="ACON029525-PB"/>
        <property type="gene ID" value="ACON029525"/>
    </dbReference>
    <dbReference type="GeneID" id="120955161"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004695"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON029525"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032386"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21931">
        <property type="entry name" value="TD_EMAP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001322">
        <property type="entry name" value="Echinoderm microtubule-associated protein-like CG42247"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000005">
        <property type="entry name" value="Putative echinoderm microtubule-associated protein-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055442">
        <property type="entry name" value="Beta-prop_EML-like_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055439">
        <property type="entry name" value="Beta-prop_EML_1st"/>
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        <property type="entry name" value="Elp-1-like_TD"/>
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        <property type="entry name" value="HELP"/>
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    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
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    <dbReference type="InterPro" id="IPR050630">
        <property type="entry name" value="WD_repeat_EMAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13720:SF50">
        <property type="entry name" value="ECHINODERM MICROTUBULE-ASSOCIATED PROTEIN-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13720">
        <property type="entry name" value="WD-40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23409">
        <property type="entry name" value="Beta-prop_EML"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03451">
        <property type="entry name" value="HELP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 8" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="EML-like first beta-propeller" evidence="10">
        <location>
            <begin position="350"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="627"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="domain" description="EML-like second beta-propeller" evidence="11">
        <location>
            <begin position="634"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="710"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="756"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="868"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="76"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="171"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="218"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="84"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="108"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="171"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="225"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
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    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF23414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON029525-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="903" mass="98279" checksum="293CCE3AE0C4C8BD" modified="2020-08-12" version="1">MSAVQPVLSTADTAKKTMDVSNGSKEWQEMLETEKAGLVERVYDLERQVLEHKDEIVCLKSTLADVLRRLATIDNSYENSTSGSGRGGTGTPAAGGAGGGSGGHHTVSDNVSASGQNSLSSKSFRFSRNTLTRLNSTVDEKRFGRVPSRVTSSPSRVKTNLAQKAIHYSNGSLHSDSMSSHSISPAPSPSPRQPNASAVANSHHALLASSGGMGKRWSSTGDFISTPGSPSGTGSSGSMSRFSAKSLLNLSGNSSRQGQTHPYVQRKNEDDEYVKLYISGRPIVIHLPEAQLSTYELTKVQPAPNRRLRLDWAYGYRGKDCRSNLYQLPTGEMVYFVAAVVILYNMDEHTQRHYLGHTDDVKSLAVHPNKLLVASGQCGGHEGRDSLPHILIWNSVSLATLNMIGCGEFTGSINCLSFSRADSGSILAAIDDSPDKIISIWDWQKKEGGRKITETKCSVDTVVAVEFHPLDKGQIITIGKNHIAFWSLDQNGMLYKRMGVFESFEKPKYVTAISFTQTGDVVTGDSSGNLAVWKRGTNVISRFLKKVHEGPVFSICALRNGGFVTGGKDGLLLLFDDALHIKAEQIVEAHFGAVRVVAEGKGSQLLIGTTKNCILSGDFTLSLVPIVMGHTDILWSLATHPQVAQFVTGGRDRLLQLWDSLSHSVVWSKDIGEPIHAVQIANAGDVIVAGGVGGRWSVFDIVTRELLATYTDGQEVIQCMQFSPDGNLLAVGSKDNCIYIYQCTKVAHRFSKIGKCTGHSSFISHLDWSKDSQVLRSNSGDYEILYWNPTLCRQITSQSTVKNLEWATQNCSVSFETIGIWPENFDGTDINSVCKDGEEQFLVCADDFGKIRLFSFPASQPKSLSHSYRGHSSHVTAVQFMHDGVRLLSAGGMDTSVLQWRVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WD53</accession>
    <name>A0A6E8WD53_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Stress-activated protein kinase JNK</fullName>
            <ecNumber evidence="11">2.7.11.24</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, and thus regulates transcriptional activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.24" evidence="11"/>
    <dbReference type="RefSeq" id="XP_040230935.1">
        <property type="nucleotide sequence ID" value="XM_040375001.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040230936.1">
        <property type="nucleotide sequence ID" value="XM_040375002.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040230937.1">
        <property type="nucleotide sequence ID" value="XM_040375003.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040230938.1">
        <property type="nucleotide sequence ID" value="XM_040375004.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040230939.1">
        <property type="nucleotide sequence ID" value="XM_040375005.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040230940.1">
        <property type="nucleotide sequence ID" value="XM_040375006.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_049466493.1">
        <property type="nucleotide sequence ID" value="XM_049610536.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_049466494.1">
        <property type="nucleotide sequence ID" value="XM_049610537.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_049466495.1">
        <property type="nucleotide sequence ID" value="XM_049610538.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_049466496.1">
        <property type="nucleotide sequence ID" value="XM_049610539.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8WD53"/>
    <dbReference type="EnsemblMetazoa" id="ACON029555-RB">
        <property type="protein sequence ID" value="ACON029555-PB"/>
        <property type="gene ID" value="ACON029555"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON029555-RC">
        <property type="protein sequence ID" value="ACON029555-PC"/>
        <property type="gene ID" value="ACON029555"/>
    </dbReference>
    <dbReference type="GeneID" id="120954773"/>
    <dbReference type="CTD" id="44801"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012096"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON029555"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033372"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036477">
        <property type="term" value="C:somatodendritic compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903034">
        <property type="term" value="P:regulation of response to wounding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07850">
        <property type="entry name" value="STKc_JNK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000009">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000210">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003527">
        <property type="entry name" value="MAP_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008351">
        <property type="entry name" value="MAPK_JNK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01772">
        <property type="entry name" value="JNKMAPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01351">
        <property type="entry name" value="MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 11" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="23"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU368052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON029555-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="375" mass="43130" checksum="ADC751D0D33C3924" modified="2020-08-12" version="1">MSSRHAFYTVEVGDTKFTILKRYQNLKPIGSGAQGIVCAAYDTVTQQNVAIKKLSRPFQNVTHAKRAYREFKLMKLVNHKNIIGLLNAFTPQRTLEEFQDVYLVMELMDANLCQVIQMDLDHERMSYLLYQMLCGIKHLHSAGIIHRDLKPSNIVVKSDCTLKILDFGLARTAGTTFMMTPYVVTRYYRAPEVILGMGYKENVDIWSVGCIMGEMIRGGVLFPGTDHIDQWNKIIEQLGTPSQNFMTRLQPTVRNYVENRPRYSGYAFDRLFPDVLFPTDSNEHNRLKASQARDLLSRMLVVDPEHRISVDQALVHSYINVWFDESEVNAPAPGPYDHSVDEREHTVEQWKELIYQEVMEYEARNNLADGEGAPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WD58</accession>
    <name>A0A6E8WD58_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Serine-rich adhesin for platelets</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="EnsemblMetazoa" id="ACON029561-RA">
        <property type="protein sequence ID" value="ACON029561-PA"/>
        <property type="gene ID" value="ACON029561"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012835"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON029561"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042479"/>
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        <property type="component" value="Unplaced"/>
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    <proteinExistence type="predicted"/>
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    </feature>
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    </feature>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1466" mass="156967" checksum="2171C0DBA586772D" modified="2020-08-12" version="1">SSTSMEVASLATGTGTTTATRAPFQREVREWQHVDPNTGALLTGRLEADRWVNGPLNSYGKMVSQNLSTPDGTQHTQRKQMEILQARTSAGSLQVVRAQTVQTTSSRWSSSTVHSTTTSTEQTTGSSGSVGAITGPTAAGSPLATNGFSSHHLLSSFHRPLSSGTTSSSRLLTVGSSRSDAPQTTPLGGSAFVSTATKPFPRFSSSPTSAGSSFVNTSGPLRYGRQLYAVDADAHDDRGSYHHTATNQQPLRTVAVEQDSSINGNSVPLARGKSISIEDLSTERDESNFDDDVEPTQWKRVSKIRRSLQFPRKTTPRTNTRPIDLPENSVSVSRICQELENGRRLNTAMRNNHIDFVALDNILKSVADQPCTAATTPPHSNPTHSKSPPKASFLTAESLREIRGRLRRLSNESLYRDDMSPADDASGQTVASDRPSPADEGGVTITEVETRLGQLETSSSSDSNYRSSSLQTSKKPAPDTTVLAPDDWHSRRKSYGFERMSQQPLGSGFAMAKMDSSTDSGIGRSSDLSSSWSSAATESGQGAGGSYHHQRATIVTLGERSQLKEKQATNPAMVIKITSKPADSTSDTFAVPSLEPTDEEPKRHSIAVDETSYVRDSLRTMFERKSSVHVNGFSHTLIDDLSRNKKRVEFCKTEVHFTADSGRVNIVETDEKPPPTNNFRRRRRSSGGSSYLVSNLDGDSDSVNATDESKLVGPSSESVVIEQDTAPVVTTTIGGMGRTVPEPTPRKYVPSATVTVSDESDGHSTDEISLRSILKNKPVKPKPYVLGENLDSNEALWGVKLKPVVAGGATVEQSQPRLLSVEMGTESKSSSPIAAGSGYSTRINLTKEPAGPSSWSGSNSTEFDVVKPSALISRQPAKPSAVMSIELKSPTPVKKLPPASHTSSPDTSTTKITTTLNDESTAASTQQQSPKFRRYIPTTATSATTPGAVRNEERASLRLSSGARQLRENELSFFGVNPNKQQSSSPAGPTGKPPTTSTTTPNSPFQRSATLTSSFIRRSGTTTTSSTTTTTGSGDSATSKTSLWQLTNDKPDLLRHGQQAAAAVEQPKSQAVAAPRPAVIRTGSSSVATSNSVSVNSSSSSVTTSTLSSADGSPHYENLSHFALKPKAAQRSVGSSPSAGELLVQQQTAKVAESEKPAAAYDRKKDLERDERILEELTRAADEILNVVNNMSNGESMESILSELNDGEGHYRRLSTGGCTLGTIRECSTTNKIMKSRGVQVSKNIDDSLKRHHQRQSRLSSASSNESIGRRPEGNFTRSVSLRGPSTASTTAGDSLSSRSGSSRRSRAPMATETRRLVRMCSKEKLQSNASSSEDLPSTTVVEPPRRPRRTRYHSKETKDPERTSGTSNGHGTRVSSSTRSHRSERSSGSIRTVSSRHQGQSATPTTGHTAQTAVSASVSSHHSLHSASCPSSRGLKSSATNGHHQHHHSQLASATTTTSSKKHHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
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        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
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        <lineage>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
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            <taxon>Diptera</taxon>
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            <taxon>Culicoidea</taxon>
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        </lineage>
    </organism>
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        <citation type="submission" date="2023-03" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
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        <property type="nucleotide sequence ID" value="XM_040362430.2"/>
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        <property type="gene ID" value="ACON004631"/>
    </dbReference>
    <dbReference type="GeneID" id="120947261"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_036458"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR052110">
        <property type="entry name" value="MCFD2-like"/>
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    <dbReference type="PANTHER" id="PTHR23104:SF17">
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    <dbReference type="PANTHER" id="PTHR23104">
        <property type="entry name" value="MULTIPLE COAGULATION FACTOR DEFICIENCY PROTEIN 2 NEURAL STEM CELL DERIVED NEURONAL SURVIVAL PROTEIN"/>
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    </dbReference>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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            <dbReference type="ARBA" id="ARBA00022837"/>
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            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50222"/>
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    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="217" mass="25632" checksum="D085A089F381B506" modified="2023-06-28" version="1">MKHSVLITLAILMQLASLAICQRVAPGVNPNHYQQQQQYQPPPPQYQQQAPQHQQPQYQQQPPQQQQQPQYQQQQFQQQQQHQQPHGEHGQQQVLHTGNLQQEKQHIAEHMEVPIDTSKMSEQELQFHYFKMHDSDNNNKLDGCELIKSLIHWHAEDKTGHHEEQAPRPEYTDEQLEDIVDSVLRMMDVNTDGFIDYTEYRMSGVEENRRDDFPEGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9I3BD11</accession>
    <name>A0A9I3BD11_ANOCL</name>
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            <fullName evidence="2">Uncharacterized protein</fullName>
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    </protein>
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        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2023-03" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A9I3BD11"/>
    <dbReference type="EnsemblMetazoa" id="ACON030710-RA">
        <property type="protein sequence ID" value="ACON030710-PA"/>
        <property type="gene ID" value="ACON030710"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_036950"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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            <begin position="1"/>
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            <begin position="856"/>
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        <location>
            <begin position="87"/>
            <end position="108"/>
        </location>
    </feature>
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            <begin position="127"/>
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        </location>
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    <feature type="compositionally biased region" description="Pro residues" evidence="1">
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            <end position="162"/>
        </location>
    </feature>
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        <location>
            <begin position="180"/>
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            <begin position="216"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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            <begin position="257"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="330"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="349"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="383"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="393"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="476"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="499"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="555"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="579"/>
            <end position="612"/>
        </location>
    </feature>
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        <location>
            <begin position="767"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="810"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="863"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="968"/>
            <end position="985"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1018"/>
            <end position="1028"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON030710-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1028" mass="109811" checksum="AFBA3A71FDDCFC05" modified="2023-06-28" version="1">MDRSSYGGPPQSTIGNSARRIQAQLPAEPPACVQNAMMRRDKQPFTYTPGGIDLSQIKSPRMAKRISRNANSEGVTGQPKVSPLAQNNGSSYSSNGTSSSNGNTSSVSPAATLGAAAMGMPFQVFPTGPPAPPPPPPPTGRVLPSNGGNRSIPPPPPPPGPAQPTASSSDARKSPRPQSFEPPPMGMRPEIKIPPNPMAALRKAPRPQPKNDFWIEEYRREKQQDERPQQIPRPVPPSYSYTNRDLDDEVAKNTFNRTDELTVRGDHPLAQHHHRSPSPSNLTVPHESGSRGSSSIKTPPVYGQSPVPANLPPPITPTKVSAHPPATKDSPPPPPPPVRPKPSLSPVSTSNQYSGSTTSTPLHQPGGGSAGKEQTAGVKAAAAPPPPPPPPHASSASSSHKDTAQSSGVSPSVSSASSSGSPNQSPTSAKPTSTQRPAAQALGSLYIPPIHEVFANSKQSLLTQASPPWMSSRHNSLKEQPEWVHKDEQDSTTNTNATQSPEQSVEQEQSSKPPASSTSGSPRAPSVAKESVAATSKPVDSIVTAPTAVSARQAVPSQPLTPNSNRSAAPAADVSNPVQHQQTGTASGQQQQQQYGSQQQQQQQQTGRATGGLPAAGTVSSTVTMPVNRGYPQQAPVYAQAQPVYSTQSPAGTQKERIIPIQIEKSPVQAPVTAPTFAPPPYYSPSAQYGPASAQSSMQSPIAVGFATPHSGFTTPTAMFTNPNHFVNQGYNNISYPPTPTGQPHPHYQHHQHYAPPQTPPMHHPMHPQAQQQRMQQSTPTQQGQQVTNVRIVPIKVESGSDHTTVRGPLSNTPAIIQSDPRSSSNAQSWNGNNAPNQSRSFRVLQQITDTLDEAEAEKNAKEQSNNQSDQADGAKQQYSRSTAQQQDAAEGQLRRLQLSNEDKALMNRVKNQVDGEVYFHNEEDPRYRGAAIPSKAFRYLQNMTDSGQAANQSNNGSGNRAQQMFNKANYNDSDGDTQQSQQYVPPSEQKVEEPKKYTGGSIPSRSFKMLQAMTDAPDQRSDAKRKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9I3BD31</accession>
    <name>A0A9I3BD31_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="13">RING-type E3 ubiquitin transferase</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2023-03" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_040233230.1">
        <property type="nucleotide sequence ID" value="XM_040377296.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9I3BD31"/>
    <dbReference type="EnsemblMetazoa" id="ACON030722-RB">
        <property type="protein sequence ID" value="ACON030722-PB"/>
        <property type="gene ID" value="ACON030722"/>
    </dbReference>
    <dbReference type="GeneID" id="120955986"/>
    <dbReference type="CTD" id="114088"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041012"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19803">
        <property type="entry name" value="Bbox1_TRIM9-like_C-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19764">
        <property type="entry name" value="Bbox2_TRIM9-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16576">
        <property type="entry name" value="RING-HC_TRIM9-like_C-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12889">
        <property type="entry name" value="SPRY_PRY_TRIM67_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000009">
        <property type="entry name" value="E3 ubiquitin-protein ligase TRIM9 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000178">
        <property type="entry name" value="E3 ubiquitin-protein ligase TRIM9 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
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    <dbReference type="InterPro" id="IPR003649">
        <property type="entry name" value="Bbox_C"/>
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    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017903">
        <property type="entry name" value="COS_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050617">
        <property type="entry name" value="E3_ligase_FN3/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24099">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM36-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM9"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
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    </dbReference>
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        <property type="entry name" value="zf-RING_UBOX"/>
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    </dbReference>
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        <property type="entry name" value="BBC"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51262">
        <property type="entry name" value="COS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="B box-type" evidence="9">
        <location>
            <begin position="186"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="9">
        <location>
            <begin position="239"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="COS" evidence="12">
        <location>
            <begin position="388"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="11">
        <location>
            <begin position="452"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="10">
        <location>
            <begin position="527"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="79"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="79"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON030722-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="718" mass="78382" checksum="1E215F55B5EE3951" modified="2023-06-28" version="1">MEEELRCPVCKQLYASPVLLPCYHALCLACALDIQTASSSSSSTAGSSSSSTASSTSSAGHPPCHPAIAAAHLHQSHSQHSSAASTGSSSATESVSSDQDQADKVSILSEADSGVICCTSRPGSYAGTPNLQGLLFPPAGSGGSVYSLACPVCRKLVFFDELGARNLPLYRAMESIVDRFCEREALRCQMCETEPPKVATVICEQCEIRYCDACRELCHPARGPLAKHSLTKPRGACQLRESICGEHTEPLTMYCMGCKLPICNQCIGDNRHQSHDLQSITAMCKAQKTELSHNLQQLSEKARSTTEFIQRLKGMSDKVTDSCIEFERLVTVQCEALIAAINARRDVLLDVIRSDKEAKIRTLKDQQASCTGKLQQTTGLIQFCIEALKETDSAAFLQVGSMLINRVANTDMTWHQEVTNAAPRVSPIIDLTLDDSSVIRAIDNLNFIQMKLPLAPVIIPEDCSAENNSVTVAWQAPSHSFVQGYVLELDDGNGGEFREVYCGKETICTVDGLHFNSMYNARVKAFNNTGEGEYSELIGLQTAEVAWFTFDSVLSGGPCSGLNFSSENQTVSADGWEHRVALGSVGFSRGVHYWEFTIDKYTADTDPAFGVARLDVARDKMLGKDDKGFAMYIDRQRSWFQHNSVHERRVEGGISTGSTVGVLLDLERHTLHFIVNEMPQGSVAFRDLYGVFYPAVSVNRGVTLTLHTGLDAPRMDYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9I3BD86</accession>
    <name>A0A9I3BD86_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Pyruvate kinase</fullName>
            <ecNumber evidence="17">2.7.1.40</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2023-03" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Pyruvate kinase that catalyzes the conversion of phosphoenolpyruvate to pyruvate with the synthesis of ATP, and which plays a key role in glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>pyruvate + ATP = phosphoenolpyruvate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18157"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.40"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="15">
            <dbReference type="Rhea" id="RHEA:18159"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 17">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 17">Belongs to the pyruvate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.40" evidence="17"/>
    <dbReference type="RefSeq" id="XP_040230483.1">
        <property type="nucleotide sequence ID" value="XM_040374549.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9I3BD86"/>
    <dbReference type="EnsemblMetazoa" id="ACON030709-RB">
        <property type="protein sequence ID" value="ACON030709-PB"/>
        <property type="gene ID" value="ACON030709"/>
    </dbReference>
    <dbReference type="GeneID" id="120954020"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038201"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:magnesium ion binding"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:pyruvate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00288">
        <property type="entry name" value="Pyruvate_Kinase"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Pyruvate kinase"/>
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        <property type="entry name" value="Pyruvate kinase"/>
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        <property type="entry name" value="Pyruvate kinase"/>
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        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
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        <property type="entry name" value="PK beta-barrel domain-like"/>
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    </dbReference>
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        <property type="entry name" value="PRK05826.1"/>
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    </dbReference>
    <dbReference type="NCBIfam" id="NF004978">
        <property type="entry name" value="PRK06354.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01064">
        <property type="entry name" value="pyruv_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11817">
        <property type="entry name" value="PYRUVATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00224">
        <property type="entry name" value="PK"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01050">
        <property type="entry name" value="PYRUVTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF50800">
        <property type="entry name" value="PK beta-barrel domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52935">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00110">
        <property type="entry name" value="PYRUVATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13 17" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="9 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0630">Potassium</keyword>
    <keyword evidence="14" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 17" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyruvate kinase barrel" evidence="18">
        <location>
            <begin position="31"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate kinase C-terminal" evidence="19">
        <location>
            <begin position="398"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00058419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF02887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON030709-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="518" mass="56271" checksum="9EE252C461F3530A" modified="2023-06-28" version="1">MEGKKMNPEAVSHLEHICSLDIDSKTPFVRLSGIICTIGPASVSPEMLEKMMATGMNIARLNFSHGSHEYHANTIKNIREAVDNYSKKLGKPFPLAIALDTKGPEIRTGLIEGSGTGEVELKKGEKIQLTTDKEHLEKGTKDKIYVDYVNIVKVVKPGDHVFVDDGLISLVVESISGDTLTCTVENGGMLGSRKGVNLPGVPVDLPAVSEKDKSDLAFGVEQGVDVIFASFIRNAAALKEIRTILGEKGKHIKIISKIENQQGMQNLDKIIEATDGIMVARGDLGIEIPAEKVFLAQKSMIARCNRAGKPVICATQMLESMIKKPRPTRAEISDVANAIIDGADCVMLSGETAKGEYPLECVLTMAKTCKEAEAALWHRNLFKDLVDTTPTPLDTAASIAIAGAEASIKSRAAAIIVITTSGRSAHLISKYRPRCPIIAVTRFAQTARQCHLYRGILPVIYEQPAMEDWLKDVDARVQYGIEFGKERGFLKPGNPIVVVTGWKQGSGFTNTIRVINVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8UYG3</accession>
    <name>A0A6E8UYG3_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="10">TEA domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8UYG3"/>
    <dbReference type="EnsemblMetazoa" id="ACON000512-RA">
        <property type="protein sequence ID" value="ACON000512-PA"/>
        <property type="gene ID" value="ACON000512"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000895"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000512"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034347"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048568">
        <property type="term" value="P:embryonic organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035329">
        <property type="term" value="P:hippo signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.50.80:FF:000001">
        <property type="entry name" value="Transcriptional enhancer factor TEF-1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.50.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.20.40">
        <property type="entry name" value="TEA/ATTS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000818">
        <property type="entry name" value="TEA/ATTS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038096">
        <property type="entry name" value="TEA/ATTS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050937">
        <property type="entry name" value="TEC1_TEAD_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016361">
        <property type="entry name" value="TEF_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041086">
        <property type="entry name" value="YBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11834:SF0">
        <property type="entry name" value="PROTEIN SCALLOPED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11834">
        <property type="entry name" value="TRANSCRIPTIONAL ENHANCER FACTOR TEF RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01285">
        <property type="entry name" value="TEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17725">
        <property type="entry name" value="YBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002603">
        <property type="entry name" value="TEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00065">
        <property type="entry name" value="TEADOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00426">
        <property type="entry name" value="TEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00554">
        <property type="entry name" value="TEA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51088">
        <property type="entry name" value="TEA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TEA" evidence="9">
        <location>
            <begin position="76"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="TEA" evidence="7">
        <location>
            <begin position="76"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="39"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR002603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000512-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="417" mass="47186" checksum="C6875FAA47513ADA" modified="2020-08-12" version="1">MKIEHEADDCKLLQYKRQLYLDSGLGASTITARWTQMGPGPTAGSLVPDDTNGSGVDGGKHLDVGDMSDDEQDLSSADAEGVWSPDIEQSFQEALAIYPPCGRRKIILSEEGKMYGRNELIARYIKLRTGKTRTRKQVSSHIQVLARRKLREFQAKMKVQFWQPGLQPSTSQDVKPFAQPGYPMKTATAVSEGALQPAHPWEGRAIATHKFRLVEYSAYLELRAEETYHKHLFVHIADTPANPLLESVEVKEIYDKFPQKSGGLKELYEKGPSNAFFLVKFWADLNTNIANDAGAFYGVSSHYESNDNMVITCSTKVCSFGKQVVEKVETEYSRIENGRYVYRISRSPMCDYMINFINKLKHLPEKYMMNSVLENFTILQVISNKDTDETLLCVAFVFEVSTSEHGAQHHIYRLVKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V035</accession>
    <name>A0A6E8V035_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Microtubule-associated protein Jupiter</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds to all microtubule populations.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the MAP Jupiter family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8V035"/>
    <dbReference type="EnsemblMetazoa" id="ACON001372-RC">
        <property type="protein sequence ID" value="ACON001372-PC"/>
        <property type="gene ID" value="ACON001372"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004269"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001372"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042882"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033335">
        <property type="entry name" value="JUPITER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34930">
        <property type="entry name" value="GEO05313P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34930:SF2">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEIN JUPITER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17054">
        <property type="entry name" value="JUPITER"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="38"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="60"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="128"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="150"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="211"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001372-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="248" mass="25707" checksum="26D366EDE26EA32E" modified="2020-08-12" version="1">MTSTNFTVGFGENSKASSKVLKPPGGGSSDLFGADQLGPNTPRTMSKNRMQSNIFAAPSDAHKNGSETPRRNGQGQDSHNRLFGEVNRPFTPAKNHMKSNLPIGNPGDSTDGAKPHQNGKANGVAHQNGNGTANGHHTNGNGHTNGNGHISSASSDNGSSHTSPRSPRSPRFAQRNPVTGNGVEDYADKPRKLSSRRGAGDGNPVTGEGYSKSNGAAEINTTVPSLNGGGHVINKNRIPPGGFSSGLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V242</accession>
    <name>A0A6E8V242_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8V242"/>
    <dbReference type="EnsemblMetazoa" id="ACON000483-RA">
        <property type="protein sequence ID" value="ACON000483-PA"/>
        <property type="gene ID" value="ACON000483"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006804"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000483"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040123"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045334">
        <property type="term" value="C:clathrin-coated endocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043812">
        <property type="term" value="F:phosphatidylinositol-4-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001135">
        <property type="term" value="P:regulation of endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002013">
        <property type="entry name" value="SAC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45662">
        <property type="entry name" value="PHOSPHATIDYLINOSITIDE PHOSPHATASE SAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45662:SF8">
        <property type="entry name" value="PHOSPHATIDYLINOSITIDE PHOSPHATASE SAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02383">
        <property type="entry name" value="Syja_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50275">
        <property type="entry name" value="SAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAC" evidence="1">
        <location>
            <begin position="197"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000483-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="625" mass="69520" checksum="D0C217232BEDC1E8" modified="2020-08-12" version="1">MAMEVFQTETHYIFVRKERSLWWNRYSSEFQLKCGWDLSSVDDIECIGVTHGIVGTIALQGVLDPHLIVIKEVVPVGVLYAPHLVYKIRSIAVLGPDGADTVLLGCSKHTTSQTVKVAPAGGTAAPEGHPSPAKGRLFESSALVNKTWGAVKSAGSTIRNTTEKAAAIATSQVKSTVSLVVKADPVRIEKRVLDELHKIFDETDSFYYSPNCDITNNLQRRGAPPDDRFYWNRPMLAELLRVVSEQPGGEDWVVPIIQGFVQVEQCVIGNECFTLALVSRRSRNRAGTRYKRRGVDEHGNCANYVETEQVLSLRQHQISFTQVRGSVPVYWSQPGYKYRPPPRLDRDEPETQAAFERHFDGELQIYQSICIINLVEQSGKEKIIGDAYANHVLKYNSDRLIYVTFDFHEYCRGMRFENVSSLIESLAPEAGAMGFHWRDTNGPICNQKGVFRVNCMDCLDRTNVVQTAIGKAVLESQLVKLGLAMPYSQLPDALKAPFMVLWANNGDVISRQYAGTNALKGDYTRTGERKISGIMKDGMNSANRYYLARFKDTYRQATIDMMLGNNVSPESVSALGGTQTAATDETDALEGAEHARLLVEDCRRLLLGTAQLPVGAWGLIDADPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V267</accession>
    <name>A0A6E8V267_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="32">Protein deacetylase HDAC6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="33">Tubulin-lysine deacetylase HDAC6</fullName>
        </alternativeName>
    </protein>
    <organism evidence="37 38">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="37" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="37">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="31">
            <text>N(6)-acetyl-L-lysyl-[alpha-tubulin] + H2O = L-lysyl-[alpha-tubulin] + acetate</text>
            <dbReference type="Rhea" id="RHEA:21548"/>
            <dbReference type="Rhea" id="RHEA-COMP:11278"/>
            <dbReference type="Rhea" id="RHEA-COMP:11279"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="31">
            <dbReference type="Rhea" id="RHEA:21549"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>N(6)-acetyl-L-lysyl-[histone] + H2O = L-lysyl-[histone] + acetate</text>
            <dbReference type="Rhea" id="RHEA:58196"/>
            <dbReference type="Rhea" id="RHEA-COMP:9845"/>
            <dbReference type="Rhea" id="RHEA-COMP:11338"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="3.5.1.98"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="30">
            <text>N(6)-acetyl-L-lysyl-[protein] + H2O = L-lysyl-[protein] + acetate</text>
            <dbReference type="Rhea" id="RHEA:58108"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="30">
            <dbReference type="Rhea" id="RHEA:58109"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="8">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell projection</location>
            <location evidence="7">Axon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cell projection</location>
            <location evidence="4">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Cilium basal body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
            <location evidence="5">Cytoskeleton</location>
            <location evidence="5">Microtubule organizing center</location>
            <location evidence="5">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Perikaryon</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the histone deacetylase family. HD type 2 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040222304.2">
        <property type="nucleotide sequence ID" value="XM_040366370.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040222311.2">
        <property type="nucleotide sequence ID" value="XM_040366377.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040222321.2">
        <property type="nucleotide sequence ID" value="XM_040366387.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040222332.2">
        <property type="nucleotide sequence ID" value="XM_040366398.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8V267"/>
    <dbReference type="EnsemblMetazoa" id="ACON000532-RA">
        <property type="protein sequence ID" value="ACON000532-PA"/>
        <property type="gene ID" value="ACON000532"/>
    </dbReference>
    <dbReference type="GeneID" id="120949221"/>
    <dbReference type="KEGG" id="acoz:120949221"/>
    <dbReference type="CTD" id="10013"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_014543"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000532"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_029906"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000118">
        <property type="term" value="C:histone deacetylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043204">
        <property type="term" value="C:perikaryon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141221">
        <property type="term" value="F:histone deacetylase activity, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040029">
        <property type="term" value="P:epigenetic regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051646">
        <property type="term" value="P:mitochondrion localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051129">
        <property type="term" value="P:negative regulation of cellular component organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032886">
        <property type="term" value="P:regulation of microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd10002">
        <property type="entry name" value="HDAC10_HDAC6-dom1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000342">
        <property type="entry name" value="Histone deacetylase 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.800.20:FF:000005">
        <property type="entry name" value="histone deacetylase 6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="HDACs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001607">
        <property type="entry name" value="Znf_UBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625:SF38">
        <property type="entry name" value="HISTONE DEACETYLASE 6, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02148">
        <property type="entry name" value="zf-UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00290">
        <property type="entry name" value="ZnF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50271">
        <property type="entry name" value="ZF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="25" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="28" id="KW-0966">Cell projection</keyword>
    <keyword evidence="22" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="26" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="19" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0488">Methylation</keyword>
    <keyword evidence="27" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="38" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0678">Repressor</keyword>
    <keyword evidence="24" id="KW-0804">Transcription</keyword>
    <keyword evidence="23" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="14" id="KW-0808">Transferase</keyword>
    <keyword evidence="21" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="18" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="20" id="KW-0862">Zinc</keyword>
    <keyword evidence="17 34" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBP-type" evidence="36">
        <location>
            <begin position="988"/>
            <end position="1088"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="35">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="35">
        <location>
            <begin position="921"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="35">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="35">
        <location>
            <begin position="925"/>
            <end position="958"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00004489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00007738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00048287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00049136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00050910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00068733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="ARBA" id="ARBA00082852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="35">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="36">
        <source>
            <dbReference type="PROSITE" id="PS50271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="37">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000532-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="38">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1114" mass="123692" checksum="A3169613092C8676" modified="2020-08-12" version="1">MNPNVVTRSSAKQSKVRTRAQQAATEAEQEERRVKLYGGYRSTGKGSIAEAKKQGKLRMSATSQNGTGAGGDQRRSSDFTEVQLKDIFQNAQSGARTVRGPTGILYDERFALHRCLWDTNYPECPERYLRVMERFTIGQLVDRCQFIAPRPATRAELLTKHTAEQIELLAQTHGSQDEPELERLSSHYDAIFIHPTSYETSVLAVGSTIQLVESVWSGTVQNGMAILRPPGHHAMTAEYNGYCFFNNVAIAAQHALDHLGTQKILIVDWDVHHGQGTQRMFYSDPRVLYFSIHRYEHGTFWPNLRESDFDYVGTGKGEGYNFNVPLNKTGMTNGDYLAIWQQLLLPVATEFQPELIIVSAGYDAAYGCPEGCMEITPAFYSHLLSPLLLMAQGRVAVVLEGGYCLESLAEGCALTLKTLLGDPAPRLAEALQPPSESMQASILNCIYSHRKYWKCLQLYELYDLEDYNNTNPQDNFHKVIKCYVPPEPPAQPGRYETRNCYPVQSADERARIQDRLARLHLATDLSFPPDRVCYVYDELMLEHRNSAEKWHPEQPERIAKIMQRLTGDYQLAQRMKRLPGRLATTEELCLVHGPEHVRLIGEVCQSSTMKQTADQYNSIYFHPSTDASARMATGSVLTVVEDVLEGRTRSGVCVVRPPGHHAEADTPHGFCIYNSIAVAARHAVKRYGLRRVLIVDWDVHHGNGTQHIFEQDPHVLYVSVHRYDNGAFFPRSTDADYTVVGSGPGRGFNVNIPWNRKGMGDPEYVAAFHSVILPIAYEYEPELVLVSAGFDAAIGDPLGGCRVTPEAYGHFTQWLSVLAGGRLVLCLEGGYNVNSISHAMAMCAKALLGDPLPMLLPVSGTARTPPATHASCCETLSNVLSVQRLYWRSLCFNKKLPSSEPLLEETGSELIEALDNMTLSERGGTDQQQPQQTTSTPSSSSSAATAGSSGGEQAAGSSKQPSQTLTEFLEANLEALQNEEMFAVIPRRDCPHLRLLRPETAPEAIDTRTPCSDCGAEVENWICLLCFGVYCGRYVNEHMLRHGTGAADHPLALSFADLSVWCYGCDAYIDHPALHHYKNLVHQDKFHEPLVWSYGSDLVLDVVPGSAGRKAGQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V3W0</accession>
    <name>A0A6E8V3W0_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="21">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Postsynaptic cell membrane</location>
            <topology evidence="16">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the ligand-gated ion channel (TC 1.A.9) family. Glutamate-gated chloride channel (TC 1.A.9.4) subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040240679.1">
        <property type="nucleotide sequence ID" value="XM_040384745.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8V3W0"/>
    <dbReference type="EnsemblMetazoa" id="ACON001434-RC">
        <property type="protein sequence ID" value="ACON001434-PC"/>
        <property type="gene ID" value="ACON001434"/>
    </dbReference>
    <dbReference type="GeneID" id="120961090"/>
    <dbReference type="CTD" id="42350"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_014247"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001434"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041837"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008068">
        <property type="term" value="F:extracellularly glutamate-gated chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18993">
        <property type="entry name" value="LGIC_ECD_GluCl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19062">
        <property type="entry name" value="LGIC_TM_GluCl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000024">
        <property type="entry name" value="Glutamate-gated chloride channel alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000022">
        <property type="entry name" value="glutamate-gated chloride channel isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006028">
        <property type="entry name" value="GABAA/Glycine_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044721">
        <property type="entry name" value="GluCl_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00860">
        <property type="entry name" value="LIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00253">
        <property type="entry name" value="GABAARECEPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0868">Chloride</keyword>
    <keyword evidence="11" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15 18" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 18" id="KW-0406">Ion transport</keyword>
    <keyword evidence="14" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="8 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0770">Synapse</keyword>
    <keyword evidence="3 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 18" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="244"/>
            <end position="268"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="18">
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            <begin position="309"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="431"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="19">
        <location>
            <begin position="34"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="20">
        <location>
            <begin position="251"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00061654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001434-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="456" mass="52359" checksum="1C76CFD2D5775D20" modified="2020-08-12" version="1">MASGHFFWAIFYFACLCSASLANNAKVNFREKEKKILDQILGAGKYDARIRPSGINGTDGPAVVRVNIFVRSISKIDDVTMEYSVQLTFREQWLDERLKFDDIGGRLKYLTLTEANRVWMPDLFFSNEKEGHFHNIIMPNVYIRIFPYGSVLYSIRISLTLACPMNLKLYPLDRQVCSLRMASYGWTTADLVFLWKEGDPVQVVKNLHLPRFTLEKFLTDYCNSKTNTGEYSCLKVDLLFKREFSYYLIQIYIPCCMLVIVSWVSFWLDQGAVPARVSLGVTTLLTMATQTSGINASLPPVSYTKAIDVWTGVCLTFVFGALLEFALVNYASRSADRAADMHRENMKKKRREMEQASLDAASDLLDTDSNATFAMKPLVRHPGDPLALEKLRQCEVHMQAPKRPNCCRSWLSKFPTRQCSRSKRIDVISRITFPLVFALFNLVYWSTYLFREEEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V3Y1</accession>
    <name>A0A6E8V3Y1_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="11">SAP domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8V3Y1"/>
    <dbReference type="EnsemblMetazoa" id="ACON001472-RB">
        <property type="protein sequence ID" value="ACON001472-PB"/>
        <property type="gene ID" value="ACON001472"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006075"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001472"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039024"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.30">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043451">
        <property type="entry name" value="Myocardin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004018">
        <property type="entry name" value="RPEL_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003034">
        <property type="entry name" value="SAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036361">
        <property type="entry name" value="SAP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22793:SF12">
        <property type="entry name" value="MYOCARDIN-RELATED TRANSCRIPTION FACTOR, ISOFORM H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22793">
        <property type="entry name" value="MYOCARDIN-RELATED TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02755">
        <property type="entry name" value="RPEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02037">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00707">
        <property type="entry name" value="RPEL"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00513">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68906">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RPEL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50800">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
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        <location>
            <begin position="101"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="repeat" description="RPEL" evidence="7">
        <location>
            <begin position="147"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="SAP" evidence="10">
        <location>
            <begin position="586"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="340"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
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            <end position="470"/>
        </location>
    </feature>
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            <begin position="491"/>
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    </feature>
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            <begin position="695"/>
            <end position="720"/>
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    </feature>
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            <end position="767"/>
        </location>
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            <begin position="1048"/>
            <end position="1091"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
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            <begin position="1105"/>
            <end position="1127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1139"/>
            <end position="1183"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="645"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="340"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="394"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="416"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="446"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="456"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="495"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="527"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="553"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="699"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="754"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1110"/>
            <end position="1126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1150"/>
            <end position="1170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001472-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1278" mass="134831" checksum="81845A2B990ADED8" modified="2020-08-12" version="1">MDEVVVDMDPNMTNRLQQHTVVLDEGAQPGTSYSRVSPPKAVIDSSPLQSAMDKNKQSLIVKLSMRRPYDQLVQQGIMPSLKTSPAIYEQRRQLERAKTGDLLKAKIKKRPDRLELEQRHILEQCDSHVDPSLAEKRRMLEKALLVDHLNSKISHRPGPLDLIGKNILHVEEPIERMVKEGLVDYASTLDESNASVATPTTVGEGEDSLSSEGESLHSVTVAVVNQPGVPATTILQHQIQLQPFPAATPTAATSLQSQPQTLLTAQPEFFTLKPTIPIVVNDGTVLAAIATPTVMAATTAVATIAAVTSSGNIATIAEIPMEQGGGGGAVAATTSSSSSSSTIITSSSNTPSITTSTLRPILPTPSANNNLSGDLATVRPTLTMTPIPSDLKLISSSSSSSNGSGIAGSNGGKEKLAKKKSKTKSITKAKPIKFHEYKGPPNAHRGSSSSSSSSAGHGGGGSGGGGSDNNYQLIMQQQYLLEYLEEMCKKPAGVSSPSSSREAPTTTNTDAEELAGSDQTPIDSPLGPGGSSSGVGGGAGSGSGPGRSKNSTTGGGGTGSGSSVGMGTSTTLPTTFAAMDIALDTLNRLKVSQLKKYCKQYNLAVSGTKSNLIERLKPYIKSIEPCAGGVPSTVGGATAGGTAEAEIEDSLLAEQQKRIAELQLQLKKSQEELEQFRSLCNNPFAASDQPMVINAAVTIPPPPPPPPSSSSSSSTQQPQLASVLVGSPYSITTGGGGDSCDAPCTADGDASAGGTVSANQTPDRVSVPDTTVGAALEGSFFLEGLTPADSTTVDYGHDMHEMLPPELTSTLAPIERSPTHRPTLDKVLGQVQPTISGLTMKTEPTDELRNGGAGGGMSSVVGAAGGNGNAGGVRAAVGDMHDLSLSMKSVNFEAVNGDTGDASMQQDTIMLNDFEDIDLVDFHMHSLDSDGPFPMITNHDPDPAASRTAAHAPPAAATMHDAIDAMFCNGDGDPNGKLLATNSFATNQPTGSQQQQHVLHEHAPPHGFLNNNDLVSGNSLRIMLHMDLMDQNEQQANIATVTGTKQLPFGSLHHQQPQHHHLQHNNQQQQQQHHHTSHLHHSDRNNNSRYDANQTVSLGFGVQQDTQPVASTSASSSSSSTSSSPSNCFRLGLGTSLSSKHANKHHLNLHQQQQQHQHQQQQHQQQQQQQHHPHHHQQHHQSLLEQLTPPSAAAGSDGSNSTLIGDTIDSFQDNAMDFESLLTNDAADSVLNANNCHRMMLDCAQDKPMYSFLPDPSILDYFSDDCNGHDFEIKHLEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V3Y4</accession>
    <name>A0A6E8V3Y4_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="7">LIM zinc-binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8V3Y4"/>
    <dbReference type="EnsemblMetazoa" id="ACON001480-RD">
        <property type="protein sequence ID" value="ACON001480-PD"/>
        <property type="gene ID" value="ACON001480"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001629"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001480"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032806"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001725">
        <property type="term" value="C:stress fiber"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032034">
        <property type="term" value="F:myosin II head/neck binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051496">
        <property type="term" value="P:positive regulation of stress fiber assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051893">
        <property type="term" value="P:regulation of focal adhesion assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08368">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15551:SF3">
        <property type="entry name" value="LIM AND CALPONIN HOMOLOGY DOMAINS-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15551">
        <property type="entry name" value="LIM DOMAIN ONLY 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0440">LIM domain</keyword>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="6">
        <location>
            <begin position="660"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="84"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="129"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="183"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="273"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="472"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="541"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="601"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="153"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="191"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="299"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="337"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="475"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="623"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="634"/>
            <end position="643"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001480-PD"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="731" mass="81622" checksum="3461EB658537C74B" modified="2020-08-12" version="1">MIVFVAWNELLDNALDVAFEEMDSELNESGTVEGALSQREREPSVAQPAETDIVPLSVIPLATDYMPASEEQQQQLVLMALPQSSLTPPKEKPPPPPVEEEPTEQELLQSLTTRGAIITSWMEKGAAMHTHANGNGHHGDDPNANGIGMMMHNSDHRQQQYEDEQWRSLESYEVVQQVVHQQQQQLQHQQQEQEKEQERDKDQHEMMVYSNRIEPALAHHLQQQQQQQLYDPGEHEEIVSVERELLQIEQEELQRRREKQMFYEKLTKADAAAAAAGTRTVTDPAQPLPPQHHHHHQPQHSNQLQQQSQPVQQSPLVQSVRQAPPQQQQQQQPYYYPHHHSHQPSHLHHNLLHHHRASMPAIDSRSLQNVNSYASYDAPFLDEVVNSGYEMGSLHRESMPNLSNVAASSSHPSSLSSYGEEFSANSAASFDGSCWPPAVDHHRGPTGHPGAALFDKRPAVAPRPAMNGSLPPGAAVGGYHGGGGGGQRVSNSMFEQHPKDNKQNTHPSAMVVNRGQGGSSSSGGTNHMPYGQHWLVQEAEQRRIEQHQKSSTNNAKRPLPKFVIDAITQRVQKLNAASPENSDRSNLSEDSDLAMINSMKNHHHHHASMPPPPSSHHHHAHLPHQQQQQQQQPPALPALPPPQQKYSNTSDKVLSVSGKKECSHCRIELGKGAAMAIESLGLFYHIECFKCCVCHVKLGDGTNGIDVRVRKYRLHCQNCFSSEDGVKFSCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V3Z5</accession>
    <name>A0A6E8V3Z5_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Regucalcin</fullName>
            <ecNumber evidence="7">3.1.1.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Gluconolactonase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-glucono-1,5-lactone + H2O = D-gluconate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10440"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16217"/>
            <dbReference type="ChEBI" id="CHEBI:18391"/>
            <dbReference type="EC" id="3.1.1.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="15">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the SMP-30/CGR1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.17" evidence="7"/>
    <dbReference type="RefSeq" id="XP_040226264.2">
        <property type="nucleotide sequence ID" value="XM_040370330.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8V3Z5"/>
    <dbReference type="EnsemblMetazoa" id="ACON001501-RB">
        <property type="protein sequence ID" value="ACON001501-PB"/>
        <property type="gene ID" value="ACON001501"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON001501-RC">
        <property type="protein sequence ID" value="ACON001501-PC"/>
        <property type="gene ID" value="ACON001501"/>
    </dbReference>
    <dbReference type="GeneID" id="120951552"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_014328"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001501"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033866"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004341">
        <property type="term" value="F:gluconolactonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019853">
        <property type="term" value="P:L-ascorbic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000027">
        <property type="entry name" value="Regucalcin homologue"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013658">
        <property type="entry name" value="SGL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005511">
        <property type="entry name" value="SMP-30"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10907:SF66">
        <property type="entry name" value="MIP34848P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10907">
        <property type="entry name" value="REGUCALCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08450">
        <property type="entry name" value="SGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01790">
        <property type="entry name" value="SMP30FAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63829">
        <property type="entry name" value="Calcium-dependent phosphotriesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="SMP-30/Gluconolactonase/LRE-like region" evidence="16">
        <location>
            <begin position="20"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="14">
        <location>
            <position position="221"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00008853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00016808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00032464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR605511-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR605511-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF08450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001501-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="310" mass="33795" checksum="3D724CA344CD5DA6" modified="2020-08-12" version="1">MASSDSSYQVVKLPSPRTKLGEGPVWDIDSQSLYYVDINTPAVLRYDYGENRTYSAKLAGANSISFIALVTGQPEHFVVGENNRVTLIRWDGRSEAASHVRVLADLGPSQSHVRFNDGKIDPAGRLYAGTMQLESLGDLFAQKEGQLFRYTNGTMVVQKQNVSISNGLTWDEPFNPLRMYYIDSAALDVKAFDVDANGDLKNETVFYDLRVNGAHPGYVPDGMTSDADGNLYVATWGGSKVMKIDKGSQKLVLEIKIPAEQVTSVAFGGPQMDELFVTTSSNGDKPAPAGELFKVTGLGVKGKPMHKMVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V8A2</accession>
    <name>A0A6E8V8A2_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phosphodiesterase</fullName>
            <ecNumber evidence="9">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. Vital for female fertility. Required for learning/memory.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="9">Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the cyclic nucleotide phosphodiesterase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="9"/>
    <dbReference type="RefSeq" id="XP_040238404.1">
        <property type="nucleotide sequence ID" value="XM_040382470.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8V8A2"/>
    <dbReference type="EnsemblMetazoa" id="ACON000236-RC">
        <property type="protein sequence ID" value="ACON000236-PC"/>
        <property type="gene ID" value="ACON000236"/>
    </dbReference>
    <dbReference type="GeneID" id="120959054"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011468"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000236"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040689"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004114">
        <property type="term" value="F:3',5'-cyclic-nucleotide phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007615">
        <property type="term" value="P:anesthesia-resistant memory"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048675">
        <property type="term" value="P:axon extension"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007623">
        <property type="term" value="P:circadian rhythm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001661">
        <property type="term" value="P:conditioned taste aversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007619">
        <property type="term" value="P:courtship behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046958">
        <property type="term" value="P:nonassociative learning"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008355">
        <property type="term" value="P:olfactory learning"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007614">
        <property type="term" value="P:short-term memory"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040040">
        <property type="term" value="P:thermosensory behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1300.10:FF:000001">
        <property type="entry name" value="Phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1300.10">
        <property type="entry name" value="3'5'-cyclic nucleotide phosphodiesterase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040844">
        <property type="entry name" value="PDE4_UCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023088">
        <property type="entry name" value="PDEase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002073">
        <property type="entry name" value="PDEase_catalytic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036971">
        <property type="entry name" value="PDEase_catalytic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023174">
        <property type="entry name" value="PDEase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11347">
        <property type="entry name" value="CYCLIC NUCLEOTIDE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18100">
        <property type="entry name" value="PDE4_UCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00233">
        <property type="entry name" value="PDEase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00387">
        <property type="entry name" value="PDIESTERASE1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00126">
        <property type="entry name" value="PDEASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51845">
        <property type="entry name" value="PDEASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0114">cAMP</keyword>
    <keyword evidence="3 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDEase" evidence="11">
        <location>
            <begin position="417"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="11"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="118"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="176"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="247"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="756"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="11"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="118"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="142"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="176"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="793"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="829"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="493"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="493"/>
            <end position="497"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="497"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="533"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="534"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="534"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="534"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="666"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="666"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="717"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00053071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000236-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="851" mass="90979" checksum="D71AE0994FF2F7A5" modified="2023-06-28" version="2">MFRNKYFFSSSGGGAGGTGSGSGGNKRRGSTALPHCFMDNAEGSGSGPPSGVMSVVGGYTSRRSPLLLPSGDGGPGGPGALLSAALPQRRESFLYRSDSDFEISPKVSSVSLLAARCSANNSGSGTSTSNVASTGSGTTLTSGGGGGTGGGSRDGAFHWKETSTLEEPAVLSSIASSPSSSATTVVGAASSTVVTSSTSERTTMMSGSGGGGSSGHHDDLIVTPFAQILASLRTVRSNLYALSSALPEESELATRRSSAGAESAETDPGSAAGDDSQPDKDGDLRQLTTETIDELEWCLEQLETVQTHRSVSDMASLKFKRLLNKELSHLSESSKSGNQISEYICTTFLDKQQEPDVPSAPLVGSALIGRPTGAAGRMSEISGFKRPLQHTNSFSGAAGAGVTSLADRLPPYGVPTATAADEAELGGLLGQIDQWGVDIFRIGTLSGSRPLTAVAYTVFQDRNIPRTLTIPPTVLLAFVSTVEDHYVADNPFHNSLHAADVTQSTHALLHSPALDGVFTPLEVCAALVAACIHDVDHPGLTNQFLINSSSELALMYNDESVLENHHLAVAFKLMQNDDCNILRNLPKKQRATFRKMVIDMVLSTDMSKHMTLLADLKTMVETKKVAISGNQNPGESRSSGSGVLLRLLDSYTDRIQVLQNLVHCADLSNPTKPLPLYRRWVRRLMEEFFRQGDRERAAGMDVSPMCDRRSATVESSQVGFIDYIVHPLWEAWAELVRPDAQDILDRLEENRAYYQALIPPPSPPSHEKDEEEEEEAEEAAKREAKQQQQQQQERAEQEAGSKRNEQQEPMPIDPQALAARLRFQMTLAENDEEEDDLEPDGGAGNQAQEHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V8B2</accession>
    <name>A0A6E8V8B2_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Discs large MAGUK scaffold protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the MAGUK family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8V8B2"/>
    <dbReference type="EnsemblMetazoa" id="ACON000255-RA">
        <property type="protein sequence ID" value="ACON000255-PA"/>
        <property type="gene ID" value="ACON000255"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030881"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043296">
        <property type="term" value="C:apical junction complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031594">
        <property type="term" value="C:neuromuscular junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098839">
        <property type="term" value="C:postsynaptic density membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043297">
        <property type="term" value="P:apical junction assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009792">
        <property type="term" value="P:embryo development ending in birth or egg hatching"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045197">
        <property type="term" value="P:establishment or maintenance of epithelial cell apical/basal polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043113">
        <property type="term" value="P:receptor clustering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097120">
        <property type="term" value="P:receptor localization to synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099072">
        <property type="term" value="P:regulation of postsynaptic membrane neurotransmitter receptor levels"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00071">
        <property type="entry name" value="GMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06723">
        <property type="entry name" value="PDZ1_Dlg1-2-4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06724">
        <property type="entry name" value="PDZ2_Dlg1-2-4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06795">
        <property type="entry name" value="PDZ3_Dlg1-2-4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11861">
        <property type="entry name" value="SH3_DLG-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001402">
        <property type="entry name" value="Discs, large homolog 3 (Drosophila)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000001">
        <property type="entry name" value="Disks large homolog 1 isoform 2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Disks large homolog 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000058">
        <property type="entry name" value="Disks large homolog 1 isoform X1"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="2.30.42.10:FF:000048">
        <property type="entry name" value="disks large homolog 1 isoform X1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Disks large homolog 4 isoform 2"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.63.10">
        <property type="entry name" value="Guanylate Kinase phosphate binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016313">
        <property type="entry name" value="DLG1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008144">
        <property type="entry name" value="Guanylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020590">
        <property type="entry name" value="Guanylate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
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    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
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        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050614">
        <property type="entry name" value="Synaptic_Scaffolding_LAP-MAGUK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23119">
        <property type="entry name" value="DISCS LARGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23119:SF51">
        <property type="entry name" value="DISKS LARGE 1 TUMOR SUPPRESSOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PDZ"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MAGUK_DLGH"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PDZ"/>
        <property type="match status" value="3"/>
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        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00856">
        <property type="entry name" value="GUANYLATE_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50052">
        <property type="entry name" value="GUANYLATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0965">Cell junction</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 12" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="PDZ" evidence="16">
        <location>
            <begin position="115"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="16">
        <location>
            <begin position="212"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="16">
        <location>
            <begin position="403"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="14">
        <location>
            <begin position="514"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate kinase-like" evidence="15">
        <location>
            <begin position="690"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="37"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="578"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="626"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="60"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000255-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="19">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="880" mass="97287" checksum="5855043983DD3B64" modified="2023-06-28" version="1">MIDWVSIVRHSRHRISSYVSPRRRTDKPSDRYTVCTDSIPLPHHAAKSKVSANRIKQQEQEQQQQQLQQQQQQQQQAPPQLQLQQEIEKQPIIELPSPRLTTTKLNGDSPWEYDEITLIRGSTGLGFSIAGGTDNPHINLDASIYITKVIPGGAAHVDGRLQVNDCIVAVNEVRVVNVTHGEAVDALKQAGDRVTLHIRRKRPPAQAPKLEEIELLKGSKGLGFSIAGGIGNQHIPGDNGIYVTKIMEGGAAHIDGRLAVGDKLIAVRTPDGGERNLENVVHEEAVATLKAITNRATLIVQKTNILQALANSISNLNSLSTNAMNSVGVSMVDHASPDAGGGGGEAGGGDTEMVASTARSHSPAFGLENSSSRYASSNVLETGMPIGTPRAVSSEDITRVPRTIVIRKGASGLGFNIVGGEDGQGIFVSYVLAGGAADLGGELKRGDQLLSVNGISLANASHEDAAQALKNAGGTVTLVVQYRPEDYNRFEQRIQELKQAVAAGTGTLLRTTQKRTLYVRALFDYDPNRDDGLPTRGLQFRHGDILHVTNASDDEWWQARRVIGEDEEESIGIVPSKRRWERKQRARDRSVKFQGHAGANNNNNNLEKVSTLDRKKKNFSFSRRFPFMKSKDDKNEDGSDQEPNGNLSDPNHHDPDQPFMLCYTQEDANAEAEENILSYEPVQRLQISYTRPVIILGPLKDRINDDLISEYPNKFGSCVPHTTRPKRDYEVDGRDYHFIASREQMEKDIQNHLFIEAGQYNDNLYGTSVASVREVAEKGKHCILDVSGNAIKRLQVAQLYPIAIFIKPMSVESIMEMNRRMTEEQAKKTYERTLKMEHEFGEYFTAIVQGDTIEEIYSKVKNVIWSQGGPTIWVPSKESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V8C9</accession>
    <name>A0A6E8V8C9_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phosphodiesterase</fullName>
            <ecNumber evidence="9">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. Vital for female fertility. Required for learning/memory.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="9">Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the cyclic nucleotide phosphodiesterase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="9"/>
    <dbReference type="RefSeq" id="XP_040238318.2">
        <property type="nucleotide sequence ID" value="XM_040382384.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8V8C9"/>
    <dbReference type="EnsemblMetazoa" id="ACON000236-RA">
        <property type="protein sequence ID" value="ACON000236-PA"/>
        <property type="gene ID" value="ACON000236"/>
    </dbReference>
    <dbReference type="GeneID" id="120959054"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011468"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000236"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040689"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004114">
        <property type="term" value="F:3',5'-cyclic-nucleotide phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007615">
        <property type="term" value="P:anesthesia-resistant memory"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048675">
        <property type="term" value="P:axon extension"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007623">
        <property type="term" value="P:circadian rhythm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001661">
        <property type="term" value="P:conditioned taste aversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007619">
        <property type="term" value="P:courtship behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046958">
        <property type="term" value="P:nonassociative learning"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008355">
        <property type="term" value="P:olfactory learning"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007614">
        <property type="term" value="P:short-term memory"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040040">
        <property type="term" value="P:thermosensory behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1300.10:FF:000001">
        <property type="entry name" value="Phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1300.10">
        <property type="entry name" value="3'5'-cyclic nucleotide phosphodiesterase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040844">
        <property type="entry name" value="PDE4_UCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023088">
        <property type="entry name" value="PDEase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002073">
        <property type="entry name" value="PDEase_catalytic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036971">
        <property type="entry name" value="PDEase_catalytic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023174">
        <property type="entry name" value="PDEase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11347">
        <property type="entry name" value="CYCLIC NUCLEOTIDE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18100">
        <property type="entry name" value="PDE4_UCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00233">
        <property type="entry name" value="PDEase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00387">
        <property type="entry name" value="PDIESTERASE1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00126">
        <property type="entry name" value="PDEASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51845">
        <property type="entry name" value="PDEASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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        <ligand>
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        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
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    <sequence length="929" mass="99587" checksum="7B584F28F7F94621" modified="2020-08-12" version="1">MEPSAPEQQQQQQRATTAVGYGQRTKDSATFEYRTVRIQQNCQQQVIYSSSSSSTSASSSSTVHRSTRAGESALGSSAAASRLMLTMGRPNHQLPPGLSLFGCAGGANYQRCKLFMDNAEGSGSGPPSGVMSVVGGYTSRRSPLLLPSGDGGPGGPGALLSAALPQRRESFLYRSDSDFEISPKVSSVSLLAARCSANNSGSGTSTSNVASTGSGTTLTSGGGGGTGGGSRDGAFHWKETSTLEEPAVLSSIASSPSSSATTVVGAASSTVVTSSTSERTTMMSGSGGGGSSGHHDDLIVTPFAQILASLRTVRSNLYALSSALPEESELATRRSSAGAESAETDPGSAAGDDSQPDKDGDLRQLTTETIDELEWCLEQLETVQTHRSVSDMASLKFKRLLNKELSHLSESSKSGNQISEYICTTFLDKQQEPDVPSAPLVGSALIGRPTGAAGRMSEISGFKRPLQHTNSFSGAAGAGVTSLADRLPPYGVPTATAADEAELGGLLGQIDQWGVDIFRIGTLSGSRPLTAVAYTVFQDRNIPRTLTIPPTVLLAFVSTVEDHYVADNPFHNSLHAADVTQSTHALLHSPALDGVFTPLEVCAALVAACIHDVDHPGLTNQFLINSSSELALMYNDESVLENHHLAVAFKLMQNDDCNILRNLPKKQRATFRKMVIDMVLSTDMSKHMTLLADLKTMVETKKVAISGNQNPGESRSSGSGVLLRLLDSYTDRIQVLQNLVHCADLSNPTKPLPLYRRWVRRLMEEFFRQGDRERAAGMDVSPMCDRRSATVESSQVGFIDYIVHPLWEAWAELVRPDAQDILDRLEENRAYYQALIPPPSPPSHEKDEEEEEEAEEAAKREAKQQQQQQQERAEQEAGSKRNEQQEPMPIDPQALAARLRFQMTLAENDEEEDDLEPDGGAGNQAQEHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V967</accession>
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        <name type="scientific">Anopheles coluzzii</name>
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            <taxon>Anophelinae</taxon>
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        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
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    <reference evidence="5" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
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                <consortium name="EnsemblMetazoa"/>
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        <scope>IDENTIFICATION</scope>
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            <end position="23"/>
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    </evidence>
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        <source>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V970</accession>
    <name>A0A6E8V970_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Protein kinase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
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                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. Lammer subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8V970"/>
    <dbReference type="EnsemblMetazoa" id="ACON000847-RB">
        <property type="protein sequence ID" value="ACON000847-PB"/>
        <property type="gene ID" value="ACON000847"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000199"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000847"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039891"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043484">
        <property type="term" value="P:regulation of RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14134">
        <property type="entry name" value="PKc_CLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000145">
        <property type="entry name" value="Dual specificity protein kinase CLK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051175">
        <property type="entry name" value="CLK_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45646">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE DOA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45646:SF11">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE DOA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="202"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="18"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="528"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="47"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="85"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="543"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000847-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="553" mass="62467" checksum="A44C042DD8C22751" modified="2020-08-12" version="1">MQTQPAEYKQAYCTVHPKVTHQHQQQQQQQYEAGRILTAGGEQAPQNGTNTNTPKKPTDPGGPVAPAAPTTTATSDGRSKRSKSPPRSCSGGKPGPELTVNGGGVGGATGPVTSTVPSTIRGSGPNGELTLKDVPVSGISFQEIFKPDHEGPPTTQVIIYKPKSGRGQPTAKVIVTKKQPFIRDDADGHLIYQIGDILHNRYKILATLGEGTFGRVVKVKDLERNHVMALKIIKNVEKYRDAAELEIGALEKIMQLDPNLEHLCVKMLDWFDYHGHTCIAFEMLGLSVFDFLKDNNYEPYPIEHVRHISYQLCYAVKFLHESRLTHTDLKPENILFVDSEYTTTTVPRKNREVRRVNCTDIRLIDFGSATFDDEHHSTIVSTRHYRAPEVILELGWSQPCDVWSIGCIMFELYQGVTLFPTHDNREHLAMMERILGTIPYRMARQTRSKYFRYGKLDWDEKSSTGRYVRDNCKPLHRCVITDKPDHLQLFDLIRKMLEYEPSKRITLDKALRHPFFAKLPAHQRLHEKCNDSPVGAHSSTANAERERSHSLSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VA37</accession>
    <name>A0A6E8VA37_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Microtubule-associated protein Jupiter</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds to all microtubule populations.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the MAP Jupiter family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VA37"/>
    <dbReference type="EnsemblMetazoa" id="ACON001372-RA">
        <property type="protein sequence ID" value="ACON001372-PA"/>
        <property type="gene ID" value="ACON001372"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004269"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001372"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042882"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033335">
        <property type="entry name" value="JUPITER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34930">
        <property type="entry name" value="GEO05313P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34930:SF2">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEIN JUPITER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17054">
        <property type="entry name" value="JUPITER"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="38"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="60"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="128"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="172"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001372-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="209" mass="21454" checksum="A76CA65534A82480" modified="2020-08-12" version="1">MTSTNFTVGFGENSKASSKVLKPPGGGSSDLFGADQLGPNTPRTMSKNRMQSNIFAAPSDAHKNGSETPRRNGQGQDSHNRLFGEVNRPFTPAKNHMKSNLPIGNPGDSTDGAKPHQNGKANGVAHQNGNGTANGHHTNGNGHTNGNGHISSASSDNGSSHNGNPVTGEGYSKSNGAAEINTTVPSLNGGGHVINKNRIPPGGFSSGLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VA38</accession>
    <name>A0A6E8VA38_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Myosin light chain alkali</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Myosin is a hexamer of 2 heavy chains and 4 light chains.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040224914.2">
        <property type="nucleotide sequence ID" value="XM_040368980.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VA38"/>
    <dbReference type="EnsemblMetazoa" id="ACON001569-RB">
        <property type="protein sequence ID" value="ACON001569-PB"/>
        <property type="gene ID" value="ACON001569"/>
    </dbReference>
    <dbReference type="GeneID" id="120950725"/>
    <dbReference type="CTD" id="23209"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005061"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001569"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041204"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005859">
        <property type="term" value="C:muscle myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000267">
        <property type="entry name" value="Myosin light chain alkali"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF33">
        <property type="entry name" value="MYOSIN LIGHT CHAIN ALKALI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="4" id="KW-0518">Myosin</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="7">
        <location>
            <begin position="8"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="7">
        <location>
            <begin position="82"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001569-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="154" mass="17542" checksum="DD025F3845D5E6A1" modified="2020-08-12" version="1">MANDLKDVEIEKAQFVFSVYDWEGSGQMDAMDLGNALRALNLNPTIELIGKMGGTQKRGEKKIKFEEFLPIFSQVKKEKEQGCFEDFLECLKLYDKNEDGTMLLAELTHSLTALGERLDDVELDNVMKDCMDPEDDDGNIPYAPFLQRLCDHKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VBT6</accession>
    <name>A0A6E8VBT6_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Tumor necrosis factor alpha-induced protein 8-like protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VBT6"/>
    <dbReference type="EnsemblMetazoa" id="ACON002997-RA">
        <property type="protein sequence ID" value="ACON002997-PA"/>
        <property type="gene ID" value="ACON002997"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON002997-RB">
        <property type="protein sequence ID" value="ACON002997-PB"/>
        <property type="gene ID" value="ACON002997"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_008051"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002997"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_036534"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1440.160:FF:000001">
        <property type="entry name" value="Tumor necrosis factor alpha-induced protein 8-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.160">
        <property type="entry name" value="Tumor necrosis factor alpha-induced protein 8-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008477">
        <property type="entry name" value="TNFAIP8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038355">
        <property type="entry name" value="TNFAIP8_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12757:SF1">
        <property type="entry name" value="PROTEIN SALIVARY GLANDS MARRED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12757">
        <property type="entry name" value="TUMOR NECROSIS FACTOR INDUCED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05527">
        <property type="entry name" value="TNFAIP8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002997-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="188" mass="21464" checksum="5FCCD356DF12C735" modified="2020-08-12" version="1">MTDNAFKAKDIGLRAQKKILSRMASKNVAKVFIDGTTASLLDNVYRLVKVHTGSKKDAERLVKNIIKIVIKIAVLHRNGQLNADELRQADRFRTKFQTLQMAILSFYEVDYSFDLNYLQKSLADCRSLLRSCVVRHLTDKSLGRIDEVFDTFADSALLETAFRQDSPYREIMDKIVVDLNKAMENGDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VBV6</accession>
    <name>A0A6E8VBV6_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">REST corepressor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the CoREST family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VBV6"/>
    <dbReference type="EnsemblMetazoa" id="ACON002488-RB">
        <property type="protein sequence ID" value="ACON002488-PB"/>
        <property type="gene ID" value="ACON002488"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011777"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002488"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031644"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000118">
        <property type="term" value="C:histone deacetylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000033">
        <property type="entry name" value="REST corepressor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1240.50:FF:000002">
        <property type="entry name" value="REST corepressor isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1240.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000949">
        <property type="entry name" value="ELM2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051066">
        <property type="entry name" value="Trans_reg/Corepressor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16089:SF28">
        <property type="entry name" value="REST COREPRESSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16089">
        <property type="entry name" value="REST COREPRESSOR COREST PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01448">
        <property type="entry name" value="ELM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01189">
        <property type="entry name" value="ELM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51156">
        <property type="entry name" value="ELM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="ELM2" evidence="9">
        <location>
            <begin position="57"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="10">
        <location>
            <begin position="143"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="22"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="197"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="32"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="198"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002488-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="317" mass="35552" checksum="B89C0DAA2D77198D" modified="2020-08-12" version="1">MGSDREAQALTVMRVPGWSALPARPVALPTPARTTKTVTRKNGNSSKATKTKSEYEEKIRVGRDYQAVCPELVAPNERKPEQLNDRALLVWSPTKEIPDNKLEEYITVAKEKYGYNGEQALGMLFWHKHDLERAVLDLANFTPFPDEWSAEDRVLFEQAFQFHGKSFHRIRQMLPDKSIASLVKFYYSWKKTRSRTSVMDRQEKMKKNDSSENGSDHGSNEESDNEDKIDLVDDDNNRVTATANGKSKEPTDSVLISSNNMIDDDTSINNMESVSVNSLSAAITLDSSLSMDVDEVTITPNIKGNDAADASNTVIIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VBW7</accession>
    <name>A0A6E8VBW7_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="12">FA_desaturase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the fatty acid desaturase type 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VBW7"/>
    <dbReference type="EnsemblMetazoa" id="ACON003051-RA">
        <property type="protein sequence ID" value="ACON003051-PA"/>
        <property type="gene ID" value="ACON003051"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010073"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003051"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_029706"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004768">
        <property type="term" value="F:stearoyl-CoA 9-desaturase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006636">
        <property type="term" value="P:unsaturated fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015876">
        <property type="entry name" value="Acyl-CoA_DS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11351">
        <property type="entry name" value="ACYL-COA DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11351:SF26">
        <property type="entry name" value="FATTY ACID DESATURASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="42"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="66"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003051-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="207" mass="23744" checksum="165EEF4295885F28" modified="2020-08-12" version="1">DFMHAQVFANIRKLSPRQEQLLAAVDMSDLEADGIVMFQRRFYWAMYLVLFVLLPINAPLEYWGDTVQAAIFVAFSLRYLVVLNVAWLINSAHFVWGLDKNHKQSDSNMVFLVTKSYWPQYHYLLPFDYQSGEFGNYGSGCTTAFIRIFAAIGQATKLQTITTEAVKRGLTMAVDSGRPIVECLKQAGAEEMCNLKREHYLETERLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VC52</accession>
    <name>A0A6E8VC52_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="3">N-myc downstream regulated</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NDRG family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VC52"/>
    <dbReference type="EnsemblMetazoa" id="ACON003238-RD">
        <property type="protein sequence ID" value="ACON003238-PD"/>
        <property type="gene ID" value="ACON003238"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004640"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003238"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033801"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004142">
        <property type="entry name" value="NDRG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11034">
        <property type="entry name" value="N-MYC DOWNSTREAM REGULATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03096">
        <property type="entry name" value="Ndr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="298"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="301"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003238-PD"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="347" mass="38188" checksum="F6B4DD1701D27013" modified="2020-08-12" version="1">MGRRRYAVNTEKSGEIIVTVQGDLSQQEKRAVFLTVHDLGTNHSSFEEFVNSACMIEIKERSCFIHIDVPGHADNAPNLADSFQFPSLQLLGEELVTVLDFLHVKYVIGVGEGAGANVLARFGLAHPSRCLGLILINVTGSAASVLDVFKTKFISWKGDEVGQSAEDFLLYHKFGYQLVGDNPDKEKIVSEFQSRLHSSLNSKNLKQYVKAFMSRKDLPLKNCKVDCLLITGIMSPYASVVEKLYKDLNKEKVTLLKVERAGDVLADAPAKVAQSILLFCKGQGLLTSVAMPGVDRNRAFSTSSSGSTEGSTGARRLSRGMSMEDYDKPNIRRLSVTINEQLPPIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VC73</accession>
    <name>A0A6E8VC73_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Raw</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_040240714.2">
        <property type="nucleotide sequence ID" value="XM_040384780.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040240715.2">
        <property type="nucleotide sequence ID" value="XM_040384781.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VC73"/>
    <dbReference type="EnsemblMetazoa" id="ACON002736-RA">
        <property type="protein sequence ID" value="ACON002736-PA"/>
        <property type="gene ID" value="ACON002736"/>
    </dbReference>
    <dbReference type="GeneID" id="120961110"/>
    <dbReference type="CTD" id="44851"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_003788"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002736"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031294"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.450:FF:000017">
        <property type="entry name" value="Raw, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.450">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039470">
        <property type="entry name" value="Nuc_deoxyri_tr2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36300">
        <property type="entry name" value="RAW, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36300:SF1">
        <property type="entry name" value="RAW, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15891">
        <property type="entry name" value="Nuc_deoxyri_tr2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="463"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="93"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="497"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="519"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002736-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="761" mass="83607" checksum="28D12997BBC97C37" modified="2020-08-12" version="1">MMRPSTLDIKTNYHDGCYTFMRDAYRCWKARAESGEQPYIAELFADLQQSLSRKQVRDILFTMKNVTRAPAMNTTRPSSVQTPVANATLSLSAAAAAPSQQQPNRQHSPSQQRKDRPNRPPSPPGERLTFGEFTAIASCTKCFGRQSSFHAMRARNVDELGSVLANHAGTAKDGEPSPGPEVFLGGSCNPTTWRADVAIPTLDRLGISFYNPQVSHWTPDLIELEHRAKEKAKVLFFVLDSQTRSTAGAIEVAHIAGQNSKYLLLVLLPYSSRQKILNETLLEDEYMDLMSNQLMLRQLVLRRGLPVLDSIPTALEQIKEVLLGNCCPQPPVNLASRLISLRRTFERVAADVTESARINLAQCQQALGSLGYPANVGTMGNIQNILLCYEEVTGLDVQTQLIRRGDAESVLISFEEFCIIDACVSILMLDFYEHNCTSPIKGTNLQQPPVYLTDLQAWNHTNPELSPFENGSLKSPRTFVLPKSGHEPAVNGTTDPSAVAAAASVAGPPSSRSSSGSSELNHTTTTTPLSVDAGPGGEERKLHSPVPLPPAIDEPRAFTFDGDPFGDDGDDGEPLLGRIEPRDVYLGGSCWMKTSWRQRWAFPMLKRHNLSYYVSALHEGFCCGKPEQPVGGDGSAGQQQQQQFDPQSTLRYDAAMLDACRIVIFAITNETRSLAPMTMAAHYIGLGYNVVLCVQMLPSNESAPNGHNTTLSSSAIKDYNRGRAYLIDLAKRHGIPVYEGLEETFDSVISRLRNGTVRYST</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VC83</accession>
    <name>A0A6E8VC83_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Fatty acid hydroxylase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_049461229.1">
        <property type="nucleotide sequence ID" value="XM_049605272.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VC83"/>
    <dbReference type="EnsemblMetazoa" id="ACON002767-RA">
        <property type="protein sequence ID" value="ACON002767-PA"/>
        <property type="gene ID" value="ACON002767"/>
    </dbReference>
    <dbReference type="GeneID" id="125906471"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_013155"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002767"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031270"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006694">
        <property type="entry name" value="Fatty_acid_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050307">
        <property type="entry name" value="Sterol_Desaturase_Related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11863">
        <property type="entry name" value="STEROL DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04116">
        <property type="entry name" value="FA_hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="45"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="202"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acid hydroxylase" evidence="6">
        <location>
            <begin position="144"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002767-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="297" mass="34673" checksum="7ADC62094686E3ED" modified="2020-08-12" version="1">METLLELAANVTLLPVEEFAGPQVSWFERKWNGFLDVIGDDVDTLYMWFLTAYTYGFFWLVGGLFVLMDVTNKPSFLRKFKNQPGANEPLPWAKLKNLVKTVAYNQLVYGLPTSYLSFRVGKLIAASIPDPRILPSPYIIVRDVMVCIVAWEITFYYSHRLLHSSFFYKRIHKKHHEWSAPVAWAAMYAHPFEFIISDLLPVYVGPALMTSHVFTIVIWFTFVMMDTLVDHSGYHLPVLGSSEMHDYHHLKFNQCYGLFGWWDGLHGTDSEFRKKKQYQRHHRIFSLKSARELVPEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VCK0</accession>
    <name>A0A6E8VCK0_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Elongin-B</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Elongin 18 kDa subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">RNA polymerase II transcription factor SIII subunit B</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">SIII p18</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Transcription elongation factor B polypeptide 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex). In embryonic stem cells, the elongin BC complex is recruited by EPOP to Polycomb group (PcG) target genes in order generate genomic region that display both active and repressive chromatin properties, an important feature of pluripotent stem cells.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Heterotrimer of an A (ELOA, ELOA2 or ELOA3P), ELOB and ELOC subunit. The elongin BC complex interacts with EPOP; leading to recruit the elongin BC complex to Polycomb group (PcG) target genes, thereby restricting excessive activity of the PRC2/EED-EZH2 complex. Component of multiple cullin-RING E3 ubiquitin-protein ligase complexes composed of Elongin BC (ELOB and ELOC), a cullin (either CUL2 or CUL5), a catalytic subunit (either RBX1 or RNF7/RBX2), as well as a substrate adapter protein that can be either ASB2, ASB9, ASB11, KLHDC2, KLHDC3, KLHDC10, APPBP2, FEM1A, FEM1B, FEM1C, LRR1, PCMTD1, SOCS1, SOCS2, SOCS5, SPSB1, SPSB3, ELOA, VHL, WSB1 or RAB40C. As part of the Elongin BC E3 ubiquitin ligase complex; interacts with NRBP1. May also interact with DCUN1D1, DCUN1D2, DCUN1D3 and DCUN1D5. May form oligomers as a KLHDC2/KLHDC3-ELOB-ELOC complex; this interaction is autoinhibitory for the E3 ligase complex as the substrate-binding site of KLHDC2/KLHDC3 is blocked in the oligomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the Elongin B family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VCK0"/>
    <dbReference type="EnsemblMetazoa" id="ACON003154-RA">
        <property type="protein sequence ID" value="ACON003154-PA"/>
        <property type="gene ID" value="ACON003154"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000358"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003154"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037230"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070449">
        <property type="term" value="C:elongin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030891">
        <property type="term" value="C:VCB complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01788">
        <property type="entry name" value="Ubl_ElonginB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000108">
        <property type="entry name" value="Elongin-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039049">
        <property type="entry name" value="ELOB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13248:SF4">
        <property type="entry name" value="ELONGIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13248">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR B POLYPEPTIDE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="18">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="86"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="102"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00054216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00060803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00074516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00076690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00080438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00081013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00083653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00093515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003154-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="113" mass="12745" checksum="CE504608C67F72A6" modified="2020-08-12" version="1">MMIRRKKTTIFTDAKETTPVYELKKMIEGILKVPPRDQRLYNKDNMLMDDDKTLQDCGITVVTAKAQCPAQLGLAVRENGDFESLELTPYSLPPDLPDVMKNQDTANGQDQLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VDX3</accession>
    <name>A0A6E8VDX3_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="5">RGS domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VDX3"/>
    <dbReference type="EnsemblMetazoa" id="ACON000232-RA">
        <property type="protein sequence ID" value="ACON000232-PA"/>
        <property type="gene ID" value="ACON000232"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_013732"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000232"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037041"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009968">
        <property type="term" value="P:negative regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008277">
        <property type="term" value="P:regulation of G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04450">
        <property type="entry name" value="DEP_RGS7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00068">
        <property type="entry name" value="GGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08705">
        <property type="entry name" value="RGS_R7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.167.10:FF:000001">
        <property type="entry name" value="Putative regulator of g-protein signaling 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1240.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.260.10">
        <property type="entry name" value="Transducin (heterotrimeric G protein), gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000591">
        <property type="entry name" value="DEP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015898">
        <property type="entry name" value="G-protein_gamma-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036284">
        <property type="entry name" value="GGL_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016137">
        <property type="entry name" value="RGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047016">
        <property type="entry name" value="RGS6/7/9/11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047017">
        <property type="entry name" value="RGS6/7/9/11_DHEX_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040759">
        <property type="entry name" value="RGS_DHEX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034483">
        <property type="entry name" value="RGS_Egl-10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036305">
        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45746">
        <property type="entry name" value="LP21163P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45746:SF5">
        <property type="entry name" value="REGULATOR OF G-PROTEIN SIGNALING 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00610">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00631">
        <property type="entry name" value="G-gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00615">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18148">
        <property type="entry name" value="RGS_DHEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01301">
        <property type="entry name" value="RGSPROTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00049">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01224">
        <property type="entry name" value="G_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00224">
        <property type="entry name" value="GGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00315">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48670">
        <property type="entry name" value="Transducin (heterotrimeric G protein), gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50186">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50132">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0734">Signal transduction inhibitor</keyword>
    <feature type="domain" description="DEP" evidence="4">
        <location>
            <begin position="173"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="RGS" evidence="3">
        <location>
            <begin position="445"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="74"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="123"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="343"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="581"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="705"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="821"/>
            <end position="998"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1084"/>
            <end position="1103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1129"/>
            <end position="1243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="123"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="353"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="585"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="823"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="845"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="882"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="943"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="978"/>
            <end position="997"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1129"/>
            <end position="1148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1214"/>
            <end position="1226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000232-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1254" mass="134944" checksum="F1D3EE7488048D07" modified="2023-06-28" version="2">MAMDPPDVVLSPERESSLVATAGSSSGGGGSKSTTAALLQLQHRAAHPQTPPAAFHLTGGGVGEPALHQQRSALLVQQSSTTTTPPGSSGGGSAAATAGVTQLTGIAVPTALLASAGQLQSVAGGSNSSAVATPSGLTTTGSSGTQPAPPIERLSRPMAFDKMESLVREMQDSENGVPVRSQKLFLTSIPSAFMGYDLIEWLMERLSIEESEALNISNQLCQHGYFFPVSDSKTLMVKDDSSLYRFQTPYYWPWQQKAPDQIEYAIYLAKRSLRNKQRHGLEDYETEALASLHKNLKGKWDFILMQAEEQVRLAKDRKKGDKIVGDSQERAYWRVHRPPPGQFTPLEPCPIPSRDRQGKPRKRTVEDWQREVDILKASLGRNRVKMSQACESLVVYYETFSEYDPMMQPPLPSNPWVTEDVSFWQLNHPVVEVPTEKRVKRWGISIEEVVSDPTGLQEFTHYLKKEYSHENIRFWMAVNDLRRSSHSQISRKVKEIYEEFLEPGAPCEINIDGKTMEKVQLGLKNPSRFAFDAAAEHIYTLLLKKDCYPRFVRSEHYKNLMANAIQPSLKKRFFGFGGGAKKKGSTSTAPNPSMLTQPSTGQSGSGNVNSLSKRRGSDRSLSGSAHELAISGVKDCSKVPHSHSQSNLSDIPYREMNKNLGTTLEVTLEGAVSGACAVCPWDTPVIEKPAAESAVSVSVCPWDSADAKLPTKTDTNSSKSKQHGDHHPPHSTAEISEVSERMKRSCGLRQNTLDGDFHSTKRLAPVVTEQRRASMTMAPRLSDLQFCMRANSTGSGATPPDSTACLANLPSQQQQYLQHQSSVRISQGSLSTSFEEKEEGGSGTVFYQQDGTSGAPTPIGEERAGQAGTVADDVAQQDRGPGDNPDAAEGAAGARDATPQAQPEADVPPVLPAATSTEPLGAATEQVGTQQQPYGGVPAAAVCPTSNPTTTTNTTITTTTTTICTSSAGAGRAMAREANSQRPTNVDTAPESTTGTPRISVIYANTPDDDDEPAEEYELAVGSEPEATVREASVQHGSPAAEPAAASVATQTVDVLLLQGVDAAQDLSPLTEAEDGFYGEALRRQHHQHQQHHPSEYDIMMDGGESGLLPGCVAPAPTPAPSIAPLAEVELDPPDDEPDEEEQEDGGGLEEGTGRLDGEPTGAAQEPPGAATLGAPPQQPPSSSGAGGADELARRRKRRERRDRPPEPGTLRAQKSEEENSERKGDAVCPWDDESESAADDAPYVKTYSTLGYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VDY1</accession>
    <name>A0A6E8VDY1_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cerebellar degeneration-related protein 2-like</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CDR2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VDY1"/>
    <dbReference type="EnsemblMetazoa" id="ACON000264-RB">
        <property type="protein sequence ID" value="ACON000264-PB"/>
        <property type="gene ID" value="ACON000264"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012743"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000264"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034468"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026079">
        <property type="entry name" value="CDR2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19232">
        <property type="entry name" value="CENTROCORTIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19232:SF7">
        <property type="entry name" value="CENTROCORTIN, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="133"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="149"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="184"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="226"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000264-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="440" mass="49881" checksum="B0F6DF365650198F" modified="2020-08-12" version="1">MSGLTSDVYDEHLVDESSQYMLDDLQLAAELGKTLLERNKELEATLKHHQNIIEDHVQEIEYLTKQNAALREVNDSRMKIYEQLEVSIQDLERDKYKLALEYQAEKKHVKQLCSNIESLETKVEELTRSLEDARRQVEADRRTKKTERLLGQQQQQQQQQLPGQPAIVINSPTSHASKDAIPNVPSQEGVSTSRQTTEPCQSAKPMPAGAARQSDPAAPSAGAGEPRTEEDGTEQCERDGGPTADDHTLSEDSEEMLRIMQELERTQKCLLSEQGKVGDLEEQLAVIAQENTALQSKLLNVHSTEIRSVHDELSMLEEFRQGPVCTRCLRDLGEQQDEDSIITGTTEDDASLMELIRNTDFPQAYRSSVTIEAKTCTICAHSSKSLHELPVNVVRLLLELWLLVVRKFTILKRMLHYLYRPALLIHQLISLPTYEQLDYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VE99</accession>
    <name>A0A6E8VE99_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Protein kinase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. Lammer subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VE99"/>
    <dbReference type="EnsemblMetazoa" id="ACON000847-RA">
        <property type="protein sequence ID" value="ACON000847-PA"/>
        <property type="gene ID" value="ACON000847"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000199"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000847"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039891"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043484">
        <property type="term" value="P:regulation of RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14134">
        <property type="entry name" value="PKc_CLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000145">
        <property type="entry name" value="Dual specificity protein kinase CLK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051175">
        <property type="entry name" value="CLK_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45646">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE DOA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45646:SF11">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE DOA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="745"/>
            <end position="1059"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="77"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="110"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="168"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="360"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="454"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1071"/>
            <end position="1096"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="110"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="124"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="168"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="196"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="238"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="272"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="379"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="500"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="573"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="593"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1086"/>
            <end position="1096"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="774"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000847-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1096" mass="122143" checksum="C9A2EA4BD9CE81E0" modified="2020-08-12" version="1">MAASTTTTTTVTPSSSSTGASASSTSSRLSAERLTSLGTTSGGPSTRRRYIRSNTASVTQLLSDSCSSILQRFRRNPSEKLEQHQPQHGQTTQQKRSQFRGFNELTTSASTSSILSHGSGSATATSDYGSLGSSTDSVHRSPYLSSFNSTMSSYYKPLFRTFGKRFDSPTASSGASTASTHQLHASSNSSSSITRSRRESTTEDKDKTPLVVPGKASGAASGNSSTGGAGTGSTISRLESKYSDILDRVHRRKEQEDKEKTLEPPSGSAYHQQQQLPQTQRLLNPLMKSSTTASIVRDKSYSSAKERTPYKLQLLLGSSRSSKLSSGTSSTAMVDSLYDRNGRTYGAADRSSGMRTFGAKESSYHQHHHHHQQQQQHGEGGKENVFKSKYDPTELLSEVSGMPGSGGKSRRTIKPYKRSDTTDMGHLLHSHQAHQYGQPYGDGSLADGSAFSVSASSLTTKKERRKSTAGGGRFFDADGICTVPLSSDDDRDSSGAADATDSKDPRQRERQNRRREIESLLQKYAPLDDPGRQQLLQQQPPQQPRRERRSHATVNPSTTERSPRLEDDVASLLLQSGTAYQRQRTSSSHVRMYYQQQHQQQHQQHQQQQLQQQLYGTATTGTALGGGLQKSYTVQNVASHLLNGAGHQLHHHYGQQLPYGGGAYHHHAAPVGGSILPINTGAAHAYNSRTAAALAAGSTGLVQNQLRGPRSRIPKALSTFKQPFIRDDADGHLIYQIGDILHNRYKILATLGEGTFGRVVKVKDLERNHVMALKIIKNVEKYRDAAELEIGALEKIMQLDPNLEHLCVKMLDWFDYHGHTCIAFEMLGLSVFDFLKDNNYEPYPIEHVRHISYQLCYAVKFLHESRLTHTDLKPENILFVDSEYTTTTVPRKNREVRRVNCTDIRLIDFGSATFDDEHHSTIVSTRHYRAPEVILELGWSQPCDVWSIGCIMFELYQGVTLFPTHDNREHLAMMERILGTIPYRMARQTRSKYFRYGKLDWDEKSSTGRYVRDNCKPLHRCVITDKPDHLQLFDLIRKMLEYEPSKRITLDKALRHPFFAKLPAHQRLHEKCNDSPVGAHSSTANAERERSHSLSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VEN9</accession>
    <name>A0A6E8VEN9_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Alpha-actinin, sarcomeric</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">F-actin cross-linking protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the alpha-actinin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040224700.1">
        <property type="nucleotide sequence ID" value="XM_040368766.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040224701.1">
        <property type="nucleotide sequence ID" value="XM_040368767.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040224703.1">
        <property type="nucleotide sequence ID" value="XM_040368769.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040224704.1">
        <property type="nucleotide sequence ID" value="XM_040368770.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040224705.1">
        <property type="nucleotide sequence ID" value="XM_040368771.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040224706.1">
        <property type="nucleotide sequence ID" value="XM_040368772.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_049466880.1">
        <property type="nucleotide sequence ID" value="XM_049610923.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_049466881.1">
        <property type="nucleotide sequence ID" value="XM_049610924.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VEN9"/>
    <dbReference type="EnsemblMetazoa" id="ACON001497-RB">
        <property type="protein sequence ID" value="ACON001497-PB"/>
        <property type="gene ID" value="ACON001497"/>
    </dbReference>
    <dbReference type="GeneID" id="120950598"/>
    <dbReference type="KEGG" id="acoz:120950598"/>
    <dbReference type="CTD" id="31166"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001185"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001497"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032575"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd21214">
        <property type="entry name" value="CH_ACTN_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21216">
        <property type="entry name" value="CH_ACTN_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00176">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000004">
        <property type="entry name" value="Actinin alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000001">
        <property type="entry name" value="Actinin alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000004">
        <property type="entry name" value="Actinin alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000005">
        <property type="entry name" value="Actinin alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000005">
        <property type="entry name" value="Actinin alpha 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000002">
        <property type="entry name" value="Actinin, alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000003">
        <property type="entry name" value="Actinin, alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000148">
        <property type="entry name" value="alpha-actinin, sarcomeric isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001589">
        <property type="entry name" value="Actinin_actin-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014837">
        <property type="entry name" value="EF-hand_Ca_insen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018159">
        <property type="entry name" value="Spectrin/alpha-actinin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002017">
        <property type="entry name" value="Spectrin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11915">
        <property type="entry name" value="SPECTRIN/FILAMIN RELATED CYTOSKELETAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08726">
        <property type="entry name" value="EFhand_Ca_insen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00435">
        <property type="entry name" value="Spectrin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01184">
        <property type="entry name" value="efhand_Ca_insen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00150">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46966">
        <property type="entry name" value="Spectrin repeat"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00019">
        <property type="entry name" value="ACTININ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00020">
        <property type="entry name" value="ACTININ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="4" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="10">
        <location>
            <begin position="36"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="10">
        <location>
            <begin position="149"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="11">
        <location>
            <begin position="751"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="11">
        <location>
            <begin position="792"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="270"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00043249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00067262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001497-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="897" mass="103690" checksum="5FECF7DBFE6A2100" modified="2020-08-12" version="1">MMENGGYVGQYGGEENYMEQEEEWEREGLLDPAWEKQQKKTFTAWCNSHLRKAGTSIENIEDDFRNGLKLMLLLEVISGETLPKPDRGKMRFHKIANVNKALDFIASKGVKLVSIGAEEIVDGNLKMTLGMIWTIILRFAIQDISVEEMTAKEGLLLWCQRKTAPYKNVNVQNFHLSFKDGLAFCALIHRHRPDLIDYSKLSKDNPLENLNTAFDVAEKYLDIPRMLDPDDLINTPKPDERAIMTYVSCYYHAFQGAQQAETAANRICKVLKVNQENERLMEEYERLASDLLEWIRRTMPWLNSRQSDSTLAGVQKKLEEYRTYRRKHKPPRVEQKAKLETNFNTLQTKLRLSNRPAYMPTEGKMVSDITNSWKGLEHAEKAFEEWLLAETMRLERLEHLAQKFKHKADTHEDWTKGKEEMLQSQDFRNCKLNELKALKKKHEAFESDLAAHQDRVEQIAAIAQELNTLEYHDCASVNARCQRICDQWDRLGALTQRRRQGLDEAERILEKIDLLHLEFAKRAAPFNNWLDGAREDLVDMFIVHTMEEIQGLIQAHDQFKATLGEADKEFNVIIGLVRDAEAIVKQEQVPGGLVNPYTTLSADLISRKWSEVRALVPQRDQTLANELRKQQNNEMLRRQFAEKANAVGPWIERQMDAVTAIGMGISGSLEEQLHRLKEYEQAVYAYKPSIEELEKIHQAVQESMIFENRYTHYTMETLRVGWEQLLTSINRNINEVENQILTRDSKGITQEQLTEFRSSFNHFDKNRTGRLAPEEFKSCLVSLGYSIGKDKQGDMDFQRILAVVDPNASGYVQFDAFLDFMTRESTDTDTAEQVIDSFRILASDRPYILPDELRRELPPDQAEYCIQRMPPYKGPNAIPGALDYMSFSTALYGESDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VGA1</accession>
    <name>A0A6E8VGA1_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="5">BTB domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VGA1"/>
    <dbReference type="EnsemblMetazoa" id="ACON003439-RB">
        <property type="protein sequence ID" value="ACON003439-PB"/>
        <property type="gene ID" value="ACON003439"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005866"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003439"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034718"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18315">
        <property type="entry name" value="BTB_POZ_BAB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000036">
        <property type="entry name" value="Mod(Mdg4), isoform H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
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                <person name="Lambert C.C."/>
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                <person name="Galvin B.D."/>
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</entry>
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    <accession>A0A6E8VGA8</accession>
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                <person name="Galvin B.D."/>
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            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
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        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
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        <citation type="submission" date="2020-05" db="UniProtKB">
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                <consortium name="EnsemblMetazoa"/>
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        <scope>IDENTIFICATION</scope>
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        </source>
    </reference>
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        <subcellularLocation>
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        </subcellularLocation>
    </comment>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="222"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="294"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003439-PL"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="457" mass="50608" checksum="B0021848E0321C62" modified="2020-08-12" version="1">MADDEQFSLCWNNFNSNLSAGFHESLQRGDLVDVTLAAEGHLVKAHRLILSVCSPYFRKMFTQVPVNQHAFIFLKDVSHSALQDLIQFMYCGEVNVKQDALPAFISTAEALQIKGLTETGDSAPTHQSPAKEEPAAAAAVPVTTATISTATIPASPASQRAKVQRNRIQSYKLESEESGDDKVVHIQATTSHHVSAQSNLSSQKRTMPQRGLQSHASKRTKMSISASSDGLDTSDSTPAQVQTVQTVQIVKQIPAQVIEPEYIELPIESINPKAEPDYTDETAEIETVDAETEQEQKLSEHDQGDADDDGNYVEDDTYGDMAMGKYEESYLTEGEEGAKPGVSGFVDSYTSDGGNATEISTQVNPHIEIYTTKSFRGRPAIIVDKQKYLLMSENSKRIVWRCSSMATEKLKCPARIMQYKDTDQYTFPDKSVHQHAPLKRYKNINCQSSIDIMMHHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VGS2</accession>
    <name>A0A6E8VGS2_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Methyltransferase-like protein 9</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_049466874.1">
        <property type="nucleotide sequence ID" value="XM_049610917.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_049466875.1">
        <property type="nucleotide sequence ID" value="XM_049610918.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VGS2"/>
    <dbReference type="EnsemblMetazoa" id="ACON003602-RB">
        <property type="protein sequence ID" value="ACON003602-PB"/>
        <property type="gene ID" value="ACON003602"/>
    </dbReference>
    <dbReference type="GeneID" id="120950058"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_008649"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003602"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039912"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106370">
        <property type="term" value="F:protein-L-histidine N-pros-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007884">
        <property type="entry name" value="METL9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12890">
        <property type="entry name" value="DREV PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12890:SF0">
        <property type="entry name" value="PROTEIN-L-HISTIDINE N-PROS-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05219">
        <property type="entry name" value="DREV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003602-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="304" mass="35688" checksum="4C5567B99B73F7CB" modified="2020-08-12" version="1">MSNYRPRGAIAKVFFDRLHNDDVIQQIDMTTWYTMGKLPRKYGSLYHHLNGPDELTLEWLERSKKTSGRFWLQLCHEVAKLFLNMFMTQTDINGFLKRGSMFILSEMQFDQFLTAGGFFQHRERQSALSVCDIGAGDGEVTLRLVHTLQQKRNWQVTTYATESSWTMRNRLNEKNFIVLENLNQIHQADLIVCFNVLDRCFDPHVMLSDIYESLDQTGFALIALVLPYSHYVEKNSSHLPLRTLLEPWPPEKRLTVEEELEMFFDVLQAVGFKIRTWTKAPYLCEGDLRQSFYWLTDYVVLCSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VH42</accession>
    <name>A0A6E8VH42_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Extended synaptotagmin-like protein 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the extended synaptotagmin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VH42"/>
    <dbReference type="EnsemblMetazoa" id="ACON003725-RA">
        <property type="protein sequence ID" value="ACON003725-PA"/>
        <property type="gene ID" value="ACON003725"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012206"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003725"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032373"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031210">
        <property type="term" value="F:phosphatidylcholine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008429">
        <property type="term" value="F:phosphatidylethanolamine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061817">
        <property type="term" value="P:endoplasmic reticulum-plasma membrane tethering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08391">
        <property type="entry name" value="C2A_C2C_Synaptotagmin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04050">
        <property type="entry name" value="C2B_Synaptotagmin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04030">
        <property type="entry name" value="C2C_KIAA1228"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21670">
        <property type="entry name" value="SMP_ESyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000093">
        <property type="entry name" value="Extended synaptotagmin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000155">
        <property type="entry name" value="extended synaptotagmin-2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000158">
        <property type="entry name" value="extended synaptotagmin-2 isoform X4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037752">
        <property type="entry name" value="C2C_KIAA1228"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037733">
        <property type="entry name" value="Ext_Synaptotagmin_C2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037749">
        <property type="entry name" value="Ext_Synaptotagmin_C2B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051634">
        <property type="entry name" value="Extended_Synaptotagmin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031468">
        <property type="entry name" value="SMP_LBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039010">
        <property type="entry name" value="Synaptotagmin_SMP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45761">
        <property type="entry name" value="EXTENDED SYNAPTOTAGMIN-LIKE PROTEIN 2, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45761:SF1">
        <property type="entry name" value="EXTENDED SYNAPTOTAGMIN-LIKE PROTEIN 2, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17047">
        <property type="entry name" value="SMP_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51847">
        <property type="entry name" value="SMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="13" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SMP-LTD" evidence="17">
        <location>
            <begin position="5"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="16">
        <location>
            <begin position="184"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="16">
        <location>
            <begin position="323"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="16">
        <location>
            <begin position="596"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="468"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="567"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="489"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="584"/>
            <end position="595"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003725-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="20">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="728" mass="80569" checksum="406B4CD960A14AFD" modified="2020-08-12" version="1">VFFPDVERCEWLNRILKQVWPNANFYAKNLIKESIEPNIQQAMAGYKLNGFKFDRMILGTIPPRIGGVKVYDKNVSRNEIIMDLDLFYAGDCDISFALSGLRGGIKDFQIHGTVRVIMKPLISQMPLIGGLQIFFLNNPNIDFNLVGVVDLLDMPGLSDILRKIIVEQVAAIMVLPNKLPIVLSDGVPALSLKMPEPEGVLRIHVVEAKDLMKKDISVLGKGKSDPYAIISVGAQQFRTQTIDNTVNPKWDYWCEAFIHAESGQTLQVVINDEDAGEDELLGRATVEISSVTKNGEIDTWLTLEQAKHGLVHLRMTWFKLSSEKSDLKQALEETQHLRVTSMSTALLTVFIDSAKNLPQARQQSQPDPYLVLSVGKKNEQTSVQMRTDAPVWEQGFTFLVGNPDNDTLQLKVIDQKTGNTIGTLTYILSALMEKKNLEIMSQPFQLQKSGPETKIIMSLSLRILKRHREQEPAVTTPDKGPASEADSVLSRTSSIRTSASHGSQSGTLQQQPSTGDSNAAEAAALSHQGSVRKQDSRKSTTSAIMEQMSIQEEPFVVSTLNTVMMATPPRSPNLSDGGTELLRRSPSTTSSSGSAGLGRIQLTVAYSVQRQRLLVIVHKINNIPLKDPNNIPDPYVKLYLLPGRSKESKRKTNVVKDNCDPVFDTTFEYIISNAELVNSELEVTVCTQKGFFGSPVIGMQKLSLSDPDISSGQGIKAWYDLLPESKFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VIB1</accession>
    <name>A0A6E8VIB1_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Fibroblast growth factor</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the heparin-binding growth factors family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VIB1"/>
    <dbReference type="EnsemblMetazoa" id="ACON004181-RB">
        <property type="protein sequence ID" value="ACON004181-PB"/>
        <property type="gene ID" value="ACON004181"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000757"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004181"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_029746"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23311">
        <property type="entry name" value="beta-trefoil_FGF_Bnl-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002209">
        <property type="entry name" value="Fibroblast_GF_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008996">
        <property type="entry name" value="IL1/FGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11486">
        <property type="entry name" value="FIBROBLAST GROWTH FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00167">
        <property type="entry name" value="FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00442">
        <property type="entry name" value="FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50353">
        <property type="entry name" value="Cytokine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026146085" evidence="3">
        <location>
            <begin position="30"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="68"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="124"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="442"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="712"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="155"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="242"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="447"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="483"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="516"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="561"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="619"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="634"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="650"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="719"/>
            <end position="729"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004181-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="787" mass="84277" checksum="4BDE2362EA68EBAC" modified="2020-08-12" version="1">MLSGRIRRRALALLGALLLVILVWPPCCATMPKPLSVTSPQAPWDVVPPARPHPAAAVMPVPVHPSWTSGEGTFAPKRHHVPIGPGSERHQNEQHQQQQQEPAESESRKSRRVSEFYRLLRALSPATQPRPTFEPNERTQSEVSAAVQLNLPPRENSRAGRVARDYLIEPIDKNKSQSARPASALDLPQDNAPVRVHARFDDTNRSPALSRSRREALPANPHRHQHQLAPAGAPMNGTGVWHGRRGGGGHRHHHQHHQQQQQQNRNIESNLERNERSANLSHITGATRKIQLFIKNRYIQLLPDGTVNGTSDDLSDYKEFTDDCIFNEQMEQHHYNTYSSTQHSNARRTLYLALNKTGQPRKIQIPVNRTLGKLATYTKALTLTVAHDRVEQLISRLFGADHVRHGLNHLCETGQPLVELTAKEMRPRPVCGAQAGPMKGIAAATSTGGGGGGGGKKNGQQNTAGKDEKEGLRQKKKKKRRKCREDEVPGEHCFRPGASGAGVGGTNSGKKKAGPNQGQSRGNSSISSDSGSISPSGSNSSVGSSSAGTNQSASLNNVTGSSSSSSSSSSSSSSSSISNSSSANSLIASISNGGPVRPGQPSAQLTATPNARKPKPTKKLPGGPRNGTQPPSGGNTGPTGGGSSRKPKKPNQQSQNQQQQMANGKRNGTGGNSAKPSPAKIKLVGASIVTSKLSHPKPASLMIARTVRPTTPFPLVTARPTSGPTTVSNDLLDTSQQSDDSELDMDDALQASAELVDELDDLDEGMLTVPGHAINFVDEEDLDDMEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VID5</accession>
    <name>A0A6E8VID5_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VID5"/>
    <dbReference type="EnsemblMetazoa" id="ACON004179-RC">
        <property type="protein sequence ID" value="ACON004179-PC"/>
        <property type="gene ID" value="ACON004179"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_003960"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004179"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042095"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990246">
        <property type="term" value="C:uniplex complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036444">
        <property type="term" value="P:calcium import into the mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051560">
        <property type="term" value="P:mitochondrial calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15900">
        <property type="entry name" value="EFh_MICU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039800">
        <property type="entry name" value="MICU1/2/3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12294">
        <property type="entry name" value="EF HAND DOMAIN FAMILY A1,A2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12294:SF13">
        <property type="entry name" value="MITOCHONDRIAL CALCIUM UPTAKE 3, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="176"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004179-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="276" mass="32960" checksum="EAFDEB59D8902503" modified="2020-08-12" version="1">MEKIFSFAWKGKRGIESKEGEEPGEHDDYVDDEQGLQRKHKVDTTLQIHFFGKKGDKDLQYDGFYNFMKNLQTEVLELEFCEFSKGHERISEVDFAKILLRYTYLDTDEYDNYLDRLLDREAKEKGVSFDEFRQFCQFLNNLDDFSIAMRMYTLADQPISKDEFSRAVKICTGSELNSHLIDTVFAIFDEDGDGLLSYKEFIAIMKDRVHRGFKVSHYEHIVEDYLDDIEKGTVYADNFRQLLKMRSRACRRLRRYLLYFYYHPDHGQSDSTRHYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VIE3</accession>
    <name>A0A6E8VIE3_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Diacylglycerol kinase</fullName>
            <shortName evidence="13">DAG kinase</shortName>
            <ecNumber evidence="13">2.7.1.107</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a 1,2-diacyl-sn-glycerol + ATP = a 1,2-diacyl-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10272"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.107"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the eukaryotic diacylglycerol kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.107" evidence="13"/>
    <dbReference type="RefSeq" id="XP_040241439.1">
        <property type="nucleotide sequence ID" value="XM_040385505.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VIE3"/>
    <dbReference type="EnsemblMetazoa" id="ACON004209-RB">
        <property type="protein sequence ID" value="ACON004209-PB"/>
        <property type="gene ID" value="ACON004209"/>
    </dbReference>
    <dbReference type="GeneID" id="120961628"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_003893"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004209"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032567"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004143">
        <property type="term" value="F:ATP-dependent diacylglycerol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20803">
        <property type="entry name" value="C1_DGKtheta_typeV_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20804">
        <property type="entry name" value="C1_DGKtheta_typeV_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20854">
        <property type="entry name" value="C1_DGKtheta_typeV_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17111">
        <property type="entry name" value="RA1_DAGK-theta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01783">
        <property type="entry name" value="RA2_DAGK-theta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.40:FF:000004">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000200">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000002">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000058">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10330:FF:000011">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020454">
        <property type="entry name" value="DAG/PE-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037607">
        <property type="entry name" value="DGK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056392">
        <property type="entry name" value="DGKtheta_RBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000756">
        <property type="entry name" value="Diacylglycerol_kin_accessory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001206">
        <property type="entry name" value="Diacylglycerol_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000159">
        <property type="entry name" value="RA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11255">
        <property type="entry name" value="DIACYLGLYCEROL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11255:SF54">
        <property type="entry name" value="DIACYLGLYCEROL KINASE THETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00609">
        <property type="entry name" value="DAGK_acc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00781">
        <property type="entry name" value="DAGK_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00788">
        <property type="entry name" value="RA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24099">
        <property type="entry name" value="RBD_DGKtheta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00008">
        <property type="entry name" value="DAGPEDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00045">
        <property type="entry name" value="DAGKa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00046">
        <property type="entry name" value="DAGKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00314">
        <property type="entry name" value="RA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50146">
        <property type="entry name" value="DAGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50200">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="15">
        <location>
            <begin position="5"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="15">
        <location>
            <begin position="68"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="15">
        <location>
            <begin position="134"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-associating" evidence="17">
        <location>
            <begin position="376"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="domain" description="DAGKc" evidence="16">
        <location>
            <begin position="565"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="242"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="704"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="921"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="242"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="263"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="714"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="938"/>
            <end position="949"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004209-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="949" mass="106672" checksum="A994F30B3C1EEDD4" modified="2020-08-12" version="1">MAGGEHTFGRKTFHKPTYCHHCSDLLWGLIGQGYICEVCNFIVHEKCVTNIVTPCTGVAPYLIRNPVAHCWSEPSHHKRKFCSVCRKRLDETPAVHCLICEHFAHVECQDFAIPDCKENATYVPGKELGHVKHQHHWREGNLPQSSKCAYCKKTCWSYECLTGYRCEWCGTTTHGGCRNNISAECTFGTLQPIYLPPHAVSIPRTEVPMEAIIGVQNRNKGTPGLQRDYSCPRSISEEITTENRYRDDEYVPRGESSGSGTHSKPDSAHKPDKDKDKKDKDKEERDEETIKVFDGNCSYRRRIFRAINVPRTCSLEQLLTTALRAFHIARDPNLFFLTDVYGDEVRLQDPTPVPGLHRVEGKRPAIYLRFRDKENDHGFVRVYPGKLQVENAYVIIPVDNDTTVKDLICESLKKFGLQSHQIEDYRCSEILLDRGVTERVLSWNERPWEIMKQLGKDSIRQMELMRFYLQLKQDPHGPNLALFVGNLPPGLSQRNYEHILTEFLGFENKFSSIGPIYYEYGSLVITYENASKAVRAFQALRESKYEEKHLLVLLLPNIEPSMVPAGVQPLLVFVNVKSGGCQGLELISSFRKLLNPYQVFDLDNGGPLPGLYVFRHIQDYKILVCGGDGTIGWVLQCLDNVGQDSECSSPPCAIVPLGTGNDLARVLRWGAGYTGGEDPLNLLRDVIDAEEIRLDRWTVVFHPEDKPEDATPKAQPNSTVVGGAQSEDNSQIFVMNNYFGIGIDADLCLDFHNAREENPNKFNSRLHNKGVYVKMGLRKMVGRKMVKELHKELRLEVDGKVVELPPVEGIIILNILSWGSGANPWGPEKEDQFSKPNHWDGMLEVVGVTGVVHLGQIQSGLRSAMRIAQGGHIKIHLHSDIPVQVDGEPWVQSPGDVVVLKSALKATMLKKIKSKRRLTEPHISPAVAGFSQGGAPENGDRDKDALSGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VKF8</accession>
    <name>A0A6E8VKF8_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">CARMIL C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CARMIL family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040229901.1">
        <property type="nucleotide sequence ID" value="XM_040373967.2"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON004841-RC">
        <property type="protein sequence ID" value="ACON004841-PC"/>
        <property type="gene ID" value="ACON004841"/>
    </dbReference>
    <dbReference type="GeneID" id="120953745"/>
    <dbReference type="CTD" id="35715"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010900"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004841"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042041"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034315">
        <property type="term" value="P:regulation of Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000009">
        <property type="entry name" value="F-actin-uncapping protein LRRC16A isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031943">
        <property type="entry name" value="CARMIL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041245">
        <property type="entry name" value="CARMIL_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051279">
        <property type="entry name" value="PP1-Reg/Actin-Interact_Protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24112:SF66">
        <property type="entry name" value="LEUCINE-RICH REPEAT, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24112">
        <property type="entry name" value="LEUCINE-RICH REPEAT, ISOFORM F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Carm_PH"/>
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        <property type="entry name" value="CARMIL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CARMIL pleckstrin homology" evidence="11">
        <location>
            <begin position="25"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="CARMIL C-terminal" evidence="10">
        <location>
            <begin position="775"/>
            <end position="1073"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="944"/>
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        </location>
    </feature>
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            <begin position="1047"/>
            <end position="1068"/>
        </location>
    </feature>
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        <location>
            <begin position="1093"/>
            <end position="1112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1118"/>
            <end position="1204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
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            <begin position="1279"/>
            <end position="1355"/>
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        <location>
            <begin position="944"/>
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            <begin position="1144"/>
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            <begin position="1289"/>
            <end position="1299"/>
        </location>
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            <begin position="1300"/>
            <end position="1321"/>
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            <begin position="1322"/>
            <end position="1340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00007298"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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            <dbReference type="ARBA" id="ARBA00022490"/>
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            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="Pfam" id="PF16000"/>
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            <dbReference type="Pfam" id="PF17888"/>
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            <dbReference type="EnsemblMetazoa" id="ACON004841-PC"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1402" mass="153215" checksum="2FFF14A5D063943B" modified="2020-08-12" version="1">MSTRSQLTKDLNESVKTLLGRHTKILVKYMVKLEVKSDKTENRVLVFTPVRVYLLTAKVPTRIDCHFHYLDIQSIESRKPTHFTITTNDKSYSFSTIGDAGSFTSNADVILTDLSSAIKQIFPTVPLRYIIRKIDVNPIERTSIFSDELRPSDPRNVGPCGGFSMQYACMCDLHAVQYREEVAWDIDTIYLSHDARVLNLRDFDHLEQKDLMAIVAALEYNTFFRGLKASHTRLSTETLERVLQVLRRSLWLEELHLESLGLKSDFIHKLAVAVISNSAPALRSIDLSHNVIEDKGATHLAGPIAKMSKGFSKLALAHCGLTAKGVNQLAHSLSLNQNISNSLTYLDLSGNILKDDGNNLYNFLAQPNVIEHLDISRTDTTLESVFGALLRGCATHLLHLNVSHNNFGTKKGKEIPPSFKQFFTSSLSLKHLNIASCKLPLEALKHLLLGLACNESTAGLYLDLSGNSLGSQGAHVLESCIHGVRVLACLDISDNNMDYELANVLTAIGKNPSIRSLYMSKCFTGMKLKHIGTVMDSLVNLIQKEDFQLQELVLAENRLKTDLHNFINALGSNQHLQMLDITGNLMGDIGARLLAKALQINNKLKTIALDRNNFTLQGYADIVYALENNFSVRNMPFPLHDIAPCLKQHPERTDVLMKKMQELLFRNGNGFKRTHGQGFRLQHGFLLSSTHQVLDKLVAETQETMSSRQSSLDGSLSRLLEDAENCKQLLPKLQESVRCDPHPIEVRLGKMKTELHHSIKSYLEETMETMLRTGIEQCPKTLGSQVVLTELRKGCEERLTISEEFLQSCLVNSAGGEVMNRISEVEQSLASLISDRATDEVLEALTRYRKGLGIAEGPLSYTDDLHSLDGSKHNSSQSTEPIANFELRGLDLPKLGLDSPTATPQLQHKRRSIARKVRPQSVVGLGIENLSLANIPDVTETSMASVSRSSSNNSGSKKYESSNAKEDECCDSITELPSQSLTLQHLVKGRPKRAKTRAPKKPVITDTVTAGGTVVNEGIESFFRPGSATPSTLTPLVSPTSEECSSLSFVDSPTLSRDDGGRFSNVTSGETTPILEERKPLKLDRSSPLLKGVNWTSRSRSSDNLEKVSPSVNRKSPLVKAATEVVETPVRESRPVDNGAATPITGSKSPSNESVRNHHVESAHGASRLGNGTGQKTPIITPKPRPWSVLGHESKNEFNEPSVDSIDTDGDLVLISGQTPGGSIVGITPGAISSAGVTGGSILGITPGAVIEKKSVRDMAAGLNKLGVELERNSLMRSSISSHSASAVMRTSPSSILGRSSQKNNSMTSASSSSSSSPTSSVKNGDSTTSDGAPTISSGANIEETGAISVPQKDEKHVKRIVAGKKITALFEETLVEGLQKSSRKTMYRESTVYKDEDTVDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VKH0</accession>
    <name>A0A6E8VKH0_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Protein furry</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="EnsemblMetazoa" id="ACON004892-RA">
        <property type="protein sequence ID" value="ACON004892-PA"/>
        <property type="gene ID" value="ACON004892"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005549"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004892"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_036227"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030427">
        <property type="term" value="C:site of polarized growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031175">
        <property type="term" value="P:neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025614">
        <property type="entry name" value="Cell_morpho_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025481">
        <property type="entry name" value="Cell_Morphogen_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045842">
        <property type="entry name" value="Fry_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039867">
        <property type="entry name" value="Furry/Tao3/Mor2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029473">
        <property type="entry name" value="MOR2-PAG1_mid"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12295">
        <property type="entry name" value="FURRY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12295:SF30">
        <property type="entry name" value="PROTEIN FURRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19421">
        <property type="entry name" value="Fry_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14225">
        <property type="entry name" value="MOR2-PAG1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MOR2-PAG1_mid"/>
        <property type="match status" value="4"/>
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    <dbReference type="Pfam" id="PF14222">
        <property type="entry name" value="MOR2-PAG1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cell morphogenesis protein N-terminal" evidence="2">
        <location>
            <begin position="209"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="domain" description="Cell morphogenesis central region" evidence="4">
        <location>
            <begin position="1465"/>
            <end position="1638"/>
        </location>
    </feature>
    <feature type="domain" description="Cell morphogenesis central region" evidence="4">
        <location>
            <begin position="1672"/>
            <end position="1754"/>
        </location>
    </feature>
    <feature type="domain" description="Cell morphogenesis central region" evidence="4">
        <location>
            <begin position="1858"/>
            <end position="1920"/>
        </location>
    </feature>
    <feature type="domain" description="Cell morphogenesis central region" evidence="4">
        <location>
            <begin position="2015"/>
            <end position="2115"/>
        </location>
    </feature>
    <feature type="domain" description="Cell morphogenesis protein C-terminal" evidence="3">
        <location>
            <begin position="2281"/>
            <end position="2533"/>
        </location>
    </feature>
    <feature type="domain" description="Protein furry C-terminal" evidence="5">
        <location>
            <begin position="2569"/>
            <end position="2829"/>
        </location>
    </feature>
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        <location>
            <begin position="2901"/>
            <end position="3180"/>
        </location>
    </feature>
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        <location>
            <begin position="749"/>
            <end position="775"/>
        </location>
    </feature>
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            <begin position="843"/>
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        <location>
            <begin position="1142"/>
            <end position="1169"/>
        </location>
    </feature>
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        <location>
            <begin position="2172"/>
            <end position="2207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2600"/>
            <end position="2659"/>
        </location>
    </feature>
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        <location>
            <begin position="2713"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2763"/>
            <end position="2879"/>
        </location>
    </feature>
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        <location>
            <begin position="3128"/>
            <end position="3150"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="3315"/>
            <end position="3371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="749"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="759"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="843"/>
            <end position="852"/>
        </location>
    </feature>
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        <location>
            <begin position="853"/>
            <end position="866"/>
        </location>
    </feature>
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        <location>
            <begin position="2198"/>
            <end position="2207"/>
        </location>
    </feature>
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        <location>
            <begin position="2606"/>
            <end position="2618"/>
        </location>
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        <location>
            <begin position="2637"/>
            <end position="2649"/>
        </location>
    </feature>
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        <location>
            <begin position="2713"/>
            <end position="2731"/>
        </location>
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        <location>
            <begin position="2819"/>
            <end position="2832"/>
        </location>
    </feature>
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        <location>
            <begin position="3129"/>
            <end position="3141"/>
        </location>
    </feature>
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        <location>
            <begin position="3320"/>
            <end position="3335"/>
        </location>
    </feature>
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        <location>
            <begin position="3345"/>
            <end position="3354"/>
        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14225"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF14228"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF19421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004892-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="3379" mass="369895" checksum="5EE9DC93F5E37787" modified="2020-08-12" version="1">MQLSQLAPGSGSGGLSACGLSGANSTVVDEGDTITVIERPVHIETSEPSKSPLGVVNTTSVRLSLLPWNSHNRSAILSGNSGSGMVTTIDMMAASDGTLRPGEIVMRTLFTDFTVQAEKKIECVMLDGSDRNLPKLLQRGEDYQFDQLLQALGSVAEHCLPSLLKALLAWHKHQICDKNIKRDLQTGASSTAHHPANKSGLDLDYQLLRREAAVEFIFCLALIEILRQFPFHPGHDDITKQIENLAFRQFKYKEGAQTAPNANDNYRIADLYAEVVGVLAQSRFSSVRKRFMTELKELRSKEPSPFTTHSIISLLMGMKFFRVKMAPIEEFEASFQFMHECAQYFLEVKDKDIKHAMAGLFVEILVPVAATVKNEVNVPCVKNFIDLLYSQTLDACTKSKHKLALFPLVTCLLCVSQKSFFLSNWHCFLAMCLSNLKNKDPKMSRVALESLYRLLWVYIIRIKCESNSATHSRLQSIVNSLFPRGSKAVVPRDTPLNIFVKIIQFIAQERLDFAMREIVFELLCVGRPIKIIMTPERMSIGLRAFMVVADSLQQKDGEPPMPRTVGVLPSGNTLRVKKTYLNKMLTEDTARSIGMSNYFPHVRRVFVDILRALDIHCGRPLMMTVIQNQNKELDEMLTGERKPRIDLFRTCIAAVPRLTPDNMTGHELVDMLSRLTIHMDEELRGLAHQSLQTLVCDFPEWRQDVIQGFSQFLARDVVDTFPQLLDNGLRMLYAFLTVWRNLLGAGSNNNSNSNTLNTKGGGPAGTGGGGVPLASAGQPVIGRAANQQDLGMLKPNLTQSNVNASLNAATINSNSSGSSGISSITQTTTITAGAIGGGAGTTSSSGIGGTGDGTTNTTAAGSTTAGGKKGHELPLATTMHMVEGLALVMLCNCRSPPRKFAVSILKEVKILKKLLGIPETEPALIDVIDKCCPQVLEKCLHMLPQTDRTAMLNANIIDLQWIVDRNSGIWTTGHVEESTKASTLTLCLSQSALGQAYDPWSVCLFGFMERTRVLQHCPSVVAQAWPICYQRVTSLFNVIDPTPVSDNRASLLRSSAPTKKPPSEAQRDSLAHLWKNQVALAMRLIPQMPSVAVRCASPDLSLSLHDQSDSRSLSDSLDNIPSSVFGPEGIVTRFTLPLSYGRKDARHKRQGSSPDSLSADRSDKSSGTSASPVALYKLVIPLLRCEVVDIRDAAVNALGMINHDALKDLMEELVVYIREAIDRKQENMRRRRRRDALRLQLVRVLEKIAENGTFGISQFVLERETMSLHPTFVEYIDGARLYLETETDGKDSSSMREVKTHFCDFIRKMIKNFSLESCGTLLSRELKRNLVNLFVGWSGTAYALPFAHGHHGSSHHGSHHGVGAGGSGGGSISGIGSGVTGIGGGTIGATVWGSSSTTAGISSAANTSVGTIAAEEEKLQFSALQAMSAVLCCGPCFDPGHLAEDGAIYSWLDMLMTSKDEKIYELAKETVVLLLESNPDIGQLLEWVIDRCYTASPREADACFLALAVIFSAREYPCDHYTSVINVTLLMTGCPRVEVHSTALQLLQILDKRFFGNVGPLQTENDREPDKIGTLDAVLSGAYCRSQMYLSRQIARLRPELTMSMFSEITYRFQTARAEARALLLQCLLPWLENVELVASSVPPATPLSYIMYYPDSGTRGRREGSGSTEATEMILNNLFYITAKFADSHPYIEELWGTLCQFWPNNLKVILRYLLIISGMAPNELLANAKRVALYLARSCTNRLLDELMSELQTVETLNCLITCKSDSALRTFANAYRPPRGSDKIRTASGPCVLPRHEDVMMMMNEPEMNQEDNFVQIRTEQPTAPHPLPMPEYGGYFAPLTEFLPDTNIPISGFHRCNVAVMLLTDIVVDGIPGLDWTLHVPLMLHILFLGLDHTRRIVRDHCKKLLLNLLIVLAEHNDHLTVAKILLNSDTSKLRLGIVVPSLPVIDHNFTEPDQEFDSYLFGLNSTSTQQSVLSPASNQPNEETPAVPMIVTEENAPPQPGPTMPTAHVIKSLIKFLSNESSQQHPLWNYEDITAKVWSIKSADQLQCFLRHIVKAFTDSYPHARIPERWAQTALQLGLSCSSRHYAGRSLQVFRSLNVPINSRMLSDILSRLIETVAEQGEDMQGYVTELLLTLEAAVDSLDSDFRPLDVMKDIFKSTPNLNNKEGVVSSMYSGSKRSGDGGITGGSPGQSLHNATSGGHTRSTSYSISYCSRKAANSPIDKQIELRNRNAALEIERNAAKFGAGQTSLSRSRSAQSLKLLGDTATQDDKMTILAQLFWLAVSLLESDYEHEFLLALRLLTRVLHRLPLDRPDARDKVEKLQQQLKWSTYPGVHALLLKGCTHSGTYEASIALLSQLTPLLNLPVCDPTQSCAFPMNVIALLPYMLLHYEDANEICIRSAENIAQVASEMGLKLENLGTVMTLYSRKTFSKESFQWTKCVVKYLYDTYAHMGLHMLAFLTEVLEKGWVQVQLQVLSVIHCMLHYVDLSAVAIQPIAGDMLRIISKYLDSPNWKEALKILKFVVTRSSSLQVVPQSSEGGGGGGGGSSFANLHGSFSDSEVFCKKELAGRTMEFSFDVSQTPLIGRKLLLKSDHDTSAPSGVGGGTQMGGKGFNGSSNVTGSGAHYGGHAGTPNSPRRSASLSPADTAPLSGWKRPWMSQGRVRECLVNLLTTCGQRVGLPKSPSDDIISSLYSKVIFSQSSDLLERQSSAASSTDETSGAQQDLSGGSKRDDGAPDFGVFKDFDFLEYESESIEGESTDNFNWGVRRRPLSEGENEPHSLSAAGVRASHTNIDESLSEKTPVFNRRKRPNADDSSDEEVESESPLDEEPRPIFTKAAYLSGPPTSLSLRERRHRRDSVSRSDTSGSSAGDLGDITPCNASPHLPGMHPFRTGPIRDESVDVWRRNLVGLLVNQPSSHAPDLLNQMFRLIKELTVRSIAISKECMRYFTGCGVQLGHRISVLSDLLATRGDPPKIWYHQSLATTPRLFENLRYGVLEVQEHLETFFDRKETVLDSLDEVKTSCKLALFTSDIDSANEGTGGSSAVVSLSTSTDPATAELLLDLGRGLYKLMFQLLLLIESDHKIAISVVQNLRQNEQMQDLSNLYLSVRGALLRCIDDAEMESLDTSTSTEGENTPTPSPGLPMNNSEFENILVELIDNQRWSAAINHVKQHKQMSNHSASNYNLSGGASGSSGGGSGVGGMMFASGGGNCSITGGGGVGDGLFANLGTEFKPVDDLSIILNVYAHRLTKDRNDVFIVSRSELELSEIYQILMENLLHVSSALSNMEISMKANTSSSSSTVTTMTTIPAVIGTGPSTGPGNGGTGNGHSGAPGNAAGSLAGSTAQGSSNLPPPPPPVLAPTNPFKDTVDIITTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VKH1</accession>
    <name>A0A6E8VKH1_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Homeobox protein abdominal-A homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Antp homeobox family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VKH1"/>
    <dbReference type="SMR" id="A0A6E8VKH1"/>
    <dbReference type="EnsemblMetazoa" id="ACON004662-RB">
        <property type="protein sequence ID" value="ACON004662-PB"/>
        <property type="gene ID" value="ACON004662"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_013719"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004662"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031157"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009952">
        <property type="term" value="P:anterior/posterior pattern specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000317">
        <property type="entry name" value="homeobox protein abdominal-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022132">
        <property type="entry name" value="Abdominal-A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050296">
        <property type="entry name" value="Antp_homeobox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45659:SF4">
        <property type="entry name" value="HOMEOBOX PROTEIN ABDOMINAL-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45659">
        <property type="entry name" value="HOMEOBOX PROTEIN HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12407">
        <property type="entry name" value="Abdominal-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5 9 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 9 10" id="KW-0371">Homeobox</keyword>
    <keyword evidence="7 9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="12">
        <location>
            <begin position="136"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="9">
        <location>
            <begin position="138"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="207"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="207"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="223"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="248"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00072555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004662-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="308" mass="33137" checksum="5B25ABC97769C6F7" modified="2020-08-12" version="1">MYPFVSNHPTTHTSYSTMPGFSGLDDKSCSSRYTDSVMNSYPPMGVPGSASIAQFYQQAAAVSAASAGVGVDSLGSACSQLSSSVGGAQSGLPDITRHPWLVTASQSALQKFASTDWMSNPFDRVVCGDFAGPNGCPRRRGRQTYTRFQTLELEKEFHFNHYLTRRRRIEIAHALCLTERQIKIWFQNRRMKLKKELRAVKEINEQARREREEQDKMKNESLKSAQQHHSQKQAQQEHTVVGSQQTSNGGGTGGGTGGSGGAGSGGSSGNLGSHLHHPSIVSQNDLKLGLGGMGVGVGGNLSMMGAQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VKH3</accession>
    <name>A0A6E8VKH3_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Class II aldolase/adducin N-terminal domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldolase class II family. Adducin subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VKH3"/>
    <dbReference type="EnsemblMetazoa" id="ACON004852-RA">
        <property type="protein sequence ID" value="ACON004852-PA"/>
        <property type="gene ID" value="ACON004852"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011370"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004852"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034524"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.225.10:FF:000011">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.225.10">
        <property type="entry name" value="Class II aldolase/adducin N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051017">
        <property type="entry name" value="Aldolase-II_Adducin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001303">
        <property type="entry name" value="Aldolase_II/adducin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036409">
        <property type="entry name" value="Aldolase_II/adducin_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10672">
        <property type="entry name" value="ADDUCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10672:SF3">
        <property type="entry name" value="PROTEIN HU-LI TAI SHAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00596">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01007">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53639">
        <property type="entry name" value="AraD/HMP-PK domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Class II aldolase/adducin N-terminal" evidence="3">
        <location>
            <begin position="121"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="329"/>
            <end position="355"/>
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        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006274"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="SMART" id="SM01007"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004852-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="472" mass="52419" checksum="A2588B8E95AEB850" modified="2020-08-12" version="1">MATETETATIHTNGGLETVSEEEGMKPRPADIEADMREMERRKRVESIMNSRLFREELERIVDGQMREGSSTILQQLSDIMGMPAARIGSVFKSSNCVVPINDIRGVESMGYEKGEKILRCKLAATFRLMDLFGWTQGIACQITARLNADQELFLVNPYGMLFHEVTASSLNKVNMQGQIVEHGTTNFGINNNQFTLHSVIHAARPDIRCAIYVAYSAVTAISALKQGLQPLTKHTALLGEIAFHTYAGALNEPEEKDKLLRSLGPVSKVLLLSNHGALCCGETIEEAFYHVTHMVHACEAQLKLLPVGLENLILIPEETRKAIYDAARKPSGEGQEAASAQTADNKEGQLTKTPKWRVGGSEFEALMRMLDNAGFRTGYIFRNPLIKGDVPKPRNDIEVPPAVSSLGYLIEEEELFKQGIWRKGDPRKVGDRTRWLNSPNVYQKVEVLETGTPDPKKITKVEIVTIEGNLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VKH8</accession>
    <name>A0A6E8VKH8_ANOCL</name>
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        <submittedName>
            <fullName evidence="2">Ribosomal silencing factor RsfS</fullName>
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        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
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    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Iojap/RsfS family.</text>
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        <property type="protein sequence ID" value="ACON004955-PA"/>
        <property type="gene ID" value="ACON004955"/>
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    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035189"/>
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        <property type="component" value="Unplaced"/>
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        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="IOJAP SUPERFAMILY ORTHOLOG"/>
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        <property type="entry name" value="MITOCHONDRIAL ASSEMBLY OF RIBOSOMAL LARGE SUBUNIT PROTEIN 1"/>
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    <proteinExistence type="inferred from homology"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="212" mass="24270" checksum="52B687D86AB1E2E0" modified="2020-08-12" version="1">MANAIRRSIPKFNRALVLPAFRSFHDEFSSKIPLEDIRKQTKGKEQHVPSARSIAAKYQVFRDTDAPVILDVDEERQIHEAGLQQPEKIHDLPDMYSGINLSRGKTGVYEIEDLVTVLRLNSAINIFVCTVPKDIKYVDYMCIVTGRSVRHMRGMAEFVRKVFKMKRHPSDIIPKIEGESSSDWMALDLGNVEYAVKTLYSYTHWSSIVPCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VKI1</accession>
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        <recommendedName>
            <fullName evidence="16">Down syndrome cell adhesion molecule-like protein Dscam2</fullName>
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        <name type="scientific">Anopheles coluzzii</name>
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            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
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                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
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            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">Ngousso</strain>
        </source>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
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    <dbReference type="EnsemblMetazoa" id="ACON004902-RB">
        <property type="protein sequence ID" value="ACON004902-PB"/>
        <property type="gene ID" value="ACON004902"/>
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    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002416"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004902"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_036439"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="P:cell-cell adhesion"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:neuron projection morphogenesis"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="match status" value="6"/>
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        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20956">
        <property type="entry name" value="IgI_4_Dscam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20958">
        <property type="entry name" value="IgI_5_Dscam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20954">
        <property type="entry name" value="IgI_7_Dscam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002485">
        <property type="entry name" value="AGAP004902-PA-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000333">
        <property type="entry name" value="Down syndrome cell adhesion molecule"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Down syndrome cell adhesion molecule 2, isoform Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000017">
        <property type="entry name" value="Down syndrome cell adhesion molecule b"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000104">
        <property type="entry name" value="Down syndrome cell adhesion molecule b"/>
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    </dbReference>
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        <property type="entry name" value="Down syndrome cell adhesion molecule, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000678">
        <property type="entry name" value="Down syndrome cell adhesion molecule-like protein Dscam2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001035">
        <property type="entry name" value="Down syndrome cell adhesion molecule-like protein Dscam2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001049">
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    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001118">
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    </dbReference>
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        <property type="entry name" value="Neuronal cell adhesion molecule"/>
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    </dbReference>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056754">
        <property type="entry name" value="DSCAM/DSCAML_C"/>
    </dbReference>
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        <property type="entry name" value="FN3_sf"/>
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        <property type="entry name" value="Ig-like_dom_sf"/>
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        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
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        <property type="entry name" value="FIBRONECTIN TYPE-III DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN SIDEKICK"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25059">
        <property type="entry name" value="FN3_DSCAM-DSCAML_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="I-set"/>
        <property type="match status" value="5"/>
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        <property type="match status" value="3"/>
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        <property type="match status" value="6"/>
    </dbReference>
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        <property type="match status" value="10"/>
    </dbReference>
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        <property type="match status" value="9"/>
    </dbReference>
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        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1580"/>
            <end position="1602"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="4"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="204"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
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            <end position="383"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="389"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="487"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="578"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="674"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="772"/>
            <end position="869"/>
        </location>
    </feature>
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        <location>
            <begin position="874"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="13">
        <location>
            <begin position="971"/>
            <end position="1071"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="13">
        <location>
            <begin position="1076"/>
            <end position="1173"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="13">
        <location>
            <begin position="1177"/>
            <end position="1272"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="1274"/>
            <end position="1362"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="13">
        <location>
            <begin position="1364"/>
            <end position="1457"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="13">
        <location>
            <begin position="1458"/>
            <end position="1561"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1689"/>
            <end position="1866"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1724"/>
            <end position="1736"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1738"/>
            <end position="1747"/>
        </location>
    </feature>
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        <location>
            <begin position="1774"/>
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        </location>
    </feature>
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        <location>
            <begin position="1792"/>
            <end position="1805"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1818"/>
            <end position="1835"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1851"/>
            <end position="1866"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004902-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1866" mass="205456" checksum="D83CA56F91C017E7" modified="2020-08-12" version="1">MEPPSRLEFSNSSGGWLDCSASGSPQPSIDWLSVDGTSVGDVGGVRRVLRNGTLVLLPFPATAYRQDIHSTIYRCVASNTVGRVISRDVQVRAVVAQAYKVDVEVLAASRGCTAILRCVVPNFVKELVRVVSWVQEPAFYIYPSLQGDGKYHLLPTGELLIHNLEYNDRYPSYRCRTMHKLTRQVVTSNSAKIRMNEQRGIVSPSVVEHTSHVAVSQDEGAVLLCVAQGCPSPEYRWYTHNGPEPIPVSSGPRIRLLGPVLAIEAVTAEDGGVYKCSASNAGGEASADLRLTVSTPIHVEITPNVLSVHMGGTAEFRCLVTANGMSAGPQHITWFKDGRQLPSSGRVGDTLQVTGVTREDKGMYQCVVRRQEGDSFQASAELQLGDAPPSLVYSFIEQTLQPGPAVSLKCSAQGNPTPQIAWTLDGFPLPTNGRFMIGQYVTVHGDVISHVNISHVMVEDGGEYSCAAENRAGKTSHSARLNIYGLPYIRLIPKVTAVAGHTLYLKCPVAGYPIEEIHWERGGRELPEDMRQKVQSDGTLEIKEVQKSLDSGVYTCWARNKQGHSARRSGEVAVIVPPKLNPFHSSILSLNVGDRASITCSVIKGDIPLTITWRKDGRPIDPTQRMSVTQVDQYNSILLIENLTADHTGNYSCVVRNTAAETEGHQSLLVNVPPRWVVEPTDVSVERNRHIMLHCQAQGVPVPTILWKKATGSKSGDYEEVRERPYTKLLANGSLLLQHVKEDREGFYLCQANNGIGTGIGKVIQLKVNSSPYFAGQSKMVTVKKGDTAIMQCEVKGDKPINVVWLRNGKHELNPSTNYRVSIKQDATPEGISAEVQISNVDSSDSGAYFCQASNLYGRDQQLVQLQVQEPPQPPSSLEAATVSSRVVNLKWQPRGGDAAEVSKYIVEYREIDRQWQYIEISDPPAYSTIIENLKPATNYVFRIIAEGPAGRSSPSQELFIKTDPQRPAGPPLNLGARPISSTEILITWMPPSYELRHGEIQGYNIGYRSMHATTNSYNFTSISGDGEDGTGELLLGNLAKYTRYTIVAQAFNQVGPGPLSEPVTAQTMEDVPSKPPEDIRCMAQSSTSIQVSWQPPQAHHTNGLLQGYKVFYECATEDAISSGEMETRKTTSLTIHLSNLRKFSNYSIQVLAYTRMGDGVISPPSFCHTDEDAPEAPADIKIAISSPSSLYVSWLPPRDPNGIITKYNLYMRAVNGREELNNDKRNLQAHQHHFEAKQLQSHTEYQFWVSASTRVGEGKSSRVVSQLTSNRVPAKIVSFGGLTIRPWKSSASLNCMAVGQPRREWFKGDTLLRPGTQHNIQLLDSGEIIVTMLQTSDTGNYTCQVDNGIGTDRLTHNLLVQVPPGPPVLYVTSATSSSILLHWKIGSTGNAPITAFSLHYRRVHGNLEEMQLSRHASSHDLKGLFCGSTYQVYLVAHNKIGSSSASTTLNVKTQGQPPGIPPDSVLIAPNSTSVVLRLNGWPDNGCPIMYFVLQYRSVSTGLDDEWHLVSNALKPQRRFTLSGLLPSTMYRLKMEAHNVAGSTNAEYTFWTLTKDGDPPPPELVQRGHRSQIFYNNIQLLIPIIITITATIICIAFTVVCYKYRQRGRQRKDTMESQQTTDAQRDRYYATIHKVALQAGDKIPETSEDISPYATFQLSEASTLAQPHHSGPANTLLHSFMYHERAMTEGCASPPPAANSSKGRRRHSRKTEPESEESDSDPDQLTSSRTESSNQLDAKMKHSKDLDVAAGGPGDHGYNPNHYMRSIPGLIYHGTQSSTSSDLSPMSEQKSLPRRGRSRYHHLHLHNTNTTPRHKSSKAQSPIASPRSSCQNDNVPIQLPRMPSQFRPIHSSRTIGSTNSRLSPNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VMJ5</accession>
    <name>A0A6E8VMJ5_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">SAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VMJ5"/>
    <dbReference type="EnsemblMetazoa" id="ACON005305-RB">
        <property type="protein sequence ID" value="ACON005305-PB"/>
        <property type="gene ID" value="ACON005305"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_008250"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005305"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034355"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000380">
        <property type="term" value="P:alternative mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12884">
        <property type="entry name" value="SPRY_hnRNP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.30">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003034">
        <property type="entry name" value="SAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036361">
        <property type="entry name" value="SAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035778">
        <property type="entry name" value="SPRY_hnRNP_U"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12381:SF56">
        <property type="entry name" value="B30.2_SPRY DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12381">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN U FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13671">
        <property type="entry name" value="AAA_33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02037">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00513">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68906">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50800">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0488">Methylation</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAP" evidence="8">
        <location>
            <begin position="3"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="7">
        <location>
            <begin position="361"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="118"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="575"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="863"/>
            <end position="1051"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="57"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="152"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="175"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="235"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="299"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="315"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="575"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="593"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="877"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="925"/>
            <end position="934"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1028"/>
            <end position="1046"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005305-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1063" mass="117100" checksum="5A29B6280C5D15E6" modified="2020-08-12" version="1">MDLAKLKVADLKAELAARNLDTKGVKAVLLERLKEAIERETNAAATSVDPSGQQQYLAQQQQQHQQQQLLLQQQQLQAQQQQHAQQQLLAQQQQLQQQQQQQQQQNVAVAEQQPLPQEQTIEDLSMSSKRVDTPVRRRSTRRSMTRSPSPPNSSADNPHASLMGSARKRGRSRSMTKSPSPQRLTSEAPCLESVQEEEPEPVNAAPEQRLASVEQPAASTEPKMDESSSTAPVVEAPPAPSAQPEPAPATATAPSAMEVEESETDAPSAEPAKEENVAQAPVPAETTDGSAAEQAAPSETKDDEKKTTTEESAKPAESSEAAKPAAASSTGGETGSNSKSKAGPTKAEKKQSGQQQAQNNVVEFVSEEVEPQLSDEAEKTFTLSWYDSDLNLAIDDKDLLSAKPLSDGIFGLVWAGVRAGRGVTGGKVLYEVLVVDELQPATKSPLVPDGETPTAEMRIGWSTAMEGKRQLGETDHSFGYSSCGKKVVGGNFEEYGKPYGKGDVVGVYLDLDATPCCMQFTVNRVRQGNAFEFEKDSLDGTALFPHVYAKNLSFKVNFGTVEDGLPLVEKKVPAVEPATEKSGSEEASPNKDTTTDGEKASTEEAKVEGDDASKEESAPDNSAKKEAEKQDEDIKMEEAVKEAESADEQPAEKVPFDEAYTFLNRFMEENGDCVVEGLKGPSARDSCELVMMIGLPGSGKTTWVQNYLKENADTSFTLLSIDSLLENMKVEGKAREPSNTPQWQKVVEQLSRNMARLIEIACKRRRHILIDQTNVFASEQKRRLKGFGGFKTRRAIAVIPNLEEYKRRYEQKVAKYGAEVPETTLNTMKANIFVPTLEQKWYTEIVFAELPETEAQETLKKMNEEGRKLLPPRRNRANQANRQKHNNNNNNNNSNANSNYTSRWNHNRPQGGGGQQYGKNRYGNNSGGGGGYGNRYGNKSNDSVGQYGGGHHRGGAGDYRSGNGYGRRDDHHRGGNYGSGGNRYDSWNRNSGGGGYYNERGYSNRGYNQQQDHSTNRYDARRRDRRGYNNSSNWNTQGGQQQWNNSYQKSGNSDSWYLWWQDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VML5</accession>
    <name>A0A6E8VML5_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Serine-rich adhesin for platelets</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="EnsemblMetazoa" id="ACON005606-RA">
        <property type="protein sequence ID" value="ACON005606-PA"/>
        <property type="gene ID" value="ACON005606"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000472"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005606"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031912"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="278"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="468"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="723"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="810"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="860"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1017"/>
            <end position="1041"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1132"/>
            <end position="1202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1253"/>
            <end position="1278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1290"/>
            <end position="1324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1341"/>
            <end position="1404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="533"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="580"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="752"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="871"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="942"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1017"/>
            <end position="1036"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1132"/>
            <end position="1165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1167"/>
            <end position="1177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1264"/>
            <end position="1278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1296"/>
            <end position="1307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1310"/>
            <end position="1324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1350"/>
            <end position="1370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005606-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1404" mass="148789" checksum="DD7D6C68546E6526" modified="2023-06-28" version="2">MDVSESYSGDELIPESCLESEWIDEDEVFAILKQWETRGGGGVRGNSKAAAAATGYGNTAGSGHILLNQSFGNENNISVGSSIPGKLGQRFVDTGTFTRPKKRIETGIPNSPLAVDNNNTLSSSHTEFPSGGSPLLNVQIGGPVTPSSLATPSALASSTTGNRLLMQRSWLQDVSPPCSIMNSMEYSANEKITSSLITSGDFSSVGCLLNAGDDNANGSMPNVGNASYNGYNLYSVIEDRQRLENLCLDEESTLTKDCNISRTDLNAIESVSGVSTMQNSYESSGRTGALVGGKSSFEEESSPPPPPSTGAPAEEEQDAGKELINTTITTGVTAPNDTFELPEAEAERLLAGKLLLNGGGTYDARRPRNFNTYRKPRSSLNQTFEEVPHVDTDTTVVLPAAGQTFVAPKPVLDKHAVMNSTFGIVGDEQIGGAANGHGTFVRNRTLNNEERDDVRLINLTLDTVEPSITPPPAVPFDRTFKRRPRNGTFIGGEDEVRHLPFDSPLRPTVVGGGGAGPAAEPQLEDEAEPHGQRTPNGTYNQPQQLPVNSMLVQSTPFHGPSSRAKVAPELEDLSPIGPETQKTTPTSGLGRTMVRRSRNLEQDLRNITHEVVGTAEPAADMLLELETDDFDVQIRRLPSATATTGTGTGTDISNFLDAEKRISLQHFEEFEKSILESEHSGIDFDEMLNSLTADVRRSDEASDKLRQSLDNIKKRHSRINLEKQQQDEMKRKLHQQQQQPAEAQCDNKLADSLLSKSGGGSSSMCSSSGSERLLNRRSRYNEDVHLNLSPQQQQQQQQSPAMNATVIMANGGSEEGPAESADPVPTNSEVETYDRKNRDRFKTIRLGRRRDDSLGAVLPDVDQELAGPQPLLNGSGEDSGLSSSSSAFSRVDHNAAKMAGGPPSLANGGSRMAAGNGSNLPTMKTEPSGDDDEAVFKKPQAIQPSTMMQQPSGLTRPGESRLRSTLSKPRYYGAAGPLQGTIGGIQRKDLTLPLGNKSSSADCLEQREEDDQYLLQQPRLSGDKSLQQQQPTSRLATMSKLGGGSGGLLGTGRYSQFGLANKQLQLQQNQTVAGQPMGAPALKSPMGTKSKSYHSLTFAQANNSSGYGGSSGNLSKLHGSSALQLKLKHNSSNTELKSNTSKRFSLQQQAATNLRLASSSANQHQGAEGGGEGGRGAVVGRASLPLGRSPAAAQPNAARTGLNNSSTAAAMANKNALNTTTLSGGVQMRTASGLAKTSRLGLIRPSSGYFSYSTHRKHPDSDNESVNSLSSSSASSRGSLYRVDSQSIANGAQPPQFASATRTNSVEDVSGTSSSGNGLLTKLNGTGAGMGPGVLSAYASIEPPLPKNNPPVSSSMPAAPTTTGLKQPTAATGGAKPSGLRPPSAIRPPTVRSGLPRPTSYIRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VML6</accession>
    <name>A0A6E8VML6_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">N-terminal E2 ubiquitin-conjugating enzyme</fullName>
            <ecNumber evidence="2">2.3.2.23</ecNumber>
            <ecNumber evidence="6">2.3.2.25</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.</text>
            <dbReference type="EC" id="2.3.2.23"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-N-terminal-amino acid = [E1 ubiquitin-activating enzyme]-L-cysteine + N-terminal-ubiquitinyl-[acceptor protein].</text>
            <dbReference type="EC" id="2.3.2.25"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="2"/>
    <dbReference type="EC" id="2.3.2.25" evidence="6"/>
    <dbReference type="RefSeq" id="XP_040222566.1">
        <property type="nucleotide sequence ID" value="XM_040366632.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VML6"/>
    <dbReference type="EnsemblMetazoa" id="ACON005324-RA">
        <property type="protein sequence ID" value="ACON005324-PA"/>
        <property type="gene ID" value="ACON005324"/>
    </dbReference>
    <dbReference type="GeneID" id="120949368"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_014216"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005324"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040031"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd23808">
        <property type="entry name" value="UBCc_UBE2W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000022">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="8">
        <location>
            <begin position="42"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00042168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005324-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="190" mass="21886" checksum="171F3F2300CF00A1" modified="2020-08-12" version="1">MFNRLRMRKEKPSKSETISTTNTTVIQDAAKDNKKLILDNSRWERRLQKELMSLIKEPPPGVSVDEESVSQNLTQWIINIDGVEGTLYEGEHFQLLFKFNNKYPFDSPEVTFIGSNIPIHPHVYSNGHICLSILTDDWSPALSVQSVCLSISSMLSSCREKRRPPDNGIYVKTCNKNPKKTKWWYHDDSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VP16</accession>
    <name>A0A6E8VP16_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">phenylalanine 4-monooxygenase</fullName>
            <ecNumber evidence="5">1.14.16.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Phe-4-monooxygenase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(6R)-L-erythro-5,6,7,8-tetrahydrobiopterin + L-phenylalanine + O2 = (4aS,6R)-4a-hydroxy-L-erythro-5,6,7,8-tetrahydrobiopterin + L-tyrosine</text>
            <dbReference type="Rhea" id="RHEA:20273"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15642"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
            <dbReference type="ChEBI" id="CHEBI:59560"/>
            <dbReference type="EC" id="1.14.16.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 13">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 1/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.</text>
    </comment>
    <dbReference type="EC" id="1.14.16.1" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8VP16"/>
    <dbReference type="EnsemblMetazoa" id="ACON005712-RC">
        <property type="protein sequence ID" value="ACON005712-PC"/>
        <property type="gene ID" value="ACON005712"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010383"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005712"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_036069"/>
    <dbReference type="UniPathway" id="UPA00139">
        <property type="reaction ID" value="UER00337"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004505">
        <property type="term" value="F:phenylalanine 4-monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048066">
        <property type="term" value="P:developmental pigmentation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042416">
        <property type="term" value="P:dopamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006559">
        <property type="term" value="P:L-phenylalanine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd04904">
        <property type="entry name" value="ACT_AAAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03347">
        <property type="entry name" value="eu_PheOH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.800.10:FF:000004">
        <property type="entry name" value="Tyrosine 3-monooxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.800.10">
        <property type="entry name" value="Aromatic amino acid hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001273">
        <property type="entry name" value="ArAA_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036951">
        <property type="entry name" value="ArAA_hydroxylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036329">
        <property type="entry name" value="Aro-AA_hydroxylase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019774">
        <property type="entry name" value="Aromatic-AA_hydroxylase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041912">
        <property type="entry name" value="Euk_PheOH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005961">
        <property type="entry name" value="Phe-4-hydroxylase_tetra"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019773">
        <property type="entry name" value="Tyrosine_3-monooxygenase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01268">
        <property type="entry name" value="Phe4hydrox_tetr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11473">
        <property type="entry name" value="AROMATIC AMINO ACID HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11473:SF24">
        <property type="entry name" value="PHENYLALANINE-4-HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01842">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00351">
        <property type="entry name" value="Biopterin_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000336">
        <property type="entry name" value="TH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00372">
        <property type="entry name" value="FYWHYDRXLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56534">
        <property type="entry name" value="Aromatic aminoacid monoxygenases, catalytic and oligomerization domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51410">
        <property type="entry name" value="BH4_AAA_HYDROXYL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0408">Iron</keyword>
    <keyword evidence="6 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-0585">Phenylalanine catabolism</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ACT" evidence="15">
        <location>
            <begin position="14"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="Biopterin-dependent aromatic amino acid hydroxylase family profile" evidence="14">
        <location>
            <begin position="83"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="262"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="267"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="307"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000336-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR601273-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005712-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="428" mass="49371" checksum="4DE921770A97F049" modified="2020-08-12" version="1">MEGHDAAEAKNVCLIFSPEQEEAGALAKMLRIFDDHRVNLLHIESRSSTRGPGYEFMVECDGKRGTLGPAIEAIREKSNYFNIISRDYKDNEATVPWFPRRIRDLDRFANQILSYGAELDSDHPGFTDQTYRERRKYFADIAFNYKHGQPLPHVDYTPEEVNTWREVFRNLTKLYRTHACREHNHVFPLLIENCGYREDNIPQLEDVSNFLKDCTGFTLRPVAGLLSSRDFLAGLAFRVFHSTQYIRHPSKPLYTPEPDVCHELLGHAPLFADPSFAQFSQEIGLASLGAPDEFVEKLATCFWFTVEYGMCRQNGELKAYGAGLLSSFGELQYCLTDKPELREFDPAKTCEQKYPITEYQPVYFVSESFEDAIQKMINYANTIPRPFGVRYDPYTQSIEILDSVPQISNLMRNIHNEFEVLQNAFKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VP23</accession>
    <name>A0A6E8VP23_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the troponin C family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040224749.1">
        <property type="nucleotide sequence ID" value="XM_040368815.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VP23"/>
    <dbReference type="SMR" id="A0A6E8VP23"/>
    <dbReference type="EnsemblMetazoa" id="ACON006179-RB">
        <property type="protein sequence ID" value="ACON006179-PB"/>
        <property type="gene ID" value="ACON006179"/>
    </dbReference>
    <dbReference type="GeneID" id="120950626"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012938"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON006179"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033198"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000336">
        <property type="entry name" value="HLH domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000103">
        <property type="entry name" value="Troponin C Ib"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF0">
        <property type="entry name" value="CALMODULIN LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="7"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="43"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="83"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="119"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON006179-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="153" mass="17324" checksum="F775C27E80B30839" modified="2020-08-12" version="1">MDDLDKAQLELLRNAFNAFDQEKKGCIGTQMVGTILSMLGHQLDDKMLKEIIDEVDADGSGELEFEEFVTLAARFMVEEDAEAMQQELKEAFRLYDKEGNGYITTQVLREILKELDDNLTNEDLDMMIEEIDSDGSGTVDFDEFMEVMTGGDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VPD3</accession>
    <name>A0A6E8VPD3_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Alpha-tubulin N-acetyltransferase</fullName>
            <shortName evidence="5">Alpha-TAT</shortName>
            <shortName evidence="5">TAT</shortName>
            <ecNumber evidence="4 5">2.3.1.108</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Acetyltransferase mec-17 homolog</fullName>
        </alternativeName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Specifically acetylates 'Lys-40' in alpha-tubulin on the lumenal side of microtubules. Promotes microtubule destabilization and accelerates microtubule dynamics; this activity may be independent of acetylation activity. Acetylates alpha-tubulin with a slow enzymatic rate, due to a catalytic site that is not optimized for acetyl transfer. Enters the microtubule through each end and diffuses quickly throughout the lumen of microtubules. Acetylates only long/old microtubules because of its slow acetylation rate since it does not have time to act on dynamically unstable microtubules before the enzyme is released.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 5">
            <text>L-lysyl-[alpha-tubulin] + acetyl-CoA = N(6)-acetyl-L-lysyl-[alpha-tubulin] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15277"/>
            <dbReference type="Rhea" id="RHEA-COMP:11278"/>
            <dbReference type="Rhea" id="RHEA-COMP:11279"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.108"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the acetyltransferase ATAT1 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.108" evidence="4 5"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8VPD3"/>
    <dbReference type="EnsemblMetazoa" id="ACON005828-RB">
        <property type="protein sequence ID" value="ACON005828-PB"/>
        <property type="gene ID" value="ACON005828"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002467"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005828"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_029974"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019799">
        <property type="term" value="F:tubulin N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048666">
        <property type="term" value="P:neuron development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070507">
        <property type="term" value="P:regulation of microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000060">
        <property type="entry name" value="Alpha-tubulin N-acetyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03130">
        <property type="entry name" value="mec17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038746">
        <property type="entry name" value="Atat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007965">
        <property type="entry name" value="GNAT_ATAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12327">
        <property type="entry name" value="ALPHA-TUBULIN N-ACETYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12327:SF0">
        <property type="entry name" value="ALPHA-TUBULIN N-ACETYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05301">
        <property type="entry name" value="Acetyltransf_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51730">
        <property type="entry name" value="GNAT_ATAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="6">
        <location>
            <begin position="1"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="120"/>
            <end position="133"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="156"/>
            <end position="165"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="site" description="Crucial for catalytic activity" evidence="5">
        <location>
            <position position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00051998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00066570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005828-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="302" mass="33861" checksum="E33AB3ACCF74E9EC" modified="2020-08-12" version="1">MEFRFNMHPLFRARIVRINNSLLPTGFVAQSRRVALDATAQISEIINTIGSMSAQAQGLSVPVTTAQKLRNSDHHIYLMFESNDRNGLVVGILKVGRKSLYVFDPSGETVNVTAPCVLDFYVHESRQRGGLGRELFEHMLREENIQPDELAIDRPSEKLLGFLQKHYGLSKKIPQMNNFVVYEGFFASKAQNSTDIDGRRMHITASPNTNLFGPTFTTTEERRRSTSQTRTNVAPMPIIAQPPVGRYAAKRPTCSMAQVNHYSSMVGKIRFPEENTGNGKRSVFIIHNSPTTVSTEPNSNFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VPD6</accession>
    <name>A0A6E8VPD6_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="6">PDZ domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VPD6"/>
    <dbReference type="EnsemblMetazoa" id="ACON006260-RC">
        <property type="protein sequence ID" value="ACON006260-PC"/>
        <property type="gene ID" value="ACON006260"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004821"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON006260"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032404"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031941">
        <property type="term" value="C:filamentous actin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001725">
        <property type="term" value="C:stress fiber"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051371">
        <property type="term" value="F:muscle alpha-actinin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061061">
        <property type="term" value="P:muscle structure development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23068">
        <property type="entry name" value="PDZ_ZASP52-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000192">
        <property type="entry name" value="Uncharacterized protein, isoform J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050604">
        <property type="entry name" value="PDZ-LIM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24214">
        <property type="entry name" value="PDZ AND LIM DOMAIN PROTEIN ZASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24214:SF55">
        <property type="entry name" value="Z BAND ALTERNATIVELY SPLICED PDZ-MOTIF PROTEIN 66, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0440">LIM domain</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PDZ" evidence="5">
        <location>
            <begin position="9"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="99"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="173"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="222"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON006260-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="266" mass="29337" checksum="0197DD24017368EA" modified="2020-08-12" version="1">MSPKPHDFTVTLRRSSPQVAWGIRLAGGTDLNAPLIITRVQDHSPAQPELLRGDVITKIDQYDARDLMHIDAQNLFRNAGNQIKVTVRRDDKVALHQSAHPINGNPVAPLSPANFAQPYQPYAEPGQPAKPQLHQNFPPPDPTQLLPSTATSLPHGPQSYAAALERPVETLPHTVFPGVDGTGAYHLPKQPYPPPAPTSLNDANEAITNQPYRTTPLVLPGAKVPKKDTLPTESYLRHHPNPAMRAPPAHDYTDSLMKQKVQQYWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VQU0</accession>
    <name>A0A6E8VQU0_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VQU0"/>
    <dbReference type="EnsemblMetazoa" id="ACON006347-RB">
        <property type="protein sequence ID" value="ACON006347-PB"/>
        <property type="gene ID" value="ACON006347"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004614"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON006347"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035010"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000017">
        <property type="entry name" value="potassium voltage-gated channel subfamily KQT member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000016">
        <property type="entry name" value="Putative potassium voltage-gated channel subfamily KQT member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1910">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003937">
        <property type="entry name" value="K_chnl_volt-dep_KCNQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013821">
        <property type="entry name" value="K_chnl_volt-dep_KCNQ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47735">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL SUBFAMILY KQT MEMBER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47735:SF9">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL SUBFAMILY KQT MEMBER 4-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03520">
        <property type="entry name" value="KCNQ_channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00169">
        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01459">
        <property type="entry name" value="KCNQCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0630">Potassium</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="218"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="246"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="292"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="357"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="418"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="15">
        <location>
            <begin position="218"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel voltage dependent KCNQ C-terminal" evidence="16">
        <location>
            <begin position="564"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="519"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="685"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="919"/>
            <end position="971"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="49"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="70"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="114"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="706"/>
            <end position="719"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF03520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON006347-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="971" mass="108049" checksum="96275C0B5E950A88" modified="2020-08-12" version="1">MRSTAMEDVYENEPHAPDPAEQPKPFNGSSGKNVSLRILSMPSSPLPPVAGAVPSSPSAVSLSSTNARDTRHRSSKDRVSRKARVSTIASPGASGRTDLTPGVTTIDEGGASDGGNTPSSLAPCSSTVKKSKVDFVRFDSTEIHYTENEEVHKKISDEIKSACKLSDKNRADRLARPRMSLLGKPLNYNRGSRRDARYRRLQSRVYNFLERPRGFKAIFYHVCVFLMVFTCLALSVFSTIQEYEDQAIAILFVMEIIVVIWFSIEFFLRLWSSGCRSRYQGAVGRLKFLKRPFCIIDIVTIAASIVVLVMGTSGQVFATSALRGLRFFQILRMVRMDRRGGTWKLLGSVVYAHRQELITTLYIGFLGLIFASFLVYLMEKDVRGTKFSNFAQALWWGVITLCTVGYGDMVPETWQGKIIASFCALLGISFFALPAGILGSGFALKVQQQQRQKHMIRRRQPAATLIQSLWRCYAADEHSMSEATWKIHQVPLPSPPPSRASSSFKHNASFVARLPTIRRHKSQSLHSPGNPKPPSGGSGRTGRCPRVTDINASSENLDEDEEPRCVQLTNQHKGAIRFIRKLKYFVARKKFREAQKPYDVKDVMEQYTSGHADLLTRVRNLQFRLDQILGKQGSKSKDVYASKISLASRVVKIERQVDDIETKVDSFIDLYMQDRMRLLSLPLHPDTSHSNNPNLPSLPPKGGGASAVITSVTTSSSGSLKPKPILVDKQFSEPNSPIAKTFEEQPLQQKRPPMQRGFSDLGHRIKKRVTLSSIPPQYVGNSTGQPSDRGDVVIVVPNIDAEHLEPVGIEELASVCIDTEIEIEPGSPKTITESSIIMMDEEDDEEDEEEDIEEEDLDIGAEIDPDSPPWDVYGDMDVEDQDDTTENTALLRTAAKTEIIVTPISPVSSAFELNRMDSEEYRRSKQVAANNNEGEQHQQQQQQQQMLAITGPSGAMDSLKPERASNGSDDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VR34</accession>
    <name>A0A6E8VR34_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Cyclic nucleotide-binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cAMP-dependent kinase regulatory chain family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040218583.1">
        <property type="nucleotide sequence ID" value="XM_040362649.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VR34"/>
    <dbReference type="EnsemblMetazoa" id="ACON006448-RA">
        <property type="protein sequence ID" value="ACON006448-PA"/>
        <property type="gene ID" value="ACON006448"/>
    </dbReference>
    <dbReference type="GeneID" id="120947380"/>
    <dbReference type="KEGG" id="acoz:120947380"/>
    <dbReference type="CTD" id="40305"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_008284"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON006448"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042399"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030552">
        <property type="term" value="F:cAMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004862">
        <property type="term" value="F:cAMP-dependent protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034236">
        <property type="term" value="F:protein kinase A catalytic subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007611">
        <property type="term" value="P:learning or memory"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd12097">
        <property type="entry name" value="DD_RI_PKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000013">
        <property type="entry name" value="cAMP-dependent protein kinase type I regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000006">
        <property type="entry name" value="cAMP-dependent protein kinase type I-alpha regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.890.10">
        <property type="entry name" value="cAMP-dependent protein kinase regulatory subunit, dimerization-anchoring domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050503">
        <property type="entry name" value="cAMP-dep_PK_reg_su-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012198">
        <property type="entry name" value="cAMP_dep_PK_reg_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003117">
        <property type="entry name" value="cAMP_dep_PK_reg_su_I/II_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE REGULATORY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635:SF152">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE TYPE I REGULATORY SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02197">
        <property type="entry name" value="RIIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000548">
        <property type="entry name" value="PK_regulatory"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00103">
        <property type="entry name" value="CAMPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00394">
        <property type="entry name" value="RIIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47391">
        <property type="entry name" value="Dimerization-anchoring domain of cAMP-dependent PK regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00888">
        <property type="entry name" value="CNMP_BINDING_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00889">
        <property type="entry name" value="CNMP_BINDING_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0114">cAMP</keyword>
    <keyword evidence="3 7" id="KW-0116">cAMP-binding</keyword>
    <keyword evidence="5 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="9">
        <location>
            <begin position="129"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="9">
        <location>
            <begin position="247"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="60"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>3',5'-cyclic AMP</name>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>3',5'-cyclic AMP</name>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="318"/>
        </location>
        <ligand>
            <name>3',5'-cyclic AMP</name>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="327"/>
        </location>
        <ligand>
            <name>3',5'-cyclic AMP</name>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000548-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON006448-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="373" mass="41999" checksum="B4B5CD1F4DB71BC6" modified="2020-08-12" version="1">MSNAQEEEQHLRECESYIQTHCIQRLLKDCIVQLCVSRPENPIVFLRQYFQKLEREQAKLDASKQAVTPDDLDDLSPMPQTAVPPVRRRGGISAEPVTEEDATSYVKKVVPKDYKTMAALSKAIAKNVLFSHLDENERSDIFDAMFPCNFLPGEPIIQQGDEGDNFYVIDIGEVEVFVNNEQVTTISEGGSFGELALIYGTPRAATVRAKTDVKLWGIDRDSYRRILMGSTIRKRKMYEEFLSRVSILESLDKWERLTVADALEPVSFEDGETIVRQGEPGNDFYIIVEGCATVMQKRGDSEEAAEVGRLGPSDYFGEIALLLDRPRAATVIARGPLKCVKLDRARFERVLGLCADILKRNITQYNSFVSLSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VR55</accession>
    <name>A0A6E8VR55_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DEP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="EnsemblMetazoa" id="ACON007010-RA">
        <property type="protein sequence ID" value="ACON007010-PA"/>
        <property type="gene ID" value="ACON007010"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004583"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007010"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_043233"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990130">
        <property type="term" value="C:GATOR1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904262">
        <property type="term" value="P:negative regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010508">
        <property type="term" value="P:positive regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04449">
        <property type="entry name" value="DEP_DEPDC5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045838">
        <property type="entry name" value="DEPDC5_CTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027244">
        <property type="entry name" value="IML1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055213">
        <property type="entry name" value="IML1_double_psi_beta_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048255">
        <property type="entry name" value="IML1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13179">
        <property type="entry name" value="DEP DOMAIN CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13179:SF8">
        <property type="entry name" value="GATOR COMPLEX PROTEIN DEPDC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19418">
        <property type="entry name" value="DEPDC5_CTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12257">
        <property type="entry name" value="IML1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23013">
        <property type="entry name" value="IML1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IML1 N-terminal double psi beta-barrel" evidence="4">
        <location>
            <begin position="14"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="Vacuolar membrane-associated protein Iml1 N-terminal" evidence="2">
        <location>
            <begin position="112"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="domain" description="DEPDC5 C-terminal" evidence="3">
        <location>
            <begin position="1258"/>
            <end position="1587"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="444"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="561"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1022"/>
            <end position="1108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="561"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1022"/>
            <end position="1042"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1084"/>
            <end position="1099"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF19418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007010-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1609" mass="182078" checksum="524E73F284E3F3F7" modified="2020-08-12" version="1">VHTTVLLCAKMMQKLFKLVTHQKTFSSKYHSEDLVLNPKEHPDLKLNDIVEIYLPEDEGCRLLLQVTCLNKDFNSRETISVEVNVATTFNLKAYNDVYMRVVDPADVALDSVEITFKDQYIGRSEMWRLKTRLTNTCVYLNKKIESCEGCIRCQVYEMWSQGERVACGVINEDTKVVFRSNTSMVYLFLQMSSEMWDFDIYGDLYFEKAVNGFLADLYKKWQKLGSNHEVTIVLFSRTFYSAKSLDEFPPHMRDCLQIDYRGRFYEDFYRVAIQNERCDDWSTTLVQLRRLFTGYRDVVLKYHERTDLPAGVRIPPATNSTAAQGNFLEVLNISLNVFEKHYLDRSFDRTGQLSVVITPGVGVFEVDRELTNITKQRIIDNGVGSDLVCVGEQPLHAVPLLKFHNKDTLTSIDDYSMPHWINLSFYSTNKKLPYSTFIPRIKPPPAIGGSTEPQQARPPGTGRLRLSKQAADKKNEYIHNCLFDYDAYDDQIFQLPAVHGTSSLQRIARTKKTSVPSLDGFGTYARACEWEQDTLSPSRLRRKMSDPDIHHASNSLLNVSVASRSSSPNTSDILGMPKPTAITSPIMEGRTGAFGSSSGPVVRTGRALINPFDPSHVTIKLTSNRRRWTHIFPKGPQGVLIQQHHYQAVPAANPTPSYFVDLDSSSEYAHTSTCTWGRARQGSTASFDSRLNEDDLKWISENGCNRKKSVLHSPAGPHVPVTPSKSLTLLWGATGEQEWTPALTTVGRVIIGVDWKSLTMPACLPITTDYFPDKRSLHNDYVVSDYTLLPEDFNADYAQNRAVYKKPLSTDEVFKELVSQRLAQGFQLIVLPDKSVTNPPHAPCCGGHLQTPSYFSSASSVLRRQPPQEATKEYLLSIGRIFHRITLSGSAITVTRYRPRHPYPPINVDYRYRFHAPQHDTYEVSGVHFTTEKLENFNWNYMDQYICTRGDTDYPLHENMKYWRYRMYLLPKDDPAMKKILDGTVRRCDIYHEDSPFPPERQICNDFVRFVEAHLNKIKKLQPAKKARGCPHQTHLTRRRHSTSILSRPPPNQVILTRTGSKVLDKSGSAVQAPSPVGTGAIGTGTATSNGTTHATGNGPATPNTPGIPSPMMMNVSSVGGRTTVDGIPLSTLNTAGTEQGLLEDGDDFGGEGGKLKPTATLQEIFDALRHPAHGVGFLAPAQSMPSCTFVSYDAINWLQNRIEGPCKPVEILEEMRRNRLICHASGDFNKPVLPGFCLYYIVRQDRDSPEYVPPLGDLAAFENEWMEVEIRHPDLTRPVDPHDRGVPTFLRESIARPDEDDDDGQLLYKQTHLEIDVGSKSDRTEWGHARYHQRMVPGHAYEIVVQWITASGPIVYDLIYGWCRKAQPCGFQLVTFPADPLAEPFTEKSDPLRGPIFVPLDIDCLKQHRSCLFQEFPKETWPTRLLLFQEAIVRRFGFMRCTVETKTGSNQVSLDHQYVHCSGNMFLQVPNPNVGGMRSTRQRLASGGSNFKKPIAFINRYSASYESQMVAAPSGHEPSYITRHVTGAKGSAKDEGGEVMRRTGFLWSWNHMIPNKKWKSLVIAQTDDLFQLKMLRDFKEFCANADQRLVSFWESCWELKEKASLERT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VR56</accession>
    <name>A0A6E8VR56_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">ADP-ribosylation factor GTPase-activating protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">ADP-ribosylation factor 1 GTPase-activating protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">ARF1-directed GTPase-activating protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">GTPase-activating protein (GAP) for the ADP ribosylation factor 1 (ARF1). Involved in membrane trafficking and /or vesicle transport. Promotes hydrolysis of the ARF1-bound GTP and thus, is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles, a prerequisite for vesicle's fusion with target compartment. Probably regulates ARF1-mediated transport via its interaction with the KDELR proteins and TMED2. Overexpression induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, as when ARF1 is deactivated. Its activity is stimulated by phosphoinosides and inhibited by phosphatidylcholine.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_040229241.2">
        <property type="nucleotide sequence ID" value="XM_040373307.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VR56"/>
    <dbReference type="EnsemblMetazoa" id="ACON006462-RB">
        <property type="protein sequence ID" value="ACON006462-PB"/>
        <property type="gene ID" value="ACON006462"/>
    </dbReference>
    <dbReference type="GeneID" id="120953410"/>
    <dbReference type="CTD" id="55738"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_003498"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON006462"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035303"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032012">
        <property type="term" value="P:regulation of ARF protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030100">
        <property type="term" value="P:regulation of endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08830">
        <property type="entry name" value="ArfGap_ArfGap1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.150:FF:000008">
        <property type="entry name" value="ADP-ribosylation factor GTPase activating protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46395">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR GTPASE-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46395:SF1">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR GTPASE-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00405">
        <property type="entry name" value="REVINTRACTNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00105">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50115">
        <property type="entry name" value="ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="4" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 18" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Arf-GAP" evidence="20">
        <location>
            <begin position="7"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="136"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="205"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="294"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="455"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="19">
        <location>
            <begin position="136"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="150"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="19">
        <location>
            <begin position="160"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="176"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="306"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="324"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="347"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="384"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="404"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="416"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="460"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00058112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00071258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00077418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00081514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON006462-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="482" mass="51535" checksum="FABAA61CE9F8E8E5" modified="2020-08-12" version="1">MASPRTRRVLSELKPSDGNNKCFECGTHNPQWVSVTYGIWICLECSGKHRGLGVHLSFVRSVSMDKWKDIELEKMKVGGNRKAREFLDAQDDWDETMPIQRKYSTRAAALYRDRISTLAQGQPWDESSALARVNGSGGYAASGSSGGSTGSMTHSRSSGSMGGGSGAGGNGGYQNGGDNYYQDGGSYQQYQTPEFKAQKEDFFSRKQEENAARPENLPPNQGGKYAGFGYTMDPPPRSQSHELFDTVQSSLATGWNVFSKVANVAKENALKYGSIASQKVTEVGRKGWGGFGGPGSGSYSTPSGSNDQYSNISSDSGVSSPINGRQPGGYQQQRGSTGGSGRRDESSWSNNGWSNDSNSNSLPQSYQNGDNDDDGWNGFESYSGPASSGQPTSASAELMANVDMRNENGPDRHASSKSGSTRRTSNGSANRQHSAKASGAAAGIDEDFAALDIKSNRAPKSSSTSKKANNNPEDDLWNMLNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VSV9</accession>
    <name>A0A6E8VSV9_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Sodium channel protein Nach</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VSV9"/>
    <dbReference type="EnsemblMetazoa" id="ACON007084-RB">
        <property type="protein sequence ID" value="ACON007084-PB"/>
        <property type="gene ID" value="ACON007084"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002398"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007084"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038152"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015280">
        <property type="term" value="F:ligand-gated sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.820">
        <property type="entry name" value="Acid-sensing ion channel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001873">
        <property type="entry name" value="ENaC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11690">
        <property type="entry name" value="AMILORIDE-SENSITIVE SODIUM CHANNEL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11690:SF247">
        <property type="entry name" value="PICKPOCKET 23, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00858">
        <property type="entry name" value="ASC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="4 12" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="10 12" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="37"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007084-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="472" mass="54926" checksum="82F79B6F1F970372" modified="2020-08-12" version="1">MVYTFSFTKAIQNVLAQTSLHGVFHLVHKRSTYVEKVIWLGIISMALFAGCYNVGSFWVRYWTNPTVIALDRDYHLWNTTFPSLTVCFQKRLNEAARDQLIAQVDPELAERYGEFLDTLLESDIENVGHLAEFDEFEGTDLRQLLNELTDRPNAVVTMEGDLHGTLVRSLTEMGICYTFNTAIGRYLSIDPYAPDEKLYEVSLFGEMFQVCRRSLVKSIFGKIFFHSPYEMPTIRKSLLIERGFFTFTKMDFNALAITSVPALKSLSIKQRKCRFPHESNLKFFPEYYSYGLCLLECKFYLFLKHCDCIPYFYQISDPSKYCKLSQLACVELYQSYISFLTADELREIFGACNCLKNCDDVTFTLQHYGSTFWFNDPVIKWSIRIPKIRYSRRIIFDFIDAMVSTGSILEFFFGFSIITLVELAYFSMRNLILSCLRIKMITRICKRKQRKVKNGIIGNQTIIKKVPTIRVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VT70</accession>
    <name>A0A6E8VT70_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="6">SERPIN domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the serpin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VT70"/>
    <dbReference type="EnsemblMetazoa" id="ACON007691-RC">
        <property type="protein sequence ID" value="ACON007691-PC"/>
        <property type="gene ID" value="ACON007691"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010250"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007691"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037348"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.497.10:FF:000062">
        <property type="entry name" value="AGAP007691-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.497.10">
        <property type="entry name" value="Antithrombin, subunit I, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023796">
        <property type="entry name" value="Serpin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000215">
        <property type="entry name" value="Serpin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036186">
        <property type="entry name" value="Serpin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042178">
        <property type="entry name" value="Serpin_sf_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461:SF211">
        <property type="entry name" value="GH10112P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461">
        <property type="entry name" value="SERINE PROTEASE INHIBITOR, SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00079">
        <property type="entry name" value="Serpin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00093">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56574">
        <property type="entry name" value="Serpins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0722">Serine protease inhibitor</keyword>
    <feature type="domain" description="Serpin" evidence="5">
        <location>
            <begin position="1"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007691-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="331" mass="37348" checksum="81A269AA0F6732DD" modified="2020-08-12" version="1">MFYPLAGAAVQEDFQVAFGLPEDVHAAIEQQQRLAQQLHDGQHLKALSFVLVEETLRLDSEFERLFHRTFQTTVEPVDLTDDIPSALAVNSFYQRANTEIEDFIGEGDVFSLPPCHKLMLFSGVSVLTPLAIRFNPADTALELFQFINAPTQRVSTMHTTAFVRRCLHNELRCKVVDIPFDAASGLSMLVLLPYDGTELRQIVNSITPAHLAQIDERLQSCWTDLKLPKFFVREKTDPKQTLGKLGYGGVFEIDDLHVFHDSGRTRLNGFIQHCYLAVSESGSGIPAPPDTPSEFEFHGNRPFMFLIRRTMDGNVLQVGNFSKYIDPDEQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VT76</accession>
    <name>A0A6E8VT76_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Titin</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Myofibril</location>
            <location evidence="1">Sarcomere</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON007563-RB">
        <property type="protein sequence ID" value="ACON007563-PB"/>
        <property type="gene ID" value="ACON007563"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_013821"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007563"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039286"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031674">
        <property type="term" value="C:I band"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040017">
        <property type="term" value="P:positive regulation of locomotion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060298">
        <property type="term" value="P:positive regulation of sarcomere organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045989">
        <property type="term" value="P:positive regulation of striated muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000147">
        <property type="entry name" value="Myosin light chain kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000425">
        <property type="entry name" value="Myosin light chain kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000080">
        <property type="entry name" value="Myosin light chain kinase, smooth muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000107">
        <property type="entry name" value="Myosin, light chain kinase a"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000032">
        <property type="entry name" value="palladin isoform X1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000119">
        <property type="entry name" value="Sallimus, isoform P"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000430">
        <property type="entry name" value="Sallimus, isoform P"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000966">
        <property type="entry name" value="Sallimus, isoform P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001128">
        <property type="entry name" value="Sallimus, isoform P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001196">
        <property type="entry name" value="Sallimus, isoform P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001269">
        <property type="entry name" value="Sallimus, isoform P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000809">
        <property type="entry name" value="Sallimus, isoform Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001163">
        <property type="entry name" value="Sallimus, isoform Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001403">
        <property type="entry name" value="Sallimus, isoform U"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000697">
        <property type="entry name" value="titin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000962">
        <property type="entry name" value="titin isoform X1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47633">
        <property type="entry name" value="IMMUNOGLOBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47633:SF4">
        <property type="entry name" value="MYOPALLADIN ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="41"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="39"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="36"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="41"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="40"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="10" id="KW-0514">Muscle protein</keyword>
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            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
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                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0769">Symport</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="140"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="179"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="208"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="310"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="347"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="372"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="402"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="437"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="18"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007732-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="500" mass="54951" checksum="650BC724748A922E" modified="2020-08-12" version="1">MYSSSWQQRVSRFFIIPQRVILAIMGFLAIMNAYTMRISLSIAITEMVNKTHGATEEDGGVCPIDESANPDDFTGGEFDWDEELQGIILSSFYWGYVITHIPGGMLAEKFGGKWTLSLGILSTAFFTLITPWAVELGGSTALIVIRVMMGLGEGTTFPALSALMATWIPAKERSKLGSLVFGGGQVGTILGNLLSGVLLHNIEGWSSVFYFFGGLGVLWFVIFTLLCYSDPESHPFISEKEKAYLKQELGTLERDRTLPPTPWRYILTSVPMMALVCAQIGHDWGFFIMVTDLPKYMSDVLRFSIKDNGLYSSLPYLVMWIVSLSTGVLSDWLITSGRMTITFGRKLFTTIASIGPACFIVGASYAGCEKAVVVMLFTFAMGLMGTFYPGMKVNPLDLSPNYAGTLMAITNGIGAITGIIAPYVVGVMTPNHTLEEWRIVFWISFAIFNVTNLAYIIWASGEVQPWNTPHLMNKSVEAGESIDVPANAKKSVQSLEAIKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VT82</accession>
    <name>A0A6E8VT82_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Tetraspanin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the tetraspanin (TM4SF) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040218836.1">
        <property type="nucleotide sequence ID" value="XM_040362902.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VT82"/>
    <dbReference type="EnsemblMetazoa" id="ACON007209-RA">
        <property type="protein sequence ID" value="ACON007209-PA"/>
        <property type="gene ID" value="ACON007209"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON007209-RB">
        <property type="protein sequence ID" value="ACON007209-PB"/>
        <property type="gene ID" value="ACON007209"/>
    </dbReference>
    <dbReference type="GeneID" id="120947520"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010707"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007209"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031378"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03127">
        <property type="entry name" value="tetraspanin_LEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1450.10">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000301">
        <property type="entry name" value="Tetraspanin_animals"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008952">
        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF521">
        <property type="entry name" value="IP01817P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002419">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00259">
        <property type="entry name" value="TMFOUR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48652">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="56"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="194"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="145"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="146"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR002419-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007209-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="228" mass="24588" checksum="43955EA721A66B8B" modified="2020-08-12" version="1">MALNCGHSTIKYLLFIFNLLCSLCGIALIVIGAVSLVKLEELREISDEYNIVAPSILLIVFGSVVFVISFFGCCGAIRESYCMTSTYSFFLILLVIAQIVIAVLVFVFVGDVTTAIKKGFERVFENRDNPVNADLVNSIQQNLQCCGKRSFLDYGVASFPQSCCSSGGTAPLCVPFASGCLSRLSEFLDSAGNIVAWVSLGVAAVELVGLISACCLANAIRNQQRRYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VTL1</accession>
    <name>A0A6E8VTL1_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Probable U2 small nuclear ribonucleoprotein A'</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the U2 small nuclear ribonucleoprotein A family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040224036.1">
        <property type="nucleotide sequence ID" value="XM_040368102.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VTL1"/>
    <dbReference type="EnsemblMetazoa" id="ACON007670-RA">
        <property type="protein sequence ID" value="ACON007670-PA"/>
        <property type="gene ID" value="ACON007670"/>
    </dbReference>
    <dbReference type="GeneID" id="120950234"/>
    <dbReference type="CTD" id="35713"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001397"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007670"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_036825"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030620">
        <property type="term" value="F:U2 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000026">
        <property type="entry name" value="U2 small nuclear ribonucleoprotein A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044640">
        <property type="entry name" value="RU2A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10552">
        <property type="entry name" value="U2 SMALL NUCLEAR RIBONUCLEOPROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10552:SF6">
        <property type="entry name" value="U2 SMALL NUCLEAR RIBONUCLEOPROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14580">
        <property type="entry name" value="LRR_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="183"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00069881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007670-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="247" mass="28096" checksum="08DDB89220F80287" modified="2020-08-12" version="1">MVKLTPDLINQSMQYMNPCRDRELDLRGYKIPQIENMGATLDQYDTIDFSDNDIRKLDGFPRLARLKCLLLNNNRIVRIGENLHESLPNLQSIILTGNNIQELGDLEPLTKLPNLETLSLLTNPVSTKQHYREYVAFRFPNLRLLDFRKIRQKEREAANLLFKSKKGKEMHKEIVRKAKQALPAGALGESSPEKQAVQNASPADIQKIKEAIKRATNLHEVERLNRMLQSGQITGDILNGANGSEAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VTL9</accession>
    <name>A0A6E8VTL9_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PRKCA-binding protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Protein interacting with C kinase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Protein kinase C-alpha-binding protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="26 27">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="26">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="20">Probable adapter protein that bind to and organize the subcellular localization of a variety of membrane proteins containing some PDZ recognition sequence. Involved in the clustering of various receptors, possibly by acting at the receptor internalization level. Plays a role in synaptic plasticity by regulating the trafficking and internalization of AMPA receptors. May be regulated upon PRKCA activation. May regulate ASIC1/ASIC3 channel. Regulates actin polymerization by inhibiting the actin-nucleating activity of the Arp2/3 complex; the function is competitive with nucleation promoting factors and is linked to neuronal morphology regulation and AMPA receptor (AMPAR) endocytosis. Via interaction with the Arp2/3 complex involved in regulation of synaptic plasicity of excitatory synapses and required for spine shrinkage during long-term depression (LTD). Involved in regulation of astrocyte morphology, antagonistic to Arp2/3 complex activator WASL/N-WASP function.</text>
    </comment>
    <comment type="subunit">
        <text evidence="23">Monomer and homodimer. Interacts with CXADR. Interacts presynaptically with the glutamate receptors GRIA2, GRIA3, GRIK3, isoform 3 of GRIA4, isoform A of GRM4, GRM7 and GRM8; with NAPA and NAPB; and with BTG2. The interaction with NAPA and NAPB disrupts the interaction with GRIA2, conducting to the internalization of GRIA2. Interacts with PRKCA; with the amine transporters SLC6A2 and SLC6A3; with the channels ASIC1 and ASIC2; with the GTP-binding proteins ARF1 and ARF3; with the ephrin receptor tyrosine kinases EPHA7, EPHB1 and EPHB2; with ERBB2 and through its PDZ domain with the C-terminal tail of PRLHR. Interacts with UNC5A. Interacts (via AH domain) with NCS1/FREQ; in a calcium-dependent manner. Interacts with F-actin and associates with the ARP2/3 complex. Interacts (via PDZ domain) with ARF1 (activated); the interaction blocks Arp2/3 complex inhibition. Interacts with SORCS3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Membrane</location>
            <topology evidence="4">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="22">Postsynaptic density</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="21">Synapse</location>
            <location evidence="21">Synaptosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_040234761.2">
        <property type="nucleotide sequence ID" value="XM_040378827.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VTL9"/>
    <dbReference type="EnsemblMetazoa" id="ACON007832-RA">
        <property type="protein sequence ID" value="ACON007832-PA"/>
        <property type="gene ID" value="ACON007832"/>
    </dbReference>
    <dbReference type="GeneID" id="120956967"/>
    <dbReference type="CTD" id="9463"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_014528"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007832"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033602"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098842">
        <property type="term" value="C:postsynaptic early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008021">
        <property type="term" value="C:synaptic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032588">
        <property type="term" value="C:trans-Golgi network membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019904">
        <property type="term" value="F:protein domain specific binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005080">
        <property type="term" value="F:protein kinase C binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097062">
        <property type="term" value="P:dendritic spine maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002092">
        <property type="term" value="P:positive regulation of receptor internalization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043113">
        <property type="term" value="P:receptor clustering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034315">
        <property type="term" value="P:regulation of Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07659">
        <property type="entry name" value="BAR_PICK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06722">
        <property type="entry name" value="PDZ_PICK1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000023">
        <property type="entry name" value="Interacting with PRKCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000073">
        <property type="entry name" value="Interacting with PRKCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010504">
        <property type="entry name" value="AH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030798">
        <property type="entry name" value="Arfaptin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037959">
        <property type="entry name" value="PICK1_BAR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12141">
        <property type="entry name" value="ARFAPTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12141:SF1">
        <property type="entry name" value="PRKCA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06456">
        <property type="entry name" value="Arfaptin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01015">
        <property type="entry name" value="Arfaptin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50870">
        <property type="entry name" value="AH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="15" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="16" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="17" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0564">Palmitate</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0770">Synapse</keyword>
    <keyword evidence="8" id="KW-0771">Synaptosome</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PDZ" evidence="24">
        <location>
            <begin position="24"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="AH" evidence="25">
        <location>
            <begin position="146"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00031097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00032804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00033721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00034102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00034105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00093501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007832-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="481" mass="53736" checksum="F51A781BE408C542" modified="2020-08-12" version="1">MLQDCDYDYFYEEDKMGMTVSSGTVVIKKDTSNLIGISIGGGAPLCPCLYIVQVFDGTPAAREGTLQSGDELLGVNGVSVKGKTKVEVAKMIQSATEEVMIHYNKLHADPTQGETLDIVLKKMKHRLVERMSSSTADTLGLSRAILCNDSLVKRLQELERTETMYKGLVDHARRMLKAHFDVLQTYQAFGNIFASISVREPQPRASEAFRIFGELHRNMEKDGIKMIKTLKPILADMGTYLHKAIPDTKLTVKRYADAKFSYLSYCLKIKEMDDEEHGYAAIQEPLYRVETGNYEYRLILRCRQEARLKFAKLRSDVLEKIELLECKHARDLASQLRKFIEGLATLASETVERLESIPNLFPIEVDLKASAFQYKSAIKFQAEEYTDEEIPQAEEAIEQVETAKSAQAKTTGDLEQSNGTDVSGQLLGGFEEIDLNKGTSAGPSEHDLLNELGLAGIDLSVGSKPISHNLMDDLLVPELFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VUZ5</accession>
    <name>A0A6E8VUZ5_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="12">RBR-type E3 ubiquitin transferase</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RBR family.</text>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON007788-RA">
        <property type="protein sequence ID" value="ACON007788-PA"/>
        <property type="gene ID" value="ACON007788"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000096"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007788"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_029747"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071797">
        <property type="term" value="C:LUBAC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036435">
        <property type="term" value="F:K48-linked polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070530">
        <property type="term" value="F:K63-linked polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990450">
        <property type="term" value="F:linear polyubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097039">
        <property type="term" value="P:protein linear polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19815">
        <property type="entry name" value="Bbox1_HOIP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BRcat_RBR_HOIP"/>
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        </source>
    </evidence>
    <sequence length="3405" mass="377268" checksum="2CD83ABA70EEEE70" modified="2020-08-12" version="1">MASSPSNRFRSGRNMPSWVTESSDRVGPPPPPPPANNDPEYEVIDVANQQQYSNAPPPVPLKSPDIKRLTVMKCDLCGSVAPVVRCEQCDQNLFCVSCDDRYHRHPKRQTHVRKPIEPPAAVAPPTPSSTVKPPLPPKGDAGSSGPLPPPRRNKRPGSFHFPSPMFGRKQDQQTIPTTVPQGQPEQQQQKPPPPPPSPALSLREKMNSLKRFIHPSNRPLPDPPENKIHSSNSSLDTVSKRSPSIGPNRSVSSTMEKIQNNTAATLDRMTLLQQRYRQHQEAMKSDGDRSRRASLTSNTDLQSSATESSNSFRNKPTGAFQQQQQQHQQPSMQPSQQLQNRWLNPPVQPRIRSGSVASGINLLSVPGTPNGPGSGFGHNFAPPGNSNAPSVSPIQRSEFGDTMPSMPNGPRSLSTSVFNLHQPPPPQPNMWGFNPLHQAQSMAHFNPMQWNQMNAWMGRGSQNGSNMSLNLPPQHGYPPQDPAGYPPGWTNTWNGMYPYPPMGMMPMMPGTVEYIAIAVEPSLIPPSSQSPGVAMPPPRSRANSRSRAASPALSVKSRKSTMSMRNLNRNSFIDDLTDDEDSDDGFHRSRGRGGDRRRRERLNSTSSMDFDEPEPPAQSFVRASSVKHSRFNRDRRGGGSSARSLIDYPVSRKVTAPPARYDREELVRDRFDKLSVSSARKEPSDSFTNDSDLEQEGRRVTSNRSRSGNESLSSPRKINSDSMTNDSDADFERRRQAKLKAAQNNLAAARRITSDSHTNDSEPEQDRRRQQKSKPAPLPAPKKPIPSSDSDGREKQRRKSDTKKSDSGKAVAAAPKKIPSDSHTPDTDGSDQRRRFASKQQRQRKHSSTEFIANESDNERPEKVVNGLLEFRTISPTKSDSDEQRHLKTVEIKNIINDTRSEVEPDNGEVPEQSPPPPALVAPDREWECEFCTYVNEPGVKICAICCKTATISAVGGGATKQDGETRSPPPDVVQETPQVTEVKLQIVPPQKTANGKPPGETKKKDVDDEMDTEMDQIVEGEVVSKSCSDGSSEGLNRSLTPEKKQSVEIQPSFDADPVHVEKKVAKEKVSTGCGPSPPREIVGVGHSAAGGERTRHRASIGTSPPPQDMSTQTYDSFEQVRHEIATQSQRADIPANETAQEPNLTSSALYKRSYSLATPQLLEVERPASRNSISSDTQSLPPSPHELSPQPGLQNSSRYHANQALNQTTTSTTTQSSPSHRYGQQQQQDESLSFLDRAIHQIIQTAANQTLGAAAAATASGRTAAVPEENMYRTFNDLRRIDTMTQAKVVSPQPAGGGNVPPRQPMVTRQSKTMDDHQDPEDMPQGTEQLTEMQNGSTSKEEPSEMTLLLREAERYHFTAEELQAAMNHCGEKHPVQWLRSNWNKLIETVQTLATKYGHEKRENTIGTISTVEAREALRMHKGNIWRAITECIEQRQRKYREIASKGNFTREDIVTSLTAHHGNLELATIELSKTQLKPFLMRIWGPPSGADNDSGNLLLQQALQEDRTGISSEIQQFISAHVEQELLGTGPSEKVMQPSEPELPSAMEQADREDIEPEIQQLTEHENTKAPTPSLPEEDHLEEVCEAQSSTTTSNTEILKDIEALIMQMERKQESSNGTVLRNIQQLLSQLVDAEPNSRSPSATSIRSQASDQRIMSKSPIPVRNGKQPDHLPDPNRSIEDDVRDFVQENIQDILPNLVQQVRQELDAVKVNLQTKPKSLEDDIRETLMRAEYSENDYSNIHYFPFDKTPEPPVHFPERQQTSKVSVQPSVANETDEYANIQKFLERAIRNEKTTTVFDQMKRSNYLIDSSSDEARQTAESTDYYDLVSINEKLMHLFTKTPSKESQPVQQHEVASKTSHPEGSPSATKVQSHVQTAIEEATRTPQGPEEPIPQESPQMEKKGVKKRRGSRIPVNKNNYLYRRPVKSSSESDFEVLATNSKQNQSSIAAIDHVEEMRHVVENIERIEQTMDVEDAPEDEDDQEDFELVDEPIYINLPPKKESEAENVEQLEEFEDTTTMPQQASPVEESTTFVEPIKDHHTESVMQDLTQDVSTPQELPQQSVEATDAVNQPIVEEQKLQDSTPAISNTIQEPPIQPVESNTAAASIAHVVEEPKPIHSQDPAPQASNENQQQPLPAPSAEENIQTNELAITASNETNGHVQEEPQPTVIAETPVVASETIASSSAPNEAVKSEPPTNGSLPDNSNNISEADQQDDVISHSSKLANNLSELVLDTKRLIQQMKDEINSDIATFNEDDAAYEEDYYEDEYEDEWEGEEEEEEWDSNEEYDVDENGDFYEYEDDDDDGSVMFSEMTIIDSAKSRDAEQPPNEMIPVQEPPQMIMPMINMAIVRENERNSASEVSDLPLDSDFNDAMSVIEQSVGELRNMLHLTEEALGPLDLANPLHHASSVETLQNSPEQSEISEITLVPEYHNTTAEESSEPTLVADENVPMTMKDVQTLMNAKAVIGGPEYATVFKANSKLQEIETANDLAQQPPASIGTTLNTSSELPATNQGEAGLVNGTELRAAITEDASVPATSESYSVEQPSEATVNSQELQVSVESAIDDHLDHPASESLKMTNDITQTTQQSIQPESTQNQQGPGDLVDTSNNPIPVTDGDVQSLPQQPTTTNVTTEQQPPEPNANNLPNGNATSGHRTLSRASSEEPSSSFVEIQPSQVIIHTPQTETQPQVVPPEAPPRTTNIYQNVDLHPSASSISPTATTITTTSTTTTSTTTPMKSPPTRSKSKAPKLTVKVTNAASSQSEDGASPTTPPTPTGQKANAKATASKTKTESKKKSTSTTSSPTADSNGRRPSVTRKKSIGGVFGPVQTNTVKNMQKEFLNKAKESPKPSTSKVAPKPAKLVQPKAFTARSSTTPTASTSKASTPASEDASTSARSTPGLNDANEPQPGSSRDTTYMCEKLMKKKYRETCFSDEYQTTDDEDESHMTVTESERTIIKSLVKPYEPNNEPPEVQAKQLMEDGLVQTLAQAELAVQLIELRYAKDNAIWAATQCHTIDDAKDLLQKECELCLGVFPMNEIISMLKCTHTCCFECAKEYFTQEITNRSITNCNCPYCKEPDLNGPEVTEDDVLEYFSNLDILLKNIVDEEVHDLFQRKIRDRTLTKDPNFKWCVHCSSGFFARPKQRRLVCPDCGSITCASCRKAWETQHEGLTCEKFAEWKEANDPELQAEGVQRHLQTHGISCPNCKFRYSLARGGCMHFTCTQCKFEFCYGCNKPFMMGAKCSVSPYCAKLGLHAHHPRNCLFYLRDKEPRDLQNLLLMNNVPFDTEPSEQMKQELANGESTTMKCPIPLQKETPAGLMDMICSADVPEKHAGLCRTHYVEYLVGTVSKAQIDPLPILDLTDCVQELRRRGIPLPERGPWDTDEIYREMCQKLVKDKIPLDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VVQ6</accession>
    <name>A0A6E8VVQ6_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">CD109 antigen</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">TEP1-F</fullName>
        </alternativeName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="24">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Binds covalently through a thioester bond to the pathogen surface resulting in pathogen clearance.</text>
    </comment>
    <comment type="function">
        <text evidence="14">Modulates negatively TGFB1 signaling in keratinocytes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">Heterodimer of a TEP1-N chain and an TEP1-C chain non-covalently linked. Forms a complex composed of TEP1-N and TEP1-C heterodimer, LRIM1 and APL1C; the interaction stabilizes TEP1-N and TEP1-C heterodimer, prevents its binding to tissues while circulating in the hemolymph and protects the thioester bond from hydrolysis. Mature TEP1 and to a lesser extent full-length TEP1 interact with SPCLIP1; the interaction is induced by microbial infection.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Heterodimer; disulfide-linked. Interacts with TGFB1 and TGFBR1. Forms a heteromeric complex with TGFBR1, TGFBR2 and TGFBR3 in a ligand-independent manner.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.</text>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON008364-RA">
        <property type="protein sequence ID" value="ACON008364-PA"/>
        <property type="gene ID" value="ACON008364"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000438"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008364"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038887"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004866">
        <property type="term" value="F:endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02897">
        <property type="entry name" value="A2M_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.20:FF:000001">
        <property type="entry name" value="CD109 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1930:FF:000001">
        <property type="entry name" value="CD109 isoform 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000155">
        <property type="entry name" value="complement C3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.130.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1930">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.690">
        <property type="entry name" value="Alpha-macroglobulin, receptor-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009048">
        <property type="entry name" value="A-macroglobulin_rcpt-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036595">
        <property type="entry name" value="A-macroglobulin_rcpt-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050473">
        <property type="entry name" value="A2M/Complement_sys"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011625">
        <property type="entry name" value="A2M_N_BRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041813">
        <property type="entry name" value="A2M_TED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047565">
        <property type="entry name" value="Alpha-macroglob_thiol-ester_cl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011626">
        <property type="entry name" value="Alpha-macroglobulin_TED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001599">
        <property type="entry name" value="Macroglobln_a2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019742">
        <property type="entry name" value="MacrogloblnA2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002890">
        <property type="entry name" value="MG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041555">
        <property type="entry name" value="MG3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040839">
        <property type="entry name" value="MG4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11412:SF136">
        <property type="entry name" value="CD109 ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11412">
        <property type="entry name" value="MACROGLOBULIN / COMPLEMENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00207">
        <property type="entry name" value="A2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07703">
        <property type="entry name" value="A2M_BRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07677">
        <property type="entry name" value="A2M_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01835">
        <property type="entry name" value="MG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17791">
        <property type="entry name" value="MG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17789">
        <property type="entry name" value="MG4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07678">
        <property type="entry name" value="TED_complement"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01360">
        <property type="entry name" value="A2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01359">
        <property type="entry name" value="A2M_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01361">
        <property type="entry name" value="A2M_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01419">
        <property type="entry name" value="Thiol-ester_cl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49410">
        <property type="entry name" value="Alpha-macroglobulin receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00477">
        <property type="entry name" value="ALPHA_2_MACROGLOBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8" id="KW-0391">Immunity</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0882">Thioester bond</keyword>
    <feature type="domain" description="Alpha-2-macroglobulin bait region" evidence="21">
        <location>
            <begin position="457"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-2-macroglobulin" evidence="22">
        <location>
            <begin position="686"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-macroglobulin receptor-binding" evidence="23">
        <location>
            <begin position="1305"/>
            <end position="1394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1416"/>
            <end position="1438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="1424"/>
            <end position="1438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00056820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00057615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00063008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00063781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00069665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00078071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="SMART" id="SM01359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="SMART" id="SM01360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="SMART" id="SM01361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008364-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1462" mass="162077" checksum="955189D565DB1A52" modified="2020-08-12" version="1">MRRSTMFASKKDPAIRILSTSSSSLLAVCLVLSVVLVPAVQCEGHYSIVGAKLLRPNSEYHVAVTNQDVSEPIRFSLAITDASSVIAKQEITLNTGETRLVPFAIGDIPESSYKLVAEGLSGLTFKNETDLEYQQKSFSVFVQTDKSIYKPGDTVRFRVLVLDPNTKPLQKADNISVHINDAKANRIKQWKEGKLVKGVFESELTLSTAPVLGAWTINVEVLGSKHNKVFEVDEYVLPKFEVTVESPGITTFKDGKVKAIIRSKYTYGKPVKGEATVSVSPEFQFNYVQPFAKDVITRKVIPIDGKGSVEFDLREDIHLEGDYSRNIVIEAVVEEELTGRKQNASAKVMIYDRRYKMELVKSDDNFKPGLPYTAWLKVSYQDGAPVQDQTNPVEVKQSSFESTTSVQNYTLDQNGMAKLEINTEVNSSYINVVGVYLGQEFYLHGISKAESDVDSYIRAQVLTEMPLVGKDVLVEVTSTSPMKYFTYQLLGRGDVLLSNTIAVPESKTQSFKFPATFAMVPRAKLVVYYIASNGDMVSDSKVITFDSELQNFMKVSLSKEQSKPGQDVEISISTNPDSYVGLLGVDQSVLLLKSGNDITKQQVFSELEKYEERSYGFYRRKKRFAWNPHAEHRDFSTVGAFVMSNANDPPQLAFPEAIAFASPSLGASAGIPSADGPTVRKDFPESWIWYSAAEEGFSGEKTLQKKVPDTITSWIITGFSVNPIYGLGLTQQPRKLNVFLPFFVSTNLPYSVKRGEVVAIPIVVFNYMEDDQTAEVVLHNDEQEFEFADVENEVVESNKVELFRQKRLDIASNTGKSVSFMVKPKKLGHITIKVTAKTKIAGDAVERQLLVEPEGLPQFINKAAFIDLRAAPELTKTFEVEIPKNAVPDSTRIEVAVIGDVMGSTIQNLDSLIRMPYGCGEQNMLNFVPNIVVLDYLKATNKLTANIEAKAKKFMEAGYQRELGYKHRDGSFSAFGENDKSGSTWLTAFVARSFKQAANHITIDEGVIDKSLEWLSDHQAPNGSFPEVGVVSHKDMQGGSGSGVALTAYTLIAFLENINLVDKYKNTINKAIDYVYRNTESLDDTYALALAAYALQLADHSSKGLILSKLDTKATTDSDSKWWHKPIPETEQKNPWYSRPNSVNVEMSAYGMLAFLEAGLDTDALPIMKWLIGQRNDKGGFQSTQDTVVGLQALAKLAAKITSPNNDVTLTAKINENQEKRMTVNAENGMILQKFELPSAARNIEIQATGSGFAVVQLSYKYNMNVTGEWPRFVLDPQVNANTNPDYLHLSVCASFVPSAGQNVSNMAVMEVGFPSGFTADSDTLPSLENMPFIKKVETKDGDTTVVLYFDSLDQRELCPTISAFRTHKVAKQKPAPVVIYDYYDNSRIARQFYDGPKASLCDICENEDCGEACSIRSQKQRSSDSPSRQPTVEGTMQSGSQTVSVSFFTFLLATLLVRMFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VVQ7</accession>
    <name>A0A6E8VVQ7_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Secreted protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VVQ7"/>
    <dbReference type="EnsemblMetazoa" id="ACON008013-RA">
        <property type="protein sequence ID" value="ACON008013-PA"/>
        <property type="gene ID" value="ACON008013"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007974"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008013"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037907"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026112335" evidence="2">
        <location>
            <begin position="19"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="141"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="398"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="462"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="613"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="414"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="637"/>
            <end position="646"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008013-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="701" mass="74329" checksum="21D4ADC3A99BB8E0" modified="2020-08-12" version="1">MWKVCVVLGALLLGYSNAGIASWSEDISVHNKHYSQHESSVNAARKAAAERLQQHQAAALVAGSGSSTGPCDTLCYNSYAADAGSDAETIGQGIVGFTSTGAAGAVGSSQKYSASSSRVSESSVQNVAVPVVYPAVASTAGTRSSYSSSSSSRQQKVHSSAGVAQPVSGALYSADHGLTRSSIVQPVVYPVAAPQTHAAQSSYERSSSERSVVSRPVVNSVVYTPLPVAGATNSYNTRSSFAAASNEQQVVQPVTYPAANNDYSRRYVSQQDHTQYRQYPSTSNTYVLYSKPIASSVQYYAPSRSRTEEAASSTSYDASGRVVNLNIAQPVHSERVNEQRSQQRAESVQTIQRQSDLYPVRAGYDHQEKEAVQHHQEVDYEDTVDTLVTDRPVRLLTPTVPAASSASSQRRTEQQSSYVRTGSYVPNTGSSTRYNTAQSMEGQRRQTYHSAPAYVAPVPVTGSVSSSKYESAAESQQRHTQNSLNVPIVVQPAPVSASSTASRYASSAHEQRTGASGPVYYAPAAGASTYGSQYSNTAASSSHNSRYKAGSAGGFVHYPIANDDFGRRFGAVGGAGGFGAETDLQDIMSESEALARLQAQNVHNGAVTGSGTIDAETRFGGESDGLGTMPGGYQRTKSWSSSSKWASEQRYGDDGKPKTFNMLSTAESEKHNINGKTTGYKAATTTLDDDGKVSTYSLHTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VVQ8</accession>
    <name>A0A6E8VVQ8_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Forkhead box protein L2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_040237096.1">
        <property type="nucleotide sequence ID" value="XM_040381162.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VVQ8"/>
    <dbReference type="EnsemblMetazoa" id="ACON008606-RA">
        <property type="protein sequence ID" value="ACON008606-PA"/>
        <property type="gene ID" value="ACON008606"/>
    </dbReference>
    <dbReference type="GeneID" id="120958395"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011049"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008606"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_029538"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd20028">
        <property type="entry name" value="FH_FOXL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000016">
        <property type="entry name" value="Forkhead box protein I1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047515">
        <property type="entry name" value="FH_FOXL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001766">
        <property type="entry name" value="Fork_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050211">
        <property type="entry name" value="FOX_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018122">
        <property type="entry name" value="TF_fork_head_CS_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030456">
        <property type="entry name" value="TF_fork_head_CS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11829">
        <property type="entry name" value="FORKHEAD BOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11829:SF411">
        <property type="entry name" value="FORKHEAD BOX PROTEIN L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00250">
        <property type="entry name" value="Forkhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00053">
        <property type="entry name" value="FORKHEAD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00339">
        <property type="entry name" value="FH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00657">
        <property type="entry name" value="FORK_HEAD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00658">
        <property type="entry name" value="FORK_HEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50039">
        <property type="entry name" value="FORK_HEAD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5" id="KW-0221">Differentiation</keyword>
    <keyword evidence="8 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="10 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Fork-head" evidence="14">
        <location>
            <begin position="230"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Fork-head" evidence="12">
        <location>
            <begin position="230"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="83"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="450"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="83"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="94"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="157"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="455"/>
            <end position="474"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008606-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="474" mass="53056" checksum="37DCEC302C15962C" modified="2020-08-12" version="1">MLATTANSDMAPPDGPMQYDSKWSPMNIISMPTKIKQEGFNVLSHLDVYHPLAVSLPAYDQVKSSSPSSLQQLPTVIERLHEETGSSPDHQHQHLVQQQHQQQHQQQQHQQQQQQQQQLHHNIGSLALSSSKPSSQGPDELPSNSLAPDELHGGQIPTTLPQPNSTNNNNNNHTPNQNQTTSNQSSTNSTTSNGGASTVSNSSTTNSSSLSTYDCSSTTSPTGSRNSNVKPPYSYVALIAMAIQSSQMKRATLSEIYGYITSRFPYYEKNKKGWQNSIRHNLSLNECFVKIPREGGGERKGNYWTLDPQYEDMFENGNYKRRRRMKRPYRTGHYSKMFGDSYVTNPGNFGHRPVFAQNPYQTYPRYDAGAPWMPTTQLSSYTSCSSRTNYTYSAPLQQSVQPVTINTYGSLSNNLDTFAVSGSTTPPCARRFEPHYPYWDTTLPLPVVKDEPTNQMSVSSSQQNSYQKSYLSQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VXA7</accession>
    <name>A0A6E8VXA7_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CAP-Gly domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
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    <dbReference type="EnsemblMetazoa" id="ACON009210-RD">
        <property type="protein sequence ID" value="ACON009210-PD"/>
        <property type="gene ID" value="ACON009210"/>
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    <dbReference type="VEuPathDB" id="VectorBase:ACON009210"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041357"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.190">
        <property type="entry name" value="CAP Gly-rich-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR000938">
        <property type="entry name" value="CAP-Gly_domain"/>
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    <dbReference type="PANTHER" id="PTHR18916">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RESTIN HOMOLOG"/>
        <property type="match status" value="1"/>
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    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="39"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="CAP-Gly" evidence="2">
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            <begin position="168"/>
            <end position="210"/>
        </location>
    </feature>
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        <location>
            <begin position="89"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009210-PD"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="264" mass="27591" checksum="6F2FCF816E68E5A8" modified="2020-08-12" version="1">GRRRSSDQGAVLTADTDSFIIGQRVWVGGIRPGQIAYIGETHFAPGEWAGVVLDEPNGKNDGSVAGKRYFQCEAKKGVFSRLTRLTREPLANAGGGGGDSTASTPLDASFRSLTSPARSGTVSPTHSVQSYASKSPAMAGKAASLNVGDRVIVSSGFGSRPGMLKFIGETQFASGTWCGVQLDEASGKNDGTVDGVRYFECPAKYGIFVPIAKVTLSPSARKTSARLSRANSKESLNSLATMSSIATTATSRLRMSAQVSLAAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VY55</accession>
    <name>A0A6E8VY55_ANOCL</name>
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            <fullName evidence="2">CAP-Gly domain-containing protein</fullName>
        </recommendedName>
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        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
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            <taxon>Metazoa</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
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            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
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        <property type="gene ID" value="ACON009210"/>
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    <dbReference type="VEuPathDB" id="VectorBase:ACON009210"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041357"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.190">
        <property type="entry name" value="CAP Gly-rich-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036859">
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    </dbReference>
    <dbReference type="InterPro" id="IPR000938">
        <property type="entry name" value="CAP-Gly_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18916">
        <property type="entry name" value="DYNACTIN 1-RELATED MICROTUBULE-BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18916:SF93">
        <property type="entry name" value="RESTIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01302">
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01052">
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74924">
        <property type="entry name" value="Cap-Gly domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00845">
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    </dbReference>
    <dbReference type="PROSITE" id="PS50245">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="175"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="CAP-Gly" evidence="2">
        <location>
            <begin position="304"/>
            <end position="346"/>
        </location>
    </feature>
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            <begin position="1"/>
            <end position="141"/>
        </location>
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            <end position="267"/>
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            <begin position="32"/>
            <end position="44"/>
        </location>
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            <end position="97"/>
        </location>
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            <end position="119"/>
        </location>
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        <location>
            <begin position="236"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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        </source>
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        <source>
            <dbReference type="PROSITE" id="PS50245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009210-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="499" mass="52678" checksum="3E0344650616A8EC" modified="2020-08-12" version="1">METEANAPEPAPTAANGEETSTPVQPPAPANGGAGVVVVGGGSGIQPPKVRALPKPSGIRPPTANLHGGSTTSLASTSSQLTAAPSTTSSSAGAAATRIGRLCTGHTPPKAGPPPPEPKLPTTFDSKMRRPSDSDYNKTHLPDQGAVLTADTDSFIIGQRVWVGGIRPGQIAYIGETHFAPGEWAGVVLDEPNGKNDGSVAGKRYFQCEAKKGVFSRLTRLTREPLANAGGGGGDSTASTPLDASFRSLTSPARSGTVSPTHSVQSYASKSPAMAGKAASLNVGDRVIVSSGFGSRPGMLKFIGETQFASGTWCGVQLDEASGKNDGTVDGVRYFECPAKYGIFVPIAKVTLSPSARKTSARLSRANSKESLNSLATMSSIATTATSRLRMSAQRKSTVSAVPSTPKSSFSLQDVLREKQNHIEQLMQEREIDREETANQSIMYQKNIQQRDTTDGGEVGRWAELVRRRALRIADLRREFQGWHGNHERHCILHDVLLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VZC7</accession>
    <name>A0A6E8VZC7_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Mitochondrial carrier protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
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            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
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    <dbReference type="AlphaFoldDB" id="A0A6E8VZC7"/>
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        <property type="protein sequence ID" value="ACON009333-PA"/>
        <property type="gene ID" value="ACON009333"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_013994"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON009333"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035392"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015218">
        <property type="term" value="F:pyrimidine nucleotide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990519">
        <property type="term" value="P:pyrimidine nucleotide import into mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049562">
        <property type="entry name" value="SLC25A33/36-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45829">
        <property type="entry name" value="MITOCHONDRIAL CARRIER PROTEIN RIM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45829:SF4">
        <property type="entry name" value="MITOCHONDRIAL CARRIER PROTEIN RIM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
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    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
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        <location>
            <begin position="6"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="180"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009333-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="338" mass="36534" checksum="C4AFBE640FDDF707" modified="2020-08-12" version="1">MSQRDRESFIHLFAGGIAGTAGAVVTCPLEVVKTRLQSSSSSFIHAAASTRHAALAADGGKLTDHHHVRHVSATVNSASSAADRHHSAARHQQSRVCASTILTRRRPSILAIPQCGLSTSVQSISIWQCLKHIVQTEGSRALFKGLGPNIVGVAPSRAIYFCAYSKTKNSLNTVGIIPANSPLVHILSASCAGFVSSTATNPIWFIKTRMQLDSNARMTVGECVRRIYESQGVRGFYKGITASYVGISETVIHFVIYEALKKKLSVGIVRSRSSSGVVLVVHHRVVSCVCVCCSCHCLRGFHDDGDVCMLRCARAMCKIENGCDRNSVPEILTESERW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VZE4</accession>
    <name>A0A6E8VZE4_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glyoxylate reductase/hydroxypyruvate reductase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040236312.1">
        <property type="nucleotide sequence ID" value="XM_040380378.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040236313.1">
        <property type="nucleotide sequence ID" value="XM_040380379.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VZE4"/>
    <dbReference type="EnsemblMetazoa" id="ACON009610-RA">
        <property type="protein sequence ID" value="ACON009610-PA"/>
        <property type="gene ID" value="ACON009610"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON009610-RB">
        <property type="protein sequence ID" value="ACON009610-PB"/>
        <property type="gene ID" value="ACON009610"/>
    </dbReference>
    <dbReference type="GeneID" id="120957918"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005151"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON009610"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038506"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030267">
        <property type="term" value="F:glyoxylate reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008465">
        <property type="term" value="F:hydroxypyruvate reductase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05301">
        <property type="entry name" value="GDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000026">
        <property type="entry name" value="Glyoxylate/hydroxypyruvate reductase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050223">
        <property type="entry name" value="D-isomer_2-hydroxyacid_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029753">
        <property type="entry name" value="D-isomer_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996">
        <property type="entry name" value="2-HYDROXYACID DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996:SF119">
        <property type="entry name" value="FI03731P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00671">
        <property type="entry name" value="D_2_HYDROXYACID_DH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase catalytic" evidence="4">
        <location>
            <begin position="18"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding" evidence="5">
        <location>
            <begin position="115"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00073306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009610-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="325" mass="35016" checksum="ED46AEECC7A2998A" modified="2023-06-28" version="2">MVNSGQRPVVLVTNKETPEKGINKLKLKADVIFPKSHPATREEVLELAGQVDGIMWVGHMPLNGEVLDRGGSRLKAISTMSAGMDYVDVEEFRKRKFPLGYTPIVLNDAVADSAIGLMIAAGRRYHEGRLAIDQSQWTGGPQWLLGQDIKGSTVGIIGLGGIGQTIAKRLKGFDIGQLLYTGRRPKPEAEALGAKLVPQDELLQESDYVFITVPLTNETRHMINESTLAKMKPTAVLVNVARGDIVDQRALVAALKNGTIFAAGLDVVSPEPLPADDELLRLPNAVVIPHLGSATVQTRNNMAEIAALNVLAGIAGTPMFSPYPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VZF0</accession>
    <name>A0A6E8VZF0_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Piezo-type mechanosensitive ion channel component</fullName>
        </submittedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PIEZO (TC 1.A.75) family.</text>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON009276-RC">
        <property type="protein sequence ID" value="ACON009276-PC"/>
        <property type="gene ID" value="ACON009276"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_014416"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON009276"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033363"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008381">
        <property type="term" value="F:mechanosensitive monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027272">
        <property type="entry name" value="Piezo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031334">
        <property type="entry name" value="Piezo_cap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056770">
        <property type="entry name" value="Piezo_THU9_anchor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056769">
        <property type="entry name" value="Piezo_TM1-24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031805">
        <property type="entry name" value="Piezo_TM25-28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056768">
        <property type="entry name" value="THU_Piezo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47049">
        <property type="entry name" value="PIEZO-TYPE MECHANOSENSITIVE ION CHANNEL HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47049:SF2">
        <property type="entry name" value="PIEZO-TYPE MECHANOSENSITIVE ION CHANNEL HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12166">
        <property type="entry name" value="Piezo_cap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24874">
        <property type="entry name" value="Piezo_THU9_anchor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24871">
        <property type="entry name" value="Piezo_TM1-24"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15917">
        <property type="entry name" value="Piezo_TM25-28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23188">
        <property type="entry name" value="THU_Piezo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="30"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="58"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="115"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="193"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="220"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="244"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="316"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="506"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="534"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="662"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="687"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="717"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="936"/>
            <end position="952"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="973"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1038"/>
            <end position="1057"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1095"/>
            <end position="1113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1147"/>
            <end position="1175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1270"/>
            <end position="1287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1293"/>
            <end position="1314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1326"/>
            <end position="1345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1742"/>
            <end position="1763"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1900"/>
            <end position="1920"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="2096"/>
            <end position="2118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="2138"/>
            <end position="2158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="2167"/>
            <end position="2188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="2200"/>
            <end position="2217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="2309"/>
            <end position="2332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="2580"/>
            <end position="2599"/>
        </location>
    </feature>
    <feature type="domain" description="Piezo TM1-24" evidence="16">
        <location>
            <begin position="26"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="Piezo TM1-24" evidence="16">
        <location>
            <begin position="440"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="domain" description="Piezo TM25-28" evidence="14">
        <location>
            <begin position="1249"/>
            <end position="1526"/>
        </location>
    </feature>
    <feature type="domain" description="Piezo transmembrane helical unit" evidence="15">
        <location>
            <begin position="1855"/>
            <end position="1975"/>
        </location>
    </feature>
    <feature type="domain" description="Piezo THU9 and anchor" evidence="17">
        <location>
            <begin position="2094"/>
            <end position="2331"/>
        </location>
    </feature>
    <feature type="domain" description="Piezo non-specific cation channel cap" evidence="13">
        <location>
            <begin position="2370"/>
            <end position="2668"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="150"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="805"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="848"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1571"/>
            <end position="1644"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1657"/>
            <end position="1685"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1692"/>
            <end position="1711"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="2666"/>
            <end position="2692"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="1444"/>
            <end position="1471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="158"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="1580"/>
            <end position="1590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1628"/>
            <end position="1644"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="1657"/>
            <end position="1674"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF12166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF15917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF23188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF24871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF24874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009276-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="2692" mass="307878" checksum="A7DFE495C3B4190A" modified="2020-08-12" version="1">MANYFLCLVVQRLVLPLILATCVLMRPVGLAIPYLICLLVIPYVPVANKKTIQGPTGLFMRLLILFVVLDLAAVIVFQLILISFPGSSVESCSLGEIILRHIGLVSFNGLGVWMVVYWLAPEAILLVGAVTVHIVLRKLTGVIVPRRRRRRRGADGNTAEDDTEQGADAEGEGPAAEAEESELQTSLISPERLPFFTTLGVLLSMCMLCVVGIIQPSAISGVYFVVFLGAASWWGCYKKLDRAFGIVLKIVLVVLVIHIVLLLAYQNPWPQEVLYFNDTAARVLALKPILNSSCNLANGTDIRDVHFNSDLDTDVYLNPIFLLLCYYTLTMTSSLLLRTRSDTTKDRKRLDLSSQLENGIRLQNQISFRMQRKNILRKTLTSDRWRGAARKVKSQSNGPSARGYYTYGRSTTYGRGNSTINTDIPDERTPLMRRGTHRIRDASANDGKDCAQTDQIALESLEEEEPSSFFEQALLAIRNVASYIYKNSYIFTNVIMMVWSIMYHSWLTFVLLLWANLIWILPNHRKNMLNSSPFLVIYAEVLLIAQFLYGMNLTEAELPTVVEGSGINLEQLGFVRRTEYPIVPLLVKSLFTTMFWISLRQLIQEKQTERRESMIANMAAPLQLPVGAATTAATGPPSATERLPEKKSSAIVDRMAKFFRSFMIKFWIWVVALTLFLSAIIGNRMTVFRIVYMVLFLCFLLSFQVWKEIDVWRRVMFTFWITVILYSMAILILVYLHQFEQFPRYWAAIGISKELQEDIGLEIFKTGQLFLHLVNPTLVVIITVIQLHYCHDRFLIISEIPSIQTETEQSDEEDKQEPVAPATDGPAYGTFTNADNGAIMDSTEAVPSATDTTVGQKADAQPAREKLPLEEDDDDEEQIEKIEDFRFRKLSRQEITGFARKAWKMALDLLELTWLFFEIHLLKIVLIVAFSLSVNSVNFLHLLYVVLSVFTVKAHTSGKDSLTYIYQIRITRIMSLFSAIMLIITMIYQVKYIKEGDFQSECPNIDANTTLMDMNNAKWIGMRKTGSGETLSDLLRPYLVYILLVTVHQVIILRQTIHRMRLGRSPKTPSLMFPNIVRADADKDIPRMIKYLFNYGYYKFGIEISLVALIVVVGNRMDVLSIFYAIWLAVMFHMTRDGLQRLWRWLTWFIVAVIPIQYVLFVGLPPFACVNYPWFVPQLDHFRIWAMLPENSYEFRSFANKMVSDFVLLMFLCRQTVVFRLEREPPRRFGGGSNQSVLGDFQKLDEGNFQNPTPDFITKVRNWLDMLKRTLFLVSFWFTLAVVFLSGSSRVNLFSIGYLIGAFFFLWQGTDFYLHSIEYILKRWNILICYNVFVIGAKAFLQLFGCLFLEQLVNNNCWVMQLFGMNCLSSALPPLPPGTVDPGEQCNIPPASDAGLFWDGMCFAFLLLQRRLFSSHYFCHIINESKASKYLASRGAELIEELRIKESAQEAERERQILEKIKAKMDRIKATQQKILEAVQDPATHAIDTVDHRSSLKRKISEHRPTAIRSGDYYMFEDVEQEFELDLMEPHEYDDELDERSQKDQRLRRRLTLGRLAAQLRKQRQQEVYELAYGKRDANEPDDDDDDDDTNAAVDQPLTLASSDLPAEEDPAQPPALRRRRLSSPVVLRRDHPTHTDHRGTDDRTAALERSALSEPLPPIEAQYPPPPPQPLPTLPQTAHSGTRLRKPTALDAPDAQTAGPSKELHAQHSITDEEVRLLSDEEDEVEGRKEADVAEEKKSKFSLSGLALIPAFLAGVVGSVTLRLHRASRNYRYVMRVLTREKQVLKETPGFGVGVRDKDGVWTHYISRSGSKTDGSSTEAVKRTLDEVSSEEDFSSRKHSVLFELFQAAWYAIMSHTDFICYFVVFLNQVNSASLLSLPLPLLVTLWGTLTFPRPAQTFWTMLIAYTQMVVLVKCIGQFEMFWWNQTPIPSNQPFHVARLFGIERKDGYATYDLILLLMLYCHRFILKSLGLWKSDPTEEPALSEGLYQVDPNDERAKALMALAEENEKKRETLEIRPPEELTGLSMVKINEKAGTIVEEPIGEESNVLCVSIHPEPTKKYFPQIAKEAFLRYSAALKAFFVQLLDRQSRKTTDVYGYMFLCDFINFFVILFGFSSFGTQDGDGGVLSYFEENRVPVTFLLMLIIQFFLIVVDRALYLRKNIQGKIIFQFILIIGLHVWMFFVLPATTERNFNATTPPVLYYMIKCFYLLFSAYQIRCGYPARILGNFLTKGFTMANFSGFKLFMTVPFLFELRTLMDWIWTDTSMTLFDWLKMEDIFSNVYQLKCMRQLEEDLPAPRGQKKGAMVKYLMGGGMMLGIIFLIWFPLALFAFSNAVGQPNIPHDVSVELRIGTYEPVYAMSAQNNSIYGLTPNNWSNFTAPFMERSAQTFLSNYEPADVAAVQLGISSASIWNISPPDRNRLLYDLINNVTLTCRFRYTISRMTNSKENPGVISEERTYQLYDGPARQALINSLSRTKPDEMAVLMNIMPKFLRVQNSGSIRPVHQLVKTADGDEDEENYRNMQLRQLYMDETSNTSWWQAMEDCTDTLYEKYFSRLPYADCTNFLVIYMFNDKIFPSTISSIAAGGIIGIYSTMILVFSRMLRTSIFSGASSKIMFEDLPYVDRVLQLCLDIYLVRESSEFTLEEDLFAKLLFLYRSPETMIKWTRPKNEGGGDDETDTMTDQPETHPKQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W0N1</accession>
    <name>A0A6E8W0N1_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Splicing factor, arginine/serine-rich 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the splicing factor SR family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8W0N1"/>
    <dbReference type="EnsemblMetazoa" id="ACON010496-RA">
        <property type="protein sequence ID" value="ACON010496-PA"/>
        <property type="gene ID" value="ACON010496"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012562"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON010496"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038646"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12601">
        <property type="entry name" value="RRM2_SRSF1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000053">
        <property type="entry name" value="Serine/arginine-rich splicing factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050374">
        <property type="entry name" value="RRT5_SRSF_SR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23003">
        <property type="entry name" value="RNA RECOGNITION MOTIF RRM DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23003:SF62">
        <property type="entry name" value="SERINE_ARGININE (SR)-TYPE SHUTTLING MRNA BINDING PROTEIN NPL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 8" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="10">
        <location>
            <begin position="61"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="22"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="131"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="32"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="42"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="131"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="159"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010496-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="178" mass="20325" checksum="94CD0C1E5C0371C7" modified="2020-08-12" version="1">DADDAVKARDGYDYDGYRLRVEFPRGGGPGSYRGSRQGNSDRNSRGGDRNNRGPPARRSQFRVMVTGLPSSGSWQDLKDHMREAGDVCFADVYKDGTGVVEFLRHEDMKYAIKKLDDSRFRSHEGEVAYIHVREDSGNDDSGKRDYRDRSYSPRRRRGSPTYSPVQRSVSRSRSRSYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W0N4</accession>
    <name>A0A6E8W0N4_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ornithine decarboxylase antizyme</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">Ngousso</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Interacts with ODC1 and thereby sterically blocks ODC homodimerization.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ODC antizyme family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8W0N4"/>
    <dbReference type="EnsemblMetazoa" id="ACON010131-RA">
        <property type="protein sequence ID" value="ACON010131-PA"/>
        <property type="gene ID" value="ACON010131"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012101"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON010131"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_029919"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008073">
        <property type="term" value="F:ornithine decarboxylase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045732">
        <property type="term" value="P:positive regulation of protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0075523">
        <property type="term" value="P:viral translational frameshifting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.60:FF:000003">
        <property type="entry name" value="Ornithine decarboxylase antizyme 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002993">
        <property type="entry name" value="ODC_AZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038581">
        <property type="entry name" value="ODC_AZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10279">
        <property type="entry name" value="ORNITHINE DECARBOXYLASE ANTIZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10279:SF10">
        <property type="entry name" value="ORNITHINE DECARBOXYLASE ANTIZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02100">
        <property type="entry name" value="ODC_AZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01337">
        <property type="entry name" value="ODC_AZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0688">Ribosomal frameshifting</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="7"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010131-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="253" mass="28412" checksum="D157423904FB3887" modified="2020-08-12" version="1">MIYPRGYSSSRIKSKENQQPSFSSKMPSLSFNMREPSLSSSNRTSKRTISSSSSSSAGSDSYCVSLADRCGGPDVPTIRTDHDRASPLKEYNRKDSFDSTTTTASSEYYDLDFPESTVDYLNHQEAAVIQEVLSQSAPTPITLKLFVTPQKCSSWETVFNPIENILYVSLPSAMSHEASKHSFISLLEFAEEKLECDAVVLCIRKDRLDRPNLVRTFSFVGFQPLSPKSPLAPPHIDEQQKNEYLFMVYSIEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W0Q7</accession>
    <name>A0A6E8W0Q7_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Kinesin-like protein unc-104</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Required for presynaptic maturation, has a role in axonal transport of dense-core vesicles carrying synaptic vesicle precursors, components required for the morphological transformation of axonal growth cones to mature boutons.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON010519-RG">
        <property type="protein sequence ID" value="ACON010519-PG"/>
        <property type="gene ID" value="ACON010519"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001094"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON010519"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037311"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904115">
        <property type="term" value="C:axon cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048490">
        <property type="term" value="P:anterograde synaptic vesicle transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021700">
        <property type="term" value="P:developmental maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051222">
        <property type="term" value="P:positive regulation of protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040012">
        <property type="term" value="P:regulation of locomotion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051960">
        <property type="term" value="P:regulation of nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010975">
        <property type="term" value="P:regulation of neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008582">
        <property type="term" value="P:regulation of synaptic assembly at neuromuscular junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd22705">
        <property type="entry name" value="FHA_KIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01365">
        <property type="entry name" value="KISc_KIF1A_KIF1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01233">
        <property type="entry name" value="PH_KIFIA_KIFIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.20:FF:000001">
        <property type="entry name" value="Kinesin family member 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000004">
        <property type="entry name" value="Kinesin-like protein isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000204">
        <property type="entry name" value="kinesin-like protein unc-104 isoform X6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2520">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022164">
        <property type="entry name" value="Kinesin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022140">
        <property type="entry name" value="Kinesin-like_KIF1-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032405">
        <property type="entry name" value="Kinesin_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049780">
        <property type="entry name" value="PH_KIFIA_KIFIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47117:SF10">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIF1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47117">
        <property type="entry name" value="STAR-RELATED LIPID TRANSFER PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12473">
        <property type="entry name" value="DUF3694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12423">
        <property type="entry name" value="KIF1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16183">
        <property type="entry name" value="Kinesin_assoc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="9 12" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="16">
        <location>
            <begin position="3"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="15">
        <location>
            <begin position="1677"/>
            <end position="1775"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="391"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="887"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1087"/>
            <end position="1119"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="359"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="617"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="398"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="887"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1103"/>
            <end position="1118"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="97"/>
            <end position="104"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00056070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010519-PG"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1779" mass="199822" checksum="76B68284B2454D94" modified="2020-08-12" version="1">MSSVKVAVRVRPFNSREIARESKCIIEMAGNTTCITNPKVPPGSSESVKRFNYDYSYWSHDPRDLEFSTQAMVYSDIGEEMLQHSFDGYNVCIFAYGQTGAGKSYTMMGKQEDGQEGVIPMICKDLFRRIQETESDDLKYSVEVSYMEIYCERVRDLLNPKNKGNLKVREHPLLGPYVEDLSKLAVTSYQDIHDLIDEGNKARTVAATNMNETSSRSHAVFTIFFTQKRQDRMTSLETEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEIASKNKKSKKADFIPYRDSVLTWLLRENLGGNSKTAMIAAISPADINYDETLSTLRYADRAKQIVCKAVVNEDANAKLIRELKEEIQKLRELLKAEGIEVQEGEDGEKKIHSPNRNRKRTGSSTEMAVDQLQASEKLIAELNETWEEKLKRTEQIRVQREAVFAEMGVAVKEDGITVGVFSPKKSPHLVNLNEDPTLSECLLYYIKDGLTRLGTSEANVPQDIQLSGSHILKEHCVFENKDGVVTLVPHKDALVYVNGRKVIEPEVLQTGSRVILGRNHVFRFTHPEQAREKREKNKEVDVCETPGNSGEIADWNFAQCELLEKQGIDLKAEMEKRLLALEEQYKREKRAADQEFEEQRKTYEARIDALQKQVEEQSMTMSMYSSYSPEDFHQEEDIFVNPLFESCWTAREAGLAAWAFRKWRYHQFTSLRDDLWGNAIFLKEANAISVELKKKVQFQFTLLTDTLYSPLPPELTPAPAVGALTNGGQEDEFGQNPIPRTTVAVEVTDTKNGATHYWSLEKLRYRLELMRQIYNTSDTSPELMSFVLELSSGEHSPEYPGIQYQAYARAEHQTTEQPNREAAQLLTGLASNCDVSNCLTSPTTSVSSTASSPSGSCGTASPPSPPSVIYDGSIAGSLNSGTLGKAGTRLTLANLMPSRQRLELMREMYHNEAELSPTSPDYNVESLTGGDPFYDRFPWFRMVGRSFVYLSNLLYPVPLVHKVAIVNERGDVRGYLRVAVQPVMDEENADFSNGVKQSARILFDEEQNGQHKVPKIRTIPDKDEKYIEGGNEMGTKLEELEQEDADSGRGDSSVASELHESNDQEPGEHLQPGKEFTFRVTVLQATGIAAEYADIFCQFNFLHRHEEAFSTEPVKNSGSGAPLGFYHVQNITVPVTKSFIEYLKTQPIVFKVFGHYQHHPLHKDAKQDCQITRPPPRRMLPPSIPISQPVRSPKFGPLPCPPSSTVLAKHDVLVWFEICELAPNGEYVPAVVDHSDDLPCRGLFLLHQGIQRRIRITIVHEPTPEVKWKDIRELVVGRIRNQPEPADDEDSDSCVLSLGLFPGEVLEVPGDDRSFFRFEAAWDSSLHNSALLNRVTQTGEQIYITLSAYLELDNCARPAIITKDLSMIIYGRDARTGPRSLKHLFSGQYRNPEANRLSGVYELSLRRASEAGSPGVQRRQRRVLDTSSTYVRGEENLHGWRPRGDSLIFDHQWELEKLTRLEEVGRVRHLLLLRERLGMDTTPNPTTKTEKDVCNLAQRASASPVHMVIPPSPQTPVKDQQAPAMPERELSVRETELVWKCVKLIQGRIGCKELGAEGNGSGTQASDASPGDEGCADMNASYISSNSIELCSPERVDMPNGWEAPAPAPQTQDLSLRLYVPELEEIRVSPVVARKGYLNVLEHGGSGWKKRWVTVRRPYVFIFRSDKDPVERAVLNLATAQVECSEDQAAMVKVPNTFSVVTKHRGYLLQTLGDKEVHDWLYAINPLLAGQIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W0Q9</accession>
    <name>A0A6E8W0Q9_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Myosin heavy chain</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Muscle contraction.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Myofibril</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON010147-RD">
        <property type="protein sequence ID" value="ACON010147-PD"/>
        <property type="gene ID" value="ACON010147"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009622"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON010147"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041963"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031672">
        <property type="term" value="C:A band"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032982">
        <property type="term" value="C:myosin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008307">
        <property type="term" value="F:structural constituent of muscle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007298">
        <property type="term" value="P:border follicle cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040011">
        <property type="term" value="P:locomotion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006936">
        <property type="term" value="P:muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031033">
        <property type="term" value="P:myosin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007424">
        <property type="term" value="P:open tracheal system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045214">
        <property type="term" value="P:sarcomere organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14909">
        <property type="entry name" value="MYSc_Myh1_insects_crustaceans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.820:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000036">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000008">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.530:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.360:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.4820:FF:000002">
        <property type="entry name" value="Myosin heavy chain 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000038">
        <property type="entry name" value="Myosin heavy chain muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000019">
        <property type="entry name" value="Myosin heavy chain, isoform G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000021">
        <property type="entry name" value="Myosin heavy chain, isoform G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000025">
        <property type="entry name" value="Myosin heavy chain, isoform G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000009">
        <property type="entry name" value="Myosin heavy chain, isoform G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000010">
        <property type="entry name" value="Myosin heavy chain, isoform G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000005">
        <property type="entry name" value="Myosin heavy chain, isoform I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000024">
        <property type="entry name" value="Myosin heavy chain, isoform J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.720:FF:000001">
        <property type="entry name" value="Myosin heavy chain, muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000050">
        <property type="entry name" value="Myosin heavy chain, muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.370">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.360">
        <property type="entry name" value="Myosin S1 fragment, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008989">
        <property type="entry name" value="Myosin_S1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002928">
        <property type="entry name" value="Myosin_tail"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004009">
        <property type="entry name" value="SH3_Myosin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014751">
        <property type="entry name" value="XRCC4-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615:SF27">
        <property type="entry name" value="MYOSIN HEAVY CHAIN, MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615">
        <property type="entry name" value="MYOSIN HEAVY CHAIN, NON-MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02736">
        <property type="entry name" value="Myosin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01576">
        <property type="entry name" value="Myosin_tail_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90257">
        <property type="entry name" value="Myosin rod fragments"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51844">
        <property type="entry name" value="SH3_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 14" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="6 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 14" id="KW-0505">Motor protein</keyword>
    <keyword evidence="10" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="8 14" id="KW-0518">Myosin</keyword>
    <keyword evidence="5 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0787">Thick filament</keyword>
    <feature type="domain" description="Myosin N-terminal SH3-like" evidence="17">
        <location>
            <begin position="34"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="Myosin motor" evidence="16">
        <location>
            <begin position="87"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="14">
        <location>
            <begin position="657"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1823"/>
            <end position="1842"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1920"/>
            <end position="1961"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="180"/>
            <end position="187"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010147-PD"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="20">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1961" mass="224115" checksum="60733F57ADB18692" modified="2020-08-12" version="1">MPKPPVQVGEDPDPTEFLFVSLEQKRIDQSKPYDSKKACWVPEEKEGYVLGEIKATKGELVTVALPGGEEKNFKKEQLSQVNPPKFEKVEDMADLTYLNEAAVLHNLRQRYYSKLIYTYSGLFCVVINPYKRYPLYTNRCAKMYRGKRRNEVPPHLFAVSDGAYVNMLTNHENQSMLITGESGAGKTENTKKVIAYFATIGASGKKDENAEKKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFTGSGKLAGADIETYLLEKARVISQQTLERSYHIFYQIMSGSVKGLKEMCFLSNDIYDYNSVSQGKITIPNVDDGEECLLTDEAFNVLGFTQEEKDNIYRITSAVMHMGRMQFKQKGREEQAEADGTEDGDRVAKLLGVGTDDLYKNLLKPRIKVGNEFVTKGQNKDQVTNSVGALCKGIFDRLFKWLVKKCNETLDTKQKRAQFIGVLDIAGFEIFDFNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGINWAFIDFGMDLLACVELIEKPMGILSILEEESMFPKATDQTFAEKLMTNHLGKSAPFMKPRPPKPGIPAGHFAIGHYAGVVSYNITGWLEKNKDPLNDTVVDQFKKGSNALMVEIFADHPGQSADPAAAKGGRGKKGAGFATVSSSYKEQLNNLMTTLKSTQPHFVRCIIPNEMKTAGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKLRYKILCPQLIKEPCSPEKVTQIVLTHIQLPEEQFRMGKTKVFFRAGVLGQMEEFRDERLSKIMSWMQAWCRGYLSRKEFKKMQEQRVSLEIVQRNLRKYLKLRTWAWWKLWQKVKPLLNVSRVEDQIAKLEEKATKAQEAYEKEEKLRKELEALNSKLLAEKTALLDSLSGEKGALQEYQEKAAKLTAQKNDLENQLRDTQERLAQEEDARNQLFQTKKKLEQEIGSQKKDAEDLELQIQKIEQDKASKDHQIRNLNDEIAHQDELINKLNKEKKMQGEVNQKTAEELQAAEDKVNHLNKVKAKLEQTLDELEDSLEREKKLRGDVEKAKRKVEGDLKLTQEAVADLERNKKELEQTVLRKDKEISALSAKLEDEQSLVGKLQKQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELAKLRRDLEEANIQHEGTLANLRKKHNDAVAEMAEQVDQLNKLKTKAEKERTQYFAELNDARIGCDQLSNEKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEDAESQVSQLSKIKISLTQQLEDTKRLADEEARERATLLGKFRNLEHDLDNLREQVEEEAEGKGDIQRQLSKANAEAQLWRSKYESEGVARAEELEEAKRKLQARLAEAEETIESLNQKCIALEKTKQRLATEVEDLQLEVDRASSIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKSRKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDVQSALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELSDAHEQLNEVSAQNASIAAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAESNALKGGKKAIQKLEQRVRELESELDSEQRRHADAQKNLRKSERRIKELTFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADIAEQAATKFRTKGGRAGSVQRGASPAPQRQPSAMPALAGLNLPTFDDHGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W115</accession>
    <name>A0A6E8W115_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Multidrug resistance-associated protein 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>17beta-estradiol 17-O-(beta-D-glucuronate)(in) + ATP + H2O = 17beta-estradiol 17-O-(beta-D-glucuronate)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:60128"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:82961"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:60129"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>ATP + H2O + xenobioticSide 1 = ADP + phosphate + xenobioticSide 2.</text>
            <dbReference type="EC" id="7.6.2.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>leukotriene C4(in) + ATP + H2O = leukotriene C4(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38963"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57973"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:38964"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.</text>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON009835-RB">
        <property type="protein sequence ID" value="ACON009835-PB"/>
        <property type="gene ID" value="ACON009835"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005693"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_008918"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON009835"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031323"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031768"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008559">
        <property type="term" value="F:ABC-type xenobiotic transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18595">
        <property type="entry name" value="ABC_6TM_MRP1_2_3_6_D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18603">
        <property type="entry name" value="ABC_6TM_MRP1_2_3_6_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03244">
        <property type="entry name" value="ABCC_MRP_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000230">
        <property type="entry name" value="AGAP008437-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000197">
        <property type="entry name" value="AGAP009835-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000001">
        <property type="entry name" value="ATP-binding cassette subfamily C member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000007">
        <property type="entry name" value="ATP-binding cassette subfamily C member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000812">
        <property type="entry name" value="multidrug resistance-associated protein 1 isoform X15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000074">
        <property type="entry name" value="Multidrug resistance-associated protein 5 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056227">
        <property type="entry name" value="TMD0_ABC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF443">
        <property type="entry name" value="MULTIDRUG-RESISTANCE LIKE PROTEIN 1, ISOFORM I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24357">
        <property type="entry name" value="TMD0_ABC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="12 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="68"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="101"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="133"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="170"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="296"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="350"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="426"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="451"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="528"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="585"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="612"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1006"/>
            <end position="1030"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1050"/>
            <end position="1079"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1358"/>
            <end position="1378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1447"/>
            <end position="1465"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="19">
        <location>
            <begin position="313"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="18">
        <location>
            <begin position="675"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="19">
        <location>
            <begin position="1010"/>
            <end position="1133"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="19">
        <location>
            <begin position="1210"/>
            <end position="1268"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="19">
        <location>
            <begin position="1272"/>
            <end position="1500"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="18">
        <location>
            <begin position="1537"/>
            <end position="1771"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="924"/>
            <end position="976"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="954"/>
            <end position="964"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009835-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1776" mass="199005" checksum="EF6B2D3F3824AC2D" modified="2020-08-12" version="1">METHPMDEFCGSRFWDLNLTWYTDDPDLTPCFQQTVLVWAPCAFLWLFSFLELYYLRKSANKDVPWSFVNVSKLLVIGSLIVLTIVDLAKAISTDGISAPLFYYTPVIKLASFIFVAILIYLNKHYGMRTSGLLFLFWFLLTVCSIPRVRTEIRAYEARVVEDSWAEYQFVSFLIFFSLTCIMFLLNFFVDKPPRQSKYEITDKDCPELAASFPSRIFFAWFDRLAWVGFRKPLEVDDLWKMKPEDSSKEVSPAFLHHWNETLEKTYQSRETSNDVTFKKLGNSARVDFRTTKAKNIASILPALIKTFGGTFLLGSFLKLGQDLLTFASPQILKLIIGFVGGEEPMWKGLMYAITLFVVAGTQTLLLGQYFNRMFFVGLRIRTALISAIYRKALIISNSARKGSTVGEIVNLMAVDAQRFMDLTTYINMIWSAPLQIGLALYFLWQILGPSVLAGLAVMIILIPVNGVIANMIKTLQIKQMKNKDERVKLMNEVLSGIKVLKLYAWEPSFEQQILKIRDKEVKVLKSAAYLNAGTSFIWSCAPFLVSIISFATYVLSDESHVLTPVKAFVCLTLFDILRMPLVLLPVLIVYAIQVSLVTFATYVLVDENNVLDASTAFVSLSLFNILRFPLSMLPMLISNMVQTSVSVNRINTFLNQEELDPDNVQHDEKESSPLLIENGVFSWGGEETTLKNINVRVEKNQIVAVVGTVGSGKSSLLSAFLGEMDKISGRVNTLGRIAYVSQQAWIQNATLKDNILFGKPMDQRRYARVIEACALKPDIEMLPGGDMTEIGEKGINLSGGQKQRVSLARAVYNDADVYFLDDPLSAVDSHVGKHIFEQVIGPSGLLAKKTRVLVTHGITYLPNTDKIFVLREGEISESGTYQELMDKKGAFAEFLIQHLQEVSEEEEDLDEIKQQLENSVGGEELLNQLKRSNSKRSRSESTSETGSVKDISRQNSTTDSNTSLRRRTSEKAPEVAKTKLIETEKSETGSVKWEVYKHYLKSIGLTLSVATVILNMIFQGFSIGSNLWLSRWSTDDTAGNDTSRRDMYLGVYGAFGAGQVLANFVATLTFALGSLYAAKTMHELLLRYVLHWPMSLFDTTPLGRVLNRFSKDVDTVDNTLPQLIRSFLAQFFAHLPVDVVPLHWRAGSVPDAAQDAATARAARTTDLLFRHHARRPGAEPVQQGYRHDGFRSAGDPARLECLLFRVMGSIQATLILHRRLLEGILRSGMTFFDTTPRGRIIARFSNDINTLDYSLPMNIKNFIPTVLRVCVARVAFDITLFLGCWAAAVRVHKLLLTNVLHLPMDFFDTTPIGRILQRFSKDVDVLDVKLPGLLLDWIICAIEVVATLVVISISTPIFAAVIVPIGILYYAVQRFYVATSRQLKRLESVSRSPIYSHFGETIQGVQTIRAYSVQDRFILESDEKVDGNQLCYCPSIIANRWLAVRLEMVGNLIILFAALFAVLGRETMNAGLVGLSVSYALQITQTLNWLVRMTSDVETNIVAVERIKEYGETKQEAAWELPNSTLPRDWPEQGMVEFRDFQVRYREGLELVLRGISFTVNGGEKVGIVGRTGAGKSSLTLALFRIIESAGGSIVIDGQDISQLGLHALRSRLTIIPQDPVLFSGTLRINLDPFNAQSDDDIWKALEHAHLKTFVKGLTAGINHEVTEGGENLSVGQRQLICLARALLRKTKVLILDEATAAVDLETDDLIQRTIRTEFKDCTVLTIAHRLNTIMDSDKVIVLDKGQIVEFAPPAELLQSKNSAFYSMAKDAGLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W1D4</accession>
    <name>A0A6E8W1D4_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Transporter</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Unusual broad substrate spectrum amino acid:sodium cotransporter that promotes absorption of the D isomers of essential amino acids. Neutral amino acids are the preferred substrates, especially methionine and phenylalanine.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8W1D4"/>
    <dbReference type="EnsemblMetazoa" id="ACON010865-RB">
        <property type="protein sequence ID" value="ACON010865-PB"/>
        <property type="gene ID" value="ACON010865"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012223"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON010865"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031446"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005283">
        <property type="term" value="F:amino acid:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0089718">
        <property type="term" value="P:amino acid import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10324">
        <property type="entry name" value="SLC6sbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000175">
        <property type="entry name" value="Na/ntran_symport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037272">
        <property type="entry name" value="SNS_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF037979">
        <property type="entry name" value="Na_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616:SF321">
        <property type="entry name" value="SODIUM-DEPENDENT NUTRIENT AMINO ACID TRANSPORTER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616">
        <property type="entry name" value="SODIUM/CHLORIDE DEPENDENT TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00209">
        <property type="entry name" value="SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00176">
        <property type="entry name" value="NANEUSMPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161070">
        <property type="entry name" value="SNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00610">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50267">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 14" id="KW-0915">Sodium</keyword>
    <keyword evidence="12" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 16" id="KW-0769">Symport</keyword>
    <keyword evidence="4 16 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 16" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="88"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="118"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="160"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="254"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="284"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="326"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="368"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="427"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="464"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="493"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="538"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="572"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="337"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="436"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="440"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="199"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00037785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR600175-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR600175-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU003732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010865-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="636" mass="70319" checksum="1FF0377A7B2AFDB7" modified="2020-08-12" version="1">MEGRDNNGFIGDNSPSIAGQYRWTTPAAPNEVHVTHGGLALTDVELAVGGKTVPRPTPATGEDAGRVPTTIIPEPNADRDQWGKGVEFLMSCIAMSVGLGNVWRFPFVALENGGGAFVIPYIIVLLLVGKPVYYMEMIIGQFSSRGSVKVYDMAPIMRGVGYGQLFSVTALITYYSSLMALIARYMIDSFMNPLPWAHCRSEWQPNCIDSVAGSASPNDSSSNRTLTSSSELYFTKVVLKELDGIDDGIGLPDLRLTLFLVLSWSLVFLTLIKGVKSSGKASYFLALFPYVVMTVLLVRACTLPGAVDGIVYFLKPQWDKIYDPKVWYAAVTQCFFSLSICFGNIIMYSSYNKFRHNVYRDATIVTSIDTFTSLLAGCTIFGILGHLAHVTGKTDVGNVVKSDAGLAFISYPEAIAKFEVLPQAFSVLFFLMLFVLGIGSNVAMTSCVMTVIKDQFPRVRNWQAATIIAICGVLLGSIYVTPGGQYVLKLVDYYGASSIALVLAIAELIAIGWVYGVDRLCKDTEFMLGHRPNLYWRLCWRWITPLLMFVILIYNLVTLEPLMYKQYVYPTVAYGIGWCIFAFGLLQLPIWAAYAVYKQSGKSLNEKIKNAFKPTAAWGPIDPATHYEYKKFIDED</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W1E0</accession>
    <name>A0A6E8W1E0_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="9">MFS_1_like domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. MFSD6 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8W1E0"/>
    <dbReference type="EnsemblMetazoa" id="ACON010048-RB">
        <property type="protein sequence ID" value="ACON010048-PB"/>
        <property type="gene ID" value="ACON010048"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000020"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON010048"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037088"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd17335">
        <property type="entry name" value="MFS_MFSD6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024989">
        <property type="entry name" value="MFS_assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051717">
        <property type="entry name" value="MFS_MFSD6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16172">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 6-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16172:SF41">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 6-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12832">
        <property type="entry name" value="MFS_1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="22"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="329"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="370"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="403"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="444"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="474"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="507"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily associated" evidence="8">
        <location>
            <begin position="2"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="149"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="640"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="728"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="853"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="153"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="179"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="640"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="853"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="874"/>
            <end position="883"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010048-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="883" mass="98015" checksum="F329F1D01E2B7687" modified="2020-08-12" version="1">TSSLLPYLTIHMQSTGLTVEEIAIIYLALPFTTFLSPPITGFLVDKFGRYKPVVIMSLLLNALFHHSLLLIPQQEIPGKLPEAYVMRHPGTGNVEVWWSPCPSRECPEEEEIDIVVDQCLDHCLLQEQNPKIELAPPPTDLPLVVTPIGDEPNDPKDDLDLYVRNKGKGNKNKTADVLSSTSSSSSTTELYMDKSLLKETPEESEESEEGWVVAGSGDSAFIVLDMHPDLGEPIEQLGMEIEHDDNATVTDFKTRFGVALLWQQGVNVTALEEEDLRCGGLVMTSNMTLNSNIKLSEWAADCMVQRCRFRRNGPDICPPDYKESDDKIFWIYFLLRFLATTMLSAGVTIMDPIALTMIEKYGGDFGRERLFSSIGMAIFSPITGVLIDLFSKDLGYTDYSAAFYTYDILLIISSVTVFLMPLGEKLPADNVFKDLLNLLKLKHVIIFIWFLFLLGNFWGFIESFLFLYLKELGAPNYLLGITITVGTVSSIPFLYGAGRITKIVGHVNLIVIAFIAHACRLVGYSFIENAWWCFPFEAMEALSCHLMWVAAATYCALLAPKNLLATLIGVLGMAHFSLGRGSGSFFGGFLIGEVGTREAFRYMGLVAVAGGLAYKFIHSIWLRKYDNPDADDEAVEKGEAEKLYTDGDEPKTKDQGTSMSQERLSLMIKYNQIGSLTSLPRGSRGDVNDVFSKRRSSYNIEFVRAQKGGGSASKVDLLKSAVDINHKASHQHLRKEGKASDQSLNSKRADSAPRLNQSKNISQPILSAVVDEDLPSSVLSRHRKKHHQSGILVVKACSQDGDLDLRNYLRETEQRDSITEGEEENYKLELSKKASRGHVLNDIPEKTVEAVDTAAATPPVTPVVEGTDTTTTTTHHHHSEHSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W1E2</accession>
    <name>A0A6E8W1E2_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Arginyl-tRNA--protein transferase 1</fullName>
            <shortName evidence="5">Arginyltransferase 1</shortName>
            <shortName evidence="5">R-transferase 1</shortName>
            <ecNumber evidence="5">2.3.2.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Arginine-tRNA--protein transferase 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in the post-translational conjugation of arginine to the N-terminal aspartate or glutamate of a protein. This arginylation is required for degradation of the protein via the ubiquitin pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>an N-terminal L-alpha-aminoacyl-[protein] + L-arginyl-tRNA(Arg) = an N-terminal L-arginyl-L-aminoacyl-[protein] + tRNA(Arg) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10208"/>
            <dbReference type="Rhea" id="RHEA-COMP:9658"/>
            <dbReference type="Rhea" id="RHEA-COMP:9673"/>
            <dbReference type="Rhea" id="RHEA-COMP:10636"/>
            <dbReference type="Rhea" id="RHEA-COMP:10638"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78513"/>
            <dbReference type="ChEBI" id="CHEBI:78597"/>
            <dbReference type="ChEBI" id="CHEBI:83562"/>
            <dbReference type="EC" id="2.3.2.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the R-transferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.8" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8W1E2"/>
    <dbReference type="EnsemblMetazoa" id="ACON010373-RB">
        <property type="protein sequence ID" value="ACON010373-PB"/>
        <property type="gene ID" value="ACON010373"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002895"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON010373"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038741"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004057">
        <property type="term" value="F:arginyl-tRNA--protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017137">
        <property type="entry name" value="Arg-tRNA-P_Trfase_1_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030700">
        <property type="entry name" value="N-end_Aminoacyl_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007472">
        <property type="entry name" value="N-end_Aminoacyl_Trfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007471">
        <property type="entry name" value="N-end_Aminoacyl_Trfase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21367">
        <property type="entry name" value="ARGININE-TRNA-PROTEIN TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21367:SF1">
        <property type="entry name" value="ARGINYL-TRNA--PROTEIN TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04377">
        <property type="entry name" value="ATE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04376">
        <property type="entry name" value="ATE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037207">
        <property type="entry name" value="ATE1_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="N-end aminoacyl transferase N-terminal" evidence="7">
        <location>
            <begin position="15"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="N-end rule aminoacyl transferase C-terminal" evidence="8">
        <location>
            <begin position="262"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="149"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="149"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="172"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="189"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR037207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010373-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="477" mass="54690" checksum="1542245028A5D068" modified="2020-08-12" version="1">MSYSVVEYYGENSKYQCGYCKQSSSCSSYGMWAHKLSCQDYQELIDRGWRRSGCYCYKPMMDVTCCPSYTIKCDAINFRLNKSHKKIIKRMNKFLRDGLKETSEQDAQSNIDEIATHDGSACDQEHTEVPSMQPRVLDPDLLNTLTHVNSEKRSPSADDKTSALPIPPLSGVVNTSTHTSDSSISNQPKKAKLRRIERKREKLQKKGLSGPDIEQLMQSNNRKSAEKSLEEFLSESPQDNDSPAHRLKVKQVNANDGATAATFKLYSLYQQSIHNDPASKLSMDRFKRFLPFQGFGTFHQQYWLDDRLIAVGVIDVLPNCVSSVYFFYDPEYKFLSLGTYGSLRELAYTRSLYKEYPSISNYYMGFYIHSCPKMRYKSNLQPSYLLCPEAYTWHLLDRTVVAKLDASKYSRLNDDPTAQDTNKATEQDVNDVLLIFGRSCMTYTQYLTVVGKELPILFEYARLVGKSCAKKMMLYRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W2R5</accession>
    <name>A0A6E8W2R5_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Superoxide dismutase [Cu-Zn]</fullName>
            <ecNumber evidence="8">1.15.1.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Destroys radicals which are normally produced within the cells and which are toxic to biological systems.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>2 superoxide + 2 H(+) = H2O2 + O2</text>
            <dbReference type="Rhea" id="RHEA:20696"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:18421"/>
            <dbReference type="EC" id="1.15.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
        <text evidence="8">Binds 1 copper ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the Cu-Zn superoxide dismutase family.</text>
    </comment>
    <dbReference type="EC" id="1.15.1.1" evidence="8"/>
    <dbReference type="RefSeq" id="XP_040232732.1">
        <property type="nucleotide sequence ID" value="XM_040376798.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8W2R5"/>
    <dbReference type="SMR" id="A0A6E8W2R5"/>
    <dbReference type="EnsemblMetazoa" id="ACOM042697-RA">
        <property type="protein sequence ID" value="ACOM042697-PA.1"/>
        <property type="gene ID" value="ACOM042697"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON010347-RB">
        <property type="protein sequence ID" value="ACON010347-PB"/>
        <property type="gene ID" value="ACON010347"/>
    </dbReference>
    <dbReference type="GeneID" id="120955706"/>
    <dbReference type="KEGG" id="acoz:120955706"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_014075"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON010347"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042143"/>
    <dbReference type="Proteomes" id="UP000075882">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004784">
        <property type="term" value="F:superoxide dismutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00305">
        <property type="entry name" value="Cu-Zn_Superoxide_Dismutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.200:FF:000001">
        <property type="entry name" value="Superoxide dismutase [Cu-Zn]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.200">
        <property type="entry name" value="Superoxide dismutase, copper/zinc binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036423">
        <property type="entry name" value="SOD-like_Cu/Zn_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024134">
        <property type="entry name" value="SOD_Cu/Zn_/chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018152">
        <property type="entry name" value="SOD_Cu/Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001424">
        <property type="entry name" value="SOD_Cu_Zn_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10003">
        <property type="entry name" value="SUPEROXIDE DISMUTASE CU-ZN -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00080">
        <property type="entry name" value="Sod_Cu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00068">
        <property type="entry name" value="CUZNDISMTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49329">
        <property type="entry name" value="Cu,Zn superoxide dismutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00087">
        <property type="entry name" value="SOD_CU_ZN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00332">
        <property type="entry name" value="SOD_CU_ZN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="6 8" id="KW-0186">Copper</keyword>
    <keyword evidence="2 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Superoxide dismutase copper/zinc binding" evidence="9">
        <location>
            <begin position="13"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010347-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="153" mass="15676" checksum="A034C01A8D9C95ED" modified="2020-08-12" version="1">MPLKAVCVLNGEVKGTIFFEQSGESDPVKVTGSVTGLKPGDHGFHIHEFGDNTNGCMSTGAHFNPYAKTHGGPDAEERHAGDMGNIVADENGEAKVDLTATQIALSGALNVVGRSLVVHADPDDLGVGGHELSKTTGNAGARLACGVIGLCKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W2T9</accession>
    <name>A0A6E8W2T9_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Endophilin-A</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8W2T9"/>
    <dbReference type="EnsemblMetazoa" id="ACON010376-RB">
        <property type="protein sequence ID" value="ACON010376-PB"/>
        <property type="gene ID" value="ACON010376"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001275"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON010376"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038642"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07594">
        <property type="entry name" value="BAR_Endophilin_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004148">
        <property type="entry name" value="BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03114">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00721">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51021">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="BAR" evidence="2">
        <location>
            <begin position="29"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010376-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="363" mass="40634" checksum="BFF6104B7C7F7ECC" modified="2020-08-12" version="1">MNIKMPDFDVKKFVKGAGSTLSRVVQLTEEKLGTSEKTEMDARFEHLSERSDSAKLWTEKIMRDTEAALIPNPANRVEDFIFEKIEKQKPKRLSNLEYLGLDMIEGGGEFGQDGPYGSALIKVGQAEQKLGSCERDFIGSAGMCFIQPLKKFLEGEMKTITKEKGVLESKRLDLDACKNRVRKARSMIGQQAKDGISPEVTLEQAERDLRVAQSEFDRQAEITKLLLEGISTTQATHLRYLHSLVEAQVRYYGQCNKIMSDLQRELVSLGGPQPYVPVSGYEDEDALSERVNNIELGANSGYRRARVLCSYDAKDGTELNLSSNEAVIFICECNPPHSDYMNGKQGLLKGLVPKAFLEMLDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W333</accession>
    <name>A0A6E8W333_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Long-chain-fatty-acid--CoA ligase</fullName>
            <ecNumber evidence="13">6.2.1.3</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoAs for both synthesis of cellular lipids, and degradation via beta-oxidation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(5Z,8Z,11Z,14Z)-eicosatetraenoate + ATP + CoA = (5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19713"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32395"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57368"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.15"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:19714"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>(E)-hexadec-2-enoate + ATP + CoA = (2E)-hexadecenoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:36139"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:61526"/>
            <dbReference type="ChEBI" id="CHEBI:72745"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:36140"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>12-hydroxy-(5Z,8Z,10E,14Z)-eicosatetraenoate + ATP + CoA = 12-hydroxy-(5Z,8Z,10E,14Z)-eicosatetraenoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:52112"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:90718"/>
            <dbReference type="ChEBI" id="CHEBI:136408"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:52113"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>15-hydroxy-(5Z,8Z,11Z,13E)-eicosatetraenoate + ATP + CoA = 15-hydroxy-(5Z,8Z,11Z,13E)-eicosatetraenoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:52116"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:78832"/>
            <dbReference type="ChEBI" id="CHEBI:136409"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:52117"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>5-hydroxy-(6E,8Z,11Z,14Z)-eicosatetraenoate + ATP + CoA = 5-hydroxy-(6E,8Z,11Z,14Z)-eicosatetraenoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:52108"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:65341"/>
            <dbReference type="ChEBI" id="CHEBI:136407"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:52109"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a long-chain fatty acid + ATP + CoA = a long-chain fatty acyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15421"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57560"/>
            <dbReference type="ChEBI" id="CHEBI:83139"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:15422"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>hexadecanoate + ATP + CoA = hexadecanoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:30751"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:30752"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 13">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.3" evidence="13"/>
    <dbReference type="RefSeq" id="XP_040231527.1">
        <property type="nucleotide sequence ID" value="XM_040375593.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8W333"/>
    <dbReference type="EnsemblMetazoa" id="ACON011603-RB">
        <property type="protein sequence ID" value="ACON011603-PB"/>
        <property type="gene ID" value="ACON011603"/>
    </dbReference>
    <dbReference type="GeneID" id="120955083"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009658"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON011603"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032488"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047676">
        <property type="term" value="F:arachidonate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05927">
        <property type="entry name" value="LC-FACS_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045311">
        <property type="entry name" value="LC-FACS_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272:SF107">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 13" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2 13" id="KW-0436">Ligase</keyword>
    <keyword evidence="13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="3 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="14">
        <location>
            <begin position="114"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU369030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON011603-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="692" mass="76929" checksum="9027D57A40BEF856" modified="2020-08-12" version="1">MLKFIKTCVDEIEENLQYVGGTAGAVALTTAVAAATYYYSTRPVPEKPLVPLDNQCPIEAGPEQVHVSKFYKEAKDGKFVSCLEEDIRTLYQSFRKGAYTSNNGPCLGWRENLQSPYQWMNFNETLLRAKNFGSGLISLGLRPGPSTFIGIYSQNRPEWVLFEQGCYCYSLVVVPLYDTLGPDACAFIVDQTEMQVVVVEDDKKVNLLLDKAPRSLRKIVAIKEVRPATLQRAKNVGIDVHTFDEVEKLGAISDNPELEPKPDDLCTVCYTSGTTGNPKGVMLTHANVLAGVAGVLLQLGPHRPRVGDIMVSFLPLAHMLERCCENGVYYMGGAVGFFSGDIKELTNDLKALKPTLMPAVPRLLNRVYDKVFAEVQRSSVKKLLLNMALSSKEAEIKRGIVRKNSIWDKLVFRKIQEGFGGRLRLMVVGSAPLSEAVLSFSRAALGCLICEGYGQTECTAPITLTVQGDFVAGHVGPPVACNGIKLVDVPEMEYYASQQQGEICVKGANVFIGYLKDPQRTAETIDKDGWHHTGDIGQWLPNGTLKVIDRKKHIFKLSQGEYIVPEKIENAYIRSQYVEQVFVHGESLKSCIVAIVVPDVEVIKSWAQENNIPGTLSVLCNNPDVKQLILNDMILWGKEFGLKSFEQVKDIYLHPDPFSVQNGLLTPTYKSRRPQIRSYFAPQLDDMYKHLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W3H6</accession>
    <name>A0A6E8W3H6_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Potassium channel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the two pore domain potassium channel (TC 1.A.1.8) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8W3H6"/>
    <dbReference type="EnsemblMetazoa" id="ACON010806-RB">
        <property type="protein sequence ID" value="ACON010806-PB"/>
        <property type="gene ID" value="ACON010806"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007641"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON010806"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_029834"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015271">
        <property type="term" value="F:outward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022841">
        <property type="term" value="F:potassium ion leak channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030322">
        <property type="term" value="P:stabilization of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003280">
        <property type="entry name" value="2pore_dom_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11003:SF335">
        <property type="entry name" value="POTASSIUM CHANNEL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11003">
        <property type="entry name" value="POTASSIUM CHANNEL, SUBFAMILY K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01333">
        <property type="entry name" value="2POREKCHANEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5 8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="157"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="255"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="288"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="412"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="441"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="467"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="11">
        <location>
            <begin position="234"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="11">
        <location>
            <begin position="422"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="354"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="546"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="354"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="366"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF07885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010806-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="568" mass="62949" checksum="492D4F4A3E67B37B" modified="2020-08-12" version="1">MTQATDSRTPPAPNAAGTRPRINLTMPPVYVAPPSVQAQHTPAKYSPAHYFQQLHQQQQQQAAQMLHNPLYQPGVPVTPGSPTRISLGTDGGLGAGGEQPDYTDYYSMYPAKAGEFMFKQFEGFRDFTLNTAKSGLGVGEKSVFWMYTKITKWSRKWFTHFFLFLILFLYSVAGAALFVAVEGTHEKKMETNVNHAKRMLAKNLRILALDREKLENPDPDIWEGRAINLVEEYSAVLYQSYKEGSFENKQNKKTWSFWNAVFYCGTIYTTIGYGHISPSTTTGRALTILYAIIGIPIFLIVLADFGKLFTRGIKFMWAYVRRLYYTGSVKKVRKTAQVQVSLKPADNELQGATTTTTVAAQQPQQSTPPPPVQRPPSEAPTAAASGTGIEAIPVPDTPTTPVPETFEIDDEFNLPISVAIVILVAYMLFGAQIYCTWENWSFFEAFYFVFISISTIGFGDYVPQHPIYMMCSILYLIFGLALTSMCINVVQLKLSDSFRQASAKIGATIGLQMAEAASQHQHSPVHTPIELAAVHTSTPTTAVNVSLKDTPTLPARPSSSVPKPDKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W590</accession>
    <name>A0A6E8W590_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GDT1 family protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the GDT1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_049466294.1">
        <property type="nucleotide sequence ID" value="XM_049610337.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8W590"/>
    <dbReference type="EnsemblMetazoa" id="ACON011962-RA">
        <property type="protein sequence ID" value="ACON011962-PA"/>
        <property type="gene ID" value="ACON011962"/>
    </dbReference>
    <dbReference type="GeneID" id="120959978"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_013639"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON011962"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031799"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015085">
        <property type="term" value="F:calcium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005384">
        <property type="term" value="F:manganese ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032468">
        <property type="term" value="P:Golgi calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032472">
        <property type="term" value="P:Golgi calcium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001727">
        <property type="entry name" value="GDT1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049555">
        <property type="entry name" value="GDT1-like_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12608:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 165"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12608">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN HTP-1 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01169">
        <property type="entry name" value="GDT1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01214">
        <property type="entry name" value="UPF0016"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="116"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="146"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="230"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="268"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="300"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="196"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="210"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
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    </evidence>
    <sequence length="332" mass="35513" checksum="A40C30081438B037" modified="2020-08-12" version="1">MSGKSVMNKFVLRNIAQRLVHCMYVCVFYLILVCMLDTAGIGADKMPQEDISVVTEVDGLSPDGSSSAPTEGKGLLSSDIGFVHAFAASFMVIIVSELGDKTFFIAAIMAMRHPRLTVFAGAIAALALMTVLSVLFGIAATIIPRVYTFYISTALFALFGLKMLYDGYHMSATGAAEELEEVQSDLRKREDELEREANAGGSLMSQDAESGASTSGRRGTGRGRNSNNSALKLLLRIFMQAFTMTFVAEWGDRSQLTTVILSARENVYGVIAGGIIGHSICTGLAVIGGRMIAQRISVRTVTLIGGVVFLLFALSALIFGPGEEEPVKVPIT</sequence>
</entry>
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                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
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            <dbReference type="PubMed" id="30669388"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain>Mali-NIH</strain>
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            <strain evidence="2">Ngousso</strain>
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</entry>
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                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
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                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
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    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON029667-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1703" mass="189240" checksum="DB03FF499D3BBA5D" modified="2020-08-12" version="1">MCENSESSMEELINNEDNSSAKATSINTSTTVSLPTGAILGNNGHNGEEISLSKKAIHQRDADENLFLRFLELDPSPDATSNNAIVGQQVPTTPRRSSISTCGPSKPTAGTTNSSGRTPFTITKRLTRMGDRGFGFSIVWTHPPRVEKIETGLSADRAGILPGDYVVFVDKHNVVTMPEQDVLNLIRTQGNTLLLEIFRRPQSASGPQLSNRTNGVRNISSGQTSISNHGGMINSTGNITSFNVSSSSAHVQADDGEPFASPSPFGVARSSTACSNTSIEIAKRKLHLPQVTFSKESIVQQYPDDHRRKFLYQLISREQHFINAINFGIERFVNPLRERKDLISPNDHKILFQNIDELSRISEDILEQIIQDDTEPQIHFASRVYLSKNTALCAAYRKYCNGLKKADCVLVNKSRNSICDFMKFITEPPVPRKRPDLTTFIHRPLQHFREILKLVQMIASHCRVDSEEHANFNNIISELQIAYREITVGGGLMEPIGEGRPLLTLQDLEARMVFTKCKPFQLASHGRQWIFGGDLSRIEGRSVKPYWTLLFSDILLFAKVSRDRVLFITEEPITLSTITDSCFNIRKKNTEFRITIDPNARQIESPTVHCAPDLTRTPKKNFKKRYISLRAPSTELKAVWQNLLTRQIFLVNATLGSTPMSSPLDSPDILQSFLPFTDIGTTVTSAASVKVGSLNSMQIKNQQSGVSKVPKRVDELIDEKCRKLNKTGVPQGSALHLAQWMKGQLNKQIIESDPIDSDPEQLVEDWSVEQVTNRSKELNLVNSDGSPFRSAPRYNGSSNGTLVKNNTINDQPFIDDNSACDDNDEDNKSVSKSTTSDSQITVRSSPLSNKLDTISMCRQCLKNCKSRFNSPSTNSLIVQQSHCTSSNHCCVSNSTTGSSLDKSINSTSSISSSSTLTMPGRQNAGCSNHVECAKKIFVTQTTENYEHKQISINDSYKTMCQIPCHCKCTAKDFEKSTLSPDNCLHERVKILENACNVSSRNVSIQQVKQNPTKMNGFPFNGFPFNPSLKERNAQESCNRDVYKIEDNVNKNDDTAWSLMSLIGLAQINPTSSLVQLDPFEVLPTIAVVPPTPDAFGNAGCIRKPMLPWTTIESNHLQLAPSTLSVTENSEVALNDEYSPDNSPEDEIVEPPYRALNPSLKRYGTISSLERVPSEDTDDNKTFSSDEDSECDIKIVTKEVYDNNTQTFLNWTARAGNFIEESRAFIDRYLGRRDNSTEEVEANNNDWEKCREKRVSEHTSSIGCDEEEAFEGETSATSGEEVWGTPTSGGENDDMQIFGSIEQTHSSPTKSSSSNTGDDDTELMMDELLMAPPMTASAVRGLLPRRKLEPLFEEDSESSESDDHSKPLSGRDNQGQATQNNPPVYADLNDSTGTGSVSTTPSSTLLAEKSETIKTTTPEENYGSVADRCYAFVQLPNIEKPCASDTAHYGDKVIATVGLSPRLEMRLALNHDILGDEDLLTYSPGPELTEILGRDLSTYHPMSEKDIIMNRIVTRVSSYMNIQAIASASTVSSTNVVESQVPTTSQAMRNGMSSLYQQNNSKMDTPILHRRKPIVPATWSSFGSVDREERTLSDLERLARREKVYCMTQLNHNASQLKRVASFNATQKQTNKLFNFLYRRNSENQVNGLSSSKENTQDNVTCSVPSRLSSPRIETVKSLDRRFWKLLSKRRRGSFQGELTTGAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WB01</accession>
    <name>A0A6E8WB01_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Microtubule-actin cross-linking factor 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON029051-RF">
        <property type="protein sequence ID" value="ACON029051-PF"/>
        <property type="gene ID" value="ACON029051"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007811"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON029051"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040800"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030056">
        <property type="term" value="C:hemidesmosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005882">
        <property type="term" value="C:intermediate filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045104">
        <property type="term" value="P:intermediate filament cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042060">
        <property type="term" value="P:wound healing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21189">
        <property type="entry name" value="CH_PLEC-like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00176">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000260">
        <property type="entry name" value="Kakapo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000275">
        <property type="entry name" value="Kakapo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000001">
        <property type="entry name" value="Microtubule-actin cross-linking factor 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.920.20:FF:000001">
        <property type="entry name" value="Microtubule-actin cross-linking factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1290.10:FF:000014">
        <property type="entry name" value="microtubule-actin cross-linking factor 1 isoform X21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000110">
        <property type="entry name" value="Short stop, isoform AA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000031">
        <property type="entry name" value="Short stop, isoform J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000022">
        <property type="entry name" value="Short stop, isoform K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000030">
        <property type="entry name" value="Short stop, isoform K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000044">
        <property type="entry name" value="Short stop, isoform K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000045">
        <property type="entry name" value="Short stop, isoform K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000051">
        <property type="entry name" value="Short stop, isoform K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000053">
        <property type="entry name" value="Short stop, isoform K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000054">
        <property type="entry name" value="Short stop, isoform K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000055">
        <property type="entry name" value="Short stop, isoform K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000058">
        <property type="entry name" value="Short stop, isoform K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000063">
        <property type="entry name" value="Short stop, isoform K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000064">
        <property type="entry name" value="Short stop, isoform K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000066">
        <property type="entry name" value="Short stop, isoform K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000068">
        <property type="entry name" value="Short stop, isoform K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000082">
        <property type="entry name" value="Short stop, isoform K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000045">
        <property type="entry name" value="Short stop, isoform K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000038">
        <property type="entry name" value="Short stop, isoform N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000039">
        <property type="entry name" value="Short stop, isoform N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000040">
        <property type="entry name" value="Short stop, isoform N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000042">
        <property type="entry name" value="Short stop, isoform N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000047">
        <property type="entry name" value="Short stop, isoform N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000050">
        <property type="entry name" value="Short stop, isoform N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000057">
        <property type="entry name" value="Short stop, isoform N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="29"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.920.20">
        <property type="entry name" value="Gas2-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1290.10">
        <property type="entry name" value="Plakin repeat"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041615">
        <property type="entry name" value="Desmoplakin_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003108">
        <property type="entry name" value="GAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036534">
        <property type="entry name" value="GAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049538">
        <property type="entry name" value="PCN-like_spectrin-like_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043197">
        <property type="entry name" value="Plakin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035915">
        <property type="entry name" value="Plakin_repeat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001101">
        <property type="entry name" value="Plectin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018159">
        <property type="entry name" value="Spectrin/alpha-actinin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002017">
        <property type="entry name" value="Spectrin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23169">
        <property type="entry name" value="ENVOPLAKIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23169:SF23">
        <property type="entry name" value="SHORT STOP, ISOFORM H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02187">
        <property type="entry name" value="GAS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17902">
        <property type="entry name" value="SH3_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00435">
        <property type="entry name" value="Spectrin"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21019">
        <property type="entry name" value="Spectrin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21020">
        <property type="entry name" value="Spectrin_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00243">
        <property type="entry name" value="GAS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00250">
        <property type="entry name" value="PLEC"/>
        <property type="match status" value="31"/>
    </dbReference>
    <dbReference type="SMART" id="SM00150">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="35"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143575">
        <property type="entry name" value="GAS2 domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75399">
        <property type="entry name" value="Plakin repeat"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46966">
        <property type="entry name" value="Spectrin repeat"/>
        <property type="match status" value="32"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51460">
        <property type="entry name" value="GAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 8" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="12">
        <location>
            <begin position="134"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="11">
        <location>
            <begin position="785"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="13">
        <location>
            <begin position="8230"/>
            <end position="8265"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="13">
        <location>
            <begin position="8266"/>
            <end position="8301"/>
        </location>
    </feature>
    <feature type="domain" description="GAR" evidence="14">
        <location>
            <begin position="8306"/>
            <end position="8378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1375"/>
            <end position="1402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1419"/>
            <end position="1439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="2873"/>
            <end position="2938"/>
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        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
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                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
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        </source>
    </evidence>
    <sequence length="50" mass="5619" checksum="D33AA64F9D6F49C4" modified="2020-08-12" version="1">MKLLMKITIFITLVTEKGIFRMAKLLDPVTEIIDSKCVYEVGRGPGNCMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WB31</accession>
    <name>A0A6E8WB31_ANOCL</name>
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        <submittedName>
            <fullName evidence="2">Serine-rich adhesin for platelets</fullName>
        </submittedName>
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        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
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    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="EnsemblMetazoa" id="ACON029561-RD">
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        <property type="gene ID" value="ACON029561"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012835"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON029561"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042479"/>
    <dbReference type="Proteomes" id="UP001105220">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="23"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="164"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="236"/>
            <end position="256"/>
        </location>
    </feature>
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            <begin position="319"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="879"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="965"/>
            <end position="1032"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1073"/>
            <end position="1103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1118"/>
            <end position="1147"/>
        </location>
    </feature>
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            <begin position="1238"/>
            <end position="1456"/>
        </location>
    </feature>
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        <location>
            <begin position="24"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="110"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="172"/>
            <end position="192"/>
        </location>
    </feature>
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        <location>
            <begin position="892"/>
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        </location>
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        </location>
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            <end position="1353"/>
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    </feature>
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            <begin position="1404"/>
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        </location>
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        <location>
            <begin position="1441"/>
            <end position="1450"/>
        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
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        <source>
            <dbReference type="EnsemblMetazoa" id="ACON029561-PD"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1456" mass="155341" checksum="CDF01559AC3B2011" modified="2020-08-12" version="1">MRNNHIDFVALDNILKSVADQPCTAATTPPHSNPTHSKSPPKASFLTAESLREIRGRLRRLSNESLYRDDMSPADDASGQTVASDRPSPADEGGVTITEVETRLGQLETSSSSDSNYRSSSLQTSKKPAPDTTVLAPDDWHSRRKSYGFERMSQQPLGSGFAMAKMDSSTDSGIGRSSDLSSSWSSAATESGQGAGGSYHHQRATIVTLGERSQLKEKQATNPAMVIKITSKPADSTSDTFAVPSLEPTDEEPKRHSIAVDETSYVRDSLRTMFERKSSVHVNGFSHTLIDDLSRNKKRVEFCKTEVHFTADSGRVNIVETDEKPPPTNNFRRRRRSSGGSSYLVSNLDGDSDSVNATDESKLVGPSSESVVIEQDTAPVVTTTIGGMGRTVPEPTPRKYVPSATVTVSDESDGHSTDEISLRSILKNKPVKPKPYVLGENLDSNEALWGVKLKPVVAGGATVEQSQPRLLSVEMGTESKSSSPIAAGSGYSTRINLTKEPAGPSSWSGSNSTGAQVDKEILHSATNGHESAAAKSTTKILIDMTSAGEEKGPAAAAGDELKSTSLIMRTMRAATKLDEALKSLQAFRRDSLGESGTPTGLYQRGGVDIASPKVRRHSAFCQLNGGAGVSQNSSSSSTISSHSIAKSSSASSIYADYPKLRTSGKYASSLDGDSLERDATVYGVSRYTSVLPSTATTAASRRILTSESRLSDFEAYISKNTASVRRSSTADDVDRLVSSKPIAAPRLKKLQGSTVLSQQLSQLRRLYDAAELYDSDSDSAKADEEVKLYLGNLADASSSTSSFGQATELSGSWSRIKAKRNIQKFNGSSLSTVTAARDEPKDTAFSVNSSAEFDVVKPSALISRQPAKPSAVMSIELKSPTPVKKLPPASHTSSPDTSTTKITTTLNDESTAASTQQQSPKFRRYIPTTATSATTPGAVRNEERASLRLSSGARQLRENELSFFGVNPNKQQSSSPAGPTGKPPTTSTTTPNSPFQRSATLTSSFIRRSGTTTTSSTTTTTGSGDSATSKTSLWQLTNDKPDLLRHGQQAAAAVEQPKSQAVAAPRPAVIRTGSSSVATSNSVSVNSSSSSVTTSTLSSADGSPHYENLSHFALKPKAAQRSVGSSPSAGELLVQQQTAKVAESEKPAAAYDRKKDLERDERILEELTRAADEILNVVNNMSNGESMESILSELNDGEGHYRRLSTGGCTLGTIRECSTTNKIMKSRGVQVSKNIDDSLKRHHQRQSRLSSASSNESIGRRPEGNFTRSVSLRGPSTASTTAGDSLSSRSGSSRRSRAPMATETRRLVRMCSKEKLQSNASSSEDLPSTTVVEPPRRPRRTRYHSKETKDPERTSGTSNGHGTRVSSSTRSHRSERSSGSIRTVSSRHQGQSATPTTGHTAQTAVSASVSSHHSLHSASCPSSRGLKSSATNGHHQHHHSQLASATTTTSSKKHHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WB36</accession>
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        <submittedName>
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        </submittedName>
    </protein>
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        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
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            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">Ngousso</strain>
        </source>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_040384187.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8WB36"/>
    <dbReference type="EnsemblMetazoa" id="ACON029564-RB">
        <property type="protein sequence ID" value="ACON029564-PB"/>
        <property type="gene ID" value="ACON029564"/>
    </dbReference>
    <dbReference type="GeneID" id="120960175"/>
    <dbReference type="CTD" id="35097"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005660"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON029564"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_036049"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013162">
        <property type="entry name" value="CD80_C2-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45889">
        <property type="entry name" value="IG-LIKE DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR45889:SF8">
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    </dbReference>
    <dbReference type="Pfam" id="PF08205">
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    </dbReference>
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    </dbReference>
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    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
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            <begin position="32"/>
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            <begin position="453"/>
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    </feature>
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        <source>
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        <source>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="488" mass="53162" checksum="76A7CF7934BBEC79" modified="2020-08-12" version="1">MQPKMQAVHFLPRCVLLLSVCAAFTATTTHAQVVDTEPRSITVVENQENVELHCRIGRPAQLCLIRMPGLNDQIALSPTEKSPVAGYHYHGEGLDKGSCGVRIDRVTADNAGLMNCTLFVDGRGLTGSIEIVVAFPPQQPVIEVVQGNLANVEVNSQLMFRCTAERGNPAANLSWFLDQEQIYEGVQFPEAPEEYYDERSNKHMFTATSTLKRAVRAEDNGKRLTCQAQHLAYPEGVSRTDLILDVNFQPQALPDQVVYGLQLGRTATASMVIKANPSPHVMWNIDGTDYHQGTEQGRYAVPIPEALGNNRYNVSLTIAGLTLEDLSKTYVLRASNNFGVEEYRLTLRSQDEVFSESSSFGTGEIVGLVLAVVIVLLAVALIVGARATGRWCFRGASLNTDINPDSEAQIAPHPHDDEIDERLQTQDPAAHYVHEGDERHAAAATNGKHENGKQAAKQQPVAAPIVPPPAAAKQENGKPADSKTNTSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WB37</accession>
    <name>A0A6E8WB37_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific protein-tyrosine kinase</fullName>
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        </recommendedName>
    </protein>
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        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
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        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
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            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
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            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
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    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
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                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.10.2" evidence="1"/>
    <dbReference type="EnsemblMetazoa" id="ACON029813-RA">
        <property type="protein sequence ID" value="ACON029813-PA"/>
        <property type="gene ID" value="ACON029813"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007652"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON029813"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030924"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="F:non-membrane spanning protein tyrosine kinase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="term" value="F:protein serine/threonine kinase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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    <dbReference type="GO" id="GO:0009792">
        <property type="term" value="P:embryo development ending in birth or egg hatching"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:morphogenesis of an epithelium"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="Tyrosine-protein kinase pr2"/>
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        <property type="entry name" value="SH3 Domains"/>
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        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
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        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
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        <property type="entry name" value="TYROSINE-PROTEIN KINASE"/>
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        <property type="entry name" value="PBD"/>
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        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Protein kinase-like (PK-like)"/>
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        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="126"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="12">
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            <begin position="392"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="domain" description="CRIB" evidence="14">
        <location>
            <begin position="481"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="88"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="692"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="787"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1207"/>
            <end position="1242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1309"/>
            <end position="1338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="694"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="787"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="801"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="1218"/>
            <end position="1228"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011903"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
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            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
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    </evidence>
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        <source>
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        </source>
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    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON029813-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1445" mass="159996" checksum="F34C99DDACC1049F" modified="2020-08-12" version="1">MWSAAPIMEPKVPDLYEFLAEAELQQYYNSLKNELKITNVTHLKYATDEDLRQVSGLSRPEIRRLRKYYEKYYPHGYLNKIRRLLQTPRKDENNPNSPTSSVEPIKACPSPSKIPNNKHIIPPDAICVNKQLGTGEFGIVQQGVWTNGSERIQVAIKCLCRERMQSNPMEFLKEAAMMHSIEHENIVRLYGVVLDTESLMLVTELAHLRSLLECLKDPGLRVSFLTVPTLCEFASQICNGMMYLENKRFIHRDLAARNILVFSKNKVKISDFGLSRALGVGKDYYQTNFNVNLKLPIAWCAPECINFLRFTHASDVWAFGVCLWEIFSYGFQPWAALTGHQILEAIDEPHYQRLEKPECCPKEYYALMLKCWQHDAAKRPKFSDIFLMLPDIKPEQLKTIVAYSEHKKDYLMYRQGEIITVLDKNNNAPYWKGVLNSGKTGLFNPANTVAYLDSLPSSVNRDNFCRTAERSSKRKIRTDMISRPQNDLKHTGHVGIDGVSFGDVAFLSSPQNLPRQIVTPYKPSEDLEQTPLLLPPTPTSPDSIQTASGYFSETLQLAGHQSFINAGENHHHIIDHGGTASTVVDGLTTSAFDFRNSGFKDSNPFINADDNGDNAAAYNNTAVATSSRDGQNQHEYHEISDDDIATEKVFDQGPSLMDEMEHVFKSMSTISEQGVPLSPDAENTNMRNELAELSSKMSRKTSSSTSAGTLVSKGSKAKPKKASTVKPISMKDEKLLNQVIEMANEISAKSMTDLVTDANHQTLSPKRKFSFRFPHIHNVVGGDGLRGSSNSSIAGNQPHVSGLSAGSSSHAGRGLQDKDHGILSGAGQHHPHSSNTLTNSYREKRNFSQEVNNVPDLQSTITEEARIAYNSLVENPCTSGASGGFSNSVGQGGNSSFTCSTLPRAKLTNSLATASFRFKDIDCCSPPSEPAPKSPEEADLLLSEGSMCDNITAECNPLRMMRNKNFSIVPRSKNKISTHQVPWEEQLDTASGSTNQAESPSYLTNPEYTYNNALTSTLTETRTAAEGTDNPIPLPPRDRNKTIQVNQKRHVRKYPLIIPAAGLQRTLNKVTQVTPIDEKSDVFSITDTAEPTTAANNIHDDHLRNDPDECSNLDVLHKSIESIKLGEIDEALTDLPSNATEQLAQQKGYSKEFLQNNFPSVSANPLPMVCGEQHSSTLSNTITTTTLPATIPAASAILTRRRTASDASVLGASAQRRQPPPPPPPPAPLMLSSIAPESESRDPTYENLDIFQTHNSFCIDTASLHCESILENDADACPTPHGIGGSTAATDLVDGFEASIVFDSINSSEFHQTRQPIKPPSAENEQHDRTTQPTTEGELKKSMNFVSCEDLLEFAEKPKGRARGLESDEVRIMSKVLGKKPSPAQCLLTLEFIDWDIHKAIKLCKLQTILESFNLSLQECSDALQMYDWDLHTTALKLKSGNSSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WB41</accession>
    <name>A0A6E8WB41_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Tensin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PTEN phosphatase protein family.</text>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON029568-RB">
        <property type="protein sequence ID" value="ACON029568-PB"/>
        <property type="gene ID" value="ACON029568"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012720"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON029568"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_043036"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01213">
        <property type="entry name" value="PTB_tensin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09927">
        <property type="entry name" value="SH2_Tensin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013625">
        <property type="entry name" value="PTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006020">
        <property type="entry name" value="PTB/PI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035012">
        <property type="entry name" value="Tensin-like_SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014020">
        <property type="entry name" value="Tensin_C2-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033929">
        <property type="entry name" value="Tensin_PTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051484">
        <property type="entry name" value="Tensin_PTEN_phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45734:SF10">
        <property type="entry name" value="BLISTERY, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45734">
        <property type="entry name" value="TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08416">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10409">
        <property type="entry name" value="PTEN_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00462">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01326">
        <property type="entry name" value="PTEN_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51182">
        <property type="entry name" value="C2_TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0727">SH2 domain</keyword>
    <feature type="domain" description="C2 tensin-type" evidence="8">
        <location>
            <begin position="292"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="7">
        <location>
            <begin position="1269"/>
            <end position="1375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="40"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="120"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="501"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="569"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
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            <end position="753"/>
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            <end position="863"/>
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        <location>
            <begin position="1123"/>
            <end position="1199"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="42"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="501"/>
            <end position="510"/>
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        <location>
            <begin position="522"/>
            <end position="536"/>
        </location>
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        <location>
            <begin position="569"/>
            <end position="581"/>
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        <location>
            <begin position="621"/>
            <end position="636"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="644"/>
            <end position="658"/>
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    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="704"/>
            <end position="716"/>
        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1053"/>
            <end position="1062"/>
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        <location>
            <begin position="1187"/>
            <end position="1199"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00007881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS50001"/>
        </source>
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        <source>
            <dbReference type="PROSITE" id="PS51182"/>
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            <dbReference type="EnsemblMetazoa" id="ACON029568-PB"/>
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        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1544" mass="171226" checksum="18EF35AE396951B4" modified="2020-08-12" version="1">MIRVNSQSFSLSSTLKKKNGREEEQQTINKYNSLPLHTINAQQQQQQQPRPTTNLNHVNNNHYHNYSLQPYYNRQQQQHHQQQQQLQHHSYPVARIDEEPAAQSLRLNYVTERILASTLPARRLQNGSSSPHAPNGTPPVDEHERELINMLEQKHKQNYRILDLEARLVNITLEKLCELCKYIDSWLGSGKEKIVVLQDREDKHRLGTAIAAYLQYQKICGSNIPQASGHSKNSLSPGGTDNGRRTPTWLDLDIYSMQKFLETVAGPLRIPSHRRYIQYFSGLLSGVIKLNSSSFFLKAIRVESPPCLHYRAITVNSEWRSFIKIFEGVRCLFTSDIYVIPITTRHFIYEIKHPLRLRGDILVRCYQIIPNNNKATYEKELIASVQFHTCAITEKEVQFTKGDVDFACEDERFSAEHRMTFCFDTVPNERPMILVFQDPLVRIEPIAENGDLLETINEDESGHTQGPLDGSLYATILKSPKSPTATTHLISPPAEFSNGSSKSLLLLNGNGPPPPVPERSKTPNSIYLSQNGTPRSTPVPFALAPPPAASPAFGDRSVRAVSSASNSVSVNSSYSSYQSASPGVATVPDGGSPGATLATPGGTKARPPSAQQVDGRESVRSPLTVSMDSGISSSGPVNRRVQGSSVSPSSFPSPQASPQDDRHRELDDLLSDMMLTVQGIPDVGQKADQTDHHQSQQPQQQQQHQHHHHHHHHHHQNPNDHFVNTNTDTIKRSHSAQLPQREDARSRTPLTAVSDYSPATAAAAAAKERELLMYETSSTTTTLTPPPSESGRETPLLSGPSYAAAAAAGTIRERELQNINNNNLTPAERELIMSVQRHQHQSLAYPRPARSTTGASERFTSDDDDDLLVHGGTGSVAQIPYHAREDSRPFTYGNIPATGTPQQPPAATMLKMQSGLSSPSMVRKALGTPTASRKTAGTLPRNDFEEMLRERREKVLSEKYTIGDQSPGGGMMVSDTINNNSSGDGRWTYQQHHTVKTVTTQGGGGAGTPNGYPSHEPLKRSNTMDGSFGRQFSNEGISGQSWLQLQQQKLRARREQQRREHSNSFSYNYGSPAFPTAGENAYSTTHRRSQTLSPVRNERNYHTLTTTRTHSTERPFVAVQRAHDNAKLQTIGAREESFSSYRSETEPDNSPMGGSPRPETPAFPVTPRTPYGLSNGTSSPALPPKSPTSQRRYNSAWSLRSQKSSVSTYDVSKDLFSGNQQRTQELVNQNETLSCYTSRRNSTTSNANSEPQEVAPQFVKFARDSSKYWYKPNISREEAIALLRNAAPGTFIVRDSTTFANAYGLVVKVNHPPPGVQYTGPNSEELVRHFLVEPTIRGVRLKGCANEPVFTSLSALVYQHSITPLALPCRLIIPDMDLQQMEHQTPAQQQLLQQGAACNVLYLFTCDTESLTGPQAIRKAVSSLLALRPLPKPTQVHFKASLQGITLTDNTRQLFFRRHYPSNNVSFCALDPDDRRWSIQSTTGDIPASKRMFAFVAKRSPSSADNQCHVFCELEPTQPAAAIIQFANKVLSGTSQQPAVTRAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WCS7</accession>
    <name>A0A6E8WCS7_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON029051-RC">
        <property type="protein sequence ID" value="ACON029051-PC"/>
        <property type="gene ID" value="ACON029051"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007811"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON029051"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040800"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030056">
        <property type="term" value="C:hemidesmosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005882">
        <property type="term" value="C:intermediate filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045104">
        <property type="term" value="P:intermediate filament cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042060">
        <property type="term" value="P:wound healing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="16"/>
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        <property type="entry name" value="Microtubule-actin cross-linking factor 1"/>
        <property type="match status" value="3"/>
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        <property type="entry name" value="Microtubule-actin cross-linking factor 1"/>
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        <property type="entry name" value="microtubule-actin cross-linking factor 1 isoform X21"/>
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        <property type="entry name" value="Short stop, isoform AA"/>
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        <property type="entry name" value="Short stop, isoform K"/>
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        <property type="entry name" value="Short stop, isoform N"/>
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        <property type="entry name" value="Gas2-like domain"/>
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        <property type="match status" value="13"/>
    </dbReference>
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        <property type="entry name" value="EF-hand-dom_pair"/>
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        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
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        <property type="entry name" value="EF_hand_dom"/>
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        <property type="entry name" value="GAR_dom"/>
    </dbReference>
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        <property type="entry name" value="GAR_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="Plakin"/>
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        <property type="entry name" value="Plakin_repeat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001101">
        <property type="entry name" value="Plectin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018159">
        <property type="entry name" value="Spectrin/alpha-actinin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002017">
        <property type="entry name" value="Spectrin_repeat"/>
    </dbReference>
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        <property type="entry name" value="ENVOPLAKIN"/>
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    </dbReference>
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        <property type="entry name" value="SHORT STOP, ISOFORM H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02187">
        <property type="entry name" value="GAS2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Spectrin"/>
        <property type="match status" value="15"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="GAS2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PLEC"/>
        <property type="match status" value="31"/>
    </dbReference>
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        <property type="match status" value="28"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143575">
        <property type="entry name" value="GAS2 domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Plakin repeat"/>
        <property type="match status" value="17"/>
    </dbReference>
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        <property type="entry name" value="Spectrin repeat"/>
        <property type="match status" value="25"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51460">
        <property type="entry name" value="GAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="7007"/>
            <end position="7042"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="7043"/>
            <end position="7078"/>
        </location>
    </feature>
    <feature type="domain" description="GAR" evidence="10">
        <location>
            <begin position="7083"/>
            <end position="7155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="152"/>
            <end position="179"/>
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    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="196"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1650"/>
            <end position="1715"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1835"/>
            <end position="2058"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="4198"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="6845"/>
            <end position="6947"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="7183"/>
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        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
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    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
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            <dbReference type="PROSITE" id="PS51460"/>
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        <source>
            <dbReference type="EnsemblMetazoa" id="ACON029051-PC"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="7448" mass="823686" checksum="D1738361F792AEBE" modified="2020-08-12" version="1">[encoded data, 7448 characters]</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WCU5</accession>
    <name>A0A6E8WCU5_ANOCL</name>
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        <recommendedName>
            <fullName evidence="9">DH domain-containing protein</fullName>
        </recommendedName>
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        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
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            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
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            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="7">Ngousso</strain>
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        <subcellularLocation>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="666" mass="73562" checksum="D35CB7A59C2D38E5" modified="2020-08-12" version="1">MSNQGLIQPAKSPLPNARRSSLGGWGGLISSNQRPSTLSYAMSCAQCLQSWFQLACCQQPDCCTPDSSGGGQWRRQHHQAVGYDGANEEDRYQENENGALVLHPPSTGMTRKKWLPTQFRKLSHGKSDKLPPVEKTLISKKGSDKKVKPNQQEKPPEEQESSAAASAAQLEPEDEEATAIELPPPMKPIQDTASALSGASVVPSSAEDAAAGSTNKITKAMSLKSLEEPVGAQPPADLAEIEQFVKAKVESLKYLDNDGGGGSSSSSAAAALAMGLMVGDGSSSSGTMALNGGHTIAKPAISPQFSENEAADDTLTEEQCIEDILRKRNYALRELLTTEEAYVNDLSQIVNGYIAEIRNPASTVLIPDDLKGGKERMVFGNIEAIYEWHRDHFLKALQKCLQNPYELGPLIKRSERKLHMYVVYCQNKPVSEHIVQEHMSYFDELRLKLKYKLCLGDMLIKPVQRIMKYELLLKDILKHTLRAGLTEEVPGLKEAMHIMQVVPKAANDMMDVGRLQKFEGKITTQGKLLLHGPLYCVEGASSSDKNSYNSQKPKELHVFLFEQNIIFAEIVGKKTQFTSPNYIYKAHIQVNKMTLQDLSDQTNGNRFSLCSIDPQRSSLSYICTAPTPELHSEWLNTIHEILKTQNDFLKALVSPIAYQKELTKDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WCW2</accession>
    <name>A0A6E8WCW2_ANOCL</name>
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            <fullName evidence="2">cGMP-dependent protein kinase</fullName>
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        </recommendedName>
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    <organism evidence="18 19">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.12"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cGMP subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.12" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8WCW2"/>
    <dbReference type="EnsemblMetazoa" id="ACON029459-RB">
        <property type="protein sequence ID" value="ACON029459-PB"/>
        <property type="gene ID" value="ACON029459"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001525"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON029459"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_036639"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030553">
        <property type="term" value="F:cGMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004692">
        <property type="term" value="F:cGMP-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
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        <property type="entry name" value="AGC-kinase_C"/>
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        <property type="entry name" value="Prot_kinase_dom"/>
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        <property type="entry name" value="Protein_kinase_ATP_BS"/>
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        <property type="entry name" value="RmlC-like_jellyroll"/>
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        <property type="entry name" value="Ser/Thr_kinase_AS"/>
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        <property type="entry name" value="CYCLIC NUCLEOTIDE-DEPENDENT PROTEIN KINASE"/>
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    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
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    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
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            <begin position="1"/>
            <end position="79"/>
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        <location>
            <position position="251"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="532" mass="61009" checksum="640DB25F9C013BDC" modified="2020-08-12" version="1">MIRKKEGRVEVSREGKYLSTLSGAKVLGELAILYHCQRTATITAATDCKLWAIERQCFQTIMMRTGLIRQAEYSDFLKSVPIFKNLPEDTLCKISDVLEECYYQKGDYIIRQGARGDTFFIISKGQVRVTIRQPDTQEEKFIRTLGKGDFFGEKALQGDDLRTANIICDSPEGVTCLVIDRDTFNQLISNLDEIRNRYNDEGVSQRKKIWEEFREVKLSDLRVISTLGVGGFGRVELVQLAQDKSRSFALKQMKKAQIVETRQQQHIMSEKEIMSEANSDFIVKLYKTFKDRKYLYMLMESCLGGELWTILRDRGHFDDGTTRFYTACVVEAFDYLHSRNIIYRDLKPENLLLDVSGYVKLVDFGFAKKLQSGRKTWTFCGTPEYVAPEVILNRGHDISADYWSLGVLMFELLTGTPPFTGADPMRTYNIILKGIDAIEFPRNITRNASALIKKLCRDNPTERLGYQRGGISEIQKHKWFDGFYWEGLRNRSLPPPILPKVQSVVDTANFDDYPADPDGPPPDDLSGWDDDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WD62</accession>
    <name>A0A6E8WD62_ANOCL</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8WD62"/>
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        <property type="protein sequence ID" value="ACON029563-PA"/>
        <property type="gene ID" value="ACON029563"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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            <begin position="32"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON029563-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="126" mass="14650" checksum="96F6D618D841CFBF" modified="2020-08-12" version="1">MFVAVSLLFARVACRNPKGSLMTRKRPVLDIHRNLGNPPNKMPTQEQTTRPSVCYLPTPDRSFSDRKQYRCSKRMATFMGIGGTNARNTNSREKPIYFDQFYDISGCNRKFRGSTSPREDFRKRFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WD81</accession>
    <name>A0A6E8WD81_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="9">BHLH domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8WD81"/>
    <dbReference type="EnsemblMetazoa" id="ACON029238-RB">
        <property type="protein sequence ID" value="ACON029238-PB"/>
        <property type="gene ID" value="ACON029238"/>
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    <dbReference type="VEuPathDB" id="VectorBase:ACON029238"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042229"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11405">
        <property type="entry name" value="bHLHzip_MLXIP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000094">
        <property type="entry name" value="Blast:Carbohydrate-responsive element-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052207">
        <property type="entry name" value="Max-like/E-box_TFs"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15741">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX ZIP TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15741:SF37">
        <property type="entry name" value="LD38259P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="8">
        <location>
            <begin position="659"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="179"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="405"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="616"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="857"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="710"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="185"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="411"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="497"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="616"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="640"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="654"/>
            <end position="668"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON029238-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="885" mass="94404" checksum="3F57D9EC62279D79" modified="2020-08-12" version="1">MKRKTLVCQFASPLDVDVHNTPQAILLEGKYWKRKCNVIKAEYKKWRRFNVNRALGATNIVDTTSELDFLEWSPISNDIFMITDNMPTDTLFSTLAQYPFPDSREIARGAGRADFIQPSLGPLQPNLDDLMDLDLDFLNFPRLAPVPEEASDDLLKAIEYNYTLGSNQLTNAGNSIQEAPETLGVSGNHQQTSQAHQATGCIQQQPPAALQYSAKLYAQSLQPPSGGSSASISSAASKFTRAYPKHNYDPKYPPTPHQTMAYNMVSQPHMGQTAGSNAVLGATASGGPYGSNASSGFQNVISSAATAPSGMLQMGSGGIPPSLTPSSMQLHYNHSNAATNPVNLSNSPAGTNIVGGAGGGGSSNVRAPTQMGTAPTAGQSNVAAGSVLLMQNLNSKLLTHSQSLPVPALPQTKQVPAQPRSTSMPTGPSNYNQMLAASQHSPPGSLHASQSGSSYKSYHGHQPQPYKIPSQTVGAGGSYASGVRAMRHSSPPHGTVLQPTSGSSGASLPINATFPLPPKEGDNFAMPRYHQQQQSSQKANAKLRTRSNSMIMKQQTGGVLNSIPGMGMVGSGSAAGGMAGSSQHMSPTLHATSSEPMLNVTNSALLAQLLTTNSNGMSLSQQSLSPESAHDTDGPLSPTGPGGSTSGGAGSKFPRSDSQRRTGHIHAEQKRRYNIKNGFDTLHSLIPQLQQNPNAKLSKAAMLQKGADYIKQLRSERSSATEQMDSLRREIEQLNNSLNNLHTALPASGAPVSRQRTGRVKELYHHYVRQRTLDNWKFWIFGLIFEPLLNSYNQTVSVASMDEMYRTSQLWVDQHCSLVELRPAVSNQLRQLSTTTDVLSDPPSSLQEEVLKAISNSSSNCLAPRGSGKGGSRGGSSTETSPHRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WD83</accession>
    <name>A0A6E8WD83_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="24">Neural-cadherin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="22">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Cadherins are calcium-dependent cell adhesion proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 16">Cell membrane</location>
            <topology evidence="1 16">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_049465819.1">
        <property type="nucleotide sequence ID" value="XM_049609862.1"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON029589-RA">
        <property type="protein sequence ID" value="ACON029589-PA"/>
        <property type="gene ID" value="ACON029589"/>
    </dbReference>
    <dbReference type="GeneID" id="120959188"/>
    <dbReference type="CTD" id="35070"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007424"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON029589"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034794"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016342">
        <property type="term" value="C:catenin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005911">
        <property type="term" value="C:cell-cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008013">
        <property type="term" value="F:beta-catenin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045296">
        <property type="term" value="F:cadherin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048846">
        <property type="term" value="P:axon extension involved in axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044331">
        <property type="term" value="P:cell-cell adhesion mediated by cadherin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030855">
        <property type="term" value="P:epithelial cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016318">
        <property type="term" value="P:ommatidial rotation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007424">
        <property type="term" value="P:open tracheal system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045467">
        <property type="term" value="P:R7 cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045463">
        <property type="term" value="P:R8 cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="CDD" id="cd00053">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00110">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000182">
        <property type="entry name" value="Blast:Putative neural-cadherin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.900.10:FF:000001">
        <property type="entry name" value="Cadherin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000013">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000039">
        <property type="entry name" value="FAT atypical cadherin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000058">
        <property type="entry name" value="FAT atypical cadherin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000417">
        <property type="entry name" value="neural-cadherin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000040">
        <property type="entry name" value="neural-cadherin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000044">
        <property type="entry name" value="neural-cadherin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000112">
        <property type="entry name" value="neural-cadherin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000199">
        <property type="entry name" value="neural-cadherin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000209">
        <property type="entry name" value="neural-cadherin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000213">
        <property type="entry name" value="neural-cadherin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000312">
        <property type="entry name" value="neural-cadherin isoform X10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000184">
        <property type="entry name" value="neural-cadherin isoform X12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000109">
        <property type="entry name" value="neural-cadherin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000128">
        <property type="entry name" value="neural-cadherin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000222">
        <property type="entry name" value="neural-cadherin isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000220">
        <property type="entry name" value="neural-cadherin isoform X5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000192">
        <property type="entry name" value="neural-cadherin isoform X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000274">
        <property type="entry name" value="neural-cadherin isoform X9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.900.10">
        <property type="entry name" value="TCF3-CBD (Catenin binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039808">
        <property type="entry name" value="Cadherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000233">
        <property type="entry name" value="Cadherin_Y-type_LIR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027397">
        <property type="entry name" value="Catenin-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001791">
        <property type="entry name" value="Laminin_G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056370">
        <property type="entry name" value="Shg-like_Ig-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027:SF438">
        <property type="entry name" value="CADHERIN 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027">
        <property type="entry name" value="CADHERIN-23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01049">
        <property type="entry name" value="CADH_Y-type_LIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24811">
        <property type="entry name" value="Ig_Shg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02210">
        <property type="entry name" value="Laminin_G_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00282">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50025">
        <property type="entry name" value="LAM_G_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 14 16" id="KW-0106">Calcium</keyword>
    <keyword evidence="9 16" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12 15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 15" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="4 16 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="41"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="2922"/>
            <end position="2945"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="21">
        <location>
            <begin position="199"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="21">
        <location>
            <begin position="451"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="21">
        <location>
            <begin position="552"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="21">
        <location>
            <begin position="658"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="21">
        <location>
            <begin position="764"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="21">
        <location>
            <begin position="865"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="21">
        <location>
            <begin position="976"/>
            <end position="1084"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="21">
        <location>
            <begin position="1085"/>
            <end position="1189"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="21">
        <location>
            <begin position="1199"/>
            <end position="1305"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="21">
        <location>
            <begin position="1305"/>
            <end position="1421"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="21">
        <location>
            <begin position="1422"/>
            <end position="1521"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="21">
        <location>
            <begin position="1522"/>
            <end position="1637"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="21">
        <location>
            <begin position="1638"/>
            <end position="1749"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="21">
        <location>
            <begin position="1750"/>
            <end position="1868"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="21">
        <location>
            <begin position="1869"/>
            <end position="1973"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="21">
        <location>
            <begin position="1973"/>
            <end position="2092"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="20">
        <location>
            <begin position="2349"/>
            <end position="2385"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="19">
        <location>
            <begin position="2386"/>
            <end position="2592"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="20">
        <location>
            <begin position="2595"/>
            <end position="2635"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="19">
        <location>
            <begin position="2638"/>
            <end position="2829"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="20">
        <location>
            <begin position="2872"/>
            <end position="2910"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="2375"/>
            <end position="2384"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="2625"/>
            <end position="2634"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="2881"/>
            <end position="2898"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="2900"/>
            <end position="2909"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU003318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU004357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON029589-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="24">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="3101" mass="347433" checksum="E9217CA42E773C99" modified="2020-08-12" version="1">MCSLGAASRMRPVATATMAAARGDGGGAIRNRNSRAAAKRVSVYVLAIYVALVCSVNVAAVAGKHHHHHQGGQQEFKFDPTNSFISLVLPHDTYPGYSIQKFTANFTGPNAPFSSISSYSSKPLNYRLLETDYSKYFTVLEDGLVMTTADLSPLVNRPVKLVVLEETPNSTATHQLQLYVMNRKDMLHFPTMLTDVVGQLSENKDGGTRVRDIPLLQANSARGAKTVIYSIVGGNEGDAFALEDSSTGTIGTTIRIDDPRKHGVWLVSAKPLDREHREQYTLAIEATDTKRINKAEVHLPVHITDVNDNRPVFGKPDYRFTVTGMKATTPAGNETVTWERFTSVGRVEAKDADGDKVAYRLTSPSNTVVIVPQTGELLLAGEPDAPELFVDVEAHDLRTPSLKSEKPARVLVEFVAPDPLPVILQHSGHEYQHSHRRDKRRVTRAVRPTKRIEFTEADGDTEGRNVFQLEKETDRETFKIRDENPWVTVETNGAVRVKKKWDYEELGPEKTIDFWVIITNQGHNAGLKYTDNQRVIIHVKDVNDEPPYFINRPLPMQAVVQLNAPPNTPVFTLQARDPDTDHNIHYFIVRDRTGGRFEVDERSGVVRTRGTDLFQLDMEYVLYVKAEDQNGKVDDRRFQSTPEERLSIVGGKRAPQFYMPSYEAEIPENQKKDSDIISVKAKSFADREIRYTLKAQGQGAGTFNIGPTSGIVKLAKELDFEDLRQPHVYSLVVTATEDSGGFSTSVELTIRVTDVNDNAPKFELPDYQAHNVDEDIPLGTSILKVKAMDSDSGANAEIEYLVSDDHFAVDSNGIIVNNKQLDADNNNAYYEFVVTAKDKGEPAKTGTATVRVYTKNKNDEEPKFSQQVYTPNVDENAGPNTLVTTVVASDKDGDNVRFGFVGGGTSSGQFVIEEITGVIRLHSKAISLDRDKYELNVTAMDDGSCCVNGDATIHTSTAVVVVFITDVNDNKPVFKDCGTYYPKVEEGAPNGSPVIKVQATDEDKGVNGQVKYSIVQQPNQKGTKFTVDEETGEVSTNKVFDREGDDGKFVSVTVKATDQGEPSLEGVCSFTVEITDVNDNPPLFDRQKYVENVKQDASIGTNILRVSASDEDADNNGAIVYTLSAPHSPNDLEYFEIQPESGWIVLKKPLDRERYRLKVYASDRGDPPSTADVDVELDVVDRNNKPPIWDRTIYGPIHIRENVTVGTVVTSVKASSGIEGNPTVFYRLMPGSTAQTNKLHTFYLQQRPDNGDTWADIKVNHPLDFETIKEYNLTVRVENNGAQQLASEATVYIQLEDVNDEIPLFTEREQETVLEGEPIGTKVTQVNAIDKDGTFPNNQVYYYIVDSPRNEGKDFFEINLQSGEIFTKVVFDREKQGAYALEVEARDGAPSARPNSNQQPNSVTKFIRIGIADKNDNPPYFDKALYEAEVDENEDIQHTVLTVTAKDHDESSRIRYEITSGNIGGAFAVKNMTGAIYVAGALDYETRKRYELRLAASDNLKENYTTVVIHVKDVNDNPPVFERPTYRTQITEEDDRNLPKRVLQVTATDGDKDRPQNIVYFLTGQGIDPDNPANSKFDINRTTGEIFVLKPLDRDQPNGRPQWRFTVFAQDEGGEGLVGYADVQVNLKDINDNAPIFPQGVYFGNVTENGTAGMVVMTMTAVDYDDPNEGTNAKLIYSIEKNVIEEETGSPIFEIEADTGVIKTAVCCLDRERTPDYSIQVVAMDGGGLKGTGTASIRVKDINDMPPQFTKDEWFTEVDETDGTNLPEMPILTVTVHDEDETNKFQYKVIDNSGYGADKFTMVRNNDGTGSLKIVQPLDYEDPLQSNGFRFRIQVNDKGEDNDNDKYHVAYSWVVVKLRDINDNKPQFERPNIEVSVYENADVGKTLETFKATDPDQGGKSKVSYAIDRSSDRQRQFSINQEGTVTIQRNLDREVTPRHQVKILAIDDGIPPKTATATLTVIVQDINDNPPKFLKDYRPVLPEHVPPRKVVEILATDDDDRSKSNGPPFQFRLDPGADDIIRASFKVEQDQKGANGDGMAIVSSLRSFDREQQKEYLIPIVIKDHGNPAMTGTSTLTVVIGDVNDNKMQPGSKDIFVYNYLGQAPDTQIGRVYVYDLDDWDLPDKKFHWESQEHPRFKLDEDTGMITMRQGTRDGRYHLRFKVYDRKHTQADVPANVTVTVREIPHDAVINSGSIRIAGITDEDFIRVWNYRTQSLSRSKADRFKDKLADLLNIDRENVDVFSVQLRRKHPPLTDVRFSAHGSPYYKPVRLNGIVLMHREEIEKDVGVNITMVGIDECLYENQMCEGSCTNTLDISSLPYMVNANKTSLVGVRVDVLAECTCGARNFSKAESCRSSPCHNGGRCMETRYGLSCSCPTGYTGPRCQQTTRSFRGNGWAWYPPLAMCDDSHLSFEFITRKSDGLLLYNGPIVPPERDELLVSDFISVELERGFPRLLIDFGSGTLELRVKTKKSLDDGEWHRLDIFWDTENVRMVVDYCKSAEVSEMEDGSPPEFDDSSCQARGTIPPFNEYLNVNAPLQIGGFYREQFDPTHYRWNYMPMGKGFDGCIKNLVHNSKLYDLAHPGLSRNSVAGCPQTEEVCSQSEQTSRCWEHGNCVGSFTEARCQCRPGWTGPACNIPTIPTTFKQQSYVKYALSFEPDRFSTQIQLRFRTREQHGELFRVSDQHNREYGILEIKDARLRFRYNLNSLRTEERDIWLNAIAVDDGQWHVVRVNRHGSAATLELDGGEGRRYNETFTFDGHQWLLVDKQEGVYAGGKAEYTGVRTFEVYADYQKSCLDDIRLEGKHLPLPPAMNGTQWGQATMARNLERNCPSNKPCANVICPDPFECVDLWNEYECTCGEGRVMSPDGKGCMDRNECLDLPCLNGGICVNQEPRLRYRCDCPDGFWGENCELIQEGQTLKLSMGALAAILVCLLIILILVLVFVVYNRRRESHIKYPGPDDDVRENIINYDDEGGGEDDMTAYDITPLQIPIGPMPELTQCKMPPLMYPVMTMGPGHEPNVGMFIEEHKKRADSDPNAPPFDDLRNYAYEGGGSTAGSLSSLQSGTDDEVQEFDYLDAWGPRFDKLANMYGDHHPTELDDLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9I3BD58</accession>
    <name>A0A9I3BD58_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Nicotinamide-nucleotide adenylyltransferase</fullName>
            <ecNumber evidence="11">2.7.7.1</ecNumber>
            <ecNumber evidence="11">2.7.7.18</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2023-03" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>beta-nicotinamide D-ribonucleotide + ATP + H(+) = diphosphate + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:21360"/>
            <dbReference type="ChEBI" id="CHEBI:14649"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="EC" id="2.7.7.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>nicotinate beta-D-ribonucleotide + ATP + H(+) = deamido-NAD(+) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22860"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57502"/>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
            <dbReference type="EC" id="2.7.7.18"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from nicotinamide D-ribonucleotide: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; NAD(+) biosynthesis; deamido-NAD(+) from nicotinate D-ribonucleotide: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the eukaryotic NMN adenylyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.1" evidence="11"/>
    <dbReference type="EC" id="2.7.7.18" evidence="11"/>
    <dbReference type="RefSeq" id="XP_040233938.1">
        <property type="nucleotide sequence ID" value="XM_040378004.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9I3BD58"/>
    <dbReference type="EnsemblMetazoa" id="ACON030692-RB">
        <property type="protein sequence ID" value="ACON030692-PB"/>
        <property type="gene ID" value="ACON030692"/>
    </dbReference>
    <dbReference type="GeneID" id="120956503"/>
    <dbReference type="CTD" id="42987"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040521"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000309">
        <property type="term" value="F:nicotinamide-nucleotide adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004515">
        <property type="term" value="F:nicotinate-nucleotide adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09286">
        <property type="entry name" value="NMNAT_Eukarya"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000191">
        <property type="entry name" value="Nicotinamide-nucleotide adenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051182">
        <property type="entry name" value="Euk_NMN_adenylyltrnsfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005248">
        <property type="entry name" value="NadD/NMNAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045094">
        <property type="entry name" value="NMNAT_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00482">
        <property type="entry name" value="nicotinate (nicotinamide) nucleotide adenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12039">
        <property type="entry name" value="NICOTINAMIDE MONONUCLEOTIDE ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12039:SF0">
        <property type="entry name" value="NICOTINAMIDE-NUCLEOTIDE ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 11" id="KW-0520">NAD</keyword>
    <keyword evidence="7 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 11" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="4 11" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cytidyltransferase-like" evidence="12">
        <location>
            <begin position="12"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON030692-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="249" mass="28211" checksum="487790B7D5BE0E1E" modified="2023-06-28" version="1">MRMTSSTKIMLIACGSFSPPTPMHFRMFEIARDHIQQMGLGQVVGGIVSPVHDSYAKKGLVSATHRCAMIKIGLKTSDWIHLSDWETQQEEWTRTRQVLQYHQNYINSYLKDTNGTINNQHIPAWIPEGIKRTAGQVQLKLLCGADLLESFATPGLWKDEDLEAILGYHGIVVISRAGSNPEQFIFNSDLLSRYRRNITIVTNWVTNDVSSTLIRKLLSRGLSVKYLLDDHVAEYIRKFGLFGTNNETS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9I3BD71</accession>
    <name>A0A9I3BD71_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Calponin-homology (CH) domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
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        <scope>IDENTIFICATION</scope>
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</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A182KZK3</accession>
    <name>A0A182KZK3_ANOCL</name>
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        <recommendedName>
            <fullName evidence="4">Sorting nexin</fullName>
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        <name type="scientific">Anopheles coluzzii</name>
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            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
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            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
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        <text evidence="4">Involved in several stages of intracellular trafficking.</text>
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        <property type="gene ID" value="ACOM029629"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR45850">
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    </dbReference>
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        <property type="entry name" value="Vps5"/>
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    </dbReference>
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    </dbReference>
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        <location>
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            <end position="190"/>
        </location>
    </feature>
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            <begin position="9"/>
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    </feature>
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        <source>
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        </source>
    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="PIRNR" id="PIRNR036924"/>
        </source>
    </evidence>
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        </source>
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        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004487-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="424" mass="48013" checksum="65B32A08C1F884F3" modified="2016-09-07" version="1">MMDGIEENGSPQQATSPSVTVPETIGSRQGSVEGGSTTGTTAALPENSLLVDISDALSEKERVKFTVHTRTNLPGFDKSDFLVVRQHEEFVWLHDRFEENEEYAGYIIPPCPPRPDFDASREKLQRLGEGEGNMTKEEFKKMKLELEAEYLATFKKTVAMHEVFLTRLASHPVFREDSHLKVFLVYDQDLCAKMKKKIDIFGGLVKSIGKTTDEIYLGATVKDVNDFFEHELQFLGEYHSHLKEAAIRTEKMTNKHKDVADSHVRISSQLLALSTAEHGSMEKFLAKTSDIFEKIRNMEGRVASDQDLKLGDTLRYYQRDSNAAKALLIRRLRCLAAYEAANRNLEKARAKNKDVHAAESAQTQACEKFESMSARGKEELVSFRLRRVAAFKKSLTDLAELEIKHAKGQYEFLRQSLLALQELV</sequence>
</entry>
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        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
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            <taxon>Metazoa</taxon>
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            <taxon>Hexapoda</taxon>
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            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8V0I5"/>
    <dbReference type="EnsemblMetazoa" id="ACON001590-RB">
        <property type="protein sequence ID" value="ACON001590-PB"/>
        <property type="gene ID" value="ACON001590"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009089"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001590"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039062"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:fibroblast growth factor receptor binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:transmembrane receptor protein tyrosine kinase adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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            <end position="338"/>
        </location>
    </feature>
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            <begin position="154"/>
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        </location>
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        </location>
    </feature>
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        </source>
    </evidence>
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    </evidence>
    <sequence length="996" mass="108338" checksum="AB659C133DC2B45E" modified="2020-08-12" version="1">MDVLFVTARHSEHGMLWVNHLKVCFDKITKQRGRLPFKFLHIKLDEDVVQQTPHIIQQCESTKLQIVVVCPALLGLSPSLLHTKLNALLTPNRVLGMLLDVSECAVNEFAKTSLPGYSRWRTCSVREKDTAFVSELLGIATDILGKALRQQTVLQRTPSGTEPKTPTSPTGPTGTGTAGHLPGAAASGTGSGHRSHDSFVLFPRKVKVGQSKILLILHEPLTKEDWIKIKVEKADQTIEITNIKRRNPYTIQFSVPEVCMEISMMIKIRLEKNSIDLGSRPLKCESQMRELEQILSSKDAPMELICQSVGLSTNDRDKLDLLLLKAFLRNVPPNYNLLNYGSGGGGAEMGCHRDIYPEEYPTLLHFAARWGLERLCMQLIESPGGEIACEMRNINGRTPSDIAELAGHYKIASALKNFSQMNELTTMYHYFKGVSGASSDQVMIQPKQQQHGTGLPIRTQNKTDAEKILSHVHALKQAEGYIDMSCANGSDQVDGSSGASAINIHYLNDRPAGSELTPPGPPDEKDVCGENEDNFDVVRDTDRRCNNGSLRSVESAERAAKYDIFSNECSDLLENCEAGSTASAECVTNGDYMLQPSNAPFTPPTERTPTPPPLPATAEPMGDYMIQPSNIPVHPYCNVPEVVPETSRPPSSAEGRTVDDELLEIISDFKNNVFSIQEVEQLVTLWKNRNDVQKSFREKQDQLARMREHYEQIQRELKDKLKRPTPFERMRSFFSRTKASPAGGKAAAAKQRAGNGSAGGASDPPGADVCDEIKFSMAGGGGSNGGYAVVRPQSLSLSMNSMGSDNSEQSSGGRLSSGGGPPASTPTDGAPSEGRRLSHSKSWPKGTENENYMVPPSPRPISEELHLVRGVRLGSGTLVGALNRCSNGSCSSTASSHSNHSSHSSSSAGSWAGMGKQESHYVMYPSNVPVFAGGAEYMNVPAAAAVTHHTKEDERMTARPKLGRTPSDTGGPTPHLVTFQSIMECNESADSVDSVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V2J9</accession>
    <name>A0A6E8V2J9_ANOCL</name>
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        <recommendedName>
            <fullName evidence="19">Neuroglian</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_040237545.1">
        <property type="nucleotide sequence ID" value="XM_040381611.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8V2J9"/>
    <dbReference type="EnsemblMetazoa" id="ACON000720-RA">
        <property type="protein sequence ID" value="ACON000720-PA"/>
        <property type="gene ID" value="ACON000720"/>
    </dbReference>
    <dbReference type="GeneID" id="120958624"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001350"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000720"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039072"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
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            <strain>Mali-NIH</strain>
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        <citation type="submission" date="2020-05" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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            <begin position="1559"/>
            <end position="1578"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="42"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="127"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="162"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="172"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="197"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="257"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="426"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="472"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="491"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="795"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="828"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="845"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1562"/>
            <end position="1578"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002737-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1578" mass="176880" checksum="BDBB81087806E20A" modified="2020-08-12" version="1">MTGRRERRSRSKKNQPEEPVQVEVTPAEPTMLDLLKIPGRGDSPRDFRSLHREKSPFEIGERPEKQVASQKAIIDKALVMDISGGVTNVDEYISLFFTDSCSLDPASCGDISTVKVVVVEEEMVEPEPPKPAEEHSKTEAEAAAELARIQEEEEAGKSAKEPTPPPPPPPEPEPEPEPVEEEEEEEEEEEEPEPEPPRAPTPPPPKEPTPEPEPIKPPPEPEYDSDVELIPVKEEFDPSVWKSIDSLELQLQQQAEAEAKPKAKEKEKSREASRRESTTQEMTERERDLEWQRQRQMMRPPLVISHLKARAAPKGSTVKLTCTISGPGITVRWLKNGDPIEKSTKHTFKVSEGLLSLEIKDIDNADAGEYCCMIKNKNGETSTSTSLTVYENYETKPTPPTFISIKDDVSLEDKQQQQQAPAVVASEGQAAEAAATASTPEQPAAVAEATATAGDAPAAEQPVSGDAALPPSGAPQPPPAQPQEQPPPAPAAVEAPAAPTTEEQPAGEEGEAPAAPPPEVGGHAKHRRTAPEKSPSPPPIGGDMARYYHKKILHQRDKLQWSVHLTNRALVAGHRLKLMCCATGFDPTLEWFKDGQPVEYDDHIVDMNDMHRGAYGCLWVNDVTVKDAGEYKCKAKNATEEIETVCRLTVVEPVNTVQTFAPTFVRMVRENYDPYSNILTVEFVIRANPTPTLTWYKGIIKLGVYPDSHMRISQHFDETAEHTIASLVFYDPSYTDNGEYICIATNTVGEATCKHVVDFCSKEQYFEWLAKKRPGWFKAESYIAVEPPPTFGDGEAGDEEEDGGEEEEEEAEGGEEGEGPPKVKRAPKLPDVKELPEEETKAEAEAQAPAEPRSVQIVEPEPVAPAKEPEPEPHYKRRKSRTQVALEEFERRKKFDFVSHMANVRVEPGKTLRLIAYVKCPEEVSSYWKRDGRVLGNGLRLTHTTMRCGTCVLEIEKCKYRDAGSYTCVAKCPTYGEIEQTCTVSVVEKTEIKGEAPVFTRPMQEKYDPIQDELTLECCVRGDPEPETVWIVQGVFMRHNTGGRLYFRKYPDGRQLLKIFQPSKEDSGRYVCRAKNSVDKTDMSYWLNYKNSDEDVLKVFEDELHKKSEKPILSRHLRAKDCDYNPEDEALIKWSETRSQHDKEYDYRYKLRFITQLQDKTVPEGSNLKFTCYVDGKFPLFMWYKDDIPLVQGRKYRQKTRRDGKVTLEIVNVTTDDAGTYKLEARNYAGSIETKSAVTVYENPYTKFVPPIFASNILETYSLHSNEIVLECRVRGTPRPNIAWIKDGEYIIPGDKYEQYDHADGTCKLIVTSPGEEDSGTYTCEAESGGCSDAISHNVQFVSKEKVLMERTHSVYHRNPNLPHFYQGLADYSIPSGGNICLVVEVQGNCEVQWFRDRYPVTGKPPKVRTYSDGTGLFALCITAATMDASGRYVCRATNAFGKAESSSNVDIINPNAIKGAKPPIFMSRPQPEIKIRQGDTLSMAFKIIGDPKPKIQWMKGTKDLTNSPRTVKEVHADYIRFSIKEAVVADEGAYFIVARNRHGIDRSFTKVSIKPPRGYKKHLEDDLDRDAPDKGRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V4N9</accession>
    <name>A0A6E8V4N9_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="17">PlsC domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">Ngousso</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="13">Phospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040218716.2">
        <property type="nucleotide sequence ID" value="XM_040362782.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8V4N9"/>
    <dbReference type="EnsemblMetazoa" id="ACON002084-RB">
        <property type="protein sequence ID" value="ACON002084-PB"/>
        <property type="gene ID" value="ACON002084"/>
    </dbReference>
    <dbReference type="GeneID" id="120947454"/>
    <dbReference type="CTD" id="137964"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012580"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002084"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_029825"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004366">
        <property type="term" value="F:glycerol-3-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019432">
        <property type="term" value="P:triglyceride biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07991">
        <property type="entry name" value="LPLAT_LPCAT1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045252">
        <property type="entry name" value="LPCAT1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23063:SF2">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE 4, ISOFORM D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23063">
        <property type="entry name" value="PHOSPHOLIPID ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="235"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="259"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="446"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="16">
        <location>
            <begin position="320"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="68"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="145"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="97"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="116"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="172"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00025707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002084-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="527" mass="58817" checksum="0C193ECF8AE2D091" modified="2020-08-12" version="1">MGGLQAAWSIMSMVTLTPFFAFLFSIVFMASIGKSFGVRRLYVNLLVKIFEFGRQNIESVRKQQFANITQSDPEDEDAPTGDNPSPDTADTGDGTGSAKTNGTLPNGGSHRYMNGRDTSSHIANGGNTVISRAESLILSPEMIDDTRSKSAEPQEEGAGGGTRDQGEEGDEETKSDGSEGGGEHEAAGFKLSNCLDYVKSGMEAIIEDQVTSRFLAEELKNWNLLTRTNRQYEFISWRLTVIWMIGFLIRYFILMPMRVLICFIGVIWLTLCTAVVGCVPEGSIKRALVKNVLIQCFGFLSSALSSVVNYHNIQNRPLNGICVANHTSPIDVLMLMCDNCYSLIGQRHGGFLGVLQRALARASPHIWFERAEAKDRILVAKRLKEHVTDPKNPPILIFPEGTCINNTSVMQFKKGSFEVGGVIYPVAIKYDPRFGDAFWNSSRYSMMQYLFLMMTSWAIVCDVWYLPPMERQEGESAIDFANRVKRVIADQGGLVDLVWDGQLKRSKPKKEWKEKQQEKFSKLLKGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V504</accession>
    <name>A0A6E8V504_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Troponin T</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the troponin T family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040222823.1">
        <property type="nucleotide sequence ID" value="XM_040366889.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_049462967.1">
        <property type="nucleotide sequence ID" value="XM_049607010.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8V504"/>
    <dbReference type="EnsemblMetazoa" id="ACON002350-RD">
        <property type="protein sequence ID" value="ACON002350-PD"/>
        <property type="gene ID" value="ACON002350"/>
    </dbReference>
    <dbReference type="GeneID" id="120949532"/>
    <dbReference type="CTD" id="136032042"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011848"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002350"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041535"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005861">
        <property type="term" value="C:troponin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005523">
        <property type="term" value="F:tropomyosin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006936">
        <property type="term" value="P:muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006937">
        <property type="term" value="P:regulation of muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045214">
        <property type="term" value="P:sarcomere organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
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        </source>
    </evidence>
    <sequence length="1934" mass="204384" checksum="688CC8C156BFEE57" modified="2020-08-12" version="1">MANSSETGTIVKDPPTAHTGPDSGPPSASEITIIMRTVVSSPGSDRTASAKNESALKRNETAALDTALHHLPGNRIEVTTSVRTVFECPAAAAADLGTAGSSGAATAISSLLQPVLSKNFIALLKSANDSSFENVLEFKSLLGAPATPAGRSCPSCEHAKHALSSDSDTTANDLDASVQKKVKTEEQHAAKVHVCEDDRGGFAQRTTSTPIKIRLVSGGDDAKAEAQTRYDAEDEEAENYRLNLLTPPADASFIDSDFESMEQDQYDTCSSKSSPRPDHRQPDRLLFGEPTSHPRDSVVSMEVAALSTVHSDRGPSTGDINCPLTKSSARKASSSTKAFFEEALRTPLAIRKHFQRRRNSELPKQPSHGNLLNFFSAKKKSNSLEGGLKSPRAKRSLTTTTTTTTTTSENSSENGSRCTSPALTEDNLRRLSVSFVRRESDSLNRTLVWGENYDYSYECEPSHSDVEEAEEPEDERNNDDENDDANHSPLVGTIGNGRTTERRVSAKLVHFEISPDCSYELQPPILPSFRIDPPRASSISTCSGSTTTGLAGLIIGAAAFATATTTASFLKRSASEPAHCNKLRSHSCTPLTSDIGESDEEGDDTLEGEEEPRRRNVRPADEAEDVPMMMCKALVPQRPFTSVHQASVRSLDKIGGKTINHSASPFRRWGQSSLRSRSTEHRTPTNGSARRPSSLAASESDVYTKSIDDDYSSTKSGYNPYTGGGSAGGGVDTLSAESATIAAGPMGTAGTGGSGGTTTGPTGVASWGTSFEKLLEDAAGLHTFSEFLKKEFSAENIYFWTACERYRQLTEREERAREAQAIFARHLESGCSEPVNVDSIARNIALENLPQAEPTLFAAAQKQIFNLMKFDSYQRFIKSDLYRVCQEAEGKGQGLPYPGEQLDPMLRTSATMLTASGTGTAASITKLKKSLSNAEDRRRKSLLPWHRKTRCKSKDRGDSDTSKKDSKEKGGGSGGGGGGEKASGTTATGSSSNTLKLLSTNSTSDIHSSRSSLASFDAAIGGKSCDPDDSRNTLCRVILSNGATTVVQTRSNETIKELVERLLEKRGIVYNAYEAFLAGSTKPLDLDGPSVSLAGKEVNIDQRVVFKLNLPNRKMISVKSKAAKPLADVLRPILHKYNYELDEMKVVVHSTVDVCLDMTQPVTTVDGLCLYIRSANETPNVHPELGRPLAGARPVINTATHQQIVHHFQQQQQQQLLQLQNGAQASAAGQQQHTFAVPSALPPSYAVATNSKSQQLLGNGTAASATCNNNTVNQTQSRAKEQSEQQSKDDQQQQQQAGVGAGTRGQQELNSLDEITNKVFDELRNGKTAFGGVVTAVGKGPGTVDVDCASDTSSTRRDRFRRRGSNAAPSESGRGAKSKKCSTGGSEDGGESVNNLGIKKPIIAKLKAGVKLQIPTRSQNDELLEGLKRAQRSRLEDQRGTEINFELPDFLKDKENFSSSSAAVSSSAGSIGASAAPSSAAGPSKLTRSKPARRSEATTPTAEFVGGSLQQINKPQPAPRLSITGQQGRQNLSGPGSPVHAAVSDSHLNLSTQCNASPLPQDDPFHVHGGGSGTNGGSQSEISYADTTLVFTHARPYNGQALVTATPRTIPSSSASQHYSGDHSSDSSSTADGPDLPNLACCEHHPHEMSPDSSSPPTHANHLHPLHYHPAGAGASGANGAAGATPNGNTSTNNPYPLAVHYPSHHHHHPHLHPHHHPHGQHYHHHHHHHTHNGGGGYQTATSQPVANGSNAPGQDGQPNATSGGAPDSQKGPPPLPPKPKILPIKPSNWGHPVAASYAPGPPGSAGAPGGGTSELLGGLSTTTLSTNGSMGGSVNGTGATVGGLSPSSSTVSSHHPNSNSNGVGVGTVVGSSNGIVSSTTHNDLSRGGREGNRQQRASSGNDGGSSNGSASAISVAVAQARNVYFDQGNSSFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V8Y3</accession>
    <name>A0A6E8V8Y3_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_040230078.1">
        <property type="nucleotide sequence ID" value="XM_040374144.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8V8Y3"/>
    <dbReference type="EnsemblMetazoa" id="ACON000696-RC">
        <property type="protein sequence ID" value="ACON000696-PC"/>
        <property type="gene ID" value="ACON000696"/>
    </dbReference>
    <dbReference type="GeneID" id="120953827"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012822"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000696"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031578"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026209702" evidence="2">
        <location>
            <begin position="26"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="154"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="190"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="190"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000696-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="214" mass="22325" checksum="24366AB56C6C2EF1" modified="2020-08-12" version="1">MKPFHSWSSAVTILTLALCAGVALAAPNVRVKRGDEVEVIADAESAVNPLASQDAVAIGDDLDRDKRKVPDPKYETKNAILGFVFGKINSFIDAKTRFIDKLDHANIEKNKQHHIEPPKPVPDFQSLISSVITPKVQFITSKIGSLSGSFLGGSSGGHGSDDDHHGNGGGGGAPQLGNIVSSLLKLSGPILSGSSGSQHGGSNTVSLGDHDDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V8Y4</accession>
    <name>A0A6E8V8Y4_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="3">MOSC domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_040226036.1">
        <property type="nucleotide sequence ID" value="XM_040370102.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8V8Y4"/>
    <dbReference type="EnsemblMetazoa" id="ACON000699-RB">
        <property type="protein sequence ID" value="ACON000699-PB"/>
        <property type="gene ID" value="ACON000699"/>
    </dbReference>
    <dbReference type="GeneID" id="120951384"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_003446"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000699"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034275"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030151">
        <property type="term" value="F:molybdenum ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005302">
        <property type="entry name" value="MoCF_Sase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005303">
        <property type="entry name" value="MOCOS_middle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011037">
        <property type="entry name" value="Pyrv_Knase-like_insert_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14237:SF19">
        <property type="entry name" value="MITOCHONDRIAL AMIDOXIME REDUCING COMPONENT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14237">
        <property type="entry name" value="MOLYBDOPTERIN COFACTOR SULFURASE MOSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03473">
        <property type="entry name" value="MOSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03476">
        <property type="entry name" value="MOSC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141673">
        <property type="entry name" value="MOSC N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50800">
        <property type="entry name" value="PK beta-barrel domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51340">
        <property type="entry name" value="MOSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="MOSC" evidence="2">
        <location>
            <begin position="196"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000699-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="340" mass="37273" checksum="2A785F58C16B3F3E" modified="2020-08-12" version="1">MLSKLLNDGAGGKPTAGQLLAVAAGVGVATALGYGVYRLLQYRAAQQPPSEWHRVGEVSDLWIYPIKSCGAVRVRQFQCSPIGPQVGFLRDRIFMVVKSADGKFITGRSHPTLVLVQPAFDAQYERMTLSAPGMMDIGVDVKKLLESSAPGSAEVWDQPVTAVDCGEEVARWLSRFLLSEDFGLRLVYYPLDRPTRPVREKNRIHRLLTARDSGALHDATSYMLVSEGSVADVNARLDKPVPALQFRANILVKGPSAFEEDDWKWIRIGDTVYRNVKPCTRCLFTNVDPETGVSSPEQEPLTTLRKYRLKPGLGQSPVVGMQMGIRTLGAIGLGDAVYVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V994</accession>
    <name>A0A6E8V994_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="12 13">Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase</fullName>
            <ecNumber evidence="3 13">2.7.4.24</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Bifunctional inositol kinase that acts in concert with the IP6K kinases to synthesize the diphosphate group-containing inositol pyrophosphates diphosphoinositol pentakisphosphate, PP-InsP5, and bis-diphosphoinositol tetrakisphosphate, (PP)2-InsP4. PP-InsP5 and (PP)2-InsP4, also respectively called InsP7 and InsP8, may regulate a variety of cellular processes, including apoptosis, vesicle trafficking, cytoskeletal dynamics, and exocytosis. Phosphorylates inositol hexakisphosphate (InsP6) at position 1 to produce PP-InsP5 which is in turn phosphorylated by IP6Ks to produce (PP)2-InsP4. Alternatively, phosphorylates PP-InsP5 at position 1, produced by IP6Ks from InsP6, to produce (PP)2-InsP4.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>1D-myo-inositol hexakisphosphate + ATP = 1-diphospho-1D-myo-inositol 2,3,4,5,6-pentakisphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:37459"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58130"/>
            <dbReference type="ChEBI" id="CHEBI:74946"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.24"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:37460"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>5-diphospho-1D-myo-inositol 1,2,3,4,6-pentakisphosphate + ATP + H(+) = 1,5-bis(diphospho)-1D-myo-inositol 2,3,4,6-tetrakisphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:10276"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58628"/>
            <dbReference type="ChEBI" id="CHEBI:77983"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.24"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:10277"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Cytoplasm</location>
            <location evidence="1 13">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the histidine acid phosphatase family. VIP1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.24" evidence="3 13"/>
    <dbReference type="EnsemblMetazoa" id="ACON000926-RA">
        <property type="protein sequence ID" value="ACON000926-PA"/>
        <property type="gene ID" value="ACON000926"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_003396"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000926"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042928"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033857">
        <property type="term" value="F:5-diphosphoinositol pentakisphosphate 1-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000828">
        <property type="term" value="F:inositol hexakisphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006020">
        <property type="term" value="P:inositol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032958">
        <property type="term" value="P:inositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07061">
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        <property type="entry name" value="Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase"/>
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    </dbReference>
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        <property type="entry name" value="Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase"/>
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    </dbReference>
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        <property type="entry name" value="Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phosphoglycerate mutase-like"/>
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    </dbReference>
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        <property type="entry name" value="Acid_Pase_AS"/>
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        <property type="entry name" value="His_Pase_clade-2"/>
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        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040557">
        <property type="entry name" value="VIP1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12750">
        <property type="entry name" value="DIPHOSPHOINOSITOL PENTAKISPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12750:SF9">
        <property type="entry name" value="INOSITOL HEXAKISPHOSPHATE AND DIPHOSPHOINOSITOL-PENTAKISPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00328">
        <property type="entry name" value="His_Phos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PPIP5K2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00616">
        <property type="entry name" value="HIS_ACID_PHOSPHAT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="VIP1 N-terminal" evidence="15">
        <location>
            <begin position="67"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="968"/>
            <end position="1029"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1043"/>
            <end position="1062"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1083"/>
            <end position="1182"/>
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    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1313"/>
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            <begin position="1459"/>
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        <location>
            <begin position="1483"/>
            <end position="1577"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1607"/>
            <end position="1660"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1763"/>
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    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1806"/>
            <end position="1842"/>
        </location>
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        <location>
            <begin position="1854"/>
            <end position="2000"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="45"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1085"/>
            <end position="1102"/>
        </location>
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    <feature type="compositionally biased region" description="Basic residues" evidence="14">
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            <begin position="1103"/>
            <end position="1124"/>
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    </feature>
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            <begin position="1145"/>
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    </feature>
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        <location>
            <begin position="1157"/>
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        <location>
            <begin position="1313"/>
            <end position="1328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
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            <begin position="1504"/>
            <end position="1516"/>
        </location>
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            <begin position="1621"/>
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        <location>
            <begin position="1947"/>
            <end position="1977"/>
        </location>
    </feature>
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            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
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            <dbReference type="ARBA" id="ARBA00012893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
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            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022679"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
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            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00034629"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00055071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00071668"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU365032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF18086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000926-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="2000" mass="216376" checksum="39E8A435659DDC47" modified="2023-06-28" version="2">MSYTELESGYQGLKQASSSATGDGLGGGAGALQTPSGRPGFYVGDEGDLDPTDGLDSDDSSTSGKQVVVAVCAMSKKSQSKPMKEILTRLQEFEYIRMVVIGEEIILNEPVDRWPLCDCLISFHSKGFPLEKAIQYAQLRQPYVINNLHMQFDIQDRRRVYAILQQEGIEIPRYAVLDRDSPDPKQHELVESEDHVEVNGIVFNKPFVEKPVSAEDHNIYIYYPTSAGGGSQRLFRKIGSRSSVYSPESRVRKTGSFIYEDFMPTDGTDVKVYTVGPDYAHAEARKSPALDGKVERDSDGKEIRYPVILSNAEKLISRKVCLAFKQTVCGFDLLRANGKSFVCDVNGFSFVKNSNKYYDDCAKILGNMILRELAPQLHIPWSVPFQLDDPPIVPTTFGKMMELRCVTAVIRHGDRTPKQKMKVEVRHQKFFEIFEKYDGYRYGHIKLKRPKQLQEILDIARSLLAEIQTKAADSEIEEKQSKLEQLKSVLEMYGHFSGINRKVQMKYQPKGRPRGSSSDDGKQDCSTFPLFISQFASIALFLIDFVVFFAADAPKEPSLVLILKWGGELTPAGRIQAEELGRIFRCMYPGGQSRQPGVGEGPGAQGLGLLRLHSTFRHDLKIYASDEGRVQMTAAAFAKGLLALEGELTPILVQMVKSANTNGLLDNDCDSSKYQNMAKSRLHELMQIDREFTAEDRDAINPGNAISINLAMNFVKNPVQCCAQVHSLIRSLMAVVAVKRDDPKTRDAVLYHGETWELMGRRWGKIEKDFCTKNKNYDISKIPDIYDCIKYDLQHNQHTLQFDLAEELYISAKYLADIVIPQEYGLTMHEKLTIGQGICTPLLKKIRADLQRNIEELGGEESVNRLNPRYSHGVSSPGRHVRTRLYFTSESHVHSLLTVLRHGGLLNVLTDEQWRRAMDYVSMVSELNYMSQIVIMLYEDPMKDPSSEERFHVELHFSPGVNCCVQKNLPPGPGFRPHSRNDSVTSKNASGDEDTTSRIDEENDTEEESSFSNNSSLHHTPSKTLPRTDADFGNIVASAGSANVKERRVKKMKSSSPIPIGSCHTVSGHEAMDLAKRLSEELAVQQQHQLQQQQHQLQQQQHPYHHHHHQQQQKHLYQQHHHHLTGSFGCGGNAKDITRPLSPDSEPRARSFEHQQQHHHHGHQHHHHHGKMHHHRSKGGKGALTMNTLRAAAERCQYAKMESVKDLLEATDGCGSSSGDGLLGYPMTTTTASAADESAAAAAGPTDGGADTGLATSTTTLAGGGGGCPPCCCGTGGTVRYPGSSHGSGSLSSCCCCCCCCCSTPSSSAGGLAGAGTGAGPAGSGGTGSSAATVRRQRHSIAGQMSYFKMLGTFSKKMATSTNSLFSTAVISGSSSAPNLRDMIPSTASPSGFGGVPPIRPLETLHNALSLKQLDAFLERMTIGPLFKTPASSPPPKHPLISGGPIALKGSLSVVSHDSIGEGIGSGSGSMQPPSSIVAATTLGSGTKTAEERTAASRAGTRNAGAAVGSTTVAAGMPGPNGNSTPANGGAQQSWSDHSSSMTSSISALSSGGPSSPNYSEVYSRGCPSSDMSASITSSTDGSLAAIASGPGEQLVALPHLFSASAQISSPKHPGSVELNKGGEDPEAPRQLDEPPGATGLLLDDGTRRRGSAMDVSTGDLTPVSACSDWGDSNTNDATKGSITNYDLTTANTTEEEDEDATISTDTCLSVGEQQHHQQCCLMPCTGMDASFASSSSIKEGSGMVSVNSSAVGKKVSPFRGALPFDEPLNPADGGSDPHELDTSKSENRVVRGFRIQRQISLYENQKEEGKSVSQQQVHDWCGGGGSKSDLPASGRQQQQQPLSRLHMSFDELTHSDRNTTPAGRLAQSSITSSAAAAAPPPPPIPHTPGALVIREGFIEPPRLTRVTKSFHGKTNHQRFGVEEQQHGSESTPDGGYRRASDGPVATTGTARYSKTTLRPQHSSAAGVSSRFTTSIVQEPEQLRATEPDTEPSAASSKTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V997</accession>
    <name>A0A6E8V997_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Coronin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">F-actin regulator involved in anterograde Golgi to endosome transport: upon ubiquitination via 'Lys-33'-linked ubiquitin chains by the BCR(KLHL20) E3 ubiquitin ligase complex, interacts with EPS15 and localizes to the trans-Golgi network, where it promotes actin polymerization, thereby facilitating post-Golgi trafficking. May play a role in the maintenance of the Golgi apparatus morphology.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the WD repeat coronin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040224105.2">
        <property type="nucleotide sequence ID" value="XM_040368171.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8V997"/>
    <dbReference type="EnsemblMetazoa" id="ACON000930-RA">
        <property type="protein sequence ID" value="ACON000930-PA"/>
        <property type="gene ID" value="ACON000930"/>
    </dbReference>
    <dbReference type="GeneID" id="120950252"/>
    <dbReference type="CTD" id="31620"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011389"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000930"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035480"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000076">
        <property type="entry name" value="Coronin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000362">
        <property type="entry name" value="Coronin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015505">
        <property type="entry name" value="Coronin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015048">
        <property type="entry name" value="DUF1899"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10856">
        <property type="entry name" value="CORONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10856:SF20">
        <property type="entry name" value="CORONIN-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08953">
        <property type="entry name" value="DUF1899"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16300">
        <property type="entry name" value="WD40_4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01166">
        <property type="entry name" value="DUF1899"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01167">
        <property type="entry name" value="DUF1900"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101908">
        <property type="entry name" value="Putative isomerase YbhE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 8 9" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="DUF1899" evidence="11">
        <location>
            <begin position="4"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="75"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1899" evidence="11">
        <location>
            <begin position="653"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="777"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="820"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="430"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="541"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="621"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="493"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="573"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="630"/>
            <end position="646"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU280818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000930-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1119" mass="123111" checksum="F5FE0BFB383500F6" modified="2023-06-28" version="2">MAWRFKASKYKNAAPIVPKPEACIRDIFVGSYQTYGNNIAASAAFIAFNWEHAGSSLAVLPIDDCGRKSKTMPLLHAHSDTVTYLDFSPFHDGLLATGSQDCLVKVWHIPEKGLEQSLSTPECTFTTKQRRVETVGFHPTADCLLYATAVGWVSLWDLTTQQEAFSNNEHPEVIQSLSWKQDGRLCVTSCKDKMVRVLDPRASAPITLAAESHQSIKDSRVVWLGEQNRILSTGFDAARLRQVIIRDLRNFSVPEKTLELDCSTGILMPLYDPDTNMLFLSGKGDTTINYLEVTDKDPYLIEGIRHSGEQTKGACLVPKRALKVMEGEVNRIMQLTSNSVIPIMYQVPRKTYRDFHNDLYPDTNGYKSEMTATQWLNGANTPVPKISLDPAKREHGDAPIIPRLGPKPFCSTGSSGDFSFEKVFNVPAVPGAEPGSEKESAALPGPTVGDSSAPAEKNGNTLTVSPEPDEPKDDSPTEPGKPEPAEPMDQCDVETRTIPHAELRKPGEQEKIFERQASSESVDQFDATADDAKGQFERLSAQRTSLAERRRMFESRSQSVQDGEKASSPTPLRRRESLKVRGEQKSCADDTDLMPPPPATDFLRNRSDLSKENMPDIVKRSNSTAGVSTVASSNNASANNNNNNVASKRTSTVFGRVSKFRHLKGTPGHKSTHIENIRNLSRQIPGECDGFAANAARVAVPIAGAGGKIAVLELNDPGRLPDGVIPTLVNIQNIMDFQWDPFDASRLAVACDDGSVKLWRIPAGGLTEPTNEPEAVLVAHADKLYTLRFHPLAQNVLLTASVDMVMKVWDLGTMEEKCTLYGHTDQIFCFSWSPCGRYGATVSKDGRIRVYEPRQSSQPIGEGTGPVGTRGARVLWALEGDYLVVTGFDKVSERQIYVYKVTDLSAPMGMVGLDVSPAILMPYYDEDSSTLFATGKGDSTIYCFEITDEAPFICPLSHHRCTNLTQGLSFLAKNQCDVAAVEFARALRLTNGTVEPLSFTVPRLKVELFQDDLFPPTRVLWQPTLTAAEWFAGRDVAAARISMKPDGMDTLSSIQPTVTTTPSKKSDTVLMTQGIHKPFAKAPEHVQEQIKNAVSARMSVNKKLEQDSMEGVDEKEWEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VAF2</accession>
    <name>A0A6E8VAF2_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Deoxynucleoside kinase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DCK/DGK family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040230351.2">
        <property type="nucleotide sequence ID" value="XM_040374417.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040230352.2">
        <property type="nucleotide sequence ID" value="XM_040374418.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VAF2"/>
    <dbReference type="EnsemblMetazoa" id="ACON001585-RB">
        <property type="protein sequence ID" value="ACON001585-PB"/>
        <property type="gene ID" value="ACON001585"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON001585-RC">
        <property type="protein sequence ID" value="ACON001585-PC"/>
        <property type="gene ID" value="ACON001585"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON001585-RD">
        <property type="protein sequence ID" value="ACON001585-PD"/>
        <property type="gene ID" value="ACON001585"/>
    </dbReference>
    <dbReference type="GeneID" id="120953957"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001908"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001585"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035601"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019136">
        <property type="term" value="F:deoxynucleoside kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01673">
        <property type="entry name" value="dNK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001571">
        <property type="entry name" value="Deoxynucleoside kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002624">
        <property type="entry name" value="DCK/DGK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050566">
        <property type="entry name" value="Deoxyribonucleoside_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031314">
        <property type="entry name" value="DNK_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10513">
        <property type="entry name" value="DEOXYNUCLEOSIDE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10513:SF24">
        <property type="entry name" value="THYMIDINE KINASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01712">
        <property type="entry name" value="dNK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000705">
        <property type="entry name" value="DNK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Deoxynucleoside kinase" evidence="4">
        <location>
            <begin position="20"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="99"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="23"/>
            <end position="31"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="158"/>
            <end position="162"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000705-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000705-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001585-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="246" mass="28101" checksum="4C979D3EBA241F3A" modified="2020-08-12" version="1">MPPIASEKLGASGKKPFTVFVEGNIGSGKTTFLDHFQKFNDICLLTEPVEKWRNCGGVNLLDLMYKESHRWAMPFQTYVTLTMLDMHTCQTDKSVKLMERSLFSARNCFVESMLASGSLHQGMYNVLQEWYDFICCNIHIQADLIVYLQTSPEVVYERMKQRARSEESCVPLEYLKELHELHENWLIHGASPRPAPVLVLNADLDLNTIGAEYERSETSILKPILIENTNQHAILTSPAKRAKTDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VAK3</accession>
    <name>A0A6E8VAK3_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Synapse</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON001690-RB">
        <property type="protein sequence ID" value="ACON001690-PB"/>
        <property type="gene ID" value="ACON001690"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_008549"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001690"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037934"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048788">
        <property type="term" value="C:cytoskeleton of presynaptic active zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042734">
        <property type="term" value="C:presynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044325">
        <property type="term" value="F:transmembrane transporter binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048791">
        <property type="term" value="P:calcium ion-regulated exocytosis of neurotransmitter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050806">
        <property type="term" value="P:positive regulation of synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048167">
        <property type="term" value="P:regulation of synaptic plasticity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04031">
        <property type="entry name" value="C2A_RIM1alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04028">
        <property type="entry name" value="C2B_RIM1alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000003">
        <property type="entry name" value="Regulating synaptic membrane exocytosis protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000188">
        <property type="entry name" value="Uncharacterized protein, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000453">
        <property type="entry name" value="Uncharacterized protein, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010911">
        <property type="entry name" value="Rab_BD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039032">
        <property type="entry name" value="Rim-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054386">
        <property type="entry name" value="RIM_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12157:SF21">
        <property type="entry name" value="RAB3 INTERACTING MOLECULE, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12157">
        <property type="entry name" value="REGULATING SYNAPTIC MEMBRANE EXOCYTOSIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22601">
        <property type="entry name" value="RIM2a_ZnF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50916">
        <property type="entry name" value="RABBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0770">Synapse</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 7" id="KW-0863">Zinc-finger</keyword>
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            <begin position="4"/>
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    <sequence length="2735" mass="300092" checksum="E4F6A37E06A8CF41" modified="2020-08-12" version="1">MDDMPDLSHLTQEERAIIEGVMMRQKQEEERENEIMRRKQDEVATLVDSIRQKSEQQKKAGVELEATCHICLKTKFADGIGHICHYCNIRCCAKCGGKVTLRNNKVIWVCIVCRKKQELLSKTGQWMNKSTSPDGVIRRQEGDPRTLPQTMLDPHDPSDKRPKLERARSAAEKENNPMQRANSQLRRQYSQQDPPTRRLSQSDGSGMDMMMSPGHHQQQIGRMQQMGHGQVGGMYGGRPGAGQQGYGGGPYGQHSQLPQPPMHHGGGGGGGGPYGGQHSMTPQVHITHAGGGGYGHDDPSYYQSEIEDLMRTHPHLVHPRQQQHYAESLQSSSAHGSSGMGGGGGGVGSGGMHLPPEPTKSHKRPLGGPYLPQQRSFSSSDEDIRSTPEFEAGHQRQYFDNHTRLNANLTDYRATKLDVQQRNSYNRTNHNRYQSPSQAAPHSSHPYHYQPSQDPLGRDYHHQQPLPQVVVGDSTIAGYDSISHHHHQPHQPHQSLHHLSGTVAPSPVTIPQGLHGGSSGSSSSSQQHHPYQQQPHQQQCNSSSSGGSSHSTTGHHPVAQNSHARYGTSPAGTEQPDPYWDEPADSRRFTERRKKTVRFDGQDSDDWSRWESERQGSQDSATKDSGIDTSSTFTSSEDSNRGDGPKNPLSWQVVSSSDGGRPIGPAVQRRPFDGEDVLGLKVRGGQVLPSDPRAALIELESAHIRPENYDRRDKPSVLVTSPGSPDLHSVRNYSSSRYSNRLAPAGTVTQENSVGGRVQIKLGFEPSSLQLIVTILCANGLVPRGNGAARNPYVKICLLPDRSEKSKRRTKTLALTNDPRWGQTFVYEGLRRADLNNRLFEVTVWDYVRYGANDFLGEVIIDLSTHPLDDEAEWYILQPHQETLRDTVQPGNELDMILTPTDHLSPPSTTSRLSDSDTTSECDIDGLMTGRDGASVSSLGSSSSPPPEVDLQDRRSRRDMSPQGRKRAAGMVAKDYRTVSGVGQGFHNQMSAEVCQSSQRERHPSLTVYLCFLATGIVGVQAQRYDGVESAKPIGGTERQLSGRSAGLKRQLPQVPHMSRNAAIRERFIQDFEERSGGRFARHRGRQSHHQPTYRSTGMGGWERHYSGLSDSDLTTHSLESRIRPRHSLSPDKDFMGDFGDSDMESVVSVTSSAFSTQSERPRGSKGIRRAAAWRACFGASVYLEGQHGCGKTHQNRRLQHRSLPTGWATTSLCALPSESYPCGGQQVMADNSGILPVLPDVTQATIIPPKPEFELPPPACTVVGPSVVGNASTCSSFVSPLPVSIPIDSVLSEPIPISPAPPDKTISQMSDMAGSYGSVPVSHGGSMPEGYFNEQCITLQPERSTIRSISMNPGSDNVMSTSVNLSSSQPGCQHPATSFEFQSGSLPTEHYFGRARAYPAGGVSMDPIMSSSMHAADGTGTVEYEQCAPSMGRRPSYAMRSNTSEPNLNSMIMRRLSNRPMEQPHAFGYTQPRNQMISISNRYHPQPHAGMGGSDMGSKYAPPPVATNQIILKSSFSDQNLHNSIVYEPGGMGGGAGGQNICISNKNVYDPHTGYQNQTITCISNSRENLGESNFRYTSNPEGTVCEINAPDVQFRSSRASNLYEEVAMPLDQARVPVKETLSAGRNIDCDEHEYGVSDLEMHPDHILNKPIEVLKSPSEYRYSAYPKKVTEDAYVVSSDVRGKPEKISRSHSLIAQDQIIDIEYDSDTGWKTKSVRKNVFASSVEEKVYRKRTRSLGGGNSEEETKGRRDSQCEMKKITPVQGRKSRSGSKASLDRSNLTLNIVKNVDAEESSRNQSEKGKSKNIEKPTATGKQGEKEKCTAVAKKEKESSKVGKTSSTSKPGVVKSKSSTGSSTPKKGTKSPAKSGSGSIKKAGSLKGKQIKTGGSTPSIASSSKVKVTEKPNFKEIDTKKSKSKLPCKKTDSKSRVDEIKSESTVLYSSSESIKSIIDEVRMELLQKPKSTVYLSDESPTVLNRSHSFSDGELNYSKRIHDSYDKYEFVDGRKHYLLKDERLKQRLTDRYGHERAVGYERSTDRRRGDDEYERRGCRMVPDGEEDDPRRRPEMFAKCKSRSSSLVSNDAGEIPLRRKVYHSDSAEEEDDDDGREDEEEGDDEVFESSFSRGRNSSFNGKSSLKRRARYDTRRTSSLEGLDQFLANLQDRNRRGGRDGAGKNDSVRHSSVSINEKPEYFEYTKSPSSPYCTSSSGRGSASSRKPANYASTLSSNKSSNGVALLQTTPKRPSMKKSSNMPPAVGDVVGRASDGTRTRSGSPSTSAGRLPVDHQRRARTTHSDYDVRGRSRYGGHNGGREAYRQRDRDRDRDRNSSDHERDRDLSDREQRESRERGELDQSLSNTEGTPEDKIDGSLSDTAVIQSLDARRKLDQRSPKSETPTRDRDRFGTGMGIKSNSTSQLSATGRKRRMGFGKRGKNSFTVHRSEEVLPGEVRGGGGLSRGSSASSDGEGSADGDSTPRVASKRVDRVTSGAPAHKCTMLPPHSDRLVHQILSSPSVAHSILLADQGVSPSSSSSTSQRKRASAGSIQRSVEVTPIPAQSAAYRAGSLHSISSSEGSSWSPSLRVAGDTGPLSDFIDGLGPGQLVGRQVLGAPALGDIQLSMCYQKGSLEVEVIRARGLQARPGSKVLPAPYVKVYLVSGKKCIEKAKTTTARKTLDPLYQQQLVFREPFAGCILQVTVWGDYGRMEGKKVFMGVAQIMLDNLNLSHIVIGWYKLFGTTSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VCJ2</accession>
    <name>A0A6E8VCJ2_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">dihydropyrimidinase</fullName>
            <ecNumber evidence="4">3.5.2.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>5,6-dihydrouracil + H2O = 3-(carbamoylamino)propanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16121"/>
            <dbReference type="ChEBI" id="CHEBI:11892"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15901"/>
            <dbReference type="EC" id="3.5.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the metallo-dependent hydrolases superfamily. Hydantoinase/dihydropyrimidinase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.2.2" evidence="4"/>
    <dbReference type="RefSeq" id="XP_049463853.1">
        <property type="nucleotide sequence ID" value="XM_049607896.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VCJ2"/>
    <dbReference type="EnsemblMetazoa" id="ACON003124-RA">
        <property type="protein sequence ID" value="ACON003124-PA"/>
        <property type="gene ID" value="ACON003124"/>
    </dbReference>
    <dbReference type="GeneID" id="120947547"/>
    <dbReference type="CTD" id="40675"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_014478"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003124"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042989"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004157">
        <property type="term" value="F:dihydropyrimidinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006208">
        <property type="term" value="P:pyrimidine nucleobase catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000076">
        <property type="entry name" value="Dihydropyrimidinase like 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="entry name" value="Urease, subunit C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050378">
        <property type="entry name" value="Metallo-dep_Hydrolases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11647:SF1">
        <property type="entry name" value="COLLAPSIN RESPONSE MEDIATOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11647">
        <property type="entry name" value="HYDRANTOINASE/DIHYDROPYRIMIDINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="5">
        <location>
            <begin position="72"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00036696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00039113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003124-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="333" mass="36401" checksum="31F96652A3C18870" modified="2020-08-12" version="1">MASPTPAPVKKVPIHLQSAQNRLYIKRGHVVNHDGMQQADVYIEDGTVRYVGSGADFVVPGGCRTIDATGKLVMPGGIDPHTHFQLEFGGTVSVDDFYKGTKAAVAGGTTTILDFVLPKKGESLLEAYDAWRARADPKVVCDYGLHVGITWWSKSVRDEMRILCQERGVNSFKCFMAYKGLYQLNDSELYEVFETCKELGAVAMVHAENGDIIAKNVTKLLTEGVTGPEGHELSRSEEVEAEATNRACVIAHQAHCPLYVVHVMSKSAGIEVARARRRYNGTGIFGETLAAALGTDGTHYHDKCWHHAAAHVLSPPLRPDPTTPEFLMKLLAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VCL2</accession>
    <name>A0A6E8VCL2_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_040221902.1">
        <property type="nucleotide sequence ID" value="XM_040365968.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VCL2"/>
    <dbReference type="EnsemblMetazoa" id="ACON003206-RC">
        <property type="protein sequence ID" value="ACON003206-PC"/>
        <property type="gene ID" value="ACON003206"/>
    </dbReference>
    <dbReference type="GeneID" id="120949027"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010338"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003206"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037727"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008028">
        <property type="term" value="F:monocarboxylic acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17352">
        <property type="entry name" value="MFS_MCT_SLC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000271">
        <property type="entry name" value="Monocarboxylate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000372">
        <property type="entry name" value="Monocarboxylate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF286">
        <property type="entry name" value="GH22266P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="92"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="121"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="147"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="179"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="211"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="550"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="585"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="615"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="639"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="677"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="705"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="318"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="769"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="323"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="362"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="772"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="783"/>
            <end position="792"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003206-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="819" mass="88045" checksum="4F4CAF0DF4AAA45D" modified="2020-08-12" version="1">MTSKASKTTTDSEIACEEAARLTADTQEYTSPDDDDGGCEYHDMPPPPDGGYGWVIVFASFMCNMIVDGIAYTFGVFLNEFVVYFGEGKGTVAWVGSLLSGMYLSAGPVVSALANKYGCRAVCIAGSIISCAAFALSTLSTSVTMLMLTYGVMGGIGFGLIYLPAVVAVGYYFETKRSLATGIAVCGSGFGTFAFAPLANMLLENFDWKNSNLILAGLILNCAVFGAMMRPLTYPKEDKVKPLMQRMYEEKRLQMERGSIGGSYFMVQLPDGTMEKRLKAPLNADPGVHSSLALDQLAAQQGGMHPVATLPTISEFKAQEQNGSSGSSSESSQIEMKKPLNKKRNTNSESDAADYGADNMPRNASQPAFTSHQSGMPKNGSVPTFDRVRKHSTGERFKPSLAAIKASSRGDVGSNGDVRKSMHLRLSRGSVNGSKNNNAEEFDDGSMFTSKASLKADRPAMVRPLSRKDIFYSGSVTNLKEFQSQKSLTNYRNSVVSLTKFEKEHRNDVRDDVEKGREEQYDLCPCLALPESFKNAIGAMMDVSLLRDPVFMMIGVSNIFGMAGLYVPFVYLVDAAVLDGIEQNSASFLISIIGITNTVGRIVCGYVADFPQVDALFLNNICLVISTVAVALTPFCHSYAAYVAMAIAFGIAIAGYISLTSIILVDLLGLDKLTNAFGLLILFRGAATIVGSPLAGALYDATQSYSIPFFVAGGLFALSAITSFAAPAMKRFRKPSEAPVHVEVLTPIDEEPSEDLADDDQPITMVPKIIQTAPSPSTEQPVTSNITSTTTINDDRKQPQSNGSAKDTDKEVSQMESVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VCL9</accession>
    <name>A0A6E8VCL9_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="3">N-myc downstream regulated</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NDRG family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VCL9"/>
    <dbReference type="EnsemblMetazoa" id="ACON003238-RA">
        <property type="protein sequence ID" value="ACON003238-PA"/>
        <property type="gene ID" value="ACON003238"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004640"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003238"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033801"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004142">
        <property type="entry name" value="NDRG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11034">
        <property type="entry name" value="N-MYC DOWNSTREAM REGULATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03096">
        <property type="entry name" value="Ndr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="220"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="223"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003238-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="269" mass="29797" checksum="45D1F7C3A4AD363B" modified="2020-08-12" version="1">MSSAQDRRASFARRAESLMEKRYAVNTEKSGEIIVTVQGDLSQQEKRAVFLTVHDLGTNHSSFEEFVNSACMIEIKERSCFIHIDVPGHADNAPNLADQLVGDNPDKEKIVSEFQSRLHSSLNSKNLKQYVKAFMSRKDLPLKNCKVDCLLITGIMSPYASVVEKLYKDLNKEKVTLLKVERAGDVLADAPAKVAQSILLFCKGQGLLTSVAMPGVDRNRAFSTSSSGSTEGSTGARRLSRGMSMEDYDKPNIRRLSVTINEQLPPIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VEN7</accession>
    <name>A0A6E8VEN7_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Innexin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">inx</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Structural component of the gap junctions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Gap junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 12">Cell membrane</location>
            <topology evidence="2 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the pannexin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VEN7"/>
    <dbReference type="EnsemblMetazoa" id="ACON001487-RB">
        <property type="protein sequence ID" value="ACON001487-PB"/>
        <property type="gene ID" value="ACON001487"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012136"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001487"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030923"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005921">
        <property type="term" value="C:gap junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005243">
        <property type="term" value="F:gap junction channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034220">
        <property type="term" value="P:monoatomic ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000990">
        <property type="entry name" value="Innexin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11893">
        <property type="entry name" value="INNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11893:SF40">
        <property type="entry name" value="INNEXIN SHAKING-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00876">
        <property type="entry name" value="Innexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01262">
        <property type="entry name" value="INNEXIN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51013">
        <property type="entry name" value="PANNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0965">Cell junction</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0303">Gap junction</keyword>
    <keyword evidence="11 12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
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</entry>
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        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
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            <authorList>
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                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
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                <person name="Galvin B.D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain>Mali-NIH</strain>
        </source>
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                <consortium name="EnsemblMetazoa"/>
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</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
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            <taxon>Nematocera</taxon>
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            <taxon>Anophelinae</taxon>
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                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
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            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
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    <dbReference type="Proteomes" id="UP001105220">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
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        <location>
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            <end position="28"/>
        </location>
    </feature>
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            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="253" mass="29513" checksum="DAF3D588DB936586" modified="2020-08-12" version="1">MGKHDMKRGISQRSSDAGESPKHYASTNLVGKFTQSVRRIVQDVKDEGSPSGMSREELLETNERLRAVRLRLEESYDTAKKALVNLMNKYGDSKSQRNIFNRYPMLKLMIKDVIRLETQYWTLVEIPKQEKLETVPAFVLRACSIMEKSQKSGEGVKTSAKLAEEAAERRERMERLETMTTAQIEQENTQMINDLYRLLKKYTGLRNLIRELKSEYGNSKIYPIFPRYTMLKDMIKDIMHDPDYMEVCHEVDN</sequence>
</entry>
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    <accession>A0A6E8VES2</accession>
    <name>A0A6E8VES2_ANOCL</name>
    <protein>
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        <name type="scientific">Anopheles coluzzii</name>
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            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
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                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
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            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
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        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
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            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
            <location evidence="1">Basement membrane</location>
        </subcellularLocation>
    </comment>
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    <dbReference type="Proteomes" id="UP001105220">
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    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="CDD" id="cd22593">
        <property type="entry name" value="Kunitz_conkunitzin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Kunitz_papilin_lacunin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="WAP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="A disintegrin and metalloproteinase with thrombospondin motifs 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ADAM metallopeptidase with thrombospondin type 1 motif 9"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Collagen, type VI, alpha 3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.830">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.410.10">
        <property type="entry name" value="Pancreatic trypsin inhibitor Kunitz domain"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013273">
        <property type="entry name" value="ADAMTS/ADAMTS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050439">
        <property type="entry name" value="ADAMTS_ADAMTS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010294">
        <property type="entry name" value="ADAMTS_spacer1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036645">
        <property type="entry name" value="Elafin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002223">
        <property type="entry name" value="Kunitz_BPTI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036880">
        <property type="entry name" value="Kunitz_BPTI_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010909">
        <property type="entry name" value="PLAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020901">
        <property type="entry name" value="Prtase_inh_Kunz-CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008197">
        <property type="entry name" value="WAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723">
        <property type="entry name" value="ADAMTS A DISINTEGRIN AND METALLOPROTEASE WITH THROMBOSPONDIN MOTIFS PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723:SF281">
        <property type="entry name" value="PAPILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05986">
        <property type="entry name" value="ADAMTS_spacer1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00014">
        <property type="entry name" value="Kunitz_BPTI"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08686">
        <property type="entry name" value="PLAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19030">
        <property type="entry name" value="TSP1_ADAMTS"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00090">
        <property type="entry name" value="TSP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01857">
        <property type="entry name" value="ADAMTSFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00759">
        <property type="entry name" value="BASICPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00131">
        <property type="entry name" value="KU"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00217">
        <property type="entry name" value="WAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57362">
        <property type="entry name" value="BPTI-like"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57256">
        <property type="entry name" value="Elafin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00280">
        <property type="entry name" value="BPTI_KUNITZ_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50279">
        <property type="entry name" value="BPTI_KUNITZ_2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50900">
        <property type="entry name" value="PLAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51390">
        <property type="entry name" value="WAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0084">Basement membrane</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="9 10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="4" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0722">Serine protease inhibitor</keyword>
    <keyword evidence="5 12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026103435" description="Papilin" evidence="12">
        <location>
            <begin position="21"/>
            <end position="2443"/>
        </location>
    </feature>
    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="13">
        <location>
            <begin position="1480"/>
            <end position="1530"/>
        </location>
    </feature>
    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="13">
        <location>
            <begin position="1539"/>
            <end position="1589"/>
        </location>
    </feature>
    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="13">
        <location>
            <begin position="1598"/>
            <end position="1648"/>
        </location>
    </feature>
    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="13">
        <location>
            <begin position="1658"/>
            <end position="1715"/>
        </location>
    </feature>
    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="13">
        <location>
            <begin position="1724"/>
            <end position="1774"/>
        </location>
    </feature>
    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="13">
        <location>
            <begin position="1808"/>
            <end position="1860"/>
        </location>
    </feature>
    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="13">
        <location>
            <begin position="1871"/>
            <end position="1921"/>
        </location>
    </feature>
    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="13">
        <location>
            <begin position="1949"/>
            <end position="1999"/>
        </location>
    </feature>
    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="13">
        <location>
            <begin position="2008"/>
            <end position="2058"/>
        </location>
    </feature>
    <feature type="domain" description="WAP" evidence="16">
        <location>
            <begin position="2144"/>
            <end position="2189"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="2207"/>
            <end position="2298"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="2302"/>
            <end position="2389"/>
        </location>
    </feature>
    <feature type="domain" description="PLAC" evidence="15">
        <location>
            <begin position="2395"/>
            <end position="2434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="696"/>
            <end position="1103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1233"/>
            <end position="1263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="707"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="767"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="845"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="898"/>
            <end position="923"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="930"/>
            <end position="985"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="992"/>
            <end position="1057"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1058"/>
            <end position="1067"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1079"/>
            <end position="1089"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1253"/>
            <end position="1263"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="64"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="68"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="79"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR613273-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001662-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="19">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2443" mass="262896" checksum="9E8599AF03E4BA63" modified="2020-08-12" version="1">MRYTKLLVLLVTLQLSFTESRRFGARHKRQHGSNLYQPESFIIPGGGEGPPHERWSAWGAPSECSRTCGGGVAYQERECLDLDHTGAPVCTGGKRKYFSCNTQDCPERELDFRKQQCSEFNDVPFEGHRYQWVPYTRAPNPCELNCMPVGERFYYRHRAKVADGTRCNDESFDVCVDGTCQPVGCDMMLGSQAKEDKCRICRGDGSSCKTASGLLDASNLQVGYNDLLLIPAGATNVLISERAPSNNYLAIRNLTGFYHLNGNYRIDFPRTIHFAGSDWHYERRPQGFAAPDRLTCLGPTTESVYLVLLSQDRNVGVNYEYSVSSKSAPVDEPDSYSWMYTAFEPCTVTCGGGVQTRNVTCNSKSSLKEVDEGLCNLGEKPPTTQKCGQQSCPPRWFEGPWSNCSKPCGTEGKQTREVYCERVSADGETKKIEDDVCLKQVGNKPATERECNQGIICPEWYVGRWSPCNKLCGEGERTRKVVCYRKENGRITVLEDEECITEKPAVSEQCMLRPCEGVDYVTSSWSGCDECGATVETRTVHCASKSGTVYDASFCADRQLPELRRECKLTPCEYQWYTSQWSKCSAVCGKGVQTRTVVCGVFDGQSLKRADDDYKCDPEQKPEKEQECEGPPECPGQWFAGPWTDCDKGCGGGVMSRKVLCLANGTVVPETNCDVDKIQLATESCNKHDCTEDEVIPVEVGSKQPEVEDDYDEDELCEEEDDEEDASGDASGDGEEDGSGAVSTEGQDGVMMVTEDTSLAEGIELDSSVSGTTESSLETDEMMMSDATGFDTGATDPDEGSTTEATVEGSGDGSSPMSEVSEGSGDEGEATTVVAASRASDDASSDAAGSTTEASASSGSSMASESSGSTEASAESSTESSDMASTSQASSVEDSMPTTTDSGVSTTDASDSTTDVTETSTSSTDEESTTDLSSTTTMTDLTSTMDDASTTDVPSTTDSSEAASSTVASDTATSSSSSDGSTTEVSSDESSTDSSTKMVEGSESTETSVMSSSTEPSVTGTSVDSSSESGATEESATGASTDGSSTTTVDSTEPTVTGASTEDFTGSTVTEETVDETTFEIWDSSTDASTTDDEADDRESSTPYTLTSIIAKEQKPRKCKPRPKVPQCAKSTHGCCPDGKTKASGPFLEGCTLAETCKETKHGCCPDGVSPAKGPNGKGCPKAECADTLFGCCPDKVTPAEGNDAEGCPVETTTMPPGCTAAKHGCCPDGTTEAKGPKAKGCPGVEKEEEEAPAGTEQPTPEELPAAVEGCATTPHGCCPDNATAASGPDGQGCEPCSREPFGCCPDGKTPAHGHSGEGCCLQTPYGCCPDNVVAARGPNLEGCDCQYAPYGCCPDNRTSARGHDNEGCGCQYARHGCCPDKETEATGPSFEGCPCHAHQFGCCPDGVTAAKGPHNQGCHCTHSEFKCCGDGKTPAKGPDGEGCTCADSKYGCCPDGVSEAQGSKFEGCTAVPESPQKACALPKDKGPCHNYTVKHFFDVEYGGCGRFWYGGCEGNNNRFDSAEECKNICETPTGKDVCHLPKISGPCTGHYNMWYYDAERNMCAQFTYGGCLGNANRFESQEECKALCSVDDSKPPCEQPMEAGPCNGTFERWYYDKETDACHPFYFGGCKGNKNNYPTEASCGYHCKKPGVHKPSCSEPLDTGSCEAQQQQQQQQQARWYFASDSQKCMPFYFTGCGGNDNQFVSRDHCEEQCPPKVEKDICFLPAEIGECQNYTAHWYFDTKDERCRQFYYGGCGGNGNNFADEQSCINRCIEEGPKPAPAPPGAAPVAPVQPAAPGKPFDRSRCQLPMDNGDRECSPYEARYYFSTQTGACELFTYTGCGGNGNNFQRKEECERSCGSEGQGEQDVCQLPHAYGECSGNEERWFYEPHEQRCVRFAYSGCGGNGNNFASEAECERSCPAGGRAPVDVDVRGPHEQQPAAGNGSRCEEVPDYGDGDGDEILFYYNAERQSCERFRYSGAGGNANRYRSEEECERVCGMYRGVDVCQDAVQAGQCTESIPMYYYDSRTHACYAFNYSGCEGNGNRFASAEECEGTCVHQRPGFDDAANAVNPCEQYDHECSQLHCPYGIAKSYDPANGCERCQCNDPCAGYFCPAGSECVVDVQSGGAFVGTGSEFVGVCRESQKPGDCPVLANATHCSRDCNTDADCRGNNKCCQAGCALICISPVDRASVDRPTGGQPGVPGPTVLEEVPQEELDIKSEEGGIALLRCFATGFPPPSIRWRKGEIMLNTNQGRYVITSDGDLQIVQLHRTDSGTYVCIADNGIGEPALREVQLTVNAPVSVQMHFPQGRDLRPNSNFTINCTVDGYPRPTVNWFKDGQMLVPTDRIHITDAHQLMVFGAIPSDTGRYKCLARNEMSEAFQENSIHVEGVYVPPGCTDNQLLAKCDLIVAGHYCNHKYYARFCCRSCTLAGQINVNNRYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VES9</accession>
    <name>A0A6E8VES9_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Amidase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_049461535.1">
        <property type="nucleotide sequence ID" value="XM_049605578.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VES9"/>
    <dbReference type="EnsemblMetazoa" id="ACON001699-RB">
        <property type="protein sequence ID" value="ACON001699-PB"/>
        <property type="gene ID" value="ACON001699"/>
    </dbReference>
    <dbReference type="GeneID" id="120953163"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006203"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001699"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041091"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052739">
        <property type="entry name" value="FAAH2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43372">
        <property type="entry name" value="FATTY-ACID AMIDE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43372:SF2">
        <property type="entry name" value="IP13792P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001221">
        <property type="entry name" value="Amidase_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026337531" evidence="2">
        <location>
            <begin position="29"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="domain" description="Amidase" evidence="3">
        <location>
            <begin position="64"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="1">
        <location>
            <position position="127"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="1">
        <location>
            <position position="202"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="1">
        <location>
            <position position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR001221-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001699-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="519" mass="57550" checksum="49D416949C34EBDD" modified="2020-08-12" version="1">MSYAIRRLLRAAMCVFSWFVMPYSQCVSGRIERRKLPPIEDPLLLLSATVLAERIRKREIRSEDVVRAYIQRCQQVNPLLNAIVEDRFEAALEEAQEVDRQLAKGTLGPAEELARTKPLLGLPVSIKESLAVEGMSNTAGRKLREKKVALSDAPVVHQIKRAGGIVLLVSNTPELCLCWETYNQCTGLTRNPYNLQRTAGGSSGGEAALIAAAGSLLGVTTDIAGSSRLPAMFTGVFGHKPSPYVVSPYGHHPSCDDENWGSFFTPGAMCRYAEDLPLLLEAMRDPEGTPVTLHKPVPIGALKCYYMENDGPSGLTRPIDADIVQAIRDVAAHLNAQRVNLKRLRWTLDISVCKMLRMKNVETIYSPQANGKPDTTMKREVFKYLLGMSKSDLPSVMIGPMQHIVNNYIPQSRLDFLDAQTEKLRKDFIDLLGTDGVFIYPGFPNTAHRHYRIFHKLVDTTYMMVFNTVGLPAASCMVGFDREKLPIGVQIVAAPGQDHLIFAVAKELERRFGGWVAPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VF25</accession>
    <name>A0A6E8VF25_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Iso_dh domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040219131.1">
        <property type="nucleotide sequence ID" value="XM_040363197.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VF25"/>
    <dbReference type="EnsemblMetazoa" id="ACON002192-RA">
        <property type="protein sequence ID" value="ACON002192-PA"/>
        <property type="gene ID" value="ACON002192"/>
    </dbReference>
    <dbReference type="GeneID" id="120947668"/>
    <dbReference type="CTD" id="3421"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_013383"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002192"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041674"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006102">
        <property type="term" value="P:isocitrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.718.10:FF:000001">
        <property type="entry name" value="Isocitrate dehydrogenase [NAD] subunit, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.718.10">
        <property type="entry name" value="Isopropylmalate Dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004434">
        <property type="entry name" value="Isocitrate_DH_NAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00175">
        <property type="entry name" value="mito_nad_idh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835">
        <property type="entry name" value="DECARBOXYLATING DEHYDROGENASES-ISOCITRATE, ISOPROPYLMALATE, TARTRATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835:SF60">
        <property type="entry name" value="ISOCITRATE DEHYDROGENASE [NAD] SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53659">
        <property type="entry name" value="Isocitrate/Isopropylmalate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="3" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Isopropylmalate dehydrogenase-like" evidence="6">
        <location>
            <begin position="51"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002192-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="384" mass="42407" checksum="58C6F243A200CDC0" modified="2020-08-12" version="1">MALRLFRLSQDVVTPLIKRNCTVSAFELQHKNPVQRKVEQIPKAQYGGRHTVTMLPGGGIGPELMSYVREVFRFAGVPVDFEIVDIDPASEGNDDLEYAITSIKRNGVAIKGNIETKSEATGILSRNVALRNELDLFVNVLHCKSFNAIPAHHQNVDVVIVRQNTEGEYAMLEHESVRGVVESMKVVTIENAARVARFAFEFAKNNNRKKVTTIHKANIMKLADGLFLKVARDIAKEYPDIQHNDMIIDNCCMQLVSNPHQFDVMNTTNLYGSITSNVLCGLVGGAGLFSGRNYGDHYAVFEPGTRNTGTAIAGKNVANPVAMLNAAVDMLYHLGHRYHADCISDALHKTIDIDGIHTPDLGGNNTSTDIVQNVLKHLAEKRTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VF27</accession>
    <name>A0A6E8VF27_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PID domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VF27"/>
    <dbReference type="EnsemblMetazoa" id="ACON002199-RB">
        <property type="protein sequence ID" value="ACON002199-PB"/>
        <property type="gene ID" value="ACON002199"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000820"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002199"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031171"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21219">
        <property type="entry name" value="FI19613P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21219:SF4">
        <property type="entry name" value="PID DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="168"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="444"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="511"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="722"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="849"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="223"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="446"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="484"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="722"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="750"/>
            <end position="775"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002199-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="870" mass="92572" checksum="10F9EEBB93CA5852" modified="2020-08-12" version="1">MNFHTLNFSLPHTHTTQTGGSLDISPIGLRVRAGSGKEPSVTPFQNIAVWSAVKFVVSGAEGGAAFLPLITDPENIDKSTLFRPLSAADKRRLSSGIHSPLFAIVMRSTSVPRQLECHGFVCQTPEDAIVIAATLYQSLMAHMSSGDNQRPKRPKNRNGVSCMSIASSTVTTNTHLPGGSTKLSSRRSSASQRSMLPPPPRPPRMKRSATSSLSGESDAVAGPHEDESSTEERRKKNVKSKRPPPIPPNPPRAISNRPLLDEIYTDRSHSTDEQGVAGKEGPYPALTDNSTGDILTRVAIPRSGSFLNTAGLTRYKSRATRRHTGKVGGGGGSPLGFSELFNEFRLQENLHSLDEILNAIINSDGMSFNDLKPIYKEFLLKLAVTLTKDELYQRSKSIMRRQKKKKLKRRNSNVQVSWEARRKLLIGAKGLKKVFRFGKFRSKKSAGGGGGAGGSHGTGGSHGVGGGRAGNGGAGHAAAAAAGHGTGGHGSAPGGRAHQLADQLSLDLKAAGKKDRSRIATSGSEVSDARHEHTLTAHNRNSSSGYVSCSECSYDSDACTCASADRCYCSLGGDDINAKLHQASSRSSAPSCRSEDKCYCSMGETPNEEGSTTWCDTDSCISNEKCYCSTRHGQVAKCAAAGSSGSASKGTIAGVHGQKHCAAKPAKVDKLALDYELFTVGGSGRPVKASEALSVKKTVEVAAEFADVKLSQTTDITNLKADAQSSATSGSSSLVSTAKKSADHRGASAGRHHGHNHHHTHHAHRSEQRNHHHRPQPALGNGGSSGASSTSNLHYHRHHYSTSTKKKGLPVQLPGNDYQYIHSTQSELIVRGAKQELKTPKRLTKSESYYQAGRPVSSSLGLEDSLGYLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VGR9</accession>
    <name>A0A6E8VGR9_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">long-chain-fatty-acid--CoA ligase</fullName>
            <ecNumber evidence="7">6.2.1.3</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a long-chain fatty acid + ATP + CoA = a long-chain fatty acyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15421"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57560"/>
            <dbReference type="ChEBI" id="CHEBI:83139"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:15422"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.3" evidence="7"/>
    <dbReference type="RefSeq" id="XP_049462370.1">
        <property type="nucleotide sequence ID" value="XM_049606413.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VGR9"/>
    <dbReference type="EnsemblMetazoa" id="ACON003623-RB">
        <property type="protein sequence ID" value="ACON003623-PB"/>
        <property type="gene ID" value="ACON003623"/>
    </dbReference>
    <dbReference type="GeneID" id="120953788"/>
    <dbReference type="CTD" id="46068"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_008993"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003623"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035053"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090433">
        <property type="term" value="F:palmitoyl-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035336">
        <property type="term" value="P:long-chain fatty-acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000026">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272:SF83">
        <property type="entry name" value="ACYL-COA SYNTHETASE LONG-CHAIN, ISOFORM J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="9">
        <location>
            <begin position="124"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="10">
        <location>
            <begin position="590"/>
            <end position="631"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00026121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003623-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="714" mass="79931" checksum="17270CC13C8157BD" modified="2020-08-12" version="1">MTHKQKDMESVWVQSAIGAIKIITMVYDIITMPIYLAIQRPWKRRQLARRVKAKIIQQDSSSITYRSVDSPGEMHVKMMQNNIDTLERMFNFVTKVHTTKRCIGTRQILGEEDEMQPNGRMFKKFRMGDYVWRNFIETEHAAACFGRGLRELGQEPKQNIVIFAETRAEWMIAAHGCFKQNMPVVTIYATLGDDGVAHGINETEVTTVITSHELLPKFKSVLNVTPNVKKIIFMEDQLHPTDTTGFKEGVEIIPFSKVIEIGNTSTIPGSPPAAEDTAIIMYTSGSTGTPKGVLLSHANCIGTMKNFCDIFKIYPDDVLIGFLPLAHVFELLAESVCLLTGVPIGYSTPLTLIDSSSKVMKGCKGDASVLRPTCMTSVPLILDRISKGINDKVNAEAPMKKAFFKFAYNYKSKWTARGYQTPLMDKILFKKIAKLLGGRIRSVLSGGAPLAPDTHEQIKLCLCVDVIQGYGLTETTAGAAVMDKWDMEYSRVGAPSSSNDIRLINWEEGNYRVTNKPYPQGEIVVGGTTVSKGYYKLPGKTQEDFFEEDGQRWFRTGDVGEIHPDGALKIIDRKKDLVKLQAGEYVSLGKVESELKTCPVVENICVYGDSTKQYTVALVVPNPKHLEEIAERLDIRGVEFEDLCDNKKLEKAVLQELADHGRKCKLHRSEIPAAVHLCKDIWTPDMGLVTAAFKLKRKDIQERYQAEISKMYAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VGU4</accession>
    <name>A0A6E8VGU4_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Tyrosine-protein kinase</fullName>
            <ecNumber evidence="9">2.7.10.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the protein kinase superfamily. Tyr protein kinase family. Fes/fps subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.2" evidence="9"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8VGU4"/>
    <dbReference type="EnsemblMetazoa" id="ACON003651-RA">
        <property type="protein sequence ID" value="ACON003651-PA"/>
        <property type="gene ID" value="ACON003651"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004928"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003651"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032760"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004715">
        <property type="term" value="F:non-membrane spanning protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002009">
        <property type="term" value="P:morphogenesis of an epithelium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07657">
        <property type="entry name" value="F-BAR_Fes_Fer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05041">
        <property type="entry name" value="PTKc_Fes_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10361">
        <property type="entry name" value="SH2_Fps_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000212">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000064">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000089">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000051">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031160">
        <property type="entry name" value="F_BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001060">
        <property type="entry name" value="FCH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035849">
        <property type="entry name" value="Fes/Fps/Fer_SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050198">
        <property type="entry name" value="Non-receptor_tyrosine_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016250">
        <property type="entry name" value="Tyr-prot_kinase_Fes/Fps"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24418">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00611">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000632">
        <property type="entry name" value="TyrPK_fps"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00055">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51741">
        <property type="entry name" value="F_BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 13 15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 9 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="2 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 9" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="F-BAR" evidence="19">
        <location>
            <begin position="1"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="17">
        <location>
            <begin position="386"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="18">
        <location>
            <begin position="489"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="329"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="77"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="341"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="358"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="612"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="495"/>
            <end position="503"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 14">
        <location>
            <position position="519"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00051245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR000632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000632-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000632-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003651-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="750" mass="85371" checksum="33B5A51809EEC6A6" modified="2020-08-12" version="1">MGFSSALQGRAAHDALLNRQEAELKLLETMKRCLAQKAKCDREYAVSLAAVTQQGLKIDRSDDLQGNHVMRAWRSFMEELDHTAKQIRTNAEQLETVCHEKLASLYQEKRRVRKQYQEEHTKIATQFSHLTEDVARKKSEYQKHLDYYKLLRGRFEEHIKSGRSGRKLDDVIDKYQKACRKLHQAHNEYVLLITEAVEVEKDFRTILLPGLLEHQQSLQEGFIQAWSNLLQEIANLSDMTSDKFVDIQRRIESSIAGINSTEEYREFTEKHKTSPTPPVIFQFDESLVEDSLGKLQANTLTVDNLTVDWLRGRQTELEGTIKDLQDRQSKLCGEPNGTNGATGSSPAAVSPASTPGTKPSTPILNGSSTSNGMTLSTNRPLHEEEWFHGVLPREEVVRLLRNEGDFLVRETTRNDESQTVLSVCWNGHKHFIVQTTAEGHYRFEGPAFPSIQELIVHQYQSELPVTGRSGAVLRKPVLRERWELSNDDVILLDKIGRGNFGDVYKAKLKSSKNTLVAVKTCRMTLPEEQKRKFLQEGRILKQYDHPNIVKLIGICVQKQPIMIVMELVAGGSLLMFLRKNASTLGQRQMMGMCRDAAAGMRYLESKNCIHRDLAARNCLIGSENIVKISDFGMSREEEEYIVSGGMKQIPIKWTAPEALNFGKYTSLCDVWSYGILVWEIFSRGDTPYSGMSNSMARERIDEGYRMPAPEGAPPEMYRLMLKCWSYEPESRPHFDEICSVVDALLLCTKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VGW1</accession>
    <name>A0A6E8VGW1_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">PRA1 family protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Secretory vesicle</location>
            <location evidence="2">Synaptic vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the PRA1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VGW1"/>
    <dbReference type="EnsemblMetazoa" id="ACON003662-RA">
        <property type="protein sequence ID" value="ACON003662-PA"/>
        <property type="gene ID" value="ACON003662"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000134"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003662"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030183"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008021">
        <property type="term" value="C:synaptic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004895">
        <property type="entry name" value="Prenylated_rab_accept_PRA1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19317">
        <property type="entry name" value="PRENYLATED RAB ACCEPTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19317:SF0">
        <property type="entry name" value="PRENYLATED RAB ACCEPTOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03208">
        <property type="entry name" value="PRA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="58"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="109"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003662-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="166" mass="18714" checksum="B3C9E1AE92E6A4A2" modified="2020-08-12" version="1">FTNFSTRIPSLWELLRLSRQNIRPWSEFLQTSNFKTVANVSRLTNRIIRNLAYFQSNYLFVFLGLIVYCLLTSPLILIVLGAVFYACYKIKQNSTPVAFFSKQLNTNQQCIAVGVASVPVLYLAGAGAVMFWVLGASFFVISLHAAFYNIDAIVTEDTETFLSETV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VGW2</accession>
    <name>A0A6E8VGW2_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VGW2"/>
    <dbReference type="EnsemblMetazoa" id="ACON003660-RB">
        <property type="protein sequence ID" value="ACON003660-PB"/>
        <property type="gene ID" value="ACON003660"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009151"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003660"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038023"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029201">
        <property type="entry name" value="Jiraiya"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39947">
        <property type="entry name" value="IP19862P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39947:SF1">
        <property type="entry name" value="IP19862P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15038">
        <property type="entry name" value="Jiraiya"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="176"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="221"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="40"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="87"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003660-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="264" mass="26084" checksum="05237572E88528AE" modified="2020-08-12" version="1">MLKHIHTGPVTTGQNRSMRGAAGGGGGGGGGLGRPIPPLNVSTVSASSAATINSTNTGGPPPPIGTKHMNGGPPGRTLLSSSFMSPSTGNGTGNNNNNNNNTNTSNNNILHNASALSGLSVALGNGAIGGVGAGGGGGGGGAMGLAGFNHNNSTTNNNILSSTINMNKDRLGAREALTSLGLLCLVSLLLALLSLIFLLKISPGGRDPPPPTIGPAVLEDYAIVYDVTLALCALSLSLNLCCLLVCAIQFLFAVKLVRAPATTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VGW4</accession>
    <name>A0A6E8VGW4_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Complexin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Positively regulates a late step in synaptic vesicle exocytosis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the complexin/synaphin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040226636.1">
        <property type="nucleotide sequence ID" value="XM_040370702.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VGW4"/>
    <dbReference type="EnsemblMetazoa" id="ACON003509-RE">
        <property type="protein sequence ID" value="ACON003509-PE"/>
        <property type="gene ID" value="ACON003509"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON003509-RG">
        <property type="protein sequence ID" value="ACON003509-PG"/>
        <property type="gene ID" value="ACON003509"/>
    </dbReference>
    <dbReference type="GeneID" id="120951782"/>
    <dbReference type="CTD" id="64877"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_014259"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003509"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034498"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043195">
        <property type="term" value="C:terminal bouton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046928">
        <property type="term" value="P:regulation of neurotransmitter secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016079">
        <property type="term" value="P:synaptic vesicle exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.580:FF:000002">
        <property type="entry name" value="Complexin, isoform AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.580">
        <property type="entry name" value="Single Helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008849">
        <property type="entry name" value="Synaphin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16705">
        <property type="entry name" value="COMPLEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16705:SF4">
        <property type="entry name" value="COMPLEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05835">
        <property type="entry name" value="Synaphin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="4" id="KW-0532">Neurotransmitter transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="31"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003509-PG"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="142" mass="16528" checksum="5F029611A81687F5" modified="2020-08-12" version="1">MAAFVAKQMVGNKLNAVKGAVGGDGGDDENDKEKEEEAERERQEAIREAEERRKEKHRKMEEEREKMRQEIRDKYNIKKKEEIVEQAPQEEPNPLMRKKKTPEELAAEAEQEDLDDFTKLKNSIETQVNEIKTQIEGKCSLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VGW6</accession>
    <name>A0A6E8VGW6_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the DEAD box helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8VGW6"/>
    <dbReference type="EnsemblMetazoa" id="ACON003663-RA">
        <property type="protein sequence ID" value="ACON003663-PA"/>
        <property type="gene ID" value="ACON003663"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010291"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003663"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034566"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17966">
        <property type="entry name" value="DEADc_DDX5_DDX17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000079">
        <property type="entry name" value="probable ATP-dependent RNA helicase DDX17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 8" id="KW-0347">Helicase</keyword>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="12">
        <location>
            <begin position="214"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="245"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="11">
        <location>
            <begin position="452"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="101"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="7">
        <location>
            <begin position="214"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003663-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="555" mass="61035" checksum="B0DE1F18D87C1FE2" modified="2020-08-12" version="1">MWGQVCRRSLMTVVRSFCSTSSAASFGSRLRIPEVIAVPTTRRQSYCDRSRTLLPSAVLASTSRFYSTPISKEGFEDDLDMAPRFEREDYKKEPYHGVKRTGTEFKRGTGTTGSRPGGGGYGGGGGGGGAGGYGGGGSRFGGGGAGGGGGGYRNKWADAGGMTKIDWSKMTLAPFKKDFYHENSIVRNRSQKEVDRYLAKHDITLIGKCPKPITEFDEIEIPDYVKREIDRQGYKSPTPIQAQGWPIALSGLNMVGVAKTGSGKTLAYMLPAIVHINHQKPDPSVRGPLVLVLAPTRELAQQIQQVATEFGSSSYIRNTCLFGGSSKGPQASDLRRGVEIVIATPGRLIDFLESGTTTLQRVTYLVLDEADRMLDMGFEPQIRKILDHVRPDRQILMWSATWPKEVQRLARDFLGDYVQINVGSLELSANHNITQHVRVIAEKDKNPELGKLLEELYHEGNPGKILIFTTTKRQCDRISMQIKRYGYDSVSMHGDKSQQERERALGRFRNSSSCILVATDVAARGLEAEPSCCCGGDSEQKYISARCFTYVSPVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VH59</accession>
    <name>A0A6E8VH59_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Regulator of chromosome condensation</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VH59"/>
    <dbReference type="EnsemblMetazoa" id="ACON003742-RB">
        <property type="protein sequence ID" value="ACON003742-PB"/>
        <property type="gene ID" value="ACON003742"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012568"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003742"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037790"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051553">
        <property type="entry name" value="Ran_GTPase-activating"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058923">
        <property type="entry name" value="RCC1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45982">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45982:SF1">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25390">
        <property type="entry name" value="WD40_RLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00633">
        <property type="entry name" value="RCCNDNSATION"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00625">
        <property type="entry name" value="RCC1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00626">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="RCC1-like" evidence="5">
        <location>
            <begin position="1"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="42"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="94"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="211"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="265"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="319"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="376"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="376"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="416"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="428"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF25390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003742-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="463" mass="48763" checksum="45FA3F2E5C26F0D9" modified="2020-08-12" version="1">MGQLGMGEDVMEKTRPAVIEGLSDVVQISAGGMHNLCLTRHGTVYSFGCNDEGALGRDTSEEGSEFEPKQIDLPGQCVKISAGDSHSACLLDDGRVYAWGSFRDSHGNMGLTLEGNKRLPIEVLPGNRWVDIASGCDHLVLLSDIGHIYTVGCAEQGQLGRVSIRAASGESRRGKTQLLQPGIVTRRGKMIVAEAIWATTYCTFYKDYQTGRIFAFGLNNYCQLGIPNPSENVVKPVFVPEPTSFDEVVQIAGGQHHTLVLKTDHKVYAIGRKEYGRLGLGDDVTEDAKTLQPVAALGDKKVVSVCCGESTSFAVTDKGELYAWGMGSSLQLGTGLETDEAKPVLIASKQVAGKVILKASSGGQHSLFLVQETPAVTEKAPTKATAASKKTKPEAASTSNGQAKRSETKNGDEPEAASSSSSAAADSKPTTNGAAETTEASGSDEKAAPAKAAGGGGRKRKLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VH62</accession>
    <name>A0A6E8VH62_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="1">C2H2-type domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VH62"/>
    <dbReference type="EnsemblMetazoa" id="ACON003777-RA">
        <property type="protein sequence ID" value="ACON003777-PA"/>
        <property type="gene ID" value="ACON003777"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006377"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003777"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033237"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003777-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="92" mass="10930" checksum="9F7B90867907B1B0" modified="2020-08-12" version="1">MGVPTTVYIHTNIYNYSNKYKNLDRFQNDVKTFLKEYDRIVLHKDLYKLVFKSYLREIRSKVKILLQKFDATPLDDGSTRPEKEMMCCGVSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VH67</accession>
    <name>A0A6E8VH67_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Glucose-methanol-choline oxidoreductase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">Ngousso</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the GMC oxidoreductase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VH67"/>
    <dbReference type="EnsemblMetazoa" id="ACON003782-RA">
        <property type="protein sequence ID" value="ACON003782-PA"/>
        <property type="gene ID" value="ACON003782"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001505"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003782"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035524"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016614">
        <property type="term" value="F:oxidoreductase activity, acting on CH-OH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.560.10">
        <property type="entry name" value="Glucose Oxidase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012132">
        <property type="entry name" value="GMC_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000172">
        <property type="entry name" value="GMC_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007867">
        <property type="entry name" value="GMC_OxRtase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552:SF215">
        <property type="entry name" value="FI02019P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552">
        <property type="entry name" value="GLUCOSE-METHANOL-CHOLINE GMC OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05199">
        <property type="entry name" value="GMC_oxred_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00732">
        <property type="entry name" value="GMC_oxred_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000137">
        <property type="entry name" value="Alcohol_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00623">
        <property type="entry name" value="GMC_OXRED_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00624">
        <property type="entry name" value="GMC_OXRED_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucose-methanol-choline oxidoreductase N-terminal" evidence="4">
        <location>
            <begin position="11"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="Glucose-methanol-choline oxidoreductase N-terminal" evidence="5">
        <location>
            <begin position="187"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000137-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS00623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS00624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003782-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="497" mass="55679" checksum="F24D727F8F380923" modified="2020-08-12" version="1">MVNNRCNWPRGKVLGGSSVLNYMIYVRGNRNDFNHWESLGNPGWAYDDVLQFFVKSEDNRNPYLARNPYHGQGGLLTVQEAPWHTPLVAAFVEAGTEIGYENRDINGERQTGFMIAQGTIRRGSRCSTAKAFLRPIRLRKNLHIAMNSHVSKLVIDPETKHAVGVEFFRGGKRHYVRARKEIIMSAGSINTPQILMLSGIGPRAHLEDVGITTIQDLPVGENLQDHVGMGGLTFLVDKPVAILQNRLEAGSVTMNYVINERGPMTILGGLEGIAFVNTPFANVTDDWPDIQFHMAPASLNSDGGARVKKVLGLREDLYKEVFHPIEDTYSWTIMPLLLRPRSRGWVRLKSKNPFHYPLMNPNYFEDPFDAATLVEGAKIALRVGDAKVFKQFGNRLYRKPLPNCKQHKFLSDEYLDCQVRTISMTIYHPVGTAKMGPHWDPGAVVDPRLRVYGISGLRVIDASIMPTIVSGNTNAAVIMIGEKGAHMIKEDWLGHDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VH82</accession>
    <name>A0A6E8VH82_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 8">Glucose-methanol-choline oxidoreductase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the GMC oxidoreductase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VH82"/>
    <dbReference type="EnsemblMetazoa" id="ACON003785-RD">
        <property type="protein sequence ID" value="ACON003785-PD"/>
        <property type="gene ID" value="ACON003785"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004496"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003785"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040345"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016614">
        <property type="term" value="F:oxidoreductase activity, acting on CH-OH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.560.10">
        <property type="entry name" value="Glucose Oxidase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012132">
        <property type="entry name" value="GMC_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000172">
        <property type="entry name" value="GMC_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007867">
        <property type="entry name" value="GMC_OxRtase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552:SF147">
        <property type="entry name" value="CHOLINE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552">
        <property type="entry name" value="GLUCOSE-METHANOL-CHOLINE GMC OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05199">
        <property type="entry name" value="GMC_oxred_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00732">
        <property type="entry name" value="GMC_oxred_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000137">
        <property type="entry name" value="Alcohol_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00623">
        <property type="entry name" value="GMC_OXRED_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00624">
        <property type="entry name" value="GMC_OXRED_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 6" id="KW-0274">FAD</keyword>
    <keyword evidence="3 6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucose-methanol-choline oxidoreductase N-terminal" evidence="7">
        <location>
            <begin position="135"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="Glucose-methanol-choline oxidoreductase N-terminal" evidence="8">
        <location>
            <begin position="316"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="278"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000137-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS00623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS00624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003785-PD"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="631" mass="69965" checksum="67ED77671E339C1E" modified="2020-08-12" version="1">MEALMGGQCAAQSVGPANQLFGLLVQTILAAQCAISPPDMWPKDYGPTALQRGLDEYDFVIVGAGSAGSVVANRLSENPDWKVLLLEAGGDPPIESEIASMAMALQHSDVDWAYNVQRSDSSSLGTRNGTFWPRGRTLGGSGAINAMMYLRGNRRDYDRWQSLGNPEWGWEDVLPYFRKSENMNNPTLVRGEGAKYHRTGGYLNVEQRIDNTTLNGILRRGALELGYEWIDDFNRDRHNGYGNTQYTIIGGTRCSPAKAFLTPVRQRQNLHVIKHAFVDRVLIDERNVATGVRFVVDGSQRVQQVAVRREVILAAGAINTPQLLMLSGVGRTDELKQFGIPPKVDLNVGGNLQDHVAVPLFFKFYALQEQDINDQLARINELYTYVVQNRSQAVVRTGPLNTGAFLNTKNTSDPFPNLQILNFAFPRGGGFSEAQARHFEFTDIISASVQEVDRVTPAMYVHITALNPKSRGRVKLSSANPRVHPIIEANYFEHTDDLNVLVQGIRLQQRLLQTEAFRNAGAALHRIDIPGCRELVYDTEAYWKCYVRQLTVTTYHPVGTAKMGPATDPDAVVDSKLRVRGVHGLRVIDASIMPLIVSGNTNAPTIMIAEMGSDFIKQEHGELLDENGNEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VH89</accession>
    <name>A0A6E8VH89_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Annexin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="domain">
        <text evidence="6">A pair of annexin repeats may form one binding site for calcium and phospholipid.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the annexin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040219651.2">
        <property type="nucleotide sequence ID" value="XM_040363717.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VH89"/>
    <dbReference type="EnsemblMetazoa" id="ACON003790-RE">
        <property type="protein sequence ID" value="ACON003790-PE"/>
        <property type="gene ID" value="ACON003790"/>
    </dbReference>
    <dbReference type="GeneID" id="120947902"/>
    <dbReference type="KEGG" id="acoz:120947902"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004607"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003790"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_029479"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012506">
        <property type="term" value="C:vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001786">
        <property type="term" value="F:phosphatidylserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032509">
        <property type="term" value="P:endosome transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000001">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000002">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000004">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000010">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.10">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001464">
        <property type="entry name" value="Annexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018502">
        <property type="entry name" value="Annexin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018252">
        <property type="entry name" value="Annexin_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037104">
        <property type="entry name" value="Annexin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10502">
        <property type="entry name" value="ANNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10502:SF233">
        <property type="entry name" value="ANNEXIN B9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00191">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00196">
        <property type="entry name" value="ANNEXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00335">
        <property type="entry name" value="ANX"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47874">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00223">
        <property type="entry name" value="ANNEXIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51897">
        <property type="entry name" value="ANNEXIN_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0041">Annexin</keyword>
    <keyword evidence="3 6" id="KW-0106">Calcium</keyword>
    <keyword evidence="5 6" id="KW-0111">Calcium/phospholipid-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003790-PE"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="324" mass="35944" checksum="14703C375EDD5245" modified="2020-08-12" version="1">MSSSQYYPFQCTPTVYPADPFDANEDAATLRTAMKGFGTDEKAIIEVLARRGIVQRLEIAQAFKTSFGKDLISDLKSELGGKFEDVILALMTPLPQFYAKELHDAISGIGTDEEAIIEILCTLSNYGIRTIAEFYEQMYNVSLESDLKGDTSGAFKRLCVSLVQGNRDENNGVDEGAAAADAQALFEAGEGQWGTDESVFNQILVTRSYQQLRAVFDIYESLAGHSIEDAIKREFSGAIEEGFKAIVRCVRSKVQYFAKRLHNSMAGLGTNDKTLIRIIVSRSEIDLGDIKEAFQEMYGKSLESWIKDDLSGDYRDVLVTLTSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VIX2</accession>
    <name>A0A6E8VIX2_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glutathione transferase</fullName>
            <ecNumber evidence="3">2.5.1.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">GST class-theta</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GST superfamily. Theta family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="3"/>
    <dbReference type="RefSeq" id="XP_040221733.2">
        <property type="nucleotide sequence ID" value="XM_040365799.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VIX2"/>
    <dbReference type="EnsemblMetazoa" id="ACON004164-RC">
        <property type="protein sequence ID" value="ACON004164-PC"/>
        <property type="gene ID" value="ACON004164"/>
    </dbReference>
    <dbReference type="GeneID" id="120948928"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001564"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004164"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039141"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03177">
        <property type="entry name" value="GST_C_Delta_Epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03045">
        <property type="entry name" value="GST_N_Delta_Epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000034">
        <property type="entry name" value="glutathione S-transferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000007">
        <property type="entry name" value="Glutathione S-transferase 1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43969">
        <property type="entry name" value="GLUTATHIONE S TRANSFERASE D10, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43969:SF9">
        <property type="entry name" value="GLUTATHIONE S TRANSFERASE D10, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01153">
        <property type="entry name" value="Main.4:_Theta-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="7">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="8">
        <location>
            <begin position="86"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00041523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004164-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="209" mass="23797" checksum="F6076EF9C271501D" modified="2020-08-12" version="1">MDFYYLPGSAPCRAVQMTAAAVGVELNLKLTDLMKGEHMKPEFLKINPQHCIPTLVDNGFALWESRAICTYLAEKYGKDDKLYPKDPQKRAVVNQRMYFDMGTLYQRFADYYYPQIFAKQPANPENEQKMKDAVGFLNTFLDGHKYVAGDSLTIADLSILATISTYDVAGFDLAKYQHVAAWYENIRKEAPGAAINQAGIEEFKKYFEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VIX4</accession>
    <name>A0A6E8VIX4_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Elongation of very long chain fatty acids protein</fullName>
            <ecNumber evidence="10">2.3.1.199</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Very-long-chain 3-oxoacyl-CoA synthase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a very-long-chain acyl-CoA + malonyl-CoA + H(+) = a very-long-chain 3-oxoacyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:32727"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:90725"/>
            <dbReference type="ChEBI" id="CHEBI:90736"/>
            <dbReference type="EC" id="2.3.1.199"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the ELO family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.199" evidence="10"/>
    <dbReference type="RefSeq" id="XP_040226788.1">
        <property type="nucleotide sequence ID" value="XM_040370854.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VIX4"/>
    <dbReference type="EnsemblMetazoa" id="ACON004373-RA">
        <property type="protein sequence ID" value="ACON004373-PA"/>
        <property type="gene ID" value="ACON004373"/>
    </dbReference>
    <dbReference type="GeneID" id="120951872"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005842"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004373"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033942"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009922">
        <property type="term" value="F:fatty acid elongase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034625">
        <property type="term" value="P:fatty acid elongation, monounsaturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034626">
        <property type="term" value="P:fatty acid elongation, polyunsaturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019367">
        <property type="term" value="P:fatty acid elongation, saturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002076">
        <property type="entry name" value="ELO_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157:SF167">
        <property type="entry name" value="ELONGATION OF VERY LONG CHAIN FATTY ACIDS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157">
        <property type="entry name" value="FATTY ACID ACYL TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01151">
        <property type="entry name" value="ELO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9 10" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="5 10" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2 10" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7 10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="26"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="66"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="170"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="204"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="234"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="259"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004373-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="336" mass="39352" checksum="476AC23B6D8B274E" modified="2023-06-28" version="2">MALIMKYIDSMHHYMDKYGDPRTKEWPLMSSPLPTLALCLGYVYLVKVLGPRLMENRKPFQLKNTLIVYNFVQVVFSAWLFYECLMGGWWGEYSFRCQPVDHGTTGRAMRMVHACWWYYFSKFTEFFDTFFFVMRKKSSQVSTLHVIHHGCMPMSVWFGVKFTPGGHSTFFGLLNTFVHIVMYTYYLFTALGPQFQKYLWWKKYLTALQMVQFVLIMVHAFQLLFIDCNYPKAFVWWIGMHAVMFFFLFNEFYQNTYKVQKQKRAAAAKKREAEALLLQQQQQQQQQNGHVANGSANGTLSNGTGNKAADYYVRGDLPAEMELTQRPATNTHTAAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VIX5</accession>
    <name>A0A6E8VIX5_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glutamate dehydrogenase [NAD(P)(+)]</fullName>
            <ecNumber evidence="3">1.4.1.3</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-glutamate + NAD(+) + H2O = 2-oxoglutarate + NH4(+) + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15133"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.4.1.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-glutamate + NADP(+) + H2O = 2-oxoglutarate + NH4(+) + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11612"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.4.1.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Glu/Leu/Phe/Val dehydrogenases family.</text>
    </comment>
    <dbReference type="EC" id="1.4.1.3" evidence="3"/>
    <dbReference type="RefSeq" id="XP_049462669.1">
        <property type="nucleotide sequence ID" value="XM_049606712.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VIX5"/>
    <dbReference type="EnsemblMetazoa" id="ACON004362-RE">
        <property type="protein sequence ID" value="ACON004362-PE"/>
        <property type="gene ID" value="ACON004362"/>
    </dbReference>
    <dbReference type="GeneID" id="120961082"/>
    <dbReference type="CTD" id="42832"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004833"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004362"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040704"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004352">
        <property type="term" value="F:glutamate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006538">
        <property type="term" value="P:L-glutamate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01076">
        <property type="entry name" value="NAD_bind_1_Glu_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10860:FF:000007">
        <property type="entry name" value="Glutamate dehydrogenase 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000100">
        <property type="entry name" value="Glutamate dehydrogenase 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006095">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006096">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006097">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033524">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033922">
        <property type="entry name" value="NAD_bind_Glu_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11606">
        <property type="entry name" value="GLUTAMATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11606:SF13">
        <property type="entry name" value="GLUTAMATE DEHYDROGENASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00208">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02812">
        <property type="entry name" value="ELFV_dehydrog_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00082">
        <property type="entry name" value="GLFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00839">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00074">
        <property type="entry name" value="GLFV_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamate/phenylalanine/leucine/valine/L-tryptophan dehydrogenase C-terminal" evidence="9">
        <location>
            <begin position="261"/>
            <end position="567"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004362-PE"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="570" mass="63168" checksum="F2A998890999F63C" modified="2020-08-12" version="1">MYHLKTIAKNATIKQEVATLVKAVAAVQQPMAMNQQQPKRTYAEHQIPERLKDVSTAANPRFFDMVEYFFHRACQICEDKLVEDMKGRASMDEKKKKVKGILMLMQPCDHIIEIAFPLRRDSGDYEMITGYRAQHCTHRTPTKGGIRFSLDVSRDEVKALSALMTFKCACVDVPFGGAKAGVKIDPRKYSEHELEKITRRFALELSKKGFIGPGIDVPAPDMGTGEREMSWIADTYAKTIGHLDINAHACVTGKPINQGGIHGRVSATGRGVFHGLDNFIKEANYMAQIGTTPGWGGKTFIVQGFGNVGLHSCRYLTRAGATCIGVIEHDGSIFNPQGIDPKALEDYKNEKGTIVGFPGAQPYEGENLMYEQCDIFIPAAVEKVITAENAGKINAKIIAEAANGPTTPAADKILIDRNILVIPDLYINAGGVTVSFFEWLKNLNHVSYGRLTFKYERESNYHLLASIQQSLERFVFSDEEKSVQASLERRFGNVGGKIPVTPSEAFQKRISGASEKDIVHSGLDYTMERSARAIMKTAMKYNLGLDLRSAAYVNSIEKIFQTYRDAGLAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VIX6</accession>
    <name>A0A6E8VIX6_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">DNA polymerase alpha subunit B</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Accessory subunit of the DNA polymerase alpha complex (also known as the alpha DNA polymerase-primase complex) which plays an essential role in the initiation of DNA synthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the DNA polymerase alpha subunit B family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040225793.2">
        <property type="nucleotide sequence ID" value="XM_040369859.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VIX6"/>
    <dbReference type="EnsemblMetazoa" id="ACON004392-RB">
        <property type="protein sequence ID" value="ACON004392-PB"/>
        <property type="gene ID" value="ACON004392"/>
    </dbReference>
    <dbReference type="GeneID" id="120951261"/>
    <dbReference type="CTD" id="136028709"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004350"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004392"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042258"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005658">
        <property type="term" value="C:alpha DNA polymerase:primase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007185">
        <property type="entry name" value="DNA_pol_a/d/e_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016722">
        <property type="entry name" value="DNA_pol_alpha_bsu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23061">
        <property type="entry name" value="DNA POLYMERASE 2 ALPHA 70 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23061:SF12">
        <property type="entry name" value="DNA POLYMERASE ALPHA SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04042">
        <property type="entry name" value="DNA_pol_E_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018300">
        <property type="entry name" value="DNA_pol_alph_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA polymerase alpha/delta/epsilon subunit B" evidence="8">
        <location>
            <begin position="346"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="166"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="245"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="19"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="40"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="166"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="199"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="245"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="258"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR018300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004392-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="613" mass="67338" checksum="135FFC09107708BC" modified="2020-08-12" version="1">MKKRSGGGEAVETAKKTVSRLQHNVNTPNGVRGGGSGGSPFTTVTYSPLSASSKKNDPDAPSKAGQIVYTYGSAKLLKQFNWLPTTTDEGKEPITIDDDVSLGDRNRRTGAGRGKGIGFTIKSAQFANDGCKYMYDSSYDRVMILGDRIYECGNAICKRVALEAVKPKSHEQPAKNNDEPDVKSNSALDTPLPVKEEPASEETDVDIEREEDRLSSWFEGIKVHHVDYPSSELIRVLGRIVVESSSTTTNSSSGTTSDKQVASNRNPTTTSKLSIVDFDEHTLRYTRLDLSKVQVPSWWSLFPGQTVLLEGINPRGTLFSVKQIHCERTLALPRVPSSLDKTLTCVIASAPFTDRDDLLYEKLSSLLVYCSNNPPDVLILTGPFADANCKLYAEVAETFDEYFEKIISTIMSSIGIYTEVFVVSHHDDLVSSFVYPTPPYKVASFYKNLHFLPDPCVFNLEGVEIGITTVDVIKHLIESECTAANVTIGSAPPIGGDKIKRAYGHLFHQASFYPLNPPPEDVPLDVDMLNEFGRLNRVPNIMICPSALNRYVREINGCVCINPGFVDGHSADGSYARLIIQAPEIDEQQQRESQSDSIQPSPASYIACQIVKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VIY3</accession>
    <name>A0A6E8VIY3_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="9">MFS domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_040220702.1">
        <property type="nucleotide sequence ID" value="XM_040364768.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VIY3"/>
    <dbReference type="EnsemblMetazoa" id="ACON004402-RA">
        <property type="protein sequence ID" value="ACON004402-PA"/>
        <property type="gene ID" value="ACON004402"/>
    </dbReference>
    <dbReference type="GeneID" id="120948444"/>
    <dbReference type="CTD" id="3354861"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_003173"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004402"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035826"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006820">
        <property type="term" value="P:monoatomic anion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17318">
        <property type="entry name" value="MFS_SLC17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000003">
        <property type="entry name" value="Solute carrier family 17 member 3"/>
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    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050382">
        <property type="entry name" value="MFS_Na/Anion_cotransporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11662:SF77">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY TRANSPORTER 17, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11662">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0769">Symport</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="18"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="114"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="145"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="173"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="202"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
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            <begin position="227"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
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            <end position="310"/>
        </location>
    </feature>
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            <end position="354"/>
        </location>
    </feature>
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            <end position="388"/>
        </location>
    </feature>
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            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
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            <end position="449"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="455"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="19"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004402-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="529" mass="59017" checksum="CFEFC9B19D596132" modified="2020-08-12" version="1">MESAHVHVRDKDRIPARLVLYFLSWSGFLVSFVMRNDINFALVAMVQDPSANTTGSDHCNTLVNDMPSLEEGDNYNYTLSVANATTTVLLDGPASIDDIKFDWDSTVQAVIKSSFYWCYVLSQVVGGVATQYFGTKNVFGWSQFLTAACSLLIPHAADLHYGAVILLRSVQGFASGLTWPAMYAIVGYWIPPVERSRFMSSFQGFSIGIGLTYPLCGFIIAHFGWRQVFYTTGTIGMVWCVFWYLLAYNTPQEHPRITPEELEYIELNVSEDIKNGQGMRVPWRRIFTSMPVWAIGLTTFGRIWVHYTFIMSGPEYMQKIFCFDIQQNGLLSGAPFLCSYLSSVLFCYVADLLMDNKTLTLTNVRKLFTALSQIVPGVLVLLVGYLGYQIVTVLILWFIAVTFITASYAGAMANIVDIAPNLAGPVLAFAQTIHMTASFLQPLVTGFMVTDSQNINQWLHVFGVSSVVAISTYLIYQVFGTAEIQSWNYPVPDPEVTSSDDSAVIANQPMIKVEPGLKFDDDDDEDDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VIY6</accession>
    <name>A0A6E8VIY6_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VIY6"/>
    <dbReference type="EnsemblMetazoa" id="ACON004179-RA">
        <property type="protein sequence ID" value="ACON004179-PA"/>
        <property type="gene ID" value="ACON004179"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_003960"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004179"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042095"/>
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        <property type="component" value="Unplaced"/>
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    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:calcium ion binding"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:calcium import into the mitochondrion"/>
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        <property type="term" value="P:mitochondrial calcium ion homeostasis"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="EFh_MICU"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="EF-hand-dom_pair"/>
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        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
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        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
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        <property type="entry name" value="MICU1/2/3"/>
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        <property type="entry name" value="EF HAND DOMAIN FAMILY A1,A2-RELATED"/>
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    </dbReference>
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        <property type="entry name" value="MITOCHONDRIAL CALCIUM UPTAKE 3, ISOFORM D"/>
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    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="171"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="395"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004179-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="433" mass="50615" checksum="08B161FF97CFF883" modified="2020-08-12" version="1">MAMYTNRVVRCSKTNPFFTVLSRNFGNNSSARKWYQHPTVRYICLVSGGLLAFTAATYRNRPKVYALQLRKDEIADKAIKLTARERRFIKFASVEFDGQIYMTPQDFLESVVEQEPRPRLKRKSLTHAEIQQLKDYTPPLKHGSSQLFRSLRDKGIISYTEYLFLLSVLTKPHSGFRIAFNMFDTDGNQRVDKDEFLVIRQLLGGSLKDRDLDEATRQAMEKIFSFAWKGKRGIESKEGEEPGEHDDYVDDEQGLQRKHKVDTTLQIHFFGKKGDKDLQYDGFYNFMKNLQTEVLELEFCEFSKGHERISEVDFAKILLRYTYLDTDEYDNYLDRLLDREAKEKGVSFDEFRQFCQFLNNLDDFSIAMRMYTLADQPISKDEFSRAVKICTGSELNSHLIDTVFAIFDEDGDGLLSYKEFIAIMKDRVHRGFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VJ93</accession>
    <name>A0A6E8VJ93_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">AP complex subunit sigma</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">Ngousso</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="11">Adaptor protein complex 1 (AP-1) is a heterotetramer composed of two large adaptins (gamma-type subunit AP1G1 and beta-type subunit AP1B1), a medium adaptin (mu-type subunit AP1M1 or AP1M2) and a small adaptin (sigma-type subunit AP1S1 or AP1S2 or AP1S3).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <location evidence="3">Clathrin-coated pit</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the adaptor complexes small subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_049462839.1">
        <property type="nucleotide sequence ID" value="XM_049606882.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VJ93"/>
    <dbReference type="EnsemblMetazoa" id="ACON004283-RA">
        <property type="protein sequence ID" value="ACON004283-PA"/>
        <property type="gene ID" value="ACON004283"/>
    </dbReference>
    <dbReference type="GeneID" id="120947936"/>
    <dbReference type="CTD" id="42835"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002167"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004283"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_036938"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030121">
        <property type="term" value="C:AP-1 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035615">
        <property type="term" value="F:clathrin adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd14831">
        <property type="entry name" value="AP1_sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.60:FF:000005">
        <property type="entry name" value="AP complex subunit sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044733">
        <property type="entry name" value="AP1_sigma"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016635">
        <property type="entry name" value="AP_complex_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022775">
        <property type="entry name" value="AP_mu_sigma_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000804">
        <property type="entry name" value="Clathrin_sm-chain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11753">
        <property type="entry name" value="ADAPTOR COMPLEXES SMALL SUBUNIT FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01217">
        <property type="entry name" value="Clat_adaptor_s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015588">
        <property type="entry name" value="AP_complex_sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00989">
        <property type="entry name" value="CLAT_ADAPTOR_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0168">Coated pit</keyword>
    <keyword evidence="10" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AP complex mu/sigma subunit" evidence="13">
        <location>
            <begin position="1"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00065677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR015588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004283-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="157" mass="18311" checksum="C5CC46B8004AA8F1" modified="2020-08-12" version="1">MLQFMLLFSRQGKLRLQKWYVAHSDKVKKKITRELITTILSRKPKMCSFLEWKDCTIVYKRYASLYFCCAIEQNDNELLTLEVIHRYVELLDKYFGSVCELDIIFNFEKAYFILDELLVGGEIQETSKKNVLKAIAAQDVLQEDEAADGALRDIGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VKZ6</accession>
    <name>A0A6E8VKZ6_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DUF4806 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_040224956.2">
        <property type="nucleotide sequence ID" value="XM_040369022.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VKZ6"/>
    <dbReference type="EnsemblMetazoa" id="ACON005106-RB">
        <property type="protein sequence ID" value="ACON005106-PB"/>
        <property type="gene ID" value="ACON005106"/>
    </dbReference>
    <dbReference type="GeneID" id="120950751"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_013125"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005106"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041272"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032071">
        <property type="entry name" value="DUF4806"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16064">
        <property type="entry name" value="DUF4806"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4806" evidence="2">
        <location>
            <begin position="344"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005106-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="483" mass="54222" checksum="C9933B734A64D36B" modified="2020-08-12" version="1">MENSQTKRSTRSAAPPAVSAEMPELKEEPIDDDISLDHTRQAATESTHQEGFVSSGARDPFLDEMEIGETSITFPNAFLDAFGQDVKTEHPTAEESDLNAEAVEAAGSSTAKRKNCDDEESEEDSSSATKRLPNSNSSIIRCKIVQTRDAKGHVKLSVVPDHRVSVPSNPKKIQLNCTDANEQTQNHKHSIYQEVDRSKQEHQRITSEDADSLITMSDESLSGFSVNNSMIVHTLQDDNSDIEDPTSKQTTTPVSRVAASNIGISESKMIRKARCSNCKTMTAMLKAQTKLLINIRSQQKRTTNEMLKNQKILMNRMAAMELRIKTLLNDENADEANKNADESIPFFDQPLVASLEGLEKLDKDLEDEGYFRNLTNELLIQIVDEDPNNRMLTVLDAIFDKTFLTTCSWTGISKKCRKIAMISYQNVLKVFQSVGSTHEDKVTKDMVQSFFMNKLKHAKERLKAKGLVRSKCRTRQTFTLYKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VKZ7</accession>
    <name>A0A6E8VKZ7_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Calnexin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Calcium-binding protein that interacts with newly synthesized monoglucosylated glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Required for embryogenesis and larval development under heat and ER stress conditions. May be important for germ cell development. Involved in neuronal necrotic cell death.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the calreticulin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040229763.1">
        <property type="nucleotide sequence ID" value="XM_040373829.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VKZ7"/>
    <dbReference type="EnsemblMetazoa" id="ACON005032-RA">
        <property type="protein sequence ID" value="ACON005032-PA"/>
        <property type="gene ID" value="ACON005032"/>
    </dbReference>
    <dbReference type="GeneID" id="120953673"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000884"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005032"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033959"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.250.10:FF:000001">
        <property type="entry name" value="Calnexin homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000011">
        <property type="entry name" value="Probable calnexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.250.10">
        <property type="entry name" value="Calreticulin/calnexin, P domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001580">
        <property type="entry name" value="Calret/calnex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018124">
        <property type="entry name" value="Calret/calnex_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009033">
        <property type="entry name" value="Calreticulin/calnexin_P_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11073:SF1">
        <property type="entry name" value="CALNEXIN 14D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11073">
        <property type="entry name" value="CALRETICULIN AND CALNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00262">
        <property type="entry name" value="Calreticulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00626">
        <property type="entry name" value="CALRETICULIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63887">
        <property type="entry name" value="P-domain of calnexin/calreticulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00804">
        <property type="entry name" value="CALRETICULIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00805">
        <property type="entry name" value="CALRETICULIN_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0143">Chaperone</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026379364" description="Calnexin" evidence="10">
        <location>
            <begin position="33"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="482"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="263"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="517"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="280"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="553"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="572"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="159"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00053392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR601580-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005032-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="632" mass="71054" checksum="49417CF15B2269A0" modified="2020-08-12" version="1">MMKLNRPRWSPLLWRIALLSAVLLFHNPVVHGNDEISTESQPAGDAEQYETPTVDPKRMYFAEHFDDLFDGASDAIEKRWVKSKAKKDDAAEEVAKYDGEWAVEQPQRPILANDYGLVLKSKAKHAAIASPLLLNRPFVFEDKPLVVQYEVNLQEGQECGGSYIKLLSVGDGTKDLKQFHDKTPYTIMFGPDKCGNDVKLHFIFRHVNPINGTITEKHCNKQKDRLDDPFKDKRPHLYQLIIRPDNTYTIRVDHKTVNEGSLLTNFTPAVNPPAEIDDPEDRKPENWDDREKIPDPEATKPDDWNEDEPAQIPDPKATKPAGWLDDVEEMVPDSSAVKPEDWDNDMDGEWEPPLIPNPMCEKAVGCGLWKAPMVPNPNYKGKWRPPLIANVNYQGKWAPRKIPNPDFFEDLTPFRMTPIAAVGIELWSMSNDILFDNIVICDDESVADEWAAQTFDLKVKTLDRQAETFVSRVLKYCNSNPWMWAVLIVVVGLPVALMIYFMCFTKGDAKAEIGRKKKNDDYPAPEQEQDELVGGAGEEAEDDGRSSKTKLNAAATGSSGSRSASSAGEAYAENEDERDGDEAEEDEEDDEAQEEAQEMEAGEEEASSSDAPVDSKNSEGGEGLKKRRARKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VNA2</accession>
    <name>A0A6E8VNA2_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="EnsemblMetazoa" id="ACON005803-RA">
        <property type="protein sequence ID" value="ACON005803-PA"/>
        <property type="gene ID" value="ACON005803"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000862"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005803"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030900"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="91"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="159"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="245"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="351"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="604"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="690"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="802"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="923"/>
            <end position="977"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1072"/>
            <end position="1093"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1277"/>
            <end position="1545"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1565"/>
            <end position="1625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="167"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="193"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="249"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="351"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="374"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="412"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="621"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="690"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="804"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="817"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="851"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="923"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1079"/>
            <end position="1088"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1289"/>
            <end position="1302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1322"/>
            <end position="1332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1391"/>
            <end position="1407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1415"/>
            <end position="1445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1460"/>
            <end position="1481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1496"/>
            <end position="1510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1516"/>
            <end position="1542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1574"/>
            <end position="1587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1603"/>
            <end position="1625"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005803-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1625" mass="170377" checksum="1DD4060D5454F196" modified="2020-08-12" version="1">MGKCASKQPAPGLNEFGSAPYLAICSEDDILYINVGQSESKKASKKAATDTGLHTKGTAMSFGFKKHKVHQATTGSNGTIGTTGNNKLPPGAVTHTGDGGGGVGAGAEPVPGAVANQIKKFSHSNHNNNNNNNNNNSNNATLEREKAERELVQATVISNPGVDAAFSDNNGNSGSVESVDDTSGGVMGPGRTAASTGRSTPRLQPAKKDSNGAPYRSNRFGFRTNNIVRPASVGLQPRVANDFDKHSSTTTHTNNNNNNIHAHNNNNNNVYVVNDKRRSKSASSAASARTTFTPLPGTTAAAAAATGTGGNGAIVLHQHAYGGGATALHRPLPKYQAPSTKIAHATVPDSNSGAGGTYQQQMTKCNQGEHKNTQHSGAGTKQTAQQHQYTHGSEAGQNAPSGGGLCAKPPLTGNGHTVGGTTTSAKLGQETAATASKFTLHTSSLPKPQYPVSISLTGTTAGSAAAAATATASSRYTMDTKGAKHAVNVSRKEFANTNVTGAPALEDTTTTTTSSIRMPRQRYRNLEMVMSGRHKFEVRDLEALTTEPIVPLPLPELPSAFCSTGNQRTAPLSGLIRSTELNASANASALHDDIDINDNRYEQAEPGAAEEQVTPTAPDGEGQKESLEEEKLLRDNNSSEKSLIKGGAMKELMESYEESKSSTPSCSRASWFTAGEALAAKDFGIRSLTSSVESSTSRQTDSMPGSAQDEQEEEDISSVTITAGNPSAFSSLSAPIPMDVSATNLDSVSEVLVSFTDPGTDPLASGGGDAATEPNRMYRNEQAKFAEMAAAISDVLLLDDETSPTDSLVSSCTENSDDVKKPRGGKKTAENTKEKEKDIDEISPELDELTSPVSPGTPTHASNSLSLSDGGRDFLIDDEIADQPGLVFDEGSTLDLGSLNLNLTSQKTDTDRTLKDNHNRAAAAAAGTTNGAGSQPAAIRATPPSAPKPRRAMPDAYESPALSRKSARSGNRMARAESLDTLSPCDSIASDDFMLDFDCNSSMDSIDRVARSSMGGSANGLNSMDELQLWSELENKGGHIIREWSTLLRSSPQSNSTHRSQLPARARLLTRRLQNNATPTNGSESPRSIDSLPRRTFAGSYKTATLSQFHNLANNNNNCTDSASTATNSAEDLTLLDKSLRNSMLQDVVHFKKQLVRLRRIMQEDEENLMMTDTLNPFENNNGQFFTTAAAAMAANGTIGSATTTTATTATSQEQQQQQQQENILIRESSVAALALLEDQRQELADLRRQVVYLQGELTAKDRTIRQQQNLIEKYEAEREKQQQQQQQLHSLTNGGSSTESGDGTALSGPDSAGSSDRNHQSAETISTATQTERLRPVSFGGQEGLGSRSEKPVTPKNGLRTPSAVGLAGATPPHQHHHHHHTNGTPTTPKSKTQISSVYTQLSSVRHSYAGNGSAPTTPTHNAGAQNGLRRTSLGSSHNLSTFGLHSPSERSPHGSNKPVRTTQIGTLASPIQRQPPNGGTTVKPPPSKAVPPSRTNGVVTASKLNGLNGTAKRPAAGTTTGSSSIIRPPSSFGSSSSLASVGEPLKSKSAPLVVMPNGKLLTPAATGAATSDGREPSGSESDKDTVVVNGGAIGTAGSCCSEENNLASAGNTNGSTVNGIVGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VNA3</accession>
    <name>A0A6E8VNA3_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ULP_PROTEASE domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C48 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VNA3"/>
    <dbReference type="EnsemblMetazoa" id="ACON005881-RB">
        <property type="protein sequence ID" value="ACON005881-PB"/>
        <property type="gene ID" value="ACON005881"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_003903"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005881"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031904"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070139">
        <property type="term" value="F:SUMO-specific endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016926">
        <property type="term" value="P:protein desumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.130:FF:000007">
        <property type="entry name" value="polyhomeotic-proximal chromatin protein isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.20:FF:000001">
        <property type="entry name" value="sentrin-specific protease 6 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.130:FF:000005">
        <property type="entry name" value="Uncharacterized protein, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.395.10">
        <property type="entry name" value="Adenoviral Proteinase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003653">
        <property type="entry name" value="Peptidase_C48_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051947">
        <property type="entry name" value="Sentrin-specific_protease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46896:SF3">
        <property type="entry name" value="FI06413P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46896">
        <property type="entry name" value="SENTRIN-SPECIFIC PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02902">
        <property type="entry name" value="Peptidase_C48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50600">
        <property type="entry name" value="ULP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin-like protease family profile" evidence="7">
        <location>
            <begin position="212"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="337"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="384"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="588"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="635"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="349"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="386"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="635"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="649"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="721"/>
            <end position="734"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005881-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="734" mass="80956" checksum="221ED5A62040CDAC" modified="2020-08-12" version="1">MVDRYKPRLDITDVKNLPDGQITQIDCSTIRVGTLKYEANEKVTISSKGVRIVAPNVKRPTEFVSLDLQMNEMKKVLTHFSKGLNVIFLYTNISGGAYVRKHLEMDPSSDKLPCFRPEIASNDLTKRIILLVDSISEEAKSIIKNIFHPDLLEEISIRDAKELILRCSSSRSSNTSSAANALVDANGSGSNDTNDSLEIRTILIYPPGPGGITINTEDYLCLAKDQYLNDIIIDFYLNYLKLEMLDDGERQSVHIFSTFFYNRLTTLTTRQRGPPGDRGDVRLSAAQKRHARVANWTKKDNIFDKKFIVIPINEQSHWFLAIICFPGLDCPVTASNNTPAPSVSTVAKPKPKSAPRSKRNLTMQIGSTTITPVSKREIESIHLADDDMCERDEADGDESELATDAEDSDDEPSDDGRPPIKQPCILIFDSLTGASRSRVVATLRDYLTCEYRVKMPDKPAKQFNKLNMPGHCVKVPQQNNYTDCGLYLLQYVEHFFLDPILDYHLPIKQLQDWFETITVTKKREDISNLIKELIDKHDPSAPPLPSIELPTLNGKLIIDPDDSLNDAEFEEEEMEHDEEFVSAFDMTPDTDEQLSSPAKGTDGKAAETIITSSPPVKPIGSKKIINFKRLGGQSLINRSSSSGNNLQDGGTSSTEGGTTKAATSSSSSTSTTTSSSSNSSSSNNNSNSGTGNTKRLLDGNGSKLAGSQQDGSVRQKIPRLSNDSSASKVVTTQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VNA7</accession>
    <name>A0A6E8VNA7_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Zasp-like motif domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VNA7"/>
    <dbReference type="EnsemblMetazoa" id="ACON005807-RA">
        <property type="protein sequence ID" value="ACON005807-PA"/>
        <property type="gene ID" value="ACON005807"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010946"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005807"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035036"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="29"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="70"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="143"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="157"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="171"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="191"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="233"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005807-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="297" mass="34466" checksum="5D5C7B91ED1A16FD" modified="2020-08-12" version="1">MMAYQQRVSQYRSPRRIDFDDPSTTKPPSLAKSAVLAALEEEEREKSRGTGLKRVAWPPPADGPEYEVHPSQQKQQPVGQQIPLQQQQQQQQFQPQQFYHQTNPQQQQQQQQPAYQQTSFPPQQQHPHQPVERIVPIQRPEAQHQQQPLAPQYQNPLEQQPYQPQPTEAFQPLTAQQQQPLYQPAQPPQPHQQLPPTLITTLRKEPPMSQEPAPVYQTQPVAAIYQGGSNMRGDQKWPPEEYKRQSEVDNEERRKLAQQPAFRPRRQQKDYADFFAKNALNNTYPGYRAPPGTQHHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VNA9</accession>
    <name>A0A6E8VNA9_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VNA9"/>
    <dbReference type="EnsemblMetazoa" id="ACON005762-RB">
        <property type="protein sequence ID" value="ACON005762-PB"/>
        <property type="gene ID" value="ACON005762"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001674"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005762"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032472"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="136"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="210"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="240"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="259"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005762-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="295" mass="33019" checksum="8872899F8B0F9C21" modified="2020-08-12" version="1">MMMMIKQQKARKQEPIQRVRVVLRDLHENNLDVASIVTTAATGSAMAAMVKANEPPTVEGQHQLDQQLSNEPCPSTSTGKGGQQQAGTPQIVVGTIELNNLNGDIANHKEKEIGTSNQFETLTLIDEDDEEEQDRNCSNAHSSSSLLSSNNDNRSAEENNLHNASTLAIGEQHRRRSRRRSSQKDRRSAEDIDSIDQADSAVDVRHHDEEEPNVFDDEVREEDQKQQKAGSSPNASAAAVRRRKNQRQRSRQRRSPALAHRDQVDGVDGEEQKEPYEKAVTCLYWSLACWDCNIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VNB4</accession>
    <name>A0A6E8VNB4_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_040228175.2">
        <property type="nucleotide sequence ID" value="XM_040372241.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VNB4"/>
    <dbReference type="EnsemblMetazoa" id="ACON005890-RB">
        <property type="protein sequence ID" value="ACON005890-PB"/>
        <property type="gene ID" value="ACON005890"/>
    </dbReference>
    <dbReference type="GeneID" id="120952758"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007227"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005890"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041659"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026316830" evidence="2">
        <location>
            <begin position="18"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="40"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005890-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="135" mass="14609" checksum="EDFA801BDE96462F" modified="2020-08-12" version="1">MFKFVLISALLVAAVCAAPADNSKPGQQGRTDGLDQAESAWNNNPNAWNAGQNTWGRDQAAWNHPSSWNHGSAWNHPNAWNRGYNNRYDPANRWGAGADQWNRYGAAGAGYNSWAGRGAAAGWPAGAAGNRWNAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VNC0</accession>
    <name>A0A6E8VNC0_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="13">V-SNARE coiled-coil homology domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Secretory vesicle</location>
            <location evidence="1">Synaptic vesicle membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the synaptobrevin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VNC0"/>
    <dbReference type="EnsemblMetazoa" id="ACON005507-RC">
        <property type="protein sequence ID" value="ACON005507-PC"/>
        <property type="gene ID" value="ACON005507"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004804"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005507"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039337"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd15870">
        <property type="entry name" value="R-SNARE_VAMP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000013">
        <property type="entry name" value="Vesicle-associated membrane protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001388">
        <property type="entry name" value="Synaptobrevin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016444">
        <property type="entry name" value="Synaptobrevin/VAMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042855">
        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45701">
        <property type="entry name" value="SYNAPTOBREVIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00957">
        <property type="entry name" value="Synaptobrevin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00219">
        <property type="entry name" value="SYNAPTOBREVN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00417">
        <property type="entry name" value="SYNAPTOBREVIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50892">
        <property type="entry name" value="V_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0770">Synapse</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="V-SNARE coiled-coil homology" evidence="12">
        <location>
            <begin position="44"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="73"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="19"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="35"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005507-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="127" mass="13635" checksum="822451D4F1718E6C" modified="2020-08-12" version="1">MSGEQQAESAPDGLAPGAAAGGEPGADGIVGGPRTPQQIAAQKRLQQTQAQVDEVVDIMKTNVEKVLERDQKLSELDDRADALQQGASQFEQQAGKLKRKFWLQNLKMMIIMGVIGLVILAIIVARN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VND6</accession>
    <name>A0A6E8VND6_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Thyroglobulin type-1 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VND6"/>
    <dbReference type="EnsemblMetazoa" id="ACON005942-RA">
        <property type="protein sequence ID" value="ACON005942-PA"/>
        <property type="gene ID" value="ACON005942"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009902"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005942"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030971"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00191">
        <property type="entry name" value="TY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.800.10">
        <property type="entry name" value="Thyroglobulin type-1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051950">
        <property type="entry name" value="Dev_reg/Prot_inhib"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000716">
        <property type="entry name" value="Thyroglobulin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036857">
        <property type="entry name" value="Thyroglobulin_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12352:SF3">
        <property type="entry name" value="NIDOGEN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12352">
        <property type="entry name" value="SECRETED MODULAR CALCIUM-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00086">
        <property type="entry name" value="Thyroglobulin_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00211">
        <property type="entry name" value="TY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57610">
        <property type="entry name" value="Thyroglobulin type-1 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51162">
        <property type="entry name" value="THYROGLOBULIN_1_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026285663" evidence="6">
        <location>
            <begin position="29"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="Thyroglobulin type-1" evidence="7">
        <location>
            <begin position="114"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="Thyroglobulin type-1" evidence="7">
        <location>
            <begin position="179"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="179"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005942-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="314" mass="34553" checksum="6EC15ED336114BA9" modified="2020-08-12" version="1">MMTPARIKSWTFQAFVATLVLIGPIISAQKVPNCASTLGCFSFPAITSCPSPDEFWSPTAVLGGCCPGCVRGLALGTPCGTSTTTETTTDATETPATCAPGLVCGVGSVCRLNTGDCLATHHVPDSIQWVPQCDSRGRYAPKQCRGDQINGRCFCYSAEGKRIFGWSWRNRAQRMTCACSRRRADLEAEGRFDVTLHCTPDGNYEQLQCDQGVCWCADSETGTQTAGTRAVPEELWSLLPCYNETLHGVQYLRRCESVAFSQILQRKQFIIRGHRDVTFTDTLCEYDGSHELHARGMTGRSLILIKLRSEHCRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VPC2</accession>
    <name>A0A6E8VPC2_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Calcium-transporting ATPase</fullName>
            <ecNumber evidence="21">7.2.2.10</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="23">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="21">Catalyzes the hydrolysis of ATP coupled with the transport of calcium.</text>
    </comment>
    <comment type="function">
        <text evidence="20">This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19 21">
            <text>Ca(2+)(in) + ATP + H2O = Ca(2+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18105"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.10"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="21">Membrane</location>
            <topology evidence="21">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Sarcoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18 21">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.10" evidence="21"/>
    <dbReference type="RefSeq" id="XP_049462336.1">
        <property type="nucleotide sequence ID" value="XM_049606379.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_049462337.1">
        <property type="nucleotide sequence ID" value="XM_049606380.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_049462338.1">
        <property type="nucleotide sequence ID" value="XM_049606381.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VPC2"/>
    <dbReference type="EnsemblMetazoa" id="ACON006186-RB">
        <property type="protein sequence ID" value="ACON006186-PB"/>
        <property type="gene ID" value="ACON006186"/>
    </dbReference>
    <dbReference type="GeneID" id="120951795"/>
    <dbReference type="CTD" id="49297"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000733"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON006186"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041260"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033017">
        <property type="term" value="C:sarcoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005388">
        <property type="term" value="F:P-type calcium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd02083">
        <property type="entry name" value="P-type_ATPase_SERCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000143">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000003">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000005">
        <property type="entry name" value="Calcium-transporting ATPase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000065">
        <property type="entry name" value="Sarcoplasmic/endoplasmic reticulum calcium ATPase 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005782">
        <property type="entry name" value="P-type_ATPase_IIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01116">
        <property type="entry name" value="ATPase-IIA1_Ca"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42861">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08282">
        <property type="entry name" value="Hydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 21" id="KW-0106">Calcium</keyword>
    <keyword evidence="5 21" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="16 21" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="17 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0703">Sarcoplasmic reticulum</keyword>
    <keyword evidence="14" id="KW-1278">Translocase</keyword>
    <keyword evidence="6 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 21" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="60"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="90"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="259"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="297"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="759"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="832"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="927"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="22">
        <location>
            <begin position="3"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00038148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00054278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU361146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON006186-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="998" mass="109603" checksum="CA1A2DEE54E434C2" modified="2020-08-12" version="1">MEDGHSKTVDEVLSHFRVDPERGLSLDQVKEYQKKYGPNELPAEEGKTLWQLVLEQFDDLLVKILLLAAIISFVLALFEEHEGVEAFVEPFVILLILIANAVVGVWQERNAESAIEALKEYEPEMGKVIRGDKSGVQKIRAKEIVPGDVVEVSVGDKIPADIRLIKIYSTTIRIDQSILTGESVSVIKHTDAVPDPRAVNQDKKNILFSGTNVAAGKARGVVIGTGLNTAIGKIRTEMSETEEIKTPLQQKLDEFGEQLSKVISLICVAVWAINIGHFNDPAHGGSWIKGAVYYFKIAVALAVAAIPEGLPAVITTCLALGTRRMAKKNAIVRSLPSVETLGCTSVICSDKTGTLTTNQMSVSRMFIFEKIEGNDSSFTEFEISGSTYEPIGEVTLNGQRIKAADYETLHELGTICIMCNDSAIDFNETKKVFEKVGEATETALIVLAEKLNPFNVAKQGLDRRSSAICVRQEIETKWKKEFTLEFSRDRKSMSSYCTPLKASKLGNGPKLFCKGAPEGVLERCTHARVGSTKVPLTQTLKQRILDLTRTYGTGRDTLRCLALATADSPMKPDDMDLNDSTKFYTYEVNLTFVGVVGMLDPPRKEVQDSIVRCRAAGIRVIVITGDNKATAEAICRRIGVFGEDEDTTGKSYSGREFDDLSVSEQREACSRARLFSRVEPAHKSKIVEFLQSMNEISAMTGDGVNDAPALKKAEIGIAMGSGTAVAKSAAEMVLADDNFSSIVAAVEEGRAIYNNMKQFIRYLISSNIGEVVSIFLTAALGLPEALIPVQLLWVNLVTDGLPATALGFNPPDLDIMTKPPRKADEGLISGWLFFRYMAIGGYVGCATVGGAAWWFMFSETGPQLSYWQLTHHLSCLGGGEEFKGIDCKIFNDPHPMTMALSVLVTIEMLNAMNSLSENQSLVQMPPWCNIWLIASMCLSFALHFVILYVDVLSTVFQVTPLDGNEWMTVMKFSLPVVLLDEILKFVARRISDVNPDFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VPC4</accession>
    <name>A0A6E8VPC4_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein O-GlcNAc transferase</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VPC4"/>
    <dbReference type="EnsemblMetazoa" id="ACON006254-RA">
        <property type="protein sequence ID" value="ACON006254-PA"/>
        <property type="gene ID" value="ACON006254"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000731"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON006254"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033711"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097363">
        <property type="term" value="F:protein O-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000252">
        <property type="entry name" value="O-linked N-acetylglucosamine (GlcNAc) transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037919">
        <property type="entry name" value="OGT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44366">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE--PEPTIDE N-ACETYLGLUCOSAMINYLTRANSFERASE 110 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44366:SF1">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE--PEPTIDE N-ACETYLGLUCOSAMINYLTRANSFERASE 110 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13414">
        <property type="entry name" value="TPR_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="179"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="213"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON006254-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="283" mass="31605" checksum="A13C30E89BC7AA7E" modified="2020-08-12" version="1">MSSVENAENGVGKYSYQTVYYIITNNNNNNSTEEGESDQVENLHAITAAVDSELTRKRRRIEAIKMQGQIPGAAAVQVQGGTPQAAALAAQQQQQQQSISVKMDPANQLSSAGLLELAHREYQAVDYDNAERHCMQLWRQESNNTGVLLLLSSIHFQCRRLDKSAQFSTLAIKQNPLLAEAYSNLGNVYKERGQLQEALENYRHAVRLKPDFIDGYINLAAALVAARDMEQAVQAYVTALQYNPDLYCVRSDLGNLLKALGRLDEAKHEMPHTHNFSLLYCMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VPC6</accession>
    <name>A0A6E8VPC6_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Mitogen-activated protein kinase kinase kinase kinase</fullName>
            <ecNumber evidence="10">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Serine/threonine kinase that plays a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8VPC6"/>
    <dbReference type="EnsemblMetazoa" id="ACON006340-RC">
        <property type="protein sequence ID" value="ACON006340-PC"/>
        <property type="gene ID" value="ACON006340"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012613"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON006340"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037044"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008349">
        <property type="term" value="F:MAP kinase kinase kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06608">
        <property type="entry name" value="STKc_myosinIII_N_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000003">
        <property type="entry name" value="TRAF2 and NCK-interacting protein kinase isoform 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000006">
        <property type="entry name" value="TRAF2 and NCK-interacting protein kinase isoform 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001180">
        <property type="entry name" value="CNH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021160">
        <property type="entry name" value="MAPKKKK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051700">
        <property type="entry name" value="STE20_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47096">
        <property type="entry name" value="MISSHAPEN LIKE KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47096:SF1">
        <property type="entry name" value="MISSHAPEN LIKE KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00780">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038172">
        <property type="entry name" value="MAPKKKK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00036">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50219">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="21"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="CNH" evidence="16">
        <location>
            <begin position="753"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="304"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="358"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="364"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="386"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="422"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="488"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="506"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="14">
        <location>
            <begin position="523"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="553"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="14">
        <location>
            <begin position="625"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="663"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="692"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="727"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="11">
        <location>
            <position position="149"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="27"/>
            <end position="35"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12 13">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR038172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR038172-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR038172-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON006340-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1070" mass="117952" checksum="50B784E7BB75F240" modified="2020-08-12" version="1">MWFTDILLSFSLSLQDPAGIFELIEVVGNGTYGQVYKGRHTKTGQLAAIKVMDVTEEEEEEIKLEINVLKKYSNHRNIATYYGAFIKKTPAGKDDQLWLVMEYCGAGSVTDLVKSTKGQSLKEEWIAYICREILRGLSYLHTNKVIHRDIKGQNVLLTDNAEVKLVDFGVSAQLDKTIGRRNTFIGTPYWMAPEVIACDENRDATYDNRSDLWSLGITALEMAESQPPLCDLHPMRALFLIPRNPPPRMKSKKWSKKFHSFIDTVLVKDYHQRPYTEQLLKHPFIKEQPTERQVRIQLKDHIDRCKKRKQEKERDDYRYSGSENDDDDGQTAGEPSSIIQAPGNDTLRRTFHQIQEGRMQNAEQQQPPNRNQKPQPRDERSKPQPVEEPGPPSRPQLPQRLIVVPDPPANSNANRPLPPTPRSGSGSSSQPQQPSSQTPQQPARNQQNFFKPVVSSGRATPLYTQSQQAQPEAPPRNNRPQPGPPVPGSQQQQQQVSAGGVGASGVGKAPAIAPNGNNNSSNGAGGGSGGGVGGNINNNNHHPQPINPLDPIESSDSDSEPEEPNGRTRNDGTLLASDPPMPLPEFSYRTGGLGVLAESDQNNQSSSSTPAGGGGASGASVLSPPGGGGGGGGGPPNRPLPPTPDDDDAQGDGTLIKRKNRSGENSSLGTPGQRTSSVLPDLLSQASPATPPRHDKSASEEKQRSFLTFGFGAQSGGSAASRRESHVNVNVTPTSHDATSDTPEIRKYKKRFNSEILCAALWGVNLLIGTENGLMLLDRSGQGKVYQLISRRRFQQMEVLEGQNILVTISGKKNRVRVYYLSWLKSKILRTDGLTDQQVERRNGWINVGDLQGAVHFKIVKYERIKFLVIALKDSIEIYAWAPKPYHKFMAFKSFGELMHRPLLVDLTVEEQTRLKVIYGSAEGFHAVDLDSATVYDIYLPKHTQGPISPHCIVTLPNSNGMQLLLCYDNEGVYVNTMGRVSKNIVLQWGEMPTSVAYIGTGQIMGWGNKAIEIRSVETGHLDGVFMHKKAQRLKFLCERNDKVFFSSAKGGSSCQIYFMTLNKPGMANW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VPD0</accession>
    <name>A0A6E8VPD0_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="10">MFS domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Spinster (TC 2.A.1.49) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VPD0"/>
    <dbReference type="EnsemblMetazoa" id="ACON006346-RB">
        <property type="protein sequence ID" value="ACON006346-PB"/>
        <property type="gene ID" value="ACON006346"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006697"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON006346"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040560"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17328">
        <property type="entry name" value="MFS_spinster_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044770">
        <property type="entry name" value="MFS_spinster-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23505:SF79">
        <property type="entry name" value="PROTEIN SPINSTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23505">
        <property type="entry name" value="SPINSTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="155"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="182"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="206"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="241"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="272"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="323"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="368"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="401"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="428"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="553"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="117"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="597"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="20"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="52"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON006346-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="618" mass="66245" checksum="17413D70EA6AD858" modified="2020-08-12" version="1">MPSSQGYSKVASEPPIDPVDSSTADSSSMPAGGGNVGAAATAIMGKAPASGGIQSNPAGKIPATNSQQRLMPTDSDSVTSSMDEGELRVARGNPHDEDTDTVTTTTTSSIGANAWFTVGVLCFVNLINYMDRFTIAGVLTEIQDHFKIGDDEGGLLQTAFVLSYMVFAPLFGYLGDRYSRKWIMVLGVSLWSTTTLLGSYMDHFGWFITFRALVGIGEASYSTIAPTIISDLFVGEMRSRMLALFYFAIPVGSGLGYIVGAKMASIMNSWVWSLRVTPVLGAIAVVLIVMLRDPQRGQSEGTHHMQTTSYKEDVKAIMRNRSFMLSTAGFTCVAFVTGALAWWGPKFIHLGLISQPGNEHVTLNEVSFIFGAITMTTGIIGVPLGSYLSQRLNAKNVKADPYICATGLLLSAPLLVGAMFSVRANIYATYALIFFGELALNLNWAIVADILLYVVVPTRRSTAEAFQILISHAFGDAGSPYFVGVISESIKRVLRIVHDAVATDAYHPSVESSSSHSIAVLSQLAENSTVAMLQAASPPAEDSPLVQFRALQYALFSTCFVEILGGVFFLVTAFYIDRDRARVEAVVLGNQSNNASAHQLGGSVSDMEDALPPPSTRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VPE8</accession>
    <name>A0A6E8VPE8_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Iron-sulfur clusters transporter ABCB7, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">ATP-binding cassette sub-family B member 7, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(glutathione)4[2Fe(III)-2S] cluster(in) + ATP + H2O = (glutathione)4[2Fe(III)-2S] cluster(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:67028"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:167627"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:67029"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VPE8"/>
    <dbReference type="EnsemblMetazoa" id="ACON006364-RA">
        <property type="protein sequence ID" value="ACON006364-PA"/>
        <property type="gene ID" value="ACON006364"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_014470"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON006364"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037903"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18582">
        <property type="entry name" value="ABC_6TM_ATM1_ABCB7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000004">
        <property type="entry name" value="ATP-binding cassette sub-family B member 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000186">
        <property type="entry name" value="ATP-binding cassette sub-family B member 7, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221:SF402">
        <property type="entry name" value="IRON-SULFUR CLUSTERS TRANSPORTER ABCB7, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="136"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="167"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="250"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="292"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="13">
        <location>
            <begin position="22"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="348"/>
            <end position="582"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00041016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00042945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON006364-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="602" mass="66454" checksum="D1DED0A8889BCC77" modified="2020-08-12" version="1">MIKAMATYIWPKDDAMVRKRVLISLGLLGGAKVLNVCVPFLFKMGVDNLNVLSMDTVPQAASALTISVLLGYGIARAGAAGFNELRNAVFARVAQHSIRKIATNVFLHLHNLDLQFHLSKQTGALSKTIDRGSRGINFVLTAMVFNVVPTMFELALVSSILGMKCGMAYAALSMGCVGVYSAYTLAVTQWRTKFRIYMNQAENEAGNKAVDSLINYETVKYFNNEQYEANRYDQVLKKYEDASLKTSTSLALLNFGQNAIFSVALSTIMVMAANEIAQGRMTVGDLVMVNGLLFQLSIPLGFLGSVYREVRQALLDMRTMFTLMGVQSAIQSRANAPPLDVRRETASIEFRNVGFRYAQSNEIFKDLSFTIPAGKKIAIVGGSGSGKSSMVRLLFRFFEPSTGEILINGQNIREVDLQSLRKAIAVVPQDSVLFHDTIRHNIHYGDLSKSQTELENAARLADLHDSILQWPKQYETQVGERGLKLSGGEKQRVAIARAILKNSPILIFDEATSSLDSITEQNILQALARATDNRTSICIAHRLSTVMDADEILVLENGRVGQRGTHDQLLRGGGLYTKLWDTQNRLYNIGSKKPTKPEGEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VPF4</accession>
    <name>A0A6E8VPF4_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">2-oxoglutarate dehydrogenase complex component E1</fullName>
            <ecNumber evidence="6">1.2.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="24">2-oxoglutarate dehydrogenase complex component E1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20 22">2-oxoglutarate dehydrogenase, mitochondrial</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Alpha-ketoglutarate dehydrogenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">Thiamine diphosphate (ThDP)-dependent 2-oxoglutarate dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="27 28">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="27">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="19">The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[protein] + 2-oxoglutarate + H(+) = N(6)-[(R)-S(8)-succinyldihydrolipoyl]-L-lysyl-[protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:12188"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:20092"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83120"/>
            <dbReference type="EC" id="1.2.4.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:12189"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the alpha-ketoglutarate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.4.2" evidence="6"/>
    <dbReference type="RefSeq" id="XP_049464001.1">
        <property type="nucleotide sequence ID" value="XM_049608044.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VPF4"/>
    <dbReference type="EnsemblMetazoa" id="ACON006366-RB">
        <property type="protein sequence ID" value="ACON006366-PB"/>
        <property type="gene ID" value="ACON006366"/>
    </dbReference>
    <dbReference type="GeneID" id="120949786"/>
    <dbReference type="CTD" id="136036099"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006013"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON006366"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033405"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045252">
        <property type="term" value="C:oxoglutarate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004591">
        <property type="term" value="F:oxoglutarate dehydrogenase (succinyl-transferring) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02016">
        <property type="entry name" value="TPP_E1_OGDC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12470:FF:000007">
        <property type="entry name" value="2-oxoglutarate dehydrogenase e1 mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000002">
        <property type="entry name" value="2-oxoglutarate dehydrogenase, E1 component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11610:FF:000003">
        <property type="entry name" value="2-oxoglutarate dehydrogenase, isoform X4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1150:FF:000001">
        <property type="entry name" value="2-oxoglutarate dehydrogenase, mitochondrial isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11610">
        <property type="entry name" value="Multifunctional 2-oxoglutarate metabolism enzyme, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1150">
        <property type="entry name" value="TPP helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032106">
        <property type="entry name" value="2-oxogl_dehyd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011603">
        <property type="entry name" value="2oxoglutarate_DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001017">
        <property type="entry name" value="DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042179">
        <property type="entry name" value="KGD_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031717">
        <property type="entry name" value="ODO-1/KGD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00239">
        <property type="entry name" value="2oxo_dh_E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006914">
        <property type="entry name" value="PRK09404.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008907">
        <property type="entry name" value="PRK12270.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23152:SF4">
        <property type="entry name" value="2-OXOADIPATE DEHYDROGENASE COMPLEX COMPONENT E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23152">
        <property type="entry name" value="2-OXOGLUTARATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16078">
        <property type="entry name" value="2-oxogl_dehyd_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00676">
        <property type="entry name" value="E1_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16870">
        <property type="entry name" value="OxoGdeHyase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000157">
        <property type="entry name" value="Oxoglu_dh_E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="16" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="7" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="12" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="11" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Transketolase-like pyrimidine-binding" evidence="26">
        <location>
            <begin position="671"/>
            <end position="884"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00030680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00037426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00040267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00040429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00041946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00042799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00042984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00051042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON006366-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1034" mass="116348" checksum="EC0D93426289C78D" modified="2020-08-12" version="1">MHRARTALHMVNPMGQQNFGSFLLKKQASKLTTELVAASSVKLYNSAAAEPFLNGSSSNYIDDMYNAWLRDPASVHASWDAYFRNNSYAAPPSLAPVPKNHVPAAQYLGSSLPAVAGAGAAVGGRIDDKLIDDHLAVQAIIRSYQIRGHNVARLDPLGINSADLDDKTPPELLYSSYRFVHQRILECVSNEDRQLLLGMKEADMERVFKLPSTTFIGGKEKFLPLREILGRLEKAYCNKIGVEFMFINSLEQCNWIRERFETPNIMTYTNEEKRLILARLTRATGFEAFLAKKFSSEKRFGLEGCEIMIPAMKEVIDVSTRLGVESIIMGMPHRGRLNVLANVCRKPLHQIFTQFAGLEAADDGSGDVKYHLGTYIERLNRVTNKNIRLAVVANPSHLEAVDPVVQGKTRAEQFYRGDGEGKKVMSILLHGDAAFCGQGVVFETMHLSDLPDYTTHGTIHIVVNNQIGFTTDPRHSRSSPYCTDVARVVNAPIFHVNGDDPEAVMHVCKVAAEWRATFHKDVIIDIVSYRRNGHNEIDEPMFTQPLMYKKIRGTKPALDIYANQLITEGVVTAEEVKSVKDKYEKICEEAFEQAKIETHIKYKDWIDSPWSGFFEGKDPLKVAPTGVIEETLVHIGNRFSSPPPNAAEFVIHKGLLRVLAARKEMLENKTIDWALAEAMAFGSLLKEGIHVRLSGQDVERGTFSHRHHVLHHQTVDKATYRPLCHLYPDQAPYTVCNSSLSEFGVLGFELGYSMTNPNALVCWEAQFGDFNNTAQCIIDQFVSSGQAKWVRQSGLVMLLPHGMEGMGPEHSSARVERFLQMCSDDPDYFPPESEEFAIRQLHDINWIVANCSTPGNYFHLLRRQIALPFRKPLIVLTPKSLLRHPECRSNFSEMTDGTEFKRLIPDALTAENPNQVKRVIFCTGRVYYDLLKARRDRKLDHDIAISRIEQISPFPYDLVKAECAKYPNAELVWAQEEHKNQGCWTYVQPRFDTAINSTRDFSYVGRPCGASTATGSKAQHLKELKNLLDDAMAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VPH4</accession>
    <name>A0A6E8VPH4_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Domino</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family. SWR1 subfamily.</text>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON006165-RB">
        <property type="protein sequence ID" value="ACON006165-PB"/>
        <property type="gene ID" value="ACON006165"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002862"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON006165"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031648"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000812">
        <property type="term" value="C:Swr1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140096">
        <property type="term" value="F:catalytic activity, acting on a protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010557">
        <property type="term" value="P:positive regulation of macromolecule biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18003">
        <property type="entry name" value="DEXQc_SRCAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="helicase SRCAP isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000005">
        <property type="entry name" value="Photoperiod-independent early flowering 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014012">
        <property type="entry name" value="HSA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050520">
        <property type="entry name" value="INO80/SWR1_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45685:SF1">
        <property type="entry name" value="HELICASE SRCAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45685">
        <property type="entry name" value="HELICASE SRCAP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07529">
        <property type="entry name" value="HSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00573">
        <property type="entry name" value="HSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51204">
        <property type="entry name" value="HSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
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            <begin position="696"/>
            <end position="768"/>
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    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="14">
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON006165-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="19">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="3189" mass="341669" checksum="CA3AC22147CE25DB" modified="2020-08-12" version="1">MNEGDTAGGQRGRNQALPAGIDRNTSTATNTVQLPTGTLDSITPMSRSLNEDQQQQLEQSQQQQQQQQPQHHQQQQQQQQQQQQQQQQQQQQQGQQMQPAGTPTSSSSSLSSPHKSLPQQQQQQQPSPSSNAAGGNVTVATDASSVPLSSSAPSHHTLQQQQQNVANSASSSSSSSSGGGTSSGSVEANNANITSTPGGERTGSGLSESEGGGRGGRGGGSIASGGVVEGGLVPASGASGGGGSTSETPGSEESTFGVVVGEKRAIEDSTGSVTDVAGGGGGGASEEGGIVGASGAVGKLYTKTNTTASTSSSSSSSSFSPVVAGGIAVGGQAAGGTVSAAVAVAAASVSNKRIKLETEPEHDISALKKLILEHKYMRLRSIKERYNEHVAELFFLQTGGNMMEYPVWRKKPPTPAFTSFVRSYKLEPLSSNLEEAGEALKQQSTRALSGFSTTPGSTNLGSVVTPVVVSGNGSSSSSGGDDTLTGLPQGAEIKIPGVGATPVAVSTTLPAAVAQLSQQGGTPIIPDRVGIGISPAAASAVDRKRQISSASSALGGHMIKSELLETANASPSVTTIGTNGPNVPSVRQGGSLTGGAGGSRGSGSTAGGSITGSSSRGLNNGQQNGAVGNGRGNALSSGSLAGQQQQQQQQLGGGVPAGTNSNGPPDQYTNKARQEVFVMQRIQELQKEGLWTEKRLPKVQEPPRPKAHWDYLLEEMVWLAADFAQERKWKKAAARKCARMVQKHFQDKALAAQRAEKAQELQLKRIAAFVAKEIKTFWSNAEKLVEYKQQTKLDERRKKALDQQLSFIVDQTEKYSQQLVEGMNKAAPVPGGAAAALLPPGSKAESLNSSRLSSPLRGSGGLAGGSDDEFCPEAESSDDDEETIAKAEAEAAAEAEDGTKDEVAALQKESEMDLDDFLKNLPKDYLENRDKIVLSDSNDGDGDGEEEQEEEEEEEEADEKRRKKKQGATREGEEEDGDKDFSADEDSMDDEDTIREQEKKEKNQDHKKEIDELNADNDLTIEELMAKYKNLPPAGEQMDIDSDDDADEVPAVPANDRKRRRTRSQPMYESSDDEDKEEEDDNDESEGLTGSDDDEEEEDDDDITEDESDESEEAMDAEEDAFGLKHLLDDETGSGETAATSTAAGSSGASSAAKSQSDKDDMLNDAAAIAESIQPKGNTLSSTSVVTPIPTLLKHSLREYQHIGLDWLVTMHDRKLNGILADEMGLGKTIQTISLLAHLACVKGNWGPHLIIVPSSVMLNWEMEFKKWCPGFKILTYYGTPKERKQKRTGWTKVNAFHVCITSYKLVIQDHQSFRRKKWKYLILDEAQNIKNFKSQRWQLLLNFQTEQRLLLTGTPLQNNLMELWSLMHFLMPHVFQSHREFKEWFSNPMTGMIEGNSEYNETIIKRLHKVLRPFLLRRLKCEVEKQMPKKYEHVVMCRLSKRQRFLYDDFMSRAKTRETLASGNLLSVINVLMQLRKVCNHPNMFEERPTISPFRMEGISIKTASLVYNMFSYDPFTQIDLSSLNLVLIQLELLLPAYVAHRAQHLCMPKRLIEEIDSTPLPPPRCPTGKLRLHVRIPDVRVQESLGGNRAGVSITTGVRVGTSPAMKTEGTKFVPLVNHEASLDKKSSLIGSGGSSSSTIQSRVIDIRRRGSSTVMMMMGGGSSAGLDSSSMVAAAGSNRSMSPGLLLRKRSDIAGGGGIVSMAGPLHQQQQQNRLNAGQVVQIIPQVSSGGGIGTNASQSYIITGRLQPAAGGSGGQAIFHRPALAAGGSGGVNVLPTQRIATHQSMGGQSSAAATSAAAAAAATVSGATANRMVQNVGQKTQISSTLAATIMKQLKHSKSVAGSSGDAAARSAEAAEMDERQRTEFYLACIEESRKERRREVLELLGRINAKRCDATPIYGRDLRETLCALIEEEFRHRPDELIPFGVASSYHTRRATMARCGGGGGCGGTKNGALFAAASSCLSEAIKSIDQRAGELRATISNFVLFVPAVCAPTPYLQVSHPHPSKLNAEQQWEATMAEQIQPAIQLLHPIISAMSTQFPDPRLIQYDCGKLQTLDRLLKKLKSEGHRVLIFTQMTRMLDVLEAFLNYHGHIYLRLDGTTKVEQRQVLMERFNNDRRMFAFILSTRSGGVGINLTGADTVIFYDSDWNPTMDAQAQDRCHRIGQTRDVHIYRLVSEKTIEENILKKANQKRILGDLAIEGGNFTTAYFKSSTTIQDLFTVDSAEQDASARLAEVLDRDRERKERLNANLATGPGQPQPATGGEPAVDSSKSAINVFESALAAAEDDQDVQAAKFAKAEASADLDEFDETIPIDEEKVAAAGDAEGKEPELSKAEKEVQNLIKQLSPVERYAMRFVEDTEGTWTAVQLKAVEQEIEQQKRDWEANRLAEQRRNEEAARKREAEEHADLLTFSREDAKNQVNKTHPSNRVSSGGSSKHRLRTMRRNDRTSRVTTATTTAKTMTNRDGGTLKNRANSRGHQVAASKYDGGSRSGGRADGGQKLTTATGRRVLASAKQQSKHHSGGVGAKQSGLGNNKATGSILGKRKYQESAVAKGGARSQSSKAAVAAAANQRRVKGATRNAKHARRNVSKASLTDDEDEDEDEDELESDESSNETVQDEGESNREENGSDSEAADSGEDGTEEEEEEEQDPLADVGTEDDRGKKQDDAVQDGENAAIVMLRNTPRRASVAARAVIGSMIQKTGVNVKQSPKAATTTNSGGSGGGGSARAKITRQVPSPVRVAKSSNNAAADRSTVSSASSSSASDAAAAVTASSLAKPLDEFDSECSLDVMVDSNDAPDSDSNQMNSYYNSGNSAGPGMHHDEDARAGGTVDGGGGGGGSSTKDHQSSRPTSRVGRARDGRSRTPVVSSTSPMVVPASCDLSVDETSDDVDESSNASKTTPRTRSRGTVKINLWTLDDSPMLPVYNRRPNRSKTPQLSQQSNHHHHVLGDDGGDTEERESAECSKKDASSSAVESSSSKPAEANECNSSTVVDNHRDHLNHSSDSSAAPLPPATTTEQQQPQKTVTNSIVPKTTNVIRNLKVVVNDLLRTPDTAKRVAEVSDGFRAPTPTNTKLRRLKRLSRGVGSAGGRATPSIVMNAGGATYNRTLDGWLTKAGTTGAGRKEEADGGSENHRSGGADGKLPGGEEGSASKLAPTVARSRTAIAAAGEGQQHEEKDPLENGNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VPH7</accession>
    <name>A0A6E8VPH7_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VPH7"/>
    <dbReference type="EnsemblMetazoa" id="ACON006347-RA">
        <property type="protein sequence ID" value="ACON006347-PA"/>
        <property type="gene ID" value="ACON006347"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004614"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON006347"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035010"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000017">
        <property type="entry name" value="potassium voltage-gated channel subfamily KQT member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000016">
        <property type="entry name" value="Putative potassium voltage-gated channel subfamily KQT member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1910">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003937">
        <property type="entry name" value="K_chnl_volt-dep_KCNQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013821">
        <property type="entry name" value="K_chnl_volt-dep_KCNQ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47735">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL SUBFAMILY KQT MEMBER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47735:SF9">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL SUBFAMILY KQT MEMBER 4-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03520">
        <property type="entry name" value="KCNQ_channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00169">
        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01459">
        <property type="entry name" value="KCNQCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0630">Potassium</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="218"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="246"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="292"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="357"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="418"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="15">
        <location>
            <begin position="218"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel voltage dependent KCNQ C-terminal" evidence="16">
        <location>
            <begin position="564"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="519"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="685"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="915"/>
            <end position="971"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="49"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="70"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="114"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="706"/>
            <end position="719"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF03520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON006347-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="971" mass="107902" checksum="41B13D47BF13F554" modified="2020-08-12" version="1">MRSTAMEDVYENEPHAPDPAEQPKPFNGSSGKNVSLRILSMPSSPLPPVAGAVPSSPSAVSLSSTNARDTRHRSSKDRVSRKARVSTIASPGASGRTDLTPGVTTIDEGGASDGGNTPSSLAPCSSTVKKSKVDFVRFDSTEIHYTENEEVHKKISDEIKSACKLSDKNRADRLARPRMSLLGKPLNYNRGSRRDARYRRLQSRVYNFLERPRGFKAIFYHVCVFLMVFTCLALSVFSTIQEYEDQAIAILFVMEIIVVIWFSIEFFLRLWSSGCRSRYQGAVGRLKFLKRPFCIIDIVTIAASIVVLVMGTSGQVFATSALRGLRFFQILRMVRMDRRGGTWKLLGSVVYAHRQELITTLYIGFLGLIFASFLVYLMEKDVRGTKFSNFAQALWWGVITLCTVGYGDMVPETWQGKIIASFCALLGISFFALPAGILGSGFALKVQQQQRQKHMIRRRQPAATLIQSLWRCYAADEHSMSEATWKIHQVPLPSPPPSRASSSFKHNASFVARLPTIRRHKSQSLHSPGNPKPPSGGSGRTGRCPRVTDINASSENLDEDEEPRCVQLTNQHKGAIRFIRKMKYFVARRKFKEALKPYDVKDVMEQYAAGHVDLLGRVKNVQTRLDQILGKQGSKSKDVYASKISLASRVVKIERQVDDIETKVDSFIDLYMQDRMRLLSLPLHPDTSHSNNPNLPSLPPKGGGASAVITSVTTSSSGSLKPKPILVDKQFSEPNSPIAKTFEEQPLQQKRPPMQRGFSDLGHRIKKRVTLSSIPPQYVGNSTGQPSDRGDVVIVVPNIDAEHLEPVGIEELASVCIDTEIEIEPGSPKTITESSIIMMDEEDDEEDEEEDIEEEDLDIGAEIDPDSPPWDVYGDMDVEDQDDTTENTALLRTAAKTEIIVTPISPVSSAFELNRMDSEEYRRSKQVAANNNEGEQHQQQQQQQQMLAITGPSGAMDSLKPERASNGSDDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VRH3</accession>
    <name>A0A6E8VRH3_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">T-lymphoma invasion and metastasis-inducing protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
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        <property type="protein sequence ID" value="ACON006590-PD"/>
        <property type="gene ID" value="ACON006590"/>
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        <property type="component" value="Unplaced"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:small GTPase-mediated signal transduction"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="PDZ_canonical"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PH1_Tiam1_2"/>
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        <property type="entry name" value="PH2_Tiam1_2"/>
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        <property type="entry name" value="RhoGEF"/>
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        <property type="entry name" value="Protein still life, isoform SIF type"/>
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        <property type="entry name" value="T cell lymphoma invasion and metastasis 1"/>
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        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
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        <property type="entry name" value="DBL_dom_sf"/>
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        <property type="entry name" value="GDS_CDC24_CS"/>
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        <property type="entry name" value="PDZ"/>
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        <property type="entry name" value="PH-like_dom_sf"/>
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        <property type="entry name" value="Tiam1/Tiam2/Sif"/>
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        <property type="entry name" value="TIAM1_CC-Ex"/>
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        <property type="entry name" value="PROTEIN STILL LIFE, ISOFORM SIF TYPE 1"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR46001">
        <property type="entry name" value="TIAM (MAMMALIAN TUMOR INVASION AND METASTASIS FACTOR) HOMOLOG"/>
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    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1112"/>
            <end position="1225"/>
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        <location>
            <begin position="1391"/>
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            <begin position="1474"/>
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            <begin position="1697"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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        </source>
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    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
    <sequence length="2268" mass="250797" checksum="B170F4570E74AADD" modified="2020-08-12" version="1">MSSSGSDEPHAPNGTPHPHRHYYTALRTAPLLILAALQQYTTATDQQQQQQPNHRAPDTATTACNGSAGENGRAGYVEQQQPPQGKSPNTLLLHCCCVIVQITPVAPGRSHVTTTASAKKPTIGDDGGGGAGGGGGGASGRTPVCRCNAQYKLQDLKALAAAQQKQQRLAAAQSASTSAINAIGTGGGGGGGGGASAAAPPLQGILAKSSTTTSGHHAHRAHQPLQQQHSSQSLAYSTGGAGPPQPPPRQSQELSNEELIKFLQQINADIKWIEQLQQYKYEIEKSTSTDKTNARNINKPTGQQQQQQHQQSVPPLANGHLGKPKSEPLLPTVAAPAPSTGAISKTEFFDRNLKQRHSSRSVDASTARRDEEAHGKEGGHQRHSSGPSDLQLLKSDISEWLLNLPLKKSVEILQKAKSAQLQYEKEQELQLQLQQLAAKKPLQRQTSSGTAERGGTGQQPGEIALTRDDILEWLKKQQIFSKVNEETGATKKCKDKEELTLHHYDPSRAPRGPAPSSASTAKLLKRHSLGPNEDFFQKQPTTTDWIQYPLRQLKKHTFDPATQSSCTDVRRKEVRGEHGHSHRERKLRHSASEVVTSSQSDLPVHSQSQTRIAPAKPERTFRYHSRERAPSAHSAAPTHRQHSMPQIPLHQQQQQQQPLHFQYPPALQPQPSTSTFVPPAAGHRSAKKERSRRHRTQRSATVGDINQFNKPSAQPPVGGYLHHPKAGPHARATAAAERSASLTRCADPSCSVMSLCDDPTCYLFGSGEDGALAPVVGTANATTSYYDTPRCASLPRVLPKSAPKCGPLCYECNNKCNSLPRCTDARCQMGGGGGGGGGGLPTTKAKSNSLPRNAAVHHYPAAAAMYPAVPQHQAPAAPPPCQPALRGAGRLSREESRCSLPRSSFKSSKKRSASASGSGSNGSKLTKSLSAASLNSRRRRHKTVHFGENLLREVCQNRKLIQPLQETPSGSAPLQPNIQMLYNFVEGVLSAWVDDEDDPNKSGPESEPERGAVLKPMHRCNRARFQTIRRVVNEAAELKGSSRLGNSRYRHRHWRGTAKDCNERFLRKISDDDRMSLTTAVSDEDDGESVMASPYKAKATGTAAASFNCTGAVRKAGFLSVKKWLLRKKHQIELARKRGWKGYWVCLKGTTLLFYPCDSREGRSVEAAPKHLIIVDGAIMQPIPEHPKRDYIFCLSTAFGDAYLFQAPCQVELENWVNSIHSACAAAFARHRGKTGTLHLLQEEIFRLEKAIESDHKLKHMADLQQSVVTDADTRHQIQQQILQWEENLERLHCEQFRLRCYMASLQNGELPNPKSLLTHVSRPTKNTLNKLGVFTVSSFHAFICARSPSLLNNLLAGRGATKRRPPILSRSNSGSSRRSMQINSRDEPEKSYKVALPENTFATVYLREGMSVEEFLASACSRKNLNPMEHFVRVKKRRDMEDHNYFVPHRNDLIETYLHTHEVVEVCAKILYQVELQRTTLEQMWGFSVEAELIENADRQDELCCYVSRVEDKSVAMQNGVIKGDEIIVINGAIVSDLDMMYLESVLQEEQALCMMMRSSRTEPPDLVGIMRSTDDIIESLVCPPPPSDPPVISEEMISGLIVPAPGWRKEVYSPEVESSPAPSSNDPHLLSMDRSKPSSRTSSFEIENLLKSAEQVTGFCRSPQETRKSSPTGSVASSVSNVMLTPSRQLTDAEKLRKVILELVDTEKSYVKHLNNLLEYYLEPLKRETFLSNAEITALFGNIQEIVTFQRQFLNNLEEALDLEPDFHQLEHPSQFKNVLFAIGSAFLYYVNHFKLYSSFCASHSKAQKVLHPNEGNQALQEFLAARNPKQQHSSTLESFLIKPIQRILKYPLLLQQLRNLTDPNAAEHLHLVEALKGMEKVAEHINEMQRIHEEYGAIFDHLFRQHQKSCKQPIDLSPGDLLYYGGVEWLNISDFLGKIKKGLELHAMCFVFKSAVVFLCKERLRQKKKLMGVSSKNATNEVEIIRYQVLIPVTEVQVRASSAKDMDSHFLWELIHLRSQLQRRSEKVYVLSNSTAEFRNAFLKTIRQIIRESVRNMSIPSLKSMGGSTNSMTGVGNSSSGNSQTLERPKQQPQIMQGSQTLGKPKKSKINQRHSSGNIDYDNTTSGSQEADDPPVPQYSGQFRMRSKTVGDVGEQVAACRRNMNEREQEKPDPGTKSEGEDDSQQGTVKPKSSLGKTPNHLTLSTTSTLSVGSTGSQARLIQSSHPPATYHPVLMKDLGSPVWKPRDMISLSDAPSTSRKDESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VRH4</accession>
    <name>A0A6E8VRH4_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phenoloxidase-activating factor 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Prophenoloxidase-activating factor II</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase S1 family. CLIP subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VRH4"/>
    <dbReference type="EnsemblMetazoa" id="ACON006954-RA">
        <property type="protein sequence ID" value="ACON006954-PA"/>
        <property type="gene ID" value="ACON006954"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002731"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010361"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON006954"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031597"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035592"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000038">
        <property type="entry name" value="Serine protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24258:SF142">
        <property type="entry name" value="PEPTIDASE S1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24258">
        <property type="entry name" value="SERINE PROTEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026346228" description="Phenoloxidase-activating factor 2" evidence="8">
        <location>
            <begin position="22"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="9">
        <location>
            <begin position="665"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="135"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="575"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="140"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="155"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="581"/>
            <end position="607"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00068096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00076468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON006954-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="911" mass="97707" checksum="1B2BDC596D9A535C" modified="2020-08-12" version="1">MRLLGSAALLLVLSAVAVIGADEWGWLPLEDGLQGEEKVREKRQDIGVLPASQQNVTEVSDEDILKFILDSGRQGRSLEGFDEVYSDPSVQDALQNADDAQARNIIKDKLCSLGLMQCDGQGESIEGKRPYYPIYAQQQPPRPRPPVRGPPLPPQQYAQRPPQPPQQQAYPPKPQHQGPYGPPKPMPVPNHAQHKIGYAGPQQRPPYSGPPNSFAGPSYASAYPSKPLGPIYEGDTPPFEFEPIGAGEKIYESPLSKPGLVLNEGPLVKPVEQHIHHHYHHIDGAADSKTVVVNNPGPVGASVLSGSAVVGGSSSSSFNSGLYSAGGVYGGAQGLSGGGFSPLAGSDFDYKELKGVNNNNGFGQSGVYAAQSKPVFEGSSSSFGQGGQSSLYNQGPAQFGSNSLYSGESSSAYQGGNGAGFHSSNPALYKKELNVKGPAASNGLGTYSGNNYSKYSQYANGQYAANGGQFGSSGGQFGANGGQQFGANGGQQFGANGGQFGANGGQYSQFANGNGIEDCVCVPYDQCPAQDVIGRKDDLILPLDPRNLKTDIEAAADEVVITDGNGTMTVVRVPKNATAEPETKTKKISKREAAEGKSNEAKDKASIEPRQAYYGPRPGQQQQQCSGRQVCCRKPVYRNPASQNLGKCGVRNAQGINGRIKNPVYVDGDSEFGEYPWQVAILKKDPKESVYVCGGTLIDNLYIITAAHCVKTYNGFDLRVRLGEWDVNHDVEFYPYIERDIISVQVHPEYYAGTLDNDLAILKMDRPVDLTSAPHIAPACLPDKHTDFSGQRCWTTGWGKDAFGDYGKYQNILKEVDVPIVNHYQCQNQLRQTRLGYTYNLNQGFICAGGEEGKDACKGDGGGPLVCERNGVWQVVGVVSWGIGCGQANVPGVYVKVAHYLDWINQVRGRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VRM1</accession>
    <name>A0A6E8VRM1_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="6">SHSP domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3 4">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VRM1"/>
    <dbReference type="EnsemblMetazoa" id="ACON007160-RD">
        <property type="protein sequence ID" value="ACON007160-PD"/>
        <property type="gene ID" value="ACON007160"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004108"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007160"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033299"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06526">
        <property type="entry name" value="metazoan_ACD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055269">
        <property type="entry name" value="Alpha-crystallin/HSP_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001436">
        <property type="entry name" value="Alpha-crystallin/sHSP_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45640">
        <property type="entry name" value="HEAT SHOCK PROTEIN HSP-12.2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45640:SF34">
        <property type="entry name" value="PROTEIN LETHAL(2)ESSENTIAL FOR LIFE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036514">
        <property type="entry name" value="Sm_HSP_B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00299">
        <property type="entry name" value="ACRYSTALLIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="SHSP" evidence="5">
        <location>
            <begin position="57"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR036514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR036514-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007160-PD"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="175" mass="20534" checksum="0C45CA6E5D4D1660" modified="2020-08-12" version="1">MSLVPVQYRTWWDDWDLPLYTRVLEKSITQEVLGTDDYWRGAPLAPLRWSSLYRPWRYFSLRDVGAKVDTDRDRFQIELDVHQFLPHEVTVRRTDKYVTIEGKHEEKRDEQGYVARQFSRRYLVPIGYDANLIVSSLSSDGVLTVTAPRIGLPAPKVEKYVPIWHTGKPAIEDKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VRM5</accession>
    <name>A0A6E8VRM5_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Solute carrier organic anion transporter family member</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the organo anion transporter (TC 2.A.60) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VRM5"/>
    <dbReference type="EnsemblMetazoa" id="ACON006637-RA">
        <property type="protein sequence ID" value="ACON006637-PA"/>
        <property type="gene ID" value="ACON006637"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_008832"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON006637"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030154"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015347">
        <property type="term" value="F:sodium-independent organic anion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:monoatomic ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043252">
        <property type="term" value="P:sodium-independent organic anion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17336">
        <property type="entry name" value="MFS_SLCO_OATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:001137">
        <property type="entry name" value="Solute carrier organic anion transporter family member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.30:FF:000111">
        <property type="entry name" value="Solute carrier organic anion transporter family member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002350">
        <property type="entry name" value="Kazal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036058">
        <property type="entry name" value="Kazal_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004156">
        <property type="entry name" value="OATP"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00805">
        <property type="entry name" value="oat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11388">
        <property type="entry name" value="ORGANIC ANION TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11388:SF76">
        <property type="entry name" value="SOLUTE CARRIER ORGANIC ANION TRANSPORTER FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03137">
        <property type="entry name" value="OATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100895">
        <property type="entry name" value="Kazal-type serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51465">
        <property type="entry name" value="KAZAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="55"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="94"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="121"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="186"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="231"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="269"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="343"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="382"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="414"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="domain" description="Kazal-like" evidence="9">
        <location>
            <begin position="446"/>
            <end position="495"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON006637-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="500" mass="54753" checksum="2E6E3ED7EC604EB2" modified="2020-08-12" version="1">MNGSLANGLNEGHLNAPPTKIAFDRSISAYSERDVQCGFWVCRGPFWQRLASKKLYVLLFGLMGCILNSTTSYFYGTLTTVEKSIQISSRTVGIITAGSDLSFVLASLFLSYYASNRRKPLWIAMGIVSMALSCFVNALPHFLFSKAQAELEQNLGNLTKGADNLCREDRLSPDCSLDVGDIRPQIVLFFGSFLSGIGTSLYFTLGLTYLDDNVRKEKVPFLSSLAAFGRRMGPLVGFSLASLCLKYFVHVGVDPGYNQSDPRWIGAWWMGWLALGSTLIIIAPFFASFPKVLPRTAERISLSRQASRASGTSTAEEDVETNRKVSFSDMLVTVRRLVTNKAYVFNNVASIFYFFGYMPYFLFQAKYIEIQYRLTPSQANMITGSTSLVFSALGILIAGMVIQKLRVSSRQLAGWNVITSILSAALIVGYAWFGCSALNNADIVANSQDASCSLGCNCEFVKYAPICGSDGQTYLSPCHAGCRDSLKDPTGTTVGDTAFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VTL5</accession>
    <name>A0A6E8VTL5_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Enolase</fullName>
            <ecNumber evidence="3">4.2.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">2-phospho-D-glycerate hydro-lyase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">2-phosphoglycerate dehydratase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">Ngousso</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="12">Mg(2+) is required for catalysis and for stabilizing the dimer.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the enolase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.11" evidence="3"/>
    <dbReference type="RefSeq" id="XP_040240027.1">
        <property type="nucleotide sequence ID" value="XM_040384093.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VTL5"/>
    <dbReference type="EnsemblMetazoa" id="ACOM030955-RA">
        <property type="protein sequence ID" value="ACOM030955-PA"/>
        <property type="gene ID" value="ACOM030955"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON007827-RA">
        <property type="protein sequence ID" value="ACON007827-PA"/>
        <property type="gene ID" value="ACON007827"/>
    </dbReference>
    <dbReference type="GeneID" id="120960123"/>
    <dbReference type="CTD" id="33351"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_013026"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007827"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030873"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00187"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000075882">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000015">
        <property type="term" value="C:phosphopyruvate hydratase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004634">
        <property type="term" value="F:phosphopyruvate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03313">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.390.10:FF:000001">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.120:FF:000002">
        <property type="entry name" value="Enolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="entry name" value="Enolase-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="entry name" value="Enolase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00318">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000941">
        <property type="entry name" value="Enolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020810">
        <property type="entry name" value="Enolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020809">
        <property type="entry name" value="Enolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020811">
        <property type="entry name" value="Enolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01060">
        <property type="entry name" value="eno"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902:SF1">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00113">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03952">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001400">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00148">
        <property type="entry name" value="ENOLASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00002">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00178">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00001">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01192">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01193">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="Enolase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="Enolase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00164">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enolase N-terminal" evidence="14">
        <location>
            <begin position="3"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="Enolase C-terminal TIM barrel" evidence="13">
        <location>
            <begin position="142"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="10">
        <location>
            <position position="210"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="344"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="294"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="294"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="319"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="319"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="371"/>
            <end position="374"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="395"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001400-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR001400-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR001400-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007827-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="433" mass="46627" checksum="F4979A1F8C26F178" modified="2020-08-12" version="1">MPFKSIKARQIFDSRGNPTVEVDLVTDLGLFRAAVPSGASTGVHEALELRDNEKANWHGKGVLKAVENINKIIAPAVLSSNLCVTQQKELDELMLKLDGTENKSKLGANAILGVSLAICKAGAAKKGVPLYKHIADLAGNGNIILPVPAFNVINGGSHAGNKLAMQEFMILPTGASSFTEAMKIGSEVYHHLKNVIKAKFGLDATAVGDEGGFAPNILENKEALNLIQDAIAKAGYTGKVEIGMDVAASEFHKEGKYDLDFKNPKSDPSAWLTPDALEQMYQGFIKDFPIVSIEDPFDQDHWDAWSKITANTTIQIVGDDLTVTNPKRIATAVEKKACNCLLLKVNQIGSVTESINAHLLAKKNGWGTMVSHRSGETEDTFIADLVVGLSTGQIKTGAPCRSERLAKYNQILRIEEELGAGAKFAGKSFRHPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VTL6</accession>
    <name>A0A6E8VTL6_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Myosin tail domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_040219418.2">
        <property type="nucleotide sequence ID" value="XM_040363484.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VTL6"/>
    <dbReference type="EnsemblMetazoa" id="ACON007348-RB">
        <property type="protein sequence ID" value="ACON007348-PB"/>
        <property type="gene ID" value="ACON007348"/>
    </dbReference>
    <dbReference type="GeneID" id="120947782"/>
    <dbReference type="KEGG" id="acoz:120947782"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010114"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007348"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031970"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46292">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 102A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46292:SF1">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 102A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="137"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="499"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="31"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="213"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="499"/>
            <end position="514"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007348-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="520" mass="60367" checksum="16EE5B64D85D105B" modified="2020-08-12" version="1">MSQSAPRRHPPSGNVEVSSMSAKFVDTEWEARESQRQRELEEARARAAQMEKTMKWWSDCTANWREKWSKVRTERNKARDEAKQLRSNLEAAIKESNSYKREKCELEMQITQLKKEMEKVHTLMMKHAGRFNKASLDAADEPDRDGRGDNNCSPDISSDGLKNVNSEDGLVTKLPNLPDDGAGVPGAASANADLDIEEYILQGGAMPKHSVEFKDKSEQMAEERRLIQQLSKDDYDEDYLLQKITMLQLRLDDAQKTIQIEREEKNAVHRNLEKTRQDMQELRDKCEELRAAKQDAVRELLTLQEQHRVEMRITHNSLQEESIARETLERRLCELRTELERLQAENAAEWGKRERLETEKLNMERETKKLRAECQDLQERLERKGRPLVNSDVELRSLQQELLEKNKELSDIRHSHSKMKKMLSEANTELGHAVRRAEQYETEVKRLRSRVEELKHELASAEDELDAACNHVRRLQRTNEELSGQTEGLQVQIQHLQTRLRSSHGSTSLLSANEDNSDNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VTN1</accession>
    <name>A0A6E8VTN1_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Oxysterol-binding protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the OSBP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VTN1"/>
    <dbReference type="EnsemblMetazoa" id="ACON007687-RB">
        <property type="protein sequence ID" value="ACON007687-PB"/>
        <property type="gene ID" value="ACON007687"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002963"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007687"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037516"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097038">
        <property type="term" value="C:perinuclear endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.120:FF:000005">
        <property type="entry name" value="Oxysterol-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.3490:FF:000015">
        <property type="entry name" value="Oxysterol-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037239">
        <property type="entry name" value="OSBP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000648">
        <property type="entry name" value="Oxysterol-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018494">
        <property type="entry name" value="Oxysterol-bd_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972:SF209">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01237">
        <property type="entry name" value="Oxysterol_BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144000">
        <property type="entry name" value="Oxysterol-binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01013">
        <property type="entry name" value="OSBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="1" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="256"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="289"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="295"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007687-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="446" mass="51826" checksum="BFCC8A54C9C5DC46" modified="2020-08-12" version="1">MFSRNDFNIWSVLKNCIGKELSKITMPVVFNEPLSFLQRMTEYMEYAKLLRVASEQATPLERLKYVSAFAVSALASNWERLGKPFNPLLGETYELTRPDFRIICEQVSHHPPISAFHADSDSFRFHGSIHPKLKFWGKSVEIQPKGTVTVEFPKLNEAYSWSNVNCCVHNVIVGKLWIEQYGMMEVVSHQHGMKATLNFKPSGWNNKDLHRVEGYITDRNKRKTHFLYGKWTEFIKCTDYASYEEYVKENAHKFKRNADDRSKSPNESPGNTPRKVLSKLNSLKMSSFKSLSIQEPDDPPEPSEGDIPKSDSTYSIDIPNSVALWEAESRPANTAEYYQFTNFAMSLNQLEDHMKQPRALCPTDSRLRPDIRKLEQGDLDGAAAEKTRLEEKQRETRKKSKKGQEELVIFPRWFKQETNPHTKQEDWIYKGGYWERNDDNLELDIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VTN6</accession>
    <name>A0A6E8VTN6_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="6">SERPIN domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the serpin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VTN6"/>
    <dbReference type="EnsemblMetazoa" id="ACON007691-RB">
        <property type="protein sequence ID" value="ACON007691-PB"/>
        <property type="gene ID" value="ACON007691"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010250"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007691"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037348"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.497.10:FF:000062">
        <property type="entry name" value="AGAP007691-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.497.10">
        <property type="entry name" value="Antithrombin, subunit I, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023796">
        <property type="entry name" value="Serpin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000215">
        <property type="entry name" value="Serpin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036186">
        <property type="entry name" value="Serpin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042178">
        <property type="entry name" value="Serpin_sf_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461:SF211">
        <property type="entry name" value="GH10112P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461">
        <property type="entry name" value="SERINE PROTEASE INHIBITOR, SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00079">
        <property type="entry name" value="Serpin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00093">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56574">
        <property type="entry name" value="Serpins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0722">Serine protease inhibitor</keyword>
    <feature type="domain" description="Serpin" evidence="5">
        <location>
            <begin position="35"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007691-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="391" mass="43899" checksum="24C9E81B31619124" modified="2020-08-12" version="1">MVLFSVLLTSSVAIDVPPDPTTDDAIVAANNKFTLEYFKACYDEKCNCAVSPYHVRLALSMFYPLAGAAVQEDFQVAFGLPEDVHAAIEQQQRLAQQLHDGQHLKALSFVLVEETLRLDSEFERLFHRTFQTTVEPVDLTDDIPSALAVNSFYQRANTEIEDFIGEGDVFSLPPCHKLMLFSGVSVLTPLAIRFNPADTALELFQFINAPTQRVSTMHTTAFVRRCLHNELRCKVVDIPFDAASGLSMLVLLPYDGTELRQIVNSITPAHLAQIDERLQSCWTDLKLPKFFVREKTDPKQTLGKLGYGGVFEIDDLHVFHDSGRTRLNGFIQHCYLAVSESGSGIPAPPDTPSEFEFHGNRPFMFLIRRTMDGNVLQVGNFSKYIDPDEQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VTP1</accession>
    <name>A0A6E8VTP1_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="13">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the syntaxin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_049461312.1">
        <property type="nucleotide sequence ID" value="XM_049605355.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VTP1"/>
    <dbReference type="SMR" id="A0A6E8VTP1"/>
    <dbReference type="EnsemblMetazoa" id="ACON007698-RA">
        <property type="protein sequence ID" value="ACON007698-PA"/>
        <property type="gene ID" value="ACON007698"/>
    </dbReference>
    <dbReference type="GeneID" id="120951338"/>
    <dbReference type="CTD" id="42854"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001938"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007698"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034310"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:SNAP receptor activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:intracellular protein transport"/>
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        <property type="term" value="P:neurotransmitter transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:vesicle docking"/>
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        <property type="term" value="P:vesicle fusion"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Syntaxin 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Syntaxin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045242">
        <property type="entry name" value="Syntaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006012">
        <property type="entry name" value="Syntaxin/epimorphin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006011">
        <property type="entry name" value="Syntaxin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957:SF424">
        <property type="entry name" value="SYNTAXIN-1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05739">
        <property type="entry name" value="SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00804">
        <property type="entry name" value="Syntaxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00503">
        <property type="entry name" value="SynN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47661">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00914">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0532">Neurotransmitter transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="268"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="12">
        <location>
            <begin position="194"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="39"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007698-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="287" mass="33151" checksum="BA302B5CF1655980" modified="2020-08-12" version="1">MTKDRLAALQAAQSDDEDMPEDVAVPVEGSFMEDFFKEVEEIRMMIDKIQANVEEVKKKHSAILSAPQSDEKTKQELEDLMADIKKTANRVRGKLKGIEQNIEQEEQQSKSNADLRIRKTQHSALSRKFVEVMTEYNRTQTDYRERCKGRIQRQLEITGRATTNEELEEMLEQGNSAVFTQGIIMETQQAKQTLADIEARHADIIKLENSIRELHDMFMDMAMLVESQGEMVDRIEYHVENSRDYITTGQQDLVQAVKYMSKARKKKIMIAICLFVTVIILLLIFLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VTP3</accession>
    <name>A0A6E8VTP3_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="7">EF-hand domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON007888-RB">
        <property type="protein sequence ID" value="ACON007888-PB"/>
        <property type="gene ID" value="ACON007888"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012381"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007888"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038348"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034454">
        <property type="term" value="P:microtubule anchoring at centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18905">
        <property type="entry name" value="NINEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18905:SF13">
        <property type="entry name" value="NON-CENTROSOMAL MICROTUBULE ARRAY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="6"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="178"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="771"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="905"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1061"/>
            <end position="1080"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1389"/>
            <end position="1409"/>
        </location>
    </feature>
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        <location>
            <begin position="621"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="870"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="930"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="1012"/>
            <end position="1053"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="1246"/>
            <end position="1378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="54"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="114"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="179"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="217"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="261"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="773"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="789"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1391"/>
            <end position="1403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007888-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1409" mass="156831" checksum="04FD1102CAC2809C" modified="2020-08-12" version="1">MIIILYSDHPDQQSQQQQQQQQKQEVLDQQMASYASPVCLTTPGKANHIGGNAGSNSSTISSTSSNSSTSGSPSFHSAGGKFSNGGSSSNSGSTHKHRSPTQQRSNSAPKPFHGKTETSGHKLPHEDDEVVEKEKKNVSLSTVIGHAKTLPHDKQKTSVDGVPVDDNEFMSQPIVNRLPKHEAEVDETSDREVSPKFVVGTKKYGRRSRPREDVNGLDSSSLSSNSDNESVTVKSAARKTAAATAAEEEEPIPVHHVPGRTRQSFITGTGTVASSKVQRSASQSDIHGSKRRRPVMAGGTGVNRLKRCASLPTHRQRPTVVESKRNTTTVTIREQLHMDDDRIDQKIYFMNLSENLREIWDSLLLDSDRRHGDLLNQAQLEEVCERVGLQKVPARLAAQEVFNKLSLQPIEGIGFEEFIALLESNTDLLPMSETKELQLGDCGTGGSPTVTPSVVSPEESAFTLAMPPDWTSEIGSLAASIIIDMWESAGIDAPANLLHDLGFDRDVIQVADLVQALEEEHQRLVSGHMNSSTISNISSNPFDDAALIVRASLALHKAEVTALRQAFHQLVEENKKLYADNKEVNHRAVLLAQEVDERHNSLENSTRTKIRHLEQRHHETVKELSSQLAFEREQLSHANAMLEKRIQTLEGEEGKLKTEISRLAEENDELRQDQENLTKEITDLLEKNIKLNRDIAELEENNRNDYADDDECGFVRKDSEEVLDLIDRISLLQSENTGLRDKNDELSAEIETLNVELSKFKLKRCTTAAEDLSAAGSGDGPASSAAIKRRGDSPSKTRLADESHRLGKFRRCSNENDIESDSSSGDWMALHSELGQSLRGAEVAGCAELTGASSGISSMNESSLSRDDEIKTLRARIEELEVQLKEAKKEIALPEQVPAAEESANVAATSSEVAVPTPAENSTPTKEEECKKLKARCEELESSLEQMRKEYEDCEDYWQTKVNDERLLYEEEQRMSDEKFTELLKKVAEYEEQFAGNSKMEKDGRLSPIDEKDGLEQQYLELEADFEQAQLALEERTSEVEKLRRRIQEMEQMHKYPSEMLLSPSPRVETPDVEDRPASSPISYLWNQSTIQRPVRDYQNPNWRPPTVANAISDSILVEEKCSIAVATPLNAELVTENKSSEFMGSSTEVDDVASSSFDRIISPIQKPTTVVGSFDGESSSGAKEDESQHCTTVAVLDQVDASDACSVKSARSTHSLASTHSIQHSIANSEGSTSGVQTKHLRLMQLQLQDEIKDLTHERDCLMMELQQLKEAKSVLARSYAKTASHPNQMQKIQNLEQKNRHLQLMVKQQQQYTESILHQIWQQQRSEINDLRNRLEAQCVVISDQAARLANNDILVKDMYAENSQLMLQVQRLEHQCNRANWMQQYNQSNSSSGSHHGLSGIMPGLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VU00</accession>
    <name>A0A6E8VU00_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">CHK kinase-like domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
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            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
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        <property type="protein sequence ID" value="ACON007946-PB"/>
        <property type="gene ID" value="ACON007946"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="CHK_kinase-like"/>
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        <property type="entry name" value="EcKL"/>
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        <property type="entry name" value="Kinase-like_dom_sf"/>
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        <property type="entry name" value="CHK KINASE-LIKE DOMAIN-CONTAINING PROTEIN-RELATED"/>
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    <dbReference type="PANTHER" id="PTHR11012">
        <property type="entry name" value="PROTEIN KINASE-LIKE DOMAIN-CONTAINING"/>
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        <property type="entry name" value="EcKL"/>
        <property type="match status" value="2"/>
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    <dbReference type="SMART" id="SM00587">
        <property type="entry name" value="CHK"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CHK kinase-like" evidence="1">
        <location>
            <begin position="142"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007946-PB"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="488" mass="56385" checksum="7A46DE03981E7555" modified="2020-08-12" version="1">MDPQTIGLVENDIAESVKCYIQDKRNRVNGKFRLVSYQVRRLSEEPVGYLGDHYLLDVNLREKMVHYSPEEEEKYAEEEYISFFVKVIPDNVPKLADYIKEMGCFRKEILLYKHVIPRLQDVMIGTRPFVSTAYFTKGDRMIVFENLTSEGYSIVEHTKHLLDFEHLKVALQAVAKLHASSLILEERSKTPIPKLFVGYLNENAYVDDDDHVRKINLENAIQVLCEMIKRIEKYASSEKLDEILLKVPDVIRKIYDYSKPSTVFRNVLNHGDLWCNNVMFRYEMIPTSVDSSCINTEGHLPEGRKVTSDREDQAETGKDADVSNSALFVTNANHESRRRIPVEAKLVDFQLARFAPPVLDVWTLIMLCSTRAFRQQHLTALLDSYYQTLGDLLLSNSNMNIEPIISRNEYSASCDCFALAGLIESLLFSHLTLLPKQAAKDMLNCSENFDNFLRGNGKLHICLRYFEQDTNYRNRMTEMLEELVESLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VU04</accession>
    <name>A0A6E8VU04_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ig-like domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Myofibril</location>
        </subcellularLocation>
    </comment>
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        <property type="protein sequence ID" value="ACON007562-PB"/>
        <property type="gene ID" value="ACON007562"/>
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    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039286"/>
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        <property type="component" value="Unplaced"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045214">
        <property type="term" value="P:sarcomere organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="SPEC"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="AGAP007562-PA"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000519">
        <property type="entry name" value="Muscle M-line assembly protein unc-89"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000632">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
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        <property type="entry name" value="Uncharacterized protein, isoform B"/>
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        <property type="entry name" value="Uncharacterized protein, isoform B"/>
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        <property type="entry name" value="Ig_sub2"/>
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        <property type="entry name" value="Spectrin/alpha-actinin"/>
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        <property type="entry name" value="Spectrin_met"/>
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        <property type="entry name" value="IMMUNOGLOBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="9"/>
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        <property type="entry name" value="Spectrin_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="9"/>
    </dbReference>
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        <property type="entry name" value="IGc2"/>
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        <property type="match status" value="4"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1366"/>
            <end position="1462"/>
        </location>
    </feature>
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            <end position="1567"/>
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        </location>
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        </location>
    </feature>
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            <end position="2004"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
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            <end position="2337"/>
        </location>
    </feature>
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            <end position="3947"/>
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        <source>
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        <source>
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        <source>
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        </source>
    </evidence>
    <sequence length="3955" mass="438827" checksum="DB6D8AD546F302D2" modified="2020-08-12" version="1">MEQTPPGGDGQYSSLDELQRQLNEDFAQTLGPMTSPQQHMATASAAGAASSGASATIATTISTVVLQGGPLQLVVAILKSGEKLYIKVADLLPKFTFLGPSLDDALKLQNEHDELLRQIQNMPTPLEEFYRKIQEKIASNERPNPTLIEEMAASLNAVWHDIKQMLAERRDIVLLNVAFFERLGEAYGKMSSLEVACNDTMIPIEIDAVREFLDSFRDLRGDMLAAVAATLKAGQQMLDRLRVLSEIGTLDSRPNHIKLDAVQAIGQVESWLEDLSNRRNQLEQAWISRKTQLEQCLTLAILAKQLDEIEQCLARVRGQTLSSFTLGESAEQAAELLETYQNLKPEAALLRDKSLKITKTTEELLTSGCFAGDEACAKAYAVLSACSEHLEEIDQRESLLGQSRDFFTRAAAVLKRLDQLEQQVAGTPLRPASPNDLPAHQKLLQDIRETIGEVLQMGYSLVDDVGRTKPEVAGVQAMIERIEQRKLHFERYCNEESERNVRTAQALNEFLERYGQLFAWLEEAREQRLVRGDAIHRMGESLPEAKDCLLLHHQLLNDLEIKGNAINNLLVRLTPSLEHLADEQRDDVQRQIDLIRQHWIALKNCVIERVDLLKLYIRFHQEAETLRNQFDAHAIQFATFKDNPADHGLILAAIANIRQTLESLRPLGGQCLDQLHQIGDAYLQKTRAAQCVERTLAEFADRQSAVSNEWESWNSRRRTETTLKQIMAANMETLAVASKLEEQLYPLFSTALDNPADLIEFIGRKRTQLQQDVQAAEADLTQRFDTIAQLEAAGADPGTEQDMVAVKNNLNSIKTKLHTLGADFGELSDATEHFLRSILQCRDSIREYFGTKQTVTGPGSVEAIAEGYEQFKQATMEYFRGLLQQSEQIIERVKALEPPGARELDTDKIITLLENLRTYFESQTESENCELKKQHSVLAFDRELGEVRAAIRAEAERLERTRGQYGESVAEAQSVRVAFEERATILQDLERRIDAFISSGTELVRQYPETSPYIESETHKIRNEWTDLLRKLEEHRQLHTIAIEYFELITIIEEHYRTLNAELINANNKLTILNNADVANDLVAQVDNTIRTHETSQLDALKKIANLSTQLYGCDKTVTLYTENTKLFQTFYKIKTEVYERSKELGEEQARREEQQRQEQLKREQEEQQRQEQLKREQEELQRLAQLQREQEQEQQRLEQMQREQEQEQERLAQLQREQEQEQQRLEQLKREQEEEQLRLEQLKREQEQEQQRLEQLKQEQEQEQLRLEQLKREQEREQEQLQREKELAMQQQQEATIVEDVQITTTTSHAGLPHQQQSFDVQTIDEEQYLQEPQVPLVIQTVEEQVHEQIHEVVTTTSTLSTAQPEASLRFMQPLSDVCVQEGQRVRLQCVVAGVPDPMIEWYKNGISVQGNPDYRTSFDPASGVCTLMIDETVTADSADILCRAANEAGIADTTARLLVTEAPPPPPKPAGTAPTFTMRLLDGGVAQEGQPFQYDCVVTGQPTPTVHWYKGEQCVDGSPDYRQSYEPASGVASLRIEQVYLEDQTHYTCRAVNDCGSDETSAFLTVKPLEPTEFPNFPEPLSNVMARVGQKIRLEAKVSGTPQPELMWTHDGKHFTNREVKFYYENGRAQLVIDEAFLKDAGVYTLTAKNIAGEQCCSCNVVVKGRLPNETSDSELASDMEPVKPAVQLPLRDVSVFEGKPVRLDCVIVGQPEPEVIWYHGERPVKESNDFQLLFQGDRCSLVIREAFLEDAGEYRVVAINSAGEASSQCAVLVTPLNTAEPAVRQPVERVLPAVGAPPRFERLLSDILADEGERVQFECAVSGDPRPSIRWYVNNREIPTDVAVSPRVHCVVRDDGVVKLIIERALPDDKGVYTVKAANASGEAKCFSNLIVKTINAPEFETVPAFLTESLVCPTFRELFADRVVRQHEPTKFECIVIGKPQPKIRWFFNDQPVQGHDFLVSTSGDRQVLTIPEVRPELTGKITCYAENEAGNAQCVAIVQLLEQLGSVGPAPMATLPAATTESMQQIDTTGSSCVTLQKHVTTSSTTSSSFSSSSMVQQNGVAQSHAEVHAESAKLDRSFHQVGDQPAQVAEAAEHQRYDKVNDLPATVQHEASSYVGVAPSHETIIANSGQISTGKPARRNMAPRFVTPFNGKIVDQGADVIIEGIVDGYPTPDVRVTKNDVELQPDGERVKVSYSLNKIVVELRNVSPADAGRYTATASNAAGASTSTADLVVKKSIFPPVFGRRLQAQTVRNGDRVVLDVEVSGTPEPSVAWFKDGRPVQEAFAPGSYALQQVGPCFKLIFEQVSLNGTGTYMVLATNAGGEAQSTADIAVLQCESAAQPAPQPAPQKHVSFVDMVQQQQPRSEEALEGEGGGFSARQEYASEAKLHGPTTESTIVTETRRTTEATMRMEHKISFPELPPLRLSQRTQTPTVPTVTEGTSPMSAADPPSAGQQQQQQQQCAGTNTDPIATRSDATQTAPTTMTVDVPTAPSVRLPAQSESKRESSSISSSSNSVTSTVVRERPNSSFEPLLQQPADVGELTSAPLLVGSEPRPLSLLKPQPETIPTPPIGTLFQSVATEAPKFPLAPPGSSSVRKPRPASMSEVTRARSTSPRPSADAIAMERLWSTPKAFGSFGARAADEPPVCQVYRSYRPPPTVSSSSSTFTKHITTTTTQQQQQQQPPPPPPQHNYSTLPFARKQTKPHQEHNAAKAHSDSIRSDFQPIYQQPPQPQPQPVVNAPAPTQMYSSFSSTQQFQASSTLSQQQYSQSSMQYQPFKPSLAVEAPVRAPSPAPVPTFVPAAAAAPPPQSFPPKITPFKPALAPAAPVVAAAPAPMFAPAATQQPLNVGTSAPAPTCTLTPIAGATPAFVAGPSYNQTPQPFKPLPPPAITPSSLGSAAQPSVAAAAAAPLPTVSQPAYAAPQPQPQPQAFAEPTGPVSLPPYQEPLSFSSKPAVEPLYPGQLGGYQQPPQPQPPQQVQYQQQQVQQQQQQQQVYQQQQVYQQQQVYQQQQQVYQQQQQQQVYQQQQQSQQQYYMEQQDEEVYKKKSVKETKKLFEESLLQQQAAYGELKAPRMVQGVRLPVAAAAPLPADFGYGAIAELGLEPGPQPTMGYAPKASSERKSSYYREQIEQSLLESMDKEPERVPAGGVKIIPPSPRKKSKPAAPAGQSDTAGGVPAPLEQPAEPQQGQQGAKSPFTATESEYESDMDGVTGARRQNGYLADTEEVVQKTTAVSFEQQQQQQTVQTVESFSSSSKVESHSSVVSEVVQQQQQQQVVEQVEQKVEQVQEQQQQQQVTSEEQVQQPQEPQQPAQQSEEVIISSSETVQKETTVEESKSNFLVNNDLPEDLQPVKVLPTEEPLPTLKHVPVPAAVQPVPEPAPVPEPTPVEPAPQQLQQPLPQQPQPEPIAATPVQQNGYPTPSPAQPFLSDYESDAAAPSLAGYKPVHPVFAPPKQEFATGSAAPPPSVFDPIDKIQASQISAPADAPKIEKPKPISYVQQQQQQQQQYQSYQQQQTSSFQQQQTYQQQSYQSVQPPVQQSFPPAVQSMPAQYYTSVTGQPVTNTIETTNSLQMKEMTESSNRVLNMQQTKQTAYLQRPGRFTPGEYRDSDGYDSDGNRIRPLWTPNPSDSDEPHYRSVRPNFQQPRSTSLPRQYERIQTPMEFDTLPVQMPTKIELSAGGVESSHQRSSSLSRQSTEIQTTTTMTTETLKTQTLDRYASKRMATAPVSNSRDDMAVKAHRVAPINYIQKQADGMAQSFKTKAYHFNNEVMTDIKKTPIKPILKNAYGAPMPQQQQMAPPQQLQQQQGSAVPPQVYREESRVSQYDPTAQGFNRSTTSTPNVKHQEEGVPTKTPLFVAPLKDIAVVSGQPARFECIVACDAAPSIRWTKDEAPIEEGYKFVPEYRNGVCRLSLPVAYEGDAGRYCCLAENHLGYAATCAELTVANSDWRANQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VVR1</accession>
    <name>A0A6E8VVR1_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">CD109 antigen</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">TEP1-F</fullName>
        </alternativeName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="24">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Binds covalently through a thioester bond to the pathogen surface resulting in pathogen clearance.</text>
    </comment>
    <comment type="function">
        <text evidence="14">Modulates negatively TGFB1 signaling in keratinocytes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">Heterodimer of a TEP1-N chain and an TEP1-C chain non-covalently linked. Forms a complex composed of TEP1-N and TEP1-C heterodimer, LRIM1 and APL1C; the interaction stabilizes TEP1-N and TEP1-C heterodimer, prevents its binding to tissues while circulating in the hemolymph and protects the thioester bond from hydrolysis. Mature TEP1 and to a lesser extent full-length TEP1 interact with SPCLIP1; the interaction is induced by microbial infection.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Heterodimer; disulfide-linked. Interacts with TGFB1 and TGFBR1. Forms a heteromeric complex with TGFBR1, TGFBR2 and TGFBR3 in a ligand-independent manner.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.</text>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON008364-RF">
        <property type="protein sequence ID" value="ACON008364-PF"/>
        <property type="gene ID" value="ACON008364"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000438"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008364"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038887"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004866">
        <property type="term" value="F:endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02897">
        <property type="entry name" value="A2M_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.20:FF:000001">
        <property type="entry name" value="CD109 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1930:FF:000001">
        <property type="entry name" value="CD109 isoform 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000155">
        <property type="entry name" value="complement C3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.130.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1930">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.690">
        <property type="entry name" value="Alpha-macroglobulin, receptor-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009048">
        <property type="entry name" value="A-macroglobulin_rcpt-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036595">
        <property type="entry name" value="A-macroglobulin_rcpt-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050473">
        <property type="entry name" value="A2M/Complement_sys"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011625">
        <property type="entry name" value="A2M_N_BRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041813">
        <property type="entry name" value="A2M_TED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047565">
        <property type="entry name" value="Alpha-macroglob_thiol-ester_cl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011626">
        <property type="entry name" value="Alpha-macroglobulin_TED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001599">
        <property type="entry name" value="Macroglobln_a2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019742">
        <property type="entry name" value="MacrogloblnA2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002890">
        <property type="entry name" value="MG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041555">
        <property type="entry name" value="MG3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040839">
        <property type="entry name" value="MG4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11412:SF136">
        <property type="entry name" value="CD109 ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11412">
        <property type="entry name" value="MACROGLOBULIN / COMPLEMENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00207">
        <property type="entry name" value="A2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07703">
        <property type="entry name" value="A2M_BRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07677">
        <property type="entry name" value="A2M_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01835">
        <property type="entry name" value="MG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17791">
        <property type="entry name" value="MG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17789">
        <property type="entry name" value="MG4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07678">
        <property type="entry name" value="TED_complement"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01360">
        <property type="entry name" value="A2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01359">
        <property type="entry name" value="A2M_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01361">
        <property type="entry name" value="A2M_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01419">
        <property type="entry name" value="Thiol-ester_cl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49410">
        <property type="entry name" value="Alpha-macroglobulin receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00477">
        <property type="entry name" value="ALPHA_2_MACROGLOBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8" id="KW-0391">Immunity</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0882">Thioester bond</keyword>
    <feature type="domain" description="Alpha-2-macroglobulin bait region" evidence="21">
        <location>
            <begin position="457"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-2-macroglobulin" evidence="22">
        <location>
            <begin position="707"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-macroglobulin receptor-binding" evidence="23">
        <location>
            <begin position="1324"/>
            <end position="1413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1435"/>
            <end position="1457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="1443"/>
            <end position="1457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00056820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00057615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00063008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00063781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00069665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00078071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="SMART" id="SM01359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="SMART" id="SM01360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="SMART" id="SM01361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008364-PF"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1481" mass="164699" checksum="02630449AE673C9E" modified="2020-08-12" version="1">MRRSTMFASKKDPAIRILSTSSSSLLAVCLVLSVVLVPAVQCEGHYSIVGAKLLRPNSEYHVAVTNQDVSEPIRFSLAITDASSVIAKQEITLNTGETRLVPFAIGDIPESSYKLVAEGLSGLTFKNETDLEYQQKSFSVFVQTDKSIYKPGDTVRFRVLVLDPNTKPLQKADNISVHINDAKANRIKQWKEGKLVKGVFESELTLSTAPVLGAWTINVEVLGSKHNKVFEVDEYVLPKFEVTVESPGITTFKDGKVKAIIRSKYTYGKPVKGEATVSVSPEFQFNYVQPFAKDVITRKVIPIDGKGSVEFDLREDIHLEGDYSRNIVIEAVVEEELTGRKQNASAKVMIYDRRYKMELVKSDDNFKPGLPYTAWLKVSYQDGAPVQDQTNPVEVKQSSFESTTSVQNYTLDQNGMAKLEINTEVNSSYINVVGVYLGQEFYLHGISKAESDVDSYIRAQVLTEMPLVGKDVLVEVTSTSPMKYFTYQLLGRGDVLLSNTIAVPESKTQSFKFPATFAMVPRAKLVVYYIASNGDMVSDSKVITFDSELQNFMKVSLSKEQSKPGQDVEISISTNPDSYVGLLGVDQSVLLLKSGNDITKQQVFSELEKYEERSYGFYRRKKRFAWNPHAEHRDFSTVGAFVMSNANDPPQKHYYLEDMFFLESAVSAGIDVPRPEMISFDRASDAIPQVAMPAEAPVTIRRDFVETWLWLSSVSFSGEKTLQKKVPDTITSWIITGFSVNPIYGLGLTQQPRKLNVFLPFFVSTNLPYSVKRGEVVAIPIVVFNYMEDDQTAEVVLHNDEQEFEFADVENEVVESNKVELFRQKRLDIASNTGKSVSFMVKPKKLGHITIKVTAKTKIAGDAVERQLLVEPEGLPQFINKAAFIDLRAAPELTKTFEVEIPKNAVPDSTRIEVAVIGDVMGSTIQNLDSLIRMPYGCGEQNMLNFVPNIVVLDYLKATNKLTANIEAKAKKFMEAGYQRELGYKHRDGSFSAFGENDKSGSTWLTAFVARSFKQAANHITIDEGVIDKSLEWLSDHQAPNGSFPEVGVVSHKDMQGGSGSGVALTAYTLIAFLENINLVDKYKNTINKAIDYVYRNTESLDDTYALALAAYALQLADHSSKGLILSKLDTKATTDSDSKWWHKPIPETEQKNPWYSRPNSVNVEMSAYGMLAFLEAGLDTDALPIMKWLIGQRNDKGGFQSTQDTVVGLQALAKLAAKITSPNNDVTLTAKINENQEKRMTVNAENGMILQKFELPSAARNIEIQATGSGFAVVQLSYKYNMNVTGEWPRFVLDPQVNANTNPDYLHLSVCASFVPSAGQNVSNMAVMEVGFPSGFTADSDTLPSLENMPFIKKVETKDGDTTVVLYFDSLDQRELCPTISAFRTHKVAKQKPAPVVIYDYYDNSRIARQFYDGPKASLCDICENEDCGEACSIRSQKQRSSDSPSRQPTVEGTMQSGSQTVSVSFFTFLLATLLVRMFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VVR2</accession>
    <name>A0A6E8VVR2_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VVR2"/>
    <dbReference type="EnsemblMetazoa" id="ACON008014-RD">
        <property type="protein sequence ID" value="ACON008014-PD"/>
        <property type="gene ID" value="ACON008014"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001942"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008014"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_036081"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026272583" evidence="1">
        <location>
            <begin position="16"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008014-PD"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="239" mass="24173" checksum="0D19C008D644F785" modified="2020-08-12" version="1">MKFLVLCTLVAGAAAGYSASTYRDGKATGYNGGYTTHFSGPNGAGVAVGTGTGSFAPGFAGFPGPTVDFNNFFQGIQANFANLYQQQFALQQALFQQQQAAFNSAAFGGPGFGGAGFTGAGAGTGFGGFPVYGANFASAPNYIQTRFPAGNFNGASSSASFGPGGFHQTAAVYPGNPAVPNVDTRFGGSEQATSFQSGKPGFVGVSSFSSSSNINGQTHREAVTTVNDNGKVTTHRVHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VVR8</accession>
    <name>A0A6E8VVR8_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Bromodomain adjacent to zinc finger domain protein 2B</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WAL family.</text>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON008017-RB">
        <property type="protein sequence ID" value="ACON008017-PB"/>
        <property type="gene ID" value="ACON008017"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000763"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008017"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041439"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05503">
        <property type="entry name" value="Bromo_BAZ2A_B_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01397">
        <property type="entry name" value="HAT_MBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15545">
        <property type="entry name" value="PHD_BAZ2A_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15489">
        <property type="entry name" value="PHD_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000199">
        <property type="entry name" value="Bromodomain adjacent to zinc finger domain 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.890.10">
        <property type="entry name" value="Methyl-cpg-binding Protein 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037374">
        <property type="entry name" value="BAZ2A/B_Bromo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018501">
        <property type="entry name" value="DDT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001739">
        <property type="entry name" value="Methyl_CpG_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028941">
        <property type="entry name" value="WHIM2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45915:SF2">
        <property type="entry name" value="TOUTATIS, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45915">
        <property type="entry name" value="TRANSCRIPTION INTERMEDIARY FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02791">
        <property type="entry name" value="DDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01429">
        <property type="entry name" value="MBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15613">
        <property type="entry name" value="WSD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00571">
        <property type="entry name" value="DDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00391">
        <property type="entry name" value="MBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50827">
        <property type="entry name" value="DDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50982">
        <property type="entry name" value="MBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MBD" evidence="17">
        <location>
            <begin position="1055"/>
            <end position="1129"/>
        </location>
    </feature>
    <feature type="domain" description="DDT" evidence="16">
        <location>
            <begin position="1367"/>
            <end position="1430"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="15">
        <location>
            <begin position="2990"/>
            <end position="3040"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="15">
        <location>
            <begin position="3044"/>
            <end position="3096"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="14">
        <location>
            <begin position="3468"/>
            <end position="3538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="175"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="287"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="554"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="714"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="784"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="877"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1023"/>
            <end position="1054"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1196"/>
            <end position="1263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1664"/>
            <end position="1863"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1907"/>
            <end position="1967"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1981"/>
            <end position="2011"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="2105"/>
            <end position="2441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="2476"/>
            <end position="2509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="3097"/>
            <end position="3227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="3253"/>
            <end position="3448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="16"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="175"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="220"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="296"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="311"/>
            <end position="353"/>
        </location>
    </feature>
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    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="468"/>
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    <feature type="compositionally biased region" description="Gly residues" evidence="13">
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            <begin position="579"/>
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        </source>
    </evidence>
    <evidence type="ECO:0008006" key="20">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="3560" mass="378944" checksum="313C613E685F5C47" modified="2020-08-12" version="1">MDKDGEKKGGGDKKGGGGQSGTGLGGAAGVNDPAALLDAASLFAYWGRDPSAMAAAVSNPLFGSQFGMPGGLGGLMPNAGSGSGSGNDRFAMSHHNQNTMAVAASQAASLAGLHNNWWSMAQLAAQDYFARLQATGMSQLPFSHDLAGAFPAGLGLGAMSAAAAAAAVAATGGNAAGGGAGGSGSGTGGSGGGSGKGGSGGGKGKKRDRSSNSNSSNASSGGGGSAGGMGGMGAGGMGGGVGGSNNSSYKNSPSPSASQAAAAAAYKQSLYSQATLHKELMAITAAAAAAQNQQHSGGGSGGGGGGGGGSSSSQQSQQQQQQHQQQQQQQQQQLQQQQLQQHQQHQLASLGMLAGLGGTGSSGSASPGSSSSKQKSSSSGGGGSSSSLASPHGASRLIGNDSISITRGSSSPSMSSSKQQPTPSVTISASHGNPGSSSSSSSGSSAQQNHLQNLSSSSRSSKDGKGGNSNNANASGGPSAADLGLSASMNALSTLSQFNNLDLTTQQNMTATMNALAASQAKAKDYISSGILNDPSSLLGVRLPPDTEIIKYTSSIVGPKASGGTGRGPKRQRLEPNEYGGGGGGGGSNNSGGGGGGGSGSSHLSLASGSGGSAGLLPPGLSHHHHHQLGSNGGLGSGASSGTGGSNDRIEVIKLPPTITSNGVYNAGKGAGKDSMMDNMNEWSGLNLSAKGSGGGGGSSSAAATIVSSLTAGGMNSSGSNNQSSGMMEQDDAPLNLSMKPSKSSNEGMRSDSTHSASSSSPAMSGASANSLQSLSTITAALGGAAGNANDSSRSRRKSDNKNRRNAAAAAAAAAAAAAAALGAGPLGLDGSVNMSLASAAAAYAAAAPVSTGAGLGTGTNSTSTLNSLLMASVGTGTGTNTITTTSNTTTSGGGSSGSGSGAGIAGGSSSAAGGASSSATAAANAANHQLMAAQLGFNSSLSELLKISNYEEYDYASLSGGSQFKEGRPRNLGRGVSKPKKNTVASLLAQSRAVGSKPLTAQQLLTQDAEIEKLRQAMLEASRNQSMDNSTSNTNTDTESISESGMSESEGEEHINLKELRVPLEKGWRRETVIRGLTRNGHIKGDVYYYPPQSVNKMKGMNQIQLYLDQFKPKDLSRDNFSFSAKAIVGTFLQPAPLPYATDGEFIKMTDVEVARRLEDLKMFTRHAGLGVEQRIEIAKQQQALRDAKKLAKEEMNKNKEKARQAKEAERNERLEQQRKERELKNQQALEAKKKREEELARQKAEEAARKAQEKEQKRQQALLQKEQELAKQKELMYAMEMERERRRQHMALIKQLELRRKFEEKEKKKHQVILDKLIQREKKLVMRKRDTNILAELRKPQEDSEIVDQTVLPSFSRIPGLKLTGTGYADLLMVFEFLHNFGETLGFDMESLPNLQSLHLALTSENAIDAEEELLSVMTHLLVCAIEDPGIPNPGRHTTLLGQTLRQADITHTNVSEILRIYLYAVATGEVKLQSGINLERDRDAPSKHHLVNDEDFKMCSTKNSQFYELLAENARYKLSELLKDKPFVALNPTTKTEILAMLCNDLLMNKAVCKQIDSSLEAQAQLKKERYLLDNKIRKYKMLVARKQRLEQYEKAQQAALEKSLSMKAAEEAKRAEEAAAMAAAAAAAEAAAAAAAEAAASAATASSEEAALAAEGETKLPAGETEATAGDSECEVVENGTEKESAPPVEGEGFNSVPPAEGASGQGELEEGEIVNKKDFGAEGHKTDDDDDDDDNDVVEIVPMPVTCTVTEDSCPNTIPTSSEVEGEENGQSKLDTTAAPESGEPMDTSSVEPSESSSLNQNHTESLNGIDVPTPAGDGCSTRIEGNGPSAHHPLPEEHHAPPTTPPPPPPPPPSQHAIVHPVMQQHKAIMQLEPGEIPPPGMDISALGMRHMAGGEIQTISVPSTPEESPVKMGEVGGETPNGGVFNIPGKPLTAEMTNGGEQPSQHQQQTPQVHQNQSNNSASELDLLSKSMINDHSTCGDRADEDNSDLESEGTQLEEDEDAHLTAEEAQRKYDKILETSFQNKQQLENALNQLRVKCFGQDRYWRRYWNLGKCGGIYVEAMESTQPEMYRYENALEEVQSRPDYVPKYAPAIKPESAVKQDPAEDVAAECKKEEGEVDAVKPEIIADAQGTKQELQPTEDRKRKRCSSVSLKKLKKKKKKQRTTSEGGESFRGNHGSAAGGFNNAADDEDDGDDEEEDGPDDEDDCSRTSFSNGPALEDGRTADSQSDDCKIIEEPEVVQKEGASNGENGANGQSATREGTGEDRTGSSSEINNGSNGNADGEVHGQKENEAQQENHKSAEDGQSVEGTPNHNATPKKSDDQLMDIEDSIPTAILVQKGNDHDETKTVEVNNQIGGVNVSPHQPTAGSGASAATPEDDDDVTMVQEETPTITIPDDDTESGDRMPTAPCEGVKTEHDNKGSTTLTDSMNCDMKPKLMENGVEMRDPGELVECQLQEVDDDEEIVTGARGNHGSANDCKPMIAAGDNGGVDRKPRTTTTTSSVISYGSDVEMIEVKDEDSNNCAVRAPITPGSYLYLRNTDTKQEKEFSDQLLNRWFSIVDKELPLSSTECPLPTINGSTPASLARQIFTNITCREICQIQGNRWDIGNNIQFFSVPLEKGVEIHFPNESILSMSGLDDDEINEVIAKKSRPEPLQLSDMKPAFKRETIEYSYSQHHPNQIRLRAEMMAEETADPEEYGNFSLPAYMTLTLSNLTAYVQCDQFQPLQMTPEEEKQLEDVKQHGAPQKTEPQVVPREFRYGWWKINDIEELNELIKALNPRGVRERLLRQSLLESLAESVNLTTPHHVSHPRAAPPPNGYIEPEAWNAWNPSIARRVEVALLDQIEAMEDKVASASMQVKGWQMPQREGDSENGVVEDVTIEMLRERILGLEAAIERRYLKPPLGINTTEAQMAVIAQQESHSNQNNVSNLSNCSNSSAEDENLPKGLLSWRDAVERSVTTAQLSMALYVLESCVAWDKSIMKANCQFCQSGESEDKLLLCDGCDRGYHTYCFKPRMDKIPDGDWYCFECKNKATGDRKCIVCGGLRPPPLGKMVYCELCPRAYHQDCYIPPMLKYPRGKWYCQNCVAKAPPKKKPQRKPKERTTNNSSQSLLNSSLNSSQNQSLNSSHEDIATTPLSPAHSIASTSHEESSAPQHLQPPHSSVPGVPMPAAPATASTSSAYHHQLQQQQQHPQQPLPPPLHPQQYGGAPLVDANNYAMCHPPVPNETMALYGQQQAQQQQQQQYYQQYYQQQQPSTSVGPALAPPAPPQQPYYPTPVESSATSAATVEQEAQSDYVASGEAAQYSASEGYTHAQSTSECEPDQQESPAEVDDQIEASSESPVPAAGGGEFYSPSSNAMNAAGSSTSYLDSDRTPGGQDGEEGSCGGDSSEEYQPRPSPVKDSLCLAGSSSSSSSSISDHQRKERSKERDEAKERAKQEKKATKRLLKELAVCKTILEEMELHEDSWPFLLPVNTKQFPTYRKVIKSPMDLSTIKKRLQDLVYKSREDFIADVRQIFDNCEVFNEDDSPVGIAGHGMRKFFEQRWADLTDKHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VVT0</accession>
    <name>A0A6E8VVT0_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040239530.2">
        <property type="nucleotide sequence ID" value="XM_040383596.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VVT0"/>
    <dbReference type="EnsemblMetazoa" id="ACON008631-RB">
        <property type="protein sequence ID" value="ACON008631-PB"/>
        <property type="gene ID" value="ACON008631"/>
    </dbReference>
    <dbReference type="GeneID" id="120959875"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006946"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008631"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033661"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004307">
        <property type="term" value="F:ethanolaminephosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006646">
        <property type="term" value="P:phosphatidylethanolamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1760:FF:000016">
        <property type="entry name" value="ethanolaminephosphotransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048254">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014472">
        <property type="entry name" value="CHOPT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10414">
        <property type="entry name" value="ETHANOLAMINEPHOSPHOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10414:SF36">
        <property type="entry name" value="GH11618P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015665">
        <property type="entry name" value="CHOPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="47"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="88"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="182"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="221"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="260"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="288"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="319"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="350"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008631-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="395" mass="45302" checksum="E4D99035122D94B0" modified="2020-08-12" version="1">MIGIKYLNDAHLKGFEKYKYNCVDTSILSVYVMHPFWNKVVLFCPRWIAPNLLTFTGFLLTVVNFFLIAYYDYDFRAATETPIPIPDWVWILAAINLFVAYTLDGIDGKQARRTGTSGPLGELFDHGLDSYSAVLIPIYMFTIFGAADLPPVRMFFITLNVFMNFYLPHVEKYLTGVMFLPWGYDFVMWGVSITLAITGIFGAEFWQIPVFGMKPCHIFELTLYISAVITSHPIIIYNVYKSYRDKTGKMRSFREAIRPLVPLSSLFILCTLWVLCSRNGIIDMEPRLYFVMCGTLFSNICCRLIVAQMSDTRADLWNGLLNLLCVVTLFSVLPYPSLGLPELSIEIERYLLYGLTVCVTIAHLHYGAGVVREMCHHFRIRCFKIAPHDKPLLSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VVT5</accession>
    <name>A0A6E8VVT5_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="13">SH3 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the calcium channel beta subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VVT5"/>
    <dbReference type="EnsemblMetazoa" id="ACON008028-RA">
        <property type="protein sequence ID" value="ACON008028-PA"/>
        <property type="gene ID" value="ACON008028"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011326"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008028"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039976"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005891">
        <property type="term" value="C:voltage-gated calcium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005245">
        <property type="term" value="F:voltage-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11863">
        <property type="entry name" value="SH3_CACNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000023">
        <property type="entry name" value="Voltage-dependent L-type calcium channel subunit beta-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046937">
        <property type="entry name" value="CAB1-4_N_A-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000584">
        <property type="entry name" value="VDCC_L_bsu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11824">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12052">
        <property type="entry name" value="VGCC_beta4Aa_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01626">
        <property type="entry name" value="LCACHANNELB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00072">
        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="9" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="domain" description="SH3" evidence="12">
        <location>
            <begin position="66"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="147"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="409"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="45"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="147"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="409"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008028-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="452" mass="50282" checksum="79C795B3187045AA" modified="2020-08-12" version="1">MLRHKNHHKRHNTTYKPPFMRKRRGSADSNYSQPSSDLSLDEEKESLRREKERQALGQLEKARSKPVAFAVRTNVSYDGSLDDDSPVHGSAVSFEVGDFLHIKEKYDNNWWIGRLVKEGCEVGFIPSPVKLEHIRMQASAARSSKLYTSKGSSSSGNLGASGVPGAEPSRGSTPPTPDDSDSMGASRHGKTPLATPPTKEKRKPFFKKQETSSPYDVVPSMRPVVLVGPSLKGYEVTDMMQKALFDFLKHRFESRIIITRVQADISLAKRSLMNNPSKRAIMERSNSRSSCLAEVQAEIERIFELARTLQLVVLDCDTINHPSQLAKTSLAPTIVYLKIASSKVLQRLIKSRGKAQAKNLSVQMVAAEKLSQCPPEMFDVILDENQLEEACNHLAEYLEAYWRATHPPVRPTPSVPRPLPSQEASPSGEQPGRMGPPPPTVFNMTDVEDTLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VVT6</accession>
    <name>A0A6E8VVT6_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Peptidase S1 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S1 family. CLIP subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VVT6"/>
    <dbReference type="EnsemblMetazoa" id="ACON008091-RA">
        <property type="protein sequence ID" value="ACON008091-PA"/>
        <property type="gene ID" value="ACON008091"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_013705"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008091"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037375"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000068">
        <property type="entry name" value="transmembrane protease serine 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040973">
        <property type="entry name" value="CLIP_SPH_Scar"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252">
        <property type="entry name" value="ACROSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252:SF7">
        <property type="entry name" value="HYALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18399">
        <property type="entry name" value="CLIP_SPH_Scar"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039934975" evidence="4">
        <location>
            <begin position="21"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="5">
        <location>
            <begin position="461"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="215"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="327"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="235"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="340"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="364"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008091-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="734" mass="79641" checksum="2E52A7DB5437D288" modified="2023-06-28" version="2">MNHRTRALVGLLCIAGLAMGQLNQSYRPEFFSPGQGFQSVSAQISSNNYGGTTVTKTQERTDGGITSKVITTGTITRPYFKQTTLTSDGYNYPVPETIPCYEPSKICAPNQYCNDGFVDESQLNLFNTNNQNCNSATEQCCKIRPTPATRCPDDSYVCVSPSLCNNGLLNFDAQNAISTRQPTGECYAPEVCCKQQSLLSQSTVLTNEGYVVKDEIPNPANDTPVVIRPTTQRPFQPPRPQGPEAPPPLPPVGCSAAMNCTEEEYCSSTGVISKTPVVLTEEQKLFRVPVTPCRNLERGFTGVCCRDPDYVDPWPVGQLGQYNPEILGFDDGSYKPTNGNGNGNAVGNGNGNGNGRRPGEPIQASPSQVTSTQSFAPNQFVSQTASVTQSKSQYDRGVTASASFQSTVGQRVNAAPAGLQFTNSGNTRFRPFQNQRSESTFAKPQQCAPRNYNTQPRGAGPLGTGFGEFPWQAMVLLETNKSLLCGGAIISDNTVVTAANCVYGLNPRTIQIKGGEWRLGVDAEPKTFQIVRVKDIVYHPAYNPTTLNYDVAMLVLEDRLKFDTHIGSICLDENDVVPSASYENCVTTGWGKEVLKIHIQNALMQQMSISLLSEAESQSQLQRNGFAPESHICGRPSGDACEVDVGSALACADTSGTYYLKGVYSADNGCNRPDQIVSFSNIDVQWIKQALKNPNQFITPVPNYQTAANSPQVSRVQLQSTQGPAYNNKYLPPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VVT8</accession>
    <name>A0A6E8VVT8_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MaoC-like domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_040232704.1">
        <property type="nucleotide sequence ID" value="XM_040376770.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VVT8"/>
    <dbReference type="EnsemblMetazoa" id="ACON008667-RA">
        <property type="protein sequence ID" value="ACON008667-PA"/>
        <property type="gene ID" value="ACON008667"/>
    </dbReference>
    <dbReference type="GeneID" id="120955687"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007513"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008667"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_036877"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1050.10:FF:000001">
        <property type="entry name" value="Putative Non-specific lipid-transfer protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1050.10">
        <property type="entry name" value="SCP2 sterol-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003033">
        <property type="entry name" value="SCP2_sterol-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036527">
        <property type="entry name" value="SCP2_sterol-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10094:SF25">
        <property type="entry name" value="SCP2 STEROL-BINDING DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10094">
        <property type="entry name" value="STEROL CARRIER PROTEIN 2 SCP-2 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02036">
        <property type="entry name" value="SCP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55718">
        <property type="entry name" value="SCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCP2" evidence="1">
        <location>
            <begin position="16"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008667-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="120" mass="12720" checksum="E0CE84BECB50B3DD" modified="2020-08-12" version="1">MTAAVDLQSDAVFAGIKDRVAENEAKAKAINAVFLYKITSGGKVAKEWVLDLKNAKVYEGPVQGGGKADTTMTIADGDMIELALGKLQPQTAFMKGKLKIAGNIMLAQKLAPLLKTEAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VW60</accession>
    <name>A0A6E8VW60_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="6">FUN14 domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FUN14 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VW60"/>
    <dbReference type="EnsemblMetazoa" id="ACON008503-RA">
        <property type="protein sequence ID" value="ACON008503-PA"/>
        <property type="gene ID" value="ACON008503"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000080"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008503"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032144"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007014">
        <property type="entry name" value="FUN14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21346">
        <property type="entry name" value="FUN14 DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21346:SF0">
        <property type="entry name" value="RE45833P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04930">
        <property type="entry name" value="FUN14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008503-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="139" mass="14622" checksum="8E32BC027833B933" modified="2020-08-12" version="1">MSDGKVKKDATNKEIINMSDAAGYLDRFVGDVSKKSATKQILIGAGSGWATGYITMKVGKMAAIAVGGGIILLQMANQQGIISIDWNKVQKKVDKVTDKVEEAVTGQGPSWADKVLKVAKENSFLATGFLGGFLIGIAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VXV3</accession>
    <name>A0A6E8VXV3_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Calponin-homology (CH) domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Nav/unc-53 family.</text>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON009424-RB">
        <property type="protein sequence ID" value="ACON009424-PB"/>
        <property type="gene ID" value="ACON009424"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002403"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON009424"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035529"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022008">
        <property type="term" value="P:neurogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001111">
        <property type="entry name" value="neuron navigator 2 isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057568">
        <property type="entry name" value="CortBP2_NAV1-like_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039041">
        <property type="entry name" value="Nav/unc-53"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057126">
        <property type="entry name" value="NAV1-like_ubiquitin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12784:SF28">
        <property type="entry name" value="PROTEIN SICKIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12784">
        <property type="entry name" value="STEERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25408">
        <property type="entry name" value="AAA_lid_NAV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23092">
        <property type="entry name" value="Ubiquitin_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="5">
        <location>
            <begin position="1550"/>
            <end position="1710"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="109"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="240"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="397"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="616"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="861"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1024"/>
            <end position="1198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1287"/>
            <end position="1347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1406"/>
            <end position="1434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1854"/>
            <end position="1876"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="1250"/>
            <end position="1277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="40"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="138"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="205"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="248"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="295"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="321"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="397"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="448"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="501"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="525"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="543"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="582"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="616"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="633"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="898"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="1029"/>
            <end position="1039"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1045"/>
            <end position="1056"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1065"/>
            <end position="1080"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1093"/>
            <end position="1106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1123"/>
            <end position="1140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1141"/>
            <end position="1154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="1179"/>
            <end position="1192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="1333"/>
            <end position="1345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1863"/>
            <end position="1876"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009424-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1876" mass="200077" checksum="2C10E9CF28ADA6F9" modified="2020-08-12" version="1">MGNSSSSSSSRNEKGRKCRSLPNSPDVRRYCSTFSPSSSLRRETTRPEVQPRRQRPVMSYRAMLLTLATGPDGQTQLPVPYAKAGVNGGTAIPLPATVLVTRRCPPDKVRPLPPTPNQQTGLHGGLQKHTLGAGGIGNSTPSDGNSCPGSPQHHNGTAIITQIPKGNNSLRLPQATNCKIPASPYSGSSTLQHHHQHHNNNNNNTITLIPNGNVTNIPATNNPSAQKHSMLDKFKLFNSKEKQDRAAQKSQISKRTSSSSGFSSARSERSDSSLSLDNGHHQGSVQPSSAIPGAKIKESGLKKLEGGVKSPSPAPGSTKPSSNSTAKLISSKSSSKDGSSAKAAAKAAAAAEKQRHRDAPPLEQSPPQTHIPLAPQKAQKIPMATTVTTTSLATVASAMAKPSPDPSSSSSRLKLAAVGGSRKLDAKSESKTSLQLIGAPKPTPMVPPQQQQQQAQQMQQPAVGTGIPKPMAAIKGTSKPSSQPPSVVTGKEELTVCKPQQVPNNNNANGGAISLSGNNNTMMGQLLQQQQQQQQQQQQQLLTNGASNGLSDSMHSNSTQGASIGQHSTNSSETSSVVAYRPSSESGSEQLLQSPQKLHPANGVAAAGPLVFNGQPDVNLNSNGTASPGHVFHNGNLHHSSSSSSNNNNHSSNTANNNNNLNKFHTVPAKLLNTSAAGLQSPGGHQTTIYEQEEKQITVLPMRPLLRGYNSHVTLPTRGTRGHHSHHPHHHHHQQQLMAADYCEDFAGGGYCSDGDALRKVPARYSDNMDNGYLSEGGGGGGSVSMLTSAGRQQHASYISSLRARTQLPTTIEERSTKSEKMYPSMLSRGPDVEIEPYYCLPVGTVVHPGNGGMVPWSQPTSPTPPTRGFGGLLSPTHAGNSAGSRLTYPKKNDDVHGSQASLLSGGSSLYGSTEERQAGEVRRLKRELADARDQVMSLSSQLSTNAHVVAAFEQSLSNMTHRLQSLTATTEKKDSEILDMRQTIELLRKQSIQAGLTTAHMQSMGVQVNGQVNGTAIAAEQTKTTTTTGGGGGSGATNGGQKSTGGASSAAPAGAEMQRHHSSDSMCSLNSVSSGCSAQDKSKQKKKGWLRSSFTKAFSRNAKISKTNRHVTAGGSNGGGSTTTTAAAAAGTVPDGAAGNHHHHHHHHHHHHGSVLENGTTTTTTTTSALGLKHAAGIPPPPGAGLPPPSPTKGSPHKTMTVIDNAKPVDAIVELNGNPVVEDLKKQLREKDLVLTDIRLEALSSASQLESLKDTVMKMRQEMMNLKQNNERLQRLVTTRSLAGSEVSLGPVSPSGSIGEPRRYSLAADNGMGRAPLELPQNLDETEEDNIPPAPAPEPPPAPLSPAIIAELSPTIERAAMVNEILTTPAEDVADPVDGKKISIAVYLGQPESFPKYAEEMNECDNYYHSSPDDEEKGEAGEKGGHPRGGSFSLGSGVETNEFVIAYTYISGKTTWQNLDYIVRKTFKDYLSRIDPGTNLGLNTDSITSYHLGEAKRGPEMGFPELLPCGYIIGNVKTLYICLQGVGSLAFDSLILRNIVHRYISLLTEHRRLILCGPSGTGKSYLARKLAEFLVARAGRDNPIEAIATFNVDHKSSKELQQYLGHIAEQASMSNGAAEELPAVIILDNLHHASALGDVFSCLLSATAAKLPCIIGTMSQATCNTTNLQLHHNFRWVLTANHMEPVKGFLGRFLRRKLFSVELQNQQQQPQLEKVLKWLPTVWQHINSFLETHSSSDVTIGPRLFLSCPMNLPDSQVWFTDVWNYHLAPYLMDAVREGVQLYGRRGGAWVDPCTYIRETYPWPVGPTTVPQLRQITADDVGLEASAPGNAENQDPLLNMLMRLQEAANYNGNAEQDSDCASLDSNMTHDSSAGAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VXW1</accession>
    <name>A0A6E8VXW1_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cytohesin 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VXW1"/>
    <dbReference type="EnsemblMetazoa" id="ACON008737-RB">
        <property type="protein sequence ID" value="ACON008737-PB"/>
        <property type="gene ID" value="ACON008737"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007605"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008737"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033609"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032012">
        <property type="term" value="P:regulation of ARF protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01252">
        <property type="entry name" value="PH_GRP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00171">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1000.11:FF:000002">
        <property type="entry name" value="Cytohesin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.20:FF:000003">
        <property type="entry name" value="Cytohesin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000309">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1000.11">
        <property type="entry name" value="Arf Nucleotide-binding Site Opener,domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023394">
        <property type="entry name" value="Sec7_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000904">
        <property type="entry name" value="Sec7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035999">
        <property type="entry name" value="Sec7_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663">
        <property type="entry name" value="GUANYL-NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663:SF402">
        <property type="entry name" value="MIP16918P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01369">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00222">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48425">
        <property type="entry name" value="Sec7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50190">
        <property type="entry name" value="SEC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SEC7" evidence="3">
        <location>
            <begin position="70"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="2">
        <location>
            <begin position="275"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008737-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="409" mass="47697" checksum="1CE8FB013A960127" modified="2020-08-12" version="1">MVNTMESSYATMSNISQHTENFPLPELTPEQQKLLIELRRKKQEILLEIQQINDELNEVESEMKAHESKEENKHSNKDKQMALGRKKFNMDPKKGIEFLYENQLLRTDPQDVAQFLYKGEGLNKTAIGDYLGEKNDFNEQVLKAFVELHDFTNLILVQALRQFLWSFRLPGEAQKIDRMMECFAQRYCQLNPDIFTNTDTCYVLSFAIIMLNTSLHNPSVKEKPTVEQFISMNRGINNGGDLPRELLESLYESIRAEPFKIPQDDGNDLMHTFFNPDKEGWLWKQGGRYKSWKRRWFILNDNCLYYFEYTTDKEPRGIIPLENIAVREVTDRSKPHCFELHASGGADIIKACKTDSEGKVVEGKHTVYRMSAATEEEQQEWITRLNQSISHNPFHDILVQRKKKALAKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VXW9</accession>
    <name>A0A6E8VXW9_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ABC transporter domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VXW9"/>
    <dbReference type="EnsemblMetazoa" id="ACON009466-RC">
        <property type="protein sequence ID" value="ACON009466-PC"/>
        <property type="gene ID" value="ACON009466"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011440"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON009466"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034839"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050352">
        <property type="entry name" value="ABCG_transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041:SF133">
        <property type="entry name" value="GH24286P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="265"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="295"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="335"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="374"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="403"/>
            <end position="421"/>
        </location>
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        <location>
            <begin position="433"/>
            <end position="453"/>
        </location>
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        <location>
            <begin position="484"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="74"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005814"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009466-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="514" mass="57795" checksum="EA1BB8C07F20F11B" modified="2020-08-12" version="1">MAEESYEMNPASSAELNGGPADPEAGTSPVSGVGGRRAGAAKLEPLQIPAVPAVEQEIAAPSLIAARLFKPVTVSFKNLSYSVRNGIFRKGKTRRDILKDISGEFRAGELTAIMGPSGAGKSTLLDILAGFLEIATHDYGRLNDSLVEKMMNGQSNFYRNPEPFSPTFESKLLPIKESSPDGPMVLLEMPLFPEHDNGDARNQQRPVSPVLATPKKLAKKLSFNPERWCGRDEIYTTSFCRQFSLLLLRTFLILSRDRSLMTMRFAIHCLIAPLIGMLYFGIGNQAAQIFNNYNYVFFSIMFLMFTAFSSMTMAFPLELPIITREHFNRWYSLRAYYIAMTVADIPIQLLCTVTYILITYYMTGQPPEAFRVGLFTLICLMVAWVAQGLGLLVASLFDVKNGAIFGPFFICPFLIFSGFFIHLTDAHPIMHWLFHISFLKYALQGAALAIFGYDRPRMDCDETYCHFVLPKKFLKEVDMLEADFVEAVIALGVIFFVFRVSAFYVMQYRMKNKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VXY3</accession>
    <name>A0A6E8VXY3_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="9">BZIP domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bZIP family. ATF subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VXY3"/>
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        <property type="protein sequence ID" value="ACON008762-PA"/>
        <property type="gene ID" value="ACON008762"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_013826"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008762"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_036218"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039250">
        <property type="entry name" value="CREBL2/REPTOR-BP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21051">
        <property type="entry name" value="CAMP-RESPONSIVE ELEMENT-BINDING PROTEIN-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21051:SF4">
        <property type="entry name" value="CAMP-RESPONSIVE ELEMENT-BINDING PROTEIN-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07716">
        <property type="entry name" value="bZIP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="8">
        <location>
            <begin position="4"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008762-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="76" mass="9067" checksum="47A944D428614018" modified="2020-08-12" version="1">MKAKLERSRQSARECRARKKLRYQYLEELVTDREKAVVELRRELEKLYNWALEVDAGRCPDGLQELLEELGAMKQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VY70</accession>
    <name>A0A6E8VY70_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Sphingomyelin synthase-like domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sphingomyelin synthase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040231593.2">
        <property type="nucleotide sequence ID" value="XM_040375659.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VY70"/>
    <dbReference type="EnsemblMetazoa" id="ACON008926-RA">
        <property type="protein sequence ID" value="ACON008926-PA"/>
        <property type="gene ID" value="ACON008926"/>
    </dbReference>
    <dbReference type="GeneID" id="120955126"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010676"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008926"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034322"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047493">
        <property type="term" value="F:ceramide cholinephosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033188">
        <property type="term" value="F:sphingomyelin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006686">
        <property type="term" value="P:sphingomyelin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045221">
        <property type="entry name" value="Sphingomyelin_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025749">
        <property type="entry name" value="Sphingomyelin_synth-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21290:SF27">
        <property type="entry name" value="PHOSPHATIDYLCHOLINE:CERAMIDE CHOLINEPHOSPHOTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21290">
        <property type="entry name" value="SPHINGOMYELIN SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14360">
        <property type="entry name" value="PAP2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="95"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="141"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="175"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="240"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="269"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="295"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="Sphingomyelin synthase-like" evidence="11">
        <location>
            <begin position="237"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="28"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF14360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008926-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="371" mass="42025" checksum="31E856DFF37CCB01" modified="2020-08-12" version="1">MEINARGSHKNGSGLDLATVVMEVPDSYDYNSPNAPNHHPSAYPHPGSPYHQQNHQPPPSDTGSPSSDAVMIKIDTPSPLRDDQSRYPKEIGKTLLALLFLAGNFFLATVSLSLVHDRVPDRDVYGPLPDAFLDRVQPQDWALDVSEILIMVVVNSCVLLITFHKHRFIVMRRVFLLLSILYMMRSLTMYVTVLPVSSRTYYCSPKSNASSAGVIVKRAFQLISGMGLSINGKQIYCGDYIYSGHTVTLVLGYLVISEYSPKRFWPIHWIYWLASLTGVVMVLVAHGHYTVDVLIAYYATTRLFWTYHTLANNSLLLKQNGNNYIGREWWYFAFRYFERNVRGPIPLQYDCPLSLPSSWTKKSPKLPGRES</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VY73</accession>
    <name>A0A6E8VY73_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="4">C2 NT-type domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
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        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <strain evidence="4">Ngousso</strain>
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        <text evidence="1">Belongs to the EEIG family.</text>
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        <property type="gene ID" value="ACON009577"/>
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            <end position="154"/>
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            <begin position="322"/>
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            <begin position="350"/>
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        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
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</entry>
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    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">Ngousso</strain>
        </source>
    </reference>
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        <property type="gene ID" value="ACON010291"/>
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    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010291-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="89" mass="10000" checksum="0FA1D9A3897BCF29" modified="2020-08-12" version="1">MVSNITRENVQKTTKYPSLVDLDVVSHPCSTRTSSTAIFYYDGARISSEHNYVDKNDRLTLPAPYNPFIGPKTSKELNINSTFLTNDSI</sequence>
</entry>
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    <name>A0A6E8W1F1_ANOCL</name>
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        <recommendedName>
            <fullName evidence="3">Serrate RNA effector molecule homolog</fullName>
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        <alternativeName>
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        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
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            <taxon>Neoptera</taxon>
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    </organism>
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        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
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                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ARS2 family.</text>
    </comment>
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    </dbReference>
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        <property type="gene ID" value="ACON010382"/>
    </dbReference>
    <dbReference type="GeneID" id="120955382"/>
    <dbReference type="KEGG" id="acoz:120955382"/>
    <dbReference type="CTD" id="35539"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009491"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON010382"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033879"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016604">
        <property type="term" value="C:nuclear body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031053">
        <property type="term" value="P:primary miRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039727">
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    </dbReference>
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        <property type="entry name" value="SERRATE/Ars2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021933">
        <property type="entry name" value="SERRATE/Ars2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13165">
        <property type="entry name" value="ARSENITE-RESISTANCE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13165:SF0">
        <property type="entry name" value="SERRATE RNA EFFECTOR MOLECULE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0943">RNA-mediated gene silencing</keyword>
    <feature type="domain" description="SERRATE/Ars2 N-terminal" evidence="9">
        <location>
            <begin position="144"/>
            <end position="253"/>
        </location>
    </feature>
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        <location>
            <begin position="758"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="296"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="645"/>
            <end position="669"/>
        </location>
    </feature>
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        <location>
            <begin position="868"/>
            <end position="893"/>
        </location>
    </feature>
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        <location>
            <begin position="8"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
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            <begin position="32"/>
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        </location>
    </feature>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="319"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="380"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="414"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="433"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="447"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="460"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="486"/>
            <end position="513"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00005407"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00017364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00030701"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF04959"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF12066"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010382-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="967" mass="109597" checksum="07B6F9B213C31E09" modified="2020-08-12" version="1">MGDSDDEYDRKRRDKFRGERSAGGGGDSYGRGADRPDRSRGRDDWPDRVRPRQDYRDYRPPPRDRGYSPAREGPTVKRMRGDTWGDEGRHRFGAHDGYGMYGYAHDHFGMHPTVGPYGHQHATHQREPVVSGDMLTQPCMMTLKQFLATQDDSISDSDAITKYNEYKLEFRRQQMNEFFVAHKDEEWFKIKYHPEESQKRKEEQLSFLKRRCEVFLELLKSKEIGKVSVDASNTDALLRLLDTVVIKLEGGTEEDLKVLDIKPSVVEAAARTPATLNEEKVKTESKVDATAKELAVKMEEDSSKQETETDVLSVPQSEEDARNGNSKIKEELSKSMDDGNENGDKSKSNDAQKSEKEIAEVEHEAHKHESEDRKKRSRSDSGSSSSSSSSSSSSESEEEKDERMNKEDNEDEENMRIPEEEKKSDSADTMDNINRDKEVDEGKPVCDAEDLNTADDRDDNADQNKEQNEFEETTKDKNSSDPVNDVNKENSDKKSQQEGDSKAETIDLAKDPADSGSRALHRTSSIFLRNLAPSITKAEVEAMCRRYNGFLRVAIADPLLERRWFRRGWVTFKREVNIKEICWNLNNIRLRDCELGAIVNKDLSRRVRPVNGITCHKTVVRSDIKLGAKIAHNLDDKWGLWKETPTAASGTGEAGDKNGSEVQPEESFGLQSKNPVLQNITDYLIEEASAEEEELLGLSEDSKKVSEGELIERDPQLIEVLDRLILYLRIVHSVDFYNHCEYPYEDEMPNRCGIIHARGPPSQSKVMSNEIQEYIRTFEGKMASFLTRMVDIDETDMKKLGAKDAEAEVEKFITANTQELAKDKWLCPLSGKKFKGPDFVRKHIFNKHAEKVEEVRKEVEYFNNYLKDSKRPQLPEHPGNAKKPGSEGATSATANASVGAGGYRNQPFGMSHSYAPMYASYAAASMMAPNPRGRAGFGRGGRMGGPDYRPVIHYRDLDAPREPDEFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W1F8</accession>
    <name>A0A6E8W1F8_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Spectrin beta chain</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Probably plays an important role in neuronal membrane skeleton.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the spectrin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040232501.1">
        <property type="nucleotide sequence ID" value="XM_040376567.2"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON010895-RA">
        <property type="protein sequence ID" value="ACON010895-PA"/>
        <property type="gene ID" value="ACON010895"/>
    </dbReference>
    <dbReference type="GeneID" id="120955574"/>
    <dbReference type="CTD" id="32746"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_013935"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON010895"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033256"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045169">
        <property type="term" value="C:fusome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016328">
        <property type="term" value="C:lateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031594">
        <property type="term" value="C:neuromuscular junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008091">
        <property type="term" value="C:spectrin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045170">
        <property type="term" value="C:spectrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051693">
        <property type="term" value="P:actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016199">
        <property type="term" value="P:axon midline choice point recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042062">
        <property type="term" value="P:long-term strengthening of neuromuscular junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048790">
        <property type="term" value="P:maintenance of presynaptic active zone structure"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007026">
        <property type="term" value="P:negative regulation of microtubule depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007274">
        <property type="term" value="P:neuromuscular synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd21246">
        <property type="entry name" value="CH_SPTB-like_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21248">
        <property type="entry name" value="CH_SPTB_like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10571">
        <property type="entry name" value="PH_beta_spectrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00176">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000020">
        <property type="entry name" value="Spectrin alpha chain, non-erythrocytic 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000003">
        <property type="entry name" value="Spectrin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000004">
        <property type="entry name" value="Spectrin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000011">
        <property type="entry name" value="Spectrin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000018">
        <property type="entry name" value="Spectrin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000019">
        <property type="entry name" value="Spectrin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000028">
        <property type="entry name" value="Spectrin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000033">
        <property type="entry name" value="Spectrin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000059">
        <property type="entry name" value="Spectrin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000083">
        <property type="entry name" value="Spectrin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000106">
        <property type="entry name" value="Spectrin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000024">
        <property type="entry name" value="Spectrin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001589">
        <property type="entry name" value="Actinin_actin-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041681">
        <property type="entry name" value="PH_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001605">
        <property type="entry name" value="PH_dom-spectrin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018159">
        <property type="entry name" value="Spectrin/alpha-actinin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016343">
        <property type="entry name" value="Spectrin_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002017">
        <property type="entry name" value="Spectrin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11915">
        <property type="entry name" value="SPECTRIN/FILAMIN RELATED CYTOSKELETAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15410">
        <property type="entry name" value="PH_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00435">
        <property type="entry name" value="Spectrin"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002297">
        <property type="entry name" value="Spectrin_beta_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00683">
        <property type="entry name" value="SPECTRINPH"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00150">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46966">
        <property type="entry name" value="Spectrin repeat"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00019">
        <property type="entry name" value="ACTININ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00020">
        <property type="entry name" value="ACTININ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3 11" id="KW-0117">Actin capping</keyword>
    <keyword evidence="8 11" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="15">
        <location>
            <begin position="50"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="15">
        <location>
            <begin position="169"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="14">
        <location>
            <begin position="2175"/>
            <end position="2287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="2091"/>
            <end position="2193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="2295"/>
            <end position="2319"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="776"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="988"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="1316"/>
            <end position="1343"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="1628"/>
            <end position="1662"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="1854"/>
            <end position="1881"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="2091"/>
            <end position="2105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="2106"/>
            <end position="2130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="2182"/>
            <end position="2192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00054264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR002297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010895-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="2319" mass="268574" checksum="356C702D6C7434D8" modified="2020-08-12" version="1">MTTDISVVRWDPSQGPGNEFIEDIEYDGGNSSSRLFERSRIKALAEERESVQKKTFTKWVNSHLVRVNSPIKDLYVDMRDGKNLIKLLEVLSGERLPRPTKGKMRIHCLENVDKALQFLREQRVHLENIGSHDIVDGNASLNLGLIWTIILRFQIQDITIEETDNKETKSAKDALLLWCQMKTAGYHNVNVRNFTTSWRDGLAFNAIIHKHRPDLIQFDKLSKTNPIQNLNNAFNVAEEKLGLTKLLDAEDIFVDHPDEKSIITYVVTYYHYFSKLKQETVQGKRIGKVVGIAMDNDRMINEYESLTSELLKWIEVTIVQLGDRHFVNSLVGVQQQLAQFSNYRTVEKPPKFVEKGNLEVLLFTLQSKMRANNQKPYTPKEGKMISDINKAWERLEKAEHERELALREELIRQEKLEQLAARFNRKATMRETWLSENQRLVSTDNFGFDLAAVEAAAKKHEAIETDIFAYEERVQAVVAVCNELEAEKYHDIERIAARKDNVLRLWNYLIELLRARRMRLEFSIQLQQNFQEMIYILDSMEEIKQRLLTDDYGKHLMGVEDLLQKHSLVEADINVLGDRVKQVVQNSQKFLVDEEDNYKPCDPSIIVDRVQRLEDAYAELCKLAVERRSRLEENRKLWQFYWDMADEENWIKEKEQIVSADEIGHDLTTVNLLLSKHKALESEIQSHEPQLMAVSEVGDELVRRGHFGADRIDERLKEILAMWSNLRELTEYRRKRLEDAVDYFQLFADADDIDNWMLDALRLVSSEDVGRDEANVQSLLKKHKDVADELKNYAETIEQLHAQAKRLTLNEPEQERVNERLAAIDNRYRELMELAKLRKQRLLDALSLYKLISESDGVEQWIGEKERMLQTMVPGKDIEDVEIMKHRYDGFDKEMNANASRVAVVNQLARQLLHVEHPNSPEIIERQNHLNNSWSKLREQAESKRDDLKSAHGVQTFYIECRETVSWIEDKKRILTETDSLQMDLTGVMTLQRRLSGMERDLAAIQAKLTALENEADAIEGDHPEEAALIRERVATIQTIWEQLTQMLKERDSKLEEAGDLHRFLRDLDHFQAWLTKTQTDVASEDTPTSLPEAEKLLNQHQSIREEIDNYTEDYKKMMDYGEGLTSEPTQTEDPQYMFLRERLKALKDGWEELHQMWENRQVLLSQGLDQQLFNRDARQAEVLLSQQEHVLSKDDTPVNLEQAENQLKRHEAFLTTMEANDEKFNTIVQVAGQMTSKDHFDADKITKRAESIAHRRDDNRNRALELHEKLKNQVKLHEFLQDIEELTEWVQEKYITAQDDTYRSAKTVHSKWTRHQAFEAEIAANKERLHEAKKAAQELMVEKPEFKEIIEPKLTDLSKNFDELETSTKEKGALLFDAKREVIVQQSVDDIDSWMDDLEKQIINTDTGNDLTSVNILMQKQQIIQTQMAVKARQVEELDKQTEVLTKTAPSDVLEPIVEKKTAVNARFEKIKAPLLERQRQLEKKKEAFQFRRDVEDEKLWIDEKMPLAESTEYGNSLFNVHVLKKKHQSLNTEIDNHEPRIMTICNNGQKLIDEGHEDAGSYADLISQLTQKWQELKDAVENRHRQLDQSEKVQQYFFDAAEAESWMSEQELYMMVEDRGKDETSAQNLMKKHESLEQSVEDYADTIRQLGETARQLTTEQHAYSDQVSVKQSQLDKLYAGLKDLAGERRARLDEALQLFMLSREVDDLEQWITDREVVAGSHELGQDYDHITLLWERFNEFAQDTATVGSERVAKANGIADDLIHAGHSDSATIAEWKDGLNESWQDLLELIETRKAMLAASRELHKFFHDCKDVLGRINEKQHGVSEELGRDAGVVSALQRKHQNFIQDLMTLHSQVQQIQEESAKLQAAYAGEKAREITNREHEVLNAWAHLQSMCEERRGKLADTGDLFKFFNMVRTLMLWMEDVVRQMNTSEKPRDVSGVELLMNNHQSLKAEIDTREDNFSACLALGKELLSRNHYASADIKDRLLQLTNSRNALLHRWEERWENLQLILEVYQFARDAAVAEAWLIAQEPYLMSTELGHTIDEVENLIKKHEAFEKSAAAQEERFSALERLTTFELKEMKRRQDAAEEAERQRLEAEAAAARAAAEAEAEAIRQQEAAAARDAADAPGSPHSTREQESVTMRATSPVEKERPISQTDRPSPGGADEGAQEGILTRKHEWESTTKKASNRSWDKVYCVARSGRLTFFKDQRSAKSVPEQTFRGEPPLELKGAQIEIASDYTKKKHVFRIKLSNGGEFLLQCHDDAEMQQWVTALKAQCELDASGEGRSLTLPASSQKDEQKRRSFFTLKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W1I6</accession>
    <name>A0A6E8W1I6_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Helicase ATP-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">Ngousso</strain>
        </source>
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        <property type="gene ID" value="ACON010872"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:helicase activity"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="P-loop_NTPase"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF136">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DHX35-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010872-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="90" mass="10155" checksum="69D16C7DC058751D" modified="2020-08-12" version="1">MSASRFPKFLKPDDESFASERDAAPADDHVTSFVFNAHQSLNLAAQRERLPIRQYRDQILYCLEHYQTLVLVGETGSGKSTQVPQFVAVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W5N7</accession>
    <name>A0A6E8W5N7_ANOCL</name>
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        <submittedName>
            <fullName evidence="6">ANK_REP_REGION domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">Ngousso</strain>
        </source>
    </reference>
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        <property type="gene ID" value="ACON012141"/>
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    <dbReference type="VEuPathDB" id="VectorBase:ACON012141"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038750"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
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        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011646">
        <property type="entry name" value="KAP_P-loop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052771">
        <property type="entry name" value="Neurotrophin_sig_adaptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057092">
        <property type="entry name" value="SAM_KIDINS220"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24116">
        <property type="entry name" value="KINASE D-INTERACTING SUBSTRATE OF 220 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24116:SF0">
        <property type="entry name" value="KINASE D-INTERACTING SUBSTRATE OF 220 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07693">
        <property type="entry name" value="KAP_NTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23307">
        <property type="entry name" value="SAM_KIDINS220"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
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        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="527"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="552"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="686"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="718"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="64"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="97"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="130"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="197"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="230"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="263"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="296"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="329"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="362"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="KAP NTPase" evidence="4">
        <location>
            <begin position="470"/>
            <end position="998"/>
        </location>
    </feature>
    <feature type="domain" description="Kinase D-interacting substrate of 220 kDa-like SAM" evidence="5">
        <location>
            <begin position="1261"/>
            <end position="1337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="895"/>
            <end position="914"/>
        </location>
    </feature>
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        <location>
            <begin position="1204"/>
            <end position="1253"/>
        </location>
    </feature>
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        <location>
            <begin position="1362"/>
            <end position="1383"/>
        </location>
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        <location>
            <begin position="1464"/>
            <end position="1515"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1533"/>
            <end position="1556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="895"/>
            <end position="905"/>
        </location>
    </feature>
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        <location>
            <begin position="1225"/>
            <end position="1251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1369"/>
            <end position="1382"/>
        </location>
    </feature>
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        <location>
            <begin position="1475"/>
            <end position="1493"/>
        </location>
    </feature>
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        <location>
            <begin position="1494"/>
            <end position="1504"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07693"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF23307"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblMetazoa" id="ACON012141-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1556" mass="169611" checksum="7D4BCEDEF7318B60" modified="2020-08-12" version="1">MFKSKHRSAGHEHHHHHQQALNRCDSMGSLGHRSLLQYLETDDLAGLKSFLGTRHLQVDDRDENNTTVLMVASGRGATHFVKELLARGADVQAQDLDSWTALHFAAKAGHVGIVELLLDNGAELEHRDMGGWTALMWGSYKGHTSVVALLLQRGADVQAHGNYHLNPLLWASGRGHTEIVRLLVNTGGAKVNVGDKYGTTPLVWACRKGSAEIVDVLLKAGANVDTAGMYSWTPLLVAVSGGFQECVSLLLERKPNVNALDKDGMTALSIACREGLTEIASALIAAGAYLNVQDRAGDTPLINAVKGGHRSVVEILMKRHVDVDIQGKDKKTALYTAVEKGHTAIVKLILQSNPDLELSTKDGDTALLRAVRNRNLEIVQMLLERKAKVGATDKRGDTCLHVAMRARSKAIVEALLSNPKYGQLLYRSNKEGETPYAIDATHQKTILGQVFGNRRLNANEDSEGMLGYGLYSSALADVLSEPTLTTPITVGLYAKWGSGKSFLLTELRDEMKNFAHSWSEPPIDASWLFFLISLHLVLVIGTVVGLATWSYVWGMVTGATLLVLIYFTDILLKFLDRRYDLEWLYSLNYGLSRKLGRLRLILQVAFCHPPGPQSDQQPMPVRFHFAEASGAAPNGDAAVALMLASLFDAIEAHYGSLATGLYRAFRPKPLKATGGWKWRKMCCMPVVLMFELGMLGILATASLSIVYSEYGLEGREEIAVAIYVLLGILLAGAIANLHAWSKLIGALFMSQGKHLKRAFNSNEAAPLTALGAEVSLMTDMVRCLDAFTGQQSRLVGVVDALDSCDTERTLTILNAVQTLLSGPQRPFVLLLAVDPHVIAKAAEANSRRLFTEGGIGGHDFLRNLVHLPVYLQNSGLRKVQRAQNTAMSAYRRAMPDLSRDDDQPHLGHSASARRLSNASEIMSSQEKLRAMPSSSRGGSKKLRVSDSIASSIGSNLHKLGQNPPVDLSKIILTDDYFSDVNPRSMRRLMNVIYITVRLLKAFQIDFSWYRLSSWINLTEQWPLRASMIVLEHDQGGDSFDDSVSLQAVYDKVRPKLTCLREAAALLDLDRDERKLDAFLQLHKSDLLVSDLRIFLPFTINLDPYLRKVLKEDQQALEDEGIIIPMKTVLPPSKPSGFVPHHHHRAPVGGVLQPTPQHPNNLTNWPNFYNPQPQAMALMSYYNQNWANFPAGVYGTNALLGEPLPKPGSVGGGPIITEHPPHEQHSTLGSNSGRSSKTTTGALQKSSSNGLATSPPIDIDLSNVQLSSLTVEQLIELLEKVNDLKPAMERTAPILRENAISGRVLMYCNLEELKSVLRLSFGHWEMFKLLVTALREASVTQPASRKLSTKTTSFAKGTNDSVEMQEPIAQSTPPFGSSTSSFQPIRQKSQNLLEKQVTLEEQMICGALQTLNEDAFEDVVSSSERPSPTGEMFSQYLAPIRESSEIGSPPRLTYNLTNPNLTDLAASNGTLNGDDSGVGGGGSVGVGGGGGGSTSSGSHLGLRSGSGRHSRSHSLHDDASLTSIVVIPTFLTTSPNGASSSNNNNNNTTTNINDTKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W5Q9</accession>
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        <submittedName>
            <fullName evidence="2">Calponin-homology (CH) domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
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            <taxon>Diptera</taxon>
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            <taxon>Culicidae</taxon>
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            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8W5Q9"/>
    <dbReference type="EnsemblMetazoa" id="ACON012181-RB">
        <property type="protein sequence ID" value="ACON012181-PB"/>
        <property type="gene ID" value="ACON012181"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012974"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON012181"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037706"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
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        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="114"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="177"/>
            <end position="210"/>
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        </location>
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            <begin position="579"/>
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        </location>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
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            <dbReference type="EnsemblMetazoa" id="ACON012181-PB"/>
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    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
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    <sequence length="1030" mass="116049" checksum="0125A0FB1F8F6842" modified="2020-08-12" version="1">METITLLRSSNSTAKSQDALHSSQLPPEKPHLTKGVTVENIVKRLSSERHTSPPPSQIVGGGFSYTRGSGEQPIVYAQVVANETNKSKHTVKQQHYPSTEEEDPATMWTAGESKLRPSGGFLDTTQRPSPPLPKYRLDEADHSPLTINGIRRKLHTNSDEDEGLGIDYARQKQAQERYKSSAYVHTSSSTTRTSPKSFLTNSDEDPPIAPSIVRLTPTEKNFSSSYSVHYRGQADGKEHLPEFHELSQRREILESRIRSRIGSRDLLDRMSPERTVPVPIQLTSGGHRTSNSSSTTKYRTTTERRSVDRVLDRHESPVRRSTLSPTPTYREASQERGAVPRRNSRGYVETFVTKTVVNRDGKPVSEEYVERYKYPGESVEAVTSSRTVYDREGSAGRTEVQRYNRYVPEQQGGTSSYTTRHTVDKGDSGIENDFRKDSFNHEFISGTKFTLTENVRACEDFLRMERTHTNSCMRKPRKAHVYRERSIDDGSRYDPRLDEISASRSYTRDSTLKSSLKADKKAGGLAKVKQFVSSTRKKLVRMGTDTSEKRDHSRVGSLRSDSADGSRVPDIATRRRLSTPKSSPSSAKRSLSFKSSKLSSTTPAEGQKQSSSRSEWFKSFDRLSRKKSSSKPNLTVDAKSPPPPPKQAKSLRFFGDTDTEYGDQNATSYRSARSNLSKTEKKYRSAYDLDTAPKVRDTARNRSSSLHNLDEEDELSLRGKDYRSEEHSSTFYRARSPSPSDYRPERTGTPRDYTPERVTRKAYHHHHRSRENNIDLVDGERSQTPVHQRPGSRSRSEHKKPPSGPQKPARTFERRDSLNRDIDRILDSSGTEGESSQQSQRSVVFLHATTVGDIPHPQIANARRRAYSRESLNHSVASSKKVQPMTRTVSRSISVLAPWKPKNIREGYEIDYHKDQHQAKPISTLPRSGATSRQSTVGSQSTLSRQPKHRSTPSARDDPDSDKSSSFEQSRRHSADQQQQQRRASGSRETGGLATPYASTTLPSSRRVKDSIAQYPSRTAGSGSTLKYRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W614</accession>
    <name>A0A6E8W614_ANOCL</name>
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        <recommendedName>
            <fullName evidence="9">LIM zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
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            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
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        <property type="gene ID" value="ACON012305"/>
    </dbReference>
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    <dbReference type="CTD" id="37853"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005913"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON012305"/>
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    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
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    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
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    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 8" id="KW-0862">Zinc</keyword>
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            <begin position="9"/>
            <end position="69"/>
        </location>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblMetazoa" id="ACON012305-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="94" mass="10133" checksum="081FE4F12F199310" modified="2020-08-12" version="1">MPFKPADNPKCPKCGKSVYAAEERVAGGNKWHKQCFKCGMCGKMLDSTNCAEHENELYCKNCHGRKYGPKGYGFGGGAGCLSMDTGAQFQGEGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W7S7</accession>
    <name>A0A6E8W7S7_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8W7S7"/>
    <dbReference type="EnsemblMetazoa" id="ACON013481-RC">
        <property type="protein sequence ID" value="ACON013481-PC"/>
        <property type="gene ID" value="ACON013481"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009241"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON013481"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030170"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON013481-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="213" mass="24689" checksum="B6D916127AE54E6C" modified="2020-08-12" version="1">MVYESDFYSTRRVGSSYTRPTISSYTVTTPLRYSGVPREFEDAKSDIRNSTALLLRQLNDPVPRLMAPIAQAAPEPNPKKWVYDPFSTHNRLNSDTYVKSHITDPIRSVRNDIEAMARYHSPASRYVGKNHLASTRIIGSQAYPKTKPRIYNYDTAKVGKDVNVMSYYKSNRTQAKADVEEVNTKIKKYLEKYYLNHAYKVPDEFKEFLHEYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W7U5</accession>
    <name>A0A6E8W7U5_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PBP/GOBP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8W7U5"/>
    <dbReference type="EnsemblMetazoa" id="ACON012323-RB">
        <property type="protein sequence ID" value="ACON012323-PB"/>
        <property type="gene ID" value="ACON012323"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011149"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON012323"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033278"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="term" value="P:sensory perception of smell"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="PBP_GOBP"/>
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        <property type="entry name" value="Pheromone/general odorant binding protein domain"/>
        <property type="match status" value="1"/>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="4 6" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
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    <feature type="chain" id="PRO_5026187202" evidence="6">
        <location>
            <begin position="22"/>
            <end position="120"/>
        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="120" mass="13926" checksum="9414EC8CCFF299DE" modified="2020-08-12" version="1">MGRLDLVCLLAIVLLVHSCVSIVWKPSVFESLRAGNFSVRNSLVECFGECFVKRAGFMNDNFTFNRDTIMRFTNRFVSKEISEKVYNICTDNVTPTYCVTAFDVYQCIYENVYKSWDSRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W811</accession>
    <name>A0A6E8W811_ANOCL</name>
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        <submittedName>
            <fullName evidence="4">Methyltransferase FkbM domain-containing protein</fullName>
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        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8W811"/>
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        <property type="gene ID" value="ACON013730"/>
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    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001889"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON013730"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030434"/>
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    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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            <end position="139"/>
        </location>
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            <end position="383"/>
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        </source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="610" mass="68294" checksum="CAFFCFD2ECAA2B8A" modified="2020-08-12" version="1">MRTKVQEKKGKMSTDEQQQQQQQQNQIPNIAGTILIGGIGGGLDGGQHKQQQQQQQHQAQSQHNPHQQQQHHLHSYSHILPHLHQFSTMTGSNPASLTGGSKLPAKSSPLGPSPFRQLLPVTLCIISFATVLSILIIYMDTTEIRHQQFRLNMSRDYDLMGVPQDNPALVKYVRDIHMKKYPMTFMRNANAPVEQLNFTNRHELTPEMAHLISDLLGRKRFGTFFQSLTGNSDSMMTAPWLAETMNWGGYLVEPDPRKYFSLRKQNAFRSNVQVIHACLSPNTYPKEVTLHHDDDSEVQINSVLDEETEWFHPRVKCFPVYTLMLAVNRTSIDLLSLGCHGQELQILQTIPFDRVHIKVISIHLVHYFEEDEMMIDVDEYVQSIGRFLMSKSYKLVRSMDRNFIYQHLSVLSAAKQRQRQQQQQQSFSVADHMLASSQQHQQQQQHPVPARSNAISSGEKPDATMAIRKGQEISSNSGKIPMSLSSAATTTPVPATTTSTTAVPSSTAARATDSSERFDKLMLLRGGDSEPFDASDSEFAGSSDSSAMESGSDSEDEAQQQQAEQDKPAEVVDLEQTVSLMEPLDETELEDGSVRVAGSSSPPPMVTSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W862</accession>
    <name>A0A6E8W862_ANOCL</name>
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        <submittedName>
            <fullName evidence="7">STAS domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
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        <lineage>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
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        <subcellularLocation>
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            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_040363500.2"/>
    </dbReference>
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    <dbReference type="EnsemblMetazoa" id="ACON013218-RA">
        <property type="protein sequence ID" value="ACON013218-PA"/>
        <property type="gene ID" value="ACON013218"/>
    </dbReference>
    <dbReference type="GeneID" id="120947789"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_014552"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON013218"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035249"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        </location>
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            <end position="236"/>
        </location>
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        </location>
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        </location>
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        </location>
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            <begin position="492"/>
            <end position="569"/>
        </location>
    </feature>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblMetazoa" id="ACON013218-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="599" mass="65043" checksum="B05AEC2628E8F77C" modified="2023-06-28" version="2">MEKPGEKPLLKSVFTGENGDVEEDCSEKFPSVGPMLTSKLGGFCNRKFIFKRLPILQWLPAYEARFLLEDIVAGLSVGLTVIPQGIAFAVMANLEPQYGLYSAFMGCFVYCVFGSCKDLTIGPTAIMALMVQVYVGSLGANFAILLTFLTGCIILMLGLLNLGFLVQFISMPVTAGFTSAAAITIASGQVKSLLGLPGRSNEFVDSWINVYEHIGQTKLWDAVLGFTTIGVLLLLRSLRSKWSLMGKYLALSRNAVVVIGGAALAYYCSTFGSKPFSLTGTVTPGLPPIKLPPFTTVLNNETLAFNEMTSMLGSSIIALPLIAILETIAIVKAFSKGKSIDASQELVALGMCNIFGSLVSSMPITGSFTRTAVNNNSGVRTPLGGIVTGVLVLLSLGLLTDTFYFIPKTVLAGVMIAAMFFMIEFHAALEIWRTKKVDIIPFMVTLVSCLLLGLEYGMLIGIALNICFVLYMTSRPNIDQALLRTSSGIEAMVVKPDQSLIYSSIEYLKHEVVKRTDKTQVTTVIIDGSNVSYVDSTAAKIFSSTIEELALRGRTVLLWNWNRSVRCTLIRLNKVQFHPLFKNGGLEQLDKEICNESLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W9Y0</accession>
    <name>A0A6E8W9Y0_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="3">PH domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8W9Y0"/>
    <dbReference type="EnsemblMetazoa" id="ACON013121-RA">
        <property type="protein sequence ID" value="ACON013121-PA"/>
        <property type="gene ID" value="ACON013121"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_013005"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON013121"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_043323"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045595">
        <property type="term" value="P:regulation of cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039680">
        <property type="entry name" value="PLEKHB1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14309">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14309:SF12">
        <property type="entry name" value="PH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON013121-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="242" mass="26809" checksum="327522BFCE5EA8B3" modified="2020-08-12" version="1">MFSLCAKPASGNGSSRTSQQLQQVTCKSCNSTVGKQIKGGYLLRYKKQVLWSRWSEEWVVLYDDSTMAWFTDWIAAITKTLPPPPQIELVVDKPHLMGVLRRPIVRIRPATSSEVSARRKNRHTIEHNTTKVKGEAAVAIIKKNAQDTTDPKGSLACALPWGHGWGWATLPNGIWSGALTWSQCEDSITLHALPTTHCTNMIETPCAGGIYHTDIGPFDFHGIEDVGGEDFDYAMDCGDFIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W9Y3</accession>
    <name>A0A6E8W9Y3_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DUF4766 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8W9Y3"/>
    <dbReference type="EnsemblMetazoa" id="ACON013397-RB">
        <property type="protein sequence ID" value="ACON013397-PB"/>
        <property type="gene ID" value="ACON013397"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001909"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON013397"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_036773"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="114"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="239"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="114"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="125"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="239"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="281"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON013397-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="338" mass="33880" checksum="80A2F7AF03926313" modified="2023-06-28" version="2">MRVATERNRMKCSCAVVKVPLVELASGFIILLIAVLLPMAAYAKPASPSLDPTDLAEVELLTEDVARIKRSGFGSGSGSFDLLSGLKKTLLSGIGSASASLVAGSSSSSLSSGLGHGSSGGSAGHGSSYGSSSGHSYDKPHEDTHFDGWSLKKSILNTLFQAVKAITGGVTAIKGQLIKGSGYLVSGAGKLIASGGDAVTGVGKKIALSAHLIPPKHSAHPLSKLTGILSASSSGLSGLSGSSSSSSSGHGHGHGDASGESFTSYEGPTSYGHDSSHGALSGPSGPSYIPPSKGSHKAPSHNSYGADLDDHHPHSSYVPAGKYGHSSDDSDYSKIVVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9I3A7E1</accession>
    <name>A0A9I3A7E1_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Serine/arginine repetitive matrix protein 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2023-03" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A9I3A7E1"/>
    <dbReference type="EnsemblMetazoa" id="ACON000294-RB">
        <property type="protein sequence ID" value="ACON000294-PB"/>
        <property type="gene ID" value="ACON000294"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041869"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032064">
        <property type="entry name" value="DUF4805"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16063">
        <property type="entry name" value="DUF4805"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="283"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="381"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="17"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="403"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="452"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000294-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="555" mass="60895" checksum="60283B60AEAAF97A" modified="2023-06-28" version="1">MMSSYRHSTANGDGACDVNNNNNSNDDHNNNLLTADNLLRLALKKPKSWNWELTTSSSSPNITFPKIQLFDGASGELMVEVDRPDRVIRSGCPDTVPKSGGSSPSRYRRSRTSLVREKLTTTGDSLADIFSQLQNKGLGHKLTTSGSQYRLLQGAQVDESARGSLQKSKSANAIVVTPEAPPIVRRRSRRSLASRSSSILERISEFYGRSSTEEEDVLPAVPQLLLAPASEAGSPAQDEGVTIEELPADDCPAETAINGPPRPKIYKLVRSNIGTLMVREESFHTQRSLRRRQREAGGERVPSPPELVELDEQTDRPRPAAGGIELDRNRYEREISRIDGLLSRVMLSHDLQTEELARADLGPSIRIEDAPEDALVREVMNGLSTEHQNGSTTRRPRRRSRRSVSVGGSVTSPHRLRSASSSSTSDGEQDRGYRGTRLRGQHRTGSSSRSGSQKRRGRTRHRDPDVGPHATISNGSHSWRKKPCGASARVQLIKPARSGLSHRSDLLCVRSSSSAPCQCVLFVLTQRQCLCCVSVCVCGCALLIRSVVASLMKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9I3B275</accession>
    <name>A0A9I3B275_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Chitin-binding type-2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2023-03" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_040231263.2">
        <property type="nucleotide sequence ID" value="XM_040375329.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9I3B275"/>
    <dbReference type="EnsemblMetazoa" id="ACON010783-RB">
        <property type="protein sequence ID" value="ACON010783-PB"/>
        <property type="gene ID" value="ACON010783"/>
    </dbReference>
    <dbReference type="GeneID" id="120954955"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035893"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.140.10">
        <property type="entry name" value="Chitin binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002557">
        <property type="entry name" value="Chitin-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036508">
        <property type="entry name" value="Chitin-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051940">
        <property type="entry name" value="Chitin_bind-dev_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23301">
        <property type="entry name" value="CHITIN BINDING PERITROPHIN-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23301:SF106">
        <property type="entry name" value="CHITIN-BINDING TYPE-2 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01607">
        <property type="entry name" value="CBM_14"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00494">
        <property type="entry name" value="ChtBD2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57625">
        <property type="entry name" value="Invertebrate chitin-binding proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50940">
        <property type="entry name" value="CHIT_BIND_II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0147">Chitin-binding</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039907521" description="Chitin-binding type-2 domain-containing protein" evidence="6">
        <location>
            <begin position="23"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="7">
        <location>
            <begin position="23"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="7">
        <location>
            <begin position="80"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010783-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="131" mass="14445" checksum="FEE5C19E35009A00" modified="2023-06-28" version="1">MQGSVSTVWIALLALGVSAITAEPTCRPTGQYLTANPRDCRSYFYCYDGIAYYGVCQQGFRFDESRQSCLPSTVAECFECPTMGMVSLPHPTSCQKFVLCFEGVANERSCPTGLLFNRQVHQCDLSAKVIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABM2BEX3</accession>
    <name>A0ABM2BEX3_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DUF4704 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_040227141.2">
        <property type="nucleotide sequence ID" value="XM_040371207.2"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON030777.R9">
        <property type="protein sequence ID" value="ACON030777.P9"/>
        <property type="gene ID" value="ACON030777"/>
    </dbReference>
    <dbReference type="GeneID" id="120952088"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd06071">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01201">
        <property type="entry name" value="PH_BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1540.10">
        <property type="entry name" value="BEACH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000409">
        <property type="entry name" value="BEACH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036372">
        <property type="entry name" value="BEACH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050865">
        <property type="entry name" value="BEACH_Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046851">
        <property type="entry name" value="NBCH_WD40"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031570">
        <property type="entry name" value="NBEA/BDCP_DUF4704"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023362">
        <property type="entry name" value="PH-BEACH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13743:SF112">
        <property type="entry name" value="BEACH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13743">
        <property type="entry name" value="BEIGE/BEACH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02138">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15787">
        <property type="entry name" value="DUF4704"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16057">
        <property type="entry name" value="DUF4800"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13385">
        <property type="entry name" value="Laminin_G_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20426">
        <property type="entry name" value="NBCH_WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14844">
        <property type="entry name" value="PH_BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01026">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81837">
        <property type="entry name" value="BEACH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50197">
        <property type="entry name" value="BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51783">
        <property type="entry name" value="PH_BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="BEACH-type PH" evidence="7">
        <location>
            <begin position="1952"/>
            <end position="2049"/>
        </location>
    </feature>
    <feature type="domain" description="BEACH" evidence="6">
        <location>
            <begin position="2062"/>
            <end position="2354"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="2504"/>
            <end position="2545"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="2587"/>
            <end position="2628"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="726"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1439"/>
            <end position="1460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1475"/>
            <end position="1497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1891"/>
            <end position="1912"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="1820"/>
            <end position="1847"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1443"/>
            <end position="1457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1901"/>
            <end position="1910"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON030777.P9"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2762" mass="310488" checksum="375FA38A48EDC81D" modified="2025-10-08" version="1">MDSRKDIYNLWVQYTTKNEETYFRQFIAGFVSIWHSQLQLDFDNAPRWDAVAPDLGPHLSRLPDELLPAIEKFLIIARDGCENGRNEEGEETGVHGSSMRENVLLVQCLTVICRHFENIAAVINSSYISSCIAISNFIITRLNDERGEPKEQEADLLFVASVARLLEVLYDPYLTWRSYLRGHHADYASLGYKISQLQPEIVPFIYDCFQGGKVTKHARIGKDLVHILGAVIAGSQHNGLRAISPATVSIIIDVVCKWESNAELRQLMLHCFALMAVILQKSSPEQRQIDLLTIFQLYIGSIRDLLTTEHFLPKSSSVGSFELSHDDDNYIDVSALCAAVAIIRDFIPDHANKLILCNALFEAKFLANLVQIPEQVKKWNIDHQTLGTVLVETLQKLCYHSEKMQHNLMHTNSINVLFDGLREFGKPSASLISQCLGFAFDEHDTRQANPVVVTKLIEWIPLMGEQEQNMMSEVLLRKCAGSMQSKQLVCSQRTIKRIVDVCLSRCQRLSSKCAMNLLKLIEELAKYSILPAEVNSLFRLLRPDAQFEYRKLLLETILRITQHRSSAGSMPDNFLDIQTNTSGITIPEIRKWETTHGFVFHAWLRLEEERSIAQNYRRQLFSLTTNYGTGYEFFVQKNGNFVVSVITKKEFFTATVTSPQLLDGRWHSVTVSVVPPKRLFSYHQINVYLDSVQKLGSTMKYASFAEVFQYCSIGAPYNNFRKVSHTSHQSNSARMSPVPDPGVASPSSEREEKTKPSGLFPNLIERTFFPGLVSQVPNYFTLPSKSTSSLDPSVKSYPIGMQDLVFGEAICLKGQLGAVLLADTNINLKSLFEAGPSVANVLATDMIESFDMASKFVFCFAPNACFDGLCLDLAPGNQYNGHLVASVAHTLSLQNALNGIGGMVALLPIVDSLALLPDADLPSANELISLSPVESPLKEERFGDWEMLQLNSLTEAKIIQNPIACFFCLVRNFISGSELNKENMLKNDAIAIISTLLQRCNERLFDVNVLMAVQLLIESIQNEMPSGNMELLHVLHRDLVFNFRIWAKAQFQIVIGHVQYISTIIKDDRKYFRRHFGIQYLLDVIQEHFTNNASLPPQDAKMVRESLLRIVKYYIQKEVNIKEISAILTFLTTTKHEATVGEIIDMLVLLIEGKHVKDQIFLLMYEPHMAEAMYMLFIDRHFGSEVHMKLIKFITGMLCTKRISAKHKAALRLYDTTIECHSLYPGLFSFMLPFQLEPHVILGLLDLNLSSDTEMGYAGALCLMYHSNLLELSLKLEIARRLMTSTFTKPKAPQMIAKQVGWQESIARLLIKKPVENRSEEGAEKGKQNLLTDMDEVVKRESESSAAGPGADDLIVFDEENLELHVQESGKQPNSFMSEAATVLEHEIKELADTVQEAVVDNIASSITSVYSVIRQKTSDIQDTIGSLTLSFEESAGRKKKSSLSSSSEENVSLDESGSLAGGTASMVVEDLQSTKSGEELDSSAGNSVDSASEKYADDNEEENLVCSVSSSLFTILWRGVENSNDSWQERGQVMACINLIALNNELYCSHLALRLRILEMGVQAALMDLAENAHLALIHQQNAAQLLRLAYDLVVLDPNEDDSKKCSVKLLDGVLSLLDILMVFQQSSSDDWSEMSHLCLGLLLKCSHSANSEIVAMATAKLHALLQNRTNQDPAEIGYLMYSLNQAMCNAIEVDNAEQYSFLIPVMKALLEKSRTVLGLATHTPDLPATSSGPIFFQDFQTYCASRQWTTFIDKKIKPLYTSYQKDLSITLWEPLNTFWAECYESCKLSSQRRAKLQMESRRKFQERILVPCRIRQSEENARLSIQQAQRKAKDANTERRWLNLQRFLYGPKGAWAKETREEEYWRLSHHENVARMRLKLEPDLYHDPHTAAANMRDNTTSSTSQSRRLSSEFGSTFAPAAIGEENTDDEMLLLEEEMRNSLEPQVPETSGREKIVITQECELITLMTRVKGRFDLTTNQLMFTEIASLKDDGQRHDFKFPIGQLRELHLRKFNLRRSALEMFLTDQTSYFLNFTTRTRNKIFTKILSLQPPNILYGSGRSPAELLKSSGLTQKWANREISNFEYLMHLNTIAGRSYNDLSQYPVFPWILADYTSEVLDLNDPRSFRDLSKPIGVVNPKNEAEVRSKFDGFEDPSGMIPKFHYGTHYSNSAGVLHYLIRVEPFTSLHIELQSGRFDVADRQFHSIPQTWKLLMDNPNDVKELIPEFFYFPEFLKNMNRFDLGVLQMTKEKVDDVVLPPWAKTAEDFIAIHRRALESEYVSQHLHHWIDLIFGYKQKGPKAVDALNVFYYCSYEGAVDLDKIANPIEREAVEGMINNFGQTPSQLLRDPHPRRLSLDELTIRLLKLELRRPDLTLILDRVQCTACDLSTDKDPVVYLSTPKSPPRSFLQTSPDMLITVTKSGILGCHSWMSFDKERGFLLEIDATTLNLKNRKKLTGPFHPSITLSSKLFALSVDAKYIYAGGIWDNSVRVFNMARGKVVASAINHFDVVTCIAMDNCGSYLVTGSKDCTCVIWSLSTSNSVSQPAGSNLQPNTAALNQNLAGNVVGSANVVHLTNNLTPKPVHTLYGHDDAVSCVAIMTELDMVVSGSLDGTVNVHTIKNGQFIRTINPIGCTGCKIEVSFITISYQGHIAFSALDDTSHSVHVFSINGVSLGSKYVSGRVTGLTSSSDYLVVSDDAGDVTMSRLYGLKPIFDIPLHVPTQTVVGTVGNTHLLAPLRDGSLGVIGIQQPIPYKKHTVLNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A182KXP0</accession>
    <name>A0A182KXP0_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">long-chain-fatty-acid--CoA ligase</fullName>
            <ecNumber evidence="7">6.2.1.3</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a long-chain fatty acid + ATP + CoA = a long-chain fatty acyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15421"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57560"/>
            <dbReference type="ChEBI" id="CHEBI:83139"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:15422"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.3" evidence="7"/>
    <dbReference type="RefSeq" id="XP_049462371.1">
        <property type="nucleotide sequence ID" value="XM_049606414.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_049462372.1">
        <property type="nucleotide sequence ID" value="XM_049606415.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_049462373.1">
        <property type="nucleotide sequence ID" value="XM_049606416.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A182KXP0"/>
    <dbReference type="STRING" id="1518534.A0A182KXP0"/>
    <dbReference type="EnsemblMetazoa" id="ACOM028692-RA">
        <property type="protein sequence ID" value="ACOM028692-PA.1"/>
        <property type="gene ID" value="ACOM028692"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON003623-RC">
        <property type="protein sequence ID" value="ACON003623-PC"/>
        <property type="gene ID" value="ACON003623"/>
    </dbReference>
    <dbReference type="GeneID" id="120953788"/>
    <dbReference type="CTD" id="46068"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_008993"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACOM028692"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003623"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035053"/>
    <dbReference type="Proteomes" id="UP000075882">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090433">
        <property type="term" value="F:palmitoyl-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035336">
        <property type="term" value="P:long-chain fatty-acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000026">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272:SF83">
        <property type="entry name" value="ACYL-COA SYNTHETASE LONG-CHAIN, ISOFORM J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="9">
        <location>
            <begin position="117"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="10">
        <location>
            <begin position="583"/>
            <end position="624"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00026121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003623-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="707" mass="79062" checksum="8D0D24D287B395CA" modified="2016-09-07" version="1">MESVWVQSAIGAIKIITMVYDIITMPIYLAIQRPWKRRQLARRVKAKIIQQDSSSITYRSVDSPGEMHVKMMQNNIDTLERMFNFVTKVHTTKRCIGTRQILGEEDEMQPNGRMFKKFRMGDYVWRNFIETEHAAACFGRGLRELGQEPKQNIVIFAETRAEWMIAAHGCFKQNMPVVTIYATLGDDGVAHGINETEVTTVITSHELLPKFKSVLNVTPNVKKIIFMEDQLHPTDTTGFKEGVEIIPFSKVIEIGNTSTIPGSPPAAEDTAIIMYTSGSTGTPKGVLLSHANCIGTMKNFCDIFKIYPDDVLIGFLPLAHVFELLAESVCLLTGVPIGYSTPLTLIDSSSKVMKGCKGDASVLRPTCMTSVPLILDRISKGINDKVNAEAPMKKAFFKFAYNYKSKWTARGYQTPLMDKILFKKIAKLLGGRIRSVLSGGAPLAPDTHEQIKLCLCVDVIQGYGLTETTAGAAVMDKWDMEYSRVGAPSSSNDIRLINWEEGNYRVTNKPYPQGEIVVGGTTVSKGYYKLPGKTQEDFFEEDGQRWFRTGDVGEIHPDGALKIIDRKKDLVKLQAGEYVSLGKVESELKTCPVVENICVYGDSTKQYTVALVVPNPKHLEEIAERLDIRGVEFEDLCDNKKLEKAVLQELADHGRKCKLHRSEIPAAVHLCKDIWTPDMGLVTAAFKLKRKDIQERYQAEISKMYAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A182LLQ5</accession>
    <name>A0A182LLQ5_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Methenyltetrahydrofolate cyclohydrolase</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(6R)-5,10-methenyltetrahydrofolate + H2O = (6R)-10-formyltetrahydrofolate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57455"/>
            <dbReference type="ChEBI" id="CHEBI:195366"/>
            <dbReference type="EC" id="3.5.4.9"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NAD(+) = (6R)-5,10-methenyltetrahydrofolate + NADH</text>
            <dbReference type="Rhea" id="RHEA:22892"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:57455"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.5.1.15"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040228695.1">
        <property type="nucleotide sequence ID" value="XM_040372761.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040228696.1">
        <property type="nucleotide sequence ID" value="XM_040372762.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_049462489.1">
        <property type="nucleotide sequence ID" value="XM_049606532.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A182LLQ5"/>
    <dbReference type="STRING" id="1518534.A0A182LLQ5"/>
    <dbReference type="EnsemblMetazoa" id="ACOM040079-RA">
        <property type="protein sequence ID" value="ACOM040079-PA"/>
        <property type="gene ID" value="ACOM040079"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON004677-RA">
        <property type="protein sequence ID" value="ACON004677-PA"/>
        <property type="gene ID" value="ACON004677"/>
    </dbReference>
    <dbReference type="GeneID" id="120953082"/>
    <dbReference type="CTD" id="47895"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012517"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACOM040079"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004677"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030130"/>
    <dbReference type="Proteomes" id="UP000075882">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004477">
        <property type="term" value="F:methenyltetrahydrofolate cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004487">
        <property type="term" value="F:methylenetetrahydrofolate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004488">
        <property type="term" value="F:methylenetetrahydrofolate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01080">
        <property type="entry name" value="NAD_bind_m-THF_DH_Cyclohyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10860:FF:000001">
        <property type="entry name" value="Bifunctional protein FolD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000070">
        <property type="entry name" value="probable bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01576">
        <property type="entry name" value="THF_DHG_CYH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000672">
        <property type="entry name" value="THF_DH/CycHdrlase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020630">
        <property type="entry name" value="THF_DH/CycHdrlase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020867">
        <property type="entry name" value="THF_DH/CycHdrlase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020631">
        <property type="entry name" value="THF_DH/CycHdrlase_NAD-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48099:SF11">
        <property type="entry name" value="BIFUNCTIONAL METHYLENETETRAHYDROFOLATE DEHYDROGENASE_CYCLOHYDROLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48099">
        <property type="entry name" value="C-1-TETRAHYDROFOLATE SYNTHASE, CYTOPLASMIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00763">
        <property type="entry name" value="THF_DHG_CYH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02882">
        <property type="entry name" value="THF_DHG_CYH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00085">
        <property type="entry name" value="THFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00766">
        <property type="entry name" value="THF_DHG_CYH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00767">
        <property type="entry name" value="THF_DHG_CYH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tetrahydrofolate dehydrogenase/cyclohydrolase catalytic" evidence="13">
        <location>
            <begin position="5"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="Tetrahydrofolate dehydrogenase/cyclohydrolase NAD(P)-binding" evidence="14">
        <location>
            <begin position="139"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00036357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00050302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00061364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004677-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="303" mass="33057" checksum="E24FC8D408DCE640" modified="2016-09-07" version="1">MAQLIDGKQIAREIQEELRKKIETWKAQGHRSPQLTAILIGDDPASTTYVNNKMKAAADVGIESKTERFGTEITEEQLLERIDQLNNDDAVDGILIQLPVPNHIDERKVCNAVSCDKDVDGFNERNIGRLCLDMNTLIPCTPLGVQELIKRTGIETFGKNAVVVGRSKNVGMPIAMLLHADGRNDTCAMDCTVTICHRFTPPQELKRFCRTADIIVTATGVPGLITSDMIKEGAAIIDVGITRITDPSTGKKKLVGDVDFEEVRKKAGLITPVPGGVGPMTVAMLMKNTIIAAKNLAKKRNES</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8UZ49</accession>
    <name>A0A6E8UZ49_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Suppressor of cytokine signaling 7</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
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    <comment type="pathway">
        <text evidence="5">Protein modification.</text>
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        <property type="gene ID" value="ACON000880"/>
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    <dbReference type="VEuPathDB" id="VectorBase:ACON000880"/>
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    </dbReference>
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        <property type="term" value="C:phosphatidylinositol 3-kinase complex"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Suppressor of cytokine signaling 2"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="SUPPRESSOR OF CYTOKINE SIGNALING 7"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00253">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="1" id="KW-0341">Growth regulation</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="32"/>
            <end position="138"/>
        </location>
    </feature>
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        <location>
            <begin position="133"/>
            <end position="178"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00022604"/>
        </source>
    </evidence>
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        <source>
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        <source>
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    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000880-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="204" mass="23816" checksum="3ABBC04BD4959E5F" modified="2020-08-12" version="1">MGDTSDQKKKSNERKSSMDFTASIEKVQEFGWYWGPITSEGAEKILSNEPDGSFLVRDSSDDHYIFSLTFKLNGTVRHVRIDQYQGSFSFGSCAKFKSRTIMEFIENAVEHSRSGRYLFFLHRRPEYGPMRVQLTKPVSRFKHVQSLQHICRFVIHKTIKRKDLIQALPLPRRVLDYLCYKNCLSERTEVDLLSSLEKPNIVLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
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    <name>A0A6E8UZA0_ANOCL</name>
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    <organism evidence="5 6">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8UZA0"/>
    <dbReference type="EnsemblMetazoa" id="ACON000969-RA">
        <property type="protein sequence ID" value="ACON000969-PA"/>
        <property type="gene ID" value="ACON000969"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006835"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000969"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_036786"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030016">
        <property type="term" value="C:myofibril"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005523">
        <property type="term" value="F:tropomyosin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030239">
        <property type="term" value="P:myofibril assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051694">
        <property type="term" value="P:pointed-end actin filament capping"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Tropomodulin, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="entry name" value="TMOD"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10901:SF6">
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="2"/>
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    </dbReference>
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    <feature type="region of interest" description="Disordered" evidence="4">
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            <end position="71"/>
        </location>
    </feature>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000969-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="366" mass="40825" checksum="0DF4F700900D18B0" modified="2020-08-12" version="1">MTSSSTTTKTITTPAKLYGKDVGAYDDIDVDELLAQLSPEEITMLAKEVDPDDSFLPPSQRTNYECDKAPTGPLDRKKLIDHINKQALETPDRPELEPFVPGVVRGKKWIPPPQAAKLQAADEQIAIDLGDEYEQALSDATQEEIIDLAAILGFHSMMNQDQYHASLLNKGQPVGLGWDGITKSAIPKIFPAEAPNTTDPEETIQRIKSDDSKLVEVNLNNIKTISDEQFDKLFEALKSNTHLEVLSLTNVGLTDRTALMLAAALEQNHALRVLNVETNFISPTVIVTLVKSLLTQMTIEEFRASNQRSQVFGNKIEMEITELVEKNTTLLRLGLHLEFNDARHRVAAHLQRNIDRIRQARLTQSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V2C3</accession>
    <name>A0A6E8V2C3_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Phosphatidylglycerophosphatase and protein-tyrosine phosphatase 1</fullName>
            <ecNumber evidence="11">3.1.3.27</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phospho-(1'-sn-glycerol-3'-phosphate) + H2O = 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phospho-(1'-sn-glycerol) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:42304"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:75163"/>
            <dbReference type="ChEBI" id="CHEBI:78907"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:42305"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>1,2-dibutyryl-sn-glycero-3-phospho-(1D-myo-inositol-5-phosphate) + H2O = 1,2-dibutyryl-sn-glycero-3-phospho-(1D-myo-inositol) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:42584"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:82605"/>
            <dbReference type="ChEBI" id="CHEBI:82606"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:42585"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>1,2-dioctanoyl-sn-glycero-3-phospho-(1D-myo-inositol-5-phosphate) + H2O = 1,2-dioctanoyl-sn-glycero-3-phospho-(1D-myo-inositol) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:42308"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:65221"/>
            <dbReference type="ChEBI" id="CHEBI:78911"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:42309"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycero-3'-phosphate) + H2O = a 1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycerol) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:33751"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:60110"/>
            <dbReference type="ChEBI" id="CHEBI:64716"/>
            <dbReference type="EC" id="3.1.3.27"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:33752"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a 1-acyl-2-hexanoyl-sn-glycero-3-phospho-(1D-myo-inositol-5-phosphate) + H2O = a 1-acyl-2-hexanoyl-sn-glycero-3-phospho-(1D-myo-inositol) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:42320"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:78930"/>
            <dbReference type="ChEBI" id="CHEBI:78931"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:42321"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism.</text>
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    <comment type="pathway">
        <text evidence="10">Phospholipid metabolism; phosphatidylglycerol biosynthesis; phosphatidylglycerol from CDP-diacylglycerol: step 2/2.</text>
    </comment>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
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        <property type="gene ID" value="ACON000617"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="entry name" value="PHOSPHATIDYLGLYCEROPHOSPHATASE AND PROTEIN-TYROSINE PHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
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    </dbReference>
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        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
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    </dbReference>
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    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="9" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="5" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="18">
        <location>
            <begin position="28"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="19">
        <location>
            <begin position="106"/>
            <end position="184"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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            <dbReference type="ARBA" id="ARBA00069309"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000617-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="201" mass="23299" checksum="CA723F5932C021C5" modified="2020-08-12" version="1">MTAMFARITFYPSLFYNVMMEKITARHWYDRIDENVILGALPFRSVAQEMVQKENIKAVVSMNEDYELWAFSNNKARWAKLGVEFLQLETTDIFESPCQEKLWKGVNFMNRFLPANDRMALLPAAPGELAEPGTIYVHCKAGRTRSATLVGCYLIMKNGWSPEQAVEHMRQCRPHILLRSKQWEALRLFHQTKCPPPKTPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V312</accession>
    <name>A0A6E8V312_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Tropomodulin</fullName>
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    </protein>
    <organism evidence="5 6">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
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            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
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            <taxon>Anopheles</taxon>
        </lineage>
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    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
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        </subcellularLocation>
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    <dbReference type="Proteomes" id="UP001105220">
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        <property type="project" value="InterPro"/>
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    </dbReference>
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        <property type="entry name" value="Tropomodulin, isoform C"/>
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        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="LRR_dom_sf"/>
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        <property type="entry name" value="TMOD"/>
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        <property type="entry name" value="TROPOMODULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </evidence>
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    </evidence>
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000969-PE"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="415" mass="46774" checksum="329DB19CBF4467C9" modified="2020-08-12" version="1">MAETYEEYEETHHVTRKSVTTFKIEETSSSTTTKTITTPAKLYGKDVGAYDDIDVDELLAQLSPEEITMLAKEVDPDDSFLPPSQRTNYECDKAPTGPLDRKKLIDHINKQALETPDRPELEPFVPGVVRGKKWIPPPQAAKLQAADEQIAIDLGDEYEQALSDATQEEIIDLAAILGFHSMMNQDQYHASLLNKGQPVGLGWDGITKSAIPKIFPAEAPNTTDPEETIQRIKSDDSKLVEVNLNNIKTISDEQFDKLFEALKSNTHLEVLSLTNVGLTDRTALMLAAALEQNHALRVLNVETNFISPTVIVTLVKSLLTQMTIEEFRASNQRSQVFGNKIEMEITELVEKNTTLLRLGLHLEFNDARHRVAAHLQRNIDRNDPEHCHPIPYYRFLHVPIPTEPVPTKRTWEELP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V4E1</accession>
    <name>A0A6E8V4E1_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glyco_18 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8V4E1"/>
    <dbReference type="EnsemblMetazoa" id="ACON001863-RA">
        <property type="protein sequence ID" value="ACON001863-PA"/>
        <property type="gene ID" value="ACON001863"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006431"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001863"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040932"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004568">
        <property type="term" value="F:chitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011583">
        <property type="entry name" value="Chitinase_II/V-like_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029070">
        <property type="entry name" value="Chitinase_insertion_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050314">
        <property type="entry name" value="Glycosyl_Hydrlase_18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11177">
        <property type="entry name" value="CHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11177:SF144">
        <property type="entry name" value="CHITINASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00636">
        <property type="entry name" value="Glyco_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54556">
        <property type="entry name" value="Chitinase insertion domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026263858" evidence="2">
        <location>
            <begin position="23"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="GH18" evidence="3">
        <location>
            <begin position="23"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001863-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="377" mass="42656" checksum="FB01145AA3F68308" modified="2020-08-12" version="1">MCWAFTGFILTVLAASKVFVEAERVVCSFTSGAVYRRGEYAFQMTDIPVSLCTHVVYDRLAIDLYSKDTPYFTLTNPDADEPFDEFEKFSALKRTNPQLKLIICLRSIFIAQVASEPDQRRALIERLVQFMSTYNLDGVELFWADERFKSEESLYLLLEEVKSGFRAANYPTWEVTILIDIDHRGLDHARLCRLLDYVHIAGAGERKPKYGNNSTMPSANALFDVDDQTNMTLNDALQYWIDKGCPADKIVLGVVFIAQVFQLKKPNTVKTPMTYCTVPEDGPFCAYIEMCQKFNESDWTMGWDDTAGLAPHAFQTDYWAAYENEASVGRKGEIAREKGLAGVYAVALDLDDYRGKCGPAYPLLKSLCGSYRQKANN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V8L6</accession>
    <name>A0A6E8V8L6_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">hydroxyacylglutathione hydrolase</fullName>
            <ecNumber evidence="5">3.1.2.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Glyoxalase II</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>an S-(2-hydroxyacyl)glutathione + H2O = a 2-hydroxy carboxylate + glutathione + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21864"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58896"/>
            <dbReference type="ChEBI" id="CHEBI:71261"/>
            <dbReference type="EC" id="3.1.2.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.6" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8V8L6"/>
    <dbReference type="EnsemblMetazoa" id="ACON000416-RA">
        <property type="protein sequence ID" value="ACON000416-PA"/>
        <property type="gene ID" value="ACON000416"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON000416-RB">
        <property type="protein sequence ID" value="ACON000416-PB"/>
        <property type="gene ID" value="ACON000416"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_008266"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000416"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030396"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004416">
        <property type="term" value="F:hydroxyacylglutathione hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019243">
        <property type="term" value="P:methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031123">
        <property type="term" value="P:RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07723">
        <property type="entry name" value="hydroxyacylglutathione_hydrolase_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.15.10:FF:000019">
        <property type="entry name" value="Hydroxyacylglutathione hydrolase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01374">
        <property type="entry name" value="Glyoxalase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035680">
        <property type="entry name" value="Clx_II_MBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032282">
        <property type="entry name" value="HAGH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017782">
        <property type="entry name" value="Hydroxyacylglutathione_Hdrlase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03413">
        <property type="entry name" value="GSH_gloB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11935">
        <property type="entry name" value="BETA LACTAMASE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11935:SF94">
        <property type="entry name" value="TENZING NORGAY, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16123">
        <property type="entry name" value="HAGH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005457">
        <property type="entry name" value="Glx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="10">
        <location>
            <begin position="14"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000416-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="265" mass="29404" checksum="B4A8EA793A8DF373" modified="2020-08-12" version="1">MASMTVTKIPALKDNFMYLVVCNATRQAAVVDPVEPARVLEVAREQGCKLNQLLTTHHHWDHAGGNEALCEQYRQHADWGQLTVYGGDDERIPGLTNRVGQDDTFAIGQLRVRCLATPCHTTSHVCYYVEGGDRGERAVFTGDTLFLAGCGRFFEGTPDQMYDALIGKLSALPDDTRVYCGHEYALQNLRFGHQVEPDNADTRALLERAQAADLEGRRALVPSTIGQEKRINVFMRVHQPAVQAYVGKGTPLETMQALRAAKDKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V8P6</accession>
    <name>A0A6E8V8P6_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_040224068.2">
        <property type="nucleotide sequence ID" value="XM_040368134.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8V8P6"/>
    <dbReference type="EnsemblMetazoa" id="ACON000483-RC">
        <property type="protein sequence ID" value="ACON000483-PC"/>
        <property type="gene ID" value="ACON000483"/>
    </dbReference>
    <dbReference type="GeneID" id="120950241"/>
    <dbReference type="CTD" id="6670"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006804"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000483"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040123"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045334">
        <property type="term" value="C:clathrin-coated endocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043812">
        <property type="term" value="F:phosphatidylinositol-4-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001135">
        <property type="term" value="P:regulation of endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034753">
        <property type="entry name" value="hSac2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022158">
        <property type="entry name" value="Inositol_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002013">
        <property type="entry name" value="SAC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45662">
        <property type="entry name" value="PHOSPHATIDYLINOSITIDE PHOSPHATASE SAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45662:SF8">
        <property type="entry name" value="PHOSPHATIDYLINOSITIDE PHOSPHATASE SAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12456">
        <property type="entry name" value="hSac2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02383">
        <property type="entry name" value="Syja_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51791">
        <property type="entry name" value="HSAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50275">
        <property type="entry name" value="SAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAC" evidence="2">
        <location>
            <begin position="197"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="domain" description="HSac2" evidence="3">
        <location>
            <begin position="589"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="807"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="984"/>
            <end position="1034"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="813"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="828"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="857"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="992"/>
            <end position="1005"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1020"/>
            <end position="1030"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000483-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1101" mass="118524" checksum="B884384F3DAFCEB4" modified="2020-08-12" version="1">MAMEVFQTETHYIFVRKERSLWWNRYSSEFQLKCGWDLSSVDDIECIGVTHGIVGTIALQGVLDPHLIVIKEVVPVGVLYAPHLVYKIRSIAVLGPDGADTVLLGCSKHTTSQTVKVAPAGGTAAPEGHPSPAKGRLFESSALVNKTWGAVKSAGSTIRNTTEKAAAIATSQVKSTVSLVVKADPVRIEKRVLDELHKIFDETDSFYYSPNCDITNNLQRRGAPPDDRFYWNRPMLAELLRVVSEQPGGEDWVVPIIQGFVQVEQCVIGNECFTLALVSRRSRNRAGTRYKRRGVDEHGNCANYVETEQVLSLRQHQISFTQVRGSVPVYWSQPGYKYRPPPRLDRDEPETQAAFERHFDGELQIYQSICIINLVEQSGKEKIIGDAYANHVLKYNSDRLIYVTFDFHEYCRGMRFENVSSLIESLAPEAGAMGFHWRDTNGPICNQKGVFRVNCMDCLDRTNVVQTAIGKAVLESQLVKLGLAMPYSQLPDALKAPFMVLWANNGDVISRQYAGTNALKGDYTRTGERKISGIMKDGMNSANRYYLARFKDTYRQATIDMMLGNNVSPESVSALGGTQTAATDETDALEGAEHARLLVEDCRRLLLGTAQLPVGAWGLIDADPSTGDPSETEVDTILILTDDCYIVAEYDSHLDKIVRFENVPLASITLIELGLFQHSKMFQGPAAAHLCVRLNYAVDGVDGYFHMFRSPNIRFFNNVAVVIRKPEEIAESLTAIVDLFRIALQNCGRTEVAFQCGGSLQRRKSRTLLSVPAGIPRNLSESQLVQMGSKAFSNMAGQFSKIGQSLNASKIGRGSGGSKGKPGKGGADDAVAGGDGAAARSGGRRDGSSGSVSSFGGSVGAGGTTGGLRPGSASAGGKMERSSSESEEGACMYEPEDELVEQNPLYNENVFLPSVGIVMGGGGGAGEQEGVGSASSNVLEERDSIAKMSSDLSTMSISSVTDHINMPVGMLECASPIRVSSPAPEIFVDGTASGGDGGGGDGGPNRPGRNTMSLNLQAPAGGGGGGGGGSDNALRQLKKLTSPLSNIGKGLQTLGANLDPRKITVKSPVAPVRSTSAAFAEIKQLEEKWEKASCRTKLIAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V908</accession>
    <name>A0A6E8V908_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">mitogen-activated protein kinase kinase kinase</fullName>
            <ecNumber evidence="3">2.7.11.25</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.25"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.25"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.25" evidence="3"/>
    <dbReference type="RefSeq" id="XP_040237350.2">
        <property type="nucleotide sequence ID" value="XM_040381416.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040237358.2">
        <property type="nucleotide sequence ID" value="XM_040381424.2"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON000747-RA">
        <property type="protein sequence ID" value="ACON000747-PA"/>
        <property type="gene ID" value="ACON000747"/>
    </dbReference>
    <dbReference type="GeneID" id="120958527"/>
    <dbReference type="CTD" id="42366"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_013235"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000747"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038019"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004709">
        <property type="term" value="F:MAP kinase kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06624">
        <property type="entry name" value="STKc_ASK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000054">
        <property type="entry name" value="Mitogen-activated protein kinase kinase kinase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000487">
        <property type="entry name" value="Serine/threonine protein kinase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046872">
        <property type="entry name" value="DRHyd-ASK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046873">
        <property type="entry name" value="HisK-N-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043969">
        <property type="entry name" value="MAP3K_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025136">
        <property type="entry name" value="MAP3K_TRAF-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11584:SF394">
        <property type="entry name" value="APOPTOTIC SIGNAL-REGULATING KINASE 1, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11584">
        <property type="entry name" value="SERINE/THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19039">
        <property type="entry name" value="ASK_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20309">
        <property type="entry name" value="DRHyd-ASK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20302">
        <property type="entry name" value="HisK-N-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13281">
        <property type="entry name" value="MAP3K_TRAF_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="618"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="885"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1183"/>
            <end position="1207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1243"/>
            <end position="1296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1374"/>
            <end position="1405"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="203"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="918"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="1186"/>
            <end position="1195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="1248"/>
            <end position="1258"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="647"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000747-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1526" mass="171066" checksum="6519BE4C7FBB0D71" modified="2020-08-12" version="1">MSNLSDISGNTAVPAGAHAGPGRPRMDIAAVIDCEKSACLSHRKAALEEVQQACAAVGANLQLLPFDVLDYHNENNNKLEYFYNADVVVVDLSVQTQQSTLCYLLGVRESFKMKENIVIYNDTQTEATLRMKISCGNYQFLSYRLHENGGAIVTSPLKLEETAGTAAAASQLAQQPTTLAVALRRLFQDVEIQSKTHLREKFLADLRSLRDQHAGNVEELQRMLRNLRKRLDDPQVLSKDILQTYMLALRDVQDYDAMVQLVDDLQTIPNKQSYINTGHMHYLYAFALNRRNHEGDRERALRTCTKALEKKENHFPDMLCLYGRIYKDIFVESNHTDRESLKNAIIWYRKSFEVQPNEFAGINLATLLVIDGKDFTDSELQHIGMKLNNLIGKKGSLAQIKDYWNVATYFEISVLAENYAKAIQAAECMFKLRPPKWYLKSTIGNITLIDRARKRTEEPAGISIEQQIFQFWMEFFLEATAREPSSMVRFPILILEPQKIYMPSYVSIHMDAEQKSIDISNVCQQHAKGTCLKLHDFNFLASQIKSVSLYKRDERCAYLYVHHNSDDFQMFFPSVQCRQTFYDLILQMTADQGSGFIDLSAAETTADELKYEYDLDDEGQRILLGKGTYGAVYAARDLTTQVKIAVKEVYERNTHDVQPLHEEIKLHSQLRHRNIVQYWGSKSENQYFKIFMEQVPGGSLSALLSSKWGPLKDSETTIAFYSRQILEGLKYLHDQKIVHRDIKGGNVLVNTYSGVVKISDFGTSKRLAGINPATETFTGTLQYMAPEVIDQGVRGYGPAADIWSFGCTVVEMATGKPPFVELGCPQAAMFKVGFYKTHPTIPEELSSMAKNFILRCFEVNVDKRATATELLEDPFLCEKHKKMRPSFSSSSTISGLPHPGASEFSRSVSMPADRHNSKTLTTQESSASCNTPTFSSETEASSIPPLNATIGVQTASRSVTERKNKHSFQLTPIKMPKHGISSIGNLAETPSLEMDSAEQTSTVFQLNRRSSSGGLLSPEIDITTSTASKLPLAGDANESDGFYLLKKDSQRRQTLDKVLCHDVTKICELWLTKIEPDANQELAITRDHLVMLLNGLRKYIAEQRTDTLEQAIAQLKEQPNFNDIAIVQLHLALYAFQNTVITVLRSHNIKPHWMFALENLVKSAVHAGITILSPKLGANLAGNAPNDDEYDEESNELGGQAGPAPPAARAPLVNGRFAPAAARDERDGSAYDCEYDMYDLMAEGGGAGDEDPMDELDDPLTGTSSGHGTLRGMKPRARSHGTDVGPGTPLQKKTRWKTWREQVEALRAENQRLKEYLLESQQSYHGAFRAVLQSGAFARCLTDQLVALMERCLHEHGTGPELGDGEETFGDGAAAAGGGGRVRRTVQPWNRMPPVGQTATDRPTQCASDRVVATEDGQHVEEADEDEEMGDLRLAEWLQAQGIKRPECQRRILREGFLCDDFLYDLERDDLRRLGLKIGEELRIWNALKRHRRLHPPKRPSADCDSVHNINNNCLNDHHTAGLDTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V913</accession>
    <name>A0A6E8V913_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Peptidase M14 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">Ngousso</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the peptidase M14 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8V913"/>
    <dbReference type="EnsemblMetazoa" id="ACON000756-RA">
        <property type="protein sequence ID" value="ACON000756-PA"/>
        <property type="gene ID" value="ACON000756"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_003066"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000756"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035460"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006518">
        <property type="term" value="P:peptide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03858">
        <property type="entry name" value="M14_CP_N-E_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11308">
        <property type="entry name" value="Peptidase_M14NE-CP-C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000048">
        <property type="entry name" value="carboxypeptidase D isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1120:FF:000015">
        <property type="entry name" value="carboxypeptidase D isoform X4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1120">
        <property type="entry name" value="Carboxypeptidase-like, regulatory domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008969">
        <property type="entry name" value="CarboxyPept-like_regulatory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057246">
        <property type="entry name" value="CARBOXYPEPT_ZN_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057247">
        <property type="entry name" value="CARBOXYPEPT_ZN_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000834">
        <property type="entry name" value="Peptidase_M14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050753">
        <property type="entry name" value="Peptidase_M14_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11532:SF84">
        <property type="entry name" value="CARBOXYPEPTIDASE M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11532">
        <property type="entry name" value="PROTEASE M14 CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13620">
        <property type="entry name" value="CarboxypepD_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00246">
        <property type="entry name" value="Peptidase_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00765">
        <property type="entry name" value="CRBOXYPTASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00631">
        <property type="entry name" value="Zn_pept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49464">
        <property type="entry name" value="Carboxypeptidase regulatory domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00132">
        <property type="entry name" value="CARBOXYPEPT_ZN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00133">
        <property type="entry name" value="CARBOXYPEPT_ZN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52035">
        <property type="entry name" value="PEPTIDASE_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026355587" description="Peptidase M14 domain-containing protein" evidence="9">
        <location>
            <begin position="23"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M14" evidence="10">
        <location>
            <begin position="76"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="8">
        <location>
            <position position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS52035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000756-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="495" mass="55253" checksum="797DA200D46F6CB7" modified="2020-08-12" version="1">MQPAGLAVLLVAVWCHAVPAIADVQPEQRNFEPSTDPALFAFDRGAGDGGLYTFDNLSVAQDPRAYRPDVGALDFVYHNHEEMTRYLRATTARYPNLTALYSIGKSAQGRDLWVLVVSASPYEHMLGKPDVKYIGNIHGNEAVGRELLLHLIQYLVSSYGSDPYIKWLLDNTRIHILPSLNPDGYAASKEGTCDGGQGRYNSRGFDLNRNFPDYFKQNNKRSQPETEAVKDWISKIQFVLSGSLHGGALVVSYPYDNTPNAIFHSYLSQPSLTPDDDVFKHLSLIYANNHGKMSRGVACKTASPSFENGITNGAAWYPLTGGMQDFNYVWHGCMEVTLEVSCCKFPPAYELRKYWDDNQMSMIKFLAEAHRGVQGFIMDPTGNPVERAQLKIKGRDIGFTTTKYGEFWRILLPGVYKLEVYADGFVPKDVDFMIVEQHPTLLNVTLQPAKEAAEYSASQLPGASYDRPLTPTTPIVNREPARPNAANQDRLIFTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VAJ6</accession>
    <name>A0A6E8VAJ6_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphatidylinositol transfer protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_040241144.1">
        <property type="nucleotide sequence ID" value="XM_040385210.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040241146.1">
        <property type="nucleotide sequence ID" value="XM_040385212.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VAJ6"/>
    <dbReference type="EnsemblMetazoa" id="ACON001957-RB">
        <property type="protein sequence ID" value="ACON001957-PB"/>
        <property type="gene ID" value="ACON001957"/>
    </dbReference>
    <dbReference type="GeneID" id="120961488"/>
    <dbReference type="KEGG" id="acoz:120961488"/>
    <dbReference type="CTD" id="42306"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_003053"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001957"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_029514"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031210">
        <property type="term" value="F:phosphatidylcholine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008525">
        <property type="term" value="F:phosphatidylcholine transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008526">
        <property type="term" value="F:phosphatidylinositol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08888">
        <property type="entry name" value="SRPBCC_PITPNA-B_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.530.20:FF:000025">
        <property type="entry name" value="Phosphatidylinositol transfer protein beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001666">
        <property type="entry name" value="PI_transfer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055261">
        <property type="entry name" value="PI_transfer_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10658">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL TRANSFER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10658:SF11">
        <property type="entry name" value="VIBRATOR, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02121">
        <property type="entry name" value="IP_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00391">
        <property type="entry name" value="PITRANSFER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphatidylinositol transfer protein N-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001957-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="272" mass="31613" checksum="D57E39298F0A979B" modified="2020-08-12" version="1">MLIKEFRVTLPLTVEEYQVAQLYCVAEVSKNETGGGEGIEVLKNEPFDNYPLLGGKYNSGQYTYKIYHLASKVPAFIRLLAPKGSLEVHEEAWNAYPYCRTVITNPNWMKEKMTITIDTYSIDGDDDLENAHELTPEKLKQRDVVHIDIANDPVSQTDYRETEDPTKFKSKKTGRGPLVGPDWKKSVSPVMTCYKLVTCEFKWFGLQSRIENFIQKSERRLFTIFHRQVFCWMDSWHGLTMQDIRELEDKTKEELDKQRHVGEVRGMRAESD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VCB6</accession>
    <name>A0A6E8VCB6_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Chloride channel protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the chloride channel (TC 2.A.49) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VCB6"/>
    <dbReference type="EnsemblMetazoa" id="ACON002891-RA">
        <property type="protein sequence ID" value="ACON002891-PA"/>
        <property type="gene ID" value="ACON002891"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001039"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002891"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032829"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005247">
        <property type="term" value="F:voltage-gated chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03683">
        <property type="entry name" value="ClC_1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3080.10:FF:000042">
        <property type="entry name" value="Chloride channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.580.10:FF:000026">
        <property type="entry name" value="Chloride channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.580.10:FF:000032">
        <property type="entry name" value="Chloride channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3080.10">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014743">
        <property type="entry name" value="Cl-channel_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050970">
        <property type="entry name" value="Cl_channel_volt-gated"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001807">
        <property type="entry name" value="ClC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45720">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45720:SF10">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00654">
        <property type="entry name" value="Voltage_CLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00762">
        <property type="entry name" value="CLCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81340">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0129">CBS domain</keyword>
    <keyword evidence="8 10" id="KW-0868">Chloride</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="230"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="273"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="378"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="414"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="460"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="534"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="631"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="13">
        <location>
            <begin position="687"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="68"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1017"/>
            <end position="1058"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="747"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="90"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1017"/>
            <end position="1031"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1042"/>
            <end position="1058"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002891-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1058" mass="117768" checksum="FEA0D9087B7AF3B4" modified="2020-08-12" version="1">MHIPIKDEYVRDFSFEPEFILRRKDSLDIQREKYHRKRLNSRRKRLKRNALLVLAKTAAIVPPPLGHQHRVVEKNDPGEMPVRPDGVENGSRNSETSACLPTTSSSSSRSSTTNMHTSSRSNAMLIDVPSPDDCPAGSGLDDLDPADKDEIRRSIVDIEIEEFYYMYGRYTKDLGEYAKDEARRLRLLERRRKKDDKARNKELLDVRDNKFFKAASWLWRNTFARLGEDWVFLALLGIIMALLSYVMDKGISMCTNSRVWLYRDLTNHPVLQYLAWVSLPVCLILFSAGFVHLVAPQSIGSGIPEMKTILRGVALKEYLTFKTLVAKVIGLTATLGSGMPLGKEGPFVHIASIVSQLLSKIITSFQSIYENESRNTEMLAAACAVGVGACFAAPIGGVLFSIEVTTTYFAVRNYWRGFFAAVCGATVFRLLAVWFQKADTVTAMFSTNFTSDFPFDPQELFVFALIGRFLYPGIVALIVATLSFPLGFGKYIAGELSTHDQVHQLFSNFTWTKHELTVEQAAVVSNWRTPETNVFANLIIYLLYTFFMSIIASTIPVPSGMFIPVFKIGAAFGRIIGEAMHLWFPHGVRYGGMLAPIIPGGYSVVGAAAFSGAVTHTVSVGVIVFEMTGQITHIVPVMIAALISNAVAALLQPSMYDSIILIKKLPYLPDLLPSGSGMYNIYVEDFMVRDVRYIWKGISYQQLKEILKANKSLRSLPIVDSPDNRTLLGSVQRYELIKMIDKHIGREKRLEVAAKLMKEAEEKAREEQERQKREAAEAAKARRPSRFEVVPAPDILKLRERANNEMLPPQARRESNASLTSGPVFGATQPKKSILKKTNSFTLKGFSPLSPHSPVHTPYTTITGTESRIRSAFDIIFRKSATLQDVTPDPEIGSIGTPSIVGSEVAPLTPGISKKVQLPRERVIDMSPEDQKAWEMEEMAKPIDVGNLHIDPAPFQLVERTSILKVHSLFSMVGINHAYVTNVGRLVGVVALKELRTAIESVNNGTLTPETVKAKQEEEARALAQKGKESDNNNGISAEDPLLPKRPGDTFSDKKARN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VCB9</accession>
    <name>A0A6E8VCB9_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">K Homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_049462729.1">
        <property type="nucleotide sequence ID" value="XM_049606772.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VCB9"/>
    <dbReference type="EnsemblMetazoa" id="ACON002896-RB">
        <property type="protein sequence ID" value="ACON002896-PB"/>
        <property type="gene ID" value="ACON002896"/>
    </dbReference>
    <dbReference type="GeneID" id="120949698"/>
    <dbReference type="CTD" id="124204813"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011137"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002896"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040328"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22435">
        <property type="entry name" value="KH-I_NOVA_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22436">
        <property type="entry name" value="KH-I_NOVA_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09031">
        <property type="entry name" value="KH-I_NOVA_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000022">
        <property type="entry name" value="RNA-binding protein Nova-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047275">
        <property type="entry name" value="KH-I_NOVA_rpt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047276">
        <property type="entry name" value="KH-I_NOVA_rpt2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047274">
        <property type="entry name" value="KH-I_NOVA_rpt3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10288">
        <property type="entry name" value="KH DOMAIN CONTAINING RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="8">
        <location>
            <begin position="69"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="8">
        <location>
            <begin position="162"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="8">
        <location>
            <begin position="481"/>
            <end position="554"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002896-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="584" mass="61012" checksum="C7CA9B03599E0E53" modified="2020-08-12" version="1">MWPYGYPPEHNESKAMDAHLLTMFLQRKSPDAHIDPSRTVTQLNHAMELFEATGTTTATTTTATNGGGDNYHFKTLVPSVAAGAIIGKGGETIASLQKDAGARVKMSKAHDFYPGTTERICLISGTVEGILAVLDFITDKIREKPDITKALTEADAKQAQERDKQVKILVPNTTAGMIIGKAGAYIKQIKEESGSYVQISQKPKDLTLQERCITIIGEKENNRIACKMILAKIVEDPSSGTCLNVSYADINGPVANFNPTGSPFAASQNPNFSSSTASLNSAISGPLPNAAATGLLVNGTGLNLSLNLGAPSPQTNPTVTTQLLDHIKSAMRQSGYSDTATAEVHAALGVLAKYGVLGIGVGLQNGTHSTTLGYLGMPEMQQSSAAAAAAASAATGVYGAVGQLNLDSYLHGAGTATPRTTAIDRYDPTTFDPFRHPGTQAATPISINNNAFGLGNPSALNAAAQTLGSLSKSPTPAEMTTSKEKNVEIPEVIVGAILGPAGRSLVEIQHISGANIQISKKGIFAPGTRNRIVTITGQQNAINVAQYLIEERISQEETKRACQTAANAAATNKTSAASVATVTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VEF0</accession>
    <name>A0A6E8VEF0_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Saposin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VEF0"/>
    <dbReference type="EnsemblMetazoa" id="ACON001082-RA">
        <property type="protein sequence ID" value="ACON001082-PA"/>
        <property type="gene ID" value="ACON001082"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006880"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001082"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040829"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006665">
        <property type="term" value="P:sphingolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.225.10:FF:000002">
        <property type="entry name" value="prosaposin isoform X2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.225.10">
        <property type="entry name" value="Saposin-like"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003119">
        <property type="entry name" value="SAP_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007856">
        <property type="entry name" value="SapB_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008138">
        <property type="entry name" value="SapB_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008373">
        <property type="entry name" value="Saposin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011001">
        <property type="entry name" value="Saposin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008139">
        <property type="entry name" value="SaposinB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051428">
        <property type="entry name" value="Sphingo_Act-Surfact_Prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11480:SF3">
        <property type="entry name" value="BCDNA.GH08312"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11480">
        <property type="entry name" value="SAPOSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02199">
        <property type="entry name" value="SapA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05184">
        <property type="entry name" value="SapB_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03489">
        <property type="entry name" value="SapB_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01797">
        <property type="entry name" value="SAPOSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00162">
        <property type="entry name" value="SAPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00741">
        <property type="entry name" value="SapB"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47862">
        <property type="entry name" value="Saposin"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51110">
        <property type="entry name" value="SAP_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50015">
        <property type="entry name" value="SAP_B"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026302379" description="Saposin" evidence="7">
        <location>
            <begin position="22"/>
            <end position="1006"/>
        </location>
    </feature>
    <feature type="domain" description="Saposin A-type" evidence="9">
        <location>
            <begin position="33"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="Saposin B-type" evidence="8">
        <location>
            <begin position="77"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="Saposin B-type" evidence="8">
        <location>
            <begin position="191"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="Saposin B-type" evidence="8">
        <location>
            <begin position="298"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="Saposin B-type" evidence="8">
        <location>
            <begin position="446"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="domain" description="Saposin B-type" evidence="8">
        <location>
            <begin position="549"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="domain" description="Saposin B-type" evidence="8">
        <location>
            <begin position="684"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="domain" description="Saposin B-type" evidence="8">
        <location>
            <begin position="806"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="domain" description="Saposin B-type" evidence="8">
        <location>
            <begin position="895"/>
            <end position="976"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001082-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1006" mass="113083" checksum="5A3D37B2E4DDDF68" modified="2020-08-12" version="1">MKSRLLGLVCLVMLAINPVQSSPVKQTINGNKSLLGAKECTWGPSYWCSNIENAKTCAAVSHCIQTVWEKQHYPVDNDEICNICLDMVKQARDQLESNETQADLKAVFEGSCNLIPIKLVKKECRKLADDFIPELVEALASQMNPNAVCSVAGLCNNAAIDKLLSEMIVLNPEQEVQGIEANEREPAVTGDTFNCDECHTISNLIQKRFRSTDRDSVLESILQICGKMSSYSDACSNIVLTYFGVMYEHLERNLKVEGVCHLSGVCAAQFHLHENNKRKDLIEARSMGDVGRLRVAGDDVPCKLCEQLVDHLRDVLIANTTELEFKEVLEGLCNQTKSFADECHSLVEQYYREIYETLVNNLNSNDACFIIGICPKEDGVVSNGTVMPLLSVAVADRHERTEMYQRMNRPLLGHNELILSASEVQQAQLPIDRIMSAPSGLNLIENGKFCTLCEYFMHFVQEALSEPANEDEIKKVIEVTCNRLPSSIRGECHNFVDVYGDAVIALLIQSINPRQICPTLKMCPSASLDVEIFAPAPVEVSITARGDKDKPTCPLCLFAVSQLEESVKTDRSKENIKSALNKLCTHLSPKLRLECNDFVDTYTAELVEMLASDFTPQEMCVFLKLCVDQRPDLSLLGMELDYEKRSSHQKSDILVSSSGDIETNEIPDITVNGEITIDHTLMSSSPECLVCQEMVKEVEKRVQNKKSKEQIKQALEHACDRLRKYKTKCEKYIDQHSDQIIDLVMKQLSPKEICNALGFCIPAQEIIDDLIDEALFEYFGEDGVIKFTKDFLISSKRVDTGLEKNQPAQCAICEFVMVKLESELADNKTRTEIENAVRTVCDKMPGTITKQCDKLIDQYGEFIIKFLQTLPPKAICTQLDLCKEQLSRLKQSELEIVKCAVCHGAVKNLYELMAHDDIKGHIMNHVNSVCNSLQGDYFTLCEKLFTMYGIDMMYQLKHSIQKEYVCININMCSKSTLLTLPEFASDEHAVDQSFKARVENEETMLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VEF7</accession>
    <name>A0A6E8VEF7_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Glycoside hydrolase family 31 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the glycosyl hydrolase 31 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VEF7"/>
    <dbReference type="EnsemblMetazoa" id="ACON001111-RC">
        <property type="protein sequence ID" value="ACON001111-PC"/>
        <property type="gene ID" value="ACON001111"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002591"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001111"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037562"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06592">
        <property type="entry name" value="GH31_NET37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050985">
        <property type="entry name" value="Alpha-glycosidase_related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048395">
        <property type="entry name" value="Glyco_hydro_31_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000322">
        <property type="entry name" value="Glyco_hydro_31_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43053">
        <property type="entry name" value="GLYCOSIDASE FAMILY 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43053:SF6">
        <property type="entry name" value="SITS-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01055">
        <property type="entry name" value="Glyco_hydro_31_2nd"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21365">
        <property type="entry name" value="Glyco_hydro_31_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="51"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 31 TIM barrel" evidence="5">
        <location>
            <begin position="354"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 31 TIM barrel" evidence="5">
        <location>
            <begin position="525"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 31 C-terminal" evidence="6">
        <location>
            <begin position="641"/>
            <end position="723"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF21365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001111-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="730" mass="83456" checksum="12642AD19FCBBEAB" modified="2020-08-12" version="1">MMSNDFDTHSVLSNQASITSVNSLASLLKEKMQNVPAIIRKKKRETKDYKLRVFVGMLFLIIVFLVGYAYVMYNQKLLAKSYFESIKFHKKTRNFRLLDGKGGELLTGVLGTSLNIEQPYNCLPKDLKDDGSVCYEWNSKARLYMNLAKSPGPDIKCYSFQWESLLADLYPTDCFDVSEERGFWYGGGLTKGIEYILGKATIPAGPFVTGDINMHQWGNALKRYFLNSRGVAIQVDEKTPLYVSVNTDSGKRLCLQGKNDKFAFVNQQTRFPELKYTVCSGPDMKTLHNGLMQKSLWDGLTERDNDVIKSLLREPVWQIPASKQEDLTEVAIYNYTESIIALGYLRLGHVLVNEFWQRTIGDFRLDSERFPTLDETVNILHRRGFRVSFMIQPFISTESSNFREAVSKKLLIFERESERSIPALTRYKSTTSAGVLDVTNNASIPWLAEQLKKISETVEIDSYFVDFGTAFNIPRYYQCSQTLVNPDEYKNYFMERFEGTLSIFGVSSAISVPRPPAFLSLPPVNSSWSGLQSIIPTMLSYGIIGFPFLMPGPVGGDFVLPTQQLKKMYSYYSFELPPLPDKELYIRWLQLATFLPVLRFTHLPSEYRDETVTVIAKELADIRQRVLPLLERFSSIAMDEGLPIIRPLWMLDSTDVNCFSIDDEFSIGDGMIVAPVLKKGETVREVYLPQGVWKDGIDESLRKGSRWIHNYHVPQNKVAYFIKMPDNTRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VEF9</accession>
    <name>A0A6E8VEF9_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Gustatory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Gustatory receptor which mediates acceptance or avoidance behavior, depending on its substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the insect chemoreceptor superfamily. Gustatory receptor (GR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VEF9"/>
    <dbReference type="EnsemblMetazoa" id="ACON001117-RE">
        <property type="protein sequence ID" value="ACON001117-PE"/>
        <property type="gene ID" value="ACON001117"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_003504"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001117"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_036433"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042470"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
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        <property type="term" value="P:sensory perception of taste"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="7TM_chemorcpt"/>
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        <property type="entry name" value="GUSTATORY RECEPTOR 8A-RELATED"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="INVERTEBRATE GUSTATORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08395">
        <property type="entry name" value="7tm_7"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="42"/>
            <end position="63"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="8">
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            <begin position="83"/>
            <end position="101"/>
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            <begin position="147"/>
            <end position="167"/>
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    <feature type="transmembrane region" description="Helical" evidence="8">
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            <begin position="179"/>
            <end position="203"/>
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    <feature type="transmembrane region" description="Helical" evidence="8">
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            <begin position="298"/>
            <end position="318"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="338"/>
            <end position="361"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363108"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001117-PE"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="383" mass="43300" checksum="B437AC046012D08A" modified="2020-08-12" version="1">MSLFGEDALVAYETFSVLLKYLGVNPPISCDRKRTRLPYRCVNILSFVLSTLFYGYLLFSISLDSGVLKAGIKSLIVTRMHDLYFVLRYITVIVVQVHVLNNGGEINRLLRALNSISNAVTLLANRKGVSSKLRFFGTVRFAKGLRVFSLSYPFVCLAITFTILKWLNWNQLIGHVFQFVRLLYFTTYVHLWLQATLAAMLVLSRYEALKDLFGMYFPTSPSAVGNPSEFAICSEKEQINVLKQIKILHDKLNDVVELVNYCFSVQGKRIGILVHKAINCSSSSAVINEVQCCKGANMMLPVCNVPCAWIILILYLSRQLNLFSQQLLHRSPVITCGLFVYDWTLWYTMIGATATYLIILIQFDVSFPNLVNVNATAVYRGST</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VEI1</accession>
    <name>A0A6E8VEI1_ANOCL</name>
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        <recommendedName>
            <fullName evidence="3">CCDC174 alpha/beta GRSR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VEI1"/>
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        <property type="protein sequence ID" value="ACON001231-PA"/>
        <property type="gene ID" value="ACON001231"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000493"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001231"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031758"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025066">
        <property type="entry name" value="CCDC174-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057464">
        <property type="entry name" value="CCDC174_GRSR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15885">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 174"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15885:SF1">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 174"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25449">
        <property type="entry name" value="CCDC174_GRSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13300">
        <property type="entry name" value="DUF4078"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCDC174 alpha/beta GRSR" evidence="3">
        <location>
            <begin position="155"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="27"/>
            <end position="81"/>
        </location>
    </feature>
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            <end position="246"/>
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    </feature>
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            <begin position="503"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="41"/>
            <end position="66"/>
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    </feature>
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            <end position="150"/>
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            <begin position="222"/>
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    </feature>
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            <begin position="296"/>
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        </location>
    </feature>
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        <location>
            <begin position="372"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25449"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001231-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="588" mass="66980" checksum="E6F62C8D43806956" modified="2020-08-12" version="1">MNDPNRKIEIDKSSLLSLKAELLRKQEEVGRAKASTSIDDFVPKKVPKPEKDVSAKRKERREKKDSSQPVVTELEDSAQLARSKQMLVAKAKYYDRMVAAGGSLNSDENSLVMFNKKKQDTKPVYSSSEHSAEESSTDSESSDSDSEGVEDGDKWVEYTDCLGRTRKCLKKDLKECLARDKELAKSMAPREGQRREDLQGSSSVHTVPLARPETLPSTSTDAQEEEDEDGEIVGPMPPAMSRTDDTDLGERFLEMKEQWAQQEEANLEKGSIHYQDVLFNEARLHGVGYYAFSTDQEERNRQRDELDSIRETTMEAQKEREALRQARDKLIADRVKAARARQRARQGLPPEEDEDSSKQQQTEASDQLYDPVEERRKRKAEEKQRKRKEKEERRRERERADHVRPWDEGKEQNPDDKEWVPAKEKFILSQHEWNDLKRSERIAEFAPPPVETASTARSRRSLFFTTKKNPPKKELKRRNLNAVEMSTPAKMAAPVPIRNELSDGEADEEAAVLPPRKGAEIEPPATYDYYGPSDAARRSRPAAGQTVAPENRTNLEASIEAGLKFLRNQSDKSGGPGTKNRWTSNADY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VEI9</accession>
    <name>A0A6E8VEI9_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="14">C2H2-type domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VEI9"/>
    <dbReference type="EnsemblMetazoa" id="ACON001269-RB">
        <property type="protein sequence ID" value="ACON001269-PB"/>
        <property type="gene ID" value="ACON001269"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_008853"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001269"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041873"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001156">
        <property type="entry name" value="Zinc finger protein 407"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050752">
        <property type="entry name" value="C2H2-ZF_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24384:SF189">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24384">
        <property type="entry name" value="FINGER PUTATIVE TRANSCRIPTION FACTOR FAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="407"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="435"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="463"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="491"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="71"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="165"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="250"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="321"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="520"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="641"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="168"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="205"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="250"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="262"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="325"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="373"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="526"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="641"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="666"/>
            <end position="691"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001269-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="691" mass="74437" checksum="B06E6C88CA1C5474" modified="2020-08-12" version="1">MQFLRPVEGTAVSAASAMFHQQQSQHGQIVTTAGMPHPGMASPAYITLPITMPGAKPGDAQQTVQIQVLNPNPVPSQVSGSAAGGQGQTGSTPSGALQTPKFQMGQMQIPIQGFQQGTTVLTVAYSPQDEEVLHNQGLPEGMTIVAALQPQDLQLLASQNPGTTTILQQHQQQQQQSNLSSNNASSPGGIKIDSENPPPLRQIKQESPTIWNQPTLVPTSMHSASNILEMVQQQQQQQQQLHQLQQHQQQQQQQQQQQQQQPSRPTQNLNLQPYLKFNGGNNGTNPAMTTLMNGNHVLQQLVLPPGVTIGPGIIKQEVPDEHSNENTQFGQADSSGQLENGSPNARPANGREAEGEPGENGDGTGNGTTPSGKARKKRKYKTKPPKPKRQKPGQVHIATAIDGTVLFCCPECHMAYPEKECLEQHLVVHKIERRFICDICGAGLKRKEHLERHKLGHNPERPFICNVCEKGFKRKEHLNLHFVIHSGVKTEICDDCGKGFYRKDHLRKHIKSHLSKRLKEMRDTNRNNNNNNSNSNNRNTTNGATTTTTTTTTPAMANGPPSTSIATLSTGIPPGLTITTSSGNNMQSATGEGIGESNPTAQNLLHSAISLPQGVTIHVPTADNQTLPVQISLPHLVAQQQTDTDGSTRTVLVSNSPENLNHHHGATTTTTTTTATTGTGSPPSAPTSSAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VEU0</accession>
    <name>A0A6E8VEU0_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Oxidation resistance protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the OXR1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040223486.1">
        <property type="nucleotide sequence ID" value="XM_040367552.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040223487.1">
        <property type="nucleotide sequence ID" value="XM_040367553.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040223489.1">
        <property type="nucleotide sequence ID" value="XM_040367555.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_049463439.1">
        <property type="nucleotide sequence ID" value="XM_049607482.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VEU0"/>
    <dbReference type="EnsemblMetazoa" id="ACON001751-RC">
        <property type="protein sequence ID" value="ACON001751-PC"/>
        <property type="gene ID" value="ACON001751"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON001751-RD">
        <property type="protein sequence ID" value="ACON001751-PD"/>
        <property type="gene ID" value="ACON001751"/>
    </dbReference>
    <dbReference type="GeneID" id="120949921"/>
    <dbReference type="CTD" id="45467"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005654"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001751"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034408"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006571">
        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354:SF62">
        <property type="entry name" value="MUSTARD, ISOFORM V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354">
        <property type="entry name" value="NUCLEOLAR PROTEIN 7/ESTROGEN RECEPTOR COACTIVATOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07534">
        <property type="entry name" value="TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00584">
        <property type="entry name" value="TLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51886">
        <property type="entry name" value="TLDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TLDc" evidence="5">
        <location>
            <begin position="51"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00040604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001751-PD"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="212" mass="23998" checksum="E4367DE431379C0D" modified="2020-08-12" version="1">MMSKSKINKLTKYLKTKVLSMSTDDYRKATLFASGSFDQDFQIPDLVGQTEILSEEHREKLCAHLPARAEGYSWSLVFSTSQHGFSLNSLYRKMHKLESPILIVIEDTDHNVFGALTSCSLHVSDHFYGTGESLLYKFNPHFKVFHWSGENLYFIKGNPESLAIGAGDGKFGLWLDGDLNQGRSQHCSTYSNEPLAPQEDFVIKTLECWAFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VEU6</accession>
    <name>A0A6E8VEU6_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Extremely potent competitive inhibitor of cAMP-dependent protein kinase activity, this protein interacts with the catalytic subunit of the enzyme after the cAMP-induced dissociation of its regulatory chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PKI family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040222601.1">
        <property type="nucleotide sequence ID" value="XM_040366667.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VEU6"/>
    <dbReference type="EnsemblMetazoa" id="ACON001779-RA">
        <property type="protein sequence ID" value="ACON001779-PA"/>
        <property type="gene ID" value="ACON001779"/>
    </dbReference>
    <dbReference type="GeneID" id="120949408"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010923"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001779"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038127"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004862">
        <property type="term" value="F:cAMP-dependent protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004171">
        <property type="entry name" value="cAMP_dep_PKI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15416">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE INHIBITOR/PKI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02827">
        <property type="entry name" value="PKI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0649">Protein kinase inhibitor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="75"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001779-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="110" mass="11073" checksum="09D4C93C637B3999" modified="2020-08-12" version="1">MDKLRESGAADDPTTQQRLATDGQGGTAAAAGGEGTSDEYHHQFYNTGRIGRRNALPDILGTHCTTTTADLSSQLGSLSTSDCAGKASDGSSSCIAAGNNGQPPPTTTAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VF34</accession>
    <name>A0A6E8VF34_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">GATA-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VF34"/>
    <dbReference type="EnsemblMetazoa" id="ACON002238-RB">
        <property type="protein sequence ID" value="ACON002238-PB"/>
        <property type="gene ID" value="ACON002238"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006533"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002238"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039052"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045165">
        <property type="term" value="P:cell fate commitment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00202">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000032">
        <property type="entry name" value="Transcription factor GATA-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039355">
        <property type="entry name" value="Transcription_factor_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10071:SF337">
        <property type="entry name" value="GATA-BINDING FACTOR A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10071">
        <property type="entry name" value="TRANSCRIPTION FACTOR GATA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00320">
        <property type="entry name" value="GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00619">
        <property type="entry name" value="GATAZNFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00344">
        <property type="entry name" value="GATA_ZN_FINGER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GATA-type" evidence="11">
        <location>
            <begin position="770"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="25"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="146"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="206"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="323"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="383"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="450"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="518"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="814"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="878"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1067"/>
            <end position="1115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="26"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="81"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="156"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="323"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="398"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="477"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="524"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="830"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="883"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="905"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1106"/>
            <end position="1115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002238-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1115" mass="118756" checksum="C86B4D02697AA9A7" modified="2020-08-12" version="1">MPGKDCYEPSFGAIVGAYKSPVLVSPQAGAQQHPQEQQPQQQGIDNGGKQAADDSEPPDTGIIGPGARPVSERASPGASRAESDSADHRAREADDREATVPDASATHDTLTGGGTAEPGATSAASGNTPSVITSCSKVLLGTDPVKGPAYGAEQPLQKQEASPGSSSPPRFSTLQTVINSSTGVGGYPAAYQHHWPCDLTYKPVDHRPVSSPSSEPSAGELTRHPTPSRGAYIGEPDVASSPSQGHLAVVSHLNPLVCSTADPSLLGGMRGNLLSMYSSYASPLGGGPHHQHDHLDEATALHHHVQAQDAHLRGDDEMSAIHHHLHQQHHHQQQQQQQHHAQHHHHHHDVVAQQHSIDDMIADTLKDEQCGMVDSYLTPTLVSDHHHHLGDSPTHHNVQQHHHHHQQQQHLSQHHLQQHGMHESKEYASMYHNNNDKSVINYNHAATIAAAHHQQQQQSDHHQQNTHTHTSSGGDSRSPEYSHAHDEYDGGLQSFTQLINVPRASDTAASMYHHRHPMSAESDAGTAAGATPNGGQTPTTTNTATSSPGPADHLVQLGGTGPQAGGPGAVLLHSVENGTGAQEPGTVTGTDGSISLYDTLHTSVLAGGPSYGRCSFPSMQYFNGNPTDHLWSSGVSGLENDYMKGSLPAFQRIVSGTNTSRTNPYSAVSTSYAQQQNDTWSQHYDTGSIAYSVATSSTTPVTVNRRAAVAAAAAAAAVATTTTTHFPAAASLTALAAEQGGDLYKNNYTFSNLTTRTTEEKQSRRMSSARRVGLQCSNCNTTNTSLWRRNQVGEPVCNACGLYYKLHNVNRPLAMKKDNIQSRKRKPKGSKNSDGNGKSNASNASANRQTNSSSSSLAETPKKTDGLKLMNIVENSSFDKLLPSSPSSEGSNQSPAHHNHMSPICYTQQVPSPITSTSSSGAIVSNSKYNNQQPKATNAFLLQSPTPMNMFVGSPTGGIGSVQHHHHQHHQSTAAATTGALSPVSYSMAGVGHGNNNGSIVSSKYQQSPPQSPEDMLYYDMLPADVNGSVDQHSLGTIVKMEPMGGNHYASYQQALHHEGHMVGTLGHGQHAPGAHNHSRSPSVADEESEQAHGQQDMIESKHNINRPTVVSMTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VF54</accession>
    <name>A0A6E8VF54_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">STAS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_040219952.2">
        <property type="nucleotide sequence ID" value="XM_040364018.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VF54"/>
    <dbReference type="EnsemblMetazoa" id="ACON002331-RB">
        <property type="protein sequence ID" value="ACON002331-PB"/>
        <property type="gene ID" value="ACON002331"/>
    </dbReference>
    <dbReference type="GeneID" id="120948048"/>
    <dbReference type="CTD" id="42962"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_014001"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002331"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030882"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07042">
        <property type="entry name" value="STAS_SulP_like_sulfate_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.750.24:FF:000028">
        <property type="entry name" value="Sulfate transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.24">
        <property type="entry name" value="STAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011547">
        <property type="entry name" value="SLC26A/SulP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001902">
        <property type="entry name" value="SLC26A/SulP_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11814">
        <property type="entry name" value="SULFATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01740">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00916">
        <property type="entry name" value="Sulfate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52091">
        <property type="entry name" value="SpoIIaa-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="101"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="129"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="176"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="256"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="294"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="358"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="439"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="462"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="493"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="domain" description="SLC26A/SulP transporter" evidence="6">
        <location>
            <begin position="103"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="domain" description="STAS" evidence="7">
        <location>
            <begin position="545"/>
            <end position="627"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002331-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="655" mass="70263" checksum="5945CBAB035E513A" modified="2020-08-12" version="1">MKTQIHTQLGAPPHDGSYDNEALSCSTIDITAPNSGEPHGGLRGSNEFILSDSGKKVTGDDQTGQLNMWLQRKARSAFSEKMIKKRLPILRWLPQYNSTDAIGDLVAGITVGLTVIPQALAYAGIAGLPAAYGLYGSFLGCIVYIVFGSCKDVPMGPTAIASLLTFQTAGGNVGKAILLCFLSGVVELLMGLLGLGFLVDFVSGPVSSGFTSAVSLIIVTSQVKDVLGISAKGTTFIEIWRNIFNDIHNVQVWDTFLGLTCIAVLLIMRIIAGLKVGPADDELKSKNHRIINKLMWLIGTSRNAILVVVCGAIGYSFQSSAVAPFKLIGDIPPGLPSLKAPPFSLTANETMSGTEESFMEMVSSLGSGLIVVPLIALMENIAICKAFSNGKPVDATQELIAIGLANIANSFVQGFPGTGSLSRSAVNNASGVRTPLGNIYTMALVVLSLLFFTPYFSYIPKASLAAVIIAAVVFMVEVKVVKPMWRTKKSDLIPGLGTFIACLALPLEMGILFGVGLNVVFILYHAARPKISVERLTSPGGAEYLIITPDRCLIFPSVDYVRNLVTKQSIRQSLPVVIDCSHVYGADFTAATVIDSITQDFARRDQPLFFYNLKPSVCAIFEGLSPADFVVYYREEHLDDLLKQRAYKPKEVISA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VF58</accession>
    <name>A0A6E8VF58_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Troponin T</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the troponin T family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040222827.1">
        <property type="nucleotide sequence ID" value="XM_040366893.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_049462970.1">
        <property type="nucleotide sequence ID" value="XM_049607013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VF58"/>
    <dbReference type="EnsemblMetazoa" id="ACON002350-RB">
        <property type="protein sequence ID" value="ACON002350-PB"/>
        <property type="gene ID" value="ACON002350"/>
    </dbReference>
    <dbReference type="GeneID" id="120949532"/>
    <dbReference type="CTD" id="136032042"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011848"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002350"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041535"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005861">
        <property type="term" value="C:troponin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005523">
        <property type="term" value="F:tropomyosin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006936">
        <property type="term" value="P:muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006937">
        <property type="term" value="P:regulation of muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045214">
        <property type="term" value="P:sarcomere organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.350:FF:000003">
        <property type="entry name" value="Troponin T isoform 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027707">
        <property type="entry name" value="TNNT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001978">
        <property type="entry name" value="Troponin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038077">
        <property type="entry name" value="Troponin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11521">
        <property type="entry name" value="TROPONIN T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11521:SF1">
        <property type="entry name" value="TROPONIN T, SKELETAL MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00992">
        <property type="entry name" value="Troponin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90250">
        <property type="entry name" value="Troponin coil-coiled subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="40"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="202"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="277"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="13"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="40"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="60"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="112"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="139"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="202"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="294"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="306"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002350-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="361" mass="43418" checksum="7E3E74FAA60E64D1" modified="2020-08-12" version="1">MSDDEEYSGDDPEFIKRQDQKRSDLDEQLKEYINEWRKQRAKEEDELKKLKDKQAKRKVSRAEEEQRMAQRKKEEEERRVREVEEKKQREIDEKRKRLEEAEKKRQAMLQAMKDKDKKGPNFTITKKDSSFGMSNAQMERNKTKEQLEEEKKISLSFRIKPLEVDGLSADSLRARATELWETIVKLETEKYDLEERQKRQDYDLKELKERQKQQLRHKALKKGLDPEALTGKYPPKIQVASKYERRVDTRSYDDKKKLFEGGFDTLNKEVLEKQWAERKEQFGGRQKSKLPKWFGERPGKKSGDPETPEGEDEVKPDDEEVEEVEEVVEEVVEEEEEEEEEEEEEEEEEEEEEEEEEEEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VGJ3</accession>
    <name>A0A6E8VGJ3_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Alpha-catenin</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell junction</location>
            <location evidence="3">Adherens junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the vinculin/alpha-catenin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_049461655.1">
        <property type="nucleotide sequence ID" value="XM_049605698.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VGJ3"/>
    <dbReference type="EnsemblMetazoa" id="ACON003424-RB">
        <property type="protein sequence ID" value="ACON003424-PB"/>
        <property type="gene ID" value="ACON003424"/>
    </dbReference>
    <dbReference type="GeneID" id="120953521"/>
    <dbReference type="CTD" id="40517"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006798"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003424"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031991"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016342">
        <property type="term" value="C:catenin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008013">
        <property type="term" value="F:beta-catenin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045296">
        <property type="term" value="F:cadherin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.230:FF:000020">
        <property type="entry name" value="Catenin alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.230:FF:000007">
        <property type="entry name" value="Catenin alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.230:FF:000011">
        <property type="entry name" value="Catenin alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2510">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.230">
        <property type="entry name" value="Alpha-catenin/vinculin-like"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036723">
        <property type="entry name" value="Alpha-catenin/vinculin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001033">
        <property type="entry name" value="Alpha_catenin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006077">
        <property type="entry name" value="Vinculin/catenin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000633">
        <property type="entry name" value="Vinculin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18914">
        <property type="entry name" value="ALPHA CATENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18914:SF9">
        <property type="entry name" value="CATENIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01044">
        <property type="entry name" value="Vinculin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00805">
        <property type="entry name" value="ALPHACATENIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47220">
        <property type="entry name" value="alpha-catenin/vinculin-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00663">
        <property type="entry name" value="VINCULIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="8" id="KW-0965">Cell junction</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="869"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="869"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="890"/>
            <end position="909"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003424-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="909" mass="102043" checksum="C29511817890A341" modified="2020-08-12" version="1">METLSNFGQVALKWDPKNLEIRTMSVEKTLEPLVLQVTTLVSTKGPSKKKKGKSKRASALVAAVEKATDIFIERGEQIAYENPDITQEMLSAVEEVRKTGSAMSIAAREFSEDPCSSLKRGNMVRAARNLLSAVTRLLILADMVDVHLLLKSLHVVEDDLDKLRNASSQDELMNNMRQFGRNANELIKQAAKRQQELKDPQLRDDLAAARAVLKKHSTMLLTASKVYVRHPELDLAKVNRDHILKQVCEAVNTISDVAQGKSSQPQDVYVGAGELAAALDDFDEGIIMDPRAYNEVRSRPSLEERLESIISAAALMADADCTRDERRERIVGECNAVRQALQDLLSEYMSNMGTKDRTPELERAIGHMYRKTKDLRRQLRKAVVDHVSDSFLETNMPLLDLIDAARGGNEKLVRERADIFTKHAEKLVEVANLVCSMSNNEDGVKMVRYAADQIETLCPQVINAALILAARPNSKVAQENMEAYRQAWENQVRILTEAVDDITTIDDFLAVSENHILEDVNKCVLALQEGDAQDLRNTAGAIQGRSARVCNVVEAEMDNYEPCIYTKRVLEAVKVLREQVMSKFAQRVDVAVDALSSNSPKDVDENDFIDASRLVYDGVREIRRAVLMNRDEEDLDPEDIEDEQYTLETRSKSSAHTGDQTIDEYPEISGITTAREAMRKMNEEDKQKIMQQVELFRREKLTFDSEVAKWDDTGNDIIYLAKHMCMIMMEMTDFTRGRGPLKTTMDVINAAKKISEAGTKLDKLTREIADQCPESSTKKDLLAYLQRIALYCHQIQITSKVKADVQNISGELIVSGLDSATSLIQAAKNLMNAVVYTVKYSYVASTKYTRQGTVSSPIVVWKMKAPEKKPLVRPEKPEEVRAKVRRASQKKTQNPVHALSEFQSPTDAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VGK4</accession>
    <name>A0A6E8VGK4_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Transcription factor grauzone</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VGK4"/>
    <dbReference type="EnsemblMetazoa" id="ACON003565-RB">
        <property type="protein sequence ID" value="ACON003565-PB"/>
        <property type="gene ID" value="ACON003565"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012498"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003565"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035606"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
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        <property type="evidence" value="ECO:0007669"/>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
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        <property type="entry name" value="AT_hook_DNA-bd_motif"/>
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        <property type="entry name" value="Znf_AD"/>
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        <property type="entry name" value="ZnF_C2H2-type_TF"/>
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        <property type="entry name" value="Znf_C2H2_sf"/>
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        <property type="entry name" value="Znf_C2H2_type"/>
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        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR CTCFL-RELATED"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
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    </dbReference>
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        <property type="match status" value="12"/>
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    </dbReference>
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        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="5"/>
            <end position="81"/>
        </location>
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        <location>
            <begin position="772"/>
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        </location>
    </feature>
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        <location>
            <begin position="94"/>
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        </location>
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            <begin position="128"/>
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            <begin position="170"/>
            <end position="209"/>
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        <location>
            <begin position="223"/>
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        <location>
            <begin position="100"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="197"/>
            <end position="207"/>
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        <location>
            <position position="7"/>
        </location>
        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
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        <location>
            <position position="10"/>
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        <source>
            <dbReference type="PROSITE" id="PS50157"/>
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        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="819" mass="93858" checksum="5B121130D3F110AB" modified="2020-08-12" version="1">MAHITSCALCLTAESQWFVRVFDEANTTLNMAQIISKYLWFDVSRGMFLLPCVCGECWSSVHDFHQFYTKLENIHCDELVAADSTEQQRKPNASIALLPEETHQKEDQLQKSIDDGYERKHVPTVEVIDSSEKKNNTDSAANEPVKPHTQTDAMDTMDKGDKKHFVEVEDYASNDDDTGGYCPSDDNMLDGDTLNTEGRDESIDKKHTMNNVTVVAIPVEQLSVKRGRGRPPKPKLEQQSIAVDAKESPKDTSISEQTPAKRPRGRPPKRKLDISSASPNVAQKSEAPPAVIETSLVATTTEPVKRRRGRPPKNALTAVAVHSGRGRKVHSVTGNKRSIALRTKVKKEIIRGQEPTYTDSGESESENGSDYEAASAKKKPPKREKSDRDERIFKYVDEFCCHYCVENVTFKRFVDADRHYKLVHNEPGFLKCTKCEKKCFTPGMFVSHMETHEDPEKYKCNICGKITDCNISLKKHMRVHLSQLEENLPFPCSRCKRKFESEEQRNKHEKLHVPKPLVKREKGPDLEVLAFYKRIYCDVCEEQTPDSTSFENFWDLRVHMEQEHNKGAYLKCPICNKRNLCEVCKEVYQNLDSHMVKAHTPTTAIPADRKYKCDQCGRMFNFLANLKMHIDCVHGSKDIRCNVHKRTAHTDQMLMCEHCPKMFKTRGALEVHKGIHDDTLVQFTTCKLCNKQIRLTNVKKHMTSQHSDDGPVSCEMCGKSFRSMFHMKRHQKNTCEATIDSRKHKCEICGKGFCLKLTMIEHMTTHTRTNKYQCAFCFKSFGYISNLYKHRKKAHPLEWQEIQARPEENIASVIVVRTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VGK7</accession>
    <name>A0A6E8VGK7_ANOCL</name>
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        <recommendedName>
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        </recommendedName>
    </protein>
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        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
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            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
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        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VGK7"/>
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        <property type="gene ID" value="ACON003431"/>
    </dbReference>
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    <dbReference type="VEuPathDB" id="VectorBase:ACON2_036733"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="RUN_RUFY1_like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Uncharacterized protein, isoform B"/>
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        <property type="entry name" value="Run_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45956">
        <property type="entry name" value="RUN AND FYVE DOMAIN-CONTAINING PROTEIN 2-LIKE PROTEIN"/>
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    </dbReference>
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        <property type="entry name" value="RUN DOMAIN-CONTAINING PROTEIN"/>
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    <evidence type="ECO:0000256" key="3">
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    </evidence>
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    </evidence>
    <sequence length="602" mass="65358" checksum="87CD68A33C80C618" modified="2020-08-12" version="1">MRALGIAQPNNHHQTTVASNNTTTSTSSSSSTTASNVGSVGASTGGSAAAGINGKNGNGDGHHHGPGATLITLPRAIPAGTGSAGDGHHRLPGGQLRTAGHGGVVAGGGSVSDTAAQDTIYLCNFRVTVDGEWLCLKELQDLDIKDGQAGGGGGGSGGADALGGGGGGAGGGTSDCNALNSFGIIENVKCEEKIIERDWVNYYSRDPASIERSNLVKICKLVVKELLEQSLRFGRMLDSDHLPLQHFFIVLEHVLRHGLKPKKGLLGPKKELWDILQSVERYCVEAMDITSSVRDLPTVRTHMGRARAWLRLALMQKKLADYLQVLIEHRDDSLAEYYEPHALMLSDEIIIIVGILVGLNVIDCNLCVKEEDLDSQQGVIDFSLYLRSSSRNSPDEENNGGVGGGINDSATGAMTAVLDQKNYIEELNRHLNATVANLQAKVESLTTTNALMKEDLAIARNSLMALQAENAALRRASQHGHPIEDKTNVLDRFDPEMAETLAEERKKRQEIEKELDLQVSLKAETEMAMKLLEKDIHEKQDTIVSLRRQLEDIKSINLDMYRKLQVISGLWVWELWGYTGLYHKSLSKHLDHSGRDCYFFLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VGM0</accession>
    <name>A0A6E8VGM0_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="8">BTB domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VGM0"/>
    <dbReference type="EnsemblMetazoa" id="ACON003439-RE">
        <property type="protein sequence ID" value="ACON003439-PE"/>
        <property type="gene ID" value="ACON003439"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005866"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003439"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034718"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18315">
        <property type="entry name" value="BTB_POZ_BAB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000036">
        <property type="entry name" value="Mod(Mdg4), isoform H"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051095">
        <property type="entry name" value="Dros_DevTransReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007588">
        <property type="entry name" value="Znf_FLYWCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110">
        <property type="entry name" value="BTB DOMAIN TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110:SF92">
        <property type="entry name" value="MODIFIER OF MDG4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04500">
        <property type="entry name" value="FLYWCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="7">
        <location>
            <begin position="32"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="118"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="192"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="287"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="394"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="192"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="222"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="294"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003439-PE"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="541" mass="59540" checksum="7D96F76C78D7EB14" modified="2020-08-12" version="1">MADDEQFSLCWNNFNSNLSAGFHESLQRGDLVDVTLAAEGHLVKAHRLILSVCSPYFRKMFTQVPVNQHAFIFLKDVSHSALQDLIQFMYCGEVNVKQDALPAFISTAEALQIKGLTETGDSAPTHQSPAKEEPAAAAAVPVTTATISTATIPASPASQRAKVQRNRIQSYKLESEESGDDKVVHIQATTSHHVSAQSNLSSQKRTMPQRGLQSHASKRTKMSISASSDGLDTSDSTPAQVQTVQTVQIVKQIPAQVIEPEYIELPIESINPKAEPDYTDETAEIETVDAETEQEQKLSEHDQGDADDDGNYVEDDTYGDMAMGKYEESYLTEGEEGAKPGVSGFVDSYTSDGGNATEISTQENYVMLLTLMSYSFFRFAFSTAINRMLANTFSPPKESPSKQSQDSLRSSTVPAIANAPSGVKKHSTPISTNCRTISPKQCSKPVVNFAEITKTKPVEMSILPTRKGAAGVLCQGHHFEFRYTRQHHKVYRCAWHSTHSCQAQVLLHNKLFYIIHDKHTHSESSKLHPDILGKTTLKTNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VGM5</accession>
    <name>A0A6E8VGM5_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="8">BTB domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_049461687.1">
        <property type="nucleotide sequence ID" value="XM_049605730.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_049461688.1">
        <property type="nucleotide sequence ID" value="XM_049605731.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VGM5"/>
    <dbReference type="EnsemblMetazoa" id="ACON003439-RJ">
        <property type="protein sequence ID" value="ACON003439-PJ"/>
        <property type="gene ID" value="ACON003439"/>
    </dbReference>
    <dbReference type="GeneID" id="120949302"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005866"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003439"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034718"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18315">
        <property type="entry name" value="BTB_POZ_BAB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000036">
        <property type="entry name" value="Mod(Mdg4), isoform H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051095">
        <property type="entry name" value="Dros_DevTransReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007588">
        <property type="entry name" value="Znf_FLYWCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110">
        <property type="entry name" value="BTB DOMAIN TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110:SF92">
        <property type="entry name" value="MODIFIER OF MDG4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04500">
        <property type="entry name" value="FLYWCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="7">
        <location>
            <begin position="32"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="118"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="192"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="287"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="192"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="222"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="294"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003439-PJ"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="478" mass="52944" checksum="193E53D96BBFCB9A" modified="2020-08-12" version="1">MADDEQFSLCWNNFNSNLSAGFHESLQRGDLVDVTLAAEGHLVKAHRLILSVCSPYFRKMFTQVPVNQHAFIFLKDVSHSALQDLIQFMYCGEVNVKQDALPAFISTAEALQIKGLTETGDSAPTHQSPAKEEPAAAAAVPVTTATISTATIPASPASQRAKVQRNRIQSYKLESEESGDDKVVHIQATTSHHVSAQSNLSSQKRTMPQRGLQSHASKRTKMSISASSDGLDTSDSTPAQVQTVQTVQIVKQIPAQVIEPEYIELPIESINPKAEPDYTDETAEIETVDAETEQEQKLSEHDQGDADDDGNYVEDDTYGDMAMGKYEESYLTEGEEGAKPGVSGFVDSYTSDGGNATEISTQGAPKTRDDGNIQRACFEFTTRGTQCLVYDGYLYSKNKTFENGTRVNWKCRFYHRLHCKARAQTRLIDGVEYVKVFKNEHTHPQEAKSVRRRKRKIKLEPMMDQDQTAAHLAGLQLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VGV7</accession>
    <name>A0A6E8VGV7_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="29">Terribly reduced optic lobes</fullName>
        </recommendedName>
    </protein>
    <organism evidence="27 28">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="27">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endomembrane system</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
            <location evidence="2">Extracellular space</location>
            <location evidence="2">Extracellular matrix</location>
            <location evidence="2">Basement membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON003656-RB">
        <property type="protein sequence ID" value="ACON003656-PB"/>
        <property type="gene ID" value="ACON003656"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009102"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003656"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035234"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005604">
        <property type="term" value="C:basement membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00055">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00110">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002299">
        <property type="entry name" value="AGAP003656-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002808">
        <property type="entry name" value="AGAP003656-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000254">
        <property type="entry name" value="AGAP003656-PB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000236">
        <property type="entry name" value="AGAP003656-PC"/>
        <property type="match status" value="1"/>
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        <location>
            <begin position="3259"/>
            <end position="3269"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="3280"/>
            <end position="3289"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="3512"/>
            <end position="3529"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="3531"/>
            <end position="3540"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="3702"/>
            <end position="3729"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS51115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003656-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="29">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="3741" mass="415885" checksum="71C7537B24B13D5D" modified="2020-08-12" version="1">MFSANSRAALWLAVLLVVSIERASGQECTSNEFTCDDGRCIDQDRQCDGVADCSRGEDEQDCGIVECDESREFLCRSDNTCIQKEAVCDGNRDCSDGEDEICEAQRDKCSIYEFQCRWDKRCIPIEKRCDKVYDCLDRTDEQVCDCTAHEFRCDNGFCIPMYQRCDRVNHCNDRSDERGCNFTSRCSEHEFHCNNGNCIPKDYMCNDIDDCGDNSDETDSVCGDYTCMPDEFTCYDGGCVSKALVCDDRKDCEDGTDEMDCDNNPPIKSLTRCGQGQFECNDGICIADYKHCNGIVDCHDESDETGCNSDPCSSNEFSCDGQCYEMQAYCNGVPDCSDGSDERNCAICYGDAFHCKNKQCILSVHICDGEPHCSDGSDELECNNNTNCKVFEWQCRNGFCINKDFRCDGSVDCTDRSDEEGCYDEERPSDPCREDEFYCDGRCFEEDRRCDGRADCTDGSDEHDCPTLPLERCEELQCPDGSCFRHSQRCDGVSDCADGYDEQNCPCRSNEFTCADGQCIPNYLLCNGRPDCADGSDERNCSCARNEFRCRTGQCIDELRRCDQRIDCVDGSDEKDCTRICRSNEWTCHSGQCIRLDQRCNRRYDCQDRSDEMSCTSCGPNKFRCENGPCIAMALKCNGRVDCPYDTSDELDCTDEFQYAPPPPFDQPRLNLKTYPDEQTIKESNEVVFQCRDEGPMHAKVKWVRGNGQPLPPGTRDMNGRLEIPNIRIDHNGEYICEAVGYPKSTPGSSKVVQLTVERFHYHQRPPTACGVNEATCMNGNCILKSQICDGRQDCADNSDENGCSHKDLCEPNQFKCRNRKCVLKTWLCDGEQDCGDGSDEENCATLPPNAPCRHDEFQCRDGQCIPKSFQCDTHPDCQDKSDEVGCMAPAVIQPPPPSLSIIAGGVLNITCRATGVPVPLIVWRLNWGHVPEKCTSRNDQGFGRLTCEDMQPIDSGAYSCEIINTMGTHFVSPDTIVIVTGSGPVCQQGTFNSQARNPSDCINCFCFGVTTSCNSADLYTYALKPPVSSLTIVGVEGPWSGRRELVVGEFENHNLTAQRHGVQLRLTNLVPGRRVPYYSLPEEYKGNQLKSYGGSIRYDVEYDGTGRPVNAPDVILKGNGMSLVHWHSGTFYPDIKNHVSVSLLPGNWVRPDRSPATREDIMMALASIESILIRMQYVDGIERNIELVNVMMDSAAHDDRGLGSASLVEQCRCPAGYRGLSCESCDYGYVRQNSGPWLGRCVKRERECQPGYYGDPNRDIPCAPCPCPVAGEKSRARSCYLDSRDNVVCQCDRGYTGERCMECEEGYVGNPLGEGCFPRPVTNCNALGTERIGSDRRCYCKPGIEGTYCDRCAAQHFYMHEKGCVECFCMGLTNVCSSTTWVRDTIHASFADGRSGFSLISDYTNPSIVANALPVSNREIVYRNFGASDDTFYWRLPNQFLGNKLTSFGGHLNYTLRYTPHSSGGISRNSSPDVVLHSGNKIKLHHYRTNSPISPYGSNTHSVSIVEDEWQNYEDGNAATREYLLMALANVSDIFIKATYNTVSNEAALSYVSLDIARDSPYATGARAWPVEQCQCPTGHIGLSCEDCAPGYYKGDQGIYLGICEPCACNGHSDECDPQTGACINCRDNTYGNNCELCRPPYVGNATVGSRYDCTLSQPDQSFDCRECDVRGFTGNCQRGCECKQLVEGRRCDQCREGSFGLGTQHETGCVECFCSGVTKSCGSSNLYREELPVIVDAYDNTITLTDREGTLLESRNFAINPSINEISYTFRDRETYYWSLPNLVLGNQVLSYGANLTVTQYVEGGRPLADQDIILIGSGMKLAWSREYYDDGTYAVPLVEHNWIVINRRNSYPASRGDMLTVLSNLEHILIRATTKESTSVSRLSDITLGTAIPTRTPYPAVEVEMCRCPPGYRGTSCEQCEDLHYRDIYDRSAGLLGACKPCPCNYENADSCQMDNRGVVVCNCREGYSGDNCDNQLDTRPTVPPRNPVIEVVINVPSIKIVEIGEDFRADCTAHHVVSQSPIDVQWTRKNGRLPDSAYTDGGTLVITNIQIKDGGLYVCKASSGTEVVYKQVTINVSKQGSAKPTVLLSQPFIDQDEHLSAEVLCTATGYPTPTITWERVDQPLPYHAVTERGLLRFSALRQQDAGTYRCVARNDVGESDTLLTVYVRPAVGPTLPPSYPTEMERVEISPSSFDGQPGEVIRLICRCSPTAQVVWSKLGERQLPSNADVRNEMLIIEHASQENTGRYSCTAYFPNGRTKTSTVDVVIADNNVLPPSNKVAPRVNPLNKNYVVLQGADFSLSCEATGTPYPTVKWTLSGKTFESNVQQSGNVLRIFNAQPSNGGVYICVASNEEGMDRAYTVVEIDRREKPVLEIYPSEPQTIKVGESVRLSCRASAGVPYPSITWVRKDRMPLSSRFTNDAEGVITLRDASLEDAGEYECRADNAAGSAILSTTIEVLQPPIIRLQPSESHKITENDEFTIHCSATGKPAPMVTLLPPPGASRSHLRQQTEGLREVTLYLHQAELNDAGTYECTAKNAAGEDSQYLSLQVDPKRGDVGPHDDDDRSPSYYPPRLPPTRPPGPSSQPIAYTYKAILGEQAHLMCNEEFRSTRTEWRRSDGRPLPYGSIVRGGNLTIESTGHDAAGMYDCVTFPSPSQPVTIVRIMVEVISLPKISFSPQMPMTVRPGDAVDILCNVTSDVAYEVKWHRKDNLPLPPTVQAYGNYLRFSSIAPEDAGRYYCHVRNQFGETEKAAEVVVNKNELMPEPPIVGKRYHQASLGAAIRLDCKLPAGAPYVNVRYNWNRLERTLPPTARIVANGRELHLFNLEPEDAGTYQCGMSYPDGTVVYDSIVLTVAPESLPVLQLEPSRSMVRPGESLVVDCSSSAGTGVPIKWEKSDGTPLPYNIHQEGNRLYIQNAREDDSGTYTCVCFTEDGLRYVSDFQLEVEENTISDVLPRSTSRMEFAERGTTVKLQCNTDLYPTTYQWSRIDGELPNDRDTHGSILTLTNVQASDAGKYVCSAKHGGQSANVVITLVVNNVIPFFPQSPRSYMEFKSFDNVYSKFYFEVSFKPEKMNGLILYASQRRPNQDYISLSLRNGYPQFRFNFDGQQVVLQPEKPVHMGQWHTVKVNRVRNNGFLLVDDQTPVNFPDRLKFYGLNLDDHLFVGGVPQFDQVPASAVEYKEGFVGCISRLKLNDREVQLYEDALETVGITTCEPCADDPCKNFGTCLEAQTAQGYSCMCRDGYTGTNCQHEGDGCSDDTCGVGRCEETDTGPECYCPVHKTGDRCQYTEHYTDATLAFKDGSYAAYDKFQSKRSIRFRFKPDSLENGIMFYAAEHEQGYGDFMAVLLNNGFVEIRYSVAGKMKPLIVRSTVPIEAGQWHTVSAGRTKAGIGYLQVDDEAVNNEMSNRNTPILLKTKVYAGGYDKRLLLSQEIGVRRGFEGCIAELETSGNKLNMIDDIRDSANVYHCGHADGHHPDPIDVESPVTCRPGRGGYDCDTVTDICLDQRPCENGALCQTHAGGQNYTCTCEPGYLGQRCETQYTDIVASRFSGNGFIEVSPKAFKHSESQITTEFAIMFSAYEPNGLLIWYGQRNGEEFLGNDYVALSLSNGYVELTIRMDGQESYVRNSDVYVVDNERHVALVRRERNQFHLQVDSLTVHGETRPTGKQTMEIPGSFYVGGVPDVERVTGNRFNESFNGCVFSVENNEGKAILLRDFTIRTVNVDVCDEPNLGTDPPVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VGV8</accession>
    <name>A0A6E8VGV8_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Complexin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Positively regulates a late step in synaptic vesicle exocytosis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the complexin/synaphin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VGV8"/>
    <dbReference type="EnsemblMetazoa" id="ACON003509-RB">
        <property type="protein sequence ID" value="ACON003509-PB"/>
        <property type="gene ID" value="ACON003509"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_014259"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003509"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034498"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043195">
        <property type="term" value="C:terminal bouton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046928">
        <property type="term" value="P:regulation of neurotransmitter secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016079">
        <property type="term" value="P:synaptic vesicle exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.580:FF:000002">
        <property type="entry name" value="Complexin, isoform AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.580">
        <property type="entry name" value="Single Helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008849">
        <property type="entry name" value="Synaphin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16705">
        <property type="entry name" value="COMPLEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16705:SF4">
        <property type="entry name" value="COMPLEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05835">
        <property type="entry name" value="Synaphin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="4" id="KW-0532">Neurotransmitter transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="17"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003509-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="128" mass="15132" checksum="0F64FE914C20F262" modified="2020-08-12" version="1">MNFLGAVGGDGGDDENDKEKEEEAERERQEAIREAEERRKEKHRKMEEEREKMRQEIRDKYNIKKKEEIVEQAPQEEPNPLMRKKKTPEELAAEAEQEDLDDFTKLKNSIETQVNEIKTQIEGKCSLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VGY2</accession>
    <name>A0A6E8VGY2_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">F-box domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_040223614.1">
        <property type="nucleotide sequence ID" value="XM_040367680.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VGY2"/>
    <dbReference type="EnsemblMetazoa" id="ACON003661-RA">
        <property type="protein sequence ID" value="ACON003661-PA"/>
        <property type="gene ID" value="ACON003661"/>
    </dbReference>
    <dbReference type="GeneID" id="120949993"/>
    <dbReference type="CTD" id="43475"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010856"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003661"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038085"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031398">
        <property type="term" value="P:positive regulation of protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000556">
        <property type="entry name" value="CLUMA_CG018969, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000432">
        <property type="entry name" value="Uncharacterized protein, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20933:SF4">
        <property type="entry name" value="F-BOX INVOLVED IN POLYQ PATHOGENESIS, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20933">
        <property type="entry name" value="F-BOX ONLY PROTEIN 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="24"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003661-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="508" mass="57868" checksum="143A31DAF731A3DC" modified="2020-08-12" version="1">MDRELATTLMHRASLACTSNIFNQTSIEKLPDKVILHIFSYLSHLEICRMATVCRRWRTIAYNTQLWKNVSLRPEVSGLHVGSLESLLQLISVRFGPSLRYIELPIELITHTVLHELSSKCPNLTHMLLDFSTAMQLHDFSEMQAFPAKLRYMCICLSEVIFMEGFMRKIYNFINGLEVLHLIGTYEKCEEEEEEIYEVINVHKLKSATPNLRVINLYGINFIDDSHIDAFSSNCIQLECLAVNYCNKVTGSTLKALLARSKQLKCLLMNGTSLKSEYVMQAEWDKCSVQELDISGTDLSTECLIDLLTRIPTLRFLSAGQINGFNDSVLKAWMEGGNPKSLIALDLDSSDNISDEALARFITRYGAQLQACVLSGMTHITDQLWMSIMPILTNIRIIVMGTTERLGGNIHVDQLMDTIGSHCTKLERLELRWDPENLRFSDKSQKAIDLLRVKCLKLRCLVLSDGRYYETVKANFERADRTTVVRTTTSCRVSAYHVLSNYNDLVFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VH70</accession>
    <name>A0A6E8VH70_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glucose-methanol-choline oxidoreductase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GMC oxidoreductase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040227813.2">
        <property type="nucleotide sequence ID" value="XM_040371879.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VH70"/>
    <dbReference type="EnsemblMetazoa" id="ACON003785-RB">
        <property type="protein sequence ID" value="ACON003785-PB"/>
        <property type="gene ID" value="ACON003785"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON003785-RC">
        <property type="protein sequence ID" value="ACON003785-PC"/>
        <property type="gene ID" value="ACON003785"/>
    </dbReference>
    <dbReference type="GeneID" id="120952513"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004496"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003785"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040345"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016614">
        <property type="term" value="F:oxidoreductase activity, acting on CH-OH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.560.10">
        <property type="entry name" value="Glucose Oxidase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012132">
        <property type="entry name" value="GMC_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000172">
        <property type="entry name" value="GMC_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007867">
        <property type="entry name" value="GMC_OxRtase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552:SF147">
        <property type="entry name" value="CHOLINE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552">
        <property type="entry name" value="GLUCOSE-METHANOL-CHOLINE GMC OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05199">
        <property type="entry name" value="GMC_oxred_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00732">
        <property type="entry name" value="GMC_oxred_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000137">
        <property type="entry name" value="Alcohol_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00624">
        <property type="entry name" value="GMC_OXRED_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucose-methanol-choline oxidoreductase N-terminal" evidence="6">
        <location>
            <begin position="316"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="278"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="591"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000137-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003785-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="630" mass="69281" checksum="A97A44EFABD12D17" modified="2020-08-12" version="1">MEALMGGQCAAQSVGPANQLFGLLVQTILAAQCAISPPDMWPKDYGPTALQRGLDEYDFVIVGAGSAGSVVANRLSENPDWKVLLLEAGGDPPIESEIPETFFTIQKTDADWENYVEPTPHASKGSKDGAFWPRGRTLGGCGAINAMLYVRGNSRDYDGWAELGNPNWGWSDVLPYFKKSEDNHDSELLRRDGGKYHAAGGYLKVGNFPVNHPLAEVMLQAFKDAGFESTADINGARQVGFGRAQGTIVNGTRCSPAKAFLVPVKDRPNLHIIKHAVVVTVERDPSTERFKYVNFMIDNKVLKVAHARKEVILAAGAINTPHILQLSGIGPKALLEKVNIPLVADLPVGENLQDHLFVPLLFKMHKSTAENYNIQQELAKNLFQYIMTRSGPMAGHGVTSVIGFINTLDASSPFADIEYHFFQFEKGSGKSVLFCDKVGYTQEISQSMLEAATEADVVMVIVVLLNPKSKGRVTLATEDFNEFNPPRIQSGYLEAKEDVEAVLRGIRYINKIVDTPTFREHEGELHRMKLSECDELVYDSDDYWECYARYTTLTLYHPVGTAKMGPDSDKEAVVDARLRVKGVEGLRVVDGSIMPNIVSGNTNAPIMMIGEKASDMIKEDWGEGPNHTEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VH73</accession>
    <name>A0A6E8VH73_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein-synthesizing GTPase</fullName>
            <ecNumber evidence="3">3.6.5.3</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.3" evidence="3"/>
    <dbReference type="RefSeq" id="XP_040218655.1">
        <property type="nucleotide sequence ID" value="XM_040362721.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VH73"/>
    <dbReference type="EnsemblMetazoa" id="ACON003597-RA">
        <property type="protein sequence ID" value="ACON003597-PA"/>
        <property type="gene ID" value="ACON003597"/>
    </dbReference>
    <dbReference type="GeneID" id="120947408"/>
    <dbReference type="KEGG" id="acoz:120947408"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005491"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003597"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_036699"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005850">
        <property type="term" value="C:eukaryotic translation initiation factor 2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01888">
        <property type="entry name" value="eIF2_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03688">
        <property type="entry name" value="eIF2_gamma_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15490">
        <property type="entry name" value="eIF2_gamma_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000009">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000011">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000065">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050543">
        <property type="entry name" value="eIF2G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015256">
        <property type="entry name" value="eIF2g_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044127">
        <property type="entry name" value="eIF2g_dom_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044128">
        <property type="entry name" value="eIF2g_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003077">
        <property type="entry name" value="PRK04000.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42854">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42854:SF3">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09173">
        <property type="entry name" value="eIF2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="10">
        <location>
            <begin position="42"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003597-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="474" mass="51637" checksum="2F6CA05EFBD4F425" modified="2020-08-12" version="1">MSSGDQQMSTATTGQPHLQQQDLSKLDITKLTPLSPEVISRQATINIGTIGHVAHGKSTVVKAISGVQTVRFKNELERNITIKLGYANAKIYKCDNPKCLRPTCFTSGGSSKDDSFPCYRPACTGRFQLVRHVSFVDCPGHDILMATMLNGAAVMDAALLLIAGNESCPQPQTSEHLAAIEIMKLKHIIILQNKIDLVKDSQAKEQYDQIVKFVQGTVAEGAPIIPISAQLKYNIEVLCEYITKKIPIPPRNFIDAPRLIVIRSFDVNKPGCEVNDLKGGVAGGSILRGVLKVGQEIEVRPGLVSKDAEGRLTCKPIFSKIVSLYTEQNELQFAVPGGLIGVGTKIEPTLCRADRLVGQVLGAVGALPNIFIELEVSYYLLKRLLGVRTEGDKKGAKVQKLVRHEMLLVNIGSLSTGGRVVATRADLAKIALTNPVCTEKNEKIALSRRVENHWRLIGWGQIRGGTVIEPLKEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VH74</accession>
    <name>A0A6E8VH74_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glucose-methanol-choline oxidoreductase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GMC oxidoreductase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040227814.2">
        <property type="nucleotide sequence ID" value="XM_040371880.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VH74"/>
    <dbReference type="EnsemblMetazoa" id="ACON003785-RA">
        <property type="protein sequence ID" value="ACON003785-PA"/>
        <property type="gene ID" value="ACON003785"/>
    </dbReference>
    <dbReference type="GeneID" id="120952513"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004496"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003785"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040345"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016614">
        <property type="term" value="F:oxidoreductase activity, acting on CH-OH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.560.10">
        <property type="entry name" value="Glucose Oxidase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012132">
        <property type="entry name" value="GMC_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000172">
        <property type="entry name" value="GMC_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007867">
        <property type="entry name" value="GMC_OxRtase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552:SF147">
        <property type="entry name" value="CHOLINE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552">
        <property type="entry name" value="GLUCOSE-METHANOL-CHOLINE GMC OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05199">
        <property type="entry name" value="GMC_oxred_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00732">
        <property type="entry name" value="GMC_oxred_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000137">
        <property type="entry name" value="Alcohol_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00624">
        <property type="entry name" value="GMC_OXRED_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucose-methanol-choline oxidoreductase N-terminal" evidence="6">
        <location>
            <begin position="313"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="277"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000137-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003785-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="629" mass="70003" checksum="9BB8A7141CC0BF85" modified="2020-08-12" version="1">MEALMGGQCAAQSVGPANQLFGLLVQTILAAQCAISPPDMWPKDYGPTALQRGLDEYDFVIVGAGSAGSVVANRLSENPDWKVLLLEAGGDPPIESEVPYLAFALLNGSHVWNYYAERSDTASKGYKRGSYWPRGKMLGGSSSNNIMLYVRGNSRDYDRWEEQGNPGWGWKDVLEYFKKSEDNGAQHLLQERADYHAQGGLLKVNSFMSNDMTKLVITEAAQELGIPEIMDINSDEYIGYNVAQGTVHKGRRWSTAKAFLNTAADRHNLHIIKNAHVTKINFEGTAATGVTFDVPSQTGVSASIRKEVIISAGAINTPQVLQLSGLGAKEQLDRLDIPLVKEIPSVGENLQDHLIVPLFLSLHGSRPIERSMDELLDSIYSYFRYGLGTFGTVGITDLLAFVNTQSPAAKFPDIQYHHSLILWKTPDIARLTQCFGWEDYISHQIIEQNQKSEILMVMVTLLNPKSKGNVQLRSSNPYDAPIINANYLDDQRDVKTIIRGIRFFRKLLDTENFGYHELKEFHLKIEECDRLEYESDSYWECYARYMSSTIYHPTGTAKMGPDGDQASVVDSRLKVRGVQNLRVIDASIMPDIVSGNTNAPTIMIGEKGADMIKEDYGVEKKEAATHTEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VH93</accession>
    <name>A0A6E8VH93_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Annexin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="domain">
        <text evidence="6">A pair of annexin repeats may form one binding site for calcium and phospholipid.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the annexin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_049463109.1">
        <property type="nucleotide sequence ID" value="XM_049607152.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VH93"/>
    <dbReference type="EnsemblMetazoa" id="ACON003790-RC">
        <property type="protein sequence ID" value="ACON003790-PC"/>
        <property type="gene ID" value="ACON003790"/>
    </dbReference>
    <dbReference type="GeneID" id="120947902"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004607"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003790"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_029479"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012506">
        <property type="term" value="C:vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001786">
        <property type="term" value="F:phosphatidylserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032509">
        <property type="term" value="P:endosome transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000001">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000002">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000004">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000010">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.10">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001464">
        <property type="entry name" value="Annexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018502">
        <property type="entry name" value="Annexin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018252">
        <property type="entry name" value="Annexin_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037104">
        <property type="entry name" value="Annexin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10502">
        <property type="entry name" value="ANNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10502:SF233">
        <property type="entry name" value="ANNEXIN B9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00191">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00196">
        <property type="entry name" value="ANNEXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00335">
        <property type="entry name" value="ANX"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47874">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00223">
        <property type="entry name" value="ANNEXIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51897">
        <property type="entry name" value="ANNEXIN_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0041">Annexin</keyword>
    <keyword evidence="3 6" id="KW-0106">Calcium</keyword>
    <keyword evidence="5 6" id="KW-0111">Calcium/phospholipid-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003790-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="324" mass="35705" checksum="852B41465ED31AA2" modified="2020-08-12" version="1">MSSSQYYPFQCTPTVYPADPFDANEDAATLRTAMKGFGTDEKAIIEVLARRGIVQRLEIAQAFKTSFGKDLISDLKSELGGKFEDVILALMTPLPQFYAKELHDAISGIGTDEEAIIEILCTLSNYGIRTIAEFYEQMYNVSLESDLKGDTSGAFKRLCVSLVQGNRDENNGVDEGAAAADAQALFEAGEGQWGTDESVFNQILVTRSYQQLRAVFDIYESLAGHSIEDAIKREFSGAIEEGFKAIVRCVRSKVQYFAKRLHNSMAGLGTNDKTLIRIIVSRSEIDLGDIKEAFQEMYGKSLESWIKEDVGGDLGDLLATLASF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VKR0</accession>
    <name>A0A6E8VKR0_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">K Homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VKR0"/>
    <dbReference type="EnsemblMetazoa" id="ACON004942-RA">
        <property type="protein sequence ID" value="ACON004942-PA"/>
        <property type="gene ID" value="ACON004942"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002542"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004942"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040741"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd22438">
        <property type="entry name" value="KH-I_PCBP_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02396">
        <property type="entry name" value="KH-I_PCBP_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22439">
        <property type="entry name" value="KH-I_PCBP_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000005">
        <property type="entry name" value="poly(RC)-binding protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10288">
        <property type="entry name" value="KH DOMAIN CONTAINING RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="3">
        <location>
            <begin position="2"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="3">
        <location>
            <begin position="73"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="3">
        <location>
            <begin position="243"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004942-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="341" mass="36155" checksum="F7F17190A60877FA" modified="2020-08-12" version="1">EVGSIIGKKGEIVKRFREESGAKINISDCSCPERIVTVSGSRSAIYKAFTLITKKFEEWCSQFQDNANTQGKPQIPIRLIVPASQCGSLIGKGGSKIKEIREITGCSIQVASEMLPNSTERAVTLSGSAEAITQCIYHICCVMLESPPKGATIPYRPKPQVNGPVIVANGQAYTIQGNYAVPAQEVSGIAKNPLAGLAALGLAGAIPSNTGGLNPTGKYSSPLAALAGSQLRTNNNRNVAPVQSQSHEMTVPNDLIGCIIGKGGTKIAEIRQISGAMIRISNCEERDSGNTDRTITITGNPDSVALAQYLINMRISMETAGMPIPGYHYITPNHIVKAPIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VKR7</accession>
    <name>A0A6E8VKR7_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcription elongation regulator 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VKR7"/>
    <dbReference type="EnsemblMetazoa" id="ACON004745-RB">
        <property type="protein sequence ID" value="ACON004745-PB"/>
        <property type="gene ID" value="ACON004745"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004585"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004745"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_036008"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070063">
        <property type="term" value="F:RNA polymerase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.440:FF:000006">
        <property type="entry name" value="Transcription elongation regulator 1 (CA150)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.440:FF:000001">
        <property type="entry name" value="Transcription elongation regulator 1 like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000049">
        <property type="entry name" value="Transcription elongation regulator 1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.440:FF:000029">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.440">
        <property type="entry name" value="FF domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002713">
        <property type="entry name" value="FF_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036517">
        <property type="entry name" value="FF_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045148">
        <property type="entry name" value="TCRG1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057565">
        <property type="entry name" value="WW_TCRG1_3rd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15377">
        <property type="entry name" value="TRANSCRIPTION ELONGATION REGULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15377:SF3">
        <property type="entry name" value="WW DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01846">
        <property type="entry name" value="FF"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23517">
        <property type="entry name" value="WW_TCERG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00441">
        <property type="entry name" value="FF"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81698">
        <property type="entry name" value="FF domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51676">
        <property type="entry name" value="FF"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="WW" evidence="3">
        <location>
            <begin position="79"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="3">
        <location>
            <begin position="238"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="3">
        <location>
            <begin position="406"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="domain" description="FF" evidence="4">
        <location>
            <begin position="566"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="domain" description="FF" evidence="4">
        <location>
            <begin position="633"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="domain" description="FF" evidence="4">
        <location>
            <begin position="701"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="domain" description="FF" evidence="4">
        <location>
            <begin position="885"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="367"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="458"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="719"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="32"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="367"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="465"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="719"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="791"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="805"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="822"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="839"/>
            <end position="863"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004745-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1027" mass="116455" checksum="7687A6CD6C1663AD" modified="2020-08-12" version="1">MSTEVDKPLPNEAESTEEVSNAGTIDGDSVVASASEQHTSPSAADSTLEENKVSPIKNGAGLLGSAPSKLANTVATDLWVETKTADGKSYYYHAISRETTWTRPEGPNVNVMTQAEVEALNKQQQQQQHTKSAEQKVIEVNASSASDIPVSSATIPAVIPLHTAMTRFTGPPPSFGMAPFGMPPPNFTSFPPWNPPAVGSGQNWSIGQTLPLDAAKQAMNEIKLNEIDPSIVARATVWSEHTAPDGRMFYYNASKGESVWEKPQALRDLEKAKLTVWNAKQKSKAPPSVPPFLAPPIPPQMPLMSAMQAPPATGLKIQQFPSPLATAAIGPVSGSGVRLQQLPVAASGVVFDPITFMAKNEKAAAEEERKRKLEQEKKRKEDEEKAKSNKPQDKSRPISSTPISGTPWCVVWTGDGRVFFYNPSTRTSVWERPEELKERADVDKAVLVPPQQLLGTVAPKELDGVTSSKGQTVLGTGNSATATLAESDGGQTASGGNTNEPRSAQSDSESSVDEDGEHPSKKLKSDLEAVMKSTHSLGSNRSGTDPEKEAIAMEAEARAARERTLIPLDVRMKSFREMLRELDVSAFSTWEKELHKIVYDARYLLLTSKERKQVFEKYVKDRADEERREKRNKMRQKRDDFRALMDAAHLHGKSSFSEFAQKYGKDERFKVIEKIRERESLFNEYIVEIRKREKEEKQIRKEQIRKDFLTMLRERSDINRHTRFSDIRKKVESDSRYKAITEHSQREELFEDHIKALKEEKRKAKEKEREKELKERNSRSEQRSSRKSRDRSRDRRSRSNDREASQSAKGASNHDGTANNNNDEDEGEDCNTSDEDEIERQQRERERKARAEASIKEREKEVQRTLATHLRDRDKELFNEHISNLVRKKRDKFREMLDEIPSLELTSSWKDIKKSIRDDPRYLKYNSSERGEREFRDYIKDKTANAKLAFRELLQECKFITHKSFELYRENANHLREVEDILRNDSRYLILHHIAGERTQMILAHLEELHKRGPPPPPTASESLRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VKR9</accession>
    <name>A0A6E8VKR9_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Endoplasmic reticulum vesicle transporter C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
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            <dbReference type="DOI" id="10.3390/genes10010062"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain>Mali-NIH</strain>
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    <sequence length="287" mass="32666" checksum="8DFB5302A032934B" modified="2020-08-12" version="1">MPCKSIGADILDSTNQNVFSFGILQEEDTWFELCPSQRVHFDYMQHHNSYLRNEYHSIAEILYKSDHAVVYSMPERVIIPEKPHDACRIHGVLTLNKVAGNFHITVGKTIHFSRGHIHLNSIFANTQTNFSHRINRFSFGDHTAGIIHPLEGDEKLFDNGQVMMQYFIEVVPTDVQKFYSHSKTYQYTVRENLQLIDIDKGMQGVAGIYFKYDMSALRVLVRQDRDSIAHFIVRLSSIIAGIVVISGMLSKCMHLIGDACCKRRYNDEPIARNNDGTATVKGAVIVS</sequence>
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                <person name="Lambert C.C."/>
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                <person name="Galvin B.D."/>
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        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
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                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
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                <consortium name="EnsemblMetazoa"/>
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    </evidence>
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    <sequence length="293" mass="34076" checksum="6438F82FCDDC32B1" modified="2020-08-12" version="1">GKKIIHLLKVLLNYLFYTNMVKETVLEKANNCAQEYSELNAKLNQEQITKEEKKIRANKINRHIDDLKLHLPQLKNQIETLQQRKHKLQENISTLKANDQQLADKIIKLKIEHSELAELLVADDEASSVREIKQSLTREIETLTETEKELQQAYQIHVSSINQIRPCNALLEKMLLIGMDESCKNLRGAIVELNSLCDKLRKQRNNLTNLSDTLQNNCEELDGLLADKNQELEVRRKVLEKNDRRKDSKHLEQEKRLKALDQANEIYAQLLVVQKAEMQKVIVMVEQALKFIN</sequence>
</entry>
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        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
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                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
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    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
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        <subcellularLocation>
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            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the endophilin family.</text>
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        <location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="5">
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    <feature type="compositionally biased region" description="Polar residues" evidence="5">
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004766-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="297" mass="33243" checksum="58D0488AE6381CD9" modified="2020-08-12" version="1">MAFAGLKKQINKANQYVTEKMGGAEGTKLDLDFMEMERKTDVTVELVEELQAKTKEYLQPNPTARAKMAAVKEGILADCMLTYGKKLGEDSIFASALVEMGDSLKQMADVKYSLDDNIKQNFLEPLHQLQTKDLKEVMHHRKKLQGRRLDFDCKKRRQAKGSQITDDEIRSAEEKFAESLQLAQVGMYNLLENDVEQVSQLVTFAEGMLDYHSQCSEILKVLVETLNEKREEAAARPKSEFVPKTLADLNIEMLGSQDGMNGGSSSHLSSPVNKPTHLELLGNGQAQARSTNGKRNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VL31</accession>
    <name>A0A6E8VL31_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Scavenger receptor class B member 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">SR-BI</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Plays an olfactory role that is not restricted to pheromone sensitivity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <location evidence="2">Caveola</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CD36 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VL31"/>
    <dbReference type="EnsemblMetazoa" id="ACON004846-RC">
        <property type="protein sequence ID" value="ACON004846-PC"/>
        <property type="gene ID" value="ACON004846"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006380"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004846"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042930"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005901">
        <property type="term" value="C:caveola"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005044">
        <property type="term" value="F:scavenger receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002159">
        <property type="entry name" value="CD36_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11923:SF110">
        <property type="entry name" value="SCAVENGER RECEPTOR CLASS B MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11923">
        <property type="entry name" value="SCAVENGER RECEPTOR CLASS B TYPE-1 SR-B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01130">
        <property type="entry name" value="CD36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01609">
        <property type="entry name" value="CD36FAMILY"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="450"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00040821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00042244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004846-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="512" mass="57457" checksum="8F5E6C8EAB080CAF" modified="2020-08-12" version="1">MFLGRRNFKLWFVIAFALSLLVLGALVTFGFTAFIRTIIDHQVALRVGGQSFGWWSRPPVEPIIRIFVYNVTNADEFLNNGTKPILDELGPYVYVQTWEKVNIKENPNGTISYNQKRVYIFNEDLSGGLEDDVVIVPNIPMLSATSQSKHAARFLRLAMASIMDILKIKPFVEVSVGQLLWGYEDPLLKLAKDVVPKEQKLPYEEFGLMYGKNSTSKDTVTVWTGVDDINQYGIIDKYNGRSHQTHWLSEQCNRLNGTDGSIFPPRITKNSTLHVYEKDLCRLLPLSFEKEVTVRGGVKGYRFTPSPDVFASVDKNPNNMCYCPAGPPCAPHGLFNVSLCQYDSPILLSFPHFYMADQTLRTAVEGISPPEKDKHQLFIDVQPDMGTALRARARIQINLAVSQVVDIKQVANFPDIVFPILWFEEGIDSLPDEILDLMKVATNIPPRAKFILTIALFGLGGFLFVVAVICLVRKSHRQSTLHLEGSNYLATASVDQAKKKAKMDNGMSSKSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VL33</accession>
    <name>A0A6E8VL33_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Macro domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VL33"/>
    <dbReference type="EnsemblMetazoa" id="ACON005091-RA">
        <property type="protein sequence ID" value="ACON005091-PA"/>
        <property type="gene ID" value="ACON005091"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON005091-RB">
        <property type="protein sequence ID" value="ACON005091-PB"/>
        <property type="gene ID" value="ACON005091"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_013305"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005091"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032702"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48411">
        <property type="entry name" value="OS01G0948300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48411:SF1">
        <property type="entry name" value="OS01G0948300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13716">
        <property type="entry name" value="CRAL_TRIO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="1">
        <location>
            <begin position="45"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005091-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="218" mass="25096" checksum="F85C05D7322FD6FD" modified="2020-08-12" version="1">MAQMVTMESIDLSDATISIPPEIQVDQIHIETPEERYERLLRRAKTEDLSEVSGIGCLYQSGVDRLGRPVVVFCGKWFPAHNIDLEKALLYLIYLLDPIVKGDYVIAYFHTLTSSNNYPSLQWLKDVYSILPYKYKKNLKAFYIVHPTFWTKMMTWWFTTFMAPAIKTKVHSLPGVEHLYSAIAKDQLEIPAYITEYDMATNGIHYFQPITNTSSPAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VL63</accession>
    <name>A0A6E8VL63_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Guanine nucleotide-binding protein subunit alpha</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the G-alpha family. G(q) subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VL63"/>
    <dbReference type="EnsemblMetazoa" id="ACON005079-RK">
        <property type="protein sequence ID" value="ACON005079-PK"/>
        <property type="gene ID" value="ACON005079"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007505"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005079"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032935"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005834">
        <property type="term" value="C:heterotrimeric G-protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001664">
        <property type="term" value="F:G protein-coupled receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031683">
        <property type="term" value="F:G-protein beta/gamma-subunit complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007188">
        <property type="term" value="P:adenylate cyclase-modulating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050793">
        <property type="term" value="P:regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00066">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.400.10:FF:000002">
        <property type="entry name" value="guanine nucleotide-binding protein G(Q) subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003977">
        <property type="entry name" value="Guanine nucleotide-binding protein G(q) subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000692">
        <property type="entry name" value="Guanine nucleotide-binding protein subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.400.10">
        <property type="entry name" value="GI Alpha 1, domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000654">
        <property type="entry name" value="Gprotein_alpha_Q"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001019">
        <property type="entry name" value="Gprotein_alpha_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011025">
        <property type="entry name" value="GproteinA_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218">
        <property type="entry name" value="GTP-BINDING PROTEIN ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218:SF329">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN G(Q) SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00503">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00318">
        <property type="entry name" value="GPROTEINA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00442">
        <property type="entry name" value="GPROTEINAQ"/>
    </dbReference>
    <dbReference type="SMART" id="SM00275">
        <property type="entry name" value="G_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47895">
        <property type="entry name" value="Transducin (alpha subunit), insertion domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51882">
        <property type="entry name" value="G_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9 11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4 10 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 9 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0564">Palmitate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0807">Transducer</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="43"/>
            <end position="48"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="149"/>
            <end position="150"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="174"/>
            <end position="180"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="199"/>
            <end position="203"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="268"/>
            <end position="271"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="325"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU369122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005079-PK"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="353" mass="41645" checksum="00DE40A054AEF77E" modified="2020-08-12" version="1">MECCLSEEAKEQKRINQEIERQLRRDKRDARRELKLLLLGTGESGKSTFIKQMRIIHGSGYSDEDKRGFIKLVYQNIFMAMQSMIRAMDLLKILYSDPANIEHAELIRSVDFETVTTFEPPYVQAIKDLWADAGIQECYDRRREYQLTDSAKYYLSDLARIEAEDFLPTEQDILRARAPTTGILEYPFDLDSIIFRMVDVGGQRSERRKWIHCFENVTSIIFLVALSEYDQILFESENENRMEESKALFKTIITYPWFQHSSVILFLNKKDLLEEKIMYSHLVDYFPEYDGPKHDDVKAREFILKVYLRENPDPDRMCYSHFTCATDTENIRFVFAAVKDTILQSNLKEYNLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VL69</accession>
    <name>A0A6E8VL69_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Aldedh domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VL69"/>
    <dbReference type="EnsemblMetazoa" id="ACON005124-RC">
        <property type="protein sequence ID" value="ACON005124-PC"/>
        <property type="gene ID" value="ACON005124"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006213"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005124"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031069"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004029">
        <property type="term" value="F:aldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006081">
        <property type="term" value="P:aldehyde metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07132">
        <property type="entry name" value="ALDH_F3AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000003">
        <property type="entry name" value="Aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000004">
        <property type="entry name" value="Aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012394">
        <property type="entry name" value="Aldehyde_DH_NAD(P)"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43570">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43570:SF16">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE TYPE III, ISOFORM Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="2 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="584"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="8">
        <location>
            <begin position="120"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="25"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="50"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="61"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="71"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005124-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="609" mass="67150" checksum="AAA9E8D6D74B40B5" modified="2020-08-12" version="1">MDKPKSALNIAEETRLFGEVPQVTLALSGTETSKPPSVKENVLKPSSDSIENHDGQHDTKSSGISSTTSSMKDDPSRDGQHLDESNLGQQPDSMTTVVIDIEATSGGSIVPDYINMSFSDIVQQLRTTFNSGKTRDVDFRLNQLKALLRCYEENTAEMANVLAADLRKHKQEAHLLEIEFILNDLRNTLYNLREWVKPEKPEKSLVNVMDGVYIYKDPYGVVLVIGAWNYPLQLTLVPVAAAIAAGNCVVIKPSEVAPASAKFIAETLPKYLSQDCYRVVTGGPKETSELLKEKFDYVFYTGSGRVGKIIHQACSENLTPCTLELGGKSPCYLDGTANIAIAARRILWGKFINAGQTCIAPDYILCSKQVQKQFLEEARKVLNEWYGANPKDSPDLCRIINQNHFQRLTSLLKGANVAIGGETDLQDRYISPTILIDVKSTDPVMQDEIFGPILPILNVDNAYDAIKFINSRDKPLCLYLFTENIEDRDAFIANTSSGGVCVNDALTHFAVEALPFGGVGPSGMGSYHGKYSFDTFVHKKSCLTKDFNPIGEKLAASRYPPYSESKLSFLSTLLKKRQGINLHFLPYLLMFGIGVASTLVVSTILKDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VLA7</accession>
    <name>A0A6E8VLA7_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">ARID domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VLA7"/>
    <dbReference type="EnsemblMetazoa" id="ACON005156-RB">
        <property type="protein sequence ID" value="ACON005156-PB"/>
        <property type="gene ID" value="ACON005156"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006480"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005156"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042303"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
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    </dbReference>
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    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="ARID" evidence="8">
        <location>
            <begin position="17"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="REKLES" evidence="9">
        <location>
            <begin position="479"/>
            <end position="580"/>
        </location>
    </feature>
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        <location>
            <begin position="123"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="228"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
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            <begin position="513"/>
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    </feature>
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            <begin position="584"/>
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        <location>
            <begin position="666"/>
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    </feature>
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            <begin position="389"/>
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        <location>
            <begin position="132"/>
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        </location>
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        <location>
            <begin position="236"/>
            <end position="246"/>
        </location>
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        <location>
            <begin position="267"/>
            <end position="279"/>
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        <location>
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        </location>
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            <end position="313"/>
        </location>
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        <location>
            <begin position="341"/>
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        </location>
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        <location>
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            <end position="469"/>
        </location>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="693" mass="76448" checksum="70EDE15110A36427" modified="2020-08-12" version="1">MLPVDNQLSELYEINDDPKRKEFLDDLFLFMQKRGTPINRLPIMAKSVLDLYELYNLVIARGGLVDVINKKLWQEIIKGLHLPSSITSAAFTLRTQYMKYLYPYECEKKNLSTPAELQAAIDGNRREGRRSSYGQFDSPSVQRSPIPQGISQMPPLSLVSHVNQQQHHHRLLPPVGQIPNMLPAEFEQRMLNYIKMFQPKEMGRSQSPDKLSALNAIDISRVALWNMYNPSPNSPPNSLNTSPENNMQSALDLSESPDHAMAAIKRERDHPVDFSDPRDYGNGLGPPAKRSHHHHHSHHHNRSLTPQKLNSSSQDEDDARSDSTPVNLRENGSHGRRATLDDDGDDEEDEMEDETTDEDMLRASFAAAAAAAAVNQHPLALSLADHHLQQQQQQQQQQQQRERERIQQQVAAQQQQQQQQQQREQQHSLATLDLSILDRERLRCDSADKLKRQSQQQQQQQQQQQQQHLLDQHHLHQRQLQHQQQARVARELRKHENGDRLSLTPDSSELLHHSKNHLSTSGGGGGAGSDPFSSPLGITGMQFKLISRGDASKGDQKLVVTMEFNGVQYEGVLFANPLSSANLSAPPASTPPSSSVSATSASPIGSMVSPVKSSAAAHIASPLDERDSSASANIDVAAMGSALAATATVTSNASSNATCIQQQPLDGAVSPPAALSITAPSVGASSPARSLVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VLC0</accession>
    <name>A0A6E8VLC0_ANOCL</name>
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        <recommendedName>
            <fullName evidence="4">EB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_040228806.1">
        <property type="nucleotide sequence ID" value="XM_040372872.2"/>
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    <dbReference type="EnsemblMetazoa" id="ACON004943-RC">
        <property type="protein sequence ID" value="ACON004943-PC"/>
        <property type="gene ID" value="ACON004943"/>
    </dbReference>
    <dbReference type="GeneID" id="120953151"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001776"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004943"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033612"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="entry name" value="EB_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01683">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="230"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="EB" evidence="4">
        <location>
            <begin position="161"/>
            <end position="205"/>
        </location>
    </feature>
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            <begin position="283"/>
            <end position="341"/>
        </location>
    </feature>
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            <begin position="413"/>
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            <begin position="354"/>
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            <begin position="285"/>
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            <begin position="422"/>
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    </feature>
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        </source>
    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="451" mass="50108" checksum="D94F9ADCE56DE22B" modified="2020-08-12" version="1">MIVSFFPDSVWGCTVKQHQKQKHLLYHKIIYTLISDKYKNRILRKSSFALLYLSYVFIKMLPVTLAIETHTGTKSITSLAAKTRPLPEMVAPPLDDEDDDYFDDYVDEYDIVNNKSAKGMYVGAPCEFTCHAKLHHVYCDPTTNTCSCEKDYVVLIGILKGCAKPKKLGEQCFYHQTCIYNDEHAMCVQIVHNAICQCAPGFHSVSYSKPTKRVFCTQDMATITADLPTLFGVTSGIAVLAGLICMVLHLFSKTKYPRPRHFGDANLAPPILFSSDTGIPLTIQSARPSSRSSQRSSGSIGSYGNRRSSSAPLHGSKGVLVSTSRTGSRRPSLASVHSTSSSVRSYSMRRFEQEIQQKELRQEMKRHLARLQEQQQLQKIKPNIIIGDVISCPMNKPILTTPSPMTPNSVDDILPSVDETQEFSPNLSQRSVSDSTGGCFDGPCSSTELLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VLC3</accession>
    <name>A0A6E8VLC3_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Peptidoglycan recognition protein family domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VLC3"/>
    <dbReference type="EnsemblMetazoa" id="ACON005203-RA">
        <property type="protein sequence ID" value="ACON005203-PA"/>
        <property type="gene ID" value="ACON005203"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_014474"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005203"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033832"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008745">
        <property type="term" value="F:N-acetylmuramoyl-L-alanine amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009253">
        <property type="term" value="P:peptidoglycan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06583">
        <property type="entry name" value="PGRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.80.10:FF:000001">
        <property type="entry name" value="Peptidoglycan recognition protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.80.10">
        <property type="entry name" value="Peptidoglycan recognition protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036505">
        <property type="entry name" value="Amidase/PGRP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002502">
        <property type="entry name" value="Amidase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015510">
        <property type="entry name" value="PGRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006619">
        <property type="entry name" value="PGRP_domain_met/bac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11022">
        <property type="entry name" value="PEPTIDOGLYCAN RECOGNITION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11022:SF41">
        <property type="entry name" value="PEPTIDOGLYCAN-RECOGNITION PROTEIN LC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01510">
        <property type="entry name" value="Amidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00644">
        <property type="entry name" value="Ami_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00701">
        <property type="entry name" value="PGRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55846">
        <property type="entry name" value="N-acetylmuramoyl-L-alanine amidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0391">Immunity</keyword>
    <keyword evidence="2" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="218"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidoglycan recognition protein family" evidence="7">
        <location>
            <begin position="255"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetylmuramoyl-L-alanine amidase" evidence="6">
        <location>
            <begin position="267"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="171"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="8"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="50"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="171"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005203-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="439" mass="47445" checksum="A1D37CEF7AEAC50C" modified="2020-08-12" version="1">MALARNLQLENDTSVNTGYHTKAQSMSASGGDTIAGLNNSGTGTGKSDSAKGSSLSNISASSIKRTDNGGDDDDSSVFDSSSSDTECDDDSIKRAIDRIPGTLAPGEARVLPNANVKVAVEAIAPGVRPSPAASTIGAIAVHNSSDITFGNKTYIKGQVVIKNIYQDRKNGTTNQGYQVQENETDDTNSTKKVPSATKPPEPRTWQSSLKTIIKDKPLLSFIVMVSLMIVLCAIVAVISILTASEDDLFPDPRPLRLVTRTEWLAQPPREELTDLKLPVNNVIIAHTATEGCTTQTKCMYQVKLIQEFHSSPDSRNFSDIAYQFLVGGDGNAYEGRGWTKQGAHTKGFNVDSICIAFIGTFIADPPPIAQLSAAKQLILLGMKENYLASNYSLYGHRQLAPFESPGKALFDIIKTWPHWSNKLGSNHWVEPANDTTNRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VMV5</accession>
    <name>A0A6E8VMV5_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Ig-like domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VMV5"/>
    <dbReference type="EnsemblMetazoa" id="ACON005599-RA">
        <property type="protein sequence ID" value="ACON005599-PA"/>
        <property type="gene ID" value="ACON005599"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001584"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005599"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039931"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005230">
        <property type="term" value="F:extracellular ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099095">
        <property type="term" value="F:ligand-gated monoatomic anion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18987">
        <property type="entry name" value="LGIC_ECD_anion"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19049">
        <property type="entry name" value="LGIC_TM_anion"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000021">
        <property type="entry name" value="glutamate-gated chloride channel isoform X12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006028">
        <property type="entry name" value="GABAA/Glycine_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00253">
        <property type="entry name" value="GABAARECEPTR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="503"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="567"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="686"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="13">
        <location>
            <begin position="295"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="14">
        <location>
            <begin position="510"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="151"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="22"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="39"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="70"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="165"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="185"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005599-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="711" mass="79302" checksum="2CC1AED582F3565C" modified="2023-06-28" version="2">MGYSCESAHLQYRPVRSDRNQRSWSTEPLQLLDSHQPQATTATDRSCTSDSTTTTITNHSPAPNQPPWATTTDSSSTSPTRWNLRTEPTPEGTVAAPVTKRRTSPTEPIAVTSSRWHSSFNLLRAPTAVFDGYSTIVLEKLSRFISRHVAAPQHATPPSVPAIPTTRATTKTTSRSAARPMAHTVTPSSRPAHGSSTSSSTVRRHDDEPCRSDGQPVPLGHRAHRQPTQFHAPSVQRDVAASPTASRPASSHHLVRHSLCLYAASSVLLALCYLTIPTVASDTAHPIFAEGKSDKEILDHLLKNSRYDKRLLPPVDGTLTVNVSVLLLSLASPDESSLKYEVEFLLQQQWFDPRLRYANQSQYEYLNAIHHHEDIWLPDTYFIMHGDFKDPIIPMHFALRIYRNGTINYLMRRHLILSCQGRLNIFPFDDPLCSFALESISYEQSAITYVWKNDEDTLRKSPSLTTLNAYLIQNQTIACPIKASWRGNYSCLKVDLMFTRDRAFYFTTVFIPGIILVTSSFITFWLEWNAVPARVMIGVTTMLNFFTTSNGFRSTLPVVSNLTAMNVWDGVCMCFIYASLLEFVCVNYVGRKRPLHNVVYRPGENPVTQRLPAVLSRIGIILASPLGVKKRESGGPNEIVACTSCAGGTSPCTHSANNGCATETCFVQVRKKEPPHPIRVAKTIDVIARITFPSAYAVFLIFFFIHYKGFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VMV9</accession>
    <name>A0A6E8VMV9_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">phenylalanine 4-monooxygenase</fullName>
            <ecNumber evidence="5">1.14.16.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Phe-4-monooxygenase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(6R)-L-erythro-5,6,7,8-tetrahydrobiopterin + L-phenylalanine + O2 = (4aS,6R)-4a-hydroxy-L-erythro-5,6,7,8-tetrahydrobiopterin + L-tyrosine</text>
            <dbReference type="Rhea" id="RHEA:20273"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15642"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
            <dbReference type="ChEBI" id="CHEBI:59560"/>
            <dbReference type="EC" id="1.14.16.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 13">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 1/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.</text>
    </comment>
    <dbReference type="EC" id="1.14.16.1" evidence="5"/>
    <dbReference type="RefSeq" id="XP_040222659.1">
        <property type="nucleotide sequence ID" value="XM_040366725.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VMV9"/>
    <dbReference type="EnsemblMetazoa" id="ACON005712-RB">
        <property type="protein sequence ID" value="ACON005712-PB"/>
        <property type="gene ID" value="ACON005712"/>
    </dbReference>
    <dbReference type="GeneID" id="120949426"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010383"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005712"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_036069"/>
    <dbReference type="UniPathway" id="UPA00139">
        <property type="reaction ID" value="UER00337"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004505">
        <property type="term" value="F:phenylalanine 4-monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048066">
        <property type="term" value="P:developmental pigmentation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042416">
        <property type="term" value="P:dopamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006559">
        <property type="term" value="P:L-phenylalanine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd04904">
        <property type="entry name" value="ACT_AAAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03347">
        <property type="entry name" value="eu_PheOH"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Tyrosine 3-monooxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.800.10">
        <property type="entry name" value="Aromatic amino acid hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001273">
        <property type="entry name" value="ArAA_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036951">
        <property type="entry name" value="ArAA_hydroxylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036329">
        <property type="entry name" value="Aro-AA_hydroxylase_C_sf"/>
    </dbReference>
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        <property type="entry name" value="Aromatic-AA_hydroxylase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041912">
        <property type="entry name" value="Euk_PheOH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005961">
        <property type="entry name" value="Phe-4-hydroxylase_tetra"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019773">
        <property type="entry name" value="Tyrosine_3-monooxygenase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01268">
        <property type="entry name" value="Phe4hydrox_tetr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11473">
        <property type="entry name" value="AROMATIC AMINO ACID HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11473:SF24">
        <property type="entry name" value="PHENYLALANINE-4-HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01842">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00351">
        <property type="entry name" value="Biopterin_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000336">
        <property type="entry name" value="TH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00372">
        <property type="entry name" value="FYWHYDRXLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56534">
        <property type="entry name" value="Aromatic aminoacid monoxygenases, catalytic and oligomerization domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51410">
        <property type="entry name" value="BH4_AAA_HYDROXYL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0408">Iron</keyword>
    <keyword evidence="6 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-0585">Phenylalanine catabolism</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ACT" evidence="15">
        <location>
            <begin position="33"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="Biopterin-dependent aromatic amino acid hydroxylase family profile" evidence="14">
        <location>
            <begin position="102"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="281"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="286"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="326"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000336-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR601273-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005712-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="447" mass="51454" checksum="5B1EBC20746206B9" modified="2020-08-12" version="1">MYGRQESQGPTLKEGGSYIMEGHDAAEAKNVCLIFSPEQEEAGALAKMLRIFDDHRVNLLHIESRSSTRGPGYEFMVECDGKRGTLGPAIEAIREKSNYFNIISRDYKDNEATVPWFPRRIRDLDRFANQILSYGAELDSDHPGFTDQTYRERRKYFADIAFNYKHGQPLPHVDYTPEEVNTWREVFRNLTKLYRTHACREHNHVFPLLIENCGYREDNIPQLEDVSNFLKDCTGFTLRPVAGLLSSRDFLAGLAFRVFHSTQYIRHPSKPLYTPEPDVCHELLGHAPLFADPSFAQFSQEIGLASLGAPDEFVEKLATCFWFTVEYGMCRQNGELKAYGAGLLSSFGELQYCLTDKPELREFDPAKTCEQKYPITEYQPVYFVSESFEDAIQKMINYANTIPRPFGVRYDPYTQSIEILDSVPQISNLMRNIHNEFEVLQNAFKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VMY4</accession>
    <name>A0A6E8VMY4_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Protein FAM13A</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM13 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VMY4"/>
    <dbReference type="EnsemblMetazoa" id="ACON005617-RB">
        <property type="protein sequence ID" value="ACON005617-PB"/>
        <property type="gene ID" value="ACON005617"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006780"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005617"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034170"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039102">
        <property type="entry name" value="FAM13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059029">
        <property type="entry name" value="FAM13A_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15904">
        <property type="entry name" value="FAM13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15904:SF17">
        <property type="entry name" value="RHO-GAP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26116">
        <property type="entry name" value="FAM13A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAM13A-like" evidence="4">
        <location>
            <begin position="886"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="20"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="189"/>
            <end position="215"/>
        </location>
    </feature>
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            <begin position="294"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
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            <begin position="423"/>
            <end position="458"/>
        </location>
    </feature>
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        <location>
            <begin position="499"/>
            <end position="519"/>
        </location>
    </feature>
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        <location>
            <begin position="571"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="673"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="813"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="893"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="20"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="54"/>
            <end position="102"/>
        </location>
    </feature>
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        <location>
            <begin position="112"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="205"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="298"/>
            <end position="349"/>
        </location>
    </feature>
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        <location>
            <begin position="353"/>
            <end position="370"/>
        </location>
    </feature>
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            <begin position="423"/>
            <end position="451"/>
        </location>
    </feature>
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        <location>
            <begin position="577"/>
            <end position="586"/>
        </location>
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        </location>
    </feature>
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        </location>
    </feature>
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        <location>
            <begin position="859"/>
            <end position="880"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF26116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005617-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="958" mass="105119" checksum="A3107CC9E87ED1C5" modified="2023-06-28" version="2">MRRPSLGQNEDDLSRLTTIISPTSISLSSNGQSDPVVVVDHHDVVSCGRAKAHSTSSNSSSSSSSSSSSSGSNSSSTSISHGAGNTTSNSSSNCPASNNSVSGPAGRGSVDGGSSTTSNSSNGINTSTSTSSDGVKVIATSKGKGECDDRGTAGGHGGGRDELSEALPPPTVLVNQYLPNLINSSEWQNCRKRKERQDSTSSISQDRKLIRSNSEEHLPNCQEVIRRVSSHEDFKKRSAAAAIVSVEISIDKENVIAEEPLEGEDEQQQQVFEKNLKNSADDLKKFFIGSVESVGGKEHHHHHHHSGHLAHNNHHNHHHGNHVYHHLGGHHHHLHHGGGKSQPSHHRLSPCRDILKSRRDSDSEQDGEHERRRHCERFSKTRPPPGRKSVSPRARNSSKHSKLAVKAASVGVGAVLLAGNEHFYSKHDTHPSGGGKSERRIRSSSKHREQQQKVLQDDTVMDDGVVPLSDYNDNNVLEKNHAVEEATKLIDDEEEEVVQEVQPEEPLSPREKLPWGQTYPDDTPALVCQRFADDNFEYAKRMDSVSHAKLRPVSSGNSLKLYGAHHRSAENAPPATIHHHHHHHQKATPAANNMFDERVKAINRKLGGLKKKLSQFEDRFELEHGYRPTHGDKSADAPTKSILVEIHKLRKEKNQIKADLAVFSAKVAAAAATTTATTGQKQQAATGDGGLSEDETATEKRLAKMKDTIADIEKRLSEKRDTEQRSENLELLSNEQLTEEKASVQRALLYLESMYGRPASREERDAARPLYDRYRLIKRLVNRANSISGPCGVANSQMPTILEHEALAIVGTTTPSTDISPPSATSMLQSPTDTMTTPSSSSSISASNQLPAADDSEETTATTNTTSTAGNTTSSSATENIHSMTADELWQHYDATREEKKELRRTIKDFEQQFEETTGRKMLKSDRKSIEDTYALYKQKKAKLRLIDALFKKQMQAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VN81</accession>
    <name>A0A6E8VN81_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="11">G protein alpha o subunit</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VN81"/>
    <dbReference type="EnsemblMetazoa" id="ACON005773-RB">
        <property type="protein sequence ID" value="ACON005773-PB"/>
        <property type="gene ID" value="ACON005773"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011703"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005773"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035568"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005834">
        <property type="term" value="C:heterotrimeric G-protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001664">
        <property type="term" value="F:G protein-coupled receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031683">
        <property type="term" value="F:G-protein beta/gamma-subunit complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007188">
        <property type="term" value="P:adenylate cyclase-modulating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00066">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002307">
        <property type="entry name" value="Guanine nucleotide-binding protein G(k) subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.400.10">
        <property type="entry name" value="GI Alpha 1, domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001408">
        <property type="entry name" value="Gprotein_alpha_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001019">
        <property type="entry name" value="Gprotein_alpha_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011025">
        <property type="entry name" value="GproteinA_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218:SF362">
        <property type="entry name" value="G PROTEIN ALPHA O SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218">
        <property type="entry name" value="GTP-BINDING PROTEIN ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00503">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00318">
        <property type="entry name" value="GPROTEINA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00441">
        <property type="entry name" value="GPROTEINAI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00275">
        <property type="entry name" value="G_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47895">
        <property type="entry name" value="Transducin (alpha subunit), insertion domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51882">
        <property type="entry name" value="G_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0519">Myristate</keyword>
    <keyword evidence="3 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0564">Palmitate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0807">Transducer</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="43"/>
            <end position="48"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="152"/>
            <end position="153"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="177"/>
            <end position="183"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="202"/>
            <end position="206"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="271"/>
            <end position="274"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="327"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005773-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="355" mass="40419" checksum="80019C51571443EC" modified="2020-08-12" version="1">MGCAQSAEERAAAARSRLIERNLKEDGIQAAKDIKLLLLGAGESGKSTIVKQMKCVPNLQTCLTVKAKPTVLSHTSRWLTLNNSIVMLMDSFLLLSFSPPPQCDAKMVFDVVQRMHDTEPFSEDLLLAMKRLWSDSGVQECFCRSNEYQLNDSAKYFLDDLDRLGAKDYQPTEQDILRTRVKTTGIVEVHFSFKNLNFKLFDVGGQRSERKKWIHCFEDVTAIIFCVAMSEYDQVLHEDETTNRMQESLKLFDSICNNKWFTDTSIILFLNKKDLFEEKIRKSPLTICFPEYTGGQEYGEAAAYIQAQFEAKNKSTSKEIYCHMTCATDTNNIQFVFDAVTDVIIANNLRGCGLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VN85</accession>
    <name>A0A6E8VN85_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Chloride channel protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the chloride channel (TC 2.A.49) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VN85"/>
    <dbReference type="EnsemblMetazoa" id="ACON005777-RB">
        <property type="protein sequence ID" value="ACON005777-PB"/>
        <property type="gene ID" value="ACON005777"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000253"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005777"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042050"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008021">
        <property type="term" value="C:synaptic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005247">
        <property type="term" value="F:voltage-gated chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04591">
        <property type="entry name" value="CBS_pair_voltage-gated_CLC_euk_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03684">
        <property type="entry name" value="ClC_3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3080.10:FF:000011">
        <property type="entry name" value="Chloride channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.580.20:FF:000001">
        <property type="entry name" value="Chloride channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1280.20:FF:000003">
        <property type="entry name" value="Chloride channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1280.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3080.10">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014743">
        <property type="entry name" value="Cl-channel_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001807">
        <property type="entry name" value="ClC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45711">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45711:SF6">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00654">
        <property type="entry name" value="Voltage_CLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00762">
        <property type="entry name" value="CLCHANNEL"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81340">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0129">CBS domain</keyword>
    <keyword evidence="7 9" id="KW-0868">Chloride</keyword>
    <keyword evidence="5 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="230"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="307"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="363"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="388"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="416"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="452"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="489"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="526"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="600"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="11">
        <location>
            <begin position="759"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="11">
        <location>
            <begin position="854"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="24"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="103"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="38"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="116"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="140"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005777-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="917" mass="99769" checksum="9A07BB6F1F83201A" modified="2020-08-12" version="1">MEKFPLKGTGNISSSNNLSISYAATNHPLSGDHPSYQSVGSKPGKGSPASGAERHITTTTTTSLTTAPGTVGAVPVYEADEDGMIDITPGNGGLTNPNYSYSSYSANGGRQDGVGAPSSAANSSAPSITTTAGASSNGGASIGGGEGPGGMSSPTHTRFGRDFHHSDHEGISFAGMTDTSDDIPGIGQYEDFHTIDWQRDIARDRMRHRYIVKKRQDSFWDLLKGAHDAWSGWVCVLLVGLFTGCVAGVIDIGASWMTDLKFGICPQAFWLNREQCCWSSNETSFDSGNCSQWYAWSEIFTSSREGFGAYVISYFFYIMWAMLFALLAASLVRMFAPYACGSGIPEIKTILSGFIIRSYLGKWTLIIKSVGIMLSVSAGLSLGKEGPMVHIASCIGNILSYLFPKYGRNEAKKREILSAAAAAGVSVAFGAPIGGVLFSLEEVSYYFPLKTLWRSFFCALIAAFILRSINPFGNEHSVLFYVEYNKPWIFFELVPFIGLGIIGGIIATLFIKANLWWCRFRKYSKLGQYPVTEVLIVTFITAVIAYPNPYTRMNTSELIYLLFSQCGISNQDPLCDYNRNFTDVNSAIEIAAAGPGVYKAVWLLILALAMKLIMTIFTFGMKVPCGLFIPSLALGAITGRIVGIAMEQLAYNYPKIWIFSGECSTGDDCITPGLYAMVGAAAVLGGVTRMTVSLVVIMFELTGGVRYIVPLMAAAMASKWVGDALGRQGIYDAHIALNGYPFLDSKDEFQHTTLAADVMQPKRNETLAVITQDSMTVDDIETLLKETEHNGYPVVVSKENQYLVGFVLRRDLNLALANARRIIDGITGQSLVIFTSAQPVQNLGPSPLKLKKILDMAPITVTDQTPMETVVDMFRKLGLRQTLVTHNGRLLGVITKKDVLRHVKQMDNEDPNTVLFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VNG4</accession>
    <name>A0A6E8VNG4_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Neurabin-1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Neurabin-I</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Neural tissue-specific F-actin-binding protein I</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Protein phosphatase 1 regulatory subunit 9A</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Synapse</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON005943-RA">
        <property type="protein sequence ID" value="ACON005943-PA"/>
        <property type="gene ID" value="ACON005943"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_008685"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005943"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_036020"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031175">
        <property type="term" value="P:neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06790">
        <property type="entry name" value="PDZ_neurabin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09512">
        <property type="entry name" value="SAM_Neurabin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000010">
        <property type="entry name" value="Neurabin-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000008">
        <property type="entry name" value="Neurabin-1 isoform 1-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040645">
        <property type="entry name" value="Neurabin-1/2_PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043446">
        <property type="entry name" value="Neurabin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16154">
        <property type="entry name" value="NEURABIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16154:SF6">
        <property type="entry name" value="SPINOPHILIN, ISOFORM J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17817">
        <property type="entry name" value="PDZ_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="8 16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5" id="KW-0221">Differentiation</keyword>
    <keyword evidence="6" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0770">Synapse</keyword>
    <feature type="domain" description="PDZ" evidence="19">
        <location>
            <begin position="701"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="18">
        <location>
            <begin position="1417"/>
            <end position="1482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="14"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="261"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="495"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="832"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1026"/>
            <end position="1072"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1088"/>
            <end position="1135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1151"/>
            <end position="1185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1215"/>
            <end position="1299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1321"/>
            <end position="1402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1480"/>
            <end position="1515"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="801"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="871"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="955"/>
            <end position="989"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="14"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="401"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="432"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="455"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="502"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="530"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="1026"/>
            <end position="1040"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1041"/>
            <end position="1058"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="1107"/>
            <end position="1116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1162"/>
            <end position="1176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1257"/>
            <end position="1269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1290"/>
            <end position="1299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1339"/>
            <end position="1400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00067399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00076637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00077125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00082439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005943-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1515" mass="165668" checksum="5BB3D82857DF5720" modified="2020-08-12" version="1">MCLSIRRFCIDCKGKSSRTDDEHSGEQQQQPPQQQGGVTRRTKVSSICFDVPAAGLGNRPPSIISSSTTTDEGGFNEPSPEIKAKLKPAYEFDLPLPASPPRKVPIGEDRSPVGSPAEAKLTYADLGYRVRPDGTECDEIYGEVDVYRSAKQPAVGVPLARSQPSGAAASAGVPNGGQAAAQLAEPQVVAGPATLANGRSGPTERVVYASILPSSLGDNGSTTTMATDSELAFMDGERDEAKILDFDELSEMGEKGYHSAQILLDPTRTTLSQPADDDYSTKTLPEPPSLASLRPPPIPEGPPLDLQDTLKFADASDNEQDSLLPDEMTADEAERLLSSRQRPLLSDEQAREVEQILLNNQAKAEAEEPPAVRVVAGPRQQVPSVPEPAAKPQQLPPKQAPPTTTTAAVSSTAPTADEPDWLKDVLEAPKNATSSSSTAGSSMRDKPPPPPPPTSASRNNSTTSHPEDTSIGNNTTCDLLNQTILLDSSSMLADSYVDTGDSIPSVTTTTSDSINLSKLEESAAETTGALEDEGEATQEEEGQEEEQQQQQQQQQLASGRCVAAHRQQQQPQALAEGSVSEADVSADDESNNSAAGFYIPEYPPVRCKEVYVNPDGVHFFEDGNFWMEVPGLIEAERELDDDDLRGYVKKTTKVKFSCNPMQVFSTFSVNDYDRRNEDVDPVAASAEYELEKRVEKMDVFPVELMKGPEGLGLSIIGMGVGADAGLEKLGIFVKTITDNGAAARDGRIQVNDQIIEVDGKSLVGVTQAYAASVLRNTSGLVRFQIGRERDPENSEVAQLIRQSLQADREKEERVKRQLEDYIRRNAEISEDSTLPVSANSSVSEGPVSPTTNAAESLFETEAARSSDVESLRRILQELIKYQQTCNEAELLNERVKQTERELANIKKEANNYQNMLQQSQAQYLVLEKKYSRVKKILRDYQHRERDMIQFQEYYLQHLQEKDTEYNALVKKLKDRVISLEQELQETQRKAGFPVILPYDSASLKLTPQMSRRQPPKPVFQKLETDLSDTEFSDLSPDGEGEDGKTATVERKVPATTKDDDLDVAVPQHELLDNSLNKGKSDLVARGGLAKRNLPGKRSHSNSGSDCALDESDDDEGQLTRNATGTEGSHEYSGLYSTPQIVNRKVATLKGEDSGLPVYAQVNKERSSGGGHADHHQQQQQQQQHTTIPNIYRAAVGSDNGKLNSSYGSDLNASSYDSDLGSSTDKLKDSGLSSDSWMYPSRRPKGLKGITPPLLAEQLKERLAEREGRRGSGGGLIDDGSSRDSSDDYSEINQQQRHISPAASLSQNLLVEIKKAVNEAQPKVKNILPQNLSPPGGNGPWQQQQQQQQQQGAQSTSGGTTAGSSQGPPSPSSVSSGSTSPGAYSPSRTLDLSGSTSSFSSDRIRDTHQWKNGPVEEWSNEQVCQWLLGIGLDHHIPAFMQHSVEGGALLQLDKPDFKILNVGGDDKKLLKRNIKELRRLNEKERKQNEKERREREKLFKKQEKKAEKEQKKRYQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VPJ7</accession>
    <name>A0A6E8VPJ7_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Iron-sulfur clusters transporter ABCB7, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">ATP-binding cassette sub-family B member 7, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(glutathione)4[2Fe(III)-2S] cluster(in) + ATP + H2O = (glutathione)4[2Fe(III)-2S] cluster(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:67028"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:167627"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:67029"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VPJ7"/>
    <dbReference type="EnsemblMetazoa" id="ACON006364-RC">
        <property type="protein sequence ID" value="ACON006364-PC"/>
        <property type="gene ID" value="ACON006364"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_014470"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON006364"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037903"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18582">
        <property type="entry name" value="ABC_6TM_ATM1_ABCB7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000004">
        <property type="entry name" value="ATP-binding cassette sub-family B member 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000186">
        <property type="entry name" value="ATP-binding cassette sub-family B member 7, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221:SF402">
        <property type="entry name" value="IRON-SULFUR CLUSTERS TRANSPORTER ABCB7, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="144"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="185"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="259"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="290"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="373"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="415"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="13">
        <location>
            <begin position="145"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="471"/>
            <end position="705"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00041016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00042945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON006364-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="725" mass="80342" checksum="6D5738A0CE802D43" modified="2020-08-12" version="1">MAALLHVVSKQAPQLNRNLLEGVRLSFKNHHPLSETHCRLQSRQLHVSAVAVANWYHDNRKRKHSEPWGQRRSYATQGALPEKNQQATENALLNIFQKKRKGHHPASGESIGTYDGPEITATDMIKAMATYIWPKDDAMVRKRVLISLGLLGGAKVLNVCVPFLFKMGVDNLNVLSMDTVPQAASALTISVLLGYGIARAGAAGFNELRNAVFARVAQHSIRKIATNVFLHLHNLDLQFHLSKQTGALSKTIDRGSRGINFVLTAMVFNVVPTMFELALVSSILGMKCGMAYAALSMGCVGVYSAYTLAVTQWRTKFRIYMNQAENEAGNKAVDSLINYETVKYFNNEQYEANRYDQVLKKYEDASLKTSTSLALLNFGQNAIFSVALSTIMVMAANEIAQGRMTVGDLVMVNGLLFQLSIPLGFLGSVYREVRQALLDMRTMFTLMGVQSAIQSRANAPPLDVRRETASIEFRNVGFRYAQSNEIFKDLSFTIPAGKKIAIVGGSGSGKSSMVRLLFRFFEPSTGEILINGQNIREVDLQSLRKAIAVVPQDSVLFHDTIRHNIHYGDLSKSQTELENAARLADLHDSILQWPKQYETQVGERGLKLSGGEKQRVAIARAILKNSPILIFDEATSSLDSITEQNILQALARATDNRTSICIAHRLSTVMDADEILVLENGRVGQRGTHDQLLRGGGLYTKLWDTQNRLYNIGSKKPTKPEGEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VPK9</accession>
    <name>A0A6E8VPK9_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase</fullName>
            <ecNumber evidence="3">3.2.2.6</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>NAD(+) + H2O = ADP-D-ribose + nicotinamide + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16301"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57967"/>
            <dbReference type="EC" id="3.2.2.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:16302"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SARM1 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.6" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8VPK9"/>
    <dbReference type="EnsemblMetazoa" id="ACON005901-RA">
        <property type="protein sequence ID" value="ACON005901-PA"/>
        <property type="gene ID" value="ACON005901"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000213"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005901"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033436"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044297">
        <property type="term" value="C:cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003953">
        <property type="term" value="F:NAD+ nucleosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061809">
        <property type="term" value="F:NAD+ nucleosidase activity, cyclic ADP-ribose generating"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035591">
        <property type="term" value="F:signaling adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019677">
        <property type="term" value="P:NAD+ catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034128">
        <property type="term" value="P:negative regulation of MyD88-independent toll-like receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048678">
        <property type="term" value="P:response to axon injury"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09501">
        <property type="entry name" value="SAM_SARM1-like_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09502">
        <property type="entry name" value="SAM_SARM1-like_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd24153">
        <property type="entry name" value="SARM1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000056">
        <property type="entry name" value="Ectoderm-expressed 4, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000043">
        <property type="entry name" value="Sterile alpha and TIR motif-containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10140:FF:000012">
        <property type="entry name" value="Sterile alpha and TIR motif-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039184">
        <property type="entry name" value="SARM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22998:SF1">
        <property type="entry name" value="NAD(+) HYDROLASE SARM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22998">
        <property type="entry name" value="SARM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07647">
        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13676">
        <property type="entry name" value="TIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00255">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0391">Immunity</keyword>
    <keyword evidence="5" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="9" id="KW-0520">NAD</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="SAM" evidence="13">
        <location>
            <begin position="454"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="domain" description="TIR" evidence="12">
        <location>
            <begin position="601"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="16"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="52"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="761"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="58"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="778"/>
            <end position="795"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005901-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="795" mass="89117" checksum="CC7B29AE35A6FD45" modified="2020-08-12" version="1">MGDFQQAEANNIAAHSRKVHSDHFSAENKAISQAQQKQTVSASGIFNQEKHVSSTTQSTFTMSNTSTISSSSQMSTLSSSSSAALGDDFPSFEDFERLGNTPEEIDSTIMKYSKHLANYVQNLQQTEQLKKAPTLLNHMNEIIRRAWAVPTHGHELGASLCDTLRNTGGLDILMRNCVDNDSEVQFSSARLLEQCLTTENRTHVVKNGLDKVVNVACVCTRNNATDHSRVGTGILEHLFKHSEDTCSNVIRLGGLDAVLFECRKNDVETLRHCAGALANLSLYGGSENQEAMINRKVPSWLFPLAFHNDENIKYYACLAIVVLVANKEIEAEVIKSGTLNLVEPFVTTHNPSEFAKSNLAHAHGQSKHWLQRLVPVLSSKREEARNLAAFHFCMEAGIKKEQGNTNIFKEIGAIEPLIRVASSPNAIASKFAAQALRLIGEEVPHKLSQQVPLWSTEDVREWVKQIGFGEYEQSFYDSKVDGDLLLQMQDHNLCDDIGMKNGILRIRFLRELQNLKKMADYSSRDTDGLHAFLSRIGPEYTIYTYTMLNAGVDIESLRTLDEDQLTATCGITNAIHRSRILQVIKLNESIPSFRSEQSAEKNLDVFVSYRRSNGSQLASLLKVHLELRGFSVFIDVERLEAGKFDNNLLQSIRHARHFLLVLTPDALNRCIDDVDCKDWVHREIVAALNSECNIIPIIDNFQWPEPEKLPEDMRGVCHFNGIRWIHDYQDACVDKVERFMRAEMNGRGGCGLLDHGSRLLSRQPGDATPTSAIYQRMHSNDSGKSSDKENSRNLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VRB0</accession>
    <name>A0A6E8VRB0_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">LIM zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VRB0"/>
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        <property type="protein sequence ID" value="ACON006901-PA"/>
        <property type="gene ID" value="ACON006901"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000108"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON006901"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042770"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031941">
        <property type="term" value="C:filamentous actin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001725">
        <property type="term" value="C:stress fiber"/>
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    </dbReference>
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        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051371">
        <property type="term" value="F:muscle alpha-actinin binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0061061">
        <property type="term" value="P:muscle structure development"/>
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    </dbReference>
    <dbReference type="CDD" id="cd09455">
        <property type="entry name" value="LIM1_Enigma_like_1"/>
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    </dbReference>
    <dbReference type="CDD" id="cd09461">
        <property type="entry name" value="LIM3_Enigma_like_1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000067">
        <property type="entry name" value="Uncharacterized protein, isoform Z"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050604">
        <property type="entry name" value="PDZ-LIM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24214">
        <property type="entry name" value="PDZ AND LIM DOMAIN PROTEIN ZASP"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR24214:SF38">
        <property type="entry name" value="PDZ AND LIM DOMAIN PROTEIN ZASP-RELATED"/>
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    <dbReference type="Pfam" id="PF00412">
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="6">
        <location>
            <begin position="323"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="6">
        <location>
            <begin position="383"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="95"/>
            <end position="127"/>
        </location>
    </feature>
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        <location>
            <begin position="181"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="181"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="208"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="234"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON006901-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="438" mass="47432" checksum="A1577AFF598D6F25" modified="2020-08-12" version="1">MAAPAPAPMAAAPAPAPMAAPAMAPASVPTPQAAGPRIEEVDHATLLLDSSVSKLACMSNNADYRKQASSVLKDMLEARLRKTDASVRAKNKENLQHSNGTPAPTIGHRAGGFPLANTNGKAGDEPKPVPPYTNGTAAVPSKQAAFPPNGQHDLSVYSTQITVPVAPLAASELGKVILRPKNSNNHATDMNGSQAPINGRKVSSELTSHARDRRSYVERNNNVAAPHDKKQMQRDSTPTQPQEQDPVANGSALALLKDKNPICNVCDHKIVTGPFITALGRIWCPDHFICHNANCKRPLADIGFVEEKGDLYCEYCFEEFLAPLCSKCNGRVKGDCLNAIGKQFHPECFKCTYCGKQFGNSPFFLEEGDPYCEKDWNDLFTTKCFACGFPVEAGDKWVEALNNNYHSQCFNCTSCKKNLEGQSFFAKGGRPFCKNHAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VRB2</accession>
    <name>A0A6E8VRB2_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_040226336.1">
        <property type="nucleotide sequence ID" value="XM_040370402.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VRB2"/>
    <dbReference type="EnsemblMetazoa" id="ACON007064-RA">
        <property type="protein sequence ID" value="ACON007064-PA"/>
        <property type="gene ID" value="ACON007064"/>
    </dbReference>
    <dbReference type="GeneID" id="120951602"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012428"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007064"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034009"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007064-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="64" mass="7314" checksum="397D0A376CEED472" modified="2020-08-12" version="1">MVELSSSNDPFQHGFPNAVFIIPAIVVIGLGAFFGYKLYLSLTEKERKREEKLKAKQEKSKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VRN7</accession>
    <name>A0A6E8VRN7_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">LRRCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VRN7"/>
    <dbReference type="EnsemblMetazoa" id="ACON006647-RA">
        <property type="protein sequence ID" value="ACON006647-PA"/>
        <property type="gene ID" value="ACON006647"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009248"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON006647"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042463"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050333">
        <property type="entry name" value="SLRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45712">
        <property type="entry name" value="AGAP008170-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45712:SF22">
        <property type="entry name" value="INSULIN-LIKE GROWTH FACTOR-BINDING PROTEIN COMPLEX ACID LABILE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="265"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON006647-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="349" mass="38841" checksum="C70110E7C720B7E3" modified="2020-08-12" version="1">VCCCCCCSNSAQFVDPPSGTGSRGQLMHPMIMLANAPETIFDLSFNRIRDLNPQSFARYGSVKYLYLFENMIHNVEPETFSSLTNLEAIDLSNNALISIPLELFRLPSLRNLYVSHNTLSKLDEDLPALEKPIKAPLQVLGLADCRLQRLPDLGVLPDLWQLNISSNPMKELTLGQFSPMCNLKTVELNNTQIPICPCQVLTAELVLRRTKIMFPPLYCIPLTSSEQNLCNAEAKRDIEPDQVADYHQCMEIRNSRQLDTEAKSAWMKISAAVAGCIVLFATILFCLHRRNVRRLKAKRKPKKVPLKSINRVILVPGPAGDGPESEVPPATTTGTTITAKCDKLLSDCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VTC4</accession>
    <name>A0A6E8VTC4_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Sm domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_040219551.2">
        <property type="nucleotide sequence ID" value="XM_040363617.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VTC4"/>
    <dbReference type="EnsemblMetazoa" id="ACON007326-RB">
        <property type="protein sequence ID" value="ACON007326-PB"/>
        <property type="gene ID" value="ACON007326"/>
    </dbReference>
    <dbReference type="GeneID" id="120947850"/>
    <dbReference type="KEGG" id="acoz:120947850"/>
    <dbReference type="CTD" id="134353"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007261"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007326"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038602"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005683">
        <property type="term" value="C:U7 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071209">
        <property type="term" value="F:U7 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006398">
        <property type="term" value="P:mRNA 3'-end processing by stem-loop binding and cleavage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01739">
        <property type="entry name" value="LSm11_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039267">
        <property type="entry name" value="Lsm11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034109">
        <property type="entry name" value="Lsm11_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21415">
        <property type="entry name" value="U7 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM11"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR21415:SF1">
        <property type="entry name" value="U7 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM11"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
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    </dbReference>
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        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sm" evidence="2">
        <location>
            <begin position="130"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007326-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="257" mass="29244" checksum="BF06B3E0FBCA1E1E" modified="2020-08-12" version="1">MGNSDTDSTTSESDSSGELDVGSKRFNPLKALYSVKLKVPVPKARLHDNISSLEGKQLTLGGFTEKFDEARIRQIRAAQNKPKLAENEMPQRRFLPEQGPVTRVRSIRHTKNIFIRLEKGFEGPLGQLQRWMEGRNRVRVSIRKQKGVRGHVSGVLELFDKHWNLALSDVCETWTRRKYRYSENRQHPDSMEAPRDCSERLRRLGIVLPELKVRPADGKKNVIITRKVPQLLVKGEMVVLVTLEPGEPQASTSKGTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VTC9</accession>
    <name>A0A6E8VTC9_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="11">RBR-type E3 ubiquitin transferase</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="EnsemblMetazoa" id="ACON007788-RC">
        <property type="protein sequence ID" value="ACON007788-PC"/>
        <property type="gene ID" value="ACON007788"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000096"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007788"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_029747"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071797">
        <property type="term" value="C:LUBAC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036435">
        <property type="term" value="F:K48-linked polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070530">
        <property type="term" value="F:K63-linked polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990450">
        <property type="term" value="F:linear polyubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097039">
        <property type="term" value="P:protein linear polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20337">
        <property type="entry name" value="BRcat_RBR_HOIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20351">
        <property type="entry name" value="Rcat_RBR_HOIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047540">
        <property type="entry name" value="BRcat_RBR_RNF31-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047542">
        <property type="entry name" value="Rcat_RBR_RNF31-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026254">
        <property type="entry name" value="RNF31-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032065">
        <property type="entry name" value="RNF31-UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041031">
        <property type="entry name" value="RNF31_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16004:SF2">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE LUBEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16004">
        <property type="entry name" value="RING FINGER PROTEIN 31-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18091">
        <property type="entry name" value="E3_UbLigase_RBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01485">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22191">
        <property type="entry name" value="IBR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16678">
        <property type="entry name" value="UBA_HOIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00647">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="10">
        <location>
            <begin position="1711"/>
            <end position="1940"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="9">
        <location>
            <begin position="1715"/>
            <end position="1764"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="218"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="321"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="530"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="686"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="737"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1222"/>
            <end position="1243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1271"/>
            <end position="1602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="13"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="325"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="355"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="553"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="692"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="704"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="737"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="768"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="813"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="830"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="883"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1224"/>
            <end position="1243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1271"/>
            <end position="1301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1310"/>
            <end position="1344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1347"/>
            <end position="1360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1399"/>
            <end position="1431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1442"/>
            <end position="1453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1467"/>
            <end position="1476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1486"/>
            <end position="1496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1524"/>
            <end position="1536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1557"/>
            <end position="1576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1578"/>
            <end position="1588"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007788-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="2091" mass="232919" checksum="81835146A6AB5FB2" modified="2020-08-12" version="1">MDDHQDPEDMPQGTEQLTEMQNGSTSKEEPSEMTLLLREAERYHFTAEELQAAMNHCGEKHPVQWLRSNWNKLIETVQTLATKYGHEKRENTIGTISTVEAREALRMHKGNIWRAITECIEQRQRKYREIASKGNFTREDIVTSLTAHHGNLELATIELSKTQLKPFLMRIWGPPSGADNDSGNLLLQQALQEDRTGISSEIQQFISAHVEQELLGTGPSEKVMQPSEPELPSAMEQADREDIEPEIQQLTEHENTKAPTPSLPEEDHLEEVCEAQSSTTTSNTEILKDIEALIMQMERKQESSNGTVLRNIQQLLSQLVDAEPNSRSPSATSIRSQASDQRIMSKSPIPVRNGKQPDHLPDPNRSIEDDVRDFVQENIQDILPNLVQQVRQELDAVKVNLQTKPKSLEDDIRETLMRAEYSENDYSNIHYFPFDKTPEPPVHFPERQQTSKVSVQPSVANETDEYANIQKFLERAIRNEKTTTVFDQMKRSNYLIDSSSDEARQTAESTDYYDLVSINEKLMHLFTKTPSKESQPVQQHEVASKTSHPEGSPSATKVQSHVQTAIEEATRTPQGPEEPIPQESPQMEKKGVKKRRGSRIPVNKNNYLYRRPVKSSSESDFEVLATNSKQNQSSIAAIDHVEEMRHVVENIERIEQTMDVEDAPEDEDDQEDFELVDEPIYINLPPKKESEAENVEQLEEFEDTTTMPQQASPVEESTTFVEPIKDHHTESVMQDLTQDVSTPQELPQQSVEATDAVNQPIVEEQKLQDSTPAISNTIQEPPIQPVESNTAAASIAHVVEEPKPIHSQDPAPQASNENQQQPLPAPSAEENIQTNELAITASNETNGHVQEEPQPTVIAETPVVASETIASSSAPNEAVKSEPPTNGSLPDNSNNISEADQQDDVISHSSKLANNLSELVLDTKRLIQQMKDEINSDIATFNEDDAAYEEDYYEDEYEDEWEGEEEEEEWDSNEEYDVDENGDFYEYEDDDDDGSVMFSEMTIIDSAKSRDAEQPPNEMIPVQEPPQMIMPMINMAIVRENERNSASEVSDLPLDSDFNDAMSVIEQSVGELRNMLHLTEEALGPLDLANPLHHASSVETLQNSPEQSEISEITLVPEYHNTTAEESSEPTLVADENVPMTMKDVQTLMNAKAVIGGPEYATVFKANSKLQEIETANDLAQQPPASIGTTLNTSSELPATNQGEAGLVNGTELRAAITEDASVPATSESYSVEQPSEATVNSQELQVSVESAIDDHLDHPASESLKMTNDITQTTQQSIQPESTQNQQGPGDLVDTSNNPIPVTDGDVQSLPQQPTTTNVTTEQQPPEPNANNLPNGNATSGHRTLSRASSEEPSSSFVEIQPSQVIIHTPQTETQPQVVPPEAPPRTTNIYQNVDLHPSASSISPTATTITTTSTTTTSTTTPMKSPPTRSKSKAPKLTVKVTNAASSQSEDGASPTTPPTPTGQKANAKATASKTKTESKKKSTSTTSSPTADSNGRRPSVTRKKSIGGVFGPVQTNTVKNMQKEFLNKAKESPKPSTSKVAPKPAKLVQPKAFTARSSTTPTASTSKASTPASEDASTSARSTPGLNDANEPQPGSSRDTTYMCEKLMKKKYRETCFSDEYQTTDDEDESHMTVTESERTIIKSLVKPYEPNNEPPEVQAKQLMEDGLVQTLAQAELAVQLIELRYAKDNAIWAATQCHTIDDAKDLLQKECELCLGVFPMNEIISMLKCTHTCCFECAKEYFTQEITNRSITNCNCPYCKEPDLNGPEVTEDDVLEYFSNLDILLKNIVDEEVHDLFQRKIRDRTLTKDPNFKWCVHCSSGFFARPKQRRLVCPDCGSITCASCRKAWETQHEGLTCEKFAEWKEANDPELQAEGVQRHLQTHGISCPNCKFRYSLARGGCMHFTCTQCKFEFCYGCNKPFMMGAKCSVSPYCAKLGLHAHHPRNCLFYLRDKEPRDLQNLLLMNNVPFDTEPSEQMKQELANGESTTMKCPIPLQKETPAGLMDMICSADVPEKHAGLCRTHYVEYLVGTVSKAQIDPLPILDLTDCVQELRRRGIPLPERGPWDTDEIYREMCQKLVKDKIPLDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VTR4</accession>
    <name>A0A6E8VTR4_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Amino acid transporter</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VTR4"/>
    <dbReference type="EnsemblMetazoa" id="ACON007903-RA">
        <property type="protein sequence ID" value="ACON007903-PA"/>
        <property type="gene ID" value="ACON007903"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006427"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007903"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_036964"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015501">
        <property type="term" value="F:glutamate:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005313">
        <property type="term" value="F:L-glutamate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015175">
        <property type="term" value="F:neutral L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3860.10">
        <property type="entry name" value="Sodium:dicarboxylate symporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050746">
        <property type="entry name" value="DAACS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001991">
        <property type="entry name" value="Na-dicarboxylate_symporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018107">
        <property type="entry name" value="Na-dicarboxylate_symporter_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036458">
        <property type="entry name" value="Na:dicarbo_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11958:SF111">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11958">
        <property type="entry name" value="SODIUM/DICARBOXYLATE SYMPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00375">
        <property type="entry name" value="SDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00173">
        <property type="entry name" value="EDTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118215">
        <property type="entry name" value="Proton glutamate symport protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00713">
        <property type="entry name" value="NA_DICARBOXYL_SYMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0769">Symport</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="45"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="184"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="222"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="255"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="287"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="330"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="375"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007903-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="492" mass="53514" checksum="FC5A15BA164B5B5C" modified="2020-08-12" version="1">MLIAYPGELFMRVLKLMILPLVIASLISGSSSLNAKLNGKIALRTFVYFLTTSLLNAILGTTLALIIHPGNPTAESAAHTELLRDTAPLNKAKTVSLMDSVLDLGRNIFPDNIFQAALQQAHTVYVPKELPGMVGNDTELSHTSAPVMASSSASASSSVNLMEWSAEQNVEWIRIVEYRSGTNSLGIVFFCLVFGTLLGTIGRKGYVVVQFFSAIFDVIMKMVTGVMWLTPIGISSVIAGKILSVDDVGFVMTQLAWFIFTIALGVFLYQWIILQAIYFVFLRKNPFRFYLGLVQPILTGFATASTAAALPLTFRCMNERLKIDSRITRFVLPIGCNINMDGTALFIAVASIFIAQMSNMSLNVGQVVTVVLTSTAASMSSASIPSAALVLLLIALSAIDAPINNATLLFAIDWFVDRIRTTNNLLGDCYAAAVVEHLSRNELKISDIDAYYKDDDITSDDQCEQDVNGKRKLSFPNSVILNVEVSTPVSNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VTR7</accession>
    <name>A0A6E8VTR7_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="9">SH3 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VTR7"/>
    <dbReference type="EnsemblMetazoa" id="ACON007717-RC">
        <property type="protein sequence ID" value="ACON007717-PC"/>
        <property type="gene ID" value="ACON007717"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_008198"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007717"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032226"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041602"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070161">
        <property type="term" value="C:anchoring junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd11781">
        <property type="entry name" value="SH3_Sorbs_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11782">
        <property type="entry name" value="SH3_Sorbs_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11780">
        <property type="entry name" value="SH3_Sorbs_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000001">
        <property type="entry name" value="Sorbin and SH3 domain-containing protein 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050384">
        <property type="entry name" value="Endophilin_SH3RF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003127">
        <property type="entry name" value="SoHo_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14167:SF116">
        <property type="entry name" value="CAP, ISOFORM AC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14167">
        <property type="entry name" value="SH3 DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00499">
        <property type="entry name" value="P67PHOX"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00459">
        <property type="entry name" value="Sorb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="SH3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50831">
        <property type="entry name" value="SOHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0965">Cell junction</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SoHo" evidence="8">
        <location>
            <begin position="254"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="7">
        <location>
            <begin position="582"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="7">
        <location>
            <begin position="655"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="7">
        <location>
            <begin position="780"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="348"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="18"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="35"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="47"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="141"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="184"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="197"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="208"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="238"/>
            <end position="248"/>
        </location>
    </feature>
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        <location>
            <begin position="249"/>
            <end position="258"/>
        </location>
    </feature>
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            <begin position="349"/>
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            <begin position="389"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50831"/>
        </source>
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        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007717-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="839" mass="94858" checksum="026BF13544D7E543" modified="2020-08-12" version="1">MGQESGTTLTIGPKFKAKTSSLGVWSPFNRSTEVLTKEELAERSSSKNKSNTSQQSPVWTPRSAPPSPATERREFRPIGFESPKPTRRTLTPSPADPSTVVAPPWNQPGYNPPLPISDNFKPYPIQNSASNPTLGAPVRSASASTTSQGQVTQVRFPPQQKSTISHDPTINTLLKSKDGKTATIKASETSESQQKGSTTSIIRTSSSLMTNSSRQSSAGIINPTANQKPLVGQRSEKASTSLQDNNYSQDRKMQQTAHKVDGIGPITREGMPLTLRSEIDEGNRDKWYKQMYQTLHKTQDDADYVTVRYKTRRGYPYKSSGYQSEPEPNYDSDYTVKYSTLDRRRTPIGLSSTSYSKFNTLQNKPTTPQQYHQQSIKSVHSSYKNQPGKIEDYIPGRSSISEKESKEQLEHQKLTTSKTYTEGNLSRALKEQGYESDSTLVFRKRDPPASAALSPVEQKQYYKNMQAGGEVPLQGFRKPAPERPKESPRRYIESDVNIHYKTPIRFEYKEPIPDDELAYRQAEHMRRVYQEERRRKYIHELEDMNARRHMDNILPSQKSPIPLNRYDDFPADLSPKPLIVQQPKTIARALYNFQGQSVRELSFRKGDIIYLRRQIDKNWYEGEHNATVGLLPANYIEILTRDNVNVKQPLPKKPTREGKARAKFNFTAQTAVELSLLKGELVTLTRRVDDNWFEGKIGSKKGIFPVSYVEILTDIDGEESYDIEPIVSRPQSALGVTSQTSQALTTHFDNSHTNGRISPGIVRETKTIQKTEVLHVDTSNEPISYRALYNYKPQNTDELELLEGDVVYVLEKCDDGWYVGTSARTGCFGTFPGNYVNKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VTS5</accession>
    <name>A0A6E8VTS5_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VTS5"/>
    <dbReference type="EnsemblMetazoa" id="ACON007401-RB">
        <property type="protein sequence ID" value="ACON007401-PB"/>
        <property type="gene ID" value="ACON007401"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007401"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007401-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="82" mass="8894" checksum="4B2B9F03F2D32A70" modified="2020-08-12" version="1">MCYGAIPTLDQEGYLRGEAIGNFQINRHPSRTLSAVTRSVLPQVRLFRVPVPDNTPSTVTTPVNGSLPPAPDTRPPAANQRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VU36</accession>
    <name>A0A6E8VU36_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein kinase C</fullName>
            <ecNumber evidence="2">2.7.11.13</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.13"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.13" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8VU36"/>
    <dbReference type="EnsemblMetazoa" id="ACON007587-RB">
        <property type="protein sequence ID" value="ACON007587-PB"/>
        <property type="gene ID" value="ACON007587"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_003661"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007587"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030620"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004697">
        <property type="term" value="F:diacylglycerol-dependent serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11623">
        <property type="entry name" value="HR1_PKN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11625">
        <property type="entry name" value="HR1_PKN_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05589">
        <property type="entry name" value="STKc_PKN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.160:FF:000001">
        <property type="entry name" value="Putative serine/threonine-protein kinase N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000058">
        <property type="entry name" value="Putative serine/threonine-protein kinase N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000038">
        <property type="entry name" value="serine/threonine-protein kinase N2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.160">
        <property type="entry name" value="HR1 repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011072">
        <property type="entry name" value="HR1_rho-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036274">
        <property type="entry name" value="HR1_rpt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02185">
        <property type="entry name" value="HR1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00742">
        <property type="entry name" value="Hr1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46585">
        <property type="entry name" value="HR1 repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51860">
        <property type="entry name" value="REM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="REM-1" evidence="17">
        <location>
            <begin position="12"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="REM-1" evidence="17">
        <location>
            <begin position="108"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="873"/>
            <end position="1132"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="16">
        <location>
            <begin position="1133"/>
            <end position="1200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="93"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="257"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="431"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="593"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="756"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="257"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="433"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="476"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="595"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="605"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="635"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="902"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00005490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00012429"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00047272"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00047470"/>
        </source>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01207"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51860"/>
        </source>
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        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007587-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1200" mass="131331" checksum="EBD8803EFD98E47B" modified="2020-08-12" version="1">MQSDARDPILSTVIPSGPEFTANDKLLISLEKQLSIETKVKNGAENMIQSISSGHHGRDKKLLAEAHQMLADSKAKIEYLRLKILKVRQNKLSSKGSEENGGESANAAKQPQELEASLDDRIEELRHRLRIEAAVVDGAKNVIRTLQSTKTIDKKALQEAQGRLSESSRKLDLLRKALELRRLELPPDCPTAQQLKAELQNVQASSPGPVQYTMRNSFRGSAQGRPAHSQQSFSRCAAVTGKLEVRLMGCQDLLEDVPGRSRRDKDAGSSSPGDLKSFVKGVTSRSSSKSYSVKDETSSEIMAVIKLDNITVGQTSWRPCSQQAWDQRFSIDLDRSRELEIGVYWRDWRSLCAVKFLRLEEFIDDIRHGMALQLEPQGVLFAEIKFLNPMISRKPKLQRQRMIFNKQQVKNIPRAKQMNINVATWGRLLKRNTTSSGSSSGPTTAGAAGAGTPSPGSATAPMAGMMANQTQHGSAGTTTPSTPGSYDPLQDDPVETLGESADHNVVGLGGGRPLGRYPRSRRTAGKPLVPLPAEPGHTDGGAARSTATVHQHAVQLPAAEKATDAITALEYALSKFDFLHQHDSLGGVSLYDKQPPLPVTPPPSSSVASSGYINNCDSTNNNNNSSNYIEDDEVPPLPATPPPVLRSTSQISQPAGALAAGATSPAVLQKSLSGDLLRPVLSMPPPVLYGGGPIVEEPPSPAPSQYQQQYAPFPEDDLTYDNIIELTIGGNVVPPPLPPHHHHPSATLMSQQASLAAAAALQAQQQQQLYQQQQQQQQLLLQQQLQQQQQQPAVVPPSLLAQQHAAAAGQASSSGMVDVGAASGAVGPPVNRRPAGGPARALQYRDSAYESRRQSQAGALAGAPGAGMCMDSFRLLSVLGRGHFGKVILAQYKHTGEYFAIKALKKGDIIARDEVESLLSEKRIFEVANTMRHPFLVNLFSCFQTDQHVCFVMEYAAGGDLMMHIHTDVFSEPRAVFYAACVVLGLQYLHESKIIYRDLKLDNLLLDTEGYVKIADFGLCKEGMGFGDRTGTFCGTPEFLAPEVLTETSYTRAVDWWGLGVLIFEMLVGESPFPGDDEEEVFDSIVNDEVRYPRFLSLEAIAIMRRLLRKNPDRRLGSSERDAEDVKRQAFFRNIVWDDLLLKKVKPPFVPTVRSAEDVSNFDEEFTSEKPALTPPKDPRVLTEVEQTYFKDFTYMADWC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VU38</accession>
    <name>A0A6E8VU38_ANOCL</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VU38"/>
    <dbReference type="EnsemblMetazoa" id="ACON008014-RA">
        <property type="protein sequence ID" value="ACON008014-PA"/>
        <property type="gene ID" value="ACON008014"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001942"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008014"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_036081"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026192836" evidence="1">
        <location>
            <begin position="16"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008014-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="103" mass="10470" checksum="56FFF50F8D69FFB6" modified="2020-08-12" version="1">MKFLVLCTLVAGAAAGYSASTYRDGKATGLYNQVPAGVDYADKFPHGNTGYNGGYTTHFSGPNGAGVAVGTGTGSFAPGFAGFPGPTVDFNNFFQGIQANFAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VU39</accession>
    <name>A0A6E8VU39_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Mitochondrial Rho GTPase</fullName>
            <ecNumber evidence="14">3.6.5.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Mitochondrial GTPase involved in mitochondrial trafficking. Probably involved in control of anterograde transport of mitochondria and their subcellular distribution.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Mitochondrion outer membrane</location>
            <topology evidence="1 14">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the mitochondrial Rho GTPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.-" evidence="14"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8VU39"/>
    <dbReference type="EnsemblMetazoa" id="ACON007998-RB">
        <property type="protein sequence ID" value="ACON007998-PB"/>
        <property type="gene ID" value="ACON007998"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010090"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007998"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_029370"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001667">
        <property type="term" value="P:ameboidal-type cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022412">
        <property type="term" value="P:cellular process involved in reproduction in multicellular organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003006">
        <property type="term" value="P:developmental process involved in reproduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035099">
        <property type="term" value="P:hemocyte migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035006">
        <property type="term" value="P:melanization defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Miro1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01892">
        <property type="entry name" value="Miro2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Mitochondrial Rho GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Mitochondrial Rho GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
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        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013566">
        <property type="entry name" value="EF_hand_assoc_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013567">
        <property type="entry name" value="EF_hand_assoc_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021181">
        <property type="entry name" value="Miro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020860">
        <property type="entry name" value="MIRO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08355">
        <property type="entry name" value="EF_assoc_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08356">
        <property type="entry name" value="EF_assoc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037488">
        <property type="entry name" value="Mt_Rho_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51423">
        <property type="entry name" value="MIRO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-0106">Calcium</keyword>
    <keyword evidence="12 14" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13 14 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7 14" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="6 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="599"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="domain" description="Miro" evidence="17">
        <location>
            <begin position="9"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="16">
        <location>
            <begin position="310"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="Miro" evidence="17">
        <location>
            <begin position="421"/>
            <end position="584"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR037488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007998-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="630" mass="71497" checksum="7FEC68A5708D810B" modified="2020-08-12" version="1">MVAWSVSHKRHVRILLVGDQGVGKTSLILSLVSEEFPEDVPLKAEEITIPADVTPEQVPTNIVDYSAVEQSDEALAEEIRKAHVVCIVYSVDCEETLDGITERWLPMVQKCSEMERKPVVLVGNKIDLVDYSTIDHVLSIMEDYPEVESCVECSAKTLHNISEMFYYAQKAVLHPTAPLYIMEEQDLTEACKKALVRIFKVCDIDGDGLLNDYELNHFQRRCFNAPLQPQVLDEVKAVLMKNTPDGIRDDSVTLSGFLFLHCLFIQRGRNETTWAVLRRFGYNESLAMSDEYLHPPVKIPPGSSTELSHRGQQFLVSLFERSDRDGDGALSPTEFQKLFSACPSPPFSTDIKRTIPTNENGWPTLHGWLCRWSLMTLVDVNKTLEYLAYLGFNVHENESQLAAIHVTRERRIDLAKKQNSRTVYMCHVIGAKEAAKTTFCRAFLAQDMKRLTDRDIRHSNRYAINTVQVYGQEKYLVLRDVDARLVLDPLQPSEVNCDVACLVYDVGNPKSFEYIARIYIKYFAESKIPVLIVGTKADLEEVRQEYLLQPPDFCHKYKLLQPQFFSVKHNKKDVYTKLATMAAFPHLKQFGLMSSDPLLWWKAGLGIAAATIVGFFVVKAFHGPGSSVTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VU47</accession>
    <name>A0A6E8VU47_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Neurexin-4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the neurexin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VU47"/>
    <dbReference type="EnsemblMetazoa" id="ACON007545-RB">
        <property type="protein sequence ID" value="ACON007545-PB"/>
        <property type="gene ID" value="ACON007545"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001685"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007545"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032696"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070161">
        <property type="term" value="C:anchoring junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00057">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00110">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000016">
        <property type="entry name" value="Contactin-associated protein-like 4 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000421">
        <property type="entry name" value="FA58C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001791">
        <property type="entry name" value="Laminin_G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003585">
        <property type="entry name" value="Neurexin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050372">
        <property type="entry name" value="Neurexin-related_CASP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15036:SF91">
        <property type="entry name" value="NEUREXIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15036">
        <property type="entry name" value="PIKACHURIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00754">
        <property type="entry name" value="F5_F8_type_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02210">
        <property type="entry name" value="Laminin_G_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00294">
        <property type="entry name" value="4.1m"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00231">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00282">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01286">
        <property type="entry name" value="FA58C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50022">
        <property type="entry name" value="FA58C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50025">
        <property type="entry name" value="LAM_G_DOMAIN"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0965">Cell junction</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 11" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0732">Signal</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026075431" description="Neurexin-4" evidence="13">
        <location>
            <begin position="28"/>
            <end position="1274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1208"/>
            <end position="1228"/>
        </location>
    </feature>
    <feature type="domain" description="F5/8 type C" evidence="14">
        <location>
            <begin position="40"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="15">
        <location>
            <begin position="179"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="15">
        <location>
            <begin position="365"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="533"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="15">
        <location>
            <begin position="787"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="954"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="15">
        <location>
            <begin position="994"/>
            <end position="1174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007545-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="19">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1274" mass="144462" checksum="7C6C4ABEB10B7889" modified="2020-08-12" version="1">MLKSFRGMLPLGQTVWLLLITVSIISAEYYQKDSYSEYDCNEPLLEHAVLSATSQLRERGPENARLNGGYSWTAQQSDFDQQFIIDLGDVRNITRIETQGRPHSNEYLTEYSISYGFNGLDYIDYREPGGNTKGNRDGDSVRMNSFEVPIIAQWVRINPTRWQNRISLRAELYGCRYESESIYFNGTGLVRYDLLRDPIAATRESIRFRFKTAHPNGVLLYSRGTQGDYFALQIKDNRMVLNVDLGAKIMTSLSVGSLLDDNIWHDVVISRNRRDIIFSVDRVIVQRRIKGEFDKLNLNREFYIGGVPNLQEGLVIQHNFTGCIENLHFNATNFIREMKDAFYDGEHLRYKMTHVTYNCPEPPINPVTFLTRGSHAKLKGYYSSKQFNVSFAFRTYEEKGLMLHHDFLQGSVQVFLEEGKVKVRLKEDNYEHNPGTILDNYEEQFNDGNWHHMMLTIKKNSLVLSIDDRPMETTKLIDITTGTLYYIGGGKTKDGFVGCMRSFAIDGNYRIPTDWKEEEYCCKGEILFDACHMVDRCNPNPCKHSGICKQNSMEFTCDCAGTGYAGAVCHTPMNALSCQAFKNVHDVKQRQRIEIDVDGSGPLAPFPVTCEFYSDGRVVTVLSHSSEHTTRVDGFAEPGSFEQNIIYEADLPQIEALLNRSTECWQTLTYACRSSRLFNSPSDPENFRPYAWWVSRHNQPMDYWAGALPGSRKCQCGVVGDCIDPTKWCNCDANSLDWQEDGGDIREKEYLPVRGLRFGDTGTPVDEKLGRYTLGPLRCSGDSLFNNEVTFRIADATIDLPPFDMGHSGDIYFEFKTTIENAVLLHARGPTDFIRLDIVGGTKLLFEYQAGTGTQKVYVEMSNKLNDDRWHSVSVERNRKEARLVVDGSTKAEVREPPGPVRALHLNSTLTIGARLDYRDGYVGCIRALLLNGEPVDLRSYAERGLYGVSVGCVGRCESSPCLNNGTCTERYDGFTCDCRWSSFKGPICADEIGVNMRSSSMIKYDFQGAFRSTLSERIRVGFTTTNPKGFLIGLFSNITQEYLTLMVSNSGHLKVVFDFGFERQELIYPVKHFGLGQYHDVRFSRKNSGSTVVLSVDNYEPQEYHFDIKGSADAQFNNVEYMYIGKNESMKDGFVGCISRVEFDDIYPLKFLFQENPPPNVRSLGTPLTEDFCGVEPVTHPPIEIETRPPPLIDEDKLRDAYGPDTAILGSVLAIILLLIILMAILIGRYMNRHKGEYLTQEDKGAEAALDPDEAVVQSTTGHQVTKRKEFFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VVG1</accession>
    <name>A0A6E8VVG1_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ral GTPase-activating protein subunit alpha/beta N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="EnsemblMetazoa" id="ACON007925-RB">
        <property type="protein sequence ID" value="ACON007925-PB"/>
        <property type="gene ID" value="ACON007925"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007291"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007925"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038881"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051056">
        <property type="term" value="P:regulation of small GTPase mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11210">
        <property type="entry name" value="Rap/Ran-GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039930">
        <property type="entry name" value="RALGAPB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035974">
        <property type="entry name" value="Rap/Ran-GAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046859">
        <property type="entry name" value="RGPA/RALGAPB_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21344">
        <property type="entry name" value="RAL GTPASE-ACTIVATING PROTEIN SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21344:SF1">
        <property type="entry name" value="RAL GTPASE-ACTIVATING PROTEIN SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20412">
        <property type="entry name" value="RALGAPB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111347">
        <property type="entry name" value="Rap/Ran-GAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ral GTPase-activating protein subunit alpha/beta N-terminal" evidence="2">
        <location>
            <begin position="142"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="368"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="445"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1277"/>
            <end position="1349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="377"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="460"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1290"/>
            <end position="1322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007925-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1501" mass="166770" checksum="AB9FE73A193C0CDA" modified="2020-08-12" version="1">MYSEWASLYPVIAENINNAQTQSVLGKFSTVGGRDVAAVVIKQLASTLGITNNAEPSNLHTDQEVQWCMDVICHGLSLPLSEHDIIKDGVNIYCEWISAVLPQTKLSVPRPIIDDPNTYVRKIIKHLYNLFVPRPGEGTDTINRQAVLCHRVLRTLQDTAQNSQILTVETWEALLLFLLAINEILLAPPIVKDDVGDQLCERVLSVLFEVWLLACSRCFPSPSLWKTFQESCAAWRHRIALVEQWNRVNLVLTAKLLEFTYGPAFPEMKIADEDVHLVPDGMTNDGIAQAWIRFLKILGAPTDLCCPQVISRTPQFIQAVLLNADGIEPQHHPCLLSLPQIFLKTMKGIAGLVDAFLGFTKGSFSGLTSSRSTSSSHPNNVPQSASLPSTGFSSMLSLCQENRYPLAANRPRCNSILHLFGEWLFDAAHIGGDTWMQNTKKRAAEAKRRPSSMIMDNRKGSLSQPPSLSEVNDVSPGLTIDKYESGKAEALGALCRIFCAKKTGEEILPVYLARFYMALHQGLKTNDSRECMETLASILYNSSDLFRIDLEGIQVLLPSFISALELILPEKDLRMRSQNVLINKTELRRAAINILLSMLALPLHFQTLPIRDIMGGPNDRSITFLHLKPRLINILMNALQVETDPQNTHMLLGGLHLCVQDSVTFEETENGSAEALHSLHPTAETSNLLSSACSEKSAYSMNSANSSIGGHSTATLSNEPSSLPAFDDFSSDIIHELELSSSAIYDSAHALFVRATYLVCHRLISSWKTDLNVSLAALELLTGLAGMRVKDSDVLECKRAVKWICDYICYQCSRPPPAHIKDLHSTIVAAFQCTSAWLMNHPYLLQDKECLTIVLEVVELGISGSKSVGKPGEPVKYKDEKELKPASMRVRDAAENMLTMILEQIDYFPNECGKQSLSSLLDEVVLAKHSNTSGEYVGELPQEQAIKKFKYFVTENSTVLALFEEPLGNDQDPQPTVTILIRGPFGRHAWTMQLRHLSRSKSGTKYHAPNPGRPVPMNDIMMRQEMEYKYFPDSVDRIPPCVVDYSIPTLDSAEQKIGNQSTKQLARLVEQQVGYEKLSWAETECSVDGLGHAQEASPPSVCHEFQAARLFLSHFGFLTIGQNNASGQKLDGSAPLLTTLDTSKPEFCQDLRMLDKMSPRSCDTIHVFYVKAGQSTEAEIIGNMDPENLSSIEPHFWRMLYTIGWPVAVQEHAGWTGFIGSSWKIPRDNKPCAQQGEQKTGSPEEWNLNGEKTVLYWADVSSEMAIVVPNRNNKVDITTGEDTGAGDGSSCASTTYERSVSEIQPRSSSVSTNQPRQYSLDSESARYGSMSKSADPIPPVRRRTGATKPSTLYTAPSAKILLVWLESYEDHLTFPIDGLLPYTRAGYSTQHGTVASIPSSECFIIYLHALSSSLLRVKLQGPVGRMNFAIPLIDGMVVSKRVIGSLIRQTACNMAKRKRLDNDSYQPPHVRRRIKIQEMMQRYKKDLTEPEMLADLFKSSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VVG8</accession>
    <name>A0A6E8VVG8_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 16">Nicotinate phosphoribosyltransferase</fullName>
            <ecNumber evidence="5 16">6.3.4.21</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the first step in the biosynthesis of NAD from nicotinic acid, the ATP-dependent synthesis of beta-nicotinate D-ribonucleotide from nicotinate and 5-phospho-D-ribose 1-phosphate. Helps prevent cellular oxidative stress via its role in NAD biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15 16">
            <text>5-phospho-alpha-D-ribose 1-diphosphate + nicotinate + ATP + H2O = nicotinate beta-D-ribonucleotide + ADP + phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:36163"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32544"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57502"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.21"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 16">Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from nicotinate: step 1/1.</text>
    </comment>
    <comment type="PTM">
        <text evidence="16">Transiently phosphorylated on a His residue during the reaction cycle. Phosphorylation strongly increases the affinity for substrates and increases the rate of nicotinate D-ribonucleotide production. Dephosphorylation regenerates the low-affinity form of the enzyme, leading to product release.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 16">Belongs to the NAPRTase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.21" evidence="5 16"/>
    <dbReference type="RefSeq" id="XP_040236927.1">
        <property type="nucleotide sequence ID" value="XM_040380993.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VVG8"/>
    <dbReference type="EnsemblMetazoa" id="ACON008516-RB">
        <property type="protein sequence ID" value="ACON008516-PB"/>
        <property type="gene ID" value="ACON008516"/>
    </dbReference>
    <dbReference type="GeneID" id="120958297"/>
    <dbReference type="CTD" id="93100"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009900"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008516"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033181"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00457"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004516">
        <property type="term" value="F:nicotinate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034355">
        <property type="term" value="P:NAD+ biosynthetic process via the salvage pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01570">
        <property type="entry name" value="NAPRTase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.140.10:FF:000002">
        <property type="entry name" value="Nicotinate phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.140.10:FF:000004">
        <property type="entry name" value="Nicotinate phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000155">
        <property type="entry name" value="Nicotinate phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000173">
        <property type="entry name" value="Nicotinate phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.140.10">
        <property type="entry name" value="nicotinate phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041525">
        <property type="entry name" value="N/Namide_PRibTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041619">
        <property type="entry name" value="NAPRTase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040727">
        <property type="entry name" value="NAPRTase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007229">
        <property type="entry name" value="Nic_PRibTrfase-Fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006405">
        <property type="entry name" value="Nic_PRibTrfase_pncB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036068">
        <property type="entry name" value="Nicotinate_pribotase-like_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01513">
        <property type="entry name" value="NAPRTase_put"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11098">
        <property type="entry name" value="NICOTINATE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11098:SF1">
        <property type="entry name" value="NICOTINATE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04095">
        <property type="entry name" value="NAPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17956">
        <property type="entry name" value="NAPRTase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17767">
        <property type="entry name" value="NAPRTase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000484">
        <property type="entry name" value="NAPRT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51690">
        <property type="entry name" value="Nicotinate/Quinolinate PRTase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54675">
        <property type="entry name" value="Nicotinate/Quinolinate PRTase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 16" id="KW-0436">Ligase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13" id="KW-0464">Manganese</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9 16" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 16" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nicotinate phosphoribosyltransferase N-terminal" evidence="18">
        <location>
            <begin position="33"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="Nicotinate/nicotinamide phosphoribosyltransferase" evidence="17">
        <location>
            <begin position="332"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="domain" description="Nicotinate phosphoribosyltransferase C-terminal" evidence="19">
        <location>
            <begin position="437"/>
            <end position="545"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00021569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU365100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF04095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF17767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF17956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008516-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="560" mass="62774" checksum="DABC8DC1194A1AEB" modified="2020-08-12" version="1">MSSLNRELSTDSAFAAGRNSWNRHYHNSVVQPLLTDLYQITMAYAYWKSGKIDDHAVFDLFFRTNPFQGEFTIFAGLEECLKFLDSFHYSETDIEYLKHALPQGIEDEFFEYLSQLTAKDVTLYAIEEGSVAFPRVPLIKVAGPLIIVQLLETTLLTLVNYASLMATNAARYRMVAGKHIHLLEFGLRRAQGPDGGLSASKYSYIGGFDGTSNVLAGKLFNIPVKGTHAHAYITSFTGIDELKTRVLQHKEDGTTRDLLELAMEHRAAIASVLDVSTDESSEGELAAMVSFAIAFPDGFMALVDTYDVKRSGLLNFCAVALGLNDQGYRAIGIRIDSGDLAYLSCLARETFERIAERFKLPWFSKLTIVASNDINEDTILSLNEQGHKIDCFGIGTHLVTCQRQPALGCVYKMVEINNQPRIKLSQDVGKVTMPGSKNVFRLYGADGHALIDLLQRVDENPPEVGQKVLCRHPFQESKRAYVIPTQVEPLYRVYWAEGRVAQVLPSLEEVRERVQASLRTLRQDHKRTLNPTPYKVAVSDNLYNFIHELWLQNAPIGELS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VVH6</accession>
    <name>A0A6E8VVH6_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Reticulon-like protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Endoplasmic reticulum membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_040233097.2">
        <property type="nucleotide sequence ID" value="XM_040377163.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VVH6"/>
    <dbReference type="EnsemblMetazoa" id="ACON007940-RB">
        <property type="protein sequence ID" value="ACON007940-PB"/>
        <property type="gene ID" value="ACON007940"/>
    </dbReference>
    <dbReference type="GeneID" id="120955923"/>
    <dbReference type="CTD" id="33721"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010942"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007940"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042983"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.2480:FF:000001">
        <property type="entry name" value="Reticulon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.2480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003388">
        <property type="entry name" value="Reticulon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046964">
        <property type="entry name" value="RTN1-4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45799">
        <property type="entry name" value="RETICULON-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45799:SF2">
        <property type="entry name" value="RETICULON-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02453">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50845">
        <property type="entry name" value="RETICULON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="35"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="141"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="Reticulon" evidence="7">
        <location>
            <begin position="22"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007940-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="213" mass="23809" checksum="14F9A047950B6C2E" modified="2020-08-12" version="1">MAKNALAEYLVLEASDFTKDGLESLIYWRDVKKSAVVFGGGLAILLAMSLFSLISVFSYLSLLLLFGTISFRIYKNVLQAVQKTSEGHPFKDYLELDLTLSQEKVQQLATVAVAHVNALVTGLRRLFLVEDLVDSIKFGVLLYWLTYVGALFNGMTCVMIGFVALFTLPKVYENNKQSIDTHLEMVRSKIQEITEKVKAAVPLGKKTETEKTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VVH7</accession>
    <name>A0A6E8VVH7_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="14">JmjC domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">Ngousso</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the UTX family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VVH7"/>
    <dbReference type="EnsemblMetazoa" id="ACON008509-RA">
        <property type="protein sequence ID" value="ACON008509-PA"/>
        <property type="gene ID" value="ACON008509"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009326"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008509"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039385"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044666">
        <property type="term" value="C:MLL3/4 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071558">
        <property type="term" value="F:histone H3K27me2/H3K27me3 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1370:FF:000001">
        <property type="entry name" value="lysine-specific demethylase 6A isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.20:FF:000002">
        <property type="entry name" value="lysine-specific demethylase 6A isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051630">
        <property type="entry name" value="Corepressor-Demethylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046941">
        <property type="entry name" value="KDM6_GATAL_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048562">
        <property type="entry name" value="KDM6A_B-like_C-hel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048560">
        <property type="entry name" value="KDM6A_B-like_GATAL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14017:SF1">
        <property type="entry name" value="LD02225P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14017">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02373">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21322">
        <property type="entry name" value="KDM6_C-hel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21326">
        <property type="entry name" value="KDM6_GATAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="7" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="JmjC" evidence="13">
        <location>
            <begin position="596"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="26"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="122"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="251"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="522"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="898"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="940"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="56"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="122"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="265"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="284"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="309"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="325"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="522"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="543"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="946"/>
            <end position="957"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008509-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="963" mass="102960" checksum="FCEB9117404EFC8E" modified="2020-08-12" version="1">MVRDHAVLLGNAQSEVRPACRIKGAQYHPGQQPQQQQVGSNNGLPNKRFKQEDGRPGSGPAGGGVVGTVAGQPVPPFYLNQQQVLQLQFLQNQNNLTAQQQAMLQTLTNQYRLMREHQLRLQQQRSQQLQQQQQQAAGFQGQPPQQGGNGGFGLAAGNKTPQSGIVPQTGFVNDGHFSPATGQSQQTAGMPYKSAGTYAPSGFTVPSGQAAATTPGGFTQITSGSNHQADIAIQSILDDKATFAESLLKQLSSPTGDGQDVSDPSTTTTTSSSSTKSPPIKAEATSTTTAAAAGSGAKRQLLTGSKQQHHPDIKLEPVVKLEKLPSPSSGGLDGSSSSPSSSSSVGGDFKISMSAKDIQQLVRKQRQAGELKDVPAVCSVLSADAPPPCPPDCPPTRLTREQLQPPTPSVFLENKKDAFSPQLQEFCLKHPIAVVRQLGAALKLDLGLFSTKTLVEANPDHTVEVRTQVHQSPDENWDGNKNSKVWACISHRSHTTIAKYAQYQASSFSDKIKEERDKLAGISTASTNSDSDSKDSISNSGSACGGGGGTGATSGGGTGTGGGNSSAAGNGKRKKCKNGNKMLRFGTNVDLSDERKWKAQLQELQKLPPFARVVSAANMLSHVGHMILGMNTVQLYMKVPGSRTPGHQENNNFCSININIGPGDCEWFATPDSYWGGIQALCEKNNINYLHGSWWPALEDLYAENIPVYRFTQRPGDLVWVNAGCVHWVQAIGWCNNIAWNVGPLTARQYQLAVERYEWNKLESYKSIVPMVHLSWNLARNIKVSDPKMFESIKTCLMQTMKHCMQVLEYVKSLHIEVRFHGRGKNEASHYCGQCEVEVFNVLFIREQEKRHIVHCMGCARKQSPGLQGFVCLEEYTLDELMQVYDAFVLHTPPPPLPPIAAPPAQSPQHVQQPASSPAGALINVSSSSTAVAAATSCSSSSASAAGGGAGAGGGTGSVPVAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VW77</accession>
    <name>A0A6E8VW77_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="14">C2H2-type domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VW77"/>
    <dbReference type="EnsemblMetazoa" id="ACON008827-RB">
        <property type="protein sequence ID" value="ACON008827-PB"/>
        <property type="gene ID" value="ACON008827"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005467"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008827"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033947"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001666">
        <property type="entry name" value="MDS1 and EVI1 complex locus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000512">
        <property type="entry name" value="zinc finger protein 197 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000688">
        <property type="entry name" value="zinc finger protein 197 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24381">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24381:SF390">
        <property type="entry name" value="ZINC FINGER PROTEIN 37 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="116"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="391"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="419"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="447"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="475"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="503"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="531"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="248"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="189"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008827-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="601" mass="66911" checksum="78FEDE00EEC3FFDD" modified="2020-08-12" version="1">MFTANNIQTATGTPIGIQYQTATATDINKGTKLEGQKTNQQAPEFSTFYANALSTVNLAQLSDDSKTVQYLQPFGYANCALVNQMPIPNGVTGITVDGRQLVVNKPITNTISNISFKCDVCGLMFNHLTLLNHHKRTHNQDGETTSDAITVVTQAQNLVQAQNLISETGQNLGQIQIVATEALDPAPQQQQLQHAQEQQQQQQQQQQQQQQAQAQAQAQAQQQAQQQAQQQAQQQAQQQQQQQQQVTTHTTKADKSQKCISCNGPIHSNPKRKGPKLIRCETCISNDGAQPVSRSTTQIFVAPDGDIKFEIGEIPNASNSNSSMDSLMPGQMHPIKAEHALVPQQNAHPVKKRNLATVTKCNKCNGSGVIIVGGQKKLIQSSMHQQPEKPFTCNTCGGRFSRYSSLWSHKKLHTGEKNYKCNVCGIAFAKAVYLKNHMRIHTGEKPYKCGTCGMQFSQSPHLKNHERTHSGEKPYVCEVCDKGFARHATLWNHRRIHTGEKPYKCNRCQSAFSQAAHLKNHEKVHSGLKPFKCDICSAAFADRFALKRHRGIHEKYGQTTPLHQNQQQQQQTAQVVTQQVVHKEEIIEMEEKPREVIITGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VW89</accession>
    <name>A0A6E8VW89_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="18">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="23">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14 18">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 17">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="18">Membrane</location>
            <topology evidence="18">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 18">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="18"/>
    <dbReference type="RefSeq" id="XP_040232789.2">
        <property type="nucleotide sequence ID" value="XM_040376855.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VW89"/>
    <dbReference type="EnsemblMetazoa" id="ACON008184-RB">
        <property type="protein sequence ID" value="ACON008184-PB"/>
        <property type="gene ID" value="ACON008184"/>
    </dbReference>
    <dbReference type="GeneID" id="120955741"/>
    <dbReference type="KEGG" id="acoz:120955741"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007056"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008184"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031361"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140326">
        <property type="term" value="F:ATPase-coupled intramembrane lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07541">
        <property type="entry name" value="P-type_ATPase_APLT_Neo1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000009">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006539">
        <property type="entry name" value="P-type_ATPase_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032631">
        <property type="entry name" value="P-type_ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="ATPase_P-type"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF5">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE"/>
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    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
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    </dbReference>
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        <property type="entry name" value="Cation_ATPase"/>
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    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
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        <property type="entry name" value="PhoLip_ATPase_N"/>
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    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
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    <dbReference type="SFLD" id="SFLDG00002">
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    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 16 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0445">Lipid transport</keyword>
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    <keyword evidence="6 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 16 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 18" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="75"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="112"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
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            <begin position="316"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
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            <begin position="341"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="935"/>
            <end position="955"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="961"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1013"/>
            <end position="1033"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1039"/>
            <end position="1061"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1068"/>
            <end position="1089"/>
        </location>
    </feature>
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        <location>
            <begin position="1101"/>
            <end position="1119"/>
        </location>
    </feature>
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            <begin position="60"/>
            <end position="116"/>
        </location>
    </feature>
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            <begin position="155"/>
            <end position="291"/>
        </location>
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            <begin position="901"/>
            <end position="1128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="484"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="508"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="active site" description="4-aspartylphosphate intermediate" evidence="15">
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            <position position="401"/>
        </location>
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            <position position="401"/>
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    <sequence length="1135" mass="127378" checksum="9C213C21AE9EB6B7" modified="2020-08-12" version="1">MEDAPPHGHIPSAPVTKETIPLIEKAPKTRWFSCWCWAWRKWCASKELKPRTIYIGRPLTEKFPPNEIRNQKYNFFTFLPLVLFEQFRFFLNLYFLIMAVSQFIPEIRIGYLYTYWGPLGFVLAVTICREAVDDLRRHKRDREVNSQKYKRFVSADKPPESVSSSKLRVGDIIMVEKDERVPADLILLRTSDKSGAVFVRTDMLDGETDWKLRLAVPATQKLASHGELLTVNASLYVEKPQRDIHTFIGTYSKHGGTEDEGLNVENTLWANTVVASGTAVGIVIYTGSETRSVMNNSQPRSKVGLLDLEINNLTKVLFCAVIGLSFAMMCLKGFNGPWYRYMFRFVLLFSYIIPISLRVNLDMGKAFYSWQIQNDDEIKGTVVRSTTIPEELGRMSYLLTDKTGTLTQNEMIFKKIHVGTAAYGRDTFPMVSAAIQSVYGTLSAPADASPEDHGSEYQPRLRKPDGWRIWESVKALALCHNVTPVYESNNGNGGSGSISSTSDRRQSPARSISVESQAGEPSTSKPATTEKTYQASSPDEIALVKWTESVGLTLVQRDLNHMTLQIRDATHEARGTSMNENASINTTVTNLSVNSKTDLNSPSSGGSVTSLNSIIAAGAPGLMKYQILQTFPFTSENKRMGIIVRELNGGEITFYLKGADVVMSGIVQYNDWLAEESGNMAREGLRTLVVAKKVLSEEQYNDFVMRYNAAKVSVTDRVAKVAAVIESLEREMELLCLTGVEDRLQDRVRPTLELLRNAGIKIWMLTGDKLETATCIAKSSHLVGRNQSIHVLKSVLTRTDAHLELNQFRRKQDCALVVSGESLEVCLQYYQPEFMELATACPAVVCCRCSPTQKAQVVSLIQKYSKKRTCAVGDGGNDVSMIQQADAGIGIEGREGKQASLAGDFSIPQFSHIARLLIVHGRRSYKRSAALSQFVIHRGLIISTMQAVFSAVFYLSSVALYQGFLMVGYATLYTMFPVFSLVLDQDISANIAITYPELYKELSKGRSLSYKTFFMWVLISIYQGGVIMYGALILFEDEFIHIVAISFSALILTELIMVALTIRTWHKLMVIAELFSLVLYIVSLAVLHEYFDWEFIWSWDFLWKVLVITLVSCLPLYILKFLRKKFSPPSYSKLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VXK8</accession>
    <name>A0A6E8VXK8_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Adenylate kinase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the adenylate kinase family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_040376791.2"/>
    </dbReference>
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    <dbReference type="EnsemblMetazoa" id="ACON009317-RA">
        <property type="protein sequence ID" value="ACON009317-PA"/>
        <property type="gene ID" value="ACON009317"/>
    </dbReference>
    <dbReference type="GeneID" id="120955700"/>
    <dbReference type="CTD" id="203"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005851"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON009317"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037923"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019205">
        <property type="term" value="F:nucleobase-containing compound kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01428">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Adenylat/UMP-CMP_kin"/>
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        <property type="entry name" value="Adenylat_kinase_CS"/>
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        <property type="entry name" value="P-loop_NTPase"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0808">Transferase</keyword>
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    </evidence>
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    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009317-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="198" mass="22067" checksum="B8EF501C23FE7349" modified="2020-08-12" version="1">MMREANVPIIWVLGGPGCGKGTQCAKIVAKYNFSHFSTGDLLRDEVASGSDKGKELQDMMRQGILVPNETVLKLLEAAMVKALNGTVGYLIDGYPREPAQGPEFEKFIAPVDIILYFECSNETLVARIMKRAAESSTVRADDNEETLKTRIATFRENTEKILVQYPTQLRRINAERAPEEIFADVEKSIDELLAAKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VXL5</accession>
    <name>A0A6E8VXL5_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="2 7">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="2 7"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8VXL5"/>
    <dbReference type="EnsemblMetazoa" id="ACON009347-RA">
        <property type="protein sequence ID" value="ACON009347-PA"/>
        <property type="gene ID" value="ACON009347"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001706"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON009347"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037520"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.50.40:FF:000006">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000008">
        <property type="entry name" value="Peptidylprolyl isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.50.40:FF:000013">
        <property type="entry name" value="Peptidylprolyl isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050754">
        <property type="entry name" value="FKBP4/5/8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46512">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46512:SF9">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 7" id="KW-0697">Rotamase</keyword>
    <keyword evidence="4 8" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="PPIase FKBP-type" evidence="10">
        <location>
            <begin position="30"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase FKBP-type" evidence="10">
        <location>
            <begin position="148"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="8">
        <location>
            <begin position="251"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="8">
        <location>
            <begin position="327"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009347-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="398" mass="44145" checksum="0E309FF12D3D239D" modified="2020-08-12" version="1">MEEYHDLSGDGGVQKRILQEGTGDERPSKGCSVSLHYTGTLDADGKKFDSSRDRNEPFQFTLGTGSVIKAFDMGVASMRLGERCILRCAPEYAYGSSGSPPNIPPNATLNFELEILGWKGEDLSPKSDGGIQRFIVQSGSSKKRPTAGGLVKVHLVGRHEGRVFEERDVEFCLDEGKEVGVVAGVELALEKFHKEETARLLLKPQYAFGAQGNSELGVPPNATVEYTVTLTDFEALVERSMMSQDEMLAQAKLLREKGTKYLKEEKHELALKLYNRALTYLYDQSKEGEAAKLAIYLNKILCLQKLNSHDEAKVACVEALKMDSKNVKALYRRGMSNLALGDLDRALQDFSAVLEIEPENKAALNQVTICKHKIKAYNDQQKKVFANMFTKFAQSDSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VZS6</accession>
    <name>A0A6E8VZS6_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Serine/threonine-protein kinase 11-interacting protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VZS6"/>
    <dbReference type="EnsemblMetazoa" id="ACON010218-RA">
        <property type="protein sequence ID" value="ACON010218-PA"/>
        <property type="gene ID" value="ACON010218"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_008689"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON010218"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_029333"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001451">
        <property type="entry name" value="AGAP010218-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000502">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025875">
        <property type="entry name" value="Leu-rich_rpt_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031782">
        <property type="entry name" value="LIP1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15454">
        <property type="entry name" value="NISCHARIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15454:SF69">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE 11-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15904">
        <property type="entry name" value="LIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12799">
        <property type="entry name" value="LRR_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52075">
        <property type="entry name" value="Outer arm dynein light chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="LKB1 serine/threonine kinase interacting protein 1 N-terminal" evidence="6">
        <location>
            <begin position="17"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="761"/>
            <end position="914"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="765"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="826"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="885"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="904"/>
            <end position="914"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF15904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010218-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1230" mass="137458" checksum="72AA30DD2EFA1FC1" modified="2020-08-12" version="1">MNNFRRVRDQKSKMDPTQITALAKLLKESGDKVLNSQFKLTLSGSLLRALNDSFSLIVDKNEMLPPQSFQVVKPNNAKSDVFRDLQFVYDFVQKTVILSLNQFVNDDPYEFAVDISKFRSLRTLEVQKIPINQIVGLLYLRDQLHDLTCMRSISDVTDVLFGCGGDKVADGKSWRSLKSLNISYNMLDYLDGSMEYTPWLEELNLSHNQLVNVSALRSLPNLRVLNVSYNRLNKIPTFHVDAMRKMKVLLLSNNFLEDLEGLACLSCCLNELDLSGNFIVDHGTLLPLSTLVALRSVNLLDNPLACHPKHRHATARYLNKATSETKFVLDNEPLSRYEKTLTGNYDSYKAILVPNAIAGSTLNLSGRNTPASNATTIALDGDYDPPGGSYKSAYDEKDQMNESVVSQKKLRVRRAVISESDNEDTTTRKYSVDASKAINSLKIESSREHLETKKTLEELRNQYGSEWLQSQAGHMVKNVIGYDPGLDKEYESVVHYQRSNITSNKQQTTPIAHTAEVHIEKQHVEEVQRTSTPIGDERSMVQMQPLRESPVGKDFMSESRTTSSEYKSVASNIGDTKYESVDQETISTKGSLNLTDIYRNQEEADEESEVEDNEVLYQVSSRDNGSDFSIVVSDKSIKEKDVVGKTKTRWGIKTLEACERSTSSRITLTFDTMKRDKRERVYQMDAHDCQLLENRLRDMLSQRPLSEMNQRLYKCVVCNAKFSREVNTKRLFDDLQCPECFGGYCIEIKETQLEKETVPTVLGTGDNSKTSVKQTLGSNSDVSNTVPGPSGTHIMDPDMIHNSSSKNSLVHPEVWKSPVKNDQKSGESFNDSSSVSRISQPGSSCDSNQSVAGSTNSERDRDTDLLGNESDIEVLSNPSQSSIEVLDRGYHPSRKASEERRSSGLETINDSSVDSVKQDPLLSVNLQQEDLSVTKQSELNDGIDETLKHTLESSVQESAKDHALAVASSSAAKHHTTHSHKKAFLTNVNLTESSSSGSVTDSVCTAYESIQGVSTEGSALKHAAKGSAIVPVEQNASHDVAGEERKEQNESMTANTASKADSSVLTSVLGGLFQSTNMLMAKNPKGKSDESLLKKFYEPIRYSYNDFANVDHRLKLYLYQNLFEDAQESLKWLVSCVIFDDNRVEEWPSGFDGLFIMSTTKFYIMKKVAAENDDPSTWLKKHLSGTIDRVGIAQSLPWKIGIKFIISAIGGVHVILQDILRTDKLMLFFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W0Z3</accession>
    <name>A0A6E8W0Z3_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nocturnin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CCR4/nocturin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8W0Z3"/>
    <dbReference type="EnsemblMetazoa" id="ACON009893-RC">
        <property type="protein sequence ID" value="ACON009893-PC"/>
        <property type="gene ID" value="ACON009893"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006742"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON009893"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037958"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004535">
        <property type="term" value="F:poly(A)-specific ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007623">
        <property type="term" value="P:circadian rhythm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd09096">
        <property type="entry name" value="Deadenylase_nocturnin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050410">
        <property type="entry name" value="CCR4/nocturin_mRNA_transcr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034965">
        <property type="entry name" value="Deadenylase_nocturnin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121">
        <property type="entry name" value="CARBON CATABOLITE REPRESSOR PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121:SF45">
        <property type="entry name" value="NOCTURNIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="5">
        <location>
            <begin position="130"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="342"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009893-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="476" mass="53479" checksum="6A7DFACD26B23392" modified="2020-08-12" version="1">MLDPCGEIQLTEKPSHGGVPMNGALKKSHLQRVMLQRMGSFTSAPQIKNVDYQDDKLEITDGMSVPDLVDYCRIARGDERPQLVKRKFLKPIDRLNGSDLTDGFKMLQLDSISKTCSEPSATATQLRIFQWNMLSQTLGMHNDGFVRCPLEALTWECRRYQVIEEIVQNDPDIICLQEVDHFKFLQKILSTQNYEGVFFPKPDSPCLYINGNNGPDGCAVFYKKDRLEMVNHFTRVLEVWRVQSNQVAIAAVLRTLDTQQEFCVTTTHLKARKGALLSKLRNEQGKDLLYFIDGVAENRPVILCGDFNAEPIEPIYSTVLNYKPLGLASAYSDLLAEESQDENNQNALNTGVERSGERCSRSSIGSAHSATDDCGLSNGVRTKAEQSAAYEPPYTTWKIREEGEVCHTIDYVFYSKDKLTVKNCLMFPSGEEIGVDRTPSFQYPSDHFSLVCDIELKPASDGSQSDVCSTIPNHQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W1K4</accession>
    <name>A0A6E8W1K4_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Spectrin beta chain</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Probably plays an important role in neuronal membrane skeleton.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the spectrin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040232502.1">
        <property type="nucleotide sequence ID" value="XM_040376568.2"/>
    </dbReference>
    <dbReference type="SMR" id="A0A6E8W1K4"/>
    <dbReference type="EnsemblMetazoa" id="ACON010895-RC">
        <property type="protein sequence ID" value="ACON010895-PC"/>
        <property type="gene ID" value="ACON010895"/>
    </dbReference>
    <dbReference type="GeneID" id="120955574"/>
    <dbReference type="CTD" id="32746"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_013935"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON010895"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033256"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045169">
        <property type="term" value="C:fusome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016328">
        <property type="term" value="C:lateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031594">
        <property type="term" value="C:neuromuscular junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008091">
        <property type="term" value="C:spectrin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045170">
        <property type="term" value="C:spectrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051693">
        <property type="term" value="P:actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016199">
        <property type="term" value="P:axon midline choice point recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042062">
        <property type="term" value="P:long-term strengthening of neuromuscular junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048790">
        <property type="term" value="P:maintenance of presynaptic active zone structure"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007026">
        <property type="term" value="P:negative regulation of microtubule depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007274">
        <property type="term" value="P:neuromuscular synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd21246">
        <property type="entry name" value="CH_SPTB-like_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21248">
        <property type="entry name" value="CH_SPTB_like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10571">
        <property type="entry name" value="PH_beta_spectrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00176">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000020">
        <property type="entry name" value="Spectrin alpha chain, non-erythrocytic 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000003">
        <property type="entry name" value="Spectrin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000004">
        <property type="entry name" value="Spectrin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000011">
        <property type="entry name" value="Spectrin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000018">
        <property type="entry name" value="Spectrin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000019">
        <property type="entry name" value="Spectrin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000028">
        <property type="entry name" value="Spectrin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000033">
        <property type="entry name" value="Spectrin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000059">
        <property type="entry name" value="Spectrin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000083">
        <property type="entry name" value="Spectrin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000106">
        <property type="entry name" value="Spectrin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000024">
        <property type="entry name" value="Spectrin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001589">
        <property type="entry name" value="Actinin_actin-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041681">
        <property type="entry name" value="PH_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001605">
        <property type="entry name" value="PH_dom-spectrin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018159">
        <property type="entry name" value="Spectrin/alpha-actinin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016343">
        <property type="entry name" value="Spectrin_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002017">
        <property type="entry name" value="Spectrin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11915">
        <property type="entry name" value="SPECTRIN/FILAMIN RELATED CYTOSKELETAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15410">
        <property type="entry name" value="PH_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00435">
        <property type="entry name" value="Spectrin"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002297">
        <property type="entry name" value="Spectrin_beta_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00683">
        <property type="entry name" value="SPECTRINPH"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00150">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46966">
        <property type="entry name" value="Spectrin repeat"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00019">
        <property type="entry name" value="ACTININ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00020">
        <property type="entry name" value="ACTININ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3 11" id="KW-0117">Actin capping</keyword>
    <keyword evidence="8 11" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="15">
        <location>
            <begin position="50"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="15">
        <location>
            <begin position="169"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="14">
        <location>
            <begin position="2157"/>
            <end position="2269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="2091"/>
            <end position="2175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="2277"/>
            <end position="2301"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="776"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="988"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="1316"/>
            <end position="1343"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="1628"/>
            <end position="1662"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="1854"/>
            <end position="1881"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="2091"/>
            <end position="2105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="2106"/>
            <end position="2130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="2164"/>
            <end position="2174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00054264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR002297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010895-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="2301" mass="266562" checksum="643EC8FB1F239F4A" modified="2020-08-12" version="1">MTTDISVVRWDPSQGPGNEFIEDIEYDGGNSSSRLFERSRIKALAEERESVQKKTFTKWVNSHLVRVNSPIKDLYVDMRDGKNLIKLLEVLSGERLPRPTKGKMRIHCLENVDKALQFLREQRVHLENIGSHDIVDGNASLNLGLIWTIILRFQIQDITIEETDNKETKSAKDALLLWCQMKTAGYHNVNVRNFTTSWRDGLAFNAIIHKHRPDLIQFDKLSKTNPIQNLNNAFNVAEEKLGLTKLLDAEDIFVDHPDEKSIITYVVTYYHYFSKLKQETVQGKRIGKVVGIAMDNDRMINEYESLTSELLKWIEVTIVQLGDRHFVNSLVGVQQQLAQFSNYRTVEKPPKFVEKGNLEVLLFTLQSKMRANNQKPYTPKEGKMISDINKAWERLEKAEHERELALREELIRQEKLEQLAARFNRKATMRETWLSENQRLVSTDNFGFDLAAVEAAAKKHEAIETDIFAYEERVQAVVAVCNELEAEKYHDIERIAARKDNVLRLWNYLIELLRARRMRLEFSIQLQQNFQEMIYILDSMEEIKQRLLTDDYGKHLMGVEDLLQKHSLVEADINVLGDRVKQVVQNSQKFLVDEEDNYKPCDPSIIVDRVQRLEDAYAELCKLAVERRSRLEENRKLWQFYWDMADEENWIKEKEQIVSADEIGHDLTTVNLLLSKHKALESEIQSHEPQLMAVSEVGDELVRRGHFGADRIDERLKEILAMWSNLRELTEYRRKRLEDAVDYFQLFADADDIDNWMLDALRLVSSEDVGRDEANVQSLLKKHKDVADELKNYAETIEQLHAQAKRLTLNEPEQERVNERLAAIDNRYRELMELAKLRKQRLLDALSLYKLISESDGVEQWIGEKERMLQTMVPGKDIEDVEIMKHRYDGFDKEMNANASRVAVVNQLARQLLHVEHPNSPEIIERQNHLNNSWSKLREQAESKRDDLKSAHGVQTFYIECRETVSWIEDKKRILTETDSLQMDLTGVMTLQRRLSGMERDLAAIQAKLTALENEADAIEGDHPEEAALIRERVATIQTIWEQLTQMLKERDSKLEEAGDLHRFLRDLDHFQAWLTKTQTDVASEDTPTSLPEAEKLLNQHQSIREEIDNYTEDYKKMMDYGEGLTSEPTQTEDPQYMFLRERLKALKDGWEELHQMWENRQVLLSQGLDQQLFNRDARQAEVLLSQQEHVLSKDDTPVNLEQAENQLKRHEAFLTTMEANDEKFNTIVQVAGQMTSKDHFDADKITKRAESIAHRRDDNRNRALELHEKLKNQVKLHEFLQDIEELTEWVQEKYITAQDDTYRSAKTVHSKWTRHQAFEAEIAANKERLHEAKKAAQELMVEKPEFKEIIEPKLTDLSKNFDELETSTKEKGALLFDAKREVIVQQSVDDIDSWMDDLEKQIINTDTGNDLTSVNILMQKQQIIQTQMAVKARQVEELDKQTEVLTKTAPSDVLEPIVEKKTAVNARFEKIKAPLLERQRQLEKKKEAFQFRRDVEDEKLWIDEKMPLAESTEYGNSLFNVHVLKKKHQSLNTEIDNHEPRIMTICNNGQKLIDEGHEDAGSYADLISQLTQKWQELKDAVENRHRQLDQSEKVQQYFFDAAEAESWMSEQELYMMVEDRGKDETSAQNLMKKHESLEQSVEDYADTIRQLGETARQLTTEQHAYSDQVSVKQSQLDKLYAGLKDLAGERRARLDEALQLFMLSREVDDLEQWITDREVVAGSHELGQDYDHITLLWERFNEFAQDTATVGSERVAKANGIADDLIHAGHSDSATIAEWKDGLNESWQDLLELIETRKAMLAASRELHKFFHDCKDVLGRINEKQHGVSEELGRDAGVVSALQRKHQNFIQDLMTLHSQVQQIQEESAKLQAAYAGEKAREITNREHEVLNAWAHLQSMCEERRGKLADTGDLFKFFNMVRTLMLWMEDVVRQMNTSEKPRDVSGVELLMNNHQSLKAEIDTREDNFSACLALGKELLSRNHYASADIKDRLLQLTNSRNALLHRWEERWENLQLILEVYQFARDAAVAEAWLIAQEPYLMSTELGHTIDEVENLIKKHEAFEKSAAAQEERFSALERLTTFELKEMKRRQDAAEEAERQRLEAEAAAARAAAEAEAEAIRQQEAAAARDAADAPGSPHSTREQESDRPSPGGADEGAQEGILTRKHEWESTTKKASNRSWDKVYCVARSGRLTFFKDQRSAKSVPEQTFRGEPPLELKGAQIEIASDYTKKKHVFRIKLSNGGEFLLQCHDDAEMQQWVTALKAQCELDASGEGRSLTLPASSQKDEQKRRSFFTLKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W5F1</accession>
    <name>A0A6E8W5F1_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Thyroid hormone receptor-associated protein complex subunit</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Inhibits cell growth by regulating the Tor pathway upstream of the Tsc1-Tsc2 complex and downstream of Akt1. Acts as a cell death activator during head development.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DDIT4 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040238249.2">
        <property type="nucleotide sequence ID" value="XM_040382315.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8W5F1"/>
    <dbReference type="EnsemblMetazoa" id="ACON011600-RA">
        <property type="protein sequence ID" value="ACON011600-PA"/>
        <property type="gene ID" value="ACON011600"/>
    </dbReference>
    <dbReference type="GeneID" id="120959136"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_003286"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON011600"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030220"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008258">
        <property type="term" value="P:head involution"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045926">
        <property type="term" value="P:negative regulation of growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009968">
        <property type="term" value="P:negative regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032006">
        <property type="term" value="P:regulation of TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.470.40:FF:000003">
        <property type="entry name" value="Charybde, isoform E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.40">
        <property type="entry name" value="RTP801-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012918">
        <property type="entry name" value="RTP801-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038281">
        <property type="entry name" value="RTP801-like_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12478">
        <property type="entry name" value="DNA-DAMAGE-INDUCIBLE TRANSCRIPT 4 PROTEIN DDIT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12478:SF16">
        <property type="entry name" value="PROTEIN CHARYBDE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07809">
        <property type="entry name" value="RTP801_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="23"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="80"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00059352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON011600-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="274" mass="32375" checksum="7AC853BC764CB948" modified="2020-08-12" version="1">MYINFRTPLMPIPANNSNKNSNQSQRHQSQPQQREQQQQQAASQQQQQLPSSQPLRSHHHNNSSSHHHHHHHHHHQQLLQREREAKQQQRELEQQQQQQQLLQQQQQRQQQLQQQQQQQYYSENQYPLEPATIALTASPHEDALQKLTQRLESELRIAKRQHLACTEVLLPADLLPRISAEMFEQSEKEPCGIRGCTIYIEFEDEPDNTRRIATMKTDPNTVSTFELYLTLKQDRRGWTSILPQFLKNLARGSTIMISPEFRLTKNKLYHAYAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W5S4</accession>
    <name>A0A6E8W5S4_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">J domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8W5S4"/>
    <dbReference type="EnsemblMetazoa" id="ACON012194-RB">
        <property type="protein sequence ID" value="ACON012194-PB"/>
        <property type="gene ID" value="ACON012194"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004566"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON012194"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039132"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43948">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43948:SF10">
        <property type="entry name" value="MRJ, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="1">
        <location>
            <begin position="3"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON012194-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="258" mass="29728" checksum="6068B56C25CACA48" modified="2020-08-12" version="1">MVDYYKILDVSRTATEAEIKKAYKKLALRWHPDKNMDNPEESNRRFKEISEAYEVLSDEKKRRIYDQYGKDGLMNNGSDRYHQSTRHRRHNGSSGMDDFEFFGFPFTFRDPEDVFREFFGGSPFDELFRSMSHRDIHILRHGRRANGATNGHHHHHQRHSHPQNVISSPFMTPLMSFSLMDDFFSGAPRGGMSSVSEYTIGGGGPVKRTSTSTTFINGKKLMTKKVYENGTETIMSYENDVLKSKTVNGVAQAIPYNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W646</accession>
    <name>A0A6E8W646_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8W646"/>
    <dbReference type="EnsemblMetazoa" id="ACON012343-RA">
        <property type="protein sequence ID" value="ACON012343-PA"/>
        <property type="gene ID" value="ACON012343"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_013222"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON012343"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032453"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013162">
        <property type="entry name" value="CD80_C2-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23278:SF32">
        <property type="entry name" value="NEUROMUSCULIN, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23278">
        <property type="entry name" value="SIDESTEP PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08205">
        <property type="entry name" value="C2-set_2"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="8"/>
    </dbReference>
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        <property type="match status" value="6"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="7"/>
    </dbReference>
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        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="2 4" id="KW-0472">Membrane</keyword>
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    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026026890" description="Ig-like domain-containing protein" evidence="5">
        <location>
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        </location>
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            <end position="974"/>
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        </location>
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            <end position="935"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1028" mass="114366" checksum="6AD295D5B61E0343" modified="2020-08-12" version="1">MNSIVIVSLWILLHISNTSCQDEKISVLRGEKVELACPIDIATCGELHSLKWFKGTERIAVVSGDGEVAKVEGSYNDRLSLSHEAQTSRLIFRSVEIADEDTYLCETTFLEPSELCENSGAYSVALNVNAVPSVITLQDGNRNSIKNGSHIGPMREGQDLQVVCEVYGARPEPVISWHRSGRVVRDNLVVEEHNGLYTVKSTLSLTLSRQELGATYNCRVEMKDLKLSVDNQFHIDLQVRPTKINLSGVEHHTVQGTKVLLQCQVSGARPAANMTWYNSSKLITEESSEMTSINTKTYLQNDGTFETISQMIFTATRFENGASMRCEADNIVMREDMDRPLHDTLTLEVMYPPVVSVKPENITVNETQDFLIFCDYEANPASLESVKWYRNSQEIKLNSVRYEGGNPEQTALLIKNSTRHDIGAYTCELSNSIGFDVSDNEVYVDVIYKPTVMLTMDPEAPVIENDQANITLLCNIVDGNPMLLTKVRWFLDGEFLKELPECEEETLGTDENLCEVDPSKMLLQNVGREFIGNYSCEGFNAAGWGDVSEENPLDVYYEPGNASMVHYPYIAIKRRSVTFNCSIEDRGNPAATRYRWLRGGKPVLDVVTPSWTVDPVGLDSRTTFSCFAYNEGGEGYPAEVNLEVHAPPAFIQKLQPYTGALFSTANMSLSCRVECIPSCSISWFKDGIGIDESDERYYITNTYLPAEPATGDFESAYSVLHFNMSAWPNGILDRIKDSSNYSCVSTNNSAGPGVRSTTYFGVEYPPENTSLSSTTISVEEGKIPPKIVCSSRAYPEPAFYWTRNGEIIAKGSALIVDDVLERSDAGKYTCTAFNKHGNHSTDAYIDIHYKPTCQIMRKEVEDEDVLICVATGSPRESEFEWSVKYANDSLQSHRIKSDPYESYLTLDDAVSAMRIYVCVASNNVGMGTPCEIEVAGYVAWWLKGDVLTPYILIGAVAALLLAVILVCIIIICICRRKRRQEKFTGESSTAGGSQIEPGEYENLPFHGLQTPPKKANDTITAKSNTYIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W7K3</accession>
    <name>A0A6E8W7K3_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Orcokinin</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8W7K3"/>
    <dbReference type="EnsemblMetazoa" id="ACON012220-RA">
        <property type="protein sequence ID" value="ACON012220-PA"/>
        <property type="gene ID" value="ACON012220"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011832"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON012220"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041031"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026240521" evidence="1">
        <location>
            <begin position="22"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON012220-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="108" mass="11858" checksum="A664898D5FE0AFE5" modified="2020-08-12" version="1">MVKLHLVCGVLLFAFLGWAHGLPVKEQELEEVLTKLQELKRLNDEYLFYNRILLNSAGIYDGSFSAVPRNAVGSPFGRNQRSLDSIGGGNLLKRASLDSIGGGNLLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W9N7</accession>
    <name>A0A6E8W9N7_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Microtubule-actin cross-linking factor 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
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            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON029051-RL">
        <property type="protein sequence ID" value="ACON029051-PL"/>
        <property type="gene ID" value="ACON029051"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007811"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON029051"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040800"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030056">
        <property type="term" value="C:hemidesmosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005882">
        <property type="term" value="C:intermediate filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045104">
        <property type="term" value="P:intermediate filament cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042060">
        <property type="term" value="P:wound healing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21188">
        <property type="entry name" value="CH_PLEC-like_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21189">
        <property type="entry name" value="CH_PLEC-like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
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</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W9Z9</accession>
    <name>A0A6E8W9Z9_ANOCL</name>
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            <fullName evidence="9">DH domain-containing protein</fullName>
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    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
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    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WDB7</accession>
    <name>A0A6E8WDB7_ANOCL</name>
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        <submittedName>
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        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
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            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
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    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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        <property type="gene ID" value="ACON029629"/>
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    <dbReference type="VEuPathDB" id="VectorBase:ACON029629"/>
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        <property type="evidence" value="ECO:0007669"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="Neurexin-related_CASP"/>
    </dbReference>
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        <property type="entry name" value="AXOTACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PIKACHURIN-LIKE PROTEIN"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="4"/>
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        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026114176" evidence="5">
        <location>
            <begin position="28"/>
            <end position="2025"/>
        </location>
    </feature>
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        <location>
            <begin position="1720"/>
            <end position="1741"/>
        </location>
    </feature>
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        <location>
            <begin position="43"/>
            <end position="94"/>
        </location>
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            <begin position="156"/>
            <end position="352"/>
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            <begin position="395"/>
            <end position="576"/>
        </location>
    </feature>
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        <location>
            <begin position="573"/>
            <end position="752"/>
        </location>
    </feature>
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        <location>
            <begin position="754"/>
            <end position="792"/>
        </location>
    </feature>
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        <location>
            <begin position="1000"/>
            <end position="1172"/>
        </location>
    </feature>
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        <location>
            <begin position="1173"/>
            <end position="1210"/>
        </location>
    </feature>
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        <location>
            <begin position="1216"/>
            <end position="1431"/>
        </location>
    </feature>
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        <location>
            <begin position="1432"/>
            <end position="1470"/>
        </location>
    </feature>
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        <location>
            <begin position="1473"/>
            <end position="1667"/>
        </location>
    </feature>
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        <location>
            <begin position="1867"/>
            <end position="1924"/>
        </location>
    </feature>
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        <location>
            <begin position="1981"/>
            <end position="2025"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1905"/>
            <end position="1918"/>
        </location>
    </feature>
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        <location>
            <begin position="1440"/>
            <end position="1457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50025"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON029629-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="2025" mass="227378" checksum="2818158B8A3AEEB3" modified="2020-08-12" version="1">MTYHAASVLLTLCLTLAPIPYDRCVLAIPTNAEPPKEIVYPKCTGPGEPGQCQTFDYRYRFEQTINNCTQFIWGGCGGNLQNNFETYEQCMQQCANETQTPQPPVPLTTTVDPSSTSVRTQTQEAWSLHSSSSTLAPVPDELRGSELTFKETGYEKTFMFAKNNTFIQMDGETIQTFQLRLCREISFQFRTRLPHGLLVYHNVKTPAGVKLDPYALYVIVEKGQLKVVHVFGNHSTSVTVGEGLNRDEWHSVMVRIDVHGARLIARVDNNKEEVYLKGLNHETNYGVSINLMSVVLVGGLSSEEKLHGVKYIIESFVGCIRNVVLSSGKAASDLLPITPLIATKHENVKEGCRNKCHSRQNLCFTGSRCINHYYDISCDCFGTKYEGEHCDIYTATVLTLRGSSYVSYRIYDWKDRVHSPITRISMMFKTNYDDSALFYASGESLKPQYIAASIKNHSAYIEMDFGDGVMSATLGQDLTRHYWHNLTILHEHDKVIAILDDHVRILDLLGPIHNLLFDPEIYFGGGPNLSKRKGLASSNNFVGSLKYVFYNDVSVLFELKKGNPKVHYIGVLQEEFYEANVEVIPITFSYAKSHIWWPMNNPDTLSLKFDFKSSKATAVLAYSEVKTSEGNGFWELRLATDRLSFDLCPDVKSNITHTTYIRIENPTSWHSIQLTYERNDIKFVVDYRHSVTQMYGLSFNIGDKLIIGSSLKAAASGLVGCLRDLKINGVEIEPRYLVKSERVVGDVSLDNCKYVDPCKKPNTCEHGGKCAVKDDGIICDCKDTGYIGKNCHFTKFRKTCEELALLGYSKSDVYSIDIDGNGVFPPARVKCDFQSLANATKTIVEHNLPSQVDVRSATDEDFSFNIQYREFSAEMLQELISHSLYCTQYIKYDCIKAPLELHSSTWFKSSSNNNTVDSLGEVKRGTCPCAIGKNCEDLNHSCNCDANLNRWLSDEGYYKEPPNLGITQMYFLQQKNLDEESQGRVTLGPLECVETNTQKYVVTFTTSQSYIEVPGWRKGDIAFSFRTTGEKAILLFQPPIRSNYPSFMVALTGDYQLTFNFTLNTGTPKTLVIDTNRKLNGGEWHKIWIDYNEHHVRFMINTDTRMVDLQPEEEFGPFEGSMFIGGAPTEISKKYTVKQGLIGCFRGLVVNGEILDIYSFMSVHLSEIIKDCKPSCVPNPCKNGAYCRELWSTFECVCQNRWAHLGQHCETNINEDALTFISRESYLKRNYLSDPEDMRDEREKLRAILNSSLLVNLRTYDRHAFVLYANDHYNNFIHLYLTDQSEMVYLYNYGSDIVNLTIEHSELNNARSIQVAVIRTETNTTLYVNEKNVTVERGFLLLDEYSNKPWTNPELEVLSPHRPPAPPTEYFQFNIGGYDPANLLRPNQDTTELEGYIGCIRGLKIGENLISLREMATQNNAHVTDGVLNGCQMKCDAEPCKNGGICTENFVRQESTCNCEHTSFLGEFCSEEKGASFSGEATLLRRLALADTVGSVRLQLAFSSNDMRRANRVMLLLQSRNDRSYYLMVAITAENHLYIEEDREGATYAARIEGNFMDDARHSVYYTRHGDEASLLVDRAQIPIRAVANKPLVPVPDPGANHVQIGGVNTTDPRFAVYKSYDGCLSNIFIQVNNETMKPLEEYMLFTKSEAETITVINSQGVRSAQCKQFDVIQKLTPFNEPALNMTSVIDKNWVVDAPTRLPYKAVYFDPSTKEEKTQVVFITLTAIFVIIVVCCLIEVYRSDREYRKRIERQTDEDIIWSKEQAAKMQIETSTTNVKGFSYKSIPSDEKKDNGAKPLVGILKNGSVTAASEKPSPVATVAAAPVESLPQSNGTAETGGIRDSQLLDHELQWESLAAEENEALLKSAQHPTEQQSRHGESNGVDGPRARIANGNHHHHHRVTDLDDPSDGHEEDSELTDNPVKVSFAKNTIITNSHPPNARKTPPKLTLTHPPESLLADGPGTPTATTGAIGPLPVAMMTSTPLTNGLRSRGPRQVSWGPPPPPALISANPLPFKIDETPESPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WDM1</accession>
    <name>A0A6E8WDM1_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Peptidase S1 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the peptidase S1 family. CLIP subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8WDM1"/>
    <dbReference type="EnsemblMetazoa" id="ACON029749-RB">
        <property type="protein sequence ID" value="ACON029749-PB"/>
        <property type="gene ID" value="ACON029749"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004944"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON029749"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_029231"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000028">
        <property type="entry name" value="Serine protease easter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051333">
        <property type="entry name" value="CLIP_Serine_Protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24260:SF147">
        <property type="entry name" value="EG:BACR7A4.3 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="3" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="9" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="9" id="KW-0720">Serine protease</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026026254" evidence="10">
        <location>
            <begin position="21"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="11">
        <location>
            <begin position="71"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON029749-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="384" mass="43349" checksum="9D4F2F9476197929" modified="2020-08-12" version="1">MRNIFTHLTVVLTLANIALGQHFLEQPPINYTEGKSFDDCELRFVTSYDQHESERKLQSWVTNIEMDLIVAYGGERAYKGEFQHMAAIGWTRSEATIDYLCGGSLITWRFVLTAAHCSQDHNILPPDTVRLGDTDLASTDDDESAQQIPIARFIKHPQYRESRRYYDIAVVELEKNVIPNSAICVAYVWRELEAPGDLLVAVGFGALGLGRELSPILQKVKLQALDASICAKRIPTNRRQMPEGLRDDQFCARSETMDTCEGDSGGPLQTLRYDLLGNIFSFIVGVVSFGTPCVEGSTGVYTRVSSYLDWIEKEVNQSLSYEVDYGGPVMTKYVDKQFKVHGIVSRLTQGCGEKMIFTSLIPHRLWLESIIFKQLHERLVFSDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WDM5</accession>
    <name>A0A6E8WDM5_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">MAP/microtubule affinity-regulating kinase 3</fullName>
            <ecNumber evidence="5">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Serine/threonine-protein kinase par-1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="27 28">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="27">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Serine/threonine-protein kinase. Involved in the specific phosphorylation of microtubule-associated proteins for MAP2 and MAP4. Phosphorylates the microtubule-associated protein MAPT/TAU. Phosphorylates CDC25C on 'Ser-216'. Regulates localization and activity of some histone deacetylases by mediating phosphorylation of HDAC7, promoting subsequent interaction between HDAC7 and 14-3-3 and export from the nucleus. Regulates localization and activity of MITF by mediating its phosphorylation, promoting subsequent interaction between MITF and 14-3-3 and retention in the cytosol. Negatively regulates the Hippo signaling pathway and antagonizes the phosphorylation of LATS1. Cooperates with DLG5 to inhibit the kinase activity of STK3/MST2 toward LATS1. Phosphorylates PKP2 and KSR1.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="19">Interacts with MAPT/TAU. Interacts with DLG5 (via coiled-coil domain). Interacts with STK3/MST2 and STK4/MST1 in the presence of DLG5. Interacts with YWHAB, YWHAG, YWHAQ and YWHAZ. Interacts with PKP2 (via N-terminus). Interacts with CDC25C. Interacts with KSR1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cell cortex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8WDM5"/>
    <dbReference type="EnsemblMetazoa" id="ACON029469-RA">
        <property type="protein sequence ID" value="ACON029469-PA"/>
        <property type="gene ID" value="ACON029469"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_014020"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON029469"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032840"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034589"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050321">
        <property type="term" value="F:tau-protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12196">
        <property type="entry name" value="MARK1-3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14072">
        <property type="entry name" value="STKc_MARK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14337">
        <property type="entry name" value="UBA_MARK_Par1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001032">
        <property type="entry name" value="KP78b, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000005">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
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    </dbReference>
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        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Kinase associated domain 1, KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="KA1/Ssp2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001772">
        <property type="entry name" value="KA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049508">
        <property type="entry name" value="MARK1-4_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF82">
        <property type="entry name" value="KP78A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02149">
        <property type="entry name" value="KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103243">
        <property type="entry name" value="KA1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50032">
        <property type="entry name" value="KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 22" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 22" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="24">
        <location>
            <begin position="80"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="25">
        <location>
            <begin position="350"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="domain" description="KA1" evidence="26">
        <location>
            <begin position="780"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="417"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="502"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="695"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="834"/>
            <end position="1013"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="41"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="436"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="502"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="522"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="550"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="587"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
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            <begin position="613"/>
            <end position="625"/>
        </location>
    </feature>
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            <begin position="626"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="653"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
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            <begin position="697"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="714"/>
            <end position="725"/>
        </location>
    </feature>
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            <begin position="837"/>
            <end position="850"/>
        </location>
    </feature>
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        <location>
            <begin position="962"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="23">
        <location>
            <begin position="980"/>
            <end position="1005"/>
        </location>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022679"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00054424"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00063680"/>
        </source>
    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00074935"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON029469-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1026" mass="109757" checksum="9102536CFC2B1AC0" modified="2020-08-12" version="1">MSATMRTTLQTVPESLPADHITNSGAPVQCAAQMEPKSHSHSNSAGNKSIRSSTSRGSPNMQMRGTPARWRSGEEHIGKYKLLKTIGKGNFAKVKLAKHVPTNKEVAIKIIDKTQLNPSSLQKLYREVRIMKMLDHPNIVKLFQVIETEKTLYLVMEYASGGEVFDYLVAHGKMKEKEARAKFRQIVSAVQYCHQKRIIHRDLKAENLLLDSEMNIKIADFGFSNEFTPGSKLDTFCGSPPYAAPELFQGRKYDGPEVDVWSLGVILYTLVSGSLPFDGATLKELRERVLRGKYRIPFYMSTDCEVLLKKFLVLNPSKRANLETIMKDKWMNMGYEDDELKPYVEPLPDLKDQKRIEALVAMGYNRQDIEDSLANTMYDDVFATYLLLGRKSTDSESDGSRSGSSLSLRNIAGNEGAAAGNSQVQSPTHRGVHRSISASSTKPSRRASSGGETLRVGPTTAVAAAAAAAAVGAAAGGGAGTGTVVGGGTGGGTANVTGNSVLAGATNNHSSGTGGSGGGASERTSISSNFKRQNTIDSATIKENTARLAAQNQRPASSITKPITSVDNSSISSPAKARTTSSSTSTKYDPSNGSRTVGPSAGLMPRRSTTLYEKTSSTEKTNSTTDPTMVNRNSLTPAVNSSRQPVAFPRNVPSRSTFHSGQTRARNSTVYAGTGGNVGDSPHSGKTFLQRLTTRFSKRDNLRLPSERPNDGQANTSTSTASTNTEEPVKPRVLRFTWSMKTTSPRLPDEIMAEIRSVLDKNNCDYEQRERFVLLCVHGDPNTDSLVQWEIEVCKLPRLSLNGVRFKRISGTSIGFKNIASKIAYDLQYHSVGSDTTQDDGDDGGDDDDGTASGGGGGLTEAESESEQKRSNHHHHARKEGGNGGDGSVEPVEPFVPGGSVRMRSKTPSAAGATGGGSKRWSLVVVDGRSSLRRKEKERISSHHQQSLPRQRKEKMKEEDTGPTTTTTPSRTETVATTSGNGGGGEGEGSGDGGGGGGGGGGSTGTGCQPAGYNSLFASLLRTTTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WE12</accession>
    <name>A0A6E8WE12_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">pantothenate kinase</fullName>
            <ecNumber evidence="4">2.7.1.33</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(R)-pantothenate + ATP = (R)-4'-phosphopantothenate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16373"/>
            <dbReference type="ChEBI" id="CHEBI:10986"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29032"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.33"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 1/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the type II pantothenate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.33" evidence="4"/>
    <dbReference type="RefSeq" id="XP_040236150.1">
        <property type="nucleotide sequence ID" value="XM_040380216.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8WE12"/>
    <dbReference type="EnsemblMetazoa" id="ACON029654-RB">
        <property type="protein sequence ID" value="ACON029654-PB"/>
        <property type="gene ID" value="ACON029654"/>
    </dbReference>
    <dbReference type="GeneID" id="120957829"/>
    <dbReference type="CTD" id="2091"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006932"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON029654"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039494"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004594">
        <property type="term" value="F:pantothenate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd24122">
        <property type="entry name" value="ASKHA_NBD_PanK-II_Pank1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.510:FF:000004">
        <property type="entry name" value="Fbl, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000025">
        <property type="entry name" value="pantothenate kinase 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.510">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004567">
        <property type="entry name" value="Type_II_PanK"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00555">
        <property type="entry name" value="panK_eukar"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12280:SF30">
        <property type="entry name" value="FUMBLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12280">
        <property type="entry name" value="PANTOTHENATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03630">
        <property type="entry name" value="Fumble"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00060870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON029654-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="381" mass="42280" checksum="16BB3E1A4CAAD9A8" modified="2020-08-12" version="1">MASSESAGSSSVIRKAMPWFGMDIGGTLTKLVYFEPKDITPGELDQEARILRNIRRYLTKNSAYGKTGHRDSHLQMDDVVIRGRRGSLHFIRFPTSEMLSFLKLAKSKGMAQLVTTVCATGGGAFKFEEDFRQHVNMKLAKFDELDALIKGILFTETHNKCECYFWENANEISSTKKKFDFSQPYPFILVNVGSGVSVLAVRGPDNYKRISGTSLGGGTFLGLCCLLTGCETFEEAIQLATKGDHKKVDKLVKDIYGGDYERFGLPGELVASSFGQMHLQERRQSVSKEDLAHAILVTITNNIGSIARMCASNEKIEKVVFVGNFLRVNPISMKLLAYAMDYWSKGTLKALFLEHEGYFGAVGCLLQFNGELHAQVGDLLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9I3A7H5</accession>
    <name>A0A9I3A7H5_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Integrin alpha-2 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2023-03" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Membrane</location>
            <topology evidence="1 13">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the integrin alpha chain family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040224340.2">
        <property type="nucleotide sequence ID" value="XM_040368406.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9I3A7H5"/>
    <dbReference type="EnsemblMetazoa" id="ACON000307-RB">
        <property type="protein sequence ID" value="ACON000307-PB"/>
        <property type="gene ID" value="ACON000307"/>
    </dbReference>
    <dbReference type="GeneID" id="120950398"/>
    <dbReference type="CTD" id="32275"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034231"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008305">
        <property type="term" value="C:integrin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033627">
        <property type="term" value="P:cell adhesion mediated by integrin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007157">
        <property type="term" value="P:heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007229">
        <property type="term" value="P:integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.930">
        <property type="entry name" value="Bicelle-embedded integrin alpha(iib) transmembrane segment"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.130">
        <property type="entry name" value="Integrin alpha, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1460">
        <property type="entry name" value="Integrin domains. Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1510">
        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1530">
        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013517">
        <property type="entry name" value="FG-GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013519">
        <property type="entry name" value="Int_alpha_beta-p"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000413">
        <property type="entry name" value="Integrin_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018184">
        <property type="entry name" value="Integrin_alpha_C_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013649">
        <property type="entry name" value="Integrin_alpha_Ig-like_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048285">
        <property type="entry name" value="Integrin_alpha_Ig-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048286">
        <property type="entry name" value="Integrin_alpha_Ig-like_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028994">
        <property type="entry name" value="Integrin_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032695">
        <property type="entry name" value="Integrin_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23220">
        <property type="entry name" value="INTEGRIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23220:SF122">
        <property type="entry name" value="INTEGRIN ALPHA-PS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01839">
        <property type="entry name" value="FG-GAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08441">
        <property type="entry name" value="Integrin_A_Ig_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20805">
        <property type="entry name" value="Integrin_A_Ig_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20806">
        <property type="entry name" value="Integrin_A_Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01185">
        <property type="entry name" value="INTEGRINA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00191">
        <property type="entry name" value="Int_alpha"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69318">
        <property type="entry name" value="Integrin alpha N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69179">
        <property type="entry name" value="Integrin domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51470">
        <property type="entry name" value="FG_GAP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00242">
        <property type="entry name" value="INTEGRIN_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 13" id="KW-0401">Integrin</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 13" id="KW-0732">Signal</keyword>
    <keyword evidence="3 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039960973" evidence="13">
        <location>
            <begin position="25"/>
            <end position="1197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1111"/>
            <end position="1135"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="12">
        <location>
            <begin position="29"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="12">
        <location>
            <begin position="329"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="12">
        <location>
            <begin position="389"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="12">
        <location>
            <begin position="451"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin alpha first immunoglubulin-like" evidence="15">
        <location>
            <begin position="499"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin alpha second immunoglobulin-like" evidence="16">
        <location>
            <begin position="692"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin alpha third immunoglobulin-like" evidence="17">
        <location>
            <begin position="850"/>
            <end position="1100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1150"/>
            <end position="1197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="14">
        <location>
            <begin position="1163"/>
            <end position="1172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF08441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF20805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF20806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000307-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1197" mass="128050" checksum="4EA5F50B15249F07" modified="2023-06-28" version="1">MTHLALAAAAVVVVVVVLAGRTFAFNLETRHMLEKQGQPGSYFGYSVAGHSQRVGGGGGAGGRNGAGNATHWMLVGAPLGQNLQPGSNHSGALFRCPITLAQDDCTQVITDGHRSDDFDDDELLGDGESRLEPPGVDEIKNNQWLGVTVETGGQENKIFVCAHRYIKINNPQSLAYTASGPGSPGLYLGQGVCYVLNEDFSFGFAVEVCRGRSIEREHQQYAFCQSGTSGAVLPDGTALIGTPGAMTWKGTLFNVAIEGGFLSRDKFQYFAPHDNLHSPVPNYSYLGMAVTGGRFFGEYMAYAGGAPRAAQGNGEVVIFTNRQKRNPFDHVVSLRGEQFGSGFGSTLASADINGDGRLDLLVGAPNYFNRTDGGAVYVYLNRGTSFGPEYDQRLTGRLESRFGTAIANCGDLNHDGLDDVAIGAPYESAGSGRVYIHFGTPDGALSPEPVQTIDPSELFATPWRTFGSSLDGGHDLDGNSYPDLIIGSYASDRVVALLARPIINIRTYEEQQAGEPGSIDPTRVGCPSDPTANVTCFSFRACSAVDDASLSEGDVSADSSLQLRCTIQAETFLNKFSRVYFGTGGGPGADKTPAVPGAAATRTNILKRRFRLAVSGRPVCHDVTAYLKEGTRDIQNPIQFRISYTLDEERANGGTGAGGRRGRKERELPRLEPILNRTAAERTYTMPFQKDCGVDGLCQSALVIQNATLVGLARSGGAAGGEYQLVLGLDRTLTLALTVQNRADSAYETHLYVRHDASLTYAGSKGSSVCGAHNATMVDCSIGNPLKRNGEVQLSLRFDARSLEEYGSSKGPNGLSQHVALAVFVNTTSQDTGNRTRAQLNLRIQRAVKLSISGKALPEQVFYGGGGEQIMGESAIHSLDEIGMPVQHKYQIYNDGPWRAPMVRLEIDWPLQVANNKPQGKWLLYLDALPVLDMSGIMGDGRSTCDVLRKDEPDGAVVAGGDSVPLVNPLGLPSRTGPSAPPSPFTALRSANYTRAGRVRRDRAMVLRPQAGDGAVQMDCASQTAKCVRIVCKIHDLGPKSHALVTVTARLWNATLVSDYAQVDVVRIGSSARFVVEGIDELLDQAHQPASLTVRTEAFPEMGPPIVAMDVPWWIILIAAITGLLVLIALTYLLYRCGFFRRKRHTGDDPTLSGNLERRRDPSGGGGGGGGTYDELNDTNGSGGGTAERKPFLGRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9I3AJN3</accession>
    <name>A0A9I3AJN3_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">EamA domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2023-03" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A9I3AJN3"/>
    <dbReference type="EnsemblMetazoa" id="ACON004544-RB">
        <property type="protein sequence ID" value="ACON004544-PB"/>
        <property type="gene ID" value="ACON004544"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON004544-RD">
        <property type="protein sequence ID" value="ACON004544-PD"/>
        <property type="gene ID" value="ACON004544"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041060"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026505">
        <property type="entry name" value="Solute_c_fam_35_mem_F3/F4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19346">
        <property type="entry name" value="SUGAR PHOSPHATE TRANSPORTER DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19346:SF4">
        <property type="entry name" value="SUGAR PHOSPHATE TRANSPORTER DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="237"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="288"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="319"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="344"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="374"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="403"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="438"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="468"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="494"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004544-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="554" mass="59009" checksum="DEA466116A29AB28" modified="2023-06-28" version="1">MVENAMTRDSEIPAIFNPKRVRAPSVVVTGDSPNGPFGGFTNNVPQITHSDSVTSSQHDGQDSLIGITVTGGMHGGCDTPTSQPHALLGSGGGLGVGSGGGSGSANPAVSSSLPSTSRVKACRASCCSELAQKMYFGVCVTVLVTASWVGATHCIKFLYLRRNTYASTLPPHLTAFITDGTTDAIPGGAAPDQNSTSAMIVHGFGPTSSFLPSTTKPIITSDRVAPPLTTHVFNAPFFASWFCTNFAILFFPIYILGRVAIKKCDGTGEVLGEILRGFRDRGFTIGRFLNRCLTFCVLWLLTTYLYALSLKDLLATDVMALFATNVACVYLLAWVILQEQFVGVRIVAVILCDTGIALLAYMDGITGSPTLGGVVLAALAAAGYAVFKVMFRKVMGDPPVGQIAFTFSIIGFLNAAILWPVCIALYFTGAEIMPWETLPWVVLLMASVLLLVFHILTQFSSAVTYNMFVTLGLITAVPVSAALDIVLYGAHFAGMKLAGIILIAVGFFLVMFPDNWPDYITRLLRNIILLGHNASRPAFCRRDIVVASPTSTVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9I3AJR9</accession>
    <name>A0A9I3AJR9_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">EamA domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2023-03" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_040226526.1">
        <property type="nucleotide sequence ID" value="XM_040370592.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9I3AJR9"/>
    <dbReference type="EnsemblMetazoa" id="ACON004544-RC">
        <property type="protein sequence ID" value="ACON004544-PC"/>
        <property type="gene ID" value="ACON004544"/>
    </dbReference>
    <dbReference type="GeneID" id="120951720"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041060"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026505">
        <property type="entry name" value="Solute_c_fam_35_mem_F3/F4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19346">
        <property type="entry name" value="SUGAR PHOSPHATE TRANSPORTER DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19346:SF4">
        <property type="entry name" value="SUGAR PHOSPHATE TRANSPORTER DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="237"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="288"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="319"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="344"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="374"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="403"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="438"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="468"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="494"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004544-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="571" mass="61191" checksum="2DFAD5B47E0C7325" modified="2023-06-28" version="1">MVENAMTRDSEIPAIFNPKRVRAPSVVVTGDSPNGPFGGFTNNVPQITHSDSVTSSQHDGQDSLIGITVTGGMHGGCDTPTSQPHALLGSGGGLGVGSGGGSGSANPAVSSSLPSTSRVKACRASCCSELAQKMYFGVCVTVLVTASWVGATHCIKFLYLRRNTYASTLPPHLTAFITDGTTDAIPGGAAPDQNSTSAMIVHGFGPTSSFLPSTTKPIITSDRVAPPLTTHVFNAPFFASWFCTNFAILFFPIYILGRVAIKKCDGTGEVLGEILRGFRDRGFTIGRFLNRCLTFCVLWLLTTYLYALSLKDLLATDVMALFATNVACVYLLAWVILQEQFVGVRIVAVILCDTGIALLAYMDGITGSPTLGGVVLAALAAAGYAVFKVMFRKVMGDPPVGQIAFTFSIIGFLNAAILWPVCIALYFTGAEIMPWETLPWVVLLMASVLLLVFHILTQFSSAVTYNMFVTLGLITAVPVSAALDIVLYGAHFAGMKLAGIILIAVGFFLVMFPDNWPDYITRLLRNIILLGHNARWGRHPRSGTPSQHRDVIDYRTGYIRSHLRSPSGRVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9I3AK36</accession>
    <name>A0A9I3AK36_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="7">EF-hand domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2023-03" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_040218366.2">
        <property type="nucleotide sequence ID" value="XM_040362432.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9I3AK36"/>
    <dbReference type="EnsemblMetazoa" id="ACON004631-RB">
        <property type="protein sequence ID" value="ACON004631-PB"/>
        <property type="gene ID" value="ACON004631"/>
    </dbReference>
    <dbReference type="GeneID" id="120947261"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_036458"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052110">
        <property type="entry name" value="MCFD2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23104:SF17">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23104">
        <property type="entry name" value="MULTIPLE COAGULATION FACTOR DEFICIENCY PROTEIN 2 NEURAL STEM CELL DERIVED NEURONAL SURVIVAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039945899" evidence="5">
        <location>
            <begin position="22"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="123"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="32"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="46"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004631-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="206" mass="24102" checksum="053E2F4B6D3CADF4" modified="2023-06-28" version="1">MKHSVLITLAILMQLASLAICQRVAPGVNPNHYQQQQQYQPPPPQYQQQAPQHQQPQYQQQPPQQQQQPQYQQQQFQQQQQHQQPHGEHGQQQVLHTGNLQQEKQHIAEHMEVPIDTSKMSEQELQFHYFKMHDSDNNNKLDGCELIKSLIHWHEKDNGEGEHDHEPVAYTDEQLSGIVDAVMKNMDRNNDGVVDWPEYVLSSSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9I3ANZ7</accession>
    <name>A0A9I3ANZ7_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pebble</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2023-03" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A9I3ANZ7"/>
    <dbReference type="EnsemblMetazoa" id="ACON005796-RB">
        <property type="protein sequence ID" value="ACON005796-PB"/>
        <property type="gene ID" value="ACON005796"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040706"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:nervous system development"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000431">
        <property type="term" value="P:regulation of cytokinesis, actomyosin contractile ring assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17732">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01229">
        <property type="entry name" value="PH_Ect2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10190:FF:000063">
        <property type="entry name" value="Pebble, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026817">
        <property type="entry name" value="Ect2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049396">
        <property type="entry name" value="ECT2_BRCT0"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049395">
        <property type="entry name" value="ECT2_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16777">
        <property type="entry name" value="PROTEIN ECT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16777:SF2">
        <property type="entry name" value="PROTEIN ECT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00533">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21243">
        <property type="entry name" value="ECT2_BRCT0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21242">
        <property type="entry name" value="ECT2_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12738">
        <property type="entry name" value="PTCB-BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="107"/>
            <end position="176"/>
        </location>
    </feature>
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            <begin position="197"/>
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            <begin position="394"/>
            <end position="583"/>
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            <begin position="308"/>
            <end position="336"/>
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        <location>
            <begin position="356"/>
            <end position="393"/>
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    </feature>
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        <location>
            <begin position="839"/>
            <end position="879"/>
        </location>
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        <location>
            <begin position="371"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005796-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="879" mass="98567" checksum="88BA6AE29454B29E" modified="2023-06-28" version="1">MSISTSPSTPEMTRICLVGPVAQDAATLAAAQSLKLPIVTSDTGAEYIGDDDISIRTVFVLNDFEGPVYDAIYKAKQRILGPPALQQAVRSSEGLVWNNRPIYNYCMRGVITCFTGIRKKDELTHLVHLIHSMGGSIRKGMDTKVTHLICNSSGGDKYRYAMTFRLAIIRPNWVLEAWKNRHDPNFSATIETFTKQHRLKAFEGQKVCFFGFPEEEQQHMIDVLRTNGGIPTDLEDPECSHVVVDEHVVQAKPETKNKNTHIVKADWFWYTIQAGYANECDYLFADYLESIAHTPVVDRRDSLPVSFNKRKRKRLSQRTPADGGSGGTPLGVGKRRSSVSDAGLLSVSGSFLDCTVSPDKHESSKKNAIPDSAKASADQQQQQQQQQSAKTQSMRHNHFMDFFHTESNYVGILDTIVKLFKEPLEEMVENENRLLNKSELRSIFGNFLPIHDVHKRMLDRLKAIHACWSEEVLIGQIVLDHRDDLLKAYPPYVNFFEQMKETLVQCDATNPRFHAFLKINQAKPECGRQSLQDLMIRPVQRLPSISLLLNDILKHTGKANPDHKKLDDALKAIREVMTYINEDKRKTEGQMAMFDIFNDIDNCPPHLVSSHRSFVSRCEVTELSDSLSGRGDPLVLFLFSDTLEVCKKRSRVFNNVKSPNTNSLNLTRSSCATTKPYKHVKLMPLSTVQLVVNIVDSPRAFMLNCRELAQNAKDRQYSFNIADEEQDKLLYLKSLSKQMAENACRTDAGHFLINSTADEVDIDVSDINVGTLSKAFKIASKTRMKVGRVFSFNKTPSKLKRTVSTMMPSPFGSTQSLTPASQLAQMKLASCTNLNEVCEEGADSNGSSPSSSKLVAPMSVQPTRKNKNPPISIGALRPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9I3AZ20</accession>
    <name>A0A9I3AZ20_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="4">C2 NT-type domain-containing protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2023-03" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the EEIG family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9I3AZ20"/>
    <dbReference type="EnsemblMetazoa" id="ACON009577-RC">
        <property type="protein sequence ID" value="ACON009577-PC"/>
        <property type="gene ID" value="ACON009577"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_029307"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039931">
        <property type="entry name" value="EEIG1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019448">
        <property type="entry name" value="NT-C2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21456:SF1">
        <property type="entry name" value="C2 NT-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21456">
        <property type="entry name" value="FAMILY WITH SEQUENCE SIMILARITY 102"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10358">
        <property type="entry name" value="NT-C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51840">
        <property type="entry name" value="C2_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2 NT-type" evidence="3">
        <location>
            <begin position="12"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="205"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="308"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="373"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="469"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="322"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="484"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="507"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="523"/>
            <end position="543"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00034780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009577-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="621" mass="64588" checksum="D74B5CDC3157DC38" modified="2023-06-28" version="1">MTLIGPGAPGMAFMMKKKKYKFSVELHLEELIEVPFLNAMLFAKVRLLDGGSFQAHSTREEVKNHTVRWGQKFEFPCKMTSNASTGVLDPCTVRISVRKEIKGGRSYQKLGFTDLNLAEFAGSGLTPRKYLLEGYDARHRQDNSMLNVSIRMHMIQGDILFKVPSPSPKSKTALPQDNMALGLQPTDPAMATVGPVVTPVVTRVSTKDDPSAGSVAPGFDSLPKKKPPTQVLPTTIEHQQSFDLDPQSFIITDSGISESSEPPSSLLDFQSNLSLVASLPVSVGAPQQTGSAALPGNQVVPTVGTVPGAIELGHSRNSSNTSQMSKGSGYSSFSHSQHSRQSSEGDSGHQRNICGLHRRLLDGSTRQLTSLTLATPSSSGAGTTSSSGSSAISPSSTGTSVGPGMAAGSSCGSGSGCLPPDCTDAVALPPGGNGLLTDRMAAGAAAAAMASATGMGQAAAGTTGTPLCVASSSSSSGFDTPSMLNNGTNNSSSLNLPSSLALRRPSANINPSSGSIPMSETGSLDRMKSAAERRKKGAGHDGDSGMAPTLSGRVEDTRVNTGLIIDELLKNTKLDHLEDAENPTGLALYISSDGTTMVGSHEVHSRMPAGAFKQVVMETPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABM2A842</accession>
    <name>A0ABM2A842_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_040230300.2">
        <property type="nucleotide sequence ID" value="XM_040374366.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_049464119.1">
        <property type="nucleotide sequence ID" value="XM_049608162.1"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON000043.R236">
        <property type="protein sequence ID" value="ACON000043.P236"/>
        <property type="gene ID" value="ACON000043"/>
    </dbReference>
    <dbReference type="GeneID" id="120953931"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd13990">
        <property type="entry name" value="STKc_TLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22974">
        <property type="entry name" value="MIXED LINEAGE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22974:SF23">
        <property type="entry name" value="TOUSLED-LIKE KINASE, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="1135"/>
            <end position="1414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="20"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="128"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="248"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="383"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
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            <begin position="595"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="772"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="965"/>
            <end position="1015"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1417"/>
            <end position="1489"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="1086"/>
            <end position="1120"/>
        </location>
    </feature>
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        <location>
            <begin position="35"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="63"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="136"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="192"/>
            <end position="204"/>
        </location>
    </feature>
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        <location>
            <begin position="205"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="276"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="396"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="420"/>
            <end position="430"/>
        </location>
    </feature>
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        <location>
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    </feature>
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        </location>
    </feature>
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        <location>
            <begin position="608"/>
            <end position="649"/>
        </location>
    </feature>
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        <location>
            <begin position="650"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="779"/>
            <end position="837"/>
        </location>
    </feature>
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        <location>
            <begin position="996"/>
            <end position="1015"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="1442"/>
            <end position="1468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1469"/>
            <end position="1489"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="1164"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000043.P236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1489" mass="153189" checksum="B2B215A2CAEE0B12" modified="2025-10-08" version="1">MSAGTQLQMAPQTTIVQQVGQVDQTASHHHHTSHHLQQQQQQQQQQQQQSHHHHPQHFNSGNQQQHHQQHQQVATVLSSHQQQQQQTSAAAAAAIAVVLGGGGSGNGGGGGAGGGGGAAAAAAAAAAGSNNGGTATGSSSAKSSSGSLADAAQPTTTTTTTTAGVAVATAGGTTTPALHPPIPVGSGLQQQHHAHHTHHHHHHQQQQQQQQHHQNNNKDQDSNMSTGSSHSDKEQDAIMMSAVAAGLAIGSTPPSEKVSRAASERKRKRKAPDDNGGPGGGGVGAGAGGGGHFRDSRDNIKGLRLTLPPLSDNKKINDYFSKHPGLPAAASSPRVSSSTNSPLVGGITTTITAVGSGGSGSSAASAAAAAAAGNTAGGNNSSALGAISNNSPHVDPGGGGGTTGNTAGSGTGPARKSPNPQYPMYPPSPQTTPLLPVGSNGNNNNNNNNGHNAGGAGAASDLFTTGGSSRTQRQQQQQAAAAAAAAAAAANAAVSSAPSSPSVGNNPASGNNASVLVPPSVSTAMSAAAAAAAAVEAAKQAAATSTPSATTVLSPIITPLVSTAVVSTTGTAGSGVSLQQQQQQYQSGIVLVPSNNSTTSSLMHQKPTAASAGQQKQQTGGGQQQPASAGSTQQRDSQAQTQQSAGGATNNHQLACSASTPGTGTTAPSNCNAAGAAAAADHHHQRVTLVSCNTSSNSSSNGGAAVAVAATGSTTPSGGDLTSSGIGSTNVPLTTTTAATSTTSNNSSIGAGAQNSNSILQLPGGAVVTTTKSIGQKLQQHQATTTSSSSSTNTTGGQQSDSSSNNGGNFGSSKFGQQQQQQPGGASASTGTAPTTPTTLMLTKFVQTDLTQADITERDHDFESSRTRVDELSRLSDEQKCQLSVHQKAIEQHKSQISKCVEVVKKLLKQKSNIEKKEARQKCMQNRLRLGQFVTQRVGATFQENWTDGYAFQELAKRQEEITAEREEIDRQKKLLMKKRPTNSEGGRKRNNSSATGGGAGGTGGAGDRGGTGPGSAGVGTGAVVAMGSGGVVGASAGSVGSGGGGSALHNGNDSTFLKPDPVVSSSSTFTLQEYYECDEILKLRQSALKKEDADLQLEMEKLERERNLHIRELKRIHNEDQSRFNNHPVLNDRYLLLMLLGKGGFSEVHKAFDLKEQRYVACKVHQLNKDWKEDKKANYIKHALREYNIHKALDHPRVVKLYDVFEIDANSFCTVLEYCDGHDLDFYLKQHKTIPEKEARSIIMQVVSALKYLNEIKPPIIHYDLKPGNILLTEGNVCGEIKITDFGLSKVMDEENYNPDHGMDLTSQGAGTYWYLPPECFVVGKNPPKISSKVDVWSVGVIFYQCLYGKKPFGHNQSQATILEENTILKATEVQFANKPTVSNEAKSFIRGCLAYRKEDRMDVFALAKHEYLQPPVSKHNRSSNAQNAHAGGQNSSSTGTGAGGGGGGGGGGGGGSGGSGGAGGNQIGQQTSFSTGMFGNMNQSSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABM2A852</accession>
    <name>A0ABM2A852_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_040230328.2">
        <property type="nucleotide sequence ID" value="XM_040374394.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040230337.2">
        <property type="nucleotide sequence ID" value="XM_040374403.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_049464109.1">
        <property type="nucleotide sequence ID" value="XM_049608152.1"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON000043.R237">
        <property type="protein sequence ID" value="ACON000043.P237"/>
        <property type="gene ID" value="ACON000043"/>
    </dbReference>
    <dbReference type="GeneID" id="120953931"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd13990">
        <property type="entry name" value="STKc_TLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22974">
        <property type="entry name" value="MIXED LINEAGE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22974:SF23">
        <property type="entry name" value="TOUSLED-LIKE KINASE, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="1174"/>
            <end position="1453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="59"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="167"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="287"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="422"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="634"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="811"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1004"/>
            <end position="1054"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1456"/>
            <end position="1528"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="1125"/>
            <end position="1159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="74"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="102"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="175"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="231"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="244"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="315"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="435"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="459"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="479"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="504"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="647"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="689"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="818"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="1035"/>
            <end position="1054"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="1481"/>
            <end position="1507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1508"/>
            <end position="1528"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="1203"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000043.P237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1528" mass="157525" checksum="15259F55279E9881" modified="2025-10-08" version="1">MTDPIRFSLGGAAAIMDHFHTALDPRKQELLEARFLGARMSAGTQLQMAPQTTIVQQVGQVDQTASHHHHTSHHLQQQQQQQQQQQQQSHHHHPQHFNSGNQQQHHQQHQQVATVLSSHQQQQQQTSAAAAAAIAVVLGGGGSGNGGGGGAGGGGGAAAAAAAAAAGSNNGGTATGSSSAKSSSGSLADAAQPTTTTTTTTAGVAVATAGGTTTPALHPPIPVGSGLQQQHHAHHTHHHHHHQQQQQQQQHHQNNNKDQDSNMSTGSSHSDKEQDAIMMSAVAAGLAIGSTPPSEKVSRAASERKRKRKAPDDNGGPGGGGVGAGAGGGGHFRDSRDNIKGLRLTLPPLSDNKKINDYFSKHPGLPAAASSPRVSSSTNSPLVGGITTTITAVGSGGSGSSAASAAAAAAAGNTAGGNNSSALGAISNNSPHVDPGGGGGTTGNTAGSGTGPARKSPNPQYPMYPPSPQTTPLLPVGSNGNNNNNNNNGHNAGGAGAASDLFTTGGSSRTQRQQQQQAAAAAAAAAAAANAAVSSAPSSPSVGNNPASGNNASVLVPPSVSTAMSAAAAAAAAVEAAKQAAATSTPSATTVLSPIITPLVSTAVVSTTGTAGSGVSLQQQQQQYQSGIVLVPSNNSTTSSLMHQKPTAASAGQQKQQTGGGQQQPASAGSTQQRDSQAQTQQSAGGATNNHQLACSASTPGTGTTAPSNCNAAGAAAAADHHHQRVTLVSCNTSSNSSSNGGAAVAVAATGSTTPSGGDLTSSGIGSTNVPLTTTTAATSTTSNNSSIGAGAQNSNSILQLPGGAVVTTTKSIGQKLQQHQATTTSSSSSTNTTGGQQSDSSSNNGGNFGSSKFGQQQQQQPGGASASTGTAPTTPTTLMLTKFVQTDLTQADITERDHDFESSRTRVDELSRLSDEQKCQLSVHQKAIEQHKSQISKCVEVVKKLLKQKSNIEKKEARQKCMQNRLRLGQFVTQRVGATFQENWTDGYAFQELAKRQEEITAEREEIDRQKKLLMKKRPTNSEGGRKRNNSSATGGGAGGTGGAGDRGGTGPGSAGVGTGAVVAMGSGGVVGASAGSVGSGGGGSALHNGNDSTFLKPDPVVSSSSTFTLQEYYECDEILKLRQSALKKEDADLQLEMEKLERERNLHIRELKRIHNEDQSRFNNHPVLNDRYLLLMLLGKGGFSEVHKAFDLKEQRYVACKVHQLNKDWKEDKKANYIKHALREYNIHKALDHPRVVKLYDVFEIDANSFCTVLEYCDGHDLDFYLKQHKTIPEKEARSIIMQVVSALKYLNEIKPPIIHYDLKPGNILLTEGNVCGEIKITDFGLSKVMDEENYNPDHGMDLTSQGAGTYWYLPPECFVVGKNPPKISSKVDVWSVGVIFYQCLYGKKPFGHNQSQATILEENTILKATEVQFANKPTVSNEAKSFIRGCLAYRKEDRMDVFALAKHEYLQPPVSKHNRSSNAQNAHAGGQNSSSTGTGAGGGGGGGGGGGGGSGGSGGAGGNQIGQQTSFSTGMFGNMNQSSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABM2BEY3</accession>
    <name>A0ABM2BEY3_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 16">Guanylate cyclase</fullName>
            <ecNumber evidence="3 16">4.6.1.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 16">
            <text>GTP = 3',5'-cyclic GMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13665"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57746"/>
            <dbReference type="EC" id="4.6.1.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.2" evidence="3 16"/>
    <dbReference type="EnsemblMetazoa" id="ACON030776.R7">
        <property type="protein sequence ID" value="ACON030776.P7"/>
        <property type="gene ID" value="ACON030776"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14042">
        <property type="entry name" value="PK_GC-A_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018297">
        <property type="entry name" value="A/G_cyclase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001170">
        <property type="entry name" value="ANPR/GUC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050401">
        <property type="entry name" value="Cyclic_nucleotide_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11920:SF494">
        <property type="entry name" value="ATRIAL NATRIURETIC PEPTIDE RECEPTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11920">
        <property type="entry name" value="GUANYLYL CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00255">
        <property type="entry name" value="NATPEPTIDER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00452">
        <property type="entry name" value="GUANYLATE_CYCLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14 16" id="KW-0141">cGMP biosynthesis</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="13 15" id="KW-0456">Lyase</keyword>
    <keyword evidence="10 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="32"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="619"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="20">
        <location>
            <begin position="978"/>
            <end position="1108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1218"/>
            <end position="1334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1242"/>
            <end position="1272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1286"/>
            <end position="1312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU003431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON030776.P7"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1334" mass="148438" checksum="8C816E82BAEB1A8F" modified="2025-10-08" version="1">MRSRYHKNALVNMCTRTSRCCWFGGPGWKRVLCYRCVCFLYALIVLGIVLLPAPVESGCRRLVRKPCEAICVRRTPDDVPNAGRRLPIGTNPTRFAAAEGNVSFPLLATATGWTCELRIVVIMPANTSVEASLPRVQPVLEKAEGYIRREGIIPADVAIRWIPFDDRCEQARATVMAMDGTGSDYCGHLILGPSCDFALAPVARIARYIYNDGIPVITGAGYTFDFEEPKTHCENEFHMLIRTGLVSFKRMAFFMIELIRHFNWNRVVYFYERQSYYNVAGPQTGHLLMNTMAEFFRHENITYSPFSTDSARTNLTESLKEKVGLSYAIVIMCASPSTIREIMLAAAELNMVNSGEYVFFNIEIFGSMTATKQPPWYAKNDTDERNQKAKEAFTALLQVVAREPEDEEYRQFSKEVKELTKTKYNHTYAEDEPVSTFVTAFYDAVLLYAYALNDSIAQLGVERALRQPINGTHLAQLMWGRSFKGITGNVTIDSNGDRISNYSLLDLNPETGLFEVVANYYYGGGLQFVEGKAIHWAGDRTKAPPDRPTCGFDGSLCPDNSLPGYAILSLVLGVCVVCMGIASIVGYRHYKLEAEINSMTWKVNPNDVLSCNPSRGHRGSFHSMVKRGSQATIYSEDLNSLPGDRQIYIHFGFYKGCKVAIKKINVQNLSLTRSLMLEFKRMKDIQHDHLVRFYGACLDLHPEPFILTEYCPKGSLQDILENETIKLDWMFKISLMHDIVKGMAFLHSTDLHSHGSLKSSNCVVDSRFVLKVTDFGLHQLRRSTDDADIESYAYWQKLLWTAPELLRDPQCPPAGSQKGDVYSFGIIIQEIVSRQGPFYLGTEEKSPKEIIKLVRDGPGILDAPFRPKVDESSYEDVNNIMIKCWSEEPTDRPDFSGLKTIIRKINKENESGNILDNLLQRMEQYANNLEALVDERTQDYFEEKRKCEELLYQLLPKSVAAQLIMGKSVIAETYDQVTIYFSDIVGFTSISAQSTPMQVVDLLNDLYTCFDSIVENFDVYKVETIGDAYMVVSGLPVRNGNLHAREISRMALRLLAAVYKFTIRHRPNEQLRLRIGLHSGPCVAGVVGLKMPRYCLFGDTVNTASRMESNGEALKIHVSHTTKLLLDTFGTFDLTERGLVPMKGKGEMLTYWLNGERTEAIAAAPLPNGLKVMSPAGTSPASIMASRNGPTLLATDSVPASDPQQQPLLLPTATSASQVNGTVASPPAGILINHHNHHHNNHNNNNNNNNNSPCSINHNHSNSSPNSCHNPSAPAKKLNSVSYSFAKAPGNSSSNNSNGAAAAAAAAASAKASKPKLPEVEALRGASVTKPLLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABM2BEY5</accession>
    <name>A0ABM2BEY5_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">MAGUK p55 subfamily member 6</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MAGUK family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_049464310.1">
        <property type="nucleotide sequence ID" value="XM_049608353.1"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON030713.R19">
        <property type="protein sequence ID" value="ACON030713.P19"/>
        <property type="gene ID" value="ACON030713"/>
    </dbReference>
    <dbReference type="GeneID" id="120956394"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd00071">
        <property type="entry name" value="GMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10832">
        <property type="entry name" value="PDZ_MPP6-MPP2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11862">
        <property type="entry name" value="SH3_MPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008144">
        <property type="entry name" value="Guanylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020590">
        <property type="entry name" value="Guanylate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050716">
        <property type="entry name" value="MAGUK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23122">
        <property type="entry name" value="MEMBRANE-ASSOCIATED GUANYLATE KINASE MAGUK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00072">
        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00856">
        <property type="entry name" value="GUANYLATE_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50052">
        <property type="entry name" value="GUANYLATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="PDZ" evidence="6">
        <location>
            <begin position="8"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="4">
        <location>
            <begin position="114"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate kinase-like" evidence="5">
        <location>
            <begin position="239"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON030713.P19"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="467" mass="53040" checksum="662BF1B96EA24DF7" modified="2025-10-08" version="1">MPGETIKMVGIRRNPDEPLGLTVEVDEHNQLVVARIIAGGMIDRQGLLHPGDVILEVNGVPVTTPEELQGEISVAKESVTLKIGPSVEEEMKSARITMAGGQVKNGRNLDSGKKLTCYMRALFDYDPNEDNLLPCKEIGLTFLRGDILQIINVKDPNWWQAKHAGEDGPTGLIPSQELEERRQAYVPPEADFVHKIGICGTRISKKKRKILYKTKQNSEFDKADLMLYEEVTKMPPFKRKTLVLVGVAGVGRRTLKNRLINSDPDKFGSVLPHTSRQPRPLEESGKAYWFTDREEMEQEIRENKFLEFGEHNGNLYGTHLDSIRDVIRQGKMCVLDCSPAALKTLHNSPEFMPFVLFIAAPGMEQLKLLYSERRSASGSTRNLHFDRQSSIRFSSRRAKTLESLASLYEDDDLISAVEESALLQRKYDKYLDMVIVNEDFDDTFRQVTEALEQLSHEHQWVPVNWIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8UYU1</accession>
    <name>A0A6E8UYU1_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_040225469.1">
        <property type="nucleotide sequence ID" value="XM_040369535.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040225478.1">
        <property type="nucleotide sequence ID" value="XM_040369544.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8UYU1"/>
    <dbReference type="EnsemblMetazoa" id="ACON000675-RA">
        <property type="protein sequence ID" value="ACON000675-PA"/>
        <property type="gene ID" value="ACON000675"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON000675-RB">
        <property type="protein sequence ID" value="ACON000675-PB"/>
        <property type="gene ID" value="ACON000675"/>
    </dbReference>
    <dbReference type="GeneID" id="120951042"/>
    <dbReference type="KEGG" id="acoz:120951042"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012233"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000675"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034182"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009533">
        <property type="entry name" value="FAM107"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16768">
        <property type="entry name" value="DOWN REGULATED IN RENAL CARCINOMA 1/TU3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16768:SF5">
        <property type="entry name" value="FI14214P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06625">
        <property type="entry name" value="DUF1151"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="97"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="48"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="104"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000675-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="136" mass="15070" checksum="71FF8CC9D149E97B" modified="2020-08-12" version="1">MMRPISTSQSVMTDAQGLIVPKKLANPVLESIDRQNLHREMMFNQKIGKSVLNQKSELQRALEKQKERQVLAAQNLAKQTEQTIATELGRVIMQRAQRLEQQKGGSGTGGTDPTGGSINPEYLNARAKLRATVDTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8UYV0</accession>
    <name>A0A6E8UYV0_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8UYV0"/>
    <dbReference type="EnsemblMetazoa" id="ACON000697-RB">
        <property type="protein sequence ID" value="ACON000697-PB"/>
        <property type="gene ID" value="ACON000697"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000592"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000697"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040631"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026082133" evidence="2">
        <location>
            <begin position="20"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="120"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="187"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="124"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="191"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000697-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="258" mass="27439" checksum="7A67264661B3932E" modified="2020-08-12" version="1">MKFLLVTAVICGALALTLGVPVPAAKPDPAPEPAPQPKTDIELMKIPLDGDKELDVITLVNSEDQKINERNKRTIGILRELFPTISQITTNENANTLEGLDLAGLSDTLNAIRVARATGEEKAASSNDQNAAESSANSSSLDELTLDSEGNDEDRNKRFLSFGGSSGGGGSGNFLFDIIRLVSGSSGTEQSDEKAHSPDDHDLGKGDGYTEGIPGPVTRLFVLANRGLSNLIQDLILRIAQTSERVVNFKARLITSLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8UYV6</accession>
    <name>A0A6E8UYV6_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Otopetrin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the otopetrin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8UYV6"/>
    <dbReference type="EnsemblMetazoa" id="ACON000703-RC">
        <property type="protein sequence ID" value="ACON000703-PC"/>
        <property type="gene ID" value="ACON000703"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002138"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000703"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033209"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015252">
        <property type="term" value="F:proton channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004878">
        <property type="entry name" value="Otopetrin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21522:SF62">
        <property type="entry name" value="OTOPETRIN-LIKE A, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21522">
        <property type="entry name" value="PROTON CHANNEL OTOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03189">
        <property type="entry name" value="Otopetrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="10" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="73"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="110"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="204"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="243"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="273"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="559"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="627"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="653"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="699"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="730"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="772"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="801"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="138"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="395"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="144"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="156"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000703-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="844" mass="92356" checksum="FE571BDBDB4ED419" modified="2020-08-12" version="1">MVGGGEMKVATVDVESNDNMATLPVSRGHGLGSGAPAPVGGGGSSDTAEKNNAANKEMELKNVMPKPLQRTSLFIVTSLVYAMLLIVVCVAYVISDVTTHRLPVIYYEGFFTYLYGASILFLLYVFCFLLQESSCCNGKPKPPKEKKPKKEKKSKKNADVEAGKEGKDGKEAGAAKDAAQPNPEAAMSPRFKRKTTQDPAHGSFFLRVGAIAFGLGTMIYTGLEFGSFFEIPFTSPCHQILRGVNPLLQMIFTFMQMYFIFMNARLNIHRFKVLARFGLMHIVATNICVWIRTLVLESLKEITAYHQRRGPEPEDSVILENIRQHTLRNAGMVMGMELAPGGTDTEWEPISVNLNAQEDLLSQDASSVLSKIVQGTAHTITEAATTLVSAATLTSTAAPTTTSSSTSTSTSTTTTTTTTTASPTTTPSWIEPSTGASSTTTARGLLDRIRDIVATETTTTYETPANHFGSAQHTLSTSAGGVNATAATPSTGSSFLDSFMDHVNYLQRNSSLDQTYESLDALFPSAFIATSTAVSTNATAISCGRVNIMGTIVQDSAPYLYPFIIEYSLIGAAVIYVMWKHIGRYPKFTNEEDLEHRLEVMLSRRAVVMAQQARTGRVDCVGASKGLFFGLLLLVGSLICLILFFVLVRHPQLSLLAIYLADVSHCALMALAIFAIIIGFIRVQNLKFRCEEQSNLNDILLRISAFGLFVYSTFSVIAGSLNAFESEPNLLVMVTGIVAVVQVVIQLLFIADVSRRRVHLPEHDRIKPGRQIVTFLLICNISMFAIYTFEAQKVFANPVQLDFYGFIAWSLIQRVTLPLCIFHRFHSAVTLAEVWKTTYKARLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V2B1</accession>
    <name>A0A6E8V2B1_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Fructose-bisphosphate aldolase</fullName>
            <ecNumber evidence="3 7">4.1.2.13</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>beta-D-fructose 1,6-bisphosphate = D-glyceraldehyde 3-phosphate + dihydroxyacetone phosphate</text>
            <dbReference type="Rhea" id="RHEA:14729"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.1.2.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the class I fructose-bisphosphate aldolase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.13" evidence="3 7"/>
    <dbReference type="RefSeq" id="XP_040230339.1">
        <property type="nucleotide sequence ID" value="XM_040374405.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8V2B1"/>
    <dbReference type="EnsemblMetazoa" id="ACON002564-RA">
        <property type="protein sequence ID" value="ACON002564-PA"/>
        <property type="gene ID" value="ACON002564"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON002564-RB">
        <property type="protein sequence ID" value="ACON002564-PB"/>
        <property type="gene ID" value="ACON002564"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON002564-RC">
        <property type="protein sequence ID" value="ACON002564-PC"/>
        <property type="gene ID" value="ACON002564"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON002564-RD">
        <property type="protein sequence ID" value="ACON002564-PD"/>
        <property type="gene ID" value="ACON002564"/>
    </dbReference>
    <dbReference type="GeneID" id="120953950"/>
    <dbReference type="CTD" id="43183"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005805"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002564"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031152"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00183"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004332">
        <property type="term" value="F:fructose-bisphosphate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00948">
        <property type="entry name" value="FBP_aldolase_I_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000021">
        <property type="entry name" value="Fructose-bisphosphate aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029768">
        <property type="entry name" value="Aldolase_I_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
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        <property type="entry name" value="FrucBisAld_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11627">
        <property type="entry name" value="FRUCTOSE-BISPHOSPHATE ALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Glycolytic"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ALDOLASE_CLASS_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0324">Glycolysis</keyword>
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    <keyword evidence="6" id="KW-0704">Schiff base</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010387"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00013068"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002564-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="363" mass="39190" checksum="A70F3ED1F5B82099" modified="2020-08-12" version="1">MTTYFNYPPKEIQEELARIAKQIVAPGKGILAADESTATCGKRFADIGVENNEDNRRQYRQLLFTADQRLQEHISGVILFHETLYQKGDDGTPLAKLLASKGILAGIKVDKGVVDLMGSEGECTTQGLDDLAARCAQYKKDGCDFAKWRCVLKIGKNTPSYQSILENANVLARYASICQSQRIVPIVEPEILPDGDHDLERCQKVTETVLAAVYKALNDHHVYLEGTLLKPNMVTAGQSCAKKPTAQEIALATVTALRRTVPAAVPGVTFLSGGQSEEEASVNLSAINQVPLLRPWALTFSYGRALQASVLRAWGGKKENVKAAQEELIKRAKANSDAALGKYGGGVEGAAGAGSLFVANHAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V4K6</accession>
    <name>A0A6E8V4K6_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8V4K6"/>
    <dbReference type="EnsemblMetazoa" id="ACON002010-RA">
        <property type="protein sequence ID" value="ACON002010-PA"/>
        <property type="gene ID" value="ACON002010"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012003"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002010"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_029588"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002010-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="78" mass="8851" checksum="5A3E7FD80CFD80E7" modified="2020-08-12" version="1">MCLDQTEARRKILRGRRTINRTFKRGPMIPAWAIITIVAIVNLLLGGIAYLIFRKVVLNAPIENVTSYTPAMQDEYSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V8Q2</accession>
    <name>A0A6E8V8Q2_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Flavodoxin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8V8Q2"/>
    <dbReference type="EnsemblMetazoa" id="ACON000500-RA">
        <property type="protein sequence ID" value="ACON000500-PA"/>
        <property type="gene ID" value="ACON000500"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004146"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000500"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042582"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003958">
        <property type="term" value="F:NADPH-hemoprotein reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009725">
        <property type="term" value="P:response to hormone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001094">
        <property type="entry name" value="Flavdoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19384:SF17">
        <property type="entry name" value="NADPH--CYTOCHROME P450 REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19384">
        <property type="entry name" value="NITRIC OXIDE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00258">
        <property type="entry name" value="Flavodoxin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00369">
        <property type="entry name" value="FLAVODOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="Flavodoxin-like" evidence="3">
        <location>
            <begin position="83"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000500-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="180" mass="19907" checksum="E97E8FFFBE706160" modified="2020-08-12" version="1">MDAQTETEVPAGSVSDEPFLGPLDIVLLVSLLAGTAWYLLKGKKKESQASQFKSYSIQPTTVNTMTMVENSFIKKLQSSGRRLVVFYGSQTGTAEEFAGRLAKEGIRYQMKGMVADPEECNMEELLMLKDIDKSLAVFCLATYGEGDPTDNCMEFYDWIQNNDLDMTGLNYAVSVVTGHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V8Q6</accession>
    <name>A0A6E8V8Q6_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="10">TEA domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8V8Q6"/>
    <dbReference type="EnsemblMetazoa" id="ACON000512-RB">
        <property type="protein sequence ID" value="ACON000512-PB"/>
        <property type="gene ID" value="ACON000512"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000895"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000512"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034347"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048568">
        <property type="term" value="P:embryonic organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035329">
        <property type="term" value="P:hippo signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.50.80:FF:000001">
        <property type="entry name" value="Transcriptional enhancer factor TEF-1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.50.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.20.40">
        <property type="entry name" value="TEA/ATTS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000818">
        <property type="entry name" value="TEA/ATTS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038096">
        <property type="entry name" value="TEA/ATTS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050937">
        <property type="entry name" value="TEC1_TEAD_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016361">
        <property type="entry name" value="TEF_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041086">
        <property type="entry name" value="YBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11834:SF0">
        <property type="entry name" value="PROTEIN SCALLOPED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11834">
        <property type="entry name" value="TRANSCRIPTIONAL ENHANCER FACTOR TEF RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01285">
        <property type="entry name" value="TEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17725">
        <property type="entry name" value="YBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002603">
        <property type="entry name" value="TEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00065">
        <property type="entry name" value="TEADOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00426">
        <property type="entry name" value="TEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00554">
        <property type="entry name" value="TEA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51088">
        <property type="entry name" value="TEA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TEA" evidence="9">
        <location>
            <begin position="40"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="TEA" evidence="7">
        <location>
            <begin position="40"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR002603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000512-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="381" mass="43020" checksum="AD22BD04E419F29E" modified="2020-08-12" version="1">MGPGPTAGSLVPDDTNGSGVDGGKHLDVGDMSDDEQDLSSADAEGVWSPDIEQSFQEALAIYPPCGRRKIILSEEGKMYGRNELIARYIKLRTGKTRTRKQVSSHIQVLARRKLREFQAKMKVQFWQPGLQPSTSQDVKPFAQPGYPMKTATAVSEGALQPAHPWEGRAIATHKFRLVEYSAYLELRAEETYHKHLFVHIADTPANPLLESVEVKEIYDKFPQKSGGLKELYEKGPSNAFFLVKFWADLNTNIANDAGAFYGVSSHYESNDNMVITCSTKVCSFGKQVVEKVETEYSRIENGRYVYRISRSPMCDYMINFINKLKHLPEKYMMNSVLENFTILQVISNKDTDETLLCVAFVFEVSTSEHGAQHHIYRLVKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V8R1</accession>
    <name>A0A6E8V8R1_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">Glutathione synthetase</fullName>
            <shortName evidence="12">GSH-S</shortName>
            <ecNumber evidence="3 12">6.3.2.3</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>gamma-L-glutamyl-L-cysteine + glycine + ATP = glutathione + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13557"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58173"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:13558"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12 14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="12 14">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 12">Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the eukaryotic GSH synthase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.3" evidence="3 12"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8V8R1"/>
    <dbReference type="EnsemblMetazoa" id="ACON000534-RB">
        <property type="protein sequence ID" value="ACON000534-PB"/>
        <property type="gene ID" value="ACON000534"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012490"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000534"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_029170"/>
    <dbReference type="UniPathway" id="UPA00142">
        <property type="reaction ID" value="UER00210"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043295">
        <property type="term" value="F:glutathione binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004363">
        <property type="term" value="F:glutathione synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1080.10:FF:000011">
        <property type="entry name" value="Glutathione synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.50:FF:000002">
        <property type="entry name" value="Glutathione synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.80:FF:000009">
        <property type="entry name" value="Glutathione synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1760:FF:000007">
        <property type="entry name" value="Glutathione synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1760">
        <property type="entry name" value="Glutathione synthase, substrate-binding domain superfamily, eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1080.10">
        <property type="entry name" value="Glutathione Synthetase, Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005615">
        <property type="entry name" value="Glutathione_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014042">
        <property type="entry name" value="Glutathione_synthase_a-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014709">
        <property type="entry name" value="Glutathione_synthase_C_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014049">
        <property type="entry name" value="Glutathione_synthase_N_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037013">
        <property type="entry name" value="GSH-S_sub-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004887">
        <property type="entry name" value="GSH_synth_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01986">
        <property type="entry name" value="glut_syn_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11130">
        <property type="entry name" value="GLUTATHIONE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11130:SF0">
        <property type="entry name" value="GLUTATHIONE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03917">
        <property type="entry name" value="GSH_synth_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03199">
        <property type="entry name" value="GSH_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001558">
        <property type="entry name" value="GSHase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 12" id="KW-0317">Glutathione biosynthesis</keyword>
    <keyword evidence="5 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="10 12 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 12 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutathione synthase substrate-binding" evidence="15">
        <location>
            <begin position="206"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="309"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="368"/>
            <end position="377"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="372"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="379"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="401"/>
            <end position="404"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="430"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="455"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="457"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="463"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR001558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR001558-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR001558-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF03199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000534-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="480" mass="52818" checksum="485F734C7F25443D" modified="2020-08-12" version="1">MCTPSKTLQSCVPFPLDDALLQEVVEKAKDWAIMHGAAMRSKQDFNPDALLFAPFILTPSSFPRKEFQKAVELQPLLNELIHAVAHDSQFLLATLSSTEKVDEFTRALIAIYRTVLDEGIAQTVTLALLRSDYLPNFSDTIKQVEINTIASSFGGISTIMTPMHSYILSELGHREKLVNLPDNKALSGLCDAMVEAWKLQARPSAAILFVVENVTYNICDQRFHEFYIRETYPHVAVVRRTLTQIVEQGRLGPGKELLVGDVEASVVYFRAGYEPGHYFGAGEWDARLLIERSVAIKCPSIQYHLAGTKKVQQALAKPDVLRRFVGDDAPKIEAIRDIFTGLYSLDREEGGDEAVALALADPERYVLKPQREGGGNNVYGADIPAALQAMSSAERSAWILMERIFPPLSTGYMVRPNGPVPPPLVQLVSELGIFGAIIGSKDKVLYNKQIGHMLRTKLSSSNEGGVAAGSGALDSPYLID</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V8S4</accession>
    <name>A0A6E8V8S4_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Moesin/ezrin/radixin homolog 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Involved in connections of major cytoskeletal structures to the plasma membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Interacts with cytoskeletal actin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell junction</location>
            <location evidence="4">Adherens junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Microvillus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Cell projection</location>
            <location evidence="14">Rhabdomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8V8S4"/>
    <dbReference type="EnsemblMetazoa" id="ACON000562-RA">
        <property type="protein sequence ID" value="ACON000562-PA"/>
        <property type="gene ID" value="ACON000562"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006774"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000562"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041662"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098592">
        <property type="term" value="C:cytoplasmic side of apical plasma membrane"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005902">
        <property type="term" value="C:microvillus"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0016028">
        <property type="term" value="C:rhabdomere"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:anatomical structure formation involved in morphogenesis"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:compound eye photoreceptor development"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:epithelial structure maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:pole plasm assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0042127">
        <property type="term" value="P:regulation of cell population proliferation"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0050793">
        <property type="term" value="P:regulation of developmental process"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0006403">
        <property type="term" value="P:RNA localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13194">
        <property type="entry name" value="FERM_C_ERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17187">
        <property type="entry name" value="FERM_F1_ERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000003">
        <property type="entry name" value="Radixin isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.10:FF:000002">
        <property type="entry name" value="radixin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000013">
        <property type="entry name" value="radixin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.450:FF:000001">
        <property type="entry name" value="radixin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.360.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011174">
        <property type="entry name" value="ERM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011259">
        <property type="entry name" value="ERM_C_dom"/>
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    <dbReference type="InterPro" id="IPR041789">
        <property type="entry name" value="ERM_FERM_C"/>
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        <property type="entry name" value="ERM_helical"/>
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    <dbReference type="InterPro" id="IPR000798">
        <property type="entry name" value="Ez/rad/moesin-like"/>
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    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
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    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
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        <property type="entry name" value="FERM_CS"/>
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        <property type="entry name" value="FERM_domain"/>
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        <property type="entry name" value="FERM_N"/>
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        <property type="entry name" value="FERM_PH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008954">
        <property type="entry name" value="Moesin_tail_sf"/>
    </dbReference>
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        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23281">
        <property type="entry name" value="MERLIN/MOESIN/EZRIN/RADIXIN"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="BAND41"/>
    </dbReference>
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        <property type="entry name" value="ERMFAMILY"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Second domain of FERM"/>
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    <keyword evidence="11" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="9" id="KW-0965">Cell junction</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="18" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="5"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="449"/>
            <end position="516"/>
        </location>
    </feature>
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        <location>
            <begin position="302"/>
            <end position="436"/>
        </location>
    </feature>
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        <location>
            <begin position="456"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="474"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="492"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <begin position="60"/>
            <end position="63"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol)</name>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="278"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol)</name>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
        </ligand>
    </feature>
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            <dbReference type="ARBA" id="ARBA00004105"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022025"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
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        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
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    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00057575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00063162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR002305-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000562-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="578" mass="68319" checksum="9C529522CE438239" modified="2020-08-12" version="1">MPKSMNVRVTTMDAELEFAIQQGTTGKQLFDQVVKTIGLREVWFFGLQYTDSKGDNTWIKLYKKVMSQDVQKGDPLLFKFRAKFYPEDVAEELIQDITLRLFYLQVKNAILSDEIYCPPETSVLLASYAVQARHGDYNKGTHVPGFLAKDRLLPQRVIDQHKMSKDEWENSITTWWQEHRGLLREDAMMEYLKIAQDLEMYGVNYFEIRNKKGTELWLGVDALGLNIYEKEDRLTPKIGFPWSEIRNISFNDRKFIIKPIDKKAPDFVFFAPRVRINKRILALCMGNHELYMRRRKPDTIDVQQMKAQARDEKNAKQQEREKLQLALAARERAEKKQQEYEDRLRSMQEEMERKQANLSEAQDTIRRLQEQLNQVQAAKEELEQRQNELHEMMQRLEETKNMEATERAKLEEEIRVKQLEMQKIQEEVTLKDSETKRLHEEVEEAIRKQVAKGSRAAAALQAATTTPKHHHVEEEEENEEELINGENGTQDFSKDFDTDEHIKDPVEERRTLAERNERLQDQLKALKQDLALSRDDTMETPNDKIHRENVRQGRDKYKTLREIRKGNTKRRVDQFENM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V8T1</accession>
    <name>A0A6E8V8T1_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">thioredoxin-disulfide reductase (NADPH)</fullName>
            <ecNumber evidence="3">1.8.1.9</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Thioredoxin system is a major player in glutathione metabolism, due to the demonstrated absence of a glutathione reductase. Functionally interacts with the Sod/Cat reactive oxidation species (ROS) defense system and thereby has a role in preadult development and life span. Lack of a glutathione reductase suggests antioxidant defense in Drosophila, and probably in related insects, differs fundamentally from that in other organisms.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>[thioredoxin]-dithiol + NADP(+) = [thioredoxin]-disulfide + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20345"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.8.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="14">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.9" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8V8T1"/>
    <dbReference type="EnsemblMetazoa" id="ACON000565-RC">
        <property type="protein sequence ID" value="ACON000565-PC"/>
        <property type="gene ID" value="ACON000565"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_013156"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000565"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032148"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004362">
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    <sequence length="505" mass="54658" checksum="52690F7893185C02" modified="2020-08-12" version="1">MAAATAAEQENYEYDLVVIGGGSGGLACAKQAVQLGAKVAVLDFVKPSPRGTKWGLGGTCVNVGCIPKKLMHQASLLGEAIHDSQPYGWQLPDPAAIRHDWATLTESVQNHIKSVNWVTRVDLRDQKVEYVNGLGYFKDDHTVVAVMKNQTERELRAKHVVIAVGGRPRYPDIPGAAEYGITSDDIFSLPQAPGRTLLVGAGYIGLECAGFLKGLGYDVSVMVRSILLRGFDQQMATMVGDSMVEKGIRFHHRSRPLAVEKQPDGRLLVRYETVDEAGTATNGEDVFDTVLFAIGRQAETSTLKLANAGVVTAEGGKSDKLEVDETDHRTNVPHIYAVGDVLYRKPELTPVAIHAGRIIARRLFGGSEERMDYADVATTVFTPLEYGCVGLSEEAAEAAHGKDGIEVYHAYYKPTEFFVPQRSVRYCYLKAVALREGNQRVLGLHFLGPAAGEVIQGFAAALKCGLTMQVLRNTVGIHPTVAEEFTRLAITKSSGLDPTPATCCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V8T3</accession>
    <name>A0A6E8V8T3_ANOCL</name>
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        <submittedName>
            <fullName evidence="13">Fork-head domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8V8T3"/>
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        <property type="protein sequence ID" value="ACON000569-PB"/>
        <property type="gene ID" value="ACON000569"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
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    <dbReference type="GO" id="GO:0005634">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Forkhead box P2 isoform B"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001766">
        <property type="entry name" value="Fork_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050998">
        <property type="entry name" value="FOXP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032354">
        <property type="entry name" value="FOXP-CC"/>
    </dbReference>
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        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
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        <property type="entry name" value="WH_DNA-bd_sf"/>
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    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45796">
        <property type="entry name" value="FORKHEAD BOX P, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45796:SF4">
        <property type="entry name" value="FORKHEAD BOX P, ISOFORM C"/>
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    </dbReference>
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        <property type="entry name" value="Forkhead"/>
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        <property type="entry name" value="FOXP-CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00053">
        <property type="entry name" value="FORKHEAD"/>
    </dbReference>
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        <property type="entry name" value="FH"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50039">
        <property type="entry name" value="FORK_HEAD_3"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Fork-head" evidence="12">
        <location>
            <begin position="392"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Fork-head" evidence="10">
        <location>
            <begin position="392"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="215"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="289"/>
            <end position="374"/>
        </location>
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        <location>
            <begin position="479"/>
            <end position="585"/>
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        <location>
            <begin position="220"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="238"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="306"/>
            <end position="315"/>
        </location>
    </feature>
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        <location>
            <begin position="316"/>
            <end position="325"/>
        </location>
    </feature>
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        <location>
            <begin position="326"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="341"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="531"/>
            <end position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50039"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000569-PB"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="585" mass="63875" checksum="6594120CB63A0595" modified="2020-08-12" version="1">VCVCVRAGNAQQECHFRLDTPSRPLQLVQFDARAEICRHDQHVLKFHFFAAIPTWRTCGLAPRTRFEPSEQRALRLLAVLPSAPPSKQIAFLSFFTNTPPCGTFRFADEPGNGSSSSRAGSTTIHPLFGGGACRWPGCERVFDEFDDFYSHLHSDHINGDYSAAQARIQMEVVCQLHLQLQKERDRLQAMILHLNKKNEMLRATPAAALFGGSFLSQLPAIPPPPPPPPPPPAPSTQPPSSGSSGAQPGGSVTPVGPFDLIAGLRFPAELAADMVQGAFGGVRLTHPQHHVGLGGAAHHLHHHHGAQQQQQQQQPHPHHHHHHQPALHSLSPPGAGSLGAATGGNGGTKSSKGGGAVRPKYFSPQDSDELNYPDVQDDVHKNREFYRSHDVRPPFTYASLIRQAIIESPEKQLTLNEIYNWFQNTFCYFRRNAATWKNAIRTNLSLHKCFVRYEDDFGSFWMVDDHEFLKRRHLSRGRPRKYDISMPPLADPPAAGAGGPLPTPAGRPSGPASEQGDRAGSLHSPADTLGPDGGGAGGYGEEGEYGREPTGGPTGSRSVPEKELQHGGGRQRHQPATKKPDYSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V8T7</accession>
    <name>A0A6E8V8T7_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">AMP deaminase</fullName>
            <ecNumber evidence="9">3.5.4.6</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">AMP deaminase plays a critical role in energy metabolism. Catalyzes the deamination of AMP to IMP and plays an important role in the purine nucleotide cycle.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>AMP + H2O + H(+) = IMP + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:14777"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="3.5.4.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; IMP biosynthesis via salvage pathway; IMP from AMP: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the metallo-dependent hydrolases superfamily. Adenosine and AMP deaminases family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.6" evidence="9"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8V8T7"/>
    <dbReference type="EnsemblMetazoa" id="ACON000577-RB">
        <property type="protein sequence ID" value="ACON000577-PB"/>
        <property type="gene ID" value="ACON000577"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_003237"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000577"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040059"/>
    <dbReference type="UniPathway" id="UPA00591">
        <property type="reaction ID" value="UER00663"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003876">
        <property type="term" value="F:AMP deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046033">
        <property type="term" value="P:AMP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032264">
        <property type="term" value="P:IMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01319">
        <property type="entry name" value="AMPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.800.20:FF:000001">
        <property type="entry name" value="AMP deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000035">
        <property type="entry name" value="Probable amp deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.800.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006650">
        <property type="entry name" value="A/AMP_deam_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006329">
        <property type="entry name" value="AMPD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01429">
        <property type="entry name" value="AMP_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11359">
        <property type="entry name" value="AMP DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11359:SF0">
        <property type="entry name" value="AMP DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19326">
        <property type="entry name" value="AMP_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001251">
        <property type="entry name" value="AMP_deaminase_met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00485">
        <property type="entry name" value="A_DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="109"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00054146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR001251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000577-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="686" mass="79384" checksum="0097E8FAEB0ADCA2" modified="2020-08-12" version="1">MLGSASDTKKWLSERDLLRVMEQDRHSIAPEVHPDEQLPFDEHDFVAHFQRVSISGEDTSGVPLDDLERASTLLVKALELREKYMRNSHQAFPQTTARFLKSTHSSTITHQERKSIADHPVNPPQSTQSPWVVEIPEDLNYSIRPVHGVFEVFPTVESTEPLPYQYTRLPDFVQDMQMMCSMIADGPLKSFCYRRLSYLYSKFQLHVLLNELRELASQKAVPHRDFYNIRKVDTHIHAASCMNQKHLLRFIKKTLKNSADEVVTVTKGTPMTLAQVFQSMNLTTYDLTVDMLDVHADRNTFHRFDKFNAKYNPIGESRLREVFLKTDNYLNGKYFANIIKEVASDFEESKYQNAELRLSIYGKSPDEWYKLAKWAIDGNVYSDNIRWLIQIPRLYDIFKTNQLMSSFQQILDNVFKPLFEATNNPSKHPEIHTFLQYVIGFDSVDDESKPENPLFDGDVTPPADWTDVENPPYAYYIYYMYANMTVLNHFRAARGMNTFVLRPHCGEAGPVQHLVCGYLMAENISHGLLLRKVPVLQYLYYLAQIGIAMSPLSNNSLFLNYDRNPFPEYLARGLVVSLSTDDPLQFHYTKEPLMEEYSIAAQVWKLSSCDMCELARNSVLMSGFPHKMKQHWLGPNYTREGVAGNDITRTNVPDIRVAFRYESLLDELANIFKVNNEQKMQYASQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V905</accession>
    <name>A0A6E8V905_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Phosphorylase b kinase regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I. The alpha chain may bind calmodulin.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 13">Glycan biosynthesis; glycogen metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cell membrane</location>
            <topology evidence="2 13">Lipid-anchor</topology>
            <orientation evidence="2 13">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the phosphorylase b kinase regulatory chain family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8V905"/>
    <dbReference type="EnsemblMetazoa" id="ACON000708-RB">
        <property type="protein sequence ID" value="ACON000708-PB"/>
        <property type="gene ID" value="ACON000708"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004679"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000708"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040898"/>
    <dbReference type="UniPathway" id="UPA00163"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005964">
        <property type="term" value="C:phosphorylase kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005977">
        <property type="term" value="P:glycogen metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000004">
        <property type="entry name" value="Phosphorylase b kinase regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011613">
        <property type="entry name" value="GH15-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045583">
        <property type="entry name" value="KPBA/B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008734">
        <property type="entry name" value="PHK_A/B_su"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10749">
        <property type="entry name" value="PHOSPHORYLASE B KINASE REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10749:SF7">
        <property type="entry name" value="PHOSPHORYLASE B KINASE REGULATORY SUBUNIT ALPHA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00723">
        <property type="entry name" value="Glyco_hydro_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19292">
        <property type="entry name" value="KPBB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 13" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="10 13" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5 13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 13" id="KW-0321">Glycogen metabolism</keyword>
    <keyword evidence="11 13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12 13" id="KW-0636">Prenylation</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GH15-like" evidence="15">
        <location>
            <begin position="8"/>
            <end position="964"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphorylase b kinase regulatory subunit alpha/beta C-terminal" evidence="16">
        <location>
            <begin position="978"/>
            <end position="1173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="714"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="724"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="745"/>
            <end position="755"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU364123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF19292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000708-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1243" mass="139001" checksum="649E5AF2C1FE5815" modified="2020-08-12" version="1">MRSRSNSGVRLDYYQRIVHRLIMRHQQPVTGLFPASPQTQHAWIRDNVYCILAVWGLSMAYKKIADQDEDRAKAYELEQCCVKLMRGLLMAMMNQKEKVERFKTSQNPLDSLHAKYSSRNGQIVVGDSEWGHLQIDAISLYLLILAQMTASGLQIVFSLDEVAFIQNLVFYIESAYCIPDYGIWERGDKSNHGQPELNASSIGMAKAALEAMNELDLFGARGGSGSVIHVLADEAHKCQAVLQSMLPRESNSKELDAGLLSVVGFPAFACDDPQLIETTQEAIITRLQGRYGCKRFLRDGYKTPKEDNTRLYYERWELRMFEKIECEWPLFFCYLILNKAFQNDKQAVAEYSQKLEDILVKTEEGMKLIPELYAVPEKAVAAEYQAPGSQQRVVVGRCPFLWGQSLYILGKLLQEGFLAVGELDPLNRRLGAQKKPDVVVQVVILAEDNDIRDKLAEHGIQVQTIADVAPIEVQPARVLSHLYTYLGRNKKLGLSGRKSRDVGILSTSKLYSLKDRIFAFTPQFSDVNRFYIASDNELMIDLLKGEINFLKSAWQNLLGRPLLTLVLKTVHLDNNKVPLAMIQTMKKLKSGYISGTRVILGNLGDFINTSAITDLSFLGSQEDGYPDKLNPAVQAYLDEHLLRSFSHRASTTSIRSSGLRPKNLRRRMSVKGAIKKTRSINVDSETLGMEGNVTERRLSHVTPQWLEPNQTTVGAAGAAGAGTGAATTGPSGGSPTSRDPSPNTQAQQQQQQQQQPRYGTERVSLDDDGKKLHIQTSATAAMPKIHIQPLPRHRPTTETNFENTEVEELIAMLRETENLEEQGDILQHLVDTQGLDFNTGMLEEGRVVTVRGLLKGLYEKACQQKMWGLVRHTAGMLGKRVEDLAKAVTDLLVRQKQVTVGMPPNSEHTITAPLPEMELRAVIHEAYGDDESTAMLSQELLVYLAMFIRTEPQLFHEMLRLRVGLIIQVMAKELSRTLNCDGEQASEHLLNLSPFEMKNLLHHILSGKEFAINSVARGNISIVSCKSSRVSKKSQIGIGEPEAAEGSLIDDRQGQWLRRRRLDGALNRVPRDFYPRVWTVLERCSGLAIEGRILPQSLTQEMTPNELKFALEVETALNTIPQPEYRQLVVEALMVLTLVTEHNILASIGTIIYVEHLVHRANQLFLEDQRKSHGDAMLCCAKNKDVRETTTAGLLLCGGAAYICQHLYDSAPSGSYGTMTYMARAVASVLELPKHGDMDCTIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V941</accession>
    <name>A0A6E8V941_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_040227967.2">
        <property type="nucleotide sequence ID" value="XM_040372033.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040227983.2">
        <property type="nucleotide sequence ID" value="XM_040372049.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040227990.2">
        <property type="nucleotide sequence ID" value="XM_040372056.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8V941"/>
    <dbReference type="EnsemblMetazoa" id="ACON000795-RA">
        <property type="protein sequence ID" value="ACON000795-PA"/>
        <property type="gene ID" value="ACON000795"/>
    </dbReference>
    <dbReference type="GeneID" id="120952554"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005333"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000795"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031730"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF251">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="78"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="120"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="147"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="173"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="208"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="237"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="303"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="335"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="370"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="395"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="426"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="460"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="639"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="689"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="731"/>
            <end position="757"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000795-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="803" mass="86139" checksum="1D39E0D45FA4056F" modified="2020-08-12" version="1">MSSLIRALSWPLISQSQGGGGGPGGPGGPGSPLDGLDGLGNGAGHCKKNGGIPPSPTFQQLMAARRLLCRRYYPEGGWGWVVIVVGILVHTMTHGLQLSYGPLMANVMRYFGKPFTDTGWLGALSTAVALFISPITIAVCKRKSTRLTAVIGGLVTALGCLFTSFASQFHQLFFSYGAVVGVGVGMTRDCSTLMVAQYFKKRREFVEIFIVSGSGLGIAVMSAFIKSAIDAIGWRLGLQAVTGTVFITFILGTCYRSASLYHPQRRAILHLKNQKRKIKDKNKHDDRPPFFDFSTLRSKTVRILLASTGIGAFGINTPIFYLAHEVQKDGIGDKVMILQIYLGLAWTLGCTAFGLLVVRNSVECRIARQYLCQTAIFMCGVCILALTAVHGNYHGYVMFVWIYGIFCGGYHYSLKMYTYEKVRARNFARAWGFVQFAQAIPIALGVPISGYMNDSGSGTAGYYFSSGCAIVGSVLMFLIDLHRRSVSRHKHTRANGTRHLCVSETCPQRRKLSFSQEPDNEPGIITGNTAMMIGPELLIPPLSDGIVEPINGLDKPELTCISEEGIADMDLPDNLLDDLDYVGDCITSCNKVENYLMLSEFENNLSAEVPILLDKRGRRLSLSKTKALLGGHLGATGGGTLLGHHHPECPIEHGAGYGPPGTTPSPTMGPPGDTNGLALLHPHQGQQQHHNHHHHHPHHHHHHSHQPVNGDLAKNLPPLATKSDVAPQNTIGGGGAGGGGRTGGGEAGKPAGGGTGTAAGAAATGSSTLQPQQHCRLNSKWRPSQQVGFLNNRVISVIDEASV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VAB0</accession>
    <name>A0A6E8VAB0_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Alpha-actinin, sarcomeric</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">F-actin cross-linking protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the alpha-actinin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040224708.1">
        <property type="nucleotide sequence ID" value="XM_040368774.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VAB0"/>
    <dbReference type="EnsemblMetazoa" id="ACON001497-RC">
        <property type="protein sequence ID" value="ACON001497-PC"/>
        <property type="gene ID" value="ACON001497"/>
    </dbReference>
    <dbReference type="GeneID" id="120950598"/>
    <dbReference type="CTD" id="31166"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001185"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001497"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032575"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd21214">
        <property type="entry name" value="CH_ACTN_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21216">
        <property type="entry name" value="CH_ACTN_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00176">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000004">
        <property type="entry name" value="Actinin alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000001">
        <property type="entry name" value="Actinin alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000004">
        <property type="entry name" value="Actinin alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000005">
        <property type="entry name" value="Actinin alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000005">
        <property type="entry name" value="Actinin alpha 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000002">
        <property type="entry name" value="Actinin, alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000003">
        <property type="entry name" value="Actinin, alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000148">
        <property type="entry name" value="alpha-actinin, sarcomeric isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001589">
        <property type="entry name" value="Actinin_actin-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014837">
        <property type="entry name" value="EF-hand_Ca_insen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018159">
        <property type="entry name" value="Spectrin/alpha-actinin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002017">
        <property type="entry name" value="Spectrin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11915">
        <property type="entry name" value="SPECTRIN/FILAMIN RELATED CYTOSKELETAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08726">
        <property type="entry name" value="EFhand_Ca_insen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00435">
        <property type="entry name" value="Spectrin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01184">
        <property type="entry name" value="efhand_Ca_insen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00150">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46966">
        <property type="entry name" value="Spectrin repeat"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00019">
        <property type="entry name" value="ACTININ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00020">
        <property type="entry name" value="ACTININ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="4" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="10">
        <location>
            <begin position="36"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="10">
        <location>
            <begin position="149"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="11">
        <location>
            <begin position="751"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="11">
        <location>
            <begin position="792"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="270"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00043249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00067262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001497-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="897" mass="103643" checksum="2CC4A6D70D4C0BB8" modified="2020-08-12" version="1">MMENGGYVGQYGGEENYMEQEEEWEREGLLDPAWEKQQKKTFTAWCNSHLRKAGTSIENIEDDFRNGLKLMLLLEVISGETLPKPDRGKMRFHKIANVNKALDFIASKGVKLVSIGAEEIVDGNLKMTLGMIWTIILRFAIQDISVEEMTAKEGLLLWCQRKTAPYKNVNVQNFHLSFKDGLAFCALIHRHRPDLIDYSKLSKDNPLENLNTAFDVAEKYLDIPRMLDPDDLQNTAMPDERAVMTYVSSYYHCFSGAQKAETAANRICKVLKVNQENERLMEEYERLASDLLEWIRRTMPWLNSRQSDSTLAGVQKKLEEYRTYRRKHKPPRVEQKAKLETNFNTLQTKLRLSNRPAYMPTEGKMVSDITNSWKGLEHAEKAFEEWLLAETMRLERLEHLAQKFKHKADTHEDWTKGKEEMLQSQDFRNCKLNELKALKKKHEAFESDLAAHQDRVEQIAAIAQELNTLEYHDCASVNARCQRICDQWDRLGALTQRRRQGLDEAERILEKIDLLHLEFAKRAAPFNNWLDGAREDLVDMFIVHTMEEIQGLIQAHDQFKATLGEADKEFNVIIGLVRDAEAIVKQEQVPGGLVNPYTTLSADLISRKWSEVRALVPQRDQTLANELRKQQNNEMLRRQFAEKANAVGPWIERQMDAVTAIGMGISGSLEEQLHRLKEYEQAVYAYKPSIEELEKIHQAVQESMIFENRYTHYTMETLRVGWEQLLTSINRNINEVENQILTRDSKGITQEQLTEFRSSFNHFDKNRTGRLAPEEFKSCLVSLGYSIGKDKQGDMDFQRILAVVDPNASGYVQFDAFLDFMTRESTDTDTAEQVIDSFRILASDRPYILPDELRRELPPDQAEYCIQRMPPYKGPNAIPGALDYMSFSTALYGESDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VAC3</accession>
    <name>A0A6E8VAC3_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="3">CBM21 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VAC3"/>
    <dbReference type="EnsemblMetazoa" id="ACON001520-RA">
        <property type="protein sequence ID" value="ACON001520-PA"/>
        <property type="gene ID" value="ACON001520"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_013889"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001520"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039936"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000164">
        <property type="term" value="C:protein phosphatase type 1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001069">
        <property type="term" value="F:glycogen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008157">
        <property type="term" value="F:protein phosphatase 1 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005979">
        <property type="term" value="P:regulation of glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2440">
        <property type="entry name" value="Carbohydrate binding type-21 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005036">
        <property type="entry name" value="CBM21_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038175">
        <property type="entry name" value="CBM21_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050782">
        <property type="entry name" value="PP1_regulatory_subunit_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12307">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12307:SF48">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03370">
        <property type="entry name" value="CBM_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51159">
        <property type="entry name" value="CBM21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBM21" evidence="2">
        <location>
            <begin position="201"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001520-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="381" mass="41358" checksum="1FD6B7C53ECC3E79" modified="2020-08-12" version="1">MPAFAEMLVSQSPPVYNHSLQTPDYKPGGGGSSPGSDGNTNGGGGGGGYYYRPTSLQLTQTQTNGGPPPFQRQLTPPCLSPTGKQLPTSPRRSCLVVRPGDDATTPEESEPPHAAMKLLTIGEEDTGCAPGTGRHKKKVVFADDHGGQLTQVRVMREPSYMPPIWSLQFLAHVTQGMISPVPQEQWVVDFVQPASDYVRFRQKLDERNVSLENVIIKETEQLIVGTVKVKNLSYHKEVIIRSSCDSWKTHEDTFCTYSVVGNGAASAYLIFDTFSFKLTLPPKSRRIEFCAAYKCDGVEYWDNNDGRNYSLTNRIAPRENSGAFLSSSSASAFNSAAPNGRNGLAAAAAASDGFISPYVDPGAVLDTWSDMSVETQSGPYW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VAE1</accession>
    <name>A0A6E8VAE1_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Tropomyosin</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the tropomyosin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040230169.1">
        <property type="nucleotide sequence ID" value="XM_040374235.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VAE1"/>
    <dbReference type="EnsemblMetazoa" id="ACON001797-RA">
        <property type="protein sequence ID" value="ACON001797-PA"/>
        <property type="gene ID" value="ACON001797"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON001797-RB">
        <property type="protein sequence ID" value="ACON001797-PB"/>
        <property type="gene ID" value="ACON001797"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON001797-RC">
        <property type="protein sequence ID" value="ACON001797-PC"/>
        <property type="gene ID" value="ACON001797"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON001797-RD">
        <property type="protein sequence ID" value="ACON001797-PD"/>
        <property type="gene ID" value="ACON001797"/>
    </dbReference>
    <dbReference type="GeneID" id="120953866"/>
    <dbReference type="CTD" id="41852"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002131"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001797"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039487"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000005">
        <property type="entry name" value="Tropomyosin alpha-1 chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000001">
        <property type="entry name" value="Tropomyosin alpha-1 chain isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000001">
        <property type="entry name" value="Tropomyosin alpha-1 chain isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000533">
        <property type="entry name" value="Tropomyosin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19269">
        <property type="entry name" value="TROPOMYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00261">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00194">
        <property type="entry name" value="TROPOMYOSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00326">
        <property type="entry name" value="TROPOMYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="1"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001797-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="285" mass="32824" checksum="041FF69A44679925" modified="2020-08-12" version="1">MDAIKKKMQAMKLEKDNALDRALLCEQQARDANLRAEKAEEEARQLQKKIQAIENDLDVTQETLMQVNTKLEEKEKALQNAESEVAALNRRIQLLEEDLERSEERLASATAKLSEASAAADESERARKVLENRALADEERMDALENQLKEARFMAEEADKKYDEVARKLAMVEADLERAEERAEAGEAKIVELEEELRVVGNNLKSLEVSEEKANQREEEYKNQIKTLTTRLKEAEARAEFAERSVQKLQKEVDRLEDELVMEKEKYREIGDDLDTAFVELILKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VAE3</accession>
    <name>A0A6E8VAE3_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Tropomyosin</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the tropomyosin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040230178.1">
        <property type="nucleotide sequence ID" value="XM_040374244.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VAE3"/>
    <dbReference type="EnsemblMetazoa" id="ACON001797-RF">
        <property type="protein sequence ID" value="ACON001797-PF"/>
        <property type="gene ID" value="ACON001797"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON001797-RG">
        <property type="protein sequence ID" value="ACON001797-PG"/>
        <property type="gene ID" value="ACON001797"/>
    </dbReference>
    <dbReference type="GeneID" id="120953866"/>
    <dbReference type="CTD" id="41852"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002131"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001797"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039487"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000005">
        <property type="entry name" value="Tropomyosin alpha-1 chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000001">
        <property type="entry name" value="Tropomyosin alpha-1 chain isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000533">
        <property type="entry name" value="Tropomyosin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19269">
        <property type="entry name" value="TROPOMYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00261">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00194">
        <property type="entry name" value="TROPOMYOSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="13"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001797-PG"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="254" mass="29433" checksum="2542C53BB28CA47B" modified="2020-08-12" version="1">MAANVQQQSGNLLDVLKKKMRQTKEEMEKYKDECEEFHKKLQNEVMRREEAESEVAALNRRIQLLEEDLERSEERLASATAKLSEASAAADESERIRKALENRTNMEDDRVGILEAQLAQAKLIAEEADKKYEEVARKLVLMEQDLERSEEKVEMNESKIVELEEELRVVGNNLKSLEVSEEKAMASRDSVEDKIHQLNDKLTNAEARAEFAERSVQKLQKEVDRLEDDLTVERAKNKMLQEEMEATLHDIQNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VCD3</accession>
    <name>A0A6E8VCD3_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON002942-RA">
        <property type="protein sequence ID" value="ACON002942-PA"/>
        <property type="gene ID" value="ACON002942"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012950"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002942"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041114"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071456">
        <property type="term" value="P:cellular response to hypoxia"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11433">
        <property type="entry name" value="bHLH-PAS_HIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000101">
        <property type="entry name" value="Similar, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000141">
        <property type="entry name" value="Similar, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001610">
        <property type="entry name" value="PAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013655">
        <property type="entry name" value="PAS_fold_3"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23043">
        <property type="entry name" value="HYPOXIA-INDUCIBLE FACTOR 1 ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23043:SF17">
        <property type="entry name" value="PROTEIN SIMILAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23171">
        <property type="entry name" value="bHLH_HIF1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08447">
        <property type="entry name" value="PAS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00086">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="9">
        <location>
            <begin position="28"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="8">
        <location>
            <begin position="132"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="8">
        <location>
            <begin position="286"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="764"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="865"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1023"/>
            <end position="1052"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1098"/>
            <end position="1140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1217"/>
            <end position="1305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="28"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="771"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="836"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="871"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1108"/>
            <end position="1135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1251"/>
            <end position="1271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="1288"/>
            <end position="1303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002942-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1341" mass="141942" checksum="0CDBB3A83B6548B6" modified="2020-08-12" version="1">MTKTVTKPADTTGKAGKPVKEKRRNNEKRKEKSRDAARCRRSRETEIFQDLASLLPMRPEEVEHLDKASVMRLSIAFLKARNMLELFPDFQSKEQITIKEDNDVSMDSMEEFQYNEAKLSLKSLGLDPETIALQTLDGFLIILSADGDVTYVSENVNEYLGISQIDIMGQPIWDYSHQCDHDELREALNGRHHSPSELLNGVANSDCRPMENRDFFLRLKCTLTSRGRSVNIKSASYKVLHVTGHIACKDKEGQRQLVAIARPLPHPANIEVPLGSSTFLTQHTLGMKFSYVDDIMLSLLGYKPSDLLGKSLYDCHHGTDAEHLMSTFKHVISKGQSETSRYRFLAKTGGYAWVVTQATVIYDKQKPHSVVCVNYVLSGVENKDEILSCAQLEARQQLLSQEPDKCAESNSNHSVVCSSSSNSNDQLLSVEPAVKTVVPIAAAVAQRAGLVEEVKAEETIAIPPRSNLAEPTVVPYVKLVANIGADQNNGNDELLASVGDKVLLCVKNDTPTVVSGEQPVPLHSRALRELNTVPATVPPKPASSTVSRPVSVTASIFTSLPPGASGTADKARAVNKPTGWGAPQSVTAKLFVTLPPPPVGAGIGGTTAGPSGSKPQHATEKIFAPRTEDMNKGFLMFSEEEPGLTMLKDEPDDLTHLAPTAGDACIPLEETGTFFNEMFEDFIIPDSYTLLQDELCSLDSQDTRLDASSIVVHPVSTSDSLTSSLSVASVASLPLGTAVSGTVDGAASLVSNAGTIAGINGSQTQQQHNYAPSSSASAASPSSLSSSSISVSSSPVPSSPSRSHISSNPNSPSGTSGLGSSASSTVSTVDGRRHSMTTIGSSNHQHNATSNDPFINYRDEVNEVSPSPHLLSPGISKSPESSSLPSLCSPNGSLPEDELAFMSLNMDELDLSMRAPYISMSEVDDLPLLTSDDLLWGVPAAAIEGNATGTSISAFGLSGKFFIKDEFTAIGSAANGTETGSSSLADPSAASLPHTVQHSLPVDKEHSLLLSAVVDTIAANGAGSVAGSSSGSNDLPTTNNSNNDSKSTAPSAASSAASIDSSLAALLCGGNVISIQSDTSNLSQQQSIGNQLLISHTNAGSQQEQRHNNQPQPQQSPLQQQQQQSSLNGSQQRQQEAGPKRIKLSEHNLLPHSMVVLPANVKLCDTWKFDDLLQLNGGSISINTKPGTQQQQQQQQQAIVPQTNIIASFVTTAVKKPSPTGVNGNNKRTLAGVSESADDTAAGNNCKRLKSSSGAGPAPSAPQLLQQLMAPSPVPQKLKSKSRSAQDGGAGGRWPNGHAGGNAGLLQEQSGNSVLMNLLVSGCDVSAGYSCFPRPSKAAKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VCH9</accession>
    <name>A0A6E8VCH9_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="12">G_PROTEIN_RECEP_F1_2 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VCH9"/>
    <dbReference type="EnsemblMetazoa" id="ACON003076-RB">
        <property type="protein sequence ID" value="ACON003076-PB"/>
        <property type="gene ID" value="ACON003076"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009037"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003076"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035453"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008188">
        <property type="term" value="F:neuropeptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
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        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24243">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24243:SF232">
        <property type="entry name" value="PYROKININ 2 RECEPTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="91"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="129"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="167"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="210"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="256"/>
            <end position="276"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="309"/>
            <end position="327"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="347"/>
            <end position="372"/>
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    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="109"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003076-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="386" mass="43343" checksum="5BA5AB4DF087FB45" modified="2020-08-12" version="1">MMELSPTVGSSGSLLAALITNGTNGRTGLLHDILDGHRTLLHQPHQPQQQQQQYYNHPHHQQYQPQYLYANESLSGSVPESASYGTDSICIILPITIFYCFIFVAGIVGNLSICVVIAKNKSMHTATNYYLFNLAVSDFLLLLFGMPQELYGTWNPFAYPFNQIACIIMGLLSETAANATVLTITSFTVERYIAICHPFRSHTMSKLSRAIKFVIVIWLVAFGLATPQALQFGVVETNTTRLCTIKNEHFSHAFEVSSFLFFVGPMTLIAVLYVLIGIKLRKSKLLQGVKRASSDYTTPPRGISAQSRVIRMLVAVVATFFICWAPFHAQRLMAVYGVFSKTENVFFYKVYMVLNYTSGILYFLSTCINPLLYNIMSHKFRDASRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VCI8</accession>
    <name>A0A6E8VCI8_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">receptor protein-tyrosine kinase</fullName>
            <ecNumber evidence="2">2.7.10.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="29 30">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="29">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8VCI8"/>
    <dbReference type="EnsemblMetazoa" id="ACON003108-RA">
        <property type="protein sequence ID" value="ACON003108-PA"/>
        <property type="gene ID" value="ACON003108"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006414"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003108"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038277"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004714">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070857">
        <property type="term" value="P:regulation of bile acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010646">
        <property type="term" value="P:regulation of cell communication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0023051">
        <property type="term" value="P:regulation of signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="Fibroblast growth factor receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000016">
        <property type="entry name" value="Fibroblast growth factor receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000020">
        <property type="entry name" value="Fibroblast growth factor receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000334">
        <property type="entry name" value="Fibroblast growth factor receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050122">
        <property type="entry name" value="RTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416:SF550">
        <property type="entry name" value="FIBROBLAST GROWTH FACTOR RECEPTOR HOMOLOG 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10 20 23" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="17" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="21" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12 25" id="KW-0472">Membrane</keyword>
    <keyword evidence="21" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 20 23" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-0675">Receptor</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 26" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 25" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 25" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="13" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="signal peptide" evidence="26">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026238516" description="receptor protein-tyrosine kinase" evidence="26">
        <location>
            <begin position="28"/>
            <end position="1013"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="566"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="28">
        <location>
            <begin position="163"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="28">
        <location>
            <begin position="360"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="28">
        <location>
            <begin position="457"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="27">
        <location>
            <begin position="674"/>
            <end position="960"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="291"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="964"/>
            <end position="995"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="24">
        <location>
            <begin position="308"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="24">
        <location>
            <begin position="974"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="19">
        <location>
            <position position="815"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <begin position="681"/>
            <end position="688"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20 23">
        <location>
            <position position="708"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="819"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="820"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="833"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for interaction with phosphotyrosine-binding proteins" evidence="22">
        <location>
            <position position="959"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00051243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR000615-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR000615-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR000615-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRSR" id="PIRSR000615-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003108-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1013" mass="113951" checksum="5CEE76FE760F5DCB" modified="2020-08-12" version="1">MSTKMASSGTDWRCILALLLSVWIVQTATYKLSQSDPDTIRINLGNKKLVKETVPLYSKLIIKCNFDSPAWYKNGKLIPVDHKRAKSLRFERVRKTDAGYYSCGSEFHEWSNLTLSVFSQETDQYQSDGAGMANLERKSLHSSSVAAAAATPILDNELKEGPPRILGTRDEPYPSPVVRGVGEAYRLKCDAVGQPAPHVSWLKDGAEYRDNSYKSTIVFKQLLPTDAGTYVCNVCNVYGCVNATTVLEVVESGEHEPAYIQHNTMESHSQLQGDPATQDTQVMMRALGRYYPAGATEKRPQHHTYQPEDADMDEDDDEDGEEEEEEEGPTNGTGVHGSDTTEDSAGAVNGSGTPPPDRAPIFTKKDQMTKIVSKPSGNMVRLRCPADGYPKPNITWTKDGRKIERAMGQVKKVNWAIVLEDLVPQDSGSYTCAVCNQVGCINFTTKLEVKDRVNHRPIITKALSNVTLVVGGNVTMECMVLSDLTKHVEWVKGLCEVNCDNITLRDPDNPEVLTLENVTHADEGWYTCIAANSLGATNESAYLQVLDELPPDDTPTAHPVRTHSTLIMGMTIFLCACFTVLAVIVIIVCKKLKREKMKHRAMEHVNQWTKKVIVLKQPVVESSIPGMSEALQMPIVRIEKQRSTLVQSGNCDPTMISEYEFPIDLNWEFPRNKLHLGKSLGEGMFGKVVMAEAHGLVKGHPSTVVAVKMLKEGHTDADVKDLVCEMEVMKMIGKHVNIINLLGCCCKDGPLYVIVEYAPHGNLKNFLRSHRFGSNYEATNEKEKKILTQKELISFAYQIARGMEHLASRRCIHRDLAARNILVSDNYVMKIADFGLARDIHDQEYYRKTTTGKLPIRWMAPESLEEKFYDSQSDVWSFGVLLWEIMTLGGNPYPSIPTWDNLLEHLKKGKRMEKPPLCSIEIYLFMRECWHYRPEERPTFSEIVQHLDRLVSITSNEEYLDLGLPQLETPPSSDDSDDDVEEDEEDCDDQAGTEHERVHMYRFNRSYNNDCIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VEH9</accession>
    <name>A0A6E8VEH9_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein acetyllysine N-acetyltransferase</fullName>
            <ecNumber evidence="2">2.3.1.286</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Regulatory protein SIR2 homolog 7</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">SIR2-like protein 7</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>N(6)-decanoyl-L-lysyl-[protein] + NAD(+) + H2O = 2''-O-decanoyl-ADP-D-ribose + nicotinamide + L-lysyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:70631"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:17932"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:143222"/>
            <dbReference type="ChEBI" id="CHEBI:189688"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:70632"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>N(6)-glutaryl-L-lysyl-[protein] + NAD(+) + H2O = 2''-O-glutaryl-ADP-D-ribose + nicotinamide + L-lysyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:47664"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:11875"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:87828"/>
            <dbReference type="ChEBI" id="CHEBI:87829"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:47665"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>N(6)-propanoyl-L-lysyl-[protein] + NAD(+) + H2O = 3''-O-propanoyl-ADP-D-ribose + nicotinamide + L-lysyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:23500"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:13758"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:138019"/>
            <dbReference type="ChEBI" id="CHEBI:145015"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:23501"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>N(6)-succinyl-L-lysyl-[protein] + NAD(+) + H2O = 2''-O-succinyl-ADP-D-ribose + nicotinamide + L-lysyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:47668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:11877"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:87830"/>
            <dbReference type="ChEBI" id="CHEBI:87832"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:47669"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the sirtuin family. Class IV subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.286" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8VEH9"/>
    <dbReference type="EnsemblMetazoa" id="ACON001220-RB">
        <property type="protein sequence ID" value="ACON001220-PB"/>
        <property type="gene ID" value="ACON001220"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011311"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001220"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032559"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097372">
        <property type="term" value="F:histone H3K18 deacetylase activity, NAD-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140861">
        <property type="term" value="P:DNA repair-dependent chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01410">
        <property type="entry name" value="SIRT7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.28.200:FF:000002">
        <property type="entry name" value="NAD-dependent deacetylase sirtuin-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1220:FF:000038">
        <property type="entry name" value="NAD-dependent protein deacetylase sirtuin-6 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050134">
        <property type="entry name" value="NAD-dep_sirtuin_deacylases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003000">
        <property type="entry name" value="Sirtuin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026590">
        <property type="entry name" value="Ssirtuin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085:SF1">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACETYLASE SIRTUIN-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACYLASE SIRTUIN-5, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02146">
        <property type="entry name" value="SIR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50305">
        <property type="entry name" value="SIRTUIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 15" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Deacetylase sirtuin-type" evidence="17">
        <location>
            <begin position="99"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="15"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="374"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="548"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="23"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="394"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="560"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="587"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="627"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="654"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="15">
        <location>
            <position position="204"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00041832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00043038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00050237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00051105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00051399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00052763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001220-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="668" mass="75768" checksum="855AEB5056DEEAFF" modified="2020-08-12" version="1">MRMKGITLSAILEQGATRRRKSPPKEECPKKQKRDSWRKVNNGFECGMIAAVLNKCESKRTHDEVRLLESEPEVVKQILERRKRNVEYKERVLETEDEPDIIEKKALKLAQAIAKANHLIVYTGAGISTSAKIPDYRGSQGIWTLLEQGKDIGEYDLSLADPTYTHMALFELHRRGILKHVLSQNCDGLHLRSGLPRFSLSEVHGNMYVEVCKQCKPNAEYWRLFDTTQFTARHYHKTNRRCRRCGGPLIDTIVHFGERGQLKWPLNWAGVTPHTEKTDLILCIGSSLKVLRKYTWLWATDRPIKKRPKIFIINLQWTPKDKVSTLKINGKCDQVMMLVMKHLNIDVPVYNRLKDPIFAHATLLLADEQHTASQPMLKKTTATTKEENTEESASSEREKETKPETSLSASTCASAQQMYLNNLILAQQISQQEKYKRILTYYKTAIQQHQQQTSTANAALPQWYDVNYAYSGLHSIIHPPPPDVNLWGCGSTLKLEPQQPLEIECKFCFENYSQHSCQFYPPQPAEFTVKSYRRGKTVVCECCDFSDGESEKYQPDMPEAAASSLASTSKAPAGDGGKTISSSSAVERTDLLEQKRRPLADEESKEADARVTKRAKCESESAVGDSASPSNVTKNGTNGAVHRPKVKPGWYGKGYRKHLRRKKRTSQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VEJ3</accession>
    <name>A0A6E8VEJ3_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">FERM, ARHGEF and pleckstrin domain-containing protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">FERM, RhoGEF and pleckstrin domain-containing protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Moesin/ezrin/radixin homolog 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="26 27">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="26">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell junction</location>
            <location evidence="5">Adherens junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Cell projection</location>
            <location evidence="6">Dendritic spine</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Filopodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="21">Cell projection</location>
            <location evidence="21">Rhabdomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="18">Synapse</location>
            <location evidence="18">Synaptosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_040223126.2">
        <property type="nucleotide sequence ID" value="XM_040367192.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VEJ3"/>
    <dbReference type="EnsemblMetazoa" id="ACON001287-RB">
        <property type="protein sequence ID" value="ACON001287-PB"/>
        <property type="gene ID" value="ACON001287"/>
    </dbReference>
    <dbReference type="GeneID" id="120949716"/>
    <dbReference type="CTD" id="40608"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001172"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001287"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032976"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043197">
        <property type="term" value="C:dendritic spine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030175">
        <property type="term" value="C:filopodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016028">
        <property type="term" value="C:rhabdomere"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008092">
        <property type="term" value="F:cytoskeletal protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009887">
        <property type="term" value="P:animal organ morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13193">
        <property type="entry name" value="FERM_C_FARP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17098">
        <property type="entry name" value="FERM_F1_FARP1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13235">
        <property type="entry name" value="PH2_FARP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000002">
        <property type="entry name" value="Band 4.1-like protein 5 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000040">
        <property type="entry name" value="FERM, RhoGEF and pleckstrin domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.10:FF:000005">
        <property type="entry name" value="FERM, RhoGEF and pleckstrin domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.900.10:FF:000021">
        <property type="entry name" value="FERM, RhoGEF and pleckstrin domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000046">
        <property type="entry name" value="FERM, RhoGEF and pleckstrin domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000798">
        <property type="entry name" value="Ez/rad/moesin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014847">
        <property type="entry name" value="FA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041788">
        <property type="entry name" value="FARP1/FARP2/FRMD7_FERM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019747">
        <property type="entry name" value="FERM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018979">
        <property type="entry name" value="FERM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018980">
        <property type="entry name" value="FERM_PH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051835">
        <property type="entry name" value="RAC1-GEF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45858">
        <property type="entry name" value="FERM DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45858:SF5">
        <property type="entry name" value="MOESIN_EZRIN_RADIXIN HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08736">
        <property type="entry name" value="FA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09380">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00373">
        <property type="entry name" value="FERM_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09379">
        <property type="entry name" value="FERM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00935">
        <property type="entry name" value="BAND41"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00661">
        <property type="entry name" value="ERMFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01195">
        <property type="entry name" value="FA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01196">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00660">
        <property type="entry name" value="FERM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="14" id="KW-0965">Cell junction</keyword>
    <keyword evidence="9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="17" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="12" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0677">Repeat</keyword>
    <keyword evidence="15" id="KW-0770">Synapse</keyword>
    <keyword evidence="11" id="KW-0771">Synaptosome</keyword>
    <feature type="domain" description="FERM" evidence="25">
        <location>
            <begin position="50"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="DH" evidence="24">
        <location>
            <begin position="652"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="23">
        <location>
            <begin position="865"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="23">
        <location>
            <begin position="1027"/>
            <end position="1124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="378"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="421"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="473"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="555"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="609"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="1128"/>
            <end position="1148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="388"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="424"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="447"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="482"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="22">
        <location>
            <begin position="498"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="510"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="559"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="609"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="22">
        <location>
            <begin position="1134"/>
            <end position="1148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00034102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00040395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00042170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00043944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001287-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1148" mass="130410" checksum="AC907299D16ED1A3" modified="2020-08-12" version="1">MSLDSMGPSAGGGSSGVGTLSGTRMIHSLSTPSGVDGTVSTSHSRGGKKLAVRIQMLDDSVTMFQVQAKATGKVLFEQVCRQLNLLEADYFGLEYQEAPSGTKYWLDLEKSLNRQVGLSLVEPVLRFCVKFYTPDPLQLEEEYTRYLFCLQVKRDLATGSLQCNDNTAALMASYIVQASCGDYAAEDYPDHTYLSSYRFVPQQDHTMQRRIMENHKKHVGQSPAEADLNLLETARRCELYGMKMHPAKDHDGVPLNLAVAHMGIAVFQGITRINTFSWAKIRKISFKRKRFLIKLHPENYVYHKDTVEFFFEGRNECKNFWKKCVENHGFFRCTAVQNVQRRKARVLSRGSSFRYSGKTQKQIIEFVRDNYVKRQTFQRSQSFRQGPLHSSNRSQSRTSCNVNQSISAHPLLPIETAEWERRSQSNVLRTPSQTRRQVDMEVDRLPSSPTGHSRSQLTEAQVETYPMKSYTQQMGMEGSGHTPLQDSSQSASPGGTWSSPYHNSNSNSSHAREADRARARQDGHSSDDYHGINGNVSLDRRSEIVQSPNRYELALGTADKSNNNSLSRGETGSYDLGSAEARARNNNGHFGGSNGGALHTVDENMINNNYQNQQPSATNGQTSSGVGLGNGNSGTLRSIDEEMRKKKWPTDRAYFLAKELLMTERTYKKDLDVINTWFREELTPEDLENLQPLFQYFESMLEHHSVFLRDLEHRILLWEGRGGHETHRIGDVMLKNMVVLPIYDEYVESHREILERLNDLYDNDDKFQQTYREFELQKVCYLPISYFILKPLHRLLHYELLLELLLAHYGEDHFDRTDCHGTLMMLTRSTEVIRRELTASENHSLLCEIQRDIDGYDGLVQPDRKLIRQGCLLKHSKRGLQQRMFFLFSDVLLYAVKSPVTQTFKVLGHVPVRSLLTENAEHNAFVIFGGQRAITVSAGTTVEKTLWLEELAKAANSLKYKPQTQLPIVSIKTCTSSEENLEACGLSSGLVPPAAARQPSPRNNTALHVCWHRGVTICLDDHLRSGRNQISGYLLRKFKNSSGWQKLWVVLTSFCLYFYKSYSDDAALASLPLLGYTVGPPGIQDAVQKEYVFKLSFKNHTYFFRAESEHTYNRWMEVLRSATQIQESRSSPTINSNNLINNNGSNGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VEJ8</accession>
    <name>A0A6E8VEJ8_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">MAP7 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MAP7 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VEJ8"/>
    <dbReference type="EnsemblMetazoa" id="ACON001315-RA">
        <property type="protein sequence ID" value="ACON001315-PA"/>
        <property type="gene ID" value="ACON001315"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000686"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001315"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034867"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015630">
        <property type="term" value="C:microtubule cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051483">
        <property type="entry name" value="MAP7_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008604">
        <property type="entry name" value="MAP7_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15073:SF18">
        <property type="entry name" value="ENSCONSIN, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15073">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05672">
        <property type="entry name" value="MAP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="100"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="136"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="183"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="247"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="339"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="681"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="710"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="811"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="883"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="57"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="137"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="183"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="249"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="282"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="293"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="345"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="409"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="480"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="710"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="811"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="883"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="900"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="928"/>
            <end position="939"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001315-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="974" mass="107556" checksum="DC9F00BC9A2268D2" modified="2020-08-12" version="1">MLTDESDRIPSTTLAVLRGILVKSLSRENLSLTDQQQLLGTRKARHVQFSLAGNRDATVQKQSRPSSANRDRLHEQSVAHTLHWFACVGDDSVDNGRNTEELQKEREERIKQMKERQNEERQKKLEELKAQALAAQKFREQKEEERRRRMDDLRRRESDRRSQVEERRRAIIEADNERREYILRKNQERDQRMETKRRNDRGSIQFAFGSSTPRMIDTSDSGMCSSFWAHRRATSITNVAYTGAALTRRSSERELTDSASKKRATSASGLDRSTDGLRSGEMTPTSLNTSRPGSAMSSSTTASGIVYRRTLPAQRKPRPASIAVTGVSASGLKEGFATSKHRLSSAGSGSITTTPAKSASGTETPTKKPLTLHSAEKRSAGARGTTPKASSTPLQSPGPEKASRNLLDSLKKSADGPKKAPTPKTPAQKDDAVVVEAKQEPPSVAAPKVEELSAQQEAILQRDDGSQQASLVEVDQSFGQEEQPQQQQEEVVVPVQQEAPAPAQTEDAELPKQAEEAVTVEVVEQAPITKQQQQAPLEQQDVSAVQAAEPTVMVAEEVPENVNGSLTVEVSGAGADAMTASMIAKRINTEEEAKAALAERRRLAREEAERLAELERKRIEAEEQAERQRILEEEERLRKLEEETIRLAEEQRRMEEERLQQAIEEARRRDEEERKRREEEARQKAEREEAERKAREEAERQRVEMAERLKKEEKEREERRKRVEAIMSRTRAKGAANNTPTKNNEDDKENAMSKSQIVLSSTPAALPTGTAMSASTISMTDSFIASEIRQEQQQQQQQTQHQSLEQAMESLSLSNNNTTSSNSSSSSSDTLANSSTINNNINNNNNNTNGGNVQFDKSVTEKENLLLASSTAGAAVVVANNNLNNGSTSNGTVVEPATNEEGTNGTKGESLLLTTNGKSEQQSPAMMASSNGSSNATTANSTSDLIIEDALMGQTNGHKNGTMDNVLFPRVSDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VEK0</accession>
    <name>A0A6E8VEK0_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Conserved oligomeric Golgi complex subunit 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the COG1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VEK0"/>
    <dbReference type="EnsemblMetazoa" id="ACON001322-RA">
        <property type="protein sequence ID" value="ACON001322-PA"/>
        <property type="gene ID" value="ACON001322"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002099"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001322"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_036908"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017119">
        <property type="term" value="C:Golgi transport complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033370">
        <property type="entry name" value="COG1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31658">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31658:SF0">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08700">
        <property type="entry name" value="VPS51_Exo84_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001322-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="866" mass="97755" checksum="C6FF93E16E2323A0" modified="2020-08-12" version="1">MAKSLDLLNIDVDQLFKQHNVAEIDLVHKRVLNEIELKREELRTMVGERYRDLLKAADTIGDMKQTAGSIIGNVDRITARCQQLNDHNLIGFRTGTDYQRMQKKHRDHNFHGVIVQIKLLTSLPEMIWSRIDREDYFVATQLFIFARHISTGLSLDTERETMRKFPVAAKQWQVLSQFFFTIEAACRESLGREELSVAVASKSLASWLLLESCPVEQTLSMFTERRSKAFLDVLDENETVYEKVKDKLLASLKVLIGTVRLFYECFVDDGTAEADGAQGGFQRELQHITGDKAAPTIGLIRSEDPMIMQMVPEMIAKFRPRLERIDLAEENVRTAVGAFLRSIETTVAGRLKRLVSLVPSIKTLHDIKRQAIALDKPSNWSAVTAKLGLSEGTDFYATFYQRLINDRMQSIIKSAWTETVQKTRADLLELLAGNPGDLKAFVWKESLDDVPMNLETALDRSNPSTRKLLMKAHGYTPALVALIGSLNGRLQTLTRDVRSFLSSSTRSEVDEMLAYFRQCCVDGVAELITATKSAEFDPTAERYALLARFLTAVRELCPALRECFIPTSIGLADSWHAGRTSSSTVAMPEEDPERWAKVAGLLEDESLQCWTLWVERFQQRWPTLSADVGYDALLNDFPVWETVTIEENDENNQPIQSTIRVPSQPSFPVQKCLHHVYELLNEAIPQTIPRPTMLDIVERLATMLLKHYDVLAGTEFVQQNQSLALQYYLDVRFLQLLLMGREQKQLNERFNELVGRFKSYIDPFDFDVFYGHLNANVKRSILKLQHFLGALICHSEQLGTIVGPSGAGTEPLDKNPNILTLSSNSLNMVWFPLLPVVCKDTASATAGSSGLTTASLGGDSLKVWHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VEK2</accession>
    <name>A0A6E8VEK2_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">furin</fullName>
            <ecNumber evidence="15">3.4.21.75</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Kex2-like endoprotease 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">dKLIP-1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="25 26">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="25">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>Release of mature proteins from their proproteins by cleavage of -Arg-Xaa-Yaa-Arg-|-Zaa- bonds, where Xaa can be any amino acid and Yaa is Arg or Lys. Releases albumin, complement component C3 and von Willebrand factor from their respective precursors.</text>
            <dbReference type="EC" id="3.4.21.75"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase S8 family. Furin subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.75" evidence="15"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8VEK2"/>
    <dbReference type="EnsemblMetazoa" id="ACON001330-RC">
        <property type="protein sequence ID" value="ACON001330-PC"/>
        <property type="gene ID" value="ACON001330"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009338"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001330"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041309"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097688">
        <property type="term" value="P:glutamate receptor clustering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016486">
        <property type="term" value="P:peptide hormone processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001941">
        <property type="term" value="P:postsynaptic membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097090">
        <property type="term" value="P:presynaptic membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008039">
        <property type="term" value="P:synaptic target recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd04059">
        <property type="entry name" value="Peptidases_S8_Protein_convertases_Kexins_Furin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000001">
        <property type="entry name" value="Furin 2, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000006">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.850:FF:000001">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.850">
        <property type="entry name" value="Peptidase S8, pro-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034182">
        <property type="entry name" value="Kexin/furin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002884">
        <property type="entry name" value="P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032815">
        <property type="entry name" value="S8_pro-domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038466">
        <property type="entry name" value="S8_pro-domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42884:SF3">
        <property type="entry name" value="FURIN-LIKE PROTEASE 1, ISOFORMS 1_1-X_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42884">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01483">
        <property type="entry name" value="P_proprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16470">
        <property type="entry name" value="S8_pro-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51829">
        <property type="entry name" value="P_HOMO_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7 20 21" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 20 21" id="KW-0645">Protease</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 20 21" id="KW-0720">Serine protease</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="23" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11" id="KW-0865">Zymogen</keyword>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="997"/>
            <end position="1019"/>
        </location>
    </feature>
    <feature type="domain" description="P/Homo B" evidence="24">
        <location>
            <begin position="607"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="226"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="955"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="1050"/>
            <end position="1109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="22">
        <location>
            <begin position="42"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="22">
        <location>
            <begin position="249"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="975"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="22">
        <location>
            <begin position="1066"/>
            <end position="1092"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="1096"/>
            <end position="1109"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="19 20">
        <location>
            <position position="317"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="19 20">
        <location>
            <position position="358"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="19 20">
        <location>
            <position position="532"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00035756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00038993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00053600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00076029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00077026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU003355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS51829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001330-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1109" mass="120899" checksum="D7C284CF3E59D74E" modified="2020-08-12" version="1">MLEQHGGVLRCTSRSDSYFPDERSADQDDPEDPQNELRWHQGSSSSSSDDCSSGSNESSCSAGGCHTRRPRCQSVTVHPRYQQHHLHPRASPWSRIIQFQLVLVLSVVLVFSGINSAVNCDENVTTGVRGSGDGGELKKGLLAGILATANSSSSSSSSSSSDDEAKLLSVNEIDEMLDGVRHFTHHWAVHIPEGDVHGLADRVAAEHGFINRGKIFHEHYHFEHSRLHKRSLSPSKPHQQRLESDRRVRWAKQQRARSRQKRDFRPLTKSYQLPIQLNDPKWPEMWYLNRGNGLDMNVIPAWKEGVTGKGVVVTILDDGLESDHPDLEHNYDPYASYDVNSSDNDPMPHYDLTDSNRHGTRCAGEVAATANNSKCAVGIAYGAKVGGVRMLDGDVTDIVEARSLGHNSQHIDIYSASWGPDDDGKTVDGPGELATRAFIEGVRNGRGGKGSIFIWASGNGGREHDNCNCDGYTNSIWTLSISSTSQEGQVPWFSEMCSSTLATTYSSGNTNEKQVITTDLHHTCTSSHTGTSASAPLAAGIAALVLEANRNLTWRDLQHIVVRTAKPGNLIDPNWSVNGVGRRVSHSFGYGLMDAAAMVRLARTWKTVPEQQVCEINARHLDKQIPPRTKVTLQLIVEHCMGVNYLEHVQAKITLTSQRRGDIKIFLTSPSGTRVTLLTPRSHDLSRSGFNQWPFMSVHTWGEAPHGTWQLEIHNEGRLLAQITQWDLIFYGTETPAQPNDPVRFGKPGYDSNFGGEMEHNALEFDSGVTSDQWRDMQQGHSEVQRTSSNDVTTVSCARFDAASCIECKSTAYHYKGRCYIQCPPGTYPSAPLASEANGTAASHRADIDEIAINGQRPLSPSSLRRRRRRQEQHVVAAALMNGASSAEASQRSVAHTTNPVGSPQCLQCHTTCLKCTGPQATDCLECQAAFRFQPIASGGESRICVSINDKRPGQPLPVAGPNNGSAGRVKAPDPNNTEHPPPGTASSSGLAPIYGYLPPVVFLAGVLLVAFVAIYVLWVHCFHGSPGIGEMSGPGHLAFVGDGGDGGTGSGLTSIRYDRVHTNEQEEEDEEEEYEMYGDDESASSGDDEGDLLPVSTTQTVAPTASKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VEM1</accession>
    <name>A0A6E8VEM1_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VEM1"/>
    <dbReference type="EnsemblMetazoa" id="ACON001419-RC">
        <property type="protein sequence ID" value="ACON001419-PC"/>
        <property type="gene ID" value="ACON001419"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001081"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001419"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035147"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12249">
        <property type="entry name" value="RRM1_hnRNPR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12250">
        <property type="entry name" value="RRM2_hnRNPR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12251">
        <property type="entry name" value="RRM3_hnRNPR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000175">
        <property type="entry name" value="Heterogeneous nuclear ribonucleoprotein Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000024">
        <property type="entry name" value="Heterogeneous nuclear ribonucleoprotein q isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000213">
        <property type="entry name" value="Uncharacterized protein, isoform R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041337">
        <property type="entry name" value="hnRNP_Q_AcD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006535">
        <property type="entry name" value="HnRNP_R/Q_splicing_fac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01648">
        <property type="entry name" value="hnRNP-R-Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21245">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18360">
        <property type="entry name" value="hnRNP_Q_AcD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="168"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="249"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="344"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001419-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="532" mass="59623" checksum="A46D48AB9175913B" modified="2020-08-12" version="1">MAEGNGEVVEEMANQKSGEGGDGERTQDYSKLLEYGLHKMVAGRLDDIYKTGKLAHSELDERALDALKEFHVEGALDVLDQFLDSNLEHVSNKSAFLCGVMKTYRQKVRATQQGQPAPAVTVQAKGPDEEKIKAILERTGYTLDVTTGQRKYGGPPPNWTGNTPGNGCEVFCGKIPKDMYEDELIPLFEKCGKIWDLRLMMDPMTGTNRGYAFVTFTSRDAASNAVRELNDYEIRNGKKIGVTISFNNHRLFVGNIPKNRDRDELLEEFAKHAPGLVEVIIYSSPDDKKKNRGFCFLEYESHKAASLAKRRLGTGRIKVWNCDIIVDWADPQEEPDEQTMSKVKVLYVRNLTQDTSEEKLKESFEQFGRVERVKKIKDYAFVHFEDRDNAVKAMKDLDGKEVGGSNIEVSLAKPPSDKKKKEEILRARERRMTQFLQTRIGLVNTVPSFPSMSPQHAGMMPGAMPPMRVPSGPGAIGKRKFEGGPHNQGDIKRRYGALANFGNSWGSQPLPQQPLGANGGEQWYTDTYSAWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VEM7</accession>
    <name>A0A6E8VEM7_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">CBS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="4">AMPK is a heterotrimer of an alpha catalytic subunit (PRKAA1 or PRKAA2), a beta (PRKAB1 or PRKAB2) and a gamma non-catalytic subunits (PRKAG1, PRKAG2 or PRKAG3). Interacts with FNIP1 and FNIP2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the 5'-AMP-activated protein kinase gamma subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VEM7"/>
    <dbReference type="EnsemblMetazoa" id="ACON001446-RB">
        <property type="protein sequence ID" value="ACON001446-PB"/>
        <property type="gene ID" value="ACON001446"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002448"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001446"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040820"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031588">
        <property type="term" value="C:nucleotide-activated protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019887">
        <property type="term" value="F:protein kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04618">
        <property type="entry name" value="CBS_euAMPK_gamma-like_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04641">
        <property type="entry name" value="CBS_euAMPK_gamma-like_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050511">
        <property type="entry name" value="AMPK_gamma/SDS23_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780">
        <property type="entry name" value="AMP-ACTIVATED PROTEIN KINASE, GAMMA REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780:SF35">
        <property type="entry name" value="LD22662P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0129">CBS domain</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CBS" evidence="7">
        <location>
            <begin position="466"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="7">
        <location>
            <begin position="547"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="7">
        <location>
            <begin position="620"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="7">
        <location>
            <begin position="694"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="18"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="257"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="312"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="753"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="18"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="35"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="257"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="279"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="322"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="347"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="757"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="769"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="792"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="837"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="866"/>
            <end position="880"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001446-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="904" mass="101217" checksum="BE0DAA4D33C3F1FE" modified="2020-08-12" version="1">MHGIQHLFRQHALEKQASADSWHSANSSNQMLQGSTSTMSSTASSSASQQRAPNERSHSIDESQVSLASEDYRLASSPRHIMPGGSVRSPTSPQEYYNLGISIPRGRKSPESRGSQCSELSAGANGIKRMSKRMPDPRTNAYNPSARRGSLESACSPPNHHYNTTAGYFPHDLDDIYEYKTDHQYFTHTGAYHQHPAYPLTSDHQRNLLFQRNHGFHSLDEYASSGVSSSNSSNNRLLGYRNRFDNSSFARKLTQSISQSSIDSGHSTASKSSTSNNNGHEKRSREKGGSNSHHHHHSITEMIKHLGKKAHIWPGGRTRHSSISEDHHTDEHRRGGAGDGDGMIAPPKEDFRSRSRSLDVDQTRRILDDCESTYKIYNKILREAAHMRRASAELAERRRASLGATRGLRADGTLDPYHAAILFRDSRGLPVADPFLEKVPLSDLEEDESQIFVKFFRFHKCYDLVPTSAKLVVFDTQLLVKKAFYALVYNGVRAAPLWDSKRQEFVGMLTITDFIKILKMYYKSPNASMDELEEHKLDTWRKVLQEDVKKLVSIGPDASLYDAIKMLVHNRIHRLPVIDPVTGNVLYILTHKRILRFLFLYINELPKPSYMQKTLREVRIGSYNNIETATEDTSIITALHKFVDRRVSALPMVDSEGRLTDIYAKFDVINLAAEKTYNDLDVSLKKANEHRNAWFEGVHHCTLDETLYTIMERIVRVEVHRLVVVDELKKVIGIISLSDILLYLVLRPSGDGIGDSESLRATDPRLMKAGSPAGGSSSSKLQATKRDSSNESIEEEKETTEEDAEQEQQDAAAHKTETTITPATPSNDEDGQKNEAEVEVETADDAVPELAVEELAEEAKEDPDDTASTPVDRSSANDSPVAQEMLATDVPLQSVQREVGLVSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VEM9</accession>
    <name>A0A6E8VEM9_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Mixed-lineage leukemia protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON001451-RC">
        <property type="protein sequence ID" value="ACON001451-PC"/>
        <property type="gene ID" value="ACON001451"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_014498"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001451"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040927"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="CC_AF10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15672">
        <property type="entry name" value="ePHD_AF10_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15574">
        <property type="entry name" value="PHD_AF10_AF17"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="protein AF-10 isoform X1"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="3.30.40.10:FF:000053">
        <property type="entry name" value="protein AF-10 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049773">
        <property type="entry name" value="AF10-like_CC"/>
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        <property type="entry name" value="AF10/AF17_PHD"/>
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        <property type="entry name" value="EPHD"/>
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    <dbReference type="InterPro" id="IPR050701">
        <property type="entry name" value="Histone_Mod_Regulator"/>
    </dbReference>
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        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
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        <property type="entry name" value="Znf_FYVE_PHD"/>
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    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
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    <dbReference type="PANTHER" id="PTHR13793:SF164">
        <property type="entry name" value="ALHAMBRA, ISOFORM P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13793">
        <property type="entry name" value="PHD FINGER PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PHD_2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="zf-HC5HC2H_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
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    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 8" id="KW-0863">Zinc-finger</keyword>
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            <begin position="5"/>
            <end position="57"/>
        </location>
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            <end position="181"/>
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            <end position="592"/>
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            <begin position="1587"/>
            <end position="1612"/>
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            <begin position="231"/>
            <end position="251"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="9">
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            <begin position="252"/>
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            <dbReference type="Proteomes" id="UP001105220"/>
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</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VGL3</accession>
    <name>A0A6E8VGL3_ANOCL</name>
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        <submittedName>
            <fullName evidence="3">OSK domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VGL3"/>
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        <property type="protein sequence ID" value="ACON003545-PA"/>
        <property type="gene ID" value="ACON003545"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012314"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003545"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032243"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="entry name" value="LOTUS_TDRD_OSKAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.610">
        <property type="entry name" value="LOTUS domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041966">
        <property type="entry name" value="LOTUS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033447">
        <property type="entry name" value="OSK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17182">
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</entry>
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        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
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            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
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    <reference evidence="2" key="2">
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            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <strain evidence="2">Ngousso</strain>
        </source>
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        <property type="gene ID" value="ACON003573"/>
    </dbReference>
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    <dbReference type="VEuPathDB" id="VectorBase:ACON003573"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR038602">
        <property type="entry name" value="Mite_allergen_7_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020234">
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    </dbReference>
    <dbReference type="Pfam" id="PF16984">
        <property type="entry name" value="Grp7_allergen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026136146" evidence="1">
        <location>
            <begin position="21"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003573-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="132" mass="14727" checksum="A88FBD764C586FAE" modified="2020-08-12" version="1">MRILSLVAFGLVCALASVGGYETEVEDEVQRNTAMSANMNNYIDVVMIQVGPWMNTHLVPMALPDVVEGFEHRPILITYHGELALTNGVFHTIHSVGRNGNAMMHYDRQLLRVAVGANIRQLGVSMTRNVYW</sequence>
</entry>
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    <accession>A0A6E8VGN0</accession>
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                <person name="Galvin B.D."/>
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            <dbReference type="PubMed" id="30669388"/>
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        <source>
            <strain>Mali-NIH</strain>
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        <citation type="submission" date="2020-05" db="UniProtKB">
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                <consortium name="EnsemblMetazoa"/>
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        <subcellularLocation>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
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            <end position="98"/>
        </location>
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            <end position="546"/>
        </location>
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            <end position="140"/>
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        </location>
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</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
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        </lineage>
    </organism>
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        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
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                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
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            </authorList>
            <dbReference type="PubMed" id="30669388"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
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    <reference evidence="5" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">Ngousso</strain>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="4">
        <location>
            <begin position="32"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="118"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="192"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="287"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="192"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="222"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="294"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003439-PT"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="553" mass="60495" checksum="5CE032086A48AC8A" modified="2020-08-12" version="1">MADDEQFSLCWNNFNSNLSAGFHESLQRGDLVDVTLAAEGHLVKAHRLILSVCSPYFRKMFTQVPVNQHAFIFLKDVSHSALQDLIQFMYCGEVNVKQDALPAFISTAEALQIKGLTETGDSAPTHQSPAKEEPAAAAAVPVTTATISTATIPASPASQRAKVQRNRIQSYKLESEESGDDKVVHIQATTSHHVSAQSNLSSQKRTMPQRGLQSHASKRTKMSISASSDGLDTSDSTPAQVQTVQTVQIVKQIPAQVIEPEYIELPIESINPKAEPDYTDETAEIETVDAETEQEQKLSEHDQGDADDDGNYVEDDTYGDMAMGKYEESYLTEGEEGAKPGVSGFVDSYTSDGGNATEISTQDQRSIRLADVDAHSSAPHMELLESIKKEDDDVIVKDEQPCAAHVSQLPLRNFNANEASSSGTAWDQLNAAKSSNSSGNSAIITCTRRKSMRMMGMEEASSVVLREKPKLDEGGGVTVNKFHELKKSNGSPAQRSRCVSCYNRNILESERLPKPKTKWVTTFCGDCPGKPFLCILCFSRIHCNSRIGIRELL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VGQ3</accession>
    <name>A0A6E8VGQ3_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transmembrane protein 208</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM208 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VGQ3"/>
    <dbReference type="EnsemblMetazoa" id="ACON003614-RA">
        <property type="protein sequence ID" value="ACON003614-PA"/>
        <property type="gene ID" value="ACON003614"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004354"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003614"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041830"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006624">
        <property type="term" value="P:vacuolar protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008506">
        <property type="entry name" value="SND2/TMEM208"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13505">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13505:SF7">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05620">
        <property type="entry name" value="TMEM208_SND2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="24"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="91"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003614-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="143" mass="15951" checksum="BBE0EB4357B0604E" modified="2020-08-12" version="1">MSLIATAVQFLGFAVYAELSTLAVVMTVLCVVAHVASYYFMALISKPKLTEKGDIIETGTDLNIEGGITEHVKDIVILTAGTQLVSMLSEFFWLLMLLLPIRAGWLLWQTVGKQFFQGDAAEEAPVNEKKQKKLMRKMNRARQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VH21</accession>
    <name>A0A6E8VH21_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">BK channel</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Maxi K channel</fullName>
        </alternativeName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="23">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the potassium channel family. Calcium-activated (TC 1.A.1.3) subfamily. Slo sub-subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VH21"/>
    <dbReference type="EnsemblMetazoa" id="ACON003709-RC">
        <property type="protein sequence ID" value="ACON003709-PC"/>
        <property type="gene ID" value="ACON003709"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_014063"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003709"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033153"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034702">
        <property type="term" value="C:monoatomic ion channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060072">
        <property type="term" value="F:large conductance calcium-activated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050804">
        <property type="term" value="P:modulation of chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009410">
        <property type="term" value="P:response to xenobiotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000035">
        <property type="entry name" value="Calcium-activated potassium channel slowpoke"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000005">
        <property type="entry name" value="calcium-activated potassium channel subunit alpha-1 isoform X6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000015">
        <property type="entry name" value="Calcium-activated potassium channel subunit alpha-1 isoform X7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003929">
        <property type="entry name" value="K_chnl_BK_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047871">
        <property type="entry name" value="K_chnl_Slo-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048735">
        <property type="entry name" value="Slowpoke-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10027">
        <property type="entry name" value="CALCIUM-ACTIVATED POTASSIUM CHANNEL ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10027:SF33">
        <property type="entry name" value="CALCIUM-ACTIVATED POTASSIUM CHANNEL SUBUNIT ALPHA-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03493">
        <property type="entry name" value="BK_channel_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22614">
        <property type="entry name" value="Slo-like_RCK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21014">
        <property type="entry name" value="Slowpoke_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01449">
        <property type="entry name" value="BKCHANNELA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00169">
        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16" id="KW-0407">Ion channel</keyword>
    <keyword evidence="14" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-0630">Potassium</keyword>
    <keyword evidence="8" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="11" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="29"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="109"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="148"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="172"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="231"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="267"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="298"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="22">
        <location>
            <begin position="337"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="22">
        <location>
            <begin position="784"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="678"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1123"/>
            <end position="1154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="678"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="699"/>
            <end position="730"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00029579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00031999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00060897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003709-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1154" mass="128970" checksum="A3185F21BD322A21" modified="2020-08-12" version="1">MSGCDEEQSVLTTELPPTDLDDCLRVRKWWCFLLSSIFTFLAGLLAVLLWRAFAFLCCRKEPELAPNDPKQKEQKASRQGKQDFEGTFMTEAKDWAGELISGQTTTGRILVVLVFILSIASLIIYFIDASNEEVERCQKWSNNITQQIDLAFNIFFMVYFFIRFIAASDKLWFMLEMYSFVDYFTIPPSFVSIYLDRTWIGLRFLRALRLMTVPDILQYLNILKTSSSIRLAQLVSIFISVWLTAAGIIHLLENSGDPLEFNNPQQLSYWTCVYFLIVTMSTVGYGDVYCETVLGRTFLVFFLLVGLAIFASCIPEIIDLIGTRPKYGGTLKNERGRRHIVVCGHITYESVSHFLKDFLHEDREDVDVEVVFLHRKPPDLELEGLFKRHFTTVEFFQGTIMSPIDLQRVKVHEADACLVLANKYCQDPDAEDAANIMRVISIKNYSDDIRVIIQLMQYHNKAYLLNIPSWDWKQGDDVICLAELKLGFIAQSCLAPGFSTMMANLFAMRSFKTSPDTQAWQNDYLQGTGCEMYTETLSPSFTGMTFPQASELCFTKLKLLLLAIEIKGGEEGNDSKISINPRGAKILANTQGFFIAQSADEVKRAWFYCKACHDDIKDETLIKKCKCKNFTLRKTTAILLGCDITRDREDINLSCNIDVYRSNGALLLINRNRRSNVSMTGSNQMLNSTKQVNKVKPNVNRQPPDNLISPSTYNRPTSRGSGSGAQSQNGVTLQAGIADDQSKDFDFEKTEMKYDSTGMFHWSPAKSLEECILDRNQAAMTVLNGHVVVCLFADPDSPLIGLRNLVMPLRASNFHYHELKHVVIVGSVEYIRREWKMLQNLPKISVLNGSPLSRADLRAVNVNLCDMCCILSAKVPSNDDPTLADKEAILASLNIKAMTFDDTIGVLSQRGSEQDNLTPVGSPIVLQRRGSVYGANVPMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSTTYFNQNALTLIRSLITGGATPELELILAEGAGLRGGYSTPESLSNRDRCRVGQISLYDGPLAQFGEAGKYGDLFVAALKSYGMLCIGLYRFRDTSSSCDASSKRYVITNPPDDFSLLPTDQVFVLMQFDPGLEYKPATVRGPSGGGRNQNSQAAGVGGGGSNKDDNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VH26</accession>
    <name>A0A6E8VH26_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">V-type proton ATPase subunit a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Essential component of the vacuolar proton pump (V-ATPase), a multimeric enzyme that catalyzes the translocation of protons across the membranes. Required for assembly and activity of the V-ATPase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the V-ATPase 116 kDa subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VH26"/>
    <dbReference type="EnsemblMetazoa" id="ACON003711-RA">
        <property type="protein sequence ID" value="ACON003711-PA"/>
        <property type="gene ID" value="ACON003711"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007046"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003711"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037888"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000220">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051117">
        <property type="term" value="F:ATPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002490">
        <property type="entry name" value="V-ATPase_116kDa_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026028">
        <property type="entry name" value="V-type_ATPase_116kDa_su_euka"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629:SF63">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629">
        <property type="entry name" value="VACUOLAR PROTON ATPASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01496">
        <property type="entry name" value="V_ATPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001293">
        <property type="entry name" value="ATP6V0A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 9" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="400"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="449"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="538"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="571"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="603"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="643"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="764"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="51"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003711-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="836" mass="95181" checksum="FFB5A90DD3A1E625" modified="2020-08-12" version="1">MGSLFRSEEMSLCQLFLQSEAAYACVSELGELGLVQFRDLNPDVNAFQRKFVNEVRRCDEMERKLRYLEKEIKKDGIPMLDTGESPEAPQPREMIDLEATFEKLENELREVNQNAEALKRNYLELTELKHILRKTQVFFDEQEGGMHTTESMTRALITDESRTGKAMGPVQLGFVAGVILRERLPAFERMLWRACRGNVFLRQAMIEDPLEDPSNGDKVYKSVFIIFFQGDQLKTRVKKICEGFRATLYPCPEAPTDRREMAMGVMTRIEDLHTVLGQTQDHRHRVLVAAAKNLKNWFVKVRKIKAIYHTLNLFNLDVTQKCLIAECWVPLLDFETIQIALRRGTERSGSSVPPILNRMETFEDPPTYNRTNKFTHAFQALINAYGVASYREMNPAPYTIITFPFLFAVMFGDLGHGTIMALFGLWMVLKEKPLAAKKSDNEIWNIFFGGRYIIFLMGVFSMYTGFVYNDIFSKSLNVFGSAWSTNYNTSTVMSNKALQLNPKGMDYAQTPYPFGLDPVWQVAPLNKIIFQNAYKMKISIIFGVIHMLFGVFVGLFNHRYFKNKMAIYCEFIPQVIFLVFLFFYMTLLMFIKWVKYSASATDVVYSEGCAPSILITFINMVLFKAPDKNTGDCLPYMFAGQSGLQKFLVVVALLCVPWMLLAKPILIMRGRKEAAVSVCIETLDDSGTQAGQQPAQQQGGGHGHDNEEMSEIFIHQGIHTIEYVLGSVSHTASYLRLWALSLAHAQLAEVLWNMVLQNGLKQDGWIGGIALWAVFAFWAVLTVGILVLMEGLSAFLHTLRLHWVEFQSKFYAGLGYAFQPFSFEVILESSSSSSED</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VH29</accession>
    <name>A0A6E8VH29_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="3">BHLH domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VH29"/>
    <dbReference type="EnsemblMetazoa" id="ACON003739-RB">
        <property type="protein sequence ID" value="ACON003739-PB"/>
        <property type="gene ID" value="ACON003739"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002760"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003739"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033789"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032502">
        <property type="term" value="P:developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050283">
        <property type="entry name" value="E-box_TF_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23349">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR, TWIST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23349:SF97">
        <property type="entry name" value="BHLH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BHLH" evidence="2">
        <location>
            <begin position="125"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="51"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003739-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="254" mass="28066" checksum="0D4F6C5150F6A84E" modified="2020-08-12" version="1">MDVLMYNAPNASYSGIGFHSFDDELMADLPSCVYAQHHQLNTVPGNGGAGSSSAGHSAGHSGHTGNAPPNLRLNPTSIRQMQHQYLHHTECLDSSTPPSPSLSTTMGTHPYNPPPYNKMQRQQTNVRERKRMMRSAPNGSINSAFDELRVHVPTFPYEKRLSKIDTLRLAIAYIALLREVLEADYDPLTYVEKCLRGEIKADRAHWNTSDLTARLSWINWENLGVHPGRRTILTSLALGSSESMGACGPPPHLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VIP1</accession>
    <name>A0A6E8VIP1_ANOCL</name>
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        <submittedName>
            <fullName evidence="2">Epimerase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VIP1"/>
    <dbReference type="EnsemblMetazoa" id="ACON004297-RA">
        <property type="protein sequence ID" value="ACON004297-PA"/>
        <property type="gene ID" value="ACON004297"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000689"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004297"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031606"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050177">
        <property type="entry name" value="Lipid_A_modif_metabolic_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43245">
        <property type="entry name" value="BIFUNCTIONAL POLYMYXIN RESISTANCE PROTEIN ARNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43245:SF11">
        <property type="entry name" value="LD23561P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="1">
        <location>
            <begin position="55"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004297-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="341" mass="38681" checksum="7FFCCB57B4D7BC88" modified="2020-08-12" version="1">MAWLNSRHTQAFADARVEFASANLINPTSCQNAFRMEEEGGEEEEKKRAQTVGGSWDYVINCAAETKPGQTDPVYQEGILKLSLNCATEAVKCKAKRYLELSTGSMCSDEKVPQKEDCPIEPWTMVGKYKAKVEQELIAIKDLPYTILRLPICYGVGDRKGLTPRIIIAGIYKHLGETMKLLWTGTMKMNVVHVEDVCGSIWELLQTDRTLHETINVCDDSDATQGLISELLADMFAIKIDYWGVTMSNMTKLDMAGAIEEINDRHLGPWAELCQRDGVQNTPLTPYMDQELLFQKHLHLSNEKLKSYGYKLRRPRITRELLEEIVQDFIDMGHFPRTLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VIP2</accession>
    <name>A0A6E8VIP2_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="4">BSD domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VIP2"/>
    <dbReference type="EnsemblMetazoa" id="ACON004273-RD">
        <property type="protein sequence ID" value="ACON004273-PD"/>
        <property type="gene ID" value="ACON004273"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011394"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004273"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030309"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048172">
        <property type="term" value="P:regulation of short-term neuronal synaptic plasticity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038203">
        <property type="term" value="P:TORC2 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3970.10:FF:000001">
        <property type="entry name" value="synapse-associated protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3970.10">
        <property type="entry name" value="BSD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005607">
        <property type="entry name" value="BSD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035925">
        <property type="entry name" value="BSD_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051494">
        <property type="entry name" value="BSD_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16019">
        <property type="entry name" value="SYNAPSE-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16019:SF6">
        <property type="entry name" value="SYNAPSE-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03909">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00751">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140383">
        <property type="entry name" value="BSD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50858">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BSD" evidence="3">
        <location>
            <begin position="285"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="15"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="115"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="353"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="487"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="32"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="366"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="423"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="500"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004273-PD"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="512" mass="54876" checksum="CA2739D0FFEE611F" modified="2020-08-12" version="1">MFSGLTNQVTSWIGAAKGEPQDEEVPTPPNSAATTATATTSGVPQRRLQLQMQLPLQRVVMVVTKGYPTKGGVFSGVSSKVTGWLGNASIPSMPAIPTVSMPAMPAMPSMPSIPGLRKANQTDENGGITNEGLVTSPEKASQGIAGSTDAADEEDRSSATGGADSRPATGPGTPTEENAGQIGQGNKAMGRKTGENVVTAGAKSIGSFLYSSFNKAGDKIKHLKDNSILGEFSKEQEAFIKNQQGGGSAGACPWTGHANEAKIKEEILSLSADRRNFVRAPPAGVEFDFDYDSSYPVALAIMNDDKELEKMRFELVPKIITEENFWRNYFYRVSLICQAADLGTLGDNNEFVKRGASEDTEDTERTSSVGSMLNQSGDISSPATSPEEQTSASPLSPEQSPPAQTNTSDLQSGVTNAAASKPTHREFISDSFHTNEQDLEEVKAGMKKLGIDSLAQQANESIGLEHEKKEQWEKDLEAELQDYELVNDGSNERNSNWEKDVDELLEDEADLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VIP4</accession>
    <name>A0A6E8VIP4_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Calcineurin is a calcium-binding and calmodulin-binding protein found in all cells from yeast to mammals, and a calcium-dependent, calmodulin-stimulated protein phosphatase.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the calcineurin regulatory subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VIP4"/>
    <dbReference type="EnsemblMetazoa" id="ACON004298-RC">
        <property type="protein sequence ID" value="ACON004298-PC"/>
        <property type="gene ID" value="ACON004298"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010643"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004298"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031110"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030431">
        <property type="term" value="P:sleep"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000047">
        <property type="entry name" value="Calcineurin subunit B type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45942">
        <property type="entry name" value="PROTEIN PHOSPATASE 3 REGULATORY SUBUNIT B ALPHA ISOFORM TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="35"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="72"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="113"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00053611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004298-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="155" mass="17594" checksum="3117F4EE19F39A77" modified="2020-08-12" version="1">DADEIRRLGKRFKKLDLDNSGALSIDEFMSLPELQQNPLVQRVIDIFDADGNGEVDFKEFIQGVSQFSVKGDKLSKLKFAFRIYDMDNDGYISNGELFQVLKMMVGNNLKDTQLQQIVDKTIVFADKDEDGKISFDEFCSVVGNTDIHKKMVVDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VIR6</accession>
    <name>A0A6E8VIR6_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VIR6"/>
    <dbReference type="EnsemblMetazoa" id="ACON004107-RA">
        <property type="protein sequence ID" value="ACON004107-PA"/>
        <property type="gene ID" value="ACON004107"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009202"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004107"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033639"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24064">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 22 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="27"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="236"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="261"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="109"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004107-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="355" mass="41103" checksum="A503CD67882E5102" modified="2020-08-12" version="1">MDTPDDSYDEIMAQAGNDGKFQRRYNLLFNFGAICFASMSYMSIILALNKPPHNCFVPGMEQYNITDANLWKNLTLPRETDNRGQLGYSKCQMYNITEQHLQRHYSEWSFASSDIIDCAYGYEYDRTYYDRTPITEYDWICDKGFRETNIFIYNRLGELFGTVIFGHLGDTLGRRPVFYLSILIITVGRLVSMFTAAYYVVFCIAAVVGSLTAHSIFQAPLIIAMEISKSERRGHISMMQCIGWTTGLCILPMVFWATKDWFWALLIVTMPIVLFMFIPSYTIESPRWLATRGNYREAMVQFRKIATINGIRELAFDERVLEKRLANHRTDKVYGLASLFNGWRMAKNTMLVILC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VIS6</accession>
    <name>A0A6E8VIS6_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Calponin-homology (CH) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the filamin family.</text>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON004335-RC">
        <property type="protein sequence ID" value="ACON004335-PC"/>
        <property type="gene ID" value="ACON004335"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012661"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004335"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038938"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21311">
        <property type="entry name" value="CH_dFLNA-like_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21315">
        <property type="entry name" value="CH_dFLNA-like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000140">
        <property type="entry name" value="FiLamiN (Actin binding protein) homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000006">
        <property type="entry name" value="Filamin-B isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000092">
        <property type="entry name" value="Filamin-B isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000008">
        <property type="entry name" value="Filamin-B isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000007">
        <property type="entry name" value="Filamin-B isoform C"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000001">
        <property type="entry name" value="Filamin-C isoform b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000096">
        <property type="entry name" value="filamin-C isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001589">
        <property type="entry name" value="Actinin_actin-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044801">
        <property type="entry name" value="Filamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017868">
        <property type="entry name" value="Filamin/ABP280_repeat-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001298">
        <property type="entry name" value="Filamin/ABP280_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38537:SF8">
        <property type="entry name" value="FILAMIN-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38537">
        <property type="entry name" value="JITTERBUG, ISOFORM N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00630">
        <property type="entry name" value="Filamin"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00557">
        <property type="entry name" value="IG_FLMN"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00019">
        <property type="entry name" value="ACTININ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00020">
        <property type="entry name" value="ACTININ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50194">
        <property type="entry name" value="FILAMIN_REPEAT"/>
        <property type="match status" value="20"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="9">
        <location>
            <begin position="15"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="9">
        <location>
            <begin position="141"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="251"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="351"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="450"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="547"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="642"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="740"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="836"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="936"/>
            <end position="1028"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="1029"/>
            <end position="1126"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="1127"/>
            <end position="1224"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="1225"/>
            <end position="1319"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="1403"/>
            <end position="1508"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="1508"/>
            <end position="1600"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="1598"/>
            <end position="1688"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="1691"/>
            <end position="1783"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="1781"/>
            <end position="1881"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="1884"/>
            <end position="1976"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="1978"/>
            <end position="2071"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="2073"/>
            <end position="2164"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="2201"/>
            <end position="2295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1328"/>
            <end position="1350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1393"/>
            <end position="1427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1329"/>
            <end position="1342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1401"/>
            <end position="1418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004335-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="2295" mass="247589" checksum="D09C94E38E8AC71D" modified="2020-08-12" version="1">MEAERDLAEDAQWKRIQQNTFTRWANEHLKIINQTIANLESDLSDGLRLIALIEVLSQKRMSKHNKRPTFRSQKLENVSVALKFLEVEGIKIVNIDSSDIVDCKLKLIMGLIWTLILHYSISLPMWEGDENGDNLANGNGASPKQRLMNWIHRLVPDLPINNFTSDWTNGKAVGALVDAVAPGLCPDWPMWDPKDAVQNAAEAMGLADDWLNVRQLIRPEEMVNPNIDEQSMMTYLSQYPNAKLKPGAPLRPRTNHSRVRAYGPGIEPTGPTVGAPANFTVETFSAGKGNVDVAVHNPNGNPEKVDCRFNNDKNLTYSVSYIPKLEGPHKVYVKFNGRDIPKSPYEVQVESQAGDASKVTATGPGLLPDGVVVGKQTYFDITTKDAGKGTPEVIILDPANHKTSVAAKLRQVGTDQWRCEYTAKQVGLHSVNVFFCGQQIRGSPYGVRVAPVCDPRKVRASGRGLQPVGVRVNDDDVTFRIFTEGAGEGIPEVKIFGPGGVVPEHSIRKLDATTYEAQYIPLKEGRYKILVLYGSQEIPKSPFEVTVGPQKHTSIVAYGPGLKSGMVGQPACFVVETNGETGNLGFSIAGPSQAEIECHDNGDGSALVKYHPTAPGEYAVHILCDNEDIPKSPHIAQVLPKADDFHPELVKVSGPGVEKNGGVIVGKPTEFTVDATRAGSAPLEVKINDVFSNTIAHKIAQTESGMKKITYTAPSAEPVTVEVNYGGVAVPQSPFRVNVSTPLDASKVQFFGPWLEPGVKPNAATHFNVDARAAGNGLLEVNLVHEESKQKVPVRIIDNNDNTYAVEVIPPLSGTYSTNLLYGGLKVPMSPKVTVAPPVDVSKIKVDGLEPSVVMNAPTDFLVDMSKVGNDVDRSKLSCNIFDPRGNEIPSKLVPSDSADDVFRIMYTPFEAGRHTIDLLYDNVPVPGSPFVVNVKSGCDPSRCRAYGPGLESAMTDKMATFTVETRGAGAGGLSLAIEGPSEAKMSCTDNRDGSCDVEYMPTEPGEYDVTIRYADKHIPGSPFRVVVNESTRPEKVKVYGPGIEHGQVYDGLPAQFFIDCSGAGPGKIAVKLSSSEGKPIATQVQDKGDGVYGVTYVPPKEGSVITANVCFADQDVSCSPFVMTVAPAPNDVPVKVYGDATTKKALPASLPAKFQIDAPKTKAKEDINVSIKGPDGKPLVPKMEKEADGTYGVSFVPDECGPYNVSIKCGGKDVLGSPFLLQAIPTGEADKCKMVQAVPVNQEYGKKNHFAVDAREAGTGAVTCKIVSHSEASDVDIDIIEKDGFFDIFYVLKDPGDYDLDIKFGGQNIPNGTFTIKAVDNVEEEQVTKSATSSSKTTSSAISENHSYQHQESYIEQVSSVSKSKQYIQQTASEEFLVRERLVNGKHDSVSTLNSVQQLSASTTTTSNSSATNGNGTTEHDNHLNGTTQASSRFHVFKLDRLNTPQTGGTVTAEVKMPSGEVDKPVIDDNHDGTVSIRYEPKEEGIHELAVKYNGEHVQGSPFKFHVDSISSGYVTAYGPGLVHGVTGEPAQFIISTKGAGAGGLQMAVEGPSKADITYHDNKDGTVSVSYLPTAPGEYKISVRFGDKHIKGSPYFAKITGEGRKRNQISVGSCSEVTLPGVISDNDLRSLNASIQAPSGLEEPCFLKRMPTGNIGISFTPREIGEHTVSVKRLGKHIANSPFKVNVCEREVGDAKKVIVTGNALKEGKTHQDNVFSIDTRNAGYGGLSLSIEGPSKAEIQCTDKDDGTLNIAYKPTEPGYYIVNLKFADHHVTGSPFTVKVSGEGTNRQREKIQRQREAVPITEVGSQCKLTFKMPGITSFDLSATVTSPGGVSEDAEIQEIEDGLYAVHFIPKELGVHTVSVKYKQIHIPGSPFQFTVGPLKDTGAHLVKAGGPGLEHGEQGVPAEFNVWTREAGGGTLAISVEGPSKAEIEFKDRKDGSCDVSYVVSEPGDYRIGLKFNDRHIPDSPFKVYISPAMGEAHKLEVAQFPTGCVQADKPAQFMVRKNGAKGELDAKVVAPSNNEDDCFIQLIDQDQYSVRFYPRENGIHAIHVKFNGVHIPGSPYRIKVGKDDVDPAAVHASGKGLGDVKTGEKTDLIIDTCNAGAGTLAVTVDGPAKVAMDCTEVEEGYKVRYTPLLPGHYYMTIKYNQMHIVGSPFKINCTGESLAEAGGQETSSVIVETVAKVSKGGNRTGVILPIFKSDASKIQSKGMGLKKAYMGKQNQFTINAGDAGNNILFVGIYGPKGPCDEVFIKHTGRNQYTVNYLVRERGDYILLVKWGDDHIPGSPFKVEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VJ25</accession>
    <name>A0A6E8VJ25_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Protein kinase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_049461586.1">
        <property type="nucleotide sequence ID" value="XM_049605629.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VJ25"/>
    <dbReference type="EnsemblMetazoa" id="ACON004443-RA">
        <property type="protein sequence ID" value="ACON004443-PA"/>
        <property type="gene ID" value="ACON004443"/>
    </dbReference>
    <dbReference type="GeneID" id="120949236"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012945"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004443"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042823"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007276">
        <property type="term" value="P:gamete generation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090090">
        <property type="term" value="P:negative regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032436">
        <property type="term" value="P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070507">
        <property type="term" value="P:regulation of microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14137">
        <property type="entry name" value="STKc_GSK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000055">
        <property type="entry name" value="Glycogen synthase kinase-3 beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000009">
        <property type="entry name" value="Glycogen synthase kinase-3 beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050591">
        <property type="entry name" value="GSK-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039192">
        <property type="entry name" value="STKc_GSK3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24057">
        <property type="entry name" value="GLYCOGEN SYNTHASE KINASE-3 ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24057:SF0">
        <property type="entry name" value="PROTEIN KINASE SHAGGY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="419"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="86"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="211"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="745"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="114"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="216"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="748"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="760"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="807"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="817"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="830"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="449"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004443-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="842" mass="90506" checksum="5945D4685471CE61" modified="2023-06-28" version="2">MAAVAATAQEMTDSVTRAAGTAGSSTAIVPIITLPGVFRRRMENLKHNSECENDEQRHNQTLPSFADAKMAASDSGVFLSMQDIDRDPPGSGTGSVIGMTRLLSSNGGSSSGARSKEPTPRLDRDRCTTLLAGSSSATGHGGGELQRKVVQEEDPAMDGHDNDVDNDDEEVGITIRLGKKKPELRLMKDRTCQSLPDVNSARPKALPKVVTACDSTDTDGFGEADDDDDVDDDELNQASAPVRPCDTGGTSTMAPIVEQPVGSSGEEENVSSTTATTVVVPPPNNPLVKPAPVPGTGPPMVDPNVLQVFAIDPKLINKYDGLEQTLYYIDENGSPKIREKYALQRQLAEEKRQRKQQKKLERAAKFGGSLDGEPPVQCSCFSFSRLSRKLKEMCKDGSKVTTVVATPGQGPDRPQEVSYTDTKIIGNGSFGVVFQAKLCDTGELVAIKKVLQDKRFKNRELQIMRRLEHCNIVKLKYFFYSSGEKKDEVYLNLVLEYIPETVYKVARHYAKNKLTIPINYIRIYMYQLFRSLAYIHSLGICHRDIKPQNLLLNPETAVLKLCDFGSAKQLLDGEPNVSYICSRYYRAPELIFGAINYTTKIDVWSAGCVLAELLLGQPIFPGDSGVDQLVEIIKVLGTPTREQIKEMNPNYTEFKFPQIKSHPWQKVFRTRTPPEAIALVSRLLEYTPGTRITPMQACAHPFFNELREGSKYLPNGREFPPLFNFTEQELAIQPSLNLILRPRNQNEAASKAGQSSSGSMEGGSGSSGNGGAGSSSGSGGVGGSGGGASASSAGDASNLAQQSCSGAAGGSAGAGVGSDDMAAASGGDANTCQPSAATSSMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VJ34</accession>
    <name>A0A6E8VJ34_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pre-mRNA-splicing factor ISY1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ISY1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VJ34"/>
    <dbReference type="EnsemblMetazoa" id="ACON004225-RA">
        <property type="protein sequence ID" value="ACON004225-PA"/>
        <property type="gene ID" value="ACON004225"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002886"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004225"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_029913"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000350">
        <property type="term" value="P:generation of catalytic spliceosome for second transesterification step"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.660">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029012">
        <property type="entry name" value="Helix_hairpin_bin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009360">
        <property type="entry name" value="Isy1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037200">
        <property type="entry name" value="Isy1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13021">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR ISY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06246">
        <property type="entry name" value="Isy1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140102">
        <property type="entry name" value="ISY1 domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="124"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="152"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004225-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="178" mass="20379" checksum="2F939A1B345DB70F" modified="2020-08-12" version="1">GLGEFRIRDLNDEINKLLREKRHWENQISDLGGPHYRRYGPRMFDAEGREVPGNRGYKYFGAAKDLPGVRELFEQEPPPPPKKTRAELMKDIDADYYGYRDDDDGILLPLEEKAEKLAIQRAVEEWNSAKGGSKTAEKDDDEPDIYGSDAYITRDPEQQTKEDPMGLLGPRFTAHVPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VJ43</accession>
    <name>A0A6E8VJ43_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the spectrin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040222399.2">
        <property type="nucleotide sequence ID" value="XM_040366465.2"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON004440-RB">
        <property type="protein sequence ID" value="ACON004440-PB"/>
        <property type="gene ID" value="ACON004440"/>
    </dbReference>
    <dbReference type="GeneID" id="120949266"/>
    <dbReference type="CTD" id="38418"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_013971"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004440"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041458"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051693">
        <property type="term" value="P:actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd21193">
        <property type="entry name" value="CH_beta_spectrin_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21194">
        <property type="entry name" value="CH_beta_spectrin_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10571">
        <property type="entry name" value="PH_beta_spectrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00174">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00176">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000001">
        <property type="entry name" value="Actinin alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000429">
        <property type="entry name" value="Beta-H spectrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000250">
        <property type="entry name" value="Blast:Spectrin beta chain, non-erythrocytic 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000210">
        <property type="entry name" value="Karst, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000007">
        <property type="entry name" value="Spectrin alpha chain non-erythrocytic 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000013">
        <property type="entry name" value="Spectrin alpha chain, non-erythrocytic 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000017">
        <property type="entry name" value="Spectrin alpha chain, non-erythrocytic 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000011">
        <property type="entry name" value="Spectrin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000024">
        <property type="entry name" value="Spectrin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000043">
        <property type="entry name" value="Spectrin beta chain, non-erythrocytic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000135">
        <property type="entry name" value="Spectrin beta chain, non-erythrocytic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000145">
        <property type="entry name" value="Spectrin beta chain, non-erythrocytic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000149">
        <property type="entry name" value="Spectrin beta chain, non-erythrocytic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000156">
        <property type="entry name" value="Spectrin beta chain, non-erythrocytic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000277">
        <property type="entry name" value="Spectrin beta chain, non-erythrocytic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000407">
        <property type="entry name" value="Spectrin beta chain, non-erythrocytic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000152">
        <property type="entry name" value="Spectrin, beta, non-erythrocytic 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000191">
        <property type="entry name" value="Spectrin, beta, non-erythrocytic 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000159">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000164">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000216">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="26"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001589">
        <property type="entry name" value="Actinin_actin-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041681">
        <property type="entry name" value="PH_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001605">
        <property type="entry name" value="PH_dom-spectrin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018159">
        <property type="entry name" value="Spectrin/alpha-actinin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002017">
        <property type="entry name" value="Spectrin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11915">
        <property type="entry name" value="SPECTRIN/FILAMIN RELATED CYTOSKELETAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15410">
        <property type="entry name" value="PH_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00435">
        <property type="entry name" value="Spectrin"/>
        <property type="match status" value="30"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00683">
        <property type="entry name" value="SPECTRINPH"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00150">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="30"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46966">
        <property type="entry name" value="Spectrin repeat"/>
        <property type="match status" value="22"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00019">
        <property type="entry name" value="ACTININ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00020">
        <property type="entry name" value="ACTININ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0117">Actin capping</keyword>
    <keyword evidence="10" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="8" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 12" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="17">
        <location>
            <begin position="54"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="17">
        <location>
            <begin position="175"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="15">
        <location>
            <begin position="862"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="16">
        <location>
            <begin position="3821"/>
            <end position="3929"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="961"/>
            <end position="989"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="3688"/>
            <end position="3726"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="3790"/>
            <end position="3818"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="3945"/>
            <end position="4006"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="4019"/>
            <end position="4250"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="784"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="1246"/>
            <end position="1276"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="2549"/>
            <end position="2608"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="3397"/>
            <end position="3424"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="3609"/>
            <end position="3664"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="3697"/>
            <end position="3717"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="3794"/>
            <end position="3805"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="3945"/>
            <end position="3962"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="3968"/>
            <end position="3980"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="3988"/>
            <end position="4002"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="4028"/>
            <end position="4039"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="4057"/>
            <end position="4069"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="4070"/>
            <end position="4109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="4147"/>
            <end position="4168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="4169"/>
            <end position="4182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="4225"/>
            <end position="4236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="4241"/>
            <end position="4250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004440-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="4250" mass="487020" checksum="8B9981DEC3CE21FD" modified="2023-06-28" version="2">[encoded data, 4250 characters]</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VJ44</accession>
    <name>A0A6E8VJ44_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Pellino</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
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            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the pellino family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VJ44"/>
    <dbReference type="EnsemblMetazoa" id="ACON004232-RA">
        <property type="protein sequence ID" value="ACON004232-PA"/>
        <property type="gene ID" value="ACON004232"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006647"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004232"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030483"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008592">
        <property type="term" value="P:regulation of Toll signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006800">
        <property type="entry name" value="Pellino_fam"/>
    </dbReference>
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        <property type="entry name" value="Pellino_FHA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048335">
        <property type="entry name" value="Pellino_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12098">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE PELLINO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12098:SF2">
        <property type="entry name" value="PROTEIN PELLINO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04710">
        <property type="entry name" value="Pellino_FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pellino FHA" evidence="4">
        <location>
            <begin position="21"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="Pellino RING" evidence="5">
        <location>
            <begin position="187"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF20723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004232-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="327" mass="36316" checksum="EBBF3562BDFB4AF6" modified="2020-08-12" version="1">MVKRTDGTESPIITESDADTDKPRMRYGELVILGYNGFLPQGDRGRRRSKFVLYKRTESNGVKRSKHYIVQSPQSSQAILDAKQHSISYTLSRNQAVIVEYKEDPDTDMFQVGRSSESPIDFVVMDTLPGDKKDAKIVQSTISRFACRILVDRADGNKARIYAAGFDSSRNIFLGTKKDLEKLVDEINAGRPQCPVGLNTLVIPRKVSLGDHVNQPYVYLNCGHVQGQHGWGQDKNTNARRCPMCLEVGPVVTLCMGVEPAFYVDSGPPTYAFNPCGHMATEKTVKYWANVDIPHGTNGFQSVCPFCATPLVGSPGYIKLIFQDNLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VJ60</accession>
    <name>A0A6E8VJ60_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glutathione peroxidase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the glutathione peroxidase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040223965.1">
        <property type="nucleotide sequence ID" value="XM_040368031.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VJ60"/>
    <dbReference type="EnsemblMetazoa" id="ACOM043132-RA">
        <property type="protein sequence ID" value="ACOM043132-PA"/>
        <property type="gene ID" value="ACOM043132"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON004247-RC">
        <property type="protein sequence ID" value="ACON004247-PC"/>
        <property type="gene ID" value="ACON004247"/>
    </dbReference>
    <dbReference type="GeneID" id="120950196"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001342"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004247"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_043019"/>
    <dbReference type="Proteomes" id="UP000075882">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00340">
        <property type="entry name" value="GSH_Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000025">
        <property type="entry name" value="Glutathione peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000889">
        <property type="entry name" value="Glutathione_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11592">
        <property type="entry name" value="GLUTATHIONE PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11592:SF78">
        <property type="entry name" value="GLUTATHIONE PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00255">
        <property type="entry name" value="GSHPx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000303">
        <property type="entry name" value="Glutathion_perox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01011">
        <property type="entry name" value="GLUTPROXDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51355">
        <property type="entry name" value="GLUTATHIONE_PEROXID_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2 5" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="6">
        <location>
            <begin position="53"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000303-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004247-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="216" mass="24018" checksum="6051540F3E3235A7" modified="2020-08-12" version="1">MSNFTTFRTLATIGRNTLGRFQYCAFSYPNQYGVNRFYSQSTTTQTDGTMEDYKNAKSVYDFTVKDSQGADVSLEKYRGKVLLIVNIASQCGLTKGNYAELTELSQKYADKDFKILSFPCNQFGGQMPEGDGEEMVCHLRSAKAEVGDVFAKIDVNGDGAHPLYKYLKHKQGGTLGDSIKWNFAKFLVNKDGQPVDRYAPTTSPSSIVKDIDKLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VJ65</accession>
    <name>A0A6E8VJ65_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">General regulator of phagocytosis. Required to uptake Gram negative bacterium by macrophages.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_040220498.1">
        <property type="nucleotide sequence ID" value="XM_040364564.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VJ65"/>
    <dbReference type="EnsemblMetazoa" id="ACON004471-RA">
        <property type="protein sequence ID" value="ACON004471-PA"/>
        <property type="gene ID" value="ACON004471"/>
    </dbReference>
    <dbReference type="GeneID" id="120948344"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005478"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004471"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_029855"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010876">
        <property type="entry name" value="C1orf43"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21425">
        <property type="entry name" value="NICE-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21425:SF2">
        <property type="entry name" value="PROTEIN C1ORF43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07406">
        <property type="entry name" value="NICE-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="187"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004471-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="284" mass="32712" checksum="09AAF298820667AE" modified="2023-06-28" version="2">MEELSGVMIVLIIGGGVLGVTMCFIFAKRQIVRFTLRQRRGPHVAVGHDTKKSIKKEIERRIDCIQKIYYEPKLIWDDKQQDDEKYILKPDTTLPPYYWRMKAVDDVKELEREIAKQDGATRHPRDSMRAFLLTTLAAPLNGSGQRLIHQFCDMYDHARHDPNEFGEEEYQSYQRLLKKLSEATKMLKSYSSSRKSSPNRTPVKKHSKMQSLLDPARLRPPPVALTPGNAGDTIISNTRMNLSLAVQLQQQQEQQFQQPDLEENEILSISQYHGETGLSKIEMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VKT9</accession>
    <name>A0A6E8VKT9_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="10">EF-hand domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the peptidase S54 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040223037.1">
        <property type="nucleotide sequence ID" value="XM_040367103.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040223038.1">
        <property type="nucleotide sequence ID" value="XM_040367104.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VKT9"/>
    <dbReference type="EnsemblMetazoa" id="ACON005058-RB">
        <property type="protein sequence ID" value="ACON005058-PB"/>
        <property type="gene ID" value="ACON005058"/>
    </dbReference>
    <dbReference type="GeneID" id="120949675"/>
    <dbReference type="CTD" id="38169"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_013073"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005058"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034068"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1540.10:FF:000002">
        <property type="entry name" value="Rhomboid, veinlet-like 3 (Drosophila)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1540.10">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022764">
        <property type="entry name" value="Peptidase_S54_rhomboid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017213">
        <property type="entry name" value="Peptidase_S54_rhomboid_met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051739">
        <property type="entry name" value="Rhomboid_IM_Serine_Proteases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45840:SF2">
        <property type="entry name" value="PROTEIN RHOMBOID-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45840">
        <property type="entry name" value="RHOMBOID-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01694">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037470">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="68"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="116"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="153"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="176"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="208"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="239"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="270"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S54 rhomboid" evidence="9">
        <location>
            <begin position="111"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="7">
        <location>
            <position position="180"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR037470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR037470-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005058-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="317" mass="35703" checksum="0550E3654884439C" modified="2020-08-12" version="1">MSGKRSRSFKCAVHHRDREVCSENDFHLIFEDPPLFRRMVHVIAMEVLPEERDRKYYADNYSCCPPPLFVILVTFVELGFFVYHSLTLGPADPAGPVPIDSMFIYRPDKRQEVWRFLFYMVLHAGWFHLGFNLIIQLLVGLPLEMVHGSTRIGCVYLAGVLAGSLGTSVFDPEVYLVGASGGVYALLAAHLANVMLNYRNMQYGILRLLAIFLFASCDVGFAIYSRYSVEPASGAPSVSYVAHLTGALAGLTIGLLVLKNFEQKLHEQLLWWVALGVYAACTIFAIVFNLLNTVTVQRLEEESEEVFKQHLFTNFGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VKW7</accession>
    <name>A0A6E8VKW7_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Actin interacting protein 3-like C-terminal domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VKW7"/>
    <dbReference type="EnsemblMetazoa" id="ACON005037-RA">
        <property type="protein sequence ID" value="ACON005037-PA"/>
        <property type="gene ID" value="ACON005037"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002690"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005037"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035357"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1540">
        <property type="entry name" value="Actin interacting protein 3, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022782">
        <property type="entry name" value="AIP3-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051825">
        <property type="entry name" value="SRCIN1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22741:SF10">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN CG32809"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22741">
        <property type="entry name" value="P140CAP/SNIP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03915">
        <property type="entry name" value="AIP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Actin interacting protein 3-like C-terminal" evidence="4">
        <location>
            <begin position="254"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="214"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="476"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="719"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="348"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="650"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="512"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="555"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="592"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="624"/>
            <end position="646"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005037-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="790" mass="87798" checksum="90E69C60CCDC6243" modified="2020-08-12" version="1">SYFSEPEFDSEYQHQHIHKSKQPTKTPYYVGTTQTLPRGMYSDRTKGAIGVASKPIRSYGSRGNIAGPMPYTTEQLYNIPDGYMSSPERSGASRGAYEEPYYSQYGTRSATVTPIIDEEQGDISIADDQYAMYGVKNVGRIPRVPPNQMYDHTRSEDLHRIRVEHMERQLANLTGLVQKALTQNPQLPLVNNSPNILNIPGQYRNDKSVSFEKSVSFSDDIQGVPKSHSPQHSADTKPPKPAIKSSTLPRTSSQERDRLKPPPPPKPLVMIAGNQYRTDLTLAPEVYNQLRGLQKKAMDLRTEVRTLRRLTQTQAVAVREDIKDTFMRIRATLLSNSGFVWGQGDKERTNLTREEEIYKQEVIRLEKDLADLESSVEGLRGEVINRRTRVNMVAVEDMALVLSRASKTVAELKMRFPVLQQGLRNLISNEMEHVCREEAFLKDEPDRLENALRRCKKLTGTLVTLKRLASVQEQRLPFPDTAGTDETIKPPETHNNVNKHRPDDSTSDDSSQTLQNSITTKISQSQLYPSEPVSSNVGLRRLHSYPSGSDTDTSPPQPTRPTTGKPPVPERNAELLSKVNNKRVPPPPPPRTSSRSPLASPTSPHVPQHHRGQQQQQPAVTLSDCEQQQRTSEGTDSGSESVCSDNSQRQLALELRHQELLKKQRQLQEQYQRLQQMSKNAVPLAPTTMNHDIKKTGSESNLPLKMGYNMTVSGSMKNLCGGSGDPTLPFDPSQGSVANNVDHQDGGGSDSSTGGPNSLDGVAIGANGSVGTLPPHLTKTTNKVYETDIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VKY7</accession>
    <name>A0A6E8VKY7_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peroxisomal leader peptide-processing protease</fullName>
            <ecNumber evidence="1">3.4.21.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Peroxisomal protease that mediates both the removal of the leader peptide from proteins containing a PTS2 target sequence and processes several PTS1-containing proteins. Catalyzes the processing of PTS1-proteins involved in the peroxisomal beta-oxidation of fatty acids.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="1">The full-lengh TYSND1 is the active the proteolytic processing of PTS1- and PTS2-proteins and in self-cleavage, and intermolecular self-cleavage of TYSND1 down-regulates its protease activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S1B family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.-" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8VKY7"/>
    <dbReference type="EnsemblMetazoa" id="ACON005054-RA">
        <property type="protein sequence ID" value="ACON005054-PA"/>
        <property type="gene ID" value="ACON005054"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001256"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005054"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038764"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031998">
        <property type="term" value="P:regulation of fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039245">
        <property type="entry name" value="TYSND1/DEG15"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21004:SF0">
        <property type="entry name" value="PEROXISOMAL LEADER PEPTIDE-PROCESSING PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21004">
        <property type="entry name" value="SERINE PROTEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13365">
        <property type="entry name" value="Trypsin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0720">Serine protease</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR037989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005054-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="373" mass="41392" checksum="B183AF10AF2890D0" modified="2020-08-12" version="1">PVRLYAPLYASIVILSTNLAKCVSWNHIKTVIDRLRPTGSSGPNLLDEVVTVSTPFGDESFFNTLNVGHISNIFGDHECLLLLDTHLSSGCQGGAVYNRNMKLQGILIGSTFTHRNENVSFPLAISMEEIVGVALNRRNLCQSLPMALPVQAFRSVCMIDSQGCWGTGCAFELNGKRYIISCSHVLATDNITCVFNDQKTMHPKLIYKNPIFDSAYDIALMEATFEEAQSSNWFCQLANYIPRIGQRLYAVGFPVLKSLARGINFKPSIIPGRVTKYSEGLLFTDCSIQCGQSGGPIFDENGLLVAIAVSNFKSSVDNLIYNCHNMCVPVKDIYNTLERYSITRDKTSLDGLQADWNVRCKWKLKPPVILNKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VL58</accession>
    <name>A0A6E8VL58_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Guanine nucleotide-binding protein subunit alpha</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the G-alpha family. G(q) subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040227257.1">
        <property type="nucleotide sequence ID" value="XM_040371323.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VL58"/>
    <dbReference type="EnsemblMetazoa" id="ACON005079-RF">
        <property type="protein sequence ID" value="ACON005079-PF"/>
        <property type="gene ID" value="ACON005079"/>
    </dbReference>
    <dbReference type="GeneID" id="120952153"/>
    <dbReference type="CTD" id="36384"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007505"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005079"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032935"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005834">
        <property type="term" value="C:heterotrimeric G-protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001664">
        <property type="term" value="F:G protein-coupled receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031683">
        <property type="term" value="F:G-protein beta/gamma-subunit complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007188">
        <property type="term" value="P:adenylate cyclase-modulating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050793">
        <property type="term" value="P:regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00066">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.400.10:FF:000002">
        <property type="entry name" value="guanine nucleotide-binding protein G(Q) subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003977">
        <property type="entry name" value="Guanine nucleotide-binding protein G(q) subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000692">
        <property type="entry name" value="Guanine nucleotide-binding protein subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.400.10">
        <property type="entry name" value="GI Alpha 1, domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000654">
        <property type="entry name" value="Gprotein_alpha_Q"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001019">
        <property type="entry name" value="Gprotein_alpha_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011025">
        <property type="entry name" value="GproteinA_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218">
        <property type="entry name" value="GTP-BINDING PROTEIN ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218:SF329">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN G(Q) SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00503">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00318">
        <property type="entry name" value="GPROTEINA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00442">
        <property type="entry name" value="GPROTEINAQ"/>
    </dbReference>
    <dbReference type="SMART" id="SM00275">
        <property type="entry name" value="G_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47895">
        <property type="entry name" value="Transducin (alpha subunit), insertion domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51882">
        <property type="entry name" value="G_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9 11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4 10 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 9 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0564">Palmitate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0807">Transducer</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="43"/>
            <end position="48"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="149"/>
            <end position="150"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="174"/>
            <end position="180"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="199"/>
            <end position="203"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="268"/>
            <end position="271"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="325"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU369122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005079-PF"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="353" mass="41517" checksum="59A206727F687397" modified="2020-08-12" version="1">MECCLSEEAKEQKRINQEIERQLRRDKRDARRELKLLLLGTGESGKSTFIKQMRIIHGSGYSDEDKRGFIKLVYQNIFMAMQSMIRAMDLLKILYSDPANIEHAELIRSVDFETVTTFEPPYVQAIKDLWADAGIQECYDRRREYQLTDSAKYYLSDLARIEAEDFLPTEQDILRARAPTTGILEYPFDLDSIIFRMVDVGGQRSERRKWIHCFENVTSIIFLVALSEYDQILFESENENRMEESKALFKTIITYPWFQHSSVILFLNKKDLLEEKIMYSHLVDYFPEYDGPQRDAIAAREFILRMFVDLNPDSEKIIYSHFTCATDTENIRFVFAAVKDTILQSNLKEYNLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VL77</accession>
    <name>A0A6E8VL77_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Endophilin-A</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
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        <property type="evidence" value="ECO:0007669"/>
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        <property type="project" value="TreeGrafter"/>
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    </evidence>
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    </evidence>
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        <source>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="280" mass="31775" checksum="FAF44D7DB45938AE" modified="2020-08-12" version="1">MEAITEVYESQWTGSEVLYGQAKTIDTQYQQFSYKLADQVLKQLDTYALQFPEMKKKIDKRGRKLVDYDSQRHSFQSLQANAAKRKDDLKVTRGREQLEEAKSTYEVLNSELHDELPALYDSRILFLVTNLQTLFACEQQFHSETSKVYAELEAIVDKLATESQRGSYTLKKINANSNPSSPQQSPVKANLSIVNNVTNGSANANGRAATTDLPPGVLYRVKATYKYVREDVDELSFEVGDVINVVEYEDPEDQEEGWLMGNKEGTNEKGMFPANFTRPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VL81</accession>
    <name>A0A6E8VL81_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Guanine nucleotide-binding protein subunit alpha</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the G-alpha family. G(q) subfamily.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_040371326.2"/>
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    <dbReference type="EnsemblMetazoa" id="ACON005079-RD">
        <property type="protein sequence ID" value="ACON005079-PD"/>
        <property type="gene ID" value="ACON005079"/>
    </dbReference>
    <dbReference type="GeneID" id="120952153"/>
    <dbReference type="CTD" id="36384"/>
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    <dbReference type="VEuPathDB" id="VectorBase:ACON005079"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032935"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005834">
        <property type="term" value="C:heterotrimeric G-protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00066">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="guanine nucleotide-binding protein G(Q) subunit alpha"/>
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    <dbReference type="FunFam" id="3.40.50.300:FF:003977">
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="GI Alpha 1, domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000654">
        <property type="entry name" value="Gprotein_alpha_Q"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001019">
        <property type="entry name" value="Gprotein_alpha_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011025">
        <property type="entry name" value="GproteinA_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218">
        <property type="entry name" value="GTP-BINDING PROTEIN ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218:SF329">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN G(Q) SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00503">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00318">
        <property type="entry name" value="GPROTEINA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00442">
        <property type="entry name" value="GPROTEINAQ"/>
    </dbReference>
    <dbReference type="SMART" id="SM00275">
        <property type="entry name" value="G_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Transducin (alpha subunit), insertion domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51882">
        <property type="entry name" value="G_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9 11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4 10 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 9 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0564">Palmitate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0807">Transducer</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="43"/>
            <end position="48"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="149"/>
            <end position="150"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="174"/>
            <end position="180"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="199"/>
            <end position="203"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="268"/>
            <end position="271"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="325"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU369122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005079-PD"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="353" mass="41560" checksum="DA16F717C9D12087" modified="2020-08-12" version="1">MECCLSEEAKEQKRINQEIERQLRRDKRDARRELKLLLLGTGESGKSTFIKQMRIIHGSGYSDEDKRGFIKLVYQNIFMAMQSMIRAMDLLKILYSDPANIEHAELIRSVDFETVTTFEPPYVQAIKDLWADAGIQECYDRRREYQLTDSAKYYLSDLARIEAEDFLPTEQDILRARAPTTGILEYPFDLDSIIFRMVDVGGQRSERRKWIHCFENVTSIIFLVALSEYDQILFESENENRMEESKALFKTIITYPWFQHSSVILFLNKKDLLEEKIMYSHLVDYFPEYDGPKHDDVKAREFILKVYLRENPDPDRMCYSHFTCATDTENIKLVFCAVKDTIMQTALKEFNLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VL84</accession>
    <name>A0A6E8VL84_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Peptidase M24 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M24 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040241185.1">
        <property type="nucleotide sequence ID" value="XM_040385251.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VL84"/>
    <dbReference type="EnsemblMetazoa" id="ACON005108-RA">
        <property type="protein sequence ID" value="ACON005108-PA"/>
        <property type="gene ID" value="ACON005108"/>
    </dbReference>
    <dbReference type="GeneID" id="120961507"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012812"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005108"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037023"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Proliferation-associated 2G4, a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047113">
        <property type="entry name" value="PA2G4/ARX1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10804:SF11">
        <property type="entry name" value="PROLIFERATION-ASSOCIATED PROTEIN 2G4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10804">
        <property type="entry name" value="PROTEASE FAMILY M24 METHIONYL AMINOPEPTIDASE, AMINOPEPTIDASE P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Creatinase/aminopeptidase"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M24" evidence="3">
        <location>
            <begin position="16"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="304"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="314"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="333"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005108-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="357" mass="39393" checksum="A42DAA73673A9E2A" modified="2020-08-12" version="1">MLMHDTGKKYKHDEDMKKGIAFPTCLSVNNCICHFSPARNDPDYVLKENDVVKIDMGAHIDGFIAVAAHTIVVGATPENKCKGRAADVVLAAYHASQAALRLLKAGTGNYAVTDAVQKIASDFKCKPIEGMLSHQLKQFKIDGEKTIIQNPTIAQKKEHEKCDFEKYEVYAMDVLISTGEGLGKEHDTRVAIYKKTDENYMLKLKASRAFIGEVKKKYGTMPFNLRNFEEEAKAKLGVNECVTHKMVEPFQVLYEKHNEYVAQFKYTVLITANGKKVVTGHPFDENCYESEHSVQDEEMKNLLAGKVAPQSGAKAKKKRNRGKKNKGAAAGAADEEEGDSGEEDAPAATADKAEAKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VL86</accession>
    <name>A0A6E8VL86_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Down syndrome cell adhesion molecule-like protein Dscam2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON004902-RC">
        <property type="protein sequence ID" value="ACON004902-PC"/>
        <property type="gene ID" value="ACON004902"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002416"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004902"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_036439"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048812">
        <property type="term" value="P:neuron projection morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20956">
        <property type="entry name" value="IgI_4_Dscam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20958">
        <property type="entry name" value="IgI_5_Dscam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20959">
        <property type="entry name" value="IgI_6_Dscam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20954">
        <property type="entry name" value="IgI_7_Dscam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002485">
        <property type="entry name" value="AGAP004902-PA-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000333">
        <property type="entry name" value="Down syndrome cell adhesion molecule"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001823">
        <property type="entry name" value="Down syndrome cell adhesion molecule 2, isoform Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000017">
        <property type="entry name" value="Down syndrome cell adhesion molecule b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000104">
        <property type="entry name" value="Down syndrome cell adhesion molecule b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000093">
        <property type="entry name" value="Down syndrome cell adhesion molecule, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000678">
        <property type="entry name" value="Down syndrome cell adhesion molecule-like protein Dscam2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001035">
        <property type="entry name" value="Down syndrome cell adhesion molecule-like protein Dscam2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001049">
        <property type="entry name" value="Down syndrome cell adhesion molecule-like protein Dscam2"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001118">
        <property type="entry name" value="Down syndrome cell adhesion molecule-like protein Dscam2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001180">
        <property type="entry name" value="Down syndrome cell adhesion molecule-like protein Dscam2"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001446">
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    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001670">
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    </dbReference>
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        <property type="entry name" value="Neuronal cell adhesion molecule"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056754">
        <property type="entry name" value="DSCAM/DSCAML_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
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        <property type="entry name" value="FN3_sf"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
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        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170:SF56">
        <property type="entry name" value="FIBRONECTIN TYPE-III DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170">
        <property type="entry name" value="PROTEIN SIDEKICK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25059">
        <property type="entry name" value="FN3_DSCAM-DSCAML_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="I-set"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1580"/>
            <end position="1602"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="4"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="204"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="296"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="389"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="487"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="578"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="674"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="772"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="13">
        <location>
            <begin position="874"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="13">
        <location>
            <begin position="971"/>
            <end position="1071"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="13">
        <location>
            <begin position="1076"/>
            <end position="1173"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="13">
        <location>
            <begin position="1177"/>
            <end position="1272"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="1274"/>
            <end position="1362"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="13">
        <location>
            <begin position="1364"/>
            <end position="1457"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="13">
        <location>
            <begin position="1458"/>
            <end position="1561"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1689"/>
            <end position="1866"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1724"/>
            <end position="1736"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1738"/>
            <end position="1747"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1774"/>
            <end position="1790"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="1792"/>
            <end position="1805"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1818"/>
            <end position="1835"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1851"/>
            <end position="1866"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004902-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1866" mass="205032" checksum="21989A4E3031E33B" modified="2020-08-12" version="1">MEPPSRLEFSNSSGGWLDCSASGSPQPSIDWLSVDGTSVGDVGGVRRVLRNGTLVLLPFPATAYRQDIHSTIYRCVASNTVGRVISRDVQVRAVVAQAYKVDVEVLAASRGCTAILRCVVPNFVKELVRVVSWVQEPAFYIYPSLQGDGKYHLLPTGELLIHNLEYNDRYPSYRCRTMHKLTRQVVTSNSAKIRMNEQRGIVSPSVVEHTSHVAVSQDEGAVLLCVAQGCPSPEYRWYTHNGPEPIPVSSGPRIRLLGPVLAIEAVTAEDGGVYKCSASNAGGEASADLRLTVSTPIHVEITPNVLSVHMGGTAEFRCLVTANGMSAGPQHITWFKDGRQLPSSGRVGDTLQVTGVTREDKGMYQCVVRRQEGDSFQASAELQLGDAPPSLVYSFIEQTLQPGPAVSLKCSAQGNPTPQIAWTLDGFPLPTNGRFMIGQYVTVHGDVISHVNISHVMVEDGGEYSCAAENRAGKTSHSARLNIYGLPYIRLIPKVTAVAGHTLYLKCPVAGYPIEEIHWERGGRELPEDMRQKVQSDGTLEIKEVQKSLDSGVYTCWARNKQGHSARRSGEVAVIVPPNIEPFSFQDGLAEGMRTRTVCGVSKGDPPLTLKWLKDGDPLLSTLLGANVSTLDQYSSLLNIPSLSAAHSGDYTCVASNPAAEVKFTASLQVKVPPRWVVEPTDVSVERNRHIMLHCQAQGVPVPTILWKKATGSKSGDYEEVRERPYTKLLANGSLLLQHVKEDREGFYLCQANNGIGTGIGKVIQLKVNSSPYFAGQSKMVTVKKGDTAIMQCEVKGDKPINVVWLRNGKHELNPSTNYRVSIKQDATPEGISAEVQISNVDSSDSGAYFCQASNLYGRDQQLVQLQVQEPPQPPSSLEAATVSSRVVNLKWQPRGGDAAEVSKYIVEYREIDRQWQYIEISDPPAYSTIIENLKPATNYVFRIIAEGPAGRSSPSQELFIKTDPQRPAGPPLNLGARPISSTEILITWMPPSYELRHGEIQGYNIGYRSMHATTNSYNFTSISGDGEDGTGELLLGNLAKYTRYTIVAQAFNQVGPGPLSEPVTAQTMEDVPSKPPEDIRCMAQSSTSIQVSWQPPQAHHTNGLLQGYKVFYECATEDAISSGEMETRKTTSLTIHLSNLRKFSNYSIQVLAYTRMGDGVISPPSFCHTDEDAPEAPADIKIAISSPSSLYVSWLPPRDPNGIITKYNLYMRAVNGREELNNDKRNLQAHQHHFEAKQLQSHTEYQFWVSASTRVGEGKSSRVVSQLTSNRVPAKIVSFGGLTIRPWKSSASLNCMAVGQPRREWFKGDTLLRPGTQHNIQLLDSGEIIVTMLQTSDTGNYTCQVDNGIGTDRLTHNLLVQVPPGPPVLYVTSATSSSILLHWKIGSTGNAPITAFSLHYRRVHGNLEEMQLSRHASSHDLKGLFCGSTYQVYLVAHNKIGSSSASTTLNVKTQGQPPGIPPDSVLIAPNSTSVVLRLNGWPDNGCPIMYFVLQYRSVSTGLDDEWHLVSNALKPQRRFTLSGLLPSTMYRLKMEAHNVAGSTNAEYTFWTLTKDGDPPPPELVQRGHRSQIFYNNIQLLIPIIITITATIICIAFTVVCYKYRQRGRQRKDTMESQQTTDAQRDRYYATIHKVALQAGDKIPETSEDISPYATFQLSEASTLAQPHHSGPANTLLHSFMYHERAMTEGCASPPPAANSSKGRRRHSRKTEPESEESDSDPDQLTSSRTESSNQLDAKMKHSKDLDVAAGGPGDHGYNPNHYMRSIPGLIYHGTQSSTSSDLSPMSEQKSLPRRGRSRYHHLHLHNTNTTPRHKSSKAQSPIASPRSSCQNDNVPIQLPRMPSQFRPIHSSRTIGSTNSRLSPNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VL87</accession>
    <name>A0A6E8VL87_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Guanine nucleotide-binding protein subunit alpha</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the G-alpha family. G(q) subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040227254.1">
        <property type="nucleotide sequence ID" value="XM_040371320.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040227255.1">
        <property type="nucleotide sequence ID" value="XM_040371321.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VL87"/>
    <dbReference type="EnsemblMetazoa" id="ACON005079-RG">
        <property type="protein sequence ID" value="ACON005079-PG"/>
        <property type="gene ID" value="ACON005079"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON005079-RI">
        <property type="protein sequence ID" value="ACON005079-PI"/>
        <property type="gene ID" value="ACON005079"/>
    </dbReference>
    <dbReference type="GeneID" id="120952153"/>
    <dbReference type="KEGG" id="acoz:120952153"/>
    <dbReference type="CTD" id="36384"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007505"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005079"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032935"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005834">
        <property type="term" value="C:heterotrimeric G-protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001664">
        <property type="term" value="F:G protein-coupled receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031683">
        <property type="term" value="F:G-protein beta/gamma-subunit complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007188">
        <property type="term" value="P:adenylate cyclase-modulating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050793">
        <property type="term" value="P:regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00066">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.400.10:FF:000002">
        <property type="entry name" value="guanine nucleotide-binding protein G(Q) subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003977">
        <property type="entry name" value="Guanine nucleotide-binding protein G(q) subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000692">
        <property type="entry name" value="Guanine nucleotide-binding protein subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.400.10">
        <property type="entry name" value="GI Alpha 1, domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000654">
        <property type="entry name" value="Gprotein_alpha_Q"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001019">
        <property type="entry name" value="Gprotein_alpha_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011025">
        <property type="entry name" value="GproteinA_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218">
        <property type="entry name" value="GTP-BINDING PROTEIN ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218:SF329">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN G(Q) SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00503">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00318">
        <property type="entry name" value="GPROTEINA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00442">
        <property type="entry name" value="GPROTEINAQ"/>
    </dbReference>
    <dbReference type="SMART" id="SM00275">
        <property type="entry name" value="G_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47895">
        <property type="entry name" value="Transducin (alpha subunit), insertion domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51882">
        <property type="entry name" value="G_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9 11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4 10 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 9 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0564">Palmitate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0807">Transducer</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="43"/>
            <end position="48"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="149"/>
            <end position="150"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="174"/>
            <end position="180"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="199"/>
            <end position="203"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="268"/>
            <end position="271"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="325"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU369122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005079-PI"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="353" mass="41686" checksum="22E1C81E13EF0497" modified="2020-08-12" version="1">MECCLSEEAKEQKRINQEIERQLRRDKRDARRELKLLLLGTGESGKSTFIKQMRIIHGSGYSDEDKRGFIKLVYQNIFMAMQSMIRAMDLLKILYSDPANIEHAELIRSVDFETVTTFEPPYVQAIKDLWADAGIQECYDRRREYQLTDSAKYYLMEIDRVAAPNYLPTEQDILRVRVPTTGIIEYPFDLEEIRFRMVDVGGQRSERRKWIHCFENVTSIIFLVALSEYDQILFESENENRMEESKALFKTIITYPWFQHSSVILFLNKKDLLEEKIMYSHLVDYFPEYDGPQRDAIAAREFILRMFVDLNPDSEKIIYSHFTCATDTENIRFVFAAVKDTILQSNLKEYNLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VPB0</accession>
    <name>A0A6E8VPB0_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the troponin C family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040224748.1">
        <property type="nucleotide sequence ID" value="XM_040368814.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VPB0"/>
    <dbReference type="EnsemblMetazoa" id="ACON006179-RA">
        <property type="protein sequence ID" value="ACON006179-PA"/>
        <property type="gene ID" value="ACON006179"/>
    </dbReference>
    <dbReference type="GeneID" id="120950626"/>
    <dbReference type="KEGG" id="acoz:120950626"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012938"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON006179"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033198"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000336">
        <property type="entry name" value="HLH domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000103">
        <property type="entry name" value="Troponin C Ib"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF0">
        <property type="entry name" value="CALMODULIN LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="10"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="46"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="86"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="122"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON006179-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="156" mass="17610" checksum="3614FFED4EBD5546" modified="2020-08-12" version="1">MSVVDDLDKAQLELLRNAFNAFDQEKKGCIGTQMVGTILSMLGHQLDDKMLKEIIDEVDADGSGELEFEEFVTLAARFMVEEDAEAMQQELKEAFRLYDKEGNGYITTQVLREILKELDDNLTNEDLDMMIEEIDSDGSGTVDFDEFMEVMTGGDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VPB3</accession>
    <name>A0A6E8VPB3_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Zasp-like motif domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_049463240.1">
        <property type="nucleotide sequence ID" value="XM_049607283.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VPB3"/>
    <dbReference type="EnsemblMetazoa" id="ACON005807-RC">
        <property type="protein sequence ID" value="ACON005807-PC"/>
        <property type="gene ID" value="ACON005807"/>
    </dbReference>
    <dbReference type="GeneID" id="120947348"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010946"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005807"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035036"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031847">
        <property type="entry name" value="PDLI1-4/Zasp-like_mid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006643">
        <property type="entry name" value="Zasp-like_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15936">
        <property type="entry name" value="DUF4749"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00735">
        <property type="entry name" value="ZM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zasp-like motif" evidence="2">
        <location>
            <begin position="7"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="96"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="169"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="183"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="197"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="217"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="259"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005807-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="323" mass="37222" checksum="BB98CE8CB4C1238C" modified="2020-08-12" version="1">MAAMQRRLVHKQFNSPINLYSQKNIQETLDRELKLLSNGAVGIDFDDPSTTKPPSLAKSAVLAALEEEEREKSRGTGLKRVAWPPPADGPEYEVHPSQQKQQPVGQQIPLQQQQQQQQFQPQQFYHQTNPQQQQQQQQPAYQQTSFPPQQQHPHQPVERIVPIQRPEAQHQQQPLAPQYQNPLEQQPYQPQPTEAFQPLTAQQQQPLYQPAQPPQPHQQLPPTLITTLRKEPPMSQEPAPVYQTQPVAAIYQGGSNMRGDQKWPPEEYKRQSEVDNEERRKLAQQPAFRPRRQQKDYADFFAKNALNNTYPGYRAPPGTQHHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VRD9</accession>
    <name>A0A6E8VRD9_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Sodium channel protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 17">Cell membrane</location>
            <topology evidence="1 17">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the sodium channel (TC 1.A.1.10) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON007086-RA">
        <property type="protein sequence ID" value="ACON007086-PA"/>
        <property type="gene ID" value="ACON007086"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002271"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007086"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035906"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001518">
        <property type="term" value="C:voltage-gated sodium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022843">
        <property type="term" value="F:voltage-gated monoatomic cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005248">
        <property type="term" value="F:voltage-gated sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0086010">
        <property type="term" value="P:membrane depolarization during action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019228">
        <property type="term" value="P:neuronal action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13433">
        <property type="entry name" value="Na_channel_gate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000150">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000047">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000089">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000019">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000039">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000053">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000058">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000370">
        <property type="entry name" value="Sodium channel protein 60E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031649">
        <property type="entry name" value="GPHH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001696">
        <property type="entry name" value="Na_channel_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044564">
        <property type="entry name" value="Na_chnl_inactivation_gate"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043203">
        <property type="entry name" value="VGCC_Ca_Na"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10037:SF62">
        <property type="entry name" value="SODIUM CHANNEL PROTEIN 60E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10037">
        <property type="entry name" value="VOLTAGE-GATED CATION CHANNEL CALCIUM AND SODIUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16905">
        <property type="entry name" value="GPHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00170">
        <property type="entry name" value="NACHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16 17" id="KW-0407">Ion channel</keyword>
    <keyword evidence="11 17" id="KW-0406">Ion transport</keyword>
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            <begin position="22"/>
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    <feature type="transmembrane region" description="Helical" evidence="17">
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    <sequence length="2902" mass="325655" checksum="1A7FA88187C94F8A" modified="2023-06-28" version="2">MATILFNCIFLAMSETIEEAEYIFLAIYTSEMIIKMIAKGFILNKYTYLRNPWNWLDFVVITSGYATIALDVGNLAGLRTFRVLRALKTVSIMPGLKTIINALLHSFKQLAEVMTLTIFCLMVFALFALQVYMGELRNKCVKNLPSDLTNVTHEDWYMWVNDTQNWIVDEDDMPMLCGNLTGARHCPPEYTCLCIGENPNHGYTNFDNFMWSMLTTFQLITLDYWENVYNMVLATCGPMSVSFFTVVVFFGSFYLINLMLAVVALSYEEEAEITQEERRKDLIDHRDDSTFSFDPASLNVKQLSKQQRKKIDARKGVLLASYSRKKTRRRKKGKEGNGNGHSKSRSVTPSPTPSPRHSIVRPQALAVQRTKGILTVAPPPVVQQQPLPQSTQPLQQQQQQQQQQQPQQQTQQQQQNNNTLHPLGPNYRGQLLLSSRQGSSNASEGGANRESSLDDSGVVDDHEEQDVTGDPGEQHHKPLHPMSQLAAQHQPPSVVQQPLPQSQLSLPLQPHLQPQQQREVTPVTLALSPREVRIIKCNGNLAKLKQQNVYGGLHPEYLSQIVVLEDDLPDRNCDSCVQCCIDYEGWLQFQNCLYKIVKDPLFELGITLCIVLNTMFLALEHHGMNANVRDALDIGNKVFTSIFTLECILKVMALSKDFFLCGWNIFDLIIVSVSLLDLIFELIEGLTVLRGLRLLRVLKLAQSWTTMKVLLSIIISTIGALGNLTFVLVIVIYIFAVIGMQLFSKDYTPDKFAPDPVPRWNFNDFFHSFMMIFRILCGEWIEPLWDCMRAEEKEGASSCFAIFLPALVMGNFMVLNLFLALLLNSFNSEELKSKKEEVGEDSKLARSFERIRSIVRKKRNANKEKNDNYANTKLEQLVNEIVIKQREEKKKHKQTVLELQPLPASQLSLPAADLQSAEPQQQQQKYMSLPKELVPPVDYRIPGGPIYSQSYQVEDQPQVVESLNRPVSGSDFCYDIPLKDRPLRTISGSQETVSQMDDQALPRDDHHQQSAHPGGPPQITEVERKILHQMSSGFGTQQSKDEPIGPLGVGDPREFDPNDRSIHIADTAQPYDEAYLQYQKSLLNRSPSYRKSLDKISSKTSSASSSLAHSLAAKCHSPLMQEVLNAGSTYLRNSNISLIQTPTPVVVSRREDNHFLVADRMSQPASIGLPSATQSPLMGGAGGAVSAHQRSPSTSSSLRKAIAAANRLSDHSLNTLSIDHDELINQMNLKDELLNCEQKELFQFLNDEIDGSNNYFSETVGFSSAIVDADTESLLLNSRKDDVIYSPDRKISNGSLKSNLSSISNSIFQALECRRGGSISSATVDSSKPHFAVHGPVTARPTIAIDNGSLQSRRDSEDKEPLVKASEFDEIIHSFETELKSLKSLSLGRSQSQTDQASPERERRNSDSCPGRSPLLEEVVKLRRPKSSTVYRGSEGVNAAPAGVHGGGANRSSGSSSGSGGTDQSDASGQQQRSEASKRRSLEKQRNITDEEFNMGFEIKKLCDQLQAPFAATGTGGSHEHHSPQSANGVVSLGTTTAGGTISTIPIVFRRKNDFHSSFDRIKRLSLIERVEEAKDEEEHQTQPLPKVSSEKLPRKHLSKDRLETLSLKSSYSVENTNQALMEGTRQLGISIQEFQRTIGQEAGSVPAGADTAPGDQTAEKKAPLKKTVTYGDTSRQDSTQHPTPKRTPYKSYDSDAPLNLAGKPWHCLVSYVDDITVGGRRNSQGVYEDPMAFPSFGRRKAPKIPQDCFPQKCYEKCKCWDTCLKTEFGQRWYRFRQFILQYVDTPAFEWFVLVLIFASSITLCFEDIHLDKNKELKRILYWTNLVFCMIFIIEMFLKWIALGFTKYFSSFWTILDFVIVFVSVFSLLIEENENLKVLRSLRTLRALRPLRAISRWQGMRIVVNALMYAIPSIFNVLLVCLVFWLIFSIMGVQFFGGKFFKCVDEDGELLPIHIVNDKWQCYALNYSWVNSKITFDHVGMGYLALFQVATFEGWMEVMADAVDARGVDLQPQREANLYAYLYFVIFIVCGSFFTLNLFIGVIIDNFNMLKKKYEGGVLEMFLTESQKHYYTAMKKLGRKKPQKVIKRPINQFLAMFYDLSNSRRFEIAIFVLIFLNMLTMGIEHYDQPHAVFFVLEVSNAFFTTVFGLEAIVKIVGLRYHYFTVPWNVFDFLLVLASIFGILMEDIMIDLPISPTLLRVVRVFRIGRILRLIKAAKGIRKLLFALVVSLPALFNIGALLALITFIYAIIGMSLFGHVRQQGALDDMVNFETFGRSMQLLFRLMTSAGWNDVLESLMIQPPDCELALDFSINGDCGHPLLAITYFTSFIIISYMIVINMYIAIILENFNQAHQEEEIGIVEDDLEMFYIRWSKYDPHAGQFIHFNQLSDFIASLDPPLGIPKPNTVALVSFNLPISKGNKIHCLDILHALVKHVLGHVEETDNFKQLQDQMDQKFKKQFPTRKELEIVSSTRIWKRQEKAAKTIQTAWKDYLRMKRERERSPMSLDEENTQTSSPGGWQSKLSALNFLHLQVHRRGTATSSRASSRKSSRASDASDLSELAGPWLNLPLMLVSGTSDMVKDVKQQHANGLELKDAPDVKGQRRRSFYNFPFFLRHQDAVEETSTSSPQPQKRPLKDSDTNLSLTTSLEKVPVPPPATPKSKRATSFVKKKPPLERGFSAQSALRLNRNAVVPDDTLSTSAADVSILVTEPSPDVPTVPAPGETTLVHVLVHRESEEYQSELDEKGEPKVKEPRDPSRASDIKLSPGTNVDYQILGGLEGAPRPVVTICVESPMESPDQDGGGGGAASATTGAVAIPIDPEPIDVNLPRDTSNIFYDYEEQSGPAGQTVSAGEAPNVEDTAVTVEIANELAECKAGRRSPGSPGDRHEQPSDRPEADLPAQSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VTG8</accession>
    <name>A0A6E8VTG8_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Regulation of nuclear pre-mRNA domain-containing protein 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="4">Associates with the RNA polymerase II complex.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040232251.2">
        <property type="nucleotide sequence ID" value="XM_040376317.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VTG8"/>
    <dbReference type="EnsemblMetazoa" id="ACON007819-RB">
        <property type="protein sequence ID" value="ACON007819-PB"/>
        <property type="gene ID" value="ACON007819"/>
    </dbReference>
    <dbReference type="GeneID" id="120955441"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002158"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007819"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032797"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16981">
        <property type="entry name" value="CID_RPRD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="regulation of nuclear pre-mRNA domain-containing protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2560">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006569">
        <property type="entry name" value="CID_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12460">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE INHIBITOR-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12460:SF40">
        <property type="entry name" value="REGULATION OF NUCLEAR PRE-MRNA DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04818">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00582">
        <property type="entry name" value="RPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51391">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0007">Acetylation</keyword>
    <keyword evidence="1" id="KW-0488">Methylation</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CID" evidence="7">
        <location>
            <begin position="26"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="172"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="457"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="656"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="783"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="866"/>
            <end position="1015"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1055"/>
            <end position="1132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1171"/>
            <end position="1195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="172"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="186"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="457"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="659"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="681"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="783"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="813"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="874"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="907"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="967"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="1106"/>
            <end position="1117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00062892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00067342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007819-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1195" mass="125565" checksum="1666C85816F04975" modified="2020-08-12" version="1">MSTKTTTTTTTTSTGRSSSSSGGGSSGEFDVASFEKRLRDLKDTQDSIQHLSSWCLSRRAHHKKIVSSWLNVLKQVKIESRLTLFHLANDVIQNSKRKNYEYVDSWGTYLQKATTLVRDEKVKHKILRIFKIWEEREIYNEEFLADLNGLLSVTTSSAKKLQQQQQQQQQLQQQQPQQQQSQQQQNALGSNQSGATLATGPTKLTETKSVVNANSTQLDVDDYQATHLVAIVRECVKHQSDTDQTFKGLNKKPHVDVEVVMNSIKGKDRKRVEAVELEIEDHLSQYENVVDVLKTEQKSRQELLTVLEQARLFYENQRTEVKVVVNAYRNFGNRLKSMKKKLDELTVSLPSPIPSPDINAPSPVPSDGDNILPNEDSYFPMIQHQGSYMGGGPLSFDINDFGRSNSPMVMNRGYHPGPPVNASNMPTGGGGGGGGGGGGGGVIPGSQPPPGIVPHPYGGGSGGGGGGGGGGGGGFINPWTTSTPRGPPPGRGNNGGYAGNNRPPLPYGGPPMLQDYGAPQQPPGRNAGPVAQPLQPPPMPPINLDASDEYNSTWNMSMTWDGTHDSSGFQSLEAPVSPSHYDRKGGMLLDYGDGSSGDGGLMQDVDHRQMSGNLSMPSFLKEKGRLADVDHRNLISLTGSPGGPPQLKDLHELDALDDSSSSSSMPMASGMRGGMHKRKPAPQPVPAPPTVPSNDSDRGGGRGSVWAAHGNVFPVPTNDVDLRLMPPSGASSSYGVQASNSALTVVPTTDGSSEAVSNDAGDESNDLDMRIQSLEKAIAAQIASSGNHSTVPPLSLDKLDDAEGGSDLLEGGSAHDGEENEDSVHSVEGAAEGDGELNLPTLQTPVSLLEIDSFLQNFERENFDDFTKPPALLMSNQTESSQSPPATSDRSVPATGESKRNPSDNTESVDMDVSEEEEDEPVEQDESKRSPSRSSAAFHHGADAENSSEAPAKDEGQSSDSSLVRLSLNNGIGGSNNSNSNSSSTGGANNPTPPLHQRPSLLPDPTFPHGAGPKWDLPPPNLMSLPMLGNFNAVLPPPPTPGALVGKLTQPPPTAGGAIWADQPPPVGGGGADGPPSLFGTPNRPPPPLPFGSGGPKGIGNNSYHRGGGGTPRGGGRGHNRPPYFRGGGAGGMRANMMPGGGMMMGGPGGSPFAGPIVGGTGGSPGMRGGYHRGGGGNKFRGGGGGGGGNNRTGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VTH2</accession>
    <name>A0A6E8VTH2_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Rap guanine nucleotide exchange factor 4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RAPGEF2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040230438.1">
        <property type="nucleotide sequence ID" value="XM_040374504.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040230439.1">
        <property type="nucleotide sequence ID" value="XM_040374505.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VTH2"/>
    <dbReference type="EnsemblMetazoa" id="ACON007307-RB">
        <property type="protein sequence ID" value="ACON007307-PB"/>
        <property type="gene ID" value="ACON007307"/>
    </dbReference>
    <dbReference type="GeneID" id="120954006"/>
    <dbReference type="CTD" id="35588"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001694"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007307"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034969"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04437">
        <property type="entry name" value="DEP_Epac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00155">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06224">
        <property type="entry name" value="REM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.840.10:FF:000002">
        <property type="entry name" value="Rap guanine nucleotide exchange factor 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.1240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.840.10">
        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.870.10">
        <property type="entry name" value="Son of sevenless (SoS) protein Chain: S domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000591">
        <property type="entry name" value="DEP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000159">
        <property type="entry name" value="RA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008937">
        <property type="entry name" value="Ras-like_GEF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000651">
        <property type="entry name" value="Ras-like_Gua-exchang_fac_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019804">
        <property type="entry name" value="Ras_G-nucl-exch_fac_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023578">
        <property type="entry name" value="Ras_GEF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001895">
        <property type="entry name" value="RASGEF_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036964">
        <property type="entry name" value="RASGEF_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113:SF327">
        <property type="entry name" value="EXCHANGE PROTEIN DIRECTLY ACTIVATED BY CAMP, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00610">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00617">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00618">
        <property type="entry name" value="RasGEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00103">
        <property type="entry name" value="CAMPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00049">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00147">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00229">
        <property type="entry name" value="RasGEFN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48366">
        <property type="entry name" value="Ras GEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50186">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50200">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00720">
        <property type="entry name" value="RASGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50009">
        <property type="entry name" value="RASGEF_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50212">
        <property type="entry name" value="RASGEF_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEP" evidence="7">
        <location>
            <begin position="71"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="6">
        <location>
            <begin position="212"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="N-terminal Ras-GEF" evidence="9">
        <location>
            <begin position="345"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-associating" evidence="8">
        <location>
            <begin position="520"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GEF" evidence="5">
        <location>
            <begin position="627"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="21"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="21"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007307-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="865" mass="98403" checksum="BED6F79F43396CD7" modified="2020-08-12" version="1">MNDIITNCRLKNGFGQSMQTTSTVQTNVQQQPSPTKKQLSPDHPNPAAPITEIPSPAMSRMGWALRTLLVSDNTSCLKDRKVGGKFIRKCAPGTELVEWLLNLSPIVHTRTQAAGMWQALLEEGVLCHVAKEQPFKDKCFLYRFKSDEDGTAGGAPTSDDVNHANEHIREALPALLHRGPDATLRMILRKPSHERTQEELELVYEELLHIAALSHLSTSIKRELASIIVFEAHAHAGTVLFNQGDEGRSWYILLKGSVDVVIHGKGTVATLKEGDDFGKLALINDAPRAATIVLKENNCHLLRVDKEHFNRILRDVEANTLRLQEHGKDVLILERVAKQRGYSAFKYTVMSGTPQKMLEHLLETRLGGQAGPNDPFLDDFLLTHIVFMPTPLLIDELAANYHADADIDLRQASEEDQEYFLTSKRRVVQFIQRWVLAVRHAVFDDPLAVEFIEELATDVEAEPALQEEASIIHHVLTQLSRYQEDRQMNATQKWKLPPNGQPICLFSGNTHSKTVIRPDDDIIFRVYCADHTYCTLRFPMATTAEVIKACAADKLQIARLPEDLLLVEVKSSGERVVFKDNDVSVPTALSLNGRIFVSPKDHLDALTPLPEQEVPTEAPDVDFEALSTKDVAYHMTLFDWDLFWAVHEYELLYHTFGRHHFNKITSNLDVFIRRFNEIQYWVVTEIVSTTNLTKRMSLVKKFIKLAAFCKEYQNLNAFFAIVMGLSNTAVSRLSMTWDKLPSKFRKLFTEYEALIDPSRNHRAYRMSVGKLQPPVIPFMPLLLKDMTFAHEGNKTSLDGLVNFEKMHMMAQTMRAVRFCRSRHLVLDPPSPKNENEIRNYIRCFRTIDNQRVLNSMSQKVEPRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VTH4</accession>
    <name>A0A6E8VTH4_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">ATP-dependent 6-phosphofructokinase</fullName>
            <shortName evidence="15">ATP-PFK</shortName>
            <shortName evidence="15">Phosphofructokinase</shortName>
            <ecNumber evidence="15">2.7.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Phosphohexokinase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 15">Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 15 16">
            <text>beta-D-fructose 6-phosphate + ATP = beta-D-fructose 1,6-bisphosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16109"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="15">Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 15 16">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 15">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade "E" sub-subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade 'E' sub-subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.11" evidence="15"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8VTH4"/>
    <dbReference type="EnsemblMetazoa" id="ACON007642-RB">
        <property type="protein sequence ID" value="ACON007642-PB"/>
        <property type="gene ID" value="ACON007642"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON007642-RD">
        <property type="protein sequence ID" value="ACON007642-PD"/>
        <property type="gene ID" value="ACON007642"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007189"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007642"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039015"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00182"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005945">
        <property type="term" value="C:6-phosphofructokinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003872">
        <property type="term" value="F:6-phosphofructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070095">
        <property type="term" value="F:fructose-6-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048029">
        <property type="term" value="F:monosaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061621">
        <property type="term" value="P:canonical glycolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030388">
        <property type="term" value="P:fructose 1,6-bisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.460:FF:000001">
        <property type="entry name" value="ATP-dependent 6-phosphofructokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.460:FF:000003">
        <property type="entry name" value="ATP-dependent 6-phosphofructokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.450:FF:000043">
        <property type="entry name" value="ATP-dependent 6-phosphofructokinase, platelet type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.450:FF:000064">
        <property type="entry name" value="Phosphofructokinase, platelet b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.450">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.460">
        <property type="entry name" value="Phosphofructokinase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03184">
        <property type="entry name" value="Phosphofructokinase_I_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009161">
        <property type="entry name" value="6-Pfructokinase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022953">
        <property type="entry name" value="ATP_PFK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015912">
        <property type="entry name" value="Phosphofructokinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000023">
        <property type="entry name" value="Phosphofructokinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035966">
        <property type="entry name" value="PKF_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02478">
        <property type="entry name" value="6PF1K_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13697:SF4">
        <property type="entry name" value="ATP-DEPENDENT 6-PHOSPHOFRUCTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13697">
        <property type="entry name" value="PHOSPHOFRUCTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00365">
        <property type="entry name" value="PFK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000533">
        <property type="entry name" value="ATP_PFK_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00476">
        <property type="entry name" value="PHFRCTKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53784">
        <property type="entry name" value="Phosphofructokinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00433">
        <property type="entry name" value="PHOSPHOFRUCTOKINASE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 15" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="11 15 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 15" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13 15 16" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="10 15 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="12 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 15 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 15 16" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphofructokinase" evidence="17">
        <location>
            <begin position="20"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphofructokinase" evidence="17">
        <location>
            <begin position="412"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="region of interest" description="N-terminal catalytic PFK domain 1" evidence="15">
        <location>
            <begin position="1"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="C-terminal regulatory PFK domain 2" evidence="15">
        <location>
            <begin position="411"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="15">
        <location>
            <position position="168"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="90"/>
            <end position="91"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="120"/>
            <end position="123"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <begin position="166"/>
            <end position="168"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <begin position="210"/>
            <end position="212"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <position position="266"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="294"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <begin position="300"/>
            <end position="303"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <position position="480"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <begin position="537"/>
            <end position="541"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="575"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <begin position="582"/>
            <end position="584"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <position position="638"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="664"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <begin position="670"/>
            <end position="673"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <position position="745"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR000533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007642-PD"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="789" mass="86681" checksum="09860C340D6CA84F" modified="2020-08-12" version="1">MSSAPVQRFIQFGAHKGKGIAVFTSGGDSQGMNAAVRAVVRMGIYVGCKVYFIREGYQGMVDGGDNIVEANWSSVSSIIHRGGTVIGSARCKDFRERAGRLQAAHNLVSRGITNLVVIGGDGSLTGANLFRQEWGSLLDELLASNRITQDQRIKYKSLHIAGMVGSIDNDFCGTDMTIGTDSALHRIIEAIDAIVSTAYSHQRTFIMEVMGRHCGYLAIMAALASEADYVFIPEDPVVHNWPEKLCKKILQERSAGQRLNIIIVSEGAIDRDGNPITAEKVKQVVVDRLQQDTRITVLGHVQRGGNPSAFDRVLGCRMGAEAVLALMEADDKTEPCVVSLDGNQAVRVPLMECVERTKAVAKAMEDKNWELAVQLRGRSFARNLETYKMLTRLKPPKSAFDEQGRGVGGYTMAVMHIGAPACGMNAAVRSFVRNCIYRGDTVMGIHDGIEGLVAGHIKKMEWSDVTGWVGQGGAFLGTKRTLPEGKFADIAARLKEFGIQALLIVGGFEAYHAAGQITDQRSKFKEFCIPIVVIPSTISNNMPGTEFSLGADTGLNEITEICDRIRQSAQGTKRRVFVIETMGGYCGYLATLAGLAGGADAAYIYEEKFTIKDLQQDVYHMASKMSDGVQRGLILRNEKASENYNTEFIYRLYSEEGKGLFSTRMNVLGHMQQGGSPTPFDRNMGTKMAAKCVEWLVEQLKANTQPDGSINATAPETACLLGLVRRQYKFSPLKELIGETNFMQRIPKHQWWLKLRPLLRILAKHDSTYEEEGMYITVEEEMSTDSPCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VTH6</accession>
    <name>A0A6E8VTH6_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Sodium/potassium-transporting ATPase subunit beta</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the X(+)/potassium ATPases subunit beta family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040239191.1">
        <property type="nucleotide sequence ID" value="XM_040383257.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040239192.1">
        <property type="nucleotide sequence ID" value="XM_040383258.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VTH6"/>
    <dbReference type="EnsemblMetazoa" id="ACON007791-RA">
        <property type="protein sequence ID" value="ACON007791-PA"/>
        <property type="gene ID" value="ACON007791"/>
    </dbReference>
    <dbReference type="GeneID" id="120959666"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004424"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007791"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037430"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005890">
        <property type="term" value="C:sodium:potassium-exchanging ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030007">
        <property type="term" value="P:intracellular potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006883">
        <property type="term" value="P:intracellular sodium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036376">
        <property type="term" value="P:sodium ion export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1660:FF:000004">
        <property type="entry name" value="sodium/potassium-transporting ATPase subunit beta-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1660">
        <property type="entry name" value="Na, k-atpase alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000402">
        <property type="entry name" value="Na/K_ATPase_sub_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038702">
        <property type="entry name" value="Na/K_ATPase_sub_beta_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11523">
        <property type="entry name" value="SODIUM/POTASSIUM-DEPENDENT ATPASE BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11523:SF46">
        <property type="entry name" value="SODIUM_POTASSIUM-TRANSPORTING ATPASE SUBUNIT BETA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00287">
        <property type="entry name" value="Na_K-ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00390">
        <property type="entry name" value="ATPASE_NA_K_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0630">Potassium</keyword>
    <keyword evidence="5" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="11" id="KW-0915">Sodium</keyword>
    <keyword evidence="16" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="6" id="KW-0740">Sodium/potassium transport</keyword>
    <keyword evidence="7 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="53"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00025540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007791-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="21">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="323" mass="37683" checksum="BEC48957BA652B3F" modified="2020-08-12" version="1">MPKPVEEPFDQFQQYYRTPNNRSTAQSFQLFLWNSEEGAIFGRTPSSWSKIGIFYIVFYSVLAALVAVCMWVFFQTLDPRIPKWQMDQSLIGTNPGLGFRPLPSEDNVESTLIWYKGTDEKNYKMWTDALDDFLQDYRTPGQVSGRGQNIYNCDYNQPPPKGMVCDVDIKQYGPCTLENHYNYHKSAPCIFLKLNKIYGWVPEFYNESSSLPSNMPTDLKDYIKEKEQKELHTMNTVWVSCEGENAADIENVGQIQYYPRRGFPGYYYPYENSEGYLSPLVAVHFERPVRGIIINIECKAWAHNIKHDRHERMGTVHFELMID</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VTJ0</accession>
    <name>A0A6E8VTJ0_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ras-related protein Rab</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">The small GTPases Rab are key regulators in vesicle trafficking.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Membrane</location>
            <topology evidence="6">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040241171.1">
        <property type="nucleotide sequence ID" value="XM_040385237.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VTJ0"/>
    <dbReference type="EnsemblMetazoa" id="ACON007654-RA">
        <property type="protein sequence ID" value="ACON007654-PA"/>
        <property type="gene ID" value="ACON007654"/>
    </dbReference>
    <dbReference type="GeneID" id="120961499"/>
    <dbReference type="CTD" id="10981"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004658"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007654"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_029555"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045335">
        <property type="term" value="C:phagocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008333">
        <property type="term" value="P:endosome to lysosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090385">
        <property type="term" value="P:phagosome-lysosome fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04107">
        <property type="entry name" value="Rab32_Rab38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000222">
        <property type="entry name" value="RAB32, member RAS oncogene family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030697">
        <property type="entry name" value="Rab29/Rab38/Rab32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47981">
        <property type="entry name" value="RAB FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47981:SF39">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 6" id="KW-0636">Prenylation</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007654-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="231" mass="25897" checksum="8550E85E90DD9A1D" modified="2020-08-12" version="1">MVSSDGLHYAHPPKATMATGQAEKREHLYKILVIGELGTGKTSFIKRYVHQFFSQNYRATIGVDFALKVLNWDQNTIIRLQLWDIAGQERFGNMTRVYYKEAVGAFIVFDVTRSATFDAVIKWKNDLDSKVQLPDGKPIPCILLANKSDQQKQGIVTTPAKLDEYVKEHGFAGWFETSAKENVNIEEAAKSLVNKILMNDKLLNTGEVIDSERFALVGGKSDTSQKKSCSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VTJ7</accession>
    <name>A0A6E8VTJ7_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="4">EF-hand domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
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            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008538-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="661" mass="72165" checksum="784886E4552D1E73" modified="2020-08-12" version="1">TSTGCPPPDLKISRPRKQYATTEPPFVTFETTQIYLPQDFTTADFELVEAGKSGTNALRSHAETGGQSGSSSSSLHGGGGSHDTSFWWELGIQNAEVRESLAAASFPARSKRATVPSQRQRQHNHHHHHRRRHQTSADGLEAVELAEDGTAGGGGDQHQPGGGGRGQVRVKRKSGKATGALSRPKGASDSGSKSISRNANKGQYDEDGSYVPPLPDSAENPDREDKESEEVDESPELHQFREVSEVRFPGEVGPLGGHRLCKIQCIRGHWVGPLCAMNEHEQDEHGQLKFEPLYKRCVVDHIPPHLLLSYKNVSVTLGWDLPHGHTLQARCRDLGLYKLLGESKVLCSNGLWAPKMPSCVPTTLLTNYSDDSPPSIRIKVGVGSAAYEPSGVLAVLPTSTIHLDCMYPRRRGSPEWTWTGWFRQYLTGWSAVPEEKASRYRLTIKDIQNQDSGTYTCASPRGLTNSIVIVVAVSQCAALTEPNPPLSLRLEGIKLGQRALYRCPLGYMLQGTANATCLASGNWSSPTPTCHPIQCPPLFLEDPHLSLVELNTSAWGRAVFRCAWGYRLSGPPGLECEPNGHWSGPIPRCRGKAQCPYPGDPPNGLIAPLKFHYDPGDYLTVQCRPGFVEHGANGGPPERPRCTPEGDWSGPVPQCRSYEEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VVI9</accession>
    <name>A0A6E8VVI9_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_040236921.2">
        <property type="nucleotide sequence ID" value="XM_040380987.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040236922.2">
        <property type="nucleotide sequence ID" value="XM_040380988.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VVI9"/>
    <dbReference type="EnsemblMetazoa" id="ACON008515-RA">
        <property type="protein sequence ID" value="ACON008515-PA"/>
        <property type="gene ID" value="ACON008515"/>
    </dbReference>
    <dbReference type="GeneID" id="120958296"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012513"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008515"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031409"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050888">
        <property type="entry name" value="ZnF_C2H2-type_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24406">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR CTCFL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="296"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="545"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="609"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="42"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="364"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="427"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="565"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="626"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="62"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="364"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="498"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="569"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="580"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="665"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="686"/>
            <end position="698"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008515-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="698" mass="75911" checksum="8EDA1CEC891588C7" modified="2020-08-12" version="1">MASRTEEAFRAWLGRLRGLNSPAEAAKNVNVMLPLFRLDPDGGADSKKGHNASAAKDARSNPSPTVSTATPPASVSPPEKGIISYSDLKLGLLIKDQELLKLILKALRWAENDRRASFEVLIQRLKNSSFREILSNRNLLNDSDLTQLLKSYIGQGVMNSFAAMPPSAASTPLNNNNVVPPAVMFQPPINPTGGVKSGNQSGFVAPVGPIAGVNVNEYRIDEASVTQMEVGVDPDLYLPYEDEDSSSTNKFEGTRIEDRQENTVTIKLVTTNVAGDMIENKRMIPAILNVRGTKRFQCSACPECFETNGELQQHIVRRHLPRTNRTVITEQGKPAIKIRVRKNKPIAGPLLPPKLIPPSAPITLTATTPSPTVPTRDSGGVTVARSSSPIKIVPLKIPASTTITVIPATPPPATMVPVEPIKPPTVEQQVEVKPPPIKKEPSSAPASPAKGELLVRKSKRKTSLKPEVSSLILEKQQKKRKVQKVVQKPVLKEAQTPAPSPAPPAPPSPKPKTSRKSNAFCALCRKRLTPKQTMRVHMQQEHSRYECEKCDRAFKSALNLSRHQQYHASSSSSSSSSSSTVPTELVSSSASTKRAQKAAQQEEEKEQTHKCSYCEKAFKRSHHLKLHLKHHKKDATSAGSGLRRRAKLNQSSSSGSSGSAIKSVLVSSTAESGQGSKARKMEATKRKSTITRKVRFSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VVK6</accession>
    <name>A0A6E8VVK6_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Luc7 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VVK6"/>
    <dbReference type="EnsemblMetazoa" id="ACON008314-RC">
        <property type="protein sequence ID" value="ACON008314-PC"/>
        <property type="gene ID" value="ACON008314"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_008265"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008314"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032564"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006376">
        <property type="term" value="P:mRNA splice site recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004882">
        <property type="entry name" value="Luc7-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12375">
        <property type="entry name" value="RNA-BINDING PROTEIN LUC7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03194">
        <property type="entry name" value="LUC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008314-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="88" mass="10169" checksum="78DF6195D27F179C" modified="2020-08-12" version="1">MSAHDQMRAMLDQLMGTARNGETNRYSVKFYDSKVCKSFLLGCCPHEILASTTSVTTRRFFLTISSNKNHSQYKKRKIHTIAHTRQNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VVL5</accession>
    <name>A0A6E8VVL5_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="10">C2H2-type domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VVL5"/>
    <dbReference type="EnsemblMetazoa" id="ACON007973-RB">
        <property type="protein sequence ID" value="ACON007973-PB"/>
        <property type="gene ID" value="ACON007973"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000062"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007973"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040860"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048619">
        <property type="term" value="P:embryonic hindgut morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009880">
        <property type="term" value="P:embryonic pattern specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002571">
        <property type="entry name" value="Protein odd-skipped-related 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000311">
        <property type="entry name" value="protein odd-skipped-related 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050717">
        <property type="entry name" value="C2H2-ZF_Transcription_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14196">
        <property type="entry name" value="ODD-SKIPPED - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14196:SF11">
        <property type="entry name" value="PROTEIN SISTER OF ODD AND BOWEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="298"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="326"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="129"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="200"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="129"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="200"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007973-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="355" mass="36201" checksum="DB07538A5DA185A2" modified="2020-08-12" version="1">MLVEGIGAAVRNVSPAPENIPVPAVCALGMDQQSNESAAVAVAAAAAAAAAAAATGRSGGSGSQSAAGDFAAAAAVVLSLQNTMVTSLQQAALMPANSAAAAALNLQALESYLTLQRITSKSEVLRLSGSAAGHSTASHQRHQPLPSMPITGPAAQTTNDASGADGGDAIGDDMFMDTSGPSMLLQSNELADELPVLDQDEDLNFDDGPDDGSDGNSLDNGYPSLLLNAAFHQQMSADKMKTLAAAGSVGTAPSVSAATSASGAVGTKDLHSHAGAATAPTAAAVAAGGSGQRPKKQFICKFCNRQFTKSYNLLIHERTHTDERPYSCDICGKAFRRQDHLRDHRLQPHPNCRTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VVM3</accession>
    <name>A0A6E8VVM3_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="15">LRRCT domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VVM3"/>
    <dbReference type="EnsemblMetazoa" id="ACON008593-RB">
        <property type="protein sequence ID" value="ACON008593-PB"/>
        <property type="gene ID" value="ACON008593"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_013379"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008593"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040162"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034220">
        <property type="term" value="P:monoatomic ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001164">
        <property type="entry name" value="GH01279p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051432">
        <property type="entry name" value="KCNMA1_auxiliary"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46473">
        <property type="entry name" value="GH08155P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46473:SF10">
        <property type="entry name" value="LD45603P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 14" id="KW-0732">Signal</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026322673" evidence="14">
        <location>
            <begin position="38"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="529"/>
            <end position="550"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008593-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="590" mass="66930" checksum="6FD43E05F22249BC" modified="2020-08-12" version="1">MAHKTTLWRCHCWALVRTLLLSTLLAVVLWTAGEANALPYGAADIELDEANDAVSLTACYVEDLRNIREHSQNPLVIQINHCYLQELPNAIFIRFTDLQVLEICDSRVNNLQDFALNGLRNIESLNLSRNNLTTIKSWSDHDLDTLQVLDLRRNLIRSIDELSFQRYPGLVKLSLAVNFITTVPDGTFKPVANLKNLNLGKNLLTTIEEGTLRGLNKLTHVAFHHNRIRTIHPFAFVGNGHLKVLQLQGNQLGSFEPDLFGNLPRLTFLNVSSNELETVGNLSFKNSGDLRVLDLSYNRIGLLEDNSFKGLYDLEALNVSHNQLATVSKYVLKDCANLRDLDLSGNRLDYVGLKLSSATPRLVRLNLSRNAIGEIERDVFEDLPKLHTLDLAHNQLTDDAFLWSLMNLSVLNMSQNGFRKINASLLESLTLAELYDNPWDCRFLVQELAHHSSSVIYGKNYLVEDTGRILTATGIECTDERGKHRDIVVVEPPAKSSPDEMEFQRYKFFHEGHVDTRPIQDNFDTKSTVIWLMSGAIAVFGAFKLIQLLLRHSEHQSEKWRLAQHLEYNLPDDRDIAISKAMEANRMDDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VVM4</accession>
    <name>A0A6E8VVM4_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="14">ANK_REP_REGION domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON008559-RA">
        <property type="protein sequence ID" value="ACON008559-PA"/>
        <property type="gene ID" value="ACON008559"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_013917"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008559"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042880"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034703">
        <property type="term" value="C:cation channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000241">
        <property type="entry name" value="No mechanoreceptor potential C, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000329">
        <property type="entry name" value="No mechanoreceptor potential C, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050889">
        <property type="entry name" value="Dendritic_Spine_Reg/Scaffold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002153">
        <property type="entry name" value="TRPC_channel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24166:SF48">
        <property type="entry name" value="PROTEIN VAPYRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24166">
        <property type="entry name" value="ROLLING PEBBLES, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01097">
        <property type="entry name" value="TRNSRECEPTRP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="27"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="19"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 10" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="9" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
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</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VVN1</accession>
    <name>A0A6E8VVN1_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glycine--tRNA ligase</fullName>
            <ecNumber evidence="6">6.1.1.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Diadenosine tetraphosphate synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">Glycyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="25 26">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="25">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="21">Catalyzes the ATP-dependent ligation of glycine to the 3'-end of its cognate tRNA, via the formation of an aminoacyl-adenylate intermediate (Gly-AMP). Also produces diadenosine tetraphosphate (Ap4A), a universal pleiotropic signaling molecule needed for cell regulation pathways, by direct condensation of 2 ATPs. Thereby, may play a special role in Ap4A homeostasis. Required for terminal arborization of both dendrites and axons during development.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>2 ATP + H(+) = P(1),P(4)-bis(5'-adenosyl) tetraphosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:34935"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58141"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:34936"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>tRNA(Gly) + glycine + ATP = glycyl-tRNA(Gly) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:16013"/>
            <dbReference type="Rhea" id="RHEA-COMP:9664"/>
            <dbReference type="Rhea" id="RHEA-COMP:9683"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78522"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.14"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:16014"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Axon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.14" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8VVN1"/>
    <dbReference type="EnsemblMetazoa" id="ACON008604-RA">
        <property type="protein sequence ID" value="ACON008604-PA"/>
        <property type="gene ID" value="ACON008604"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000575"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008604"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040064"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:glycine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
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        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:mitochondrial glycyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="GlyRS-like_core"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="GlyRS_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GlyRS_RNA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Glycine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Glycine--tRNA ligase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Glycine--tRNA ligase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.10:FF:000007">
        <property type="entry name" value="Glycyl-tRNA synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Glycyl-tRNA synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Glycyl-tRNA synthetase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.720.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.10">
        <property type="entry name" value="S15/NS1, RNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027031">
        <property type="entry name" value="Gly-tRNA_synthase/POLG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033731">
        <property type="entry name" value="GlyRS-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002315">
        <property type="entry name" value="tRNA-synt_gly"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009068">
        <property type="entry name" value="uS15_NS1_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000738">
        <property type="entry name" value="WHEP-TRS_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00389">
        <property type="entry name" value="glyS_dimeric"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003211">
        <property type="entry name" value="PRK04173.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10745:SF0">
        <property type="entry name" value="GLYCINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10745">
        <property type="entry name" value="GLYCYL-TRNA SYNTHETASE/DNA POLYMERASE SUBUNIT GAMMA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00458">
        <property type="entry name" value="WHEP-TRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01043">
        <property type="entry name" value="TRNASYNTHGLY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00991">
        <property type="entry name" value="WHEP-TRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47060">
        <property type="entry name" value="S15/NS1 RNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00762">
        <property type="entry name" value="WHEP_TRS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51185">
        <property type="entry name" value="WHEP_TRS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="17" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0436">Ligase</keyword>
    <keyword evidence="15" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <keyword evidence="14" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="WHEP-TRS" evidence="24">
        <location>
            <begin position="9"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="23">
        <location>
            <begin position="66"/>
            <end position="559"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00019404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00030057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00049523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00055201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00078924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS51185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008604-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="679" mass="75766" checksum="07C0509FF424B148" modified="2020-08-12" version="1">MTDPKIEAELAPLREAVKEQGDLVRKLKSEGAPEIDVKKAVNELKARKKVLEDRELSLAPQVASFDRARMEDLLKRRFFYDQSFAIYGGITGQYDFGPMGCALKSNMINTWRQFFVLEEQMLEVDCSILTPEPVLKASGHVDRFADLMTKDVKNGECFRLDHLIKNHLEKLAAAKDATAELKDECADIVIKLDGMTKDEMAAILRKYNMKSPITGNDLTEPIEFNLMFGTQIGPTGLVKGFLRPETAQGIFVNFKRLLEFNQGRLPFAAAQIGNSFRNEISPRSGLIRVREFTMCEIEHFCDPQAKNHPKFENVADTVMTLYSACNQMDGKSAQQIRIGDAVASGLVANETLGYFMARIQMYLHRIGILPERLRFRQHMGNEMAHYACDCWDAECLTSYGWIECVGCADRSAYDLTQHTNATGVKLVAEKKLPAPKTIEVTEVVPNKAAIGKAFKKEAKAITEALAKLSLGEVETIGKSLGDSGEHLLAVNGTDVKLTNDLIAVKTTSKTVHVEEITPSVIEPSFGIGRIMYSLLEHSFRMREGDEQRSYFSLPPVVAPLKCSVLPLSNNAEFAPFVKKISSALTSVDVSHKVDDSSGSIGRRYARTDEIAIPYGITIDFDTLKEPHTVTLRERDSMKQVRIGLDEVANTIRDLATGRTSWAEVEQKYPRFEQQEASAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VXP5</accession>
    <name>A0A6E8VXP5_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">CRAL-TRIO domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VXP5"/>
    <dbReference type="EnsemblMetazoa" id="ACON009385-RA">
        <property type="protein sequence ID" value="ACON009385-PA"/>
        <property type="gene ID" value="ACON009385"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007853"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON009385"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037394"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902936">
        <property type="term" value="F:phosphatidylinositol bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.20">
        <property type="entry name" value="N-terminal domain of phosphatidylinositol transfer protein sec14p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011074">
        <property type="entry name" value="CRAL/TRIO_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10174">
        <property type="entry name" value="ALPHA-TOCOPHEROL TRANSFER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10174:SF212">
        <property type="entry name" value="MIP26555P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00180">
        <property type="entry name" value="CRETINALDHBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM01100">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="1">
        <location>
            <begin position="98"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009385-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="229" mass="26607" checksum="D5F821EF465E628D" modified="2020-08-12" version="1">MSLKPFDIEVDAPGDELMEIARRELRETPEMRAQAVEELRALLREAKDMHFSEEEAFLLIFLRPCHFYAESALKMMRRIAEFKKTNHPLLHALKPEEEKLAFVEHNVVNVLTNRDQKGRRVLLVNCGAAWDPKAVSSEQLFRVFFLIHLVAQLEPATQINGVVVVLDFDGLSLKQVKALSPSFSKRLITFIQDAMPLRLKEVHILKQPYIFNMVWALFKPFIREKLKSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VXQ0</accession>
    <name>A0A6E8VXQ0_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Hemolymph juvenile hormone binding protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the TO family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VXQ0"/>
    <dbReference type="EnsemblMetazoa" id="ACON009388-RB">
        <property type="protein sequence ID" value="ACON009388-PB"/>
        <property type="gene ID" value="ACON009388"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011626"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON009388"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033340"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007623">
        <property type="term" value="P:circadian rhythm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.15.10.30:FF:000001">
        <property type="entry name" value="Takeout-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.15.10.30">
        <property type="entry name" value="Haemolymph juvenile hormone binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010562">
        <property type="entry name" value="Haemolymph_juvenile_hormone-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038606">
        <property type="entry name" value="To_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11008:SF39">
        <property type="entry name" value="CIRCADIAN CLOCK-CONTROLLED PROTEIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11008">
        <property type="entry name" value="PROTEIN TAKEOUT-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06585">
        <property type="entry name" value="JHBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00700">
        <property type="entry name" value="JHBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00060902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009388-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="262" mass="29487" checksum="B37ED4A3AEE42C9A" modified="2020-08-12" version="1">MQQPAWRCNRAAQSVALAAVIVLLAVHYSGAVFPTVLRACPRNSTDLNQCIIEVVNELRPRLATGDFGENFTITSLEPIKIDRLTIERGENFKANFTNFRISGATHEKTGFIVKKLKTDIDNRQINATILLPKLQVNSKYSLKMSILVLQINGDGDLQVNLTDTKVSLKLTFYTETVDGEEYFRFNPINLRVKFGKARFYLKNLFNGDPTLEMIGNQAINENPDVLLDEVKGGIEENLAKLFTKIASEVVKDALFEEVFPWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VXS2</accession>
    <name>A0A6E8VXS2_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">1-acyl-sn-glycerol-3-phosphate acyltransferase</fullName>
            <ecNumber evidence="5">2.3.1.51</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 1-acyl-sn-glycero-3-phosphate + an acyl-CoA = a 1,2-diacyl-sn-glycero-3-phosphate + CoA</text>
            <dbReference type="Rhea" id="RHEA:19709"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57970"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="EC" id="2.3.1.51"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 2/3.</text>
    </comment>
    <comment type="domain">
        <text evidence="5">The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.51" evidence="5"/>
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        <property type="nucleotide sequence ID" value="XM_040379029.2"/>
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        <property type="nucleotide sequence ID" value="XM_040379030.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040234965.1">
        <property type="nucleotide sequence ID" value="XM_040379031.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040234966.1">
        <property type="nucleotide sequence ID" value="XM_040379032.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_049466497.1">
        <property type="nucleotide sequence ID" value="XM_049610540.1"/>
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    <dbReference type="AlphaFoldDB" id="A0A6E8VXS2"/>
    <dbReference type="EnsemblMetazoa" id="ACON009415-RA">
        <property type="protein sequence ID" value="ACON009415-PA"/>
        <property type="gene ID" value="ACON009415"/>
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    <dbReference type="VEuPathDB" id="VectorBase:ACON2_029564"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003841">
        <property type="term" value="F:1-acylglycerol-3-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006654">
        <property type="term" value="P:phosphatidic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="LPLAT_AGPAT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AGP_acyltrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00530">
        <property type="entry name" value="AGP_acyltrn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10434">
        <property type="entry name" value="1-ACYL-SN-GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10434:SF53">
        <property type="entry name" value="1-ACYL-SN-GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="5" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="61"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="91"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="124"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="7">
        <location>
            <begin position="91"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009415-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="273" mass="30806" checksum="5F2FDE0747F160A0" modified="2020-08-12" version="1">MTTSNGELLGLAFMAFFIITLSSTARYYFKFFCFIAFSVVCAVGPVPLMLFRPRDYRNALIPAYLCIGFGKMLGASFTVRGKENINRNHGGIVLMNHQSALDLIVLAELWPIMGRSTVVAKKEVLYMFPFGLACWLWGTLFIDRQNRDSAKSVINKEAKAINEKQAKLLFFPEGTRGNGDKLLPFKKGCFHVAVESQAFIQPVVISKYHFLKSKAKIFNRGQNIIKILPEVSCAGLSKDDIPALMERVQKMMQREYEQLSEESLSINNISEVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VY10</accession>
    <name>A0A6E8VY10_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VY10"/>
    <dbReference type="EnsemblMetazoa" id="ACON009477-RA">
        <property type="protein sequence ID" value="ACON009477-PA"/>
        <property type="gene ID" value="ACON009477"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004055"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON009477"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_036021"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12640">
        <property type="entry name" value="RRM3_Bruno_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CUGBP Elav-like family member 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000322">
        <property type="entry name" value="CUGBP Elav-like family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24012">
        <property type="entry name" value="RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
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        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="266"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009477-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="351" mass="36865" checksum="E60A2AA44E70C89C" modified="2020-08-12" version="1">MLSKKYNENDVRQLFSGHGTIEECTVLRDPSGQSKGCAFVTFATKQAAIGAIKQLHQSKTMEGCSAPLVVKFADTQKEKDAKRMQQMQTNLWGMTGINQPITQSVTPLTASAHPNPPQQQTPFLAADAISPPLSLLQQLQAVAQQQHLLQGLSAQTDTTSAAAASLLAPISLQNLVTLAGITQPSLQTAAAAAAQTPATAISNAATSLWTTGADTATGLTSYAALPQFGAAAATAAAFTPTPLTTPTLAQAVAAAAGKQIEGPEGCNLFIYHLPQEFTDTDLASTFLPFGNVVSAKVFIDKQTNLSKCFGFVSFDNVSSAQAAIQAMHGFQIGTKRLKVQLKRSKDASKPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VY17</accession>
    <name>A0A6E8VY17_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">RB1-inducible coiled-coil protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">FAK family kinase-interacting protein of 200 kDa</fullName>
        </alternativeName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="23">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Involved in autophagy. Regulates early events but also late events of autophagosome formation through direct interaction with Atg16L1. Required for the formation of the autophagosome-like double-membrane structure that surrounds the Salmonella-containing vacuole (SCV) during S.typhimurium infection and subsequent xenophagy. Involved in repair of DNA damage caused by ionizing radiation, which subsequently improves cell survival by decreasing apoptosis. Inhibits PTK2/FAK1 and PTK2B/PYK2 kinase activity, affecting their downstream signaling pathways. Plays a role as a modulator of TGF-beta-signaling by restricting substrate specificity of RNF111. Functions as a DNA-binding transcription factor. Is a potent regulator of the RB1 pathway through induction of RB1 expression. Plays a crucial role in muscular differentiation. Plays an indispensable role in fetal hematopoiesis and in the regulation of neuronal homeostasis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Lysosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Preautophagosomal structure</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_040239583.2">
        <property type="nucleotide sequence ID" value="XM_040383649.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040239584.2">
        <property type="nucleotide sequence ID" value="XM_040383650.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040239585.2">
        <property type="nucleotide sequence ID" value="XM_040383651.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040239586.2">
        <property type="nucleotide sequence ID" value="XM_040383652.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040239587.2">
        <property type="nucleotide sequence ID" value="XM_040383653.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040239588.2">
        <property type="nucleotide sequence ID" value="XM_040383654.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_049465367.1">
        <property type="nucleotide sequence ID" value="XM_049609410.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_049465368.1">
        <property type="nucleotide sequence ID" value="XM_049609411.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_049465369.1">
        <property type="nucleotide sequence ID" value="XM_049609412.1"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON008873-RB">
        <property type="protein sequence ID" value="ACON008873-PB"/>
        <property type="gene ID" value="ACON008873"/>
    </dbReference>
    <dbReference type="GeneID" id="120959901"/>
    <dbReference type="KEGG" id="acoz:120959901"/>
    <dbReference type="CTD" id="40700"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_003652"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008873"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039187"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990316">
        <property type="term" value="C:Atg1/ULK1 kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031090">
        <property type="term" value="C:organelle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
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        <property type="term" value="F:protein kinase binding"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:autophagosome assembly"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:autophagy of mitochondrion"/>
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        <property type="term" value="P:glycophagy"/>
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        <property type="term" value="P:negative regulation of cell population proliferation"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:reticulophagy"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:ribophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Ubl_RB1CC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000049">
        <property type="entry name" value="RB1-inducible coiled-coil protein 1 isoform X1"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR045326">
        <property type="entry name" value="ATG17-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13222">
        <property type="entry name" value="RB1-INDUCIBLE COILED-COIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13222:SF1">
        <property type="entry name" value="RB1-INDUCIBLE COILED-COIL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="9" id="KW-0072">Autophagy</keyword>
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    <keyword evidence="11 19" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0458">Lysosome</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Autophagy protein ATG17-like" evidence="21">
        <location>
            <begin position="117"/>
            <end position="550"/>
        </location>
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    <feature type="domain" description="Autophagy-related protein 11 C-terminal" evidence="22">
        <location>
            <begin position="1061"/>
            <end position="1186"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="220"/>
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        </location>
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    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
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            <end position="1485"/>
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            <begin position="830"/>
            <end position="886"/>
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        <location>
            <begin position="997"/>
            <end position="1093"/>
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        <location>
            <begin position="239"/>
            <end position="249"/>
        </location>
    </feature>
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        <location>
            <begin position="630"/>
            <end position="640"/>
        </location>
    </feature>
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        <location>
            <begin position="641"/>
            <end position="656"/>
        </location>
    </feature>
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        <location>
            <begin position="1198"/>
            <end position="1220"/>
        </location>
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        <location>
            <begin position="1260"/>
            <end position="1271"/>
        </location>
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        <location>
            <begin position="1337"/>
            <end position="1352"/>
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            <begin position="1401"/>
            <end position="1416"/>
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            <begin position="1417"/>
            <end position="1434"/>
        </location>
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</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VY39</accession>
    <name>A0A6E8VY39_ANOCL</name>
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        <submittedName>
            <fullName evidence="3">N-myc downstream regulated</fullName>
        </submittedName>
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        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NDRG family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VY39"/>
    <dbReference type="EnsemblMetazoa" id="ACON008821-RD">
        <property type="protein sequence ID" value="ACON008821-PD"/>
        <property type="gene ID" value="ACON008821"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011297"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008821"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041407"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="entry name" value="protein NDRG3 isoform X8"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AB_hydrolase_fold"/>
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    </dbReference>
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        <property type="entry name" value="N-MYC DOWNSTREAM REGULATED"/>
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    </dbReference>
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        <property type="entry name" value="Ndr"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
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            <begin position="18"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="EnsemblMetazoa" id="ACON008821-PD"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="419" mass="45740" checksum="75E41EC659C3C12F" modified="2020-08-12" version="1">MPQSDGGYVSLGGGGPNSIYNSTTEPQSGSKSVMETVKRVIGTAIGAQDKHALLERDGTGAQPYIVSARDRDKTGKSANARMPSAPAHTAEEARLLGAMPVDPMDDIELRSVQLQYPNQRGSIVGACDLRRVPTDKGDILVAVQGDTTKPAIVTYHDLGLNYASSFAGFFNFPTMRSLLDNFCVYHVNAPGQEDGAPTFPEDYVYPTFDELASQMLFVMTHFNLKSIIGFGVGAGANILARFALANPDKVGALCLINCSSTAAGWIEWGYQLLNTRNLRTKGMTQGVLDYLMWHHFGRNPEERNLDLVQLYKSNFERSINPVNLAMLIDAYIKRTDLNIARTPSGSPQTSAPSLKMPVLNITGALSPHIDDTVTFNGRLIPEKTNWMKISDCGLVLEEQPGKLAEAFRLFLQGEGYEKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VZR1</accession>
    <name>A0A6E8VZR1_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="6">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the PPP phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8VZR1"/>
    <dbReference type="EnsemblMetazoa" id="ACON010172-RA">
        <property type="protein sequence ID" value="ACON010172-PA"/>
        <property type="gene ID" value="ACON010172"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_014212"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON010172"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033524"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07415">
        <property type="entry name" value="MPP_PP2A_PP4_PP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000003">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047129">
        <property type="entry name" value="PPA2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45619">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE PP2A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0464">Manganese</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="7">
        <location>
            <begin position="80"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010172-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="275" mass="31517" checksum="6BA442DD8C6AEBAE" modified="2020-08-12" version="1">AKEILSKESNVQEVKCPVTVCGDVHGQFHDLMELFRIGGRSPDTNYLFMGDYVDRGYYSVETVTLLVALKVRYRERITILRGNHESRQITQVYGFYDECLRKYGNPNVWKFFTDLFDYLPLTALVDGQIFCLHGGLSPSIDTLDHIRALDRLQEVPHEGPMCDLLWSDPDDRGGWGISPRGAGYTFGQDISELFNHTNGLTLVSRAHQLVMEGYNWCHDRNVVTIFSAPNYCYRCGNQAALMELDDALKFSFLQFDPAPRRGEPHVTRRTPDYFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VZR6</accession>
    <name>A0A6E8VZR6_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Adenylyl cyclase-associated protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CAP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040238155.1">
        <property type="nucleotide sequence ID" value="XM_040382221.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VZR6"/>
    <dbReference type="EnsemblMetazoa" id="ACON010175-RA">
        <property type="protein sequence ID" value="ACON010175-PA"/>
        <property type="gene ID" value="ACON010175"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON010175-RB">
        <property type="protein sequence ID" value="ACON010175-PB"/>
        <property type="gene ID" value="ACON010175"/>
    </dbReference>
    <dbReference type="GeneID" id="120959067"/>
    <dbReference type="CTD" id="45233"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000190"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON010175"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042252"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008179">
        <property type="term" value="F:adenylate cyclase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019933">
        <property type="term" value="P:cAMP-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.330:FF:000001">
        <property type="entry name" value="Adenylyl cyclase-associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.70:FF:000001">
        <property type="entry name" value="Adenylyl cyclase-associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.330">
        <property type="entry name" value="Adenylate cyclase-associated CAP, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001837">
        <property type="entry name" value="Adenylate_cyclase-assoc_CAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013912">
        <property type="entry name" value="Adenylate_cyclase-assoc_CAP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017901">
        <property type="entry name" value="C-CAP_CF_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016098">
        <property type="entry name" value="CAP/MinC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036223">
        <property type="entry name" value="CAP_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028417">
        <property type="entry name" value="CAP_CS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053950">
        <property type="entry name" value="CAP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036222">
        <property type="entry name" value="CAP_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006599">
        <property type="entry name" value="CARP_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003124">
        <property type="entry name" value="WH2_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10652">
        <property type="entry name" value="ADENYLYL CYCLASE-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10652:SF0">
        <property type="entry name" value="ADENYLYL CYCLASE-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08603">
        <property type="entry name" value="CAP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21938">
        <property type="entry name" value="CAP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00673">
        <property type="entry name" value="CARP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69340">
        <property type="entry name" value="C-terminal domain of adenylylcyclase associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101278">
        <property type="entry name" value="N-terminal domain of adenylylcyclase associated protein, CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51329">
        <property type="entry name" value="C_CAP_COFACTOR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01089">
        <property type="entry name" value="CAP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51082">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WH2" evidence="6">
        <location>
            <begin position="208"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="C-CAP/cofactor C-like" evidence="7">
        <location>
            <begin position="263"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="168"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="179"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010175-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="429" mass="46219" checksum="5267AED1D90F657C" modified="2020-08-12" version="1">MSINAYEDIMLGSFANFIALSNKIGGDVATQAQFVKEAFDAQFAFLKVASASSAPSNTDLQNLLKPTSDKISTIQSYREKNRTSPFFNHLSAISESIPALGWVCVAPTPGPYVKEMNDAGQFYTNRVLKDWKEKDSTHVEWARAWIQTLTELQQYIKQHHTTGLVWSGKDKPTVGGASVPPPPPPGGLPPPPPMMPLGDLSADGSGDDRSALFAQINQGADITKGLKKVTADMQTHKNPSLRSGPAPYKAPAGVTNGTKSVAPPAAAAVAKPPTFTRDGKKWLIEYQKNNPDLVVENAEMNNVVYMFRCEGSTLQIKGKINSVVMDSCKKCSLVFDSLVASAEFVNCQSVQMQVLGKVPTISIDKTDGCQMYLSADSLAVEIVSSKSSEMNVMIPKGSSGDYTEQPIPEQFKTIVKGSSLNTTCVESLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VZT4</accession>
    <name>A0A6E8VZT4_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="12">ABC transporter domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VZT4"/>
    <dbReference type="EnsemblMetazoa" id="ACON009466-RB">
        <property type="protein sequence ID" value="ACON009466-PB"/>
        <property type="gene ID" value="ACON009466"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011440"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON009466"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034839"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03213">
        <property type="entry name" value="ABCG_EPDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001077">
        <property type="entry name" value="Uncharacterized protein, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050352">
        <property type="entry name" value="ABCG_transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041:SF133">
        <property type="entry name" value="GH24286P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="344"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="383"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="412"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="442"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="493"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="74"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009466-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="523" mass="58111" checksum="280DEDCCAA318818" modified="2020-08-12" version="1">MAEESYEMNPASSAELNGGPADPEAGTSPVSGVGGRRAGAAKLEPLQIPAVPAVEQEIAAPSLIAARLFKPVTVSFKNLSYSVRNGIFRKGKTRRDILKDISGEFRAGELTAIMGPSGAGKSTLLDILAGFTEGGFTGELLVNKQPRDLKRFRRMSAYIMQDHDLQPHLTVLEAMHFSANLKIGAELSPASKKIRMNEILRAIGLHEAKKTRTGRLSGGQKKRLAIALEIVNNPPVMFFDEPTSGLDSSTSTQCVALLKQLAREGRTIICTIHQPSALLFNMFDHLYAVAEGECIYTGGTGNIVSFLKELDIVCPEHYNPSDYFPLELPIITREHFNRWYSLRAYYIAMTVADIPIQLLCTVTYILITYYMTGQPPEAFRVGLFTLICLMVAWVAQGLGLLVASLFDVKNGAIFGPFFICPFLIFSGFFIHLTDAHPIMHWLFHISFLKYALQGAALAIFGYDRPRMDCDETYCHFVLPKKFLKEVDMLEADFVEAVIALGVIFFVFRVSAFYVMQYRMKNKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VZU3</accession>
    <name>A0A6E8VZU3_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Gustatory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Gustatory receptor which mediates acceptance or avoidance behavior, depending on its substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the insect chemoreceptor superfamily. Gustatory receptor (GR) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VZU3"/>
    <dbReference type="EnsemblMetazoa" id="ACON009805-RE">
        <property type="protein sequence ID" value="ACON009805-PE"/>
        <property type="gene ID" value="ACON009805"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACON009805"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034541"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007635">
        <property type="term" value="P:chemosensory behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008049">
        <property type="term" value="P:male courtship behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050909">
        <property type="term" value="P:sensory perception of taste"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013604">
        <property type="entry name" value="7TM_chemorcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21143:SF134">
        <property type="entry name" value="GUSTATORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21143">
        <property type="entry name" value="INVERTEBRATE GUSTATORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08395">
        <property type="entry name" value="7tm_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="39"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="69"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="133"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="164"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="275"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="353"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009805-PE"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="396" mass="45968" checksum="689625616530BD91" modified="2020-08-12" version="1">MSNLLKRYRLLLAVASIGYLIPCSYNIRTGLFDCSYRNTLVCVCNVVFFGGFVWYDFGMILKFYATLPIVLVGILTVDVTVYNLLIFCIIINAVYNRDCFVQLLNSLFARDDWMLESVAMQGSSQQRRSTQSTGGLVCLIVLVLLYAMYNALFVNDHSMVLMDMIILLRFCFMFLILELYRVCVRIIRKRMKQLQVLLTQMEEINTTASVEHVVHVFLDRFQRYYLLIDSVNKCFSVPVTHTLLLIVLERTVAAYDVFENLRGESKMILWDFYRLLYRQVWEITYIVLMVLLAINCNATSLQVEETALCTRHFDDYRLQNTRAAKQIQKFLLKNLHQKKKFSACGFFDIDNTVIYMVFSSIVTYLVILIQFKQLETDLTQAGDGYNVTSNVSTVQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VZU4</accession>
    <name>A0A6E8VZU4_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="8">TLDc domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Synapse</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VZU4"/>
    <dbReference type="EnsemblMetazoa" id="ACON010235-RA">
        <property type="protein sequence ID" value="ACON010235-PA"/>
        <property type="gene ID" value="ACON010235"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_008814"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON010235"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034857"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006571">
        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354:SF122">
        <property type="entry name" value="GTPASE-ACTIVATING PROTEIN SKYWALKER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354">
        <property type="entry name" value="NUCLEOLAR PROTEIN 7/ESTROGEN RECEPTOR COACTIVATOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07534">
        <property type="entry name" value="TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00584">
        <property type="entry name" value="TLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51886">
        <property type="entry name" value="TLDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0770">Synapse</keyword>
    <feature type="domain" description="TLDc" evidence="7">
        <location>
            <begin position="398"/>
            <end position="574"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010235-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="576" mass="65429" checksum="5B0D0824DEDCD24E" modified="2020-08-12" version="1">MPVNAIGAEQLSGIEEESDIYEAFAPHVDTSNISVLTDTPPGKKPNLKSFQDVQSLIQQGKKREAKIVLRENSWPTNSPIRSQLWPALCAQHHVGKTMLDGFYWDMVNQVFGTTELPEKPIMLPPFVDSTHCLPYHLTRKGRAVADRVVSVLGYACPDITYSPTLYPITSILLHFMSEEECYHCMASLVAPKEKVFITQTKLLYEVTWKTVMQIAKKHAKSAVNYLSTMCNGQKPESIFMDWCWWILGGLPFPHLVRVMDCFFHEGIKVFYRVSLAILILFHKHVTSSNSEWDTDAIKNDIDNALPKFCKNIPVSPAKLLRTAFSIRALSSTYISRVFLKTEMLLKSKSVLSGPKQMVRSRSSDNLPTSQSQINVQMMSHTLTIREVSVMCCIKRRSTVIKKCLFTLWSWLPVRITMYQPILLYTTEEHGCSLTTFYVRVEQHEPTLLMIKTCNNEVFGAYCSSRWFERNLKDDRGQRQAYFGTGETFLFSLYPERAKYPWVGIEGDTGLGHASELFMAADSKMITIGGGEGQAIWMDENIRFGKTDRCQTFNNPPLCASGDFEIRVLEVYGFVGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VZU8</accession>
    <name>A0A6E8VZU8_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Gustatory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Gustatory receptor which mediates acceptance or avoidance behavior, depending on its substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the insect chemoreceptor superfamily. Gustatory receptor (GR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VZU8"/>
    <dbReference type="EnsemblMetazoa" id="ACON009805-RJ">
        <property type="protein sequence ID" value="ACON009805-PJ"/>
        <property type="gene ID" value="ACON009805"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACON009805"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007635">
        <property type="term" value="P:chemosensory behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008049">
        <property type="term" value="P:male courtship behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050909">
        <property type="term" value="P:sensory perception of taste"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013604">
        <property type="entry name" value="7TM_chemorcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21143:SF134">
        <property type="entry name" value="GUSTATORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21143">
        <property type="entry name" value="INVERTEBRATE GUSTATORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08395">
        <property type="entry name" value="7tm_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="40"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="73"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="136"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="163"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="356"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009805-PJ"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="399" mass="46478" checksum="F1107AB4210A467A" modified="2020-08-12" version="1">MKWVQEILNNLSTIGVVLVRYDGEQGSYRRPTTHQCVTHFLLVLFSIGFLLYCIWEQYSIFAMAIYAFDDATFILSTASFIVAWFVVPLVSCARGDRLIYTLNNLLENDTDLSRLHAIQSSVRMSYRKAKWFGRMIQYESIACCLLLSAGYTILYSFQPEAWYLYLTIGIYLYEDLCLSWLFGFYSTIMMHCVDQLSYAAQLLQEDHAHTLDERVRVFCKTYERVCRAVARSFFEYTGPVIATFCTLVMFEGSLKLYHVYDVFIRGVNEIVWTEHMLNVIECLWLCFDVKKLFIVMYISGMLQQKVEETALCTRHFDDYRLQNTRAAKQIQKFLLKNLHQKKKFSACGFFDIDNTVIYMVFSSIVTYLVILIQFKQLETDLTQAGDGYNVTSNVSTVQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VZU9</accession>
    <name>A0A6E8VZU9_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="3">J domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VZU9"/>
    <dbReference type="EnsemblMetazoa" id="ACON010239-RB">
        <property type="protein sequence ID" value="ACON010239-PB"/>
        <property type="gene ID" value="ACON010239"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005385"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON010239"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038781"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10747">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.260.20:FF:000002">
        <property type="entry name" value="Dnaj homolog subfamily b member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000033">
        <property type="entry name" value="dnaJ homolog subfamily B member 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.260.20:FF:000026">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.260.20">
        <property type="entry name" value="Urease metallochaperone UreE, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002939">
        <property type="entry name" value="DnaJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051339">
        <property type="entry name" value="DnaJ_subfamily_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008971">
        <property type="entry name" value="HSP40/DnaJ_pept-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24078:SF553">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY B MEMBER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24078">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01556">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49493">
        <property type="entry name" value="HSP40/DnaJ peptide-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="2">
        <location>
            <begin position="4"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010239-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="345" mass="39190" checksum="B82F0F903E43E43C" modified="2020-08-12" version="1">MGKDYYKTLGIPRGSTDEDIKKAYRKLALKYHPDKNKSPGAEEKFKEVAEAYEVLSDKKKREMYDKFGEDGLKGPSNGTSNSSQNFTYEFHGDPRATFAQFFGSNNPFGSFFDMHNDSLFNSSIFNDDDFFTPFSGLGNRHGLGGAFRSHSFNVHSPLKKEKVQDPPIEHDLYVTLEEIYHGCVKKMKISRRVLQPDGTSKKEDKCVSISIKPGWKSGTKVTFQKEGDQTKGKIPADIVFIIRDKPHVWFRREGSDLRYTARLTLKQALCGVIFEVPTMTGEKLRISTKQEIIKPNTVKRIQGYGLPFPKEPSRKGDLLVAFDIKFPDKLSTSEKEMLNDMLPNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W1B0</accession>
    <name>A0A6E8W1B0_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Calponin-homology (CH) domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8W1B0"/>
    <dbReference type="EnsemblMetazoa" id="ACON010012-RB">
        <property type="protein sequence ID" value="ACON010012-PB"/>
        <property type="gene ID" value="ACON010012"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005836"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON010012"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033213"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21205">
        <property type="entry name" value="CH_LRCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000081">
        <property type="entry name" value="Leucine-rich-repeats and calponin homology domain protein, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF21">
        <property type="entry name" value="CALPONIN-HOMOLOGY (CH) DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="4">
        <location>
            <begin position="698"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="377"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="439"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="622"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="829"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="377"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="449"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="461"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="493"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="557"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="574"/>
            <end position="590"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010012-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="869" mass="93268" checksum="70BD22CC53667D8E" modified="2020-08-12" version="1">MAVAACLKATQSTVVYSGQHQPLHLVGMNGVGNVGIAGGHLQSQLTRSLERILEDAHLSGELKLSGRKLKDFPKAAGKYNLSDTVIADLSRNRFCELPEDITCLAFLERLLVYHNTIRSVPETIRGLHSLTYLDLRNNQLSVLPREICALPLQVLLVSNNRLATLPDELGRMEKLTELDAACNQITHLPARMGDLRNMRSLNLRSNQLVYLPRDLTCLELAFLDISSNKIATLPVELRHMTSLVDLELSNNPLTSPPASLCVRGLVHVFKYLETVASREEKSKTGIDGHATLRRTALSAKNSSSCLLDSQQRNRRANVDSGYCTSDGGFDGGQHVALATHPNHGGPHESTVDTAVAGTTGGSNDVCWEEEFRKNATFQEHHPDRKQLSNNNSNDISPEGDITPDGTKTDDKGRTLSNYQTYREYKEALRQQRNLDSVYRSKDHPLTPDSAGSATDSPVSNVSPYTKSISSSSVYSSSSQSSPVSPHHTIGVQKMNQINANPNSSYHLSGATGTHSSPLLSPNRNGSQGTTVDDSTGGTPNKRPVQKVIPSRNIGSLHHNQSPTHLNGNHHASGAASTSVSNGSHGKNALSNGAGKGSLSSANGSVNNEYAYVKPNSPCKSTSGVLAHNNIPPSSIPKPTAPNSGGLGSPGATQKPLTATVGYVNNAKPGQKANKTVSWNRDVPTEKLSFTMRREFDKQKEETELIEQLRQIIETRLKMSLPQDIAPALMDGVVLCHLANLVRPRSVGSIHVPSSAVPKLTMARCRRNVDYFLDACRKIGVDENLLCCAADVLEGRGIVQVAITVVELLKFHNPASNVRSPTRSMYALANNLNSSGNSGSSGSSSSSSSSVQKSSPSTSSLSSPVGGNGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W3C0</accession>
    <name>A0A6E8W3C0_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Kinesin-like protein unc-104</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Required for presynaptic maturation, has a role in axonal transport of dense-core vesicles carrying synaptic vesicle precursors, components required for the morphological transformation of axonal growth cones to mature boutons.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="SMR" id="A0A6E8W3C0"/>
    <dbReference type="EnsemblMetazoa" id="ACON010519-RH">
        <property type="protein sequence ID" value="ACON010519-PH"/>
        <property type="gene ID" value="ACON010519"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001094"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON010519"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037311"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904115">
        <property type="term" value="C:axon cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048490">
        <property type="term" value="P:anterograde synaptic vesicle transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021700">
        <property type="term" value="P:developmental maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051222">
        <property type="term" value="P:positive regulation of protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040012">
        <property type="term" value="P:regulation of locomotion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051960">
        <property type="term" value="P:regulation of nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010975">
        <property type="term" value="P:regulation of neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008582">
        <property type="term" value="P:regulation of synaptic assembly at neuromuscular junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd22705">
        <property type="entry name" value="FHA_KIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01365">
        <property type="entry name" value="KISc_KIF1A_KIF1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01233">
        <property type="entry name" value="PH_KIFIA_KIFIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.20:FF:000001">
        <property type="entry name" value="Kinesin family member 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000004">
        <property type="entry name" value="Kinesin-like protein isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000204">
        <property type="entry name" value="kinesin-like protein unc-104 isoform X6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2520">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022164">
        <property type="entry name" value="Kinesin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022140">
        <property type="entry name" value="Kinesin-like_KIF1-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032405">
        <property type="entry name" value="Kinesin_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049780">
        <property type="entry name" value="PH_KIFIA_KIFIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47117:SF10">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIF1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47117">
        <property type="entry name" value="STAR-RELATED LIPID TRANSFER PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12473">
        <property type="entry name" value="DUF3694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12423">
        <property type="entry name" value="KIF1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16183">
        <property type="entry name" value="Kinesin_assoc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="9 12" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="16">
        <location>
            <begin position="3"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="15">
        <location>
            <begin position="1542"/>
            <end position="1640"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="391"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="952"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="359"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="617"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="398"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="968"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="97"/>
            <end position="104"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00056070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010519-PH"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1644" mass="185650" checksum="8E5EEB528BC0B1DD" modified="2020-08-12" version="1">MSSVKVAVRVRPFNSREIARESKCIIEMAGNTTCITNPKVPPGSSESVKRFNYDYSYWSHDPRDLEFSTQAMVYSDIGEEMLQHSFDGYNVCIFAYGQTGAGKSYTMMGKQEDGQEGVIPMICKDLFRRIQETESDDLKYSVEVSYMEIYCERVRDLLNPKNKGNLKVREHPLLGPYVEDLSKLAVTSYQDIHDLIDEGNKARTVAATNMNETSSRSHAVFTIFFTQKRQDRMTSLETEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEIASKNKKSKKADFIPYRDSVLTWLLRENLGGNSKTAMIAAISPADINYDETLSTLRYADRAKQIVCKAVVNEDANAKLIRELKEEIQKLRELLKAEGIEVQEGEDGEKKIHSPNRNRKRTGSSTEMAVDQLQASEKLIAELNETWEEKLKRTEQIRVQREAVFAEMGVAVKEDGITVGVFSPKKSPHLVNLNEDPTLSECLLYYIKDGLTRLGTSEANVPQDIQLSGSHILKEHCVFENKDGVVTLVPHKDALVYVNGRKVIEPEVLQTGSRVILGRNHVFRFTHPEQAREKREKNKEVDVCETPGNSGEIADWNFAQCELLEKQGIDLKAEMEKRLLALEEQYKREKRAADQEFEEQRKTYEARIDALQKQVEEQSMTMSMYSSYSPEDFHQEEDIFVNPLFESCWTAREAGLAAWAFRKWRYHQFTSLRDDLWGNAIFLKEANAISVELKKKVQFQFTLLTDTLYSPLPPELTPAPAVGALTNGGQEDEFGQNPIPRTTVAVEVTDTKNGATHYWSLEKLRQRLELMREMYHNEAELSPTSPDYNVESLTGGDPFYDRFPWFRMVGRSFVYLSNLLYPVPLVHKVAIVNERGDVRGYLRVAVQPVMDEENADFSNGVKQSARILFDEEQNGQHKVPKIRTIPDKDEKYIEGGNEMGTKLEELEQEDADSGRGDSSVASELHESNDQEPGEHLQPGKEFTFRVTVLQATGIAAEYADIFCQFNFLHRHEEAFSTEPVKNSGSGAPLGFYHVQNITVPVTKSFIEYLKTQPIVFKVFGHYQHHPLHKDAKQDCQITRPPPRRMLPPSIPISQPVRSPKFGPLPCPPSSTVLAKHDVLVWFEICELAPNGEYVPAVVDHSDDLPCRGLFLLHQGIQRRIRITIVHEPTPEVKWKDIRELVVGRIRNQPEPADDEDSDSCVLSLGLFPGEVLEVPGDDRSFFRFEAAWDSSLHNSALLNRVTQTGEQIYITLSAYLELDNCARPAIITKDLSMIIYGRDARTGPRSLKHLFSGQYRNPEANRLSGVYELSLRRASEAGSPGVQRRQRRVLDTSSTYVRGEENLHGWRPRGDSLIFDHQWELEKLTRLEEVGRVRHLLLLRERLGMDTTPNPTTKTEKDVCNLAQRASASPVHMVIPPSPQTPVKDQQAPAMPERELSVRETELVWKCVKLIQGRIGCKELGAEGNGSGTQASDASPGDEGCADMNASYISSNSIELCSPERVDMPNGWEAPAPAPQTQDLSLRLYVPELEEIRVSPVVARKGYLNVLEHGGSGWKKRWVTVRRPYVFIFRSDKDPVERAVLNLATAQVECSEDQAAMVKVPNTFSVVTKHRGYLLQTLGDKEVHDWLYAINPLLAGQIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W3F3</accession>
    <name>A0A6E8W3F3_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">diacylglycerol cholinephosphotransferase</fullName>
            <ecNumber evidence="13">2.7.8.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>1,2-dioctanoyl-sn-glycerol + CDP-choline = 1,2-dioctanoyl-sn-glycero-3-phosphocholine + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54232"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58779"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:76979"/>
            <dbReference type="ChEBI" id="CHEBI:78228"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:54233"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>1-hexadecanoyl-2-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycerol + CDP-choline = 1-hexadecanoyl-2-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycero-3-phosphocholine + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54332"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58779"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:74963"/>
            <dbReference type="ChEBI" id="CHEBI:82949"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:54333"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycerol + CDP-choline = 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54244"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58779"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:73001"/>
            <dbReference type="ChEBI" id="CHEBI:75466"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:54245"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>CDP-choline + a 1,2-diacyl-sn-glycerol = a 1,2-diacyl-sn-glycero-3-phosphocholine + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32939"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58779"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="2.7.8.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:32940"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="12">Phospholipid metabolism; phosphatidylcholine biosynthesis; phosphatidylcholine from phosphocholine: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.2" evidence="13"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8W3F3"/>
    <dbReference type="EnsemblMetazoa" id="ACON011766-RA">
        <property type="protein sequence ID" value="ACON011766-PA"/>
        <property type="gene ID" value="ACON011766"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005668"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON011766"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_029329"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004142">
        <property type="term" value="F:diacylglycerol cholinephosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004307">
        <property type="term" value="F:ethanolaminephosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006646">
        <property type="term" value="P:phosphatidylethanolamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1760:FF:000002">
        <property type="entry name" value="Choline/ethanolamine phosphotransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048254">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014472">
        <property type="entry name" value="CHOPT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10414:SF37">
        <property type="entry name" value="BB IN A BOXCAR, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10414">
        <property type="entry name" value="ETHANOLAMINEPHOSPHOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="47"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="147"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="185"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="215"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="254"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="290"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="325"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="423"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="432"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="452"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="474"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00036100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00036651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00036890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON011766-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="488" mass="54993" checksum="ECB840036E34D929" modified="2020-08-12" version="1">MYFYKQKLLHAAQLKKLYDHKYSCTNVSLMDPFLQPWWCWLVSKVPLWLAPNLITIVGLAINIATTLILISYSPTGREEPPRWSSALCGIGLFIYQSLDAIDGKQARRTNSSSPLGELFDHGCDSISTVFVALSACISVQLGYYPRWMFFQCFCAMTLFYCAHWQTYVSGTLRFGRIDVTEAQCTIIGIHMISAIFGPSIWMTKIFGQFELWSTMAVMTILCGGWSLLEFFSVIRAGGVGKNGSTVAMPLLGGSWNYAVCAIFTFGFVQVFTTFCQSFRAGGVGKNGSTVAGTSVLSPIIPFLFVVVPAYVISQKSTDQIYENHPALYIMAFGMITAKVTNRLVVAHMTKSEMEYLDWGLIGPLCLFLNQYFNSFLPEYYVLWFCLLWYTVDLIRYCGQVCLEICDYLKIELFRIPYPPKPVMTSQGTNPPQHHHHHHHHSHDNKNGTHSLTNNNNSAATSAQQQQQQQSINRKQTRASSRKHNAAAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W5G8</accession>
    <name>A0A6E8W5G8_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 7">Trehalase</fullName>
            <ecNumber evidence="3 7">3.2.1.28</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Alpha-trehalose glucohydrolase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>alpha,alpha-trehalose + H2O = alpha-D-glucose + beta-D-glucose</text>
            <dbReference type="Rhea" id="RHEA:32675"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15903"/>
            <dbReference type="ChEBI" id="CHEBI:16551"/>
            <dbReference type="ChEBI" id="CHEBI:17925"/>
            <dbReference type="EC" id="3.2.1.28"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the glycosyl hydrolase 37 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.28" evidence="3 7"/>
    <dbReference type="RefSeq" id="XP_040231065.2">
        <property type="nucleotide sequence ID" value="XM_040375131.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8W5G8"/>
    <dbReference type="EnsemblMetazoa" id="ACON012053-RA">
        <property type="protein sequence ID" value="ACON012053-PA"/>
        <property type="gene ID" value="ACON012053"/>
    </dbReference>
    <dbReference type="GeneID" id="120954857"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004924"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON012053"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031903"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004555">
        <property type="term" value="F:alpha,alpha-trehalase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005993">
        <property type="term" value="P:trehalose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001661">
        <property type="entry name" value="Glyco_hydro_37"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018232">
        <property type="entry name" value="Glyco_hydro_37_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23403">
        <property type="entry name" value="TREHALASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23403:SF1">
        <property type="entry name" value="TREHALASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01204">
        <property type="entry name" value="Trehalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00744">
        <property type="entry name" value="GLHYDRLASE37"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00927">
        <property type="entry name" value="TREHALASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00928">
        <property type="entry name" value="TREHALASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="560"/>
            <end position="581"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON012053-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="582" mass="66597" checksum="7992D73E9524DD7B" modified="2020-08-12" version="1">MLVASKPGPIQRLLDSEIYCHGELLKTVQMSEIYPDSKTFVDMKMRKSPNETLDSFHEFMVAQDNSPSKAKLKEWVESNFEKPGAEFENWTPDDWTASPKFLARIKDEDLRGFASELNKIWHSLGRKMTSDVALNPDLYSIIHVDHPVIVPGGRFREFYYWDSYWIVKGLLLSEMYSTTKGMLENFLSIIQRYGFIPNGGRIYYSMRSQPPLLCGMVKSYVDATNDTKFAMDSVETLEREFQFFMNNYVTEVNNHQLATYGYKSSGPRPESYREDVLSAAIFQREEDKQDYYCELKAAAESGMDFSSRWFIKDGTNAGNLTDLKCRSIVAVELNAILYWNALIIAEFYGYRNDIGSIAKKEEYLAKADELKKAINAVLWDEAEGAWFDYDLINKKLRKYFTPTNLSPLWVGCYAREDKELPKRILAYIDRLHLDRYPGGVPNTLQNTNEQWDFPNVWAPMQHMLVMGLDSLDNAEAKELAFSWAQRWVRGNYLTFNKTHSMFEKYDAQELGGHGSGGEYEVQTGFGWTNGAAMDLMNKYADRLTTVDAAEQPEDNGNGALVAYSINTGIIATLLAFFVGVFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W9M5</accession>
    <name>A0A6E8W9M5_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON029051-RB">
        <property type="protein sequence ID" value="ACON029051-PB"/>
        <property type="gene ID" value="ACON029051"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007811"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON029051"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040800"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030056">
        <property type="term" value="C:hemidesmosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005882">
        <property type="term" value="C:intermediate filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045104">
        <property type="term" value="P:intermediate filament cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042060">
        <property type="term" value="P:wound healing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00176">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000260">
        <property type="entry name" value="Kakapo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000275">
        <property type="entry name" value="Kakapo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000001">
        <property type="entry name" value="Microtubule-actin cross-linking factor 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1290.10:FF:000014">
        <property type="entry name" value="microtubule-actin cross-linking factor 1 isoform X21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000110">
        <property type="entry name" value="Short stop, isoform AA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000031">
        <property type="entry name" value="Short stop, isoform J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000044">
        <property type="entry name" value="Short stop, isoform K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000045">
        <property type="entry name" value="Short stop, isoform K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000051">
        <property type="entry name" value="Short stop, isoform K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000054">
        <property type="entry name" value="Short stop, isoform K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000058">
        <property type="entry name" value="Short stop, isoform K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000063">
        <property type="entry name" value="Short stop, isoform K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000068">
        <property type="entry name" value="Short stop, isoform K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000082">
        <property type="entry name" value="Short stop, isoform K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000038">
        <property type="entry name" value="Short stop, isoform N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000039">
        <property type="entry name" value="Short stop, isoform N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000040">
        <property type="entry name" value="Short stop, isoform N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000042">
        <property type="entry name" value="Short stop, isoform N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000047">
        <property type="entry name" value="Short stop, isoform N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000050">
        <property type="entry name" value="Short stop, isoform N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000057">
        <property type="entry name" value="Short stop, isoform N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="24"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.920.20">
        <property type="entry name" value="Gas2-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1290.10">
        <property type="entry name" value="Plakin repeat"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003108">
        <property type="entry name" value="GAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036534">
        <property type="entry name" value="GAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043197">
        <property type="entry name" value="Plakin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035915">
        <property type="entry name" value="Plakin_repeat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001101">
        <property type="entry name" value="Plectin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018159">
        <property type="entry name" value="Spectrin/alpha-actinin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002017">
        <property type="entry name" value="Spectrin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23169">
        <property type="entry name" value="ENVOPLAKIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23169:SF23">
        <property type="entry name" value="SHORT STOP, ISOFORM H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02187">
        <property type="entry name" value="GAS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00435">
        <property type="entry name" value="Spectrin"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00243">
        <property type="entry name" value="GAS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00250">
        <property type="entry name" value="PLEC"/>
        <property type="match status" value="31"/>
    </dbReference>
    <dbReference type="SMART" id="SM00150">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="28"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143575">
        <property type="entry name" value="GAS2 domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75399">
        <property type="entry name" value="Plakin repeat"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46966">
        <property type="entry name" value="Spectrin repeat"/>
        <property type="match status" value="25"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51460">
        <property type="entry name" value="GAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="7007"/>
            <end position="7042"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
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            <dbReference type="Google" id="ProtNLM"/>
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</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W9S6</accession>
    <name>A0A6E8W9S6_ANOCL</name>
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        <recommendedName>
            <fullName evidence="15">UPAR/Ly6 domain-containing protein qvr</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Protein quiver</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Protein sleepless</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Bifunctional regulator of neuronal activity in the mushroom body, and possibly other regions of the brain, that acts as a signaling molecule required for homeostatic regulation of sleep under normal conditions and after sleep deprivation. Reduces neuronal excitability by enhancing Sh/shaker K(+) channel activity; possibly by stabilizing Sh/shaker to increase protein levels, accelerating its activation kinetics, slowing C-type inactivation and enhancing recovery from inactivation. Specifically affects the A-type K(+) current. Antagonizes nicotinic acetylcholine receptors (nAChRs) to reduce synaptic transmission, possibly by preventing their localization to the cell surface. Required for regulation of neuromuscular excitability and plasticity at neuromuscular junctions.</text>
    </comment>
    <comment type="subunit">
        <text evidence="18">Interacts (via loop 2 of the three-fingered Ly-6 domain) with Sh/shaker; this interaction may stabilize both components of the complex and may be required for targeting or retention of Sh/shaker to neural cell projections. Interacts (via loop 2 of the three-fingered Ly-6 domain) with nAChRalpha3 and potentially other nicotinic acetylcholine receptors; this interaction is required for antagonism of nicotinic acetylcholine receptors.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
            <orientation evidence="1">Extracellular side</orientation>
        </subcellularLocation>
        <subcellularLocation>
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            <topology evidence="14">Lipid-anchor</topology>
            <topology evidence="14">GPI-anchor</topology>
            <orientation evidence="14">Extracellular side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the quiver family.</text>
    </comment>
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        <property type="protein sequence ID" value="ACON028190-PA"/>
        <property type="gene ID" value="ACON028190"/>
    </dbReference>
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    <dbReference type="VEuPathDB" id="VectorBase:ACON028190"/>
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        <property type="component" value="Unplaced"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:regulation of synaptic transmission, cholinergic"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:rhythmic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:sleep"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="TFP_LU_ECD_Qvr"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="QVR-like"/>
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        <property type="entry name" value="Sleep_regulator"/>
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        <property type="entry name" value="ATILLA, ISOFORM B-RELATED-RELATED"/>
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        <property type="entry name" value="PROTEIN QUIVER"/>
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    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
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        <location>
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    </feature>
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    </evidence>
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            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
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        <source>
            <dbReference type="EnsemblMetazoa" id="ACON028190-PA"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="156" mass="17869" checksum="F671CF048775CF50" modified="2020-08-12" version="1">MDRKQWVIIPLVILISSVYLCSADNCFPSRSIYCYECDSWTDARCKDPFNYTALPRDQPPLMTCNGCCVKMVRHSRTPYEVVRRMCTSQLQINLFMVDHVCMMESSGNGHMCFCEEDMCNSSPSSLLTANPHHTVHVCIGSVLAVYLLQLLQRVFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WBA1</accession>
    <name>A0A6E8WBA1_ANOCL</name>
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        <submittedName>
            <fullName evidence="9">Protein kinase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_040235495.2">
        <property type="nucleotide sequence ID" value="XM_040379561.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8WBA1"/>
    <dbReference type="EnsemblMetazoa" id="ACON029628-RA">
        <property type="protein sequence ID" value="ACON029628-PA"/>
        <property type="gene ID" value="ACON029628"/>
    </dbReference>
    <dbReference type="GeneID" id="120957371"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005638"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON029628"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034953"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043065">
        <property type="term" value="P:positive regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14103">
        <property type="entry name" value="STKc_MLCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000534">
        <property type="entry name" value="Myosin light chain kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000594">
        <property type="entry name" value="Myosin light chain kinase isoform-III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24342:SF20">
        <property type="entry name" value="MYOSIN LIGHT CHAIN KINASE, SMOOTH MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24342">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="34"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="373"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="579"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="637"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="379"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="397"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="418"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="595"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="638"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON029628-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="805" mass="88609" checksum="C78033272F405DF9" modified="2023-06-28" version="2">MIHVDETDPLGDIPPAFPYREVEIKRGIDPKQIYELSSELGRGKFGVVYKCKEKSTGVRLAAKFIQIVKKGDRRNIEREVHMMNVLHHSKIAQLYAAYEFDRTFCVMMELVEGGELFDRVLDEKFVLTEKACSIFMRQICDAVAYIHGNNIIHLDMKPENILCLTESGNRIKIIDFGLAREYDPDNKLQVLFGTPEFVAPEVVNFEAISFATDMWSVGVIAYVLVSGLSPFAGEDDIQTMGNITIGRYDFLDEAFDNVSEEAIDFINRCLVKEQKERLTAEDALKHKWIKRKPQYHPTNRRPSLTNSFKPILLESNNNSSNVTNAEIAKDNLKELVGKWDETPNNRYAFEQDTENIIAPSGETVQLRRPTLPELAGLANGSNTSGSRRGSIARDSDSVASSCDTTASVCTDPAPSPTTPLHLLHPGSPAQPNPPRTVGSAGDGDAVTDTHRDKALDGVEQSALNASTVTTPTTPAPALELDSELESLKSKVNTIRTSRAQNKLNELAQRPDFLANDPFKLPTFRFLPKSISLCNEDSVFKENYTKFCDRNASGRLSREQSQETTVSEKIDTVGTIVRKKSTTSSTTKKKKDPEGAATATTTTTTTTTFSSTPSGKIASTTTVRKTIVKQQKIVVEDASSSVTTVGTSESSRSRSIAPTKSLDVSSSSTALDSSKLTHSASIKRTKFRVNQLSSRDVPVAINSVARRYLERSGIQDDLSTSAARLPRTLSPTGPGQTTLDDTVTVNNLRNSALRLRSSVSVDWDAMEAVENRSMKTINTFLKRHAVASSAVKQIQAQLEATITTHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WBD6</accession>
    <name>A0A6E8WBD6_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phosphoinositide phospholipase C</fullName>
            <ecNumber evidence="5">3.1.4.11</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + H2O = 1D-myo-inositol 1,4,5-trisphosphate + a 1,2-diacyl-sn-glycerol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:33179"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
            <dbReference type="ChEBI" id="CHEBI:203600"/>
            <dbReference type="EC" id="3.1.4.11"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.1.4.11" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8WBD6"/>
    <dbReference type="EnsemblMetazoa" id="ACON029661-RC">
        <property type="protein sequence ID" value="ACON029661-PC"/>
        <property type="gene ID" value="ACON029661"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007025"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON029661"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042366"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032587">
        <property type="term" value="C:ruffle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004435">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate phospholipase C activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048468">
        <property type="term" value="P:cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046488">
        <property type="term" value="P:phosphatidylinositol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010634">
        <property type="term" value="P:positive regulation of epithelial cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051209">
        <property type="term" value="P:release of sequestered calcium ion into cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09932">
        <property type="entry name" value="SH2_C-SH2_PLC_gamma_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11825">
        <property type="entry name" value="SH3_PLCgamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000119">
        <property type="entry name" value="1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.190:FF:000043">
        <property type="entry name" value="1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000009">
        <property type="entry name" value="1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001192">
        <property type="entry name" value="PI-PLC_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035023">
        <property type="entry name" value="PLC-gamma_C-SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001711">
        <property type="entry name" value="PLipase_C_Pinositol-sp_Y"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336:SF159">
        <property type="entry name" value="1-PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE PHOSPHODIESTERASE GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336">
        <property type="entry name" value="PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE C FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00387">
        <property type="entry name" value="PI-PLC-Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00390">
        <property type="entry name" value="PHPHLIPASEC"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00149">
        <property type="entry name" value="PLCYc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50008">
        <property type="entry name" value="PIPLC_Y_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="1 4" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH2" evidence="7">
        <location>
            <begin position="45"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="8">
        <location>
            <begin position="164"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="PI-PLC Y-box" evidence="9">
        <location>
            <begin position="325"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="298"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON029661-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="502" mass="57866" checksum="3CF36A276460BC1D" modified="2020-08-12" version="1">MENVLFESLYSLIMYYRQNALRSAEFYITLKEPVPQPNKHETKEWYHQTTTREQSEIVLNQIPQDGAFLVRPSEKGPKAFVISFRAHGKFKHCRIRVEGRLYEMGGMEFESLVDLVNFYSKHPLYRKVTLTYPIPREMIRRINTMDVPEESGGYGYMDPATVTNENVTVKALYDYKAQRDDELSFCKHAIITNVVKRGNDWWLGDYGGKRQHYFPANYVQELSAMDDGTLVEEGANEPLMLGSLQKGSLDVHGAVVEMAYSNHPDIERILRIQNPTMQNVFEVGVQTKELAYEWMCAIKEAAQNASAMENERRKMERNSRVAKEMSDLIIYCRSVPFKHHPTAWVFYEMSSFPETKAEKYFLQAETRALFVRYHRNHLSRVYPKGQRLDSSNYHPTALWNCGSQMIALNFQTPDKPMQLNQAKFRDNGGCGYLLKPEFMFRDEFDPTDPNTLVGVGEVIVNVRIIGGRNLCKVSRNITSPLVEVEVLGASFDGGIKHRTRAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WBD9</accession>
    <name>A0A6E8WBD9_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Tetraspanin</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8WBD9"/>
    <dbReference type="EnsemblMetazoa" id="ACON027982-RB">
        <property type="protein sequence ID" value="ACON027982-PB"/>
        <property type="gene ID" value="ACON027982"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001485"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON027982"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03155">
        <property type="entry name" value="CD151_like_LEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1450.10">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008952">
        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF544">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00259">
        <property type="entry name" value="TMFOUR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48652">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="96"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="228"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON027982-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="261" mass="29580" checksum="F777550D7DD657C0" modified="2020-08-12" version="1">MGHLLQPDDTSEIELFEPMNLIYTGSTTTGKTSKMGRRTDENPTIVSNTELGFNNGQSTFRSRKTKKTINPKHKTRDSDCCSVNFIKYSLHIFNTIFLYTFVLLIVFLLEIIVGSLAYLYETQIETELQHTLNATFMEHYGVSEQQTKAIDSMQQEFSCCGAVRFEDWRHSVWLRSRRKNLIKPTEGRLVPDSCCITVVSNCGLRDGPSNIHYTGCIYGMMDDLKYHLIILGAIGLGLSAIQVFGMVLSCCLYVKLKDVLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9I3A836</accession>
    <name>A0A9I3A836_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Diacylglycerol kinase</fullName>
            <shortName evidence="13">DAG kinase</shortName>
            <ecNumber evidence="13">2.7.1.107</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2023-03" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>a 1,2-diacyl-sn-glycerol + ATP = a 1,2-diacyl-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10272"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.107"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the eukaryotic diacylglycerol kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.107" evidence="13"/>
    <dbReference type="AlphaFoldDB" id="A0A9I3A836"/>
    <dbReference type="EnsemblMetazoa" id="ACON000519-RB">
        <property type="protein sequence ID" value="ACON000519-PB"/>
        <property type="gene ID" value="ACON000519"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034782"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004143">
        <property type="term" value="F:ATP-dependent diacylglycerol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010646">
        <property type="term" value="P:regulation of cell communication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0023051">
        <property type="term" value="P:regulation of signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043052">
        <property type="term" value="P:thermotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd20802">
        <property type="entry name" value="C1_DGK_typeIV_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20855">
        <property type="entry name" value="C1_DGK_typeIV_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000204">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.40:FF:000012">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10330:FF:000020">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037607">
        <property type="entry name" value="DGK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056383">
        <property type="entry name" value="DGKI-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000756">
        <property type="entry name" value="Diacylglycerol_kin_accessory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001206">
        <property type="entry name" value="Diacylglycerol_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11255">
        <property type="entry name" value="DIACYLGLYCEROL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11255:SF80">
        <property type="entry name" value="EYE-SPECIFIC DIACYLGLYCEROL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00609">
        <property type="entry name" value="DAGK_acc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00781">
        <property type="entry name" value="DAGK_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23578">
        <property type="entry name" value="DGKI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00045">
        <property type="entry name" value="DAGKa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00046">
        <property type="entry name" value="DAGKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50146">
        <property type="entry name" value="DAGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="10 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="15" id="KW-0732">Signal</keyword>
    <keyword evidence="3 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039893500" description="Diacylglycerol kinase" evidence="15">
        <location>
            <begin position="25"/>
            <end position="1072"/>
        </location>
    </feature>
    <feature type="domain" description="DAGKc" evidence="16">
        <location>
            <begin position="371"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="12">
        <location>
            <begin position="967"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="12">
        <location>
            <begin position="1003"/>
            <end position="1035"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="817"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="878"/>
            <end position="890"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000519-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1072" mass="117716" checksum="19E74434BE2BB040" modified="2023-06-28" version="1">MVLQSTASLLCMACVARVAHLSRARVRPKRQLGRLLHRTRDRLARMSSRCGCCCGWPGGTARARRRSHCPPTYGRSASYPGRRCSFGTFGAPGGAAGGRYRRRSVAAGGLAVRKISASGRYAQPWPVGEGAFGLGSSWPGRRRAFRVRDRSLVALRMRRRGDPHGRQIRCTPDWSENAVSGDHLWVPTSVSGDCCCVGDNECTHGPRMKCAACKIIAHASCISVLMERSHLQCKPTFRDVGVRQYREQTKTTHHWVHRRTEKGKCKQCGKSLQAKLTFSSKEIVALSCAWCKASYHNKEACFNPERIGEECTLGTHRSIVVPPSWIVKLPRKGNFKSSLRKTPQKKKSKAGKKVSIPREPRTFVIKPIPTANLTPVLVFINPKSGGNQGAKLLQKFQWLLNPRQVFDLTQGGPRMGLELFRKVPQLRILACGGDGTVGWVLSVLDQINFVPPPAVGVLPLGTGNDLARALGWGGGYTDEPIGKILANIGNSDTVLLDRWSLKVEPNTSVPNTGDGKDNLPLNVVNNYFSLGVDAHIALEFHEAREAHPEKFNSRLRNKMFYGQAGGKDLLKRKWKGLAEFVTLECDGKDLTPKLKEHKVHAIVFLNIPSYGGGTHPWNKSGGQFEPATDDGMIEVVGLTTYQLPLLQAGGHGTCITQCRTARIVTSKTIPMQVDGEACKLNPSNIELTLLNKAVMLAKKKPGRANVPQEKLESLHMSLMKIMMADYEQHHYDKELLKQSAVNLGSLEVPVTDLEQVRVLINKYCGEQPDSPKLSPDWCFIDSCTAERFFRVDRAQENLHFITDIANDCVFVLDQESPTLPQTPEDEHRRLGAHGTPVAGSLESGDGGAPCSPQAGKLLDRSLSGPPAATASDIPSRKGSQGSQDADGQQARPPIGHDSVGNGQSLDQMVNFKSFHERLFGLNEDAFGFSNLLEKTTDAVIKASKTGDLVMLKDLHLQGYSLLSIDSTGQTALHHGARCGHKDIVRYLISYAPNSIINMIDNETGQTALHKAAAGRQRSICYMLVAGGANLNIQDNDGRTARMLASNADDFELAFYLENQEQFQLIDFRSNNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9I3AJX4</accession>
    <name>A0A9I3AJX4_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="6">EF-hand domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2023-03" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A9I3AJX4"/>
    <dbReference type="EnsemblMetazoa" id="ACON004631-RC">
        <property type="protein sequence ID" value="ACON004631-PC"/>
        <property type="gene ID" value="ACON004631"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_036458"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052110">
        <property type="entry name" value="MCFD2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23104:SF17">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23104">
        <property type="entry name" value="MULTIPLE COAGULATION FACTOR DEFICIENCY PROTEIN 2 NEURAL STEM CELL DERIVED NEURONAL SURVIVAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039931682" evidence="5">
        <location>
            <begin position="22"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="32"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="46"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004631-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="167" mass="19810" checksum="5641386C0D59BBA7" modified="2023-06-28" version="1">MKHSVLITLAILMQLASLAICQRVAPGVNPNHYQQQQQYQPPPPQYQQQAPQHQQPQYQQQPPQQQQQPQYQQQQFQQQQQHQQPHGEHGQQQVLHTGNLQQEKQHIAEHMEVPIDTSKMSEQELQFHYFKMHDSDNNNKLDGCELIKSLIHWHDRYWILRSYLTAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABM2BF16</accession>
    <name>A0ABM2BF16_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON030774.R3">
        <property type="protein sequence ID" value="ACON030774.P3"/>
        <property type="gene ID" value="ACON030774"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052253">
        <property type="entry name" value="CR1/CR2-DNA-binding_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13006:SF9">
        <property type="entry name" value="GLUCOSE TRANSPORTER 4 ENHANCER FACTOR, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13006">
        <property type="entry name" value="PAPILLOMAVIRUS REGULATORY FACTOR PRF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01366">
        <property type="entry name" value="c-clamp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="32"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="75"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="163"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="304"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="121"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="95"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="169"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="214"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="231"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON030774.P3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="368" mass="39754" checksum="320C1BD391880655" modified="2025-10-08" version="1">MASGQVLMSRSCVARRTQVFKPKVRNVSQRIFKCTWKGCGVIYTTCTEITQHVRNAHLGPRKPNEYSDEEDFFFTETEDESDESAPNPPSPPTLSHRDMARPPHEDPEYQRQIVGTYRQGLLSQSQALQQQQQQQQAAQQQQQQHQAQQQQIASIKPPLPAGLTVAALSGSNGTSSASNGGSNGSSSSSNSNGSTSNGAVNPHSSVSPLIHHNYTWSQVSPTSPQKHVRLSPRPSASYPYPSPNYQHHYQAQAAQQQQQHHQQQLGHIQLSPGAGSGSNGTAAYHQLLAGAAGATGAITLGSYSSSPGSSGGGSSVGSAGSGQHRSPVSPNRRTRGENKKCRKVYGMDHKDQWCTQCKWKKACSRFGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A182KUW3</accession>
    <name>A0A182KUW3_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_040234289.1">
        <property type="nucleotide sequence ID" value="XM_040378355.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A182KUW3"/>
    <dbReference type="STRING" id="1518534.A0A182KUW3"/>
    <dbReference type="EnsemblMetazoa" id="ACOM027377-RA">
        <property type="protein sequence ID" value="ACOM027377-PA"/>
        <property type="gene ID" value="ACOM027377"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON010640-RA">
        <property type="protein sequence ID" value="ACON010640-PA"/>
        <property type="gene ID" value="ACON010640"/>
    </dbReference>
    <dbReference type="GeneID" id="120956684"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001064"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACOM027377"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON010640"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042857"/>
    <dbReference type="Proteomes" id="UP000075882">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="127"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="74"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010640-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="185" mass="19428" checksum="67E578CC06D4D22B" modified="2016-09-07" version="1">MFSGITASLKNLGSKAEGLFDKKKKEAQDLANEKVQAASSMAEEQAKKTQESFSKTKSDAEALASSAAGEIDATKQQAAAAAETTSQAAGTLMDHAKQAAENAIAQSAVAVEQAVEEQMKVAEQKVDEGMKRASEEVDRKLQEANRVADEKRGELEQKVNDVATKAQESATASAAGLLGKLNLGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8UXK5</accession>
    <name>A0A6E8UXK5_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Neurobeachin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON000017-RC">
        <property type="protein sequence ID" value="ACON000017-PC"/>
        <property type="gene ID" value="ACON000017"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009738"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000017"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032851"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06071">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01201">
        <property type="entry name" value="PH_BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1540.10:FF:000001">
        <property type="entry name" value="neurobeachin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000059">
        <property type="entry name" value="neurobeachin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000010">
        <property type="entry name" value="neurobeachin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1540.10">
        <property type="entry name" value="BEACH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000409">
        <property type="entry name" value="BEACH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036372">
        <property type="entry name" value="BEACH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050865">
        <property type="entry name" value="BEACH_Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046851">
        <property type="entry name" value="NBCH_WD40"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010508">
        <property type="entry name" value="NBEA-like_DUF1088"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031570">
        <property type="entry name" value="NBEA/BDCP_DUF4704"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046852">
        <property type="entry name" value="Neurobeachin_a-sol"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023362">
        <property type="entry name" value="PH-BEACH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13743">
        <property type="entry name" value="BEIGE/BEACH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13743:SF162">
        <property type="entry name" value="NEUROBEACHIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02138">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06469">
        <property type="entry name" value="DUF1088"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15787">
        <property type="entry name" value="DUF4704"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20426">
        <property type="entry name" value="NBCH_WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20425">
        <property type="entry name" value="Neurobeachin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14844">
        <property type="entry name" value="PH_BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01026">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81837">
        <property type="entry name" value="BEACH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50197">
        <property type="entry name" value="BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51783">
        <property type="entry name" value="PH_BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="BEACH-type PH" evidence="8">
        <location>
            <begin position="2397"/>
            <end position="2505"/>
        </location>
    </feature>
    <feature type="domain" description="BEACH" evidence="7">
        <location>
            <begin position="2524"/>
            <end position="2813"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="3109"/>
            <end position="3140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1096"/>
            <end position="1197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1211"/>
            <end position="1239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1259"/>
            <end position="1322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1384"/>
            <end position="1431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1447"/>
            <end position="1609"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1910"/>
            <end position="1985"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="2318"/>
            <end position="2360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="1126"/>
            <end position="1162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1180"/>
            <end position="1195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1289"/>
            <end position="1308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1392"/>
            <end position="1401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="1404"/>
            <end position="1424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="1457"/>
            <end position="1496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1508"/>
            <end position="1522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1523"/>
            <end position="1549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1554"/>
            <end position="1572"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1910"/>
            <end position="1921"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1932"/>
            <end position="1951"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1959"/>
            <end position="1971"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="2321"/>
            <end position="2338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000017-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="3197" mass="353725" checksum="08CD66BB24949099" modified="2020-08-12" version="1">MADLTRPPLCDIKRPEEVVKMSMTDNLKFAVLIGLIEVGQVSNREVVNTVLHLLVGGEFDMELNFVVQDAQNVKHMLELLDHCPANLQAEVWSVFIAILKKSVRNLQACTEIGLIEHVLARLQQAEPIVADLLIEMLGVLASYSITVKELKLLFGAMKAVNGKWPRHSAKLLNVLKQMPHRNGPDVFFSFPGRKGSAIVLPPLAKWPYENGFTFSTWFRLDPINSVNIEREKPYLYCFKTSKGVGYTAHFVGNCLVLTSMKVKGKGFQHCVKYEFQPRKWYMIAIVYIYNRWTKSEIKCLVNGQLASSTEMAWLVSTNDPFDKCYVGATPELDEERVFCGQMAAIYLFSEALTTQQICAMHRLGPGYKSQFRFDNECYLNLPDNHKRVSEAKAILSSLSPTPLAPTESTIHRTLVSGSTAAAAAAATSAAAADHHPSSFSSPTTVALASPGVALASAAPGSGGEPFDLVSVLYDGKLSSAIVFMYNPVATDGQLCLQSAPKGNLSYFVHTPHSLMLQDVKAVVTHSIHCTLNSIGGIQVLFPLFSQLDMPYEGTDVRKDPTLCLDSFANVESSQTVQQHMIQNRGFLVISFMLQRSSRDHLSLDVLGSFLSLTKYLVTCLSANSDLLLKQLLDHVLFNPSLWIYTPATVQARLYAYLATEFLSDTQIYSNVRRVSTVLQTVHTLKFYYWVVNPRAKSGITPKGLDGPRPAQKDILAIRAYILLFLKQLIMIGNGAKDDELQSILNYLMTMHEDENLHDVLQMLISLMSEHPSSMVPAFDVKHGVRTIFKLLAAESQLIRLQALKLLGFFLSRSTHKRKYDVMSPHNLYTLLAERLLLYEESVSLPTYNVLYEIMTEHISQQILYAKHPEPESHYRLENPMILKVVATLIRQSKQSEQLIEVKKLFLSDMTLLCNNNRENRRTVLQMSVWQEWLIAMAYIHPKNSEEQKLSDMVYSLFRMLLHHAIKYEYGGWRVWVDTLAIVHSKVSYEEFKLQFAQMYEHYEKHRTDNITDPALRQQRPISTISGWEHGANGSTGAGTGKEGELDMHGKCVKQMAVPPGTAVAPGCVCEPETGETMIDTSVMVKQQQAIAFQDAPAGSNGYVRGGEPDLLEEDETCLPDGPQDGMDGEEEEENDDADGVDEEEEEVEEEEEDEEQDEEEGDEQRAFGSGQQQVPGSEPTACSQTSATNTNTTTTEHVVKTIVDEIIDKSANMLVEQQQPQHGETKEAPAEPTSSPVIKDEEIELAVNEVVKMNQNSLKAVEGEGGDMDAGADSNQQCGDESTRGSVEPVGTSEPSTVSSSPSVVCVSDRSNDRATVPASPVLQGEPDARKVAAAVDSIVEELLDRCFPASEHEEGDGATALAAEMQEVVESIITDAVEKAEKGASTAVVGEQQQDGSGTDVQRHHHHHRHHPHHHHHHHHHHAHEGEEHQRISVVSDAMEGMAISEKQRAGGNSLIEEDEEEVEEEEEEEEEEEEEEEEDDGEVLVAETEEPLDASDERAGGAVSVDTASDGAATGTADSGKPNSNSTISTGSKRAVSVSTNTQQYLDAQSHPKQAAQPLQQQPTTASQTPPVQPMSYESGSSTAGGGGVKRSKSSSTRPMFSPGPTRPPFRIPEFKWSYIHQRLLSDVLFSLETDIQVWRSHSTKSVLDFVNSAENAIFVVNTVHLISQLADNLIIACGGLLPLLASATSPNSELDVLEPTQGMPLDVAVSFLQRLVNMADVLIFASSLNFSELEAEKNMSSGGILRQCLRLVCTCAVRNCLECKERTRGLYNGMSLKDIPGGVHLQALIRGAQSTSKTIIESLSGPMSPVKDPEKLLQDMDINRLRAVIYRDVEETKQAQFLSLAIVYFISVLMVSKYRDILEPPVELQIHRNSSPVIHRSTPTQGTQSMQEGDYEVIVVDENNSSVIADNDSHSSGPLSIKSVDSDGGSLNLNSTENDPQEVETSSEIMPDDNKPTNSNDESWTDVNLNDDGASELKPPRTDGGALGIGGVPGSNGSGVGTGAGSLGPMASALSGRMDGVTGASVNLGGGAGGTGGVKHESDIAVVRVPDGYGGQVVGPTGGSVRGRPEDLNLKAPFVSQLPLAIPSREASLTQKLEIALGPVCPLLREIMVDFAPFLSKTLVGSHGQELLMEGKGLTTFKNSHSVVELVMLLCSQEWQNSLQKHAGLAFIELINEGRLLSHAMKDHIVRVANEAEFILNRMRADDVLKHADFESQCAQTLLERREEERMCDHLITAARRRDNVIASRLLEKVRNIMGNRHGAWGDMNANYQKQIFWKLDAWEDDARRRKRMVQNPRGSSHPQATLKASLENGTTGGTVAAATSSTASTAATSADEAVGSGDTTTPTSARLAGGGGGVAREEIYSQIAVPRSQQPDLLDDSELLIEDRELDLDLTGPVNISTKAKLIAPGLVAPGTMSITSTEMYFEVDEENGEFQQIDQEVLKYCDHLHGKWYFSEIRAIFSRRYLLQNVALEIFLASRTSILFAFPDQHTVKKVIKALPRVGVGIKYGIPQTRRASMMSPRQLMRNSNMTQKWQRREISNFEYLMFLNTIAGRTYNDLNQYPVFPWVITNYESRELDLSQPSNYRDLSKPIGALNPSRREYFEERYETWDTPGIPPFHYGTHYSTAAFVLNWLIRIEPFTTMFLALQGGKFDHPDRLFSSVALSWKNCQRDTSDVKELIPEWYFLPEMFYNASDYRLGQRDDGSTVGDVELPPWAKTPEEFVRINRMALESEFVSCQLHQWIDLIFGYKQRGPEAMRATNVFYYLTYEGSVDLDTIGDPVTRDAIENQIRNFGQTPSLLLMEPHPPRSSAMHLSPMMFNTMPDDVCMSLKFHLNSPIIHISANTYPQLPLPSVVTVTAGHQFAVNRWNCQYTASIQSPSYAESTQNLNANLPLTMDPLLSQINGHNNSNQQNRRHLGDNFSQKLQIKSNCYVTTVDSRFLIACGFWDNSFRVFSTETAKIVQIIFGHFGVVTCLSRSECNITSDCYIASGSADCTILLWHWNARTQSIVGEGEIPTPRATLTGHETAVTSVVISAELGLVVSGSINGPVLVHTTFGDLLRSLEAPKDFISPENITLSREGFIVVNYDEGSVAAYTINGKLLRYESHNDNLQCMLLSRDGEYLMTAGNKGIVEVWRTFNLAPLYAFPACNSGIRSLALTHDQKYLLAGLATGSIIVFHIDFNRWHHEYQQRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8UZJ4</accession>
    <name>A0A6E8UZJ4_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Gustatory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Gustatory receptor which mediates acceptance or avoidance behavior, depending on its substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the insect chemoreceptor superfamily. Gustatory receptor (GR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8UZJ4"/>
    <dbReference type="EnsemblMetazoa" id="ACON001117-RB">
        <property type="protein sequence ID" value="ACON001117-PB"/>
        <property type="gene ID" value="ACON001117"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON001117-RC">
        <property type="protein sequence ID" value="ACON001117-PC"/>
        <property type="gene ID" value="ACON001117"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_003504"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001117"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_036433"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007635">
        <property type="term" value="P:chemosensory behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008049">
        <property type="term" value="P:male courtship behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050909">
        <property type="term" value="P:sensory perception of taste"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013604">
        <property type="entry name" value="7TM_chemorcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21143:SF104">
        <property type="entry name" value="GUSTATORY RECEPTOR 8A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21143">
        <property type="entry name" value="INVERTEBRATE GUSTATORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08395">
        <property type="entry name" value="7tm_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="37"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="74"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="123"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="163"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="283"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="323"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001117-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="368" mass="42544" checksum="5ABFD541214B851B" modified="2020-08-12" version="1">MEVLYWILRLFGYAPFRLQLDADTPDRDAFGTWRTKLYTISFGLVYTLAFIKFMSELKHTGFSASMIEGKGERMYFTFNFFTTMYGVVNGYAVRDKIEKMLYKLHAVDQKISQWKRNVDHRRFYRNVWAGVYLIAIVLLIYIYGTIITCQLHESSCNMQILFVFIYLLFVNSYALMMLQCTAFMEIIRTRYKLINGCFRMYFPTSPSAVGNPSEFAICSEKEQINVLKQIKILHDKLNDVVELVNYCFSVQGKRIGILVHKAINCSSSSAVINEVQCCKGANMMLPVCNVPCAWIILILYLSRQLNLFSQQLLHRSPVITCGLFVYDWTLWYTMIGATATYLIILIQFDVSFPNLVNVNATAVYRGST</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V163</accession>
    <name>A0A6E8V163_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Dynein heavy chain, cytoplasmic</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Dynein heavy chain, cytosolic</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">Ngousso</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Consists of at least two heavy chains and a number of intermediate and light chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynein heavy chain family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040224500.1">
        <property type="nucleotide sequence ID" value="XM_040368566.2"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON002015-RA">
        <property type="protein sequence ID" value="ACON002015-PA"/>
        <property type="gene ID" value="ACON002015"/>
    </dbReference>
    <dbReference type="GeneID" id="120950490"/>
    <dbReference type="CTD" id="38580"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010061"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002015"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040415"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904115">
        <property type="term" value="C:axon cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005858">
        <property type="term" value="C:axonemal dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008569">
        <property type="term" value="F:minus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048469">
        <property type="term" value="P:cell maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051642">
        <property type="term" value="P:centrosome localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051293">
        <property type="term" value="P:establishment of spindle localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051235">
        <property type="term" value="P:maintenance of location"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072384">
        <property type="term" value="P:organelle transport along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008090">
        <property type="term" value="P:retrograde axonal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.20:FF:000002">
        <property type="entry name" value="Cytoplasmic dynein 1 heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000122">
        <property type="entry name" value="Cytoplasmic dynein 1 heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.1220:FF:000002">
        <property type="entry name" value="cytoplasmic dynein 1 heavy chain 1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.2620:FF:000001">
        <property type="entry name" value="Cytoplasmic dynein heavy chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.130:FF:000002">
        <property type="entry name" value="Cytoplasmic dynein heavy chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.710:FF:000005">
        <property type="entry name" value="Cytoplasmic dynein heavy chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.100:FF:000002">
        <property type="entry name" value="Cytoplasmic dynein heavy chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1120:FF:000003">
        <property type="entry name" value="Cytoplasmic dynein heavy chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.30:FF:000001">
        <property type="entry name" value="Cytoplasmic dynein heavy chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.180.20:FF:000002">
        <property type="entry name" value="Cytoplasmic dynein heavy chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000071">
        <property type="entry name" value="Cytoplasmic dynein heavy chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000517">
        <property type="entry name" value="Cytoplasmic dynein heavy chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.720:FF:000003">
        <property type="entry name" value="Cytoplasmic dynein heavy chain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.280:FF:000004">
        <property type="entry name" value="Cytoplasmic dynein heavy chain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.490.20:FF:000004">
        <property type="entry name" value="Cytoplasmic dynein heavy chain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000373">
        <property type="entry name" value="Cytoplasmic dynein heavy chain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001956">
        <property type="entry name" value="Dynein cytoplasmic 1 heavy chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.1220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.100">
        <property type="entry name" value="Dynein heavy chain, N-terminal domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.180.20">
        <property type="entry name" value="Dynein heavy chain, N-terminal domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.720">
        <property type="entry name" value="Region D6 of dynein motor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035699">
        <property type="entry name" value="AAA_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035706">
        <property type="entry name" value="AAA_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041658">
        <property type="entry name" value="AAA_lid_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042219">
        <property type="entry name" value="AAA_lid_11_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054354">
        <property type="entry name" value="DYNC2H1-like_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042222">
        <property type="entry name" value="Dynein_2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043157">
        <property type="entry name" value="Dynein_AAA1S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041466">
        <property type="entry name" value="Dynein_AAA5_ext"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041228">
        <property type="entry name" value="Dynein_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043160">
        <property type="entry name" value="Dynein_C_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024743">
        <property type="entry name" value="Dynein_HC_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024317">
        <property type="entry name" value="Dynein_heavy_chain_D4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004273">
        <property type="entry name" value="Dynein_heavy_D6_P-loop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013602">
        <property type="entry name" value="Dynein_heavy_linker"/>
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    <dbReference type="InterPro" id="IPR013594">
        <property type="entry name" value="Dynein_heavy_tail"/>
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    <dbReference type="InterPro" id="IPR042228">
        <property type="entry name" value="Dynein_linker_3"/>
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    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46532:SF13">
        <property type="entry name" value="CYTOPLASMIC DYNEIN 1 HEAVY CHAIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46532">
        <property type="entry name" value="MALE FERTILITY FACTOR KL5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12774">
        <property type="entry name" value="AAA_6"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AAA_9"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AAA_lid_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08385">
        <property type="entry name" value="DHC_N1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08393">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22597">
        <property type="entry name" value="DYN_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17852">
        <property type="entry name" value="Dynein_AAA_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18199">
        <property type="entry name" value="Dynein_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03028">
        <property type="entry name" value="Dynein_heavy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12777">
        <property type="entry name" value="MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-0243">Dynein</keyword>
    <keyword evidence="6" id="KW-0493">Microtubule</keyword>
    <keyword evidence="12" id="KW-0505">Motor protein</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="17">
        <location>
            <begin position="1912"/>
            <end position="2063"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="17">
        <location>
            <begin position="2226"/>
            <end position="2377"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="17">
        <location>
            <begin position="2597"/>
            <end position="2747"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="17">
        <location>
            <begin position="2939"/>
            <end position="3105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="2399"/>
            <end position="2421"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="551"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="3218"/>
            <end position="3287"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="3413"/>
            <end position="3482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00033439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002015-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="4669" mass="533154" checksum="18F325EBD06D44C8" modified="2020-08-12" version="1">[encoded data, 4669 characters]</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V180</accession>
    <name>A0A6E8V180_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="16">V-SNARE coiled-coil homology domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the WD repeat L(2)GL family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040218728.1">
        <property type="nucleotide sequence ID" value="XM_040362794.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8V180"/>
    <dbReference type="EnsemblMetazoa" id="ACON000009-RB">
        <property type="protein sequence ID" value="ACON000009-PB"/>
        <property type="gene ID" value="ACON000009"/>
    </dbReference>
    <dbReference type="GeneID" id="120947447"/>
    <dbReference type="CTD" id="32217"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002418"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000009"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032600"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045159">
        <property type="term" value="F:myosin II binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15873">
        <property type="entry name" value="R-SNARE_STXBP5_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001702">
        <property type="entry name" value="AGAP000009-PB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000001">
        <property type="entry name" value="syntaxin-binding protein 5 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000664">
        <property type="entry name" value="Lethal2_giant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013577">
        <property type="entry name" value="LLGL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042855">
        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10241">
        <property type="entry name" value="LETHAL 2 GIANT LARVAE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10241:SF25">
        <property type="entry name" value="TOMOSYN, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08366">
        <property type="entry name" value="LLGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00957">
        <property type="entry name" value="Synaptobrevin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00962">
        <property type="entry name" value="LETHAL2GIANT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50892">
        <property type="entry name" value="V_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="8" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="V-SNARE coiled-coil homology" evidence="15">
        <location>
            <begin position="1127"/>
            <end position="1191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="658"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="894"/>
            <end position="917"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="669"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="14">
        <location>
            <begin position="692"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="747"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="763"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="773"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="786"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="894"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="906"/>
            <end position="916"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000009-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1192" mass="130615" checksum="6384173F8B2402A0" modified="2020-08-12" version="1">MKKFTFKGVLDGFRSSVNQQSAAGGGAAKICVEQEIQETLRSEHFQLKKTFRHGFPYSPTALAFDPVQRILAVGDKFGSMRLLGKFGVDAHVKHEGESACAVNLIEFLVNEGCLVTATADDTLHLWNFHTKIPKVVQSLKFQRERISRLHLPIGTKWLYIGTEKGNTHIVHVDSFTLSGYVINWNKAIDPIRKTHPGAVVHLSDNPLDANKLLIGFESGQLVLWDMRGKCAEFRWLSQEPLSSCSWHHEGKHFITSHTDGSLCTWPLKLSPKPLTHSFPHAKTNKEGKTESCNSIQKVEVKTNRIGDQFTIFSGGLPTEKCSPKSHCITVMVQGKSTTVLEMEHSVVDFVTMCESPWGSDLQEPYAVAVLLQNDLVLIDLLTPGYPTFESPYSMDLHESQITCCTYLADCPSDLVPAFYSVGRSAATNRRSGYSEREWPINGGEWQPTSCSYSEIILTGHQDGSIKFWDSSAGTLQVLYKLKTSKIFEKPRVRSMDSGEEDPLAISMMSLCAESRKLCVACNCGYVVLFKFRRAESTGDIVVLDIPFTMETFTEFEGSPECEFIPKSLPKTVDSTESDKKNCSLLKVKTGPQRKPPGFQAQLVCVPSWACGSVQITALTINSNYGLMAYGNEYGLTIVDIVKKTCVLNLCTQEMYGAQDPYSRTPRSPKRLENSQSKDDSLRSPSIDQINGLVGGGSGGGGGGSGSGGGGGSGAGGGIGSGTTSSSATTPNAVNSNMPLSMYERGMSISSAGSPSKRTGAGSGSTTITGLTDSVRKSRSQDKLDNSFSRSRSSSMSSLENNAMEAVTCLAFADSYVKKSDPSMALPTAWLGTSLGSILTISLSIPENDLRKANVVQTAPTGAMFRLKGSILSMSLLDCNGALIPYSFESWREDSKDSKEKRERTPTKTSSTKMSPTNPDGLCDRQFIAIASEKQAGVFALPSQNCVYRKKLVDFDFIVKAEIISMKDSVCLICYASNGHLMVFTLPSLKPLLEVDFLPLADLSFQTKCKQGVVDPMLSIWGQQLIVNEDSNVISRTFSFSNKGHGLYLATPTEIQKFTVCQEFSHQYNNMLGDLYVPHEMPEAPKESFFKGLFGGGMRNLDREELFGESSGKANRSVAKHIPGPNADIQALNARTTSVTSEIGKAHQLMLERGDKLSQLEERTERMANEAQQFSSSAHLLMNKYKDKKWYQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V186</accession>
    <name>A0A6E8V186_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Neurobeachin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON000017-RB">
        <property type="protein sequence ID" value="ACON000017-PB"/>
        <property type="gene ID" value="ACON000017"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009738"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000017"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032851"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06071">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01201">
        <property type="entry name" value="PH_BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1540.10:FF:000001">
        <property type="entry name" value="neurobeachin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000059">
        <property type="entry name" value="neurobeachin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000010">
        <property type="entry name" value="neurobeachin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1540.10">
        <property type="entry name" value="BEACH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000409">
        <property type="entry name" value="BEACH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036372">
        <property type="entry name" value="BEACH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050865">
        <property type="entry name" value="BEACH_Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046851">
        <property type="entry name" value="NBCH_WD40"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010508">
        <property type="entry name" value="NBEA-like_DUF1088"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031570">
        <property type="entry name" value="NBEA/BDCP_DUF4704"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046852">
        <property type="entry name" value="Neurobeachin_a-sol"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023362">
        <property type="entry name" value="PH-BEACH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13743">
        <property type="entry name" value="BEIGE/BEACH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13743:SF162">
        <property type="entry name" value="NEUROBEACHIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02138">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06469">
        <property type="entry name" value="DUF1088"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15787">
        <property type="entry name" value="DUF4704"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20426">
        <property type="entry name" value="NBCH_WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20425">
        <property type="entry name" value="Neurobeachin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14844">
        <property type="entry name" value="PH_BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01026">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81837">
        <property type="entry name" value="BEACH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50197">
        <property type="entry name" value="BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51783">
        <property type="entry name" value="PH_BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="BEACH-type PH" evidence="8">
        <location>
            <begin position="2483"/>
            <end position="2591"/>
        </location>
    </feature>
    <feature type="domain" description="BEACH" evidence="7">
        <location>
            <begin position="2610"/>
            <end position="2899"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="3195"/>
            <end position="3226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1096"/>
            <end position="1197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1211"/>
            <end position="1239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1259"/>
            <end position="1322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1384"/>
            <end position="1431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1447"/>
            <end position="1609"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1927"/>
            <end position="1952"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="2003"/>
            <end position="2071"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="2404"/>
            <end position="2446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="1126"/>
            <end position="1162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1180"/>
            <end position="1195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1289"/>
            <end position="1308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1392"/>
            <end position="1401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="1404"/>
            <end position="1424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="1457"/>
            <end position="1496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1508"/>
            <end position="1522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1523"/>
            <end position="1549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1554"/>
            <end position="1572"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1927"/>
            <end position="1940"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="2018"/>
            <end position="2037"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="2045"/>
            <end position="2057"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="2407"/>
            <end position="2424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000017-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="3283" mass="362663" checksum="50C6E58865B28BA8" modified="2020-08-12" version="1">MADLTRPPLCDIKRPEEVVKMSMTDNLKFAVLIGLIEVGQVSNREVVNTVLHLLVGGEFDMELNFVVQDAQNVKHMLELLDHCPANLQAEVWSVFIAILKKSVRNLQACTEIGLIEHVLARLQQAEPIVADLLIEMLGVLASYSITVKELKLLFGAMKAVNGKWPRHSAKLLNVLKQMPHRNGPDVFFSFPGRKGSAIVLPPLAKWPYENGFTFSTWFRLDPINSVNIEREKPYLYCFKTSKGVGYTAHFVGNCLVLTSMKVKGKGFQHCVKYEFQPRKWYMIAIVYIYNRWTKSEIKCLVNGQLASSTEMAWLVSTNDPFDKCYVGATPELDEERVFCGQMAAIYLFSEALTTQQICAMHRLGPGYKSQFRFDNECYLNLPDNHKRVSEAKAILSSLSPTPLAPTESTIHRTLVSGSTAAAAAAATSAAAADHHPSSFSSPTTVALASPGVALASAAPGSGGEPFDLVSVLYDGKLSSAIVFMYNPVATDGQLCLQSAPKGNLSYFVHTPHSLMLQDVKAVVTHSIHCTLNSIGGIQVLFPLFSQLDMPYEGTDVRKDPTLCLDSFANVESSQTVQQHMIQNRGFLVISFMLQRSSRDHLSLDVLGSFLSLTKYLVTCLSANSDLLLKQLLDHVLFNPSLWIYTPATVQARLYAYLATEFLSDTQIYSNVRRVSTVLQTVHTLKFYYWVVNPRAKSGITPKGLDGPRPAQKDILAIRAYILLFLKQLIMIGNGAKDDELQSILNYLMTMHEDENLHDVLQMLISLMSEHPSSMVPAFDVKHGVRTIFKLLAAESQLIRLQALKLLGFFLSRSTHKRKYDVMSPHNLYTLLAERLLLYEESVSLPTYNVLYEIMTEHISQQILYAKHPEPESHYRLENPMILKVVATLIRQSKQSEQLIEVKKLFLSDMTLLCNNNRENRRTVLQMSVWQEWLIAMAYIHPKNSEEQKLSDMVYSLFRMLLHHAIKYEYGGWRVWVDTLAIVHSKVSYEEFKLQFAQMYEHYEKHRTDNITDPALRQQRPISTISGWEHGANGSTGAGTGKEGELDMHGKCVKQMAVPPGTAVAPGCVCEPETGETMIDTSVMVKQQQAIAFQDAPAGSNGYVRGGEPDLLEEDETCLPDGPQDGMDGEEEEENDDADGVDEEEEEVEEEEEDEEQDEEEGDEQRAFGSGQQQVPGSEPTACSQTSATNTNTTTTEHVVKTIVDEIIDKSANMLVEQQQPQHGETKEAPAEPTSSPVIKDEEIELAVNEVVKMNQNSLKAVEGEGGDMDAGADSNQQCGDESTRGSVEPVGTSEPSTVSSSPSVVCVSDRSNDRATVPASPVLQGEPDARKVAAAVDSIVEELLDRCFPASEHEEGDGATALAAEMQEVVESIITDAVEKAEKGASTAVVGEQQQDGSGTDVQRHHHHHRHHPHHHHHHHHHHAHEGEEHQRISVVSDAMEGMAISEKQRAGGNSLIEEDEEEVEEEEEEEEEEEEEEEEDDGEVLVAETEEPLDASDERAGGAVSVDTASDGAATGTADSGKPNSNSTISTGSKRAVSVSTNTQQYLDAQSHPKQAAQPLQQQPTTASQTPPVQPMSYESGSSTAGGGGVKRSKSSSTRPMFSPGPTRPPFRIPEFKWSYIHQRLLSDVLFSLETDIQVWRSHSTKSVLDFVNSAENAIFVVNTVHLISQLADNLIIACGGLLPLLASATSPNSELDVLEPTQGMPLDVAVSFLQRLVNMADVLIFASSLNFSELEAEKNMSSGGILRQCLRLVCTCAVRNCLECKERTRGLYNGMSLKDIPGGVHLQALIRGAQSTSKTIIESLSGPMSPVKDPEKLLQDMDINRLRAVIYRDVEETKQAQFLSLAIVYFISVLMVSKYRDILEPPVELQIHRNSSPVIHRSTPTQGTQSMQEGDYEVIVVDENNSSVIADNDSHSSGPLSIKAVTSASNSRRTSTVKQYHPTDHPGLPAAVNTGEPHLHSCYQQPMLSSVGLTGTMATVTAPSATATIAVSDERSELAQRAVPQGQVKSVDSDGGSLNLNSTENDPQEVETSSEIMPDDNKPTNSNDESWTDVNLNDDGASELKPPRTDGGALGIGGVPGSNGSGVGTGAGSLGPMASALSGRMDGVTGASVNLGGGAGGTGGVKHESDIAVVRVPDGYGGQVVGPTGGSVRGRPEDLNLKAPFVSQLPLAIPSREASLTQKLEIALGPVCPLLREIMVDFAPFLSKTLVGSHGQELLMEGKGLTTFKNSHSVVELVMLLCSQEWQNSLQKHAGLAFIELINEGRLLSHAMKDHIVRVANEAEFILNRMRADDVLKHADFESQCAQTLLERREEERMCDHLITAARRRDNVIASRLLEKVRNIMGNRHGAWGDMNANYQKQIFWKLDAWEDDARRRKRMVQNPRGSSHPQATLKASLENGTTGGTVAAATSSTASTAATSADEAVGSGDTTTPTSARLAGGGGGVAREEIYSQIAVPRSQQPDLLDDSELLIEDRELDLDLTGPVNISTKAKLIAPGLVAPGTMSITSTEMYFEVDEENGEFQQIDQEVLKYCDHLHGKWYFSEIRAIFSRRYLLQNVALEIFLASRTSILFAFPDQHTVKKVIKALPRVGVGIKYGIPQTRRASMMSPRQLMRNSNMTQKWQRREISNFEYLMFLNTIAGRTYNDLNQYPVFPWVITNYESRELDLSQPSNYRDLSKPIGALNPSRREYFEERYETWDTPGIPPFHYGTHYSTAAFVLNWLIRIEPFTTMFLALQGGKFDHPDRLFSSVALSWKNCQRDTSDVKELIPEWYFLPEMFYNASDYRLGQRDDGSTVGDVELPPWAKTPEEFVRINRMALESEFVSCQLHQWIDLIFGYKQRGPEAMRATNVFYYLTYEGSVDLDTIGDPVTRDAIENQIRNFGQTPSLLLMEPHPPRSSAMHLSPMMFNTMPDDVCMSLKFHLNSPIIHISANTYPQLPLPSVVTVTAGHQFAVNRWNCQYTASIQSPSYAESTQNLNANLPLTMDPLLSQINGHNNSNQQNRRHLGDNFSQKLQIKSNCYVTTVDSRFLIACGFWDNSFRVFSTETAKIVQIIFGHFGVVTCLSRSECNITSDCYIASGSADCTILLWHWNARTQSIVGEGEIPTPRATLTGHETAVTSVVISAELGLVVSGSINGPVLVHTTFGDLLRSLEAPKDFISPENITLSREGFIVVNYDEGSVAAYTINGKLLRYESHNDNLQCMLLSRDGEYLMTAGNKGIVEVWRTFNLAPLYAFPACNSGIRSLALTHDQKYLLAGLATGSIIVFHIDFNRWHHEYQQRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V191</accession>
    <name>A0A6E8V191_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ubiquitin-like domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8V191"/>
    <dbReference type="EnsemblMetazoa" id="ACON002068-RB">
        <property type="protein sequence ID" value="ACON002068-PB"/>
        <property type="gene ID" value="ACON002068"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005934"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002068"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040189"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001669">
        <property type="term" value="C:acrosomal vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030317">
        <property type="term" value="P:flagellated sperm motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037695">
        <property type="entry name" value="IQUB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057887">
        <property type="entry name" value="IQUB_helical"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21074">
        <property type="entry name" value="IQ AND UBIQUITIN-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21074:SF0">
        <property type="entry name" value="IQ AND UBIQUITIN-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25805">
        <property type="entry name" value="IQUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IQ motif and ubiquitin-like" evidence="1">
        <location>
            <begin position="42"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF25805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002068-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="350" mass="40967" checksum="DACC93D707FC7E05" modified="2020-08-12" version="1">MKLIREKYSGAPKIAEMNILLDKEIQLLNGVERQRRAIRREQLEEVVERQLVKISEPKRWVGYNNRVIEMETPQTQRARFLLVYYRRLKMALPPSAGGDPQEHMALLDELADVIVQENHPATEELENLLQRERKLLLCHLRTEDLSILRKRQLTLLADIIRTDPEKARERPVTKLCRKCKKLRPANAFTVHTRQRSADVCGQCTSLLGSTVDIAVYRSILRAIRRDERKRGSLASYAFIIQENDIKHIVENIWHGHSVISNDTDRADLMLPRWNVAQDWSPWNCICLTEQETRAHLKLQRLDRFYQPSVLKEIQSKHALARSVFGQLREIDQEFTESGDWWQVGLDEKLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V2Y1</accession>
    <name>A0A6E8V2Y1_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Chloride channel protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the chloride channel (TC 2.A.49) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8V2Y1"/>
    <dbReference type="EnsemblMetazoa" id="ACON002891-RC">
        <property type="protein sequence ID" value="ACON002891-PC"/>
        <property type="gene ID" value="ACON002891"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001039"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002891"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032829"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005247">
        <property type="term" value="F:voltage-gated chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03683">
        <property type="entry name" value="ClC_1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3080.10:FF:000003">
        <property type="entry name" value="Chloride channel 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.580.10:FF:000026">
        <property type="entry name" value="Chloride channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.580.10:FF:000032">
        <property type="entry name" value="Chloride channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3080.10">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014743">
        <property type="entry name" value="Cl-channel_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050970">
        <property type="entry name" value="Cl_channel_volt-gated"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001807">
        <property type="entry name" value="ClC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45720">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45720:SF10">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00654">
        <property type="entry name" value="Voltage_CLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00762">
        <property type="entry name" value="CLCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81340">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 13" id="KW-0129">CBS domain</keyword>
    <keyword evidence="11 14" id="KW-0868">Chloride</keyword>
    <keyword evidence="10" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 14" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 14" id="KW-0813">Transport</keyword>
    <keyword evidence="5" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="230"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="273"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="378"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="414"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="460"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="503"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="568"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="599"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="638"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="671"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="17">
        <location>
            <begin position="721"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="68"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1053"/>
            <end position="1090"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1120"/>
            <end position="1152"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="781"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="90"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="1053"/>
            <end position="1065"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="1076"/>
            <end position="1090"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="1122"/>
            <end position="1142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU361221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002891-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1152" mass="128043" checksum="331B869863848BA0" modified="2020-08-12" version="1">MHIPIKDEYVRDFSFEPEFILRRKDSLDIQREKYHRKRLNSRRKRLKRNALLVLAKTAAIVPPPLGHQHRVVEKNDPGEMPVRPDGVENGSRNSETSACLPTTSSSSSRSSTTNMHTSSRSNAMLIDVPSPDDCPAGSGLDDLDPADKDEIRRSIVDIEIEEFYYMYGRYTKDLGEYAKDEARRLRLLERRRKKDDKARNKELLDVRDNKFFKAASWLWRNTFARLGEDWVFLALLGIIMALLSYVMDKGISMCTNSRVWLYRDLTNHPVLQYLAWVSLPVCLILFSAGFVHLVAPQSIGSGIPEMKTILRGVALKEYLTFKTLVAKVIGLTATLGSGMPLGKEGPFVHIASIVSQLLSKIITSFQSIYENESRNTEMLAAACAVGVGACFAAPIGGVLFSIEVTTTYFAVRNYWRGFFAAVCGATVFRLLAVWFQKADTVTAMFSTNFTSDFPFDPQELFVFALIGLVCGLGGALYVWVHRKYVLFMRSNKKMNKFLQKNRFLYPGIVALIVATLSFPLGFGKYIAGELSTHDQVHQLFSNFTWTKHELTVEQAAVVSNWRTPETNVFANLIIYLLYTFFMSIIASTIPVPSGMFIPVFKIGAAFGRIIGEAMHLWFPHGVRYGGMLAPIIPGGYSVVGAAAFSGAVTHTVSVGVIVFEMTGQITHIVPVMIAALISNAVAALLQPSMYDSIILIKKLPYLPDLLPSGSGMYNIYVEDFMVRDVRYIWKGISYQQLKEILKANKSLRSLPIVDSPDNRTLLGSVQRYELIKMIDKHIGREKRLEVAAKLMKEAEEKAREEQERQKREAAEAAKARRPSRFEVVPAPDILKLRERANNEMLPPQARRESNASLTSGPVFGATQPKKSILKKTNSFTLKGFSPLSPHSPVHTPYTTITGTESRIRSAFDIIFRKSATLQDVTPDPEIGSIGTPSIVGSEVAPLTPGISKKVQLPRERVIDMSPEDQKAWEMEEMAKPIDVGNLHIDPAPFQLVERTSILKVHSLFSMVGINHAYVTNVGRLVGVVALKELRTAIESVNNGTLTPETVKAKQEEEARALAQKGKESDNNNGISAEDPLLPKRPGDTFSDKKVNNTITSMDSALSNSDNCSDIELDNINIDHGDTAATTNGTITTTTTTTPSTKHTNNHSGSQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V2Y8</accession>
    <name>A0A6E8V2Y8_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Maleylacetoacetate isomerase</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>4-maleylacetoacetate = 4-fumarylacetoacetate</text>
            <dbReference type="Rhea" id="RHEA:14817"/>
            <dbReference type="ChEBI" id="CHEBI:17105"/>
            <dbReference type="ChEBI" id="CHEBI:18034"/>
            <dbReference type="EC" id="5.2.1.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>glutathione</name>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 5/6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the GST superfamily. Zeta family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8V2Y8"/>
    <dbReference type="EnsemblMetazoa" id="ACOM042483-RB">
        <property type="protein sequence ID" value="ACOM042483-PB"/>
        <property type="gene ID" value="ACOM042483"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON002898-RA">
        <property type="protein sequence ID" value="ACON002898-PA"/>
        <property type="gene ID" value="ACON002898"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_013990"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002898"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037102"/>
    <dbReference type="UniPathway" id="UPA00139">
        <property type="reaction ID" value="UER00340"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000075882">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016034">
        <property type="term" value="F:maleylacetoacetate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006559">
        <property type="term" value="P:L-phenylalanine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006572">
        <property type="term" value="P:L-tyrosine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03191">
        <property type="entry name" value="GST_C_Zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03042">
        <property type="entry name" value="GST_N_Zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000010">
        <property type="entry name" value="Maleylacetoacetate isomerase isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000041">
        <property type="entry name" value="Maleylacetoacetate isomerase isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005955">
        <property type="entry name" value="GST_Zeta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034330">
        <property type="entry name" value="GST_Zeta_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034333">
        <property type="entry name" value="GST_Zeta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01262">
        <property type="entry name" value="maiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42673">
        <property type="entry name" value="MALEYLACETOACETATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42673:SF4">
        <property type="entry name" value="MALEYLACETOACETATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13410">
        <property type="entry name" value="GST_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13409">
        <property type="entry name" value="GST_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-0585">Phenylalanine catabolism</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0828">Tyrosine catabolism</keyword>
    <feature type="domain" description="GST N-terminal" evidence="12">
        <location>
            <begin position="10"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="13">
        <location>
            <begin position="98"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002898-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="222" mass="25432" checksum="941D1E0F46E7C6C9" modified="2020-08-12" version="1">MANVDILPESQPILYSYWRSSCSWRVRIALNLKEIPYDIKPISLIKSGGEQHCNEYREVNPMEQVPALQIDGHTLIESVSIMYYLEETRPQRPLMPQDVLKRAKVREICEVIASGVQPLQNLIVLIHVGEEKKKEWAQHWITRGFRAIEKLLSTSAGKFCVGDEITLADCCLVPQVFNARRFHVDLRPYPIILRIDRELEGHPAFRAAHPSNQPDCPPEAAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V2Z8</accession>
    <name>A0A6E8V2Z8_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="6">U2 snRNP auxiliary factor large subunit</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8V2Z8"/>
    <dbReference type="EnsemblMetazoa" id="ACON002908-RC">
        <property type="protein sequence ID" value="ACON002908-PC"/>
        <property type="gene ID" value="ACON002908"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000665"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002908"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031915"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12232">
        <property type="entry name" value="RRM3_U2AF65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000097">
        <property type="entry name" value="U2 snRNP auxiliary factor large subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23139">
        <property type="entry name" value="RNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="46"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002908-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="144" mass="15955" checksum="DCDDC503D62A2A0D" modified="2020-08-12" version="1">MQLGDKKLIVQRASVGAKNSNAAVVAPVQIQVPGLSLVGSSGPPTEVLCLLNMVTPDELKDEEEYEDILEDIREECNKYGVVRSVEIPRPIEGVDVPGCGKVFVEFNSIVDCQKAQQALTGRKFSDRVVVTSYFDPDKYHRREF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V8S6</accession>
    <name>A0A6E8V8S6_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">thioredoxin-disulfide reductase (NADPH)</fullName>
            <ecNumber evidence="3">1.8.1.9</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Thioredoxin system is a major player in glutathione metabolism, due to the demonstrated absence of a glutathione reductase. Functionally interacts with the Sod/Cat reactive oxidation species (ROS) defense system and thereby has a role in preadult development and life span. Lack of a glutathione reductase suggests antioxidant defense in Drosophila, and probably in related insects, differs fundamentally from that in other organisms.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>[thioredoxin]-dithiol + NADP(+) = [thioredoxin]-disulfide + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20345"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.8.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="14">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.9" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8V8S6"/>
    <dbReference type="EnsemblMetazoa" id="ACON000565-RA">
        <property type="protein sequence ID" value="ACON000565-PA"/>
        <property type="gene ID" value="ACON000565"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_013156"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000565"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032148"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004362">
        <property type="term" value="F:glutathione-disulfide reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004791">
        <property type="term" value="F:thioredoxin-disulfide reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000190">
        <property type="entry name" value="Thioredoxin reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.390.30:FF:000004">
        <property type="entry name" value="Thioredoxin reductase 1, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046952">
        <property type="entry name" value="GSHR/TRXR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001100">
        <property type="entry name" value="Pyr_nuc-diS_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012999">
        <property type="entry name" value="Pyr_OxRdtase_I_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006338">
        <property type="entry name" value="Thioredoxin/glutathione_Rdtase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01438">
        <property type="entry name" value="TGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42737">
        <property type="entry name" value="GLUTATHIONE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42737:SF7">
        <property type="entry name" value="THIOREDOXIN-DISULFIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000350">
        <property type="entry name" value="Mercury_reductase_MerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="FAD/NAD-linked reductases, dimerisation (C-terminal) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00076">
        <property type="entry name" value="PYRIDINE_REDOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 14 16" id="KW-0274">FAD</keyword>
    <keyword evidence="4 16" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="14" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 16" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10 16" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="18">
        <location>
            <begin position="11"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="Pyridine nucleotide-disulphide oxidoreductase dimerisation" evidence="17">
        <location>
            <begin position="373"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13">
        <location>
            <position position="475"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="197"/>
            <end position="204"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="292"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="337"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="15">
        <location>
            <begin position="57"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00054062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU003691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000565-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="502" mass="54468" checksum="4CDF815DFA53B74A" modified="2020-08-12" version="1">MAPLNQENYEYDLVVIGGGSGGLACAKQAVQLGAKVAVLDFVKPSPRGTKWGLGGTCVNVGCIPKKLMHQASLLGEAIHDSQPYGWQLPDPAAIRHDWATLTESVQNHIKSVNWVTRVDLRDQKVEYVNGLGYFKDDHTVVAVMKNQTERELRAKHVVIAVGGRPRYPDIPGAAEYGITSDDIFSLPQAPGRTLLVGAGYIGLECAGFLKGLGYDVSVMVRSILLRGFDQQMATMVGDSMVEKGIRFHHRSRPLAVEKQPDGRLLVRYETVDEAGTATNGEDVFDTVLFAIGRQAETSTLKLANAGVVTAEGGKSDKLEVDETDHRTNVPHIYAVGDVLYRKPELTPVAIHAGRIIARRLFGGSEERMDYADVATTVFTPLEYGCVGLSEEAAEAAHGKDGIEVYHAYYKPTEFFVPQRSVRYCYLKAVALREGNQRVLGLHFLGPAAGEVIQGFAAALKCGLTMQVLRNTVGIHPTVAEEFTRLAITKSSGLDPTPATCCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V9F2</accession>
    <name>A0A6E8V9F2_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Tropomodulin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8V9F2"/>
    <dbReference type="EnsemblMetazoa" id="ACON000969-RD">
        <property type="protein sequence ID" value="ACON000969-PD"/>
        <property type="gene ID" value="ACON000969"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006835"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON000969"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_036786"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030016">
        <property type="term" value="C:myofibril"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005523">
        <property type="term" value="F:tropomyosin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030239">
        <property type="term" value="P:myofibril assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051694">
        <property type="term" value="P:pointed-end actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000099">
        <property type="entry name" value="Tropomodulin, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004934">
        <property type="entry name" value="TMOD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10901">
        <property type="entry name" value="TROPOMODULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10901:SF6">
        <property type="entry name" value="TROPOMODULIN, ISOFORM N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03250">
        <property type="entry name" value="Tropomodulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="49"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000969-PD"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="390" mass="43735" checksum="2443F7A7B12F48CA" modified="2020-08-12" version="1">MTSSSTTTKTITTPAKLYGKDVGAYDDIDVDELLAQLSPEEITMLAKEVDPDDSFLPPSQRTNYECDKAPTGPLDRKKLIDHINKQALETPDRPELEPFVPGVVRGKKWIPPPQAAKLQAADEQIAIDLGDEYEQALSDATQEEIIDLAAILGFHSMMNQDQYHASLLNKGQPVGLGWDGITKSAIPKIFPAEAPNTTDPEETIQRIKSDDSKLVEVNLNNIKTISDEQFDKLFEALKSNTHLEVLSLTNVGLTDRTALMLAAALEQNHALRVLNVETNFISPTVIVTLVKSLLTQMTIEEFRASNQRSQVFGNKIEMEITELVEKNTTLLRLGLHLEFNDARHRVAAHLQRNIDRNDPEHCHPIPYYRFLHVPIPTEPVPTKRTWEELP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V9F3</accession>
    <name>A0A6E8V9F3_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Troponin I</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the troponin I family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040220292.1">
        <property type="nucleotide sequence ID" value="XM_040364358.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8V9F3"/>
    <dbReference type="EnsemblMetazoa" id="ACON001053-RE">
        <property type="protein sequence ID" value="ACON001053-PE"/>
        <property type="gene ID" value="ACON001053"/>
    </dbReference>
    <dbReference type="GeneID" id="120948222"/>
    <dbReference type="CTD" id="32794"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007438"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001053"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039909"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005861">
        <property type="term" value="C:troponin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006936">
        <property type="term" value="P:muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.350:FF:000004">
        <property type="entry name" value="WupA, isoform I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001978">
        <property type="entry name" value="Troponin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050875">
        <property type="entry name" value="Troponin_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038077">
        <property type="entry name" value="Troponin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13738">
        <property type="entry name" value="TROPONIN I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13738:SF1">
        <property type="entry name" value="TROPONIN I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00992">
        <property type="entry name" value="Troponin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90250">
        <property type="entry name" value="Troponin coil-coiled subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="2" id="KW-0488">Methylation</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="180"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="180"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00074692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001053-PE"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="210" mass="24707" checksum="C774F0F70A27FD5F" modified="2020-08-12" version="1">MADDEAKKAKQAEIERKRAEVRKRMEEASKAKKAKKGFMTPERKKKLRLLLRKKAAEELKKEQERKAAERRRIIEERCGKPKNVEDANEAELQTICKEYWNRVFGLEGDKFDLERTEKLKQYEISDLNAQVNDLRGKFVKPTLKKVSKYENKFAKLQKKAAEFNFRNQLKVVKKKEFTLEEEDKEKKPDWSKKGDSKVKEEAAEAVEAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V9H5</accession>
    <name>A0A6E8V9H5_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Glycoside hydrolase family 31 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the glycosyl hydrolase 31 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8V9H5"/>
    <dbReference type="EnsemblMetazoa" id="ACON001111-RB">
        <property type="protein sequence ID" value="ACON001111-PB"/>
        <property type="gene ID" value="ACON001111"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002591"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001111"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037562"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06592">
        <property type="entry name" value="GH31_NET37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050985">
        <property type="entry name" value="Alpha-glycosidase_related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048395">
        <property type="entry name" value="Glyco_hydro_31_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000322">
        <property type="entry name" value="Glyco_hydro_31_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43053">
        <property type="entry name" value="GLYCOSIDASE FAMILY 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43053:SF6">
        <property type="entry name" value="SITS-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01055">
        <property type="entry name" value="Glyco_hydro_31_2nd"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21365">
        <property type="entry name" value="Glyco_hydro_31_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="106"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 31 TIM barrel" evidence="5">
        <location>
            <begin position="409"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 31 TIM barrel" evidence="5">
        <location>
            <begin position="580"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 31 C-terminal" evidence="6">
        <location>
            <begin position="696"/>
            <end position="778"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF21365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001111-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="785" mass="89828" checksum="82995EA76086464A" modified="2020-08-12" version="1">MVNQCSVQRECEMEEFVVEPKYATFSHFGQTGNLFRTKRRSSIAPLPALRLNDKEMMSNDFDTHSVLSNQASITSVNSLASLLKEKMQNVPAIIRKKKRETKDYKLRVFVGMLFLIIVFLVGYAYVMYNQKLLAKSYFESIKFHKKTRNFRLLDGKGGELLTGVLGTSLNIEQPYNCLPKDLKDDGSVCYEWNSKARLYMNLAKSPGPDIKCYSFQWESLLADLYPTDCFDVSEERGFWYGGGLTKGIEYILGKATIPAGPFVTGDINMHQWGNALKRYFLNSRGVAIQVDEKTPLYVSVNTDSGKRLCLQGKNDKFAFVNQQTRFPELKYTVCSGPDMKTLHNGLMQKSLWDGLTERDNDVIKSLLREPVWQIPASKQEDLTEVAIYNYTESIIALGYLRLGHVLVNEFWQRTIGDFRLDSERFPTLDETVNILHRRGFRVSFMIQPFISTESSNFREAVSKKLLIFERESERSIPALTRYKSTTSAGVLDVTNNASIPWLAEQLKKISETVEIDSYFVDFGTAFNIPRYYQCSQTLVNPDEYKNYFMERFEGTLSIFGVSSAISVPRPPAFLSLPPVNSSWSGLQSIIPTMLSYGIIGFPFLMPGPVGGDFVLPTQQLKKMYSYYSFELPPLPDKELYIRWLQLATFLPVLRFTHLPSEYRDETVTVIAKELADIRQRVLPLLERFSSIAMDEGLPIIRPLWMLDSTDVNCFSIDDEFSIGDGMIVAPVLKKGETVREVYLPQGVWKDGIDESLRKGSRWIHNYHVPQNKVAYFIKMPDNTRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V9H9</accession>
    <name>A0A6E8V9H9_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Gustatory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Gustatory receptor which mediates acceptance or avoidance behavior, depending on its substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the insect chemoreceptor superfamily. Gustatory receptor (GR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8V9H9"/>
    <dbReference type="EnsemblMetazoa" id="ACON001117-RD">
        <property type="protein sequence ID" value="ACON001117-PD"/>
        <property type="gene ID" value="ACON001117"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_003504"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001117"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_036433"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007635">
        <property type="term" value="P:chemosensory behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008049">
        <property type="term" value="P:male courtship behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050909">
        <property type="term" value="P:sensory perception of taste"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013604">
        <property type="entry name" value="7TM_chemorcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21143:SF104">
        <property type="entry name" value="GUSTATORY RECEPTOR 8A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21143">
        <property type="entry name" value="INVERTEBRATE GUSTATORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08395">
        <property type="entry name" value="7tm_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="43"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="85"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="177"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="295"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="335"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001117-PD"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="380" mass="43010" checksum="C6DD84E2B2D8273C" modified="2020-08-12" version="1">TMDSCLLVQNVHDTFRPFYYLLKVTGLAPFRLNSQPSNDRKELYYAFGYSASFICVYSYAIYGYLFTTNTGHLYISKIVAVMENGYMFCEFMVTNVAIILGLVLRNRVLEIFVQLAEIDEQLNVLRLPIKHNRQHRLLTVLCSIVLGSFLFLLLVTISTVTSRLVTSFDTPALDVAALAISSFCFLLQIVQFTVMVLLVLSRYQTINELFRMYFPTSPSAVGNPSEFAICSEKEQINVLKQIKILHDKLNDVVELVNYCFSVQGKRIGILVHKAINCSSSSAVINEVQCCKGANMMLPVCNVPCAWIILILYLSRQLNLFSQQLLHRSPVITCGLFVYDWTLWYTMIGATATYLIILIQFDVSFPNLVNVNATAVYRGST</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V9I7</accession>
    <name>A0A6E8V9I7_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8V9I7"/>
    <dbReference type="EnsemblMetazoa" id="ACON001141-RA">
        <property type="protein sequence ID" value="ACON001141-PA"/>
        <property type="gene ID" value="ACON001141"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009824"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001141"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030361"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="205"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="98"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="164"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="226"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001141-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="627" mass="69595" checksum="725A9A765BB9193E" modified="2020-08-12" version="1">MSKTKMAPVAGVTLHLECWRDELSPAQTAAYEKAEKEHHEVRNKLKEIVQETGGKNIFAGQVFTAYQVLGPVPGLEQISKPVTALSRKKMPSMPPALAPLGSSSSPEPSASFRRRSRKADSPAGENPEPVVDIASDDPDEDYIPLAKRMKQKAKEKTTPVPAKKTREEKVKTPDPKIKSPKTPGLTPKVAAISVRPTEFNSIGGLQVGAEKDKKRSAAEPSAVVDLTKDDSGKPTADSREISFSKIQGKTFPSLVVLARPSLRVVDKAVTDRPQLDAKVKNVLVHPAPKFTEWLIQQGLIKSEQTCQIHNDKPLKLGMYSDTSKFPYSGGYFWISDCCPTRFTSVFHGSLFEGSPHPPSVILKLIYHWACQTNVSNVVNWVKVDNMYLKGLYTLLRSVCTLALCRHMKLLGGPKKSIEIGVISLGTTTQDGQQRQVKVEVLGVLDPEAKILRLRAVEPLLDAEKSYKKRFSKILEPLSDWVHKDSVILTDLTVDKGTLNSMGYKTVIQVPGSEQTLKNSNANIMDYLRRIVPRMFQNTLSLLSRQIIQQFLNELVWRELYGNSPGTTFDNIVKHLSEEAKIETKESLTTRLHKAAADPFKQWEIVSDMPALGKVPGKRGRKPKDQSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V9K2</accession>
    <name>A0A6E8V9K2_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Troponin I</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the troponin I family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_049463048.1">
        <property type="nucleotide sequence ID" value="XM_049607091.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8V9K2"/>
    <dbReference type="EnsemblMetazoa" id="ACON001053-RB">
        <property type="protein sequence ID" value="ACON001053-PB"/>
        <property type="gene ID" value="ACON001053"/>
    </dbReference>
    <dbReference type="GeneID" id="120948222"/>
    <dbReference type="CTD" id="32794"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007438"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001053"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039909"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005861">
        <property type="term" value="C:troponin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006936">
        <property type="term" value="P:muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.350:FF:000004">
        <property type="entry name" value="WupA, isoform I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001978">
        <property type="entry name" value="Troponin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050875">
        <property type="entry name" value="Troponin_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038077">
        <property type="entry name" value="Troponin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13738">
        <property type="entry name" value="TROPONIN I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13738:SF1">
        <property type="entry name" value="TROPONIN I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00992">
        <property type="entry name" value="Troponin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90250">
        <property type="entry name" value="Troponin coil-coiled subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="2" id="KW-0488">Methylation</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00074692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001053-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="197" mass="23423" checksum="54CDEFFD1DCC397C" modified="2020-08-12" version="1">MADDEAKKAKQAEIERKRAEVRKRMEEASKAKKAKKGFMTPERKKKLRLLLRKKAAEELKKEQERKAAERRRIIEERCGKPKNVEDANEAELQTICKEYWNRVFGLEGDKFDLERTEKLKQYEISDLNAQVNDLRGKFVKPTLKKVSKYENKFAKLQKKAAEFNFRNQLKVVKKKEFTLEEEDKEAKKSEKADWQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V9L1</accession>
    <name>A0A6E8V9L1_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glycogen debranching enzyme</fullName>
            <ecNumber evidence="5">2.4.1.25</ecNumber>
            <ecNumber evidence="6">3.2.1.33</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Glycogen debrancher</fullName>
        </alternativeName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="22">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Multifunctional enzyme acting as 1,4-alpha-D-glucan:1,4-alpha-D-glucan 4-alpha-D-glycosyltransferase and amylo-1,6-glucosidase in glycogen degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>Hydrolysis of (1-&gt;6)-alpha-D-glucosidic branch linkages in glycogen phosphorylase limit dextrin.</text>
            <dbReference type="EC" id="3.2.1.33"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Transfers a segment of a (1-&gt;4)-alpha-D-glucan to a new position in an acceptor, which may be glucose or a (1-&gt;4)-alpha-D-glucan.</text>
            <dbReference type="EC" id="2.4.1.25"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the glycogen debranching enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.25" evidence="5"/>
    <dbReference type="EC" id="3.2.1.33" evidence="6"/>
    <dbReference type="RefSeq" id="XP_040228686.2">
        <property type="nucleotide sequence ID" value="XM_040372752.2"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON001200-RB">
        <property type="protein sequence ID" value="ACON001200-PB"/>
        <property type="gene ID" value="ACON001200"/>
    </dbReference>
    <dbReference type="GeneID" id="120953075"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002947"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001200"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037443"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004134">
        <property type="term" value="F:4-alpha-glucanotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004135">
        <property type="term" value="F:amylo-alpha-1,6-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005980">
        <property type="term" value="P:glycogen catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11327">
        <property type="entry name" value="AmyAc_Glg_debranch_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000382">
        <property type="entry name" value="AGAP001200-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000108">
        <property type="entry name" value="glycogen debranching enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000056">
        <property type="entry name" value="Uncharacterized protein, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010401">
        <property type="entry name" value="AGL/Gdb1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032788">
        <property type="entry name" value="AGL_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029436">
        <property type="entry name" value="AGL_euk_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032792">
        <property type="entry name" value="AGL_glucanoTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032790">
        <property type="entry name" value="GDE_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006421">
        <property type="entry name" value="Glycogen_debranch_met"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01531">
        <property type="entry name" value="glyc_debranch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10569">
        <property type="entry name" value="GLYCOGEN DEBRANCHING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10569:SF2">
        <property type="entry name" value="GLYCOGEN DEBRANCHING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06202">
        <property type="entry name" value="GDE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14701">
        <property type="entry name" value="hDGE_amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14702">
        <property type="entry name" value="hGDE_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14699">
        <property type="entry name" value="hGDE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0320">Glycogen biosynthesis</keyword>
    <keyword evidence="15" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="9" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Eukaryotic glycogen debranching enzyme N-terminal" evidence="19">
        <location>
            <begin position="32"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="Glycogen debranching enzyme glucanotransferase" evidence="20">
        <location>
            <begin position="140"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="domain" description="Glycogen debranching enzyme central" evidence="21">
        <location>
            <begin position="716"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="domain" description="Glycogen debranching enzyme C-terminal" evidence="18">
        <location>
            <begin position="1078"/>
            <end position="1520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00020723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00025780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00031477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF06202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF14699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF14701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF14702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001200-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1532" mass="173143" checksum="E0F6C8AE89895E5B" modified="2020-08-12" version="1">MADGVISIKLAIHDSNQGDGALYRLKKNSLVHVHPGASLLGRRVALYCNYPADDKEFCRKTYRRLEWCSAAGSPLETSASGSDYTSIKDVDYYCNVHFTKSGAYHFYALYEECPEAGAQAGLYVQIEPQLSVGSGEAATTLPLDAIRCQTVISKLLGPLDTWEAKLRVGKESGYNMIHFTPVQELGGSRSAYSLRNQLKVNPDFQSKGGKEVSYDDLAVLMRKMREEWNVASICDIVLNHTANESIWIREYPECSYNCFTCPHLRPAFLLDAMLARVGEDVKDGMLAHVGVPEVVETEDHLQAIRWQIHSNYLPQVKLYELYQCDVDKYVSRFNDECSKRSPSRAQDIPEGDIKLRMDPEHRRLGAEIDFERAMSIYNVFRQDCFDEDSRKRKCTEVFRARLQFLNEEVRREIQEHLDYAIDNCLAGTRYERVQGDGPQVKGISVKYPLFMKYFTHYGTQGKSLKDIESMMYGGAGKLFMAHNGWVMNGDPLKDFARAQPGTGNVYLRRELIAWGDSVKLRYGDKPEDSPYLWKHMQEYVDTTARIFDGVRLDNCHSTPLHVAEYLLDSARKVNPELYVAAELFTNSDHTDNIFVNRLGITSLIREALSAWDSHEQGRLVYRYGGAPVGAFFPCPKRILAPGIAHALFMDLTHDNPSPVEKRSIFDLIPSAGLVSMACCATGSNRGYDEFVPHHVHVVDEERQYQEWGKHVDKNTGLIAVKEAFNVLHGGLASRGFDQLFVDQMHPDIVAVTRHSLSNHETVILVAHTAFSYPNPWAGPTGVRPLEFEGEFKEIILEVQIYKKTGQTFDRPTDFAKNVDYINGVNEYVVDLKRNLKLEESDIFAKEAVVKGNVTQLHFSNLKPGSVVAVRVALKESVRVHFDTLQSLVREFHQDRGSRYAELQHILSKLDLVDFNKLLYCCAEEERDNGGGPYNVEGYGDLVYCGLQGVMSILSDIGPNNDLGHPLANNLRNGNWLIDYCSQRLMKYEKLVPMAKWLENNLKSLKEIPRYMIPSYFDVIITNVHRLAMAAACNLMSDFVRHGSNFARRLALGSVQCVADCPSASLPKLSPNVSDPKPPSHCATMAAGLPHFATGYMRCWGRDTFIAIRGLTLLTGRFDETRYMILGFGGCLRHGLIPNLLDGGQNARFNCRDAVWWWLYTIKQYVEEAPNGKSILKDKVSRLYPTDDSEAKAPGECDQMLYEVIQEALTVHFQGLSYRERNAGTRIDAHMKEKGFNNRIGINPDTGFVFGGNNANCGTWMDKMGSSDKAGNRGIPSTPRDGSAVELVGLQMASLRFMQAMAEQKVIPVSSVERTSYNGTKTVWTYKEWANKIAANFEKEFYVDESCESNYANKRGIYKDTVGSEIAWTDYQLRCNFPIALLAAPELADPKHAWRALENAKKYLLGPLGMKTLDYEDWGYRGNYDNSIDSDDKTVAHGANYHNGPEWVWPIGFYLRARLIFAKANGVLKETVAETWKILQTHLNELQRCHWRGLAELTNENGAYCKDSCRTQAWSMGCVLEVLYDLEKYEKEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VAD1</accession>
    <name>A0A6E8VAD1_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="15">G_PROTEIN_RECEP_F1_2 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VAD1"/>
    <dbReference type="EnsemblMetazoa" id="ACON001773-RC">
        <property type="protein sequence ID" value="ACON001773-PC"/>
        <property type="gene ID" value="ACON001773"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004751"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001773"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039090"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000405">
        <property type="entry name" value="Galanin_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45695:SF23">
        <property type="entry name" value="GALANIN-LIKE G-PROTEIN COUPLED RECEPTOR NPR-9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45695">
        <property type="entry name" value="LEUCOKININ RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00663">
        <property type="entry name" value="GALANINR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="38"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="119"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="158"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="58"/>
            <end position="183"/>
        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VAV8</accession>
    <name>A0A6E8VAV8_ANOCL</name>
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        <recommendedName>
            <fullName evidence="3 8">Mediator of RNA polymerase II transcription subunit 31</fullName>
        </recommendedName>
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        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
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            <taxon>Insecta</taxon>
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            <taxon>Anophelinae</taxon>
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        </lineage>
    </organism>
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        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
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                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <text evidence="8">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
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        <text evidence="8">Component of the Mediator complex.</text>
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        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Mediator complex subunit 31 family.</text>
    </comment>
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        <property type="gene ID" value="ACON002258"/>
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    </dbReference>
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    </evidence>
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</entry>
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        <name type="scientific">Anopheles coluzzii</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
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                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
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                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">Ngousso</strain>
        </source>
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    <dbReference type="AlphaFoldDB" id="A0A6E8VAW8"/>
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        <property type="gene ID" value="ACON002272"/>
    </dbReference>
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    <dbReference type="VEuPathDB" id="VectorBase:ACON002272"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033660"/>
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        <property type="component" value="Unplaced"/>
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    </dbReference>
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        <property type="match status" value="3"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        </source>
    </evidence>
    <sequence length="1146" mass="124362" checksum="D0D1886B97B8D106" modified="2020-08-12" version="1">MTSDGNTSFLRAARAGNLEKVLEHLKNNIDINTCNANGLNALHLASKDGHVAVVTELLARGATVDAATKKGNTALHIASLAGQEDVVKLLIKHNASVNVQSQNGFTPLYMAAQENHDSVVRLLLSNGANQSLATEDGFTPLAVAMQQGHDKVVAVLLESDTRGKVRLPALHIAAKKDDVKAATLLLENDHNPDVTSKSGFTPLHIASHYGNEAMANLLIQKGADVNYAAKHNISPLHVAAKWGKTNMVALLLEKGASIESKTRDGLTPLHCAARSGHEQVVDMLLERGAPISSKTKNGLAPLHMAAQGEHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRIKVVELLLKHGASISATTESGLTPLHVASFMGCMNIVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQVDAVTKDMYTALHIAAKEGQDEVAAVLLNNGAQIDATTKKGFTPLHLTAKYGHMKVAELLLEKSAPVDAQGKNGVTPLHVASHYDHQNVAMLLLEKGASPHATAKNGHTPLHIAARKNQIDIANTLLKYEAQANAESKAGFTPLHLSAQEGHTEMSGLLLESKANPDHQARNGLTPMHLCAQEDRVSVAQVLVKHGANLQAATKAGYTPLHVASHFGQANMVRYLIEQQVDVNASTGIGYTPLHQASQQGHCHIVNILLESNADPNAITNNGQTSLKIAQKLGYISVLDSLKSVTDAKATPDQPPSEEKYRVVAPEAMHETFMSDSEEEGGEDTVLSDQPYRYLTVDEMKSLGDDSLPIDVTRDERMDSNKMVQSTDSHQFPPTALEDSISPQHASMVQSGISATDFTDNINIERHSHVGKLHWKNFLVSFLVDARGGAMRGCRHSGVRIIVPARSAAQPTRITCRYVKPQRTMHPPPLMEGEALASRVLELGPVGAKFLGPVIMEVPHFASLRGKEREIVILRSDNGETWREHNIDMSDEIIHDVLNECFEPEELAQLDELGGGRICRFVTYDFPQYFAVISRIRQEVHAIGPEGGMVSSTVVPQVQAVFPQGALTKKIKVGLQVNLFKPRKGVAPEKLRKISVNHVPKKKRFSLIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VAZ3</accession>
    <name>A0A6E8VAZ3_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phenoloxidase-activating factor 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Prophenoloxidase-activating factor II</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase S1 family. CLIP subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040227850.2">
        <property type="nucleotide sequence ID" value="XM_040371916.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VAZ3"/>
    <dbReference type="EnsemblMetazoa" id="ACOM023586-RB">
        <property type="protein sequence ID" value="ACOM023586-PB.2"/>
        <property type="gene ID" value="ACOM023586"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON001964-RB">
        <property type="protein sequence ID" value="ACON001964-PB"/>
        <property type="gene ID" value="ACON001964"/>
    </dbReference>
    <dbReference type="GeneID" id="120952531"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007906"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001964"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038489"/>
    <dbReference type="Proteomes" id="UP000075882">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000038">
        <property type="entry name" value="Serine protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24258:SF129">
        <property type="entry name" value="LP15124P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24258">
        <property type="entry name" value="SERINE PROTEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044631290" description="Phenoloxidase-activating factor 2" evidence="7">
        <location>
            <begin position="25"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="8">
        <location>
            <begin position="100"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00068096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00076468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001964-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="358" mass="40376" checksum="06C89DC2B5BC958A" modified="2020-08-12" version="1">MPVRSGFLLLLLWLHQRLPASSQATEPATRRCSGGLCILPELCRPVSVNVYDEVHLDFFSSEECRFPHLKCCPFERIVRAPDTEAPDEAEIVCAARNNNGIGHVEQKDKTRAKYGEFPWMAFVYTAQADYELYLCGGTLVHSKVVITIAHCIENRTASELRVRLGEWDLEHMVEIYPPQDRAVIAAVTHPQFYSELLLNDIAILFLDEHVDFTEVVGIACLPPQNANFDHKRCLFTGWGEDERGRNSSVLKRTKLPIVPNGQCQRVLRRHLLNRSFRLHQGFLCAGGESGKDACRGDGGSPLVCPIPQSENQYYVVGLVAFGYECGTQGVPGVYVNVPHYRDWIDGEIEKMYHVDIFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VCH1</accession>
    <name>A0A6E8VCH1_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Fatty acid desaturase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">Ngousso</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">The histidine box domains are involved in binding the catalytic metal ions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the fatty acid desaturase type 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VCH1"/>
    <dbReference type="EnsemblMetazoa" id="ACON003049-RB">
        <property type="protein sequence ID" value="ACON003049-PB"/>
        <property type="gene ID" value="ACON003049"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000039"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003049"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031541"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004768">
        <property type="term" value="F:stearoyl-CoA 9-desaturase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006636">
        <property type="term" value="P:unsaturated fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03505">
        <property type="entry name" value="Delta9-FADS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015876">
        <property type="entry name" value="Acyl-CoA_DS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11351">
        <property type="entry name" value="ACYL-COA DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11351:SF21">
        <property type="entry name" value="GH07782P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00075">
        <property type="entry name" value="FACDDSATRASE"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="3 11" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="103"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="128"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003049-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="340" mass="38698" checksum="68231944497D6592" modified="2020-08-12" version="1">MTVGITSTLQFIAGNSAQYMKPGPNGQSQQAAAAAAAAAASSTVPDTNNNEMEDDRTVSDKDIDSYLKHYNRWQQSAWGRTLSDLVKRYLGYEFEDEVKWNNVFMIGIFHVIAVWSFLHYVWQATPITYLWGFFVGGCAGFGVTGGVHRLWCHRSYKAKLPLRIILMCCYSIAGQNTIYDWVRDHRIHHKYSETNGDPHNANRGFLYAHVGWLMLRKHPECIKKGRLIDMSDIVSDPVVQFHHKRINPAENRAVSVVAMGEGWHNYHHVFPWDYKAAELGNYSVNVTTFWLDVFAKIGWAYDLKEPSKDLVRRTIEKYGDGTHITARIGHMEEVPAPADS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VEL5</accession>
    <name>A0A6E8VEL5_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Synaptosomal-associated protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Synapse</location>
            <location evidence="6">Synaptosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the SNAP-25 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040218447.1">
        <property type="nucleotide sequence ID" value="XM_040362513.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VEL5"/>
    <dbReference type="SMR" id="A0A6E8VEL5"/>
    <dbReference type="EnsemblMetazoa" id="ACON001394-RB">
        <property type="protein sequence ID" value="ACON001394-PB"/>
        <property type="gene ID" value="ACON001394"/>
    </dbReference>
    <dbReference type="GeneID" id="120947307"/>
    <dbReference type="CTD" id="6616"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001443"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001394"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033016"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031629">
        <property type="term" value="P:synaptic vesicle fusion to presynaptic active zone membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016082">
        <property type="term" value="P:synaptic vesicle priming"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15885">
        <property type="entry name" value="SNARE_SNAP25C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15889">
        <property type="entry name" value="SNARE_SNAP25N_23N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000007">
        <property type="entry name" value="Synaptosomal-associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000018">
        <property type="entry name" value="Synaptosomal-associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000928">
        <property type="entry name" value="SNAP-25_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19305">
        <property type="entry name" value="SYNAPTOSOMAL ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19305:SF14">
        <property type="entry name" value="SYNAPTOSOMAL-ASSOCIATED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00835">
        <property type="entry name" value="SNAP-25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0770">Synapse</keyword>
    <keyword evidence="2" id="KW-0771">Synaptosome</keyword>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="9">
        <location>
            <begin position="27"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="9">
        <location>
            <begin position="149"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="58"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001394-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="213" mass="23550" checksum="2004B2188F5CC7FF" modified="2020-08-12" version="1">MPAAAPPAENGAAVPKTELQELQMKQQQVVDESLDSTRRMLALCEESKEAGIRTLVALDDQGEQLDRIEEGMDQINADMREAEKNLSGMEKCCGICVLPCNKSASFKEDDGTWKGNDDGKVVNNQPQRVMDDRNGLGPQAGYIGRITNDAREDEMEENMGQVNTMIGNLRNMALDMGSELENQNRQIDRINRKGDSNATRIAAANERAHDLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VFA6</accession>
    <name>A0A6E8VFA6_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Leucine-rich repeat protein soc-2 homolog</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_049461479.1">
        <property type="nucleotide sequence ID" value="XM_049605522.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VFA6"/>
    <dbReference type="EnsemblMetazoa" id="ACON002575-RB">
        <property type="protein sequence ID" value="ACON002575-PB"/>
        <property type="gene ID" value="ACON002575"/>
    </dbReference>
    <dbReference type="GeneID" id="120950179"/>
    <dbReference type="CTD" id="32156"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012757"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002575"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038389"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF1">
        <property type="entry name" value="RAS SUPPRESSOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52075">
        <property type="entry name" value="Outer arm dynein light chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002575-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="182" mass="20801" checksum="AE555B8CD80DA136" modified="2020-08-12" version="1">MASFVAKVVTRCEEALETKHLNLSECELIQVPDAVYHLMRHTELKTCDLSSNVITKISPKFAVKFSLITDLNLSHNQMARLPDELADLHSLEMLDISHNSFITLPAVVFKMPKLRELKANNNAIIDIDRDEIIASDSLELVDLRHNPLTPMCHDLLKHAVLSFRIELSERVKEDWEDLTECE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VFA7</accession>
    <name>A0A6E8VFA7_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Ion transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="22">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 17">Membrane</location>
            <topology evidence="1 17">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15 17">Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family.</text>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON002578-RB">
        <property type="protein sequence ID" value="ACON002578-PB"/>
        <property type="gene ID" value="ACON002578"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002304"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002578"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042804"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005891">
        <property type="term" value="C:voltage-gated calcium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008331">
        <property type="term" value="F:high voltage-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098703">
        <property type="term" value="P:calcium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009582">
        <property type="term" value="P:detection of abiotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009581">
        <property type="term" value="P:detection of external stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050906">
        <property type="term" value="P:detection of stimulus involved in sensory perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042045">
        <property type="term" value="P:epithelial fluid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016322">
        <property type="term" value="P:neuron remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000007">
        <property type="entry name" value="Voltage-dependent L-type calcium channel subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000001">
        <property type="entry name" value="Voltage-dependent L-type calcium channel subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000043">
        <property type="entry name" value="Voltage-dependent L-type calcium channel subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000059">
        <property type="entry name" value="Voltage-dependent N-type calcium channel subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000011">
        <property type="entry name" value="Voltage-dependent N-type calcium channel subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2500">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050599">
        <property type="entry name" value="VDCC_alpha-1_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002077">
        <property type="entry name" value="VDCCAlpha1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45628">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL TYPE A SUBUNIT ALPHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45628:SF7">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL TYPE A SUBUNIT ALPHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00167">
        <property type="entry name" value="CACHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 16 17" id="KW-0106">Calcium</keyword>
    <keyword evidence="4 17" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="3 17" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="18" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0407">Ion channel</keyword>
    <keyword evidence="11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="9 17" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="42"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="106"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="171"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="259"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="292"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="452"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="482"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="577"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="653"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="788"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="827"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="857"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="924"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="1038"/>
            <end position="1063"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="1119"/>
            <end position="1137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="1149"/>
            <end position="1171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="1183"/>
            <end position="1204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="1277"/>
            <end position="1295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="1316"/>
            <end position="1333"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="21">
        <location>
            <begin position="40"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="21">
        <location>
            <begin position="451"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="21">
        <location>
            <begin position="789"/>
            <end position="1068"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="21">
        <location>
            <begin position="1176"/>
            <end position="1329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="384"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="715"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="18">
        <location>
            <begin position="677"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="397"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="719"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="748"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="273"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="631"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="1009"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00061395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR602077-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002578-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1335" mass="152752" checksum="45C2C85CA83E8FE7" modified="2020-08-12" version="1">MGGPSSQGGQPLAGGGPTSLFILSEDNIIRKYTRFIIEWPPFEYAVLLTIIANCVVLALEEHLPHGDKTILAQKLEKTEAYFLGIFCVEASLKILALGFVMHKHSYLRNIWNIMDFFVVVTGFITLFPQKGPEVDLRTLRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGALHRSCYSLEDISQIVKEGEFPTPCNADNDTIAPTGAYVCNSSDSTCIEQWEGPNFGITSFDNIGFAMLTVFQCITMEGWTAILYWTNDALGSTFNWIYFVPLIVLGSFFMLNLVLGVLSGEFSNERGRVERQAQFHKCRSRQMFVEAMTSYLDWITQAEEVILAEERTTEEERLHIMEARRRAAAKRKKLKNLGKSKSTDTEEDDPDEDCGDDVFVPKRKKGYLKAKVKTQGKCKGFWRAEKRFRFWIRHTVKTQWFYWFVIVLVFFNTVCVAVEHYGQPNWLTQFLYYAEYVFLGLFMMEMWIKMYALGPRIYFESSFNRFDCVVISGSIFEVVWSEVKGGSFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLNSMRSIISLLFLLFLFILIFALLGMQLFGGQFNLPDGTPPTNFNTFPIALLTVFQILTGEDWNEVMYQGIESQGGHKKGMIYSLYFIILVLFGNYTLLNVFLAIAVDNLANAQELTAAEEEQLEENKEKQQMELDKEMEALHMQNDGSPPRVEVSSPSPTRGNGSGGSTKSKKKDEEKEEDDDEIPDGPKPMLPYSSMFVLSPTNPIRCGAHWVVNLRYFDFFIMVVISLSSIALAAEDPVEEDSPRNKILNFFDYAFTGVFTIEMLLKIVDLGVILHPGSYLREFWNVMDAVVVICAAVSFGFDMTGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVINILIVYILFQFIFAVIAVQLFNGKFFYCTDDSKHTSEECKGHFFVYDGADQLPRREPREWKTQSFHYDNVATAMLTLFAVQTGEGWPQVLQNSMAATYEDKGPIQNFRIEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQKSCIDFTIGARPLERYMPNKRNSFKYKVWRIVVSTPFEYFIMMLIVFNTLLLMMKYHNQGKEFEKSLKYLNMGFTGMFSVETILKIIGFGVKFHDAPTTLIDILSFMNLVFTFFFLLETIMKLIAYGCKNFFKDPWNIFDFITVIGSIIDALVLELGENSFNVGFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNMKFDPDTALNRHNNFQTFFGALLLLFRYLGILS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VFC3</accession>
    <name>A0A6E8VFC3_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VFC3"/>
    <dbReference type="EnsemblMetazoa" id="ACON002654-RA">
        <property type="protein sequence ID" value="ACON002654-PA"/>
        <property type="gene ID" value="ACON002654"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_008507"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002654"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030113"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000653">
        <property type="entry name" value="AGAP002654-PB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:001381">
        <property type="entry name" value="AGAP002654-PB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000892">
        <property type="entry name" value="AGAP002655-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24012">
        <property type="entry name" value="RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="38"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="204"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="291"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="422"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="383"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="394"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002654-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="715" mass="80737" checksum="E8B5C6E71A27F25A" modified="2020-08-12" version="1">MTDAEKTAPETTAETKETTKAENDGTEGDVQKDNEHQRTINVGNLPKDITEQQLRDHFAGHEVERVEIYHYLRNTLALLVFKEKAAAQKACEEKDGSMLNDRRLRIHIEYITIRYTKKDVIVAVLDDDMTEEKLYDRVKEHCEVTQVFIFHPLGYVHLGKDVDKAAVLEKLNAGGLKAYDVNGRDQNQHVDLWRAAKLFFRNRNRVQLLNIPQKWVENTEELKKACSEAGTITEAIANNVSQGSSNYYARIFFETEEQAAKAAELLNGKVFEGKRIHALHLSSALLPNYKTSVYVAPLERTVTEEEVYEHFKQFGDIDFVNRRNCGENAIVCYKTSEAAEKALACTTFPAPTEANKEATKTVTVLRYDGPLVLRATSVKRKAASGTAKGAAGEGKTRESRPAGGSDREKSHKDILQKLQAFYPVYVSNIPFSCPAHVIREFFSGAGGEVKFIFSPQHYHPYRLSSPQPVKTAMVYYTRRSDMLNAIKQLDKKMLNNQHLHVLPGRGDCNFSQQKTVKLSKITESLSEDAIFRKMRPLGKVVRLTKKNRSLAFVEFADAADVEKVLKMKPDELTINCVYSKITKDVSRRLYNESDPRILGTIVRLLRRNPKMLNKTTGAGPLMGGNSAVGGGPGGFGGGKRPRMNGPPGGKLFFLIDLLVCFTLHRLYFPVHFHNVEMHFCLLNSSTHSAFARRNSSISRYKFVYFLLLFFHSNFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VFC6</accession>
    <name>A0A6E8VFC6_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ZZ-type domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VFC6"/>
    <dbReference type="EnsemblMetazoa" id="ACON002670-RA">
        <property type="protein sequence ID" value="ACON002670-PA"/>
        <property type="gene ID" value="ACON002670"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002775"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002670"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031210"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="181"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="198"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="278"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="301"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="346"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002670-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="367" mass="37632" checksum="4F12B569F340C617" modified="2020-08-12" version="1">MYETIVVHFWRHPWALLLEVAFFCYTFLVLYASLFTTAARQQLERLPRRQQQPAVVSSAPNATGVNNNAIGATQANPNHTIITLNAGGRDGPIAASSSLPMVSTGVGGVCNVPGTVGGAAGAGSSAQQSQFLMARFMVPTMDDAEQTQLGRDRADRSQFVQALMLSTIANVQPFNKNAEEELSDELSSMNLGVGGGTNTCSSTVNSSVKRPASDDGGETAVGELLLQDGGATDSSNYGKEDGNCDSFKLGADSANGGSNDESPNMMHPSAAQHALGPQQQQQQQRGGHAGKSKSGSSTKSGLGGGGGAGGGGGGGGPVERRASRQTPPSGGGGANAAKARELKQANSSSNTSTSVSSRQQHHVPDSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VFE8</accession>
    <name>A0A6E8VFE8_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Dus domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <dbReference type="RefSeq" id="XP_040241693.2">
        <property type="nucleotide sequence ID" value="XM_040385759.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VFE8"/>
    <dbReference type="EnsemblMetazoa" id="ACON002791-RA">
        <property type="protein sequence ID" value="ACON002791-PA"/>
        <property type="gene ID" value="ACON002791"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON002791-RB">
        <property type="protein sequence ID" value="ACON002791-PB"/>
        <property type="gene ID" value="ACON002791"/>
    </dbReference>
    <dbReference type="GeneID" id="120961740"/>
    <dbReference type="CTD" id="35179"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007554"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002791"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038344"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017150">
        <property type="term" value="F:tRNA dihydrouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02801">
        <property type="entry name" value="DUS_like_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035587">
        <property type="entry name" value="DUS-like_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018517">
        <property type="entry name" value="tRNA_hU_synthase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11082">
        <property type="entry name" value="TRNA-DIHYDROURIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11082:SF31">
        <property type="entry name" value="TRNA-DIHYDROURIDINE(20A_20B) SYNTHASE [NAD(P)+]-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01207">
        <property type="entry name" value="Dus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01136">
        <property type="entry name" value="UPF0034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0288">FMN</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="DUS-like FMN-binding" evidence="6">
        <location>
            <begin position="1"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002791-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="362" mass="40264" checksum="212F8DF426CE85FB" modified="2020-08-12" version="1">MIMADSFCKSEKARLNEFTTNEEDTPLIAQFAANNTIDFLSASEMVYPYVDGVDLNCGCPQRWAMAEGYGSALLKTPELIADMLGTVRRNMPSTFSVSVKVRLLSSTDHKRTIDMCRQLEATGITFLTVHGRTAPEMTKVPVHKDALQEIKQSIGIPMVANGDIFTLDDAEQMYLQTKCDGVMTARGILSNPALFAGHSSTPVECVKRWLNICQQADTDITYQCMHHHFSFMTESLLTKRLRYELNNLSKEKQKVYEFIQTHLPLDGVIDDVGSGRQYPEKISCTYSERNYRERTTVVNGGRKDDSTAASAGSLYDPEASEGNFFQSKRVELDATTPALDGDGESTVDGVDFMDGSSILFDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VHE2</accession>
    <name>A0A6E8VHE2_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="24">Terribly reduced optic lobes</fullName>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="22">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endomembrane system</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
            <location evidence="2">Extracellular space</location>
            <location evidence="2">Extracellular matrix</location>
            <location evidence="2">Basement membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON003656-RA">
        <property type="protein sequence ID" value="ACON003656-PA"/>
        <property type="gene ID" value="ACON003656"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009102"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003656"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035234"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005604">
        <property type="term" value="C:basement membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00055">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002299">
        <property type="entry name" value="AGAP003656-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002808">
        <property type="entry name" value="AGAP003656-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000254">
        <property type="entry name" value="AGAP003656-PB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000236">
        <property type="entry name" value="AGAP003656-PC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000044">
        <property type="entry name" value="Basement membrane-specific heparan sulfate proteoglycan core protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000106">
        <property type="entry name" value="Heparan sulfate proteoglycan 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000105">
        <property type="entry name" value="Lipophorin receptor 1, isoform K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000034">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000062">
        <property type="entry name" value="Terribly reduced optic lobes, isoform AI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000086">
        <property type="entry name" value="Terribly reduced optic lobes, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000137">
        <property type="entry name" value="Uncharacterized protein, isoform AA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.300.10">
        <property type="entry name" value="Tie2 ligand-binding domain superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000034">
        <property type="entry name" value="Laminin_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050685">
        <property type="entry name" value="LDLR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002049">
        <property type="entry name" value="LE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056863">
        <property type="entry name" value="LMN_ATRN_NET-like_EGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24270">
        <property type="entry name" value="LOW-DENSITY LIPOPROTEIN RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24270:SF63">
        <property type="entry name" value="TERRIBLY REDUCED OPTIC LOBES, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00053">
        <property type="entry name" value="EGF_laminin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24973">
        <property type="entry name" value="EGF_LMN_ATRN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00052">
        <property type="entry name" value="Laminin_B"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00261">
        <property type="entry name" value="LDLRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00180">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SMART" id="SM00281">
        <property type="entry name" value="LamB"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01248">
        <property type="entry name" value="EGF_LAM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50027">
        <property type="entry name" value="EGF_LAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51115">
        <property type="entry name" value="LAMININ_IVA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="19"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0084">Basement membrane</keyword>
    <keyword evidence="12 16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14 16" id="KW-0424">Laminin EGF-like domain</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 18" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026326046" description="Terribly reduced optic lobes" evidence="18">
        <location>
            <begin position="26"/>
            <end position="2918"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="668"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="890"/>
            <end position="973"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin IV type A" evidence="21">
        <location>
            <begin position="1026"/>
            <end position="1211"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin IV type A" evidence="21">
        <location>
            <begin position="1393"/>
            <end position="1574"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="19">
        <location>
            <begin position="1608"/>
            <end position="1657"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin IV type A" evidence="21">
        <location>
            <begin position="1739"/>
            <end position="1908"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="2048"/>
            <end position="2136"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="2145"/>
            <end position="2225"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="2237"/>
            <end position="2327"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="2342"/>
            <end position="2423"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="2431"/>
            <end position="2517"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="2521"/>
            <end position="2608"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="2643"/>
            <end position="2720"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="2732"/>
            <end position="2818"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="2824"/>
            <end position="2913"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="2611"/>
            <end position="2646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="2612"/>
            <end position="2627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="17">
        <location>
            <begin position="2630"/>
            <end position="2644"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="28"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="35"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="47"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="129"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="146"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="153"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="165"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="186"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="193"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="227"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="234"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="246"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="273"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="280"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="292"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="330"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="348"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="355"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="367"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="388"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="395"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="407"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="450"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="478"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="490"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="507"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="514"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="526"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="543"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="550"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="562"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="581"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="588"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="600"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="618"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="625"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="770"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="777"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="789"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="810"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="817"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="829"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="853"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="860"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="872"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="1627"/>
            <end position="1636"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003656-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="24">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2918" mass="324138" checksum="859768D9F99680B9" modified="2020-08-12" version="1">MFSANSRAALWLAVLLVVSIERASGQECTSNEFTCDDGRCIDQDRQCDGVADCSRGEDEQDCGIVECDESREFLCRSDNTCIQKEAVCDGNRDCSDGEDEICEAQRDKCSIYEFQCRWDKRCIPIEKRCDKVYDCLDRTDEQVCDCTAHEFRCDNGFCIPMYQRCDRVNHCNDRSDERGCNFTSRCSEHEFHCNNGNCIPKDYMCNDIDDCGDNSDETDSVCGDYTCMPDEFTCYDGGCVSKALVCDDRKDCEDGTDEMDCDNNPPIKSLTRCGQGQFECNDGICIADYKHCNGIVDCHDESDETGCNSDPCSSNEFSCDGQCYEMQAYCNGVPDCSDGSDERNCAICYGDAFHCKNKQCILSVHICDGEPHCSDGSDELECNNNTNCKVFEWQCRNGFCINKDFRCDGSVDCTDRSDEEGCYDEERPSDPCREDEFYCDGRCFEEDRRCDGRADCTDGSDEHDCPTLPLERCEELQCPDGSCFRHSQRCDGVSDCADGYDEQNCPCRSNEFTCADGQCIPNYLLCNGRPDCADGSDERNCSCARNEFRCRTGQCIDELRRCDQRIDCVDGSDEKDCTRICRSNEWTCHSGQCIRLDQRCNRRYDCQDRSDEMSCTSCGPNKFRCENGPCIAMALKCNGRVDCPYDTSDELDCTDEFQYAPPPPFDQPRLNLKTYPDEQTIKESNEVVFQCRDEGPMHAKVKWVRGNGQPLPPGTRDMNGRLEIPNIRIDHNGEYICEAVGYPKSTPGSSKVVQLTVERFHYHQRPPTACGVNEATCMNGNCILKSQICDGRQDCADNSDENGCSHKDLCEPNQFKCRNRKCVLKTWLCDGEQDCGDGSDEENCATLPPNAPCRHDEFQCRDGQCIPKSFQCDTHPDCQDKSDEVGCMAPAVIQPPPPSLSIIAGGVLNITCRATGVPVPLIVWRLNWGHVPEKCTSRNDQGFGRLTCEDMQPIDSGAYSCEIINTMGTHFVSPDTIVIVTGSGPVCQQGTFNSQARNPSDCINCFCFGVTTSCNSADLYTYALKPPVSSLTIVGVEGPWSGRRELVVGEFENHNLTAQRHGVQLRLTNLVPGRRVPYYSLPEEYKGNQLKSYGGSIRYDVEYDGTGRPVNAPDVILKGNGMSLVHWHSGTFYPDIKNHVSVSLLPGNWVRPDRSPATREDIMMALASIESILIRMQYVDGIERNIELVNVMMDSAAHDDRGLGSASLVEQCRCPAGYRGLSCESCDYGYVRQNSGPWLGRCVKRERECQPGYYGDPNRDIPCAPCPCPVAGEKSRARSCYLDSRDNVVCQCDRGYTGERCMECEEGYVGNPLGEGCFPRPVTNCNALGTERIGSDRRCYCKPGIEGTYCDRCAAQHFYMHEKGCVECFCMGLTNVCSSTTWVRDTIHASFADGRSGFSLISDYTNPSIVANALPVSNREIVYRNFGASDDTFYWRLPNQFLGNKLTSFGGHLNYTLRYTPHSSGGISRNSSPDVVLHSGNKIKLHHYRTNSPISPYGSNTHSVSIVEDEWQNYEDGNAATREYLLMALANVSDIFIKATYNTVSNEAALSYVSLDIARDSPYATGARAWPVEQCQCPTGHIGLSCEDCAPGYYKGDQGIYLGICEPCACNGHSDECDPQTGACINCRDNTYGNNCELCRPPYVGNATVGSRYDCTLSQPDQSFDCRECDVRGFTGNCQRGCECKQLVEGRRCDQCREGSFGLGTQHETGCVECFCSGVTKSCGSSNLYREELPVIVDAYDNTITLTDREGTLLESRNFAINPSINEISYTFRDRETYYWSLPNLVLGNQVLSYGANLTVTQYVEGGRPLADQDIILIGSGMKLAWSREYYDDGTYAVPLVEHNWIVINRRNSYPASRGDMLTVLSNLEHILIRATTKESTSVSRLSDITLGTAIPTRTPYPAVEVEMCRCPPGYRGTSCEQCEDLHYRDIYDRSAGLLGACKPCPCNYENADSCQMDNRGVVVCNCREGYSGDNCDNRLPKRSRPANNRAPTKVDCTKNAFVFTSKNGIFNQYQKCDTEGVEIAVMYKSKRSNEELDTRPTVPPRNPVIEVVINVPSIKIVEIGEDFRADCTAHHVVSQSPIDVQWTRKNGRLPDSAYTDGGTLVITNIQIKDGGLYVCKASSGTEVVYKQVTINVSKQGSAKPTVLLSQPFIDQDEHLSAEVLCTATGYPTPTITWERVDQPLPYHAVTERGLLRFSALRQQDAGTYRCVARNDVGESDTLLTVYVRPAVGPTLPPSYPTEMERVEISPSSFDGQPGEVIRLICRCSPTAQVVWSKLGERQLPSNADVRNEMLIIEHASQENTGRYSCTAYFPNGRTKTSTVDVVIADNNVLPPSNKVAPRVNPLNKNYVVLQGADFSLSCEATGTPYPTVKWTLSGKTFESNVQQSGNVLRIFNAQPSNGGVYICVASNEEGMDRAYTVVEIDRREKPVLEIYPSEPQTIKVGESVRLSCRASAGVPYPSITWVRKDRMPLSSRFTNDAEGVITLRDASLEDAGEYECRADNAAGSAILSTTIEVLQPPIIRLQPSESHKITENDEFTIHCSATGKPAPMVTLLPPPGASRSHLRQQTEGLREVTLYLHQAELNDAGTYECTAKNAAGEDSQYLSLQVDPKRGDVGPHDDDDRSPSYYPPRLPPTRPPGPSSQPIAYTYKAILGEQAHLMCNEEFRSTRTEWRRSDGRPLPYGSIVRGGNLTIESTGHDAAGMYDCVTFPSPSQPVTIVRIMVEVISLPKISFSPQMPMTVRPGDAVDILCNVTSDVAYEVKWHRKDNLPLPPTVQAYGNYLRFSSIAPEDAGRYYCHVRNQFGETEKAAEVVVNKNELMPEPPIVGKRYHQASLGAAIRLDCKLPAGAPYVNVRYNWNRLERTLPPTARIVANGRELHLFNLEPEDAGTYQCGMSYPDGTVVYDSIVLTVGKYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VHI8</accession>
    <name>A0A6E8VHI8_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transcription initiation factor TFIID subunit 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040241004.2">
        <property type="nucleotide sequence ID" value="XM_040385070.2"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON003882-RB">
        <property type="protein sequence ID" value="ACON003882-PB"/>
        <property type="gene ID" value="ACON003882"/>
    </dbReference>
    <dbReference type="GeneID" id="120961361"/>
    <dbReference type="CTD" id="6872"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000851"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003882"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035102"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004402">
        <property type="term" value="F:histone acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05511">
        <property type="entry name" value="Bromo_TFIID"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1100.10:FF:000003">
        <property type="entry name" value="AGAP003882-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000039">
        <property type="entry name" value="Transcription initiation factor TFIID subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1100.10">
        <property type="entry name" value="TAFII-230 TBP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040240">
        <property type="entry name" value="TAF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022591">
        <property type="entry name" value="TAF1_HAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009067">
        <property type="entry name" value="TAF_II_230-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036741">
        <property type="entry name" value="TAFII-230_TBP-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041670">
        <property type="entry name" value="Znf-CCHC_6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13900">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13900:SF0">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12157">
        <property type="entry name" value="DUF3591"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09247">
        <property type="entry name" value="TBP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15288">
        <property type="entry name" value="zf-CCHC_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47055">
        <property type="entry name" value="TAF(II)230 TBP-binding fragment"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Bromo" evidence="11">
        <location>
            <begin position="1504"/>
            <end position="1574"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="11">
        <location>
            <begin position="1627"/>
            <end position="1697"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="58"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="112"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="158"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="258"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="332"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="453"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1019"/>
            <end position="1039"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1184"/>
            <end position="1210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1290"/>
            <end position="1365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1732"/>
            <end position="2003"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="1262"/>
            <end position="1289"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="1704"/>
            <end position="1731"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="135"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="159"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="195"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="214"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="467"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1196"/>
            <end position="1206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1290"/>
            <end position="1303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="1333"/>
            <end position="1347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="1735"/>
            <end position="1746"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="1810"/>
            <end position="1819"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1820"/>
            <end position="1841"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1857"/>
            <end position="1869"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1887"/>
            <end position="1922"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1933"/>
            <end position="1943"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1944"/>
            <end position="1968"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="1988"/>
            <end position="2003"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003882-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="2003" mass="225277" checksum="71C89D5DA68A203B" modified="2023-06-28" version="2">MSNSNSDNENEGGLNLAGFLFGNVDENGHLDGDFLDEEAKQQLSSLSRMGLSSFLADVLQDDDAEKPTRRPYSDSDDSSSSDDDRRRYDDDSDGANYKIKADCAVDYSDITELAEESAVPPPAVSKTEETDEAKEEDKKNIKVEDDIDDIEAAIPCTRVDARGTSDTTAVKEENAGGADGVDDKDLMPPPTAPVSADGTTGSTGTSTTATTTDDASKDKPKKLETPLAAMLPSKYQNVDVREFFPDFRPDKVLRFSRLFGPGKISSLPQIWRSVRRRRNKKSQSSSRKPRDGSDSTSDSDEPRRRQYFTPTYASLPPKDMWASDEELRFTSREADEEKEIKDKESDSKGDSKPKVADWRYGPAQKWYDMLDVPDTGEDFNYGFKQADPAKLEALPSVTSVTDNKAFPDDAFLMVSQLHWEDDVVWDGNDIKHKVLQKLNSKVNAAGWLPSSGSRTAGAFSQPGKTLPASPASTGSKGSSGSKMSKTQMIINAAQKAQEENEDAWYSIFPVENDELVYDKWEDDVIWDAEAVTKIPKPKVLTLDANDENIILCIPDDIDPSKIVRNTGPQPKVKIPHPHVKKSKILLGKAGVINVLAEDTPPPPPKSPDRDPYNISNDVFYMAKSTEATLKIKVGGGNLLQHSTPTVELRAPFVPTHMGPMKLRMFHRPPMKKYSYGSLASSNPQPVLPLQKHIKKKAKQRELERIASGGGDVFFMRTPEDLTGRDGELILIEFCEEHPPLMNQVGMASKIKNFYKRKMGKDPGPPEFRFGETHYAHTSPFLGILHHGQCIQAIENNMYRAPIYPHTIQETDFLVIRTRNNYFVREMDALFTAGQECPLYEVPGPNSKRANNFVRDFLQVFIYRLFWKSRDNPRKIRMDDIKKAFPAHSESSIRKRLKLCADFKRTGMDSNFWVIKPEFRLPSEEEIRAMVSPEQCCAYFSMIAAEQRLKDAGYGEKFIFAQQEDDDEEMQLKMDDEVKVAPWNTTRAYIQAMRGKCILQLNGPADPTGCGEGFSYVRMPNKPTQQNKEETETQPKRTVTGTDADLRRLSLNNAKALLRKFQVPEEEIKKLSRWEVIDVVRTLSTEKAKAGEEGMDKFSRGNRFSIAEHQERYKEECQRIFDLQNRVLASSEVLSTDEGESTASEESDLEELGKNLENMLANKKTSTQLSLEREEQERQELLRKIMEEQGGSGQKKKKDDEGMKDEQQSSTAKILKITRTFKNAEGREYTRVELVRRSPVIDAYVKIRTTKDEAFIRQFATLDEALKEEMKREKRRIQEQLRRIKRNQQKIGMQMQNPHSSVGTPISLGDRETNTPKPFANRMLDISGRSLDGSGAGPSGAGAAGTPGAGKEHSPSSSSRRKAKIKPDLKLKCGACGQVGHMRTNKACPQYSGILATPSLNVAMTEEQEEEIEKELNADDEGDLVNVDGTKVKLSSKLLKRHEDVKRRTLLLKVPKEAVGKKRRRVGGDSHCDYLQRHNKTTNRQRTDPVVVFSSVLEQVLNELRDMQDVQPFLFPVNAKQVVDYHKIVQRPMDLQTIRDNVRQKKYQTRDEFLMDINQIVENSALYNGAKSSLTVAAQRLLERCKESIQEREERLTRLEKSINPLLDDDDQVALSYILGEYVNGQLKSMPESWPFLKPVNKRLVKDYYTIIRRPMDLEKVSKKVASHKYHSRIDFLADLHLIADNSEQYNGAEANFTKQARQMVEAARQALDAMEHSVVHLERNIALVQERARNEDDDMDWEDDEQESKGDAGGGSRDTTPDLDDGGNDSNPDRPPSSTDASKAARAEAKRARARQRKAAKDDAGHQYSTDDEEFEEVGTSDNEGVSVTLEQSNTTSSMMPPQSEDDSQQAAEAMVQLSSAQYYNSAQDESMEIDPNYDPSDFLGMSSRQQTGDSGRNDAGIQQSSEASYSVDQNAPSSEYSQEAGRQEANDQQQQQQQFQYQPDPTGQSMDNPSQQPAALPTLQTLHSDLAISDSDDEKTNLQMDEVRDDNDDNDEGGDLWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VJ56</accession>
    <name>A0A6E8VJ56_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="EnsemblMetazoa" id="ACON004246-RA">
        <property type="protein sequence ID" value="ACON004246-PA"/>
        <property type="gene ID" value="ACON004246"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001195"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004246"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038935"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048666">
        <property type="term" value="P:neuron development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="CDD" id="cd14548">
        <property type="entry name" value="R3-PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12087">
        <property type="entry name" value="TM_EGFR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001137">
        <property type="entry name" value="Receptor protein-tyrosine phosphatase 10d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001460">
        <property type="entry name" value="Receptor protein-tyrosine phosphatase 10d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000916">
        <property type="entry name" value="Receptor-type tyrosine-protein phosphatase beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001177">
        <property type="entry name" value="Receptor-type tyrosine-protein phosphatase beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000009">
        <property type="entry name" value="Receptor-type tyrosine-protein phosphatase beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000823">
        <property type="entry name" value="Tyrosine-protein phosphatase 10D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001445">
        <property type="entry name" value="Tyrosine-protein phosphatase 10D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001589">
        <property type="entry name" value="Tyrosine-protein phosphatase 10D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001507">
        <property type="entry name" value="tyrosine-protein phosphatase 10D isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001334">
        <property type="entry name" value="tyrosine-protein phosphatase 10D isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001461">
        <property type="entry name" value="tyrosine-protein phosphatase 10D isoform X4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041201">
        <property type="entry name" value="PTPRJ_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050713">
        <property type="entry name" value="RTP_Phos/Ushers"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46957">
        <property type="entry name" value="CYTOKINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46957:SF3">
        <property type="entry name" value="CYTOKINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18861">
        <property type="entry name" value="PTP_tm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1222"/>
            <end position="1243"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="16">
        <location>
            <begin position="154"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="16">
        <location>
            <begin position="247"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="16">
        <location>
            <begin position="436"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="16">
        <location>
            <begin position="614"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="16">
        <location>
            <begin position="704"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="16">
        <location>
            <begin position="891"/>
            <end position="981"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="16">
        <location>
            <begin position="982"/>
            <end position="1081"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="14">
        <location>
            <begin position="1295"/>
            <end position="1550"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="15">
        <location>
            <begin position="1467"/>
            <end position="1541"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="20"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004246-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1591" mass="181286" checksum="8950ADC325AEB939" modified="2020-08-12" version="1">MQSTLERRKKMQPPTLHTHKQPERSDPATERRMGRRKLAAPGSMRLSSGLGLRELLLSSCLVVLLLAQQCYGADLVIEIPGNQGLDDSFYRLDYYPPIGNPAPNATIASRDVGDEIQFSNGLPGTRYNFWLYYTNSTHKDWLTWTVSITTAPDPPANLTVIPRSGKNVIINWSPPAQGNYSSFKLKILGLSDNFATNQTVAIEDNQFQYMVRDLTPGATYQVQAYTLYDGKESVAYTSRNFTTKPNTPGKFIVWFRNETTLLVLWQPPYPAGIYTHYKVSIEPPDALGSVLYVQKEGEPPGPAQAAFKGLVPGRAYNISVQTMSEDEISLPTTAQYRTVPLRPMNVTFDKKSITENSFKVMWEAPKGTSEFDKYQVSLSTSRRQQAVLRNDNENMAWLEFKDNLDPGKTYQVVVKTVSGKVTSWPASGDVTLKPLPVKQLQSYTDSKTGVITISWKPDELSTQDEYRISYHELETNNGDSSTMSTNQTSFALESLLPGRNYSVTVQALSRKMESNETVIFVVTRPSSPIIEDLKSIREGLNISWKSDVNSKQDKYEVTYTRNDTNDGKTVLTTESRLVFTNLYPGAGYEVKVFAVSHGLRSEPHSYFQAVYPNPPRNMTIEKVTSNSVLVHWKPPERSEFTEYSIRYRTESEKQWIRLPSVKATEADVTDMTPGEKYTIQVNTVSYGVESPNPQQVNQTVRPNPVSNIAPLVDSNNITLEFPRPEGRVETYIIHWWPTEQPEQVSMKNFTEVNTKPPLVRLLIGDLMSGVMYNFKIQTISYGLTSDLTKLQTRTMPLIQSEVVIVNNMHTRDMVTLSYTPTPQQSSKFDLYRFSLGDPSIPDKEKLANDTDRKVTFTGLTPGRLYNITVWTVSGKVSSQPIQRQDRMFPDPITMLEATSINDTWIALKWDIPKGEYTSFEVQYLMNDSHYVQNYTVNNHITITDLKPHRNYTITVVVRSGTESSVLRVSLPISANFQTKEALPGRMDKFAPIDIQPSEITFEWSLPPNEQNGIIRQFTITYGLDGSQHTQVKDFRPNELRGSIKALQPGKSYVFRIQAKTAIGYGPEHIWKQKMPILAPPKPETQVVPTEVGSSATTIEIRFRKHYFSDQNGVVTTYTIIIAEDDSKNASGLEMPSWRDVQSYSVWPPYQVIEPYYPFKNSSVEDFTIGTENCDAKKTGYCNGPLKSGTTYKVKVRAFTAPDKFTDTAYSYPIRTAQDNTSLIVSITVPLLIIAMLVGVVLFLRRRRHTGRKTTEQRTNDNMSLPDSTIETSRPVLVKNFAEHYRMMSADSDFRFSEEFEELKHIGRDQPCTFADLPCNRPKNRFTNILPYDHSRFKLQPVDDEEGSDYINANYVPGHNSPREFIVTQGPLHSTRDDFWRMCWESNSRAIVMLTRTFEKGREKCDHYWPHDTVPVYYGDIKVTLLNDSHYPDWVITEFMMTRGEQQRIIRHFHFTTWPDFGVPNPPQTLARFVRAFRERVGPDQRPIVVHCSAGVGRSGTFITLDRILQQIQVSDYVDIFGIVWAMRKERVWMVQTEQQYICIHQCLLVVLEGKEGTEREIHDNQGYEDESILNQTIYGHLSDSQVRLTCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VJJ1</accession>
    <name>A0A6E8VJJ1_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">RRM domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VJJ1"/>
    <dbReference type="EnsemblMetazoa" id="ACON004592-RA">
        <property type="protein sequence ID" value="ACON004592-PA"/>
        <property type="gene ID" value="ACON004592"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001593"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004592"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033712"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12337">
        <property type="entry name" value="RRM1_SRSF4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000420">
        <property type="entry name" value="serine-arginine protein 55 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050374">
        <property type="entry name" value="RRT5_SRSF_SR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23003">
        <property type="entry name" value="RNA RECOGNITION MOTIF RRM DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23003:SF51">
        <property type="entry name" value="SERINE-ARGININE PROTEIN 55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004592-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="132" mass="15238" checksum="2C1C161CECCFCCE5" modified="2023-06-28" version="2">MVGSRVYVGGLPYGVRERDLERFFKGYGRTRDILIKNGYGFVEFEDYRDADDAVYELNGKELLGERVVVEPARGTARGPSGYRERDRYDRDRRGGRYDKNSSRYGPPLRTEYRLVVENLSTRVSWQAAALIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VJJ2</accession>
    <name>A0A6E8VJJ2_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase</fullName>
            <ecNumber evidence="5">2.4.1.143</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Beta-1,2-N-acetylglucosaminyltransferase II</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">GlcNAc-T II</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Mannoside acetylglucosaminyltransferase 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase II</fullName>
        </alternativeName>
    </protein>
    <organism evidence="27 28">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="27">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>an N(4)-{beta-D-GlcNAc-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc}-L-asparaginyl-[protein] + UDP-N-acetyl-alpha-D-glucosamine = N(4)-{beta-D-GlcNAc-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[beta-D-GlcNAc-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc}-L-asparaginyl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12941"/>
            <dbReference type="Rhea" id="RHEA-COMP:13526"/>
            <dbReference type="Rhea" id="RHEA-COMP:14369"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:60615"/>
            <dbReference type="ChEBI" id="CHEBI:60651"/>
            <dbReference type="EC" id="2.4.1.143"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 24">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyltransferase 16 (GT16) protein family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.143" evidence="5"/>
    <dbReference type="RefSeq" id="XP_049462941.1">
        <property type="nucleotide sequence ID" value="XM_049606984.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VJJ2"/>
    <dbReference type="EnsemblMetazoa" id="ACON004397-RA">
        <property type="protein sequence ID" value="ACON004397-PA"/>
        <property type="gene ID" value="ACON004397"/>
    </dbReference>
    <dbReference type="GeneID" id="120951822"/>
    <dbReference type="CTD" id="4247"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001759"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004397"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032167"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005795">
        <property type="term" value="C:Golgi stack"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008455">
        <property type="term" value="F:alpha-1,6-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009312">
        <property type="term" value="P:oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007754">
        <property type="entry name" value="GlcNAc_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12871:SF0">
        <property type="entry name" value="ALPHA-1,6-MANNOSYL-GLYCOPROTEIN 2-BETA-N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12871">
        <property type="entry name" value="BETA-1,2-N-ACETYLGLUCOSAMINYLTRANSFERASE II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05060">
        <property type="entry name" value="MGAT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 25" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="17 24" id="KW-0464">Manganese</keyword>
    <keyword evidence="14 26" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 24" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 26" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 26" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="23"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <begin position="136"/>
            <end position="140"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <begin position="242"/>
            <end position="246"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="274"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="387"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
            <begin position="209"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
            <begin position="296"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
            <begin position="347"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
            <begin position="352"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
            <begin position="391"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00014817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00029663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00031203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00032552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00032915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00093257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PIRSR" id="PIRSR607754-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PIRSR" id="PIRSR607754-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PIRSR" id="PIRSR607754-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004397-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="464" mass="53962" checksum="47FA51397BDFAFCD" modified="2023-06-28" version="2">MRGRRIPIALPNTGALSRRRHTLYIRSAIIIFILFAFLQFHVINYDSSDSGSSDNANYSTAAILQMVPPVFHKYLTGKPRNSTHGQNGTHYAGDGPRTANISDIMRMINRYNDLQTVLNEDIYGPLQNDSVIIVVQVHKRITYLRHLIVSLAQARGISRTLLVFSHDYYDDDINDLVQSIDFCKVIQIFYPFSIQTHPTEFPGADPGDCPRDIKKEQALLRKCNNALHPDLYGHYREAKFTQTKHHWWWKANRVFDQLEVTKYHTGMMVFLEEDHYVAEDFLYILELMFRKSQDLCPKCNILSLGTYLKTFNYYTYNKKVEVTPWISSKHNMGMTFNRTTWYEIVRCARHFCEYDDYNWDWSLQHVSQQCLKQKLHVMVVKGPRVFHIGECGVHHKKSNCESNQVISKVQQVLKVARGAQQLFPRSISLTQTSVIKKTKLRKGNGGWGDQRDHQLCFNMTLVGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VJK9</accession>
    <name>A0A6E8VJK9_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="7">EF-hand domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_040218365.2">
        <property type="nucleotide sequence ID" value="XM_040362431.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VJK9"/>
    <dbReference type="EnsemblMetazoa" id="ACON004631-RA">
        <property type="protein sequence ID" value="ACON004631-PA"/>
        <property type="gene ID" value="ACON004631"/>
    </dbReference>
    <dbReference type="GeneID" id="120947261"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011009"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004631"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_036458"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052110">
        <property type="entry name" value="MCFD2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23104:SF17">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23104">
        <property type="entry name" value="MULTIPLE COAGULATION FACTOR DEFICIENCY PROTEIN 2 NEURAL STEM CELL DERIVED NEURONAL SURVIVAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039923045" evidence="5">
        <location>
            <begin position="22"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="123"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="32"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="46"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004631-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="217" mass="25419" checksum="2581B26E39923A4C" modified="2023-06-28" version="2">MKHSVLITLAILMQLASLAICQRVAPGVNPNHYQQQQQYQPPPPQYQQQAPQHQQPQYQQQPPQQQQQPQYQQQQFQQQQQHQQPHGEHGQQQVLHTGNLQQEKQHIAEHMEVPIDTSKMSEQELQFHYFKMHDSDNNNKLDGCELIKSLIHWHDNKDEHIGEMPSEGAPENHNDAQPYDDATLQSLVDPIMDMMDKDHDGFITYTEYRQHENAQNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VJL8</accession>
    <accession>A0A8W7Q041</accession>
    <name>A0A6E8VJL8_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Endocytosis/signaling protein ehd1</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_040218787.1">
        <property type="nucleotide sequence ID" value="XM_040362853.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VJL8"/>
    <dbReference type="EnsemblMetazoa" id="ACOM041032-RA">
        <property type="protein sequence ID" value="ACOM041032-PA"/>
        <property type="gene ID" value="ACOM041032"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON004593-RA">
        <property type="protein sequence ID" value="ACON004593-PA"/>
        <property type="gene ID" value="ACON004593"/>
    </dbReference>
    <dbReference type="GeneID" id="120947489"/>
    <dbReference type="CTD" id="41569"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007251"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004593"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030795"/>
    <dbReference type="Proteomes" id="UP000075882">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00052">
        <property type="entry name" value="EH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09913">
        <property type="entry name" value="EHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000147">
        <property type="entry name" value="EH domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000038">
        <property type="entry name" value="EH domain-containing protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.268.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040990">
        <property type="entry name" value="DUF5600"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045063">
        <property type="entry name" value="Dynamin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000261">
        <property type="entry name" value="EH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031692">
        <property type="entry name" value="EHD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030381">
        <property type="entry name" value="G_DYNAMIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11216:SF31">
        <property type="entry name" value="AT21416P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11216">
        <property type="entry name" value="EH DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18150">
        <property type="entry name" value="DUF5600"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00350">
        <property type="entry name" value="Dynamin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12763">
        <property type="entry name" value="EH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16880">
        <property type="entry name" value="EHD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00027">
        <property type="entry name" value="EH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50031">
        <property type="entry name" value="EH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51718">
        <property type="entry name" value="G_DYNAMIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0967">Endosome</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynamin-type G" evidence="13">
        <location>
            <begin position="52"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="EH" evidence="11">
        <location>
            <begin position="444"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="12">
        <location>
            <begin position="476"/>
            <end position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004593-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="534" mass="61390" checksum="77A898D687EAB400" modified="2023-06-28" version="2">MFSWLKRETKTEEVVENVLGELKKIYRSKLLPLEEHYNFHDFHSPKLEDSDFDAKPMILLVGQYSTGKTTFIRYLLERDFPGIRIGPEPTTDRFIAVMYDDKEGMIPGNALVVDPKKQFRPLEKYGNAFLNRFQCSHVPSPVLRAISIVDTPGILSGEKQRVDRGYDFTGVLEWFAERVDRIILLFDAHKLDISDEFRRSIEALRGHDDKIRIVLNKADMIDHQQLMRVYGALMWSLGKVLQTPEVARVYIGSFWDQPLRYDVNRRLFEDEEQDLFRDLQSLPRNAALRKLNDLIKRARLAKVHAYLISELRKEMPQIFGKDSKKKELIKNLGTIYDRVCREHQVSIGDLPDIKKMQEVLANQDFTKFHSLKMPLIEVVDRMLAVDIARLMSMIPQEEMQLVSEPLVKGGAFDGVEDVVSPFGYRKGEGIDAGYGEVDWICNRDRARTDPIFESLGPVDGKISGAAAKSELIKSKLPNNVLSKIWKLSDYDQDGFLDIEEFALAMHLINVKMDGNELPTALPLHLVPPSKRRVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VKX1</accession>
    <name>A0A6E8VKX1_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Guanine nucleotide-binding protein subunit alpha</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the G-alpha family. G(q) subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VKX1"/>
    <dbReference type="EnsemblMetazoa" id="ACON005079-RH">
        <property type="protein sequence ID" value="ACON005079-PH"/>
        <property type="gene ID" value="ACON005079"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON005079-RJ">
        <property type="protein sequence ID" value="ACON005079-PJ"/>
        <property type="gene ID" value="ACON005079"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007505"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005079"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032935"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005834">
        <property type="term" value="C:heterotrimeric G-protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001664">
        <property type="term" value="F:G protein-coupled receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031683">
        <property type="term" value="F:G-protein beta/gamma-subunit complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007188">
        <property type="term" value="P:adenylate cyclase-modulating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050793">
        <property type="term" value="P:regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00066">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.400.10:FF:000002">
        <property type="entry name" value="guanine nucleotide-binding protein G(Q) subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003977">
        <property type="entry name" value="Guanine nucleotide-binding protein G(q) subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000692">
        <property type="entry name" value="Guanine nucleotide-binding protein subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.400.10">
        <property type="entry name" value="GI Alpha 1, domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000654">
        <property type="entry name" value="Gprotein_alpha_Q"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001019">
        <property type="entry name" value="Gprotein_alpha_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011025">
        <property type="entry name" value="GproteinA_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218">
        <property type="entry name" value="GTP-BINDING PROTEIN ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218:SF329">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN G(Q) SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00503">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00318">
        <property type="entry name" value="GPROTEINA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00442">
        <property type="entry name" value="GPROTEINAQ"/>
    </dbReference>
    <dbReference type="SMART" id="SM00275">
        <property type="entry name" value="G_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47895">
        <property type="entry name" value="Transducin (alpha subunit), insertion domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51882">
        <property type="entry name" value="G_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9 11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4 10 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 9 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0564">Palmitate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0807">Transducer</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="43"/>
            <end position="48"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="149"/>
            <end position="150"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="174"/>
            <end position="180"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="199"/>
            <end position="203"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="268"/>
            <end position="271"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="325"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU369122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005079-PJ"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="353" mass="41813" checksum="7B9D8ECC3829807E" modified="2020-08-12" version="1">MECCLSEEAKEQKRINQEIERQLRRDKRDARRELKLLLLGTGESGKSTFIKQMRIIHGSGYSDEDKRGFIKLVYQNIFMAMQSMIRAMDLLKILYSDPANIEHAELIRSVDFETVTTFEPPYVQAIKDLWADAGIQECYDRRREYQLTDSAKYYLMEIDRVAAPNYLPTEQDILRVRVPTTGIIEYPFDLEEIRFRMVDVGGQRSERRKWIHCFENVTSIIFLVALSEYDQILFESENENRMEESKALFKTIITYPWFQHSSVILFLNKKDLLEEKIMYSHLVDYFPEYDGPKHDDVKAREFILKVYLRENPDPDRMCYSHFTCATDTENIRFVFAAVKDTILQSNLKEYNLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VKX3</accession>
    <name>A0A6E8VKX3_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 12">Ornithine aminotransferase</fullName>
            <ecNumber evidence="6 12">2.6.1.13</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a 2-oxocarboxylate + L-ornithine = L-glutamate 5-semialdehyde + an L-alpha-amino acid</text>
            <dbReference type="Rhea" id="RHEA:13877"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:58066"/>
            <dbReference type="ChEBI" id="CHEBI:59869"/>
            <dbReference type="EC" id="2.6.1.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 12">Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-ornithine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.13" evidence="6 12"/>
    <dbReference type="RefSeq" id="XP_040241238.2">
        <property type="nucleotide sequence ID" value="XM_040385304.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VKX3"/>
    <dbReference type="EnsemblMetazoa" id="ACON004793-RB">
        <property type="protein sequence ID" value="ACON004793-PB"/>
        <property type="gene ID" value="ACON004793"/>
    </dbReference>
    <dbReference type="GeneID" id="120961528"/>
    <dbReference type="CTD" id="4942"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_003657"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004793"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041139"/>
    <dbReference type="UniPathway" id="UPA00098">
        <property type="reaction ID" value="UER00358"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004587">
        <property type="term" value="F:ornithine aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019544">
        <property type="term" value="P:L-arginine catabolic process to L-glutamate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010121">
        <property type="term" value="P:L-arginine catabolic process to proline via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055129">
        <property type="term" value="P:L-proline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000011">
        <property type="entry name" value="Ornithine aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000152">
        <property type="entry name" value="Ornithine aminotransferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049704">
        <property type="entry name" value="Aminotrans_3_PPA_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050103">
        <property type="entry name" value="Class-III_PLP-dep_AT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010164">
        <property type="entry name" value="Orn_aminotrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01885">
        <property type="entry name" value="Orn_aminotrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11986">
        <property type="entry name" value="AMINOTRANSFERASE CLASS III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11986:SF18">
        <property type="entry name" value="ORNITHINE AMINOTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000521">
        <property type="entry name" value="Transaminase_4ab_Lys_Orn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="9 11" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU365036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004793-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="419" mass="45930" checksum="3140F02C38559E8F" modified="2020-08-12" version="1">MAKNGYGHAATALKAQPKSQAVFDREDKFGAHNYHPLPVALARGEGVYVWDVEGKRYYDFLSAYSAVNQGHCHPKIVQALTEQAQVLTLTSRAFYSNVLGEYEQFVTNLFGYDKVLPMNTGVEGGETACKLARKWGYKVKQIPENKAKIIFAEGNFWGRTLAAVSSSNDPSSYAGFGPFMPGFELIPYNDTAALEKALQDQHVCAFMVEPIQGEAGVVVPDEGYLRKVRELCSKYNVLFIADEVQTGLARTGRMLAVDHEDVKPDILILGKALSGGVYPVSAVLANDPVMLCIQPGEHGSTYGGNPLGCKVAIAALQVLVEEKLSENAERMGQLLRSSLRELPTDVVSIVRGKGLLNAIVINPNFDAWEVCLRLKENGLIAKPTHGDIIRFAPPLTINQEQLEDCLHIIKTTIMSFVQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VL97</accession>
    <name>A0A6E8VL97_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Aldedh domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040225148.1">
        <property type="nucleotide sequence ID" value="XM_040369214.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VL97"/>
    <dbReference type="EnsemblMetazoa" id="ACON005124-RB">
        <property type="protein sequence ID" value="ACON005124-PB"/>
        <property type="gene ID" value="ACON005124"/>
    </dbReference>
    <dbReference type="GeneID" id="120950851"/>
    <dbReference type="CTD" id="45398"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006213"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005124"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031069"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004029">
        <property type="term" value="F:aldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006081">
        <property type="term" value="P:aldehyde metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07132">
        <property type="entry name" value="ALDH_F3AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000003">
        <property type="entry name" value="Aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000004">
        <property type="entry name" value="Aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012394">
        <property type="entry name" value="Aldehyde_DH_NAD(P)"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43570">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43570:SF16">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE TYPE III, ISOFORM Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="2 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="584"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="8">
        <location>
            <begin position="120"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="27"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="50"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="61"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="71"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005124-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="609" mass="67093" checksum="9134BFDB89125A2F" modified="2020-08-12" version="1">MDKPKSALNIAEETRLFGEVPQVTLALSGTETSKPPSVKENVLKPSSDSIENHDGQHDTKSSGISSTTSSMKDDPSRDGQHLDESNLGQQPDSMTTVVIDIEATSGGSIVPDYINMSFSDIVQQLRTTFNSGKTRDVDFRLNQLKALLRCYEENTAEMANVLAADLRKHKQEAHLLEIEFILNDLRNTLYNLREWVKPEKPEKSLVNVMDGVYIYKDPYGVVLVIGAWNYPLQLTLVPVAAAIAAGNCVVIKPSEVAPASAKFIAETLPKYLSQDCYRVVTGGPKETSELLKEKFDYVFYTGSGRVGKIIHQACSENLTPCTLELGGKSPCYLDGTANIAIAARRILWGKFINAGQTCIAPDYILCSKQVQKQFLEEARKVLNEWYGANPKDSPDLCRIINQNHFQRLTSLLKGANVAIGGETDLQDRYISPTILIDVKSTDPVMQDEIFGPILPILNVDNAYDAIKFINSSAKPLCLYIFSKNVTDQRLMIENTSSGGVCVNDTLMHCAVEALPFGGVGPSGMGSYHGKYSFDTFVHKKSCLTKDFNPIGEKLAASRYPPYSESKLSFLSTLLKKRQGINLHFLPYLLMFGIGVASTLVVSTILKDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VLM9</accession>
    <name>A0A6E8VLM9_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Peptidoglycan recognition protein family domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VLM9"/>
    <dbReference type="EnsemblMetazoa" id="ACON005203-RE">
        <property type="protein sequence ID" value="ACON005203-PE"/>
        <property type="gene ID" value="ACON005203"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_014474"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005203"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033832"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008745">
        <property type="term" value="F:N-acetylmuramoyl-L-alanine amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009253">
        <property type="term" value="P:peptidoglycan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06583">
        <property type="entry name" value="PGRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.80.10:FF:000001">
        <property type="entry name" value="Peptidoglycan recognition protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.80.10">
        <property type="entry name" value="Peptidoglycan recognition protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036505">
        <property type="entry name" value="Amidase/PGRP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002502">
        <property type="entry name" value="Amidase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015510">
        <property type="entry name" value="PGRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006619">
        <property type="entry name" value="PGRP_domain_met/bac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11022">
        <property type="entry name" value="PEPTIDOGLYCAN RECOGNITION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11022:SF41">
        <property type="entry name" value="PEPTIDOGLYCAN-RECOGNITION PROTEIN LC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01510">
        <property type="entry name" value="Amidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00644">
        <property type="entry name" value="Ami_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00701">
        <property type="entry name" value="PGRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55846">
        <property type="entry name" value="N-acetylmuramoyl-L-alanine amidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0391">Immunity</keyword>
    <keyword evidence="2" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="218"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidoglycan recognition protein family" evidence="7">
        <location>
            <begin position="255"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetylmuramoyl-L-alanine amidase" evidence="6">
        <location>
            <begin position="267"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="171"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="8"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="50"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="171"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005203-PE"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="422" mass="45755" checksum="E373C75A7D0B41C2" modified="2020-08-12" version="1">MALARNLQLENDTSVNTGYHTKAQSMSASGGDTIAGLNNSGTGTGKSDSAKGSSLSNISASSIKRTDNGGDDDDSSVFDSSSSDTECDDDSIKRAIDRIPGTLAPGEARVLPNANVKVAVEAIAPGVRPSPAASTIGAIAVHNSSDITFGNKTYIKGQVVIKNIYQDRKNGTTNQGYQVQENETDDTNSTKKVPSATKPPEPRTWQSSLKTIIKDKPLLSFIVMVSLMIVLCAIVAVISILTASEDDLFPDPRPLRLVTRTEWLAQPPREELTDLKLPVNNVIIAHTATEGCTTQQACKALVQSIQRYHVNHTNYGDIGYNFLIGGDEFVYEGRGWLKVGAHTKNYNTISYGIAFIGNYETKDRPTEQQMEQLVLLLRNGTDGGWLAKEYKLCGASQLKGTISPGKYLMEQLRLLPHFSERL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VLN4</accession>
    <name>A0A6E8VLN4_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="13">BTB domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Putative transcription factor required for axon growth and guidance in the central and peripheral nervous systems. Repels CNS axons away from the midline by promoting the expression of the midline repellent sli and its receptor robo.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VLN4"/>
    <dbReference type="EnsemblMetazoa" id="ACON005245-RJ">
        <property type="protein sequence ID" value="ACON005245-PJ"/>
        <property type="gene ID" value="ACON005245"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009842"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005245"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_029613"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016199">
        <property type="term" value="P:axon midline choice point recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048813">
        <property type="term" value="P:dendrite morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008406">
        <property type="term" value="P:gonad development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035167">
        <property type="term" value="P:larval lymph gland hemopoiesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007526">
        <property type="term" value="P:larval somatic muscle development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045476">
        <property type="term" value="P:nurse cell apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007464">
        <property type="term" value="P:R3/R4 cell fate commitment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045467">
        <property type="term" value="P:R7 cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18315">
        <property type="entry name" value="BTB_POZ_BAB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000091">
        <property type="entry name" value="Lola, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051095">
        <property type="entry name" value="Dros_DevTransReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110">
        <property type="entry name" value="BTB DOMAIN TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110:SF111">
        <property type="entry name" value="LONGITUDINALS LACKING PROTEIN, ISOFORMS F_I_K_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3" id="KW-0221">Differentiation</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="11">
        <location>
            <begin position="32"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="575"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="115"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="301"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="446"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="161"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="175"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="212"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="322"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="460"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005245-PJ"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="605" mass="64707" checksum="EE2451DCFBE102D5" modified="2020-08-12" version="1">MDDDQQFCLRWNNHQSTLISVFDTLLENGTLVDCTLAAEGKLLKAHKVVLSACSPYFATILSQQYDKHPIFILKDVKFQELRAMMDYMYRGEVNISQDQLAALLKAAESLQIKGLSDNRSSSSSGTAAAGGGVHQKQSDAPAAKTLQPPVPASKASGLTIENKRSLKSELLESDVSGSREGSTSPTSRKRKKVRRRSVDTNNLLIDNHDQHSNSSSHSMHTAGGANVAAAVAAAGAPAANVASSLSSASSTAAAGSNTSANVLSVAAVAAAAVLKKTETVQQAAAETLKLQKQQAAAAAAAAAAQSSDDEERCGPGSVATGTEHDDGNDLSDNEHDGDGGSSVKSGSRHHHLRDGSRRGKTSTSSMGEMMIEPKSEYEDVQDENVEDLTMDDEDLMDELDQAGPSHGGDVSNGACTAQTGQERLQLKVVKCEENATITTPVALASEGFACDNGAPKEEKEQEEEEEEENVEDGDQMDVTLHRLGADEGILVPSASLFEDGHFGMAFTTAAGGSGAAHHYHHQPPSSLNDDLALMVGRPDDRGFFHCPADSCDRKYKIKYSLLRHLRNECNADRRYSCPKCKKKFSYAFILNRHLLNVHKESPGYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VLN9</accession>
    <name>A0A6E8VLN9_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="7">SERPIN domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the serpin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VLN9"/>
    <dbReference type="EnsemblMetazoa" id="ACON005246-RC">
        <property type="protein sequence ID" value="ACON005246-PC"/>
        <property type="gene ID" value="ACON005246"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011585"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005246"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038865"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19601">
        <property type="entry name" value="serpin42Da-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.497.10:FF:000006">
        <property type="entry name" value="Plasminogen activator inhibitor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.39.10">
        <property type="entry name" value="Alpha-1-antitrypsin, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.497.10">
        <property type="entry name" value="Antithrombin, subunit I, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023795">
        <property type="entry name" value="Serpin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023796">
        <property type="entry name" value="Serpin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000215">
        <property type="entry name" value="Serpin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036186">
        <property type="entry name" value="Serpin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042178">
        <property type="entry name" value="Serpin_sf_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042185">
        <property type="entry name" value="Serpin_sf_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461:SF211">
        <property type="entry name" value="GH10112P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461">
        <property type="entry name" value="SERINE PROTEASE INHIBITOR, SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00079">
        <property type="entry name" value="Serpin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00093">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56574">
        <property type="entry name" value="Serpins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00284">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0722">Serine protease inhibitor</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Serpin" evidence="6">
        <location>
            <begin position="21"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005246-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="380" mass="42550" checksum="832A1B7C6C7F5BD5" modified="2020-08-12" version="1">MADNSSSLDAQFVSQSNSFATKLYQRVSAKHAGENVVISPFSISACLSLAAMGAGGLTAEQMYSVLEFGAPDRKQTVADNYRRLMERLATDSTVNVANKIYVMQNYAVKGAFNAIATGSFRSEAESVNFAESAAAAKKINGWVEEKTNNKIKDLISPDALDELSRMVLVNAVHFKGTWTYQFDPSLTRPFPFWLSETESRDVPMMNIKKHFAFNNFEELGFSALELTYGGSDMTMMLLLPNERMGLAALEEKLPTLNLAELAGKMHKQEVEVFLPKFKIEFTRDLNEDLQALGMERMFSDSAEFPDLLEQNEPLKVSKVVHKAFIEVNEEGTEAAAATGMIMMMRCMPMHPYFTVDHPFLYVLRHQQMVYFVGRVAKIDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VLQ3</accession>
    <name>A0A6E8VLQ3_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="10">RIC3 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ric-3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VLQ3"/>
    <dbReference type="EnsemblMetazoa" id="ACON005057-RB">
        <property type="protein sequence ID" value="ACON005057-PB"/>
        <property type="gene ID" value="ACON005057"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005564"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005057"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037091"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034394">
        <property type="term" value="P:protein localization to cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007271">
        <property type="term" value="P:synaptic transmission, cholinergic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026160">
        <property type="entry name" value="Ric3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032763">
        <property type="entry name" value="RIC3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21723:SF3">
        <property type="entry name" value="PROTEIN RIC-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21723">
        <property type="entry name" value="RESISTANCE TO INHIBITORS OF CHOLINESTERASE PROTEIN 3 RIC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15361">
        <property type="entry name" value="RIC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="178"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="Resistance to inhibitors of cholinesterase protein 3 N-terminal" evidence="9">
        <location>
            <begin position="27"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="127"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="268"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="281"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="319"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF15361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005057-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="479" mass="52226" checksum="DBDCA2BE00888DC3" modified="2020-08-12" version="1">MATATGHRMESNSGMGPRKTMIIMVTVVGCVAILWPKVFYPMMVGNGQNKNVIKDHRGAGCCGVVLDQETFANASINYASSQQQQEQQNLFRKRNFAPYVDIDSIRQERPPHLRPEAMHPAMRERGRAIPQPGSIHGGDRPQSAPRIVEGRPGPIPGMRPPMGAGSHQATKSANSMGFIMPLYTIGIVSFFIYTIMKLIFKKTPATPYTEVKPDPVFRNEVFTTEQYIKRPDDGTTKLVVTAIQGLITAADEQLLKIDNIKKQAMNNASKETNVNGDEPATEEKETTSEVNATEDKVSNGHAIKPAENAAETTSAVQDTSGGETSSATSTPEKKPKKRDTSTERGTVKVMAMEVTAQRENGKRLSRPSTPLLPMSGHSDTANAPTEEQETRSIVLDGRLPHDSKILVSDAETAVEKMEPENSEEEDDAPVILSGKMTLSLINMDFIEKDATGTVAVGEIVTELHKPQEELATAVSKESE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VLQ8</accession>
    <name>A0A6E8VLQ8_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Small ribosomal subunit protein uS4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">40S ribosomal protein S9</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the small ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS4 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VLQ8"/>
    <dbReference type="EnsemblMetazoa" id="ACON005061-RA">
        <property type="protein sequence ID" value="ACON005061-PA"/>
        <property type="gene ID" value="ACON005061"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_013095"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005061"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_029836"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="entry name" value="RNA-binding S4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022801">
        <property type="entry name" value="Ribosomal_uS4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018079">
        <property type="entry name" value="Ribosomal_uS4_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005710">
        <property type="entry name" value="Ribosomal_uS4_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001912">
        <property type="entry name" value="Ribosomal_uS4_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003139">
        <property type="entry name" value="PRK04051.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01018">
        <property type="entry name" value="uS4_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831">
        <property type="entry name" value="30S 40S RIBOSOMAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831:SF5">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00163">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01390">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55174">
        <property type="entry name" value="Alpha-L RNA-binding motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00632">
        <property type="entry name" value="RIBOSOMAL_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS4 N-terminal" evidence="9">
        <location>
            <begin position="4"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00045441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005061-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="156" mass="18462" checksum="D0F284BF35E1993B" modified="2020-08-12" version="1">MVNHRIPSVFSKTYVTPRRPFEKPRLDAELKIIGQYGLRNKREVWRVKYTLAKIRKAARELLTLEEKDEKRLFQGNALLRRLVRIGVLDESRMKLDYVLGLRIEDFLERRLQTQVFKLGLAKSYHHARVLIRQRHIRSLERFCAGPVPPQMIVSAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VM24</accession>
    <name>A0A6E8VM24_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Diuretic hormone receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Receptor for the insect diurectic hormone. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VM24"/>
    <dbReference type="EnsemblMetazoa" id="ACON005464-RB">
        <property type="protein sequence ID" value="ACON005464-PB"/>
        <property type="gene ID" value="ACON005464"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007901"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005464"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040096"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008036">
        <property type="term" value="F:diuretic hormone receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008528">
        <property type="term" value="F:G protein-coupled peptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017046">
        <property type="term" value="F:peptide hormone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007188">
        <property type="term" value="P:adenylate cyclase-modulating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15263">
        <property type="entry name" value="7tmB1_DH_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000155">
        <property type="entry name" value="diuretic hormone receptor isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1240.10">
        <property type="entry name" value="GPCR, family 2, extracellular hormone receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050332">
        <property type="entry name" value="GPCR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002001">
        <property type="entry name" value="GPCR_2_diuretic_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036445">
        <property type="entry name" value="GPCR_2_extracell_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001879">
        <property type="entry name" value="GPCR_2_extracellular_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017983">
        <property type="entry name" value="GPCR_2_secretin-like_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45620:SF15">
        <property type="entry name" value="DIURETIC HORMONE 44 RECEPTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45620">
        <property type="entry name" value="PDF RECEPTOR-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02793">
        <property type="entry name" value="HRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01127">
        <property type="entry name" value="DIUHORMONER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00249">
        <property type="entry name" value="GPCRSECRETIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00008">
        <property type="entry name" value="HormR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111418">
        <property type="entry name" value="Hormone receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00649">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00650">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50227">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="151"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="186"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="225"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="258"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="307"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="353"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="378"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 1" evidence="14">
        <location>
            <begin position="57"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="15">
        <location>
            <begin position="149"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00054836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00071387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005464-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="444" mass="49681" checksum="E25CB7D88EAD2C93" modified="2020-08-12" version="1">MANEIMPATPTAAAIAADDGSGGSPFGEANVTGISVENPSLVEGLLALAMNASGAERCRLQQQAEELLQDVACPSFFDMVSCWPRTPPGTLAVLPCFAELKGVQYDSSQNATRFCNVDGTWDNYTDYDRCEHLEQPPPLPSFEPEIELPTLIYFVGYSISLAALVLAVAVLVYFKDLRCLRNTIHVNLFLTYIMSSSLWILILSLQITVKLEVAGCIFLVTLFHYFSTTNFFWMLVEGLYLYMLVVQTFSGDTLRFRKYAIIGWGGPLIFVGAWAIAKPFFGSVSNLEHPSKLEIECSWMRESHIDWIIQGPSCAVLVINLIFLLRIMWVLITKLRSANTVETRQYRKASKALLVLIPLLGITYLIVIYGPVEGVGSHIFAITRAILLSTQGFVVSLLYCFLNSEVRQTLRHHFYRWRDERNILSGKVNNHHRSLTTTTSAPLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VNP3</accession>
    <name>A0A6E8VNP3_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Purine nucleoside phosphorylase</fullName>
            <ecNumber evidence="3 11">2.4.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Inosine-guanosine phosphorylase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">The purine nucleoside phosphorylases catalyze the phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>2'-deoxyguanosine + phosphate = 2-deoxy-alpha-D-ribose 1-phosphate + guanine</text>
            <dbReference type="Rhea" id="RHEA:27738"/>
            <dbReference type="ChEBI" id="CHEBI:16235"/>
            <dbReference type="ChEBI" id="CHEBI:17172"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57259"/>
            <dbReference type="EC" id="2.4.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>2'-deoxyinosine + phosphate = 2-deoxy-alpha-D-ribose 1-phosphate + hypoxanthine</text>
            <dbReference type="Rhea" id="RHEA:27750"/>
            <dbReference type="ChEBI" id="CHEBI:17368"/>
            <dbReference type="ChEBI" id="CHEBI:28997"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57259"/>
            <dbReference type="EC" id="2.4.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>guanosine + phosphate = alpha-D-ribose 1-phosphate + guanine</text>
            <dbReference type="Rhea" id="RHEA:13233"/>
            <dbReference type="ChEBI" id="CHEBI:16235"/>
            <dbReference type="ChEBI" id="CHEBI:16750"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57720"/>
            <dbReference type="EC" id="2.4.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>inosine + phosphate = alpha-D-ribose 1-phosphate + hypoxanthine</text>
            <dbReference type="Rhea" id="RHEA:27646"/>
            <dbReference type="ChEBI" id="CHEBI:17368"/>
            <dbReference type="ChEBI" id="CHEBI:17596"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57720"/>
            <dbReference type="EC" id="2.4.2.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Purine metabolism; purine nucleoside salvage.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the PNP/MTAP phosphorylase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.1" evidence="3 11"/>
    <dbReference type="RefSeq" id="XP_040221858.1">
        <property type="nucleotide sequence ID" value="XM_040365924.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VNP3"/>
    <dbReference type="EnsemblMetazoa" id="ACON005945-RA">
        <property type="protein sequence ID" value="ACON005945-PA"/>
        <property type="gene ID" value="ACON005945"/>
    </dbReference>
    <dbReference type="GeneID" id="120949002"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002620"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005945"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042586"/>
    <dbReference type="UniPathway" id="UPA00606"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004731">
        <property type="term" value="F:purine-nucleoside phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009116">
        <property type="term" value="P:nucleoside metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09009">
        <property type="entry name" value="PNP-EcPNPII_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1580:FF:000004">
        <property type="entry name" value="Purine nucleoside phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1580">
        <property type="entry name" value="Nucleoside phosphorylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000845">
        <property type="entry name" value="Nucleoside_phosphorylase_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035994">
        <property type="entry name" value="Nucleoside_phosphorylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011270">
        <property type="entry name" value="Pur_Nuc_Pase_Ino/Guo-sp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011268">
        <property type="entry name" value="Purine_phosphorylase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01700">
        <property type="entry name" value="PNPH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01697">
        <property type="entry name" value="PNPH-PUNA-XAPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006054">
        <property type="entry name" value="PRK08202.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11904">
        <property type="entry name" value="METHYLTHIOADENOSINE/PURINE NUCLEOSIDE PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11904:SF9">
        <property type="entry name" value="PURINE NUCLEOSIDE PHOSPHORYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01048">
        <property type="entry name" value="PNP_UDP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000477">
        <property type="entry name" value="PurNPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53167">
        <property type="entry name" value="Purine and uridine phosphorylases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleoside phosphorylase" evidence="13">
        <location>
            <begin position="39"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="97"/>
            <end position="99"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>a purine D-ribonucleoside</name>
            <dbReference type="ChEBI" id="CHEBI:142355"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>a purine D-ribonucleoside</name>
            <dbReference type="ChEBI" id="CHEBI:142355"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR000477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000477-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005945-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="301" mass="33178" checksum="C80A14AF6C0365E0" modified="2020-08-12" version="1">MAAFIESKQSGGGSSRMYTYDTLQEIATYLLERTELRPKVGIICGSGLGTLAEQLTDVDSFDYETIPHFPVSTVAGHVGRLVFGYLAGVPVMCMQGRFHHYEGYPLAKCAMPVRVMHLIGCTHLIATNAAGGANPKYRVGDIMLIKDHINLMGFAGNNPLQGPNDERFGPRFFGMANTYDPKLNQQAKVIARQIGIENELREGVYTCLGGPNFETVAEVKMLSMLGVDAIGMSTVHEIITARHCGMTCFAFSLITNMCTMSYEEEEEHCHDSIVGVGKNREKTLGEFVSRIVKHIHYEAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VNP8</accession>
    <name>A0A6E8VNP8_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase</fullName>
            <ecNumber evidence="3">3.2.2.6</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>NAD(+) + H2O = ADP-D-ribose + nicotinamide + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16301"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57967"/>
            <dbReference type="EC" id="3.2.2.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:16302"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SARM1 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.6" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8VNP8"/>
    <dbReference type="EnsemblMetazoa" id="ACON005901-RB">
        <property type="protein sequence ID" value="ACON005901-PB"/>
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        <property type="component" value="Unplaced"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:NAD+ nucleosidase activity"/>
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        <property type="term" value="F:NAD+ nucleosidase activity, cyclic ADP-ribose generating"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Ectoderm-expressed 4, isoform D"/>
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        <property type="entry name" value="Sterile alpha and TIR motif-containing 1"/>
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        <property type="entry name" value="Leucine-rich Repeat Variant"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0391">Immunity</keyword>
    <keyword evidence="5" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="9" id="KW-0520">NAD</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="SAM" evidence="13">
        <location>
            <begin position="690"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="domain" description="TIR" evidence="12">
        <location>
            <begin position="837"/>
            <end position="980"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="288"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="997"/>
            <end position="1031"/>
        </location>
    </feature>
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            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
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        <location>
            <begin position="41"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="294"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1014"/>
            <end position="1031"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008291"/>
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            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022588"/>
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    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022859"/>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1031" mass="113615" checksum="9777B745FF689227" modified="2020-08-12" version="1">MSSLTSSSSSSSSTTSAAAVKQGLGEMQNSIAEMKNMMGHSSSSSSSTSQNLSTTTSTSSIKSSALSNHRKMSSKINDIKSRLRSSMDSLDDPQGADPLVTFPDSETDDDHLSGMASSLKPLGANGGGSGSILVPNGTNHHHHQALMNGCAAAGTTVDTVKFEQKKMTSESKTKVTTDGFSSEQATINSAESKKLQAGDLQYQEQAALAATSSRMEVDGVTAEEKGAILKEARSLKMGDFQQAEANNIAAHSRKVHSDHFSAENKAISQAQQKQTVSASGIFNQEKHVSSTTQSTFTMSNTSTISSSSQMSTLSSSSSAALGDDFPSFEDFERLGNTPEEIDSTIMKYSKHLANYVQNLQQTEQLKKAPTLLNHMNEIIRRAWAVPTHGHELGASLCDTLRNTGGLDILMRNCVDNDSEVQFSSARLLEQCLTTENRTHVVKNGLDKVVNVACVCTRNNATDHSRVGTGILEHLFKHSEDTCSNVIRLGGLDAVLFECRKNDVETLRHCAGALANLSLYGGSENQEAMINRKVPSWLFPLAFHNDENIKYYACLAIVVLVANKEIEAEVIKSGTLNLVEPFVTTHNPSEFAKSNLAHAHGQSKHWLQRLVPVLSSKREEARNLAAFHFCMEAGIKKEQGNTNIFKEIGAIEPLIRVASSPNAIASKFAAQALRLIGEEVPHKLSQQVPLWSTEDVREWVKQIGFGEYEQSFYDSKVDGDLLLQMQDHNLCDDIGMKNGILRIRFLRELQNLKKMADYSSRDTDGLHAFLSRIGPEYTIYTYTMLNAGVDIESLRTLDEDQLTATCGITNAIHRSRILQVIKLNESIPSFRSEQSAEKNLDVFVSYRRSNGSQLASLLKVHLELRGFSVFIDVERLEAGKFDNNLLQSIRHARHFLLVLTPDALNRCIDDVDCKDWVHREIVAALNSECNIIPIIDNFQWPEPEKLPEDMRGVCHFNGIRWIHDYQDACVDKVERFMRAEMNGRGGCGLLDHGSRLLSRQPGDATPTSAIYQRMHSNDSGKSSDKENSRNLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VNS6</accession>
    <name>A0A6E8VNS6_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DUF4774 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VNS6"/>
    <dbReference type="EnsemblMetazoa" id="ACON005613-RA">
        <property type="protein sequence ID" value="ACON005613-PA"/>
        <property type="gene ID" value="ACON005613"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001571"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005613"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038158"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039951372" evidence="2">
        <location>
            <begin position="21"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="113"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="164"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="228"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="177"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="193"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="228"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="260"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005613-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="285" mass="30448" checksum="C994F0BDC04DE555" modified="2023-06-28" version="2">MSFSSVAVLALLCGVGLCAAYPYPYPQNAFMYYRNALPVMAYPQLQQTAMYQHAMYQNQRLRQNYQRRSATSGVAAFAAGDNIATGTILKDPAVEGYPSVQSFADAYPADALPENEIPLSDDGGQLEQDGNAGGELDFALDNEEPLEDVPAVAAAPLVPTTTDKRKKKVTVQLDSASAEDDQEDEEQVSFGTRRGGSAGRPAGGSAGPMFPVTFGSTNGGAIAIANSYSTGKGGSATSHATAYGSPASVPEERRRAVVLQQQKQHQQQQQQQQRSIRPAKLRTKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VNU1</accession>
    <name>A0A6E8VNU1_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Molybdenum cofactor biosynthesis protein 1</fullName>
            <ecNumber evidence="6">4.1.99.22</ecNumber>
            <ecNumber evidence="7">4.6.1.17</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="23">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="19">Isoform MOCS1A and isoform MOCS1B probably form a complex that catalyzes the conversion of 5'-GTP to cyclic pyranopterin monophosphate (cPMP). MOCS1A catalyzes the cyclization of GTP to (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate and MOCS1B catalyzes the subsequent conversion of (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate to cPMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate = cyclic pyranopterin phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:49580"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:59648"/>
            <dbReference type="ChEBI" id="CHEBI:131766"/>
            <dbReference type="EC" id="4.6.1.17"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>GTP + AH2 + S-adenosyl-L-methionine = (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + L-methionine + A + H(+)</text>
            <dbReference type="Rhea" id="RHEA:49576"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:131766"/>
            <dbReference type="EC" id="4.1.99.22"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="20">Isoform MOCS1A and isoform MOCS1B probably form a heterooligomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the C-terminal section; belongs to the MoaC family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">In the N-terminal section; belongs to the radical SAM superfamily. MoaA family.</text>
    </comment>
    <dbReference type="EC" id="4.1.99.22" evidence="6"/>
    <dbReference type="EC" id="4.6.1.17" evidence="7"/>
    <dbReference type="RefSeq" id="XP_049462873.1">
        <property type="nucleotide sequence ID" value="XM_049606916.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VNU1"/>
    <dbReference type="EnsemblMetazoa" id="ACON006072-RA">
        <property type="protein sequence ID" value="ACON006072-PA"/>
        <property type="gene ID" value="ACON006072"/>
    </dbReference>
    <dbReference type="GeneID" id="120948367"/>
    <dbReference type="CTD" id="4337"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004500"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON006072"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_043223"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061799">
        <property type="term" value="F:cyclic pyranopterin monophosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061798">
        <property type="term" value="F:GTP 3',8'-cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21117">
        <property type="entry name" value="Twitch_MoaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000117">
        <property type="entry name" value="molybdenum cofactor biosynthesis protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01225_B">
        <property type="entry name" value="MoaA_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013483">
        <property type="entry name" value="MoaA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040064">
        <property type="entry name" value="MoaA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000385">
        <property type="entry name" value="MoaA_NifB_PqqE_Fe-S-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010505">
        <property type="entry name" value="MoaA_twitch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050105">
        <property type="entry name" value="MoCo_biosynth_MoaA/MoaC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02666">
        <property type="entry name" value="moaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22960:SF0">
        <property type="entry name" value="MOLYBDENUM COFACTOR BIOSYNTHESIS PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22960">
        <property type="entry name" value="MOLYBDOPTERIN COFACTOR SYNTHESIS PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06463">
        <property type="entry name" value="Mob_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01383">
        <property type="entry name" value="cyclic_pyranopterin_phosphate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01386">
        <property type="entry name" value="main_SPASM_domain-containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01067">
        <property type="entry name" value="SPASM/twitch_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01305">
        <property type="entry name" value="MOAA_NIFB_PQQE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="15" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="13" id="KW-0408">Iron</keyword>
    <keyword evidence="14" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="17" id="KW-0456">Lyase</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="22">
        <location>
            <begin position="95"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="35"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="51"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="67"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00015273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00054222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00063038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON006072-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="417" mass="46059" checksum="0B669DFFFD365858" modified="2020-08-12" version="1">MHRLMVRGVVRGAAASIDSSSRWVLPGPKLLHTARPCLSDAGSSGDRRTQSPETSASSAAAPSTVDSADKNKSNSERERSQPTTAAATASTLSDSFGRFHSYLRISLTERCNLRCKYCMPADGVQLTQKQQLLTSAEVLQLASLFVAEGVRKIRLTGGEPTVRKDLTEIVAQLKATNPLLESVGITTNGLMLTRQLVGLQRAGLDALNVSLDTLRAARYEQITRRKGWDRVMAGIDLAIQLGYRPKVNCVLMKGFNEDEICDFVELTRDRNVDVRFIEYMPFTGNRWDTDKMVPYRAMLETIRARYPAFEALPNGPNDTSKAYRVPGYRGQMGFITSMTEHFCGSCNRLRITADGNLKVCLFGNTEVSLRDALRSPGCSEDDLRCLISAAVKRKKKQHAGMLNLSQMENRPMILIGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VPU4</accession>
    <name>A0A6E8VPU4_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8VPU4"/>
    <dbReference type="EnsemblMetazoa" id="ACON006340-RB">
        <property type="protein sequence ID" value="ACON006340-PB"/>
        <property type="gene ID" value="ACON006340"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012613"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON006340"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037044"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06608">
        <property type="entry name" value="STKc_myosinIII_N_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000003">
        <property type="entry name" value="TRAF2 and NCK-interacting protein kinase isoform 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000006">
        <property type="entry name" value="TRAF2 and NCK-interacting protein kinase isoform 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001180">
        <property type="entry name" value="CNH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051700">
        <property type="entry name" value="STE20_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47096">
        <property type="entry name" value="MISSHAPEN LIKE KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47096:SF1">
        <property type="entry name" value="MISSHAPEN LIKE KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00780">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00036">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50219">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="21"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="CNH" evidence="13">
        <location>
            <begin position="869"/>
            <end position="1160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="304"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="358"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="742"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="832"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="364"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="386"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="422"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="488"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="506"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="523"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="553"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="625"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="663"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="692"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="742"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="788"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="843"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON006340-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1186" mass="130636" checksum="109BBCF87C2317FB" modified="2020-08-12" version="1">MWFTDILLSFSLSLQDPAGIFELIEVVGNGTYGQVYKGRHTKTGQLAAIKVMDVTEEEEEEIKLEINVLKKYSNHRNIATYYGAFIKKTPAGKDDQLWLVMEYCGAGSVTDLVKSTKGQSLKEEWIAYICREILRGLSYLHTNKVIHRDIKGQNVLLTDNAEVKLVDFGVSAQLDKTIGRRNTFIGTPYWMAPEVIACDENRDATYDNRSDLWSLGITALEMAESQPPLCDLHPMRALFLIPRNPPPRMKSKKWSKKFHSFIDTVLVKDYHQRPYTEQLLKHPFIKEQPTERQVRIQLKDHIDRCKKRKQEKERDDYRYSGSENDDDDGQTAGEPSSIIQAPGNDTLRRTFHQIQEGRMQNAEQQQPPNRNQKPQPRDERSKPQPVEEPGPPSRPQLPQRLIVVPDPPANSNANRPLPPTPRSGSGSSSQPQQPSSQTPQQPARNQQNFFKPVVSSGRATPLYTQSQQAQPEAPPRNNRPQPGPPVPGSQQQQQQVSAGGVGASGVGKAPAIAPNGNNNSSNGAGGGSGGGVGGNINNNNHHPQPINPLDPIESSDSDSEPEEPNGRTRNDGTLLASDPPMPLPEFSYRTGGLGVLAESDQNNQSSSSTPAGGGGASGASVLSPPGGGGGGGGGPPNRPLPPTPDDDDAQGDGTLIKRKNRSGENSSLGTPGQRTSSVLPDLLSQASPATPPRHDKSASEEYRAAVNASVHPNHSINTRLLSSSSNSSSSAVVVNHKLLNNNHLLPPPYHQQYHQPSPHHHQMQHHLPMGTMPVHLLPGANGNANHHQQSSGAAQQPSPQQQQQPLPVSPFSLALQQKQRSFLTFGFGAQSGGSAASRRESHVNVNVTPTSHDATSDTPEIRKYKKRFNSEILCAALWGVNLLIGTENGLMLLDRSGQGKVYQLISRRRFQQMEVLEGQNILVTISGKKNRVRVYYLSWLKSKILRTDGLTDQQVERRNGWINVGDLQGAVHFKIVKYERIKFLVIALKDSIEIYAWAPKPYHKFMAFKSFGELMHRPLLVDLTVEEQTRLKVIYGSAEGFHAVDLDSATVYDIYLPKHTQGPISPHCIVTLPNSNGMQLLLCYDNEGVYVNTMGRVSKNIVLQWGEMPTSVAYIGTGQIMGWGNKAIEIRSVETGHLDGVFMHKKAQRLKFLCERNDKVFFSSAKGGSSCQIYFMTLNKPGMANW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VPV1</accession>
    <name>A0A6E8VPV1_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF4766 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_040225778.2">
        <property type="nucleotide sequence ID" value="XM_040369844.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VPV1"/>
    <dbReference type="EnsemblMetazoa" id="ACON006410-RB">
        <property type="protein sequence ID" value="ACON006410-PB"/>
        <property type="gene ID" value="ACON006410"/>
    </dbReference>
    <dbReference type="GeneID" id="120951250"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_014520"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON006410"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038247"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026191901" description="DUF4766 domain-containing protein" evidence="2">
        <location>
            <begin position="22"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="208"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON006410-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="276" mass="28001" checksum="F602B9F599DF3C0A" modified="2020-08-12" version="1">MKPTTSSIALLILVIVQCTLASPVPQFLTFRDGKFGVNFGGYHAEAGLGGLLTGNSAHGGLSASAGTPHGQQAGAGIGGLLGGNERTAGGAYAGATAGHGVGASAAIGGGLDGAGGAGGAGAESHAGGVSKKVVRLGQTNQGAPPTEVVISKETGAHHQGNFERIDDHHVKEVHTELRAPAPQPAPEHSFRKTIYKKKVISHPHKIHTRIDSSNGHSGGHSGASSSTHVVASSTHNSNFWNDIFNIPISTLTAVNQLLNNKAGSGSLHVEKRIEHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VPV3</accession>
    <name>A0A6E8VPV3_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="10">C2H2-type domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VPV3"/>
    <dbReference type="EnsemblMetazoa" id="ACON005978-RA">
        <property type="protein sequence ID" value="ACON005978-PA"/>
        <property type="gene ID" value="ACON005978"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006917"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005978"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038787"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000383">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000250">
        <property type="entry name" value="zinc finger protein 197 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001272">
        <property type="entry name" value="Zinc finger protein 683"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050331">
        <property type="entry name" value="Zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515">
        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515:SF58">
        <property type="entry name" value="ZINC FINGER PROTEIN 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="49"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="77"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="105"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="133"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="161"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="189"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="217"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="245"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005978-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="300" mass="34762" checksum="F1918030DEB3A076" modified="2020-08-12" version="1">MFYPLQTQFDDGVIELTRSEMLQQDHLVDNKHFVVSLSLGNTLINLNKIKCPQCRKRFDTMEEMEQHRTKHLTENKFKCEICSKEFPSHSSMWKHTKAHTGERPFVCQICNKGFTQLANLQRHDLVHNGLKPFKCPICEKCFTQQANMLKHQLLHTGLKPYKCPVCEKAFSQHANMVKHQMLHTGLKPYKCPVCEKAFTQHANMIKHQMLHTGLKPYKCPVCDKAFTQQANMVKHQMLHTGLKPYKCSTCGKAFAQQANMVKHEMLHTGNSIECFYIDTIMKQEDDDDDDTREIESDSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VPY6</accession>
    <name>A0A6E8VPY6_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_040226228.2">
        <property type="nucleotide sequence ID" value="XM_040370294.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VPY6"/>
    <dbReference type="EnsemblMetazoa" id="ACON006377-RB">
        <property type="protein sequence ID" value="ACON006377-PB"/>
        <property type="gene ID" value="ACON006377"/>
    </dbReference>
    <dbReference type="GeneID" id="120951521"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000983"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON006377"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034333"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON006377-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="144" mass="16153" checksum="AD4871B9D3BA4E13" modified="2020-08-12" version="1">MTMGKILCVLIASVNLVAMAYTVYSIMHYLDEQPLLEIFISPCLANMVLAVLLVIGIATQEISLIRLFKIFLYAQLVFALLMVVYGHRFFESVKLPMMVKAYFSIMFLVCAVELVIVSSTIENLRKRLNPAEGLVVYMPVGQPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VQ52</accession>
    <name>A0A6E8VQ52_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Malate dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LDH2/MDH2 oxidoreductase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VQ52"/>
    <dbReference type="EnsemblMetazoa" id="ACON006576-RC">
        <property type="protein sequence ID" value="ACON006576-PC"/>
        <property type="gene ID" value="ACON006576"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_013365"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON006576"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035282"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1530.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.60">
        <property type="entry name" value="Hypothetical oxidoreductase yiak, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043144">
        <property type="entry name" value="Mal/L-sulf/L-lact_DH-like_ah"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043143">
        <property type="entry name" value="Mal/L-sulf/L-lact_DH-like_NADP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036111">
        <property type="entry name" value="Mal/L-sulfo/L-lacto_DH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003767">
        <property type="entry name" value="Malate/L-lactate_DH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11091:SF0">
        <property type="entry name" value="MALATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11091">
        <property type="entry name" value="OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02615">
        <property type="entry name" value="Ldh_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89733">
        <property type="entry name" value="L-sulfolactate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON006576-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="364" mass="38486" checksum="FE7EC359C85F5648" modified="2020-08-12" version="1">MSTTGAAKKGLVAVEEARRFMVDCLIKSNTPPAHAKQQADLLVEADYRGHFSHGMNRLEMYINDLHKNACNGSAVPAVLNETPATAWVDGNNGLGAVVGNFCMDLAIRKAKEVGVGWVCAKRSNHYGIAGWYTLRAMNAGCIGMSMTNTSPLASPTRSKEAALGTNPISVGAPGKDGDGFVLDMATTAVAVGKIEMQRRKNEPIPVGWAQGPDGHPTTDASVAFDTACLMPLGGTELTSGYKGYGLGAMVEVFCGVLAGANYATKIRKWTHAGADSEADLGQCFVAINPACFAPGFEGRLSDLTGILRNMPMTDPNHPVLVAGDPELHHMAMVDKEGGLAYHVNQIKTCSELSERLGVKPIEVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VQ71</accession>
    <name>A0A6E8VQ71_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">PR domain zinc finger protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_040220443.2">
        <property type="nucleotide sequence ID" value="XM_040364509.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_049460962.1">
        <property type="nucleotide sequence ID" value="XM_049605005.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_049460963.1">
        <property type="nucleotide sequence ID" value="XM_049605006.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VQ71"/>
    <dbReference type="EnsemblMetazoa" id="ACON006592-RB">
        <property type="protein sequence ID" value="ACON006592-PB"/>
        <property type="gene ID" value="ACON006592"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON006592-RC">
        <property type="protein sequence ID" value="ACON006592-PC"/>
        <property type="gene ID" value="ACON006592"/>
    </dbReference>
    <dbReference type="GeneID" id="120948310"/>
    <dbReference type="CTD" id="38638"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_013589"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON006592"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035605"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001227">
        <property type="term" value="F:DNA-binding transcription repressor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008170">
        <property type="term" value="F:N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008276">
        <property type="term" value="F:protein methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19187">
        <property type="entry name" value="PR-SET_PRDM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.270.10:FF:000036">
        <property type="entry name" value="Blimp-1, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000748">
        <property type="entry name" value="PR domain zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000833">
        <property type="entry name" value="PR domain zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000132">
        <property type="entry name" value="PR domain zinc finger protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000211">
        <property type="entry name" value="PR domain zinc finger protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000436">
        <property type="entry name" value="PR domain zinc finger protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050636">
        <property type="entry name" value="C2H2-ZF_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044413">
        <property type="entry name" value="PRDM1_PR-SET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47772:SF13">
        <property type="entry name" value="GASTRULA ZINC FINGER PROTEIN XLCGF49.1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47772">
        <property type="entry name" value="ZINC FINGER PROTEIN 200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21549">
        <property type="entry name" value="PRDM2_PR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0391">Immunity</keyword>
    <keyword evidence="2" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SET" evidence="16">
        <location>
            <begin position="55"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="847"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="875"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="903"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="931"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="311"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="514"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1002"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1036"/>
            <end position="1141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="341"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="557"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="591"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="602"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="642"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="673"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="702"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="744"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="756"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="802"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="1043"/>
            <end position="1053"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1080"/>
            <end position="1089"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="14">
        <location>
            <begin position="1109"/>
            <end position="1122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON006592-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="19">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1148" mass="126946" checksum="547F9BD5F7F4223A" modified="2020-08-12" version="1">MSGANPPRSVTPNAQQDYDVTRMREEDFERLAVYIVPDMPCEKGVPGRAEKTLPRSLTLKPSLVLSTPTSTTEGVWSTGVIPRGTRFGPFEGVRTPSHPSDKQLWRYFWRVQELRHVSYGNIKIFKDSDFYYLDGSDTAQSNWMRYVASAYSFSVMNLVACQHQEHIYFYTIRDIMPNEELMVWYCRDFAKRLGYDIDPERATYSICREEVVKKTYKQPVPPEIAYNHVKHVMGLYLPAMVRSTPTPSPSPPAPATVGGHVPSAKHHALHQTVLREPRNSSSAAAVTGDESLRDRSPIRIVIREQSPLLRESVKEVDADSPISQTKETAYEHQLTPNDGSVRSDEGYHSHGYHEDGFTPPEDSSDSESEHNYVLDCSKKAIEPKETSVGQHKVSTSEPCDKNEYRKVKMKMPLKYEFKNNSKNFKVEPELEPTEREVEPAVPASSEPVVVGASMREVTPATSTVIVLDTTPESSVVVPLTKSYYEAEPTSTSPATFIRYTPPSSSSILETILTGSQRASPSADDPHRRQPNATPPPTSPTEMAYSYKKSQRYGTACSPDSSSQNPQAPEPAEPGHGPNRSQPHHHGAAGPEPKEADHADQHHLHHHLHHLHHHHNHHHHLQAHHHPHHHLQQPHQQPHQSGYHHERLHESREEPTAAASPSPSPIVYPGEHHPHLHHPHHHLPPHHHHRTHSPVAYESPVPSYTATSSSGSLYSTSNGSASVINSASSSTYSPPLNGGHYERLTTSSTTTTIRSVSPPPPPPTQPAPAPGYVQLQSKGQLPLGHPLMQPLTPLTHMSPGRISPPSSLSPDGGSYSRSGSPMSPGSPNSRGYRSLPYPLRKRDGKMHYECNVCSKTFGQLSNLKVHLRTHSGERPFKCNVCTKSFTQLAHLQKHHLVHTGEKPHQCDICKKRFSSTSNLKTHLRLHSGQKPYACDLCPQKFTQFVHLKLHKRLHTNDRPYVCQGCDKKYISASGLRTHWKTTSCKPNNLEEELAMAAAATSECLDKDGQPDGEGGREFYEMSHHGSPGAVVVQQTAAALSPASHHQHHQHAGHHHGTEQVIHKVSAGKIQQQQHQGPSPPNSSNNNSPNSTNHNLAPSPHLHYGGHLVQSGGGGNGTASGGPGPAQSQHEPANRPSVIESSQPMVIECT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VQ80</accession>
    <name>A0A6E8VQ80_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Musashi family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VQ80"/>
    <dbReference type="EnsemblMetazoa" id="ACON006656-RA">
        <property type="protein sequence ID" value="ACON006656-PA"/>
        <property type="gene ID" value="ACON006656"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005904"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON006656"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039896"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12323">
        <property type="entry name" value="RRM2_MSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000020">
        <property type="entry name" value="RNA-binding protein Musashi homolog 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034126">
        <property type="entry name" value="MSI_RRM2"/>
    </dbReference>
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        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48032">
        <property type="entry name" value="RNA-BINDING PROTEIN MUSASHI HOMOLOG RBP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48032:SF18">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="75"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON006656-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="237" mass="26277" checksum="D14F6D7ED7D81EE0" modified="2020-08-12" version="1">SLRDYFSKFGEITEVMVMKDPTTRRSRGFGFITFGDPASVDKVLAHGTHELDGKKIDPKVAFPRRAHPKMVTRTKKIFVGGLSAPTTLEDVKSYFEQFGPIEDAMLMFDKQTNRHRGFGFVTFQSEDVVDKVCEIHFHEINNKMVECKKAQPKEVMLPANLAKTRTAGRGTYDFMWSLGTLPDGFPAAAYAAYAAGRGFSGYPSFGLPYPTGVMGVMPENRIIMGDYLSMGPSTSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VRX0</accession>
    <name>A0A6E8VRX0_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ninjurin a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ninjurin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VRX0"/>
    <dbReference type="EnsemblMetazoa" id="ACON006745-RB">
        <property type="protein sequence ID" value="ACON006745-PB"/>
        <property type="gene ID" value="ACON006745"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000996"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON006745"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030938"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042246">
        <property type="term" value="P:tissue regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007007">
        <property type="entry name" value="Ninjurin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12316:SF20">
        <property type="entry name" value="NINJURIN-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12316">
        <property type="entry name" value="NINJURIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04923">
        <property type="entry name" value="Ninjurin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="185"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="230"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="41"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="60"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON006745-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="257" mass="27734" checksum="9E6B5AA8EDC7571A" modified="2020-08-12" version="1">MTSTGEKDTSAVEINIEESAGSDMPDAGRTTANPAHTLAARPNGRNRSRSNSEPPMAGPELDDDDDELDDGVDLDDPKSNRGIDSGFLDPVDNGSVDTGVSRAGRRGGRRNQAPAGPTYRPGFAPTFDADGTRVVQTTATGQVIPDVNVYQQKKNLAQGMMDLALLSANANQLRYVLESYSRHPYFYVNLVFISTSIIAQVAVGIGLIWKSRYDIKKENDFCKADRINNLITIGIFIITLVNVLISAFGVAETQPVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VRX5</accession>
    <name>A0A6E8VRX5_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Ceramide transfer protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_040221798.1">
        <property type="nucleotide sequence ID" value="XM_040365864.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VRX5"/>
    <dbReference type="EnsemblMetazoa" id="ACON007093-RB">
        <property type="protein sequence ID" value="ACON007093-PB"/>
        <property type="gene ID" value="ACON007093"/>
    </dbReference>
    <dbReference type="GeneID" id="120948965"/>
    <dbReference type="CTD" id="38928"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_008773"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007093"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042099"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035621">
        <property type="term" value="P:ER to Golgi ceramide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08872">
        <property type="entry name" value="START_STARD11-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.530.20:FF:000003">
        <property type="entry name" value="Collagen type IV alpha-3-binding protein-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041952">
        <property type="entry name" value="STARD11_START"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002913">
        <property type="entry name" value="START_lipid-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051213">
        <property type="entry name" value="START_lipid_transfer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19308:SF53">
        <property type="entry name" value="CERAMIDE TRANSFER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19308">
        <property type="entry name" value="PHOSPHATIDYLCHOLINE TRANSFER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01852">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00234">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50848">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="START" evidence="6">
        <location>
            <begin position="117"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007093-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="330" mass="37758" checksum="B533F86AD136C003" modified="2023-06-28" version="2">MPSLTTARLFKGLLRKEIEGPHSTIPEDEFFDAVETGLDKIEEDRQLRVRLKYQSQQSQISNVSSLHSPSAETEDEIPQLAEDFGTGAQAKSHKLWPEIDRICTEQLTQARQGVGEDGNGWQLFADEGEMKMYRREEEVDGMVIDPLKSCHVVKGVTAREMCHYFFDPAYRNDWETTLEDVQIVDNVAPDTLVFLQTYKRIWPASQRDALFWSHMRRITDNLDAGANDVWIVCNHSNQNEEYPPANQGKCVRIYLTVILVCQTYLPPGKDSKTATRDDLTCKITYCSTVNPGGWAPATVLRAIYKKEYPKFLKRFTGYVVDQCKAKPIMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VRY3</accession>
    <name>A0A6E8VRY3_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cleavage and polyadenylation specificity factor subunit 5</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VRY3"/>
    <dbReference type="EnsemblMetazoa" id="ACON007242-RB">
        <property type="protein sequence ID" value="ACON007242-PB"/>
        <property type="gene ID" value="ACON007242"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005607"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007242"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031725"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005849">
        <property type="term" value="C:mRNA cleavage factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016706">
        <property type="entry name" value="Cleav_polyA_spec_factor_su5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13047">
        <property type="entry name" value="PRE-MRNA CLEAVAGE FACTOR IM, 25KD SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13869">
        <property type="entry name" value="NUDIX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007242-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="141" mass="15975" checksum="00FB044603D59C16" modified="2020-08-12" version="1">MTSQAVNKTGSGWPRRNSHGTGLTEMNKLQTNQSMTLNRTINLYPLTNYTFGTKEPLFEKDPSVPARFQRMRDEFDKIGMRRSVEGVLLVHEHGLPHVLLLQLGTTFFKLPGGELSAGEDEVDGLKRLLTEHYLPYLRTTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VRZ2</accession>
    <name>A0A6E8VRZ2_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Flightin</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VRZ2"/>
    <dbReference type="EnsemblMetazoa" id="ACON007249-RA">
        <property type="protein sequence ID" value="ACON007249-PA"/>
        <property type="gene ID" value="ACON007249"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_013377"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007249"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038554"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007249-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="136" mass="16398" checksum="26D24446D4707EE3" modified="2020-08-12" version="1">MDDGAASTGVIVKNKPGAKPLNIVDDFNRRKMPLYKHWVRPQFLQYNYMYDYRVNYYDDVIDYLDRRSRGVASEIPRPQTWAERVLRTQKTATRDINDAYNYTSISHKKDDKKLMYTLRKSRPLVRLYMSSTYRRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VTI6</accession>
    <name>A0A6E8VTI6_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Endothelin-converting enzyme 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">Ngousso</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M13 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_049464578.1">
        <property type="nucleotide sequence ID" value="XM_049608621.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VTI6"/>
    <dbReference type="EnsemblMetazoa" id="ACON007796-RB">
        <property type="protein sequence ID" value="ACON007796-PB"/>
        <property type="gene ID" value="ACON007796"/>
    </dbReference>
    <dbReference type="GeneID" id="120957236"/>
    <dbReference type="CTD" id="33005"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_014431"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007796"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040201"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08662">
        <property type="entry name" value="M13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1380.10">
        <property type="entry name" value="Neutral endopeptidase , domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000718">
        <property type="entry name" value="Peptidase_M13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018497">
        <property type="entry name" value="Peptidase_M13_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042089">
        <property type="entry name" value="Peptidase_M13_dom_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008753">
        <property type="entry name" value="Peptidase_M13_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11733:SF167">
        <property type="entry name" value="FI17812P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11733">
        <property type="entry name" value="ZINC METALLOPROTEASE FAMILY M13 NEPRILYSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01431">
        <property type="entry name" value="Peptidase_M13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05649">
        <property type="entry name" value="Peptidase_M13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00786">
        <property type="entry name" value="NEPRILYSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51885">
        <property type="entry name" value="NEPRILYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M13 N-terminal" evidence="11">
        <location>
            <begin position="121"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M13 C-terminal" evidence="10">
        <location>
            <begin position="578"/>
            <end position="782"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF05649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007796-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="785" mass="90004" checksum="45A1255B99F9C3F9" modified="2020-08-12" version="1">MTRYKQAQFADEDSSSIGSIQINETTRSPTMHIRYHAARGSSLWNARSKLEKVLLSLLALSIILIVVLSSLLASDTTRILHVRPHVGSDPFGVDEQLPCLNQHCIFAASEILHSIDWSVDPCDDFYAFSCNQWIRNNPIPEGKSMWGLFGKLEQQNQLVLKNALERPLAEFKSKAEKKAKLYYQSCLDEDETMEKLGAEPLQKLLRQIGGWNVTANASGFDVQRWSLQRTLQTLQIRYNMGGLFGWAVGEDDRNSSKHIIQIDQGGLTLPSRDNYLNKTANAKILTAYLEYMTKVSVLLGADETDARRQMLEVIEFETRLANITTPQDMRRDEETLYHPMTLAMLQEKAPFINWQEHFEEAFRLVRRKITEKERVVVYAPEYLEKLNILMKEYTSTDEKKIILNNYLVWQTVRTLTACLSKAFRDAYKGLRKALMGSDGGEELWRYCVSDTSNVLGFAVGAMFVRDVFHGDSKPQAEEMINQVRDAFKENFKNLGWMDAETRRLAVEKADAISDMIGFPDYILYPEELDRKYQDLNIDPKTYFDNNINYNIYSLKKNLEKLDQPVNKTKWGMTPPTVNAYYTPTKNQIVFPAGILQNPFFDIKNSKSLNYGAMGVVMGHELTHAFDDQGREYDKYGNLHQWWNNQTIERFKNQTECFNQQYSAYRINGKTINGKQTMGENIADNGGLKAAFHAYINNEKNSYTDTDTLPLPGVNMTHRQLFFVSFAQVWCSAVTDETTTLQIEKDSHSPPKYRVIGPLSNLKEFSDTFHCPLGTGMNPIDKCVVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VWA0</accession>
    <name>A0A6E8VWA0_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the DEAD box helicase family. DEAH subfamily. PRP16 sub-subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8VWA0"/>
    <dbReference type="EnsemblMetazoa" id="ACON008528-RA">
        <property type="protein sequence ID" value="ACON008528-PA"/>
        <property type="gene ID" value="ACON008528"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_013126"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008528"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035012"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034458">
        <property type="term" value="F:3'-5' RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003006">
        <property type="term" value="P:developmental process involved in reproduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1080:FF:000001">
        <property type="entry name" value="Pre-mRNA-splicing factor ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000007">
        <property type="entry name" value="Pre-mRNA-splicing factor ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000313">
        <property type="entry name" value="Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF91">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR ATP-DEPENDENT RNA HELICASE PRP16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="11">
        <location>
            <begin position="286"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="12">
        <location>
            <begin position="474"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="229"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="243"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008528-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="962" mass="109815" checksum="46FCD8A35FCC853C" modified="2020-08-12" version="1">TFSRYEPSTPRSRHRSKQPSSSNWTDDEDEDGEPSGPRSSWDFPTPKPFSGAEFTEADREQWNEEQLRLDREWYGSDDERQMNEFSELNSQYLQQREQQQQSRNNRRISAQQRQINKDNELWEKNRLLTSGVVMSVNVNEDFDEEALERVHLLVHHTVPPFLDGRIVFTKQPEPVVPVREPTSDMAINARKGSALVRTYRELKERKRAQAKHWQLGGTKLGNIMGVAKDKDEQDPADGDDEAYDSRKDQKYADHIGGEKGEFAPRRKTIQQQRRSLPVFAVRQDLLNIIRENSIIIIVGETGSGKTTQLTQYLHEDGYSRHGMIGCTQPRRVAAMSVAKRVSDEMDCSLGQEVGYAIRFEDCTSEKTVIKYMTDGILLRESLRDKELDGYSVIIMDEAHERSLSTDVLFGLLREIVAKRRDLKLIVTSATMDAGKFSNFFGNVPTFTIPGRTFPVDVFYGKNVCEDYVDGAVKQVLQIHLQPTEGDILVFMPGQEDIEVTCEVLAERLGEIDNAPELSILPIYSQLPSDLQAKIFHRSADGTRKCVVATNIAETSLTVDGITYVIDSGYCKLKVYNPRIGMDALQIYPISQANANQRSGRAGRTGPGQAFRLYTERQYKDELLHLTVPEIQRTNLANTVLLLKSLGVSDLLQFHFMDPPPQDNILNSLYQLWILGALDHTGALTPLGRQMAEFPLDPPQCQMLIVANEMGCSEEILIIVSMLSVPSIFYRPKGREEEADSVREKFQVPESDHLTYLNVYQQWKMNKYSGSWCNEHFIHVKAMRKVREVRQQLKDIYSQQQRLTLKSCGTDWDVVRKCICSAYFYQAARLKGIGEYVNLRTGMPCHLHPTSALYGLGTTPDYVVYHELIMTAKEYMQCATAVDGYWLAELGPMFFSVKETGKSGRDKRRQAVEHQNAMEVQMREAQQQMEQRKQDEEKHKFTIKQEIVTPGATPRRTPARIGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VWA1</accession>
    <name>A0A6E8VWA1_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="3">N-myc downstream regulated</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NDRG family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VWA1"/>
    <dbReference type="EnsemblMetazoa" id="ACON008821-RB">
        <property type="protein sequence ID" value="ACON008821-PB"/>
        <property type="gene ID" value="ACON008821"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON008821-RC">
        <property type="protein sequence ID" value="ACON008821-PC"/>
        <property type="gene ID" value="ACON008821"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011297"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008821"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041407"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="entry name" value="protein NDRG3 isoform X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004142">
        <property type="entry name" value="NDRG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11034">
        <property type="entry name" value="N-MYC DOWNSTREAM REGULATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03096">
        <property type="entry name" value="Ndr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="18"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008821-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="433" mass="47030" checksum="BBC1ABD0DE436DB3" modified="2020-08-12" version="1">MPQSDGGYVSLGGGGPNSIYNSTTEPQSGSKSVMETVKRVIGTAIGAQDKHALLERDGTGAQPYIVSARDRDKTGKSANARMPSAPAHTAEEARLLGAMPVDPMDDIELRSVQLQYPNQRGSIVGACDLRRVPTDKGDILVAVQGDTTKPAIVTYHDLGLNYASSFAGFFNFPTMRSLLDNFCVYHVNAPGQEDGAPTFPEDYVYPTFDELASQMLFVMTHFNLKSIIGFGVGAGANILARFALANPDKVGALCLINCSSTAAGWIEWGYQLLNTRNLRTKGMTQGVLDYLMWHHFGRNPEERNLDLVQLYKSNFERSINPVNLAMLIDAYIKRTDLNIARTPSGSPQTSAPSLKMPVLNITGALSPHIDDTVTFNGRLIPEKTNWMKISDCGLVLEEQPGKLAEAFRLFLQGEGYVSKKLPSPTKAQAPAGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VWA4</accession>
    <name>A0A6E8VWA4_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Insulin receptor substrate 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Protein chico</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit. May mediate the control of various cellular processes by insulin-like peptides. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains. Involved in control of cell proliferation, cell size, and body and organ growth throughout development. Also has a role in a signaling pathway controlling the physiological response required to endure periods of low nutrient conditions. Insulin/insulin-like growth factor (IGF) signaling pathway has a role in regulating aging and is necessary in the ovary for vitellogenic maturation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Bindings to phosphatidylinositol 3-kinase and SHP2.</text>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON008859-RA">
        <property type="protein sequence ID" value="ACON008859-PA"/>
        <property type="gene ID" value="ACON008859"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_003426"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008859"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038234"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005158">
        <property type="term" value="F:insulin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043548">
        <property type="term" value="F:phosphatidylinositol 3-kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008286">
        <property type="term" value="P:insulin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048477">
        <property type="term" value="P:oogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01257">
        <property type="entry name" value="PH_IRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01204">
        <property type="entry name" value="PTB_IRS"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
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        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="PH_domain"/>
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        <property type="entry name" value="INSULIN RECEPTOR SUBSTRATE"/>
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    </dbReference>
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        <property type="entry name" value="INSULIN RECEPTOR SUBSTRATE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02174">
        <property type="entry name" value="IRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00628">
        <property type="entry name" value="INSULINRSI"/>
    </dbReference>
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        <property type="entry name" value="IRS"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="6" id="KW-0221">Differentiation</keyword>
    <keyword evidence="4" id="KW-0341">Growth regulation</keyword>
    <keyword evidence="7" id="KW-0896">Oogenesis</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
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        <location>
            <begin position="208"/>
            <end position="319"/>
        </location>
    </feature>
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        </location>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="PROSITE" id="PS50003"/>
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        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
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    <sequence length="2063" mass="213567" checksum="4A31268653D3D3DA" modified="2020-08-12" version="1">MSTLTNNANNSSSNSNSNNNNNSSSNNTNNNNNSIASYYQQYIQHYQLSPQGGTAPPSAGGSPTVTSINNNNNGPTSDNIHTINNNNISTSSSSTSSNQSQPTTAANSGSGGGGGVVGGILGVREVSAKLFLPFARAKMMHSSSSAASTPAKDQHQPGTPLDGTSMAGMNATPTGTPSATPGTTTGGSSAGMSGAGRASSLIIPKALNVVLHGYHRKHKTNKKKYFVVYGDVPGKAARLEYFDSEKKFKQSFQKGSAGCDGGTQPKRTIVLRTCFNINKRHDLKKQIISLYTKDDSFCIVFESEEELNLWLRTLLRLQRGEDSEGDPPRPTFEHVWEVYVQRKGLGDSYGILGNYRLCITDKTLSLVRIGSELTPHQEKRAENVEFLLASVRRCGHSQRYFYLEVGRSSKTGAGEIWMEVQDSIIAQNMYTTIMKSAKSKDDNLGPMIRIRSSSANAASKPTSMLFRRQTHTGQKPINCSPSSVSSGLSAVANMSSPAAMGNSTTAAMMMLPPSPSASMSKFHHYASTRQGGKEVPGSGGGITLSNLLATITHSLVGVGGGGAGSGAANGDSGPLATASGGSGGSGGSSSGGSFSNFSTSSRSSSRCSSTVSIQTVATVPLMGPPPPPPPPPPLEQPALCGVRRRHTRSLSHHSANAIYLTGAKPLRRLRSTLRRHSVSGVAIARERCDSLPSRNRTTSETMPHQQQQQQQHLLNQQQQQQHHHQLMLNSGSMPPPRMIPSSNRPHSVHISHSHSPPVNSSPLSPPPPATYSTGSDGSSLSIDESTDSFITGSLTPDEGQGYPKVINQLNSGCSIPEENSEEFIKDYGHIDLSAAGCGISQHDDFAVPAARRGGSPAPPSTVGSVEGSAYMEMCSPSGSSPGDPSGSCYMAMSPVADFSRGLCSSSSAHSRASSLAEDIVDGYVPMSMPRTAAAAPAAPPTEEYMLMDPTLNGGGTSSRLSNATAASNNAGSSIIGGGGMSSAASSCSITSGTPSADLRLSLEKVPAYIGQSDEHEVNDRPFRTYSVGSRPELIKRKLRVEMLSSGESSVGGSSAGGSASSRQRAFSVGSRAKVPRCSDVYKGYSAAPVIVSFASTSSQGSMLESSNNNSSSSTSSISHAGSYGASIGDTLPMDELAGGGSAGGSSMGRKSGSGSLLPDGAGGGKPPSHGGSHGRMSDLMEIDYSSPRQQPARPSTRSSEPSGMEDYLDMSGNNNEEEQRARGQGATSSGYVEMRPGRLNSQSEENDYLNMTPGGGRDEPDCCPAAASIATSSPIQIQHHAKQPADGAGSAHSSSSNNNNNNNYLEMYPRAAVAQKEQKRTSSATSTTTTATKVPSEDYLNMAPLMISSLGEASGSEEEKMHGPDEEPLLESSSAPPDGYMEMSWNHHGPVTATGPVSSSASSSATSSARTVPTNGGDDYMNMSCGSGSQSEDASRTYSAPITIQPGAGKCRLDLSGESKVEAGANIPSYLPLGQHPDARKSVHHPHHQSAMIVALQQHLPLAAQAPIQTTTGQQSGSIGFSPSKPAETTPERARCDSRDSGIVTPSGSQPTIFPFSPASPTKPFAPAQDEPMGGESLDDLSQNYAELSLGRQQQQQLQQAQTEPSPAIRKLSTGSNRGSGSFSKLQLVASQPDYVNYCPATPPISGDVAPPASNMSLNVLPSAQSRTRSSIDDDGAGDYALMNPASSRKLSTTSMGSMGAASLSPAGVVPPGSSAVGSITPPVLVARKSTLLLSSPQMGFKPIASSQDAELLLQTKAVPHATANSPKALNLGRRTGEPAFSFTEQQQQQMAPLQRSISTGTRPNSVNSELIGRPIGTPAGPSTPGAASHSSATAAGSTRPSSANSERLPVISATSSSASSTSTLCESKNQSPSASSTALAMVVGSSTTTSPPSVPGSRPDSVSSITNPHIVSRPPSVSSERELHYASLDLPPCSSSGKQTVAASPFAPGTMMLLAAAAAATTPAATSSAAAPVTKMDVDEPLPGSNEARTSIDSSSPTTAAGLQQQQQPVRCAAFTYAQIDFVRSQAQSQQGQTGTGPAGASGPTGSTTTTTTSTPPQSSQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VWD4</accession>
    <name>A0A6E8VWD4_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Insulin receptor substrate 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Protein chico</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit. May mediate the control of various cellular processes by insulin-like peptides. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains. Involved in control of cell proliferation, cell size, and body and organ growth throughout development. Also has a role in a signaling pathway controlling the physiological response required to endure periods of low nutrient conditions. Insulin/insulin-like growth factor (IGF) signaling pathway has a role in regulating aging and is necessary in the ovary for vitellogenic maturation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Bindings to phosphatidylinositol 3-kinase and SHP2.</text>
    </comment>
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        <property type="protein sequence ID" value="ACON008859-PC"/>
        <property type="gene ID" value="ACON008859"/>
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        <property type="component" value="Unplaced"/>
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        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="term" value="F:phosphatidylinositol 3-kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048477">
        <property type="term" value="P:oogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="PH_IRS"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    <keyword evidence="4" id="KW-0341">Growth regulation</keyword>
    <keyword evidence="7" id="KW-0896">Oogenesis</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00046145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008859-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="2204" mass="227381" checksum="161B5DF3D246F5C0" modified="2020-08-12" version="1">MSTLTNNANNSSSNSNSNNNNNSSSNNTNNNNNSIASYYQQYIQHYQLSPQGGTAPPSAGGSPTVTSINNNNNGPTSDNIHTINNNNISTSSSSTSSNQSQPTTAANSGSGGGGGVVGGILGVREVSAKLFLPFARAKMMHSSSSAASTPAKDQHQPGTPLDGTSMAGMNATPTGTPSATPGTTTGGSSAGMSGAGRASSLIIPKALNVVLHGYHRKHKTNKKKYFVVYGDVPGKAARLEYFDSEKKFKQSFQKGSAGCDGGTQPKRTIVLRTCFNINKRHDLKKQIISLYTKDDSFCIVFESEEELNLWLRTLLRLQRGEDSEGDPPRPTFEHVWEVYVQRKGLGDSYGILGNYRLCITDKTLSLVRIGSELTPHQEKRAENVEFLLASVRRCGHSQRYFYLEVGRSSKTGAGEIWMEVQDSIIAQNMYTTIMKSAKSKDDNLGPMIRIRSSSANAASKPTSMLFRRQTHTGQKPINCSPSSEDQSNNGQNSESSSSATSVNTVVLVGSPPKAGTSAGLPYSAGAPGAKPRPPVVANPSLPACAENSSSLNTSTTSTITTAACTSTTAAAAAATACSSSFVGPFQSSSDSSYHAPMIVPFAGITPAVSTGNVVASTPAAMFSSNSSLASIGSCSPSDAYVPLVPPGHASTSQASGGGGGGVPTRGGLVGSPRLGVTAQSPNYRRTTSVKKSAGSDAGGGSTTSPLQATRSHQPSRHYHQQQLPSTSSHHSHHPTHLPHPPPPHHPAPYPHHGTQAVATGAVVGPHPATPSVVSSSGCCSIGGSSSSIVSNRSSSNYSSNSSSTSTIAVTNEFSSSSTDHGVAIARERCDSLPSRNRTTSETMPHQQQQQQQHLLNQQQQQQHHHQLMLNSGSMPPPRMIPSSNRPHSVHISHSHSPPVNSSPLSPPPPATYSTGSDGSSLSIDESTDSFITGSLTPDEGQGYPKVINQLNSGCSIPEENSEEFIKDYGHIDLSAAGCGISQHDDFAVPAARRGGSPAPPSTVGSVEGSAYMEMCSPSGSSPGDPSGSCYMAMSPVADFSRGLCSSSSAHSRASSLAEDIVDGYVPMSMPRTAAAAPAAPPTEEYMLMDPTLNGGGTSSRLSNATAASNNAGSSIIGGGGMSSAASSCSITSGTPSADLRLSLEKVPAYIGQSDEHEVNDRPFRTYSVGSRPELIKRKLRVEMLSSGESSVGGSSAGGSASSRQRAFSVGSRAKVPRCSDVYKGYSAAPVIVSFASTSSQGSMLESSNNNSSSSTSSISHAGSYGASIGDTLPMDELAGGGSAGGSSMGRKSGSGSLLPDGAGGGKPPSHGGSHGRMSDLMEIDYSSPRQQPARPSTRSSEPSGMEDYLDMSGNNNEEEQRARGQGATSSGYVEMRPGRLNSQSEENDYLNMTPGGGRDEPDCCPAAASIATSSPIQIQHHAKQPADGAGSAHSSSSNNNNNNNYLEMYPRAAVAQKEQKRTSSATSTTTTATKVPSEDYLNMAPLMISSLGEASGSEEEKMHGPDEEPLLESSSAPPDGYMEMSWNHHGPVTATGPVSSSASSSATSSARTVPTNGGDDYMNMSCGSGSQSEDASRTYSAPITIQPGAGKCRLDLSGESKVEAGANIPSYLPLGQHPDARKSVHHPHHQSAMIVALQQHLPLAAQAPIQTTTGQQSGSIGFSPSKPAETTPERARCDSRDSGIVTPSGSQPTIFPFSPASPTKPFAPAQDEPMGGESLDDLSQNYAELSLGRQQQQQLQQAQTEPSPAIRKLSTGSNRGSGSFSKLQLVASQPDYVNYCPATPPISGDVAPPASNMSLNVLPSAQSRTRSSIDDDGAGDYALMNPASSRKLSTTSMGSMGAASLSPAGVVPPGSSAVGSITPPVLVARKSTLLLSSPQMGFKPIASSQDAELLLQTKAVPHATANSPKALNLGRRTGEPAFSFTEQQQQQMAPLQRSISTGTRPNSVNSELIGRPIGTPAGPSTPGAASHSSATAAGSTRPSSANSERLPVISATSSSASSTSTLCESKNQSPSASSTALAMVVGSSTTTSPPSVPGSRPDSVSSITNPHIVSRPPSVSSERELHYASLDLPPCSSSGKQTVAASPFAPGTMMLLAAAAAATTPAATSSAAAPVTKMDVDEPLPGSNEARTSIDSSSPTTAAGLQQQQQPVRCAAFTYAQIDFVRSQAQSQQGQTGTGPAGASGPTGSTTTTTTSTPPQSSQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VWF8</accession>
    <name>A0A6E8VWF8_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VWF8"/>
    <dbReference type="EnsemblMetazoa" id="ACON008310-RC">
        <property type="protein sequence ID" value="ACON008310-PC"/>
        <property type="gene ID" value="ACON008310"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005253"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008310"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031613"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042734">
        <property type="term" value="C:presynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070073">
        <property type="term" value="P:clustering of voltage-gated calcium channels"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007274">
        <property type="term" value="P:neuromuscular synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007270">
        <property type="term" value="P:neuron-neuron synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032751">
        <property type="entry name" value="Fuseless"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35270">
        <property type="entry name" value="FUSELESS, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35270:SF2">
        <property type="entry name" value="FUSELESS, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15993">
        <property type="entry name" value="Fuseless"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="17"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="58"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="88"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="131"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="203"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="215"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="226"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008310-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="272" mass="31051" checksum="2A521F718C82CDBD" modified="2020-08-12" version="1">MFSFPTRFRMKGSKDPGLYVLDCMFSVLVIGSLVVFVWRGLWVLLDLKLFPEDHGTSAWASIIIGYGVTGVTFSLQPLMRWTCDRLTGFWRVVVADLFLFFSFIGTINVWRGIWQALDTYFLPDDPLLSNWITHGAALLLLILLNCSNSVLVRGVYIDAEEPAGQCVVFPVYYIRLFFQKERKKKQRRLFDALERADLNSTGGVLQDKKHHHSLQTAAQQPAQQKEQQKEAHQHHIAADQNHQVIRNLEAVPLTIIDDPKSNGDHSSQPTRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VYE8</accession>
    <name>A0A6E8VYE8_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="8">C2H2-type domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_040231581.2">
        <property type="nucleotide sequence ID" value="XM_040375647.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040231582.2">
        <property type="nucleotide sequence ID" value="XM_040375648.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VYE8"/>
    <dbReference type="EnsemblMetazoa" id="ACON009662-RA">
        <property type="protein sequence ID" value="ACON009662-PA"/>
        <property type="gene ID" value="ACON009662"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON009662-RC">
        <property type="protein sequence ID" value="ACON009662-PC"/>
        <property type="gene ID" value="ACON009662"/>
    </dbReference>
    <dbReference type="GeneID" id="120955120"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002315"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON009662"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037361"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001399">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000072">
        <property type="entry name" value="zinc finger protein 143 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000340">
        <property type="entry name" value="zinc finger protein 473 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226">
        <property type="entry name" value="ZINC FINGER AND SCAN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226:SF371">
        <property type="entry name" value="ZINC FINGER PROTEIN 112-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13894">
        <property type="entry name" value="zf-C2H2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="168"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="232"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="260"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="288"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="316"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="344"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="374"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="402"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="14"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="75"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="128"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="450"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="611"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="719"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="14"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="75"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="86"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="140"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="475"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="491"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="535"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="569"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="615"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="637"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="667"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="727"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="795"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="817"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="889"/>
            <end position="898"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009662-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="898" mass="96324" checksum="33DE319DA64E2BBB" modified="2020-08-12" version="1">MVYYSGLPLLMTQQQQQQQQQQQQQQHGPDGTILQLSYNDANSTEQYVVAGQPSRLGVSHPYQQQQPQHHTISLTNGLTISNGPQSCSSSSSSSSGSSSTNGQLTIVLNPPAAGQLVSLGGSALPGTYTGPISPAGDRGASSSPEQSTYHPVQSGGGGASATPVGSKFRCDQCNISFGSKSAHTSHMKSHAKQQQASEKLTAAAKLGETTITASGGSGGGTGSPAGPQPDPYQCDVCKKTFAVPARLVRHYRTHTGERPFECEFCHKMFSVKENLQVHRRIHTKERPYKCEICGRAFEHSGKLHRHMRIHTGERPHKCNVCGKTFIQSGQLVIHMRTHTGEKPYKCPVEGCGKGFTCSKQLKVHSRTHTGEKPYHCDICFRDFGYNHVLKLHRVQHYGAKCYKCTICDETFKSKKEMEAHIKGHANELPDDEEAAAAAAASDAGGACKLEDVSTSGTEGSIKLSSSALEGGGSGSNSNSSSSFDYSSSMSHTEGTEQSGGPSSDEPSGHFAIRKSGSSSPSFAVGAKEAGQQVGADEEEEEDEEENDEEEDETTDDGDRKQMPRYESMGPSSFDQSLQRAYGGGVAPALLAAASIAAAVENGCTEVKYCPELKQQRSISPVQPSALSITRNTPPPSSGSSSSSSSSSTSSVLSMTPSGGTSSTLLEPAGSSAPSGPSAGQFVYEPMAIMKHHGYFAPASQITEFRSSSDIVRQVEAAIAGTENLLTPPRSSPESPDRSSSPESDSVLMADRDNNTLPPRKRKLYFKDTQHTVSASPKMASLEVTAPQHYGVSVVQQQQPTPQHHQQQQPPLDAHSGHQQLFHPHQQQQQVRQLHYQQQQRPEDERSMKLEVLQHVSACQRQPSPTLQAPAAPPQQPQQTSAPSVIRMSSVIQYANKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VYE9</accession>
    <name>A0A6E8VYE9_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">threonine--tRNA ligase</fullName>
            <ecNumber evidence="3">6.1.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Threonyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>tRNA(Thr) + L-threonine + ATP = L-threonyl-tRNA(Thr) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24624"/>
            <dbReference type="Rhea" id="RHEA-COMP:9670"/>
            <dbReference type="Rhea" id="RHEA-COMP:9704"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78534"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.3" evidence="3"/>
    <dbReference type="RefSeq" id="XP_040230833.2">
        <property type="nucleotide sequence ID" value="XM_040374899.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VYE9"/>
    <dbReference type="EnsemblMetazoa" id="ACON008929-RA">
        <property type="protein sequence ID" value="ACON008929-PA"/>
        <property type="gene ID" value="ACON008929"/>
    </dbReference>
    <dbReference type="GeneID" id="120954728"/>
    <dbReference type="CTD" id="45784"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001183"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008929"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031429"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004829">
        <property type="term" value="F:threonine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006435">
        <property type="term" value="P:threonyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01667">
        <property type="entry name" value="TGS_ThrRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00860">
        <property type="entry name" value="ThrRS_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00771">
        <property type="entry name" value="ThrRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000009">
        <property type="entry name" value="Threonine--tRNA ligase 2, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.800:FF:000003">
        <property type="entry name" value="Threonine--tRNA ligase 2, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000006">
        <property type="entry name" value="Threonine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.980.10:FF:000003">
        <property type="entry name" value="Threonine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.980.10">
        <property type="entry name" value="Threonyl-trna Synthetase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00184">
        <property type="entry name" value="Thr_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002320">
        <property type="entry name" value="Thr-tRNA-ligase_IIa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047246">
        <property type="entry name" value="ThrRS_anticodon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033728">
        <property type="entry name" value="ThrRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012947">
        <property type="entry name" value="tRNA_SAD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00418">
        <property type="entry name" value="thrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11451:SF46">
        <property type="entry name" value="THREONINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11451">
        <property type="entry name" value="THREONINE-TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02824">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07973">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01047">
        <property type="entry name" value="TRNASYNTHTHR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00863">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="TGS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="ThrRS/AlaRS common domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TGS" evidence="14">
        <location>
            <begin position="82"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="13">
        <location>
            <begin position="350"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="30"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008929-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="724" mass="82961" checksum="9AE975743176739A" modified="2020-08-12" version="1">MPENAPTAELAGLDLNKQKASKAKKEKQKNAAADKAAAPAPASGQASGAAQEMKDGPEFIDHRIKIYDELMAQYKEELEKREKTPIKVTLPDGKQVDGLSWQTTPYDVARGISQGLADNTVIARVNNELWDLDRPLEGDCKLQLLKFDDPDAQAVFWHSSAHILGEAMEKRYGGHLCYGPPIDNGFYYDMFLEGSGISNQDYGALESEVKRIVKEKQPFERLEMKKADLLRMFEYNQFKVRILNEKVTTETTTVYRCGPLIDLCRGPHVRNTGKVKALKVVKNSATYWEGKADAETLQRVYGISFPDPKQLKEWEKIQEEAAKRDHRKLGKEQELFFFHELSPGSCFFQPKGAHIYNTLMGFIRSEYRKRGFQEVISPNIYNAKLWQTSGHWQHYAENMFSFESEKETFALKPMNCPGHCLMFDHRNRSWRELPLRMADFGVLHRNELSGALTGLTRVRRFQQDDAHIFCMPDQIREEITGCLDFLRHVYTIFGFTFNLVLSTRPEKYLGEIEVWNEAEKALADSLDKFGEPWKENPGDGAFYGPKIDITIMDALKRNHQCATIQLDFQLPIRFNLNYIDDSGEKKRPVIIHRAVLGSVERMIAILTENYAGKWPFWLSPRQVMVVPVGPAYDDYADQVRQRLHEAGFMVEADLDAGDTMNKKIRNAQLAQFNFILVVGEKERSSETVNVRTRDNKVHGEVTVNDLVTKLSKLRDEYARGEDQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VYF3</accession>
    <name>A0A6E8VYF3_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Alpha-taxilin</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the taxilin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040239136.2">
        <property type="nucleotide sequence ID" value="XM_040383202.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VYF3"/>
    <dbReference type="EnsemblMetazoa" id="ACON009667-RA">
        <property type="protein sequence ID" value="ACON009667-PA"/>
        <property type="gene ID" value="ACON009667"/>
    </dbReference>
    <dbReference type="GeneID" id="120959638"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002410"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON009667"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030638"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026183">
        <property type="entry name" value="Taxilin_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16127:SF13">
        <property type="entry name" value="GH01188P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16127">
        <property type="entry name" value="TAXILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09728">
        <property type="entry name" value="Taxilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="355"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="69"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="267"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="15"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="379"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="403"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009667-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="425" mass="49155" checksum="78A4A79FA42A4138" modified="2020-08-12" version="1">MDVQAGNGEPTRSNAEAKKLLREDKQREAKIEEQLAKALSGMTVEEKYATVHKRLVESEKENRRLVALHKQYDRTLEASKREKENLILEHDKMAMTKTKLEGLCRELQRQNKTIKDESLAQIQQEEEKRKETQEKFQQSLNDIQAVMNENNDKNMKLKEDNMEMAKKFKFILEQYELRDQQMDKMNKQMELVTQLNDAKLAKLQMQANSEKEQFLAEKLVLMTELQKVKKQLADLQSVESHLRGQVNMYSDKYGEFQDSLKKSRSIYEGYQEDMKKMSKKMKTLEKETMAWKSRWETSNATVQKMLDEQIEREKHLTKTTRQLTQLQKLCRTLQAERATYLAALKEANLPVPAEPLEEEEKLAESAPSPPSETRKQKVKQTNANKQVNGHATEKPNPSTPAGSVSSPASQEQESAVQQEVSAESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W021</accession>
    <name>A0A6E8W021_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">V-type proton ATPase subunit H</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment. Subunit H is essential for V-ATPase activity, but not for the assembly of the complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">V-ATPase is a heteromultimeric enzyme made up of two complexes: the ATP-hydrolytic V1 complex and the proton translocation V0 complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the V-ATPase H subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040231438.1">
        <property type="nucleotide sequence ID" value="XM_040375504.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8W021"/>
    <dbReference type="EnsemblMetazoa" id="ACON009486-RA">
        <property type="protein sequence ID" value="ACON009486-PA"/>
        <property type="gene ID" value="ACON009486"/>
    </dbReference>
    <dbReference type="GeneID" id="120955040"/>
    <dbReference type="CTD" id="34997"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009992"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON009486"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034926"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000221">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase, V1 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00256">
        <property type="entry name" value="VATPase_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000067">
        <property type="entry name" value="V-type proton ATPase subunit H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.150:FF:000001">
        <property type="entry name" value="V-type proton ATPase subunit H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.150">
        <property type="entry name" value="V-type ATPase, subunit H, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004908">
        <property type="entry name" value="ATPase_V1-cplx_hsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011987">
        <property type="entry name" value="ATPase_V1-cplx_hsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038497">
        <property type="entry name" value="ATPase_V1-cplx_hsu_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10698">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10698:SF0">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11698">
        <property type="entry name" value="V-ATPase_H_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03224">
        <property type="entry name" value="V-ATPase_H_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF032184">
        <property type="entry name" value="ATPase_V1_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="4 6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ATPase V1 complex subunit H C-terminal" evidence="7">
        <location>
            <begin position="335"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00046225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR032184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF11698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009486-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="475" mass="54679" checksum="62B1D7082B81F47F" modified="2020-08-12" version="1">MTDVQDIMSSLPDDKIDMIAATSVLQQQAGDIRQTKPNWSSYMQSQMISQEDYACVSSLDKDKKAQAAYLQENAPQCAKTLLNMLSHVSKDQTIQYILVLIDDLLQEDRTRVQIFHDYANKKKESVWAPFLNLLNRQDGFIVNMASRVVGKLACWGQEQMPKSDLHFYLQWLKDQLTVANNEYIQSVGRCLQMMLRVDEYRFAFVTVDGISTLISILSSRVNFQVQYQLVFCLWVLTFNPLLAEKMNKFNVIPILADILSDCAKEKVTRIILAVFRNLIEKPEDAQVAKEHCIAMVQCKVMKQLTILEQRRFDDEDITGDVEFLTEKLQNSVQDLSSFDEYATEVKSARLEWSPVHKSAKFWRENAQRLNEKQYELLRILVHLLETSKDPLVLSVASYDIGEYVRHYPRGKHVIEQLGGKQLVMQLLAHEDPNVRYEALLAVQKLMVHNWEYLGKQLEKDSEKATQGNAPISGKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W048</accession>
    <name>A0A6E8W048_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">E3 ubiquitin-protein ligase</fullName>
            <ecNumber evidence="10">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the UPL family. K-HECT subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="10"/>
    <dbReference type="EnsemblMetazoa" id="ACON009511-RB">
        <property type="protein sequence ID" value="ACON009511-PB"/>
        <property type="gene ID" value="ACON009511"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011695"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON009511"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030222"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009968">
        <property type="term" value="P:negative regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032436">
        <property type="term" value="P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070534">
        <property type="term" value="P:protein K63-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1750.10:FF:000033">
        <property type="entry name" value="E3 ubiquitin-protein ligase hecd-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2160.10:FF:000022">
        <property type="entry name" value="E3 ubiquitin-protein ligase HECTD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000051">
        <property type="entry name" value="E3 ubiquitin-protein ligase HECTD1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000085">
        <property type="entry name" value="E3 ubiquitin-protein ligase HECTD1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000014">
        <property type="entry name" value="E3 ubiquitin-protein ligase HECTD1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1750.10:FF:000044">
        <property type="entry name" value="E3 ubiquitin-protein ligase Ufd4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2410.10:FF:000007">
        <property type="entry name" value="Putative E3 ubiquitin-protein ligase HECTD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000372">
        <property type="entry name" value="Putative hect e3 ubiquitin ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045322">
        <property type="entry name" value="HECTD1/TRIP12-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010606">
        <property type="entry name" value="Mib_Herc2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037252">
        <property type="entry name" value="Mib_Herc2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012919">
        <property type="entry name" value="SUN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057948">
        <property type="entry name" value="TPR_TRIP12_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45670:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE HECTD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45670">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06701">
        <property type="entry name" value="MIB_HERC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07738">
        <property type="entry name" value="Sad1_UNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25579">
        <property type="entry name" value="TPR_TRIP12_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159034">
        <property type="entry name" value="Mib/herc2 domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51416">
        <property type="entry name" value="MIB_HERC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 9 10" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="repeat" description="ANK" evidence="8">
        <location>
            <begin position="421"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="8">
        <location>
            <begin position="452"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="domain" description="MIB/HERC2" evidence="13">
        <location>
            <begin position="1358"/>
            <end position="1430"/>
        </location>
    </feature>
    <feature type="domain" description="HECT" evidence="12">
        <location>
            <begin position="2591"/>
            <end position="3038"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="244"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="478"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="523"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1491"/>
            <end position="1519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1546"/>
            <end position="1594"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1617"/>
            <end position="1636"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1647"/>
            <end position="1698"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1828"/>
            <end position="1849"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1865"/>
            <end position="1884"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1906"/>
            <end position="2011"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="2079"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="2138"/>
            <end position="2162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="2217"/>
            <end position="2271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="2340"/>
            <end position="2361"/>
        </location>
    </feature>
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        <location>
            <begin position="2373"/>
            <end position="2423"/>
        </location>
    </feature>
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        <location>
            <begin position="274"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="480"/>
            <end position="502"/>
        </location>
    </feature>
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        <location>
            <begin position="531"/>
            <end position="540"/>
        </location>
    </feature>
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        <location>
            <begin position="1552"/>
            <end position="1564"/>
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            <begin position="1565"/>
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        </location>
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        <location>
            <begin position="1651"/>
            <end position="1698"/>
        </location>
    </feature>
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        <location>
            <begin position="1928"/>
            <end position="1942"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1975"/>
            <end position="2006"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="2080"/>
            <end position="2099"/>
        </location>
    </feature>
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        <location>
            <begin position="2142"/>
            <end position="2162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="2238"/>
            <end position="2255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="2352"/>
            <end position="2361"/>
        </location>
    </feature>
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        <location>
            <begin position="2393"/>
            <end position="2405"/>
        </location>
    </feature>
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        <location>
            <begin position="2406"/>
            <end position="2423"/>
        </location>
    </feature>
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        <location>
            <position position="3007"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006331"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU369009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009511-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="3038" mass="327506" checksum="C3E6950719DD8CC9" modified="2020-08-12" version="1">MGDVDPETLLEWLSMGQGDERDMQLIALEQLCMLLLMSDNVDRCFESCPPRTFLPALCKIFLDELAPENVLEVTARAITYYLDVSSECTRRIVAIDGAIRAICNRLEVADLESRTSRDLAEQCIKVLELICTREAGAVFEGGGLSCVLAFIRDSGSQIHKDTLHSAMAVVSRLCTKVEPSGANVQTCVESLSTLLQHEDPLVADGALKCFASVADRFTRKGVDPAPLAEYGLVRELLQRLSNAAGGPQISSSGGGGSAAISSSLGTNSSTHPESSPSAAQLSSSAPKSAQGAMEAGRSSQSIATTISLLSTLCRGSPSITHDLLRSNLLEAMERAFKGDERCVLDCMRLADLILLLLFEGRQALGRVGGSQGQLAPRVRRADSSTERTHRQLIDCIRSKDTEALIESIESGGIDVNCMDDVGQTLLNWASAFGTLEMVEFLCDKGADVNKGQRSSSLHYAACFGRPGIAKVLLKHGANPDLRDEDGKTPLDKARERPDEGHREVASILQSPGEWMMAATRSDVKCGDSGDETGGAGGVGEPRGDPEMAPVYLKFFLPTFCKTFQSTMLASVRRSSLGLIKKMIQYVQPEVLSKLCSSEGLQSYEQSLGTLLVEVIASVLDNEDDEDGHLVVLTIVQELMSKTQNDFLDHFARLGVYTKVQALMGEPSFDGSDNNDVIKSTSDDAKSAAAAACSSTDASGVVTVTPGAPTVTTASGGTASAAVAVEDAKEILHGKAYHWHDWSICRGRDCLYVWSDSAALELSNGSNGWFRFILDGKLATMYSSGSPENGSDSTENRGEFLEKLQRARAAVRQGTVSQPILSAPSLARIAVGNWVLQSQKEHQLHINNSEGHQVTILQDELPGFIFESNRGTKHTFTAETTLGPDFAAGWINTKKKKMRCKAEAQKYQLHKLARDLYNRYFKAAQAIPRGAVAKLSKIVHQIEIALEEQQSTSKAALISSTTQQITPPSAGVSWQEKLYNALTELVHLLNEDGVISAYEMYSSGLVQALVAVLSPNYWDLGMNRTKANKYQKQRLSIFKKCMYGGELKTGKNTAAILVQKLVAVLESIEKLPVYMYDSPGGSYGLQILTKKLSFRLERAACEQTLFDRTGRNLKMEPLATVGHLNKYLLKMVAKQWYDMERSSFLYLRKMKEAKPGTMQFRHRHDFDENGLIYYIGTNGRTLEWVNPAQYGLVTVTSSEGKQLPYGKLEDILSRDSVSVNCHTKDNKKSWFAIDLGIFILPTAYTLRHARGYGRSALRNWLFQMSKDGVSWVTLLTHTDDKSLAEPGSTCTWPIDCPADEQQGYRHVRIHQNGRNASGQTHYLSLSGFEIYGKVMSVCEDMDKTAAKENEAKLRKERRQIRSQLKYITDGARVVRGVDWHWDDQDGSPPGEGTVIAEIHNGWIDVKWDHGMRNSYRMGAEGKYDLKLANVDGLMAGGYDLHSSGISVTTVGGGANNNQFASTANVQCELADGGSTASGKKKVYDKSLNVLTSRKSSSTPSLPEATTENRSSVASTEQATSADNLSWKQAVEVITENVLSSARSDLATVGSGGSSNDLSSSVVTSSTAATGNNQEVSVTVHSSLSERGNNIPDLSQINSSTSMLVSDLATITENLSLSDGSAKQSGTATASSAGGQQFVSNISGTGVPVLMGSSSSSSSSSSTEENNKTNNINETNNKINLTSGSGASSVASGSSASSGKAGLSYLQTRLDMMGKMREGVDMLRNNTNNFLSSELLTQSNLLSSVKIAFPPIPPANATTGSSSSTTATTIPGSASYGSSIFVASTSTGTNPAVSSGTGAKTPTDKFDVKFNNTATANTFKKVLNEAKQIGAEQPPAPPAPASSMGGRDATNNLKNNIVVVGSTETTVLGGGGGGGSSQDDPNAMDCLMGSVSGSDGVNVVPVVAPSPNSMSVSVPNLTSSGTGANNHSHNVSHRTSSHHHHHHNHPDSASQTLANDAQPPPPGLLETFAAIARRRTSGSGSNSNSNANNNNENNNAATHPSSSSSSSSQQTTAAVPINNQLISSGAGALVGGGGGGVLQNNSNFFPRGPNSVTSLVKLALSSHTGLLSTAQSYPSLFSSSSNNNAAAGQGAGNNNNANNMVGVGQVNPLNPALTMSLTSTSSDSEQVSLEDFLEQCRAPTLLGDLEDDEDIEDENDDDENEDEYEEVGNTLLQVMVSRNLLSFMEERTFENRLPTAGKRKSWDDEFVLKRQFSALIPAFDPRPGKTNVNQTSDLDIPAPGSNSATDPTEQPQSSSSGRGALPEPGSGLSSTAISSLPQPTLSLILRGPNINGVNDVEVDLTQSDWTIFRAVQELMLQTTMPKQDKFRKIWQPTYTIIYREASPGSSSSLLGGGGKEDFSSGEEGRATPIISMYSQRSHGSTLSPSSPVPGTPSLSGGGGAGGTGASSGGVLSSAPGTGGNQQQQQQQYCSVEDVLQLLSQLNSINQSLAATPTNNDKNLMPDVESHYLSPDVFMSKKITNKLQQQIQDPLVLSSGSLPKWCEEYNQTCPFLFPFETRQLYFSCTAFGASRSIVWLQSQRDVSLERQRAPGLSPRHADQQEFRVGRLKHERVKVPRGENLLDWAQQVMKVHCNRKSVLEVEFVGEEGTGLGPTLEFYALVAAELQRSDLGMWLCDDEEPKLIEDEIDLGEGSKPVGYYVRRSTGLFPAPLPQDSDISEDVSGYFWFLGVFLAKVLQDNRLVDLPLSNSFLQLLSHSRSMARGATSQTGSLLGKSGVSDDLMMSSILSEDSDRDRDLLVDSYQSKMAMASDGAWYDGILTQENLQEIDPIRYQFLRELQELVQQKQAIEQNDALSSEEKLQQISELKLNTKTGCVALEDLALTFTYLPSSRNYGYASADLLPNGANIDVTINNVEEYCNLTVAFCLQEGIAKQLAAFHRGFCEVFALNKLAAFTPDEIRKMLCGEQNPEWTREDIMTYTEPKLGYTKESPGFLRFVNVLMGMNGSERKAFLQFTTGCSSLPPGGLANLHPRLTVVRKVDAGEGSYPSVNTCVHYLKLPDYPNEQILRERLLTATKEKGFHLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W065</accession>
    <name>A0A6E8W065_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8W065"/>
    <dbReference type="EnsemblMetazoa" id="ACON009935-RC">
        <property type="protein sequence ID" value="ACON009935-PC"/>
        <property type="gene ID" value="ACON009935"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011871"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON009935"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040987"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009935-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="76" mass="8972" checksum="CC0632A7B54AA438" modified="2020-08-12" version="1">SRPLHKLCEHEGKPLPAHYKSDCYNDVDESNYACKEKYRIMMRLNPPGSDAPFNGQRLSRFMDMDDDMKRIKSKKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W1U8</accession>
    <name>A0A6E8W1U8_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Kinesin-like protein unc-104</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Required for presynaptic maturation, has a role in axonal transport of dense-core vesicles carrying synaptic vesicle precursors, components required for the morphological transformation of axonal growth cones to mature boutons.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON010519-RA">
        <property type="protein sequence ID" value="ACON010519-PA"/>
        <property type="gene ID" value="ACON010519"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON010519-RF">
        <property type="protein sequence ID" value="ACON010519-PF"/>
        <property type="gene ID" value="ACON010519"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001094"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON010519"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037311"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904115">
        <property type="term" value="C:axon cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048490">
        <property type="term" value="P:anterograde synaptic vesicle transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021700">
        <property type="term" value="P:developmental maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051222">
        <property type="term" value="P:positive regulation of protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040012">
        <property type="term" value="P:regulation of locomotion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051960">
        <property type="term" value="P:regulation of nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010975">
        <property type="term" value="P:regulation of neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008582">
        <property type="term" value="P:regulation of synaptic assembly at neuromuscular junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd22705">
        <property type="entry name" value="FHA_KIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01365">
        <property type="entry name" value="KISc_KIF1A_KIF1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01233">
        <property type="entry name" value="PH_KIFIA_KIFIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.20:FF:000001">
        <property type="entry name" value="Kinesin family member 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000004">
        <property type="entry name" value="Kinesin-like protein isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000204">
        <property type="entry name" value="kinesin-like protein unc-104 isoform X6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2520">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022164">
        <property type="entry name" value="Kinesin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022140">
        <property type="entry name" value="Kinesin-like_KIF1-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032405">
        <property type="entry name" value="Kinesin_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049780">
        <property type="entry name" value="PH_KIFIA_KIFIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47117:SF10">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIF1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47117">
        <property type="entry name" value="STAR-RELATED LIPID TRANSFER PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12473">
        <property type="entry name" value="DUF3694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12423">
        <property type="entry name" value="KIF1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16183">
        <property type="entry name" value="Kinesin_assoc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="9 12" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="16">
        <location>
            <begin position="3"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="15">
        <location>
            <begin position="1542"/>
            <end position="1640"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="391"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="952"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="359"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="617"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="398"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="968"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="97"/>
            <end position="104"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00056070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010519-PF"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1692" mass="190011" checksum="1F0272D4BC0A7257" modified="2020-08-12" version="1">MSSVKVAVRVRPFNSREIARESKCIIEMAGNTTCITNPKVPPGSSESVKRFNYDYSYWSHDPRDLEFSTQAMVYSDIGEEMLQHSFDGYNVCIFAYGQTGAGKSYTMMGKQEDGQEGVIPMICKDLFRRIQETESDDLKYSVEVSYMEIYCERVRDLLNPKNKGNLKVREHPLLGPYVEDLSKLAVTSYQDIHDLIDEGNKARTVAATNMNETSSRSHAVFTIFFTQKRQDRMTSLETEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEIASKNKKSKKADFIPYRDSVLTWLLRENLGGNSKTAMIAAISPADINYDETLSTLRYADRAKQIVCKAVVNEDANAKLIRELKEEIQKLRELLKAEGIEVQEGEDGEKKIHSPNRNRKRTGSSTEMAVDQLQASEKLIAELNETWEEKLKRTEQIRVQREAVFAEMGVAVKEDGITVGVFSPKKSPHLVNLNEDPTLSECLLYYIKDGLTRLGTSEANVPQDIQLSGSHILKEHCVFENKDGVVTLVPHKDALVYVNGRKVIEPEVLQTGSRVILGRNHVFRFTHPEQAREKREKNKEVDVCETPGNSGEIADWNFAQCELLEKQGIDLKAEMEKRLLALEEQYKREKRAADQEFEEQRKTYEARIDALQKQVEEQSMTMSMYSSYSPEDFHQEEDIFVNPLFESCWTAREAGLAAWAFRKWRYHQFTSLRDDLWGNAIFLKEANAISVELKKKVQFQFTLLTDTLYSPLPPELTPAPAVGALTNGGQEDEFGQNPIPRTTVAVEVTDTKNGATHYWSLEKLRQRLELMREMYHNEAELSPTSPDYNVESLTGGDPFYDRFPWFRMVGRSFVYLSNLLYPVPLVHKVAIVNERGDVRGYLRVAVQPVMDEENADFSNGVKQSARILFDEEQNGQHKVPKIRTIPDKDEKYIEGGNEMGTKLEELEQEDADSGRGDSSVASELHESNDQEPGEHLQPGKEFTFRVTVLQATGIAAEYADIFCQFNFLHRHEEAFSTEPVKNSGSGAPLGFYHVQNITVPVTKSFIEYLKTQPIVFKVFGHYQHHPLHKDAKQDCQITRPPPRRMLPPSIPISQPVRSPKFGPLPCPPSSTVLAKHDVLVWFEICELAPNGEYVPAVVDHSDDLPCRGLFLLHQGIQRRIRITIVHEPTPEVKWKDIRELVVGRIRNQPEPADDEDSDSCVLSLGLFPGEVLEVPGDDRSFFRFEAAWDSSLHNSALLNRVTQTGEQIYITLSAYLELDNCARPAIITKDLSMIIYGRDARTGPRSLKHLFSGQYRNPEANRLSGVYELSLRRASEAGSPGVQRRQRRVLDTSSTYVRGEENLHGWRPRGDSLIFDHQWELEKLTRLEEVGRVRHLLLLRERLGMDTTPNPTTKTEKDVCNLAQRASASPVHMVIPPSPQTPVKDQQAPAMPERELSVRETELVWKCVKLIQGRIGCKELGAEGNGSGTQASDASPGDEGCADMNASYISSNSIELCSPERVDMPNGWEAPAPAPQTQDLSLRLYVPELEEIRVSPVVARKGYLNVLEHGGSGWKKRWVTVRRPYVFIFRSDKDPVERAVLNLATAQVECSEDQAAMVKVPNTFSVVTKHRGYLLQTLGDKEVHDWLYAINPLLAGQIRSRLARKNISSISSTGSGAGDQQQATTGASALAVAAAQGNGGGSSSGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W1U9</accession>
    <name>A0A6E8W1U9_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the DOCK family.</text>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON010192-RA">
        <property type="protein sequence ID" value="ACON010192-PA"/>
        <property type="gene ID" value="ACON010192"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006787"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON010192"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039551"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08696">
        <property type="entry name" value="C2_Dock-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11695">
        <property type="entry name" value="DHR2_DOCK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.740:FF:000002">
        <property type="entry name" value="Dedicator of cytokinesis protein 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.410:FF:000002">
        <property type="entry name" value="Dedicator of cytokinesis protein 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037808">
        <property type="entry name" value="C2_Dock-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027007">
        <property type="entry name" value="C2_DOCK-type_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026791">
        <property type="entry name" value="DOCK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021816">
        <property type="entry name" value="DOCK_C/D_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043161">
        <property type="entry name" value="DOCK_C_lobe_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043162">
        <property type="entry name" value="DOCK_C_lobe_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027357">
        <property type="entry name" value="DOCKER_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046769">
        <property type="entry name" value="DOCKER_Lobe_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046770">
        <property type="entry name" value="DOCKER_Lobe_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046773">
        <property type="entry name" value="DOCKER_Lobe_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23317">
        <property type="entry name" value="DEDICATOR OF CYTOKINESIS DOCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23317:SF76">
        <property type="entry name" value="LD20667P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06920">
        <property type="entry name" value="DHR-2_Lobe_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20422">
        <property type="entry name" value="DHR-2_Lobe_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20421">
        <property type="entry name" value="DHR-2_Lobe_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14429">
        <property type="entry name" value="DOCK-C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11878">
        <property type="entry name" value="DOCK_C-D_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51650">
        <property type="entry name" value="C2_DOCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51651">
        <property type="entry name" value="DOCKER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2 DOCK-type" evidence="5">
        <location>
            <begin position="574"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="domain" description="DOCKER" evidence="6">
        <location>
            <begin position="1606"/>
            <end position="2041"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="212"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="411"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="417"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010192-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="2065" mass="233914" checksum="2530BC4F28BE7E13" modified="2020-08-12" version="1">HNSSDIRKNVANYQLLSKSDSSSVCSSSISLCDLVEPIDYEEFLAQHSTLLQKDHLRSILDFPSGDVQVKIVPRKIRTTEHVIPKEPMEVLPLYVQHCVDCFTRPWKVVEFSARHHSSSCNARERKDKGALSPTSYQQEFEIDRDFSNLVSLEESVTSTTLYASESCTPSSRQSIASLSSVSTCTDTLTPRGSWASFDLRSSVNDPLITGVLERVPPESVDQSNESKRQEERQPALFGLYADGETEEAIEKRMPADMPMEHLGNRIHVKCLHLKLELEVEPIFASMAIYDAKEKKKLSENFYFDMNPESLRRMLVNHVPFADISTQAREAIFDITNPSNELYLVIRLEKVLQGDIKDSVEPYLKDDKDKYKDKAKSNAADYCERLGKYRMPFAWTGIYLTGIFNGDGMEKDDRESIGSASSSNSLDRKSSTSSFDQFRKRATDMGTLTRRGSLERKMEKRRSWSPDDFANSIETFRPITITVASFFKQESDKMKDEELYKFLPELKRPAALMKKHKCIPGSIKVEIAPAPEELKYALTPELAKIDPYPDDHSRPVKEILEFPSTPILNPYYAYRNLLFVSPKELNFSTRAGSARNIAVRVQLMGGEKQSDALPAIFGKSSCSEFSTEAYSAVNYHNKQPTFYDEIKIELPANLKQNHHILFTLFHVSCQKKPQEVQTTVETPVGYTWLPVLKDGGHLNVGEFNLPVMVEEPPNNYSFIPPDVQLPGTKWLDNHRQVFSVTIDAVTSVHALDDYLDKFIYLCECLDMRKVPPRIGEGNMEKEFKKTLQELQSADQEQLVKNLQVILDKLIELLVTSYKIGGEALSLGQTVFETICQISDKIFFLQPDERYGRQNILSTYIQYQCKIPHPMDPKCKLASLQMPIRRPASAEEINRSTSNPDLMALSDLNSSRTVDRALSMRSDGCSPGSGVKDGLVRLLHEEIALNWVVASGSAAELSMTNSWMLFELIVKSMVEHLELTNALNSPRKSRFPHQYTDDIATLVHLVTTKVVGYNSSDQKLAQSINSSLAFFIFDLLSIMDRGFVYGLIKTYYKVIMTKSSPTPDMIQYKLDFLRIVCSHEHFVALNLPFGTPYTVLSAPCSPTPSVTSNHSQNSYISAMTGNDRALYADLSVEFRQQHFLVGLVLQELSNVLDISNTQLHGKAIRCLRNLLTSHDLDPRYSETDARARVAALYLPLLGIVMDVIPQLHTPVSQSHDRLNTIGQLDDYQGPAATVPATSTISPEVAFAISGIRRYSYVSETPKPKTVLTNDTRNLLACFLWVLKNLEPNTLVKYTMGLSPHRVHQMLQVLNICIPNFEYRGKKQPTNTSKRNTSSFRKTPDMREKLEEFIRGTGSARNDLINRRKDGRNSTDKLRWKKDQIRTQFYDSNIKNEAELEMCNYIEGSLATEVCLIILDTLEMIVQVASSSEMHHNLLGTVLKVLLHALSRNQSTLALQNLFASQRSLVFKYHNLLFDEETDNCADLCLLLLKHCGSQLPTVRSQAAASLYLLMRQNFEIGNNFARVKMQVTMSLSSLVGTSSSFSEQSLRRALKTILVYAESDTDLQETSFPEQVQDLLFNLHMILSDTVKMKEYQEDPEMLLDLMNRIAKGYQNSPDLRLTWLENMAKKHTERANHTEAAMCYVHSAALVAEYLSMLESQTHLPVGAVSFKHISPNALMESAVSDDVVSPGEDGICLGNSFTEGGLKQLLDHAASAFQAAGMYEAMNDVYKVLIPICEANRDFRKLAQIHGKLLEAFNRIAQLQGKRVFGTYFRVGFYGAKFGDLDQQEFIYKEPTLTKLPEIFSRLQNFYADRFGPDVVQIIKDSNLVEISSLDPDKAYIQITYVEPYFETYELRYRETYFERNFNIKRFIFATPFTKSGKAHGDLHEQCKRKTILTTANHFPYVKTRIQVVQRQQIVLEPIEVAIEDIQKKTAELAAATTQEPADPKILQMVLQGCIGTTVNQGPMEMALVFLSNIANGNTIPTKHQNKLRLCFKDFSKKCADALKKNRNLILSDQKDYQKELERNYQVFTERLAPLITISPAQAQGLVAVKNDLHNNNRLTCSLRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W258</accession>
    <name>A0A6E8W258_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Neurotransmitter-gated ion-channel ligand-binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8W258"/>
    <dbReference type="EnsemblMetazoa" id="ACON010169-RB">
        <property type="protein sequence ID" value="ACON010169-PB"/>
        <property type="gene ID" value="ACON010169"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007447"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON010169"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_029493"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005230">
        <property type="term" value="F:extracellular ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18989">
        <property type="entry name" value="LGIC_ECD_cation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="1">
        <location>
            <begin position="15"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010169-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="220" mass="25500" checksum="C197E38F57739F7F" modified="2020-08-12" version="1">SINCDKKRDNVQYTIKQHLFCNGYDPKIRPAKSEFDSINITTYAVLYSFDISEYRSVMSFQVNYYMRWQDSSLAWNASDWSNITTLNINNDEIWSPQFENINSDYEGQPSVSCLNPDCLLRVNGYVSCSPVCRISAKCSSDYSRWPFNTLVCRMWFTNRDKELVDEVNFLPVITYLPSNRHLPTTKWCVTSISSNKTQLIIPGATSRTVEDHTCFVSFFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W267</accession>
    <name>A0A6E8W267_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Adenylyl cyclase-associated protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CAP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8W267"/>
    <dbReference type="EnsemblMetazoa" id="ACON010175-RC">
        <property type="protein sequence ID" value="ACON010175-PC"/>
        <property type="gene ID" value="ACON010175"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000190"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON010175"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042252"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008179">
        <property type="term" value="F:adenylate cyclase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019933">
        <property type="term" value="P:cAMP-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.330:FF:000001">
        <property type="entry name" value="Adenylyl cyclase-associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.70:FF:000001">
        <property type="entry name" value="Adenylyl cyclase-associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.330">
        <property type="entry name" value="Adenylate cyclase-associated CAP, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001837">
        <property type="entry name" value="Adenylate_cyclase-assoc_CAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013912">
        <property type="entry name" value="Adenylate_cyclase-assoc_CAP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017901">
        <property type="entry name" value="C-CAP_CF_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016098">
        <property type="entry name" value="CAP/MinC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036223">
        <property type="entry name" value="CAP_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028417">
        <property type="entry name" value="CAP_CS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053950">
        <property type="entry name" value="CAP_N"/>
    </dbReference>
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        <property type="entry name" value="CAP_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006599">
        <property type="entry name" value="CARP_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003124">
        <property type="entry name" value="WH2_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10652">
        <property type="entry name" value="ADENYLYL CYCLASE-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10652:SF0">
        <property type="entry name" value="ADENYLYL CYCLASE-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08603">
        <property type="entry name" value="CAP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21938">
        <property type="entry name" value="CAP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00673">
        <property type="entry name" value="CARP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69340">
        <property type="entry name" value="C-terminal domain of adenylylcyclase associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101278">
        <property type="entry name" value="N-terminal domain of adenylylcyclase associated protein, CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51329">
        <property type="entry name" value="C_CAP_COFACTOR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01089">
        <property type="entry name" value="CAP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51082">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WH2" evidence="7">
        <location>
            <begin position="420"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="domain" description="C-CAP/cofactor C-like" evidence="8">
        <location>
            <begin position="475"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="380"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="66"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="391"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010175-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="641" mass="69492" checksum="87DE54404CDB7EB4" modified="2020-08-12" version="1">MEPPTTPIGRDELALRRHRFFSELLNAAHAAVEHRVRFDPLGPEVAKVATEEESEDCDPDLKPSSARELECLIDRLERIVDRLERTVSARELEETRRILKDACAVGKAVIRSNTTVEDTDPSADDLPTVLPSTPPPSASYLHQKVDSLESTLFPALTDQPQKGRQHNTTTVIQFKPSALLNHTNPLTIQEQGTAVLSLSSSQHGTVGGTTSKMSINAYEDIMLGSFANFIALSNKIGGDVATQAQFVKEAFDAQFAFLKVASASSAPSNTDLQNLLKPTSDKISTIQSYREKNRTSPFFNHLSAISESIPALGWVCVAPTPGPYVKEMNDAGQFYTNRVLKDWKEKDSTHVEWARAWIQTLTELQQYIKQHHTTGLVWSGKDKPTVGGASVPPPPPPGGLPPPPPMMPLGDLSADGSGDDRSALFAQINQGADITKGLKKVTADMQTHKNPSLRSGPAPYKAPAGVTNGTKSVAPPAAAAVAKPPTFTRDGKKWLIEYQKNNPDLVVENAEMNNVVYMFRCEGSTLQIKGKINSVVMDSCKKCSLVFDSLVASAEFVNCQSVQMQVLGKVPTISIDKTDGCQMYLSADSLAVEIVSSKSSEMNVMIPKGSSGDYTEQPIPEQFKTIVKGSSLNTTCVESLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W289</accession>
    <name>A0A6E8W289_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8W289"/>
    <dbReference type="EnsemblMetazoa" id="ACON010194-RB">
        <property type="protein sequence ID" value="ACON010194-PB"/>
        <property type="gene ID" value="ACON010194"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_013593"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON010194"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039884"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0089701">
        <property type="term" value="C:U2AF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12540">
        <property type="entry name" value="RRM_U2AFBPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000318">
        <property type="entry name" value="Zinc finger CCCH domain-containing protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009145">
        <property type="entry name" value="U2AF_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12620">
        <property type="entry name" value="U2 SNRNP AUXILIARY FACTOR, SMALL SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01848">
        <property type="entry name" value="U2AUXFACTOR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 8" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="11">
        <location>
            <begin position="160"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="10">
        <location>
            <begin position="192"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="8">
        <location>
            <begin position="160"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="7"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="20"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010194-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="308" mass="36799" checksum="B4F993C1B650C99A" modified="2020-08-12" version="1">HKEWRKLFKKKRRKAVRQKTAQERDRQNAEKEKQKLSCPEYQLYLAEKGRQEQIAAEREELERALRHAVWLEEERKAQAKFEKVRQEEEAKEREAREKREKIRKEYEERETKLKQAKEERFQQQEHARRMLHERFIKLQEFAATGADDYLSELQSIQHTRADAEDCKFFLKTGACRHGYRCGGNHPTPGVSQVILIPNFFSHPALEQAVHAEYGHDARLEFDEDDLKNSYNEFFRDIIQEFEMFGTVRHIFVCRNSVAHLRGSVYIEYESMRNAAAAYLRMNGRFYAKKQLHVEFRNTLTWPTAVCGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W3X7</accession>
    <name>A0A6E8W3X7_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Centrosomal protein of 135 kDa</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
            <location evidence="1">Centriole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CEP135/TSGA10 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8W3X7"/>
    <dbReference type="EnsemblMetazoa" id="ACON010985-RB">
        <property type="protein sequence ID" value="ACON010985-PB"/>
        <property type="gene ID" value="ACON010985"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009583"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON010985"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041654"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051877">
        <property type="entry name" value="Centriole_BasalBody_StrucProt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20544">
        <property type="entry name" value="CENTROSOMAL PROTEIN CEP135"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20544:SF0">
        <property type="entry name" value="NUCLEOPROTEIN TPR_MLP1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="104"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="140"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="754"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="221"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="108"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="140"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="207"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="763"/>
            <end position="773"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010985-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="789" mass="90134" checksum="C6EAE14442E46E36" modified="2020-08-12" version="1">MLEHDRKNSDLQVKLQQSQVRVRELESLLELCGPGAGKIPPLSDSSISSSPCPSDAMLQTALQQATDEKRLLYKQLNELKDREQSLLADFEKVKTKYTRLKQKHAALEQTQHHQPVSSSAESQVELQSLQYRCSELEEKLRHTKQERDRYSSDAERQRTIVSQLKRESVEKDHELAELKNELHAQRKSNRPSTASQGGGGRLRTADSHGSGQSSLSVQAAIHRIERERDAAKSEVRQLEQERDALREKLKLSTRSQQDAVAKHENAIAEYSGRVATLEAEKRDLQGGQSAAQMKVKLLKEESRELQARVKELEESYSKLKLSYSQMKILHEQTERALAQHQQRLLSTETQLGTAESKLQRVDSTVEDAQNEVGRLKGEISLLRASNASLVREKDKLLMDLDKKTENLYAAEAEIAELKAKRKELKSTIDRMQQKLDNVSTDNIHKESTLRSVSTETDTLKQQMATLKRKNDNASTENGRLSNELTDALAELTLTKRQLKDSQQEVERMKTQLREYVQEMQRAEELLLEKERERESMLERFKSLSEGVNVLETSNHTLEAETSEARKLLQEAEERIATLEELTNEREQNIRECEHQINELSASLAAAESELEALREENKALALDLEATKELCGKLDLQKDKLQAELEEHSNIREQLAREKGTLQRELTLTRTGDRAAVDGLQELLTASRADLEQHRLALAQQQQETEKLRTEVDALRSRMADEQDKARRSEALASEYGVQLQELRRQLTDERFALMRSRAGESQTEREDIHHEDEGDDGDDDDCNRYSTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W3Z3</accession>
    <name>A0A6E8W3Z3_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="3">PDZ domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="EnsemblMetazoa" id="ACON011384-RA">
        <property type="protein sequence ID" value="ACON011384-PA"/>
        <property type="gene ID" value="ACON011384"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011771"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON011384"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030583"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11324">
        <property type="entry name" value="IL16-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11324:SF16">
        <property type="entry name" value="PDZ DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="2">
        <location>
            <begin position="1138"/>
            <end position="1223"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="2">
        <location>
            <begin position="1325"/>
            <end position="1397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="147"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="200"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="280"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="575"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="623"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="906"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="933"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="987"/>
            <end position="1025"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1062"/>
            <end position="1106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="66"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="585"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="626"/>
            <end position="635"/>
        </location>
    </feature>
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            <begin position="656"/>
            <end position="670"/>
        </location>
    </feature>
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        <location>
            <begin position="671"/>
            <end position="691"/>
        </location>
    </feature>
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        <location>
            <begin position="699"/>
            <end position="731"/>
        </location>
    </feature>
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        <location>
            <begin position="906"/>
            <end position="915"/>
        </location>
    </feature>
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            <begin position="940"/>
            <end position="960"/>
        </location>
    </feature>
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        <location>
            <begin position="1062"/>
            <end position="1079"/>
        </location>
    </feature>
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        <location>
            <begin position="1088"/>
            <end position="1099"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON011384-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1408" mass="147724" checksum="1AA5297806D1A0C0" modified="2020-08-12" version="1">MEPRVIALKPKHAGLGIATESGHIASSGLATGIVDSSSEGLTVFANNNNNNNAKNHSVTDADSDKSSTAAAAAPGSVTSMDSMNNVVTTTTTVTAIASTVHQLIPSAAPSAAAAAPTAPTNGGKVVAAAPPAFSRLPPDGHEFPPNFTEPTTTTTTTATNGNSSSGVNGPTTVGPVTAMTIMNGAGVTVVAAPPVLAKSNDKHSSFNDDLPDLPKSHPPPVPRKVTTAANAGDTFRKPPPFPTRSSSIAESMLSRKELPQAYQSLQSVFEQRRRSVDVPDHNGKAAAPSLPPSGRISLVGSNWRKDEKSEKSVRDKIAMFSSAADAEGGVAPVPQQHAATTTAANNPSALLPASRKFSKDFTKSSENLLGSSARDTGSRKALEAIETYATLRKKAHSVEHLDEVDRGVAASTATVANETPYKAKFSLETSKPVVLGKAYSVENLNPANRSSDTVLKPSTNGIENGSKILSRTTSFSGYSNGNSLATIASSPAASVGGSTNSLTALQNNTPAASDERWKSSISSLLEQRKKSMSKLRGLVIPEKVPEAEVLPETRIIGLPIIKSKDSEIILSSAKPPSAATDAYSRRTSTLPMPPKTSLSSLTASTIVARSVAAIEPELPAFRKPLVVPPLPPAKPPRTSLVYPPKSGSTDCLRSTEDDDESDDGDGDESDSVLSSRVSSPPVSPVAPAAPVEKYALTRTLSSETNTSITSSNSTLTSSSGSQASCSSVGSTPTVDISRKLSKSSSSEASMNRKNVLASAKSRNGRGDLKIEDVVGNGKSKRYEDGDSTDGYEEEERRKSKSKPRSSLTNGKGASYEQERKELTHYKVVDSVDGSSIVDKVIKVASYVEVVSDLEDSSIVEEPIVASSTVAVPVVTEKLPAEKKISHENGGSMSDLAKWVRHEAARTISHKPEAESNSKTGTPTVGKIVGALGARGERKQQPPPPTPPRTVPPPPTEPVPVPVRRELRSSVETKKLNLAEIRKSFESKSANSTIIPTPGKQPVMKEQQHHHAAPPATTPPTAPSVAAVPAPQTAVVAPPATPPVHATPKTTTNNGHDRFSSWDSLASSSSGVSSLQTNSLGMGRGAPNCTGSSQTLQSTPSDYGSFSSLGSSHSLITPQDLQLIIEEADPPLATPEAFVVVLQRETPESSIGITLAGGSDYEAKEITIHKILTNSPADRDGRLRKGDRILSINGLSMRGLTHRESLSVLKTPRPEVVMVITRSKSLVLDTLTKLKRPSMGSLSSLAEKSEIATEYERKLKIQHKASRSLDLDHLDVASNEAESVFDGTASEDGLLSADDVSKCSSSNATDNASSYSTAEVPEGCRMVEINKDGAGLGFSIEGGYDSPAGNKPLIIKKIFMGGAAEKSGLLRAGEEIVAINDISIAKMTRIQVWNMMKKLPNGGVRITLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W3Z4</accession>
    <name>A0A6E8W3Z4_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="7">C3H1-type domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8W3Z4"/>
    <dbReference type="EnsemblMetazoa" id="ACON010800-RA">
        <property type="protein sequence ID" value="ACON010800-PA"/>
        <property type="gene ID" value="ACON010800"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009442"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON010800"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032179"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052647">
        <property type="entry name" value="Zinc_finger_CCCH-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041367">
        <property type="entry name" value="Znf-CCCH_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46582">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46582:SF1">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18044">
        <property type="entry name" value="zf-CCCH_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="501"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="4">
        <location>
            <begin position="501"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
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            <begin position="1"/>
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        </location>
    </feature>
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        <location>
            <begin position="646"/>
            <end position="973"/>
        </location>
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        </location>
    </feature>
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        <location>
            <begin position="202"/>
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        </location>
    </feature>
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            <begin position="219"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="248"/>
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        </location>
    </feature>
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        </location>
    </feature>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="359"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="392"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="432"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="445"/>
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        </location>
    </feature>
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        <location>
            <begin position="475"/>
            <end position="493"/>
        </location>
    </feature>
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        <location>
            <begin position="494"/>
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        </location>
    </feature>
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        <location>
            <begin position="648"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="770"/>
            <end position="781"/>
        </location>
    </feature>
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        <location>
            <begin position="787"/>
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        </location>
    </feature>
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        <location>
            <begin position="820"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="864"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="907"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="945"/>
            <end position="967"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010800-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="973" mass="104172" checksum="D0C82C8368C5934C" modified="2020-08-12" version="1">MDSDDSRPGSSASNSSDSEQAASPGGGKEQQEDDHSDGSGSPSRSPSPASNAGDVLGGGDGGGDDDGGEPNDGGRESNRPSRSRSRSQSRSNSPGSLSYDAGKQDSLSSPAKTFDAVNSPSYLSAENSPQSHPKSPASSSAHNRSSRSPSGGSHGERRYDRSRSLSKESNHSDNSSSKGGSHHHRRRGGSASSSNRQHRSLSRNSTTSNLSEKLKGEEISDCEMEEESDREDKAPASPEAGGDERGVEGGAVAGSDGGPGAKNSALNMSHEDLSDVSLESDNEPGRAGEDEEEEDGEEEDDRDSERESVKRHAGGRKEHDGTDEEQEEGEEDDEHSRGPATSTVSASERDGSEAGEQQQQRDERPKITDLRQKLEERKNQLRNMDADKHTINGGGGGVGGASGANGGGAAVARNHDKLLHEGNLMDSKNDEDALDFEAEEGECNDAPKEPEKKADDATEKGKKEEAKEGAAAAGGDDELEEGEELEEGEVTDEDEKRPEESEPKPVCRFYTRGQCTWGMSCRFLHPGVTDKGNYTMFDMVRPVPVTHQPPGYGGHPAAPLYPPDYRHERPPLHHLAHHPVAVVAPYPATVGLPPPVRPVEPVVAESAWERGLRTAKEMMRKANKRKEQDIDFDDKKMNLTLTQDELEKDNYYTRDRASPSDITPAYPGRPPAAVPPGYDYPAPTSKYPRGGAGGGAAGPGAPPFEEDAFGRTHRYRELPSHRMPQYEDDDLSKRRHPRATREVILQRADEWNDPWMRSKSPGRDKRGGRRGGGGGGGGGGGADRRSYSNSSSYSSSSSSSRSGSSSDSSRSPSPTTTRRNRYDSHRSKDGERHASTTHVRKVIRTRSPSVSPRRGRKGSPLPVKRMDKRGPSPVVEKRMVEKVVTSKRKMSPPMHKASAAKHRRHSSSSGSGSSDSSDSGSESSYSSSSSSSSRGAGKGRTAKVRASEKSSKSASMGKKDSKKHSEGKGGGAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WA32</accession>
    <name>A0A6E8WA32_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">MAP/microtubule affinity-regulating kinase 3</fullName>
            <ecNumber evidence="5">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Serine/threonine-protein kinase par-1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="27 28">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="27">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Serine/threonine-protein kinase. Involved in the specific phosphorylation of microtubule-associated proteins for MAP2 and MAP4. Phosphorylates the microtubule-associated protein MAPT/TAU. Phosphorylates CDC25C on 'Ser-216'. Regulates localization and activity of some histone deacetylases by mediating phosphorylation of HDAC7, promoting subsequent interaction between HDAC7 and 14-3-3 and export from the nucleus. Regulates localization and activity of MITF by mediating its phosphorylation, promoting subsequent interaction between MITF and 14-3-3 and retention in the cytosol. Negatively regulates the Hippo signaling pathway and antagonizes the phosphorylation of LATS1. Cooperates with DLG5 to inhibit the kinase activity of STK3/MST2 toward LATS1. Phosphorylates PKP2 and KSR1.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="19">Interacts with MAPT/TAU. Interacts with DLG5 (via coiled-coil domain). Interacts with STK3/MST2 and STK4/MST1 in the presence of DLG5. Interacts with YWHAB, YWHAG, YWHAQ and YWHAZ. Interacts with PKP2 (via N-terminus). Interacts with CDC25C. Interacts with KSR1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cell cortex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="5"/>
    <dbReference type="EnsemblMetazoa" id="ACON029469-RC">
        <property type="protein sequence ID" value="ACON029469-PC"/>
        <property type="gene ID" value="ACON029469"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_014020"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON029469"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032840"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034589"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050321">
        <property type="term" value="F:tau-protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12196">
        <property type="entry name" value="MARK1-3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14072">
        <property type="entry name" value="STKc_MARK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14337">
        <property type="entry name" value="UBA_MARK_Par1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001032">
        <property type="entry name" value="KP78b, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000005">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000003">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.80:FF:000001">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.80">
        <property type="entry name" value="Kinase associated domain 1, KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028375">
        <property type="entry name" value="KA1/Ssp2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001772">
        <property type="entry name" value="KA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049508">
        <property type="entry name" value="MARK1-4_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF82">
        <property type="entry name" value="KP78A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02149">
        <property type="entry name" value="KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103243">
        <property type="entry name" value="KA1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50032">
        <property type="entry name" value="KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 22" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 22" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="24">
        <location>
            <begin position="429"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="25">
        <location>
            <begin position="699"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="domain" description="KA1" evidence="26">
        <location>
            <begin position="1129"/>
            <end position="1178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="28"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="86"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="169"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="241"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="300"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="766"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="851"/>
            <end position="1013"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="1044"/>
            <end position="1078"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="1183"/>
            <end position="1362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="29"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="90"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="177"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="23">
        <location>
            <begin position="323"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="337"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="23">
        <location>
            <begin position="361"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="785"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="851"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="871"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="899"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="936"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="962"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="975"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="1002"/>
            <end position="1013"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="1046"/>
            <end position="1059"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="1063"/>
            <end position="1074"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="23">
        <location>
            <begin position="1186"/>
            <end position="1199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="1311"/>
            <end position="1328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="23">
        <location>
            <begin position="1329"/>
            <end position="1354"/>
        </location>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="458"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00054424"/>
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    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00063680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00071529"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00074935"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
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    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
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    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50032"/>
        </source>
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        <source>
            <dbReference type="EnsemblMetazoa" id="ACON029469-PC"/>
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    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1375" mass="145903" checksum="0E2517D090667903" modified="2020-08-12" version="1">MSASKEFERNEAIRKSIRLKVPIRMEVAGTGATTTTTSTTTTATSPAPPPITSPTGTRRPVSLMTSPLPSPRLATLGVRERNLRNELDSGPTQRTSYLQRPTPAAKPATARVDIRDGYSLKLSDITRAPAEQPNSESVDVHSLSVLQTLERQINTMEFDERMKQRRDLLLQPPGSPGSPIMSSASMARRKDQRPVHYGELPSLLNTGISDVATGNGFGYLDREDSGANDDSDDELAHARQNAFKRGEEGRSSTGGATGYSMKPKATRIINRTVSDTKNAEAAVKTIRLKKLAPKPPVAVVGMKPSTAAPPPASSTTAADKGKGGLGLVVGRGGTSGGTAATVVGSSSATGSTSTTASSSSGSGGTTAAGSGGGVTGSKPAAITTEKKISAEVLKQFAAAQTQKVQGSPNMQMRGTPARWRSGEEHIGKYKLLKTIGKGNFAKVKLAKHVPTNKEVAIKIIDKTQLNPSSLQKLYREVRIMKMLDHPNIVKLFQVIETEKTLYLVMEYASGGEVFDYLVAHGKMKEKEARAKFRQIVSAVQYCHQKRIIHRDLKAENLLLDSEMNIKIADFGFSNEFTPGSKLDTFCGSPPYAAPELFQGRKYDGPEVDVWSLGVILYTLVSGSLPFDGATLKELRERVLRGKYRIPFYMSTDCEVLLKKFLVLNPSKRANLETIMKDKWMNMGYEDDELKPYVEPLPDLKDQKRIEALVAMGYNRQDIEDSLANTMYDDVFATYLLLGRKSTDSESDGSRSGSSLSLRNIAGNEGAAAGNSQVQSPTHRGVHRSISASSTKPSRRASSGGETLRVGPTTAVAAAAAAAAVGAAAGGGAGTGTVVGGGTGGGTANVTGNSVLAGATNNHSSGTGGSGGGASERTSISSNFKRQNTIDSATIKENTARLAAQNQRPASSITKPITSVDNSSISSPAKARTTSSSTSTKYDPSNGSRTVGPSAGLMPRRSTTLYEKTSSTEKTNSTTDPTMVNRNSLTPAVNSSRQPVAFPRNVPSRSTFHSGQTRARNSTVYAGTGGNVGDSPHSGKTFLQRLTTRFSKRDNLRLPSERPNDGQANTSTSTASTNTEEPVKPRVLRFTWSMKTTSPRLPDEIMAEIRSVLDKNNCDYEQRERFVLLCVHGDPNTDSLVQWEIEVCKLPRLSLNGVRFKRISGTSIGFKNIASKIAYDLQYHSVGSDTTQDDGDDGGDDDDGTASGGGGGLTEAESESEQKRSNHHHHARKEGGNGGDGSVEPVEPFVPGGSVRMRSKTPSAAGATGGGSKRWSLVVVDGRSSLRRKEKERISSHHQQSLPRQRKEKMKEEDTGPTTTTTPSRTETVATTSGNGGGGEGEGSGDGGGGGGGGGGSTGTGCQPAGYNSLFASLLRTTTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WA37</accession>
    <name>A0A6E8WA37_ANOCL</name>
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        <recommendedName>
            <fullName evidence="5">Protein FAM89A</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM89 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8WA37"/>
    <dbReference type="EnsemblMetazoa" id="ACON029474-RA">
        <property type="protein sequence ID" value="ACON029474-PA"/>
        <property type="gene ID" value="ACON029474"/>
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    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007484"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON029474"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033292"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039499">
        <property type="entry name" value="LURA1/LRA25"/>
    </dbReference>
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        <property type="entry name" value="AT07979P2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46949">
        <property type="entry name" value="LEUCINE REPEAT ADAPTER PROTEIN 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14854">
        <property type="entry name" value="LURAP"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="24"/>
            <end position="109"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="123"/>
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            <begin position="201"/>
            <end position="333"/>
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            <begin position="26"/>
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            <begin position="37"/>
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            <begin position="213"/>
            <end position="222"/>
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            <begin position="223"/>
            <end position="232"/>
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            <begin position="233"/>
            <end position="268"/>
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            <begin position="276"/>
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            <begin position="296"/>
            <end position="317"/>
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            <begin position="318"/>
            <end position="333"/>
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            <dbReference type="ARBA" id="ARBA00038125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON029474-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="333" mass="35355" checksum="B7DD8C0FA644CD06" modified="2020-08-12" version="1">MSLPGLPPLPKSLSGFDLAGMQFQSQHHHHQHHHHQQQQQQYQHQQHPAQHNPHYPPTHHQSAHHAHPMQQPSYHGTSPHPSSNLPPSSQSPALNHHHTNPFIPMVAGPAGLLHGGDSLASLESAQQRGHSPVSSTLSGGSSSGRKASPQPGVGPASASTLDTQLAILRREMYGLRQLDLSLLSQLWALNESIQEFRTMLQEQETLSPPSPSPSNSDANSVSSDDDLDEDDSSTTNNSTNRLLQQHQQQQQQQHHLLLSSAHHQLQSSTNSIVGAAGSGGSSGGGGGNLTPGGGPNPNESGSSSSTTLSSSASSRMRAPPPPPPNRKAPSRPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WA49</accession>
    <name>A0A6E8WA49_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Calponin-homology (CH) domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the smoothelin family.</text>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON029488-RA">
        <property type="protein sequence ID" value="ACON029488-PA"/>
        <property type="gene ID" value="ACON029488"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006858"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON029488"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032160"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd21200">
        <property type="entry name" value="CH_SMTN-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000009">
        <property type="entry name" value="smoothelin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050540">
        <property type="entry name" value="F-actin_Monoox_Mical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022189">
        <property type="entry name" value="SMTN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23167">
        <property type="entry name" value="CALPONIN HOMOLOGY DOMAIN-CONTAINING PROTEIN DDB_G0272472-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23167:SF88">
        <property type="entry name" value="CALPONIN-HOMOLOGY (CH) DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12510">
        <property type="entry name" value="Smoothelin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="5">
        <location>
            <begin position="2352"/>
            <end position="2458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="155"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="327"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="572"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="758"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="806"/>
            <end position="902"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="925"/>
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        </location>
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    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1025"/>
            <end position="1066"/>
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        <location>
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    <feature type="region of interest" description="Disordered" evidence="4">
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        <location>
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        <location>
            <begin position="1663"/>
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    </feature>
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        <location>
            <begin position="1821"/>
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        <location>
            <begin position="356"/>
            <end position="370"/>
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    </feature>
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        <location>
            <begin position="388"/>
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        </location>
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        <location>
            <begin position="444"/>
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            <begin position="466"/>
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        </location>
    </feature>
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        <location>
            <begin position="491"/>
            <end position="501"/>
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        <source>
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    </evidence>
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    <sequence length="2461" mass="265286" checksum="D769B53C30BF3FF2" modified="2020-08-12" version="1">MYKLREQRLKDFYASNDSSPPYFLMDNASATFGLKKSTVTGSHGDSLVDQSFESFKTKEIRDSESPTRFGSMALPADNSGWQIRTSEEMSEDGKTHTVRTAATTQGTKEIDGGRTAFAGQNLESHSEHFDGDGSNFVHSKGDQSSTVLVEDSVVEGEHGQRTVSGSTRSTTTSVSSTRSVVYQQQNGGDTASRPATDGVDRGTDRPLEFSAEHFSNATATAASSTESRTASEQQRDERAAPKELSKDPERVREAFRLAQGSGRIVEREETMVNTTTKMITETKQLDDGTTVTTRTYEKIAPPAEGVRASESSERDRFTSTAKVAVVQQEGVGRAVDRPTAAAASSSELIRQEKANQSSSSSSVTKTSTQQRIIVEVDAAHDSFARSLRSVSPTGSVRSMRSNATLTGRSSTSPEKVQPARPEPDTKPDYMRSTFTSERKVSGFQEEQQQQQQQQRSPSPRKMSGSVPASSRATSPTKGSSVTAGRGQEQPACDHQEVDGAKARGAPKPPLVRSETYEERCRKILGMPNRPAEEDGRTEAKSIFSTYDNTVTVQQSRSELREQRKKIEQEIKSMESKTMGALADERDSVVAEVSAGSRRMSIAKEPSPTRKMSAQHGSPTRKPSGTAKDPSSSELTKERSPSRKAPSRDISPIAKETPAPAKQPSKEVSPARKSPAKDASPARSTATPKDGSPVRGAPQPEQPAGRKEVSPARKEHSTETTRSKATILVEQKQEGRNISVAEITILPVLEVSHEAVRTKTTASRTHEKVLTKQSSDTKFDRRATSVTNSRVITNKSSSDFDIKRRVADLAAPDKRRAQPATTASPDKRRAAQPAQQKPNHITVAKIKIESPRKPTAGKPHPAEEPKPTGRQPHRAQAAEPETDPESSKDTGLSDNSEVEETVESVIEMSVTGTACCRHTDRKDSAPVYRTTGAARSGKSYTRSSSDNHLRATSASTHRHQPAVARDGSNVSAASATLKSAAGGRKVERPVKHVATKTINLATKPTAGSDSLDNVVIDIQLAKSSREPTPNKLVPIPVSPDTEDAGKPRYPDAVQEPDDEAAGGRPARNQRVSTIPIFEEATSEYVGCEITEVDEQQEASAAQQATRITNLDRVTEDDESLLSVTEKVNKFAQEARRLQADTNGESQRPPSMGRFAARPEYDDLDDHLKSDECLLSVSDKVTKFISTAEEVKKIRTSGPFVPGTDDGPVKVADGDECLLSVNEKVSRFASRMTRAVDARDDAEEDESDQQQRTAVPGGLGESVPGKFVPQRSPELVKNAMRTTARHLVEEHAPGDELAQSVGSIASRYRTATMAKPYEKAPAGGAPAASPITLRSTEAVKKAKELFEKGQTDGGRQRDILNRPSVWEERRTRAQPAAEPKRADVKLTDIGVTERKAQTVREEHVVGEESAPRKPSITKVEQQRPAEPAEAAKPTGSAGGRRDSSGSGVRPPAYIRDVVSTKKDLFEKRISSSKMQVEYTSQTSQEATETQATATLRKQSLKQAGEAVPSPSTPANRPSADQSKPSYMNHTVASLEHINANQRRDSLDHGPAAHHETTTTSTMMTTATTTTASARSTTTTGSAKFGVELKRLDTNGRMVATNAPAVSSAGSKRKPSTGEAQPTAGSSPAIEEIFDLELLERMLESVTGYEQRRRIRAQIRVVKKQKEQQQQQTSAGSQTQTKQTSVTTSKSVTTTRDTAAGKRKDSSPTRASVVLGGRKQSEPADATDAAAPKRQASIEAEGGRKATANLTAKTMTTVTTERFASKAGTADRVPPTTKADDRPIWATSNILKKASENTRTFKSVTTSASSAGCATGGRKVVSTSSSYHQQQQQTKTATQDPKTTTDCITSSYGVGPTDENGLPLFGIRALKKKAAPAPADDTATTTKISGTVITETLVAENGGPAVGKRSTTHYTSEPSAFGDADGVGKLTAVRKTETIDAAGTHAEEFEMLDGLELAGRREAKVVRKGSVKELTERFIHRESSGSLTQDATRTYPKAGLILRSSAQQSQSSRASTPATDGCSVRSGSVDGYDQEEEEAHSMTTMTTRQVRSFLNDTSKVVDVRDVLQRMSNADNVTEQGDSAEDQEARALLNKFLGASVLMSGVESMVSSPGTMLGEASAPSASGGTKKVSLVSKVSSTSTTTKTVKSSGVTPSAAGSKQATVDTLEDNWDEAVLKQLLESTTNYDDRRKIRARIRQVMAEKEACADIVAIVTADLQRERHLQQQQPSGTMGAASAGSGGAADRQTAATAAAANGLSQVQQTTTRTIANTKMESQDGSAAVTTRTTVTTKTVASSAPKPMSPFAKFRQLDKQNSLSSQSPPNSPKTPSTPTTPSGAMGGPMFKFTDPALNRRAATVKDQLLQWCQMKTKEYENVKIENFSTSWSDGMAFCALIHHFLPDAFDFSKLTPQQRRHNFTLAFRVADEKAGIAPLLDVDDMVAMRKPDWKCVFTYVQSIYRRFKNEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WA52</accession>
    <name>A0A6E8WA52_ANOCL</name>
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        <submittedName>
            <fullName evidence="3">Fibronectin type-III domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
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        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
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        <property type="gene ID" value="ACON013210"/>
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    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039820"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="169"/>
            <end position="193"/>
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    <feature type="domain" description="Fibronectin type-III" evidence="2">
        <location>
            <begin position="48"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
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    </evidence>
    <sequence length="210" mass="24484" checksum="32B4F1E7ADC55791" modified="2020-08-12" version="1">MFAARSILCRLYNTLIFMHLIYVTRSFGVIFEEYVVDQGRNLTLRCDSKHPVRWVREGRREDVRQFQPNRTGGYAIRDFTAEMRKMRESSNETELPWETIDPRHISPNARQLEIYHLIPNTTYEFRIWGNNQLGAGEIVTILATTQPRMEEKDLVRRIMIDAKNFDTRVWIAAVGIVMGTLVILSLGTCIVLYKECREPAGSTNDFPHRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WA81</accession>
    <name>A0A6E8WA81_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="9">C2HC/C3H-type domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
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                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ZC2HC1 family.</text>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON028496-RB">
        <property type="protein sequence ID" value="ACON028496-PB"/>
        <property type="gene ID" value="ACON028496"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002326"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON028496"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042635"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049899">
        <property type="entry name" value="Znf_C2HC_C3H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026104">
        <property type="entry name" value="ZNF_C2HC_dom_1C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14649">
        <property type="entry name" value="ZINC FINGER C2HC DOMAIN-CONTAINING PROTEIN 1C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14649:SF1">
        <property type="entry name" value="ZINC FINGER C2HC DOMAIN-CONTAINING PROTEIN 1C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13913">
        <property type="entry name" value="zf-C2HC_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52027">
        <property type="entry name" value="ZF_C2HC_C3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2HC/C3H-type" evidence="8">
        <location>
            <begin position="84"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="166"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="320"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="483"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="666"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="808"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="872"/>
            <end position="985"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1059"/>
            <end position="1120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1163"/>
            <end position="1226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="171"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="323"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="339"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="490"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="685"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="702"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="737"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="872"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="886"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="929"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1059"/>
            <end position="1110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1170"/>
            <end position="1188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1204"/>
            <end position="1218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS52027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON028496-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1294" mass="142622" checksum="BE1450C2875E7335" modified="2020-08-12" version="1">MDSDTDFERTINNAMDALENAIENGFDEDFKERYDHRFEGSWSDNNLNEKQLYTEAEKEEPVYIEECKSYETFDPSYEPPPNLELQPCPICLRKFAPASLSKHTGICERVQTKKRKPFDSSRQRREGTELASYLPKNFGLPQKTVSSSNEAIPVRKLSLSKTPTFERKEFSPTTMSTSMPSASTPKPALKRSMSQQNEPCPYCERCFGMKAYDRHVEWCREKAILNRNVNNNQSISAAKERLQARIQYKAPQIRSKRALNREKYSGSLSCSGSTNSLAELDLHMPRHNSMSSSVSSDNGYSPDRYDPFLSARRQLEELFSPATSLIHTSSPSSTTSTSRLNQMSPNGGTANDKSASGNATKHTPHNSNFRRAYSLRMPRKVSRPMYVEKAKSNIQKGITDDGPVSPNFLKSSEYDELPIKSTFNALQMAEPKPKLRDSSTVRKNLKLEIRDPNNPAGGEIPLSKTDSLAVFLKYENELALSEKDMKDKSNSLSKRTSSLSAEVNQQKKQEVLSVAPSPVKANETITVDEEQQKHKKNVQAIEDETAKKQTQKLVPIKLEPINITYNSNNASEVTDIKPVVISLDAIIGKPSIKKQKEPQVDSDNRADNSYIDPKLINKCDNLPINLVMPSTKDADGSCSSRNSNTIGNICSDGNQLALKTIEPKVERSAVISPPPRASLSVAPKPPERTRNETCFDYRKSNNEPSSSEPSTSNNSSPTTSHSSSPVDHRISKNSTNCDKRPQLNRQDREDSGYRTGQESGQSDVHEQPKKPSPSPSASKNNEDNMPKNPAFKPTYKLNRLISPSSALNNFSNTLPSSNSTAPSTPVNRDRPTISSALEQFDVDEFMQSLEDLHRQSPISAREYKHSLFVHSNSSVTSRSVASSQSDHTNNNKRSNSLDSGHGNSVANSIRYVRKHESSGVPRMDVCVRNGSGTASNNNSSNSSSSFSSPSHYSNSNNSNSYYNSSSTNNNNSHNHSTSASNANDAAKCAEVVHPLGQQMNYARNSPAIAHRRRSEDAAGISLPAVPPLPSSNTGGSGALSSLQNLPNIHKATAADTLLNGNNKTMQQPGTLPYYSSPSPVNGRKNSYSNKSYHANNTGTSASTPASSHLTGNVHEPRYPAESFDHLERDLLKSVQELNRMCGSSSSVCSIDSEELYSVEDYPLNKRSSERSQASADSAYRSSLSTQSPPDYAPPVPIRQARPNSRSSTTSQSQPAQSTNEPFNSLSLHTALPPITTAIIKGHRMEAHLMKDSPEGGPNLIMNPMSKFCHECGARFMISSAKFCMSCGVRRIMLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WBU8</accession>
    <name>A0A6E8WBU8_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_040235260.2">
        <property type="nucleotide sequence ID" value="XM_040379326.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040235263.1">
        <property type="nucleotide sequence ID" value="XM_040379329.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040235264.1">
        <property type="nucleotide sequence ID" value="XM_040379330.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040235265.1">
        <property type="nucleotide sequence ID" value="XM_040379331.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040235266.1">
        <property type="nucleotide sequence ID" value="XM_040379332.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8WBU8"/>
    <dbReference type="EnsemblMetazoa" id="ACON028188-RA">
        <property type="protein sequence ID" value="ACON028188-PA"/>
        <property type="gene ID" value="ACON028188"/>
    </dbReference>
    <dbReference type="GeneID" id="120957239"/>
    <dbReference type="KEGG" id="acoz:120957239"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007388"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON028188"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034809"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON028188-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="206" mass="22235" checksum="5CAC1414DC57799D" modified="2020-08-12" version="1">MSLEETRRTQRPYRYGMMLLCVGALVNWLGLAENYSEPVRYAGVACILAGACLICTAMCCWLHTPGRSGTNGDEGDDPVHVISANDERRREKPPDYDTVAAAPPSYDDAIKLDPAALLHLSIASNANVSSNANINYIPTVHPSSSHTMSNSNCTETVQSTEGSQILDCSITVCDEKMCSEKPPPYAEPVVCGTSISSDERTSRSTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WC72</accession>
    <name>A0A6E8WC72_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ankyrin repeat domain-containing protein 12</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8WC72"/>
    <dbReference type="EnsemblMetazoa" id="ACON029520-RB">
        <property type="protein sequence ID" value="ACON029520-PB"/>
        <property type="gene ID" value="ACON029520"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004961"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON029520"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040397"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053210">
        <property type="entry name" value="ANKRD12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24149:SF14">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24149">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="15"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="85"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="156"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="200"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="242"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="256"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="275"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="352"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="362"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="378"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="395"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="439"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="474"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="520"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="531"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="560"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="643"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="670"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="682"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="721"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="771"/>
            <end position="788"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON029520-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1015" mass="101443" checksum="A58F5E29BF714165" modified="2023-06-28" version="2">MQRRGGLVAPAAVLSHQQQQPPSAQPHHLGRSEPGATGPVSASQSKDNAVSSRHHGPHMKKSANSSADTGSSGGGPSSGKRTHGNSNSNSSNSNTMGNTHQDNDHAGEHAGLGGSASTKDKDEDDIYEFKSTPKDSSSSSSDEKEVSGGGGGSKKGSGDKTGDGAHHHDAHDHHHGGSSRDDPLALGHHHAADDKQKDGSGSGGADHGPGSGGSLSSTSGVPKRPFSELNDGGDDGGPTNEDETKRKKRKDADPLGGLGGVGSGAGKDTASSGSLSGGKSGTGGSRGSTPRQEKGGKAAGGVVSAKTLGLSAKGALDRKSPCASPKPSQGGVGAGGGSSMASGAAAGTNVTGSGGSAGSIGGTAASGSTAAGKGGSSGTSSSNDSDGESTLMGGAEDGGNGKGGGEGGFGGGFSGPKVPPLKIVIPQQSAAGDAEVGGNTRNGKNASTRNHAALPYVVASSNSNDSTADKESTSSRSASPADSKSSDEKNPSQSGTSKDERTQQRVLRSSHRGGNSNSSAKDGNTSGVDRSNNSSPQMQQQGNSGSPSPISSGGCDATERGGGGGTSGGASEGGGRSTQTNSPAGATATGEGDNGSSTGGTGTGGNPKSPTNVTGATGGTGTGNSGSNDSSAGTGTGGGTNGTGSSSNSTARGDGSGGGASGAASSGGQSSAGGNGTGSSGTGATQNNNSSSSSSSSSSSSTSESSVHPRKRKIKTNKDQSAATASSGAAAGSNATGTGSNSGTAPAGSAGSSASSSSSSSSTSGAGATASEDKKEAREETPDVHPHDQPITNCYQMYLNIRKQIERRQKSLFPVQPKPPQGFKDYLMNRCTYALAGKTPTEPNVQVPATLPPSLKEMFQAQEKERHKLRMQHIVEKEKLVLAVEQEILRVHGRAARALANQSLPFSVCTILKDEEVYNIITPEQEEKDRNARSRYNGRLFLSWLQDVDDKWEKIKEAMLLRHHNEAESLHAVQKMDWEWKMKEHLLCEFKAKPFIDETYVPMVNVNDDFDLLPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WC78</accession>
    <name>A0A6E8WC78_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_040218721.2">
        <property type="nucleotide sequence ID" value="XM_040362787.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8WC78"/>
    <dbReference type="EnsemblMetazoa" id="ACON029526-RB">
        <property type="protein sequence ID" value="ACON029526-PB"/>
        <property type="gene ID" value="ACON029526"/>
    </dbReference>
    <dbReference type="GeneID" id="120947455"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_013092"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON029526"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042579"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="76"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="43"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="384"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="424"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="471"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="131"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="385"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON029526-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="523" mass="57055" checksum="34BC2FF57C1CFF79" modified="2020-08-12" version="1">MNSNTIKMKGDQKNESFKLLQLSDDSKSLSDLELAEVVSQRKRKVRRKTRQRTSSYRGDEERSLSGRSGQIDCKNFSIWLAVAMTVLWLFIISYVTSVIHGENHRLELALQKVSATSQNVPEALQKWHETSKNLEQNQTALNGKLRDMEQVLTNFFTELKQLRETIDKKNENSQEAQLNRLQSSVADLGSNIGDANSRIGLLETRFDTIQAEQKQLNKTLDDLQKLFGRIQNSTAVSDIIGGDGVAKGMQKTIAELRDQLTGQLNNLAQNVTGELQVLKQKNLWLESDLSNQTKRIEQLFDNTVNISSHVLSVEAVWLEVRNNISGLEADRKIINEQLGALANVTTGLHGTIEKVQEECQQYHSKLDEVRGKLGELQDQIQQNAARKEVSLHRPGEANGTQQASKEDGMPPVLSQFFNEQQTAASSSSTVSARIAPKPTAAPSSLSASTTTITTASSLAKLLYPGTLVQQLPQSTTPQPSAAGQPASAAKALAAGAPASGAANTASSSSSSSKQAGGLFDSVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WC82</accession>
    <name>A0A6E8WC82_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Phorbol-ester/DAG-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8WC82"/>
    <dbReference type="EnsemblMetazoa" id="ACON029530-RB">
        <property type="protein sequence ID" value="ACON029530-PB"/>
        <property type="gene ID" value="ACON029530"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_013033"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON029530"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030565"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903078">
        <property type="term" value="P:positive regulation of protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003009">
        <property type="term" value="P:skeletal muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20817">
        <property type="entry name" value="C1_Stac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000056">
        <property type="entry name" value="Uncharacterized protein, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000071">
        <property type="entry name" value="Uncharacterized protein, isoform U"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039688">
        <property type="entry name" value="STAC1/2/3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15135">
        <property type="entry name" value="STAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15135:SF7">
        <property type="entry name" value="STAC-LIKE, ISOFORM J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="12">
        <location>
            <begin position="493"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="364"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON029530-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="712" mass="78749" checksum="894F6F59C01E3BED" modified="2020-08-12" version="1">MSSPTLRRPRKVTTESSKSHRHNIFSKRGATPSAGPAFQSLGGSMSTSGGGGVLKDLGHPGLNEALKSHNGVTFKLVKTVSDFSETLAQLYEQHAEALQVLVSNYRKRNSELRKERPACQSNLFAAWETLLQEIEADSQATIDVASTLSRQVARPLLERSFYRKVQSRKVFTHRESFDTIISKTEEKLSKCRIEYKQCYIAHRQSPTQHTLTQYIDSHNAYVQQLHATNAMLEAYHCETLPQLMQELEEIYNDLCNIVSEAVLQGAEAIAAKAAEQAKRYDGLAVQCKNVSPALDLGYFVRTLPVPQNNQRIPRKAFAPPQSANAPTDAGADDYPDYGGDPMLPVFKDELVVDRGATIQVRPSLEALRRESQELEIQIKQLQDSVDALVRTQIRGIEGQLYSKANEIQEDISMKKFDVRAKQIHLAAVRAQKELFLGRIDPGSPRGERKMSSSSSSSMKTKWLKAFRSLKPAGSANDRKSGNGAQIRFDGTDNHNLQEYTYKKITPCDVCSQVLRGHTRQGLRCRICKVNVHADCASQLPKCQVKQKLLRRQKSTSEIENRATEVEEEKPTDVDQIYQVLKQAGEISSGKIVVDPSKGPGPIGSVAAAAAAAAAAAGHNQTVSNPPLDIPVVKVPEYPDRAPWRGPVKSQGPNYPHQKQGLTAPVATDMSCSAEEDDIMISTRPFGQPLCFVVEEPPPEEMQSGKKDGKRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WDZ5</accession>
    <name>A0A6E8WDZ5_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Phosphotransferase</fullName>
            <ecNumber evidence="14">2.7.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes the phosphorylation of various hexoses to hexose 6-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>D-fructose + ATP = D-fructose 6-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16125"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37721"/>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:16126"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>D-glucose + ATP = D-glucose 6-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17825"/>
            <dbReference type="ChEBI" id="CHEBI:4167"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61548"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:17826"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>D-mannose + ATP = D-mannose 6-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11028"/>
            <dbReference type="ChEBI" id="CHEBI:4208"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58735"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:11029"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a D-hexose + ATP = a D-hexose 6-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22740"/>
            <dbReference type="ChEBI" id="CHEBI:4194"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:229467"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:22741"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate metabolism; hexose metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the hexokinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.-" evidence="14"/>
    <dbReference type="RefSeq" id="XP_040239949.1">
        <property type="nucleotide sequence ID" value="XM_040384015.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8WDZ5"/>
    <dbReference type="EnsemblMetazoa" id="ACON029633-RA">
        <property type="protein sequence ID" value="ACON029633-PA"/>
        <property type="gene ID" value="ACON029633"/>
    </dbReference>
    <dbReference type="GeneID" id="120960075"/>
    <dbReference type="CTD" id="45875"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002473"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON029633"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031770"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00180"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00242"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005536">
        <property type="term" value="F:D-glucose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008865">
        <property type="term" value="F:fructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004340">
        <property type="term" value="F:glucokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001678">
        <property type="term" value="P:intracellular glucose homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd24019">
        <property type="entry name" value="ASKHA_NBD_HK_meta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000095">
        <property type="entry name" value="Phosphotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.367.20:FF:000005">
        <property type="entry name" value="Phosphotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.367.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001312">
        <property type="entry name" value="Hexokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019807">
        <property type="entry name" value="Hexokinase_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022673">
        <property type="entry name" value="Hexokinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022672">
        <property type="entry name" value="Hexokinase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19443">
        <property type="entry name" value="HEXOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19443:SF16">
        <property type="entry name" value="HEXOKINASE TYPE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00349">
        <property type="entry name" value="Hexokinase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03727">
        <property type="entry name" value="Hexokinase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00475">
        <property type="entry name" value="HEXOKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00378">
        <property type="entry name" value="HEXOKINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51748">
        <property type="entry name" value="HEXOKINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 14" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="6 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Hexokinase N-terminal" evidence="15">
        <location>
            <begin position="10"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="Hexokinase C-terminal" evidence="16">
        <location>
            <begin position="211"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00050361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00059457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU362007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF03727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON029633-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="454" mass="50704" checksum="58CC817F4537A49A" modified="2020-08-12" version="1">MSEMEVLEEIREQCKELILTDKQIEEIMRRVIKEINRGLSKETHAEADVKCFITYVQDLPNGKEKGKFLALDLGGTNFRVLLIHLKDENDFEMLSKIYAIPQSIMLGSGTQLFDHIAECLANFMKEHSVYEERLPLGFTFSFPLTQLGLTKGILARWTKGFNCSGVVGEDVVQLLKDAIARRGDVQIDICAILNDTTGTLMSCAWKNHNCRIGLIVGTGSNACYVERVENCDLFDGPKSGPNIKQHVMINTEWGAFGDDGALDFVRTEYDREIDQHSINPGRQLQEKMISGMYMGELARLAIVRFTKAGLLFGGVGSDILFKRGQFFTKYVSEIESDKPGTYYNCREVLEELGLEHATDEDCANVRYICECVSSRAAHLVSAGIAALINKMDEPSVTVGVDGSVYRFHPKFHDLMVQKIRQFVKPHISFDLMLSEDGSGRGAALVAAVACREAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8W7PJL6</accession>
    <name>A0A8W7PJL6_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">S-adenosylmethionine decarboxylase proenzyme</fullName>
            <ecNumber evidence="13">4.1.1.50</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="19">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2022-08" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12 13">
            <text>S-adenosyl-L-methionine + H(+) = S-adenosyl 3-(methylsulfanyl)propylamine + CO2</text>
            <dbReference type="Rhea" id="RHEA:15981"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57443"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="4.1.1.50"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </cofactor>
        <text evidence="13">Binds 1 pyruvoyl group covalently per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 13">Amine and polyamine biosynthesis; S-adenosylmethioninamine biosynthesis; S-adenosylmethioninamine from S-adenosyl-L-methionine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the eukaryotic AdoMetDC family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.50" evidence="13"/>
    <dbReference type="RefSeq" id="XP_040232356.1">
        <property type="nucleotide sequence ID" value="XM_040376422.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8W7PJL6"/>
    <dbReference type="EnsemblMetazoa" id="ACOM032611-RA">
        <property type="protein sequence ID" value="ACOM032611-PA.1"/>
        <property type="gene ID" value="ACOM032611"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON009619-RB">
        <property type="protein sequence ID" value="ACON009619-PB"/>
        <property type="gene ID" value="ACON009619"/>
    </dbReference>
    <dbReference type="GeneID" id="120955506"/>
    <dbReference type="CTD" id="34396"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037684"/>
    <dbReference type="Proteomes" id="UP000075882">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004014">
        <property type="term" value="F:adenosylmethionine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008295">
        <property type="term" value="P:spermidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006597">
        <property type="term" value="P:spermine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.90.10">
        <property type="entry name" value="S-adenosylmethionine decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048283">
        <property type="entry name" value="AdoMetDC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001985">
        <property type="entry name" value="S-AdoMet_decarboxylase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016067">
        <property type="entry name" value="S-AdoMet_deCO2ase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018166">
        <property type="entry name" value="S-AdoMet_deCO2ase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11570">
        <property type="entry name" value="S-ADENOSYLMETHIONINE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11570:SF0">
        <property type="entry name" value="S-ADENOSYLMETHIONINE DECARBOXYLASE PROENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01536">
        <property type="entry name" value="SAM_decarbox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001355">
        <property type="entry name" value="S-AdenosylMet_decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56276">
        <property type="entry name" value="S-adenosylmethionine decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01336">
        <property type="entry name" value="ADOMETDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 17" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="4 13" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="9 13" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 13" id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword evidence="11 13" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="10 13" id="KW-0704">Schiff base</keyword>
    <keyword evidence="6 13" id="KW-0745">Spermidine biosynthesis</keyword>
    <keyword evidence="8 13" id="KW-0865">Zymogen</keyword>
    <feature type="chain" id="PRO_5044537269" description="S-adenosylmethionine decarboxylase beta chain" evidence="18">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044537268" description="S-adenosylmethionine decarboxylase alpha chain" evidence="18">
        <location>
            <begin position="81"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate; via pyruvic acid" evidence="14">
        <location>
            <position position="81"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor; for catalytic activity" evidence="14">
        <location>
            <position position="95"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for processing activity" evidence="14">
        <location>
            <position position="285"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for processing activity" evidence="14">
        <location>
            <position position="299"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="279"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="303"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Cleavage (non-hydrolytic); by autolysis" evidence="17">
        <location>
            <begin position="80"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="modified residue" description="Pyruvic acid (Ser); by autocatalysis" evidence="16">
        <location>
            <position position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR001355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR001355-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR001355-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR001355-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR001355-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR001355-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACOM032611-PA.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009619-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="389" mass="44680" checksum="5CE1B92B9D8DBC3F" modified="2023-06-28" version="1">MAESEDLSNSSSNSSSREDMHFFEGVEKLLEIWFEPSTACKNADLRKIPRPMWDALLKTVRCEIISFTRNDQIDAYVLSESSMFVSKRRWILKTCGTTTPLQCFEPLLRLAAEIAGYTEIEDLFYSRKNYKRPELQVSPHRGFDEEVAFLDSFFDDGRAYSLGAINRDCWHLYTLSRGGGGSKILKRNNHLHQLMEQHHSQQLQQQQQEDDDVQQLDAQLIEASMQPDPDQTIEILMTDLDPSVMAIFTKDVCTTAKEATQKSGIHRLLPGMVIDDYLFDPCGYSMNGISKNGCYMTIHITPEREFSYVSFESNVASSDYGELIKRVTRTFQPGKFIVTVFANKTSIAANANREIEHLGTIDQWKRRDIQYSRFASYDLTYAQYCKYPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9I3A8V5</accession>
    <name>A0A9I3A8V5_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Ionotropic glutamate receptor C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2023-03" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="23">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Postsynaptic cell membrane</location>
            <topology evidence="14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9I3A8V5"/>
    <dbReference type="EnsemblMetazoa" id="ACON000798-RB">
        <property type="protein sequence ID" value="ACON000798-PB"/>
        <property type="gene ID" value="ACON000798"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032407"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015276">
        <property type="term" value="F:ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
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</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9I3A8W1</accession>
    <name>A0A9I3A8W1_ANOCL</name>
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        <recommendedName>
            <fullName evidence="25">Ionotropic glutamate receptor C-terminal domain-containing protein</fullName>
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        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
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        <citation type="submission" date="2023-03" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="23">Ngousso</strain>
        </source>
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        <subcellularLocation>
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            <topology evidence="14">Multi-pass membrane protein</topology>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="InterPro"/>
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        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
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            <begin position="27"/>
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            <begin position="830"/>
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        <location>
            <begin position="434"/>
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            <begin position="901"/>
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            <begin position="934"/>
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        <location>
            <position position="520"/>
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        <ligand>
            <name>L-glutamate</name>
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            <position position="525"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="692"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="693"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="740"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="site" description="Crucial to convey clamshell closure to channel opening" evidence="16">
        <location>
            <position position="670"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with the cone snail toxin Con-ikot-ikot" evidence="16">
        <location>
            <position position="698"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="752"/>
            <end position="810"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="SMART" id="SM00079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="SMART" id="SM00918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000798-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="25">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="944" mass="105423" checksum="D594F64EFF626FBA" modified="2023-06-28" version="1">MSTIGRRRWKVLFALLLITQLAAVTGYHHGDLDETVEHIKIGGLFDGATEDVELAFQYAVEAVNNEKLSYSNYQLEAQAVQVKYGDQFDASKKLCRLLKTGVAGIFGPSSPKSALHVQSVCDEKEMPHIETRWDAYTKLPTLNLHPHPHIMGRVFLDLVVAFEWKSFTVLYESGPWLPGIADLLKMYDPKGYTVTVRQLDLGLNGNYRAVLRRVKLSEDKRIILACSIDSMPEVLRQAQQVGLLTDHHQIIVTSLDLHTIDLEPYQYSGTNITGVRLIDPEEGKIRQVADFLNASQIAKTLELQDGLNPAKMRVETALMYDAVLLFAEALKHLIGSEPAHLLEPIALRCDDTATWKNGYSVINYMKSSTIHGLTRSIRFDHQGHRSDFLLDIIELGPAGLEKVGVWNSTEGLNFTRKKEQTLLAFDDGTLQNRTFLVLTAISPPYGMLKDSPVKLTGNERFEGFGIDLIHELSLMLGFNYTFILQEDGVYGSLNRDTKKWNGMVLELLEWRADLAITDLTITSDRESAVDFTMPFMNLGISILYRKPTKEPPSLFSFMSPFSKQVWLYLGGAYMMVSMSLFILGRISPKEWDNPYPCIEEPEELENQFSFSNSMWFTIGALLQQGSEIAPKAPATRAVASIWWFFTLIMVSSYTANLAAFLTVEQVVSPISNAEDLAAAGGTIKYGAKRDGSTISFFKDAEYGTYAKMYQYMMANQELLTSSNPEGLQRVKTENYAFLMESTSIEYIVERECDVTQIGGLLDDKGYGIAMRKNSPYRSALSEAVLRLQEQGVLTSLKRKWWKEKRGGGACSNTMEEGGALALELSNVGGVFVLLIVGCVAALFVSFCEMLCEVHSRTRELKELMAELRFVAKCHGNTKPVRHRKSSSASGENSLELDVEDSTSSSKQDISGNGMGDRLPTGNQRRQNGGKLALNGNTRKMLSED</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9I3ANA2</accession>
    <name>A0A9I3ANA2_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="10">SPX domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2023-03" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SYG1 (TC 2.A.94) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040228927.1">
        <property type="nucleotide sequence ID" value="XM_040372993.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9I3ANA2"/>
    <dbReference type="EnsemblMetazoa" id="ACON005557-RB">
        <property type="protein sequence ID" value="ACON005557-PB"/>
        <property type="gene ID" value="ACON005557"/>
    </dbReference>
    <dbReference type="GeneID" id="120953226"/>
    <dbReference type="CTD" id="38666"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030840"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000822">
        <property type="term" value="F:inositol hexakisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016036">
        <property type="term" value="P:cellular response to phosphate starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14477">
        <property type="entry name" value="SPX_XPR1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004342">
        <property type="entry name" value="EXS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004331">
        <property type="entry name" value="SPX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10783:SF103">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 53 MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10783">
        <property type="entry name" value="XENOTROPIC AND POLYTROPIC RETROVIRUS RECEPTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03124">
        <property type="entry name" value="EXS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03105">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51380">
        <property type="entry name" value="EXS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51382">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="234"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="268"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="338"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="499"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="domain" description="SPX" evidence="9">
        <location>
            <begin position="1"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="EXS" evidence="8">
        <location>
            <begin position="427"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="70"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005557-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="676" mass="79762" checksum="BFF35C64AE6DCB03" modified="2023-06-28" version="1">MKFAEHLAAHITPEWRKQYIQYEEMKAQLYAAVEQSPSAELVDPEVLTRYFAKFDEQFFHYCDSELAKINTFYSEKLAEATRKFANLRTELSETLEMEESTKMKKKDNLHKMKKNLLRKKNVSVRKIQELKLAFSEFYLSLILLQNYQNLNFTGFRKILKKHDKLLNVDFGARWRAEHVESAHFYVNKDIDRLIHETENIVTNEIEGGDRQRAMKRLRVPPLGEQQSPWTTFKVGLFSGSLIVLCVAVVLSAMFRLRRDDWIVAFRLFRGPLLIVEFMFLWGINVYGWRSSGVNHVLIFELDPRNHLSEQHIMELASIFGVIWTMSVLGYLYADALAIPAYLSPLILYLLMAGFLLNPTKTFRHEARFWTLRILSRIVLAPFFYVNFADFWLADQLNSIVPAFLDLQYFLCFFSTISNWSYAEDPNQCINNSLWIRPVVAMLPAWFRMAQCLRRFRDTRDAHPHLANALKYSTSFFVVIFSSITQATRDQYAKSSDNPWFYLWILASIVSSCYAYTWDIKMDWGLFDSKSSDNKFLRDEVVYSSNWFYYFAIVEDLILRFGWTLSMSLIEMGYIDREIIVSILSPLEVFRRFIWNYFRLENEHLNNCGNFRAVRDISVAPMDCSDQTTILRMMDEEDGVRNRRTVKKLANKKKSEQRLLLREAESTEDLEILHRIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9I3AZ39</accession>
    <name>A0A9I3AZ39_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="22">VWFA domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2023-03" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="22">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_040232465.1">
        <property type="nucleotide sequence ID" value="XM_040376531.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9I3AZ39"/>
    <dbReference type="EnsemblMetazoa" id="ACON009598-RB">
        <property type="protein sequence ID" value="ACON009598-PB"/>
        <property type="gene ID" value="ACON009598"/>
    </dbReference>
    <dbReference type="GeneID" id="120955553"/>
    <dbReference type="CTD" id="34921"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042294"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005891">
        <property type="term" value="C:voltage-gated calcium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005245">
        <property type="term" value="F:voltage-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12912">
        <property type="entry name" value="PDC2_MCP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000007">
        <property type="entry name" value="Calcium voltage-gated channel auxiliary subunit alpha2delta 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000057">
        <property type="entry name" value="Voltage-dependent calcium channel subunit alpha-2/delta-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051173">
        <property type="entry name" value="Ca_channel_alpha-2/delta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033479">
        <property type="entry name" value="dCache_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013680">
        <property type="entry name" value="VDCC_a2/dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013608">
        <property type="entry name" value="VWA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10166:SF37">
        <property type="entry name" value="STOLID, ISOFORM H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10166">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL SUBUNIT ALPHA-2/DELTA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02743">
        <property type="entry name" value="dCache_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08473">
        <property type="entry name" value="VGCC_alpha2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08399">
        <property type="entry name" value="VWA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="5" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="17" id="KW-0407">Ion channel</keyword>
    <keyword evidence="13" id="KW-0406">Ion transport</keyword>
    <keyword evidence="14 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 20" id="KW-0732">Signal</keyword>
    <keyword evidence="7 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="11" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039918779" evidence="20">
        <location>
            <begin position="40"/>
            <end position="1256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1235"/>
            <end position="1254"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="21">
        <location>
            <begin position="269"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="701"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="746"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1058"/>
            <end position="1114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="1059"/>
            <end position="1075"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="1093"/>
            <end position="1104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009598-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1256" mass="141890" checksum="7B9EEC86885D7FA2" modified="2023-06-28" version="1">MEPLPQSRWWRWRRRHLLTGSLLLLVCLLLAIDPTPVSADPDEDIPHNEVRNWAIRFGVDLWEFGRQFTKVNDIRNRFKDSDVDVTRKDGILLLRELAAEVKNFMDFKMNAVMRIMDSAEQAALSESDPESATSKAHPSAFYDARRINEYQSDGRLAEGSRQMLLRHMRRFEGYPVNISLSSVLLPAGVSLDDPETQSAIKWSSHLDPLFANNIERDSALSWQYFGSSTGFLRRFPGTAWPPETSYGSKEINDFRSEDWFIQAASSPKDVIILLDSSGSMSGKEYQLAVATASAILDTLGDDDFFNLISFSDQSRVIVPCFQDKMVRATPDNVKEVKTAINAVECENTANFSAALETAFELLRKYNQSSQGSQCNQAIMLITDGPSDTFMEVIKHYNHPHMPVRIFTYLIGTDKSGGKNLYKMACENKGFFVQINSPEEAKKKVVEYALVMARPMVLYQADHPVHWSPVFMGGRSGILGRESENRRKLVTTVSTPVFDRRNHSTRAANLLGVVGTDVPIEEIQKMIPQHKLGVNGYAFIVDNNGRVLYHPDLRPLSDNDQYSATLKHKYNSVDLTEVELPEVDNPSNTINERHDQRYANTLQELRNEMVMQKEGENELTVLTHLDSMKRVSLRFQKYFYGAIDGTPFSLGIALPDSYGVHELNAQQEIRHSHINVTEHFKGNNWKVHPDWVYCEYNSLKDADGNGEGTEESTYRDKDESFDTPEEQVLHFLARVGRPGWKWMSVRPRSPQPHHGHGGIPVGHYAQHHFNSQGSRKAEPYYCDRTLVQSLVRDAIVTDGLDRTPSHPSRKEDRSRLGFSMFSVKTTFVATRSGLLRWIDHLPHSEDSSEPHFSETNARAMDMSWYKRAVDLYATEPEGFVFSVPFNSGYSGKNSSTLVTASHAIFIDHRGHKAPAAVVGLQFLHESLFKHFINITSKCTASTTCKKNCASDELDCYLLDDNGFVILSERSEHTGKFFGQIDGTIMDSLVQDRIYRRVPLMDYQGICSDRDNPYTGAGEPLKPVRPMSWLLQYFVSFATYWLSVLPTPIGAWQNSNYNYDGTDDIDDEDTYDYEQPDYELPPEHSDVTTPDYDQRSTPTPQRSHTQAGPRVVPDPAHARPCDLKTDLYVLQPERLNSSGQNNPLKGKLTNCHSSGCERPFSVQKIPNSNLILLVVDVLCPCGSKQLDIDPQEVVGGAGACGVRRMAKEKMLRKRPGKCISYHPEEIEIKQCGTATSLFHASLYSTIATFIVIWVLASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9I3BDJ3</accession>
    <name>A0A9I3BDJ3_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2023-03" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9I3BDJ3"/>
    <dbReference type="EnsemblMetazoa" id="ACON030723-RA">
        <property type="protein sequence ID" value="ACON030723-PA"/>
        <property type="gene ID" value="ACON030723"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031321"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000249">
        <property type="entry name" value="facilitated trehalose transporter Tret1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050549">
        <property type="entry name" value="MFS_Trehalose_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48021">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48021:SF89">
        <property type="entry name" value="FI02132P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="41"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="84"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="111"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="143"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="176"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="202"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="294"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="330"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="362"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="389"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="424"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="457"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="43"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON030723-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="509" mass="55316" checksum="F10E8DF13A026454" modified="2023-06-28" version="1">MDKARETPSNMPADPSANKAAEASASSGIAKLTKRAAITQIIAAVVANITIISSGMGIGFPSIAMMELTNSTTSVVLTESEASWFASITSIMCPFGGLLSGYLLDKVGRKRTLYCINIISVISWAIMSFASRTDASVLFVELIVARVIIGIAIGLSSTPASVYAAEVAHPRLRGRLTLLTAGCTGIGMLLVYSLGYMFKEDWRYVCTICGVFTGVSLITVIPLPESPSWLVGKRRPQDAERYLKVIRAIKEDNHPEIKAEIKSLEENVARFRSSETKKSSKLVQLKKPEVYKPLSIMCIFFLFQQFSGIFVIIVYAASFSVEAGVAIDPFLSAVLVGLTRVVTTILMSFISDKFGRRPPALFSGFGMACCMFGLAACAVFPVADTQYNWIPTALLVAFIFTATLGFLTLPFSMNAEVYPTKIRGFASGLTIFFGYTMSFIILKSYPTMVSAIGNENVFLFFGLVALLGIVFVYFFLPETKGRTLQDIEDYFRGGKEANASDADKQASKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9I3BDK9</accession>
    <name>A0A9I3BDK9_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Serine/threonine-protein kinase receptor</fullName>
            <ecNumber evidence="16">2.7.11.30</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2023-03" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-threonyl-[receptor-protein] + ATP = O-phospho-L-threonyl-[receptor-protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:44880"/>
            <dbReference type="Rhea" id="RHEA-COMP:11024"/>
            <dbReference type="Rhea" id="RHEA-COMP:11025"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.30"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="16">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 16">Membrane</location>
            <topology evidence="1 16">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.30" evidence="16"/>
    <dbReference type="AlphaFoldDB" id="A0A9I3BDK9"/>
    <dbReference type="EnsemblMetazoa" id="ACON030734-RA">
        <property type="protein sequence ID" value="ACON030734-PA"/>
        <property type="gene ID" value="ACON030734"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030041"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004675">
        <property type="term" value="F:transmembrane receptor protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071363">
        <property type="term" value="P:cellular response to growth factor stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14143">
        <property type="entry name" value="STKc_TGFbR1_ACVR1b_ACVR1c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23598">
        <property type="entry name" value="TFP_LU_ECD_Babo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000304">
        <property type="entry name" value="Receptor protein serine/threonine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000023">
        <property type="entry name" value="Receptor protein serine/threonine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.60.10">
        <property type="entry name" value="CD59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000472">
        <property type="entry name" value="Activin_recp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003605">
        <property type="entry name" value="GS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045860">
        <property type="entry name" value="Snake_toxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000333">
        <property type="entry name" value="TGFB_receptor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23255:SF71">
        <property type="entry name" value="RECEPTOR PROTEIN SERINE_THREONINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23255">
        <property type="entry name" value="TRANSFORMING GROWTH FACTOR-BETA RECEPTOR TYPE I AND II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01064">
        <property type="entry name" value="Activin_recp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08515">
        <property type="entry name" value="TGF_beta_GS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00653">
        <property type="entry name" value="ACTIVIN2R"/>
    </dbReference>
    <dbReference type="SMART" id="SM00467">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57302">
        <property type="entry name" value="Snake toxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51256">
        <property type="entry name" value="GS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 15 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 16" id="KW-0460">Magnesium</keyword>
    <keyword evidence="16" id="KW-0464">Manganese</keyword>
    <keyword evidence="13 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 15 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14 16" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 16" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="4 16" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="286"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="GS" evidence="19">
        <location>
            <begin position="338"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="18">
        <location>
            <begin position="368"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="127"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="132"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="395"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00009605"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
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            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU361271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON030734-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="659" mass="73251" checksum="75EF3EC5E911F6C2" modified="2023-06-28" version="1">MAAGGKPEPATVNRSKFPMTQVAGSSTAAASTATTRATTIVIDRKVEKRRTTSSRIAGTMMWWRKIAVSVAVPPTASRRRQCSSSRGSHRRRLHQWSVLSAALVLLLLPSFSDWSFAKASTIHRHTADDNDTSPSAGVQQAKDQQPPTGKQHSSSTENGAGGGAGALAPASLQAASPQTMTLTADKNKTKLLKCHCDICKDENFVCETDGLCFTSLTREADGSLKYSYRCVEHKELNLKHDPLWCRQKTTTEPHWMGCCKEDFCNLEIVFSYLTPAQKEESYGLRWILLLTIVAVMLLLALFVGVLMLGRRKRNSGARQILPEDSLCGASYPILNGHTTIQGLIEMSTSGSGSSGLPLLVQRSIARQIQLVDVIGKGRFGEVWRGRWRGENVAVKIFSSREECSWSREAEIYQTIMLRHENILGFIAADNKDNGTWTQLWLVTDYHENGSLFDFLTARCVDPDTMLEMAFSIATGLAHLHMDIVGTRGKPAIAHRDLKSKNILVKSNLTCCIGDLGLAVRHIVATDTVDQPSTHRVGTKRYMAPEVLDETINVSQFDSFKRADVYALGLVLWEIARRCNVDGVYDEYQLPFYDVVQPDPTIEEMRKVVCVDQHRPSIPSRWIACDTLHAISKVMKECWYQHPAARLSSLRIKKTLANLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2025-10-08" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9I3BDL5</accession>
    <name>A0A9I3BDL5_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2023-03" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_040219829.2">
        <property type="nucleotide sequence ID" value="XM_040363895.2"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON030739-RB">
        <property type="protein sequence ID" value="ACON030739-PB"/>
        <property type="gene ID" value="ACON030739"/>
    </dbReference>
    <dbReference type="GeneID" id="120947972"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039408"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="433"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="480"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="560"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="805"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="872"/>
            <end position="934"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="946"/>
            <end position="976"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1143"/>
            <end position="1175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1190"/>
            <end position="1303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="560"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="570"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="592"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="808"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="889"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="946"/>
            <end position="955"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1164"/>
            <end position="1173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1190"/>
            <end position="1204"/>
        </location>
    </feature>
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        <location>
            <begin position="1227"/>
            <end position="1244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON030739-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1303" mass="143956" checksum="3CE08788F463C957" modified="2023-06-28" version="1">MAASPGSDNNCNIYMPPLGCSVASSHDFSDYQWFTDFGSYRDGVATHQSILSALSASYNGIGELSYYEKMAKDIDANLAEIDMESFRKEDINSLLTAIPTQLRLDDAERRTRNNNRIFSTITSCTAAGLIPADMMDNSFCKSEILFSPVKESHISVDSLDMDGYPDEEDIILTCQANKDNYTIAFEQSILYSEESYYDGPEHYGKYKRMNILNNLQSRTQFIRQKESMSRSEVNYTTWSNLKRNVTTPRPPITVNPLITHISRHNRTCFVRKSFSMPNLQSEQSCSLQQVQLPSQERHQLNVSQAANGNNSDESFDRPMRTLLPMCTIPTDSENESSTNGNEINDSEQSAANEHITVQEHRESLLQAQQLQEQQPPQQQTLQQPSFNLVKLFIKQKSSSTDTCMDVSSGCWPSDSTNSGGSGDHQIHHLGNGTMAARCRKKSMHDSGKCSTTGGGSIGGRQGEEDEFQLDSLDAQINNAHVNANNNNGSDGGEDDPNEERGPPSSPQRAHGTNGGSVPGHMKLNDFNRQMHGSPRRQYKGFSMNIGGRRLLQDLLQQHNGSCAGSASPLNSSTSINNNNNNNSHNVNNNNSYKDTNSDTSRTSENLTQIFNHSGNNNTTTSTGSKTKIPIDMITKSMQTSLIGAPAANGGTATREKVRVIPPSFLDRLNELGDKQKAPVFVVYPNYALPDLGFLKTQNEVVISPLSFKETMTGAGRPLKKPRPVSMNDIENIRQREYKHVTDWKSLVFLLPTEYRKLLRHIPEVGELSMSTEKQPMFCMTPPLRRGVARGVNCDCTNLLQQNTQTYNSSSSGGSSSAAPSSGYRGSSTMLTTDSEMDGILAPSNSSNLFNNNLYVYQYDSPAEVLVPCERPPSGRTAGVAPKSILRRPQGSVRGVSNSSGSGANGQSNMKAKRSSMFEEQQGPHFPQGSREKRRSLQEHHSYNFYEDAEEEEEDYEEHRNEQKLHNSPNMIPHPRLANTPKLPSADHYHKLNKLVEMNDNDMANLAHGKHQQCVDEDLEARARAEQFLSHVPKSELKHYAEIAHILESTEMIESSEPPYDRTRLRNEVSRLLSQKRSVAFTKQQTTPTSIGGNNTTPVRQQAAPATALLRPTEIKFSTPPNSPNMSVTVAKTGHHVAGGMMAEKKPPVAQRTPSSLAGSPASGTEKEKQDKIQTNRFKRLQIQWELLSKESTKASSESQRKEIRSGGNTPTGGNGGPKSRIPRPVSYPTTRTNSDPVVSKTLKSPSRIVAPRKYGAMQSPVTTPPTPAPRTPSKTFSNVTPKKVSSAPSRIPLGNRSKKIPAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9I3BE28</accession>
    <name>A0A9I3BE28_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Nicotinamide-nucleotide adenylyltransferase</fullName>
            <ecNumber evidence="11">2.7.7.1</ecNumber>
            <ecNumber evidence="11">2.7.7.18</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2023-03" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>beta-nicotinamide D-ribonucleotide + ATP + H(+) = diphosphate + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:21360"/>
            <dbReference type="ChEBI" id="CHEBI:14649"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="EC" id="2.7.7.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>nicotinate beta-D-ribonucleotide + ATP + H(+) = deamido-NAD(+) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22860"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57502"/>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
            <dbReference type="EC" id="2.7.7.18"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from nicotinamide D-ribonucleotide: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; NAD(+) biosynthesis; deamido-NAD(+) from nicotinate D-ribonucleotide: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the eukaryotic NMN adenylyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.1" evidence="11"/>
    <dbReference type="EC" id="2.7.7.18" evidence="11"/>
    <dbReference type="RefSeq" id="XP_040233937.1">
        <property type="nucleotide sequence ID" value="XM_040378003.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9I3BE28"/>
    <dbReference type="EnsemblMetazoa" id="ACON030692-RA">
        <property type="protein sequence ID" value="ACON030692-PA"/>
        <property type="gene ID" value="ACON030692"/>
    </dbReference>
    <dbReference type="GeneID" id="120956503"/>
    <dbReference type="CTD" id="42987"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040521"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000309">
        <property type="term" value="F:nicotinamide-nucleotide adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004515">
        <property type="term" value="F:nicotinate-nucleotide adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09286">
        <property type="entry name" value="NMNAT_Eukarya"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000191">
        <property type="entry name" value="Nicotinamide-nucleotide adenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051182">
        <property type="entry name" value="Euk_NMN_adenylyltrnsfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005248">
        <property type="entry name" value="NadD/NMNAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045094">
        <property type="entry name" value="NMNAT_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00482">
        <property type="entry name" value="nicotinate (nicotinamide) nucleotide adenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12039">
        <property type="entry name" value="NICOTINAMIDE MONONUCLEOTIDE ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12039:SF0">
        <property type="entry name" value="NICOTINAMIDE-NUCLEOTIDE ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 11" id="KW-0520">NAD</keyword>
    <keyword evidence="7 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 11" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="4 11" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cytidyltransferase-like" evidence="12">
        <location>
            <begin position="12"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON030692-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="262" mass="29586" checksum="158707C5146B49FD" modified="2023-06-28" version="1">MRMTSSTKIMLIACGSFSPPTPMHFRMFEIARDHIQQMGLGQVVGGIVSPVHDSYAKKGLVSATHRCAMIKIGLKTSDWIHLSDWETQQEEWTRTRQVLQYHQNYINSYLKDTNGTINNQHIPAWIPEGIKRTAGQVQLKLLCGADLLESFATPGLWKDEDLEAILGYHGIVVISRAGSNPEQFIFNSDLLSRYRRNITIVTNWVTNDVSSTLIRKLLSRGLSVKYLLDDHVAEYIRKFGLFGTNNETDRDGSSCLRGTAQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABM2AAC5</accession>
    <name>A0ABM2AAC5_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Integrin beta</fullName>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cell membrane</location>
            <topology evidence="1 14">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the integrin beta chain family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040238870.1">
        <property type="nucleotide sequence ID" value="XM_040382936.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040238892.1">
        <property type="nucleotide sequence ID" value="XM_040382958.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040238900.1">
        <property type="nucleotide sequence ID" value="XM_040382966.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040238908.1">
        <property type="nucleotide sequence ID" value="XM_040382974.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040238916.1">
        <property type="nucleotide sequence ID" value="XM_040382982.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040238923.1">
        <property type="nucleotide sequence ID" value="XM_040382989.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_049464129.1">
        <property type="nucleotide sequence ID" value="XM_049608172.1"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON000815.R77">
        <property type="protein sequence ID" value="ACON000815.P77"/>
        <property type="gene ID" value="ACON000815"/>
    </dbReference>
    <dbReference type="GeneID" id="120959494"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1240.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.100">
        <property type="entry name" value="Cytochrome c1, transmembrane anchor, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1510">
        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013111">
        <property type="entry name" value="EGF_extracell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057073">
        <property type="entry name" value="EGF_integrin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033760">
        <property type="entry name" value="Integrin_beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015812">
        <property type="entry name" value="Integrin_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014836">
        <property type="entry name" value="Integrin_bsu_cyt_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012896">
        <property type="entry name" value="Integrin_bsu_tail"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036349">
        <property type="entry name" value="Integrin_bsu_tail_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002369">
        <property type="entry name" value="Integrin_bsu_VWA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032695">
        <property type="entry name" value="Integrin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057243">
        <property type="entry name" value="Integrin_I-EGF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10082">
        <property type="entry name" value="INTEGRIN BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10082:SF60">
        <property type="entry name" value="INTEGRIN BETA-PS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07974">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23105">
        <property type="entry name" value="EGF_integrin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08725">
        <property type="entry name" value="Integrin_b_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07965">
        <property type="entry name" value="Integrin_B_tail"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00362">
        <property type="entry name" value="Integrin_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17205">
        <property type="entry name" value="PSI_integrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002512">
        <property type="entry name" value="Integrin_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01186">
        <property type="entry name" value="INTEGRINB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00187">
        <property type="entry name" value="INB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01241">
        <property type="entry name" value="Integrin_b_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01242">
        <property type="entry name" value="Integrin_B_tail"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69687">
        <property type="entry name" value="Integrin beta tail domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69179">
        <property type="entry name" value="Integrin domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103575">
        <property type="entry name" value="Plexin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00243">
        <property type="entry name" value="I_EGF_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52047">
        <property type="entry name" value="I_EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 14" id="KW-0401">Integrin</keyword>
    <keyword evidence="11 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 17" id="KW-0732">Signal</keyword>
    <keyword evidence="5 14 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045827370" description="Integrin beta" evidence="17">
        <location>
            <begin position="27"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="766"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin beta subunit VWA" evidence="18">
        <location>
            <begin position="41"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin beta subunit tail" evidence="20">
        <location>
            <begin position="681"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin beta subunit cytoplasmic" evidence="19">
        <location>
            <begin position="791"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="95"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="95"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="109"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM01241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="SMART" id="SM01242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000815.P77"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="837" mass="92840" checksum="0E09A4DA22E02CD0" modified="2025-10-08" version="1">MLPRAGPMAAGALLLLVALVADTTLGQLSNYQLTTCPGKTTCSQCIQTTNCRWCTMPNFTHPRCHGQIEKYCPEEYTVDPSNTFQLVQGRELTKPSRRVLEGQSERESYYSSSHYQSSSSSSSSSSFQQSSYESESAAGSIVQISPQRVSLKLRLNEAFRFNVNYAQAEDYPVDLYYLMDLSKSMEDDKTILSTLGADLASEMRKITSNFKLGFGSFVDKVLMPYVSTVPKNLREPCPGCVAPYGYHNLMPLSTDANLFSQEVQRANVSGNLDAPEGGFDAIMQAIVCREQIGWREKARRLLLFSTDAGFHYAGDGKLGGVITPNDGECHLDHNGRYTHSTTQDYPSISQINLKVKQNAINVIFAVTAEELSVYEQLSRLVEGSSAAKLSNDSSNIVSLVRDQYNKISSSVEMKDNRTDNVIDVKYYSRCRNTNGALQQTNRCEGLKVGDVVTFEAHITLLKCPTDPRDWQQVLQIYPVGINESLTVDIEMLCSCPCEHPSDPEYRERADECSNAGTYKCGICECDGTYHGQRCECSAMESLLEPGMVDACRMSNASEECSGRGQCVCGVCVCERRPNPDELIDGRYCECDNFSCDRPGGLLCSGPDHGRCVCGQCECREGWTGPACDCRASNETCMPPGGGELCSGHGTCECGTCRCTVTEDGRYTGRYCEKCPTCAGRCNEFKHCVQCQQYKTGPLAEANECATNCTLFVPIPVEKVTIDEERNDNKCTFFDEDDCRFEFSYNDSDQDKVVVTAQENRECPPKVFMLGIVLAVIAVVVLIGMAVLLLWKVLTSIHDRREFARFEKERMMAKWDTGENPIYKQATTTFKNPTYAGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8UZE9</accession>
    <name>A0A6E8UZE9_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="2">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="2"/>
    <dbReference type="RefSeq" id="XP_040222252.2">
        <property type="nucleotide sequence ID" value="XM_040366318.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8UZE9"/>
    <dbReference type="EnsemblMetazoa" id="ACON001052-RB">
        <property type="protein sequence ID" value="ACON001052-PB"/>
        <property type="gene ID" value="ACON001052"/>
    </dbReference>
    <dbReference type="GeneID" id="120949189"/>
    <dbReference type="CTD" id="9099"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001658"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001052"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033140"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02674">
        <property type="entry name" value="Peptidase_C19R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000083">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF23">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE USP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="4">
        <location>
            <begin position="206"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="135"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="145"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001052-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="533" mass="61096" checksum="37D23AB62E631BB3" modified="2020-08-12" version="1">MREGYKEKEIIASDSANLHVSATIRIKPQSNIRKDANANANSIMEQKCEQVKRNVSASDKTSKPNTILTWATENPCDDVSSEKNQFNRIPKRSNVLFGNDIIRNVNKNVTEDVLIPATLNEASKETDRALNKSIETSKECEDDQATQLQPRINLNKQQTSPKTNEREDDQYPNKSPSSARLIKPSLNYERCTEVNDSLRNTREGLCGLWNIGNTCFMNSMIQCLSHTRELTSFLRGQSSSERGTSKDHLILAEYTKLIKNMWSGTQRSINPSDLKYAFSSKHRMYSGSAQQDAQEFLRFFLDSLHGALNAAIKRDPLSEIDDNMNNKDKADMLWEWYSKAENSMIKDLFVGQLRSTLKCTFCNMESTMFDPFWDLSLPLPSSSSRCKLENCLEMFIKEEIMDGIDQPTCSNCDTRRKCTKRLTIERFPRYLVIHLKRFSEARWSKLTNIVEFPTKNRALNLQPYASEDISGPIYYSLYGISNHMGSTAGGHYVAVCKHPQTQQWNEFNDNYVTEAFERNLVTSNAYVLFYERA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V0P9</accession>
    <name>A0A6E8V0P9_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Exocyst complex component Sec8</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the SEC8 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040222638.2">
        <property type="nucleotide sequence ID" value="XM_040366704.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8V0P9"/>
    <dbReference type="EnsemblMetazoa" id="ACON001694-RB">
        <property type="protein sequence ID" value="ACON001694-PB"/>
        <property type="gene ID" value="ACON001694"/>
    </dbReference>
    <dbReference type="GeneID" id="120949419"/>
    <dbReference type="CTD" id="40712"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010027"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001694"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030906"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032584">
        <property type="term" value="C:growth cone membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006904">
        <property type="term" value="P:vesicle docking involved in exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090522">
        <property type="term" value="P:vesicle tethering involved in exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039682">
        <property type="entry name" value="Sec8/EXOC4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007191">
        <property type="entry name" value="Sec8_exocyst_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048630">
        <property type="entry name" value="Sec8_M"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14146">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14146:SF0">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20652">
        <property type="entry name" value="Sec8_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04048">
        <property type="entry name" value="Sec8_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="4 5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst complex component Sec8 N-terminal" evidence="7">
        <location>
            <begin position="35"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="Exocyst complex component Sec8 middle helical bundle" evidence="8">
        <location>
            <begin position="261"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="314"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="471"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="315"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="329"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU367079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001694-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1059" mass="119197" checksum="4B32BDC3188BF54B" modified="2020-08-12" version="1">MSGCGLLVSVIKTLDASETNEQREREKLKIEREFRKTDQRLNELVSRSDGDLTRVMQLFGKVSTEVTASRERIQVVKENLQSCKQLLRCRRDELKKLYTDAIQHKYVLEMLDQITEIRKVPAQLTAFLAKKHYLHATKLLMASIETTDGKLKGVEGLNDLRQDFQNRRQQLYGKLLEELNKHLYVSSTADVLQNFQRQASGRNSQYAAGTSPFQRNVLRRSAERVEANTKARKALFEISKNGFLDVDKSEIIEDTELLDPDVNSSYFLSIIVECFALLQKVPESIESIKVQMQSELLAIVTKSTQHINSLEHLPQTHQQQQQQQQQTTLGITSTPSSSTVATCNGTPSGASSADPTQPAQPVPILELLDLVFKQFKLIANAHQQALRNYGNVIQRYNIPQVKPYDIIEYWGQAQAVLKLVLTDYLDIQNDSGDEALLLRGQFAEQSTNVNAFFSRRKTPGKKLLFRFDKSSHTADAGGPQTADGDTGGTKEHRRNLSNVSNSTPKEEALLNSSAVSANSSHLLNSSFHQHQPYYFGGKDAAAERRKRERALVCPADASLVRKIYLPLMGYIQEIESFMKCKSGQPCSLNTFLANYVKDAYLARGHNRNLQLTIESLSKAQDAWRTIISPEEMKRLGLTRPLLQNTLIVENRIAETKKLIQDLPTYSDELLKMVCSLLKTYRETCQAAYRGIVQPETEDKRIYSVAWLKDDDITRFLKSLPNWTDLKSSNARLRQLQQKRLMKTEPSEEESPTQVQQRNVREAEMLTSNLGEGGISQQEILSDVGVLKELAILQESMEWFASRITEFAHDLKKPIVNGLVAASPVTTPGSPSPAGTATIVNSPVIVKDGMIKVLINLALEFEELANTCLLVLHLEVRVQCFHYLRSSPSDRYKANNPNKNDSLEPDAKVLKLTKVLSDMDEALSSTLHPRKTKYVFEGLAHLAARILIMAANSMEYIDHSGVQRMCRNALALQQTLSSITASREVALDYARSFYEMFYLDPEEILTSIVEKGAQFTEMQYLNALQLIFRNRGIVDPAVVGTYQQKLSDLLGTKPALGVTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V2R9</accession>
    <name>A0A6E8V2R9_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Fasciclin 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8V2R9"/>
    <dbReference type="EnsemblMetazoa" id="ACON002802-RB">
        <property type="protein sequence ID" value="ACON002802-PB"/>
        <property type="gene ID" value="ACON002802"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006576"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002802"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_043331"/>
    <dbReference type="Proteomes" id="UP001105220">
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    <sequence length="855" mass="95481" checksum="2B39D0B4A5A1954A" modified="2020-08-12" version="1">MAHSQNQPRLKILPASSVQRKPVGHSLLLTCRPEVPDTNLISDLRWNDNRNMTILPKPAGQSSPLIYTESIAGGTALALIFNSLQESMAGTYFCAASYSVTEKLNAAVVVETYVAITWKDAPTDQRPLMGTDYIVRCEVTANPPATVDWLRNGDQIKSSGRYVIENRGLLIKNITEADDGVYTCRAAVMSTGELKNREIRVEVQIMPQVQRLPLVMDAVEGQSFSVMCNATGKPVPEFQWIKKGTQQNAADLDRFSVNSITGQLDISTVEQQDHDNYACIARNPAGQSESVMKLNVLIRPKIIEFINITVSENEEAVFVCKAFGRPAPEITFRRYGTVEEYSIGLQVSDDRIILEQSTDDEKGETVGTLRISKLATTDDGLYECIARNRGDVAFKVGHITVEYRPIFDHLKALPPVYTWEERPANLSCFAQAIPNATIEWRFNDRRIQDLYDQNLRIENEGLRSDLIITPRDRRYYTAYKCVANNRLGSAEHIMELREARIPEMVPQAKPRIVSATSITFDIMAPPVEPGLPLTAFSVQWKEQFQSDWNFALNRTWSPDSPYIVEGLRPQTAYSFRFAARNVVGLSQWGAYVDQSTPRRSEPETPRILHTPIQDEEEDSDPIVTSPYADHFDMRWSIPVDNGEPIDMYRIKYCPGTKVNGYWTEMEEHCIEQDIARMTNYEMRSLQADTYYRIELMAHNAIGFSKPAHVLMKTARGIDVVLRVDSPTLSSAAVIGIVIGSVVLLLLIADLLCCAFGNLGILATLCRKTKRSPSDLDDEAKLGSGSLIKERPPSPLPLPPPIVKLGATTPLEDEKQPLNTPQQPAASPLKVNSSVEYDGRVVHSRSGEIIGKNSAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V2T9</accession>
    <name>A0A6E8V2T9_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Putative receptor-type tyrosine-protein phosphatase mosPTP-1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Putative protein tyrosine-protein phosphatase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the protein-tyrosine phosphatase family. Receptor class subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8V2T9"/>
    <dbReference type="EnsemblMetazoa" id="ACON002832-RA">
        <property type="protein sequence ID" value="ACON002832-PA"/>
        <property type="gene ID" value="ACON002832"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010302"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002832"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042747"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048666">
        <property type="term" value="P:neuron development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd14549">
        <property type="entry name" value="R5-PTPc-1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Receptor-type tyrosine-protein phosphatase gamma"/>
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        <property type="entry name" value="receptor-type tyrosine-protein phosphatase zeta"/>
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        <property type="entry name" value="Tyrosine-protein phosphatase 99A"/>
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    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="FN3_dom"/>
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        <property type="entry name" value="FN3_sf"/>
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        <property type="entry name" value="Ig-like_fold"/>
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        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
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        <property type="entry name" value="Protein-Tyr_Phosphatase"/>
    </dbReference>
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        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134">
        <property type="entry name" value="RECEPTOR-TYPE TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134:SF540">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE 99A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="323"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="18">
        <location>
            <begin position="102"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="18">
        <location>
            <begin position="202"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="16">
        <location>
            <begin position="407"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="17">
        <location>
            <begin position="589"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="16">
        <location>
            <begin position="695"/>
            <end position="947"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="76"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="991"/>
            <end position="1018"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="41"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1000"/>
            <end position="1010"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00054262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00061343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00069497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002832-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1085" mass="121799" checksum="14B7054274CD5B75" modified="2020-08-12" version="1">MTSTPPPLPPTEALESTPLLPVPDHEPIASVLHPYGAGDGSADDELEDQDFEEPRVLPLKSSSSTPALVRVVPIEQAASNDEPHTSTHDPTFSADDDIEPSPPQNLTVLEVTSTTIKLTWREPEKANGAIHGYRVYYIHQNQTDLHMPILKLNEMQNSVYHYTLSNLKPFTEYRILVTAFTKKHDGKPSEVTQRTDVSGPSAPKVVNLTCHSHNALYYGWRIPQTFYNTIDLYIISYRNIAYHEFREIRITVNASIMETSLIIPNLTSNAVYEVKVRAASTSVINPKQMILGSYSEPRKISLQPNCEKAPLKSQRQAYDDYDLAVLAGIVFSCSVVLLIALALILWKKCFHAAYYYLDDPQPCQGAQAGLIDWEAPSEIGGEVRGPVAVADFAKHVAQLHADGDIGFSKEYEAIQGEALNDEYPSENSQHPENKGKNRYLNVIAYDHSRVHLRQVPGQKKHLDYINANFIDGYQKPRAFIGTQGPLPGTFDCFWRMVWEQRVAVIVMITNLVERGRRKCDMYWPKDGTETYGIIQVRLVKEDVMATYTVRTLHIKHLKMKKKKQSQMEKTVYQYHYTNWPDHGTPDHPLPVINFVKKSTTANLPDGGPIVVHCSAGVGRTGTFIVLDAMLKQIEAKGSLNVFGFLRYIRAQRNYLVQTEEQYIFIHDALVEAITSGETNIKMDAIGGLVNHLDYIDAQYKLIVSYQPKEINLTSSLKPVNAIKNRSSLVPLEGSRVHLTPKPGVEGSDYINATWLHGFRRLRDFIVTQHPVIETFKDFWQMVWDHNAQTVVLLSSVDNMSFLQFWPNESEPIESDYYRIRMVSETSEDNHIVRNFVIQSIQDDYELSVKMLENPGWPDMNNTRSIFDFAVRVHERCSEYRNGPIVVVDRYGGFQACQFCAISSLAMQLEYDQTANIYTYAKLYHNKRPGIWTSYDDIRQIYRILSYMPKDLGLLKCTELRTEFDDAAMMTATPDLYSKICSNGSINNQLTGGTPDGVGNGTTTTTTTTTTSINAPPGMTIPGVVAGTPNSVCPPMIGIGGTTPPPPATLQNGGTVIVKMNGDDNDELSVVVATGNHHLNLDHNQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V324</accession>
    <name>A0A6E8V324_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8V324"/>
    <dbReference type="EnsemblMetazoa" id="ACON002942-RC">
        <property type="protein sequence ID" value="ACON002942-PC"/>
        <property type="gene ID" value="ACON002942"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012950"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002942"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041114"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071456">
        <property type="term" value="P:cellular response to hypoxia"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010557">
        <property type="term" value="P:positive regulation of macromolecule biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23043">
        <property type="entry name" value="HYPOXIA-INDUCIBLE FACTOR 1 ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23043:SF17">
        <property type="entry name" value="PROTEIN SIMILAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="135"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="236"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="394"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="469"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="588"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="767"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="142"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="207"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="242"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="479"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="622"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="659"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="785"/>
            <end position="798"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002942-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="933" mass="95770" checksum="C82B3C427D2FCF78" modified="2020-08-12" version="1">MNKGFLMFSEEEPGLTMLKDEPDDLTHLAPTAGDACIPLEETGTFFNEMFEDFIIPDSYTLLQDELCSLDSQDTRLDASSIVVHPVSTSDSLTSSLSVASVASLPLGTAVSGTVDGAASLVSNAGTIAGINGSQTQQQHNYAPSSSASAASPSSLSSSSISVSSSPVPSSPSRSHISSNPNSPSGTSGLGSSASSTVSTVDGRRHSMTTIGSSNHQHNATSNDPFINYRDEVNEVSPSPHLLSPGISKSPESSSLPSLCSPNGSLPEDELAFMSLNMDELDLSMRAPYISMSEVDDLPLLTSDDLLWGVPAAAIEGNATGTSISAFGLSGKFFIKDEFTAIGSAANGTETGSSSLADPSAASLPHTVQHSLPVDKEHSLLLSAVVDTIAANGAGSVAGSSSGSNDLPTTNNSNNDSKSTAPSAASSAASIDSSLAALLCGGNVISIQSDTSNLSQQQSIGNQLLISHTNAGSQQEQRHNNQPQPQQSPLQQQQQQSSLNGSQQRQQEAGPKRIKLSEHNLLPHSMVVLPANVKLCDTWKFDDLLQLNGGSISINTKPGTQQQQQQQQQAIVPQTNIIASFVTTAVKKPSPTGVNGNNKRTLAGVSESADDTAAGNNCKRLKSSSGAGPAPSAPQLLQQLMAPSPVPQKLKSKSRSAQDGGAGGRWPNGHAGGNAGLLQEQSGNSVLMNLLVSGCDKTIPLTPPEILIENTDMVELSSDGNTTRRDMLFNLGDEDDNGVLFSSTNPLIGGQAPLNDALARPLTIQTEQQHGTASGAERGCTSAQGPIGGTPGKLFGGSGASATAPNSQFSMLVSPSLLTPTDLEIWRAIQQGQLDPKKIPSGYGSGTAFGAAGHIPKSLSSTPTAQLLSAADLLGGDCAGANLLAELENELVGGGAAAGLSDVGAGSNNGRNAAFRTIAATEEEGALLQQGMLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V334</accession>
    <name>A0A6E8V334_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transcription initiation factor IIA subunit 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TFIIA subunit 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8V334"/>
    <dbReference type="EnsemblMetazoa" id="ACON002958-RA">
        <property type="protein sequence ID" value="ACON002958-PA"/>
        <property type="gene ID" value="ACON002958"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_008357"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002958"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_036998"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005672">
        <property type="term" value="C:transcription factor TFIIA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07976">
        <property type="entry name" value="TFIIA_alpha_beta_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.18.10:FF:000002">
        <property type="entry name" value="Transcription initiation factor IIA subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.18.10">
        <property type="entry name" value="Transcription factor IIA (TFIIA), beta-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004855">
        <property type="entry name" value="TFIIA_asu/bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009088">
        <property type="entry name" value="TFIIA_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12694">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIA SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12694:SF8">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIA SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03153">
        <property type="entry name" value="TFIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01371">
        <property type="entry name" value="TFIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50784">
        <property type="entry name" value="Transcription factor IIA (TFIIA), beta-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="47"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="121"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="124"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002958-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="226" mass="25157" checksum="524A5B7E36837025" modified="2020-08-12" version="1">MVDETPEVEVSPISYKLVELSPNEPLERYRLDRQLISGLVAGQRTNRWSASAKESNEPIACSSSSSSSSRRGVRSKASAAARNARRRHPFRAAQAKAVPVLSEQEQALTFHICSSLQLDGAVDTSDEEGSDISDDNIVDDDDEDLDKEEEDDMEAEGGAEEEPLNSEDDVTDEDASDLFDTDNVVVCQYDKITRSRNKWKFYLKDGIMHIGGKDYVFQKSNGDAEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V4X3</accession>
    <name>A0A6E8V4X3_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="4">N-acetyltransferase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8V4X3"/>
    <dbReference type="EnsemblMetazoa" id="ACON002285-RA">
        <property type="protein sequence ID" value="ACON002285-PA"/>
        <property type="gene ID" value="ACON002285"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009461"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002285"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034921"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050769">
        <property type="entry name" value="NAT_camello-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13947">
        <property type="entry name" value="GNAT FAMILY N-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13947:SF37">
        <property type="entry name" value="LD18367P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="66"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetyltransferase" evidence="3">
        <location>
            <begin position="141"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002285-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="236" mass="27160" checksum="202DA264FD0AF35E" modified="2020-08-12" version="1">MKPFVVIRKHENQDNVALQRLIREFVMSGAWEAFVSCLFREITLQLIVLGWALMFIFFGIPLYMCALAIPCVILLIWFTVYGSYYNKSTELALRPSKLCWVAEIYEPLLAMNAKAQAANIERCYTDRPGPEVQQELGKMRKNIIGTISCRNHLAMDNAGFITRLAVSPKYGLTPVTEYLIQRVLQYASDSQLYAIETVTSECDQDVREIYLKIGFNIRQVYHKQIVGAIKRRCKPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V5B7</accession>
    <name>A0A6E8V5B7_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">PDZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8V5B7"/>
    <dbReference type="EnsemblMetazoa" id="ACON002576-RB">
        <property type="protein sequence ID" value="ACON002576-PB"/>
        <property type="gene ID" value="ACON002576"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001267"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002576"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035471"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044233">
        <property type="term" value="C:mitochondria-associated endoplasmic reticulum membrane contact site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051560">
        <property type="term" value="P:mitochondrial calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990456">
        <property type="term" value="P:mitochondrion-endoplasmic reticulum membrane tethering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20825">
        <property type="entry name" value="C1_PDZD8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21674">
        <property type="entry name" value="SMP_PDZD8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041489">
        <property type="entry name" value="PDZ_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039275">
        <property type="entry name" value="PDZD8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058801">
        <property type="entry name" value="PDZD8_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031468">
        <property type="entry name" value="SMP_LBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21519">
        <property type="entry name" value="PDZ DOMAIN-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21519:SF1">
        <property type="entry name" value="PDZ DOMAIN-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17820">
        <property type="entry name" value="PDZ_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26547">
        <property type="entry name" value="PDZD8_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51847">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="6" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="SMP-LTD" evidence="12">
        <location>
            <begin position="5"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="11">
        <location>
            <begin position="256"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="10">
        <location>
            <begin position="621"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="380"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="417"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="800"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="920"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="737"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="390"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="923"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="951"/>
            <end position="961"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002576-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="979" mass="108051" checksum="8261D5DDC3954D5F" modified="2020-08-12" version="1">MAINLILQFLYFELRQSNRVRKWFHRKLSLELDELISKTTTGKLFDKLTIRDLDLGSQFPEIKNFRLHAVDLHPDEGHIESLDVLLDLHYEGNFRLTIDADMVLGKKGSLALRVKQVSGLARLQFTRKPYTHWSLSFINDPKVELDVQSHFQGRQMQSNITSLISNQIRKAIRRKHTLPNYKLRYKPFFHRVEEDYELNEIVPNGSLEVTIAEISRLNAPIRTLTHVYCTLTLAHMPWVVARQQDDQGATMIVSLDIEIHKAKNQQIGIVFKQSDQTVLIDAVIPNTPASKAELRRGDVLLSIQGKRVTNISQVPKVIKTLNRPMFVLRIERLVPGQIRNDALLDDYEPEFEEIDPNLNITFVKNVEAVQIGGGTAVLRERKLARRNSKDNSGGESSHSNTPGTTPVKKSSTIVVGGAETSTPRGSFSEPAAAGGPKPKALNVECYPQHSSIDAEFNSFIRLDDPCTFQLMEKYSYLNLSVFGKSNEEHRLLGYLNIPINSILVECNESHLGHHLKKYPLLPPEAIDVSNHPLSMQSGFDQNYCYGDVLLSFVWDGSALASLSSPTAAASKAISAENKKQRLITISRGSADKLDDDRTDGLPLDAKPSASSTFTQLPAQQQHDFIRTHFHRTTQCDYCGKKIWLKDAVQCRDCAMCCHKKCINKCQSSTVCTANESSAGTASDRSSTNSSTSTGIIMAAGAAASMASTAAATVGTNQQPEFKVTEPESPTIEVEDFVDIAEEQQQQQLQQQQQQQQQQQQQQQQQQQIVPKSKLETHRQSFSDLLVQGLKRVNSANNLSIPTMGGLNPSSKSLPPTPQHTPRKQSLANVNANPFLLVTQRLESLPEDVNELNMEQIVDLTAPLIEYGPSDTLMALAKSSSKAMYGDCEPDVRTEKINKLLSKLHIALDYETINQSVLNATKESSSSSSGSGAGANSKAAAAAAARQHQIGLPGGPVGGAGAGAQHHHAAPLLRPAVRPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V5E2</accession>
    <name>A0A6E8V5E2_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="3">C-type lectin domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8V5E2"/>
    <dbReference type="EnsemblMetazoa" id="ACON002625-RA">
        <property type="protein sequence ID" value="ACON002625-PA"/>
        <property type="gene ID" value="ACON002625"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002318"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002625"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041147"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd00037">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050828">
        <property type="entry name" value="C-type_lectin/matrix_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45710">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN-CONTAINING PROTEIN 180"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45710:SF26">
        <property type="entry name" value="RH26557P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C-type lectin" evidence="2">
        <location>
            <begin position="153"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="234"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="234"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="251"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002625-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="345" mass="37479" checksum="B82D32139A9B3FC1" modified="2020-08-12" version="1">MQATVVAAAGPAPKYIPTSSFYINKRIGEALELDPQHHAAHSPHRPRPPVGKVIASTGAGGLYGDNQLAAGAGHGATFTPMRQTFGGASPTELLASPREVSETDLYLLGAIEKLVYRVDYMENRLRRAEQIIYFLMAGNSQKQEPCPQNFTQVHDRCYHFDIERGLNWKSASTMCKSYGAHLAEFETVAEFQDVVAYILNNPVNRGKDFWLGGLNPGLLWIWANSAKPVNPNTNLSSITSSSSNKPGSKGTASTKPTNLANNGTELPSGGTKIVNNPSASKLPTLEITGSGRCLRLSYNSALYTYGYRGEDCSAQFSYVCELKDKSLDNEISRIAKQLKLADDVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V9J9</accession>
    <name>A0A6E8V9J9_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">YLP motif-containing protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Nuclear protein ZAP3</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Plays a role in the reduction of telomerase activity during differentiation of embryonic stem cells by binding to the core promoter of TERT and controlling its down-regulation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Interacts with PPP1CA and NCOA5. Forms a complex with ILF2, ILF3, KHDRBS1, RBMX, NCOA5 and PPP1CA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus speckle</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8V9J9"/>
    <dbReference type="EnsemblMetazoa" id="ACON001168-RA">
        <property type="protein sequence ID" value="ACON001168-PA"/>
        <property type="gene ID" value="ACON001168"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_003576"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001168"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040948"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032204">
        <property type="term" value="P:regulation of telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000399">
        <property type="entry name" value="YLP motif containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058903">
        <property type="entry name" value="Spectrin_YLPM1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026314">
        <property type="entry name" value="YLP_motif_con_p1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13413">
        <property type="entry name" value="YLP MOTIF CONTAINING PROTEIN NUCLEAR PROTEIN ZAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13413:SF0">
        <property type="entry name" value="YLP MOTIF-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26583">
        <property type="entry name" value="Spectrin_YLPM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="2" id="KW-0488">Methylation</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="YLPM1-like spectrin repeat" evidence="14">
        <location>
            <begin position="144"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="116"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="186"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="398"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="676"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="762"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="865"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1127"/>
            <end position="1169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="121"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="204"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="221"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="255"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="337"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="430"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="502"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="694"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="726"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="763"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="791"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="805"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="867"/>
            <end position="879"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00058677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00065932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00068971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00083294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF26583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001168-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1169" mass="130601" checksum="19828894D0152DC3" modified="2020-08-12" version="1">MSWQNNWSAMPPPAMNPQYPPPLPTTHTMPPAVPLPGYGAVNTDQYNQWQWQQYQQQYAQWYAMYGEKYAQQTGNALPPVAAPMPQSVPNVLANPYVAAPLPVGPAFGSMSMVAPPPPSEPHPDELRMGRITEKPPPPEEKSPEEIAFDEQFRKWEQEFETWKRTNINHPDKAAYREYEQKYEEQRKKLLDHREQLRRRKRAHSAASQPPHSSALSISLQKPPPPPPQPAQPPPQPPSEPPQPSQPPRPPGQEPTNCSSNTTPASTGGMAEENTHHAGSDAANSMHLHNLFSSPSKAGRDGGIPGLDLIDETPQPVPKKAKLDENVIDLSNEEDESKEPKPKETKPSPDNMISTLLNDPNVNALLQLVGKTISANGAGGSASGANAPNPLVAALAQLTSNTNPATLEPSMPNERPANGMVERNEPHPNASRFNGSKEQPPSTMQSGGGGTTEECPAAGFRNRPQGFNPYVPPPLVDVDLSRPPPMLLPPGNSRPQRDRSSRNSDATSENGSFNDGYSGDGSPLPKIPRFDRPPPTFAANHPEYEIDPELGRPVAVPKPQWMTEEEYQEIYDRYEHVQSFDERKNKMLLAIQVLKTSKLRAGMLLEPPPDEVNRVPVAMPKPLPPVKTPIKSEPVDDYFQPQQVFDYSNCRNTRPVASQQLPTGRVIDYGHRGVNQYTANNNRLNNNSRRDEFRGMNNNARGGANENGFGTNTQRFDYNHSRVANAPPTTAPLQQNQQWPLSNQSAAQDRSVTGLERLAQMHLNPNPEESNPNYPSKFLLMNDNRPNCLSTKRGKRPSSIRKQKLKQLQQQQQEQQQQQQLDQQQQPQQQSQQPEPKAGQPEPEPAQSEAMPIPIVCKQEPEMHLEDLSSDEDGLPDGDDGFAYQSNDECVKPAIKQELLDDRPESPVSSLSSQNLSPIQMIDIEDLLLPPGRYQRPLRICFLIRGLPGSGKSHLARLIKNKEQSFGQSPRVLSLDDYFLVDKEEEEKDPVTGRITKLTRSLYEFDAEMEDVYVQNLLKAFKRTISERLFDFVIVDCCNHRLETYCEFHNYARSNGFKVYTCTMQTDVEDCARQNIHNRTEAEIQAYADNWAMAPDDHVQINFNSLLEPELQEETDTMVADMELVGADEEEPYGADDQGLLEQISTSHDDSNDSFADGTAERGWMVPANP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V9L4</accession>
    <name>A0A6E8V9L4_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="10">BTB domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_040220979.1">
        <property type="nucleotide sequence ID" value="XM_040365045.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8V9L4"/>
    <dbReference type="EnsemblMetazoa" id="ACON001073-RA">
        <property type="protein sequence ID" value="ACON001073-PA"/>
        <property type="gene ID" value="ACON001073"/>
    </dbReference>
    <dbReference type="GeneID" id="120948551"/>
    <dbReference type="KEGG" id="acoz:120948551"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002202"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001073"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035382"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048813">
        <property type="term" value="P:dendrite morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030707">
        <property type="term" value="P:follicle cell of egg chamber development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061061">
        <property type="term" value="P:muscle structure development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007423">
        <property type="term" value="P:sensory organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18315">
        <property type="entry name" value="BTB_POZ_BAB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000118">
        <property type="entry name" value="Abrupt, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051095">
        <property type="entry name" value="Dros_DevTransReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110">
        <property type="entry name" value="BTB DOMAIN TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110:SF106">
        <property type="entry name" value="FI01104P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="8">
        <location>
            <begin position="31"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="432"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="458"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="291"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001073-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="499" mass="55985" checksum="C62538E47E234A6E" modified="2020-08-12" version="1">MNPQQFSLRWNNYTSYIAGAFDSLRYEEDFVDVTLCCEGRKIRAHKILLSACSPYFKDVFKENPCQHPVIIFKNVRYTDLMSLVEFMYQGEVSVPQEQLPSFLHTAEILAIRGLTDNTSDSRQPASTTTSAIAQQLIQSQAVLEKPATITTTDSLYLTLPSNSTIVHQPKLVQAQIQTTPIITKPLIKNTPTEMPSLAASSTLAVPIAQQQQPAIAKIQVQASSQSQQPQQVQVQVQQVQQTQTHQTQQATTSQTQSQSQQATTLQEVVENVVLPRKKKVKGQQIIASQSNTSIPSGSTVTVTTTTTGQSGDNEMYETDTYTITTKDAREDHEQAEQYVESGQSSGTTLKMEIPEFISVGEPINTGNGTNNTYLQESYELVSENMEDKDEEEDMAQDNIEIEGSEVDMSRLFQGSTDEQTKSNILQDTELRFRCSICWKGFKHPMSLTLHKDLHSGKTKCPVCLRSFSRSYDMRIHLNKIHQVTLKLDARFNFKNMKSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V9L9</accession>
    <name>A0A6E8V9L9_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Small acidic protein-like domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8V9L9"/>
    <dbReference type="EnsemblMetazoa" id="ACON001208-RC">
        <property type="protein sequence ID" value="ACON001208-PC"/>
        <property type="gene ID" value="ACON001208"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000209"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001208"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038401"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028124">
        <property type="entry name" value="SMAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22426">
        <property type="entry name" value="ARGININE_SERINE-RICH COILED-COIL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22426:SF2">
        <property type="entry name" value="ARGININE_SERINE-RICH COILED-COIL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15477">
        <property type="entry name" value="SMAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Small acidic protein-like" evidence="2">
        <location>
            <begin position="392"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="416"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="202"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="224"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="432"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF15477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001208-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="505" mass="56945" checksum="CAFC64A5D5F831CC" modified="2020-08-12" version="1">MKADRKDKRKSSPPRHDASKHDRSQSRERERKNRDKDRERERDRTREREREQRNRDRDRERERQKDRPKDRNRDRERERERERDRDRERSRYDRDRGSARDSRSDYHRDQRRGGGNWSKSNERDNNRRDSRGYDRKDDRKDRRSGRDYDKDHRRSRRSESRESRHRRRHSPNERVPSSRARDSRSRSRSRSRSGAKSSPPRTSQQQTPSTNASYAGAGLLPTPSRFTQSPTTKPVSLLPTPPVAQSLLGAAPSLVPTAGIPSLMSIPTKPPISSLIPPAVAAKLTLSNESKLGQLALPLTTPPAQQDPAVTSVLLSRSALLRNTAKAAQLEKMGIDVLQQSQKAVDSVPLPSYYNPGVVNPVRYADQVQKRKLLWSHKTTESKEVSSNISKWEQAKFAQDTDGRVASKFLRLMGVKDAAPKPGEEGESPVPTGGTSSASLSSKVGSSSGATGASGPSNGGKSTGDSIKKQEELFSTMEQQYEVARQVTHTMRGVGLGFGSQARQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VAN7</accession>
    <name>A0A6E8VAN7_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Oxidation resistance protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the OXR1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VAN7"/>
    <dbReference type="EnsemblMetazoa" id="ACON001751-RE">
        <property type="protein sequence ID" value="ACON001751-PE"/>
        <property type="gene ID" value="ACON001751"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005654"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001751"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034408"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006571">
        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354:SF62">
        <property type="entry name" value="MUSTARD, ISOFORM V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354">
        <property type="entry name" value="NUCLEOLAR PROTEIN 7/ESTROGEN RECEPTOR COACTIVATOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07534">
        <property type="entry name" value="TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00584">
        <property type="entry name" value="TLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51886">
        <property type="entry name" value="TLDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026065230" description="Oxidation resistance protein 1" evidence="5">
        <location>
            <begin position="22"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="TLDc" evidence="6">
        <location>
            <begin position="131"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00040604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001751-PE"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="292" mass="32909" checksum="E74459D056F573CB" modified="2020-08-12" version="1">MWYLVLVALSATLYFASKRWGRQLYYRWGRLIPFRRAIGNKSRPPTPPLIDTSVPAHLPADGVSGSGEEGYPLLGGKEEFDDGRLHTVWFGSVLEFNVLSMSTDDYRKATLFASGSFDQDFQIPDLVGQTEILSEEHREKLCAHLPARAEGYSWSLVFSTSQHGFSLNSLYRKMHKLESPILIVIEDTDHNVFGALTSCSLHVSDHFYGTGESLLYKFNPHFKVFHWSGENLYFIKGNPESLAIGAGDGKFGLWLDGDLNQGRSQHCSTYSNEPLAPQEDFVIKTLECWAFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VB00</accession>
    <name>A0A6E8VB00_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the troponin T family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040222828.1">
        <property type="nucleotide sequence ID" value="XM_040366894.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VB00"/>
    <dbReference type="EnsemblMetazoa" id="ACON002350-RA">
        <property type="protein sequence ID" value="ACON002350-PA"/>
        <property type="gene ID" value="ACON002350"/>
    </dbReference>
    <dbReference type="GeneID" id="120949532"/>
    <dbReference type="CTD" id="136032042"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011848"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002350"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041535"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005861">
        <property type="term" value="C:troponin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005523">
        <property type="term" value="F:tropomyosin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006936">
        <property type="term" value="P:muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006937">
        <property type="term" value="P:regulation of muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045214">
        <property type="term" value="P:sarcomere organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.350:FF:000003">
        <property type="entry name" value="Troponin T isoform 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027707">
        <property type="entry name" value="TNNT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001978">
        <property type="entry name" value="Troponin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038077">
        <property type="entry name" value="Troponin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11521">
        <property type="entry name" value="TROPONIN T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11521:SF1">
        <property type="entry name" value="TROPONIN T, SKELETAL MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00992">
        <property type="entry name" value="Troponin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90250">
        <property type="entry name" value="Troponin coil-coiled subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="39"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="202"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="288"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="13"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="39"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="60"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="112"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="139"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="202"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="294"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="306"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002350-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="361" mass="43548" checksum="BD9E5F72520907D6" modified="2020-08-12" version="1">MSDDEEYSGDDPEFIKRQDQKRSDLDEQLKEYINEWRKQRAKEEDELKKLKDKQAKRKVSRAEEEQRMAQRKKEEEERRVREVEEKKQREIDEKRKRLEEAEKKRQAMLQAMKDKDKKGPNFTITKKDSSFGMSNAQMERNKTKEQLEEEKKISLSFRIKPLEVDGLSADSLRARATELWETIVKLETEKYDLEERQKRQDYDLKELKERQKQQLRHKALKKGLDPEALTGKYPPKIQVASKYERRVDTRSYDDKKKLFEGGYSAYYQEKINKKWAQRQELFMARTKTKLPKWFGERPGKKSGDPETPEGEDEVKPDDEEVEEVEEVVEEVVEEEEEEEEEEEEEEEEEEEEEEEEEEEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VB03</accession>
    <name>A0A6E8VB03_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="9">MFS domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VB03"/>
    <dbReference type="EnsemblMetazoa" id="ACON001980-RA">
        <property type="protein sequence ID" value="ACON001980-PA"/>
        <property type="gene ID" value="ACON001980"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000362"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001980"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034274"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050930">
        <property type="entry name" value="MFS_Vesicular_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23506">
        <property type="entry name" value="GH10249P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23506:SF26">
        <property type="entry name" value="MFS-TYPE TRANSPORTER SLC18B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
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        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026233210" evidence="7">
        <location>
            <begin position="25"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="48"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="78"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="108"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="143"/>
            <end position="165"/>
        </location>
    </feature>
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            <begin position="171"/>
            <end position="192"/>
        </location>
    </feature>
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            <end position="239"/>
        </location>
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        </location>
    </feature>
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            <end position="305"/>
        </location>
    </feature>
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            <begin position="311"/>
            <end position="330"/>
        </location>
    </feature>
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            <end position="369"/>
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    </feature>
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        <location>
            <begin position="381"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="12"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001980-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="494" mass="52493" checksum="7120D3A9323F014B" modified="2020-08-12" version="1">MALDFTRRQWLTLMIMGLADFCNAICVSLQAPFFPNEAESKGATATEYGLVFGIFELVVFIISPLYGQYINRIGPKVLFNSGIFTTGTSAILFGLLDRVPGHVPFITLAFVIRIVEALGNAAFLTASFAIIAKEFPNNVATTFASLETCFGLGLIVGPMVGGALYTVGGYYLPFVVLGSALFVTAILTLCILPKHPNEQQPNREKSASLLAVLKIPGVVVCALAISATSASIGFLSATMEPHLRQFDLSPILLGVVFVINGGVYALTAPIWGWGVDKFLNPKVVSTVGCFLVVGGFCMVGPASFIPLETNLTYVIIGLVLHGLGIAAVLVSSFTDALNISIKKGLPDSIETYGMISGLWTSTFAFGAFVGPSVSGLLFDAIGFRASTVFIIGLQLVVGIITILFLCFERSPAPYKEISSVESLIKPATSAQDADSHNESGASRTSSLDITYRGNKSGFSGSQSSLIGSWRPSVNNLLISNSYQSKQGHWARSVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VB04</accession>
    <name>A0A6E8VB04_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">Glutamine synthetase</fullName>
            <ecNumber evidence="3 11">6.3.1.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 11">
            <text>L-glutamate + NH4(+) + ATP = L-glutamine + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16169"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.1.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9 10">Belongs to the glutamine synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.1.2" evidence="3 11"/>
    <dbReference type="RefSeq" id="XP_040221967.1">
        <property type="nucleotide sequence ID" value="XM_040366033.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VB04"/>
    <dbReference type="EnsemblMetazoa" id="ACON002355-RA">
        <property type="protein sequence ID" value="ACON002355-PA"/>
        <property type="gene ID" value="ACON002355"/>
    </dbReference>
    <dbReference type="GeneID" id="120949061"/>
    <dbReference type="CTD" id="32087"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007115"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002355"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030328"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004356">
        <property type="term" value="F:glutamine synthetase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006542">
        <property type="term" value="P:glutamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.70:FF:000004">
        <property type="entry name" value="Glutamine synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.590.10:FF:000011">
        <property type="entry name" value="Glutamine synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.70">
        <property type="entry name" value="Glutamine synthetase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.590.10">
        <property type="entry name" value="Glutamine synthetase/guanido kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008147">
        <property type="entry name" value="Gln_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036651">
        <property type="entry name" value="Gln_synt_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008146">
        <property type="entry name" value="Gln_synth_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027303">
        <property type="entry name" value="Gln_synth_gly_rich_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027302">
        <property type="entry name" value="Gln_synth_N_conserv_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050292">
        <property type="entry name" value="Glutamine_Synthetase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20852">
        <property type="entry name" value="GLUTAMINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20852:SF57">
        <property type="entry name" value="GLUTAMINE SYNTHETASE 2 CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00120">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03951">
        <property type="entry name" value="Gln-synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01230">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54368">
        <property type="entry name" value="Glutamine synthetase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00180">
        <property type="entry name" value="GLNA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00181">
        <property type="entry name" value="GLNA_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51986">
        <property type="entry name" value="GS_BETA_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51987">
        <property type="entry name" value="GS_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GS beta-grasp" evidence="13">
        <location>
            <begin position="31"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="GS catalytic" evidence="14">
        <location>
            <begin position="118"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="292"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="297"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002355-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="368" mass="41147" checksum="565E4664D06AC08A" modified="2020-08-12" version="1">MSRTLELSPNAHINKSLLDKYMALPMPDGKIQATYIWIDGTGENVRCKDRTLDFIPQSPSELPIWNYDGSSTYQAEGSNSDVYLHPVAIYRDPFRRGNNILVLCETYRYDGTPTESNKRKACLEVCERAAEEQPWFGIEQEYTLLDVDGRPLGWPKNGFPGPQGPYYCGVGADKVYARDIVDAHYRACLYAGVKICGTNAEVMPAQWEYQVGPCEGIQIGDDLWMSRFLLHRVAEEFGIVATLDPKPMQGDWNGAGAHTNVSTKTMREPGGLAEIENAISKLAKCHERHIRAYDPNGGKDNERRLTGKHETSSIHDFNAGVANRGCSIRIPRGVSDQGFGYFEDRRPSSNCDPYSVAEAILRTIILNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VB06</accession>
    <name>A0A6E8VB06_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Hemolymph juvenile hormone binding protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the TO family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VB06"/>
    <dbReference type="EnsemblMetazoa" id="ACON001981-RD">
        <property type="protein sequence ID" value="ACON001981-PD"/>
        <property type="gene ID" value="ACON001981"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010069"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001981"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034863"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007623">
        <property type="term" value="P:circadian rhythm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.15.10.30:FF:000001">
        <property type="entry name" value="Takeout-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.15.10.30">
        <property type="entry name" value="Haemolymph juvenile hormone binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010562">
        <property type="entry name" value="Haemolymph_juvenile_hormone-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038606">
        <property type="entry name" value="To_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11008">
        <property type="entry name" value="PROTEIN TAKEOUT-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11008:SF35">
        <property type="entry name" value="PROTEIN TAKEOUT-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06585">
        <property type="entry name" value="JHBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00700">
        <property type="entry name" value="JHBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00060902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001981-PD"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="176" mass="19896" checksum="A38734BA321B1B8A" modified="2020-08-12" version="1">MENNAGAVRIKALFTDIVAQGAGNYTIKEVRSDLKKLRIDMSIAIPRVETRGKYEVIGNVLLLPVRSNGEFWTEFSDITAIAKIYGKAVERDGESFMGIEKINVDFTMKNARFKVKDHVNTQNVLGEAINQFLNQNANELIQEMRPAASQSIGKLFRKFLNDAFTNLPTRLWLLDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VEV1</accession>
    <name>A0A6E8VEV1_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Tropomyosin</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the tropomyosin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040230177.1">
        <property type="nucleotide sequence ID" value="XM_040374243.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VEV1"/>
    <dbReference type="EnsemblMetazoa" id="ACON001797-RH">
        <property type="protein sequence ID" value="ACON001797-PH"/>
        <property type="gene ID" value="ACON001797"/>
    </dbReference>
    <dbReference type="GeneID" id="120953866"/>
    <dbReference type="CTD" id="41852"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002131"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001797"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039487"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000005">
        <property type="entry name" value="Tropomyosin alpha-1 chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000001">
        <property type="entry name" value="Tropomyosin alpha-1 chain isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000533">
        <property type="entry name" value="Tropomyosin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19269">
        <property type="entry name" value="TROPOMYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00261">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00194">
        <property type="entry name" value="TROPOMYOSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00326">
        <property type="entry name" value="TROPOMYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="13"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001797-PH"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="254" mass="29510" checksum="869BE21926330F22" modified="2020-08-12" version="1">MAANVQQQSGNLLDVLKKKMRQTKEEMEKYKDECEEFHKKLQNEVMRREEAESEVAALNRRIQLLEEDLERSEERLASATAKLSEASAAADESERIRKALENRTNMEDDRVGILEAQLAQAKLIAEEADKKYEEVARKLVLMEQDLERSEEKVEMNESKIVELEEELRVVGNNLKSLEVSEEKATQREESYGGQVRVLDQRLKEAEARAEFAERSVQKLQKEVDRLEDDLTVERAKNKMLQEEMEATLHDIQNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VEV2</accession>
    <name>A0A6E8VEV2_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Tropomyosin 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the tropomyosin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040230181.1">
        <property type="nucleotide sequence ID" value="XM_040374247.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VEV2"/>
    <dbReference type="EnsemblMetazoa" id="ACON001799-RB">
        <property type="protein sequence ID" value="ACON001799-PB"/>
        <property type="gene ID" value="ACON001799"/>
    </dbReference>
    <dbReference type="GeneID" id="120953868"/>
    <dbReference type="KEGG" id="acoz:120953868"/>
    <dbReference type="CTD" id="41853"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011980"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001799"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039771"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000005">
        <property type="entry name" value="Tropomyosin alpha-1 chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000001">
        <property type="entry name" value="Tropomyosin alpha-1 chain isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000001">
        <property type="entry name" value="Tropomyosin alpha-1 chain isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000533">
        <property type="entry name" value="Tropomyosin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19269">
        <property type="entry name" value="TROPOMYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00261">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00194">
        <property type="entry name" value="TROPOMYOSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00326">
        <property type="entry name" value="TROPOMYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="55"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="12"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001799-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="284" mass="32410" checksum="471B1EFA5BCF199A" modified="2020-08-12" version="1">MDAIKKKMQAMKIEKDNAQDKADTCENQAKEANLRADKIMEEVADLTKRLEQVTQDHEKFKASLEQATKDSEEKDKLLTSTEANVAALTRKVQQVEEDLEKSEERSSAALSKLLEATQSADENNRMCKVLENRSQQDEERMDQLSNQLKEARMLAEDADTKSDEVSRKLAFVEDELEVAEDRVKSGEAKIMELEEELKVVGNSLKSLEVSEDKANQRVEEFKRQLKSLTIKLKEAETRAENAEKNVKKLQKEVDRLEDELGVNKDRYKSLADEMDSTFAELAGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VF78</accession>
    <name>A0A6E8VF78_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="1">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.1.3.48" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8VF78"/>
    <dbReference type="EnsemblMetazoa" id="ACON002438-RB">
        <property type="protein sequence ID" value="ACON002438-PB"/>
        <property type="gene ID" value="ACON002438"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002913"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002438"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039278"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004726">
        <property type="term" value="F:non-membrane spanning protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001784">
        <property type="term" value="F:phosphotyrosine residue binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048666">
        <property type="term" value="P:neuron development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14544">
        <property type="entry name" value="PTPc-N11_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000121">
        <property type="entry name" value="Corkscrew, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000237">
        <property type="entry name" value="Protein-tyrosine phosphatase SHP-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052123">
        <property type="entry name" value="Non-rcpt_Tyr_Phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46257">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE CORKSCREW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46257:SF3">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE CORKSCREW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0727">SH2 domain</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="6">
        <location>
            <begin position="77"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="7">
        <location>
            <begin position="360"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="145"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="145"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002438-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="481" mass="55282" checksum="CDD1E84AE5906B35" modified="2020-08-12" version="1">MIRWHEKMYDVGGGQKFATLCDLIEHYKRNPMVETCGTVVHLRQPFNATRITAAGINDRVEQLQRENGGQSYGKGGFWEEFESLQQQECRHTFSRREGQRNENRAKNRYKNILPFDHTRVKLKDVDSTVPGADYINANYIRQPTEAEQNDMSSSSENLAQSAGSALNNGYSQPNATNNNGTNGNSACQGYSAGSVAPTKNCSSCQLLNKPCSQCSLKASSEMKHKRNESITSRMQLQQQSPHKRDEKDMFKTYIATQGCLTNTIQDFWNMIWQENTRVIVMTTKEMERGKKKCEKYWPDPQQSKEWGHARVRCLSETSTADYTLREFLLSWRGQEDRKIYQYHFQVWPDHGVPADPGCVLNFLQDVNTRQAQLQLEGLMPGPICVHCSAGIGRTGTFIVIDMILDQILRQGHECEIDIQRTIQMVRSQRSGMVQTEAQYKFVYFAVQHYIQTLLQRKQAEQQSLQVGREYTNIKYVDEIYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VFD2</accession>
    <name>A0A6E8VFD2_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Guanylate kinase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MAGUK family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VFD2"/>
    <dbReference type="EnsemblMetazoa" id="ACON002711-RA">
        <property type="protein sequence ID" value="ACON002711-PA"/>
        <property type="gene ID" value="ACON002711"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000007"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002711"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032683"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005911">
        <property type="term" value="C:cell-cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00071">
        <property type="entry name" value="GMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06798">
        <property type="entry name" value="PDZ_MPP5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12036">
        <property type="entry name" value="SH3_MPP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.63.10:FF:000002">
        <property type="entry name" value="Guanylate kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000088">
        <property type="entry name" value="MAGUK p55 subfamily member 5"/>
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        <property type="entry name" value="MAGUK p55 subfamily member 5"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
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        <property type="entry name" value="Guanylate_kin-like_dom"/>
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        <property type="entry name" value="Guanylate_kinase_CS"/>
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        <property type="entry name" value="L27_C"/>
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        <property type="entry name" value="L27_dom_sf"/>
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        <property type="entry name" value="MAGUK"/>
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        <property type="entry name" value="MPP5_SH3"/>
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        <property type="entry name" value="P-loop_NTPase"/>
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        <property type="entry name" value="PDZ"/>
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        <property type="entry name" value="PDZ_sf"/>
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        <property type="entry name" value="SH3-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
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    <dbReference type="PANTHER" id="PTHR23122">
        <property type="entry name" value="MEMBRANE-ASSOCIATED GUANYLATE KINASE MAGUK"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
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    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
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        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00072">
        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101288">
        <property type="entry name" value="L27 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00856">
        <property type="entry name" value="GUANYLATE_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50052">
        <property type="entry name" value="GUANYLATE_KINASE_2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PDZ"/>
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    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="L27" evidence="8">
        <location>
            <begin position="155"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="7">
        <location>
            <begin position="300"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="5">
        <location>
            <begin position="392"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate kinase-like" evidence="6">
        <location>
            <begin position="542"/>
            <end position="722"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002711-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="738" mass="81124" checksum="A03D26703F74B9FF" modified="2020-08-12" version="1">MLDRAQQAGPAVKGTNGGVTLAPSNLLYAQRQEQLRRRREDEERIAAQNDFLRNSLRGSQRLRALQDNPIEKPPVGVDNEAYADDEVVAVAAEKIIGYGELVAALQRLQGHLNKHGLAALAGRVTAAQSLLLGPGIARALAVRTAVLERRRPKIPNPICPNAQALAKDCVESLAQSRSQTAIELCDLLSTYEMEGLLLAHDRIASTTDRSPIASYGGSPITVPVPLGTASNNTTSNNTTTSNNSINNNTLTSAVKPATVMPSIPNNASLVNNNNNNIAKREPMSVPLGVLRDGSQDHIKIIQIEKSSEPLGATIRNEGEAVVIGRVVRGGAAEKSGLLHEGDEILEVNGIEMRGKSVNDVCALLGSMTGTLTFLVVPAGVPQILPGLGMRDPPVLHVRAHFDYDPEDDLYIPCRELGISFQKGDVLHVISRDDPNWWQAYREGEEDQTLAGLIPSQSFQHQRESMKLAIAGEVGLRTRKDINGKAGGGSTLLCARKGRKKKKKASSEHGYPLYATATADDPDPEEILTYEEVALYYPRASHKRPIVLIGPPNIGRHELRQRLMADSERFAAAIPHTSRAQREGEVPGQDYHFISRQQFESDILARKFVEHGEYEKAYYGTSLEAIRAVVASGKICVLNLHPQSLKLLRSSDLKPYTVLVAPPSLEKLRQKRIRTGEPYKEEELKEIIATARDMEARWGHLFDMIIINNDTERAYHQLLAEINSLEREPQWVPSSWLHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VFD3</accession>
    <name>A0A6E8VFD3_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Guanylate kinase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MAGUK family.</text>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON002711-RF">
        <property type="protein sequence ID" value="ACON002711-PF"/>
        <property type="gene ID" value="ACON002711"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000007"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002711"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032683"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030054">
        <property type="term" value="C:cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00071">
        <property type="entry name" value="GMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06798">
        <property type="entry name" value="PDZ_MPP5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12036">
        <property type="entry name" value="SH3_MPP5"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MAGUK p55 subfamily member 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000469">
        <property type="entry name" value="MAGUK p55 subfamily member 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008144">
        <property type="entry name" value="Guanylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020590">
        <property type="entry name" value="Guanylate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014775">
        <property type="entry name" value="L27_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004172">
        <property type="entry name" value="L27_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050716">
        <property type="entry name" value="MAGUK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035601">
        <property type="entry name" value="MPP5_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23122">
        <property type="entry name" value="MEMBRANE-ASSOCIATED GUANYLATE KINASE MAGUK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02828">
        <property type="entry name" value="L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00072">
        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00856">
        <property type="entry name" value="GUANYLATE_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50052">
        <property type="entry name" value="GUANYLATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51022">
        <property type="entry name" value="L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="L27" evidence="9">
        <location>
            <begin position="702"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="8">
        <location>
            <begin position="847"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="6">
        <location>
            <begin position="939"/>
            <end position="1010"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate kinase-like" evidence="7">
        <location>
            <begin position="1089"/>
            <end position="1269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="234"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="381"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="479"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="237"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="265"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="385"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="492"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="522"/>
            <end position="534"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002711-PF"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1285" mass="138874" checksum="E7FFA60C79C429F1" modified="2020-08-12" version="1">MQQRTTTPAAGPPPPTASSSGKQVVELNGYVIILVESRDGKIKLYGSPADKDNLEVADEILDVNERKLEDSPRAEVIKHIHECIQSCMIKLRVKRRSDSRLAGELGNAVQDAFVIAVEQQARERLQRLSALKRITPVDMSQLSIKASISPTSHLIALNQQSQAKGGGTQDLSFLKEASPIYVTSLASNSVTGTNTSTTVTAGVKTTFPAGANNAINNNTLNNNNNSTVGGGGVLMQNATNNNGTTNLSSTTNTTGSSSSSGNASRLQQPGTTGMQVNVVTGQPGTGALVAVAASKGLSALDQPIPGNNNNGSGSTLGGGAALAGTSAYHSLHQHSLVNNNNLNSSNIGKPQGLDATANSYYSNLTNTFLANGHGSKRELATLSEGELEPPDEPQYESSGNISRGGTEVLLGDQNLRQENRPRRRSGSSIVVLGGDDTLKPSIPIDDYQEDLEMYNMLAANQDNGPHREMAVDVPESFIARNKTPPRYPPPRSTVTAASAAASTPATPASTQLNGSSKPVPPPRDHLRIEKDGRLTNRAPVPAPQVPDRKIVPNASSQHQHIGQVLEPTPDQLDSIKKFQEQLRRRREDEERIAAQNDFLRNSLRGSQRLRALQDNPIEKPPVGVDNEAYADDEVVAVAAEKIIGYGELVAALQRLQGHLNKHGLAALAGRVTAAQSLLLGPGIARALAVRTAVLERRRPKIPNPICPNAQALAKDCVESLAQSRSQTAIELCDLLSTYEMEGLLLAHDRIASTTDRSPIASYGGSPITVPVPLGTASNNTTSNNTTTSNNSINNNTLTSAVKPATVMPSIPNNASLVNNNNNNIAKREPMSVPLGVLRDGSQDHIKIIQIEKSSEPLGATIRNEGEAVVIGRVVRGGAAEKSGLLHEGDEILEVNGIEMRGKSVNDVCALLGSMTGTLTFLVVPAGVPQILPGLGMRDPPVLHVRAHFDYDPEDDLYIPCRELGISFQKGDVLHVISRDDPNWWQAYREGEEDQTLAGLIPSQSFQHQRESMKLAIAGEVGLRTRKDINGKAGGGSTLLCARKGRKKKKKASSEHGYPLYATATADDPDPEEILTYEEVALYYPRASHKRPIVLIGPPNIGRHELRQRLMADSERFAAAIPHTSRAQREGEVPGQDYHFISRQQFESDILARKFVEHGEYEKAYYGTSLEAIRAVVASGKICVLNLHPQSLKLLRSSDLKPYTVLVAPPSLEKLRQKRIRTGEPYKEEELKEIIATARDMEARWGHLFDMIIINNDTERAYHQLLAEINSLEREPQWVPSSWLHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VFD6</accession>
    <name>A0A6E8VFD6_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Isocitrate dehydrogenase [NAD] subunit, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">Ngousso</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="10">Heterooligomer of subunits alpha, beta, and gamma in the apparent ratio of 2:1:1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040221951.1">
        <property type="nucleotide sequence ID" value="XM_040366017.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VFD6"/>
    <dbReference type="EnsemblMetazoa" id="ACOM036454-RA">
        <property type="protein sequence ID" value="ACOM036454-PA"/>
        <property type="gene ID" value="ACOM036454"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON002728-RA">
        <property type="protein sequence ID" value="ACON002728-PA"/>
        <property type="gene ID" value="ACON002728"/>
    </dbReference>
    <dbReference type="GeneID" id="120949048"/>
    <dbReference type="CTD" id="3419"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007920"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002728"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037500"/>
    <dbReference type="Proteomes" id="UP000075882">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004449">
        <property type="term" value="F:isocitrate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006102">
        <property type="term" value="P:isocitrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.718.10:FF:000003">
        <property type="entry name" value="Isocitrate dehydrogenase [NAD] subunit, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.718.10">
        <property type="entry name" value="Isopropylmalate Dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019818">
        <property type="entry name" value="IsoCit/isopropylmalate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004434">
        <property type="entry name" value="Isocitrate_DH_NAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00175">
        <property type="entry name" value="mito_nad_idh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835">
        <property type="entry name" value="DECARBOXYLATING DEHYDROGENASES-ISOCITRATE, ISOPROPYLMALATE, TARTRATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835:SF34">
        <property type="entry name" value="ISOCITRATE DEHYDROGENASE [NAD] SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53659">
        <property type="entry name" value="Isocitrate/Isopropylmalate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00470">
        <property type="entry name" value="IDH_IMDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0520">NAD</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4 11" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Isopropylmalate dehydrogenase-like" evidence="12">
        <location>
            <begin position="25"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00065927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002728-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="354" mass="38846" checksum="549A75823051AAD8" modified="2020-08-12" version="1">MAARLIKKITVAPFGARTFASGTRKVTLIPGDGIGPEISAAVQKIFAVANVPIEWETVDVTPVRNPDGKFGIPQGAIDSVNRNKVGLKGPLMTPVGKGHRSLNLALRKEFNLYANVRPCRSLEGYKTLYDNVDVVTIRENTEGEYSGIEHEIVDGVVQSIKLITEEASNRVAEYAFKYAKDNNRKKVTVVHKANIMRMSDGLFLRCCRDMAQKYPEIKFEERYLDTVCLNMVQDPRKYDVLVMPNLYGDILSDMCAGLVGGLGLTPSGNMGLNGALFESVHGTAPDIAGKDLANPTALLLSAVMMLRHMELNQHADKIQSACFETIKEAKYLTGDLGGKAKCSEYTNAICDRIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VFG2</accession>
    <name>A0A6E8VFG2_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Sodium/potassium-transporting ATPase subunit alpha</fullName>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="22">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.</text>
    </comment>
    <comment type="subunit">
        <text evidence="19">The sodium/potassium-transporting ATPase is composed of a catalytic alpha subunit, an auxiliary non-catalytic beta subunit and an additional regulatory subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 20">Cell membrane</location>
            <topology evidence="1 20">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 20">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040225212.1">
        <property type="nucleotide sequence ID" value="XM_040369278.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040225213.1">
        <property type="nucleotide sequence ID" value="XM_040369279.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040225214.1">
        <property type="nucleotide sequence ID" value="XM_040369280.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_049462750.1">
        <property type="nucleotide sequence ID" value="XM_049606793.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VFG2"/>
    <dbReference type="EnsemblMetazoa" id="ACON002858-RB">
        <property type="protein sequence ID" value="ACON002858-PB"/>
        <property type="gene ID" value="ACON002858"/>
    </dbReference>
    <dbReference type="GeneID" id="120950886"/>
    <dbReference type="CTD" id="48971"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006768"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002858"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039874"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090533">
        <property type="term" value="C:cation-transporting ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098797">
        <property type="term" value="C:plasma membrane protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005391">
        <property type="term" value="F:P-type sodium:potassium-exchanging transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030007">
        <property type="term" value="P:intracellular potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006883">
        <property type="term" value="P:intracellular sodium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036376">
        <property type="term" value="P:sodium ion export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02608">
        <property type="entry name" value="P-type_ATPase_Na-K_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000038">
        <property type="entry name" value="Sodium/potassium-transporting ATPase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000003">
        <property type="entry name" value="Sodium/potassium-transporting ATPase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000001">
        <property type="entry name" value="Sodium/potassium-transporting ATPase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000004">
        <property type="entry name" value="Sodium/potassium-transporting ATPase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000095">
        <property type="entry name" value="Sodium/potassium-transporting ATPase subunit alpha-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050510">
        <property type="entry name" value="Cation_transp_ATPase_P-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005775">
        <property type="entry name" value="P-type_ATPase_IIC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01106">
        <property type="entry name" value="ATPase-IIC_X-K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43294">
        <property type="entry name" value="SODIUM/POTASSIUM-TRANSPORTING ATPASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43294:SF13">
        <property type="entry name" value="SODIUM_POTASSIUM-TRANSPORTING ATPASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00121">
        <property type="entry name" value="NAKATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15 20" id="KW-0406">Ion transport</keyword>
    <keyword evidence="16 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11 20" id="KW-0630">Potassium</keyword>
    <keyword evidence="5 20" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0915">Sodium</keyword>
    <keyword evidence="17" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="7" id="KW-0740">Sodium/potassium transport</keyword>
    <keyword evidence="12" id="KW-1278">Translocase</keyword>
    <keyword evidence="8 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 20" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="73"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="107"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="268"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="298"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="826"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="891"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="929"/>
            <end position="950"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="962"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="21">
        <location>
            <begin position="19"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00037422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00038795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU362084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002858-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1000" mass="110951" checksum="31A47D91310A6F96" modified="2020-08-12" version="1">MPPKKKTDLDDLKQELDIDYHKITPEELYQRLQTHPENGLSHAKAKENLERDGPNALTPPKQTPEWVKFCKNLFGGFALLLWIGAILCFIAYSIQASTVEEPADDNLYLGIVLAAVVIVTGIFSYYQESKSSKIMESFKNMVPQFATVLREGEKLTLRAEDLVIGDVVEVKFGDRIPADIRIIEARNFKVDNSSLTGESEPQSRGPDFTHENPLETKNLAFFSTNAVEGTAKGVVISCGDFTVMGRIAGLASGLDTGETPIAKEIHHFIHLITGVAVFLGVTFFVIAFILGYHWLDAVIFLIGIIVANVPEGLLATVTVCLTLTAKRMASKNCLVKNLEAVETLGSTSTICSDKTGTLTQNRMTVAHMWFDNQIIEADTTEDQSGVQYDRTSPGFKALSRIASLCNRAEFKGGQDGVPILKKEVSGDASEAALLKCMELALGDVLSIRKRNKKVCEIPFNSTNKYQVSIHETEDPSDPRYLLVMKGAPERILERCSTIFINGKEKLMDEEMKEAFNNAYLELGGLGERVLGFCDFMLPSDKYPVGFKFNSDDVNFQVDNLRFVGLMSMIDPPRAAVPDAVAKCRSAGIKVIMVTGDHPITAKAIAKSVGIISEGNETVEDIAQRLNIPVSEVNPREAKAAVVHGSELRDLSSDQLDEILRYHTEIVFARTSPQQKLIIVEGCQRMGAIVAVTGDGVNDSPALKKADIGVAMGIAGSDVSKQAADMILLDDNFASIVTGVEEGRLIFDNLKKSIAYTLTSNIPEISPFLAFILCDIPLPLGTVTILCIDLGTDMVPAISLAYEAPESDIMKRRPRDPYRDNLVNRRLISMAYGQIGMIQAAAGFFVYFVIMAENGFLPLKLFGLRKSWDSKAINDLTDSYGQEWTYRDRKTLEFTCHTAFFVSIVVVQWADLIICKTRRNSIFHQGMRNWALNFGLVFETVLAAILSYTPGMDKGLRMYPLKFVWWLPALPFSLSIFVYDEIRRFYLRRNPGGWLEQETYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VFG6</accession>
    <name>A0A6E8VFG6_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Cysteine proteinase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the cystatin family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C1 family.</text>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON002879-RA">
        <property type="protein sequence ID" value="ACON002879-PA"/>
        <property type="gene ID" value="ACON002879"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005239"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002879"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030656"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004869">
        <property type="term" value="F:cysteine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00042">
        <property type="entry name" value="CY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02248">
        <property type="entry name" value="Peptidase_C1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000130">
        <property type="entry name" value="Cysteine proteinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.10">
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000010">
        <property type="entry name" value="Cystatin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046350">
        <property type="entry name" value="Cystatin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025661">
        <property type="entry name" value="Pept_asp_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025660">
        <property type="entry name" value="Pept_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039417">
        <property type="entry name" value="Peptidase_C1A_papain-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018073">
        <property type="entry name" value="Prot_inh_cystat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013201">
        <property type="entry name" value="Prot_inhib_I29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08246">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00705">
        <property type="entry name" value="PAPAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00848">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54403">
        <property type="entry name" value="Cystatin/monellin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00287">
        <property type="entry name" value="CYSTATIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00640">
        <property type="entry name" value="THIOL_PROTEASE_ASN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00639">
        <property type="entry name" value="THIOL_PROTEASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026274164" description="Cysteine proteinase" evidence="11">
        <location>
            <begin position="25"/>
            <end position="1807"/>
        </location>
    </feature>
    <feature type="domain" description="Cathepsin propeptide inhibitor" evidence="13">
        <location>
            <begin position="1497"/>
            <end position="1554"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="12">
        <location>
            <begin position="1587"/>
            <end position="1805"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="24"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="205"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="285"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="389"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="744"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1345"/>
            <end position="1367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1444"/>
            <end position="1478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="219"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="294"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="413"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="436"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="518"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="568"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="598"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="757"/>
            <end position="768"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002879-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1807" mass="199219" checksum="173F9EDF0488530A" modified="2020-08-12" version="1">MRPILQLGLLVVVVVAAAATVTRAEEQPNPVTEAEVNSNGPTEEPEEEVQDAPSTVNGSSEGGIVAIHVDAVGGKEQLRPTLRGLSYLATSFLPRDYKFVEIVSATREVVAGVRYELLASAEDETTGQRHLCQLVILEKPWITNEYGEKYRTLEYTNCTLPSTHPAMAETATRLNEAFDANLRQNEELTPQRIEELEQQIVVEKGDVSTTPETQDAPVTGSSSSLPDAATTTEGEEGLSEAAKSAIDELFSFASAGSLQSARSNQGAAAAAAGTEVVEEPMVRKVVSKERSEPEPVTTEPTTTSSTTIAPLDQQVQSTFDEVFKAHQEIQKALDDIIANGGGRDVQLKYRPVLDSLLQRVKASIDTYYRTTNGGDANVDTAAFVVRDNTNVAPNGGRPPARIVPPSASRSEDQSSQESDEGQQQQRQQLPDVNLQPPRANAPTAAATYGADSDEDENDQQIVTKEQLAQELNNEEGLQRVKKHAEGKPEEDTAVDSTTVSDADTAAFGDTTVPAVKNSTPETTTPLVVPSSTSTAANRVATSSSSSSKESDESKKPKKNKMKKTTSDSSDDTDEEENDTMGVKLSKEQLTRQLNADEGGQRRDKRDFSGGFADGSFGGFSGSSFGGGSYSDGGYYGGGGGYLGGGSYHGGYYIGGYNPYRRKYYRPSPYYYGGYRGKRDVAAVDNFPADLNEQGEEVVKQAELNEHSANLKPPKIERVTMAFGDSNEEEEDVVLTKEQLAQQLNADDGAFRRDKRGYSSSFDHSSSQQHHGHGYYGGYNVRPSYYNQPPAAYYGYYRGKRNTNPPDSNLVNLDDPVQEASMRAIATEAIQRLDQMDSDPYQRMLLEVLDARRVAQRSDKSRTTYVLSVLTANSRCAEVEEEKESKVTERPCRERLMPDTTKQCTLEANNLGGRLVGGATPVDVKEPTHIERVRLGLVGYESGKHSNFEILFGTVQVVAGTIHRYKIALKDDDQKVYSTCDVKVFTPLPSAANGSKPDYDFDCQDEEEAAAAGAHRARRDVVAAPPKKLNKVPKAGAAQELTPEEYAKEEHQTRIRTGLQQQSALVDGSGNERKVKVVGATVQLVAGKSYTYRLSFPDDELKRVCKLTVWEKPWLKEKAPQEAFKASFECPDGAKRTKRATVGVDCLGCASPLAANEYGNSEHQERIDKILSFHGLTRGNSLKVINATSQVVAGMKYVYFIQHNNAVCKLTSWERVWLAQSHPEDAYKYTYDCEKEAGPAKVRTRRSVVPGGSRSLSQDELAAAEHLERVDKILVSSGGSKESSNARIVSGTVQIVSGKLYKYAVEFDVDGSSKLCKLSSWERPWLVKKDPTEAYKYTVSCPSEKAAGRQRRHAKKAGSSNELTAEELKDKSHVERIRAGMVSYNSERSKAYNEFEILAGSTQQVAGSLYKYTFRVTSESDIVCKISIWERVWLESQDQRKYNVKCTGDDDTEQQPDIKSPQQPDAPAPTPVTTAPAVKRRSVRSLKIDDDAHVRRMFDKFRHHHRRQYASSMEHEMRFNIFRNNLFKIEQLNKFERGTAKYGVTKFADMTVAEYRAHTGLVVPKHDRANHVGNRVASEEDVAGVGDLPRSFDWRDHGAVTEVKNQGSCGSCWAFSAVGNVEGLHQIKTKKLESYSEQELIDCDKVDNGCGGGYMDDAFKAIEQLGGLELENDYPYEAKAQKSCHFNRSLSHVQVKGAVDMPKNETYIAQYLIKNGPIAIGLNANAMQFYRGGISHPWHPLCNHKSIDHGVLIVGYGIKEYPMFNKTLPYWIIKNSWGPRWGEQGYYRIYRGDNSCGVSEMASSAILA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VFG8</accession>
    <name>A0A6E8VFG8_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VFG8"/>
    <dbReference type="EnsemblMetazoa" id="ACON002888-RA">
        <property type="protein sequence ID" value="ACON002888-PA"/>
        <property type="gene ID" value="ACON002888"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005269"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002888"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032240"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004989">
        <property type="term" value="F:octopamine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071880">
        <property type="term" value="P:adenylate cyclase-activating adrenergic receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043410">
        <property type="term" value="P:positive regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15066">
        <property type="entry name" value="7tmA_DmOct-betaAR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248">
        <property type="entry name" value="ADRENERGIC RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248:SF66">
        <property type="entry name" value="OCTOPAMINE RECEPTOR BETA-3R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 10" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 10" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 10 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="119"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="151"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="190"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="231"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="279"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="393"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="428"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="131"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="61"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="70"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002888-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="482" mass="53132" checksum="61D242BB870F7E0C" modified="2020-08-12" version="1">MFTSRSVPPTAQPEVNALLGAATQPITLPGALATVASALASAGSATLTSLAMAPTSGPEVDGAAGGWNGTGDATSGAAGDHQPVGPGSNAVNLTVPTASTIVEPADRLVELTVLCLKSLIFGSIIIGAVLGNALVIISVHKNRKLRVITNYFVVSLAMADMLVALCAMTFNASVELSGRWLFGSFMCDVWNSLDVYFSTASILHLCCISVDRYFAIVRPLEYPLYMTQRTVFFMLANVWVLPALISFTPIFLGWYTTAEHLAALKINPDLCIFVVNKSYAIISSSISFWIPGIVMVTMYYRIYKEAVRQRKALSRTSSNILLNSVQINNASNTLHANHHHHNHHHQHLHHRNHHLRASDCDLGIDIKDIQHDTHSELSDLDGWRAEHKAARTLGIIMGVFLLCWLPFFLWYVITTLCGEEACPCPDVVITVLFWIGYFNSTLNPLIYAYFNRDFREAFRNTLQSLLPCFVRRSPYGHSVYYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VGZ2</accession>
    <name>A0A6E8VGZ2_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="5">B30.2/SPRY domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_040225553.2">
        <property type="nucleotide sequence ID" value="XM_040369619.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VGZ2"/>
    <dbReference type="EnsemblMetazoa" id="ACON003705-RA">
        <property type="protein sequence ID" value="ACON003705-PA"/>
        <property type="gene ID" value="ACON003705"/>
    </dbReference>
    <dbReference type="GeneID" id="120951093"/>
    <dbReference type="CTD" id="42936"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009508"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003705"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037269"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048188">
        <property type="term" value="C:Set1C/COMPASS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12872">
        <property type="entry name" value="SPRY_Ash2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037353">
        <property type="entry name" value="ASH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10598">
        <property type="entry name" value="SET1/ASH2 HISTONE METHYLTRANSFERASE COMPLEX SUBUNIT ASH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10598:SF0">
        <property type="entry name" value="SET1_ASH2 HISTONE METHYLTRANSFERASE COMPLEX SUBUNIT ASH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B30.2/SPRY" evidence="4">
        <location>
            <begin position="79"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003705-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="347" mass="39489" checksum="60FBCE842ED0E2DF" modified="2020-08-12" version="1">MEETTQNFSKSRQQKRKLPNSEQSGALGKKSRLGTDVGALVKLPAHGYPLEHPFNKDGYRYILAEPDPHAPFRQEFDESADWAGKPIPGWLYRVLSPNAVLIALHDRAPQLKVSEDRLSITGEKGYCMARASHYVTKGCWYWEATVEDMPDGSACRLGWGQEYANLQAPLGYDKFGYSWRSRKGTKFHESHGKHYSAGYGEGDTLGFLITLPSDNQANQASNTFKDRPLVKFKSHLYYEDKDRVNETLKALKVQLGSKIHYFKNGVCQGEAFVDVYKGAYYPAISLHKNVTISVNFGPKFKHPEVLKEFKAQSMHERVEEMICEQTMADMMYFTENDGKLRLDTYSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VGZ6</accession>
    <name>A0A6E8VGZ6_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">V-type proton ATPase subunit a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Essential component of the vacuolar proton pump (V-ATPase), a multimeric enzyme that catalyzes the translocation of protons across the membranes. Required for assembly and activity of the V-ATPase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the V-ATPase 116 kDa subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VGZ6"/>
    <dbReference type="EnsemblMetazoa" id="ACON003711-RB">
        <property type="protein sequence ID" value="ACON003711-PB"/>
        <property type="gene ID" value="ACON003711"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON003711-RC">
        <property type="protein sequence ID" value="ACON003711-PC"/>
        <property type="gene ID" value="ACON003711"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007046"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003711"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037888"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000220">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051117">
        <property type="term" value="F:ATPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002490">
        <property type="entry name" value="V-ATPase_116kDa_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026028">
        <property type="entry name" value="V-type_ATPase_116kDa_su_euka"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629:SF63">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629">
        <property type="entry name" value="VACUOLAR PROTON ATPASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01496">
        <property type="entry name" value="V_ATPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001293">
        <property type="entry name" value="ATP6V0A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 9" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="402"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="451"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="540"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="573"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="605"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="645"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="766"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="51"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003711-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="838" mass="95326" checksum="C5DCEB6AFCA66F1B" modified="2020-08-12" version="1">MGSLFRSEEMSLCQLFLQSEAAYACVSELGELGLVQFRDLNPDVNAFQRKFVNEVRRCDEMERKLRYLEKEIKKDGIPMLDTGESPEAPQPREMIDLEATFEKLENELREVNQNAEALKRNYLELTELKHILRKTQVFFDEMADSHREEEQVNLLGEEGIRAGGAGAQGQNLKLGFVAGVILRERLPAFERMLWRACRGNVFLRQAMIEDPLEDPSNGDKVYKSVFIIFFQGDQLKTRVKKICEGFRATLYPCPEAPTDRREMAMGVMTRIEDLHTVLGQTQDHRHRVLVAAAKNLKNWFVKVRKIKAIYHTLNLFNLDVTQKCLIAECWVPLLDFETIQIALRRGTERSGSSVPPILNRMETFEDPPTYNRTNKFTHAFQALINAYGVASYREMNPAPYTIITFPFLFAVMFGDLGHGTIMALFGLWMVLKEKPLAAKKSDNEIWNIFFGGRYIIFLMGVFSMYTGFVYNDIFSKSLNVFGSAWSTNYNTSTVMSNKALQLNPKGMDYAQTPYPFGLDPVWQVAPLNKIIFQNAYKMKISIIFGVIHMLFGVFVGLFNHRYFKNKMAIYCEFIPQVIFLVFLFFYMTLLMFIKWVKYSASATDVVYSEGCAPSILITFINMVLFKAPDKNTGDCLPYMFAGQSGLQKFLVVVALLCVPWMLLAKPILIMRGRKEAAVSVCIETLDDSGTQAGQQPAQQQGGGHGHDNEEMSEIFIHQGIHTIEYVLGSVSHTASYLRLWALSLAHAQLAEVLWNMVLQNGLKQDGWIGGIALWAVFAFWAVLTVGILVLMEGLSAFLHTLRLHWVEFQSKFYAGLGYAFQPFSFEVILESSSSSSED</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VGZ8</accession>
    <name>A0A6E8VGZ8_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">BK channel</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Maxi K channel</fullName>
        </alternativeName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="23">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the potassium channel family. Calcium-activated (TC 1.A.1.3) subfamily. Slo sub-subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VGZ8"/>
    <dbReference type="EnsemblMetazoa" id="ACON003709-RE">
        <property type="protein sequence ID" value="ACON003709-PE"/>
        <property type="gene ID" value="ACON003709"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_014063"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003709"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033153"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034702">
        <property type="term" value="C:monoatomic ion channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060072">
        <property type="term" value="F:large conductance calcium-activated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050804">
        <property type="term" value="P:modulation of chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009410">
        <property type="term" value="P:response to xenobiotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000035">
        <property type="entry name" value="Calcium-activated potassium channel slowpoke"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000005">
        <property type="entry name" value="calcium-activated potassium channel subunit alpha-1 isoform X6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000015">
        <property type="entry name" value="Calcium-activated potassium channel subunit alpha-1 isoform X7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003929">
        <property type="entry name" value="K_chnl_BK_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047871">
        <property type="entry name" value="K_chnl_Slo-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048735">
        <property type="entry name" value="Slowpoke-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10027">
        <property type="entry name" value="CALCIUM-ACTIVATED POTASSIUM CHANNEL ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10027:SF33">
        <property type="entry name" value="CALCIUM-ACTIVATED POTASSIUM CHANNEL SUBUNIT ALPHA-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03493">
        <property type="entry name" value="BK_channel_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22614">
        <property type="entry name" value="Slo-like_RCK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21014">
        <property type="entry name" value="Slowpoke_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01449">
        <property type="entry name" value="BKCHANNELA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00169">
        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16" id="KW-0407">Ion channel</keyword>
    <keyword evidence="14" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-0630">Potassium</keyword>
    <keyword evidence="8" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="11" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="29"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="109"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="148"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="172"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="231"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="267"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="293"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="22">
        <location>
            <begin position="337"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="22">
        <location>
            <begin position="784"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="678"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1123"/>
            <end position="1154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="678"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="699"/>
            <end position="730"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00029579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00031999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00060897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003709-PE"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1154" mass="129149" checksum="50E9A20ED122C728" modified="2020-08-12" version="1">MSGCDEEQSVLTTELPPTDLDDCLRVRKWWCFLLSSIFTFLAGLLAVLLWRAFAFLCCRKEPELAPNDPKQKEQKASRQGKQDFEGTFMTEAKDWAGELISGQTTTGRILVVLVFILSIASLIIYFIDASNEEVERCQKWSNNITQQIDLAFNIFFMVYFFIRFIAASDKLWFMLEMYSFVDYFTIPPSFVSIYLDRTWIGLRFLRALRLMTVPDILQYLNILKTSSSIRLAQLVSIFISVWLTAAGIIHLLENSGDPLEFNNPQQLSYWTCVYFLIVTMSTVGYGDVYCETVLGRTFLVFFLLVGLAVFASWIPEITELAAQRNKYGGKYTKDVRRRHIVVCGHITYESVSHFLKDFLHEDREDVDVEVVFLHRKPPDLELEGLFKRHFTTVEFFQGTIMSPIDLQRVKVHEADACLVLANKYCQDPDAEDAANIMRVISIKNYSDDIRVIIQLMQYHNKAYLLNIPSWDWKQGDDVICLAELKLGFIAQSCLAPGFSTMMANLFAMRSFKTSPDTQAWQNDYLQGTGCEMYTETLSPSFTGMTFPQASELCFTKLKLLLLAIEIKGGEEGNDSKISINPRGAKILANTQGFFIAQSADEVKRAWFYCKACHDDIKDETLIKKCKCKNFTLRKTTAILLGCDITRDREDINLSCNIDVYRSNGALLLINRNRRSNVSMTGSNQMLNSTKQVNKVKPNVNRQPPDNLISPSTYNRPTSRGSGSGAQSQNGVTLQAGIADDQSKDFDFEKTEMKYDSTGMFHWSPAKSLEECILDRNQAAMTVLNGHVVVCLFADPDSPLIGLRNLVMPLRASNFHYHELKHVVIVGSVEYIRREWKMLQNLPKISVLNGSPLSRADLRAVNVNLCDMCCILSAKVPSNDDPTLADKEAILASLNIKAMTFDDTIGVLSQRGSEQDNLTPVGSPIVLQRRGSVYGANVPMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSTTYFNQNALTLIRSLITGGATPELELILAEGAGLRGGYSTPESLSNRDRCRVGQISLYDGPLAQFGEAGKYGDLFVAALKSYGMLCIGLYRFRDTSSSCDASSKRYVITNPPDDFSLLPTDQVFVLMQFDPGLEYKPATVRGPSGGGRNQNSQAAGVGGGGSNKDDNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VGZ9</accession>
    <name>A0A6E8VGZ9_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">BK channel</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Maxi K channel</fullName>
        </alternativeName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="23">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the potassium channel family. Calcium-activated (TC 1.A.1.3) subfamily. Slo sub-subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VGZ9"/>
    <dbReference type="EnsemblMetazoa" id="ACON003709-RB">
        <property type="protein sequence ID" value="ACON003709-PB"/>
        <property type="gene ID" value="ACON003709"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_014063"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003709"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033153"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034702">
        <property type="term" value="C:monoatomic ion channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060072">
        <property type="term" value="F:large conductance calcium-activated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050804">
        <property type="term" value="P:modulation of chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009410">
        <property type="term" value="P:response to xenobiotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000035">
        <property type="entry name" value="Calcium-activated potassium channel slowpoke"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000005">
        <property type="entry name" value="calcium-activated potassium channel subunit alpha-1 isoform X6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000015">
        <property type="entry name" value="Calcium-activated potassium channel subunit alpha-1 isoform X7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003929">
        <property type="entry name" value="K_chnl_BK_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047871">
        <property type="entry name" value="K_chnl_Slo-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048735">
        <property type="entry name" value="Slowpoke-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10027">
        <property type="entry name" value="CALCIUM-ACTIVATED POTASSIUM CHANNEL ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10027:SF33">
        <property type="entry name" value="CALCIUM-ACTIVATED POTASSIUM CHANNEL SUBUNIT ALPHA-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03493">
        <property type="entry name" value="BK_channel_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22614">
        <property type="entry name" value="Slo-like_RCK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21014">
        <property type="entry name" value="Slowpoke_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01449">
        <property type="entry name" value="BKCHANNELA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00169">
        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16" id="KW-0407">Ion channel</keyword>
    <keyword evidence="14" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-0630">Potassium</keyword>
    <keyword evidence="8" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="11" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="29"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="109"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="148"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="172"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="231"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="267"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="293"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="22">
        <location>
            <begin position="337"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="22">
        <location>
            <begin position="784"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="678"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1123"/>
            <end position="1154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="678"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="699"/>
            <end position="730"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00029579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00031999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00060897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003709-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1154" mass="129169" checksum="66584AF9BFFB94F3" modified="2020-08-12" version="1">MSGCDEEQSVLTTELPPTDLDDCLRVRKWWCFLLSSIFTFLAGLLAVLLWRAFAFLCCRKEPELAPNDPKQKEQKASRQGKQDFEGTFMTEAKDWAGELISGQTTTGRILVVLVFILSIASLIIYFIDASNEEVERCQKWSNNITQQIDLAFNIFFMVYFFIRFIAASDKLWFMLEMYSFVDYFTIPPSFVSIYLDRTWIGLRFLRALRLMTVPDILQYLNILKTSSSIRLAQLVSIFISVWLTAAGIIHLLENSGDPLEFNNPQQLSYWTCVYFLIVTMSTVGYGDVYCETVLGRTFLVFFLLVGLAVFASWIPEITELAAQRNKYGGKYTKDVRRRHIVVCGHITYESVSHFLKDFLHEDREDVDVEVVFLHRKEPDLELEGLLKRHYTTVEFFQGTMMNAVDLERVKVHEADACLVLANKYCQDPDAEDAANIMRVISIKNYSDDIRVIIQLMQYHNKAYLLNIPSWDWKQGDDVICLAELKLGFIAQSCLAPGFSTMMANLFAMRSFKTSPDTQAWQNDYLQGTGCEMYTETLSPSFTGMTFPQASELCFTKLKLLLLAIEIKGGEEGNDSKISINPRGAKILANTQGFFIAQSADEVKRAWFYCKACHDDIKDETLIKKCKCKNFTLRKTTAILLGCDITRDREDINLSCNIDVYRSNGALLLINRNRRSNVSMTGSNQMLNSTKQVNKVKPNVNRQPPDNLISPSTYNRPTSRGSGSGAQSQNGVTLQAGIADDQSKDFDFEKTEMKYDSTGMFHWSPAKSLEECILDRNQAAMTVLNGHVVVCLFADPDSPLIGLRNLVMPLRASNFHYHELKHVVIVGSVEYIRREWKMLQNLPKISVLNGSPLSRADLRAVNVNLCDMCCILSAKVPSNDDPTLADKEAILASLNIKAMTFDDTIGVLSQRGSEQDNLTPVGSPIVLQRRGSVYGANVPMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSTTYFNQNALTLIRSLITGGATPELELILAEGAGLRGGYSTPESLSNRDRCRVGQISLYDGPLAQFGEAGKYGDLFVAALKSYGMLCIGLYRFRDTSSSCDASSKRYVITNPPDDFSLLPTDQVFVLMQFDPGLEYKPATVRGPSGGGRNQNSQAAGVGGGGSNKDDNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VHJ0</accession>
    <name>A0A6E8VHJ0_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">uridine/cytidine kinase</fullName>
            <ecNumber evidence="4">2.7.1.48</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>cytidine + ATP = CMP + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24674"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17562"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.48"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>uridine + ATP = UMP + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16825"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16704"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.48"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Pyrimidine metabolism; CTP biosynthesis via salvage pathway; CTP from cytidine: step 1/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; UMP biosynthesis via salvage pathway; UMP from uridine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the uridine kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.48" evidence="4"/>
    <dbReference type="RefSeq" id="XP_040222746.1">
        <property type="nucleotide sequence ID" value="XM_040366812.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VHJ0"/>
    <dbReference type="EnsemblMetazoa" id="ACON003874-RA">
        <property type="protein sequence ID" value="ACON003874-PA"/>
        <property type="gene ID" value="ACON003874"/>
    </dbReference>
    <dbReference type="GeneID" id="120949484"/>
    <dbReference type="CTD" id="42894"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007515"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003874"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033523"/>
    <dbReference type="UniPathway" id="UPA00574">
        <property type="reaction ID" value="UER00637"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00579">
        <property type="reaction ID" value="UER00640"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004849">
        <property type="term" value="F:uridine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044211">
        <property type="term" value="P:CTP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044206">
        <property type="term" value="P:UMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd02023">
        <property type="entry name" value="UMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000297">
        <property type="entry name" value="Uridine-cytidine kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006083">
        <property type="entry name" value="PRK/URK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000764">
        <property type="entry name" value="Uridine_kinase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004018">
        <property type="entry name" value="PRK05480.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10285">
        <property type="entry name" value="URIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00485">
        <property type="entry name" value="PRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00988">
        <property type="entry name" value="URIDINKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribulokinase/uridine kinase" evidence="11">
        <location>
            <begin position="25"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003874-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="244" mass="27288" checksum="D0A0CBAAF778CFFF" modified="2023-06-28" version="2">MGDGECLNGARLNGGAADGVKTPFLIGVAGGTASGKSTVCKRIMEQLGQADMDHTQRQVVTISQDSFYRELSDSEKARAERGLFNFDHPSAFNEDLMLQTLQDILHGKKVEISEYDYRRNAVCPEKKITIYPADVVLFEGILVFYFPAIRDLFHMKLFVDTDSDTRLARRVPRDINERGRDLDQVLNAYMNFVKPAFEEFCSPTKKFADVVIPRGADNTVAIDLIVHHINEILHPSGGLNQNGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VHJ1</accession>
    <name>A0A6E8VHJ1_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">BK channel</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Maxi K channel</fullName>
        </alternativeName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="23">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the potassium channel family. Calcium-activated (TC 1.A.1.3) subfamily. Slo sub-subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VHJ1"/>
    <dbReference type="EnsemblMetazoa" id="ACON003709-RA">
        <property type="protein sequence ID" value="ACON003709-PA"/>
        <property type="gene ID" value="ACON003709"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_014063"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003709"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033153"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034702">
        <property type="term" value="C:monoatomic ion channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060072">
        <property type="term" value="F:large conductance calcium-activated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050804">
        <property type="term" value="P:modulation of chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009410">
        <property type="term" value="P:response to xenobiotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000035">
        <property type="entry name" value="Calcium-activated potassium channel slowpoke"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000005">
        <property type="entry name" value="calcium-activated potassium channel subunit alpha-1 isoform X6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000015">
        <property type="entry name" value="Calcium-activated potassium channel subunit alpha-1 isoform X7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003929">
        <property type="entry name" value="K_chnl_BK_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047871">
        <property type="entry name" value="K_chnl_Slo-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048735">
        <property type="entry name" value="Slowpoke-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10027">
        <property type="entry name" value="CALCIUM-ACTIVATED POTASSIUM CHANNEL ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10027:SF33">
        <property type="entry name" value="CALCIUM-ACTIVATED POTASSIUM CHANNEL SUBUNIT ALPHA-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03493">
        <property type="entry name" value="BK_channel_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22614">
        <property type="entry name" value="Slo-like_RCK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21014">
        <property type="entry name" value="Slowpoke_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01449">
        <property type="entry name" value="BKCHANNELA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00169">
        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16" id="KW-0407">Ion channel</keyword>
    <keyword evidence="14" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-0630">Potassium</keyword>
    <keyword evidence="8" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="11" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="29"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="109"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="148"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="172"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="231"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="267"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="298"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="22">
        <location>
            <begin position="337"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="22">
        <location>
            <begin position="784"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="678"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1123"/>
            <end position="1154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="678"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="699"/>
            <end position="730"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00029579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00031999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00060897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003709-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1154" mass="129053" checksum="A38C7D25A7E2D8B3" modified="2020-08-12" version="1">MSGCDEEQSVLTTELPPTDLDDCLRVRKWWCFLLSSIFTFLAGLLAVLLWRAFAFLCCRKEPELAPNDPKQKEQKASRQGKQDFEGTFMTEAKDWAGELISGQTTTGRILVVLVFILSIASLIIYFIDASNEEVERCQKWSNNITQQIDLAFNIFFMVYFFIRFIAASDKLWFMLEMYSFVDYFTIPPSFVSIYLDRTWIGLRFLRALRLMTVPDILQYLNILKTSSSIRLAQLVSIFISVWLTAAGIIHLLENSGDPLEFNNPQQLSYWTCVYFLIVTMSTVGYGDVYCETVLGRTFLVFFLLVGLAIFASCIPEIIDLIGTRPKYGGTLKNERGRRHIVVCGHITYESVSHFLKDFLHEDREDVDVEVVFLHRKPPDLELEGLFKRHFTTVEFFQGTIMSPIDLQRVKVHEADACLVLANKYCQDPDAEDAANIMRVISIKNYSDDIRVIIQLMQYHNKAYLLNIPSWDWKQGDDVICLAELKLGFIAQSCLAPGFSTMMANLFAMRSFKTSPDMQVWTNDYLRGTGMEMYTETLSPSFIGMPFAQATELCFTKLKLLLLAIEIKGGEEGNDSKISINPRGAKILANTQGFFIAQSADEVKRAWFYCKACHDDIKDETLIKKCKCKNFTLRKTTAILLGCDITRDREDINLSCNIDVYRSNGALLLINRNRRSNVSMTGSNQMLNSTKQVNKVKPNVNRQPPDNLISPSTYNRPTSRGSGSGAQSQNGVTLQAGIADDQSKDFDFEKTEMKYDSTGMFHWSPAKSLEECILDRNQAAMTVLNGHVVVCLFADPDSPLIGLRNLVMPLRASNFHYHELKHVVIVGSVEYIRREWKMLQNLPKISVLNGSPLSRADLRAVNVNLCDMCCILSAKVPSNDDPTLADKEAILASLNIKAMTFDDTIGVLSQRGSEQDNLTPVGSPIVLQRRGSVYGANVPMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSTTYFNQNALTLIRSLITGGATPELELILAEGAGLRGGYSTPESLSNRDRCRVGQISLYDGPLAQFGEAGKYGDLFVAALKSYGMLCIGLYRFRDTSSSCDASSKRYVITNPPDDFSLLPTDQVFVLMQFDPGLEYKPATVRGPSGGGRNQNSQAAGVGGGGSNKDDNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VHJ6</accession>
    <name>A0A6E8VHJ6_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">BK channel</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Maxi K channel</fullName>
        </alternativeName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="23">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the potassium channel family. Calcium-activated (TC 1.A.1.3) subfamily. Slo sub-subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VHJ6"/>
    <dbReference type="EnsemblMetazoa" id="ACON003709-RF">
        <property type="protein sequence ID" value="ACON003709-PF"/>
        <property type="gene ID" value="ACON003709"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_014063"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003709"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033153"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034702">
        <property type="term" value="C:monoatomic ion channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060072">
        <property type="term" value="F:large conductance calcium-activated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050804">
        <property type="term" value="P:modulation of chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009410">
        <property type="term" value="P:response to xenobiotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000035">
        <property type="entry name" value="Calcium-activated potassium channel slowpoke"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000005">
        <property type="entry name" value="calcium-activated potassium channel subunit alpha-1 isoform X6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000015">
        <property type="entry name" value="Calcium-activated potassium channel subunit alpha-1 isoform X7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003929">
        <property type="entry name" value="K_chnl_BK_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047871">
        <property type="entry name" value="K_chnl_Slo-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048735">
        <property type="entry name" value="Slowpoke-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10027">
        <property type="entry name" value="CALCIUM-ACTIVATED POTASSIUM CHANNEL ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10027:SF33">
        <property type="entry name" value="CALCIUM-ACTIVATED POTASSIUM CHANNEL SUBUNIT ALPHA-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03493">
        <property type="entry name" value="BK_channel_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22614">
        <property type="entry name" value="Slo-like_RCK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21014">
        <property type="entry name" value="Slowpoke_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01449">
        <property type="entry name" value="BKCHANNELA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00169">
        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16" id="KW-0407">Ion channel</keyword>
    <keyword evidence="14" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-0630">Potassium</keyword>
    <keyword evidence="8" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="11" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="29"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="109"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="148"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="172"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="231"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="267"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="298"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="22">
        <location>
            <begin position="337"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="22">
        <location>
            <begin position="784"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="678"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1123"/>
            <end position="1154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="678"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="699"/>
            <end position="730"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00029579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00031999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00060897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003709-PF"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1154" mass="129073" checksum="953D95D2C93B8B68" modified="2020-08-12" version="1">MSGCDEEQSVLTTELPPTDLDDCLRVRKWWCFLLSSIFTFLAGLLAVLLWRAFAFLCCRKEPELAPNDPKQKEQKASRQGKQDFEGTFMTEAKDWAGELISGQTTTGRILVVLVFILSIASLIIYFIDASNEEVERCQKWSNNITQQIDLAFNIFFMVYFFIRFIAASDKLWFMLEMYSFVDYFTIPPSFVSIYLDRTWIGLRFLRALRLMTVPDILQYLNILKTSSSIRLAQLVSIFISVWLTAAGIIHLLENSGDPLEFNNPQQLSYWTCVYFLIVTMSTVGYGDVYCETVLGRTFLVFFLLVGLAIFASCIPEIIDLIGTRPKYGGTLKNERGRRHIVVCGHITYESVSHFLKDFLHEDREDVDVEVVFLHRKEPDLELEGLLKRHYTTVEFFQGTMMNAVDLERVKVHEADACLVLANKYCQDPDAEDAANIMRVISIKNYSDDIRVIIQLMQYHNKAYLLNIPSWDWKQGDDVICLAELKLGFIAQSCLAPGFSTMMANLFAMRSFKTSPDMQVWTNDYLRGTGMEMYTETLSPSFIGMPFAQATELCFTKLKLLLLAIEIKGGEEGNDSKISINPRGAKILANTQGFFIAQSADEVKRAWFYCKACHDDIKDETLIKKCKCKNFTLRKTTAILLGCDITRDREDINLSCNIDVYRSNGALLLINRNRRSNVSMTGSNQMLNSTKQVNKVKPNVNRQPPDNLISPSTYNRPTSRGSGSGAQSQNGVTLQAGIADDQSKDFDFEKTEMKYDSTGMFHWSPAKSLEECILDRNQAAMTVLNGHVVVCLFADPDSPLIGLRNLVMPLRASNFHYHELKHVVIVGSVEYIRREWKMLQNLPKISVLNGSPLSRADLRAVNVNLCDMCCILSAKVPSNDDPTLADKEAILASLNIKAMTFDDTIGVLSQRGSEQDNLTPVGSPIVLQRRGSVYGANVPMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSTTYFNQNALTLIRSLITGGATPELELILAEGAGLRGGYSTPESLSNRDRCRVGQISLYDGPLAQFGEAGKYGDLFVAALKSYGMLCIGLYRFRDTSSSCDASSKRYVITNPPDDFSLLPTDQVFVLMQFDPGLEYKPATVRGPSGGGRNQNSQAAGVGGGGSNKDDNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VHK5</accession>
    <name>A0A6E8VHK5_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="8">C-terminal binding protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VHK5"/>
    <dbReference type="EnsemblMetazoa" id="ACON003893-RB">
        <property type="protein sequence ID" value="ACON003893-PB"/>
        <property type="gene ID" value="ACON003893"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035290"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140297">
        <property type="term" value="F:DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001221">
        <property type="term" value="F:transcription coregulator binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05299">
        <property type="entry name" value="CtBP_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000012">
        <property type="entry name" value="C-terminal-binding protein 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043322">
        <property type="entry name" value="CtBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051638">
        <property type="entry name" value="CTBP_dehydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029753">
        <property type="entry name" value="D-isomer_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46029">
        <property type="entry name" value="C-TERMINAL-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46029:SF7">
        <property type="entry name" value="C-TERMINAL-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00671">
        <property type="entry name" value="D_2_HYDROXYACID_DH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase catalytic" evidence="6">
        <location>
            <begin position="34"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding" evidence="7">
        <location>
            <begin position="130"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003893-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="374" mass="40733" checksum="366A7B2C591F6EA2" modified="2023-06-28" version="1">MMPKRSRLGDVRGPISNGPMQSRPLVALLDGRDCSIEMPILKDVATVAFCDAQSTSEIHEKVLNEAVGALMWHTIILTKEDLEKFKALRIIVRIGSGVDNIDVKAAGELGIAVCNVPGYGVEEVADTTMCLILNLYRRTYWLANMVREGKKFTGPEQVRDAAQGCARIRGDTLGLVGLGRIGSAVALRAKVFGFNVSFYDPYLPDGIEKSLGLNRVYTLQELLFHSDCVSLHCTLNEHNHHLINEFTIKQMRPGAFLVNTARGGLVDDEALAVALKQGRIRAAALDVHENEPYNGALKDAPNLLCTPHAAFYSEAATTELREMAASEIRRAIIGNIPECLRNCVNKEYFLRSSTGGGAGGYSEEMAAKLLAGTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VJA2</accession>
    <name>A0A6E8VJA2_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the phosphatase 2A regulatory subunit B family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040226434.1">
        <property type="nucleotide sequence ID" value="XM_040370500.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040226435.1">
        <property type="nucleotide sequence ID" value="XM_040370501.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040226437.1">
        <property type="nucleotide sequence ID" value="XM_040370503.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_049463120.1">
        <property type="nucleotide sequence ID" value="XM_049607163.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_049463121.1">
        <property type="nucleotide sequence ID" value="XM_049607164.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VJA2"/>
    <dbReference type="EnsemblMetazoa" id="ACON004502-RB">
        <property type="protein sequence ID" value="ACON004502-PB"/>
        <property type="gene ID" value="ACON004502"/>
    </dbReference>
    <dbReference type="GeneID" id="120951677"/>
    <dbReference type="CTD" id="47877"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002552"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004502"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_036678"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001892">
        <property type="entry name" value="Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:002951">
        <property type="entry name" value="Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000009">
        <property type="entry name" value="PP2A_PR55"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018067">
        <property type="entry name" value="PP2A_PR55_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11871">
        <property type="entry name" value="PROTEIN PHOSPHATASE PP2A REGULATORY SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037309">
        <property type="entry name" value="PP2A_PR55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00600">
        <property type="entry name" value="PP2APR55"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01024">
        <property type="entry name" value="PR55_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01025">
        <property type="entry name" value="PR55_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU331113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004502-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="445" mass="51245" checksum="11FB534B8878F21F" modified="2020-08-12" version="1">MAGNGETSWCFSQIKGALDDDVTEADIISCVEFNHDGELLATGDKGGRVVIFQRDPSSKASTPRRGEYNVYSTFQSHEPEFDYLKSLEIEEKINKIRWLRRKNQSHFLLSTNDKTIKLWKVSERDKRVEGYNTREDNGSIRDPASINSLRVPSIKPMDLMVEASPRRIFANAHTYHINSISVNSDQETYLSADDLRINLWHLEITDQSFNIVDIKPANMEELTEVITAAEFHPTECNVFVYSSSKGTIRLCDMRSSALCDRHAKLFEEPEDVDTTNKSFFREIISSISDVKLSNSGRYMISRDYLSIKVWDLHMETKPIETYPVHEYLRTKLCSLYENDCIFDKFECCWNGNDSAIMTGSYNNFFRIFDRNTNKDVTLEASRDIIKPKTVLKPRKVCTGGKRKKDEISVDCLAFNKRILHTAWHPLENIIAVAATNNLFIFQDKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VJA5</accession>
    <name>A0A6E8VJA5_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Calcineurin is a calcium-binding and calmodulin-binding protein found in all cells from yeast to mammals, and a calcium-dependent, calmodulin-stimulated protein phosphatase.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the calcineurin regulatory subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VJA5"/>
    <dbReference type="EnsemblMetazoa" id="ACON004298-RB">
        <property type="protein sequence ID" value="ACON004298-PB"/>
        <property type="gene ID" value="ACON004298"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010643"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004298"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031110"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030431">
        <property type="term" value="P:sleep"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000047">
        <property type="entry name" value="Calcineurin subunit B type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45942">
        <property type="entry name" value="PROTEIN PHOSPATASE 3 REGULATORY SUBUNIT B ALPHA ISOFORM TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="EF-hand"/>
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        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
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    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="49"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="86"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="127"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00053611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004298-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="169" mass="19118" checksum="D5D18F14C191601B" modified="2020-08-12" version="1">GNETSLPMELCSNFDADEIRRLGKRFKKLDLDNSGALSIDEFMSLPELQQNPLVQRVIDIFDADGNGEVDFKEFIQGVSQFSVKGDKLSKLKFAFRIYDMDNDGYISNGELFQVLKMMVGNNLKDTQLQQIVDKTIVFADKDEDGKISFDEFCSVVGNTDIHKKMVVDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VJL0</accession>
    <name>A0A6E8VJL0_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="4">EGF-like domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_040240600.2">
        <property type="nucleotide sequence ID" value="XM_040384666.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VJL0"/>
    <dbReference type="EnsemblMetazoa" id="ACON004607-RA">
        <property type="protein sequence ID" value="ACON004607-PA"/>
        <property type="gene ID" value="ACON004607"/>
    </dbReference>
    <dbReference type="GeneID" id="120961060"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012539"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004607"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_036225"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039888261" evidence="3">
        <location>
            <begin position="23"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="115"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="180"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="291"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="182"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="196"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="244"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="296"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004607-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="335" mass="37281" checksum="CEDDBDD1307391D6" modified="2023-06-28" version="2">MDTTKIAYLMLAVCFSWLPALSGTTTTNPFHAALTDNLTQCGAVVCVAETEVCREDSFCECKPHFEDQGSFQCVPCPAEGQYCRGCCISDALTCYHGVCRRCSLDSNGNCIQESLFFLTAAQVALATAMVLGVISLSFLLYKTLRNRLRRNNQVLESEFRQSVVSRVSLSSIQQRVIRRLRDRPPKYETRHNYEYQQRQTERQISQQSSSVEPDRQSNPAPVGGPPPAYDGDTISLAENPPPYTQEQTDRTSSGIAVIDIPTTTTTDHALSMDLPTDRTSSNLQGITNHAFEPDKLTSSQPQIIQPSDNGETNGRPKEECYDIPLVNRNDKTVYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VLC6</accession>
    <name>A0A6E8VLC6_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Peptidoglycan recognition protein family domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VLC6"/>
    <dbReference type="EnsemblMetazoa" id="ACON005203-RF">
        <property type="protein sequence ID" value="ACON005203-PF"/>
        <property type="gene ID" value="ACON005203"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_014474"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005203"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033832"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008745">
        <property type="term" value="F:N-acetylmuramoyl-L-alanine amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009253">
        <property type="term" value="P:peptidoglycan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06583">
        <property type="entry name" value="PGRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.80.10:FF:000001">
        <property type="entry name" value="Peptidoglycan recognition protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.80.10">
        <property type="entry name" value="Peptidoglycan recognition protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036505">
        <property type="entry name" value="Amidase/PGRP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002502">
        <property type="entry name" value="Amidase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015510">
        <property type="entry name" value="PGRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006619">
        <property type="entry name" value="PGRP_domain_met/bac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11022">
        <property type="entry name" value="PEPTIDOGLYCAN RECOGNITION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11022:SF41">
        <property type="entry name" value="PEPTIDOGLYCAN-RECOGNITION PROTEIN LC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01510">
        <property type="entry name" value="Amidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00644">
        <property type="entry name" value="Ami_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00701">
        <property type="entry name" value="PGRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55846">
        <property type="entry name" value="N-acetylmuramoyl-L-alanine amidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0391">Immunity</keyword>
    <keyword evidence="2" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="218"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidoglycan recognition protein family" evidence="7">
        <location>
            <begin position="280"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetylmuramoyl-L-alanine amidase" evidence="6">
        <location>
            <begin position="292"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="170"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="8"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="50"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="171"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005203-PF"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="447" mass="48562" checksum="566E346349109109" modified="2020-08-12" version="1">MALARNLQLENDTSVNTGYHTKAQSMSASGGDTIAGLNNSGTGTGKSDSAKGSSLSNISASSIKRTDNGGDDDDSSVFDSSSSDTECDDDSIKRAIDRIPGTLAPGEARVLPNANVKVAVEAIAPGVRPSPAASTIGAIAVHNSSDITFGNKTYIKGQVVIKNIYQDRKNGTTNQGYQVQENETDDTNSTKKVPSATKPPEPRTWQSSLKTIIKDKPLLSFIVMVSLMIVLCAIVAVISILTASGKARFRLPVGDGDDNRQNIPQDKDIEDDLFPDPRPLRLVTRTEWLAQPPREELTDLKLPVNNVIIAHTATEGCTTQQACKALVQSIQRYHVNHTNYGDIGYNFLIGGDEFVYEGRGWLKVGAHTKNYNTISYGIAFIGNYETKDRPTEQQMEQLVLLLRNGTDGGWLAKEYKLCGASQLKGTISPGKYLMEQLRLLPHFSERL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VLD8</accession>
    <name>A0A6E8VLD8_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Aldehyde dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4 7">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VLD8"/>
    <dbReference type="EnsemblMetazoa" id="ACON005124-RE">
        <property type="protein sequence ID" value="ACON005124-PE"/>
        <property type="gene ID" value="ACON005124"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006213"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005124"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031069"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004029">
        <property type="term" value="F:aldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006081">
        <property type="term" value="P:aldehyde metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07132">
        <property type="entry name" value="ALDH_F3AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000003">
        <property type="entry name" value="Aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000004">
        <property type="entry name" value="Aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012394">
        <property type="entry name" value="Aldehyde_DH_NAD(P)"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43570">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43570:SF16">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE TYPE III, ISOFORM Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036492">
        <property type="entry name" value="ALDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="2 4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="469"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="9">
        <location>
            <begin position="7"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="active site" evidence="5 6">
        <location>
            <position position="209"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR036492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR036492-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005124-PE"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="494" mass="54940" checksum="5DEEB2CBD11256CC" modified="2020-08-12" version="1">MSFSDIVQQLRTTFNSGKTRDVDFRLNQLKALLRCYEENTAEMANVLAADLRKHKQEAHLLEIEFILNDLRNTLYNLREWVKPEKPEKSLVNVMDGVYIYKDPYGVVLVIGAWNYPLQLTLVPVAAAIAAGNCVVIKPSEVAPASAKFIAETLPKYLSQDCYRVVTGGPKETSELLKEKFDYVFYTGSGRVGKIIHQACSENLTPCTLELGGKSPCYLDGTANIAIAARRILWGKFINAGQTCIAPDYILCSKQVQKQFLEEARKVLNEWYGANPKDSPDLCRIINQNHFQRLTSLLKGANVAIGGETDLQDRYISPTILIDVKSTDPVMQDEIFGPILPILNVDNAYDAIKFINSRDKPLCLYLFTENIEDRDAFIANTSSGGVCVNDALTHFAVEALPFGGVGPSGMGSYHGKYSFDTFVHKKSCLTKDFNPIGEKLAASRYPPYSESKLSFLSTLLKKRQGINLHFLPYLLMFGIGVASTLVVSTILKDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VLE5</accession>
    <name>A0A6E8VLE5_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Short-chain dehydrogenase/reductase 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Retinal short-chain dehydrogenase/reductase 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the reduction of all-trans-retinal to all-trans-retinol in the presence of NADPH.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VLE5"/>
    <dbReference type="EnsemblMetazoa" id="ACON005166-RB">
        <property type="protein sequence ID" value="ACON005166-PB"/>
        <property type="gene ID" value="ACON005166"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010823"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005166"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031200"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052650">
        <property type="term" value="F:all-trans-retinol dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000131">
        <property type="entry name" value="Short-chain dehydrogenase/reductase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24322:SF748">
        <property type="entry name" value="FI23927P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24322">
        <property type="entry name" value="PKSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00059620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00068717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00082544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005166-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="316" mass="34895" checksum="5E6314D9A86BBB59" modified="2020-08-12" version="1">MATEVPYFTNAENGYVPAKLAHRSAASSQPLRLLRFVVNHVIPDTLKFLLQLIPLALSGLVGLVLPSKKKSIHGHTALVTGGANGLGRALCLRLAREGCQVAVVDIDLAGAQRTVEDVRALGVKAEAFLADIANYEAVERLRLEVESKLGPVDVLVNNAGLLAVLSLSEGKPADLERIVNVNLLSHFWTIRAFMPGMITRHRGHIVGIASIAAYFPVGRFIPYTVTKYAVRALMESLNSELRMDGLQNTIQTTCVYPALIATRQQFMDMLDKLNFLKRFYVFTPEQVAEQIVWGVLINKREVFVPNIMALFSKQFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VLG2</accession>
    <name>A0A6E8VLG2_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">HTH psq-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="gene ID" value="ACON005244"/>
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        <property type="entry name" value="HTH_Psq"/>
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        <property type="entry name" value="BTB DOMAIN TRANSCRIPTION FACTOR"/>
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        <property type="entry name" value="PIPSQUEAK, ISOFORM O"/>
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        <property type="entry name" value="HTH_psq"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="311"/>
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            <end position="417"/>
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        <location>
            <begin position="420"/>
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    <feature type="DNA-binding region" description="H-T-H motif" evidence="4">
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            <begin position="339"/>
            <end position="359"/>
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            <begin position="393"/>
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        <location>
            <begin position="448"/>
            <end position="468"/>
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            <begin position="43"/>
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            <begin position="128"/>
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            <begin position="524"/>
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            <begin position="128"/>
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            <begin position="545"/>
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    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50960"/>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
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    </evidence>
    <sequence length="756" mass="82691" checksum="D05751942D851AF4" modified="2020-08-12" version="1">MASLGMGVGINGGSVMGVPMGYLDFAPEPPAPTATPVTEHVDINCAPSSHDTRDLSNSVPNDGDLRVKFETLRSLDPPNAVQLDNHTVSHQQQLHHQQQQAQQQAQQQAQQQQLLQQQQHLLQQQQQQQQQQQRQTPQPAGAQQQQNDHQRHSPPHMQQQQQQPQQHVMLMDQQQQQHHPTTHSPASSVSSSSSSQHPENLVTGSGGAAGSVTPGPPTNNSNGNGAGGDELKPRLNLKQENISDEQQHQTMEVEQDQSSMHGMVVTPEISGIMNQSQMSDMYQSDASEDSKVGILDGSSMQYTNLTSPNEAKTPTGPKTWTAEDMESALEALRSHNMSLTKASATYGIPSTTLWQRAHRLGIDTPKKEGPSKTWNEDSLNSALEALRTGTISANKASKAFGIPSSTLYKIARREGIRLAAPFNAAPTTWSAEDLDRALEAIRAGQTSVQKASTEFGIPTGTLYGRCKREGIELSRSNPTPWSEDAMMEALESVKQGHMSINQAAIHYNLPYSSLYGRFKRGKYDTPSSGGGGGGSSSNAAAAAATGGGGAGSGGGSGAGSAASAGTGGNNVGSSPQMEFKIEPQHNDHSPENTQHYNPALSASSTPTSNTPTQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQLTHHLVQQPPSVLPVSIHIVDAAAHHHLAHPHQQLHHQQQQLAQPQHVVVAQHQQQQQHHHLHHQPQLIQHHIIPQQHHIIYQQPLQPIQQQPQYHPMYHIIKQENDRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VLS5</accession>
    <name>A0A6E8VLS5_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="13">BTB domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Putative transcription factor required for axon growth and guidance in the central and peripheral nervous systems. Repels CNS axons away from the midline by promoting the expression of the midline repellent sli and its receptor robo.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VLS5"/>
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        <property type="protein sequence ID" value="ACON005245-PC"/>
        <property type="gene ID" value="ACON005245"/>
    </dbReference>
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    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012771"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005245"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_029613"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:axon midline choice point recognition"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:dendrite morphogenesis"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:gonad development"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0035167">
        <property type="term" value="P:larval lymph gland hemopoiesis"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:nurse cell apoptotic process"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:R3/R4 cell fate commitment"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Lola, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
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    <dbReference type="InterPro" id="IPR051095">
        <property type="entry name" value="Dros_DevTransReg"/>
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        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
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        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
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        <property type="entry name" value="BTB DOMAIN TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110:SF111">
        <property type="entry name" value="LONGITUDINALS LACKING PROTEIN, ISOFORMS F_I_K_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3" id="KW-0221">Differentiation</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="11">
        <location>
            <begin position="32"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="431"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="461"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="115"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="301"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="485"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="548"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="161"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="175"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="212"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="322"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="496"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="551"/>
            <end position="561"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00037382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005245-PC"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="616" mass="65283" checksum="AD57EF5BD729DAAA" modified="2020-08-12" version="1">MDDDQQFCLRWNNHQSTLISVFDTLLENGTLVDCTLAAEGKLLKAHKVVLSACSPYFATILSQQYDKHPIFILKDVKFQELRAMMDYMYRGEVNISQDQLAALLKAAESLQIKGLSDNRSSSSSGTAAAGGGVHQKQSDAPAAKTLQPPVPASKASGLTIENKRSLKSELLESDVSGSREGSTSPTSRKRKKVRRRSVDTNNLLIDNHDQHSNSSSHSMHTAGGANVAAAVAAAGAPAANVASSLSSASSTAAAGSNTSANVLSVAAVAAAAVLKKTETVQQAAAETLKLQKQQAAAAAAAAAAQSSDDEERCGPGSVATGTEHDDGNDLSDNEHDGDGGSSVKSGSRHHHLRDGSRRGKTSTSSMGEMMIEPKSEYEDVQDENVEDLTMDDEDLMDELDQAGPSHGGDVSNYKMTSLLYSNFLDITMQRFRCSVCDKSYLRKRHLQRHMRDECIGIPPRFHCDHCDSKFRRKYHLVRHMLSKHGIQMEPGPMKPGSGGSGANGGGSGSGTEGDGSEMKYVKLAAEAGNGELATIAAAAAAAAAAAANTKGGKGGGKGSRAGTGAEDSADEKFSMVDTLMVKQEEIEPVSSQTMMYQNFKNLFFDYALKNVPAPMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VNH5</accession>
    <name>A0A6E8VNH5_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Fatty acyl-CoA reductase</fullName>
            <ecNumber evidence="4">1.2.1.84</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the reduction of fatty acyl-CoA to fatty alcohols.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a long-chain fatty acyl-CoA + 2 NADPH + 2 H(+) = a long-chain primary fatty alcohol + 2 NADP(+) + CoA</text>
            <dbReference type="Rhea" id="RHEA:52716"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:77396"/>
            <dbReference type="ChEBI" id="CHEBI:83139"/>
            <dbReference type="EC" id="1.2.1.84"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the fatty acyl-CoA reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.84" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8VNH5"/>
    <dbReference type="EnsemblMetazoa" id="ACON005984-RA">
        <property type="protein sequence ID" value="ACON005984-PA"/>
        <property type="gene ID" value="ACON005984"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005345"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005984"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031243"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102965">
        <property type="term" value="F:alcohol-forming long-chain fatty acyl-CoA reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080019">
        <property type="term" value="F:alcohol-forming very long-chain fatty acyl-CoA reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035336">
        <property type="term" value="P:long-chain fatty-acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05236">
        <property type="entry name" value="FAR-N_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09071">
        <property type="entry name" value="FAR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000639">
        <property type="entry name" value="Fatty acyl-CoA reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026055">
        <property type="entry name" value="FAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033640">
        <property type="entry name" value="FAR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013120">
        <property type="entry name" value="FAR_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11011:SF60">
        <property type="entry name" value="FATTY ACYL-COA REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11011">
        <property type="entry name" value="MALE STERILITY PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07993">
        <property type="entry name" value="NAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03015">
        <property type="entry name" value="Sterile"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="3 4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="395"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="509"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="domain" description="Thioester reductase (TE)" evidence="6">
        <location>
            <begin position="39"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acyl-CoA reductase C-terminal" evidence="5">
        <location>
            <begin position="400"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005984-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="538" mass="62092" checksum="CD4689C39939BD6E" modified="2020-08-12" version="1">MDLRASAESGHNNELTMTEERKITSTPVMEFYRDKCVLITGGTGFIGRLLIEKLLRINVRQIILLSRPKKGKTTQQRCDDLFSSIVFMNLKKDCPTFIERVKLVDADLQHPSLGLSDESIEYIVNNAQIVLHAASDVRFDQALKKAIEVNVRGTRDLLRIAEKIVNLELFVYISTAYSNCPQGLIKEQFYTPPSEPEKMIQLVEAMDERFEEHMNKTVNDFIHPWPNTYVYTKALTEDVVRQYGELLPIAVVRPSIVIATNEEPIGGWTDNIYGLNGVIAGVALGIIRIMHVDDNNKADIIPADIVVNTVLAAGWQTYVERKGDRPLPEAKTNGELKGVAKPRTKIYNCVTGNDNPISYQKIYKYSIEVGKHCPPKKSLWIVCHNTTTNKYLYEFYKVIYHLLPALLIDTYLRVIRRTPRVMDLYRKVHKFATVIEYFANGRWTFENDNLKSLREKLSPDDQIMFQCNIQKIEWADYFWTYIHGLRKHIANEPLENLDEAIKRHKQMRIVHYFILAAYYSIWALLIFYLCKAVGMLVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VNI5</accession>
    <name>A0A6E8VNI5_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Asp_Arg_Hydrox domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VNI5"/>
    <dbReference type="EnsemblMetazoa" id="ACON005832-RB">
        <property type="protein sequence ID" value="ACON005832-PB"/>
        <property type="gene ID" value="ACON005832"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_003003"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005832"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_036948"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062101">
        <property type="term" value="F:peptidyl-aspartic acid 3-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039038">
        <property type="entry name" value="ASPH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12366:SF29">
        <property type="entry name" value="ASPARTYL BETA-HYDROXYLASE, ISOFORM L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12366">
        <property type="entry name" value="ASPARTYL/ASPARAGINYL BETA-HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="62"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="130"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="476"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="533"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="146"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="207"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="219"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="248"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="271"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="304"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="335"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="352"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="391"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="479"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="500"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="533"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="561"/>
            <end position="578"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005832-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="587" mass="65065" checksum="DE109B8B328697A5" modified="2020-08-12" version="1">MSGDTQAKKRKDKKRKKDDEDTVKETTVHAPKQPSAGAAQAAKEPGDVQMHIQSDHGTGGHWCAKVVFFILLAGLGALIGLIIMENQGVSNEDTPLSESRYSEFFNGWVDENRQDDHHHDEVLAAINSLDDHDDEGDAHHAVEDHDGGDDDGHDQDGDDDDHDDGAPYAEEEDHDDEEDGTEVQDDDEDEGPASKLDDDEVEEIYTQQEAAEQQQAAAADDDDDGDDDGQDDDDEDDDAGANDHTNAVDDDDNDDDNDDDKNEETVVGQDNDNDQDDQDDDDQNDKNEAEDETDNVVTNSKTDLEDDNDDNDDEQDENDDDNDKDNNDNENAAAEQDDDDENEEDNNKNVDSEEEPAGNADDDDDNQNEAANEDDDDDQDDTPFDEVKRSNALNDNANRNVPTINEDTVQTSVPEPERNQVPVTTTVPSTEGAVTGDTLKDQMDELVRNYNKLAESLDVPKVEEVVDVPDTIAAQVNEDANDDGDEEEILEVTRGSQPANDDDDDDVDDDDAAFSELVDNDGGEEEYLEKLRAEQQRRAAAEAERRAAAEPEEETSQIPSRAEEADRSYQEELRRAEQQLDEEFGFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VNK4</accession>
    <name>A0A6E8VNK4_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Tyrosine 3-monooxygenase</fullName>
            <ecNumber evidence="6">1.14.16.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Tyrosine 3-hydroxylase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">Ngousso</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 18">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Axon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Perinuclear region</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.</text>
    </comment>
    <dbReference type="EC" id="1.14.16.2" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8VNK4"/>
    <dbReference type="EnsemblMetazoa" id="ACON006023-RA">
        <property type="protein sequence ID" value="ACON006023-PA"/>
        <property type="gene ID" value="ACON006023"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005497"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON006023"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030607"/>
    <dbReference type="UniPathway" id="UPA00747">
        <property type="reaction ID" value="UER00733"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043204">
        <property type="term" value="C:perikaryon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004511">
        <property type="term" value="F:tyrosine 3-monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048066">
        <property type="term" value="P:developmental pigmentation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006585">
        <property type="term" value="P:dopamine biosynthetic process from tyrosine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03345">
        <property type="entry name" value="eu_TyrOH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.800.10:FF:000004">
        <property type="entry name" value="Tyrosine 3-monooxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.800.10">
        <property type="entry name" value="Aromatic amino acid hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001273">
        <property type="entry name" value="ArAA_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018301">
        <property type="entry name" value="ArAA_hydroxylase_Fe/CU_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036951">
        <property type="entry name" value="ArAA_hydroxylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036329">
        <property type="entry name" value="Aro-AA_hydroxylase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019774">
        <property type="entry name" value="Aromatic-AA_hydroxylase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041903">
        <property type="entry name" value="Eu_TyrOH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005962">
        <property type="entry name" value="Tyr_3_mOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019773">
        <property type="entry name" value="Tyrosine_3-monooxygenase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01269">
        <property type="entry name" value="Tyr_3_monoox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11473">
        <property type="entry name" value="AROMATIC AMINO ACID HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11473:SF15">
        <property type="entry name" value="TYROSINE 3-MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00351">
        <property type="entry name" value="Biopterin_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000336">
        <property type="entry name" value="TH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00372">
        <property type="entry name" value="FYWHYDRXLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56534">
        <property type="entry name" value="Aromatic aminoacid monoxygenases, catalytic and oligomerization domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00367">
        <property type="entry name" value="BH4_AAA_HYDROXYL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51410">
        <property type="entry name" value="BH4_AAA_HYDROXYL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0127">Catecholamine biosynthesis</keyword>
    <keyword evidence="15" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13 17" id="KW-0408">Iron</keyword>
    <keyword evidence="10 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="11" id="KW-0530">Neurotransmitter biosynthesis</keyword>
    <keyword evidence="12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Biopterin-dependent aromatic amino acid hydroxylase family profile" evidence="20">
        <location>
            <begin position="224"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="106"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="107"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="118"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="403"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="408"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="448"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00017181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00032379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR000336-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR601273-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON006023-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="574" mass="65467" checksum="B12B7BA0EC414D1A" modified="2020-08-12" version="1">MHTAQAPVMSIQIFQIFLEECDENGYPSRRRSLVDDARFETIVVKQTKQTVLDEARAKANDSSLECTILQAQEQHQQEDKIPQEVQQTVEDQNDDEEEIRMVAVDELPQKPQEHVPSADDEDKETDAGLTEEEVVLQNAASESPEAEKEVVRAAVVVRLRDGMGSLGRILKAVEAYHGTVVHLESRQSRSEGVQFDVLVKVDMARANLLQLIRSLRQTQSFGSVSLLSENNVNVKAPWFPKHASELDNCNHLMTKYEPDLDMNHPGFADQVYRARRKEIAEIAFAYRYGDPIPHIDYTETENKTWAAVFGRVKELMVQHACSEYIAVFRKLEDEKIFVKERLPQLQEMSDFLRKNTGFTLRPAAGLLTARDFLASLAFRIFQSTQYVRHINSPYHTPEPDCIHELLGHMPLLADPSFAQFSQEIGLASLGASDEEIEKLSTVYWFTVEFGLCKEKDEVKAYGAGLLSAYGELLHAISDKPEHRPFEPASTAVQPYQDQEYQPIYYVAESFEDAKEKFRRWVSTMSRPFEVRFNPHTERVEVLDSVDKLETLVSQLNTEVLHLTNAIAKLKQPFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VNW5</accession>
    <name>A0A6E8VNW5_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Gamma-aminobutyric acid receptor subunit beta</fullName>
        </recommendedName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="24">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="18">Postsynaptic cell membrane</location>
            <topology evidence="18">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040228039.1">
        <property type="nucleotide sequence ID" value="XM_040372105.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VNW5"/>
    <dbReference type="EnsemblMetazoa" id="ACON006028-RA">
        <property type="protein sequence ID" value="ACON006028-PA"/>
        <property type="gene ID" value="ACON006028"/>
    </dbReference>
    <dbReference type="GeneID" id="120952660"/>
    <dbReference type="CTD" id="39054"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011712"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON006028"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033065"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005230">
        <property type="term" value="F:extracellular ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004890">
        <property type="term" value="F:GABA-A receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099095">
        <property type="term" value="F:ligand-gated monoatomic anion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19008">
        <property type="entry name" value="LGIC_ECD_GABAR_RDL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19049">
        <property type="entry name" value="LGIC_TM_anion"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000021">
        <property type="entry name" value="Gamma-aminobutyric acid receptor isoform 3b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000033">
        <property type="entry name" value="Gamma-aminobutyric acid receptor subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006028">
        <property type="entry name" value="GABAA/Glycine_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002289">
        <property type="entry name" value="GABAAb_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00860">
        <property type="entry name" value="LIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01160">
        <property type="entry name" value="GABAARBETA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00253">
        <property type="entry name" value="GABAARECEPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-0868">Chloride</keyword>
    <keyword evidence="12" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="17 20" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 20" id="KW-0406">Ion transport</keyword>
    <keyword evidence="16" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="9 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 20" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0770">Synapse</keyword>
    <keyword evidence="4 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 20" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026379843" description="Gamma-aminobutyric acid receptor subunit beta" evidence="20">
        <location>
            <begin position="35"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="262"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="297"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="328"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="520"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="22">
        <location>
            <begin position="56"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="23">
        <location>
            <begin position="269"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="367"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="444"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="21">
        <location>
            <begin position="393"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="406"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="21">
        <location>
            <begin position="461"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00071250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON006028-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="555" mass="61310" checksum="0B7EBE84B5BEB97F" modified="2020-08-12" version="1">MSLTIEVPHAKSPSLGVLILTLNLALFLPQTINRTPPYVLAGTGGGSMLGDVNISAILDSFSVGYDKRVRPNYGGPPVEVGVTMYVLSISSLSEVKMDFTLDFYFRQFWTDPRLAYRKRPGVETLSVGSEFIKNIWVPDTFFVNEKQSYFHIATTSNEFIRIHHSGSITRSIRLTITASCPMNLQYFPMDRQLCHIEIESFGYTMRDIRYFWKDGLSSVGMSSEVELPQFRVLGHRQRATEINLTTGNYSRLACEIQFVRSMGYYLIQIYIPSGLIVIISWVSFWLNRNATPARVSLGVTTVLTMTTLMSSTNAALPKISYVKSIDVYLGTCFVMVFASLLEYASVGYMAKRIQMRKQRFMAIQKIAEQKKQQQADANHPPPPPPVASDHSHGHGHGHSHTHQHTPKQQMGSRSGTMQNVPPNMGSRGCSIVGPLFQEVRFKVHDPKAHSKGGTLENTINGGRGGGQPGGGGPPPGGPPGGGGGGPDEESAAPQHLIHPGKDINKLLGITPSDIDKYSRIVFPVCFVCFNLMYWIIYLHVSDVVADDLVLLGEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VPM5</accession>
    <name>A0A6E8VPM5_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GTP cyclohydrolase 1</fullName>
            <ecNumber evidence="5">3.5.4.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">GTP cyclohydrolase I</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>GTP + H2O = 7,8-dihydroneopterin 3'-triphosphate + formate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17473"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58462"/>
            <dbReference type="EC" id="3.5.4.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; 7,8-dihydroneopterin triphosphate biosynthesis; 7,8-dihydroneopterin triphosphate from GTP: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Toroid-shaped homodecamer, composed of two pentamers of five dimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the GTP cyclohydrolase I family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.16" evidence="5"/>
    <dbReference type="RefSeq" id="XP_040224386.2">
        <property type="nucleotide sequence ID" value="XM_040368452.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VPM5"/>
    <dbReference type="EnsemblMetazoa" id="ACON006441-RA">
        <property type="protein sequence ID" value="ACON006441-PA"/>
        <property type="gene ID" value="ACON006441"/>
    </dbReference>
    <dbReference type="GeneID" id="120950439"/>
    <dbReference type="CTD" id="37415"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000238"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON006441"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037131"/>
    <dbReference type="UniPathway" id="UPA00848">
        <property type="reaction ID" value="UER00151"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003934">
        <property type="term" value="F:GTP cyclohydrolase I activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046148">
        <property type="term" value="P:pigment biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006729">
        <property type="term" value="P:tetrahydrobiopterin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00642">
        <property type="entry name" value="GTP_cyclohydro1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.286.10:FF:000003">
        <property type="entry name" value="GTP cyclohydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1130.10:FF:000012">
        <property type="entry name" value="GTP cyclohydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.286.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00223">
        <property type="entry name" value="FolE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043133">
        <property type="entry name" value="GTP-CH-I_C/QueF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043134">
        <property type="entry name" value="GTP-CH-I_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001474">
        <property type="entry name" value="GTP_CycHdrlase_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018234">
        <property type="entry name" value="GTP_CycHdrlase_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020602">
        <property type="entry name" value="GTP_CycHdrlase_I_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00063">
        <property type="entry name" value="folE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006825">
        <property type="entry name" value="PRK09347.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006826">
        <property type="entry name" value="PRK09347.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11109:SF7">
        <property type="entry name" value="GTP CYCLOHYDROLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11109">
        <property type="entry name" value="GTP CYCLOHYDROLASE I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01227">
        <property type="entry name" value="GTP_cyclohydroI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55620">
        <property type="entry name" value="Tetrahydrobiopterin biosynthesis enzymes-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00859">
        <property type="entry name" value="GTP_CYCLOHYDROL_1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00860">
        <property type="entry name" value="GTP_CYCLOHYDROL_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0783">Tetrahydrobiopterin biosynthesis</keyword>
    <feature type="domain" description="GTP cyclohydrolase I" evidence="13">
        <location>
            <begin position="106"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="7"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="27"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00017272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON006441-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="285" mass="31487" checksum="21E4D5B9F3BB92D1" modified="2020-08-12" version="1">MKEVQVNGHQHVSNGNGTVSELEKHTNGTSNGYAGHNGLSNGTNGTTSNGCASSKKQSVKCTCSKSELENGSDTNNNQGHEKCTFHHDLELDHKPSTREDLLPLMSKSYRMLLSSLGEDPERQGLLKTPERAAKAMLFFTKGYDQSLEEALNGAVFDEDHDEMVVVKDIEMFSMCEHHLVPFYGKVSIGYLPCKKILGLSKLARIVEIFSRRLQVQERLTKQIAVAVTQAVQPAGVAVIIEGVHMCMVMRGVQKINSKTVTSTMLGVFRDDPKTREEFLTLCNNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VPZ2</accession>
    <name>A0A6E8VPZ2_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Cyclic nucleotide-binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cAMP-dependent kinase regulatory chain family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040218589.1">
        <property type="nucleotide sequence ID" value="XM_040362655.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VPZ2"/>
    <dbReference type="EnsemblMetazoa" id="ACON006448-RD">
        <property type="protein sequence ID" value="ACON006448-PD"/>
        <property type="gene ID" value="ACON006448"/>
    </dbReference>
    <dbReference type="GeneID" id="120947380"/>
    <dbReference type="CTD" id="40305"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_008284"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON006448"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042399"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030552">
        <property type="term" value="F:cAMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004862">
        <property type="term" value="F:cAMP-dependent protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034236">
        <property type="term" value="F:protein kinase A catalytic subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007611">
        <property type="term" value="P:learning or memory"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000013">
        <property type="entry name" value="cAMP-dependent protein kinase type I regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000006">
        <property type="entry name" value="cAMP-dependent protein kinase type I-alpha regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050503">
        <property type="entry name" value="cAMP-dep_PK_reg_su-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012198">
        <property type="entry name" value="cAMP_dep_PK_reg_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE REGULATORY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635:SF152">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE TYPE I REGULATORY SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000548">
        <property type="entry name" value="PK_regulatory"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00103">
        <property type="entry name" value="CAMPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00888">
        <property type="entry name" value="CNMP_BINDING_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00889">
        <property type="entry name" value="CNMP_BINDING_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0114">cAMP</keyword>
    <keyword evidence="3 7" id="KW-0116">cAMP-binding</keyword>
    <keyword evidence="5 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="9">
        <location>
            <begin position="77"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="9">
        <location>
            <begin position="195"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>3',5'-cyclic AMP</name>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>3',5'-cyclic AMP</name>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="266"/>
        </location>
        <ligand>
            <name>3',5'-cyclic AMP</name>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>3',5'-cyclic AMP</name>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000548-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON006448-PD"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="321" mass="35669" checksum="FA93D8657E3D8350" modified="2020-08-12" version="1">MSEEQAKLDASKQAVTPDDLDDLSPMPQTAVPPVRRRGGISAEPVTEEDATSYVKKVVPKDYKTMAALSKAIAKNVLFSHLDENERSDIFDAMFPCNFLPGEPIIQQGDEGDNFYVIDIGEVEVFVNNEQVTTISEGGSFGELALIYGTPRAATVRAKTDVKLWGIDRDSYRRILMGSTIRKRKMYEEFLSRVSILESLDKWERLTVADALEPVSFEDGETIVRQGEPGNDFYIIVEGCATVMQKRGDSEEAAEVGRLGPSDYFGEIALLLDRPRAATVIARGPLKCVKLDRARFERVLGLCADILKRNITQYNSFVSLSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VPZ6</accession>
    <name>A0A6E8VPZ6_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Thioredoxin domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VPZ6"/>
    <dbReference type="EnsemblMetazoa" id="ACON006452-RB">
        <property type="protein sequence ID" value="ACON006452-PB"/>
        <property type="gene ID" value="ACON006452"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_013190"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON006452"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037069"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd02961">
        <property type="entry name" value="PDI_a_family"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TRX_superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19991">
        <property type="entry name" value="L 2 01289"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19991:SF3">
        <property type="entry name" value="LETHAL (2) 01289, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13848">
        <property type="entry name" value="Thioredoxin_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026151880" evidence="2">
        <location>
            <begin position="30"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="3">
        <location>
            <begin position="35"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="706"/>
            <end position="794"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
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            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON006452-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="821" mass="93864" checksum="7A4E40B7A5AB73B8" modified="2020-08-12" version="1">MGLPRIRSLVAPLCFLLLASSWLVSDVDCAKKAAASAPAAAPEATIEEVSAKQLERLLEDKDYVAVYWYARSCTTCDTVLAELEHIDDDTDSFGVDFVKINDKRLAKQYGITKFPALTYFREKEPIIYEGDLMDEAGVLDFLTSLEAMDLPDRIEEVNAKILSKIIEDSDYHVAVLFYDKDSKTSQKVLAELENIDDECDQNDIAFVKIDDDNEAKEWGIEELPTMILFERGIPHIYEGDLLKEEELLGWLVHQKRHSEIPEITDEMKDKLMQMYDHVAIIFYDKDDKQDIRVLNELENIDDELEKEEIVIARLDNAEEAREYGLDHLPALVYFENEIPAIYEGDLMNEEEVLEWLKLQKYSATIEEVTDEILQDLIDDHEYVCVYFSGACEEGEKCDKILDDLENIDDELDEAGIIFVTTEDMVTAKKYNIKNLPSLVFFRNKDPLIYSGDLNDEDEVLAWLTDEETLEIPGKIEEVNIKMLEKILSENEHIVVFFYHDDDKKAQKIIAELENIDDECEEKDIDFVKTSDEDIDKEYDLETLPALVFYRNKFRTIYTGDMMKEEEILEWVLEQYNTKPEIIESVDRKTLQVLVNEVEHLAVLFYDDDCETCPKILEKLETIDDDTDKHHIQFVKANDEKLAHEIGIFSFPALVYYETGVPIMYDGNLKNEKRILQWLIDHKNNKLPKPKLRKRYTDGVEDLDREDRIDKKRSGGKKLKPIPRLDAVEQPKAPGAGAGTSGKDSGKSKTTKSAPAPAPPASKSGKSDTGKAAPSPSKGSNVAKKTTRDVDDDEGSLFLSRSSLSFSVFRSLQELLVEESVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VQ08</accession>
    <name>A0A6E8VQ08_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="EnsemblMetazoa" id="ACON006590-RA">
        <property type="protein sequence ID" value="ACON006590-PA"/>
        <property type="gene ID" value="ACON006590"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005456"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009145"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_013043"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON006590"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_036154"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038827"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039327"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00136">
        <property type="entry name" value="PDZ_canonical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01230">
        <property type="entry name" value="PH1_Tiam1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01255">
        <property type="entry name" value="PH2_Tiam1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000337">
        <property type="entry name" value="Protein still life, isoform SIF type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000373">
        <property type="entry name" value="Protein still life, isoform SIF type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000065">
        <property type="entry name" value="T cell lymphoma invasion and metastasis 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.680">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Dbl homology (DH) domain"/>
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        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
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        <property type="entry name" value="DBL_dom_sf"/>
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        <property type="entry name" value="DH_dom"/>
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        <property type="entry name" value="GDS_CDC24_CS"/>
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        <property type="entry name" value="PDZ"/>
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        <property type="entry name" value="PDZ_sf"/>
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        <property type="entry name" value="PH_domain"/>
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        <property type="entry name" value="PH_Tiam1/2"/>
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        <property type="entry name" value="RBD_dom"/>
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        <property type="entry name" value="Tiam1/Tiam2/Sif"/>
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        <property type="entry name" value="TIAM1_CC-Ex"/>
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        <property type="entry name" value="WH1/EVH1_dom"/>
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        <property type="entry name" value="TIAM (MAMMALIAN TUMOR INVASION AND METASTASIS FACTOR) HOMOLOG"/>
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        <property type="entry name" value="PH_Tiam1"/>
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        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Tiam_CC_Ex"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
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        <location>
            <begin position="80"/>
            <end position="198"/>
        </location>
    </feature>
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            <begin position="882"/>
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        <location>
            <begin position="1161"/>
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            <begin position="1244"/>
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            <begin position="258"/>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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        </source>
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        </source>
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        </source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="2736" mass="303892" checksum="B2E8AB8AC43D2D8B" modified="2020-08-12" version="1">MGAKGSVEAAKMNADGWGHFERGSRHYSHRHNQRSQSLNRGQALSRADVSGICAMGNKLSCSCAPLMRKAYRYEDSPWQASRRRDGHLLRLWAEVFHVSASGAGTVKWQQVSEDLVPVNITCIQDSPECVFHITAYNSQVDKILDVRLVQPGTRIGQASECFIYWKDPMTNDTWGLNFTSPIDAKQFRECCSPSFKFSRKASSSYSLKLDPPGKGKVKTKRKPLSTPASPSRAREPQCTCMTAEQYARIRAQDPRYRGSSTLPRATNRATETETLRSEKIATATSSTSLYDNVGANNTQGNTINSQEAGHRQTKSQETSTMTNVGTVTGTAVTQNHVGSQIGQDESGSAMGNQRPEETSKSEGTQAGGTLQQPERSTKTTSTGTGTRTKDFNEEMPRDQHDMHHNIINNNTARRNKSKSTEDMNVDSSTMKRMLKPMPSAESPVTSPEMGRRRYNYYNSATNTPHTHQHTIHNAHMLNNNGTISRNASQSSRFSGSRSSHEIGRGHQHGGRGLYLELERDRSCVEGSPPSDNVMFDNQCYATTPSSSNGNSDQDQPPYGRQSGRHSHHHQVGLAGPIAPQWWRGNPPNVTPTPGSPTSRLLLEYEMHLRNTLAKGMDAESYSLHTFEALLSQSMENLELAESLPGSNQRSSAKSSTLPLPHRLGGNERPVTVRERDRDRDGYYSDRNELIRERERDRDRDRGYLSDHNSNYSTTTRCASCIGESARAQWFRHSDGWRSGSSTFGSGSGPGLVSSQSNAGGGHKRSPWDSLPSLRQDSSLNDSGYKSNRADSFEQRGVFDRQDSLRSDYMSDREPRYGIVQQASIDSTDSRLCYLTSSEISDDDRMSLTTAVSDEDDGESVMASPYKAKATGTAAASFNCTGAVRKAGFLSVKKWLLRKKHQIELARKRGWKGYWVCLKGTTLLFYPCDSREGRSVEAAPKHLIIVDGAIMQPIPEHPKRDYIFCLSTAFGDAYLFQAPCQVELENWVNSIHSACAAAFARHRGKTGTLHLLQEEIFRLEKAIESDHKLKHMADLQQSVVTDADTRHQIQQQILQWEENLERLHCEQFRLRCYMASLQNGELPNPKSLLTHVSRPTKNTLNKLGVFTVSSFHAFICARSPSLLNNLLAGRGATKRRPPILSRSNSGSSRRSMQINSRDEPEKSYKVALPENTFATVYLREGMSVEEFLASACSRKNLNPMEHFVRVKKRRDMEDHNYFVPHRNDLIETYLHTHEVVEVCAKILYQVELQRTTLEQMWGFSVEAELIENADRQDELCCYVSRVEDKSVAMQNGVIKGDEIIVINGAIVSDLDMMYLESVLQEEQALCMMMRSSRTEPPDLVGIMRSTDDIIESLVCPPPPSDPPVISEEMISGLIVPAPGWRKEVYSPEVESSPAPSSNDPHLLSMDRSKPSSRTSSFEIENLLKSAEQVTGFCRSPQETRKSSPTGSVASSVSNVMLTPSRQLTDAEKLRKVILELVDTEKSYVKHLNNLLEYYLEPLKRETFLSNAEITALFGNIQEIVTFQRQFLNNLEEALDLEPDFHQLEHPSQFKNVLFAIGSAFLYYVNHFKLYSSFCASHSKAQKVLHPNEGNQALQEFLAARNPKQQHSSTLESFLIKPIQRILKYPLLLQQLRNLTDPNAAEHLHLVEALKGMEKVAEHINEMQRIHEEYGAIFDHLFRQHQKSCKQPIDLSPGSDLLYYGGVEWLNISDFLGKIKKGLELHAMCFVFKSAVVFLCKERLRQKKKLMGVSSKNATNEVEIIRYQVLIPVTEVQVRASSAKDMDSHFLWELIHLRSQLQRRSEKVYVLSNSTAEFRNAFLKTIRQIIRESVRNMSIPSLKSMGGSTNSMTGVGNSSSGNSQTLERPKQQVSPQIMQGSQTLGKPKKSKINQRHSSGNIDYDNTTSGSQEADDPPVPQYSGQFRMRSKTVGDVGEQVAACRRNMNEREQEKPDPGTKSEGEDDSQQGTVKPKSSLGKTPNHLTLSTTSTLSVGSTGSQARLIQSSHPPATYHPVLMKDLGKRLSPSSDKSVVEMFDPTKGAAGATTAERPKPTQTDHQVDSAMDKHLKNLNLDSLIQTDIVEYMENLKEQHYEREQTGLPTTGSLAPSQPGSVTPAVGASPLPTRARSPATPHDTTHTTAHQTQPTATASHPTSPPQIAYSSTTPKAQAEEPNQCALKSSTDSSDSAVAPPPEMSSSSGSGKSDDSCSERAGEKAPGKERLSSSEGDGGSQGDQRYHTLSASSANSSPDGTLSPKPEKTKSPKRMKSVEREKSPIRIIKIKSPRNSIDIERHVRIEKSPDRARRASSPSAAAGAGATAATSNKKPPEGGILKKKQPNGGGVVGGILKRSVSPSVGSKPSESSDSTASKTDANLSSDYEQYQTATGNRCYLSLSSEYLGVEKDYPHIYQNATRDEKVRRAKRSASVGSTERMRSVEGYESSCGYSPNTSFDASSVEGSKKRYSISSERSTGEYRKQGSYSRSSSPLNVYSRDVSPNRCYARGLSPQTSLTRGLSLSPQSSFKGISPQSSFSRGPSPQRGYNSRGPSPQRSSGHASRGASPMRVGGAYYRGSTSPHSYGRGLSPQQSYSSRGPSPTRNNYPRGLSPQRRGSRHYSPPKEHHSSRGPVSPQRFYSRVPSPERHYERARGPLPLPKSHSRGASPQRTYRLTSSRSIDSSSFLDYDQNPNLHYARIEHDRRAYQQQHQQQLQQQHQHHLQQQQYASQSTHASCMECIMQDNMKPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VRS5</accession>
    <name>A0A6E8VRS5_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="8">PID domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ced-6 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040221383.1">
        <property type="nucleotide sequence ID" value="XM_040365449.2"/>
    </dbReference>
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        <property type="gene ID" value="ACON006921"/>
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    <dbReference type="CTD" id="35971"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012212"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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            <end position="200"/>
        </location>
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            <begin position="9"/>
            <end position="22"/>
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        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
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    <sequence length="493" mass="53243" checksum="4EA04C743CCADB43" modified="2020-08-12" version="1">MASTLMFWNKQNSNSNQNGNDAKNTKNGRNWLHAPDVLVNGHVAYLVKYLGSTPVEQPKGIEVVKEAIRRLQFTQQMKKAEGGGNVKTKKVEITISVDGVAIQEPRSLTIMHQFPLHKISYCADEKGVKKFFSFIAKTGTGATPTSSIASSGDDTNSSNNSTTSNGTEDRHECFVFISNKLASDITLTIGQAFDLAYRRYVSDSGKSLESVKLLAQKKQLEHTIAAYRQRLRDLSELVSKSDLDKLLLKMGIRDICDVPALENGVDGHNNYTSNNNNINSNNHNVNGTKTPDLGIDVSLPNNDDQLLIETSPKHFAPIVPPRNGIQNQINNTLEAFKPSVGTKLEGLLLNSDSDSDFDPRAPDTDTSISTGGGNKISNDLFGFEPPKPTSATLGQQLFGSVTNNGHNNGFTNGNGAVTNGFGAPNSPPPMLAPPPAKSAPRRTVPQTNGTTNGTSAYQDLFGSAPFNPQGNDTTKNGFFSSDDVLDTFDPLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VS11</accession>
    <name>A0A6E8VS11_ANOCL</name>
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        <submittedName>
            <fullName evidence="2">MIT domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VS11"/>
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        <property type="gene ID" value="ACON006789"/>
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        <property type="component" value="Unplaced"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="MITD1_C_sf"/>
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        <property type="entry name" value="MIT DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MIT DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16565">
        <property type="entry name" value="MIT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MITD1 C-terminal phospholipase D-like" evidence="1">
        <location>
            <begin position="32"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16565"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblMetazoa" id="ACON006789-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="180" mass="20989" checksum="EFAF7D050BADC5AB" modified="2020-08-12" version="1">MNRAEQVKELVTRWKSKGVISDRIHIVEGATGYSYGRIFGKYFNDEVHEILLEEPYVREHHQICNLVMFCELAVNSCRNLKYIQLATVKEAKNGDEQGRAFEVLKQSLHKQAVKFVVEYSEHMHDRQVILSNGYVIKIGRGLNYFKPSPSKYCLGAFNYHFRECRETNADVFYCPENNKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VTT1</accession>
    <name>A0A6E8VTT1_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">SPARC related modular calcium binding 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
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                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VTT1"/>
    <dbReference type="EnsemblMetazoa" id="ACON007489-RB">
        <property type="protein sequence ID" value="ACON007489-PB"/>
        <property type="gene ID" value="ACON007489"/>
    </dbReference>
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    <dbReference type="VEuPathDB" id="VectorBase:ACON007489"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041591"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="EFh_SPARC_SMOC"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="KAZAL_FS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00191">
        <property type="entry name" value="TY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.800.10:FF:000004">
        <property type="entry name" value="SPARC-related modular calcium-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.800.10">
        <property type="entry name" value="Thyroglobulin type-1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051950">
        <property type="entry name" value="Dev_reg/Prot_inhib"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002350">
        <property type="entry name" value="Kazal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019577">
        <property type="entry name" value="SPARC/Testican_Ca-bd-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000716">
        <property type="entry name" value="Thyroglobulin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036857">
        <property type="entry name" value="Thyroglobulin_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12352:SF30">
        <property type="entry name" value="FI05255P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12352">
        <property type="entry name" value="SECRETED MODULAR CALCIUM-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07648">
        <property type="entry name" value="Kazal_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10591">
        <property type="entry name" value="SPARC_Ca_bdg"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00086">
        <property type="entry name" value="Thyroglobulin_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00280">
        <property type="entry name" value="KAZAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00211">
        <property type="entry name" value="TY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57610">
        <property type="entry name" value="Thyroglobulin type-1 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51465">
        <property type="entry name" value="KAZAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00484">
        <property type="entry name" value="THYROGLOBULIN_1_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51162">
        <property type="entry name" value="THYROGLOBULIN_1_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="6 8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026221043" description="SPARC related modular calcium binding 2" evidence="10">
        <location>
            <begin position="25"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="domain" description="Kazal-like" evidence="13">
        <location>
            <begin position="30"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="Thyroglobulin type-1" evidence="12">
        <location>
            <begin position="60"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="11">
        <location>
            <begin position="206"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="Thyroglobulin type-1" evidence="12">
        <location>
            <begin position="302"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="151"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="504"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="507"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="130"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="339"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007489-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="543" mass="60604" checksum="FFB4B80F644F5329" modified="2020-08-12" version="1">MWLAPVCCVTVGLLLLGSLQTAGAIPPKLEPTISECAAKGGECDESKGRPVCGSDNKTYPTRCHLIRAQCSGHQVAFKHRGSCKDVCIASRTYALQQRASSPYTVKYVPRCREDGTYAPVQCIDGGGCWCVNGQGKQLPNTMVQHGKPICVKKGKSNQRRSSPRNPIRNKRSCSGLDRAVFNTNLLKLFQNEHVRVHQHNLTGPINEKTVLDWKFSVMDLNGNNLLDKTEYRTMKRLIKKVVKPKRCGRSFGKNCDADQDERLSRNEWYNCLAKDDVTLLQGKTMVDPKGEDDAIFKDSEADSDCLSDRKYALDEQKYGTNALYVPECTPDGRYQRVQCYRSTGYCWCVNEDTGKNIPGTSTKDEKPVCDQYASSTRPMKGCPEEKKVEFLKDLKAFLSKQVTTSFAGKIIPVWNTEEEKIAILSFVLLDKNQNKQLERKEWKAFRELVTATRQLRKCGKKMPRYCDVNSDRKITLSEWMSCLDGKRNSSVDVAAMSAPLVQAEVKSPSSSSSSSSSSSSGSSSSTSSKFKGRNPLEFVLKSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VU57</accession>
    <name>A0A6E8VU57_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="13">SH3 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the calcium channel beta subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VU57"/>
    <dbReference type="EnsemblMetazoa" id="ACON008028-RC">
        <property type="protein sequence ID" value="ACON008028-PC"/>
        <property type="gene ID" value="ACON008028"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011326"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008028"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039976"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005891">
        <property type="term" value="C:voltage-gated calcium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005245">
        <property type="term" value="F:voltage-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11863">
        <property type="entry name" value="SH3_CACNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000023">
        <property type="entry name" value="Voltage-dependent L-type calcium channel subunit beta-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046937">
        <property type="entry name" value="CAB1-4_N_A-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000584">
        <property type="entry name" value="VDCC_L_bsu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11824">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12052">
        <property type="entry name" value="VGCC_beta4Aa_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01626">
        <property type="entry name" value="LCACHANNELB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00072">
        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="9" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="domain" description="SH3" evidence="12">
        <location>
            <begin position="84"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="42"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="165"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="427"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="42"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="165"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="427"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008028-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="470" mass="51530" checksum="66D73B4213E87024" modified="2020-08-12" version="1">MVQRASSDAPSVSGGHRESRGIGLGLGYTSFQSAGYNKLFTQGSADSNYSQPSSDLSLDEEKESLRREKERQALGQLEKARSKPVAFAVRTNVSYDGSLDDDSPVHGSAVSFEVGDFLHIKEKYDNNWWIGRLVKEGCEVGFIPSPVKLEHIRMQASAARSSKLYTSKGSSSSGNLGASGVPGAEPSRGSTPPTPDDSDSMGASRHGKTPLATPPTKEKRKPFFKKQETSSPYDVVPSMRPVVLVGPSLKGYEVTDMMQKALFDFLKHRFESRIIITRVQADISLAKRSLMNNPSKRAIMERSNSRSSCLAEVQAEIERIFELARTLQLVVLDCDTINHPSQLAKTSLAPTIVYLKIASSKVLQRLIKSRGKAQAKNLSVQMVAAEKLSQCPPEMFDVILDENQLEEACNHLAEYLEAYWRATHPPVRPTPSVPRPLPSQEASPSGEQPGRMGPPPPTVFNMTDVEDTLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VU68</accession>
    <name>A0A6E8VU68_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Laminin subunit alpha-1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
            <location evidence="1">Basement membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON007849-RA">
        <property type="protein sequence ID" value="ACON007849-PA"/>
        <property type="gene ID" value="ACON007849"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006453"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007849"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035872"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005604">
        <property type="term" value="C:basement membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030054">
        <property type="term" value="C:cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005201">
        <property type="term" value="F:extracellular matrix structural constituent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009887">
        <property type="term" value="P:animal organ morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009888">
        <property type="term" value="P:tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00055">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="CDD" id="cd00110">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:001165">
        <property type="entry name" value="AGAP007850-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000011">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000706">
        <property type="entry name" value="Heparan sulfate proteoglycan 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000074">
        <property type="entry name" value="Laminin subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000090">
        <property type="entry name" value="laminin subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000069">
        <property type="entry name" value="Laminin subunit alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000082">
        <property type="entry name" value="Laminin subunit alpha 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000242">
        <property type="entry name" value="Laminin subunit alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000512">
        <property type="entry name" value="Laminin subunit alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000574">
        <property type="entry name" value="laminin subunit alpha-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000154">
        <property type="entry name" value="laminin subunit alpha-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000065">
        <property type="entry name" value="Laminin subunit beta 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000561">
        <property type="entry name" value="Wing blister, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000219">
        <property type="entry name" value="Wing blister, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000223">
        <property type="entry name" value="Wing blister, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000177">
        <property type="entry name" value="Wing blister, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050440">
        <property type="entry name" value="Laminin/Netrin_ECM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001791">
        <property type="entry name" value="Laminin_G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000034">
        <property type="entry name" value="Laminin_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008211">
        <property type="entry name" value="Laminin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002049">
        <property type="entry name" value="LE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056863">
        <property type="entry name" value="LMN_ATRN_NET-like_EGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10574:SF428">
        <property type="entry name" value="LAMININ SUBUNIT ALPHA-1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10574">
        <property type="entry name" value="NETRIN/LAMININ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00053">
        <property type="entry name" value="EGF_laminin"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24973">
        <property type="entry name" value="EGF_LMN_ATRN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00052">
        <property type="entry name" value="Laminin_B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02210">
        <property type="entry name" value="Laminin_G_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00055">
        <property type="entry name" value="Laminin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00011">
        <property type="entry name" value="EGFLAMININ"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00180">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="SMART" id="SM00281">
        <property type="entry name" value="LamB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00282">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00136">
        <property type="entry name" value="LamNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01248">
        <property type="entry name" value="EGF_LAM_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50027">
        <property type="entry name" value="EGF_LAM_2"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50025">
        <property type="entry name" value="LAM_G_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51115">
        <property type="entry name" value="LAMININ_IVA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51117">
        <property type="entry name" value="LAMININ_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0084">Basement membrane</keyword>
    <keyword evidence="7" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="8 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 12" id="KW-0424">Laminin EGF-like domain</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Laminin N-terminal" evidence="18">
        <location>
            <begin position="37"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="16">
        <location>
            <begin position="413"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="16">
        <location>
            <begin position="457"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin IV type A" evidence="17">
        <location>
            <begin position="526"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="16">
        <location>
            <begin position="760"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="16">
        <location>
            <begin position="873"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="16">
        <location>
            <begin position="917"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="16">
        <location>
            <begin position="964"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="16">
        <location>
            <begin position="1015"/>
            <end position="1063"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="16">
        <location>
            <begin position="1064"/>
            <end position="1111"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="16">
        <location>
            <begin position="1159"/>
            <end position="1204"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="16">
        <location>
            <begin position="1205"/>
            <end position="1260"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin IV type A" evidence="17">
        <location>
            <begin position="1300"/>
            <end position="1492"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="16">
        <location>
            <begin position="1536"/>
            <end position="1582"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="16">
        <location>
            <begin position="1637"/>
            <end position="1684"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="15">
        <location>
            <begin position="2451"/>
            <end position="2635"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="15">
        <location>
            <begin position="2639"/>
            <end position="2852"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="2848"/>
            <end position="2869"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="1722"/>
            <end position="1795"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="1942"/>
            <end position="1969"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="433"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="476"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="780"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="890"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="917"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="919"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="938"/>
            <end position="947"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="964"/>
            <end position="976"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="966"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="985"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="1015"/>
            <end position="1027"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="1017"/>
            <end position="1034"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="1036"/>
            <end position="1045"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="1064"/>
            <end position="1076"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="1066"/>
            <end position="1083"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="1085"/>
            <end position="1094"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="1159"/>
            <end position="1171"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="1161"/>
            <end position="1178"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="1180"/>
            <end position="1189"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="1231"/>
            <end position="1240"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="1555"/>
            <end position="1564"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="1637"/>
            <end position="1649"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="1639"/>
            <end position="1656"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="1658"/>
            <end position="1667"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007849-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="21">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2869" mass="316882" checksum="80473F2A9DEDA956" modified="2020-08-12" version="1">MGQPRTLSVGCDETELKLLSGLTVSSATTDYEQQLPKASGLWPSFFNVALRATISVNATCGQTGREEYCRLAEGAGRSRGSQCGICDANNPDPEKRHPITNIVDGTNSWWQSPTLQKGAKNDRVTINLDLGQLYQIVFFTMRAAISPLPAAWVLEKSMDGRAYDAWQYFAADDTECRERFGLPAYTANYIFKSDTEVICSTQFSSLEPLENGEINLSIISGRPSEMITSPELQNFTLARYVRIRLLRMQSAEAQRFKTKCFMCTKSFSSRSFYTIRSLRIGGRCFCSGHAGKCKTMDNNIDNEPQCECVHNTCGANCDRCCPLYNQRPYRIGTPVAANKCEKCECHGHAKSCIYDKLVDEQHLSLSIRGKMSGGGVCQNCTHHTTGVNCERCLPGYYRPMDRLPNHPEPCLPCNCTSPGAVGECSPIGGECRCLEGFTGAQCTECLPGHSGDRCTRCDCDARGTLSGRECDERCSCKAHVVGERCDRCDEGYFALDEDHIDGCLKCYCSGVGTKCSVAHVENSSYTTLKGWTVTNLAMSEHIVPTKDNDTGFLVFGMFEMPETEAVYWKAPEGYLGNLLRSYGSQLTFKMDWITVRGDTSGKPTVGPSLILVGRNGMKIAYGEESYEETGGATITVPLKEDSWYHVPRTVKDIITRLRRTEYHGDPVTRSQFMAVLTDVEVVLLRGTYHTDQVESVLEQADLFSGSHSTTDSSASSSGFIELCECPEGYRGTSCEQCAFGYVRIYETSVTHERVGRCIPCSMCNGHAASCDLETGECGLCLHNTVGTNCERCLPGFYGNATLGRPDDCKRCACPLESPSNNFSPNCQSRGGSSDPSEYVCTQCPEGYTGDHCELCDDGYYGEPTVIGGQCLPCPCHGGPCNAQTGECIECVGNTEGWRCERCKTGYWGVPDDGCEPCSCSELGALENVCDVTTGQCICKPRYGGRRCDECDVGYGNLDLDCPACACNVNGSASTVCNVVSGQCECNVGTEGIHCDRCREEFFGLSDEQPGACEGCNCHPKGSLGQGCNQKTGQCHCKPNIEGRQCNLCRKGYWDLNSGNGCIPCSCDTNGSELEGCDLHTGQCFCKTGVTGTSCDRCDVGFYGFSAQGCKRCDVCSSAAYVCDPDTGRCICPANSHGPECRSCIANTWGNEFQKGCKHCACDIVGSIGQSCDRETGQCSCKEGYTGRQCNECALGYYGYPTCHRCACDERGTLPSHNGTVFPCDRNGQCQCKEMVYGKRCDICRQGTFGLAAFHPEGCTRCFCFGRASECTQSDHSWGQVRLAGSRNLSVEYLERQDGHTEVDYVVILQLEGTQMHREDVNITSMNNLELIPSSSGNVSIGAYATFRYPLYFQLPPQFLGDRTASYGGLLNFTLITDGATINIPEPSLRQFPLVQLHTHENLVLDFYEQTIRYGQPVESHSVRLLGSLWRNHYDGAWANRTILMTALQNVRHIFVRGTTTMDFQQVVLTNVTLDTGIFVAGAENNHAYGVERCSCTPKYSGLSCQNPGPGYYRHRLPIVDLEWATIDDMIGKVVPCGCNGRSEECDPETGVCLNCRNNTGGEHCERCAEGFYGDPNLGQCSPCPCPETRKNFARGCTVRGSDVHCICKTGYTGALCDSCMRGYFGHPHLDNGHCEACECNREGSMSDECDHFTGECHCRPGITGRKCDRCEQPKHIVQDYRCKICDNCTITLIDDFEILASRLAEETTHIDRNGIPAPWVKLTKYEAKTRKLSNRVARLLEAEDMAKKFMADFAEPLTRNATRLTKRLQKMDNRLTAREEEIDKCNERANDLFEEINAIDGDLRNTIQILKNYGVGDHHIKLPLAVKEAKDLLADIRDRAEAVEFDDTVLECANKQFDHWSNVSKKVERQARRLDDLKFEIEQIHHKADHLQNYTVQVFRHVQETEVYQTTNQKHFDKARAAYEKALEDEAEVMKLLDTNIMSDTDVTLEQLGDNYDQLEKDNRTLQFLLDGLSDATEDLIREKSILLETQIPQALRHAEQLKAKADHIKNKFQTTEDASAKAMKASQAYENIISAIVSAQSSADNATSMVEKADQLIHPLHDVTISDQSRKALGVSTNLTSRANKELNKAVALNETMIQKEKSVQSLKDQIWQTAIKNNNLMDELGKVERGETMKTVQSDLDRSSIVSEQMKFVRQDAINLNSDVYKLKLKLKSLEPEWDTKFGMAEENVSQTFGNIRKAKDKLNGIEALGKIQDERFQAWNQSFSGKLQQLRDQIALAKHAAEGIRVSMESADQCIRSYAPATFGPSTTNRIVMSIALTNPKVQNSPLAYIEGDDRRFIALELRQRKIRMLWSLDGESVTTMTHPQEIEVRDLKNDAAWYFIDATRTFNVGTLNVRHMLPAGVLSNARPVTGASSPAYSTINIGPSRRIWVGGIPDDLRVPELEPSQGLGVAVSQLYVDQRQLGLWHFTSSSGKCAGAMLGPQEVASSTNERNFNGNGYAVLQHTGRLNKVEFSLSLSFKTLDEDALIFLALDEKNNRSVSLTLYQGRLVFRVDYGDDARLEINTTKRYNTGRWVVVEASRQYRKSRTDEGVLKVDGLDNILGSPTKPIGASMLPVLSDKYYLGGVPPGFKTGTTKAPGADHAFLGCVKGLQISGTSYDPLDSTTHFGIETSCDSTIVKAGFFGDGYVEFPSFPLKKRANFGFVFRTMEPDALLLLSAYPSRIHDDYDAKDTFGNYSVFLSEGRLHLWMDAGTGRVELTSNGTLNDGEYHVLAVVKQGRHVELRIDDKLQMSRTLQSSLVNMPGESGGLYIGGVPYDPAFASLAKVFDGLKGVVANVVFNNQTLSFGQALNFTDVQMGRTGPPMGSQGLYTALMKTEPIGRSFKAAPEGCHRSVENATNSKEEADDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VUC7</accession>
    <name>A0A6E8VUC7_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ras-related protein Rab</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">The small GTPases Rab are key regulators in vesicle trafficking.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Membrane</location>
            <topology evidence="6">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_040385239.2"/>
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        <property type="protein sequence ID" value="ACON007654-PD"/>
        <property type="gene ID" value="ACON007654"/>
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    <dbReference type="CTD" id="10981"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004658"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007654"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:late endosome"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:GTPase activity"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="P:endosome to lysosome transport"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:phagosome-lysosome fusion"/>
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        <property type="entry name" value="Rab32_Rab38"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RAB32, member RAS oncogene family"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030697">
        <property type="entry name" value="Rab29/Rab38/Rab32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47981">
        <property type="entry name" value="RAB FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47981:SF39">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 6" id="KW-0636">Prenylation</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007654-PD"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="215" mass="24204" checksum="2F6D36904BF53193" modified="2020-08-12" version="1">MATGQAEKREHLYKILVIGELGTGKTSFIKRYVHQFFSQNYRATIGVDFALKVLNWDQNTIIRLQLWDIAGQERFGNMTRVYYKEAVGAFIVFDVTRSATFDAVIKWKNDLDSKVQLPDGKPIPCILLANKSDQQKQGIVTTPAKLDEYVKEHGFAGWFETSAKENVNIEEAAKSLVNKILMNDKLLNTGEVIDSERFALVGGKSDTSQKKSCSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VUD2</accession>
    <name>A0A6E8VUD2_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="4">NRF domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VUD2"/>
    <dbReference type="EnsemblMetazoa" id="ACON007659-RA">
        <property type="protein sequence ID" value="ACON007659-PA"/>
        <property type="gene ID" value="ACON007659"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_014118"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007659"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032642"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002656">
        <property type="entry name" value="Acyl_transf_3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006621">
        <property type="entry name" value="Nose-resist-to-fluoxetine_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052728">
        <property type="entry name" value="O2_lipid_transport_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11161:SF15">
        <property type="entry name" value="GH19286P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11161">
        <property type="entry name" value="O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01757">
        <property type="entry name" value="Acyl_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20146">
        <property type="entry name" value="NRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00703">
        <property type="entry name" value="NRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026302041" evidence="2">
        <location>
            <begin position="24"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="348"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="433"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="477"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="530"/>
            <end position="551"/>
        </location>
    </feature>
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        <location>
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            <end position="613"/>
        </location>
    </feature>
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        <location>
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            <end position="641"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="661"/>
            <end position="680"/>
        </location>
    </feature>
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        <location>
            <begin position="692"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="731"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="765"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="domain" description="Nose resistant-to-fluoxetine protein N-terminal" evidence="3">
        <location>
            <begin position="184"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00703"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007659-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="810" mass="91461" checksum="BFD65A3FB0CFB040" modified="2020-08-12" version="1">MLFKRLRCIICVATVLFVGLTLTTTPVAAGSADEDGRNTNKVVNATDAQPLASCNATSDTGTTGCPLAVGAASGVTQPLRGKLNNGTCVSAYCGSDESNSSSSSVEEMDDDVINATSIGKGGHSQQQQQQQHRSHLLPSTLLGADEDDAALMYGGKKVVLKAEVLKHTSVLYGLISIAERDELSERCFRELQRVYHGIQQKEIWAMKVLDASGSQEPGFMWGHNHWIGSEKGCESTRTPLSITLSDRYRRNMKRNLVSALAPFELDYRIVFAKHQSAWQVDVKFQSENILHIGVCIPRSCTNDELHNLTARYFDESLVLAQDIYEFKPNVLHVKNTTLDRAFWSKPSVFIIGVSLAFTLLMIYLAHSREQRLMEEKNNNHMDGTAGASPAAARTEGGSDLADASTFTERVIDSFNLRKNLNTIFRTDSGPQSLPVICGMKSICCFLILCFHMQWYTFYTVHNPSVMLHYAEQIRFQLVSNAPLLVDVFFAISGFLLSYNFIRNRSKVQEVKANTVWENGKLYGKMLLHRYLRLSPMYLFVTVLGELTHSYIVETSKFWVHERSDLGCQRYWWRNLFYIQNLYPVDDFCLTWTWSLACEMQYYMLFTVLLFVYAKSAKFGKKVFGAFAIGTLLFNIALTLNHRFIPSYDVLYNTGDALYTAPWSRVSPYVVGVYFGWLMASSRDKFTITQNQYRWYWLLACFCVIFSMHSTIKRNFPFPIASTVMVAVRWLISWAACWVILATGCGYSNIVTRVLSSKFFVHINKLTYGIYLLNPLIIIVTFGVSDSSVHADPIPAVSYFNLHLFLLSSTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VVX2</accession>
    <name>A0A6E8VVX2_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="7">UBC core domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VVX2"/>
    <dbReference type="EnsemblMetazoa" id="ACON008680-RA">
        <property type="protein sequence ID" value="ACON008680-PA"/>
        <property type="gene ID" value="ACON008680"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON008680-RB">
        <property type="protein sequence ID" value="ACON008680-PB"/>
        <property type="gene ID" value="ACON008680"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON008680-RC">
        <property type="protein sequence ID" value="ACON008680-PC"/>
        <property type="gene ID" value="ACON008680"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011768"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008680"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_036611"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000080">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 R1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="6">
        <location>
            <begin position="1"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="134"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="148"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="3">
        <location>
            <position position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008680-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="178" mass="20180" checksum="8EA7B631A03A339F" modified="2020-08-12" version="1">MKFPPDYPYSPPSIRFLTKVWHPNVYENGDLCISILHPPVDDPQSGELPCERWNPTQNVRTILLSVISLLNEPNTYSPANVDASVMYRRWRDSHGKDNEYPNIIRRQAQAAKAEAEKEGIIVPLTLEEYCIKAKTKPTGQEPQLDMTDFYDDDYDLDTEDDDNASVESDGDDSGNGES</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VVY1</accession>
    <name>A0A6E8VVY1_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VVY1"/>
    <dbReference type="EnsemblMetazoa" id="ACON008688-RB">
        <property type="protein sequence ID" value="ACON008688-PB"/>
        <property type="gene ID" value="ACON008688"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008688"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008688-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="116" mass="13304" checksum="C3D1EF1B7FC0424A" modified="2020-08-12" version="1">GKPTIRQQPPLLRDCLFILVRTRDKRKAKLIYRIECEARFWDRIALCPSNKIDRHTRAKKCRSVTMCVCVSRLTVRTCRPFSPEAISLIKRDRATERHYCSLVTTGSGCGVAAPQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VWG4</accession>
    <name>A0A6E8VWG4_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">LUC7</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Luc7 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040237123.1">
        <property type="nucleotide sequence ID" value="XM_040381189.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040237124.1">
        <property type="nucleotide sequence ID" value="XM_040381190.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VWG4"/>
    <dbReference type="EnsemblMetazoa" id="ACON008314-RA">
        <property type="protein sequence ID" value="ACON008314-PA"/>
        <property type="gene ID" value="ACON008314"/>
    </dbReference>
    <dbReference type="GeneID" id="120958407"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_008265"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008314"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032564"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006376">
        <property type="term" value="P:mRNA splice site recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004882">
        <property type="entry name" value="Luc7-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12375">
        <property type="entry name" value="RNA-BINDING PROTEIN LUC7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03194">
        <property type="entry name" value="LUC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="179"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="41"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="179"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="283"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008314-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="296" mass="34741" checksum="72A1EC4EDD348EC7" modified="2020-08-12" version="1">MDLGECPKVHDLALRADYENASKNKDYYYDVDAMEHLQAFIADCDRRTEAAKKRLAETQEELTAEVAAKANAVHELAEEIGKKLAKAEALGEAGQVEESMKLMSEIEELRSEKTRAEQEYRSSIPASTYQQQKLRVCEVCSAYLGIHDNDIRLADHFGGKLHLGFLAIREKLAELEKTAGPRQKELRKTGRDRDHEDRSRSRYVGGRELDRRSRALARSRDRKDTNREIKPGDDRKDRNEGRDRDRDQRDRGDRRRSPDRSSRRSRSRDRSRERDRHNDRHNDRRRRSRSRERSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VWG6</accession>
    <name>A0A6E8VWG6_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 20">Polypeptide N-acetylgalactosaminyltransferase</fullName>
            <ecNumber evidence="20">2.4.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Protein-UDP acetylgalactosaminyltransferase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="23">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-seryl-[protein] + UDP-N-acetyl-alpha-D-galactosamine = a 3-O-[N-acetyl-alpha-D-galactosaminyl]-L-seryl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23956"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:12788"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:53604"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:67138"/>
            <dbReference type="EC" id="2.4.1.41"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-threonyl-[protein] + UDP-N-acetyl-alpha-D-galactosamine = a 3-O-[N-acetyl-alpha-D-galactosaminyl]-L-threonyl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52424"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11689"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:67138"/>
            <dbReference type="ChEBI" id="CHEBI:87075"/>
            <dbReference type="EC" id="2.4.1.41"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 20">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 20">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 20">Golgi apparatus membrane</location>
            <topology evidence="2 20">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 20">Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="20"/>
    <dbReference type="RefSeq" id="XP_040230806.2">
        <property type="nucleotide sequence ID" value="XM_040374872.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VWG6"/>
    <dbReference type="EnsemblMetazoa" id="ACON008613-RA">
        <property type="protein sequence ID" value="ACON008613-PA"/>
        <property type="gene ID" value="ACON008613"/>
    </dbReference>
    <dbReference type="GeneID" id="120954714"/>
    <dbReference type="KEGG" id="acoz:120954714"/>
    <dbReference type="CTD" id="326151"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006891"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008613"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033965"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004653">
        <property type="term" value="F:polypeptide N-acetylgalactosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd23433">
        <property type="entry name" value="beta-trefoil_Ricin_GALNT1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02510">
        <property type="entry name" value="pp-GalNAc-T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000047">
        <property type="entry name" value="Polypeptide N-acetylgalactosaminyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000005">
        <property type="entry name" value="Polypeptide N-acetylgalactosaminyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045885">
        <property type="entry name" value="GalNAc-T"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675">
        <property type="entry name" value="N-ACETYLGALACTOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675:SF101">
        <property type="entry name" value="POLYPEPTIDE N-ACETYLGALACTOSAMINYLTRANSFERASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00652">
        <property type="entry name" value="Ricin_B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00458">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="Ricin B-like lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 20" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 20" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="13 20" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10 20" id="KW-0430">Lectin</keyword>
    <keyword evidence="17 20" id="KW-0464">Manganese</keyword>
    <keyword evidence="14 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="7 20" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="Ricin B lectin" evidence="22">
        <location>
            <begin position="516"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="92"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="21">
        <location>
            <begin position="102"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00050905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00052209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU361242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="SMART" id="SM00458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008613-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="657" mass="74786" checksum="E848941F41CE8455" modified="2020-08-12" version="1">MSVVSLNRMFRGKIRTNTCRIVVLTSLVWLLIDVILIVKYADCPSTGGGSSWLCKRNGDGYDVEVPNAARQSQQQRRAAAPEDDDPIVDAEEIDTNSNNFGDPGGYGGGGGGGGRDADSSMPRTYRPQELKKWRQAPTVAENYGRPGEMGKPVKIPANQQELMKEKFKENQFNLLASDMIWLNRSLTDVRHHDCKKKHYPAKLPTTSIVIVFHNEAWSTLLRTIWSVINRSPRPLLKEIILVDDASEREHLGRQLEEYVRTLPVPTFVLRTGKRSGLIRARLLGAKHVKGQVITFLDAHCECTEGWLEPLLARIVLDRKTVVCPIIDVISDETFEYVTASDQTWGGFNWKLNFRWYRVPAREMQRRNHDRTAPLRTPTMAGGLFSIDRDYFYEIGSYDEGMDIWGGENLEMSFRIWQCGGILEISPCSHVGHVFRDKSPYTFPGGVANIVLKNAARVAEVWLDEWKEFYYQMSPGARKASAGDVSERRALRERLKCKSFRWYLENIYPESQMPLDYYFLGEIRNVKTHNCLDTMGRKSNEKIGSSYCHGLGGNQVFAYTKRHQIMSDDNCLDASNALGPVNLVRCHGMGGNQEWIYDDEEKTIKHVNSGNCLTRASDDDPSTPLLRPCNYSEGQQWLMQSKFKWQAHHGDNRIGEDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VWG8</accession>
    <name>A0A6E8VWG8_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Tyrosine-protein kinase</fullName>
            <ecNumber evidence="13">2.7.10.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 13">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the protein kinase superfamily. Tyr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.2" evidence="13"/>
    <dbReference type="RefSeq" id="XP_040237121.1">
        <property type="nucleotide sequence ID" value="XM_040381187.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VWG8"/>
    <dbReference type="EnsemblMetazoa" id="ACON008317-RB">
        <property type="protein sequence ID" value="ACON008317-PB"/>
        <property type="gene ID" value="ACON008317"/>
    </dbReference>
    <dbReference type="GeneID" id="120958406"/>
    <dbReference type="CTD" id="124191448"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002310"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008317"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035633"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004715">
        <property type="term" value="F:non-membrane spanning protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048468">
        <property type="term" value="P:cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002009">
        <property type="term" value="P:morphogenesis of an epithelium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007424">
        <property type="term" value="P:open tracheal system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007435">
        <property type="term" value="P:salivary gland morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05059">
        <property type="entry name" value="PTKc_Tec_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09934">
        <property type="entry name" value="SH2_Tec_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11768">
        <property type="entry name" value="SH3_Tec_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000052">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000190">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000053">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000045">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050198">
        <property type="entry name" value="Non-receptor_tyrosine_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24418">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="1 11" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="3 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 13" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="SH3" evidence="16">
        <location>
            <begin position="200"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="15">
        <location>
            <begin position="268"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="384"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="11"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="31"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="142"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="412"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00051245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU362096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008317-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="644" mass="72315" checksum="B4AFA9F51B61B4AD" modified="2020-08-12" version="1">MTLLSVWRLGTGSSPAQNSTRSTSPNVPNGHLPLPQQQSQVHPQQQPQQQQLQHHSGTVPVLQAHQQQQQQPQPTQHPPQQHSQSHSPSLQHPAASQQQVHLQQQQAALQAALQQQQQPPLLQQQTSQQSSHHLNSSSSSLGNITPTFVAQPQSPTVNSFKTASLNGVGLHVNGGGDLNMPGGPTVTPGTPNTKAKDVSPRVKIVVALYPFKAIESGDLSLEKNAEYEVIDDSQEHWWKVKDQHGTIGYIPSNYVKEKELLGLQKYDWYVGDMSRQRAESLLKQNDKEGCFVVRKSSTKGLYTLSLHTKVPQSHVKHYHIKQNARSEFYLSEKHCCETIPDLINYHRHNSGGLACRLKTSPCDRPVPPTAGLSHDKWEIHPSELMILEELGSGQFGVVRRAKWRGSIDTAVKMMKVGTMSEDDFIEEAKVMTKLQHPNLVQLYGVCSKHRPIYIITEYMKHGSLLNYLRRHEQSLIGNMGLLLDMCIQVCKGMSYLERHNYIHRDLAARNCLVGSENTVKVADFGLARYVLDDQYTSSGGTKFPIKWAPPEVLNYTRFSSKSDVWAYGVLMWEVFTCGKMPYGRLKNTEVVERVQRGIILEKPKACAKEIYDVMKKCWSHSPEDRPGFRILKDQLAAVAQGLTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VWH8</accession>
    <name>A0A6E8VWH8_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Transporter</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VWH8"/>
    <dbReference type="EnsemblMetazoa" id="ACON008940-RB">
        <property type="protein sequence ID" value="ACON008940-PB"/>
        <property type="gene ID" value="ACON008940"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012420"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008940"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035641"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005283">
        <property type="term" value="F:amino acid:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005328">
        <property type="term" value="F:neurotransmitter:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005034">
        <property type="term" value="F:osmosensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0089718">
        <property type="term" value="P:amino acid import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042065">
        <property type="term" value="P:glial cell growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019226">
        <property type="term" value="P:transmission of nerve impulse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000175">
        <property type="entry name" value="Na/ntran_symport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002944">
        <property type="entry name" value="Na/ntran_symport_inebriated"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037272">
        <property type="entry name" value="SNS_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF037979">
        <property type="entry name" value="Na_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616:SF303">
        <property type="entry name" value="SODIUM- AND CHLORIDE-DEPENDENT GABA TRANSPORTER INE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616">
        <property type="entry name" value="SODIUM/CHLORIDE DEPENDENT TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00209">
        <property type="entry name" value="SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01205">
        <property type="entry name" value="INEBRIATED"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00176">
        <property type="entry name" value="NANEUSMPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161070">
        <property type="entry name" value="SNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00610">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50267">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0915">Sodium</keyword>
    <keyword evidence="5 10" id="KW-0769">Symport</keyword>
    <keyword evidence="4 10 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="99"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="129"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="171"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="260"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="292"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="372"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="431"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="476"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="507"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="548"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="590"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="681"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="681"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="713"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="346"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="378"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="443"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="447"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="211"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR600175-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR600175-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008940-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="725" mass="81374" checksum="0DF89E75063B0317" modified="2020-08-12" version="1">MSVHGGCKLEPTANGVERIEHHQPLKMPVQANGTPGSTDRTPLMTGIPSYPNSPVMMRRYIGDPPDETYENESYGGMSDDSDLDNLKPRKQHWANKMQFVLACIGYSVGLGNVWRFPYLCYKSGGGVFLVPYFIILLICGIPMLFMELAVGQYTGRGPIGALGQLCPLFKGTGLASVVVSFLMSTYYSVIIAYAIYYFFTSFRPELPWTDCSHRWNTPDCWIPERLKHNLTRPDMSRTPTEEFFENKVLQISHGIEYPGAMRWELVACLVCAWILVYFAIWKSIKSSAKVRYLTATLPFVLIVVFLGRSLTLEGADKGLHYFFRPNWEELGRANVWINAAAQNFNSIGIAFGSMISFASYNKYNNNILHDTLAVSFVNAITSLLVGIFAFATIGNIALEQNTTVENVISGGPGLIFVVYPQALAKMPAAQLWAVLFFFMLLCLGLNSQFAIVEVVVTSIQDGFPRWIKRKLVYHELLVLIVCVVSFFAGLPNLIQGGIYFFQLIDHYAASVSIMFLAFFETIAIAWFYGINRLSKNVKQMTGRYPSFYLRFCLLIAVPLLLISLWIFSLINYEAPTYHNGKYHYPGWAHGLGWTIASASLVCIPSYAVYQIVRAEGNTFGEKLLNTLKPNIYECKICGEHHCDHDFPDEELGPEMTIVESTSGAPLILQPPPPGHLQYTQLLQQQQHQQQHQQSQDEPMLRTTNTNSPPPTTNGPQNSNSNGDTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VWI0</accession>
    <name>A0A6E8VWI0_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>12-(9Z-hexadecenoyloxy)-octadecanoate + H2O = 12-hydroxyoctadecanoate + (9Z)-hexadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52072"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:32372"/>
            <dbReference type="ChEBI" id="CHEBI:84201"/>
            <dbReference type="ChEBI" id="CHEBI:136312"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:52073"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>12-(9Z-octadecenoyloxy)-octadecanoate + H2O = 12-hydroxyoctadecanoate + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52060"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:84201"/>
            <dbReference type="ChEBI" id="CHEBI:136302"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:52061"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>12-hexadecanoyloxy-octadecanoate + H2O = 12-hydroxyoctadecanoate + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52056"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:83677"/>
            <dbReference type="ChEBI" id="CHEBI:84201"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:52057"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>12-octadecanoyloxy-octadecanoate + H2O = 12-hydroxyoctadecanoate + octadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52080"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:25629"/>
            <dbReference type="ChEBI" id="CHEBI:84201"/>
            <dbReference type="ChEBI" id="CHEBI:136330"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:52081"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>13-(9Z-hexadecenoyloxy)-octadecanoate + H2O = 13-hydroxy-octadecanoate + (9Z)-hexadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52076"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:32372"/>
            <dbReference type="ChEBI" id="CHEBI:136304"/>
            <dbReference type="ChEBI" id="CHEBI:136315"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:52077"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>13-(9Z-octadecenoyloxy)-octadecanoate + H2O = 13-hydroxy-octadecanoate + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52064"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:136303"/>
            <dbReference type="ChEBI" id="CHEBI:136304"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:52065"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>13-octadecanoyloxy-octadecanoate + H2O = 13-hydroxy-octadecanoate + octadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52084"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:25629"/>
            <dbReference type="ChEBI" id="CHEBI:136304"/>
            <dbReference type="ChEBI" id="CHEBI:136335"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:52085"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>9-(9Z-hexadecenoyloxy)-octadecanoate + H2O = (9Z)-hexadecenoate + 9-hydroxy-octadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52068"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:32372"/>
            <dbReference type="ChEBI" id="CHEBI:136286"/>
            <dbReference type="ChEBI" id="CHEBI:136309"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:52069"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>9-(9Z-octadecenoyloxy)-octadecanoate + H2O = 9-hydroxy-octadecanoate + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52048"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:136282"/>
            <dbReference type="ChEBI" id="CHEBI:136286"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:52049"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>9-hexadecanoyloxy-octadecanoate + H2O = 9-hydroxy-octadecanoate + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52052"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:83670"/>
            <dbReference type="ChEBI" id="CHEBI:136286"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:52053"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>9-octadecanoyloxy-octadecanoate + H2O = 9-hydroxy-octadecanoate + octadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52096"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:25629"/>
            <dbReference type="ChEBI" id="CHEBI:136286"/>
            <dbReference type="ChEBI" id="CHEBI:136373"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:52097"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the AIG1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VWI0"/>
    <dbReference type="EnsemblMetazoa" id="ACON008626-RB">
        <property type="protein sequence ID" value="ACON008626-PB"/>
        <property type="gene ID" value="ACON008626"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009628"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008626"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_029259"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006838">
        <property type="entry name" value="ADTRP_AIG1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10989">
        <property type="entry name" value="ANDROGEN-INDUCED PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10989:SF16">
        <property type="entry name" value="AT02829P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04750">
        <property type="entry name" value="Far-17a_AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="59"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="101"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="142"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="172"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="208"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008626-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="253" mass="29037" checksum="AE68BD931AF796F6" modified="2020-08-12" version="1">MSSVQLYKLPAFVITWHLIAFGQYGYAIYFDRFFVHIPDDLDMPKMMRPTEYGGRSKFLTYWCLILQAVYFTICLLNDFIGTNEVAPKRMPLIRKLKDYMLAAFAFPVALNVGVTFWTLMAIDRELVFPKALDAVFPGWLNHVMHTNIVIFMVIEICTSFRQYPSRKAGLTGLGIFMASYLGWLHVVRHFGGIWVYPVLDVLNLPQRILFFAVSLGFCVALYLLGEFFNNLIWTKELKQLRAATAGGASKKTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VYI0</accession>
    <name>A0A6E8VYI0_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Sodium/hydrogen exchanger</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Recycling endosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VYI0"/>
    <dbReference type="EnsemblMetazoa" id="ACON009036-RA">
        <property type="protein sequence ID" value="ACON009036-PA"/>
        <property type="gene ID" value="ACON009036"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001641"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON009036"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041466"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055038">
        <property type="term" value="C:recycling endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015386">
        <property type="term" value="F:potassium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015385">
        <property type="term" value="F:sodium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051453">
        <property type="term" value="P:regulation of intracellular pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098719">
        <property type="term" value="P:sodium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018422">
        <property type="entry name" value="Cation/H_exchanger_CPA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018410">
        <property type="entry name" value="Na/H_exchanger_3/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004709">
        <property type="entry name" value="NaH_exchanger"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00840">
        <property type="entry name" value="b_cpa1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10110">
        <property type="entry name" value="SODIUM/HYDROGEN EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10110:SF98">
        <property type="entry name" value="SODIUM_HYDROGEN EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01084">
        <property type="entry name" value="NAHEXCHNGR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01087">
        <property type="entry name" value="NAHEXCHNGR3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 13" id="KW-0050">Antiport</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0967">Endosome</keyword>
    <keyword evidence="10 13" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0915">Sodium</keyword>
    <keyword evidence="12 13" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="6 13 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 13" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="275"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="304"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="332"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="365"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="395"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="439"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="556"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="600"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="624"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="16">
        <location>
            <begin position="251"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="194"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="840"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="888"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1081"/>
            <end position="1219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="213"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="914"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="1165"/>
            <end position="1174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1175"/>
            <end position="1186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="1194"/>
            <end position="1213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009036-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1219" mass="134030" checksum="5FDD5079F475D075" modified="2020-08-12" version="1">MAVTLKQEVNLSRRACRPTTTNNDDCLQEQRTLLTTPTEEGSWHNDTCYGGGGANSRLVTVPAPAKELTDSSRSGGLPSSSSLSCLLATIVMWCTGQVLLKLRQAGQGGWREVQQPPRLSRLCIYVVFCSALLSHNAFVLARPNLSAPASGEKVPVGQLSPLDLAFDASSGSLTSASQLKRSAEAAAIIASTAPSADGGAGHEAEDTALGHGRVGEGEGGEHGGEHVVERYPVSQVDFSRVETPFVIGVWILSASIAKIGFHMTPKLSRIFPESCLLIVVGVIIGVLLRYATNLHVSPLTPNTFFFYMLPPIILDAGYFMPNRMFFDNIGTILLMAVIGTIFNIATIGGSLWACGQTGIFGVDLPFLHIFLFSSLIAAVDPVAVLAVFEEIHVNEVLYIVVFGESLLNDAVTVVMYHMFEVYNEIGASEIIAVDIVSGIASFFVVALGGTIIGVIWGFLTGLVTRFTDHVRVIEPIFIFVMAYLAYLNAEIFHMSGILAITFCGITMKNYVEQNVSHKSHTTIKYALKMLSSSAETIIFMFLGVATVNNKHVWNTWFVLLTIIFCSVYRILGVLILSALANRFRIHKLTKVDQFVMSYGGLRGAVAFALVLLVDVNHIPLQPMFLTTTIAVVYFTVFLQGITIKPLVKILNVKRSSKRKPTMNERIHERFMDHMMAGIEDIVGKTGNYNIRDKFKRFDNRFIRPLLIKNLQGPEPKILETYSKLTMRDAMDYMRRNPSTIGQMSGTESMSALFRNYTSYFGGSPSFTNLENSTRNLDMQELDYNPSKKDLTDAKIHHLLSEELKPYRRHRRLSYSRHAVDDRDLSTQVNYKMQMNIRRMISEKKHHKRSKRVKDGKQQNHVSFPEFVQNGSAKQFANDYINEVLHEDNEDEERQAAGPSEDWDGGGLTFTAKSSLDERGAAKEDKRFSRESRFLESEQRVATPTAIESVLPWKRVDESDDNGAIKQNEFPSWASNKEYLAYNSPSATFLGGLTQPKQAKSVIGLFRRESSGSGGVVIGGVTDSETSTPVAATTPVGRMTAVPKDKRSASIAGYITIDGGDTAGNPMLDPLGISRAGPHATAFPVTASHRRNNRRGSMLELSGEAIPEENYTKSLPERDKYGARRAPALIKASSTNTPKSAGRRSGDGGGPVNPQTTALLSASSTDSDEDDEEEGSGDRHRADDATRRLNGAGNNDDEDEDDDEDDDDEDEDCADEQHPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VYI8</accession>
    <name>A0A6E8VYI8_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="3">BHLH domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="EnsemblMetazoa" id="ACON009664-RB">
        <property type="protein sequence ID" value="ACON009664-PB"/>
        <property type="gene ID" value="ACON009664"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_008281"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON009664"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037993"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016922">
        <property type="term" value="F:nuclear receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032870">
        <property type="term" value="P:cellular response to hormone stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000145">
        <property type="entry name" value="AGAP009664-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000080">
        <property type="entry name" value="Taiman isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056193">
        <property type="entry name" value="bHLH_NCOA1-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017426">
        <property type="entry name" value="Nuclear_rcpt_coactivator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10684">
        <property type="entry name" value="NUCLEAR RECEPTOR COACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10684:SF4">
        <property type="entry name" value="TAIMAN, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23172">
        <property type="entry name" value="bHLH_NCOA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14598">
        <property type="entry name" value="PAS_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BHLH" evidence="2">
        <location>
            <begin position="154"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="559"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="751"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="797"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="972"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1023"/>
            <end position="1051"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1125"/>
            <end position="1219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1323"/>
            <end position="1441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1501"/>
            <end position="1606"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1636"/>
            <end position="1701"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1714"/>
            <end position="1742"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1762"/>
            <end position="1820"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1836"/>
            <end position="1887"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="559"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="757"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="797"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="809"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="826"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="868"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="884"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1024"/>
            <end position="1051"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1125"/>
            <end position="1173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1195"/>
            <end position="1219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1347"/>
            <end position="1356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1357"/>
            <end position="1396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1397"/>
            <end position="1410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1510"/>
            <end position="1522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1529"/>
            <end position="1543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1582"/>
            <end position="1606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1658"/>
            <end position="1675"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1714"/>
            <end position="1727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1776"/>
            <end position="1809"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1861"/>
            <end position="1875"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009664-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1972" mass="207691" checksum="D4BE117F277A5D24" modified="2020-08-12" version="1">MSRPCRIASVATVPVNYRLGPCELPLPDQWLVHTQLSQSTQSSQASFYAASQSGGQSLGGLGGLSTAATLPIQQHSSSSSNQQHQQNHQQQQQQPPSLPSQLQNQITAGAVTPGAAPASQLQSQSKIMNVVVPSVACPPTASKKIRRKPDAKPQSQINKCNNEKRRRELENEYIEQLGEFLQIKRDMTACKPDKAAILSEVVRTFRHMLEQGNPNLTGSRCSKCSPDCSASCKLHPVQQGEVSSTEPPLPEPSVNGHSPEKSAYFEAVQHYISNVGWALLEINSEGVIECATENVRDVLHYSRTELHGQSIYSYLHTGDHSKLSPILNKNSFELNWDQNEMFYQTPKRTIRTKIRWLLRAPEGANETIEQKQQRLEKYKDLLIISAPVKDDTEESSSVLCLITLPEDDQATIETTTMPQTLDEQLTLKLDTSGNIIDYNSSTLRKQFAGNLTKESIRSIYDLCHYQDRPRLNEHLGNVRSSNGTPHELTYRLRLGGPDVYVHVKTQSKLFRCTKPNETDFIMAICTVLTENEVAMLSADSGAMAMGVGGSSSGGLSAAMSGSNINNNNGNSSSSSNPTSSSSSLGLLMPSTSGTVTGGGLNGSNDVVARHMAQITQGVSNMGGPLMSSVLNGGGGSTVGTGSNASGMLGGGGMTGGSMSVAGGGMAGPGGTTPGSAGGSGLGGGGGMGAGAGGMGPSAGSMGSIVSPRSNPNSSSLLCGPNSDSSNFFNTEFELDFPHSTFDMEAVGVGWDSRPDSRTSVTPVSTPRPPSVSAYSPAAAPMCASPMTPYYSGSTMGGMPSPSNNNNNGVPGGGGGGSSGSSGGGLMNPSLSLNNNNNNTNNNNTGTPFGTNAFQFPFEDSKDKLQDLQQQQQQQQQQQHHHPHMSPMQQHQSAMSAQQQQMVQRQQHHQQQQQQQHLQQHLQQQSQPPSNTHDSERLRNLLTTKRPHSNASSSSGLDMDHDHRNPNRILKLLNDKSDDDESDARNRQGPSELLKQLQKVKDEPKEHNPPPLNNEELIQMLRVQSNDRKRPSTEPDEGAAVKRTDNRPSKLRERNKMLASLLANPAKAPMPMQTAMPFNRIIPDIPNSGLARQLTNVSSSTAPNQTLNNNNLTTSNNNLKQVQQLNQMRLQQQQQQQQQQQQQQMRKSAMPSQSQQPPTSSDIYLNHQQQQQQQQHHHQQHPQTHPQHLQQHHAQQHQQQHHAQASMLQQQQMLAAQRQQNFSPAIQDAGIGSAGGGSFATATPATSTQLVSEWDPELNEILNHVIEIAPEGDFAESELNSILGLSPIESSTSSVVPSQSSTQPDIHEKLAINAIQKSLMQYENVSSPLQPQPQPQFSGSPPAYPMHSMAGGANSSGSGAGSSGGGGGGGGGGGGMSGVGPTGSGPGSSGGTGGPGMNSGSSGMQSSQAGPNQNFTPPPVYTQRMRIPQGQLGGGNNPGVPQNLLAMQKLQHQSRDRILQEQQRTRLLQQQQKQQMVVTVNSAANADLGPPNVTLTRANNVPDSQLSPGFSPSLMQQQLSPSQRTQLSPQQAGFQGNPFNNNPGHRLSPQLQQMVSGFGNAGGNVNQQLSPRQPPFGGGVGGPAVNQPNVVVPGQSPQQQQQQQQQQQQQQQQQQQWQQNANARLSIQQQNPMLNAQLSSVGGFNPAANRQFVGPPQRQRGTLNSPGTPRQQQNAFGGTMVDGGGFPGPPSPSPVGVSAPNFANPVYANQQMRLQRQGSVPPQSTQHLPGSPRSAYGGHGPGPDATGYGMMFGNAAAMQQHAATSPGDFFNRSQTGLNGGHNIGGGGGNSIGGGGGNGGGGGGGGNGPGLSNGTNAPNTGLNHSELVRQELRAIVSGRAHRPPSHSPHMGLSPLGAMSLHGCGPGEGGGSSNGGNLTGSNATSTVGSSNGTCGGSLSNASSAMLGGIGGGGGGGVGGGGGSNGGGGTARTSMADAGTSGAMLHSGGSTAGLDSSMLYNFHLTQKEFFGGNTSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VYK4</accession>
    <name>A0A6E8VYK4_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">CCR4-NOT transcription complex subunit 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the CNOT1 family.</text>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON009057-RA">
        <property type="protein sequence ID" value="ACON009057-PA"/>
        <property type="gene ID" value="ACON009057"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006818"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON009057"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038770"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030015">
        <property type="term" value="C:CCR4-NOT core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000288">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20710">
        <property type="entry name" value="NOT1_connector"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.800:FF:000001">
        <property type="entry name" value="CCR4-NOT transcription complex subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.180:FF:000005">
        <property type="entry name" value="Ccr4-not transcription complex subunit 1 isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.840:FF:000001">
        <property type="entry name" value="Ccr4-not transcription complex subunit 1 isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.790:FF:000004">
        <property type="entry name" value="Not1, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.800">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.840">
        <property type="entry name" value="CCR4-NOT transcription complex subunit 1 TTP binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007196">
        <property type="entry name" value="CCR4-Not_Not1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055454">
        <property type="entry name" value="CNOT1-like_NOT1_connector"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032191">
        <property type="entry name" value="CNOT1_CAF1_bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024557">
        <property type="entry name" value="CNOT1_dom_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032194">
        <property type="entry name" value="CNOT1_HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032193">
        <property type="entry name" value="CNOT1_TTP_bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038535">
        <property type="entry name" value="CNOT1_TTP_bind_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040398">
        <property type="entry name" value="Not1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13162">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13162:SF8">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16415">
        <property type="entry name" value="CNOT1_CAF1_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16418">
        <property type="entry name" value="CNOT1_HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16417">
        <property type="entry name" value="CNOT1_TTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12842">
        <property type="entry name" value="DUF3819"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04054">
        <property type="entry name" value="Not1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23590">
        <property type="entry name" value="NOT1_connector"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1 HEAT repeat" evidence="12">
        <location>
            <begin position="264"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1 TTP binding" evidence="11">
        <location>
            <begin position="592"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1 CAF1-binding" evidence="10">
        <location>
            <begin position="915"/>
            <end position="1138"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1" evidence="9">
        <location>
            <begin position="1259"/>
            <end position="1403"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1-like NOT1 connector" evidence="13">
        <location>
            <begin position="1481"/>
            <end position="1685"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-Not complex component Not1 C-terminal" evidence="8">
        <location>
            <begin position="1865"/>
            <end position="2224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="65"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="445"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="852"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1184"/>
            <end position="1221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="449"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="852"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1196"/>
            <end position="1210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF12842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF16417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF16418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF23590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009057-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2248" mass="247948" checksum="F0AE3634CE19A69F" modified="2020-08-12" version="1">MKTSWTNLVVEVGYSFTATLDDCRNHLLKVGGRDITPQDVAKIVSSMCLTHESLSESSINLPTPSAFWPQGSGDPSGQKGKDGNGGSSASEVTTWKPEVLVQALKEVVPALNWKEVCVALDHPEFLLKDRAGLSLLLTIVKMGMQASNMGQTFPVECLYQRWTNVEGQLSIISLILKNSDLYSFADHIYTSVSLDLLKTPPETDSKEVACWMSLHLVDVLLYIADHANLSEKVMKIFKVPGSLCPDVLFLALLQINPPMTTARQELFTNLVPIFLGNHPNAGTILHHAWNHSTFNVTLRPIILHAMSDWYMVGEGDQSRLSRILDVAQDLKALSNLLNIRTYMFIIDLACLASRREYLKLEKWLADKIRDHGEPFVKTILKFLQRRCPQILGGKYSEEQIPKSSQLPPETLITILNCLQACIASCTPEVADMIHSMMQYGMLLNSKSRAQQQQQQQQAPQQQQQPPPPGVLRSHRGLEAFGANAMGSQLLPPMVDSLNSLTANIGGLSIGGPGGGNSAFSFNNIIATPPSPSRLLPSSSPFPMMPLPPGAAAAAQAGTLSRLQQAPNDKLGAAAAAGAAGIPTQALAPFTADAPLPVSKEVEDEANSYFQRIYNLPPHPTLSIDEVLDMLQRYKDSPNRHENDVYQCMLRNLFEEYKFFPQYPDKELQTTAQLFGGMVERNLVTTYVALGLALRCVLDALKKPEGSKMYYFGITALDRFKNKLHLYPKYCEYVHSIAHFSEFPAHLIEYIEYGSQGQEPPNKTLGPGPLPPSILQFMPSGAAGRGGPVGNPLYRSNSVTGTSNLTLAGVSSSASAAAAAAAAAAAAAAAAGGAGAGTPGKINLSAQLIPPGAAGGGAAGASGGGGAGAGVAGSSGNSGGAGGSSSGGQPPRVKSIANATNIDTLLVATQDREEKIIAPPDAIQDKTAFIFNNLSQINLQQKCEEIKEILQKDYYTWLAQYLVLKRASIEVNFHVLYSNFLDALKIPEINRLVTKETFRNIRVLLRSDKGIANFSDRSLLKNLGHWLGMMTLGRNKPILHLDIDVKSLLVEAYNKGQQELLYVVPFVAKVLESCAKSKVFKPPNPWTMSIMNVLAELHQEPDLKLNLKFEIEVLCKNLNIDVSDLKPAIYLKDPERAQNIEYQLSQPKPPKETPAAAVMGGAGVAGLGVGGNVGGGGGGGGGGGVVAVVPQLPPGAGPDDMGSSGPSSSPANVAMDPALAATGPPEPRFHYSDINIANPSLNAQHIVYHPTVAILHTQTHLKVIVKSCLERTISEWISLIVDRSVKIASKTTEQIVRKDFALDSDETRMRRSAHNMARSLVAGMAMITCRDQMVQTIQNNIKVAFAAAVGSAQKEAIIEHAAQQLAMDNIELVTAFIQKTAIEKVAIEMDKILAEDFQLRKLARQEGRRYWDAAVLSYHAERMPERIKLNVGGVSPSQLAVYEEFSRNIPGFLPITERDAAQFAPKMAEMLPFVATPDEIGAIYDELASKMDTFIKTTRLVPILQLHTNNMEQLLECLIHAHRSLDNLTGCTLLNKAVEGLLEGLINIPDQIEHVKLYRDIHLRVMRLMQDHRLFGPMWTNKAITRYMLECREELRYNVEAVDLLITSNFVNMQQFDMMLVQLMDNGNNYVAVVFAMQLLQTFFIDERHNSAITENDLAGTIEMLHRLTAHPRAPEGLTHLIEMLRANHDPNSFLMDRAIVGPTSYIHAGVAQARSDIDDSPGFLERAEFLLKDWVTIALSPNTCRDPLKGFSVFVGKMNAHGILKGDEPLTRFFRFATQYCIDLTYRNMNEPNAKTKIFQFIDAYVRLIALLVKHSGESGSTNTKLNLLNKILGIIIGILLHDQEVHTTAFQQVGYHRIFAMLFLELTTHDPILENISISVITAFCHTFHILRPSAAPGFCYSWLELIAHRVFIGRVLAQIPQQKGWHMYSQLLIDLFKYLAPFLRNAELAKPVQHLYKGTLRVLLVLLHDFPEFLCDYHFAFCDVIPSNCIQMRNLILSPYPRNMRLPDPFTPNLKVDMLNDIGGSPRISINYASSIQPVSFKKDLDSYLKARAPVTFLSELRSNLQISNEVGSRYNIPLMNALVLYVGTQAIAHIRSKNLGPTMSTIIHSAHMDIFQNLAVDLDTEGRYLFLNAIANQLRYPNSHTHYFSCCILYLFAEANSEAIQEQITRVLLERLIVNRPHPWGLLITFIELIKNPIYKFWDHDFVHCAPEIERLFESVAKSCMVTSKSQQQIQNVEPDITECS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VYK7</accession>
    <name>A0A6E8VYK7_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Anion exchange protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the anion exchanger (TC 2.A.31) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VYK7"/>
    <dbReference type="EnsemblMetazoa" id="ACON009736-RB">
        <property type="protein sequence ID" value="ACON009736-PB"/>
        <property type="gene ID" value="ACON009736"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012202"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON009736"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032724"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008509">
        <property type="term" value="F:monoatomic anion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008510">
        <property type="term" value="F:sodium:bicarbonate symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005452">
        <property type="term" value="F:solute:inorganic anion antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051453">
        <property type="term" value="P:regulation of intracellular pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.570:FF:000001">
        <property type="entry name" value="Anion exchange protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.570">
        <property type="entry name" value="Helical hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.930.10">
        <property type="entry name" value="Mannitol-specific EII, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013769">
        <property type="entry name" value="Band3_cytoplasmic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011531">
        <property type="entry name" value="HCO3_transpt-like_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003020">
        <property type="entry name" value="HCO3_transpt_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016152">
        <property type="entry name" value="PTrfase/Anion_transptr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00834">
        <property type="entry name" value="ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11453">
        <property type="entry name" value="ANION EXCHANGE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11453:SF36">
        <property type="entry name" value="ANION EXCHANGE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07565">
        <property type="entry name" value="Band_3_cyto"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00955">
        <property type="entry name" value="HCO3_cotransp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01231">
        <property type="entry name" value="HCO3TRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55804">
        <property type="entry name" value="Phoshotransferase/anion transport protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="525"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="554"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="610"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="724"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="762"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="811"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="856"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="911"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="937"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="domain" description="Band 3 cytoplasmic" evidence="12">
        <location>
            <begin position="105"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="domain" description="Bicarbonate transporter-like transmembrane" evidence="11">
        <location>
            <begin position="496"/>
            <end position="1041"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="42"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="234"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="302"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="451"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1111"/>
            <end position="1186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="46"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="235"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="245"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="304"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="462"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1111"/>
            <end position="1123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1124"/>
            <end position="1134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1163"/>
            <end position="1186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF07565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009736-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1212" mass="134985" checksum="DE0A1F8E085317B9" modified="2020-08-12" version="1">MMDHGVVDEEAPIDPRLKNRTFTADQDFEGHRAHTVFVGVHIPGSSRRHSQRRRHKHHQASRENGDKGSTGSEAERPVTPPAQRVQFILGGEVGGEGDGTQHESHPLFSEMEELVKEGDEMAWKETARWVKFEEDVEEGGNRWSKPHVATLSLHSLFELRSLLLNGTVMLDMEAVSLEQIAELVCENMVNSGTLPVEARDKVIDALLKRHKHQHEFGSKKSRLPLIRSLADIGKNHSSSKNMPRSSTSKSISSANSFPMHVVSSAPSGMHEEGESALGPVSPQTALLYGSKDQRGHYLALPTGENMTQSPSNVSMPRNASSGELQNGEHKTNTHFMRKIPPGAEASNILVGEVDFLDKTLSAFLRLNTASVMGDLTEVPVPTRFIFILLGPPGSHGSFHEIGRAMATLMSDEIFHEVAYRAKRREHLLAGIDEFLDAVTVLPPGEWDPSIRIEPPAAIPSQEVRKRPPEKNPKEEIDEELEEQRQREEAGLSRTGRLFGGLINDLKRKAPFYLSDFKDGLSMQCVASWIFLYFACLSPIITFGGLLGTATGNNIAAMESLVSGFVCGIGYGFFSGQPLTILGSTGPVLVFETIVYEFCQKVGWDYLTFRFWIGTWISIILVVLVAVDASALVCYITRFTEENFACLIAVIFIYKAIENVFHIGQEYPLNTAGGKYDCACLPPAAHELTPEAIHQWAQYDLRTCKTLNGTLTGADCDKPEYVSDVFLMSIVLFLGTYIISVILKDFKNALFFPAVVRQFISDFSVTIAIFSMTLLDVFTRIATPKLDVPSEFKRTIPDRGWIIMPFHESNPAWSSALAILPALLGTILIFMDQQITAVIINRKEHKLTKGCGYHLDLFVLACLIQICTMMGLPWFVAATVLSINHVNSLKKESETAAPGEKPQFIGVREQRVTHILIFLMIGCSVLLTPLLSHIPMPVLYGVFLYMGVSALKGLQFFDRLLIMLMPAKYQPDYMFLRQVPIRRVHLFTMIQLACFAVLWLIKSFSITSILFPLMLVVMIGVRKSLDYIFTKRELKILDDIMPEMTKRARADDLHQLEDGEDSGQHSADNLQLPLENGTVNEKGKINISEEVNRTTIWKQVNNCNVLFTKKQTPVKHSQQQLSKLSETEKRLSTMREEDEVADEPSRQLSHQKRTQKMKREFWMNSRSGSSNGRANSNNSASSTTAAAGGKIVCAEESAALSRGSSKSASETQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W073</accession>
    <name>A0A6E8W073_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Choline transporter-like protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Choline transporter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the CTL (choline transporter-like) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040233136.2">
        <property type="nucleotide sequence ID" value="XM_040377202.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8W073"/>
    <dbReference type="EnsemblMetazoa" id="ACON010343-RA">
        <property type="protein sequence ID" value="ACON010343-PA"/>
        <property type="gene ID" value="ACON010343"/>
    </dbReference>
    <dbReference type="GeneID" id="120955942"/>
    <dbReference type="CTD" id="110281"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006881"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON010343"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042413"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007603">
        <property type="entry name" value="Choline_transptr-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12385">
        <property type="entry name" value="CHOLINE TRANSPORTER-LIKE (SLC FAMILY 44)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12385:SF14">
        <property type="entry name" value="CHOLINE TRANSPORTER-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04515">
        <property type="entry name" value="Choline_transpo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="48"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="233"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="261"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="316"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="370"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="465"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="509"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="611"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="644"/>
            <end position="669"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU368066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010343-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="707" mass="79902" checksum="9F416F1B5BA3EEA2" modified="2020-08-12" version="1">MGLCCGSTDRVTKYSKEDIQQPTESEPLKYDPDFKGPLSKRSCTDLPCLFLFVTFLCAWGYVAYYAVQHGDLNRLLVPIDSDGRKCGVDSEVRDEPYLVFFNITECAKIDVPISGCSTTQVCVSQCPNQDFDFESANCNPSNVNEIRSKLICKQNVKKSDLTTCQIIRQYIKSQRCAQTMQKSTPFSKRCIPDMGTITNGARNVQRSIDVAIKNLKLFIGTSQMIVEDILESWRMILVFIACSVLASLILIAMLRWIAKPLVWISIIGVITALSYGVYYSFRQYQQIRANPVAAHVNVSPNLSSLVNSWFKSDQTWLWILIALSVILIVLLLVVLVLRKRIVIAIALLKEGSKAVSASFSTVFFPLVPWILQAVVIVFSLLVLLFLASIGVPVYKVNGLNSSLTCVCTNGYMEGDICDPVAFNENCRDTSRIYDQERCLDAACHFQEVDTPGIVRFFHALNVIGFFWCICFVSAFSEMVLAFTFATWYWTRQKSRLPFFVLTRGVTHTVYYHLGTLAFGSLIIAICKIIRAILEYVDHKLKRYDNGFTRAVLCCCRCFFWCLESFLKFLNRNAYIMCAIYGKNFCSSAKDAFSLLTRNVLRVIALDKVTGFLFFLSKLLLASGMAAVTYTYFDSDLPKMQLNYPFVPAVLVFIGTFIIASIFFSVYSVAVDTLFLCFLEDIERNDGSAERPFYMSRGLQKILGKKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W1L9</accession>
    <name>A0A6E8W1L9_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BAG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_040232972.1">
        <property type="nucleotide sequence ID" value="XM_040377038.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8W1L9"/>
    <dbReference type="EnsemblMetazoa" id="ACON010983-RA">
        <property type="protein sequence ID" value="ACON010983-PA"/>
        <property type="gene ID" value="ACON010983"/>
    </dbReference>
    <dbReference type="GeneID" id="120955847"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000328"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON010983"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037834"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000774">
        <property type="term" value="F:adenyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037689">
        <property type="entry name" value="BAG2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12334">
        <property type="entry name" value="BAG FAMILY MOLECULAR CHAPERONE REGULATOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12334:SF6">
        <property type="entry name" value="BAG FAMILY MOLECULAR CHAPERONE REGULATOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010983-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="207" mass="23412" checksum="EE118A70876D6C65" modified="2020-08-12" version="1">MGDEWSRARGGGGRCAKRSRFDQQFDAEFGPSAVTSTTRFIDILDQLDTKVESLRKEAMVLRDKKDFLAMSVDLLKNNEYLSGLNENEREEIDCYVQRISSRLGTVELNVCTVRDQAQEDSLHHVNSLIDLIIASADPVISRQKCQQYLNACSTTDTSVYTDVDPHTVCTDKKFESVLLGCTLDDQKTIKKRLQALLVYLTQQTIVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W1M1</accession>
    <name>A0A6E8W1M1_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="11">FIP-RBD domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
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        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
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                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
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                <person name="Korlach J."/>
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            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain>Mali-NIH</strain>
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    <reference evidence="11" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
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            <strain evidence="11">Ngousso</strain>
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        <location>
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            <end position="504"/>
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            <dbReference type="PROSITE" id="PS51511"/>
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        <source>
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        </source>
    </evidence>
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</entry>
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            <taxon>Nematocera</taxon>
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            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
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        <subcellularLocation>
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        </subcellularLocation>
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    <dbReference type="AlphaFoldDB" id="A0A6E8W1M6"/>
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        <property type="gene ID" value="ACON010143"/>
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    <dbReference type="Proteomes" id="UP001105220">
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    </evidence>
    <sequence length="448" mass="49077" checksum="0E366F718D203DBF" modified="2020-08-12" version="1">MGRKKKKASKPWCWYCNREFDDEKILVQHQKAKHFKCHICHKKLYTGPGLSIHCMQVHKESIDKVPNSLPNRSNIVIEIYGMEGIPPEDIREHEKQKNGNRSESEEEEPAHKKMKQAEASVSQGMMQMQNMMPPHLQAAYSINPMMSAMGGINPYMVPPGMPMMPAMMAGAQRPLFPAAAAASAATVTSASKPTFPAYSSATISAPPTTNSAISAAASGNSNNGLGGGDATKGASSSIMPNSGTATSKIVHPPEDLSLEELRARKPQYQKQIQQATQQMLQQKQQEKQQAQVAAAVAAAQQQLQAQAQAQLKQHHQQQQQQHQQQLHQQQQQHQQQQQQQQQQQQQQQHQQQQQLQQLSPFSSAAGTASIVSNTQTTAAMKDNRMMSPQEVPVSLGGPVPLMSPMMRQGLAIGPPGGGNLALLGGNMLRPGPPQMGLGPGRYNQYYRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W1X2</accession>
    <name>A0A6E8W1X2_ANOCL</name>
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        <recommendedName>
            <fullName evidence="2">NAD(+) kinase</fullName>
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        </recommendedName>
    </protein>
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        <name type="scientific">Anopheles coluzzii</name>
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            <taxon>Diptera</taxon>
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            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
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    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
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                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAD kinase family.</text>
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        <property type="gene ID" value="ACON011122"/>
    </dbReference>
    <dbReference type="GeneID" id="120955473"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_013407"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON011122"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038828"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003951">
        <property type="term" value="F:NAD+ kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019674">
        <property type="term" value="P:NAD+ metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006741">
        <property type="term" value="P:NADP+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.30:FF:000003">
        <property type="entry name" value="NAD kinase b"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NAD kinase isoform X1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
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        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 2"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="ATP-NAD_kinase_N"/>
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        <property type="entry name" value="ATP-NAD_kinase_PpnK-typ_C"/>
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    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
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        <property type="entry name" value="NADK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20275">
        <property type="entry name" value="NAD KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20275:SF0">
        <property type="entry name" value="NAD KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01513">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20143">
        <property type="entry name" value="NAD_kinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
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        <location>
            <begin position="22"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="22"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="38"/>
            <end position="50"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012120"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
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    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON011122-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="478" mass="53471" checksum="D6D3C6EF055ED166" modified="2020-08-12" version="1">MAEMHENDDFMADLVRFNHHPSAHHHHHQNHHNHLHHMFGSSGHQQQQQNNHRHHQSNGAGGGYSPSAEDDIPADEDQLLLWRPKRRTRSLNAPSPFQQFGPCGRIMKNSAMVMQIQDPASQRLTWYKPPLAVLVIKKVRDSKVLQPFVQLVEWLIHEKHMVVWVEAAILDDALLTGDKRFTKLQDKLITFKDGRDDLTDKIDFIICLGGDGTLLYASLLFQKSVPPVMAFHLGSLGFLTPFQFDNFQEQVTNVLEGHAALTLRSRLRCIIVRKDKTEQEISTFKSSQDPSTNILVLNEVVIDRGLSSYLSNIDLFLDGKHITSVQGDGLIVSTPTGSTAYSAAAGASMIHPSVPAILVTPICPHSLSFRPIVLPAGVELKIAISPDSRNSSWVSFDGRNRQELLHGDSLHVTTSIYPVPSICAQDQIADWFDSLAECLHWNVRKRQKCLDELSDLTGSGTEDSAIDDIVRGIENLEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W413</accession>
    <name>A0A6E8W413_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Structural maintenance of chromosomes protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8W413"/>
    <dbReference type="EnsemblMetazoa" id="ACON011425-RB">
        <property type="protein sequence ID" value="ACON011425-PB"/>
        <property type="gene ID" value="ACON011425"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011445"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON011425"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040210"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000385">
        <property type="entry name" value="Structural maintenance of chromosomes 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003395">
        <property type="entry name" value="RecF/RecN/SMC_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43977">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02463">
        <property type="entry name" value="SMC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RecF/RecN/SMC N-terminal" evidence="3">
        <location>
            <begin position="118"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="27"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="206"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="231"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON011425-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="436" mass="50033" checksum="D4B2AEF0B307E198" modified="2020-08-12" version="1">TLQKTIVEARETQTQCSAKVKDLQAKIADGKGHRERELKSAEEDLKRSKKKSEESRKNWKKHEQDFETLKLEIEELQKGIVTAKEQAVKLEEQITALQQRLVEVSGTTDEMTAAVTALKQQIKQHKEKMNSQSKELKAKYHQRDKLLKQNDELKLEIKKKENEITKVRNENKDGYDRISGMEQKYPWIPEDKEFFGVKNTRYDYNKEDPQEAGRKLKKLQDSKDKMSRNVNQKAMVLLEREEEQYKEVMRRKKVVEDDKKKIQAIITDLDEEKKKKLKVAWSEVDENFGSIFSTLLPGTQARLVPPDGVDFMKGLEVKVGFNGMWKESLTELSGGQRSLVALSLILAMLKYKPAPLYILDEVDAALDLSHTQNIGNMLKAHFTNSQFIIVSLKDGMFNNANVLFRTKFIDGMSGVTRTVNVANIKRAAADAASRGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W416</accession>
    <name>A0A6E8W416_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Chitin-binding type-2 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8W416"/>
    <dbReference type="EnsemblMetazoa" id="ACON011033-RB">
        <property type="protein sequence ID" value="ACON011033-PB"/>
        <property type="gene ID" value="ACON011033"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007060"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON011033"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_036113"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004568">
        <property type="term" value="F:chitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02872">
        <property type="entry name" value="GH18_chitolectin_chitotriosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000179">
        <property type="entry name" value="Chitinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.50.10:FF:000001">
        <property type="entry name" value="Chitinase 3-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.140.10">
        <property type="entry name" value="Chitin binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002557">
        <property type="entry name" value="Chitin-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036508">
        <property type="entry name" value="Chitin-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011583">
        <property type="entry name" value="Chitinase_II/V-like_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029070">
        <property type="entry name" value="Chitinase_insertion_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001579">
        <property type="entry name" value="Glyco_hydro_18_chit_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050314">
        <property type="entry name" value="Glycosyl_Hydrlase_18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11177">
        <property type="entry name" value="CHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11177:SF360">
        <property type="entry name" value="CHITINASE 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01607">
        <property type="entry name" value="CBM_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00494">
        <property type="entry name" value="ChtBD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00636">
        <property type="entry name" value="Glyco_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54556">
        <property type="entry name" value="Chitinase insertion domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57625">
        <property type="entry name" value="Invertebrate chitin-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50940">
        <property type="entry name" value="CHIT_BIND_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01095">
        <property type="entry name" value="GH18_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0147">Chitin-binding</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026295938" evidence="8">
        <location>
            <begin position="20"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="domain" description="GH18" evidence="10">
        <location>
            <begin position="22"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="9">
        <location>
            <begin position="441"/>
            <end position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON011033-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="499" mass="54625" checksum="E0BE329C294EC5D2" modified="2020-08-12" version="1">MKVAAVAGLLLISVASINAATDRIVCYFGSWATYRIGNGKYDVENINPNLCTHIVYTFVGLDTKGNVKILDNWLDISLGGYSRFIQLKQRNPKVKLMVAIGGWNEGSASYSTMANSDLLRAVFVESAVAFVKRYGFDGFDVDWEYPTLRGGSVDDRVGFVKLLRDLRARFDQEGLLLSIATAATADYLRSAYDVPEINKYVHFVNLMSYDLHAYWDAQTGANAPMYPNSWETGYTSSMLNVDACVKAWLGAGLEPSKLVLGVPVFGHTFKLSSTSDTRIGAPTIGPGDAGPYTLEPGTLSYLEICEKLAAGDYTKAFSSVQQVPYAYRGNQWISYDDVNSIAIKVQYAKRMNLGGIMVWSIESDDARGICGEGQHPITSAVYREVFGTGAPTPGTTTTTAKTTTTSTARPITTTTTQRPLTTTTTTYRTTTTATTARPNQKLVCPASGFMRDPNNCAQFYQCYPGLPIQENWLLKCPSGLFFDPKASVCTYLFNTDCTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W427</accession>
    <name>A0A6E8W427_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Protein SERAC1</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8W427"/>
    <dbReference type="EnsemblMetazoa" id="ACON011044-RB">
        <property type="protein sequence ID" value="ACON011044-PB"/>
        <property type="gene ID" value="ACON011044"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000290"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON011044"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038579"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48187">
        <property type="entry name" value="LD21810P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48187:SF2">
        <property type="entry name" value="LD21810P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON011044-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="388" mass="44675" checksum="A751CC2094E4F542" modified="2020-08-12" version="1">MQIEYRKCTKLFSGCGVASLLGVLSYEAYRTYAALSSLVDTRVLDIDRKRPDYIYIKHHIYRQSLRERALDAENQHRLLNIIVQPFEKWWKALKHSVAWRLLRIAQRGDQAERLKAIHQLAQIDHLKDWDFQHLAQLCDYRTAISLARSNCDTRWFLPPRQYGVEKEMREVLVEMKSLLELLAPHKCAAYALEQTFGSSGHIREPDGDEVTYQMSPSRFEYDQLKQSLSALVHLTGLVGPEGKQHCRRIVDAGGLLMLLEAEKLFREDIDMKLLISRLVGNLSACEGLGYEFYASGWIGILARWKRDVDMRMQVTADLTLENLDQDDPNGFRYAPKVYPLHPRGRQQAKPAVDVVFIHGLLGGVFVTWRQKDLKPQAASLLGHYRVQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W445</accession>
    <name>A0A6E8W445_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ANK_REP_REGION domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="EnsemblMetazoa" id="ACON012141-RA">
        <property type="protein sequence ID" value="ACON012141-PA"/>
        <property type="gene ID" value="ACON012141"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006444"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON012141"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038750"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030165">
        <property type="term" value="F:PDZ domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019887">
        <property type="term" value="F:protein kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011646">
        <property type="entry name" value="KAP_P-loop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052771">
        <property type="entry name" value="Neurotrophin_sig_adaptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057092">
        <property type="entry name" value="SAM_KIDINS220"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24116">
        <property type="entry name" value="KINASE D-INTERACTING SUBSTRATE OF 220 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24116:SF0">
        <property type="entry name" value="KINASE D-INTERACTING SUBSTRATE OF 220 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07693">
        <property type="entry name" value="KAP_NTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23307">
        <property type="entry name" value="SAM_KIDINS220"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="501"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="526"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="660"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="692"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="38"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="71"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="104"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="171"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="204"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="237"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="270"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="303"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="336"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="domain" description="KAP NTPase" evidence="4">
        <location>
            <begin position="444"/>
            <end position="972"/>
        </location>
    </feature>
    <feature type="domain" description="Kinase D-interacting substrate of 220 kDa-like SAM" evidence="5">
        <location>
            <begin position="1235"/>
            <end position="1311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="869"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1178"/>
            <end position="1227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1336"/>
            <end position="1357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1438"/>
            <end position="1489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1507"/>
            <end position="1530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="869"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1199"/>
            <end position="1225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1343"/>
            <end position="1356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="1449"/>
            <end position="1467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1468"/>
            <end position="1478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON012141-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1530" mass="166513" checksum="5BBD1667BF83F74E" modified="2020-08-12" version="1">MGSLGHRSLLQYLETDDLAGLKSFLGTRHLQVDDRDENNTTVLMVASGRGATHFVKELLARGADVQAQDLDSWTALHFAAKAGHVGIVELLLDNGAELEHRDMGGWTALMWGSYKGHTSVVALLLQRGADVQAHGNYHLNPLLWASGRGHTEIVRLLVNTGGAKVNVGDKYGTTPLVWACRKGSAEIVDVLLKAGANVDTAGMYSWTPLLVAVSGGFQECVSLLLERKPNVNALDKDGMTALSIACREGLTEIASALIAAGAYLNVQDRAGDTPLINAVKGGHRSVVEILMKRHVDVDIQGKDKKTALYTAVEKGHTAIVKLILQSNPDLELSTKDGDTALLRAVRNRNLEIVQMLLERKAKVGATDKRGDTCLHVAMRARSKAIVEALLSNPKYGQLLYRSNKEGETPYAIDATHQKTILGQVFGNRRLNANEDSEGMLGYGLYSSALADVLSEPTLTTPITVGLYAKWGSGKSFLLTELRDEMKNFAHSWSEPPIDASWLFFLISLHLVLVIGTVVGLATWSYVWGMVTGATLLVLIYFTDILLKFLDRRYDLEWLYSLNYGLSRKLGRLRLILQVAFCHPPGPQSDQQPMPVRFHFAEASGAAPNGDAAVALMLASLFDAIEAHYGSLATGLYRAFRPKPLKATGGWKWRKMCCMPVVLMFELGMLGILATASLSIVYSEYGLEGREEIAVAIYVLLGILLAGAIANLHAWSKLIGALFMSQGKHLKRAFNSNEAAPLTALGAEVSLMTDMVRCLDAFTGQQSRLVGVVDALDSCDTERTLTILNAVQTLLSGPQRPFVLLLAVDPHVIAKAAEANSRRLFTEGGIGGHDFLRNLVHLPVYLQNSGLRKVQRAQNTAMSAYRRAMPDLSRDDDQPHLGHSASARRLSNASEIMSSQEKLRAMPSSSRGGSKKLRVSDSIASSIGSNLHKLGQNPPVDLSKIILTDDYFSDVNPRSMRRLMNVIYITVRLLKAFQIDFSWYRLSSWINLTEQWPLRASMIVLEHDQGGDSFDDSVSLQAVYDKVRPKLTCLREAAALLDLDRDERKLDAFLQLHKSDLLVSDLRIFLPFTINLDPYLRKVLKEDQQALEDEGIIIPMKTVLPPSKPSGFVPHHHHRAPVGGVLQPTPQHPNNLTNWPNFYNPQPQAMALMSYYNQNWANFPAGVYGTNALLGEPLPKPGSVGGGPIITEHPPHEQHSTLGSNSGRSSKTTTGALQKSSSNGLATSPPIDIDLSNVQLSSLTVEQLIELLEKVNDLKPAMERTAPILRENAISGRVLMYCNLEELKSVLRLSFGHWEMFKLLVTALREASVTQPASRKLSTKTTSFAKGTNDSVEMQEPIAQSTPPFGSSTSSFQPIRQKSQNLLEKQVTLEEQMICGALQTLNEDAFEDVVSSSERPSPTGEMFSQYLAPIRESSEIGSPPRLTYNLTNPNLTDLAASNGTLNGDDSGVGGGGSVGVGGGGGGSTSSGSHLGLRSGSGRHSRSHSLHDDASLTSIVVIPTFLTTSPNGASSSNNNNNNTTTNINDTKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W6A2</accession>
    <name>A0A6E8W6A2_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="7">RRM domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8W6A2"/>
    <dbReference type="EnsemblMetazoa" id="ACON013145-RA">
        <property type="protein sequence ID" value="ACON013145-PA"/>
        <property type="gene ID" value="ACON013145"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010950"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON013145"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031655"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12684">
        <property type="entry name" value="RRM_cpo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000037">
        <property type="entry name" value="RNA-binding protein with multiple splicing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034788">
        <property type="entry name" value="Cpo_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10501">
        <property type="entry name" value="U1 SMALL NUCLEAR RIBONUCLEOPROTEIN A/U2 SMALL NUCLEAR RIBONUCLEOPROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="248"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="84"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="93"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON013145-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="411" mass="42916" checksum="2603826B79728E8C" modified="2020-08-12" version="1">SAHGVRFSGASVNSVTISSTVVKRRPAPQITVASGPQVLSPKRLRTTALPGVAAAALPALGNCLVPAHPSAAAAAAAAAALLQHQIHQHHHQQQQQQQQQQQQHLQQLHHQQSIGSDAQVQQVQQSISPQPNQVSVSVASALTSQSPLGTIPVSSTGTGAVGAVGQPPMFSSPVVAQSNTVVSSPSMNGALNVNAANNNHTGLGQTGQSATGNLKMDSSMGQMTPMAPLALSQSMDSVNTASNEEEVRTLFVSGLPMDAKPRELYLLFRAYEGYEGSLLKVTSKNGKTASPVGFVTFNTRAGAEAAKQDLQQGVRFDPDMPQTIRLEFAKSNTKVSKPKPQPNTAATAAHPALMHPLTAGRTPFFPGGPELWHHPLAFSAAAAELPSAAALQHATLVHPALHPQVPVRSYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W6A3</accession>
    <name>A0A6E8W6A3_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Alanine--glyoxylate aminotransferase 2, mitochondrial</fullName>
            <ecNumber evidence="28">2.6.1.18</ecNumber>
            <ecNumber evidence="12">2.6.1.40</ecNumber>
            <ecNumber evidence="5">2.6.1.44</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">(R)-3-amino-2-methylpropionate--pyruvate transaminase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Beta-ALAAT II</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Beta-alanine-pyruvate aminotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="30">D-3-aminoisobutyrate-pyruvate aminotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">D-AIBAT</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="29">D-beta-aminoisobutyrate-pyruvate aminotransferase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="41 42">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="41" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="41">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="38">Multifunctional aminotransferase with a broad substrate specificity. Catalyzes the conversion of glyoxylate to glycine using alanine as the amino donor. Catalyzes metabolism of not L- but the D-isomer of D-beta-aminoisobutyric acid to generate 2-methyl-3-oxopropanoate and alanine. Catalyzes the transfer of the amino group from beta-alanine to pyruvate to yield L-alanine and 3-oxopropanoate. Can metabolize NG-monomethyl-L-arginine (NMMA), asymmetric NG,NG-dimethyl-L-arginine (ADMA) and symmetric NG,N'G-dimethyl-L-arginine (SDMA). ADMA is a potent inhibitor of nitric-oxide (NO) synthase, and this activity provides mechanism through which the kidney regulates blood pressure.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>(2S)-2-aminobutanoate + glyoxylate = 2-oxobutanoate + glycine</text>
            <dbReference type="Rhea" id="RHEA:77339"/>
            <dbReference type="ChEBI" id="CHEBI:16763"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:74359"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>(R)-3-amino-2-methylpropanoate + pyruvate = 2-methyl-3-oxopropanoate + L-alanine</text>
            <dbReference type="Rhea" id="RHEA:18393"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:57700"/>
            <dbReference type="ChEBI" id="CHEBI:57731"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="EC" id="2.6.1.40"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:18394"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>2-oxobutanoate + L-alanine = (2S)-2-aminobutanoate + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:77355"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:16763"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:74359"/>
            <dbReference type="EC" id="2.6.1.44"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="33">
            <text>2-oxohexanoate + N(omega),N(omega)-dimethyl-L-arginine = L-2-aminohexanoate + 5-(3,3-dimethylguanidino)-2-oxopentanoate</text>
            <dbReference type="Rhea" id="RHEA:77363"/>
            <dbReference type="ChEBI" id="CHEBI:35177"/>
            <dbReference type="ChEBI" id="CHEBI:58326"/>
            <dbReference type="ChEBI" id="CHEBI:58455"/>
            <dbReference type="ChEBI" id="CHEBI:197301"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>2-oxopentanoate + N(omega),N(omega)-dimethyl-L-arginine = 5-(3,3-dimethylguanidino)-2-oxopentanoate + L-2-aminopentanoate</text>
            <dbReference type="Rhea" id="RHEA:77359"/>
            <dbReference type="ChEBI" id="CHEBI:28644"/>
            <dbReference type="ChEBI" id="CHEBI:58326"/>
            <dbReference type="ChEBI" id="CHEBI:58441"/>
            <dbReference type="ChEBI" id="CHEBI:197301"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>3-oxopropanoate + L-alanine = beta-alanine + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:14077"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:33190"/>
            <dbReference type="ChEBI" id="CHEBI:57966"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="EC" id="2.6.1.18"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="26">
            <dbReference type="Rhea" id="RHEA:14079"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="32">
            <text>L-ornithine + glyoxylate = 5-amino-2-oxopentanoate + glycine</text>
            <dbReference type="Rhea" id="RHEA:77331"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:58802"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>L-ornithine + pyruvate = 5-amino-2-oxopentanoate + L-alanine</text>
            <dbReference type="Rhea" id="RHEA:77327"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:58802"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="37">
            <text>N(omega),N('omega)-dimethyl-L-arginine + glyoxylate = 5-(3,3'-dimethylguanidino)-2-oxopentanoate + glycine</text>
            <dbReference type="Rhea" id="RHEA:77315"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:197308"/>
            <dbReference type="ChEBI" id="CHEBI:197310"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>N(omega),N('omega)-dimethyl-L-arginine + pyruvate = 5-(3,3'-dimethylguanidino)-2-oxopentanoate + L-alanine</text>
            <dbReference type="Rhea" id="RHEA:77307"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:197308"/>
            <dbReference type="ChEBI" id="CHEBI:197310"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="34">
            <text>N(omega),N(omega)-dimethyl-L-arginine + 2-oxobutanoate = 5-(3,3-dimethylguanidino)-2-oxopentanoate + (2S)-2-aminobutanoate</text>
            <dbReference type="Rhea" id="RHEA:77351"/>
            <dbReference type="ChEBI" id="CHEBI:16763"/>
            <dbReference type="ChEBI" id="CHEBI:58326"/>
            <dbReference type="ChEBI" id="CHEBI:74359"/>
            <dbReference type="ChEBI" id="CHEBI:197301"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="31">
            <text>N(omega),N(omega)-dimethyl-L-arginine + glyoxylate = 5-(3,3-dimethylguanidino)-2-oxopentanoate + glycine</text>
            <dbReference type="Rhea" id="RHEA:77311"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:58326"/>
            <dbReference type="ChEBI" id="CHEBI:197301"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>N(omega),N(omega)-dimethyl-L-arginine + oxaloacetate = 5-(3,3-dimethylguanidino)-2-oxopentanoate + L-aspartate</text>
            <dbReference type="Rhea" id="RHEA:77343"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:58326"/>
            <dbReference type="ChEBI" id="CHEBI:197301"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>N(omega),N(omega)-dimethyl-L-arginine + pyruvate = 5-(3,3-dimethylguanidino)-2-oxopentanoate + L-alanine</text>
            <dbReference type="Rhea" id="RHEA:77303"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:58326"/>
            <dbReference type="ChEBI" id="CHEBI:197301"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="35">
            <text>N(omega)-methyl-L-arginine + glyoxylate = 5-(3-methylguanidino)-2-oxopentanoate + glycine</text>
            <dbReference type="Rhea" id="RHEA:77323"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:114953"/>
            <dbReference type="ChEBI" id="CHEBI:197314"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>N(omega)-methyl-L-arginine + pyruvate = 5-(3-methylguanidino)-2-oxopentanoate + L-alanine</text>
            <dbReference type="Rhea" id="RHEA:77319"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:114953"/>
            <dbReference type="ChEBI" id="CHEBI:197314"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>glyoxylate + L-alanine = glycine + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:24248"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="EC" id="2.6.1.44"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:24249"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="36">
            <text>oxaloacetate + L-alanine = L-aspartate + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:77347"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 39">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.18" evidence="28"/>
    <dbReference type="EC" id="2.6.1.40" evidence="12"/>
    <dbReference type="EC" id="2.6.1.44" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8W6A3"/>
    <dbReference type="EnsemblMetazoa" id="ACON012404-RB">
        <property type="protein sequence ID" value="ACON012404-PB"/>
        <property type="gene ID" value="ACON012404"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_013487"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON012404"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033800"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047305">
        <property type="term" value="F:(R)-3-amino-2-methylpropionate-pyruvate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008453">
        <property type="term" value="F:alanine-glyoxylate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016223">
        <property type="term" value="F:beta-alanine:pyruvate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009436">
        <property type="term" value="P:glyoxylate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019481">
        <property type="term" value="P:L-alanine catabolic process, by transamination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000055">
        <property type="entry name" value="Alanine--glyoxylate aminotransferase 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049704">
        <property type="entry name" value="Aminotrans_3_PPA_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8 39" id="KW-0663">Pyridoxal phosphate</keyword>
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        <location>
            <begin position="24"/>
            <end position="54"/>
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        <source>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="42">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
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    </evidence>
    <sequence length="506" mass="56344" checksum="E7330DDE136762AF" modified="2020-08-12" version="1">MLLMNLQRLSASSLGGRSLCRRLTTQPNTQHPESLKRQQRQTQTQHESPPYKGQSFDEVVELRKQYLTPNVTSYYKKPLLIHKGSGSQLFDQDKSYLDMFGGIVTVSVGHCHPKVNAALEEQLRTLWHTTNIYMHPKLHQYAEKLVAKMPGDLKNVYFVNSGSEANDLAMMIARLYTGNHDIISFRNAYHGASPYTMGLTAHSTWRYPLPGLNSGILHAMNPDPYTGIWGGKQCRDSPVQTTRSCDCQEGQCKATDMYYDQLEQLFKYSLPRGKVAGMFAESIQGVGGTVQYTKGYIKRAAELVRANGGLFISDEVQSGFGRTGEHYWGFEAHDIVPDIVTMAKGIGNGFPMGAVVTSRKVAEVLCQALHFNTFGGNPLACAVGMAVLDVIDEEELQKNSLDVGTYMLKGLERLRDKHDVIGDVRGKGLMIGVELVADKETRQHLSAPHFVDIWEMCKDMGVLFGRGGLNANVLRIKPPMCITKADVDFSLQVLDIALRKHSEKTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W6D1</accession>
    <name>A0A6E8W6D1_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Voltage-dependent L-type calcium channel subunit alpha</fullName>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 16">Membrane</location>
            <topology evidence="1 16">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14 16">Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8W6D1"/>
    <dbReference type="EnsemblMetazoa" id="ACON012434-RA">
        <property type="protein sequence ID" value="ACON012434-PA"/>
        <property type="gene ID" value="ACON012434"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004210"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON012434"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041833"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005891">
        <property type="term" value="C:voltage-gated calcium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008331">
        <property type="term" value="F:high voltage-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098703">
        <property type="term" value="P:calcium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009582">
        <property type="term" value="P:detection of abiotic stimulus"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009581">
        <property type="term" value="P:detection of external stimulus"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050906">
        <property type="term" value="P:detection of stimulus involved in sensory perception"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042045">
        <property type="term" value="P:epithelial fluid transport"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0016322">
        <property type="term" value="P:neuron remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
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    </dbReference>
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        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
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        <property type="entry name" value="VDCC_alpha-1_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005446">
        <property type="entry name" value="VDCC_L_a1su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002077">
        <property type="entry name" value="VDCCAlpha1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45628">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45628:SF1">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL TYPE D SUBUNIT ALPHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00167">
        <property type="entry name" value="CACHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01630">
        <property type="entry name" value="LVDCCALPHA1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 15 16" id="KW-0106">Calcium</keyword>
    <keyword evidence="4 16" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="3 16" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="13" id="KW-0407">Ion channel</keyword>
    <keyword evidence="11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="9 16" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="115"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="153"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="186"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="246"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
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            <begin position="324"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
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            <begin position="357"/>
            <end position="379"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="486"/>
            <end position="503"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="523"/>
            <end position="545"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="609"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="678"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="849"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="892"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="925"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="971"/>
            <end position="992"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1004"/>
            <end position="1025"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="19">
        <location>
            <begin position="113"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="19">
        <location>
            <begin position="485"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="19">
        <location>
            <begin position="853"/>
            <end position="1005"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="727"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="730"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="740"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
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            <end position="802"/>
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    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="338"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="668"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00061395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR602077-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU003808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON012434-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1026" mass="115042" checksum="007F8797D3857348" modified="2023-06-28" version="2">MSQATAKMDDTELVNSTIRSEVTCPNHNISGTAIGTTNIQSGGDGVAAAASEPIGATGAGAINIGGTTIPIAPKKPTRRAGVKPQPDRPMRALFCLTRTNPLRKLCIAIVEWKPFEYLILLTIFANCVALAVYTPFPNSDSNSTNAALEKIEYIFLVIFTAECIMKLIAYGFILHPGSYLRNGWNILDFTIVVIGMISTALSNLMKEGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSILRAMVPLLHIALLVLFVIIIYAIIGLELFSGKLHKSCFHNETGEIMDDPHPCGEDGFHCDTISPEMVCRYYWEGPNFGITNFDNFGLSMLTVFQCVTLEGWTDMLYYIEDAMGSSWQWVYFISMVILGAFFVMNLILGVLSGEFSKERTKAKNRGDFQKLREKQQIEEDLRGYLDWITQAEDIDPDNDASGMQEGKMKNIIELDSSDNVGEDGEIQHESWVARKRKTIDRINRRLRRACRKAVKSQAFYWLIIILVFLNTGVLATEHYRQPPWLDDFQEYTNMFFVALFTMEMLLKMYSLGFQGYFVSLFNRFDCFVVIGSIGEMILTSTQIMPPLGVSVLRCVRLLRVFKVTKYWQSLSNLVASLLNSIQSIASLLLLLFLFIVIFALLGMQVFGGKFNFNSSVDKPRSNFDSFVQSLLTVFQILTGEDWNMVMYDGIQAYGGVASLGIIASIYFIILFICGNYILLNVFLAIAVDNLADADSLTTVEKEEGDNPDGEEEKLSHEPTPTEHGDDGFMDHDKDNLDSDNDPMNISDDYDGQDSDTKIPVAEDDEGYEEQDTPDSQDGGLKVARPRRMSELNVINTILPIPDGSAFFILSKTNRFRIFCHWLCNHSTFGNIILVCIMFSSAMLAAEDPLNANSERNQILNYFDYFFTSVFTIELLLKLVSYGFLFHDGAFCRSAFNLLDLLVVCVSLISMFFSSGAISVIKILRVLRVLRPLRAINRAKGLKYVVKCVIVAIKTIGNIMLVTYLLQFMFAVIGVQLFKVIDSSCIGCYCMTLAVLFFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W8E3</accession>
    <name>A0A6E8W8E3_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Max-binding protein MNT</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Myc antagonist MNT</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Binds DNA as a heterodimer with MAX and represses transcription. Binds to the canonical E box sequence 5'-CACGTG-3' and, with higher affinity, to 5'-CACGCG-3'.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a homodimer or a heterodimer with MAX.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8W8E3"/>
    <dbReference type="EnsemblMetazoa" id="ACON013301-RA">
        <property type="protein sequence ID" value="ACON013301-PA"/>
        <property type="gene ID" value="ACON013301"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON013301-RB">
        <property type="protein sequence ID" value="ACON013301-PB"/>
        <property type="gene ID" value="ACON013301"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011154"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON013301"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042400"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11402">
        <property type="entry name" value="bHLHzip_Mnt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000034">
        <property type="entry name" value="MAX network transcriptional repressor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11969">
        <property type="entry name" value="MAX DIMERIZATION, MAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11969:SF99">
        <property type="entry name" value="MAX-BINDING PROTEIN MNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="13">
        <location>
            <begin position="530"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="18"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="308"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="409"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="625"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="696"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="875"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="579"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="18"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="308"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="319"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="343"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="709"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="888"/>
            <end position="913"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00057176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00062701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00070444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00083368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON013301-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1070" mass="106605" checksum="4082F81855C5E384" modified="2020-08-12" version="1">MLSAVDTLLEAAKFLEQQEQQPPHLQLQPASRHHRTPPPLHRQTGSNVAYATTIVGAASLGGLFGGSSTGGGGGGGGIAFANGASNGVVVTGGSHHITNNGTTTITSISSSRNGNGFSSPPPAGTTVSLKSKVLLNGHHQPPTLVQNGGSTAAAASRLLSPTLVSLPASTTATLSAVSSTAHPHLGAASVVNATATRQPLLPAGARKRTYSNASNGATTIGLGSVSVNGHSTAAATTTTTTVLVGTGSMAGYVKDEPLTNGHSTAPPLVVIATNGTQQHPAAKHHLTLNHVAVPIAPSSIPAGAPTTITTTSPSPMTIDLSTKGLSQHQQTSTTHYAAAAVHPSPLSTTPPATTTTVSSLSSSSSSSSSSSSSSSSSSSPSSTTLTVSPVDDSQSSLNLSNGSSINGYITSSSSSSSSNSSGAAGAPIGGSSPLPPGLAGMLPINITNVLQASAIAAAHNGGTGSSTPNGTIVTAAAATGVHHLPLELQQQVAAGLSVAAISSQGAQGLFANIPTGTRRRTTSSNSNGVGTREVHNKLEKNRRAHLKECFEQLKKQLSLQPDEKKISNLSILHAAIRHIQLLKRKEREYEHEMERLAKEKIASQNRILVLKRELTQMGDIDVSRLAPDTDITPNPTNGGTVVPPNGLQMERDQPSDTIPGRNGIRYSSSSSLSSIATNASISNLPLQTTISPVLSVSSPSRASPALNRSSTSPASSLSSASPSSSAASSLSVSSAAAASLMAPLSLTTKGTAGTEPAAHNGLKITAAGQLNGLNLTSASLAMVNGANVTLNGTTAATTAPLPVIVGANGTANIVGIAHSQLLAASTAGGSSPAAIQTIPLNLNVTSSAVSNGLVTGSGPASLAIATAGAGGPLQLTSHNGGPIGGGSISSSSSSSSSSSHSSSNTSSSSTGAANTTTVVGTIARSMYSAGTIAPSMPTSTAAATTMVHVPTGGSKELINGTIGKNGTVRTDNSKLEIIASQSQPTNHSTQAVVGATALDPPGTKVITIMNGNTFALAPLDKDSKMGVVYSPILVPTSQGLRMIQQGSNGLATIELAPPNGGNRLQLHLTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WA65</accession>
    <name>A0A6E8WA65_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8WA65"/>
    <dbReference type="EnsemblMetazoa" id="ACON013481-RE">
        <property type="protein sequence ID" value="ACON013481-PE"/>
        <property type="gene ID" value="ACON013481"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009241"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON013481"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030170"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON013481-PE"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="356" mass="41147" checksum="78CF457050369953" modified="2020-08-12" version="1">MVYESDFYSTRRVGSSYTRPTISSYTVTTPLRYSGVPRFNTITTTTTTYRTPMPYVAHKRLVPVTRVVTSPPRIVVSPMRVLGSPVRVISSPVRVLGSPMRTVRAIRSPARVIVSPARVVTIRSPYLRPSIINKEFDRIERKYRASPVSSALEQYYNSPSYLEFEDAKSDIRNSTALLLRQLNDPVPRLMAPIAQAAPEPNPKKWVYDPFSTHNRLNSDTYVKSHITDPIRSVRNDIEAMARYHSPASRYVEYVLLDDILWRYTYYVDEEGKNHLASTRIIGSQAYPKTKPRIYNYDTAKVGKDVNVMSYYKSNRTQAKADVEEVNTKIKKYLEKYYLNHAYKVPDEFKEFLHEYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WA72</accession>
    <name>A0A6E8WA72_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="5">PDZ domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="EnsemblMetazoa" id="ACON029509-RA">
        <property type="protein sequence ID" value="ACON029509-PA"/>
        <property type="gene ID" value="ACON029509"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007103"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON029509"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042667"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd00136">
        <property type="entry name" value="PDZ_canonical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47644">
        <property type="entry name" value="AGAP008221-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47644:SF1">
        <property type="entry name" value="PDZ DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="4">
        <location>
            <begin position="1904"/>
            <end position="1981"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="3">
        <location>
            <begin position="1994"/>
            <end position="2092"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="3">
        <location>
            <begin position="2114"/>
            <end position="2211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="255"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="474"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="578"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="712"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="792"/>
            <end position="1114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1138"/>
            <end position="1373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1386"/>
            <end position="1659"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1762"/>
            <end position="1801"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1845"/>
            <end position="1867"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="339"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="255"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="503"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="526"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="587"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="729"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="815"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="836"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="857"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1037"/>
            <end position="1053"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1071"/>
            <end position="1095"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1103"/>
            <end position="1112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1138"/>
            <end position="1147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1224"/>
            <end position="1242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1243"/>
            <end position="1267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="1310"/>
            <end position="1322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1356"/>
            <end position="1373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="1400"/>
            <end position="1410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1411"/>
            <end position="1432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1454"/>
            <end position="1472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1491"/>
            <end position="1500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1507"/>
            <end position="1516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1553"/>
            <end position="1566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1603"/>
            <end position="1620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1621"/>
            <end position="1643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1773"/>
            <end position="1789"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON029509-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="2217" mass="244739" checksum="7A0F3F1B43097C0E" modified="2020-08-12" version="1">MGRKKGRPFETGPCSRAPVYSKGDIREQYCLTDRQLNSIEQPRRERFFGCWSGRNAPQSFLGVCVGRRAPSDENLADYAPMQRYPRYINSGPIAKSILQQYDDDMESSYFRRKPTSFLSNSSQGGGGGAGGGGSAGLIIGSSATLPAGGDSKRYGGSWLPAEDEAVRLEGPRTKHVSFARSYTLTSFDEAMGSRPLSRLAAARSQERLIGGKKPTITLAQHAAGPYATGAPLHSILTQPTAQPQPLLPQALQKQPLSVPQNPSHSQPQQPPHQQQQQPHHQPQQQQPQQPQHVLIQQQPTSHLHQHLHQQHLQHLQQQQQQQQQAALLAHQAHLQHQHQQHQQQQLQAAQLAAQQQQQQLQQQQQQHQQEMVVLEKVKRSAMKTQATQTEVYLGKKGTAPHNLSLSPRTIHRVKMVSQGAQTNGGLNGGGRKLTKSLSEAGDRLQENGIDPNVLQPVDTGTSANANFDHELLHRTQSEEPPKSPFTITSPPATHVGPHFELPSSRTSSQLSHSDGTHLTHSRNHSQDSATSYRSTDGSRTSYETASYPYDAYDSIVLPRRASHSQEPTFQFLRKQDLEQVHHHHQQHQQQQQQQQHLQLQQQQHQQQHQSQYTHGYENGYVPFETHSLPRRTCIHHKPEEFHTHTLPRRDHGHAHHHHDIVYRDQSHPALPAPEHLILSNGSVNQDVRSFDSMPTATALNRRMSAHAIVLPPEKQPHSSGLPLVQGLHQQQQQQQQQQQQQQQQQQQRRASYAFVTPDTQALAKRNSIPHQQIQKVQPIMQTQFAAPAKTQPFQPLVKPQPPTLPSHYTSQTAPPSCPVTPPNIQPLDMQDDTKSSSEGSCSTCDSDSEQELDGMPGEEREIFIDFKPRVSPVPSPGAVKKKKLQKAQSEGELMHADARRKASVTADGDGGSSTVPTGSDDEGRSTMREQCAAPKLPKPDYRDASESSVPDDEPQVKDRRKESFRKRSVSLDQPTSCEQDEEEEREHNGSVVQQHPHRNGGDSGRAHPHAAQRMSSRSRSIGAAIGSRHLSAPPSPQKEDHSASTGRLHREASPFHSSDSLANDATRDTSDGNWNESQATIMTQSLTDNGKLTPSSRRKNLLLQHQQRSSMDTDVLDMEDIADQPLPPLPVTVASTALQSNTVSTPSGHGHQPNGMAIQPSVAGIGIVKPTTPSIAVIPSPSMEREERDLQWQKSATLRRPSRPLSPTKRAARSEQQKQQQKLHKIEPLIHRSSDPTERTGKSDPSPTSSMPSGSPATPQQPSPSSTKLPHHHPIGSATPVAARFAKGSPGNGVDLLKDHPVFSQETGTSGPGGATGGGAGSEGSTTEDYVTCTDNSKRGASGAKGTYKTIHATRPTKQVPATTQDGSSFESASSIYSLARVDAVCEETPPVEEKSVSPQIPPPPIPKPSPTHSVSSSSSDSFSVSKKCSPSRAALPKAIAGKVKPAKPQSLSDDERSEKRYSSSHDEKEAAPKLPYAGAATGTIARSARRGRDWNEEERRRKKSTFKLEFDKDSIKTYTTPMLRQPSPGFKKLSPEDKSALNVLDGASPSSKQKRFRPKTRKSPRARSAVPEDTTAGSGMGSTGTLPRRSKTPLVRSPEKPTVAAAAAATATTAVVMATPQPTIVTTGTKLSPSQYYSQIRSPPSVGSPRKQKISPEKRLQAISTESLRSVSPGSDSVFYSEADALSHCEHQVHCSHCGKEVEVSNLPGESEESILTLDSTDNDRPDIVQPPEGFADSPDCTKTPHHTRLYKKMDKRFRSEDRHVDRRHFKSRQEYRAKSEERGKEEIEPNNLRPAGSSPCVAPAGQAYIDTTSEPEQGIYHGNYKAGLWICIADRDVWRRNELPDGSFDRPKDATLERRGSTDSERDFRKKYQAITHRLVHRKSCVEMYRRQNSNSFDTDKTVVVCRKSGEFGFRIHGSKPVVVSAIEPDTPAETSGLEVGDIVLSVNGISVIDKSHSEVVKIAHAGSDTLELEVARTIGMLSPLDDGSSHPSIYTGFLWRYCSSGGKWVRRWFSLRPDHCLYYYKSDADNQPIGAIMLTNHTIERIPLEPITRPHSFAIDTEEGTKVQLSADTEEAASRWIAIISHAAQQCDPWLENSARNLRLCATGISKPDCSGPLTKLGSKWRSWTKRYCVLKDAVLYFYHDGTSKSAFGMACLQGYRVQPSSAGGKKYAFEIVPPELSLRHYYFHTETEMDKKRWVAALEYSIDRWMRAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WA88</accession>
    <name>A0A6E8WA88_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Cyclic nucleotide-binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8WA88"/>
    <dbReference type="EnsemblMetazoa" id="ACON029523-RB">
        <property type="protein sequence ID" value="ACON029523-PB"/>
        <property type="gene ID" value="ACON029523"/>
    </dbReference>
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    <dbReference type="VEuPathDB" id="VectorBase:ACON029523"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032177"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034702">
        <property type="term" value="C:monoatomic ion channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005242">
        <property type="term" value="F:inward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000020">
        <property type="entry name" value="Potassium channel, voltage-gated eag-related subfamily H, member 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000011">
        <property type="entry name" value="Potassium channel, voltage-gated eag-related subfamily H, member 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1200.260:FF:000001">
        <property type="entry name" value="Potassium voltage-gated channel subfamily H member 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003938">
        <property type="entry name" value="K_chnl_volt-dep_EAG/ELK/ERG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003967">
        <property type="entry name" value="K_chnl_volt-dep_ERG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050818">
        <property type="entry name" value="KCNH_animal-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217:SF548">
        <property type="entry name" value="GH12235P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217">
        <property type="entry name" value="VOLTAGE AND LIGAND GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01463">
        <property type="entry name" value="EAGCHANLFMLY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01470">
        <property type="entry name" value="ERGCHANNEL"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0630">Potassium</keyword>
    <keyword evidence="6" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="362"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="509"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="577"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="602"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="15">
        <location>
            <begin position="705"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="287"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="861"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1083"/>
            <end position="1103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="875"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="904"/>
            <end position="915"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON029523-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1164" mass="130348" checksum="ABCF2D0CC00BC250" modified="2020-08-12" version="1">MSEASLRANSCVELSEIRKLDSLLRQCETLSYDDLSSGSNGERELEDYMEQRKHVRRRRNSSGNCIFTPPINKSTLSMLSSLNILNGARRIMGAMNGPGGLKTGTNNDDCWLLSQKSFTTKSCSNLPKGMCFHPSAGLGPIGKPLHQQPVDNLCSLSETILSNLRKAAHAVHVKPANRETCGCSTSSPNPNPPNTLQLPIGVPATVEQSCEDSGKSVYRKNQCLAPDEDNRIVNIIETPNIVPISMKSLNSALSERLCYTTKAQKSFDNKSDKSLLSQANSVKSRSKFSPLSRKVSHKTNSFDTEPTEGAKESLLGHKFEKILPEPVETVLSLGADVLPEYKLQSPRVHKWTILHYSPFKAVWDWIILLLVMYTAIFTPYVAAFLLSEPDYNQRKNRKYADDPIVIIDLIVDVTFVVDILINFRTTFVNGQDEVVSHPGRIAVHYLSGWFLIDLVAAIPFDLLLVGSDTDELGLDKDETTTLIGLLKTARLLRLVRVARKIDRYSEYGAAVLVLLMATFALIAHWLACIWYAIGNAERPLLKAKIGWLDALAQDTQEYYFPNNTGGGPSVKSRYVTALYFTFTSLTSVGFGNVAPNTDAEKIFTICVMLVGSLMYASIFGNVSAIIQRLYSGTARYHTQMLRVREFIRFHQIPNPLRQRLEEYFQHAWTYTNGIDMNSVLKGFPECLQADICLHLNRNLLNNCSAFEAASPGCLRALSLKFKTTHAPPGDILVHKGDVLTYLYFIARGSIEILKDDVVMAILGKDDIFGENPCIHSTLGKSNSNVKALTYCDLHKIHRDDLLDVLDLFPEFYDSFVNSLEITYNMRDEEQAGVELRHRYMRTGSQDRESETRSYVRKLNTVHHRPPGNKCDMPNDRSSIGQMSTNYDDDRKFSLSASPNESPKSTHKQTIPHHHEHQSSAIVASSAALGRAPDTVTLSAVSHRACTCGSKADVPVGPMRVSDCSPKSSPDEEIFHHTAPNSSNMSKPDESKQNLETMAHQLSELTNRIGVLETSLKHDIRTILEILHQQQQMQMQIQHQQHSFAQQQQQMHQMHTGKTAMSSYQPSESDFSFDMCGPPMDCREVKHQQPPSSQHRIHVARSVSQPECTDDRSLFKCSKFSSFNHPMDDTPEGQNWNIFAPIAKLESLDEIDQETKPSSSHDKMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WA94</accession>
    <name>A0A6E8WA94_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Harbinger transposase-derived nuclease</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="1">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8WA94"/>
    <dbReference type="EnsemblMetazoa" id="ACON013250-RB">
        <property type="protein sequence ID" value="ACON013250-PB"/>
        <property type="gene ID" value="ACON013250"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007371"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON013250"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035336"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON013250-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="126" mass="14183" checksum="37A8FC2D942F79B7" modified="2020-08-12" version="1">MDNSTVSEMPAASEEISDETVRELLGMSEEDFNYLLNEISGKISRRDTFMRKAFTAKERLIVTLRFLATGESFMALASLYDISASSIRTIIPEVCECLIKSLKRYVQSNCHGYLTPATVSRTQSVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WA99</accession>
    <name>A0A6E8WA99_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">May be involved in the metabolism of insect hormones and in the breakdown of synthetic insecticides.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 14">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Endoplasmic reticulum membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Microsome membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 15">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8WA99"/>
    <dbReference type="EnsemblMetazoa" id="ACON013511-RC">
        <property type="protein sequence ID" value="ACON013511-PC"/>
        <property type="gene ID" value="ACON013511"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009977"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON013511"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037074"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050476">
        <property type="entry name" value="Insect_CytP450_Detox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24292">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24292:SF84">
        <property type="entry name" value="CYTOCHROME P450 28A5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 14 15" id="KW-0349">Heme</keyword>
    <keyword evidence="11 14 15" id="KW-0408">Iron</keyword>
    <keyword evidence="13 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 14 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0492">Microsome</keyword>
    <keyword evidence="12 15" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="10 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="98"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="14">
        <location>
            <position position="307"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON013511-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="19">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="363" mass="41567" checksum="46C57F850373FDBD" modified="2020-08-12" version="1">MLLILFGTVVAFVALVYIFLTWEFGFWEKYGVKGPKPKILLGNIPYLLTKKRHTVYNYDQLYVMAKYTTDVVASCIFAIDAQSFVKENPEIRLMGKRIMNFNFVVQLALLVTTFFPSDAIRYRKENKINRTDFLDHLLQLQDRKQITDIDIAGHGVSFFADGFETSSIVLTYCLYDLAKYPTIQQELRDEIAKAKQAGGGTMLYEQLGEMALLEQIICESMRIHPLMAVMAKRCTADTTLVGPKDRKIFVKKGTTVVLPYYSICFDAEHFADPYKYDPKRFAAENGGSKAYRERGVYFPFGDGPRMCLGMRFAVTQVRRAIVEVVERFSISVSSKTIEPFEMDPDQFMLMPKGSIWLDFKPIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WBY7</accession>
    <name>A0A6E8WBY7_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8WBY7"/>
    <dbReference type="EnsemblMetazoa" id="ACON029446-RC">
        <property type="protein sequence ID" value="ACON029446-PC"/>
        <property type="gene ID" value="ACON029446"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005351"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON029446"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040499"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019898">
        <property type="term" value="C:extrinsic component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13241">
        <property type="entry name" value="PH2_Kalirin_Trio_p63RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.900.10:FF:000008">
        <property type="entry name" value="rho guanine nucleotide exchange factor 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001331">
        <property type="entry name" value="GDS_CDC24_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051336">
        <property type="entry name" value="RhoGEF_Guanine_NuclExch_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055251">
        <property type="entry name" value="SOS1_NGEF_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22826">
        <property type="entry name" value="RHO GUANINE EXCHANGE FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22826:SF106">
        <property type="entry name" value="TRIO, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22697">
        <property type="entry name" value="SOS1_NGEF_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00741">
        <property type="entry name" value="DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="7">
        <location>
            <begin position="78"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="DH" evidence="9">
        <location>
            <begin position="404"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="8">
        <location>
            <begin position="604"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="143"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="238"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON029446-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="743" mass="81230" checksum="50435FBB7B75E300" modified="2020-08-12" version="1">MSKASNNVDKGGGGGDVDGADRSSAKGGGLSSTGSTKKSGRKISLPWFRQSSVPSHAALARQHTIDSPGSFQFFRKGGLAEMTWVIADHTAAPGTSELSVSKGQQVEIVDMNCSGAPDYCLVRLSINASDSQEGLVPVTVLKPLPSSHSKLNNKQRDTDTLGEVPQESDCILPNQHTSPVNKRKGFSGKKWLPTQFRKLSHGKSDKLPPVEKTLISKKGSDKKVKPNQQEKPPEEQESSAAASAAQLEPEDEEATAIELPPPMKPIQDTASALSGASVVPSSAEDAAAGSTNKITKAMSLKSLEEPVGAQPPADLAEIEQFVKAKVESLKYLDNDGGGGSSSSSAAAALAMGLMVGDGSSSSGTMALNGGHTIAKPAISPQFSENEAADDTLTEEQCIEDILRKRNYALRELLTTEEAYVNDLSQIVNGYIAEIRNPASTVLIPDDLKGGKERMVFGNIEAIYEWHRDHFLKALQKCLQNPYELGPLIKRSERKLHMYVVYCQNKPVSEHIVQEHMSYFDELRLKLKYKLCLGDMLIKPVQRIMKYELLLKDILKHTLRAGLTEEVPGLKEAMHIMQVVPKAANDMMDVGRLQKFEGKITTQGKLLLHGPLYCVEGASSSDKNSYNSQKPKELHVFLFEQNIIFAEIVGKKTQFTSPNYIYKAHIQVNKMTLQDLSDQTNGNRFSLCSIDPQRSSLSYICTAPTPELHSEWLNTIHEILKTQNDFLKALVSPIAYQKELTKDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WDQ3</accession>
    <name>A0A6E8WDQ3_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">FYVE-type zinc finger domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8WDQ3"/>
    <dbReference type="EnsemblMetazoa" id="ACON029512-RA">
        <property type="protein sequence ID" value="ACON029512-PA"/>
        <property type="gene ID" value="ACON029512"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004660"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON029512"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032043"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="205"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="311"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="420"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="531"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="699"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="753"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="845"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1001"/>
            <end position="1061"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="314"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="506"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="549"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="699"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="789"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1001"/>
            <end position="1020"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON029512-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1078" mass="116855" checksum="C7FCAA2CD513F887" modified="2020-08-12" version="1">MVFCSCSFLAYYLLTAVVSYVAVYVLQVVLDLPLWEGQQQHHSPSDQHFELKRLIQKILEEIAKLPELLNHSGLPLRPEEHLPYFDPKKYEQLLATAVLNKVVDDYRNPKNFTDGGDVAGKGQTGAGATPYTGTTVDINHNQLSETGSSRSLLSKESLRQMSVTADDQAPQPHEGVSYSAIGTERRLSDTDESYLSDYIQRHKVPLPDLSDTTGSGSGAEDDDLQSLKSNATDGTWEENWLFRKRQLKTTESSIAMLVPSPTEEVKALIGDKNADEISDLSEAGSDCEGYESDGNVNGGTVPSVAPVVVASSSKDTTSEGISSSSKTQSLDEILPPDSLVSINSLPVNEEALSEATNSQLLADQVAQNGASERLQVDDLISIEPVADRVETTNPTLTNPFLDDVFEANNNVITDDVKMLQRTETGDNRSSATESNGYGDRKEIPIDRAQIATNGGDVPEVQGDSGEQDASVDAPDTLIPGSIAEREHLKWRNAKPIANNPYSPDVLQRRLSEKTRPSSMIEIDRLVRKEAAPATGRAAGGVLIDDEETPKEPTDSGTENDRSLQSVTGGVLTETKKIGREYYINDPERLRAGGAPLKSTLGPQTQTQQPSHSTDDEKSLLLGRAVDESEAAAPQKLTPEPVSSLYRSSSVGKQRDPRAEDIFAARPVQVTADDPILQQGTKVSYLSTAAREIYLVPVESEPNGGSLMSSSSELSSVHSPTNAHQRPELDSLTYSEDSDVTRIYDLTTGEAKVVRSCHSETEPPHDTILQILPQPSPTKQQPATMATAPSHEQLSSSNVSALKSQLSTGERADAGSYHRRSPFPVKPLSPETIKFFAPKRKLSFTGSTNSLVGESGSKGAGSEQGTTHVLPSMHSSLHIDYPASRSTTGSEFAIIEKDVIDVLPSVKELAKCYSGSTSDVSTLPPKPLYKPRDFIRQSSDVLNEEGMPNTKGQRQYSSTSSIAVRDEIREIRKLNLEAYRQSTYYPMAPGHSITARSLSKQIREHKSNVTDDHKVGHHQQEEQAAPAPLVPGGADGELNGTDADDEPGHASPERPASPVLLPGHLKSSIQFFESLKNKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WDR0</accession>
    <name>A0A6E8WDR0_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">P-type Cu(+) transporter</fullName>
            <ecNumber evidence="3">7.2.2.8</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="17">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.8" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8WDR0"/>
    <dbReference type="EnsemblMetazoa" id="ACON029519-RC">
        <property type="protein sequence ID" value="ACON029519-PC"/>
        <property type="gene ID" value="ACON029519"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000618"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON029519"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032853"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043682">
        <property type="term" value="F:P-type divalent copper transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140581">
        <property type="term" value="F:P-type monovalent copper transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055070">
        <property type="term" value="P:copper ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015677">
        <property type="term" value="P:copper ion import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="CDD" id="cd02094">
        <property type="entry name" value="P-type_ATPase_Cu-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000150">
        <property type="entry name" value="ATP7, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000001">
        <property type="entry name" value="ATPase copper transporting beta"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000002">
        <property type="entry name" value="Copper-transporting ATPase 1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000031">
        <property type="entry name" value="Probable copper-transporting ATPase HMA5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017969">
        <property type="entry name" value="Heavy-metal-associated_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006122">
        <property type="entry name" value="HMA_Cu_ion-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027256">
        <property type="entry name" value="P-typ_ATPase_IB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01525">
        <property type="entry name" value="ATPase-IB_hvy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00003">
        <property type="entry name" value="copper ion binding protein"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43520">
        <property type="entry name" value="ATP7, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43520:SF8">
        <property type="entry name" value="P-TYPE CU(+) TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00942">
        <property type="entry name" value="CUATPASEI"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01047">
        <property type="entry name" value="HMA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0186">Copper</keyword>
    <keyword evidence="9" id="KW-0187">Copper transport</keyword>
    <keyword evidence="15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="16 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="404"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="446"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="480"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="509"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="669"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="714"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1123"/>
            <end position="1145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1151"/>
            <end position="1171"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="19">
        <location>
            <begin position="28"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="19">
        <location>
            <begin position="128"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="19">
        <location>
            <begin position="237"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="19">
        <location>
            <begin position="313"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="196"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="196"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU362081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON029519-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1270" mass="137036" checksum="AA3CE67EA45F4D5D" modified="2020-08-12" version="1">MWNNGYGTSRENMDDYGESTGLLEQPVASVRLPIIGMTCQSCVRNIEGTIGSKLGVIKINVVLAENAGYIDYDPSLTDPAQLAADIDDMGFECTYTEPGASSAGPKRSILVEGVESDSETTNQKSDVRTTRISIEGMTCQSCVRNIEGNMKDRPGVISIRVLLDERLGIVEYDGRQTTAEAIAEQIDDMGFEARVAGEQHQPEQQRARSSPQANGKKHATNGNLTPKKKADESALLRRCFLHVQGMTCASCVSAIEKHCRKIYGVESILIALLAAKAEVKYDERLTTPADVAKSITELGFPTEVLEEPGTGETDVEIEILGMTCGSCVAKIEQTALKIPGVLQASVALTLKRGRFKFNNERTGARTICEAIEGLGFATRVLSGKDKMAHNYLEHKEEIRKWRNAFLVSLVFGGPCMIAMVYFMVLMHERSHEEMCCVLPGLSLENLIMFTLSTPVQFVGGWHFYIQAYRAVKHGASNMDVLITMATTVSYLYSVGVLVAAMVLEQHTSPLTFFDTPPMLFIFISLGRWMEHIAKGKTSEALSKLLSLKATEATLVTLGPDYAVLSEKVISVDLVQRGDILKVVPGSKVPVDGKILCGNSTCDESLITGESMPVPKKKGAVVIGGSINQNGLLLMQATHTGEHTTLAQIVKLVEEAQTSKAPIQQLADRIAGYFVPFVVAVSVITLVGWIVSGYIDIGHIPASDRDKEGLTPSEIIVSYAFRCALSVLAIACPCALGLATPTAVMVSTGVGALHGILVKGAGPLENAHKVKTIVFDKTGTITHGMPMTSRICMLVKPAVCSLARALTIVGSAEVNSEHPIATAIVKYVKETLEIDGFGRCSNFSAVPGCGIRCVISNVGDTLKRVQQSEKMRNYQNSYRSEQQQQPLFMNGAVVEELIPQLSASQKNTIELQQLLHLEPIGEGPAGTGGDAGSGEAFADVNEYNVLIGNREWMARNAIVVPPEVNIRMSEEEQMGHTAILCAMNGQLVCMLSVSDMVKPEAHLAVYTLKRMGIEVILLTGDNKNTAASIARQVGINRVFAEVLPSHKVAKIQRIQEMGMRVAMVGDGVNDSPALAQADVGIAIASGTDVAAEAADVVLMRNDLLDVVACLDLSRKTVRKIHLNFLFASMYNLLGIPLAAGIFTPFGFTLEPWMASAAMAASSVSVVCSSLMIKLYKKPTRASLQTPEYVELMEAGAFLDPDTISVHRGLDDIPRPNLARSTSSTLAKLFGRGKDTKEDGLLATLDDDEFSVGIVKTSNASIYKDATELQKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WE20</accession>
    <name>A0A6E8WE20_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="13">RING-type domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8WE20"/>
    <dbReference type="EnsemblMetazoa" id="ACON029666-RB">
        <property type="protein sequence ID" value="ACON029666-PB"/>
        <property type="gene ID" value="ACON029666"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005865"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON029666"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042043"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02122">
        <property type="entry name" value="PA_GRAIL_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16668">
        <property type="entry name" value="RING-H2_RNF130-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.30:FF:000003">
        <property type="entry name" value="E3 ubiquitin-protein ligase RNF128"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000009">
        <property type="entry name" value="E3 ubiquitin-protein ligase RNF130"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46539">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE ATL42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46539:SF23">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="2 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026194452" evidence="11">
        <location>
            <begin position="27"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="230"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="12">
        <location>
            <begin position="297"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="368"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="448"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="489"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="375"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="502"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON029666-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="530" mass="57532" checksum="B4810D196743BD24" modified="2020-08-12" version="1">MMSALSHQVNMNNFLSLLVGASLVAANSSPSHDIASLSQPTEFSNGVSSFALPAEDRMAIESSTLAYLNYSFTGPDGHLIEFGSESAKYGEGRVLNRSGLLVHISNSANHSDHTGCSKQWSGTLGGTIPERDAPWIALVRRGSCNFEDKVKHAYERGAAGIIIYNDKNDTKLEKMKINDKERNITAVFTTNAMGRELIEILEVHRSVVQMRIIEGSRQFRNLGNINRTSVLFVSISFIVLMIISLVWLVFYYVQRFRYLQTKDKQSKRLCSVAKRIIAKIPTKSIKSDDKEIDNDCCAICIEPYKVTDVIRVLPCKHEFHKVCIDPWLLEHRTCPMCKMDILKHYGFVVGSSSSSQINSAIAEYLNVAPPPPPSNTSTITTSSIASTEESSRRVSWSTGADMGPATSATVDGSIPTTLFGNDYVAGRNGGTSVGQLTDIVTIIINIDDNESPTGRGDTVTGSSGASGGGSNSRMGVLCDLSTHDVVNCSSASASSNDRDNSNCDNGNTISNDSNESNSDSHLISRKITDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9I3B206</accession>
    <name>A0A9I3B206_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="3">MGA conserved domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2023-03" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="EnsemblMetazoa" id="ACON010686-RB">
        <property type="protein sequence ID" value="ACON010686-PB"/>
        <property type="gene ID" value="ACON010686"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030989"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032060">
        <property type="entry name" value="MGA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039191">
        <property type="entry name" value="Nopp140-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23216">
        <property type="entry name" value="NUCLEOLAR AND COILED-BODY PHOSPHOPROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23216:SF1">
        <property type="entry name" value="NUCLEOLAR AND COILED-BODY PHOSPHOPROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16059">
        <property type="entry name" value="MGA_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MGA conserved" evidence="2">
        <location>
            <begin position="1035"/>
            <end position="1088"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="117"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="235"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="383"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="704"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="965"/>
            <end position="1000"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1015"/>
            <end position="1038"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1279"/>
            <end position="1304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1322"/>
            <end position="1426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1491"/>
            <end position="1514"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1624"/>
            <end position="1669"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1858"/>
            <end position="1907"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2112"/>
            <end position="2191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2222"/>
            <end position="2253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2400"/>
            <end position="2444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2558"/>
            <end position="2614"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2859"/>
            <end position="2966"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="118"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="387"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="704"/>
            <end position="715"/>
        </location>
    </feature>
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        <location>
            <begin position="980"/>
            <end position="995"/>
        </location>
    </feature>
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            <begin position="1350"/>
            <end position="1363"/>
        </location>
    </feature>
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        <location>
            <begin position="1398"/>
            <end position="1407"/>
        </location>
    </feature>
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        <location>
            <begin position="1491"/>
            <end position="1503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1627"/>
            <end position="1637"/>
        </location>
    </feature>
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        <location>
            <begin position="1866"/>
            <end position="1893"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1894"/>
            <end position="1907"/>
        </location>
    </feature>
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        <location>
            <begin position="2151"/>
            <end position="2168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="2407"/>
            <end position="2423"/>
        </location>
    </feature>
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        <location>
            <begin position="2573"/>
            <end position="2607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="2878"/>
            <end position="2901"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2902"/>
            <end position="2935"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="2936"/>
            <end position="2951"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010686-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="2966" mass="325664" checksum="802BD7761766DA6D" modified="2023-06-28" version="1">MILSNTYKPSPPCECPSFSCWLGRKSSVLRADSAPPVYPLASVPRAKDADPKAYPCRLRICRLMYKPLPQGSCKSDLRQHRNRRLALVKKRAIRLVQTFAARRAIPHYFQKRVKHTLSAKQSASTHNGATVGSTDAAASEVPVNSNAITKESKEERRDISQVREEEPSSAAPQEQRIDPPSEESTALVRATPKTTSHTDRVDTLSTIKEYYSIQCAIRLLLAMLQERMEEVESMQKSKVVDAPEDEAPLDNGSPKPIENGVEANGTSTEDDPAERGPLVKTEIDLSPNCSTEDADPNKENNVPLDSTLETMSPAAVVLNRVNSTPVKDEPRDDSRTYSFLETSKSDILDPTYTCDIELEPLEQRKNRSIVDSLLNKFNLSPPAKATVPSSFSDNSLDGSEGRRSLRDRTKIAARPRYIEIPEEPRKMRVSKVFGKLHKTLNLDLDCANSNSSTEFYGFDEGEIVKLDKPSLPGLLPTPIVKKSQPFAPFLAYGSSMDADSVETGAKTRSDLFREEGLISFGSLPPRLECPQRPNDMVRPRTVAQKRILVQRENDVRYLMIDNESKIFQFLKKRSKNIDAALDFQRMKELQDQQIPFTRDTWRALSWLRTEKGRYYFQTITIDNRTIKMSGCRGNHRYKTLSRNPLYSSPVVASHGHRIHYVSKCHCPPFPEGVKLNISEDRNKSSQSDAEERMMNDAARYLYRTTSNDTGYQNKRSYPGTKPGPLSSKCLQPSPDDDPTLGQLEVFKMPPVELEVFPKLNRPLDGFVKPYLKMILPHDGITENWARFAVSTLTADGAREEDKTVPSASEERSFVFELPYLNNQRRMLIRRRFMASPGSPVHCDVEEFRKLMDEKLTFRAAIDKALEEGKGDEIDPDELVCADVLSAITDSVAIALAEDILGKDDPEVDYVKWEDGARPSREQMTMPIKIETPASGAGGDVKPMSVKNEPPVSGAVGGAEKICTPPAPAASEVGSGVEITPAAEPSGPAPSSESGSKTVDPVKLKLLREMKRLNATIIEDPSDPAVSSAETTRQQKQQQQRCDPQYCSLGCICDVLHSSQPAAVAATDRRQHCRQSGCMFGCSCGFEEKLANQTQEVKTDLAAEGKGESTALTSADVKYLREKATARLAKEEREFTHTVILTKNTTVLVHNKETESRRQKKKPKKYDDYYNDLSMQSLLNGGSAKDVSQYISKPPPNAKPLSPADRMRHAHVLLTKLPQLADIEPLCMVHDLYRCFCHGKATQGKPFSFTEEGCLSPGKIFPSDGSYSVDHASLVLMPADSTSSGRMMGAKNRTPETAQPTYDTAPIASVRKRLYSFEKAYTEPAAHSSSSSSSDGKSPKGGLPVSRKRRDSSEESYKPPNERKVAKKKLHTSLEEKTATASASSLRARRASMVPGQVSARSVASTRRPSVAHAGQSASSARKSVPLAMRRSSVAHQKDDDVFKAPGQSQSTGTDAQYTVVKPKRASGGINNLILKRIPSNASFAPMVHLPSASSKATAKKSTPPSKPKDQGAAEIPVKYMSVRKLKQLMLSEYKNSPDVKKTPDDTSSTEVRLAFINILLLDTEENIDNEPQLNILSDGSKKLVINGNRRSFIARRAFKDGMPPERTMPYRNRTTYIVQILSASNGSEDESTESEEPAVEKAQSCESVPQMKNEPAKDLDDIEPYPEPPKLSAYAQRVSKAVTQHQNASSPIEQTDRQLCSLMRHINELLRRETLHINPAKKGIMHICRWNQLLNAFALAEIDVLDLTLTNGLEMTVIATAPFKQQLVPNVQHQISARRLTIDKMIVAERPSLLMKMVVCNVENVKTNELALVLYGNENFWHFCGFLKVDETSFKTDSNIPKAPSTIANSKIRTRLNDYQRKTVPATTAKTTTAQQSTKQATASKQTSSSSSSNSSHIEPAPSTNMLSTQSNIEIVPKAMQPVHFPLGLLPGIADTPEGGESDCRWMRIKIENDFSHMYLPSWQCCVTYGRIKKAIYDASRTAQAIRLDNPAPPNQPQRRQRPFMYALPKEGNALFLGPYKSSETKIDVILCQSVQGSLYEREVYERQNRIPMPANTKRTVGWWVDVVTSRVASVQGKIRQTAFNSFRPREAIKAPSGTVASTSCNTAQRSLLKRNNMPLAPATVGPEKQARQSITAVEKDQTPSASVPNDLDDDVVVLDSDDNDEGENLGTAKESTENSNTANEQPRREFAVEQIGIDLFRLVEGTMKQNQQEKREIEKVLKQERVTKSVSGDQNRNEPNRKRQSSPLPPAANYAPAAKVIRITDAAGVERTLPSGIRIPASTTIARNSFTRISLPGPQTLKSGSTTITRPINGVSTLTIAPLKSAESNVSVKVVQNALRVSRPSNAPPGVPVRALSTTKASLADELSGRQLVGVAGAPKPRPRLNSVAVERVSVQVSRPAPVVQESDDEDDVVWLDDDNESSNEKMKPPASTSGNADRTAASSTSVAPAATASVVVAGSNAINGVTAYAQKTILSQEQVAMIARRVDLTKTGQQDGYSFNPTSGLLKMKRSLLTSIENDAMLGEGTTCAKSAISAVVNGKMGEVRILSSNAKSLLPSVSSTSVNGKAVVGSSSSSSAKPSAIPAPASASLNASATPSPSSSSSSSTTITMPRTKVVPRVSVTAATARYGDVAGARRISDGGVGNSVIPPGSAGTAGGLLDNLKYLSEAQEEKLLGEHPFTRGVLESKIPGLGLVEVVRFEREVIIHMKELTVKKQLVSVHNLEAAVTLLNQFIQRNTYAFRPYNLAIQWKFKERSLPLRPEERMTSVINQFCIVTVYGIINVSKQEQLESVESTLPALYEDLMRMKLAMQCYNKQLYEDEKCYENDDKIYDRAVGIITSAKAKSKWLQQQRENLMKQRRANQAKLRQLKEASGSAEAKDSPEKSTQDKSATEQSAKDTSAKNAIAKNTTAKNATAKNVPARNTAARNSTARNSITRNSTAKNTPATDVSGSKGGQESVIVIDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9I3B208</accession>
    <name>A0A9I3B208_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phospholipase</fullName>
            <ecNumber evidence="9">3.1.4.4</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2023-03" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1,2-diacyl-sn-glycero-3-phosphate + choline + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14445"/>
            <dbReference type="ChEBI" id="CHEBI:15354"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="EC" id="3.1.4.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endomembrane system</location>
            <topology evidence="8">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the phospholipase D family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.4" evidence="9"/>
    <dbReference type="AlphaFoldDB" id="A0A9I3B208"/>
    <dbReference type="EnsemblMetazoa" id="ACON010687-RB">
        <property type="protein sequence ID" value="ACON010687-PB"/>
        <property type="gene ID" value="ACON010687"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON010687-RD">
        <property type="protein sequence ID" value="ACON010687-PD"/>
        <property type="gene ID" value="ACON010687"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032898"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004630">
        <property type="term" value="F:phospholipase D activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006654">
        <property type="term" value="P:phosphatidic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009395">
        <property type="term" value="P:phospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060627">
        <property type="term" value="P:regulation of vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01254">
        <property type="entry name" value="PH_PLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09138">
        <property type="entry name" value="PLDc_vPLD1_2_yPLD_like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09141">
        <property type="entry name" value="PLDc_vPLD1_2_yPLD_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06895">
        <property type="entry name" value="PX_PLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000351">
        <property type="entry name" value="Phospholipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1520.10:FF:000055">
        <property type="entry name" value="Phospholipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.870.10:FF:000011">
        <property type="entry name" value="Phospholipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.870.10:FF:000036">
        <property type="entry name" value="Phospholipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.870.10">
        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025202">
        <property type="entry name" value="PLD-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001736">
        <property type="entry name" value="PLipase_D/transphosphatidylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016555">
        <property type="entry name" value="PLipase_D_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015679">
        <property type="entry name" value="PLipase_D_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18896:SF76">
        <property type="entry name" value="PHOSPHOLIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18896">
        <property type="entry name" value="PHOSPHOLIPASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00614">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13091">
        <property type="entry name" value="PLDc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009376">
        <property type="entry name" value="Phospholipase_D_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00155">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56024">
        <property type="entry name" value="Phospholipase D/nuclease"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50035">
        <property type="entry name" value="PLD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 9" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PX" evidence="12">
        <location>
            <begin position="147"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="PLD phosphodiesterase" evidence="11">
        <location>
            <begin position="565"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="domain" description="PLD phosphodiesterase" evidence="11">
        <location>
            <begin position="1083"/>
            <end position="1110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="655"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="712"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="757"/>
            <end position="787"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR009376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010687-PD"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1270" mass="142353" checksum="7789B6820F197313" modified="2023-06-28" version="1">MSLSEIGTSNPALNCDDTGTASSNLTAQLDQQGCHGTDSRGAYYNGTTGGSGDGGAGGGAGAGSAGHHDASLACYSIRSEPEEYDENLDAPDSVTYLSIGGNNPVLVIRESDADSVSPASEIPYSYVYNTPVKFDSMRRHIFIPGLEIIVEIIDYERSLTSHVLNPNLYTVKLTHGPFSWTIQKRYNHFRNLHQQLTTYRASLHIPFPTKSHKERRDSFRNMHTVHQATASPAMQQKLGKPLKKIKKSALPRFPLKPDSLVSFDAIPQRIKQLEEYLYNLLNISLYRNFHGTINFLEVSHISFISALGEKGKEGPIKKRTGSTRPGQSGCNFCGCLAGGCCVRCSYFCTDVLWSKWRNRWFFVKETCFGYYRPKDGVLRCVVLFDQGFDISSGMYSTGMRNGLQIATNSRYLVIKYPTRRIAKEWMTHMKRVANESARDFTLPNPHQSFAPSRPGVQAGWFVDGAGYMSAVADALEGATEEIFITDWMLSPEIYMKRPAIDGDYWRLDKILKRKAEQGIKIFVLLFKELDFALGINSYYSKAKLVEQHENIKVMRHPDHARAGILFWAHHEKLVIIDQTYAFVGGIDLCYGRWDDYQHRLTDLGSISSSTNSSANNTTTRKPSTVVELDENGSVANLLKSSKNIAIATAVDRQAPTSVAVKRADTTQPAAKEPVATNGKQKNPAEKNPEAPGAMGDPPDRVLQAAALALTDQLPLEERDELPENIKQNTPEMERRNIMGMIKDMGRDLKNRITLSEHLDHPQGTDALASGGATGGGSGVKSNVGSPIYLSEEERRKKQLYGGEKGAPLGDLASPQPFKNAAIFELDGQAKLWIGKDYINFIVKDFTNLDSPYADLVDRTTTVRMPWHDVATVVLGQAARDVARHFIERWNAVKLEKCRENANFPYLLPKSYNDIRIDSKFLNVPLHHVTCQVLRSASSWNCGFIEPDYVEQSIHEAYVQTISKAQHYIYIENQFFISMELGNSVVKNQISEYLFKRIVRAHREKKVFRVYVVMPLLPGFEGDVGGSSGISLRAITHWNYASISRGKSSLLERLRAAGIQKPCDYISFHSLRTNSTLNGMPVTELIYVHSKLLIADDKVVICGSANINDRSLLGKRDSEVCVMITDESFEEGRMNGESYPCGVYAGKLRKFLFREHLGLLEPDPKRAPVDVTDPVIHTFWNDVWRRTSRRNTLIYDEVFRCIPSDNVQSFAMMKRFLEDKSLLQSNPEGIQQAVERIEGYLVDLPLKFLCNEILTPPNTSKEGIMPTYMWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9I3BE12</accession>
    <name>A0A9I3BE12_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Cycle</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2023-03" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A9I3BE12"/>
    <dbReference type="EnsemblMetazoa" id="ACON030680-RA">
        <property type="protein sequence ID" value="ACON030680-PA"/>
        <property type="gene ID" value="ACON030680"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041779"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19726">
        <property type="entry name" value="bHLH-PAS_cycle_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050933">
        <property type="entry name" value="Circadian_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001067">
        <property type="entry name" value="Nuc_translocat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013655">
        <property type="entry name" value="PAS_fold_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23042">
        <property type="entry name" value="CIRCADIAN PROTEIN CLOCK/ARNT/BMAL/PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14598">
        <property type="entry name" value="PAS_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08447">
        <property type="entry name" value="PAS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00785">
        <property type="entry name" value="NCTRNSLOCATR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="8">
        <location>
            <begin position="87"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="7">
        <location>
            <begin position="159"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="7">
        <location>
            <begin position="431"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="39"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="354"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="599"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="76"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="369"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="390"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="606"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="621"/>
            <end position="654"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON030680-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="731" mass="78578" checksum="1CD8CD33CF8300D3" modified="2023-06-28" version="1">MTGGPDASLLVDPSLAGTMSAVSNLSTLSAAGSGNHLLHLADHGHSHHDHQHSAGGARKRKFSFNDTSDIEDDTCDDSKSVRTADESKKQNHSEIEKRRRDKMNTYITELSAMIPMCHAMSRKLDKLTVLRMAVQHLKTIRGAVHSYTEGHYKPAFLSDQELKMLILQAAEGFLFVVGCDRGRILYVSESVSHILNYSQGDLLGQSWFDILHPKDVAKVKEQLSSSDLSPRERLIDAKTMLPVKTDVPQGVTRLCPGARRSFFCRMKCKANVQVKEEADQPNSVSSVNNVCHRRKKQVNSDKYSVIQCTGYLKSWAPAKIGLEENETDGEGDSCNLSCLVAVGRVQPNLSQSCSLSSNGLHTPSGANGGRSRNGSNAATGPGSGAGSGATGSSSNGNPSATPSNGSNGKQLNRNTIPNLRNVQFISRHAMDGKFLFVDQRATLVLGFLPQELLGTSMYEYYHHEDIPALAESHKAALQGTQCVTTSVYRLRTKETGFVRLQSEWKSFRNPWTKEIEYLIAKNNVILAELGDGGTARAGGYGMGELGDGTGEPGSGAPGQPGVGYEFFNHTNGREIQRMINSHVEASKIGRQIAEQVLDHQRRVGDSSSESSPNPNEPTLQPAFSSALSEANHSNDAITSGEHSMVTSTGVSPSSMAVVPPTVTTRINGTLPGYSHVQTNAIISPEHDVSQTQASSTDGNDEAAMAVIMSLLEADAGLGGPVDFSGLPWPLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9I3BE50</accession>
    <name>A0A9I3BE50_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Trafficking kinesin-binding protein milt</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2023-03" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the milton family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040237013.2">
        <property type="nucleotide sequence ID" value="XM_040381079.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9I3BE50"/>
    <dbReference type="EnsemblMetazoa" id="ACON030706-RB">
        <property type="protein sequence ID" value="ACON030706-PB"/>
        <property type="gene ID" value="ACON030706"/>
    </dbReference>
    <dbReference type="GeneID" id="120958348"/>
    <dbReference type="CTD" id="45683"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033627"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017022">
        <property type="term" value="F:myosin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048311">
        <property type="term" value="P:mitochondrion distribution"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047496">
        <property type="term" value="P:vesicle transport along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006933">
        <property type="entry name" value="HAP1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051946">
        <property type="entry name" value="Intracell_Traff-Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022154">
        <property type="entry name" value="TRAK1/2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15751">
        <property type="entry name" value="TRAFFICKING KINESIN-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15751:SF12">
        <property type="entry name" value="TRAFFICKING KINESIN-BINDING PROTEIN MILT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04849">
        <property type="entry name" value="HAP1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12448">
        <property type="entry name" value="Milton"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01424">
        <property type="entry name" value="HAP1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01423">
        <property type="entry name" value="Milton"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HAP1 N-terminal" evidence="8">
        <location>
            <begin position="35"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="Trafficking kinesin-binding protein C-terminal" evidence="7">
        <location>
            <begin position="407"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="331"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="698"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="757"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="805"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="934"/>
            <end position="1083"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="125"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="764"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="937"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="974"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="1046"/>
            <end position="1057"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON030706-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1102" mass="118099" checksum="44901BD6CB4267F3" modified="2023-06-28" version="1">MQQASKITEKRDVGCITEVCSAEDLPEVEIFSLLEEQIPRYKIRADTTTTFGGYENQDWFVQYPALPIPTEGLGLTTEQTREALNYFLLCGNRVSQMTRAYDDIDAVTRLLEEKEKDLELTVQIGKELLAQNTHLENRVAELVQELKTTNENRAQLSHELHQKIELIGILTNDADDTSENVTPTASKSINLELLQRKVKQLEDENKSLRTETAQLVKETDECEEQERRLMADIANQLTTANSEFDGLNLELERLKEENRLQHEQIISLTGRLADAEIRLHQLTSENEEASSLLSITKENQNLLAGELAEFKLRYQEVLNLLQEAQEQLRRQRKRSQPLARSSLIPGITGMPAAPDSLQSELMETSLYSEHSLDSGIDSVRGGIGGGGSMMGGMMTGSQQQVPAYRKVFETVRSASRANPNGFSDSFSQLGSMTMSSSSQPRMAPYVYPGLGGTTGNGSGGSNAGITTMTHSYKSGSSVYSTMYGGGGGSSLGGRTYSRESLTADSEDGYPGPAQTGIPGAPGAKDLEAALKRLTPAEVLARRAMLQHAPLGTYSYDEQPAGIPLGCRTPDSIMSTGSSGLSGLSSSSAAGNGQWRLPEKLQIVKPIEGSQTLHHWSRLATPTLSGLLEERPGVTIRGGRGLDELGLQTYSLSDVEEDEDTEDHPGKRFQSFGCTYTYTNSTVLHPDDGTTAVTFSLPPSQMSSQMTSECPTRQPTAPSTPRSSLSRRNSCSTFSVNMGLASMLNERGIKAVTPSALNTPAGPNYSPTVTPCNSPDGSPTRSMSPEPPLLSGLLASTADILRKRFVASTGGQHSSADPADRPSRIMTRNKVALSRLEKKALRSIKIMEKVESIGLENIMLPPQHPPGISPLALHGTSALYTAAASGRGRSPMAQLTSLKHMQDQRRKAQQQQADDLVTIDKETIKAVLTKGLSHDSLKSMASSSGASSGISTAMSSDSDSSSVASFDSEPRTKDTTSGASSAATNTNTASPTTAARLKQMQRQKSRRNLLNGANGGSQRPDLGTVNGSSRPGVRPDLGTVGSKATGKTGGNSSTGGSGKDSRSKQQSTATAAAPAVKEESRSLGQSVYGTISSLLFGRKGGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9I3BE55</accession>
    <name>A0A9I3BE55_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Pyruvate kinase</fullName>
            <ecNumber evidence="17">2.7.1.40</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2023-03" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Pyruvate kinase that catalyzes the conversion of phosphoenolpyruvate to pyruvate with the synthesis of ATP, and which plays a key role in glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>pyruvate + ATP = phosphoenolpyruvate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18157"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.40"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="15">
            <dbReference type="Rhea" id="RHEA:18159"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 17">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 17">Belongs to the pyruvate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.40" evidence="17"/>
    <dbReference type="RefSeq" id="XP_040230484.1">
        <property type="nucleotide sequence ID" value="XM_040374550.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9I3BE55"/>
    <dbReference type="EnsemblMetazoa" id="ACON030709-RA">
        <property type="protein sequence ID" value="ACON030709-PA"/>
        <property type="gene ID" value="ACON030709"/>
    </dbReference>
    <dbReference type="GeneID" id="120954020"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038201"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030955">
        <property type="term" value="F:potassium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004743">
        <property type="term" value="F:pyruvate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00288">
        <property type="entry name" value="Pyruvate_Kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.33.10:FF:000001">
        <property type="entry name" value="Pyruvate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.60:FF:000025">
        <property type="entry name" value="Pyruvate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1380.20:FF:000001">
        <property type="entry name" value="Pyruvate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.33.10">
        <property type="entry name" value="PK beta-barrel domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1380.20">
        <property type="entry name" value="Pyruvate kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001697">
        <property type="entry name" value="Pyr_Knase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011037">
        <property type="entry name" value="Pyrv_Knase-like_insert_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018209">
        <property type="entry name" value="Pyrv_Knase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015793">
        <property type="entry name" value="Pyrv_Knase_brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015795">
        <property type="entry name" value="Pyrv_Knase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036918">
        <property type="entry name" value="Pyrv_Knase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015806">
        <property type="entry name" value="Pyrv_Knase_insert_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004491">
        <property type="entry name" value="PRK05826.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004978">
        <property type="entry name" value="PRK06354.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01064">
        <property type="entry name" value="pyruv_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11817">
        <property type="entry name" value="PYRUVATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PYRUVTKNASE"/>
    </dbReference>
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        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50800">
        <property type="entry name" value="PK beta-barrel domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52935">
        <property type="entry name" value="PK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00110">
        <property type="entry name" value="PYRUVATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13 17" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="9 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 17" id="KW-0460">Magnesium</keyword>
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    <keyword evidence="12" id="KW-0630">Potassium</keyword>
    <keyword evidence="14" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 17" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyruvate kinase barrel" evidence="18">
        <location>
            <begin position="26"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate kinase C-terminal" evidence="19">
        <location>
            <begin position="393"/>
            <end position="510"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001958"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00004997"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008663"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022777"/>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00048967"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00058419"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00224"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF02887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON030709-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="513" mass="55698" checksum="07F3635BE6990EEE" modified="2023-06-28" version="1">MNPEAVSHLEHICSLDIDSKTPFVRLSGIICTIGPASVSPEMLEKMMATGMNIARLNFSHGSHEYHANTIKNIREAVDNYSKKLGKPFPLAIALDTKGPEIRTGLIEGSGTGEVELKKGEKIQLTTDKEHLEKGTKDKIYVDYVNIVKVVKPGDHVFVDDGLISLVVESISGDTLTCTVENGGMLGSRKGVNLPGVPVDLPAVSEKDKSDLAFGVEQGVDVIFASFIRNAAALKEIRTILGEKGKHIKIISKIENQQGMQNLDKIIEATDGIMVARGDLGIEIPAEKVFLAQKSMIARCNRAGKPVICATQMLESMIKKPRPTRAEISDVANAIIDGADCVMLSGETAKGEYPLECVLTMAKTCKEAEAALWHRNLFKDLVDTTPTPLDTAASIAIAGAEASIKSRAAAIIVITTSGRSAHLISKYRPRCPIIAVTRFAQTARQCHLYRGILPVIYEQPAMEDWLKDVDARVQYGIEFGKERGFLKPGNPIVVVTGWKQGSGFTNTIRVINVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9I3BE58</accession>
    <name>A0A9I3BE58_ANOCL</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2023-03" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A9I3BE58"/>
    <dbReference type="EnsemblMetazoa" id="ACON030710-RC">
        <property type="protein sequence ID" value="ACON030710-PC"/>
        <property type="gene ID" value="ACON030710"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_036950"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="443"/>
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    </feature>
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            <end position="627"/>
        </location>
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            <end position="841"/>
        </location>
    </feature>
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        <location>
            <begin position="856"/>
            <end position="892"/>
        </location>
    </feature>
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        <location>
            <begin position="968"/>
            <end position="1025"/>
        </location>
    </feature>
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        <location>
            <begin position="87"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
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            <begin position="127"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="180"/>
            <end position="197"/>
        </location>
    </feature>
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            <begin position="216"/>
            <end position="228"/>
        </location>
    </feature>
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            <end position="269"/>
        </location>
    </feature>
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        <location>
            <begin position="330"/>
            <end position="340"/>
        </location>
    </feature>
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        <location>
            <begin position="349"/>
            <end position="362"/>
        </location>
    </feature>
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            <begin position="383"/>
            <end position="392"/>
        </location>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    <sequence length="1025" mass="109301" checksum="AB0AB7E2B9F03301" modified="2023-06-28" version="1">MDRSSYGGPPQSTIGNSARRIQAQLPAEPPACVQNAMMRRDKQPFTYTPGGIDLSQIKSPRMAKRISRNANSEGVTGQPKVSPLAQNNGSSYSSNGTSSSNGNTSSVSPAATLGAAAMGMPFQVFPTGPPAPPPPPPPTGRVLPSNGGNRSIPPPPPPPGPAQPTASSSDARKSPRPQSFEPPPMGMRPEIKIPPNPMAALRKAPRPQPKNDFWIEEYRREKQQDERPQQIPRPVPPSYSYTNRDLDDEVAKNTFNRTDELTVRGDHPLAQHHHRSPSPSNLTVPHESGSRGSSSIKTPPVYGQSPVPANLPPPITPTKVSAHPPATKDSPPPPPPPVRPKPSLSPVSTSNQYSGSTTSTPLHQPGGGSAGKEQTAGVKAAAAPPPPPPPPHASSASSSHKDTAQSSGVSPSVSSASSSGSPNQSPTSAKPTSTQRPAAQALGSLYIPPIHEVFANSKQSLLTQASPPWMSSRHNSLKEQPEWVHKDEQDSTTNTNATQSPEQSVEQEQSSKPPASSTSGSPRAPSVAKESVAATSKPVDSIVTAPTAVSARQAVPSQPLTPNSNRSAAPAADVSNPVQHQQTGTASGQQQQQQYGSQQQQQQQQTGRATGGLPAAGTVSSTVTMPVNRGYPQQAPVYAQAQPVYSTQSPAGTQKERIIPIQIEKSPVQAPVTAPTFAPPPYYSPSAQYGPASAQSSMQSPIAVGFATPHSGFTTPTAMFTNPNHFVNQGYNNISYPPTPTGQPHPHYQHHQHYAPPQTPPMHHPMHPQAQQQRMQQSTPTQQGQQVTNVRIVPIKVESGSDHTTVRGPLSNTPAIIQSDPRSSSNAQSWNGNNAPNQSRSFRVLQQITDTLDEAEAEKNAKEQSNNQSDQADGAKQQYSRSTAQQQDAAEGQLRRLQLSNEDKALMNRVKNQVDGEVYFHNEEDPRYRGAAIPSKAFRYLQNMTDSGQAANQSNNGSGNRAQQMFNKANYNDSDGDTQQSQQYVPPSEQKVEEPKKYTGGSIPSRSFKMLQAMTDAPGPGETDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABM2A8E3</accession>
    <name>A0ABM2A8E3_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12 13">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON000159.R230">
        <property type="protein sequence ID" value="ACON000159.P230"/>
        <property type="gene ID" value="ACON000159"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd01367">
        <property type="entry name" value="KISc_KIF2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054473">
        <property type="entry name" value="KIF2A-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47971:SF8">
        <property type="entry name" value="KINESIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47971">
        <property type="entry name" value="KINESIN-RELATED PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22923">
        <property type="entry name" value="KIF2A-like_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4 13" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="9 12 13" id="KW-0505">Motor protein</keyword>
    <keyword evidence="5 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="15">
        <location>
            <begin position="317"/>
            <end position="648"/>
        </location>
    </feature>
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        <location>
            <begin position="65"/>
            <end position="118"/>
        </location>
    </feature>
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            <begin position="133"/>
            <end position="214"/>
        </location>
    </feature>
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        <location>
            <begin position="90"/>
            <end position="102"/>
        </location>
    </feature>
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        <location>
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            <end position="189"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="407"/>
            <end position="414"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000159.P230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="792" mass="89052" checksum="502DB1739236F691" modified="2025-10-08" version="1">MEMFEKGSVIYITRSDGRIHTAIVSRFHEDTRSVTVEWYERGESKGKEIEIDMLLELNRTMQLQPQLQQELHHQQQHQHQQHYQQQQQQHEPEPKPTAEEAKQRKKTPAPANLARNPTQAIIRYNNYLAQRGDSGKGASIPAPSNFNRATILHTGPTQRSEQSGTGRTTNVSSRILGNSNNTGRSTMINNRAAEQPAEIRTQPKALGKTSVATGQQQTTAPAAAAAAAAPASAVANANNAVANSIPRKSSCVVTVGMMEENRIKQRDMFKVMREKKNALMNQDGGNPNWEFINMIREYQSTIDFRPLVQGQSIDQHQITVCVRKRPLNQKEQTRKEVDVVCIPNKDTVIVHEPKAKVDLTKYLDNQKFRFDCTFDDTCSNEMVYMYTAKPLVQAVFEGAMATCFAYGQTGSGKTHTMGGTFNGKSQDSMNGIYALATRDIFELLQSPKYQSNNLVVSASFYEIYCGNVYDLLANKNKARVLEDGKKQVQVVGLTEREVNSVDEVLAIISSGSNLRTSGQTAANSNSSRSHAIFSLTLRVQASPKVHGKFSFIDLAGNERGADTVSENRRTRSESSEINKSLLSLKECIRALGRKRHLPYRGSVLTKVLRDSFVGKNIRTCMIAMIAPGMASCEHTLNTLRYAHRVKELAVVDPDDRVDPEEDLIECDEPSNGGTHNFNDLEQLRSLNEQEMTMELYNQHKAISDLQQKEEEVLDNHQRVNEFLEKFLPEAKELYNFTNLVDYDQDAYCKRGEELFSQLAEVANAFKDLMSEFRTKLSQEEIVSQAAKLSHRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABM2A8F3</accession>
    <name>A0ABM2A8F3_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cadherin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_040224247.2">
        <property type="nucleotide sequence ID" value="XM_040368313.2"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON000153.R228">
        <property type="protein sequence ID" value="ACON000153.P228"/>
        <property type="gene ID" value="ACON000153"/>
    </dbReference>
    <dbReference type="GeneID" id="120950297"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24026:SF96">
        <property type="entry name" value="CADHERIN-86C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24026">
        <property type="entry name" value="FAT ATYPICAL CADHERIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Cadherin" evidence="10">
        <location>
            <begin position="204"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="10">
        <location>
            <begin position="306"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="10">
        <location>
            <begin position="423"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="10">
        <location>
            <begin position="540"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="713"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="931"/>
            <end position="950"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="978"/>
            <end position="1051"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1172"/>
            <end position="1192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1260"/>
            <end position="1359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1376"/>
            <end position="1475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1518"/>
            <end position="1780"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1796"/>
            <end position="1826"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="1202"/>
            <end position="1236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="716"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="933"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="978"/>
            <end position="987"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="988"/>
            <end position="1005"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1017"/>
            <end position="1036"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="1268"/>
            <end position="1286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="1321"/>
            <end position="1336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="1337"/>
            <end position="1353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1383"/>
            <end position="1394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1416"/>
            <end position="1438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="1455"/>
            <end position="1465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1518"/>
            <end position="1531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1535"/>
            <end position="1544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1545"/>
            <end position="1554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="1601"/>
            <end position="1615"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1632"/>
            <end position="1649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1651"/>
            <end position="1671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1691"/>
            <end position="1714"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1720"/>
            <end position="1736"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1796"/>
            <end position="1808"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="1809"/>
            <end position="1818"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000153.P228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1883" mass="205039" checksum="A794EDBDDB07A5EA" modified="2025-10-08" version="1">MTEQRPHRLARYVHCRSIVPSSINQSHIKMTRNGVTCAGAYSSTSGRRKRPDHTSPRIPLTLALLALAACWPEAAGLDPKFDPSTRMGLVLVPADATVGSVIYRLRASDEEFEYPLQFELVGDASSSTVQIETLPCTKFNSICQANVILTRRLEPGRYYDFQVSVKDTKGGMSVQSCSITATNFTTPHDVIFPHKAGIIMVPEDAKKGTELDYVLANKNPLFQKPVYLELWGSPLFGIRQKFISPETAEGTIFLLGQLDFEKQAMYHLTVLANDAYAEPGQDTRNIAGLEVVIIVEDVQDVPPVFTVAPPVTRLPAGLIPGDKVLQVHAEDGDKGVPREIRYGLVSEGNPFTSFFDINETSGEISLLRPLDDILALTHAGDPVLLTVIAEEVKVSRDEPPAMATTVQLAFFLPERSNSPPYFENDHYVARLDENAAPGTVLTFTDPYTPRVMDDDAGKNGVFSLTLLGNNGTFEISPNVAEGHANFVVRVRDSARLDYEAATYVHFQILAQELGPATNLSMLVNVTVYLADVNDNPPVFEQADYIVDLPENMTAGTRVVQVHATDVDTARLGGRVRYTQILGPHNTSLNLDPASGVVTVALNNHGFDREAMPEYHLYVEARDDDGIGNRAQVPLVIRLIDVNDETPAFEKPLYEFILAADLRNFTVPAFIRATDGDAEAPNNEVRYELIYGNYENKFFLHPITGELKLNGPLVPRTGGGGGGGQSVGGLRRRRQTGGSVASGGPPATGQKESDVFVLTARAFDLGVPVRYSTATIRVYPPESRTRTVTFIVPGSDPDRKKVQDTLADITGGRVIIQEVRPYRTGDGGIPTDLIGGGGGGGSGGGAERSVVIATILYDADSVVDIAKIQQRLSINGTVTNIISQEERSAILYKAENRVLFWLLIFLAILLALGILTLLLCCICPRCPLNATNRSDMKSKSTKDDTHMHRSRTNLLNADRDMYVEDVDEHDPEYQSLKRIHHHHHHHHAGGGGGGGGGGGGGGGGGKPTTDDALPIDDDSMRRHEMDRGSDLNYERRGSFKRQGHAAAPQLVADDIEPRDQYFIKDGNAEILRLITRGRNEEENIYVNIPQQQQAQQQQQRAGVAAQPNQPQYIMVDNGGKEILMRRYIEEQANGKQIIREHYQLLPGTTFIQTIPNEVPVRRGEHVTEVKIGAGAGRSKDPAADGDGDPGRLKPTVSTHSLMHQELEHSLKQQNALLRQILLEKEKLEERYNQYEQALETQSLPCQSMAVVVAATQTDCDTGTQTEPMRGGGGGGGGAGGFGASGGFGRRRTKSENDDSLSEDEYEYVRYSPPDSPDGVYLIKRRRHRKKTLKHRTGGKGGLGGGSGEYSGGESTGERKSNRRIIVVESVKRKIRTPIQEEEDELHHGRELRREGPGGGGHRRGGQETRTSILRRKRNEELSRNGKEPGKEQRLKRDVLMEISDSLHGPEQRGRRGGGNGGNGAPGADGRRKKVYRKNVKYYEDSDGSEKEVVVQKNYFSADSLDEMASDVEDYRYSVTKTSKSVTVSPKASVEQRVSRRDDKTETTTTRIRLTTSESPSRRQAAGTGGSGAMAGSQGPKGPAPKPPRLSKSVSKEEDLNEPGGGGGGGGGGGEGQQHGVNPKYMDWYYNLSKEGDSLEKRDGRKRDREPAGGTTLLTTTTTSSTTTITTGGSRRKGEGEPAAAAPAPPAKGKPEPAPRTSPPKEARLLKEDIQHARKVAQSQQQQQQQQQQQQQQQEAGGSHPLLQHSEHRFEAEYGTGPPVVPAPPDKLPHYLYPETPPIVSRDNKVQIKIVDQSAAATAAPGAKSNGAGGGGGGGTSTKNAKTLNVSTLEDDHDSGIAMNSLLHTKGKRNKIADKKSIFTIAYDDVRIRQIRSESDTPPFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABM2AA70</accession>
    <name>A0ABM2AA70_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Myosin motor domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040240874.1">
        <property type="nucleotide sequence ID" value="XM_040384940.2"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON000776.R208">
        <property type="protein sequence ID" value="ACON000776.P208"/>
        <property type="gene ID" value="ACON000776"/>
    </dbReference>
    <dbReference type="GeneID" id="120961111"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd21759">
        <property type="entry name" value="CBD_MYO6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01382">
        <property type="entry name" value="MYSc_Myo6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21958">
        <property type="entry name" value="MyUb_Myo6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22249">
        <property type="entry name" value="UDM1_RNF168_RNF169-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1590">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.220.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.360">
        <property type="entry name" value="Myosin S1 fragment, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049016">
        <property type="entry name" value="MYO6_lever"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032412">
        <property type="entry name" value="Myosin-VI_CBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008989">
        <property type="entry name" value="Myosin_S1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036114">
        <property type="entry name" value="MYSc_Myo6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140">
        <property type="entry name" value="MYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140:SF745">
        <property type="entry name" value="UNCONVENTIONAL MYOSIN-VI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21521">
        <property type="entry name" value="MYO6_lever"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16521">
        <property type="entry name" value="Myosin-VI_CBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="5 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 10" id="KW-0505">Motor protein</keyword>
    <keyword evidence="7 10" id="KW-0518">Myosin</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myosin motor" evidence="13">
        <location>
            <begin position="56"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="10">
        <location>
            <begin position="648"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="938"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1098"/>
            <end position="1125"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="840"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1098"/>
            <end position="1107"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="150"/>
            <end position="157"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000776.P208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1243" mass="142052" checksum="F43A8E1AA1393985" modified="2025-10-08" version="1">MLDSQLVWARDIQDGYIQGRITEIGASEYDVQPVDSKLPKRSCIPEDIFPSCERPSDNDDNCELMFLNEATLLDNIRNRYYKDKIYTYVANILIAVNPYKEIPDLYSTATLKRYSGKSIGELPPHVYAIADKAIRDMRVLKMSQSIIVSGESGAGKTESTKYLLKFLCDSVAAAGPIEQKILDANPILEAFGNAKTTRNNNSSRFGKFIEVHYDKRCQVVGGHISHYLLEKSRICTQSPDERNYHVFYLLCAGAPPALRERLALTKPDDYRYLCGCTQYFTHPHTESRIPAGHKSRVHAAKGPLKDPILDDYEDFRQLDEALGRLGLSEAERTQIYSLVAAVLHLGNIRFEENPEDSNGGCRITQESQPSLNVTARLIGVDPSELRQALMSRVMKSKGGGIKGTVIMVMLKVYEANNARDALAKALYSKLFDHIVSLINQNIPFQASSYYIGVLDIAGFEYFTVNSFEQFCINYCNEKLQKFFDDNILAAEQVLYAREGLNVPEIKYTDNQDIIELIESKSNGIFTLLDEESKLPRPSYAHFTNEVHSAWNGHYRLSFPRASRLKAHRSLRDDEGFLVRHFAGAVCYNTNQFIEKNNDALHASLEGLVQESENALLNALFSGGGAGKSPMNVGKLSFISVGSKFRSQLGELLEKLRNNGTNFIRCIKPNSRMIDHEFEGSLALAQLKYSGTNSVLELMEYGYPSRVPFNELYNMYKSYLPPELARLAPRTFCEAMLMSLKLNSNEFKFGITKVFFRPGKFVEFDRIMRSDPENLQAIVAKVKKWLIRSRWIKSAFAAICVIKLKNRIIFRNKSVVLLQKTIRGYLVRKKHQPRYRGIAKIKAVEDNLRKMHAIAEQLKADRDKMQANIRDVQASLTAAVAKIRANPSIGAAAIDQQYADIMRSIDTNMKLINARLQEQRNAEEQERLNKIKLALEAERRAKEEEERRLREEEENRKRKTEIEARRKEEEAKRLRQEGEDRKAALALQAQLEKEAQEDIQYRQQLEQERRDHELALRLAQESNGQVEDSPPALRKSEAIRAQQQLVEKQKHDLSKWKYSELRDAINTSCDIELLEACRHEFHRRLKVYHAWKAKNRKRTTMDENERAPRSVMEAAARSPPRLTPKQEIVPTGSVHRYFRIPFVRPSNGGGPAEIGTAAANADDSKRSWWFAHFDGQYVARQMELHTDKPPILLVAGKDDMQMCELSLEETALTRKRGAEILEHEFNREWEQHGGKPYNRLSARK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABM2AXD1</accession>
    <name>A0ABM2AXD1_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_040234452.2">
        <property type="nucleotide sequence ID" value="XM_040378518.2"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON008318.R248">
        <property type="protein sequence ID" value="ACON008318.P248"/>
        <property type="gene ID" value="ACON008318"/>
    </dbReference>
    <dbReference type="GeneID" id="120956788"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051291">
        <property type="entry name" value="CIMAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010736">
        <property type="entry name" value="SHIPPO-rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21580:SF28">
        <property type="entry name" value="BOREALIN N-TERMINAL DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21580">
        <property type="entry name" value="SHIPPO-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07004">
        <property type="entry name" value="SHIPPO-rpt"/>
        <property type="match status" value="31"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="122"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="393"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="587"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1118"/>
            <end position="1144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1118"/>
            <end position="1142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008318.P248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1211" mass="128881" checksum="DC891202C84C901A" modified="2025-10-08" version="1">MGGFEQRPWTPTKRRGPIAAEHRGPGPQYLLPQLLGTKVVDSKRQAAPAYSFGQRHKPRTESFSPGPLYNITGLGCKGKDAPPAYCLQSRPKEIPKYLTPAPGEYNIEKADKMLVKSAPKYTFGVKKPPNPTSQTPAPGDYKPERVALDSTPKFSFGIKTESKIRNDTPAPGTYSPEKVLKDKSPKYSFGLKVIPEKIEETPAPGAYQPEKALALDKKPAYSFGLKTIVEKPNNTPAPGAYEPEKVKLNATPAYSFGIRPAIEKPNSNPAPGAYEPEKAKKLIDHSPAFAFGMRINHDKPNNTPGPSAYQVEKVNLDHQPAYSFGMRTKVAKPNVTPAPGAYCPEKVNLDSTPKYSFGIRPTIDKPDNVPAPGAYSPEKAKVDSSPAYSFGVRAKQEKPSANPGPGAYDADKVDGKSPAYSFGLRPDVAKPNQVPGPGAYNADRVDHATPAYSFGVKANVQKPNLVPGPGAYDADKVHDKSSPAYSFGVKGNAEKPNAIPGPGAYDADKVIDKSAPAYSFGARTNVDKPSVVPGPGAYDSDKVNDKSSPAYSFGVKTNLEKPNAIPGPGAYDADKVHDKSTPAYSFGVKHMPDKPNAVPGPGAYDSDKANDKSSPAYSFGVKTNLEKPSAIPGPGAYDADKVIDKSSPAYSFGVKANVEKPNTIPGPGAYDADKVNDKTTPAYSFGVKPNLEKPNSIPGPGAYDADKVIDKSSPAYSFGVKTNVEKPNAIPGPGAYDADKVHDKSTPAYSFGVKPNLEKPNAIPGPGAYDADKVHDKSSPAYSFGVKTNVEKPSIIPGPGAYDADKVIDKSSPAYSFGVKTNVEKPSIIPGPGAYDADKVIDKSSPAYSFGVKHSLEKPHIVPGPGAYDADKVIDKTSCPAYSFGIKTNVEKPSIIPGPGAYDTEKVHDSHTPAYTFGVKPQTTKVNPTPAPGAYSPEKYIIHHPAYTFGVKPHIDKIDIIPAPGAYNPEAVKLDSSPAYTFGVKPVIDKVDMTPAPGTYSVEKVKLDHSPAYPFGIKVNREKPNDVPAPGSYNPDKVKLDHSPAYTFGIKHKGETMRDTPAPSAYQPEKCKNDCSPAYSFGVKVNHESIPHDGPVVTSDEATAVAAMKAMVITNGASSTVDNRSESKNATTSSTVVQNGQDGSTFTTVTTTRTSGGSMKMVRERKTTTEEHSSASVEAIGEGLVPNCIAGVKQLANVQHQRMVCTGHFRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A182LB79</accession>
    <name>A0A182LB79_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Calcium release-activated calcium channel protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Orai family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A182LB79"/>
    <dbReference type="STRING" id="1518534.A0A182LB79"/>
    <dbReference type="EnsemblMetazoa" id="ACOM035072-RA">
        <property type="protein sequence ID" value="ACOM035072-PA"/>
        <property type="gene ID" value="ACOM035072"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON005071-RA">
        <property type="protein sequence ID" value="ACON005071-PA"/>
        <property type="gene ID" value="ACON005071"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000841"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACOM035072"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005071"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038113"/>
    <dbReference type="Proteomes" id="UP000075882">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015279">
        <property type="term" value="F:store-operated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002115">
        <property type="term" value="P:store-operated calcium entry"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.140:FF:000002">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.140">
        <property type="entry name" value="Calcium release-activated calcium channel protein Orai"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012446">
        <property type="entry name" value="CRAC_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038350">
        <property type="entry name" value="Orai_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31501">
        <property type="entry name" value="CALCIUM RELEASE-ACTIVATED CALCIUM CHANNEL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31501:SF7">
        <property type="entry name" value="CALCIUM RELEASE-ACTIVATED CALCIUM CHANNEL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07856">
        <property type="entry name" value="Orai-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="177"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="235"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="266"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="30"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005071-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="337" mass="36575" checksum="37AB89FA50139D64" modified="2016-09-07" version="1">MHLHPSCSQSNLCLNSPLYRNFNHPATATPATALRVSPQPVPKVRTSRSHSSSSALGGGGGFGGASGVGDFWTGGSAHPNSCSVNTGLLSGLGAAGASFHGGAASSLFAQEAFRRSNLSTMSQTGDDLHTPNYLSWRKLQLSRAKLKASSKTSALLSGFAMVAMVEVQLDENTKVPSAMLIAFAVCTTLLVAVHMLALMISTCILPNIETVCNLHSISLVHESPHERLHWYIETAWAFSTLLGLILFLLEIAILCWVKFYDLNTTAAWSACIVLIPVLVIFVAFALHFYRSLMMHKYEVTVSGIRELEILKEQMEQDHFDQQQHHHGILHPSGLQIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A182LJE0</accession>
    <name>A0A182LJE0_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="4">MSP domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A182LJE0"/>
    <dbReference type="STRING" id="1518534.A0A182LJE0"/>
    <dbReference type="EnsemblMetazoa" id="ACON005556-RA">
        <property type="protein sequence ID" value="ACON005556-PA"/>
        <property type="gene ID" value="ACON005556"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_003590"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACOM038999"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005556"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037101"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140284">
        <property type="term" value="C:endoplasmic reticulum-endosome membrane contact site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053012">
        <property type="entry name" value="ER-organelle_contact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000535">
        <property type="entry name" value="MSP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008962">
        <property type="entry name" value="PapD-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46384">
        <property type="entry name" value="MOTILE SPERM DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46384:SF1">
        <property type="entry name" value="MOTILE SPERM DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00635">
        <property type="entry name" value="Motile_Sperm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49354">
        <property type="entry name" value="PapD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50202">
        <property type="entry name" value="MSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="227"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="MSP" evidence="3">
        <location>
            <begin position="33"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005556-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="265" mass="29314" checksum="31979B725983B13F" modified="2023-06-28" version="2">MARVHFANLSPPESPMLDKPSTTFQPESSEGEMLRIKPQNTITFSRLGNDLVGTVEITNVDTKDVSYKVKTTAPEKFRVRPSTGVLPPSASVNINVMLQQGQQMHTINREKFLVMCIGLTDEISTNPQDLTELWKKISAKSSSVEQHRLKCALPANCDESGLESMFGGAGNVIAGPDQYGVMSGSFGGSSMMGMGADKQMSLLQQTISHLNETTHRLEVQVKRNHRFQWITCVLFVLLALAIVYILKIEIKSHASEYCTTAERNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>A0A182LJE3</accession>
    <name>A0A182LJE3_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Splicing factor 3B subunit 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the RSE1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040220774.1">
        <property type="nucleotide sequence ID" value="XM_040364840.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A182LJE3"/>
    <dbReference type="STRING" id="1518534.A0A182LJE3"/>
    <dbReference type="EnsemblMetazoa" id="ACON005549-RA">
        <property type="protein sequence ID" value="ACON005549-PA"/>
        <property type="gene ID" value="ACON005549"/>
    </dbReference>
    <dbReference type="GeneID" id="120948473"/>
    <dbReference type="CTD" id="23450"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_014006"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACOM039002"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005549"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041533"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001143">
        <property type="entry name" value="Pre-mRNA-splicing factor rse-1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.910:FF:000002">
        <property type="entry name" value="Splicing factor 3B subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000031">
        <property type="entry name" value="Splicing factor 3b subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018846">
        <property type="entry name" value="Beta-prop_RSE1/DDB1/CPSF1_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058543">
        <property type="entry name" value="Beta-prop_RSE1/DDB1/CPSF1_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050358">
        <property type="entry name" value="RSE1/DDB1/CFT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004871">
        <property type="entry name" value="RSE1/DDB1/CPSF1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10644">
        <property type="entry name" value="DNA REPAIR/RNA PROCESSING CPSF FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10433">
        <property type="entry name" value="Beta-prop_RSE1_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23726">
        <property type="entry name" value="Beta-prop_RSE1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03178">
        <property type="entry name" value="CPSF_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101908">
        <property type="entry name" value="Putative isomerase YbhE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="RSE1/DDB1/CPSF1 first beta-propeller" evidence="8">
        <location>
            <begin position="1"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="RSE1/DDB1/CPSF1 second beta-propeller" evidence="9">
        <location>
            <begin position="315"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="domain" description="RSE1/DDB1/CPSF1 C-terminal" evidence="7">
        <location>
            <begin position="734"/>
            <end position="1053"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF10433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005549-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1087" mass="120986" checksum="DF4B5B6B75EB2FFF" modified="2023-06-28" version="2">MIGAVEKQKLVYILNRDSEARLTISSPLEAHKSNTLTYHMVGVDVGFENPMFACLEIDYEEADTDPTGEAATKTQQTLTFYELDLGLNHVVRKYSEPLEEHANFLISVPGGNDGPSGVLICSENYLTYKNLGDQHDIRCPIPRRRNDLDDPERGMIFICSATHRTKSMYFFLVQTEQGDIFKVTLETDDDVVSEIKLKYFDTVPPATAMCVLKTGFLFVACEFGNHYLYQIAHLGDDDDEPEFSSAMPLEEGDTFFFAPRQLKNLVMVDDIPSYAPILGCQVADLANEDTPQLYLACGRGPRSSIRVLRHGLEVSEMAVSELPGNPNAVWTVKKRIDDEFDAYIIVSFVNATLVLSIGDTVEEVTDSGFLGTTPTLCCSALGDDALVQVYPDGIRHIRADKRVNEWKAPGKKTIMKCAVNQRQVVIALSGGELVYFEMDPTGQLNEYTERKKMPSEVMCMALGSVPSGEQRSWFLAVGLADNTVRIISLDPTDCLSPRSMQALPSAAESLCIVEMGTVETSSEDDGVTITTGCIYLNIGLTNGVLLRTVLDPVSGDLADTRTRYLGSRPVKLFRIQMQGSEAVLAMSSRSWLSYYYQNRFHLTPLSYETLEYASGFSSEQCSEGIVAISTNTLRILALEKLGAVFNQITFPLEYTPKRFAIHQETGKLIISETDHNAYTEETKTVRKKQMADEMREAAGEDEQELANEMADAFINEVLPEDVFSSPKAGTGMWASQIRVMDPINGHTYSKVQLAQNEAVLSLALVRFAVDQKWYVVAGVAKDLQINPKISGGGFIDVYKVDSQTHQLEHMHRTEIDDAPGALCPFQGRLLAGIGKVLRIYDLGKKKLLRKCENKHIPNQIVNIQGMGQRVYVSDVQESVYCIKYKRAENQLIIFADDTHPRWITSASLLDYDTVATGDKFGNIAILRLPHSVSDDVDEDPTGNKALWDRGLLNGASQKAENICTFHLGEIVMSLQKATLIPGGSESLIYATMSGTVGALVPFTSREDYDFFQHLEMHMRNENPPLCGRDHLSYRSYYYPVKNVMDGDLCEQFTSLDPAKQKSIASDLGRTPSEVAKKLEDIRTRYAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-09-07" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A182LNJ9</accession>
    <name>A0A182LNJ9_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glutamate dehydrogenase [NAD(P)(+)]</fullName>
            <ecNumber evidence="3">1.4.1.3</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-glutamate + NAD(+) + H2O = 2-oxoglutarate + NH4(+) + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15133"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.4.1.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-glutamate + NADP(+) + H2O = 2-oxoglutarate + NH4(+) + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11612"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.4.1.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Glu/Leu/Phe/Val dehydrogenases family.</text>
    </comment>
    <dbReference type="EC" id="1.4.1.3" evidence="3"/>
    <dbReference type="RefSeq" id="XP_049462673.1">
        <property type="nucleotide sequence ID" value="XM_049606716.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A182LNJ9"/>
    <dbReference type="STRING" id="1518534.A0A182LNJ9"/>
    <dbReference type="EnsemblMetazoa" id="ACOM041006-RA">
        <property type="protein sequence ID" value="ACOM041006-PA.1"/>
        <property type="gene ID" value="ACOM041006"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON004362-RB">
        <property type="protein sequence ID" value="ACON004362-PB"/>
        <property type="gene ID" value="ACON004362"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON004362-RC">
        <property type="protein sequence ID" value="ACON004362-PC"/>
        <property type="gene ID" value="ACON004362"/>
    </dbReference>
    <dbReference type="GeneID" id="120961082"/>
    <dbReference type="CTD" id="42832"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004833"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACOM041006"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004362"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040704"/>
    <dbReference type="Proteomes" id="UP000075882">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004352">
        <property type="term" value="F:glutamate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006538">
        <property type="term" value="P:L-glutamate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01076">
        <property type="entry name" value="NAD_bind_1_Glu_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10860:FF:000007">
        <property type="entry name" value="Glutamate dehydrogenase 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000100">
        <property type="entry name" value="Glutamate dehydrogenase 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006095">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006096">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006097">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033524">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033922">
        <property type="entry name" value="NAD_bind_Glu_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11606">
        <property type="entry name" value="GLUTAMATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11606:SF13">
        <property type="entry name" value="GLUTAMATE DEHYDROGENASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00208">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02812">
        <property type="entry name" value="ELFV_dehydrog_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00082">
        <property type="entry name" value="GLFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00839">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00074">
        <property type="entry name" value="GLFV_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamate/phenylalanine/leucine/valine/L-tryptophan dehydrogenase C-terminal" evidence="9">
        <location>
            <begin position="261"/>
            <end position="551"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004362-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="554" mass="61332" checksum="0B8468EE5977551A" modified="2016-09-07" version="1">MYHLKTIAKNATIKQEVATLVKAVAAVQQPMAMNQQQPKRTYAEHQIPERLKDVSTAANPRFFDMVEYFFHRACQICEDKLVEDMKGRASMDEKKKKVKGILMLMQPCDHIIEIAFPLRRDSGDYEMITGYRAQHCTHRTPTKGGIRFSLDVSRDEVKALSALMTFKCACVDVPFGGAKAGVKIDPRKYSEHELEKITRRFALELSKKGFIGPGIDVPAPDMGTGEREMSWIADTYAKTIGHLDINAHACVTGKPINQGGIHGRVSATGRGVFHGLDNFIKEANYMAQIGTTPGWGGKTFIVQGFGNVGLHSCRYLTRAGATCIGVIEHDGSIFNPQGIDPKALEDYKNEKGTIVGFPGAQPYEGENLMYEQCDIFIPAAVEKVITAENAGKINAKIIAEAANGPTTPAADKILIDRNILVIPDLYINAGGVTVSFFEWLKNLNHVSYGRLTFKYERESNYHLLESVQRSLEARFGTVGGKIPIEASEAFKKRISGASEKDIVHSGLDYTMERSARAIMITAMRYNLGLDLRSAAYINSIEKIFQTYRDAGLAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V0T5</accession>
    <name>A0A6E8V0T5_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">SNF-related serine/threonine-protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">SNF1-related kinase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="23">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">May play a role in hematopoietic cell proliferation or differentiation. Potential mediator of neuronal apoptosis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8V0T5"/>
    <dbReference type="EnsemblMetazoa" id="ACON001752-RB">
        <property type="protein sequence ID" value="ACON001752-PB"/>
        <property type="gene ID" value="ACON001752"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_003442"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001752"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037759"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14074">
        <property type="entry name" value="STKc_SNRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14339">
        <property type="entry name" value="UBA_SNRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000003">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000166">
        <property type="entry name" value="SNF-related serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF45">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 20 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="10 20 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 21" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="22">
        <location>
            <begin position="25"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00054738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00074971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00077142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001752-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="378" mass="42830" checksum="E3E8ADDBFDB7369E" modified="2020-08-12" version="1">MQRSFGGLSAASRAGVYDGKIAGLYDLEETLGSGHFAVVKLARHVFTGEKVAVKVIEKTKLDEISRAHLFQEVRCMKLVQHPNVVRLYEVIDTQTKLYLILELGDGGDLYDYIMRHEKGLSENVAREYFRQIVRAISYCHQLHVVHRDLKPENVVFFEKLGVVKLTDFGFSNKFCPGQKLETSCGSLAYSAPEILLGDSYDAPAVDVWSLGVILFMLVCGHPPFQEANDSETLTMIMDCKYTMPAHVSDGCRNLISKMLVREPEKRATLQQIALDVWLMEGSCEETPENQPLVSREQVSEEDHTLIIQKMINGKIATKEEILEALDRNEYNHITATYFLLAERKLRAHRQEEAQKRKPELTLPVSNSTRNLTFLGLHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V344</accession>
    <name>A0A6E8V344_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Facilitated trehalose transporter Tret1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">High-capacity facilitative transporter for trehalose. Does not transport maltose, sucrose or lactose. Mediates the bidirectional transfer of trehalose. Responsible for the transport of trehalose synthesized in the fat body and the incorporation of trehalose into other tissues that require a carbon source, thereby regulating trehalose levels in the hemolymph.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Trehalose transporter subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040239419.1">
        <property type="nucleotide sequence ID" value="XM_040383485.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8V344"/>
    <dbReference type="EnsemblMetazoa" id="ACON001027-RA">
        <property type="protein sequence ID" value="ACON001027-PA"/>
        <property type="gene ID" value="ACON001027"/>
    </dbReference>
    <dbReference type="GeneID" id="120959815"/>
    <dbReference type="CTD" id="39458"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009687"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001027"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040419"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051119">
        <property type="term" value="F:sugar transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015574">
        <property type="term" value="F:trehalose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17358">
        <property type="entry name" value="MFS_GLUT6_8_Class3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000055">
        <property type="entry name" value="Facilitated trehalose transporter Tret1-2 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044775">
        <property type="entry name" value="MFS_ERD6/Tret1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050549">
        <property type="entry name" value="MFS_Trehalose_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48021">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48021:SF1">
        <property type="entry name" value="GH07001P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="73"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="103"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="128"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="161"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="186"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="271"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="309"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="335"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="369"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="405"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="438"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="15">
        <location>
            <begin position="34"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="479"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="487"/>
            <end position="500"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00060205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00069106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001027-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="500" mass="53842" checksum="CD0F7C1EF2755A78" modified="2020-08-12" version="1">MVQTVLKRARDAMSKFVVNNVAGESGRKLPQYIAGLAASGGALAAGTFLGWTAPTDKPLTVEGEYGFPVSDEQFSWVGSISNLGAALMCFPIGYMMKLIGRKWSMLAMVLPLVLGWLLIIFAENVAMLMVGRFFLGVGGGAFCVAAPTYTAEIAQPSIRGTLGTFFQLMVTVGILFVYAVGAGVDVQVLSIICGVIPLVFGAIFFFMPESPYYFVEKGRYSEAASSLKWLRGAQYDENAEIEDLKQADEKVKAEAIPMLVAFRQKATVRALAISLGLMFFQQLSGINAVIFYNSAIFASANGGKEMSSASIIVGGIQVVATLLASVVVDKVGRRILLLVSDLMMAVSTILLAVYFQLKQDDPAKVDDLSWLAVLAVCLFIAMFSIGYGPVPWLMVGELFANNVKAFASPVAGVFNWLLAFLVTKVFTNLKDAMGEAGVFWLFSGISLLGTVFVFLVVPETKGKSLNDIQRLLAGEKLNADEPAGLPEPRDERDERPAQVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V361</accession>
    <name>A0A6E8V361_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="2">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="2"/>
    <dbReference type="RefSeq" id="XP_040222269.1">
        <property type="nucleotide sequence ID" value="XM_040366335.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8V361"/>
    <dbReference type="EnsemblMetazoa" id="ACON001052-RA">
        <property type="protein sequence ID" value="ACON001052-PA"/>
        <property type="gene ID" value="ACON001052"/>
    </dbReference>
    <dbReference type="GeneID" id="120949189"/>
    <dbReference type="CTD" id="9099"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001658"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001052"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033140"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02674">
        <property type="entry name" value="Peptidase_C19R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000083">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF23">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE USP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026098662" description="ubiquitinyl hydrolase 1" evidence="3">
        <location>
            <begin position="21"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="4">
        <location>
            <begin position="12"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001052-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="339" mass="39094" checksum="BF5EE8AB05E7C9D6" modified="2020-08-12" version="1">MLRLCGIITGLCGLWNIGNTCFMNSMIQCLSHTRELTSFLRGQSSSERGTSKDHLILAEYTKLIKNMWSGTQRSINPSDLKYAFSSKHRMYSGSAQQDAQEFLRFFLDSLHGALNAAIKRDPLSEIDDNMNNKDKADMLWEWYSKAENSMIKDLFVGQLRSTLKCTFCNMESTMFDPFWDLSLPLPSSSSRCKLENCLEMFIKEEIMDGIDQPTCSNCDTRRKCTKRLTIERFPRYLVIHLKRFSEARWSKLTNIVEFPTKNRALNLQPYASEDISGPIYYSLYGISNHMGSTAGGHYVAVCKHPQTQQWNEFNDNYVTEAFERNLVTSNAYVLFYERA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V364</accession>
    <name>A0A6E8V364_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Troponin I</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the troponin I family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_049463041.1">
        <property type="nucleotide sequence ID" value="XM_049607084.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8V364"/>
    <dbReference type="EnsemblMetazoa" id="ACON001053-RC">
        <property type="protein sequence ID" value="ACON001053-PC"/>
        <property type="gene ID" value="ACON001053"/>
    </dbReference>
    <dbReference type="GeneID" id="120948222"/>
    <dbReference type="CTD" id="32794"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007438"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001053"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039909"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005861">
        <property type="term" value="C:troponin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006936">
        <property type="term" value="P:muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.350:FF:000004">
        <property type="entry name" value="WupA, isoform I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001978">
        <property type="entry name" value="Troponin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050875">
        <property type="entry name" value="Troponin_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038077">
        <property type="entry name" value="Troponin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13738">
        <property type="entry name" value="TROPONIN I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13738:SF1">
        <property type="entry name" value="TROPONIN I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00992">
        <property type="entry name" value="Troponin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90250">
        <property type="entry name" value="Troponin coil-coiled subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="2" id="KW-0488">Methylation</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00074692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001053-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="197" mass="23554" checksum="FCEF6D26BBA1BA7D" modified="2020-08-12" version="1">MADDEAKKAKQAEIERKRAEVRKRMEEASKAKKAKKGFMTPERKKKLRLLLRKKAAEELKKEQERKAAERRRIIEERCGKPKNVEDANEDQARKILRDYHQRINSLEEEKYDLEYVVKRKDMEISDLNAQVNDLRGKFVKPTLKKVSKYENKFAKLQKKAAEFNFRNQLKVVKKKEFTLEEEDKEAKKSEKADWQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V368</accession>
    <name>A0A6E8V368_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Protein kinase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8V368"/>
    <dbReference type="EnsemblMetazoa" id="ACON003005-RA">
        <property type="protein sequence ID" value="ACON003005-PA"/>
        <property type="gene ID" value="ACON003005"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001752"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003005"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_043000"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050321">
        <property type="term" value="F:tau-protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14338">
        <property type="entry name" value="UBA_SIK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000154">
        <property type="entry name" value="Serine/threonine-protein kinase SIK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057380">
        <property type="entry name" value="UBA_SIK1/2/3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF74">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23312">
        <property type="entry name" value="UBA_SIK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="1"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="309"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="528"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="631"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1076"/>
            <end position="1102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="349"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="564"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="642"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="1076"/>
            <end position="1085"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1086"/>
            <end position="1102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003005-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1188" mass="129777" checksum="CD5F88E69BC275A6" modified="2020-08-12" version="1">VAIKIIDKSQLDPGNLQKVYREVEIMKRLDHPHVIKLYQVMETQSMIYIVSEYASQGEIFDYIAKYGRLNERAARNKFWQILSAVEYCHNKGIVHRDLKAENLLLDSKMDIKIADFGFSNFYKKGELLATWCGSPPYAAPEVFEGKRYTGPEIDIWSLGVVLYVLVCGALPFDGSSLQSLRDRVLSGRFRIPFFMSSDCESLIRKMLVLDPSRRFSIDQIKRHRWMMVEIIDTPKISSIVINGSVSEVSALETEPNEQILKIMQNLGIDILKTRESLKLHSYDHYTAFYLLLLERLKSRTVSHENATTVAGCIGGGKSGMHESQRRRPSNVAEQAMRKLAISAQHKSDQPSSSPKHQQVSSGLNNHSSETLHGLLSAAQQVGSGVSPALPAMAPGMMLLRDTSIREQQPVLKDSSYFRGVSYTSSSGFTDASLYQLASLRERDCSSTYLTGTVGLLPSSLSSNVAGTLAAAAAAGASSSSARESNSIVLRESGILSSRISSSRLLSSNIDKRILQQSTEDCRRLLQQARPVSMGESNRYTKPPSHSPVNSDLHQTAAPQPPSQSSPAQSSAVPQSQRYSDPLNGFSKDYLTSASSNAETSITVPPFKDYLNNMQTYSYLQHYEPNLTVASSVASGHTPPPATSITAQYSSSTDEGCETDLGDEDVQQSIDKSIQRLNSFASSSSSSGVVTNIHPKSLSQNLSCDSSRSNFSTFESLDLNLSDCAELAGSLPSCTATTEAYESVAKDEATFRAVTSAACINQPQCVYAMSDKVVSSFLRANTVYQDVHNGDHRSITRSPVDFREGRRASDGLVTQGLVHASDHPLNSPVAFNSQRLNETCKAKGVLELHLLQKEAAQLKVKYQANVPQDEINVRQLQHSQFRVNPLDGLLLKPLAGSAHGSACLDPASGGTATGYYNKVADYVALSLGVKGGCAGSAGGQPEPALGKLEAEQLLLRAGVARSDQLSAAAVQQLQQKPPLQQQLMQHRLLQQKRQILQKQGAMEAGLSRRQMLRQHSYKIAQQTQILPPLPYGEMGEPGDGPAGGYPTLQSVRETAILETEPGQTLKEQLELYTHLQAHHSHMHHQQQQHQQQQQHGLGAGSHLGADRGALCSWNTLPSSMKTCQISDGAPATDSPWNVAALYHQNVPPAGPLYSPSHWITPHSSSIQHSMQLPLSESPIPELAEQMESI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V371</accession>
    <name>A0A6E8V371_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">26S proteasome regulatory subunit 6B</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8V371"/>
    <dbReference type="EnsemblMetazoa" id="ACON003008-RB">
        <property type="protein sequence ID" value="ACON003008-PB"/>
        <property type="gene ID" value="ACON003008"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000899"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003008"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039377"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000502">
        <property type="term" value="C:proteasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19502">
        <property type="entry name" value="RecA-like_PAN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000018">
        <property type="entry name" value="26S protease regulatory subunit 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000046">
        <property type="entry name" value="26S protease regulatory subunit 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000033">
        <property type="entry name" value="26S protease regulatory subunit 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050221">
        <property type="entry name" value="26S_Proteasome_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001270">
        <property type="entry name" value="ClpA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032501">
        <property type="entry name" value="Prot_ATP_ID_OB_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23073">
        <property type="entry name" value="26S PROTEASOME REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16450">
        <property type="entry name" value="Prot_ATP_ID_OB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00300">
        <property type="entry name" value="CLPPROTEASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0647">Proteasome</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="11">
        <location>
            <begin position="153"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003008-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="373" mass="42247" checksum="ABF2EAB272CE3A21" modified="2020-08-12" version="1">KLQKMLEFLEVQEEYIKDEQRNLKKEYLHAQEEVKRIQSVPLVIGQFLEAVDQNTGIVGSTTGSNYYVRILSTIDRELLKPSASVALHKHSNALVDVLPPEADSSISMLNADEKPDVQYSDIGGMDMQKQEIREAVELPLTHFELYKQIGIDPPRGVLMYGPPGCGKTMLAKAVAHHTTAAFIRVVGSEFVQKYLGEGPRMVRDVFRLAKENSPAIIFIDEIDAIATKRFDAQTGADREVQRILLELLNQMDGFDQTTNVKVIMATNRADTLDPALLRPGRLDRKIEFPLPDRRQKRLIFSTITAKMNLSEDVDLEDFVARPDKISGADINAICQEAGMHAVRENRYIVLAKDFEKGYKNNIKKDETEHEFYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V377</accession>
    <name>A0A6E8V377_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="10">BTB domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8V377"/>
    <dbReference type="EnsemblMetazoa" id="ACON001073-RB">
        <property type="protein sequence ID" value="ACON001073-PB"/>
        <property type="gene ID" value="ACON001073"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002202"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_014181"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001073"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035382"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048813">
        <property type="term" value="P:dendrite morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030707">
        <property type="term" value="P:follicle cell of egg chamber development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061061">
        <property type="term" value="P:muscle structure development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007423">
        <property type="term" value="P:sensory organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18315">
        <property type="entry name" value="BTB_POZ_BAB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000118">
        <property type="entry name" value="Abrupt, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051095">
        <property type="entry name" value="Dros_DevTransReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110">
        <property type="entry name" value="BTB DOMAIN TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110:SF106">
        <property type="entry name" value="FI01104P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="8">
        <location>
            <begin position="31"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="499"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="291"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="523"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="523"/>
            <end position="536"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001073-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="542" mass="61232" checksum="E7E868471274D874" modified="2020-08-12" version="1">MNPQQFSLRWNNYTSYIAGAFDSLRYEEDFVDVTLCCEGRKIRAHKILLSACSPYFKDVFKENPCQHPVIIFKNVRYTDLMSLVEFMYQGEVSVPQEQLPSFLHTAEILAIRGLTDNTSDSRQPASTTTSAIAQQLIQSQAVLEKPATITTTDSLYLTLPSNSTIVHQPKLVQAQIQTTPIITKPLIKNTPTEMPSLAASSTLAVPIAQQQQPAIAKIQVQASSQSQQPQQVQVQVQQVQQTQTHQTQQATTSQTQSQSQQATTLQEVVENVVLPRKKKVKGQQIIASQSNTSIPSGSTVTVTTTTTGQSGDNEMYETDTYTITTKDAREDHEQAEQYVESGQSSGTTLKMEIPEFISVGEPINTGNGTNNTYLQVTGSLSQHVTIIVFENEFKTHFKQYQYLYSSSYDIFLSSLLQKSTYEAEARENYIIIGSLRSYPATWLLPALMLLTRGHHRVLSIVTDIVSENKWSRCKICRLVITASNLWRHVRTQHSRLEPKRCEHCKKQFKNKYSLREHVRITHESKQPREENIKQHNIDQTTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V378</accession>
    <name>A0A6E8V378_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Dynamin</fullName>
            <ecNumber evidence="2">3.6.5.5</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.5"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.5" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8V378"/>
    <dbReference type="EnsemblMetazoa" id="ACON003018-RB">
        <property type="protein sequence ID" value="ACON003018-PB"/>
        <property type="gene ID" value="ACON003018"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010238"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003018"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042070"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:programmed cell death"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031623">
        <property type="term" value="P:receptor internalization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016185">
        <property type="term" value="P:synaptic vesicle budding from presynaptic endocytic zone membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08771">
        <property type="entry name" value="DLP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01256">
        <property type="entry name" value="PH_dynamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000370">
        <property type="entry name" value="Dynamin, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000045">
        <property type="entry name" value="dynamin-1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1240:FF:000008">
        <property type="entry name" value="dynamin-3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1240">
        <property type="entry name" value="Dynamin, middle domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022812">
        <property type="entry name" value="Dynamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001401">
        <property type="entry name" value="Dynamin_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019762">
        <property type="entry name" value="Dynamin_GTPase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045063">
        <property type="entry name" value="Dynamin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000375">
        <property type="entry name" value="Dynamin_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030381">
        <property type="entry name" value="G_DYNAMIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003130">
        <property type="entry name" value="GED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020850">
        <property type="entry name" value="GED_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566">
        <property type="entry name" value="DYNAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566:SF212">
        <property type="entry name" value="DYNAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01031">
        <property type="entry name" value="Dynamin_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00350">
        <property type="entry name" value="Dynamin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02212">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00195">
        <property type="entry name" value="DYNAMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00053">
        <property type="entry name" value="DYNc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00302">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00410">
        <property type="entry name" value="G_DYNAMIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51718">
        <property type="entry name" value="G_DYNAMIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51388">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="8 13" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="9" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynamin-type G" evidence="17">
        <location>
            <begin position="23"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="15">
        <location>
            <begin position="513"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="domain" description="GED" evidence="16">
        <location>
            <begin position="656"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="621"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="749"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="804"/>
            <end position="815"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048040"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00067339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003018-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="862" mass="96199" checksum="3034B4A51B16E1FA" modified="2020-08-12" version="1">MESLIPIVNKLQDAFTQMGVHMQLDLPQIAVVGGQSAGKSSVLENFVGRDFLPRGSGIVTRRPLILQLINGTVEFGEFLHQKGKKFSNFEEIRQEIEAETDRITGSNKGISNIPINLRVYSPHVLNLTLIDLPGLTKVPIGDQPADIENQIKGMIFQFIRKETCLILAVTPANTDLANSDALKLAKEVDPQGVRTIGVITKLDLMDEGTDARDILENKLLPLRRGYIGVVNRSQKDIEGRKDIHAALAAERKFFLSHPSYRHIADRLGTPYLQKVLNQQLTNHIRDTLPALRDRLQKQMLTLEKDVDQYKHFRPDDPSIKTKAMLQMIQQLQSDFERTIEGSGSALVNTNELSGGAKINRIFHERLRFEIVKMSCDEKELRREISFAIRNIHGIRVGLFTPDMAFEAIVKKQISQLKEPILKCVDLTVLELSNVVRICTDKMARYPRLRDETERIITTHIRQCEQKAKEQMMLLIDYELAYMNTNHEDFIGFANAQSKTENAVKTGTRNLGSQVIRKGHMCIQNLGIMKGGSRPYWFVLTSESISWFKDEDEKEKKFMLPLDGLKLRDIEQGFMSRRHTFGLFNPDGRNVYKDYKQLELSCESTDDVDSWKASFLRAGVYPEKDTPANGDETEAEESGGESGPTGQSLDPQLERQVETIRNLVESYMRIVTKTTRDMVPKAIMMLIINNTKDFINGELLAHLYATGDQASMMEESADEAQKREEMLRMYHACKEALRIIGDVSMATFSTPVPPPVKNDWLSSGLDNPRLSPPSPGGPRKAAPQQQGSLSSMGAPAGRGPPPAPASARPAPAIPNRPGGGAAPPLPGGRPGGQALPAPLIPSRPAQNAPPRLPDRPYNAMGRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V4W0</accession>
    <name>A0A6E8V4W0_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8V4W0"/>
    <dbReference type="EnsemblMetazoa" id="ACON002250-RB">
        <property type="protein sequence ID" value="ACON002250-PB"/>
        <property type="gene ID" value="ACON002250"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_014193"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002250"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040760"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061762">
        <property type="term" value="P:CAMKK-AMPK signaling cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:002487">
        <property type="entry name" value="RE68077p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895">
        <property type="entry name" value="CALCIUM/CALMODULIN-DEPENDENT PROTEIN KINASE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895:SF164">
        <property type="entry name" value="CALCIUM_CALMODULIN-DEPENDENT PROTEIN KINASE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="1"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="325"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="389"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="342"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002250-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="413" mass="45111" checksum="24B41B2226512165" modified="2020-08-12" version="1">MKILSKRKLLRKAGLMRRGPKRGTSPLDRVYREIAVLKKLDHPNVVKLVEVLDDPLEDSLYLVFELVQQGEVLSIPTETPLSEERAWNVFRDVLLGVEYLHYQRIIHGDLKPANLLLSDSGSVKVADLGVCNEFLGEDAAMNNGSTAGTPAFRAPETLLPGQHFYNGKAADIWALGATLFSLVHGNVPFIATSVPGVYEKIKNDPLEFPANCSSAAASTISEELRDLISRMLDKDPQQRITLPQIKEHPWMTKHGTVRLPSEEENCRLVQISDEDMSSVVKSIPKLDTLILIKTMLKKHSFQNPFTKGISGRAPQAGGSRLERFCRSGRSNSAPGDYHTSERQPSSESLLPSVTEGVTSPLGSPDATSTVATITTGSIISELDSLTLRSDVSCPSSSTDNDTATVTIITTPPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V4W5</accession>
    <name>A0A6E8V4W5_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">choline-phosphate cytidylyltransferase</fullName>
            <ecNumber evidence="10">2.7.7.15</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">Ngousso</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="9">Phospholipid metabolism; phosphatidylcholine biosynthesis; phosphatidylcholine from phosphocholine: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytidylyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.15" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8V4W5"/>
    <dbReference type="EnsemblMetazoa" id="ACON002263-RD">
        <property type="protein sequence ID" value="ACON002263-PD"/>
        <property type="gene ID" value="ACON002263"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010575"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002263"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035988"/>
    <dbReference type="UniPathway" id="UPA00753">
        <property type="reaction ID" value="UER00739"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004105">
        <property type="term" value="F:choline-phosphate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031210">
        <property type="term" value="F:phosphatidylcholine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02174">
        <property type="entry name" value="CCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000016">
        <property type="entry name" value="Putative choline-phosphate cytidylyltransferase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041723">
        <property type="entry name" value="CCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045049">
        <property type="entry name" value="Pcy1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10739:SF13">
        <property type="entry name" value="CHOLINE-PHOSPHATE CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10739">
        <property type="entry name" value="CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="7" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cytidyltransferase-like" evidence="12">
        <location>
            <begin position="67"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="283"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="302"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="312"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="354"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="384"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="394"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00026101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002263-PD"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="416" mass="46884" checksum="75DC8492A954AD0B" modified="2020-08-12" version="1">MAPNYLPTESDGPVQITVQHRLDTICKQAPYSTELDAIRERERCDYTQKITYQMARAGTAPRMIRVYADGIYDLFHQGHARQLMQAKNVFPNVYLIVGVCNDKLTHSRKGRTVMNDEERYEAVRHCRYVDEIVPDAPWELDDEFIEKHKIDFVAHDEIPYSTDDCNDVYAKIKAKGMFVATERTEGVSTSDIVARIVKDYDIYVRRNLARGYTAKELNVSFLSEKKFRFQNKMVELKDKVRKGKDDFITKWEERSTDLIKTFLLMFGKERISMFLSDSKQRIRSALSPPGSPGSGSNHSLNDCDDDDDLESPDSVLDSPPSKRSNVIRSRHHLLSEEDDEEQGLGGPRSPPPVLEDDEDDDDDDEAYLDSRSRPSSNYNISLIAGGGDGGSSGGSAALNSSSSGGSGSRTYGNRNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V4W8</accession>
    <name>A0A6E8V4W8_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Ankyrin 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON002272-RA">
        <property type="protein sequence ID" value="ACON002272-PA"/>
        <property type="gene ID" value="ACON002272"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000169"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004581"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002272"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030750"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033660"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08317">
        <property type="entry name" value="Death_ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.220.30:FF:000009">
        <property type="entry name" value="Ankyrin 2, isoform G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000095">
        <property type="entry name" value="Ankyrin 2, isoform J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000003">
        <property type="entry name" value="Ankyrin, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000001">
        <property type="entry name" value="Ankyrin-2 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.220.30:FF:000001">
        <property type="entry name" value="Ankyrin-3 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.2660:FF:000001">
        <property type="entry name" value="Ankyrin-3 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.220.30">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2660">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040745">
        <property type="entry name" value="Ankyrin_UPA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000488">
        <property type="entry name" value="Death_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051165">
        <property type="entry name" value="Multifunctional_ANK_Repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000906">
        <property type="entry name" value="ZU5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24123:SF141">
        <property type="entry name" value="ANKYRIN 2, ISOFORM U"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24123">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13637">
        <property type="entry name" value="Ank_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00531">
        <property type="entry name" value="Death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17809">
        <property type="entry name" value="UPA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00791">
        <property type="entry name" value="ZU5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="23"/>
    </dbReference>
    <dbReference type="SMART" id="SM00005">
        <property type="entry name" value="DEATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00218">
        <property type="entry name" value="ZU5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="21"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="21"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50017">
        <property type="entry name" value="DEATH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51145">
        <property type="entry name" value="ZU5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="9">
        <location>
            <begin position="37"/>
            <end position="69"/>
        </location>
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</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
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        <name type="scientific">Anopheles coluzzii</name>
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        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
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    <reference evidence="5" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
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        <scope>IDENTIFICATION</scope>
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            <begin position="58"/>
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    </evidence>
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    </evidence>
    <sequence length="187" mass="20946" checksum="2E6CFE8AFAB485BF" modified="2020-08-12" version="1">MADNANKRNIPIKLGDFSVIDTEFSSIRERFDSEMKKMEEEMARFRSDLMHREPNFFETTSSSTTSNAATSMKLPQSQSGSDICSPLIQDDGDGKVLKLRFDVSQYAPEEIVVKTVDNKLLVHAEHAEKSDTKSVYREYNREFMLPKGCLPENIKSSLSKDGVLTVDAPIQPEALLAGETMVPIAHN</sequence>
</entry>
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    <accession>A0A6E8V9B6</accession>
    <name>A0A6E8V9B6_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CRK SH3-binding GNRP</fullName>
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    </protein>
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        <name type="scientific">Anopheles coluzzii</name>
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            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="EnsemblMetazoa" id="ACON000932-RA">
        <property type="protein sequence ID" value="ACON000932-PA"/>
        <property type="gene ID" value="ACON000932"/>
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    <dbReference type="VEuPathDB" id="VectorBase:ACON000932"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037502"/>
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        <property type="component" value="Unplaced"/>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:Ras protein signal transduction"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="REM"/>
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        <property type="entry name" value="rap guanine nucleotide exchange factor 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.870.10">
        <property type="entry name" value="Son of sevenless (SoS) protein Chain: S domain 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Ras-like_GEF"/>
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        <property type="entry name" value="Ras-like_Gua-exchang_fac_N"/>
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        <property type="entry name" value="Ras_GEF_dom_sf"/>
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        <property type="entry name" value="RASGEF_cat_dom"/>
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        <property type="entry name" value="RASGEF_cat_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RAP GUANINE NUCLEOTIDE EXCHANGE FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00618">
        <property type="entry name" value="RasGEF_N"/>
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        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00229">
        <property type="entry name" value="RasGEFN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48366">
        <property type="entry name" value="Ras GEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50009">
        <property type="entry name" value="RASGEF_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50212">
        <property type="entry name" value="RASGEF_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-terminal Ras-GEF" evidence="6">
        <location>
            <begin position="1112"/>
            <end position="1233"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GEF" evidence="5">
        <location>
            <begin position="1262"/>
            <end position="1487"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
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            <begin position="393"/>
            <end position="412"/>
        </location>
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            <begin position="1"/>
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            <begin position="164"/>
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    </feature>
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            <begin position="1057"/>
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            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00083313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
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            <dbReference type="PROSITE" id="PS50212"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000932-PA"/>
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    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1495" mass="161724" checksum="899F7AA9447E9182" modified="2023-06-28" version="2">MTRSHSPNSPRGGAGGSGANAASYGSRKSTTDEPAKPVQDLNYHVRQVKNALTHFKDVILKNKLEMLPGNGTVVLESIANVHTALQSYTLNEHSSAFINATNHVHVSLGNLLKLCDEVLLTKEGDDCPSLSKENVKEVVELVENAVNNLVNLANEKLSDRKAIGTGGGGGPGTGVSGTAAGHSTGGPSSNTLQRPTVDVVSQRTSLPDIPLTPRERDILEQTSLKTVRASHSTESILRDSSPPPPPKPPLPDRSQEPPPPLPPKRKSQHAKNHSFHDTTTASSDCASTDSTIFQLGGGPGSSAGGATGAGGSIGLDRMSLRSRSPEDNCSLLSASAGSLDSALNHSREEDELRALTSCSSHASATAASLGGLVILGAGAAGCGTQHWEEAGDLAGGLPQHSNNSLNRNSNESGFESMYSLRVSRDQHQQQQQQQQMHMQYQSTAATVTTHHHHQKSASSSSSSSSYVHKSESIVDGMAALVVASGKGAVQQQHLQQHHQAHVLHHQQHQQQQQQHFHQKIDTIITQASDDSSAGPKGASEQLASATSLTSTVTTSSSSSLSKLLVHGPSIDELGNDDVFPRTSAGGATDRPPALPVKTRSHSIKRERHPSQYDNVDEVDLDRGSQDSFGHFPMQSSPSYLYSRQQMFHNLPSKSYHISLIEPRHMSRFQEEPPPLPIKKKHMFQSVAYSVMAYMEIFGSATQNQSEFMRHSVHTYNLAHSEQISTSSTTSNHSLSHSQTMSLSPSRIAPATVSPPSSPNVSVKPPALPPKRQRINSKTPSIASTPPPSPKIFQDQQQHHHAQHHYHQQQAQAPNVINTSPSPSTSSLASTQSGAGSKPSAVCNQTLDVKTPKVAQPPLAASAPEAEPATASGSAASTSTTSTTLTNIAATTTTTTTISSVGDDGGAAAAGAATEQCGIRYHSPNRRHPAGGQHAADDDYLHLCDATTRAPIAGDNLSASSSSAFPSSVSNLNLASTAGRGSGSTSQHLVSVTNHSHSNNTSGSSSNNVESSSYLNDSHNKGRTSSSSSGGQAVLPSSDAMIYEASTTQSPLPDGDAGDERASNKDGDEVEVILRRNNKNGMTMAMELQQQPLNLMEELDVSNYLVFKKENEDGPDVKGGHPDALIIHATRVQKNSDAYGEAFITTFRTFISPLDLIQKLSHRYTVYHCQMNDAKQKAAKESFSLLVRVVNDLTTPDLSERLLVILMNFDYQLVSAGHLTMAKLLRVKLIEKALIYKQKASLTVPTLSSRALVAQPPTLLDLKSAEIAEQMTLLDAELFQKIEIPEVLIWAQEQCEERSPNLTRFTEHFNKMSYWARTQILSQNDAKDREKHVIKFIKIMKHLRKINNYNSYLALLSALDSAPIRRLEWHKTITEGLKEYCALIDSSSSFRAYRQALAETNPPCIPYIGLVLQDLTFVHIGNPDLLPDGSTNFSKRWQQYHIVVNMKRFKKGSYPFKKNERIIGFFDNFEYYLDEDAMWQISETIKPRGSRKANVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VAP5</accession>
    <name>A0A6E8VAP5_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="13">C2H2-type domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040226194.2">
        <property type="nucleotide sequence ID" value="XM_040370260.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VAP5"/>
    <dbReference type="EnsemblMetazoa" id="ACON002083-RA">
        <property type="protein sequence ID" value="ACON002083-PA"/>
        <property type="gene ID" value="ACON002083"/>
    </dbReference>
    <dbReference type="GeneID" id="120951496"/>
    <dbReference type="KEGG" id="acoz:120951496"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_013645"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002083"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030802"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000158">
        <property type="entry name" value="Zinc finger protein 362"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000648">
        <property type="entry name" value="Zinc finger protein rotund"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001172">
        <property type="entry name" value="Zinc finger protein rotund"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001678">
        <property type="entry name" value="Zinc finger protein rotund"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394:SF48">
        <property type="entry name" value="ZINC FINGER PROTEIN 771"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="528"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="557"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="585"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="613"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="643"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="674"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="65"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="118"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="173"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="213"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="473"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="690"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="774"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="77"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="118"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="135"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="220"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="237"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="262"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="281"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="475"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="485"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="695"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="708"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="780"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="807"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="854"/>
            <end position="869"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002083-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="999" mass="107039" checksum="E9DC6784C8F3B613" modified="2020-08-12" version="1">MTSSKLRIKDDLYNRLSPVQTDEMLYKETTKTRSSYGYDHQDSCERPLRYAEECPTQTKFSTKHLTNTSSHLHHDADDDDGDEDDDTTDTEMPPKSAFILRAQFYQNLFAASANLLKKQRPTKRHRVPAPASDGFEADASGDDAGIDEKAEDLSSPLETALAGVGQLQKCLFPSAPAPHGRQESNGGNGAASEVGCDVDTADEMLEKPSNAFPWLSEATNNNDYTKGNNNHHGTPPHLHHPHHPHHHHHHHHHPHHSHHLTHSAAAAAAAAAAAATSSGTQSSVGPASSSTGNCRHPEDTKCRALNCGGGGPPQSPSAGSSLAANEGATPDSSSTTATATAIPNFNQMPVPGGVQGQNPTQGLVHWMSAVMAEHMTSNPHHDPAAVGMHYMWNGPVEQCGQHTKDMDTYGGWPAPRNPMSMKQGYEAKMNPVDHHHHHHHHHHNNLQKGHMIDDGRLLDHHTMQTGAAQMSQLYGARGSSSSSSPGGGLGHAGGGPTNPAAALLVVPQPINATKIGATGLPNGTGRKYQCKMCPQIFTSKADLQLHTQIHMREAKPYKCSQCNKAFANSSYLSQHTRIHLGIKPYRCEICQRKFTQLSHLQQHIRTHTGDKPYKCRHSGCLKAFSQLSNLQSHSRCHQTDKPYKCNSCYKCFADEPALLEHIPKHKESKHLKTHICQYCGKSYTQETYLSKHMQKHAERTDKRPPIGTRSSSSTVNLSASTPGSSSSSGSASNGATSNGGAATSMAGESAFWPKVSPDSAAATINDVINQQNNHHEFSLTPNGSATNGSTGNGPQSNGTDHPGGGPPQQQQQQQQQHHNQRDDAVDEMVGPAAARGPPDSVVGNGAPNGGNAGSGAANQASGQQQQQQQQPPPQQQHNGTMNGGNLAIPPVSTPMSYDTGSIAKATSNSAFTPINAMPPHLNGLQHHHQLATQRPSYLYDAISFQNQKAAMSQSPSNAFPNQLISLHQIRNYAHQPAGGLMAGEHLLGVTVGPGGKDKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VAQ2</accession>
    <name>A0A6E8VAQ2_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Extremely potent competitive inhibitor of cAMP-dependent protein kinase activity, this protein interacts with the catalytic subunit of the enzyme after the cAMP-induced dissociation of its regulatory chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PKI family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VAQ2"/>
    <dbReference type="EnsemblMetazoa" id="ACON001779-RB">
        <property type="protein sequence ID" value="ACON001779-PB"/>
        <property type="gene ID" value="ACON001779"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010923"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001779"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038127"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004862">
        <property type="term" value="F:cAMP-dependent protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004171">
        <property type="entry name" value="cAMP_dep_PKI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15416">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE INHIBITOR/PKI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02827">
        <property type="entry name" value="PKI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0649">Protein kinase inhibitor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="14"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="83"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001779-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="118" mass="11913" checksum="8438ED02269AB690" modified="2020-08-12" version="1">LSIIKLATMDKLRESGAADDPTTQQRLATDGQGGTAAAAGGEGTSDEYHHQFYNTGRIGRRNALPDILGTHCTTTTADLSSQLGSLSTSDCAGKASDGSSSCIAAGNNGQPPPTTTAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VAR0</accession>
    <name>A0A6E8VAR0_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ninjurin-2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ninjurin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040221004.1">
        <property type="nucleotide sequence ID" value="XM_040365070.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VAR0"/>
    <dbReference type="EnsemblMetazoa" id="ACON002124-RB">
        <property type="protein sequence ID" value="ACON002124-PB"/>
        <property type="gene ID" value="ACON002124"/>
    </dbReference>
    <dbReference type="GeneID" id="120948573"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012474"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002124"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_036200"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042246">
        <property type="term" value="P:tissue regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007007">
        <property type="entry name" value="Ninjurin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12316:SF17">
        <property type="entry name" value="NINJURIN C, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12316">
        <property type="entry name" value="NINJURIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04923">
        <property type="entry name" value="Ninjurin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="64"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="106"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002124-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="139" mass="15003" checksum="2114F33AE453440A" modified="2020-08-12" version="1">MAMASILPASSGVGAESDRVLKSMDANRYATKKTIAQGMLDIALLTANASQLKYILQVGEKHEFYGLMLTLISISIILQVSAAMLAVILTLVNLHGGEVQRKVANILNHFSLGFVVVALFCDVIKMNFGLDPAIPYIHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VAR8</accession>
    <name>A0A6E8VAR8_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Junctophilin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_040220103.1">
        <property type="nucleotide sequence ID" value="XM_040364169.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VAR8"/>
    <dbReference type="EnsemblMetazoa" id="ACON002159-RA">
        <property type="protein sequence ID" value="ACON002159-PA"/>
        <property type="gene ID" value="ACON002159"/>
    </dbReference>
    <dbReference type="GeneID" id="125906388"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001327"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002159"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038530"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002159-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="129" mass="15036" checksum="256CAC36D3164BD3" modified="2020-08-12" version="1">MQWQQEVDDEDLMYSPALLARRASESWIEFPPVESIPVNATLQRKKSLPDVQQLPFATGAMSREEVSILGSARREEVRRLRDEQERLRVNPLLYLVSPQVRDWFSRQQLVMLVLIVNIALAILFFKILT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VAS7</accession>
    <name>A0A6E8VAS7_ANOCL</name>
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        <submittedName>
            <fullName evidence="5">Tropomyosin</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the tropomyosin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VAS7"/>
    <dbReference type="EnsemblMetazoa" id="ACON001797-RI">
        <property type="protein sequence ID" value="ACON001797-PI"/>
        <property type="gene ID" value="ACON001797"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002131"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001797"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039487"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000005">
        <property type="entry name" value="Tropomyosin alpha-1 chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000001">
        <property type="entry name" value="Tropomyosin alpha-1 chain isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000533">
        <property type="entry name" value="Tropomyosin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19269">
        <property type="entry name" value="TROPOMYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00261">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00194">
        <property type="entry name" value="TROPOMYOSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00326">
        <property type="entry name" value="TROPOMYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="279"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="77"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="288"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="311"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="358"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="375"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="443"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001797-PI"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="453" mass="48804" checksum="B29E790BDEAFDF6A" modified="2020-08-12" version="1">MAANVQQQSGNLLDVLKKKMRQTKEEMEKYKDECEEFHKKLQNEVMRREEAESEVAALNRRIQLLEEDLERSEERLASATAKLSEASAAADESERARKVLENRALADEERMDALENQLKEARFMAEEADKKYDEVARKLAMVEADLERAEERAEAGEAKIVELEEELRVVGNNLKSLEVSEEKANQREEEYKNQIKTLTTRLKEATQREESYGGQVRVLDQRLKEAEARAEFAERSVQKLQKEVDRLEDELIVEKLRYAEIGDDLDFAFVDLIPGIDPIWTERRPKPKTPPPPPKLPTPPPPPPAPKEEAAAAAGGEGEAAAAGGEGAPAGPTKARTPSPFELRKHFPPEAAEVPYVRSSTGTTPKSSPAKEVAPAEAAPAPEAAAPAEAPAEGGADAAAPPAEAAAPAAEGEAPAAPAAEGEAAAPAPEAAAAPAEEAAPPAAEPAPEAPAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VAS9</accession>
    <name>A0A6E8VAS9_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_040230064.2">
        <property type="nucleotide sequence ID" value="XM_040374130.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VAS9"/>
    <dbReference type="EnsemblMetazoa" id="ACON001800-RA">
        <property type="protein sequence ID" value="ACON001800-PA"/>
        <property type="gene ID" value="ACON001800"/>
    </dbReference>
    <dbReference type="GeneID" id="120953833"/>
    <dbReference type="CTD" id="6860"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_013758"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001800"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042945"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070382">
        <property type="term" value="C:exocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001786">
        <property type="term" value="F:phosphatidylserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048791">
        <property type="term" value="P:calcium ion-regulated exocytosis of neurotransmitter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08388">
        <property type="entry name" value="C2A_Synaptotagmin-4-11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08404">
        <property type="entry name" value="C2B_Synaptotagmin-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000039">
        <property type="entry name" value="Synaptotagmin 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000181">
        <property type="entry name" value="Synaptotagmin 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001565">
        <property type="entry name" value="Synaptotagmin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10024:SF369">
        <property type="entry name" value="FI18813P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10024">
        <property type="entry name" value="SYNAPTOTAGMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00360">
        <property type="entry name" value="C2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00399">
        <property type="entry name" value="SYNAPTOTAGMN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="14"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="4">
        <location>
            <begin position="168"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="4">
        <location>
            <begin position="329"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="45"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="99"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001800-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="473" mass="51390" checksum="FEE24CD62508807E" modified="2020-08-12" version="1">MGDHGPDMNTLETVVPAVLGLTAAAILAVTACFCARRFRRQHKKAGHEASSLPFQPPRPPKAVRSPSGQPPQYLKKSPSPTSIKPLPGHLPAQSPTDQTTAAGGTTAATTTTTLATTTTTTSIVPPTKYTEENELIPKNAQLEPKSPDVSELGDPSACENGDGTEHGKLGTIVFKLRFLADRSALVVSVVRCRGLPGKNHGTAAAELSTIGGGTMLCNGSANGKQTATDPYVKLQLLPDKQHKVKTRVLRNTRNPVYDEDFTFYGLTLSELAGMSLHFVVLSFDRYSRDDVIGEVVCPLSGIDLQQIENQQVALSREIQPRSLKIRAQGRGELLISLCWQPAAARLTVVLLKARNLPRMDVTGLADPYVKIYLLYNGQRIAKKKTHVKKRTLSPVFNESFAFDIPTTEGAGGATLDGVSLELMLLDWDRVTKNEVIGRLELGGPRSNGSALNHWKEVCNSPRRQIADWHKLRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VB18</accession>
    <name>A0A6E8VB18_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">tRNA (guanine(10)-N(2))-methyltransferase TRMT11</fullName>
            <ecNumber evidence="12">2.1.1.214</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">tRNA methyltransferase 11 homolog</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalytic subunit of the TRMT11-TRM112 methyltransferase complex, that specifically mediates the S-adenosyl-L-methionine-dependent N(2)-methylation of guanosine nucleotide at position 10 (m2G10) in tRNAs. This is one of the major tRNA (guanine-N(2))-methyltransferases.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>guanosine(10) in tRNA + S-adenosyl-L-methionine = N(2)-methylguanosine(10) in tRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43128"/>
            <dbReference type="Rhea" id="RHEA-COMP:10355"/>
            <dbReference type="Rhea" id="RHEA-COMP:10357"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74481"/>
            <dbReference type="EC" id="2.1.1.214"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:43129"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Part of the heterodimeric TRMT11-TRM112 methyltransferase complex; this complex forms an active tRNA methyltransferase, where TRMT112 acts as an activator of the catalytic subunit TRMT11.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM11 methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.214" evidence="12"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8VB18"/>
    <dbReference type="EnsemblMetazoa" id="ACON002384-RA">
        <property type="protein sequence ID" value="ACON002384-PA"/>
        <property type="gene ID" value="ACON002384"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_003133"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002384"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040313"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043527">
        <property type="term" value="C:tRNA methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160102">
        <property type="term" value="F:tRNA (guanine(10)-N2)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000468">
        <property type="entry name" value="SD03208p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000241">
        <property type="entry name" value="RlmKL-like_Mtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016691">
        <property type="entry name" value="TRMT11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059073">
        <property type="entry name" value="TRMT11_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13370">
        <property type="entry name" value="RNA METHYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13370:SF3">
        <property type="entry name" value="TRNA (GUANINE(10)-N2)-METHYLTRANSFERASE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25904">
        <property type="entry name" value="Tmrp11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01170">
        <property type="entry name" value="UPF0020"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017259">
        <property type="entry name" value="tRNA_mtfrase_TRM11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00507">
        <property type="entry name" value="N12N6MTFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51627">
        <property type="entry name" value="SAM_MT_TRM11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 15" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 15" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 15" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 15" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="3 15" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="tRNA (guanine(10)-N(2))-methyltransferase TRMT11 N-terminal" evidence="18">
        <location>
            <begin position="6"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosomal RNA large subunit methyltransferase K/L-like methyltransferase" evidence="17">
        <location>
            <begin position="192"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="420"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="420"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00050985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00056270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00065434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00066937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00067484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00075308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF01170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF25904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002384-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="459" mass="52728" checksum="BBF4B720387BE3D9" modified="2020-08-12" version="1">MSSTLKKYVLWFAQEHVDFRQAEIASLLRIWNIQMETPADHNPKRPFWVVGLQNDEAARKLASRSMSLRCIFELWAHSNEGLANFHQALRQHIETNAQSLATHFEANKSFKITVETYNKHFSQREKVAKIETMQYLPVEGPVNLKTPDVHYWYIEFWGLDPMDVPEQPYDVLFGRWVADGKRDMINEISLKTRKFIGNTSMDPQLSLLMANQGLVESGHLVFDPFAGSGSLLVAAAKFGAYVAGADIDYMIVHGKSKPTRVNQKVREKDESIYANLQQYGCGGLFVDVLIADFSRSIWADSIVFDSIITDPPYGIREATERIEFKTQRRATAMTEDAVHYPSTSPYQLCQMYKDLLQFSARHLKLGGRLVCWFPMLSSRRLLTYEKVSDSEADLGAFEMSATVVENFRERYFTQLAEDNGTRKERRLELREVGRKEAKKRGKQQQPDGKWRFPQDAPDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VB35</accession>
    <name>A0A6E8VB35_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">MARVEL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VB35"/>
    <dbReference type="EnsemblMetazoa" id="ACON002428-RB">
        <property type="protein sequence ID" value="ACON002428-PB"/>
        <property type="gene ID" value="ACON002428"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000371"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002428"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031098"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019991">
        <property type="term" value="P:septate junction assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008253">
        <property type="entry name" value="Marvel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038976">
        <property type="entry name" value="Ssk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36692:SF1">
        <property type="entry name" value="GH08457P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36692">
        <property type="entry name" value="PROTEIN SNAKESKIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01284">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="68"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="97"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="138"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="MARVEL" evidence="6">
        <location>
            <begin position="30"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002428-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="170" mass="18799" checksum="D2E1E6E0CCC82493" modified="2020-08-12" version="1">MANAESGADQADRGMRKEYFNSSGAGKFLPPVLKLLELAVAIVCIGLIDDPAHHSRLRVFISSRTVALAYGTFVLFLVFSVVYLFGKVIRDNFPWKLSSLLNLTGFILYLATAACILNDWSETKNRNYWPPNTQRMDFLCGAGSVAVVGALFYLIDLIVTVRLGMKGEIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VEX3</accession>
    <name>A0A6E8VEX3_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase</fullName>
            <ecNumber evidence="6">3.1.4.11</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6 9">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + H2O = 1D-myo-inositol 1,4,5-trisphosphate + a 1,2-diacyl-sn-glycerol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:33179"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
            <dbReference type="ChEBI" id="CHEBI:203600"/>
            <dbReference type="EC" id="3.1.4.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="8">Binds 1 Ca(2+) ion per subunit.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.11" evidence="6"/>
    <dbReference type="RefSeq" id="XP_040227488.1">
        <property type="nucleotide sequence ID" value="XM_040371554.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VEX3"/>
    <dbReference type="EnsemblMetazoa" id="ACON001936-RA">
        <property type="protein sequence ID" value="ACON001936-PA"/>
        <property type="gene ID" value="ACON001936"/>
    </dbReference>
    <dbReference type="GeneID" id="120952289"/>
    <dbReference type="CTD" id="31376"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007070"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001936"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032074"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004435">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate phospholipase C activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008344">
        <property type="term" value="P:adult locomotory behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043153">
        <property type="term" value="P:entrainment of circadian clock by photoperiod"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042592">
        <property type="term" value="P:homeostatic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046488">
        <property type="term" value="P:phosphatidylinositol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051209">
        <property type="term" value="P:release of sequestered calcium ion into cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00275">
        <property type="entry name" value="C2_PLC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13361">
        <property type="entry name" value="PH_PLC_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08591">
        <property type="entry name" value="PI-PLCc_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000024">
        <property type="entry name" value="1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1230.10:FF:000005">
        <property type="entry name" value="1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.240:FF:000001">
        <property type="entry name" value="1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000008">
        <property type="entry name" value="1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1230.10">
        <property type="entry name" value="Phospholipase C beta, distal C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001192">
        <property type="entry name" value="PI-PLC_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016280">
        <property type="entry name" value="PLC-beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042531">
        <property type="entry name" value="PLC-beta_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037862">
        <property type="entry name" value="PLC-beta_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053945">
        <property type="entry name" value="PLCB1-4-like_EFh"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000909">
        <property type="entry name" value="PLipase_C_PInositol-sp_X_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001711">
        <property type="entry name" value="PLipase_C_Pinositol-sp_Y"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336:SF36">
        <property type="entry name" value="1-PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE PHOSPHODIESTERASE BETA-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336">
        <property type="entry name" value="PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE C FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17787">
        <property type="entry name" value="PH_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00388">
        <property type="entry name" value="PI-PLC-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00387">
        <property type="entry name" value="PI-PLC-Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22631">
        <property type="entry name" value="PLCB1-4-like_EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000956">
        <property type="entry name" value="PLC-beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00390">
        <property type="entry name" value="PHPHLIPASEC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00148">
        <property type="entry name" value="PLCXc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00149">
        <property type="entry name" value="PLCYc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69989">
        <property type="entry name" value="C-terminal domain of PLC-beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50007">
        <property type="entry name" value="PIPLC_X_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50008">
        <property type="entry name" value="PIPLC_Y_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 6 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 6 9" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="4 6 9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="PI-PLC Y-box" evidence="12">
        <location>
            <begin position="544"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="11">
        <location>
            <begin position="660"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="1022"/>
            <end position="1078"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="328"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="375"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="329"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="358"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="360"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="409"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR000956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000956-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000956-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001936-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1089" mass="124753" checksum="27BB03DAFB072935" modified="2020-08-12" version="1">MTKKFEFNWQIPVPEPLLTGCVFDRWTEEKDNNELEPNCMFKVDEYGFFIYWKSEGREGDVIELCQVSDIRAGGMPKDMKLYNQLCNKHGENVEEKSLTICSGTDYININYQHVVCPDAATAKIWVDGLRKITHNGKANNFCPMTALKKHWMRLGFLVDPRGKVPVKVVARTFASGKTEKLVYQILSDLGLPSGKNDVIEKADFTFEKFYELYHKICPRNDIEELFRSITQGKANAINLDQFINFLNEKQRDPRLNEILYPLYDEKRALEIITTYEQNDEAREAKTLTKDGLIRYLMSDENAPVFLDRLDIYMDMDQPLSHYYINSSHNTYLSGRQFGGKSSVEMYRQTLLAGCRCVELDCWDGKGEDEEPIITHGMAMCTDILFKDVIYALRDTAFVTSDYPVILSFENHCCKSQQYKLAKYCDEILGDLLLKEPIPDYPLEPGAVLPPPSLLKRKILIKNKRLKPEVEKKELELFLQGEFVIEDEVKEDASAVDVTKIAELEAPAAAAAVVPVAAAAASQDGGEEAPPVQYTGSTTNVHPWLSSMVNYAQPVKFQSFDYADKKNVHHNMSSFAETTGMNLLKSQAIEFVNYNKRQMSRIYPKGTRADSSNYMPQVFWNAGCQMVSLNFQTSDLPMQLNQGKFEYNGNCGYLLKPDFMRRADRSFDPFADAPVDGVIAASCAVQVIAGQFLSDKKVGTYVEVDMYGLPSDTVRKEFRTRMVPANGLNPVYNEEPFLFRKVVLPDLAVLRFGVYDENGKLLGQRILPLDGLQGGYRHISLRTEANFPMSLPMLFCNIELKIYVPDGFEDFMDALSDPRAFMGAAKERSDNMKAMGIEETGKKDKEEAARKEEQRVTEPPLVFDPITVDSLRQEKGFQKTAKKQQKELDAVKKKHAKERAGVQKQQNAAIERLVKGKSKEEIKADPAVRKLIQEQNAQWTEMAERHKKEEWELLKQQLADQQDILRKLMETTQAAQMKQLEAKHEREIKELNSRQAKISVETSKEVANDKTLKTKGEKDRRLREKKQNNIKRFMDEKKTATIKQNREKEKLKVTHDKQLEELANDVQKLIEMYKVDEEEYKLASKYEFFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VEY0</accession>
    <name>A0A6E8VEY0_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Mitochondrial ribonuclease P catalytic subunit</fullName>
            <ecNumber evidence="5">3.1.26.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Mitochondrial ribonuclease P protein 3</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.</text>
            <dbReference type="EC" id="3.1.26.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.5" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8VEY0"/>
    <dbReference type="EnsemblMetazoa" id="ACON001968-RA">
        <property type="protein sequence ID" value="ACON001968-PA"/>
        <property type="gene ID" value="ACON001968"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011279"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001968"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038337"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030678">
        <property type="term" value="C:mitochondrial ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097745">
        <property type="term" value="P:mitochondrial tRNA 5'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18718">
        <property type="entry name" value="PIN_PRORP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11980:FF:000006">
        <property type="entry name" value="AGAP001968-PB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11980">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033495">
        <property type="entry name" value="MRPP3_PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031595">
        <property type="entry name" value="PRORP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13547">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13547:SF1">
        <property type="entry name" value="MITOCHONDRIAL RIBONUCLEASE P CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16953">
        <property type="entry name" value="PRORP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="6" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PRORP" evidence="16">
        <location>
            <begin position="275"/>
            <end position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00044536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00044559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF16953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001968-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="515" mass="59751" checksum="5AF9F56EA7228461" modified="2020-08-12" version="1">MFSRFNVLWLRRFAFPENVRYYAGVHQKNPQLKPATATVETIKAKLASSSTPTAEEWKSIRLNILNERRFNITNVDSVVLGLCPNLRMGKSYIAFLHQQGLQTNLAIVGKLLRLYRLKEGKLTEEDESDIWRMYSELREQNAILDANTCEHAINALTLTKHWKHCLELLEMIKISGTPDSSSYNCIATKAFQSGDESTGWIMMQEMCENKRLPNDDSFLAWLDYSRKREDQPFSANLERMLQFTNDRSVFLSKRIGKQLLELPSEFGIRVQETRITDGGKCSSCKGTLSSIVVSEDMFQSLRDKFLTAVLINKEIFNRTTPEELNRFQAFLAKTLPYDVVIDGLNVAFSSGNQKSPIVYAQQVAAVVRHYVRQKKRVLVIGRKHMDKWRSREMKYIRENSFLFLTDDLSQDDPFLLYAALESGPKTDFFSRDLMRKHSFMLGNELSGVFKRWQQEHQYSLLSIMPDGRVLIKAPFKYELYAHKSCDNRWHVPLVELGYRIPKVEKQNDWLCLSIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VF93</accession>
    <name>A0A6E8VF93_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Defective in cullin neddylation protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Neddylation of cullins play an essential role in the regulation of SCF-type complexes activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_040220324.1">
        <property type="nucleotide sequence ID" value="XM_040364390.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VF93"/>
    <dbReference type="EnsemblMetazoa" id="ACON002513-RB">
        <property type="protein sequence ID" value="ACON002513-PB"/>
        <property type="gene ID" value="ACON002513"/>
    </dbReference>
    <dbReference type="GeneID" id="120948255"/>
    <dbReference type="CTD" id="40231"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001940"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002513"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038368"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097602">
        <property type="term" value="F:cullin family protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031624">
        <property type="term" value="F:ubiquitin conjugating enzyme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032182">
        <property type="term" value="F:ubiquitin-like protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045116">
        <property type="term" value="P:protein neddylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000041">
        <property type="entry name" value="DCN1-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.200:FF:000002">
        <property type="entry name" value="DCN1-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.200">
        <property type="entry name" value="Cullin, PONY binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014764">
        <property type="entry name" value="DCN-prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042460">
        <property type="entry name" value="DCN1-like_PONY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005176">
        <property type="entry name" value="PONY_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12281:SF12">
        <property type="entry name" value="DEFECTIVE IN CULLIN NEDDYLATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12281">
        <property type="entry name" value="RP42 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03556">
        <property type="entry name" value="Cullin_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51229">
        <property type="entry name" value="DCUN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DCUN1" evidence="4">
        <location>
            <begin position="16"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU410713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002513-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="213" mass="24924" checksum="32F2EF1B1BEE3962" modified="2020-08-12" version="1">MGDGISRRYSKSEDAFNQKRCLTWFREYTTPDDPDTLGPEGMEKFCEDIGVEPENVAMLVLAYKMGAKQMGFFTQSEWLKGLTDLQCDTASKVQCKLEYLRSMLNDPNSFKIIYRYAYDFARDKDQRSMDIETAKAMLQLLLGKHWPLYAQFAQFLEQSKYKVINKDQWCNILEFSRTISNDLTNYDVDGAWPVMLDEFVEWLRQLRAQSTIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VF99</accession>
    <name>A0A6E8VF99_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Arb2 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VF99"/>
    <dbReference type="EnsemblMetazoa" id="ACON002541-RA">
        <property type="protein sequence ID" value="ACON002541-PA"/>
        <property type="gene ID" value="ACON002541"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON002541-RB">
        <property type="protein sequence ID" value="ACON002541-PB"/>
        <property type="gene ID" value="ACON002541"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001516"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002541"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040021"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035197">
        <property type="term" value="F:siRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031048">
        <property type="term" value="P:regulatory ncRNA-mediated heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000257">
        <property type="entry name" value="Uncharacterized protein, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048263">
        <property type="entry name" value="Arb2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053858">
        <property type="entry name" value="Arb2_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21357">
        <property type="entry name" value="FAM172 FAMILY PROTEIN HOMOLOG CG10038"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21357:SF4">
        <property type="entry name" value="FAM172 FAMILY PROTEIN HOMOLOG CG10038"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22749">
        <property type="entry name" value="Arb2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arb2" evidence="1">
        <location>
            <begin position="58"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF22749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002541-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="366" mass="41033" checksum="46FF9A2B99A277D6" modified="2020-08-12" version="1">MRVLLRLFQQQQHAKLLAAPSGGLFRWRQATAAAAAPVTAALVGISGIRMAASEERTYPTTLEGFGYGFNEEGKLRQLDKDTGKLTDRVFDYQVYTSLSENQAHYEALGDVITQYVYERLEEKEGLKKLYVPADVPPEEATFVFSTVEKLDRPKKLLVLIHGSGVVRAGQWARSLIINDCLDSGTQIPYIRRGRELGYEVLLLNTNDNYRTVKGARKPIRGSRNPTEHAVTVLEQYILASRPESVAIVAHSYGGVVTVELAQRFPDFFRDKVFAVGFTDSVHASSLVPEALKKIGRNWVTAKDPLDTPLTITKHDIPRHSAGHIKHEMTSYSCMSALFEFLESRYAEFRTNRGEEPRSKKAKGDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VHA4</accession>
    <name>A0A6E8VHA4_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="8">DM10 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VHA4"/>
    <dbReference type="EnsemblMetazoa" id="ACON003805-RA">
        <property type="protein sequence ID" value="ACON003805-PA"/>
        <property type="gene ID" value="ACON003805"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004830"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003805"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039905"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043014">
        <property type="term" value="F:alpha-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060285">
        <property type="term" value="P:cilium-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000281">
        <property type="term" value="P:mitotic cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.170:FF:000002">
        <property type="entry name" value="EF-hand domain (C-terminal) containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.170">
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="DM10_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040193">
        <property type="entry name" value="EFHC1/EFHC2/EFHB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12086">
        <property type="entry name" value="EF-HAND DOMAIN C-TERMINAL CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12086:SF9">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06565">
        <property type="entry name" value="DM10_dom"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00676">
        <property type="entry name" value="DM10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51336">
        <property type="entry name" value="DM10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0966">Cell projection</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="DM10" evidence="7">
        <location>
            <begin position="4"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="DM10" evidence="7">
        <location>
            <begin position="186"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="347"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="409"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="353"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003805-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="438" mass="49649" checksum="18DF0BA3239945C1" modified="2020-08-12" version="1">MNFFDKILKFTNPIFDCVLDERDREGGELMTYKLFYYLEDDTVSIKELKENQEGRDYFPMLLRKQKLPKNWKENPVSHPSIFLEKSDAEVSEYYSPKDLIVGTTIFVYGRKFLLLDCDGFTRCYYEKALKLTQPDRVRLDSSPKRAPRLEVPSYLGLGTPEDSLASYHSLVPKSPKKDVITYLVNVNKFLRYGCVLDTAHPEDKIRKFVLSLSLADGTITIMESSVDNSGIRGGRFLSPRKVWLPGCNPDEPEYYTAKDLHIGATVVVFAHRFKIVSADLYVYRYMQAYPEIFSPIAIDSVRNFLLAEGHLKDDLRRATEEEYQKLEQSDGPSEQGEVQKRLAGFQIGDSNGASPAPIASPRASPVPYGDAQAAPFAEPPEHPCPHPIIPDEELRKAYHTNEPDELAIQAYNVPEDPTSSRQGSPKKGSKSVHFQDDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VHA6</accession>
    <name>A0A6E8VHA6_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PHB domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the band 7/mec-2 family. Flotillin subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040227832.1">
        <property type="nucleotide sequence ID" value="XM_040371898.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040227833.1">
        <property type="nucleotide sequence ID" value="XM_040371899.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040227835.1">
        <property type="nucleotide sequence ID" value="XM_040371901.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VHA6"/>
    <dbReference type="EnsemblMetazoa" id="ACON003789-RB">
        <property type="protein sequence ID" value="ACON003789-PB"/>
        <property type="gene ID" value="ACON003789"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON003789-RC">
        <property type="protein sequence ID" value="ACON003789-PC"/>
        <property type="gene ID" value="ACON003789"/>
    </dbReference>
    <dbReference type="GeneID" id="120952522"/>
    <dbReference type="CTD" id="32425"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011465"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003789"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035915"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016600">
        <property type="term" value="C:flotillin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002020">
        <property type="term" value="F:protease binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045661">
        <property type="term" value="P:regulation of myoblast differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027705">
        <property type="entry name" value="Flotillin_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13806:SF46">
        <property type="entry name" value="FLOTILLIN-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13806">
        <property type="entry name" value="FLOTILLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="Band 7/SPFH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU366054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003789-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="281" mass="30920" checksum="5656320371C92690" modified="2020-08-12" version="1">MGIEILSFTIKDVYDDVQYLQSLGKAQTASVKRDADAGVAEANRDAGIREAECEKSAMDVKYSTDTKIEDNARMYKLQKANFDQEINTAKAESQLAYELQAAKIRQRIRNEEIQIDIVERRKQIEIETQEINRKDCELNATVKLPAEAESYRVQAIAEGKRTQTVESARAEAERIKKIGSAEAYAIEQVGKAEAERMRMKANVYKMYGDAAIMNIVLESLPKIAAEVAAPLAKTEEIVLIGGNDQTTGDVTRLVGQLPPAINALTGVDLSKVLGKIPGAKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VJB2</accession>
    <name>A0A6E8VJB2_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ENTH domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VJB2"/>
    <dbReference type="EnsemblMetazoa" id="ACON004504-RB">
        <property type="protein sequence ID" value="ACON004504-PB"/>
        <property type="gene ID" value="ACON004504"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000055"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004504"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040622"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030125">
        <property type="term" value="C:clathrin vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16989">
        <property type="entry name" value="ENTH_EpsinR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000006">
        <property type="entry name" value="Clathrin interactor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12276:SF45">
        <property type="entry name" value="CLATHRIN INTERACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12276">
        <property type="entry name" value="EPSIN/ENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01417">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00273">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50942">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENTH" evidence="2">
        <location>
            <begin position="19"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="200"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="547"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="200"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="215"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="229"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="253"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="547"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="565"/>
            <end position="578"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004504-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="656" mass="69760" checksum="FC7A7E278E9DDA0A" modified="2023-06-28" version="2">MDKFISMWKVRELADKVTNVVMNYTEIEGKVREATNDEPWGPTGPLMQELAHATFTYEHFPEVMSMLWKRMLQDNKTNWRRTYKSLLLLNYLVRNGSERVVTSSREHIYDLRSLENYTFVDENGKDQGINVRHKVRELIDFIQDDDRLREERKKAKKNKDKYIGMSSEAMGMRYGGSSGGAGSGGGMDYGGYRDSYDRRSEDRYNETRDHHEYDYQYEGEREDSDDESNGPHDRSNRYQDREPSKSPATRQNSSLSTGSTSAFGGGGGTGGSERKINLNIKPTGSSSTGSSLRSAQKTTKKIDLGAASGFAKTAATASAASASATPGDQFGINSPTHRNTHAEEIVGTGGKQQDIIDDLFKTCPTKSASTTDALVDEDDFNPRATEDFGDFESAFGASTGKPVLTTTTKGDDFADFAAFGKEPTQPAAAAPSTEASTDLLFGLSVGNTSTGASNNGGAQIDILSDLSGLSLGSNSNATLYPSMNNGSGGLLQPTPLLAANASNNNNNHSLLSGQGKQQQPATATNVGTTWKNAGNINIDLDNLLGNGAKGSRGGSGGGAGGSNAPSMNQLKSIHSSPVHQPMAAALSPQPPYGAGGMAPTPINLMMTPIQQQQQQPPPVGMMMGGIGGGAFLNGNSNNAASTPTGGASFGSFNAFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VJB9</accession>
    <name>A0A6E8VJB9_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">CLASP N-terminal domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_040221552.2">
        <property type="nucleotide sequence ID" value="XM_040365618.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VJB9"/>
    <dbReference type="EnsemblMetazoa" id="ACON004312-RB">
        <property type="protein sequence ID" value="ACON004312-PB"/>
        <property type="gene ID" value="ACON004312"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON004312-RC">
        <property type="protein sequence ID" value="ACON004312-PC"/>
        <property type="gene ID" value="ACON004312"/>
    </dbReference>
    <dbReference type="GeneID" id="120948846"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005041"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004312"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030214"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="238"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="363"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="409"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="366"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="409"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="440"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004312-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="609" mass="69067" checksum="5610C442A30572FA" modified="2020-08-12" version="1">MSENGFTQILTSGCLYSDTDSMADGVRRSRRRAYQRHEEEHEPTLMDESKEFPQYETTSGGDVRHMLAERGGACRRFETTVTSNDRDWNVLDRYNDEQDDRHDRERWRMGQPFKHERNRYGSSRSPGVPAVQLKVAHQEPISIRPRNKNPQIVNVCQTFRFPDNFITQNNATRMVQHQDRVVHQQHPAPEPRRPPLLSPCHSTSHTPVGGFFSMYRRHRNMHSQTSRTLIELVEAEQKMQQQQQTVRSTMGPRGTSNYGASSHRLAEDSLLASSGSETSLDELSDVRPRLLQGERKAKIPPMVREQLRAANGGRTSQVPYFDRTKRNRSYTKLVDGGATMGGHYGRSTKLPALGAVGKFSRYPTKSKADNRRPTLEEDALESPFVDDTFAPTGGRSRKLHLVDDALDDEEGLDEVNDESDVESSDEQQNNDRDECNGQLDLDDENEEDNDEESVGDSFGRRLGHGTEAVRAGTGTKEGPVSISGIVDKLQDNVWEVCLEGIWDLMDTASRIDWKAQEKYITVINRKLIEFLKSPRTALCRSACQVSGELFRQAKSTKRPEFDEMVDILLCKTADPNRFIQKDANVALDKLVTYIPTPHTVRAISNRGTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VJD6</accession>
    <name>A0A6E8VJD6_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Filamin-A</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the filamin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VJD6"/>
    <dbReference type="EnsemblMetazoa" id="ACOM040976-RA">
        <property type="protein sequence ID" value="ACOM040976-PA.1"/>
        <property type="gene ID" value="ACOM040976"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON004335-RA">
        <property type="protein sequence ID" value="ACON004335-PA"/>
        <property type="gene ID" value="ACON004335"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012661"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004335"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038938"/>
    <dbReference type="Proteomes" id="UP000075882">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000140">
        <property type="entry name" value="FiLamiN (Actin binding protein) homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000092">
        <property type="entry name" value="Filamin-B isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000007">
        <property type="entry name" value="Filamin-B isoform C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000096">
        <property type="entry name" value="filamin-C isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044801">
        <property type="entry name" value="Filamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017868">
        <property type="entry name" value="Filamin/ABP280_repeat-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001298">
        <property type="entry name" value="Filamin/ABP280_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38537:SF8">
        <property type="entry name" value="FILAMIN-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38537">
        <property type="entry name" value="JITTERBUG, ISOFORM N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00630">
        <property type="entry name" value="Filamin"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00557">
        <property type="entry name" value="IG_FLMN"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50194">
        <property type="entry name" value="FILAMIN_REPEAT"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="76"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="131"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="221"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="314"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="404"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="507"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="601"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="696"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="824"/>
            <end position="918"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004335-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="918" mass="99840" checksum="824CC97E65D3E898" modified="2020-08-12" version="1">MWSDDEEQGTEPVEATWEEHSQFYRSFSRFMSVSEHDRREMVRVFFIEQDVPVLESSDIDFVPTNMLSGFFLHTHKAEVKMPSGEVDKPVIDDNHDGTVSIRYEPKEEGIHELAVKYNGEHVQGSPFKFHVDSISSGYVTAYGPGLVHGVTGEPAQFIISTKGAGAGGLQMAVEGPSKADITYHDNKDGTVSVSYLPTAPGEYKISVRFGDKHIKGSPYFAKITGEGRKRNQISVGSCSEVTLPGVISDNDLRSLNASIQAPSGLEEPCFLKRMPTGNIGISFTPREIGEHTVSVKRLGKHIANSPFKVNVCEREVGDAKKVIVTGNALKEGKTHQDNVFSIDTRNAGYGGLSLSIEGPSKAEIQCTDKDDGTLNIAYKPTEPGYYIVNLKFADHHVTGSPFTVKVSGEGTNRQREKIQRQREAVPITEVGSQCKLTFKMPGITSFDLSATVTSPGGVSEDAEIQEIEDGLYAVHFIPKELGVHTVSVKYKQIHIPGSPFQFTVGPLKDTGAHLVKAGGPGLEHGEQGVPAEFNVWTREAGGGTLAISVEGPSKAEIEFKDRKDGSCDVSYVVSEPGDYRIGLKFNDRHIPDSPFKVYISPAMGEAHKLEVAQFPTGCVQADKPAQFMVRKNGAKGELDAKVVAPSNNEDDCFIQLIDQDQYSVRFYPRENGIHAIHVKFNGVHIPGSPYRIKVGKDDVDPAAVHASGKGLGDVKTGEKTDLIIDTCNAGAGTLAVTVDGPAKVAMDCTEVEEGYKVRYTPLLPGHYYMTIKYNQMHIVGSPFKINCTGESLAEAGGQETSSVIVETVAKVSKGGNRTGVILPIFKSDASKIQSKGMGLKKAYMGKQNQFTINAGDAGNNILFVGIYGPKGPCDEVFIKHTGRNQYTVNYLVRERGDYILLVKWGDDHIPGSPFKVEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VJE8</accession>
    <name>A0A6E8VJE8_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">EamA domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VJE8"/>
    <dbReference type="EnsemblMetazoa" id="ACON004544-RA">
        <property type="protein sequence ID" value="ACON004544-PA"/>
        <property type="gene ID" value="ACON004544"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006443"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004544"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041060"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026505">
        <property type="entry name" value="Solute_c_fam_35_mem_F3/F4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19346">
        <property type="entry name" value="SUGAR PHOSPHATE TRANSPORTER DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19346:SF4">
        <property type="entry name" value="SUGAR PHOSPHATE TRANSPORTER DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="237"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="288"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="319"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="344"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="374"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="403"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="438"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="468"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="494"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004544-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="543" mass="57900" checksum="D5C63D2C77415F40" modified="2023-06-28" version="2">MVENAMTRDSEIPAIFNPKRVRAPSVVVTGDSPNGPFGGFTNNVPQITHSDSVTSSQHDGQDSLIGITVTGGMHGGCDTPTSQPHALLGSGGGLGVGSGGGSGSANPAVSSSLPSTSRVKACRASCCSELAQKMYFGVCVTVLVTASWVGATHCIKFLYLRRNTYASTLPPHLTAFITDGTTDAIPGGAAPDQNSTSAMIVHGFGPTSSFLPSTTKPIITSDRVAPPLTTHVFNAPFFASWFCTNFAILFFPIYILGRVAIKKCDGTGEVLGEILRGFRDRGFTIGRFLNRCLTFCVLWLLTTYLYALSLKDLLATDVMALFATNVACVYLLAWVILQEQFVGVRIVAVILCDTGIALLAYMDGITGSPTLGGVVLAALAAAGYAVFKVMFRKVMGDPPVGQIAFTFSIIGFLNAAILWPVCIALYFTGAEIMPWETLPWVVLLMASVLLLVFHILTQFSSAVTYNMFVTLGLITAVPVSAALDIVLYGAHFAGMKLAGIILIAVGFFLVMFPDNWPDYITRLLRNIILLGHNARYTTKEVNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VJG0</accession>
    <name>A0A6E8VJG0_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glutamate dehydrogenase [NAD(P)(+)]</fullName>
            <ecNumber evidence="3">1.4.1.3</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-glutamate + NAD(+) + H2O = 2-oxoglutarate + NH4(+) + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15133"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.4.1.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-glutamate + NADP(+) + H2O = 2-oxoglutarate + NH4(+) + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11612"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.4.1.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Glu/Leu/Phe/Val dehydrogenases family.</text>
    </comment>
    <dbReference type="EC" id="1.4.1.3" evidence="3"/>
    <dbReference type="RefSeq" id="XP_049462672.1">
        <property type="nucleotide sequence ID" value="XM_049606715.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VJG0"/>
    <dbReference type="EnsemblMetazoa" id="ACON004362-RF">
        <property type="protein sequence ID" value="ACON004362-PF"/>
        <property type="gene ID" value="ACON004362"/>
    </dbReference>
    <dbReference type="GeneID" id="120961082"/>
    <dbReference type="CTD" id="42832"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004833"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004362"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040704"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004352">
        <property type="term" value="F:glutamate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006538">
        <property type="term" value="P:L-glutamate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01076">
        <property type="entry name" value="NAD_bind_1_Glu_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10860:FF:000007">
        <property type="entry name" value="Glutamate dehydrogenase 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000100">
        <property type="entry name" value="Glutamate dehydrogenase 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006095">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006096">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006097">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033524">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033922">
        <property type="entry name" value="NAD_bind_Glu_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11606">
        <property type="entry name" value="GLUTAMATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11606:SF13">
        <property type="entry name" value="GLUTAMATE DEHYDROGENASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00208">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02812">
        <property type="entry name" value="ELFV_dehydrog_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00082">
        <property type="entry name" value="GLFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00839">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00074">
        <property type="entry name" value="GLFV_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamate/phenylalanine/leucine/valine/L-tryptophan dehydrogenase C-terminal" evidence="9">
        <location>
            <begin position="261"/>
            <end position="551"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004362-PF"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="554" mass="61302" checksum="3DB9D13719612491" modified="2020-08-12" version="1">MYHLKTIAKNATIKQEVATLVKAVAAVQQPMAMNQQQPKRTYAEHQIPERLKDVSTAANPRFFDMVEYFFHRACQICEDKLVEDMKGRASMDEKKKKVKGILMLMQPCDHIIEIAFPLRRDSGDYEMITGYRAQHCTHRTPTKGGIRFSLDVSRDEVKALSALMTFKCACVDVPFGGAKAGVKIDPRKYSEHELEKITRRFALELSKKGFIGPGIDVPAPDMGTGEREMSWIADTYAKTIGHLDINAHACVTGKPINQGGIHGRVSATGRGVFHGLDNFIKEANYMAQIGTTPGWGGKTFIVQGFGNVGLHSCRYLTRAGATCIGVIEHDGSIFNPQGIDPKALEDYKNEKGTIVGFPGAQPYEGENLMYEQCDIFIPAAVEKVITAENAGKINAKIIAEAANGPTTPAADKILIDRNILVIPDLYINAGGVTVSFFEWLKNLNHVSYGRLTFKYERESNYHLLESVQASLERRFGNVGGKIPVTPSEAFQKRISGASEKDIVHSGLDYTMERSARAIMKTAMKYNLGLDLRSAAYVNSIEKIFQTYRDAGLAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VLE9</accession>
    <name>A0A6E8VLE9_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Peptidoglycan recognition protein family domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VLE9"/>
    <dbReference type="EnsemblMetazoa" id="ACON005203-RC">
        <property type="protein sequence ID" value="ACON005203-PC"/>
        <property type="gene ID" value="ACON005203"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_014474"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005203"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033832"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008745">
        <property type="term" value="F:N-acetylmuramoyl-L-alanine amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009253">
        <property type="term" value="P:peptidoglycan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06583">
        <property type="entry name" value="PGRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.80.10:FF:000001">
        <property type="entry name" value="Peptidoglycan recognition protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.80.10">
        <property type="entry name" value="Peptidoglycan recognition protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036505">
        <property type="entry name" value="Amidase/PGRP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002502">
        <property type="entry name" value="Amidase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015510">
        <property type="entry name" value="PGRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006619">
        <property type="entry name" value="PGRP_domain_met/bac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11022">
        <property type="entry name" value="PEPTIDOGLYCAN RECOGNITION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11022:SF41">
        <property type="entry name" value="PEPTIDOGLYCAN-RECOGNITION PROTEIN LC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01510">
        <property type="entry name" value="Amidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00644">
        <property type="entry name" value="Ami_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00701">
        <property type="entry name" value="PGRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55846">
        <property type="entry name" value="N-acetylmuramoyl-L-alanine amidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0391">Immunity</keyword>
    <keyword evidence="2" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="218"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidoglycan recognition protein family" evidence="7">
        <location>
            <begin position="280"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetylmuramoyl-L-alanine amidase" evidence="6">
        <location>
            <begin position="292"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="170"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="8"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="50"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="171"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005203-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="457" mass="49925" checksum="0D9005404F3E84F8" modified="2020-08-12" version="1">MALARNLQLENDTSVNTGYHTKAQSMSASGGDTIAGLNNSGTGTGKSDSAKGSSLSNISASSIKRTDNGGDDDDSSVFDSSSSDTECDDDSIKRAIDRIPGTLAPGEARVLPNANVKVAVEAIAPGVRPSPAASTIGAIAVHNSSDITFGNKTYIKGQVVIKNIYQDRKNGTTNQGYQVQENETDDTNSTKKVPSATKPPEPRTWQSSLKTIIKDKPLLSFIVMVSLMIVLCAIVAVISILTASGKARFRLPVGDGDDNRQNIPQDKDIEDDLFPDPRPLRLVTRTEWLAQPPREELTDLKLPVNNVIIAHTATEGCTTQAACRLRVRLIQEFHMDGKNYDDITYNFLIGGDGHIYEGRNWHKIGATIPGYNSRSITVAFVGEYNYGGKPTKKQIELLKYLLHFGAKERHLKEGYRIYASEQLDPTVGTGKWLIEALHSLPQFVDKEQNKDEQPDHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VLF4</accession>
    <name>A0A6E8VLF4_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Superoxide dismutase [Cu-Zn]</fullName>
            <ecNumber evidence="8">1.15.1.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Destroys radicals which are normally produced within the cells and which are toxic to biological systems.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 13">
            <text>2 superoxide + 2 H(+) = H2O2 + O2</text>
            <dbReference type="Rhea" id="RHEA:20696"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:18421"/>
            <dbReference type="EC" id="1.15.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
        <text evidence="8">Binds 1 copper ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the Cu-Zn superoxide dismutase family.</text>
    </comment>
    <dbReference type="EC" id="1.15.1.1" evidence="8"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8VLF4"/>
    <dbReference type="EnsemblMetazoa" id="ACOM037703-RA">
        <property type="protein sequence ID" value="ACOM037703-PA"/>
        <property type="gene ID" value="ACOM037703"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON005234-RB">
        <property type="protein sequence ID" value="ACON005234-PB"/>
        <property type="gene ID" value="ACON005234"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006252"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005234"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038832"/>
    <dbReference type="Proteomes" id="UP000075882">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004784">
        <property type="term" value="F:superoxide dismutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00305">
        <property type="entry name" value="Cu-Zn_Superoxide_Dismutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.200:FF:000001">
        <property type="entry name" value="Superoxide dismutase [Cu-Zn]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.200">
        <property type="entry name" value="Superoxide dismutase, copper/zinc binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036423">
        <property type="entry name" value="SOD-like_Cu/Zn_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024134">
        <property type="entry name" value="SOD_Cu/Zn_/chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018152">
        <property type="entry name" value="SOD_Cu/Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001424">
        <property type="entry name" value="SOD_Cu_Zn_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10003">
        <property type="entry name" value="SUPEROXIDE DISMUTASE CU-ZN -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00080">
        <property type="entry name" value="Sod_Cu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00068">
        <property type="entry name" value="CUZNDISMTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49329">
        <property type="entry name" value="Cu,Zn superoxide dismutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00087">
        <property type="entry name" value="SOD_CU_ZN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00332">
        <property type="entry name" value="SOD_CU_ZN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="6 8" id="KW-0186">Copper</keyword>
    <keyword evidence="2 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044631318" description="Superoxide dismutase [Cu-Zn]" evidence="9">
        <location>
            <begin position="17"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="Superoxide dismutase copper/zinc binding" evidence="10">
        <location>
            <begin position="33"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005234-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="171" mass="17691" checksum="A22F9EFB2BB24D0E" modified="2020-08-12" version="1">MKVLIALSTVLCVVLAKDQPRKAIVYLQGTSGVSGNVTISQPSCTEPVFIDINVVGLTPGKHGFHIHEKGDLTDGCASTGGHYNPDKVSHGAPNDQVRHVGDLGNIAADENGIAKTSYSDTVVSLYGARSVIGRAIVIHAEVDDLGKTNHPDSLKTGNAGGRVACGVIGIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VLG7</accession>
    <name>A0A6E8VLG7_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="17">BTB domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Putative transcription factor required for axon growth and guidance in the central and peripheral nervous systems. Repels CNS axons away from the midline by promoting the expression of the midline repellent sli and its receptor robo.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VLG7"/>
    <dbReference type="EnsemblMetazoa" id="ACON005245-RD">
        <property type="protein sequence ID" value="ACON005245-PD"/>
        <property type="gene ID" value="ACON005245"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002202"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009842"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005245"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_029613"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016199">
        <property type="term" value="P:axon midline choice point recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048813">
        <property type="term" value="P:dendrite morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008406">
        <property type="term" value="P:gonad development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035167">
        <property type="term" value="P:larval lymph gland hemopoiesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007526">
        <property type="term" value="P:larval somatic muscle development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045476">
        <property type="term" value="P:nurse cell apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007464">
        <property type="term" value="P:R3/R4 cell fate commitment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045467">
        <property type="term" value="P:R7 cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18315">
        <property type="entry name" value="BTB_POZ_BAB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000091">
        <property type="entry name" value="Lola, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000100">
        <property type="entry name" value="Zinc finger 45-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051095">
        <property type="entry name" value="Dros_DevTransReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110">
        <property type="entry name" value="BTB DOMAIN TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110:SF111">
        <property type="entry name" value="LONGITUDINALS LACKING PROTEIN, ISOFORMS F_I_K_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6" id="KW-0221">Differentiation</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="15">
        <location>
            <begin position="32"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="488"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="519"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="115"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="301"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="398"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="161"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="175"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="212"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="322"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="410"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="14">
        <location>
            <begin position="438"/>
            <end position="447"/>
        </location>
    </feature>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
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            <dbReference type="EnsemblMetazoa" id="ACON005245-PD"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="548" mass="57829" checksum="EB17F0456A8D5A62" modified="2020-08-12" version="1">MDDDQQFCLRWNNHQSTLISVFDTLLENGTLVDCTLAAEGKLLKAHKVVLSACSPYFATILSQQYDKHPIFILKDVKFQELRAMMDYMYRGEVNISQDQLAALLKAAESLQIKGLSDNRSSSSSGTAAAGGGVHQKQSDAPAAKTLQPPVPASKASGLTIENKRSLKSELLESDVSGSREGSTSPTSRKRKKVRRRSVDTNNLLIDNHDQHSNSSSHSMHTAGGANVAAAVAAAGAPAANVASSLSSASSTAAAGSNTSANVLSVAAVAAAAVLKKTETVQQAAAETLKLQKQQAAAAAAAAAAQSSDDEERCGPGSVATGTEHDDGNDLSDNEHDGDGGSSVKSGSRHHHLRDGSRRGKTSTSSMGEMMIEPKSEYEDVQDENVEDLTMDDEDLMDELDQAGPSHGGDVSSSTVVAPSSTTSAPSSSMAAGTVVSGGRRGGGGGSGSSLLDNSSGGSSRRSLLQLNDFLYDVKFSDLSKFLNAVGRYECPRRDCDKNYKDASSLQRHIRYECGGMKKFRCVMCGKAFSQGSHLKRHLESGVCIKYYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VLH0</accession>
    <name>A0A6E8VLH0_ANOCL</name>
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        <recommendedName>
            <fullName evidence="4">Transmembrane protein 223</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
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            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VLH0"/>
    <dbReference type="EnsemblMetazoa" id="ACON005148-RA">
        <property type="protein sequence ID" value="ACON005148-PA"/>
        <property type="gene ID" value="ACON005148"/>
    </dbReference>
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    <dbReference type="VEuPathDB" id="VectorBase:ACON005148"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Tmem223"/>
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        <property type="match status" value="1"/>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="12"/>
            <end position="33"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005148-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="171" mass="20046" checksum="19A631CF4E07FA80" modified="2020-08-12" version="1">MLFKYENPRFFKVLNIFAVSQFLFWGYLCHFSYTTLKDAPVQEDGKEDLPWYKRVNLGENKYRNGIAIMCFFIGYGVLFASWMFTLRSVRYLVLRKGGDKVSVVTYAPFGTNRIMDVPLKYVSAQESREAARVTVPVKIKNRALFYMLDMRGEFTNTKLYDYTVGLSRKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VLH2</accession>
    <name>A0A6E8VLH2_ANOCL</name>
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        <submittedName>
            <fullName evidence="13">BTB domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
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            <taxon>Neoptera</taxon>
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            <taxon>Nematocera</taxon>
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            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Putative transcription factor required for axon growth and guidance in the central and peripheral nervous systems. Repels CNS axons away from the midline by promoting the expression of the midline repellent sli and its receptor robo.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VLH2"/>
    <dbReference type="EnsemblMetazoa" id="ACON005245-RI">
        <property type="protein sequence ID" value="ACON005245-PI"/>
        <property type="gene ID" value="ACON005245"/>
    </dbReference>
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    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009842"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005245"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_029613"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016199">
        <property type="term" value="P:axon midline choice point recognition"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048813">
        <property type="term" value="P:dendrite morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008406">
        <property type="term" value="P:gonad development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035167">
        <property type="term" value="P:larval lymph gland hemopoiesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007526">
        <property type="term" value="P:larval somatic muscle development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045476">
        <property type="term" value="P:nurse cell apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007464">
        <property type="term" value="P:R3/R4 cell fate commitment"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045467">
        <property type="term" value="P:R7 cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="BTB_POZ_BAB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000091">
        <property type="entry name" value="Lola, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051095">
        <property type="entry name" value="Dros_DevTransReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110">
        <property type="entry name" value="BTB DOMAIN TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110:SF111">
        <property type="entry name" value="LONGITUDINALS LACKING PROTEIN, ISOFORMS F_I_K_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
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    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3" id="KW-0221">Differentiation</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="11">
        <location>
            <begin position="32"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="565"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="594"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="115"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="301"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="161"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="175"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="212"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="322"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005245-PI"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="619" mass="64519" checksum="CB6A3C36EFE06D39" modified="2020-08-12" version="1">MDDDQQFCLRWNNHQSTLISVFDTLLENGTLVDCTLAAEGKLLKAHKVVLSACSPYFATILSQQYDKHPIFILKDVKFQELRAMMDYMYRGEVNISQDQLAALLKAAESLQIKGLSDNRSSSSSGTAAAGGGVHQKQSDAPAAKTLQPPVPASKASGLTIENKRSLKSELLESDVSGSREGSTSPTSRKRKKVRRRSVDTNNLLIDNHDQHSNSSSHSMHTAGGANVAAAVAAAGAPAANVASSLSSASSTAAAGSNTSANVLSVAAVAAAAVLKKTETVQQAAAETLKLQKQQAAAAAAAAAAQSSDDEERCGPGSVATGTEHDDGNDLSDNEHDGDGGSSVKSGSRHHHLRDGSRRGKTSTSSMGEMMIEPKSEYEDVQDENVEDLTMDDEDLMDELDQAGPSHGGDVSISVPVGEASDTDGGPLVGGAAGGGAPEEGDLVTDPTGSEGSAAATVIAATAAAPQPQTTALPLLSPKSPPIDEQPCDLRMLTPATYLREFASAVKPESFAVAAATAIKLAASANTGSASTAAALPMPMFKGISMTTPPPQPAMAMPSPIVGGTYSCTRCGNSYARPHSLNRHIRFECGVEPKFECPVCHKKSKHKHNLVLHMRTHQHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VLH6</accession>
    <name>A0A6E8VLH6_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="7">SERPIN domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the serpin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VLH6"/>
    <dbReference type="EnsemblMetazoa" id="ACON005246-RB">
        <property type="protein sequence ID" value="ACON005246-PB"/>
        <property type="gene ID" value="ACON005246"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011585"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005246"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038865"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19601">
        <property type="entry name" value="serpin42Da-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.497.10:FF:000006">
        <property type="entry name" value="Plasminogen activator inhibitor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.39.10">
        <property type="entry name" value="Alpha-1-antitrypsin, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.497.10">
        <property type="entry name" value="Antithrombin, subunit I, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023795">
        <property type="entry name" value="Serpin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023796">
        <property type="entry name" value="Serpin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000215">
        <property type="entry name" value="Serpin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036186">
        <property type="entry name" value="Serpin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042178">
        <property type="entry name" value="Serpin_sf_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042185">
        <property type="entry name" value="Serpin_sf_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461:SF211">
        <property type="entry name" value="GH10112P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461">
        <property type="entry name" value="SERINE PROTEASE INHIBITOR, SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00079">
        <property type="entry name" value="Serpin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00093">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56574">
        <property type="entry name" value="Serpins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00284">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0722">Serine protease inhibitor</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Serpin" evidence="6">
        <location>
            <begin position="21"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005246-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="382" mass="42444" checksum="5B8BBEB2C6C9D26E" modified="2020-08-12" version="1">MADNSSSLDAQFVSQSNSFATKLYQRVSAKHAGENVVISPFSISACLSLAAMGAGGLTAEQMYSVLEFGAPDRKQTVADNYRRLMERLATDSTVNVANKIYVMQNYAVKGAFNAIATGSFRSEAESVNFAESAAAAKKINGWVEEKTNNKIKDLISPDALDELSRMVLVNAVHFKGTWTYQFDPSLTRPFPFWLSETESRDVPMMNIKKHFAFNNFEELGFSALELTYGGSDMTMMLLLPNERMGLAALEEKLPTLNLAELAGKMHKQEVEVFLPKFKIEFTRDLNEDLQALGMERMFSDSAEFPDLLEQNEPLKVSKVVHKAFIEVNEEGTEAAAATAMIAVSFCMIISEPVKFTVDHPFIYALMSPEKSVFFIGKTCKMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VLI2</accession>
    <name>A0A6E8VLI2_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Peptidoglycan recognition protein family domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VLI2"/>
    <dbReference type="EnsemblMetazoa" id="ACON005205-RA">
        <property type="protein sequence ID" value="ACON005205-PA"/>
        <property type="gene ID" value="ACON005205"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002089"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005205"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030932"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008745">
        <property type="term" value="F:N-acetylmuramoyl-L-alanine amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009253">
        <property type="term" value="P:peptidoglycan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06583">
        <property type="entry name" value="PGRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.80.10">
        <property type="entry name" value="Peptidoglycan recognition protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036505">
        <property type="entry name" value="Amidase/PGRP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002502">
        <property type="entry name" value="Amidase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015510">
        <property type="entry name" value="PGRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006619">
        <property type="entry name" value="PGRP_domain_met/bac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11022">
        <property type="entry name" value="PEPTIDOGLYCAN RECOGNITION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11022:SF76">
        <property type="entry name" value="PEPTIDOGLYCAN-RECOGNITION PROTEIN LA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01510">
        <property type="entry name" value="Amidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00644">
        <property type="entry name" value="Ami_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00701">
        <property type="entry name" value="PGRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55846">
        <property type="entry name" value="N-acetylmuramoyl-L-alanine amidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0391">Immunity</keyword>
    <keyword evidence="2" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="143"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidoglycan recognition protein family" evidence="7">
        <location>
            <begin position="197"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetylmuramoyl-L-alanine amidase" evidence="6">
        <location>
            <begin position="209"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="48"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="52"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005205-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="401" mass="43806" checksum="4F5686802940584B" modified="2020-08-12" version="1">MENASASTTGPVPDSVANALMSIHPRYNSFHQHNAFPAAAPVGSLGARYEQNADSPGMATNHQNGLSTGNGGNTTVQPAATSVINLSNSSDVVIGPMTQYQGSVTIYQYMDATVEASRIANGFNNQNQLMGPNTSPGIKYERLIVYSIITLFIIIGLVLLFYVIYMMFPSGDNIIPKELFFEDNSASKSIPNLGNGHMVIDRHNWGAQQGVHGPYKLPHPIPYVLITHIGVHSEICSDVHVCSIKMRTLQDAAIAEKSLQDIPSNFYVGGDGNVYVGRGWDTANAYANMSLAVCFMGDYGRYEPNDLQLSALDHLLTFGEKHHLLTEDYKIVAHRQARTTRSPGIKLYEKITKLTRWYPCGLPGYAKCGVEIGLPTVWDQEYPKSISTIVATVSDNSTQTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VLI5</accession>
    <name>A0A6E8VLI5_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">RHO1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rho family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_049462160.1">
        <property type="nucleotide sequence ID" value="XM_049606203.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_049462161.1">
        <property type="nucleotide sequence ID" value="XM_049606204.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_049462162.1">
        <property type="nucleotide sequence ID" value="XM_049606205.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VLI5"/>
    <dbReference type="EnsemblMetazoa" id="ACON005160-RC">
        <property type="protein sequence ID" value="ACON005160-PC"/>
        <property type="gene ID" value="ACON005160"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON005160-RD">
        <property type="protein sequence ID" value="ACON005160-PD"/>
        <property type="gene ID" value="ACON005160"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON005160-RE">
        <property type="protein sequence ID" value="ACON005160-PE"/>
        <property type="gene ID" value="ACON005160"/>
    </dbReference>
    <dbReference type="GeneID" id="120951008"/>
    <dbReference type="CTD" id="36775"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001560"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005160"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035242"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032154">
        <property type="term" value="C:cleavage furrow"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000915">
        <property type="term" value="P:actomyosin contractile ring assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001667">
        <property type="term" value="P:ameboidal-type cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022412">
        <property type="term" value="P:cellular process involved in reproduction in multicellular organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003006">
        <property type="term" value="P:developmental process involved in reproduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035099">
        <property type="term" value="P:hemocyte migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035006">
        <property type="term" value="P:melanization defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01870">
        <property type="entry name" value="RhoA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000095">
        <property type="entry name" value="Rho-related GTP-binding protein RhoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0636">Prenylation</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005160-PE"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="192" mass="21602" checksum="4DEDAB47A543EB1A" modified="2020-08-12" version="1">MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVADIEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSVDSPDSLENIPEKWTPEVKHFCPNVPIILVGNKKDLRNDPHTIKELAKMKQEPVKPQEGRAMAEKINAFAYLECSAKSKEGVREVFETATRAALQVKKKKKSKCVLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VLI8</accession>
    <name>A0A6E8VLI8_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="13">BTB domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Putative transcription factor required for axon growth and guidance in the central and peripheral nervous systems. Repels CNS axons away from the midline by promoting the expression of the midline repellent sli and its receptor robo.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VLI8"/>
    <dbReference type="EnsemblMetazoa" id="ACON005245-RA">
        <property type="protein sequence ID" value="ACON005245-PA"/>
        <property type="gene ID" value="ACON005245"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007393"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009842"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005245"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_029613"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016199">
        <property type="term" value="P:axon midline choice point recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048813">
        <property type="term" value="P:dendrite morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008406">
        <property type="term" value="P:gonad development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035167">
        <property type="term" value="P:larval lymph gland hemopoiesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007526">
        <property type="term" value="P:larval somatic muscle development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045476">
        <property type="term" value="P:nurse cell apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007464">
        <property type="term" value="P:R3/R4 cell fate commitment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045467">
        <property type="term" value="P:R7 cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18315">
        <property type="entry name" value="BTB_POZ_BAB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000091">
        <property type="entry name" value="Lola, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051095">
        <property type="entry name" value="Dros_DevTransReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110">
        <property type="entry name" value="BTB DOMAIN TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110:SF111">
        <property type="entry name" value="LONGITUDINALS LACKING PROTEIN, ISOFORMS F_I_K_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3" id="KW-0221">Differentiation</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="11">
        <location>
            <begin position="32"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="613"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="115"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="301"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="656"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="161"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="175"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="212"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="322"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005245-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="678" mass="70150" checksum="906343E41075851F" modified="2020-08-12" version="1">MDDDQQFCLRWNNHQSTLISVFDTLLENGTLVDCTLAAEGKLLKAHKVVLSACSPYFATILSQQYDKHPIFILKDVKFQELRAMMDYMYRGEVNISQDQLAALLKAAESLQIKGLSDNRSSSSSGTAAAGGGVHQKQSDAPAAKTLQPPVPASKASGLTIENKRSLKSELLESDVSGSREGSTSPTSRKRKKVRRRSVDTNNLLIDNHDQHSNSSSHSMHTAGGANVAAAVAAAGAPAANVASSLSSASSTAAAGSNTSANVLSVAAVAAAAVLKKTETVQQAAAETLKLQKQQAAAAAAAAAAQSSDDEERCGPGSVATGTEHDDGNDLSDNEHDGDGGSSVKSGSRHHHLRDGSRRGKTSTSSMGEMMIEPKSEYEDVQDENVEDLTMDDEDLMDELDQAGPSHGGDVSTLQIARPSAKPSCSGSMSHVTAIINPSTSSSSFSSSCIMSPDDCGFSDVCPRTSTRRLLNATLRAAAAVAAASAAASATKNSTSSNVLPCSPAATTALSSASDSFKATCCSSGDNAMCTENNSTIAASICGSNNSSNAGYGGAENSFNCEATVTATASKTTAAAAASASSSAFKCPRCPRAYGLSYTLERHMKYECGVAKQFGCFKCGKRFSRKDILKAHISNTRLHCAAAAVAAASVASAAAPSNATTSVVATDDDGEYHRKKEDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VLK3</accession>
    <name>A0A6E8VLK3_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Cytochrome b561 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">Ngousso</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_040219378.1">
        <property type="nucleotide sequence ID" value="XM_040363444.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VLK3"/>
    <dbReference type="EnsemblMetazoa" id="ACON005222-RB">
        <property type="protein sequence ID" value="ACON005222-PB"/>
        <property type="gene ID" value="ACON005222"/>
    </dbReference>
    <dbReference type="GeneID" id="120947770"/>
    <dbReference type="CTD" id="36395"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_013111"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005222"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039555"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1770:FF:000001">
        <property type="entry name" value="Cytochrome b reductase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043205">
        <property type="entry name" value="CYB561/CYBRD1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006593">
        <property type="entry name" value="Cyt_b561/ferric_Rdtase_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10106">
        <property type="entry name" value="CYTOCHROME B561-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10106:SF24">
        <property type="entry name" value="NO EXTENDED MEMORY, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03188">
        <property type="entry name" value="Cytochrom_B561"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="B561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50939">
        <property type="entry name" value="CYTOCHROME_B561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="4" id="KW-0349">Heme</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="28"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="97"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="142"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="177"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="219"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b561" evidence="12">
        <location>
            <begin position="34"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005222-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="256" mass="28805" checksum="015257FE8F1CA9F4" modified="2020-08-12" version="1">MPRNSTTTGGRYDDDDDRVWNCGAWFEYILVVVVSSILLIAASVLTIFWTIYYRKGFNMDDPKLQFNLHPVLMIGGYITLSGFSILLYRICRCCSHLIVKLCHTFFHACSIPCIVIGFMAVWDSHNQQQIPNFYSLHSWLGMITMGLFALQFVLGFFSFLILLCCENATYKFRSTMVPIHASFGVATFMLAIATAVTGLTQKAHFELGENYSQTVEEGIIMNSIGVILTGLGIIIPFAVRRSNSPANCKVYVTERI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VPN0</accession>
    <name>A0A6E8VPN0_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="10">MFS domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Spinster (TC 2.A.1.49) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VPN0"/>
    <dbReference type="EnsemblMetazoa" id="ACON006346-RC">
        <property type="protein sequence ID" value="ACON006346-PC"/>
        <property type="gene ID" value="ACON006346"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006697"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON006346"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040560"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17328">
        <property type="entry name" value="MFS_spinster_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044770">
        <property type="entry name" value="MFS_spinster-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23505:SF79">
        <property type="entry name" value="PROTEIN SPINSTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23505">
        <property type="entry name" value="SPINSTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="155"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="182"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="206"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="241"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="272"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="323"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="370"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="402"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="434"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="553"/>
            <end position="576"/>
        </location>
    </feature>
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        <location>
            <begin position="117"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="597"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="20"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="52"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON006346-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="618" mass="66293" checksum="45D0FB887F180AF8" modified="2020-08-12" version="1">MPSSQGYSKVASEPPIDPVDSSTADSSSMPAGGGNVGAAATAIMGKAPASGGIQSNPAGKIPATNSQQRLMPTDSDSVTSSMDEGELRVARGNPHDEDTDTVTTTTTSSIGANAWFTVGVLCFVNLINYMDRFTIAGVLTEIQDHFKIGDDEGGLLQTAFVLSYMVFAPLFGYLGDRYSRKWIMVLGVSLWSTTTLLGSYMDHFGWFITFRALVGIGEASYSTIAPTIISDLFVGEMRSRMLALFYFAIPVGSGLGYIVGAKMASIMNSWVWSLRVTPVLGAIAVVLIVMLRDPQRGQSEGTHHMQTTSYKEDVKAIMRNRSFMLSTAGFTCVAFVTGALAWWGPKFIHLGLISQPGNEHVTLNDVSYKFGTVAMVAGLIGVPLGTLLSSRLRPHYRNADPLICAAGLITSSPLIYLGLVVVKYSGGWCFTLVFFAEVALNLSWPIVADMLLYVVVPTRRSTAEAFQILISHAFGDAGSPYFVGVISESIKRVLRIVHDAVATDAYHPSVESSSSHSIAVLSQLAENSTVAMLQAASPPAEDSPLVQFRALQYALFSTCFVEILGGVFFLVTAFYIDRDRARVEAVVLGNQSNNASAHQLGGSVSDMEDALPPPSTRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VPN4</accession>
    <name>A0A6E8VPN4_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Cyclic nucleotide-binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cAMP-dependent kinase regulatory chain family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040218587.1">
        <property type="nucleotide sequence ID" value="XM_040362653.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VPN4"/>
    <dbReference type="EnsemblMetazoa" id="ACON006448-RC">
        <property type="protein sequence ID" value="ACON006448-PC"/>
        <property type="gene ID" value="ACON006448"/>
    </dbReference>
    <dbReference type="GeneID" id="120947380"/>
    <dbReference type="CTD" id="40305"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_008284"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON006448"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042399"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030552">
        <property type="term" value="F:cAMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004862">
        <property type="term" value="F:cAMP-dependent protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034236">
        <property type="term" value="F:protein kinase A catalytic subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007611">
        <property type="term" value="P:learning or memory"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000013">
        <property type="entry name" value="cAMP-dependent protein kinase type I regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000006">
        <property type="entry name" value="cAMP-dependent protein kinase type I-alpha regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050503">
        <property type="entry name" value="cAMP-dep_PK_reg_su-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012198">
        <property type="entry name" value="cAMP_dep_PK_reg_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE REGULATORY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635:SF152">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE TYPE I REGULATORY SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000548">
        <property type="entry name" value="PK_regulatory"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00103">
        <property type="entry name" value="CAMPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00888">
        <property type="entry name" value="CNMP_BINDING_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00889">
        <property type="entry name" value="CNMP_BINDING_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0114">cAMP</keyword>
    <keyword evidence="3 7" id="KW-0116">cAMP-binding</keyword>
    <keyword evidence="5 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="9">
        <location>
            <begin position="80"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="9">
        <location>
            <begin position="198"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="11"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>3',5'-cyclic AMP</name>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>3',5'-cyclic AMP</name>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="269"/>
        </location>
        <ligand>
            <name>3',5'-cyclic AMP</name>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="278"/>
        </location>
        <ligand>
            <name>3',5'-cyclic AMP</name>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000548-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON006448-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="324" mass="35991" checksum="242F30209DA88F77" modified="2020-08-12" version="1">MLTLPLEQAKLDASKQAVTPDDLDDLSPMPQTAVPPVRRRGGISAEPVTEEDATSYVKKVVPKDYKTMAALSKAIAKNVLFSHLDENERSDIFDAMFPCNFLPGEPIIQQGDEGDNFYVIDIGEVEVFVNNEQVTTISEGGSFGELALIYGTPRAATVRAKTDVKLWGIDRDSYRRILMGSTIRKRKMYEEFLSRVSILESLDKWERLTVADALEPVSFEDGETIVRQGEPGNDFYIIVEGCATVMQKRGDSEEAAEVGRLGPSDYFGEIALLLDRPRAATVIARGPLKCVKLDRARFERVLGLCADILKRNITQYNSFVSLSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VPP8</accession>
    <name>A0A6E8VPP8_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Mitogen-activated protein kinase kinase kinase dlk-1</fullName>
            <ecNumber evidence="2">2.7.11.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">DAP kinase-like kinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Death-associated protein kinase-like kinase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.25"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.25"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.25" evidence="2"/>
    <dbReference type="RefSeq" id="XP_040229235.2">
        <property type="nucleotide sequence ID" value="XM_040373301.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040229236.2">
        <property type="nucleotide sequence ID" value="XM_040373302.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_049462997.1">
        <property type="nucleotide sequence ID" value="XM_049607040.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_049462998.1">
        <property type="nucleotide sequence ID" value="XM_049607041.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VPP8"/>
    <dbReference type="EnsemblMetazoa" id="ACON006461-RA">
        <property type="protein sequence ID" value="ACON006461-PA"/>
        <property type="gene ID" value="ACON006461"/>
    </dbReference>
    <dbReference type="GeneID" id="120953409"/>
    <dbReference type="CTD" id="40143"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000017"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON006461"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_029614"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004709">
        <property type="term" value="F:MAP kinase kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000087">
        <property type="entry name" value="Mitogen-activated protein kinase kinase kinase 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF304">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 13-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="179"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="64"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="631"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="665"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="762"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="973"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1020"/>
            <end position="1063"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1079"/>
            <end position="1193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="71"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="85"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="669"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="771"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="783"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="829"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="973"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="984"/>
            <end position="997"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="1048"/>
            <end position="1059"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1087"/>
            <end position="1102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="1123"/>
            <end position="1137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="1140"/>
            <end position="1153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1175"/>
            <end position="1184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00074193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00077446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00080806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON006461-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1193" mass="131693" checksum="ECD98EF7A2D98B97" modified="2020-08-12" version="1">MVFVGSFFERCCEPPDKPVLENVIVKKGMISIQEELDQLGLNNEQQQPAPPYPQVIYGNEYHHHHHHQQQVGNDDSSSPDYLQHHLPHHHHQHHPAMCANGWPQLPVGMVAAGGPGVGPVGVGIGVGVGVGMSSKPATAGWMDGLFGCMRPVLSLIGKSHIMEMKSKQTEDWEIPYETITDMVWLGSGAQGAVFCGKLRNELVAVKKVRELKETDIRHLRKLDHENIVKFKGVCTQAPVFCIIMEYCAHGPLHKKLQDSGGVITPQQLVSWSQQIALGMQYLHTHKIIHRDLKSPNILIGENDVIKISDFGTSREWNEISTKMSFAGTVAWMAPEVIRNEPCNEKVDIWSYGVVLWELLTGEVPYKNVDSSQIIFGVGNNSLYLPIPDTCPEGFKLLIKQCWSAKPRNRPSFKIILTHLDIAGRELLQACEKGQYELYYQTQRSWREDIRSHMQKLTSNGMDIQKHEQDLIQKRKDEWKHAQDVRMTYERKLERTNMLCMQLSQLILQVEQKEQEILKREKLLAPEQRKCGFLKRGGDKANRKRPFSFPAMTTALPGRGGYGYSTTPSPTAAGVTPAKATLYAELNPSGHQGAKSVVVPVAAAPPPPYSTLAPLQTAAAAVPVTSPITSPEAVPVQPMAPSTTSSGRVKKLRHRRVGSGTINCSPKCSPCRDRRVQSEPESRHVKLVDTETQTEPMDISEPDVSPCPNLASKRMSASLREEEEEEQEKVEPEVIEEALRRLSVSERLAGDRVLPVETRVSAYRCSSKPQSEDDGEVPEDEENGNSISISTMTNSRSSDMMTTSGGVPVTGSEQNYRYRRQQSSQQQEDLREEEADGYVEEFDDEREGSSPDPIMDAMNRNERFDRACSDDDKIDTLDRKVNIISEKLQQSAAYGNLLNDNNVIVYRAALKQPNGPPSGGKTILLKHPGAGSNLKPAGAGGAGIGAYLYSGPHAGGLAVVGGGGGGAGAVPAEVEQHHEPKLGEQEEEESWTDEEGEEPDQKQYYVLRRKSVGRLPIKRGRRTKYSVGSGPSVASGGGGVGVSGSSAMVHHHHHHHHHHASPGGADHLAIVHRKIHSNVTISDEENTSEYSHAPSSQRSTLESNPDLPSVVMMKAGRRVDRIKQQQQQQPMHKSTTSAKDTDDDDDDEDEDADDDGHVPDSDVEQDSSDSSSSSDEVERVDRRPVADVVNGNRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VPQ5</accession>
    <name>A0A6E8VPQ5_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">2-oxoglutarate dehydrogenase complex component E1</fullName>
            <ecNumber evidence="6">1.2.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="24">2-oxoglutarate dehydrogenase complex component E1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20 22">2-oxoglutarate dehydrogenase, mitochondrial</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Alpha-ketoglutarate dehydrogenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">Thiamine diphosphate (ThDP)-dependent 2-oxoglutarate dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="27 28">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="27">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="19">The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[protein] + 2-oxoglutarate + H(+) = N(6)-[(R)-S(8)-succinyldihydrolipoyl]-L-lysyl-[protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:12188"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:20092"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83120"/>
            <dbReference type="EC" id="1.2.4.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:12189"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the alpha-ketoglutarate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.4.2" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8VPQ5"/>
    <dbReference type="EnsemblMetazoa" id="ACON006366-RC">
        <property type="protein sequence ID" value="ACON006366-PC"/>
        <property type="gene ID" value="ACON006366"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006013"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON006366"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033405"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045252">
        <property type="term" value="C:oxoglutarate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004591">
        <property type="term" value="F:oxoglutarate dehydrogenase (succinyl-transferring) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02016">
        <property type="entry name" value="TPP_E1_OGDC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12470:FF:000007">
        <property type="entry name" value="2-oxoglutarate dehydrogenase e1 mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000002">
        <property type="entry name" value="2-oxoglutarate dehydrogenase, E1 component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11610:FF:000003">
        <property type="entry name" value="2-oxoglutarate dehydrogenase, isoform X4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1150:FF:000001">
        <property type="entry name" value="2-oxoglutarate dehydrogenase, mitochondrial isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11610">
        <property type="entry name" value="Multifunctional 2-oxoglutarate metabolism enzyme, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1150">
        <property type="entry name" value="TPP helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032106">
        <property type="entry name" value="2-oxogl_dehyd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011603">
        <property type="entry name" value="2oxoglutarate_DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001017">
        <property type="entry name" value="DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042179">
        <property type="entry name" value="KGD_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031717">
        <property type="entry name" value="ODO-1/KGD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00239">
        <property type="entry name" value="2oxo_dh_E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006914">
        <property type="entry name" value="PRK09404.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008907">
        <property type="entry name" value="PRK12270.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23152:SF4">
        <property type="entry name" value="2-OXOADIPATE DEHYDROGENASE COMPLEX COMPONENT E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23152">
        <property type="entry name" value="2-OXOGLUTARATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16078">
        <property type="entry name" value="2-oxogl_dehyd_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00676">
        <property type="entry name" value="E1_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16870">
        <property type="entry name" value="OxoGdeHyase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000157">
        <property type="entry name" value="Oxoglu_dh_E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="16" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="7" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="12" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="11" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Transketolase-like pyrimidine-binding" evidence="26">
        <location>
            <begin position="656"/>
            <end position="869"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00030680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00037426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00040267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00040429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00041946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00042799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00042984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00051042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON006366-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1019" mass="114565" checksum="A93484BB19B8B932" modified="2020-08-12" version="1">MHRARTALHMVNPMGQQNFGSFLLKKQASKLTTELVAASSVKLYNSAAAEPFLNGSSSNYIDDMYNAWLRDPASVHASWDAYFRNNSYAAPPSLAPVPKNHVPAAQYLGSSLPAVAGAGAAVGGRIDDKLIDDHLAVQAIIRSYQSRGHLVADIDPLGILNAEINPERANLRANEKVTRSYMNFEEADMERVFKLPSTTFIGGKEKFLPLREILGRLEKAYCNKIGVEFMFINSLEQCNWIRERFETPNIMTYTNEEKRLILARLTRATGFEAFLAKKFSSEKRFGLEGCEIMIPAMKEVIDVSTRLGVESIIMGMPHRGRLNVLANVCRKPLHQIFTQFAGLEAADDGSGDVKYHLGTYIERLNRVTNKNIRLAVVANPSHLEAVDPVVQGKTRAEQFYRGDGEGKKVMSILLHGDAAFCGQGVVFETMHLSDLPDYTTHGTIHIVVNNQIGFTTDPRHSRSSPYCTDVARVVNAPIFHVNGDDPEAVMHVCKVAAEWRATFHKDVIIDIVSYRRNGHNEIDEPMFTQPLMYKKIRGTKPALDIYANQLITEGVVTAEEVKSVKDKYEKICEEAFEQAKIETHIKYKDWIDSPWSGFFEGKDPLKVAPTGVIEETLVHIGNRFSSPPPNAAEFVIHKGLLRVLAARKEMLENKTIDWALAEAMAFGSLLKEGIHVRLSGQDVERGTFSHRHHVLHHQTVDKATYRPLCHLYPDQAPYTVCNSSLSEFGVLGFELGYSMTNPNALVCWEAQFGDFNNTAQCIIDQFVSSGQAKWVRQSGLVMLLPHGMEGMGPEHSSARVERFLQMCSDDPDYFPPESEEFAIRQLHDINWIVANCSTPGNYFHLLRRQIALPFRKPLIVLTPKSLLRHPECRSNFSEMTDGTEFKRLIPDALTAENPNQVKRVIFCTGRVYYDLLKARRDRKLDHDIAISRIEQISPFPYDLVKAECAKYPNAELVWAQEEHKNQGCWTYVQPRFDTAINSTRDFSYVGRPCGASTATGSKAQHLKELKNLLDDAMAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VPQ9</accession>
    <name>A0A6E8VPQ9_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein O-GlcNAc transferase</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VPQ9"/>
    <dbReference type="EnsemblMetazoa" id="ACON006254-RC">
        <property type="protein sequence ID" value="ACON006254-PC"/>
        <property type="gene ID" value="ACON006254"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000731"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON006254"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033711"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097363">
        <property type="term" value="F:protein O-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000252">
        <property type="entry name" value="O-linked N-acetylglucosamine (GlcNAc) transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037919">
        <property type="entry name" value="OGT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44366">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE--PEPTIDE N-ACETYLGLUCOSAMINYLTRANSFERASE 110 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44366:SF1">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE--PEPTIDE N-ACETYLGLUCOSAMINYLTRANSFERASE 110 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13414">
        <property type="entry name" value="TPR_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="114"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="148"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON006254-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="218" mass="24286" checksum="609E751B8854269B" modified="2020-08-12" version="1">MQGQIPGAAAVQVQGGTPQAAALAAQQQQQQQSISVKMDPANQLSSAGLLELAHREYQAVDYDNAERHCMQLWRQESNNTGVLLLLSSIHFQCRRLDKSAQFSTLAIKQNPLLAEAYSNLGNVYKERGQLQEALENYRHAVRLKPDFIDGYINLAAALVAARDMEQAVQAYVTALQYNPDLYCVRSDLGNLLKALGRLDEAKHEMPHTHNFSLLYCMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VQ17</accession>
    <name>A0A6E8VQ17_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Gamma-aminobutyric acid receptor subunit beta</fullName>
        </recommendedName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="24">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="18">Postsynaptic cell membrane</location>
            <topology evidence="18">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040228040.1">
        <property type="nucleotide sequence ID" value="XM_040372106.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VQ17"/>
    <dbReference type="EnsemblMetazoa" id="ACON006028-RC">
        <property type="protein sequence ID" value="ACON006028-PC"/>
        <property type="gene ID" value="ACON006028"/>
    </dbReference>
    <dbReference type="GeneID" id="120952660"/>
    <dbReference type="CTD" id="39054"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011712"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON006028"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033065"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005230">
        <property type="term" value="F:extracellular ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004890">
        <property type="term" value="F:GABA-A receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099095">
        <property type="term" value="F:ligand-gated monoatomic anion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19008">
        <property type="entry name" value="LGIC_ECD_GABAR_RDL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19049">
        <property type="entry name" value="LGIC_TM_anion"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000021">
        <property type="entry name" value="Gamma-aminobutyric acid receptor isoform 3b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000033">
        <property type="entry name" value="Gamma-aminobutyric acid receptor subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006028">
        <property type="entry name" value="GABAA/Glycine_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002289">
        <property type="entry name" value="GABAAb_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00860">
        <property type="entry name" value="LIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01160">
        <property type="entry name" value="GABAARBETA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00253">
        <property type="entry name" value="GABAARECEPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-0868">Chloride</keyword>
    <keyword evidence="12" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="17 20" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 20" id="KW-0406">Ion transport</keyword>
    <keyword evidence="16" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="9 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 20" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0770">Synapse</keyword>
    <keyword evidence="4 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 20" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026370282" description="Gamma-aminobutyric acid receptor subunit beta" evidence="20">
        <location>
            <begin position="35"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="262"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="297"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="328"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="520"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="22">
        <location>
            <begin position="56"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="23">
        <location>
            <begin position="269"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="367"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="444"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="21">
        <location>
            <begin position="393"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="406"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="21">
        <location>
            <begin position="461"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00071250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON006028-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="555" mass="61281" checksum="163B7F1732735D8A" modified="2020-08-12" version="1">MSLTIEVPHAKSPSLGVLILTLNLALFLPQTINRTPPYVLAGTGGGSMLGDVNISAILDSFSVGYDKRVRPNYGGPPVEVGVTMYVLSISSVSEVLMDFTLDFYFRQFWTDPRLAYRKRPGVETLSVGSEFIKNIWVPDTFFVNEKQSYFHIATTSNEFIRIHHSGSITRSIRLTITASCPMNLQYFPMDRQLCHIEIESFGYTMRDIRYFWKDGLSSVGMSSEVELPQFRVLGHRQRATEINLTTGNYSRLACEIQFVRSMGYYLIQIYIPSGLIVIISWVSFWLNRNATPARVSLGVTTVLTMTTLMSSTNAALPKISYVKSIDVYLGTCFVMVFASLLEYASVGYMAKRIQMRKQRFMAIQKIAEQKKQQQADANHPPPPPPVASDHSHGHGHGHSHTHQHTPKQQMGSRSGTMQNVPPNMGSRGCSIVGPLFQEVRFKVHDPKAHSKGGTLENTINGGRGGGQPGGGGPPPGGPPGGGGGGPDEESAAPQHLIHPGKDINKLLGITPSDIDKYSRIVFPVCFVCFNLMYWIIYLHVSDVVADDLVLLGEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VQ46</accession>
    <name>A0A6E8VQ46_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Mediator of RNA polymerase II transcription subunit 13</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the Mediator complex, a coactivator involved in regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the Mediator complex subunit 13 family.</text>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON006436-RA">
        <property type="protein sequence ID" value="ACON006436-PA"/>
        <property type="gene ID" value="ACON006436"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_008376"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON006436"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031447"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009401">
        <property type="entry name" value="Med13_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051139">
        <property type="entry name" value="Mediator_complx_sub13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041285">
        <property type="entry name" value="MID_MedPIWI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48249">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48249:SF3">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06333">
        <property type="entry name" value="Med13_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18296">
        <property type="entry name" value="MID_MedPIWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0010">Activator</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0678">Repressor</keyword>
    <keyword evidence="7 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MID" evidence="12">
        <location>
            <begin position="2014"/>
            <end position="2331"/>
        </location>
    </feature>
    <feature type="domain" description="Mediator complex subunit Med13 C-terminal" evidence="11">
        <location>
            <begin position="2369"/>
            <end position="2781"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="240"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="372"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="416"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="537"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="635"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="965"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="998"/>
            <end position="1023"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1043"/>
            <end position="1071"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1087"/>
            <end position="1187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1200"/>
            <end position="1236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1280"/>
            <end position="1344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1390"/>
            <end position="1437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1527"/>
            <end position="1584"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1652"/>
            <end position="1672"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="2130"/>
            <end position="2242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="2625"/>
            <end position="2666"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="240"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="425"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="494"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="537"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="566"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="644"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="677"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="764"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="780"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="842"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="969"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="1005"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="1061"/>
            <end position="1070"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="1205"/>
            <end position="1215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1284"/>
            <end position="1312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1313"/>
            <end position="1324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="1325"/>
            <end position="1338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1398"/>
            <end position="1416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1653"/>
            <end position="1672"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="2131"/>
            <end position="2144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="2156"/>
            <end position="2178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="2190"/>
            <end position="2199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="2214"/>
            <end position="2223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="2625"/>
            <end position="2648"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF06333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF18296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON006436-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="2792" mass="292377" checksum="985D388A5A62914A" modified="2023-06-28" version="2">MTHQNHQTNGASLEDCHTNFFALTDLCGIRWRQLVLGERPNAAGDPLDDPVVRSYSKCLAVDILCVWRRVVAPKPKPKPDPDPSSMFDMSIPGTGNSGSVVHPPLSLTAAKELWIFWYGEEPDLTDLVAPELLNSSEAGDKGSWENGLSYECRSLLFKALHNVIERCLLSRGFVRIEQWFVHPTSNDERVFGKSSHHASFSFTFFVHGDSTVITAMDIREHPPVRLLTMKHLEEAQRAAAPGAAAAAASTTGGPASPDGGLNAAPGCAPASPGGSASGTVVPADDAIEPREVLLAPFAITGTLTGQSYRASDPHTQKILDDWSAFYPITNKHSGLADGADSMPPLVEVLVGKMKMRYPSKYVLVPDMDDWTSAGDARSNSSSSSSNSSNTNSNQTTTPATAAASIAASVLSRINSASERSGGGVASNSTTSNTINTTSNQTAAAGSSSSNAASAASSARPKDTANDAAAPSPTGTLRQRNHHKSMMTPPLTVPESPSNDLGNPLSMSSALINKSPAVILPERVWQDCIMHAPTPAATAASSSASSGTTATTTAPGSVTIKTEPKDNTSGSNSSTNNNNNNNNNNSNSSSGGGDENSSGAANGVDANGNSSVWEISDPTVKVSCCCIKSNQNCAANSGSGGGNSGASNTTGAAPGSTTPYGGPPSYCRNPMSIGDSHPLPSEGSPASAAPSPMQPSSVSQPTSVPSGDQLITMSPHPPASNNPRSVQPGTPCLDHLDKNTPAPTPTDQHDSKSINASPYHTPSHPGGGGGGGGSSGGTTEGGQQQQASANLMGNSSSSSSSNSSSNGSNSSSNSLSALKASPRSSLSSSGIGSLKVRSESSLGAGGSCSLGSGGGSGGGVPMTPSIHSVFSSAHQTALANNLASKRESILQMLQSLKRPSLFCKDYETLQGEDIPSTSQLLYDYTCVDAWMNHPVKRMRLAAEENRLVKKIRNLDLYANEHSRTMASGPGLAGSGNSTNGGIGTNGMLMKGAAEASEALAEALSGGRPGTGGGSDNGAPNNGAGCTPLLGQIKMEIGCDGSQGGTAVGSANGGTPDRNGASAGNGGAGDGALLGEHEIKKEKEDKGSIFLTDQDLQPSIDDLNQIFDDGGPFNDDPDLHNTPPGSNKSSSGSGGFSEESKRFPISNHHSVNSGMLSPKELSQMFPTPPSLDPHPNSSPGGGGCLSDGGSIALDTLVDLQMSLSSGTGSGGGNGGGASSVPPIPNLGSPQEEPIDDWSFVFLPPPICPYVGSSKYAPLPSLPSQQVMPPIPTSTVAGLVYKSTRQKQKEQAAAEREKQKQREQAEREREREQKQEQQQQDQPDGQPESGGQGGAGAGTGRPLGVAGPTTTALGVGLGLLPIVKQEVGGLTVPQPLSTPNSLLGGLSGLNGGSLAHPYSNPPSQGAQSQPHSRPPSVNSLPGGIMGGGAGMLSPSPAGTSGGGLFHPGGMGGIPVPLRQGMSPISPATPGGSMRVPTPQGSCPLPYPSPLGGAGSPNPMGSLSFRRTTPLLPPPPYDVAIASPANSVATPSSYHSKQYPLDGVDSGGPGSNRGPMTPSSTGGSGPLGAGTVHPPLSSSGVGCGTGTPAGSRSIETNALLVNVLLYDTALNIFRDHNFKSCTLCVCNAGAKCVGNIRGADSGLYLALPGTNWMDSVTAAGAEPTPSTTPGPAGGRSKASALAHLGLGGSSSAFGAGMLGSPAVGRQEDKSAAVTINTDSLQNGYLDEDPIDCQCGFSAVVNRRMAHRAGLFYEDEMEITGMAEDPAVHKNGSLFEFLNGTAKAIKLEDADRGALATSAGGSSSSAAGGDSLPLTLKVMDLLREQCSVVQSSSNSIHRAVNRYRGPLFGRGAENGAVHLLEYVDANDVISLALEQGRLQAINESKMDVDQQQQQQSSSGALVKGGTLRGQMNVHKWPFLRAGGPKSNQDIVRIMKSMQPLLQDAFHKRCTTRLWDAPYTIQGPLTWRQFHRLAGRGTGQCEPQPIPSLIVGYEKDWLSLAPYALHYWDKLLLEPYSYPRDVAYVAIVPDNDYVVAKVRTYFKELSTTYEMCKLGRHTPIKGWDGILRVGSQRVLKETQNSNLDEWFTNMGVDQQRKNGTTTAGSASSSSSSGLNELLRLYAQTCQQQLAPYLSKVPSDKSLLDPPESHHHTSSSASASASLGGGAAGASSLGGGSMGQPGGNAMGQNRPDGPGQSPMPPPPPSTPDSSQPGDKAPNTPKFDHDSENRDQLNTSSASPADGMRIDDDGKDPPHIVLYIVEPFTCGNDSPDVERLACLSLLRCYSNILNAVPDSIRSNISVQIISLESILELGRNRNRLRLSDHMRCLALSVFSQSRKYLAHMNTVKSLTGFGTAANAEQFIKRKDDKNRVPYRLYTPPYVLARSCEKSENTESFGKTSMKQQCSIMYCSYCLSEDQSWLLAVATDDRGEFLETVTINIDIPNRGRRKRASARRYGLQKLMDFILGLISQTVQPWRLVVGRIGRIGHGELKGWSWLLSKPNLQRASKHLKDICEQCSLMHPIAVPSILSACLVTLEPDSDLRVMSDQFTPDERFSQRSMQSPLSTPQDATCTHILVFPTSAKAQSAQASFSVIGELDLGEDLNMVIMDGDDDDDGINMMDVFKCWDDLPMQQINMPHSRPGSPSQFEGNQQSPGESGSKGAGSRDGYGSQDSEEVGLVLQQPLAIGYLVSTAPTGRMPEWFWSSCPHMENVCPVFLRTALHLHSPTILQNTDDPLQQNQSSTEHPLDSNITADVLRYVLEGYNLLSWLAMDSNTHDRLSCLPIHVQVLMQLYHMTAALA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VQ47</accession>
    <name>A0A6E8VQ47_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CUE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
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        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
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            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain>Mali-NIH</strain>
        </source>
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                <consortium name="EnsemblMetazoa"/>
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            <dbReference type="PROSITE" id="PS51140"/>
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        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="558" mass="59991" checksum="F09D18CA9648617F" modified="2020-08-12" version="1">MSPMVRSSPRMGLGRQGMVETTFTTNVTAAETDLTVAKINSMFPTVPETHIRMLLKKYYNREAVVISALQVEKHPLTTPGPYSTPPLGHRPFHNTAMGVVSAFQMTPVLGGRSEMSSSRTGSPVPRPASGASGSYAGGYGSPRNGPADGYRSSPKPHSSPKMKLRYMKSIFPKAEETLILDVLANADNNVQTASQQLLKMGYDKREQPVPQRSSASRKGTGTSQATEDDKKEELKTPTPKLKSPEEKKKIKARLQTKYKDTPEKIILMALESVDYSEERAKKILNIVIQEDRDKKKSVKIETPHKAEEDKENARASAGPSRSNSTASSQPRTPLLAKRAHDGSGVAVAPSQDPGKTTVDGTVKATEQQQEGAAAEASTTIAASLEADHSPGASTTESSSSDTTPDDSSSPDSSPATKLRDGDGVRSVGKQNRPKAKTDHAKTSSSEDESSEQQQMGTDGKLDGKSPQLASRIPTKGPNMKLFKGPNDDLLLTDYVTWNGANPDLAKGSQKKSTGPDRAIRTERTYKPHGPNAELCKGPVGSLAKGSMYTQLMKDVKCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VRR4</accession>
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        <submittedName>
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        <name type="scientific">Anopheles coluzzii</name>
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            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
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            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
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    <comment type="similarity">
        <text evidence="5">Belongs to the NRARP family.</text>
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        <property type="gene ID" value="ACON006665"/>
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    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
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        <source>
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    </evidence>
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    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00038386"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
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    </evidence>
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        <source>
            <dbReference type="EnsemblMetazoa" id="ACON006665-PB"/>
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    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VRU3</accession>
    <name>A0A6E8VRU3_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="13">C2H2-type domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VRU3"/>
    <dbReference type="EnsemblMetazoa" id="ACON006701-RC">
        <property type="protein sequence ID" value="ACON006701-PC"/>
        <property type="gene ID" value="ACON006701"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005746"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON006701"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040600"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000621">
        <property type="entry name" value="FLT3-interacting zinc finger 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001506">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000670">
        <property type="entry name" value="zinc finger protein 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000624">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF142">
        <property type="entry name" value="ZINC FINGER PROTEIN 384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13894">
        <property type="entry name" value="zf-C2H2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="20"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="48"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="76"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="104"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON006701-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="148" mass="16888" checksum="EFF4D913DF304AD7" modified="2020-08-12" version="1">MSQQAVESGGMKISNCSKPFACTECNKLFRQLSTLTNHMKIHTGEKPYKCTICMKEFRQTTTLSNHMKIHTGEKPFNCNFCDKQFRQLSTLSNHKKIHTGEKPFECSVCGKQFRQSSTLNSHIRIHSDDKFSSLYPPSHLDNIDLIGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VRW9</accession>
    <name>A0A6E8VRW9_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tubulin polymerization-promoting protein homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TPPP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VRW9"/>
    <dbReference type="EnsemblMetazoa" id="ACON006948-RA">
        <property type="protein sequence ID" value="ACON006948-PA"/>
        <property type="gene ID" value="ACON006948"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_001924"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON006948"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033388"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015631">
        <property type="term" value="F:tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001578">
        <property type="term" value="P:microtubule bundle formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046785">
        <property type="term" value="P:microtubule polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032273">
        <property type="term" value="P:positive regulation of protein polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000266">
        <property type="entry name" value="TPPP family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008907">
        <property type="entry name" value="TPP/p25"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12932">
        <property type="entry name" value="P25 ALPHA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12932:SF9">
        <property type="entry name" value="TUBULIN POLYMERIZATION-PROMOTING PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05517">
        <property type="entry name" value="p25-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="25"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="182"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="34"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="189"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00069104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON006948-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="226" mass="24352" checksum="FED08F2DCFC90307" modified="2020-08-12" version="1">MADEAQPPNGTDVVDKMTVEVAEMALDAKEAAGKEAAGGQPGAAEPAAQPSEKETNGSATPANKPVCSAAFKEQFKAFSKFGDTKSDGKHLTLSQSDKWMKQAKVIDKKITTTDTGIHFKKLKSMKLTYEDYNKFLDDLAKTKKVELDEIKNKMANCGAPGVHNATPGKAAETVARLTDTSKYTGSHKQRFDETGKGKGIAGRKDMVDQSGYVSGYGHKNTYDKTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VS50</accession>
    <name>A0A6E8VS50_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="10">MFS domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VS50"/>
    <dbReference type="EnsemblMetazoa" id="ACON007340-RB">
        <property type="protein sequence ID" value="ACON007340-PB"/>
        <property type="gene ID" value="ACON007340"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010207"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007340"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039401"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015149">
        <property type="term" value="F:hexose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17431">
        <property type="entry name" value="MFS_GLUT_Class1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000029">
        <property type="entry name" value="solute carrier family 2, facilitated glucose transporter member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045263">
        <property type="entry name" value="GLUT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23503:SF128">
        <property type="entry name" value="GLUCOSE TRANSPORTER TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23503">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 8" id="KW-0472">Membrane</keyword>
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    <keyword evidence="3 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
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        <location>
            <begin position="112"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="168"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="198"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="228"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="260"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="290"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="414"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="445"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="471"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="511"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="539"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="119"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="48"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007340-PB"/>
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    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="581" mass="64895" checksum="AD665D1DA013C553" modified="2020-08-12" version="1">MSYGDISYGLQKQVSVMSMNLSAKLDDLQRGDRHLETTVALCEIRTQLQELTKSVESCQTEVSEVKRDMVAIKHELDTVQQVKEEIEELREYVDRLEEHTHRRKLRLLEQGLTFFLTYAIFAAVLGMLQFGYNTGVINAPEVNIENFMKDVYKDRYGEDISEEFIQQLYSVAVSIFAIGGMLGGFSGGWMANRFGRKGGLLLNNVLGISGACLMGFTKMSHSYEMLFLGRFIIGVNCGLNTSLVPMYISEIAPLNLRGGLGTVNQLAVTVGLLLSQVLGIEQILGTNDGWPVLLGLAICPAILQLLLLPICPESPRYLLITKQWEEEARKALRRLRASNQVEEDIEEMRAEERAQQSESSISTIELICSPTLRAPLIIGIVMQLSQQFSGINAVFYYSTSLFMSSGLTEESAKFATIGIGAIMVVMTLVSIPLMDRTGRRTLHLYGLGGMFIFSIFITISFLIKAREMIDWMSYLSVVSTLAFVVFFAVGPGSIPWMITAELFSQGPRPSAMAIAVLVNWMANFVVGIGFPSLKTALENYTFLPFSVFLAIFWIFTYKKVPETKNKTFEEILALFRHGNGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VS62</accession>
    <name>A0A6E8VS62_ANOCL</name>
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        <submittedName>
            <fullName evidence="5">RRM domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">Ngousso</strain>
        </source>
    </reference>
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        <property type="gene ID" value="ACON007325"/>
    </dbReference>
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    <dbReference type="VEuPathDB" id="VectorBase:ACON007325"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035044"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12373">
        <property type="entry name" value="RRM_SRSF3_like"/>
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    </dbReference>
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        <property type="entry name" value="RNA-binding protein 1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
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            <begin position="11"/>
            <end position="84"/>
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            <begin position="78"/>
            <end position="124"/>
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            <begin position="93"/>
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        <location>
            <begin position="106"/>
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    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
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            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007325-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="124" mass="14651" checksum="E7662C6B8C217FB9" modified="2020-08-12" version="1">MARYREWDLQCKVYVGNLGSSASKHEIESAFGKYGPLRNVWVARNPPGFAFVEFEDKRDAEDAVRSLDGTRCCGTRIRVEMSSGRSRRDERTRRPRRSYRSRSRSMSRERDRDRRSRSGSRDRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VS69</accession>
    <name>A0A6E8VS69_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="3">SH3 domain-binding glutamic acid-rich protein homolog</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
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            <taxon>Insecta</taxon>
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            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SH3BGR family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VS69"/>
    <dbReference type="EnsemblMetazoa" id="ACON007362-RB">
        <property type="protein sequence ID" value="ACON007362-PB"/>
        <property type="gene ID" value="ACON007362"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002548"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007362"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040662"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006993">
        <property type="entry name" value="Glut_rich_SH3-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051033">
        <property type="entry name" value="SH3BGR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12232:SF15">
        <property type="entry name" value="SH3 DOMAIN-BINDING GLUTAMIC ACID-RICH PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12232">
        <property type="entry name" value="SH3 DOMAIN-BINDING GLUTAMIC ACID-RICH-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04908">
        <property type="entry name" value="SH3BGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="102"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="126"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007362-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="167" mass="18162" checksum="6D118C951DE7953E" modified="2020-08-12" version="1">MVIKVYISGMSGNKEVKKRQQRVTMIMDSKHIEYTVIDITEPGQEGEKDFMQTNAQHKGCTISDPNPRHALPPQLFNDTEYCGDYDDFDMANEVDNLEVFLKLAAPAAPEPEHKNGDETTAPPATGDDDNATTAAAEDEDENKENKTEDNNGAGAGDGAEAEGANEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VTV5</accession>
    <name>A0A6E8VTV5_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="10">MFS domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VTV5"/>
    <dbReference type="EnsemblMetazoa" id="ACON007753-RA">
        <property type="protein sequence ID" value="ACON007753-PA"/>
        <property type="gene ID" value="ACON007753"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006994"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007753"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_043216"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000218">
        <property type="entry name" value="facilitated trehalose transporter Tret1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050549">
        <property type="entry name" value="MFS_Trehalose_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48021">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48021:SF33">
        <property type="entry name" value="AT22075P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="58"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="87"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="111"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="146"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="176"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="261"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="298"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="325"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="359"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="395"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="422"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="1"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007753-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="462" mass="50292" checksum="DE89BBF7810E9F14" modified="2020-08-12" version="1">MFLEFLSRRYWKQFAAIAAVHIITASYGVTVGWPAPIIPLLRSPETPLPSGPVTVDEASWIGSTLCIGGTIGTILFAIIHTYFGKKIALLLTSVPHIILWTLILVGDNVWYIYGARFCSGLTGGGVVSVVPLYIADIADKKIRGTLGSLTIIFINIGLVFIYAAGNYLPYDLIPKIMLVSPVAFIVLVSFLPETPYCLLKKGRLLEAERSLMFYRNISDQQYQTAEFTTEFDELKSFVHTQSTQSRICWADFTTPEAKRGLFIGVFVMALNQFSGIFAILTYAGTILQMSGTGIDPNVALVLVAILNICGNLTSFAIIDRAGRKIMLLLSATGVGLALAVLGAHSYLLTIGYDLQGAEWLPVLALALTLFLGAIGITNIPFFIVPEVMPPKLRSIGSTISGTLLCMFAFVTVKLYPIMMVNIHIYGTVWISSGVCAIAVVIIIFLIPETKGKNLNVQSDSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VU76</accession>
    <name>A0A6E8VU76_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phosphorylated adapter RNA export protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">RNA U small nuclear RNA export adapter protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PHAX family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VU76"/>
    <dbReference type="EnsemblMetazoa" id="ACON008050-RB">
        <property type="protein sequence ID" value="ACON008050-PB"/>
        <property type="gene ID" value="ACON008050"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005362"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008050"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030133"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006408">
        <property type="term" value="P:snRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1440:FF:000001">
        <property type="entry name" value="phosphorylated adapter RNA export protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1440">
        <property type="entry name" value="PHAX RNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039047">
        <property type="entry name" value="PHAX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019385">
        <property type="entry name" value="PHAX_RNA-binding_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038092">
        <property type="entry name" value="PHAX_RNA-binding_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13135">
        <property type="entry name" value="CYTOSOLIC RESINIFERATOXIN BINDING PROTEIN RBP-26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13135:SF0">
        <property type="entry name" value="PHOSPHORYLATED ADAPTER RNA EXPORT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10258">
        <property type="entry name" value="PHAX_RNA-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Phosphorylated adapter RNA export protein RNA-binding" evidence="12">
        <location>
            <begin position="279"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="196"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="410"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="15"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="63"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="230"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="244"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF10258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008050-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="500" mass="55413" checksum="AA82818D1A21C06B" modified="2020-08-12" version="1">MMEHETLNVPSPKDDLEEGELSESDSDGYTPLARPEAAKSEFATASVNVPRQMDIVSDEHDDDELHHEKSDSDGSSDESGSNIRLGPMNYPKRMAGHSPRTAHKRPLHAHPPAVAAATAAASAAAAAASGANPKPMPHGTAARTKKYNIWTESLQEDTLMETMRGCGVTGSDMRNRDVESYDYKLRFRLQSGNAFERLKRRQSNSDDSDGYTGGGKRMRTGHNSYPEASGTERRGSIKDRIGKRNNSTDSNSDDCAQNAPRHIPDLEVGENCTNEKFGIELAEKLEETHTELMVRVAETLGQEIPMKLFKETQKIEADGGMLVMKGWRRRTPGGVFLFLLKHCEEVDEEAKRTIFQSEKKAKQKEWKLTKSINRDKKVEELKKTLNRQAANDAEQTSSSLPIMAHLKAETHSTLSNPPPSPVGEENFEGNSDFEARNIHVNVTTSPDKTLLLHGHDTDKDSDVAPEDACRMLPDGSTAMRNLASYQEDCLDITCDDMDLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VU80</accession>
    <name>A0A6E8VU80_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VU80"/>
    <dbReference type="EnsemblMetazoa" id="ACON008039-RA">
        <property type="protein sequence ID" value="ACON008039-PA"/>
        <property type="gene ID" value="ACON008039"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000098"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON008039"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032599"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004691">
        <property type="term" value="F:cAMP-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007476">
        <property type="term" value="P:imaginal disc-derived wing morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000005">
        <property type="entry name" value="cAMP-dependent protein kinase catalytic subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-DEPENDENT PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353:SF152">
        <property type="entry name" value="UT01108P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="1"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="7">
        <location>
            <begin position="243"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON008039-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="295" mass="34393" checksum="310CF0D92F15D74D" modified="2020-08-12" version="1">KLIKRKASGEYFAMKILSKERIVYQKQLQHTTNEKRILQSIRFPFVVNLEICYKDNSYLYLVMPFVNGGEMFTLLRHSRRFSESQAAFYGAQVALALEYLHGCNLIYRDLKPENLLVDYRGYVKMTDFGFCKLIKDRTWTLCGTPEYLAPEIIQAKGYGKSVDWWSYGVLLYEMAAGYSPFYVNSSDQMVLFERICKGKYKFPKSFSTDLCSLVHHLLQTDLSRRYGNLRNGCDDIKQHAWFKTINWYGLLNRELPAPYVPKLQGPGDASLFDVYDEQKLKIASKCLYAKEFADF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W009</accession>
    <name>A0A6E8W009_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Salivary secreted mucin 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8W009"/>
    <dbReference type="EnsemblMetazoa" id="ACON009473-RB">
        <property type="protein sequence ID" value="ACON009473-PB"/>
        <property type="gene ID" value="ACON009473"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_003604"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON009473"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032646"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="161"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="183"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="201"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009473-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="249" mass="27384" checksum="E4700D37ABF1FEAF" modified="2020-08-12" version="1">AASYEKDANSDASDDAEYIVVPLSHHRPTYYNRYPSSFEGFDGVVDTGDFAPVPARPVYFPSYNPFSWHLSGYLDDLLKRVRDRFSGSWNPFYGGDYLPSGPGFFPVEIPDDSSEDGVTNTTSTVKVIDGHKVVINDTYYTKKTEFGTSIFKVRVIDVKPTDDGSAEATTPKAPTDVEVGNRNGDDGAESDKEPAAPATDAPKRDTELESSDEDKTTVDDDNLNTIDTSNVDIDEAKERLAPEEAEEQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W1N2</accession>
    <name>A0A6E8W1N2_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Myosin heavy chain</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Muscle contraction.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Myofibril</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON010147-RB">
        <property type="protein sequence ID" value="ACON010147-PB"/>
        <property type="gene ID" value="ACON010147"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009622"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON010147"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041963"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031672">
        <property type="term" value="C:A band"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032982">
        <property type="term" value="C:myosin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008307">
        <property type="term" value="F:structural constituent of muscle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007298">
        <property type="term" value="P:border follicle cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040011">
        <property type="term" value="P:locomotion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006936">
        <property type="term" value="P:muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031033">
        <property type="term" value="P:myosin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007424">
        <property type="term" value="P:open tracheal system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045214">
        <property type="term" value="P:sarcomere organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14909">
        <property type="entry name" value="MYSc_Myh1_insects_crustaceans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.820:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000036">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000008">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.530:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.360:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.4820:FF:000002">
        <property type="entry name" value="Myosin heavy chain 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000038">
        <property type="entry name" value="Myosin heavy chain muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000019">
        <property type="entry name" value="Myosin heavy chain, isoform G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000021">
        <property type="entry name" value="Myosin heavy chain, isoform G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000025">
        <property type="entry name" value="Myosin heavy chain, isoform G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000009">
        <property type="entry name" value="Myosin heavy chain, isoform G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000010">
        <property type="entry name" value="Myosin heavy chain, isoform G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000005">
        <property type="entry name" value="Myosin heavy chain, isoform I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000024">
        <property type="entry name" value="Myosin heavy chain, isoform J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.720:FF:000001">
        <property type="entry name" value="Myosin heavy chain, muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000050">
        <property type="entry name" value="Myosin heavy chain, muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.370">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.360">
        <property type="entry name" value="Myosin S1 fragment, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008989">
        <property type="entry name" value="Myosin_S1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002928">
        <property type="entry name" value="Myosin_tail"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004009">
        <property type="entry name" value="SH3_Myosin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014751">
        <property type="entry name" value="XRCC4-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615:SF27">
        <property type="entry name" value="MYOSIN HEAVY CHAIN, MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615">
        <property type="entry name" value="MYOSIN HEAVY CHAIN, NON-MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02736">
        <property type="entry name" value="Myosin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01576">
        <property type="entry name" value="Myosin_tail_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90257">
        <property type="entry name" value="Myosin rod fragments"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51844">
        <property type="entry name" value="SH3_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 14" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="6 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 14" id="KW-0505">Motor protein</keyword>
    <keyword evidence="10" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="8 14" id="KW-0518">Myosin</keyword>
    <keyword evidence="5 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0787">Thick filament</keyword>
    <feature type="domain" description="Myosin N-terminal SH3-like" evidence="17">
        <location>
            <begin position="34"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="Myosin motor" evidence="16">
        <location>
            <begin position="87"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="14">
        <location>
            <begin position="657"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1823"/>
            <end position="1842"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1920"/>
            <end position="1961"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="180"/>
            <end position="187"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010147-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="20">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1961" mass="224202" checksum="19E9E1AE470ADF3E" modified="2020-08-12" version="1">MPKPPVQVGEDPDPTEFLFVSLEQKRIDQSKPYDSKKACWVPEEKEGYVLGEIKATKGELVTVALPGGEEKNFKKEQLSQVNPPKFEKVEDMADLTYLNEAAVLHNLRQRYYSKLIYTYSGLFCVVINPYKRYPLYTNRCAKMYRGKRRNEVPPHLFAVSDGAYVNMLTNHENQSMLITGESGAGKTENTKKVIAYFATIGASGKKDENAEKKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFTGSGKLAGADIETYLLEKARVISQQTLERSYHIFYQIMSGSVKGLKEKCLLSNNIHDYHIVAQGKTTIPSVDDGEEMQITDEAFNVLGFTQEEKDNIYRITSAVMHMGRMQFKQKGREEQAEADGTEDGDRVAKLLGVGTDDLYKNLLKPRIKVGNEFVTKGQNKDQVTNSVGALCKGIFDRLFKWLVKKCNETLDTKQKRAQFIGVLDIAGFEIFDNNGFNQLCINFTNERLQQFFNHYMFILEQEEYEREGIQWTFIDFGLDLQPTIDLIEKPMGILSILEEESMFPKATDQTFAEKLMTNHLGKSAPFMKPRPPKPGIPAGHFAIGHYAGVVSYNITGWLEKNKDPLNDTVVDQFKKGSNALMVEIFADHPGQSADPAAAKGGRGKKGAGFATVSSSYKEQLNNLMTTLKSTQPHFVRCIIPNEMKTAGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKLRYKILCPQLIKEPCSPEKVTQIVLTHIQLPEEQFRMGKTKVFFRAGVLGQMEEFRDERLSKIMSWMQAWCRGYLSRKEFKKMQEQRVSLEIVQRNLRKYLKLRTWAWWKLWQKVKPLLNVSRVEDQIAKLEEKATKAQEAYEKEEKLRKELEALNSKLLAEKTALLDSLSGEKGALQEYQEKAAKLTAQKNDLENQLRDTQERLAQEEDARNQLFQTKKKLEQEIGSQKKDAEDLELQIQKIEQDKASKDHQIRNLNDEIAHQDELINKLNKEKKMQGEVNQKTAEELQAAEDKVNHLNKVKAKLEQTLDELEDSLEREKKLRGDVEKAKRKVEGDLKLTQEAVADLERNKKELEQTVLRKDKEISALSAKLEDEQSLVGKLQKQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELAKLRRDLEEANIQHEGTLANLRKKHNDAVAEMAEQVDQLNKLKTKAEKERTQYFAELNDARIGCDQLSNEKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEDAESQVSQLSKIKISLTQQLEDTKRLADEEARERATLLGKFRNLEHDLDNLREQVEEEAEGKGDIQRQLSKANAEAQLWRSKYESEGVARAEELEEAKRKLQARLAEAEETIESLNQKCIALEKTKQRLATEVEDLQLEVDRASSIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKSRKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDVQSALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELSDAHEQLNEVSAQNASIAAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAESNALKGGKKAIQKLEQRVRELESELDSEQRRHADAQKNLRKSERRIKELTFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADIAEQAATKFRTKGGRAGSVQRGASPAPQRQPSAMPALAGLNLPTFDDHGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W1N7</accession>
    <name>A0A6E8W1N7_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Myosin heavy chain</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Muscle contraction.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Myofibril</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040234093.1">
        <property type="nucleotide sequence ID" value="XM_040378159.2"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON010147-RG">
        <property type="protein sequence ID" value="ACON010147-PG"/>
        <property type="gene ID" value="ACON010147"/>
    </dbReference>
    <dbReference type="GeneID" id="120956570"/>
    <dbReference type="CTD" id="35007"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009622"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON010147"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041963"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031672">
        <property type="term" value="C:A band"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032982">
        <property type="term" value="C:myosin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008307">
        <property type="term" value="F:structural constituent of muscle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007298">
        <property type="term" value="P:border follicle cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040011">
        <property type="term" value="P:locomotion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006936">
        <property type="term" value="P:muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031033">
        <property type="term" value="P:myosin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007424">
        <property type="term" value="P:open tracheal system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045214">
        <property type="term" value="P:sarcomere organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14909">
        <property type="entry name" value="MYSc_Myh1_insects_crustaceans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.820:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000036">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000008">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.530:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.360:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.4820:FF:000002">
        <property type="entry name" value="Myosin heavy chain 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000038">
        <property type="entry name" value="Myosin heavy chain muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000019">
        <property type="entry name" value="Myosin heavy chain, isoform G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000021">
        <property type="entry name" value="Myosin heavy chain, isoform G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000025">
        <property type="entry name" value="Myosin heavy chain, isoform G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000009">
        <property type="entry name" value="Myosin heavy chain, isoform G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000010">
        <property type="entry name" value="Myosin heavy chain, isoform G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000005">
        <property type="entry name" value="Myosin heavy chain, isoform I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000024">
        <property type="entry name" value="Myosin heavy chain, isoform J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.720:FF:000001">
        <property type="entry name" value="Myosin heavy chain, muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000050">
        <property type="entry name" value="Myosin heavy chain, muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.370">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.360">
        <property type="entry name" value="Myosin S1 fragment, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008989">
        <property type="entry name" value="Myosin_S1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002928">
        <property type="entry name" value="Myosin_tail"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004009">
        <property type="entry name" value="SH3_Myosin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014751">
        <property type="entry name" value="XRCC4-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615:SF27">
        <property type="entry name" value="MYOSIN HEAVY CHAIN, MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615">
        <property type="entry name" value="MYOSIN HEAVY CHAIN, NON-MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02736">
        <property type="entry name" value="Myosin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01576">
        <property type="entry name" value="Myosin_tail_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90257">
        <property type="entry name" value="Myosin rod fragments"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51844">
        <property type="entry name" value="SH3_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 14" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="6 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 14" id="KW-0505">Motor protein</keyword>
    <keyword evidence="10" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="8 14" id="KW-0518">Myosin</keyword>
    <keyword evidence="5 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0787">Thick filament</keyword>
    <feature type="domain" description="Myosin N-terminal SH3-like" evidence="17">
        <location>
            <begin position="34"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="Myosin motor" evidence="16">
        <location>
            <begin position="87"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="14">
        <location>
            <begin position="657"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1823"/>
            <end position="1842"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1920"/>
            <end position="1961"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="180"/>
            <end position="187"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010147-PG"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="20">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1961" mass="224247" checksum="C2C3180F938E4FFA" modified="2020-08-12" version="1">MPKPPVQVGEDPDPTEFLFVSLEQKRIDQSKPYDSKKACWVPEEKEGYVLGEIKATKGELVTVALPGGEEKNFKKEQLSQVNPPKFEKVEDMADLTYLNEAAVLHNLRQRYYSKLIYTYSGLFCVVINPYKRYPLYTNRCAKMYRGKRRNEVPPHLFAVSDGAYVNMLTNHENQSMLITGESGAGKTENTKKVIAYFATIGASGKKDENAEKKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFTGSGKLAGADIETYLLEKARVISQQTLERSYHIFYQIMSGSVKGLKEKCLLSNNIHDYHIVAQGKTTIPSVDDGEEMQITDEAFNVLGFTQEEKDNIYRITSAVMHMGRMQFKQKGREEQAEADGTEDGDRVAKLLGVGTDDLYKNLLKPRIKVGNEFVTKGQNKDQVTNSVGALCKGIFDRLFKWLVKKCNETLDTKQKRAQFIGVLDIAGFEIFDFNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYQREGIEWTFIDFGMDLQQCIELIEKPMGILSILEEESMFPKATDQTFAEKLMTNHLGKSAPFMKPRPPKPGIPAGHFAIGHYAGVVSYNITGWLEKNKDPLNDTVVDQFKKGSNALMVEIFADHPGQSADPAAAKGGRGKKGAGFATVSSSYKEQLNNLMTTLKSTQPHFVRCIIPNEMKTAGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKLRYKILCPQLIKEPCSPEKVTQIVLTHIQLPEEQFRMGKTKVFFRAGVLGQMEEFRDERLSKIMSWMQAWCRGYLSRKEFKKMQEQRVSLEIVQRNLRKYLKLRTWAWWKLWQKVKPLLNVSRVEDQIAKLEEKATKAQEAYEKEEKLRKELEALNSKLLAEKTALLDSLSGEKGALQEYQEKAAKLTAQKNDLENQLRDTQERLAQEEDARNQLFQTKKKLEQEIGSQKKDAEDLELQIQKIEQDKASKDHQIRNLNDEIAHQDELINKLNKEKKMQGEVNQKTAEELQAAEDKVNHLNKVKAKLEQTLDELEDSLEREKKLRGDVEKAKRKVEGDLKLTQEAVADLERNKKELEQTVLRKDKEISALSAKLEDEQSLVGKLQKQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELAKLRRDLEEANIQHEGTLANLRKKHNDAVAEMAEQVDQLNKLKTKAEKERTQYFAELNDARIGCDQLSNEKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEDAESQVSQLSKIKISLTQQLEDTKRLADEEARERATLLGKFRNLEHDLDNLREQVEEEAEGKGDIQRQLSKANAEAQLWRSKYESEGVARAEELEEAKRKLQARLAEAEETIESLNQKCIALEKTKQRLATEVEDLQLEVDRASSIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKSRKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDVQSALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELSDAHEQLNEVSAQNASIAAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAESNALKGGKKAIQKLEQRVRELESELDSEQRRHADAQKNLRKSERRIKELTFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADIAEQAATKFRTKGGRAGSVQRGASPAPQRQPSAMPALAGLNLPTFDDHGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W1P8</accession>
    <name>A0A6E8W1P8_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DH domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8W1P8"/>
    <dbReference type="EnsemblMetazoa" id="ACON010473-RD">
        <property type="protein sequence ID" value="ACON010473-PD"/>
        <property type="gene ID" value="ACON010473"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004053"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON010473"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037586"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030139">
        <property type="term" value="C:endocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043542">
        <property type="term" value="P:endothelial cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007266">
        <property type="term" value="P:Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040181">
        <property type="entry name" value="PKHG5/7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13217:SF11">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY DOMAIN-CONTAINING FAMILY G MEMBER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13217">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY DOMAIN-CONTAINING FAMILY G MEMBER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DH" evidence="2">
        <location>
            <begin position="560"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="445"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="901"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="450"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="475"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="904"/>
            <end position="921"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010473-PD"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="973" mass="108470" checksum="BC9A2D002EB789B3" modified="2020-08-12" version="1">MPSKANESTRSESNADNSVGGLASVSEQPVGPKMERSNSFFLTRKLSSLYSKLSGSRENVSTTNAVSPVTEALPFQFVRSRSMSSIQLKRAYRRSQNESTLQNLHEDAILERSESTEETAAVAGPEVATAVPEVTPPPIPRFERSNSILASIRRKISFRSDRRSSNVSNWSASLQNLRQEDFMISYDDLSFIDYDQFNSYETNILKRRSSRVLEPGTKGSFMEDISSLTNCAKSVPTSEHLETVPVTEATNGGVVRRRRKNDTLLRTTLIRKSMDVEGNLDRDKNVYRNSLDADKLMCISNVNRKSFRWSADMKSLSLTHLDEIERQATTPTIITNDPHVRKVSSSEYFSVCFESDQNDPGVLIPATKGVRLAECLRASLSRRSLSFSQIYVTDNGFNNPHETGLRYTEPLDANMDISALAGRTLIVSERDGAPNRRTGGHLTLQKAASVGNQPSIMSSNSKLFTSTSTDDNYDQSPKASSASGKQSKQKWTIPFGSKGPQKSKLCELLDSYNKEIPKSTSSSSNFNNPEYVDALVGLRNLPQCWTDIVNCAGMSEAETKIQSAIWELVTTEVYYILALQTVTDLFLACLEDIQSQNILTDVDQNKLFSNIRDIWEANLRFWTLYLHPMVKHSQRTKDPMSIYYFQRGFVDFATIFTPYTKYCAEQSTCQFYCKEMYHNNALFMTYCAWCESQKMCNRLRLADILVRPMQRLTKYGLLLAAIKKHINDEAEGEAIDAMIHSVEEFVAGVNSHLTTRQESERLKGINARIDCYEVVDSNNETLDRLVKQHSIMFDLCQPMRGIDHGRKVFVEGDLKYKDGTGKNTLSVPGTSNSQSHSGMVLLSPSQRGISYPPPSPTRATLRRGFAFSTSIKNPPLIKTRNVSSQQSFALSQQQSFNSASLAQHQQCLPSPSNTLHGAPNNSGTNSAIEGCAVASGLTLSSANVSFFKRKNSFQSVPNNSATSSVDQSLPNET</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W1Q7</accession>
    <name>A0A6E8W1Q7_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">carnitine O-palmitoyltransferase</fullName>
            <ecNumber evidence="5">2.3.1.21</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>(R)-carnitine + hexadecanoyl-CoA = O-hexadecanoyl-(R)-carnitine + CoA</text>
            <dbReference type="Rhea" id="RHEA:12661"/>
            <dbReference type="ChEBI" id="CHEBI:16347"/>
            <dbReference type="ChEBI" id="CHEBI:17490"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="EC" id="2.3.1.21"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:12662"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism; fatty acid beta-oxidation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the carnitine/choline acetyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.21" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8W1Q7"/>
    <dbReference type="EnsemblMetazoa" id="ACON010164-RA">
        <property type="protein sequence ID" value="ACON010164-PA"/>
        <property type="gene ID" value="ACON010164"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON010164-RC">
        <property type="protein sequence ID" value="ACON010164-PC"/>
        <property type="gene ID" value="ACON010164"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011637"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON010164"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038071"/>
    <dbReference type="UniPathway" id="UPA00659"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004095">
        <property type="term" value="F:carnitine O-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009437">
        <property type="term" value="P:carnitine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.70:FF:000001">
        <property type="entry name" value="Carnitine O-palmitoyltransferase 1, liver isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.10:FF:000042">
        <property type="entry name" value="Carnitine Palmitoyl Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.70">
        <property type="entry name" value="Choline/Carnitine o-acyltransferase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000542">
        <property type="entry name" value="Carn_acyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039551">
        <property type="entry name" value="Cho/carn_acyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042231">
        <property type="entry name" value="Cho/carn_acyl_trans_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032476">
        <property type="entry name" value="CPT_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22589">
        <property type="entry name" value="CARNITINE O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22589:SF31">
        <property type="entry name" value="CARNITINE O-PALMITOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00755">
        <property type="entry name" value="Carn_acyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16484">
        <property type="entry name" value="CPT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00439">
        <property type="entry name" value="ACYLTRANSF_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Carnitine O-palmitoyltransferase N-terminal" evidence="18">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="Choline/carnitine acyltransferase" evidence="17">
        <location>
            <begin position="174"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="16">
        <location>
            <position position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR600542-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF16484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010164-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="787" mass="90192" checksum="F53D8289F0076E91" modified="2020-08-12" version="1">MAEAHSAVAFSFSITHEGWDINYDREVLDLVWQSGVRSWKKRLARFRTGVRNGVYPAHLQSLWLVIAIAVGLHFTQRHVPFDLVNKWLSVLPGDSLQWQLTACSLAGLIVWLSICYTMRYSLKLLLMYKGWMYEKRAPGSRVSLQTRLWGLFVKVLATWNKPGLYSFQGSLPRLPLPSVQDTMQRYLRSVRPLLDDSNYNRMERLAKEFEQGISTKLQRYLFLKSWWSTNYVSDWWEEYVYLRGRGALMVNSNFYGIDAIFMHPTKVQSARAASVVNLLLQFRRTVERQELEPILVQGLVPLCSWQYERIFNTVRAPGVETDKIIHYQDSNHIVVLYRGCYYKVIIYHNGRLLRPCELQIQIEHILQQSEEKPQPGEELLASLTAGDRTKWAQIRQTVFAKGVNRTSLHTVESAAFVLSLDEKPFEFDLAHPEKLDQFGRYLLHGNGHDRWFDKSFTVCVGSNGRIGFNAEHTWADAAVMSHIWEMLIMGETEQRYDENGNTVGIPEFEPPTPKRLTWDLNDKVALVAIDEAHIAAQKLLNDVDLRILVHDAYGKGLMKTCRLSPDAFIQMALQLAYYRDAGKFSLTYEASMTRLFREGRTETVRPCTIESAAWVKAMLDSSVSTEDRVRLLNEACERHQLGYQDAMCGKGIDRHLFCLYVVSKYLEIDSPFLQEVLSEPWRLSTSQTPHGQTAKMDLKKHPNCISAGGGFGPVADDGYGVSYIVAGEDLIFFHISSKRSCGTTSSERFAQEICRALADMKHLFEEYRSLQKKNPSNGTKSDKPQAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W214</accession>
    <name>A0A6E8W214_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Protein detached</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">Ngousso</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="12">Component of the dystrophin associated protein complex (DAPC). Interacts with Dg, via the Dg WW domain binding sites.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <location evidence="2">Sarcolemma</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8W214"/>
    <dbReference type="EnsemblMetazoa" id="ACON010261-RA">
        <property type="protein sequence ID" value="ACON010261-PA"/>
        <property type="gene ID" value="ACON010261"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011715"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON010261"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_029987"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016010">
        <property type="term" value="C:dystrophin-associated glycoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031594">
        <property type="term" value="C:neuromuscular junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042383">
        <property type="term" value="C:sarcolemma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050699">
        <property type="term" value="F:WW domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030010">
        <property type="term" value="P:establishment of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008586">
        <property type="term" value="P:imaginal disc-derived wing vein morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007474">
        <property type="term" value="P:imaginal disc-derived wing vein specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048790">
        <property type="term" value="P:maintenance of presynaptic active zone structure"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046716">
        <property type="term" value="P:muscle cell cellular homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007274">
        <property type="term" value="P:neuromuscular synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046928">
        <property type="term" value="P:regulation of neurotransmitter secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048172">
        <property type="term" value="P:regulation of short-term neuronal synaptic plasticity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd16242">
        <property type="entry name" value="EFh_DMD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00176">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02334">
        <property type="entry name" value="ZZ_dystrophin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.90:FF:000001">
        <property type="entry name" value="Dystrophin isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015153">
        <property type="entry name" value="EF-hand_dom_typ1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015154">
        <property type="entry name" value="EF-hand_dom_typ2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050774">
        <property type="entry name" value="KCMF1/Dystrophin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018159">
        <property type="entry name" value="Spectrin/alpha-actinin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000433">
        <property type="entry name" value="Znf_ZZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043145">
        <property type="entry name" value="Znf_ZZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12268:SF14">
        <property type="entry name" value="DYSTROPHIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12268">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE KCMF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09068">
        <property type="entry name" value="EF-hand_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09069">
        <property type="entry name" value="EF-hand_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00569">
        <property type="entry name" value="ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00150">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00291">
        <property type="entry name" value="ZnF_ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46966">
        <property type="entry name" value="Spectrin repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01357">
        <property type="entry name" value="ZF_ZZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50135">
        <property type="entry name" value="ZF_ZZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="WW" evidence="17">
        <location>
            <begin position="344"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="ZZ-type" evidence="18">
        <location>
            <begin position="601"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="815"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="914"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="780"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="862"/>
            <end position="892"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00065906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00083840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010261-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1064" mass="119958" checksum="489FD5E521AE5850" modified="2020-08-12" version="1">MDLQSEIDANRKVYEGLDNTGRKLLRSLTSQEDAVMLQHKLDEMNQRWNHLNSRSAAIRNRLESNSDHWNALLLSSRELTEWVIRKNTELTSIGFGSINGDSNSLQMQLDEHKAFRRQLDDKRSIIENNLMNGRQYISNESPLSDASDSEAIDETMYISTEEQNRILSRSIRREVNKLSEQWSLLIERCDKWKHRLDENITKMRQFQKILEDLSSRVASAETITHSWTTPAPGSDTTEEMQHLQRLKDKLTTANALLDDCNEQQNFFSSCRVIVPSPYLAKLEDINTRMKLLHIAVEERFKVLQQSMQTTAEDGIVGSHHEEGGKFSKPLHIAAGSSTVPNLEKSVQIPWERAVTPANVPYYINHERESTHWDHPEMIELMKSLADLNEVRFSAYRTALKLRTVQKKLAFDRLNMSVAIEIFDRYGLRAQNDKLIDIPDMTTILNSLYTTLEPIDRAVMLDLAINWVLNVYDSQRTGQIRVLSFKVGLILLCRGHLEEKYRYLFRLIADLEKKVDQRKMGLLLHDCIQVPRQLGEVAAFGGSNIEPSVRSCFELAGGVNQNGELTETAIEAQHFLSWLQHEPQSIVWLPVLHRLVAAETAKHQAKCNICKEYPIEGFRYRCLKCFNFDMCQKCFFTGRSTKNHKLSHPMHEYCTTATSTEDVRDFTRALRNKFKSRKYFKKHPRVGYLPVQSVLEGDALESPIPSPQHGTHTLQNDMHSRLEMYASRLAQVECGTRSNSTPDSDDEHQLIAQYCQALPAANAVGNSTGSGGPKSPVQVMAAMEAEQREELETMIKELEEENANLQAEYEKLKAKKTSTPITTPDEGYKTPTSTSNAIVSSVTTSSSGGSDMVTEAKLLRQHKGRLEARMQILEDHNKQLEAQLQRLRQLLHEPTIPKPQTLQTRSVTASQLNTDSPAKMQHNGHYDHKMSSESSQKPASFYGAVGGGSVNGFLSMAGEEMRQREQYSVDAFDKTGAGVAAVTNAFAANKPAGSNGGGVGIGSSGANSNIMSSEYTMDERPPPPPHSSLLHMADDLNKAVEELVHIMTTESKDDLQNDSTNIGLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W223</accession>
    <name>A0A6E8W223_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Myosin heavy chain</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Muscle contraction.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Myofibril</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON010147-RA">
        <property type="protein sequence ID" value="ACON010147-PA"/>
        <property type="gene ID" value="ACON010147"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009622"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON010147"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041963"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031672">
        <property type="term" value="C:A band"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032982">
        <property type="term" value="C:myosin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008307">
        <property type="term" value="F:structural constituent of muscle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007298">
        <property type="term" value="P:border follicle cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040011">
        <property type="term" value="P:locomotion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006936">
        <property type="term" value="P:muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031033">
        <property type="term" value="P:myosin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007424">
        <property type="term" value="P:open tracheal system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045214">
        <property type="term" value="P:sarcomere organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14909">
        <property type="entry name" value="MYSc_Myh1_insects_crustaceans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.820:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000036">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000008">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.530:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.360:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.4820:FF:000002">
        <property type="entry name" value="Myosin heavy chain 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000038">
        <property type="entry name" value="Myosin heavy chain muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000019">
        <property type="entry name" value="Myosin heavy chain, isoform G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000021">
        <property type="entry name" value="Myosin heavy chain, isoform G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000025">
        <property type="entry name" value="Myosin heavy chain, isoform G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000009">
        <property type="entry name" value="Myosin heavy chain, isoform G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000010">
        <property type="entry name" value="Myosin heavy chain, isoform G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000005">
        <property type="entry name" value="Myosin heavy chain, isoform I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000024">
        <property type="entry name" value="Myosin heavy chain, isoform J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.720:FF:000001">
        <property type="entry name" value="Myosin heavy chain, muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000050">
        <property type="entry name" value="Myosin heavy chain, muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.370">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.360">
        <property type="entry name" value="Myosin S1 fragment, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008989">
        <property type="entry name" value="Myosin_S1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002928">
        <property type="entry name" value="Myosin_tail"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004009">
        <property type="entry name" value="SH3_Myosin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014751">
        <property type="entry name" value="XRCC4-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615:SF27">
        <property type="entry name" value="MYOSIN HEAVY CHAIN, MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615">
        <property type="entry name" value="MYOSIN HEAVY CHAIN, NON-MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02736">
        <property type="entry name" value="Myosin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01576">
        <property type="entry name" value="Myosin_tail_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90257">
        <property type="entry name" value="Myosin rod fragments"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51844">
        <property type="entry name" value="SH3_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 14" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="6 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 14" id="KW-0505">Motor protein</keyword>
    <keyword evidence="10" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="8 14" id="KW-0518">Myosin</keyword>
    <keyword evidence="5 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0787">Thick filament</keyword>
    <feature type="domain" description="Myosin N-terminal SH3-like" evidence="17">
        <location>
            <begin position="34"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="Myosin motor" evidence="16">
        <location>
            <begin position="87"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="14">
        <location>
            <begin position="657"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1823"/>
            <end position="1864"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1919"/>
            <end position="1943"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="180"/>
            <end position="187"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010147-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="20">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1943" mass="222135" checksum="FA81F96061AC9D25" modified="2020-08-12" version="1">MPKPPVQVGEDPDPTEFLFVSLEQKRIDQSKPYDSKKACWVPEEKEGYVLGEIKATKGELVTVALPGGEEKNFKKEQLSQVNPPKFEKVEDMADLTYLNEAAVLHNLRQRYYSKLIYTYSGLFCVVINPYKRYPLYTNRCAKMYRGKRRNEVPPHLFAVSDGAYVNMLTNHENQSMLITGESGAGKTENTKKVIAYFATIGASGKKDENAEKKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFTGSGKLAGADIETYLLEKARVISQQTLERSYHIFYQIMSGSVKGLKEKCLLSNNIHDYHIVAQGKTTIPSVDDGEEMQITDEAFNVLGFTQEEKDNIYRITSAVMHMGRMQFKQKGREEQAEADGTEDGDRVAKLLGVGTDDLYKNLLKPRIKVGNEFVTKGQNKDQVTNSVGALCKGIFDRLFKWLVKKCNETLDTKQKRAQFIGVLDIAGFEIFDFNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGINWAFIDFGMDLLACVELIEKPMGILSILEEESMFPKATDQTFAEKLMTNHLGKSAPFMKPRPPKPGIPAGHFAIGHYAGVVSYNITGWLEKNKDPLNDTVVDQFKKGSNALMVEIFADHPGQSADPAAAKGGRGKKGAGFATVSSSYKEQLNNLMTTLKSTQPHFVRCIIPNEMKTAGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKLRYKILCPQLIKEPCSPEKVTQIVLTHIQLPEEQFRMGKTKVFFRAGVLGQMEEFRDERLSKIMSWMQAWCRGYLSRKEFKKMQEQRVSLEIVQRNLRKYLKLRTWAWWKLWQKVKPLLNVSRVEDQIAKLEEKATKAQEAYEKEEKLRKELEALNSKLLAEKTALLDSLSGEKGALQEYQEKAAKLTAQKNDLENQLRDTQERLAQEEDARNQLFQTKKKLEQEIGSQKKDAEDLELQIQKIEQDKASKDHQIRNLNDEIAHQDELINKLNKEKKMQGEVNQKTAEELQAAEDKVNHLNKVKAKLEQTLDELEDSLEREKKLRGDVEKAKRKVEGDLKLTQEAVADLERNKKELEQTVLRKDKEISALSAKLEDEQSLVGKLQKQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELAKLRRDLEEANIQHEGTLANLRKKHNDAVAEMAEQVDQLNKLKTKAEKERTQYFAELNDARIGCDQLSNEKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEDAESQVSQLSKIKISLTQQLEDTKRLADEEARERATLLGKFRNLEHDLDNLREQVEEEAEGKGDIQRQLSKANAEAQLWRSKYESEGVARAEELEEAKRKLQARLAEAEETIESLNQKCIALEKTKQRLATEVEDLQLEVDRASSIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKSRKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDVQSALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELSDAHEQLNEVSAQNASIAAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAESNALKGGKKAIQKLEQRVRELESELDSEQRRHADAQKNLRKSERRIKELTFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADIAEQAATKFRTKGGRAGSVQRGASPAPSVVRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W229</accession>
    <name>A0A6E8W229_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Myosin heavy chain</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Muscle contraction.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Myofibril</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON010147-RF">
        <property type="protein sequence ID" value="ACON010147-PF"/>
        <property type="gene ID" value="ACON010147"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009622"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON010147"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041963"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031672">
        <property type="term" value="C:A band"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032982">
        <property type="term" value="C:myosin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008307">
        <property type="term" value="F:structural constituent of muscle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007298">
        <property type="term" value="P:border follicle cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040011">
        <property type="term" value="P:locomotion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006936">
        <property type="term" value="P:muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031033">
        <property type="term" value="P:myosin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007424">
        <property type="term" value="P:open tracheal system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045214">
        <property type="term" value="P:sarcomere organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14909">
        <property type="entry name" value="MYSc_Myh1_insects_crustaceans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.820:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000036">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000008">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.530:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.360:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.4820:FF:000002">
        <property type="entry name" value="Myosin heavy chain 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000038">
        <property type="entry name" value="Myosin heavy chain muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000019">
        <property type="entry name" value="Myosin heavy chain, isoform G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000021">
        <property type="entry name" value="Myosin heavy chain, isoform G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000025">
        <property type="entry name" value="Myosin heavy chain, isoform G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000009">
        <property type="entry name" value="Myosin heavy chain, isoform G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000010">
        <property type="entry name" value="Myosin heavy chain, isoform G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000005">
        <property type="entry name" value="Myosin heavy chain, isoform I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000024">
        <property type="entry name" value="Myosin heavy chain, isoform J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.720:FF:000001">
        <property type="entry name" value="Myosin heavy chain, muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000050">
        <property type="entry name" value="Myosin heavy chain, muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.370">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.360">
        <property type="entry name" value="Myosin S1 fragment, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008989">
        <property type="entry name" value="Myosin_S1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002928">
        <property type="entry name" value="Myosin_tail"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004009">
        <property type="entry name" value="SH3_Myosin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014751">
        <property type="entry name" value="XRCC4-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140">
        <property type="entry name" value="MYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140:SF857">
        <property type="entry name" value="MYOSIN-11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02736">
        <property type="entry name" value="Myosin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01576">
        <property type="entry name" value="Myosin_tail_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90257">
        <property type="entry name" value="Myosin rod fragments"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51844">
        <property type="entry name" value="SH3_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 14" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="6 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 14" id="KW-0505">Motor protein</keyword>
    <keyword evidence="10" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="8 14" id="KW-0518">Myosin</keyword>
    <keyword evidence="5 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0787">Thick filament</keyword>
    <feature type="domain" description="Myosin N-terminal SH3-like" evidence="17">
        <location>
            <begin position="34"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="Myosin motor" evidence="16">
        <location>
            <begin position="87"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="14">
        <location>
            <begin position="657"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1823"/>
            <end position="1842"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1920"/>
            <end position="1961"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="180"/>
            <end position="187"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010147-PF"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="20">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1961" mass="224099" checksum="C104D978DAB28F1C" modified="2020-08-12" version="1">MPKPPVQVGEDPDPTEFLFVSLEQKRIDQSKPYDSKKACWVPEEKEGYVLGEIKATKGELVTVALPGGEEKNFKKEQLSQVNPPKFEKVEDMADLTYLNEAAVLHNLRQRYYSKLIYTYSGLFCVVINPYKRYPLYTNRCAKMYRGKRRNEVPPHLFAVSDGAYVNMLTNHENQSMLITGESGAGKTENTKKVIAYFATIGASGKKDENAEKKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFTGSGKLAGADIETYLLEKARVISQQTLERSYHIFYQIMSGSVKGLKEKCFLSNDVYDYMIIAQGKTTIPNVDDGEEMGLTDEAFNVLGFTQEEKDNIYRITSAVMHMGRMQFKQKGREEQAEADGTEDGDRVAKLLGVGTDDLYKNLLKPRIKVGNEFVTKGQNKDQVTNSVGALCKGIFDRLFKWLVKKCNETLDTKQKRAQFIGVLDIAGFEIFDFNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGINWAFIDFGMDLLACVELIEKPMGILSILEEESMFPKATDQTFAEKLMTNHLGKSAPFMKPRPPKPGIPAGHFAIGHYAGVVSYNITGWLEKNKDPLNDTVVDQFKKGSNALMVEIFADHPGQSADPAAAKGGRGKKGAGFATVSSSYKEQLNNLMTTLKSTQPHFVRCIIPNEMKTAGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKLRYKILCPQLIKEPCSPEKVTQIVLTHIQLPEEQFRMGKTKVFFRAGVLGQMEEFRDERLSKIMSWMQAWCRGYLSRKEFKKMQEQRVSLEIVQRNLRKYLKLRTWAWWKLWQKVKPLLNVSRVEDQIAKLEEKATKAQEAYEKEEKLRKELEALNSKLLAEKTALLDSLSGEKGALQEYQEKAAKLTAQKNDLENQLRDTQERLAQEEDARNQLFQTKKKLEQEIGSQKKDAEDLELQIQKIEQDKASKDHQIRNLNDEIAHQDELINKLNKEKKMQGEVNQKTAEELQAAEDKVNHLNKVKAKLEQTLDELEDSLEREKKLRGDVEKAKRKVEGDLKLTQEAVADLERNKKELEQTVLRKDKEISALSAKLEDEQSLVGKLQKQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELAKLRRDLEEANIQHEGTLANLRKKHNDAVAEMAEQVDQLNKLKTKAEKERTQYFAELNDARIGCDQLSNEKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEDAESQVSQLSKIKISLTQQLEDTKRLADEEARERATLLGKFRNLEHDLDNLREQVEEEAEGKGDIQRQLSKANAEAQLWRSKYESEGVARAEELEEAKRKLQARLAEAEETIESLNQKCIALEKTKQRLATEVEDLQLEVDRASSIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKSRKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDVQSALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELSDAHEQLNEVSAQNASIAAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAESNALKGGKKAIQKLEQRVRELESELDSEQRRHADAQKNLRKSERRIKELTFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADIAEQAATKFRTKGGRAGSVQRGASPAPQRQPSAMPALAGLNLPTFDDHGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W3U7</accession>
    <name>A0A6E8W3U7_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Receptor expression-enhancing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8W3U7"/>
    <dbReference type="EnsemblMetazoa" id="ACON011331-RC">
        <property type="protein sequence ID" value="ACON011331-PC"/>
        <property type="gene ID" value="ACON011331"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000364"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON011331"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042433"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005881">
        <property type="term" value="C:cytoplasmic microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071782">
        <property type="term" value="C:endoplasmic reticulum tubular network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071786">
        <property type="term" value="P:endoplasmic reticulum tubular network organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004345">
        <property type="entry name" value="TB2_DP1_HVA22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12300">
        <property type="entry name" value="HVA22-LIKE PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12300:SF117">
        <property type="entry name" value="LP05237P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03134">
        <property type="entry name" value="TB2_DP1_HVA22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU362006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON011331-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="185" mass="21402" checksum="052F053A011BB927" modified="2020-08-12" version="1">MLSRIPFPRMSVTGMGLCSLVAIFFCPRAIGSNILFPGFRLLFGTLYPAYASYKAVRTKNVKEYVKWMMYWIVFAFFTFIETFTDILLSWFPFYYEIKVIVVLWLLSPATRGSSTLYRKFVHPMLTRREQEIDDYINQAKEKGYTAVLQLGSKGVNYATNVIMQTAIKHVPTEQLLNLTLKSNES</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W3U8</accession>
    <name>A0A6E8W3U8_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="3">PX domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8W3U8"/>
    <dbReference type="EnsemblMetazoa" id="ACON012012-RA">
        <property type="protein sequence ID" value="ACON012012-PA"/>
        <property type="gene ID" value="ACON012012"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012060"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON012012"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042602"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034498">
        <property type="term" value="P:early endosome to Golgi transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06866">
        <property type="entry name" value="PX_SNX8_Mvp1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028662">
        <property type="entry name" value="SNX8/Mvp1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035704">
        <property type="entry name" value="SNX8/Mvp1_PX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46571">
        <property type="entry name" value="SORTING NEXIN-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46571:SF1">
        <property type="entry name" value="SORTING NEXIN-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PX" evidence="2">
        <location>
            <begin position="16"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON012012-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="372" mass="42724" checksum="CCB0ACFB2B1A0386" modified="2020-08-12" version="1">MSIASVGDDSTERELNESPVSVQIVPEKKGLFLKHSEYEIKLKGIEKTVRRRYKDFVTLHRYLAEKYPYRLLPALPPKQLMLDSLLEERRRGLQTWLIIVSLHPVLGSSPIVSTFLRDTTTDHQYRLRVTYEKQMDELVRLRPDATLPEVDLDTLAESRTRLHRVQCSMGRLKQLFDRRLGHNRRQTGECAEIERILQNPDLRDAFNEDATFEDLSASERLIAKQVERYVQVQQRAVTERLDVLLEVLHAHSQLCERIEKSIVADQQRTLTGKSPGANAGSSRVKVKAHSMNQTGTMAQQNEGNGSAFEATQLARRSAFAFSCAQAETLLAERYLQSLPSILLSYAHEEQQHHQKMSKIWHRLVVSESSRLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W3W0</accession>
    <name>A0A6E8W3W0_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Sulfatase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sulfatase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8W3W0"/>
    <dbReference type="EnsemblMetazoa" id="ACON011348-RA">
        <property type="protein sequence ID" value="ACON011348-PA"/>
        <property type="gene ID" value="ACON011348"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009440"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON011348"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037708"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008484">
        <property type="term" value="F:sulfuric ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
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        <property type="entry name" value="ARSB"/>
    </dbReference>
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        <property type="entry name" value="Sulfatase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000917">
        <property type="entry name" value="Sulfatase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10342">
        <property type="entry name" value="ARYLSULFATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10342:SF264">
        <property type="entry name" value="MIP05773P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00884">
        <property type="entry name" value="Sulfatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00523">
        <property type="entry name" value="SULFATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00149">
        <property type="entry name" value="SULFATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026064195" evidence="7">
        <location>
            <begin position="26"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="domain" description="Sulfatase N-terminal" evidence="8">
        <location>
            <begin position="28"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON011348-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="583" mass="66025" checksum="DA3D8435126735E4" modified="2020-08-12" version="1">MKRAVPYLVTLWLLWLGGGASTAHQERPNIVIIVADDLGWNDVSFHGSNQIPTPNIDALAYDGIILNRHYVPPLCTPSRASLMTGKHPMNIGMQDHVIISDEPWGLGLDQKLMPQYFREAGYRTHLVGKWHLGFFRRAYTPTYRGFDSHFGYLGPYIDYWDHSLQMNETSARGLDMRRNTAVNYDANGTYATDLFNDEAVRLIDSHNRSKPLFLVLTHLAPHTGNEDDPLQAPADEIAKFDYIQDPKRRTLAAMVSRIDTGVGRIYRSLERRRMLNNTIILFYADNGAPTLGIHANSGSNYPLRGQKESPWEGAVRGAALIWSRLLPRKGVVSNQWLHVSDWLPTLGHAAGIRIPPNNSPIDGQNQWSTLTSSSSGGRTVVMNNAHNEFTYSSYIKRGWKYVNGTSFKGAYDRWLGQLKDGEQISHEDYYKRLTASQSIGSSMLLTRDDVKRLRSKAMVECSNAQGTVCEPLKRPCLFNIVEDPCERNNQANAYPYILRELETDVNSYRKRSVPSRQQPSDSRADPARHNCTWTWWLEGVGTQSPTHNLQQLFSTFQSFMEYFMALFAKVEAKLLAKVHGGKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W3W8</accession>
    <name>A0A6E8W3W8_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Retinol dehydrogenase 14</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8W3W8"/>
    <dbReference type="EnsemblMetazoa" id="ACON011357-RA">
        <property type="protein sequence ID" value="ACON011357-PA"/>
        <property type="gene ID" value="ACON011357"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004326"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON011357"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037392"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05327">
        <property type="entry name" value="retinol-DH_like_SDR_c_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43157">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS F PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43157:SF66">
        <property type="entry name" value="WW DOMAIN-CONTAINING OXIDOREDUCTASE-LIKE PROTEIN"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="GDHRDH"/>
    </dbReference>
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        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON011357-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="326" mass="35822" checksum="65F0C45449B06FF4" modified="2020-08-12" version="1">MSPLGFNDPLSAVASVVTGVAIVLSLIKYYLYLTCGHFTSSMEGKTVIITGANSGIGKETARDLAKRGARVIMACRNMETAKQAQDEIVKETGNQNVTVLKLDLSSQASVREFAAEVLRTERKLDVLVHNAGFAETFRKTQSVDGIEFTMATNHYGPFLLTHLLIDLLKRSAPSRIVVVASELYRFASVNLNNLNPVGSLPAYLYYVSKCANIMFTRELARRLEGTSVTANCLHPGMIDSGIWRNVPFPLTLPMRVIKSFFKTNVEGAQTSLYLACSEEVQGVSGKYFMDCKEASLSAGISDMEKARKLWDESAKIVKLTESDPKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W3W9</accession>
    <name>A0A6E8W3W9_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_040236005.2">
        <property type="nucleotide sequence ID" value="XM_040380071.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8W3W9"/>
    <dbReference type="EnsemblMetazoa" id="ACON012031-RA">
        <property type="protein sequence ID" value="ACON012031-PA"/>
        <property type="gene ID" value="ACON012031"/>
    </dbReference>
    <dbReference type="GeneID" id="120957728"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002219"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON012031"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035091"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="204"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="277"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="379"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="577"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="707"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="155"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="204"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="240"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="286"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="379"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="579"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="716"/>
            <end position="738"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON012031-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="938" mass="102801" checksum="12E89B8A667201D6" modified="2020-08-12" version="1">MQDFSKDDLGLLSSSVVVSSDKDPLVHTHLQQSHDHPSVHQTHRVDDIKLTNIPDLLEPGDANKLEEPERDLYPWHTGSNIGSRFLRSSDDPLISGGTNDNPHDNELSVPTVSQSMCPSPTTPPPPKHRHPSVILSPELPDATLSPLPTPPKVLADSHTRPPKELQPKRTNSAPRARRKGPLKLRFHHQALPQEYLDHYEATQNSLQRKQEQQHTASQSKPSRQKPAAPQQSGQSASRRAVMERQAALEREERTNESVRSWLQKILELQKEGVPMAPLKDEPIQPAQISPTSESSVGQNGTPPKRIVSYTDLPYMGEITLENSKPRRGRKPKKADICHLIYKNYGTILPGTPSRELGLEKGMLPKGCSKSVASLLERRLTSSDERKQPVGENAQQAALEQPPARGRRKEEPLNLCVRESGGGVVQPAGGETFSVSSSEEDADEVFASSCPTPIITPSDISTDQALAANMKLSLPTLQSSSGSTTETPSTAETPPGYVYWSSGTTNGSLFAHPMSLYSPHPTGEGVGKLPEEHAAKEMRSVVASSNVSSPPISPNGLKNAQNHPLLVPKHISQLLKNEKQSPSNATAGVTGQRSPPAQKRKRSAIFIPPVPAENSHNPATEVSICKFKFTGGAKPCLQEKKILSVDSGGNFRYYSGTGDKSMRGYEFFPRESLQQSSINATSTTGAFLNASGERIACDLPPPSLGLSNDLLQIPEFPTSPQPCGDPTPPPAPGGQPPYRPSAANSAGSASERRRRKTRRATQRESLEKTFKEKGFLIQTQQLQSAEGATYCKFRQLRKFTRYLFRSWKDYLPGELQQSGQEQQEQQHQHAHQLQDGELTSMILPTVITSGPHDDHTIDEESCLTSSTSSVRMETVEGGEHHRSTPPDRTVPASTYHRLRRSRRISSTSVTSQHVHSEALVIDTTTAYRHRHGSPSSVVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W467</accession>
    <name>A0A6E8W467_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="7">DBF4-type domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="EnsemblMetazoa" id="ACON012023-RA">
        <property type="protein sequence ID" value="ACON012023-PA"/>
        <property type="gene ID" value="ACON012023"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005042"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON012023"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042460"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031431">
        <property type="term" value="C:Dbf4-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W489</accession>
    <name>A0A6E8W489_ANOCL</name>
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        <recommendedName>
            <fullName evidence="4 7">Trehalase</fullName>
            <ecNumber evidence="3 7">3.2.1.28</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Alpha-trehalose glucohydrolase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>alpha,alpha-trehalose + H2O = alpha-D-glucose + beta-D-glucose</text>
            <dbReference type="Rhea" id="RHEA:32675"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15903"/>
            <dbReference type="ChEBI" id="CHEBI:16551"/>
            <dbReference type="ChEBI" id="CHEBI:17925"/>
            <dbReference type="EC" id="3.2.1.28"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the glycosyl hydrolase 37 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.28" evidence="3 7"/>
    <dbReference type="RefSeq" id="XP_040231056.2">
        <property type="nucleotide sequence ID" value="XM_040375122.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8W489"/>
    <dbReference type="EnsemblMetazoa" id="ACON012053-RB">
        <property type="protein sequence ID" value="ACON012053-PB"/>
        <property type="gene ID" value="ACON012053"/>
    </dbReference>
    <dbReference type="GeneID" id="120954857"/>
    <dbReference type="KEGG" id="acoz:120954857"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004924"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON012053"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031903"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004555">
        <property type="term" value="F:alpha,alpha-trehalase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005993">
        <property type="term" value="P:trehalose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001661">
        <property type="entry name" value="Glyco_hydro_37"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018232">
        <property type="entry name" value="Glyco_hydro_37_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23403">
        <property type="entry name" value="TREHALASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23403:SF1">
        <property type="entry name" value="TREHALASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01204">
        <property type="entry name" value="Trehalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00744">
        <property type="entry name" value="GLHYDRLASE37"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00927">
        <property type="entry name" value="TREHALASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00928">
        <property type="entry name" value="TREHALASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="546"/>
            <end position="567"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON012053-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="568" mass="65108" checksum="2C50AB2DC1D5D348" modified="2020-08-12" version="1">MSEIYCHGELLKTVQMSEIYPDSKTFVDMKMRKSPNETLDSFHEFMVAQDNSPSKAKLKEWVESNFEKPGAEFENWTPDDWTASPKFLARIKDEDLRGFASELNKIWHSLGRKMTSDVALNPDLYSIIHVDHPVIVPGGRFREFYYWDSYWIVKGLLLSEMYSTTKGMLENFLSIIQRYGFIPNGGRIYYSMRSQPPLLCGMVKSYVDATNDTKFAMDSVETLEREFQFFMNNYVTEVNNHQLATYGYKSSGPRPESYREDVLSAAIFQREEDKQDYYCELKAAAESGMDFSSRWFIKDGTNAGNLTDLKCRSIVAVELNAILYWNALIIAEFYGYRNDIGSIAKKEEYLAKADELKKAINAVLWDEAEGAWFDYDLINKKLRKYFTPTNLSPLWVGCYAREDKELPKRILAYIDRLHLDRYPGGVPNTLQNTNEQWDFPNVWAPMQHMLVMGLDSLDNAEAKELAFSWAQRWVRGNYLTFNKTHSMFEKYDAQELGGHGSGGEYEVQTGFGWTNGAAMDLMNKYADRLTTVDAAEQPEDNGNGALVAYSINTGIIATLLAFFVGVFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WBQ2</accession>
    <name>A0A6E8WBQ2_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TSC-22/Dip/Bun family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8WBQ2"/>
    <dbReference type="EnsemblMetazoa" id="ACON029804-RB">
        <property type="protein sequence ID" value="ACON029804-PB"/>
        <property type="gene ID" value="ACON029804"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005905"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON029804"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037488"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008284">
        <property type="term" value="P:positive regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21936">
        <property type="entry name" value="ZIP_TSC22D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.490:FF:000002">
        <property type="entry name" value="TSC22 domain family, member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.490">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000580">
        <property type="entry name" value="TSC22/Bun"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047862">
        <property type="entry name" value="TSC22/BUN_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46745">
        <property type="entry name" value="TSC22 DOMAIN FAMILY PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46745:SF1">
        <property type="entry name" value="TSC22 DOMAIN FAMILY PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01166">
        <property type="entry name" value="TSC22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58026">
        <property type="entry name" value="Delta-sleep-inducing peptide immunoreactive peptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01289">
        <property type="entry name" value="TSC22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="21"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="324"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="412"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="25"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="324"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON029804-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="507" mass="53459" checksum="F9F67DDCFF882A72" modified="2020-08-12" version="1">MAHVWSKSSRRNQLNTCADEQTTTLSPGSSGLQPQQQSYQLQPMPSAQQLQQAQQQQQQQLQQQRQFIVNPFSDANLSNDSFQNASADRGTMLPKLHPQIAQQPHQMQQLQQPFQQYHAPLYHDNAEKLSKAYSLPPSMGAAAKESLRKDLAQSRLEQQQQQHARKFQPVARAFIVPSSTPGTTNNAPSMTNIANQGNGSNIYTDIALGKTIQTLMKPTPILATAVANRLTGGSLTRSIGALGPCRELPEGGTAGAQEAAGYCRAASSLLGNFTGSNSVSACTVTHPHRCVQDLIKEEIGANRLSKAGSMINMSTDSELFAASGSLSHPASGRSSPAFMHGSTPVGSPPRFVNTLGPAYGAGGGSARVSRAASPSVLHASPGGEHASASGTSAVAIDNKIEQAMDLVKSHLMFAVREEVEVLKERISELMDRINQLEYENNILKANASQETLAQLTTGGSTVAAAAAVAAAAAAVAQQQLTAMPNINVGPTKVPTSSANPANPGSVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WBQ6</accession>
    <name>A0A6E8WBQ6_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Tumor protein D54</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TPD52 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_049462740.1">
        <property type="nucleotide sequence ID" value="XM_049606783.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8WBQ6"/>
    <dbReference type="EnsemblMetazoa" id="ACON029807-RA">
        <property type="protein sequence ID" value="ACON029807-PA"/>
        <property type="gene ID" value="ACON029807"/>
    </dbReference>
    <dbReference type="GeneID" id="120949519"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011523"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON029807"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_043125"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007327">
        <property type="entry name" value="TPD52"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19307">
        <property type="entry name" value="TUMOR PROTEIN D52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19307:SF14">
        <property type="entry name" value="TUMOR PROTEIN D52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04201">
        <property type="entry name" value="TPD52"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58113">
        <property type="entry name" value="Apolipoprotein A-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="170"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="34"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="175"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON029807-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="212" mass="23232" checksum="EAEBA14518F2171D" modified="2020-08-12" version="1">MEDSSYNSGKLSDASPVGSISSAEIANEFSGLSAEEQTAQREEWSQELARVEEEITTLRTVLQSKMRHASELKRKLGITVWKEITDDVSQGIKNVKESNVYQSVETKVGEITTVVTSAPIYQKTESAIKTTAGKTTSVIGGIAGKMTQKLTEMKQSDSFRSFEERVGSAYENVRSKVSSRSSSVQSLSDIQNDERRSSVTTPTAIPEEKPIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WBR0</accession>
    <name>A0A6E8WBR0_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_040224235.2">
        <property type="nucleotide sequence ID" value="XM_040368301.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_040224236.2">
        <property type="nucleotide sequence ID" value="XM_040368302.2"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON029810-RB">
        <property type="protein sequence ID" value="ACON029810-PB"/>
        <property type="gene ID" value="ACON029810"/>
    </dbReference>
    <dbReference type="GeneID" id="120950334"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_000191"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON029810"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041304"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24161">
        <property type="entry name" value="ANK_REP_REGION DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24161:SF124">
        <property type="entry name" value="TRANSIENT RECEPTOR POTENTIAL CHANNEL PYREXIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="13"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="46"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="128"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="161"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="195"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="295"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="407"/>
            <end position="1134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1360"/>
            <end position="1479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="418"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="452"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="474"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="491"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="513"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="531"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="563"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="632"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="652"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="723"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="755"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="786"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="812"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="927"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="989"/>
            <end position="1013"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1055"/>
            <end position="1064"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1115"/>
            <end position="1131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1384"/>
            <end position="1406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1424"/>
            <end position="1436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1468"/>
            <end position="1479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON029810-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1479" mass="161997" checksum="67586A5195730C7B" modified="2020-08-12" version="1">MVAPELIESADEDGFTPLHLAVIQGNLQLVNLLLANGADVNALDNEGHSVVHWATVCGEVEALRAVLAAGADVSTPDINGGSPLHYAAQMCGANYEGKTARASAKLALEILNTLLNHPDTSVEVEDKDGRQPLLWAASAGSAKAVLALIKAGAHVESADKDGLTALHCAASRGHTECIDTLINLCGAHTDQIDSNGCTALHYAVTLGHADATSLLLKLDADPNRQDRKGRTPAHCGCAKGQMETVKILHAKKGNLWLRNAKGDLPVHDAACSGRRQLVQWLIQMKPKHINTTSNDGRTLLHIAAGHDNVDMCKLLLELGADVNLLYRPSSKGAPLTPLDYALAKGYRSTAKYLQMQGGLPANKLRLSGRQQKILPDIDRVEPLKLTEKEELIDLKTSKRYIVYLNSNSDSESQEDRPHRKSRHKRKGSHRGRRTSSCSDTVYLYRDGSNDISRSRSNAELHRSDQRTGKHRRSSSSSSASTSGSSSDGCCRHVRRKHKCYKKCSSKRSHSRDRHKDREREETRDAPLPPDALKEYEFKYAEKKEPGPRKPGERCGKIILKQVHSGSSENDSPANGGGRAGPGLKFSSERRETHLGLPVQLQYGGAGGFDASGGLSDFLDEPTSPRTPPRAEFNIRKESDAKSEGKSSDSSTTKKKKVKGTGKKSKLAKTAKKSDSPSEEEQAKVPAPVTEVVTQAQVHPAPESMSKSTEDDGAATDATYTIEQRTRSDVEGLSETEERSAKGGLPRVTSKPMAETIREEVRDEHAVEMAQQQQQAVRKDRSKLKSAKSDSSKSHSKSESSDKEASKVQQKPPALPLEEPPAAPPAPPPSPPPLAKEPTPEPAPEPKPEAISVPEPKPEPQPAPLPQQLEPLPELVEERTEPTVESQAPQGLEVDRGGGGGPAEDEPLIAEESLPPLVEPEEPPAPTKLEEEQKSTPEETSESSPSKSSEETEPTSVVQEVPEKEEQKPAPTPAVEEPPKVSEVVPPVVEKAKPQEPAKERPKLRRSMESKMESVEEVTAPVLAEPVPPPLDQAKVDQTRGFFVSLDGTAEDDEKKKKKIKKKPRLKEDEQQEQQSSKDQDSGFEPSPQAVRSKPTVFERPTHTATLPRRAAFSMVEERAASSRPEGRKPGDKNAVNMTTVQQSIHRNIRRYYMERKIFQHLLELKSLQIRSTKVNESVLVKRAVDDYHKSTIELGYETGSTLKRYPYTEYSFRNFELFLYDTLKSLQKRETYNFQNISEVYDEAERRLSPDVSRYERALNCTTKTHRCLHATHAYTGIPCAAYIPMMNHHTIPKLGFGPYKSSTSGVGSFFLPKILTHPTDRSCTGGKVALELTHGNSKQVITLPSEKLDSNKRYYVTFTLNPDGTTDGDTRCSSTGDPDPGRAAAAAAAATSAAQPQQQQQQQHQRQSHEQPSDTDAQDNAHPQRSQQQQQHQQPHPSPVAERQRSPAVGPQSQSRALPTVDERRSGGTNSPRGATPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WBR5</accession>
    <name>A0A6E8WBR5_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="3">SUN domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8WBR5"/>
    <dbReference type="EnsemblMetazoa" id="ACON029814-RA">
        <property type="protein sequence ID" value="ACON029814-PA"/>
        <property type="gene ID" value="ACON029814"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_003210"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON029814"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035623"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034993">
        <property type="term" value="C:meiotic nuclear membrane microtubule tethering complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043495">
        <property type="term" value="F:protein-membrane adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045119">
        <property type="entry name" value="SUN1-5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12911:SF8">
        <property type="entry name" value="KLAROID PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12911">
        <property type="entry name" value="SAD1/UNC-84-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="425"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="456"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="138"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="80"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="146"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="173"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON029814-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="577" mass="63680" checksum="A2AC682B4B359305" modified="2020-08-12" version="1">MSQPQNIGDVIETRRRSRSKTPGTMNLRVSSDRELNGSETSPGRKVRKAPTVGMIEEEAAPSPVVAASQGRSTPVRKTRVVTSDYSSEDVSPDRARQAATAVAANAQLAKSMGQLTQSSVTTTTTTMTSTEQTTVVLDGGKEKVTKTQTVTQTSSGSAGKEALSTSPPKKEPASAQKSQMSTTTTTTSTSSSSNSAASTTTRSGKLVGAAATAAAVIRTSTPKNAAQKMAPAKVILTPEELQQHAAYKEYLEAGEYWNKYPKTDYTYSELSPHRREVAPGLVAMPNMSRPSLNKHAERVQTMIQRNPEQEAFIRERYTSARSRLVRSNPYDSHDETDELMQSSKSIGVHRQQRVTIEQRSIVSRFFLSIVTFFFTCVDSVRNVFRRQDEQQMYYTRIEDERGFFSRVYGFVTSFFVNVFKRIYLLISSVLFLDAWLLQTSAANAEQQKIGQKKRRFLLFLLVLLPFLLIGGTLLYLDPSALDTIRASLPSEFPDLPELKLPNLPSLPSLPSLPELPELPTVPLPDVESVKQYVGDRWTDLSDLTGTYFDQLKLVSQNSVDAVRELWGAGGAEEEPSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WC03</accession>
    <name>A0A6E8WC03_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Leucine-rich repeat flightless-interacting protein 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
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            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain>Mali-NIH</strain>
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        <citation type="submission" date="2020-05" db="UniProtKB">
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                <consortium name="EnsemblMetazoa"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
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                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
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                <person name="Galvin B.D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain>Mali-NIH</strain>
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                <consortium name="EnsemblMetazoa"/>
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        <scope>IDENTIFICATION</scope>
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        <name type="scientific">Anopheles coluzzii</name>
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            <taxon>Arthropoda</taxon>
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            <taxon>Insecta</taxon>
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            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
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            <taxon>Anophelinae</taxon>
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    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
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                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
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            <strain>Mali-NIH</strain>
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    <sequence length="8788" mass="978984" checksum="50BA23E53640C25D" modified="2020-08-12" version="1">[encoded data, 8788 characters]</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WC13</accession>
    <name>A0A6E8WC13_ANOCL</name>
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        <submittedName>
            <fullName evidence="5">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
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        <name type="scientific">Anopheles coluzzii</name>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
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    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
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            <dbReference type="DOI" id="10.3390/genes10010062"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain>Mali-NIH</strain>
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        <citation type="submission" date="2020-05" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="5">Ngousso</strain>
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    </evidence>
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    <sequence length="863" mass="93396" checksum="7F39C73D1FBBE34A" modified="2020-08-12" version="1">TSQECLNQYLRPQSLFSEDIEIQPHADDNRLRFDGVYHPSIPEQRCRAPLNAALLAEFENSGSMFSIQSAPVVTAAAVSTTAAPTTTTVTEGPSLLLDDFGIPTQPVAGTTTTTNGAGDERSDTLLRPSSLALDTHYNLAKPHTAVEPQPSTELDEDDNENLLTVSSLTARPLIAKSREIRNKKQKLHIQKQRKPVASDTSEDDDDSPPDGGYGWIIVFGAFSVQFWVAGLVKSYGVLYVEIMENFPNASATTASWIPAILSALCLVLAPLSSALCQRFSCRIVVIIGGVFCGLGLTLSYFATSMYHLLFTFGILTGIGGGLSTTPGIVIVSQYFEKHRALANGITISGTAAGSFVFPMLIERLIHLFGFHGTLLILGGCMLHVCVSGALYRPVEKKQDLVPSKEGTQFSSRSDFRENLGHLSSSEDHLSKRCLEDLFLDDQNRNSLTIDSKFVNITEKTLQESEDELKDILSSAIFLKPISSIRSCSILHSVEDLSTDSTCVYKNRASGYDSKRNSMRYKQQQQQLHQARPQQPPPPQATDQPVGSQTTLGMAEGGGGGEAPLLGPLLLAPNELDKPVSKDVPTKTLAANAGPLPTVMSSNNMCAMVPTKGLAQNNNRGLSKSMIIPTPVCDLDEIFDEDPYTVYNATLLEKISQYLDITLLQDITFILMCLSVALMSVGCPYMLYYLPAYVISAGYTKSEAGYLVAVSAALDLIGRLALGWLSDLDLFDRKKAYIVCILGAGTAVLTIPTSSNAWFVIVISAGCYGLCLGSWYLLVPVLLADLFGTERISSSYGLVRMFQSIGAISVPPLAGLLRDLSGGYEICFYCMGVCMVMGSVPLVAIILRDSIALKDKRLQLDQAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WC29</accession>
    <name>A0A6E8WC29_ANOCL</name>
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        <recommendedName>
            <fullName evidence="2">Regulatory protein zeste</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in transvection phenomena (= synapsis-dependent gene expression), where the synaptic pairing of chromosomes carrying genes with which zeste interacts influences the expression of these genes. Zeste binds to DNA and stimulates transcription from a nearby promoter.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Self-associates forming complexes of several hundred monomers.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040225458.2">
        <property type="nucleotide sequence ID" value="XM_040369524.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8WC29"/>
    <dbReference type="EnsemblMetazoa" id="ACON029484-RA">
        <property type="protein sequence ID" value="ACON029484-PA"/>
        <property type="gene ID" value="ACON029484"/>
    </dbReference>
    <dbReference type="GeneID" id="120951038"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_006864"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON029484"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034198"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028002">
        <property type="entry name" value="Myb_DNA-bind_5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13873">
        <property type="entry name" value="Myb_DNA-bind_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Myb/SANT-like DNA-binding" evidence="8">
        <location>
            <begin position="168"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="101"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="329"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="329"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON029484-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="429" mass="48187" checksum="AD0820B16E4D2004" modified="2020-08-12" version="1">MEKIRTKRAADSEHGKAYPVGVKIVIKEEPLDDDILHGHTQVLREECMVESQTGDGTSDNSDVQDPLLNLAPQTDVKMENLGALGNGIGVAEEIANVAGSSATKRKHSDANELQDDIGTAKTNPHENGCNICPSRELWDARTTKVQYKKMIDIMETAPDIARALFRGSQTQFWEELTTQLNALGPPVKTVGTWKRVWFDYKCAVKKKLLDNKNALAATGYGRRFGTKTLNELEERVATISNLRSIVVGNGRPRYEIDRQIKDNQLICNIIAGNKNITSEDSSEYEKDDDSLPTSLITSRKRKYTDIVVGHSLTQYEIGRQAKDTQRISNVEAENSNITSEDNSEHEKDGTPAVHISRPTRLKRSRKRKNTEAVLQQNKEMLVMMKRFVEIQEKSFKLQQKTNDLLTECTKQLTVCNNLLAKQTNMSTNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WC58</accession>
    <name>A0A6E8WC58_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">C-type lectin domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_049462376.1">
        <property type="nucleotide sequence ID" value="XM_049606419.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8WC58"/>
    <dbReference type="EnsemblMetazoa" id="ACON029110-RB">
        <property type="protein sequence ID" value="ACON029110-PB"/>
        <property type="gene ID" value="ACON029110"/>
    </dbReference>
    <dbReference type="GeneID" id="120953701"/>
    <dbReference type="CTD" id="3772015"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002625"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON029110"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030247"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="121"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON029110-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="211" mass="23691" checksum="6B376F645DB9557F" modified="2020-08-12" version="1">MPAQLTAEHTSPLAALLNGDILDQDRTSARPAMQQQQHDNDAEEEHDEELEHDQEDDEQHHELEEEDDEVNVVGRSSSSANAVAAEEDEVEQEEEEHEHEDEHEQDESDDEDEQAAELHHQQALAAGQDAVGSVLPEDIPVEQKLKQITKEIEQLSGGAGDRELRLDSRQSFLSLSDLIKNIRPAEKVVPQIDSSYANTMRVLGEPLSQRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WDS7</accession>
    <name>A0A6E8WDS7_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ecdysone receptor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">20-hydroxy-ecdysone receptor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">EcRH</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Ecdysteroid receptor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Nuclear receptor subfamily 1 group H member 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Receptor for ecdysone. Binds to ecdysone response elements (ECRES).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 17">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nuclear hormone receptor family. NR1 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8WDS7"/>
    <dbReference type="EnsemblMetazoa" id="ACON029539-RA">
        <property type="protein sequence ID" value="ACON029539-PA"/>
        <property type="gene ID" value="ACON029539"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002516"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON029539"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033826"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090575">
        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035100">
        <property type="term" value="F:ecdysone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004879">
        <property type="term" value="F:nuclear receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035076">
        <property type="term" value="P:ecdysone receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07161">
        <property type="entry name" value="NR_DBD_EcR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06938">
        <property type="entry name" value="NR_LBD_EcR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.565.10:FF:000030">
        <property type="entry name" value="Ecdysone receptor (Isoform A)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000031">
        <property type="entry name" value="Ecdysone receptor A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.565.10">
        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003069">
        <property type="entry name" value="Ecdystd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035500">
        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041889">
        <property type="entry name" value="NR_LBD_EcR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000536">
        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050234">
        <property type="entry name" value="Nuclear_hormone_rcpt_NR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001723">
        <property type="entry name" value="Nuclear_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001628">
        <property type="entry name" value="Znf_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24082:SF507">
        <property type="entry name" value="BILE ACID RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24082">
        <property type="entry name" value="NUCLEAR HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00104">
        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00105">
        <property type="entry name" value="zf-C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01283">
        <property type="entry name" value="ECDYSTEROIDR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00398">
        <property type="entry name" value="STRDHORMONER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00047">
        <property type="entry name" value="STROIDFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00430">
        <property type="entry name" value="HOLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00399">
        <property type="entry name" value="ZnF_C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48508">
        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51843">
        <property type="entry name" value="NR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00031">
        <property type="entry name" value="NUCLEAR_REC_DBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51030">
        <property type="entry name" value="NUCLEAR_REC_DBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 17" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10 17" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 17" id="KW-0804">Transcription</keyword>
    <keyword evidence="7 17" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6 17" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 17" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Nuclear receptor" evidence="19">
        <location>
            <begin position="206"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="NR LBD" evidence="20">
        <location>
            <begin position="359"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="163"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="291"/>
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    <sequence length="738" mass="80775" checksum="6BABD691BCFE8AEA" modified="2020-08-12" version="1">MMKRRWSNNGGFTALRMLDESSSEVTSSSAALVMSPNMTMSPTSLGSPEYNDLELWGAYDENTYNGHSVLSNGNNNLGGGGCGAVAANQLLMNGIMNGGNNLNGMMNGIGLGPGSVGPNGANQASTNQIHQFVGNLINGMNHSQTIIPPLPSIIQNTIMNTPRSESVNSISSGREDLSPSSSLNGYTGDGSEAKKQKKGPTPRQQEELCLVCGDRASGYHYNALTCEGCKGFFRRSVTKNAVYCCKFGHACEMDMYMRRKCQECRLKKCLAVGMRPECVVPENQCAIKRKEKKAQKEKDKVPPNPSTTTVSTTNSSSYKSELLPVLMKCESPPTAAIPLLPEKLLNENRQRNIPLLTANQMAVIYKLIWYQDGYEQPSEEDLKRIMINSPNEEEDPHEIHFRHITEITILTVQLIVEFAKGLPAFTKIPQEDQITLLKACSSEVMMLRMARRYDAETDSILFANNRSYTRDSYKMAGMADTIEDLLHFCRQMYTLTVDNVEYALLTAIVIFSDRPGLEKAELVETIQSYYIDTLRVYILNRHGGDPKCSVTFAKLLSILTELRTLGNQNSEMCFSLKLKNRKLPRFLEEIWDVQDIPPVGLARHQELVAQQQQQQQQQQHQQSDNPNTSSMASTSSTIVVTPMQQSSQLQLQQQQQQQMHSNGVVPTLPVGGGTSVVGGVVTNSITTNGGGAVNGSSSNTGVSSNSSSSSSSSSSNASSNGNSNGLLSTVGMLDHHVV</sequence>
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        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
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                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
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            <dbReference type="PubMed" id="30669388"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <keyword evidence="3" id="KW-0904">Protein phosphatase</keyword>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1478"/>
            <end position="1490"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON029568-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1835" mass="203195" checksum="D54345EC8AE1D1E2" modified="2020-08-12" version="1">MDVSRKARYTDTSYLIFQQLTKGDVLYAFPLVVGLIQRSPRKSAASPSVPSSPKPRTEVNEALVIDSPEESFLGKQKKPNTYKAKDTRKPAGSGIPRRQSKIAEKRKNRRSLELKSVEEVSREEEETKEEDETKVQVCDISGRGGDGTAAAPSPSSSNQPRTISVISSDEPDSVQKDIQVAVDFHHRPNSEVEPFRASAIEANNNDLRCKTANEPDRARAADSDVESVSTLTPSIGPGTDLRGADDAKSEPQQRMAAPGVQIRRKQYPYEPCYLVGSEFSDTSDFTDTASSVASDLDETEYARVELRKKNGREEEQQTINKYNSLPLHTINAQQQQQQQPRPTTNLNHVNNNHYHNYSLQPYYNRQQQQHHQQQQQLQHHSYPVARIDEEPAAQSLRLNYVTERILASTLPARRLQNGSSSPHAPNGTPPVDEHERELINMLEQKHKQNYRILDLEARLVNITLEKLCELCKYIDSWLGSGKEKIVVLQDREDKHRLGTAIAAYLQYQKICGSNIPQASGHSKNSLSPGGTDNGRRTPTWLDLDIYSMQKFLETVAGPLRIPSHRRYIQYFSGLLSGVIKLNSSSFFLKAIRVESPPCLHYRAITVNSEWRSFIKIFEGVRCLFTSDIYVIPITTRHFIYEIKHPLRLRGDILVRCYQIIPNNNKATYEKELIASVQFHTCAITEKEVQFTKGDVDFACEDERFSAEHRMTFCFDTVPNERPMILVFQDPLVRIEPIAENGDLLETINEDESGHTQGPLDGSLYATILKSPKSPTATTHLISPPAEFSNGSSKSLLLLNGNGPPPPVPERSKTPNSIYLSQNGTPRSTPVPFALAPPPAASPAFGDRSVRAVSSASNSVSVNSSYSSYQSASPGVATVPDGGSPGATLATPGGTKARPPSAQQVDGRESVRSPLTVSMDSGISSSGPVNRRVQGSSVSPSSFPSPQASPQDDRHRELDDLLSDMMLTVQGIPDVGQKADQTDHHQSQQPQQQQQHQHHHHHHHHHHQNPNDHFVNTNTDTIKRSHSAQLPQREDARSRTPLTAVSDYSPATAAAAAAKERELLMYETSSTTTTLTPPPSESGRETPLLSGPSYAAAAAAGTIRERELQNINNNNLTPAERELIMSVQRHQHQSLAYPRPARSTTGASERFTSDDDDDLLVHGGTGSVAQIPYHAREDSRPFTYGNIPATGTPQQPPAATMLKMQSGLSSPSMVRKALGTPTASRKTAGTLPRNDFEEMLRERREKVLSEKYTIGDQSPGGGMMVSDTINNNSSGDGRWTYQQHHTVKTVTTQGGGGAGTPNGYPSHEPLKRSNTMDGSFGRQFSNEGISGQSWLQLQQQKLRARREQQRREHSNSFSYNYGSPAFPTAGENAYSTTHRRSQTLSPVRNERNYHTLTTTRTHSTERPFVAVQRAHDNAKLQTIGAREESFSSYRSETEPDNSPMGGSPRPETPAFPVTPRTPYGLSNGTSSPALPPKSPTSQRRYNSAWSLRSQKSSVSTYDVSKDLFSGNQQRTQELVNQNETLSCYTSRRNSTTSNANSEPQEVAPQFVKFARDSSKYWYKPNISREEAIALLRNAAPGTFIVRDSTTFANAYGLVVKVNHPPPGVQYTGPNSEELVRHFLVEPTIRGVRLKGCANEPVFTSLSALVYQHSITPLALPCRLIIPDMDLQQMEHQTPAQQQLLQQGAACNVLYLFTCDTESLTGPQAIRKAVSSLLALRPLPKPTQVHFKASLQGITLTDNTRQLFFRRHYPSNNVSFCALDPDDRRWSIQSTTGDIPASKRMFAFVAKRSPSSADNQCHVFCELEPTQPAAAIIQFANKVLSGTSQQPAVTRAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WDX7</accession>
    <name>A0A6E8WDX7_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Fibronectin type-III domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_040219638.2">
        <property type="nucleotide sequence ID" value="XM_040363704.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8WDX7"/>
    <dbReference type="EnsemblMetazoa" id="ACON029605-RA">
        <property type="protein sequence ID" value="ACON029605-PA"/>
        <property type="gene ID" value="ACON029605"/>
    </dbReference>
    <dbReference type="GeneID" id="120947892"/>
    <dbReference type="KEGG" id="acoz:120947892"/>
    <dbReference type="CTD" id="40861"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004078"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON029605"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042381"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045597">
        <property type="term" value="P:positive regulation of cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050941">
        <property type="entry name" value="CCN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001007">
        <property type="entry name" value="VWF_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11348">
        <property type="entry name" value="CONNECTIVE TISSUE GROWTH FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11348:SF34">
        <property type="entry name" value="EPIDERMAL CELL SURFACE RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00214">
        <property type="entry name" value="VWC"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026070503" evidence="4">
        <location>
            <begin position="35"/>
            <end position="1104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="1043"/>
            <end position="1068"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="5">
        <location>
            <begin position="684"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="5">
        <location>
            <begin position="818"/>
            <end position="917"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="5">
        <location>
            <begin position="922"/>
            <end position="1019"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="63"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="241"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="482"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="102"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="482"/>
            <end position="495"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON029605-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1104" mass="119098" checksum="E6633051922BFF96" modified="2020-08-12" version="1">MVRKKTSKMDTANGRLVLLAAAAVCLMFCWPALAQDAEAPTTTSAGSDEPAPAATSTIANTIEPSEPTTAGSPLEVLPLATNPPTADSGESIATDQPAPVDGTTTTQQAPIDTSAPSTEGTTAQPDTSTSPEVKTVQEDADRTTTTIAPTTVSDVSTAPPTVAAPKPVILHVKSPEEEANDRAGDRPPSILNHFLPSGESSSEELLKMSDVRASAEVRGRAISFGPAEPINFVTAPNLVTTVPPPPGRAPAADQSTKPPAFDDLSDVSMESTDGTEPGLEPADGKSMEMAMMTPGECIFNGIEYKTNATVDKDCDERCHCEEDGKWRCEPRCGRPFIKRGKTALAAGCYENPSKTDDCCATLICPSERLTDGRVEPTPVSEEPTTTIPTTTTTTARAIVTSCIYKNRSYGINERMEVGCEQICTCTSDGEMECAPRCAPLNATRSEQCVVLPDPHDPCCKREFCDVTLDDHEQNSGVMMMMAPASSSSSPSTTAAGPAEQADTSAQPAGCEFKGRPYRLHDQFHDECNALCLCTEAGVHCAKIECPSEFGLDVLDPHCLKWGPEPATFRAIAPRCCPERMRCIDNGTCEYKGQFFDNWSDIPSNLTGCEQHCYCDTGIVECRPACPPVPAAPPSHLPCGSRNARLMPIPGEECCKHWACTPNADNNGIASGPGGHAGGLAFSGLQVDLKVLALDAIDPNTVRVIFLVPQVYVGLHGRVELRYTRTRNNDTSTWQSQVFAPPDDLIATPQLEFELPGLNANTEYRVKITLILRDVNSQPSSQVYTVRTPSDRVITPPSIGIGTGHFPQMSHPTNFLKEIIQDPELRAAEVNSTWAKIAWRKLDDEELDYVDGIQLRYREIDSIVYHATPLIHRALSEYTVEGLQPQTRYEVGIFFIPFPGHGEEVRAGEMLPITTAERVDVYGFEVVVNASKIKATSVEVSWSGVPYPEDKYINIYRAIYQSDSGKEDSSVFKVAKHDSTTGTLIADLKPGTRYRLWLEMYLTNGSIKKSNVVNFITKPGGPAVPGKTDKLAAAGAAGAVNGDYYGPLVVVAVIAALAVMSTLILLLILTRRRVQSATITPPRKNDAAYDNPSYKVEIQQETMNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WDX9</accession>
    <name>A0A6E8WDX9_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="8">C2H2-type domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="EnsemblMetazoa" id="ACON029608-RA">
        <property type="protein sequence ID" value="ACON029608-PA"/>
        <property type="gene ID" value="ACON029608"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011410"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON029608"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041904"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050688">
        <property type="entry name" value="Zinc_finger/UBP_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24403:SF67">
        <property type="entry name" value="FI01116P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24403">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="847"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="149"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="218"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="326"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="439"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="547"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1038"/>
            <end position="1081"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="152"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="218"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="256"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="281"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="326"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="370"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="389"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="555"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="577"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="629"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1038"/>
            <end position="1064"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON029608-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1327" mass="150585" checksum="4F65FF0AB19C149C" modified="2020-08-12" version="1">MGAIARRPRSARPVITMDDSRDTLNNSLREEVQQKLRQAKDLLRRVRDKDFCSGMKNSLVRIEPIILNSTIRLKTSTLLEVRKILEKGVQKTTSKLVLERYNANMPAWDTTDLLRLSGQSSRPNSHQGYSSMGNQERLALKYPRRKIGVAGHSSTSNDPGNTASRSSSPLFRRRHEVFPSQPIPTTYAEHKRLSQNQQFQQPKSARDNQFVSNINNAPVETDNQSINKIPDSTSHSPTHVSVPNETRYDTDKSKSLARNTESSFDTGVPTSANSPTSSHAVHVEKRNSEFSDHRDFKDPNSVDSRKTSCANDVSIERRNSEYSNLYKDKKYIKERKDSKERKDSKDRKDSKERKNSTERQDSKERKNSKESTSADSTTSNHVDADNRPQKVQSDTHTNGVNRDSFEITTTSCSPELHQQTDAMEDVPEDPLKETAKVTNALAQKSSTSKPSKPKSKGHSRELHDLLSYTTEIMPKIVNRRKSISNETAVINAQLRAQFNITKELVVALTRVQTGVVAHRTRSKSIDSTKFLESRKVVEQITPETNTTARKIQISDTKEKKSVDQRKHKKSPEKKKSASSTSNSSSSSRMLRQTARKSSSKPETSASKKKTVEKKLVIESATTEIDKTPVKPKHISKKREIRSRSPSPSRPKRLRSSQNNSIVACKEEFVNKSSEETEDEESSIILSNLILRENSKPNNDPRVHCHSDYMEECCLCSYKGENMVQHYVYNHPKKSVYISRVAPEQADLIKADPLGINGEKITRQSFRFGSQEVIKFRCYFCDLYISESQELWIDHISGHTGEYRYKCVLCPTVARSSIERVMHHAVCSNPSIQLWHKYSFENNHLYGYMCDECNFVQVLYENMVRHLRDDHAGDGTQQNKIIRFSLVNFSLQDNEADLDCGIVKLEPILDDTKQSIDEQLRREMEFMFGKDPPSIVDTEQFEDCIEEKIHFRPTNIVVKQEKLIEPQQPILSPPTTENHEARVNNCVPVNGNEIHEVKSIIRLKRLPGDILSIPKENSLPKISSNAMISSDNFPKITSNSTLSSDNFPKTNSNSTVPSDKSSSARIGNRDDRPRSTFITSSTSAVNPTKLAARLPNGNYQPILPHPLATVVTGVVQTRPPVRILNGHKISKPLSVSQPPVVTTKHIRLPPTVINGLQPMHQPSSPPMAALKPWNGLRNAGKLSLHKLTTSYLLAEFKCTVYDCGFHSETLETMKEHFLIHQKPSNQHSITRFSPWLECWYCRNVATSSDNLLEHVQMMHKHCGYQCDQCCYRSRDPNSVVVHQRKYHNELFQNAKILCVPGRQKPYTPMDDDAIRIEMKTNVKGLHCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9I3AJF0</accession>
    <name>A0A9I3AJF0_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Myosin motor domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2023-03" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9I3AJF0"/>
    <dbReference type="EnsemblMetazoa" id="ACON004403-RB">
        <property type="protein sequence ID" value="ACON004403-PB"/>
        <property type="gene ID" value="ACON004403"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_038668"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005902">
        <property type="term" value="C:microvillus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030048">
        <property type="term" value="P:actin filament-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010926">
        <property type="entry name" value="Myosin_TH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140">
        <property type="entry name" value="MYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140:SF802">
        <property type="entry name" value="UNCONVENTIONAL MYOSIN-IB ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06017">
        <property type="entry name" value="Myosin_TH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51757">
        <property type="entry name" value="TH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="2 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 6" id="KW-0505">Motor protein</keyword>
    <keyword evidence="3 6" id="KW-0518">Myosin</keyword>
    <keyword evidence="1 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myosin motor" evidence="8">
        <location>
            <begin position="19"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="domain" description="TH1" evidence="9">
        <location>
            <begin position="1050"/>
            <end position="1235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="143"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="238"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="298"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="6">
        <location>
            <begin position="781"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="146"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="301"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="112"/>
            <end position="119"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004403-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1235" mass="142006" checksum="EE16B53351B8C3AE" modified="2023-06-28" version="1">MRKSAKTDAGKIPNMEQEIGSWDSVLLENLSEESFINNLHQRYKRDLIYTYIGTFLVALNPYKQLQIYNPDQVRKYATKSLFQLPPHIFALANSAHQFLLNYNEDQCIIMAGECGSGKTESSHMIVHFLTQLSEMRRSRAPIFSLRGSNPNSRQSTPKHSTISRVGSSFESAAPAGGRLDSIMKYNSSRQQKCSHEKTVEFDLISHHKSTENLPAMVNYGFMPVATMPKCLKHSNQSISRSTEGNEFPTKSTLKSATTMGSCPKHNCTSSHAELNRMCGGASPSPSLQKRSNLMRCCHQNSNNSTRSIHKSSSSIALTHDHGTTSPSTFHRRQILKSISKEVYIRDLELQKMRERVAQAEIFLEAMGNAVTVKNMNSSRFGKFFDIEFDYKGDPVGGHLTLYMLEKSRVTTRASGERNFHIFYQLLSGADIQLLKSLKLQRNIDKYELLKNTFANEDDRTNFTFTKRSLEILGFGQDEIFSIFTILAVVLKLGNLTFIPTTNIDGSEGCEISNEYELDEIAQLLQLDNQMLFNCLTRLGDNWAQLEPDGTEIDASYASRIKFTLCRTLYGRLFTWIVSRVNDALKLKTSGTVGSRGKTIGLLDFYGFEALEKNTFDQFAINYCNERLQQHFIKSVLKHQQDLYVNEGLDWIRIDFFDNAPICELIDKPCFGILHLLDEPQVVNDGLLLTRLHQCCAGHTNFLARDASLPSNCFQVRHFEGPVVYTTNGFIEKNLDLLPRHISSCLFQSDLLIASCLFPEGNPKRHSNRKPSSLSNNLRTSLQTLLKLLEQRSNHYIFCIKPNELKQAKMFELGLVQHQVRYLCLMPLINLWRNGHCFNMVHARFLARYKLLCQYTWPHFTGTIVDGVALIIRSVPLPGAEFTIGRKKVFIRSPRTVYELDEFRKVRLNELAILIQKTYRCYTKRKRFLTLRHSQLIIASYWRTWREREKIRLVEFRKQALWAVQVIGRFFVLLKTREFLLTLLLRLPHDNMSPICHEWPSAPTFLAETSQLLRTIFHRWRCYKYRKSFDQPARNRMREKVTASIIFKDRKVSYPRSVAHPFHGDYIRLRQNIQWKRVSCEHNDQYVVFADIINKIARSSGKFIPILLVISTNSMLLLDQKTMQIKYRIPASEIYRMSLSPYFDDIAVIHIRASEIGKKKGDFVFQTAHSIEIVTKLFLVIQNATAKSPEVTISTDFEANFGGQTVIFTFKCGGLVDNPYNQTRVTRKGNRMEVIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9I3ANM0</accession>
    <name>A0A9I3ANM0_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Pleiotrophin/Midkine C-terminal domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2023-03" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the pleiotrophin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040220190.1">
        <property type="nucleotide sequence ID" value="XM_040364256.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9I3ANM0"/>
    <dbReference type="EnsemblMetazoa" id="ACON005674-RB">
        <property type="protein sequence ID" value="ACON005674-PB"/>
        <property type="gene ID" value="ACON005674"/>
    </dbReference>
    <dbReference type="GeneID" id="120948184"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_029576"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048332">
        <property type="term" value="P:mesoderm morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.90.10:FF:000001">
        <property type="entry name" value="Pleiotrophin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.90.10">
        <property type="entry name" value="Heparin-binding Growth Factor, Midkine, Chain A- C-terminal Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020090">
        <property type="entry name" value="PTN/MK_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038130">
        <property type="entry name" value="PTN/MK_C_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21050">
        <property type="entry name" value="MIDKINE AND PLEIOTROPHIN 1, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21050:SF1">
        <property type="entry name" value="MIDKINE AND PLEIOTROPHIN 1, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01091">
        <property type="entry name" value="PTN_MK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039933982" evidence="7">
        <location>
            <begin position="24"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="Pleiotrophin/Midkine C-terminal" evidence="8">
        <location>
            <begin position="124"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="165"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="171"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005674-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="194" mass="21218" checksum="868413462C5C0ED4" modified="2023-06-28" version="1">MNILVISFLAVVSLTTFPAGTYGTPAASSGAHDPKKSVAAAEAALPVNDNGLKEIWQEDDREVLIRNERGTKNGGSAAADSQCRYTKGPWTECDAKSNTRSRTLSLKKGESSCVQTRTIQKKCKKACRYDKGAWSDCDNNGQMSRTDSLKQTSDATCQTTRVVNKNCNQGKSKDKQNKPPKAEKKEKGVRKNRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9I3B222</accession>
    <name>A0A9I3B222_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="15">FZ domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2023-03" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9I3B222"/>
    <dbReference type="EnsemblMetazoa" id="ACON010706-RB">
        <property type="protein sequence ID" value="ACON010706-PB"/>
        <property type="gene ID" value="ACON010706"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030314"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07066">
        <property type="entry name" value="CRD_FZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000034">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.2000.10">
        <property type="entry name" value="Frizzled cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020067">
        <property type="entry name" value="Frizzled_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036790">
        <property type="entry name" value="Frizzled_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050685">
        <property type="entry name" value="LDLR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24270:SF57">
        <property type="entry name" value="FI24007P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24270">
        <property type="entry name" value="LOW-DENSITY LIPOPROTEIN RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01392">
        <property type="entry name" value="Fz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00261">
        <property type="entry name" value="LDLRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00063">
        <property type="entry name" value="FRI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63501">
        <property type="entry name" value="Frizzled cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50038">
        <property type="entry name" value="FZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="2 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="118"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="FZ" evidence="14">
        <location>
            <begin position="368"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="227"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="290"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="598"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="648"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="230"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="599"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="655"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="413"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="488"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="495"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="507"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="545"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="730"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="737"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="749"/>
            <end position="764"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010706-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="768" mass="85044" checksum="298E6CFA38B0EFAB" modified="2023-06-28" version="1">MHECLTESTNSPKWRIIQGSSHNPSAHSPVDLDVKACVAWCRSGTPYSGYTAASTDCANTLPSSPSPTSLGSIHNRQSFNFWKAGSQTPLSASSSSSSFFRPTQRCLLYKILKVIWRYIKWPLGLLMICGLLGLVVYFLVVDHDIMSSGLLGEDGYEVDENLSIDFNDFTSTNDLHFDHRFTGSIAKESKPKDAQGPGRTVDTKFSFGLDNDLLAVFGTNGKKPTYDWPTTASTTTGLSGTERQRTESASEELPALVVKVTDAPIRKTSTISLKDYTKARVVFDDEDFLDADDGQSRTQSAPAHPEGTTRAYELGHSTDSALPATQKNVTVPSATTDSSLYRTKVSPTLPILSRFEITTEGLKRFNISEEGICQSTSLPLCRGVLPYDLTVSNNRQLSPLEYDHFQYLINSKCSVRVAEFVCLVLEPECRPTRMGTLTPCKRICKSILEPCAHIIASSEALTATFDCDSYPDSDDRNVCEDPTRLGHCYANEFRCADSSCIPLQWKCDNIKDCHGGEDESDCKQCERDEYRCMSNDKCIPDKWRCDQYEDCPDASDELDCYYDEPTTYPTAYGSQREYPFVQVQVPNSPNGRPYITITNEDRSQAERHRTSQEAGSAGAREYANYGTSEEDEAGDKYEPTAAENAPFVNRHRNQTDAGNGTVEQTTTVRSSEKPVEDQTEAETSRTVSIPKSLVNFQDSKEIMMTSDSENDFKISSTSRDFLTTAHVSPCPEGELRCVSGICISISQLCDRVTDCADGADETGCNYKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9I3BE86</accession>
    <name>A0A9I3BE86_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Laminin G domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2023-03" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_049463931.1">
        <property type="nucleotide sequence ID" value="XM_049607974.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9I3BE86"/>
    <dbReference type="EnsemblMetazoa" id="ACON030732-RB">
        <property type="protein sequence ID" value="ACON030732-PB"/>
        <property type="gene ID" value="ACON030732"/>
    </dbReference>
    <dbReference type="GeneID" id="120947924"/>
    <dbReference type="CTD" id="104327"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041819"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005587">
        <property type="term" value="C:collagen type IV trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030020">
        <property type="term" value="F:extracellular matrix structural constituent conferring tensile strength"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00247">
        <property type="entry name" value="Endostatin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1620.70:FF:000001">
        <property type="entry name" value="Multiplexin collagen isoform Ap3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1620.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050149">
        <property type="entry name" value="Collagen_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010515">
        <property type="entry name" value="Collagenase_NC10/endostatin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045463">
        <property type="entry name" value="XV/XVIII_trimerization_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24023:SF1112">
        <property type="entry name" value="COL_CUTICLE_N DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24023">
        <property type="entry name" value="COLLAGEN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20010">
        <property type="entry name" value="Collagen_trimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06482">
        <property type="entry name" value="Endostatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0176">Collagen</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039905984" evidence="3">
        <location>
            <begin position="21"/>
            <end position="1142"/>
        </location>
    </feature>
    <feature type="domain" description="Collagen type XV/XVIII trimerization" evidence="5">
        <location>
            <begin position="858"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="domain" description="Collagenase NC10/endostatin" evidence="4">
        <location>
            <begin position="943"/>
            <end position="1108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="251"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="378"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="816"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="283"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="302"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="322"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="445"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="482"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="623"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="654"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="672"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="714"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="749"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="824"/>
            <end position="834"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF06482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF20010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON030732-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1142" mass="120038" checksum="36399C2C59A9A896" modified="2023-06-28" version="1">MKWLYGTVVTLICIAHRVTSSELNIFGGQGIRDALAERDLMGAIEIPLQNGVKFVDGLDGFPAFGVTSEADLKSPYKMILSDHLQDFALIATVRPQSSSGGWVFSVVNSLDTVVQLGLLLEPTAGGDQWNATLYYTDAKKERISQPLASFQVPYGKSWMKMIFKVLPDQVVFYYNCLEAGVVPVKKEPRKLVFDSASTVYIGQAGPVLKQKFEGTFLFLKIYGYPEIVKTHCNRTSLPIDEELESTDEFGDDFNSEMVYDQSGDDDGFNEPPMISPPPPEYGYRLKGDKGERGTKGESIRGPPGPPGPQGPPGPPGPPGVAGPKGGGAFDGDGSGDELKRLSQLYDTLPNRKHGGQCFCNASLIIEELKMDSKLREYLRGPQGMPGKEGKTGAPGLTGVTGPQGERGASGPKGDKGDRGDQGAAGPEGLHGSKGEPGLDGAPGVQGPPGPPGPPGLPENYDESMLGAPIQGMRGGSPGLKGEPGEKGEIGFPGEKGEHGTKGDRGDPGLTGAKGERGHQGAHGQTGPKGPPGIPGIPGLPGQTGASGPKGEKGNTGESGPPGPPGPPGMVMHTEGGRNGTTDQCQCQPGPPGPPGARGPSGYDGAPGLPGETGLPGHPGLPGDKGERGLPGPKGEKGPEFIINENAAFNSSRANKGEKGERGQRGRRGKTGPPGPIGPPGKPGGMGETWPGRPGPKGDQGPKGEKGDSMAMRGLKGDKGERGMNGRDGLPGPPGLPAASGDGGVQYIPMPGPPGPPGPPGQPGPPGLSIVGEKGEPGMDSRSPFYSDSQHGFYGRPGGRSSLDELKALRELKHHKDYEDSTLGPPGPPGPPGPAGRPLHDSDEIPNSYGANVRIVPGAVTFQNAETMSKMSSTTPVGTLAYIIDEEALLVRVNKGWQYIALGTLVPIATVPPPTTTIVPPQRADLQASNLIHNLPQPGDGSVLRMAALNEPYSGDMQGIRGADFACYRQARRAGLLGTFRAFLSSRIQNLDSIVRIADRELPVVNTRGDVLFNSWNGIFNGQGGFFSQAPRIYSFSGKNVLTDMAWPQKLVWHGSSAHGERAIDTYCDAWHSQTPDKVGLASSLLGNKLLDQERYSCDNRFVVLCVEAVPQDRRRKRRDTTSQHEFANEKEYSQYLQSISAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABM2A8D0</accession>
    <name>A0ABM2A8D0_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Progestin and adipoQ receptor family member III</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ADIPOR family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_049461853.1">
        <property type="nucleotide sequence ID" value="XM_049605896.1"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON000144.R226">
        <property type="protein sequence ID" value="ACON000144.P226"/>
        <property type="gene ID" value="ACON000144"/>
    </dbReference>
    <dbReference type="GeneID" id="120948043"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004254">
        <property type="entry name" value="AdipoR/HlyIII-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20855">
        <property type="entry name" value="ADIPOR/PROGESTIN RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20855:SF15">
        <property type="entry name" value="PROGESTIN AND ADIPOQ RECEPTOR FAMILY MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03006">
        <property type="entry name" value="HlyIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="127"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="158"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="192"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="224"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="253"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="284"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000144.P226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="359" mass="40436" checksum="8511E2CB2289C7C9" modified="2025-10-08" version="1">MNEWNELLKAELLPPSGSFRTDGDAPGSIKCAGLFQGSVAAPGDCGLYHRHPQKGIANPPVLEDATEKHGSSSALAPTSWREKVVAYDEAPEHLRFNPFIRTGYRTYLSARLCLESMFWWTNETVNIWSHILGLCVFLILAYYDTAMLQIQASTTDKVIVGMLLLSFQLCMIFSSIYHTFSCHSADSYDRLLAFDLFGIALSLLAIFMSGIYYAFWCNQPLRDFYMITIGVIFTAAMALQIPQLNVHSNVKMLAFVAWAAYGVVPTLHWYFVMGGTESTMVKIFIPRVAVMYALSGFAFLIYVAKIPERWCIGWFDCIGHSHNLWHLIVLAALCYWHNSGMKYVEFRMTHGCSAGVQYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABM2A8G8</accession>
    <name>A0ABM2A8G8_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Postsynaptic cell membrane</location>
            <topology evidence="16">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040221190.1">
        <property type="nucleotide sequence ID" value="XM_040365256.2"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON000138.R168">
        <property type="protein sequence ID" value="ACON000138.P168"/>
        <property type="gene ID" value="ACON000138"/>
    </dbReference>
    <dbReference type="GeneID" id="120948640"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd19031">
        <property type="entry name" value="LGIC_ECD_nAChR_proto_alpha-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19064">
        <property type="entry name" value="LGIC_TM_nAChR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002394">
        <property type="entry name" value="Nicotinic_acetylcholine_rcpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00860">
        <property type="entry name" value="LIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00254">
        <property type="entry name" value="NICOTINICR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15 17" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 17" id="KW-0406">Ion transport</keyword>
    <keyword evidence="14" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="9 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="17" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0770">Synapse</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 17" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044963769" evidence="17">
        <location>
            <begin position="28"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="248"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="283"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="313"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="509"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="18">
        <location>
            <begin position="33"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="19">
        <location>
            <begin position="254"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000138.P168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="557" mass="63305" checksum="06692499AA897127" modified="2025-10-08" version="1">MRAIMPTSGETLRAWLLSALVVHGAVAGNPDAKRLYDDLLSNYNKLVRPVVNTSDVLRVCIKLKLSQLIDVNLKNQIMTTNLWVEQSWYDYKLRWEPKEYGGVQMLHVPSDHIWRPDIVLYNNADGHYEVTLMTKATVYNNGMVIWQPPAVYKSSCSIDVEYFPYDVQTCVLKLGSWTYDGFKVDLRHMDEKSGSNIVDVGVDLSEFYMSVEWDILEVPAVRNEKFYTCCDEPYLDITFNITMRRKTLFYTVNIIIPCMGISFLTVLTFYLPSDSGEKVTLSISILISLHVFFLLVVEIIPPTSLVVPLLGKYLIFAMILVSISICVTVVVLNVHFRSPQTHRMAPWVKTFFIDFLPRFLFMKRPPYIENHRKLLSKDLHACFYPYYSTTTLNRIARFTNRAPSKEDLSPSSLSGTGPFGGSCQIHGPIQLPHSESEELSMSTAADTAVPSGIKSPAFKQPAFSHSVCSTEVHRSCFCVRFIAEHTKMLEDSTKVKEDWKYVAMVLDRLFLWIFTLAVLAGTAGIILQAPTLYDDRIPIDKTFDELATSTVVRCPPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-10-08" modified="2026-01-28" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABM2AA82</accession>
    <name>A0ABM2AA82_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Single-minded</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_040235070.2">
        <property type="nucleotide sequence ID" value="XM_040379136.2"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON000773.R85">
        <property type="protein sequence ID" value="ACON000773.P85"/>
        <property type="gene ID" value="ACON000773"/>
    </dbReference>
    <dbReference type="GeneID" id="120957115"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001610">
        <property type="entry name" value="PAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013655">
        <property type="entry name" value="PAS_fold_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23043">
        <property type="entry name" value="HYPOXIA-INDUCIBLE FACTOR 1 ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23043:SF36">
        <property type="entry name" value="PROTEIN SINGLE-MINDED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23171">
        <property type="entry name" value="bHLH_HIF1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08447">
        <property type="entry name" value="PAS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00086">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="9">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="8">
        <location>
            <begin position="78"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="8">
        <location>
            <begin position="388"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="148"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="502"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="672"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="735"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="806"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="904"/>
            <end position="977"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1029"/>
            <end position="1116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1132"/>
            <end position="1161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="223"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="240"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="256"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="282"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="502"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="673"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="741"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="760"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="770"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="845"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="904"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="960"/>
            <end position="977"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1029"/>
            <end position="1056"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1065"/>
            <end position="1084"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON000773.P85"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1161" mass="123685" checksum="2A5DB6FEA25FF6BD" modified="2025-10-08" version="1">MKEKSKNAARSRREKENAEFLELAKLLPLPSAITSQLDKASIIRLTTSYLKMRAVFPEGLGDAWGTQHIPNNPRDLAIKELGSHLLQTLDGFIFVVAPDGKIMYISETASVHLGLSQVELTGNSIYEYIHNYDQDEMTSVLSLQPNMYVGPPAGGNGTEPSPGTGPLLASTPSHSSLAADAGGPTGTTDSPISPFASLLHHQHHHHHPGGGESPPQSLTSSGGSIGTTLGGPLGQSPNDMQAGMHHQQQQQQQHMGGNGGHSPLGAVYGSGPGGYGGMPQPPHHHHNHHHGHYHHHHHHSYGQHQTIEIERTFFLRMKCVLAKRNAGLTTSGYKVIHCSGYLKARIYPGDATYGEGHSCIQNLGLVAVGHSLPPSAITEIKLYQNMFMFRASMDLKLIFLDAKVAQLTGYEPQDLIEKTLYQYVHAADILHMRYSHQVLMYKGQVTTKYYRFLTKGGGWAWVQSYATVVHNTRSSRPHCIVSVNYVLSDQEAQDLLLNEVQQPQHSAPTQHQPSPSVKPEAGGGGGGGVAASNKERSLSEPATALAATMTPVSGLASGVPAHMSPIGGTVVVGGNNGTARRGASVQPPHQASMSYGLLGKELQHQDHLQQQQQQQQQQHQHNTFLQLANLDDDASLGPIPGAPSACLVTHPGSTGLPPSTGQDEYELQMQYGPGTTHQQEPNTGSTGYCMNAAPTGGLLEGHGAGPHPGTDGSDAFLEPYYDQFYGGYDSRQDPISMRPYSASSNSCSSSEAEGVLGHQQQHHQQHHHQQQQQHHQQHPLQQQPPPPSYGSMSMAGAGVLRQHVTTGDLGNTYGDHHQLDFVPSNGHQQQQHHHHQLQQLDQHTGHTNGGNSSFLYDASPATMDGSTSSASPFDGIAQPFELHHLPTTHGHFRQQQHADLQQQLGESPLVSSTSSSSSSALLGGSPARRPAPDRDESVVSGAEPGASKQPKLANHHQHQHQLANGTVSSNGTTTGSATALQATNGAASSNHLRGAGNGSIIISTTDTNQGADTNGVVTVSINLNHLHSSTGGVSSSSGSTSGGPSSISNSHLLSTSANDHNGAINNNNNNNNNNNNNTNSNNNNLHSLEKKGGPVPNRNSQPVRSQPPHYTADSHYDLPHYTSVIVESTANGSTAAPNNGANSVTGKLSSNEYISQQQPCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8UZH1</accession>
    <name>A0A6E8UZH1_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="12">C2H2-type domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8UZH1"/>
    <dbReference type="EnsemblMetazoa" id="ACON001083-RB">
        <property type="protein sequence ID" value="ACON001083-PB"/>
        <property type="gene ID" value="ACON001083"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012922"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001083"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035713"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002496">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001732">
        <property type="entry name" value="Zgc:162936"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050717">
        <property type="entry name" value="C2H2-ZF_Transcription_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14196">
        <property type="entry name" value="ODD-SKIPPED - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14196:SF0">
        <property type="entry name" value="PROTEIN BOWEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="272"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="300"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="31"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="128"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="31"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="64"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="128"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001083-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="348" mass="37008" checksum="E61D934AE2705D43" modified="2020-08-12" version="1">MTPAQKNAYLEAHMAVQQQMAQAALQLQHQHHQQQLQQQHLQQQHSLSYNRAAKKTKISHDPHQLLQQGSHQSQQHQHQPGSSPSAHPNPTVSHYINTIKVPQINAGTHASASNAVINTAAPAGTVTSTTTATSSARITSTGSTSGNSIGRDGGGCSSGSSGSSTFTVPDDVGMGFEGGVRVLQSLGNWSPEIPPNIPRPNLIPFTEPYAEGGSMHPATRLKALQQVHQSSAAAVAAATVATSSALRSKASSVKSKASGTTADPTASSKPVFECSVCGKGLARKDKLTIHMRIHTGEKPYVCEVCDRAFARRDKLVIHMNKFKHITPTNIAPLGKRQNRSFTIGLNPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V382</accession>
    <name>A0A6E8V382_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8V382"/>
    <dbReference type="EnsemblMetazoa" id="ACON001083-RA">
        <property type="protein sequence ID" value="ACON001083-PA"/>
        <property type="gene ID" value="ACON001083"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012922"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001083"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035713"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="128"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="31"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="64"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="128"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001083-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="224" mass="23843" checksum="5F47119BEBC4934A" modified="2020-08-12" version="1">MTPAQKNAYLEAHMAVQQQMAQAALQLQHQHHQQQLQQQHLQQQHSLSYNRAAKKTKISHDPHQLLQQGSHQSQQHQHQPGSSPSAHPNPTVSHYINTIKVPQINAGTHASASNAVINTAAPAGTVTSTTTATSSARITSTGSTSGNSIGRDGGGCSSGSSGSSTFTVPDDVGMGFEGGVRVLQSLGNWYIVLSDTARLHLTSSSLSFSFTTTCNLLVILFVER</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V387</accession>
    <name>A0A6E8V387_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">N-terminal kinase-like protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">SCY1-like protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Regulates COPI-mediated retrograde protein traffic at the interface between the Golgi apparatus and the endoplasmic reticulum. Involved in the maintenance of the Golgi apparatus morphology.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8V387"/>
    <dbReference type="EnsemblMetazoa" id="ACON001091-RA">
        <property type="protein sequence ID" value="ACON001091-PA"/>
        <property type="gene ID" value="ACON001091"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_013481"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001091"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034147"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051177">
        <property type="entry name" value="CIK-Related_Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12984:SF3">
        <property type="entry name" value="N-TERMINAL KINASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12984">
        <property type="entry name" value="SCY1-RELATED S/T PROTEIN KINASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="18"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="584"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="746"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="609"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="654"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="688"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="756"/>
            <end position="806"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00040972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00042347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00056114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001091-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="889" mass="98142" checksum="A7E5501A1FC56C51" modified="2020-08-12" version="1">MWSFFSRDSSKDFPYEIGDPVQQFNTKSIWSLFRGKRKGSNDEVSVFTYDIKNGSDIKLELARAALKRLKTLRHPSILQFLDSLESDKVLYVATEPVEPLGTHIGNLAAQGPQRDLYLAWGIFQITRALSFLNNDGNLRHNNVSIWSVFINTSGEWKLGGLEYVSSAELPVVPPIKIPPSLEIYDPPEKNDASKIKTATKCSSDMWGLGCLVWESFNEPLKTRGHLKNIERIPKSLAPLYCELVGATPANRPNPADVITKCRKPGGFFKNDLVDSLLFLEEIQIKDKIEKMRFFSSLTAQIDNFPDNVCRYKILPQLITAYEYGDAGSAVLAPMFKLGRLLDEGEYQKRIVPCVVKLFASTDRVTRSRLLQQLDLFISHLQPNVVNDQIFPQIAHGFLDTNPTIREQTVKSIIHLAPKLNYNNLNVEVLRHFARLQSRDDQGGIRTNTTVCLGKIAPHLHPQVRQRVLVSAFIRAMRDPFPPSRVAGILALAATQQYFLLNEVAIRILPALCPLTTDPEKSVRDPAFKTLRGFLGKLEKVSEDPSLRESMEADVHTATPSLGNAAATWAGWAVTAVTAKFYRSQSDTARPKPPLTGKTLSKPASLEQPSSSSISTTTSSVTSMTSLEHESADTSASVSDYGPDNWDQENWGDMDTSQDPSSPMTGTSNQPNSNLNMIGSVSDVRDGWDNEDWGSLEEDPNETELNEKDEDELNQVQPKDEFTTNNPTAILTDLVLSINNSHTDSSYNMGRAGGGSSSNNSTTNTISSSLTENTVKAVTTNSNSRSTCNSTNANNSNNNSINNQGNHINSPSKMIALKNMNNLINISSSNSNQLSDWNNIDDTGAGWGDGEFETLNESSAGMILRCNNAPKIPPNSYAPLSVKPKSLTFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8V9D9</accession>
    <name>A0A6E8V9D9_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Facilitated trehalose transporter Tret1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">High-capacity facilitative transporter for trehalose. Does not transport maltose, sucrose or lactose. Mediates the bidirectional transfer of trehalose. Responsible for the transport of trehalose synthesized in the fat body and the incorporation of trehalose into other tissues that require a carbon source, thereby regulating trehalose levels in the hemolymph.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Trehalose transporter subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040239428.1">
        <property type="nucleotide sequence ID" value="XM_040383494.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8V9D9"/>
    <dbReference type="EnsemblMetazoa" id="ACON001027-RC">
        <property type="protein sequence ID" value="ACON001027-PC"/>
        <property type="gene ID" value="ACON001027"/>
    </dbReference>
    <dbReference type="GeneID" id="120959815"/>
    <dbReference type="CTD" id="39458"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009687"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON001027"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040419"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051119">
        <property type="term" value="F:sugar transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015574">
        <property type="term" value="F:trehalose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17358">
        <property type="entry name" value="MFS_GLUT6_8_Class3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000055">
        <property type="entry name" value="Facilitated trehalose transporter Tret1-2 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044775">
        <property type="entry name" value="MFS_ERD6/Tret1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050549">
        <property type="entry name" value="MFS_Trehalose_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48021">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48021:SF1">
        <property type="entry name" value="GH07001P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="61"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="91"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="116"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="149"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="174"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="259"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="297"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="323"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="357"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="393"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="426"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="15">
        <location>
            <begin position="1"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="467"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="475"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00060205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00069106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON001027-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="488" mass="52473" checksum="13C77BF465C1E228" modified="2020-08-12" version="1">MSKFVVNNVAGESGRKLPQYIAGLAASGGALAAGTFLGWTAPTDKPLTVEGEYGFPVSDEQFSWVGSISNLGAALMCFPIGYMMKLIGRKWSMLAMVLPLVLGWLLIIFAENVAMLMVGRFFLGVGGGAFCVAAPTYTAEIAQPSIRGTLGTFFQLMVTVGILFVYAVGAGVDVQVLSIICGVIPLVFGAIFFFMPESPYYFVEKGRYSEAASSLKWLRGAQYDENAEIEDLKQADEKVKAEAIPMLVAFRQKATVRALAISLGLMFFQQLSGINAVIFYNSAIFASANGGKEMSSASIIVGGIQVVATLLASVVVDKVGRRILLLVSDLMMAVSTILLAVYFQLKQDDPAKVDDLSWLAVLAVCLFIAMFSIGYGPVPWLMVGELFANNVKAFASPVAGVFNWLLAFLVTKVFTNLKDAMGEAGVFWLFSGISLLGTVFVFLVVPETKGKSLNDIQRLLAGEKLNADEPAGLPEPRDERDERPAQVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VAT2</accession>
    <name>A0A6E8VAT2_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Kinase</fullName>
            <ecNumber evidence="6">2.7.-.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the inositol phosphokinase (IPK) family.</text>
    </comment>
    <dbReference type="EC" id="2.7.-.-" evidence="6"/>
    <dbReference type="RefSeq" id="XP_040222862.2">
        <property type="nucleotide sequence ID" value="XM_040366928.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VAT2"/>
    <dbReference type="EnsemblMetazoa" id="ACON002194-RB">
        <property type="protein sequence ID" value="ACON002194-PB"/>
        <property type="gene ID" value="ACON002194"/>
    </dbReference>
    <dbReference type="GeneID" id="120949551"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_011292"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON002194"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033160"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000828">
        <property type="term" value="F:inositol hexakisphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032958">
        <property type="term" value="P:inositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.160:FF:000001">
        <property type="entry name" value="Kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.160">
        <property type="entry name" value="Inositol polyphosphate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005522">
        <property type="entry name" value="IPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038286">
        <property type="entry name" value="IPK_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12400">
        <property type="entry name" value="INOSITOL POLYPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12400:SF97">
        <property type="entry name" value="KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03770">
        <property type="entry name" value="IPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 6" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="26"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON002194-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="485" mass="54474" checksum="9EA021DD4ECF635D" modified="2020-08-12" version="1">MSLFTSSNSRPRSLMEALLAKKIEAASQQTGPGGSRLIRTDSMDSTSSIGSHGSLILGDDVCRCDDCLLGIVDRYTIGPKETASGIKKPSGWRKLRNIVQWTPFFQTYKKQRYPWVQLAGHQGNFKAGPDQGTVLKKLCPKEEKCFKVLMKDVLRPYVPEYKGQVNSEDGESTYIQLQDLLSDFYQPCVMDCKIGVRTYLEEELSKAKEKPKLRKDMYEKMIQIDQNAPTEEEHRAKGVTKPRYMVWRETISSTSTLGFRIEGIKKSDGTSSKDFKTTKSRDQICEAFREFTDGFPHAMPKYIQRLKAIRATLEYSEFFKSHEVIGSSLLFVHDRHKASVWLIDFAKTVALPESVAITHDSKWKVGNHEDGYLIGINNLIDIFSEVHDRQTLQCLTERIPRLSLPDDTNGIDANSDDPLRHDGCNRNSEISSAAVPAVPDRCVDKIGCVNNNNNYDCKPEDQQLVGNSTADTSTGLRDCIGSSSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VHB1</accession>
    <name>A0A6E8VHB1_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Annexin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="domain">
        <text evidence="6">A pair of annexin repeats may form one binding site for calcium and phospholipid.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the annexin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VHB1"/>
    <dbReference type="EnsemblMetazoa" id="ACON003790-RD">
        <property type="protein sequence ID" value="ACON003790-PD"/>
        <property type="gene ID" value="ACON003790"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004607"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003790"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_029479"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012506">
        <property type="term" value="C:vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001786">
        <property type="term" value="F:phosphatidylserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032509">
        <property type="term" value="P:endosome transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000001">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000002">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000004">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000010">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="3 6" id="KW-0106">Calcium</keyword>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007831"/>
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        <source>
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        <source>
            <dbReference type="RuleBase" id="RU003540"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="324" mass="35792" checksum="9EC6214655270201" modified="2020-08-12" version="1">MSSSQYYPFQCTPTVYPADPFDANEDAATLRTAMKGFGTDEKAIIEVLARRGIVQRLEIAQAFKTSFGKDLISDLKSELGGKFEDVILALMTPLPQFYAKELHDAISGIGTDEEAIIEILCTLSNYGIRTIAEFYEQMYNVSLESDLKGDTSGAFKRLCVSLVQGNRDENNGVDEGAAAADAQALFEAGEGQWGTDESVFNQILVTRSYQQLRAVFDIYESLAGHSIEDAIKREFSGAIEEGFKAIVRCVRSKVQYFAKRLHNSMAGLGTNDKTLIRIIVSRSEIDLGDIKEAFQEMYGKSLESWIKDDIGSDLGNLLAELATY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VHB5</accession>
    <name>A0A6E8VHB5_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
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            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
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        <property type="gene ID" value="ACON003794"/>
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    <dbReference type="VEuPathDB" id="VectorBase:ACON003794"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_039436"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="NOPS_NONA_like"/>
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        <property type="entry name" value="RRM1_p54nrb_like"/>
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        <property type="entry name" value="paraspeckle component 1 isoform X1"/>
        <property type="match status" value="1"/>
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        </location>
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            <begin position="11"/>
            <end position="22"/>
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            <end position="32"/>
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    </feature>
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            <begin position="537"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003794-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="568" mass="62776" checksum="7999B7BF48A2488D" modified="2020-08-12" version="1">MDVAVKQEINGSPLPQRPQQQPHGGGGGGPGPMQGNNQAGPDQHGPGGPGGPGGPGGPGGPGGPGGPGNKNMNRNNRNNSKNRQSMPNRNRGPGGNAGGGGGNPNNNGGPGNNRPGNNGGNPQQMGGEDFAYFINEKLRQLAGPLFEIPPIEAQDVKFSGRNRLYVGNLTNDVTEEELVDMFKVYGEISEVFMNKEKNYAFVRVDYLSNAEAAKRGLDGTTRKNRVLRVRFAPNATAIRVKNLTPYVTNELLYKAFEVFGPLERAVVQVDERGKPTGEGIVEFKNKPGAMAAIRYCTEKCYFLTASLRPVIVEPYTYQDDNDGLPEKSLNKKQNDFLKARQQGPRFAEHGSFEFEYGQRWKHMHELFKQKAESLKREMIMEEEKLEAQMEYARYEQETELLREQLRQREMDRDRQKAEWEMKERQVQEARQRNDIQIKTELEEMTTRIKRSDEELHRRQKENNMFMQQQMNDMGGMGSGGDGGGGSGIDNRRNFDMMNQVVGGGGGGGGGGGGNGNFGNMFEKEYLSELLRLLGTGSRDNAQGPNNINMKGKTANKKKTFRGGPRPNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VHB8</accession>
    <name>A0A6E8VHB8_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">poly(ADP-ribose) glycohydrolase</fullName>
            <ecNumber evidence="2">3.2.1.143</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the poly(ADP-ribose) glycohydrolase family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.143" evidence="2"/>
    <dbReference type="RefSeq" id="XP_049461488.1">
        <property type="nucleotide sequence ID" value="XM_049605531.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VHB8"/>
    <dbReference type="EnsemblMetazoa" id="ACON003831-RA">
        <property type="protein sequence ID" value="ACON003831-PA"/>
        <property type="gene ID" value="ACON003831"/>
    </dbReference>
    <dbReference type="GeneID" id="120950617"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007246"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON003831"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034243"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004649">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990966">
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    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:nucleotide-sugar metabolic process"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:regulation of DNA repair"/>
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        <property type="entry name" value="PARG_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007724">
        <property type="entry name" value="Poly_GlycHdrlase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12837">
        <property type="entry name" value="POLY ADP-RIBOSE GLYCOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12837:SF14">
        <property type="entry name" value="POLY(ADP-RIBOSE) GLYCOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05028">
        <property type="entry name" value="PARG_cat_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20811">
        <property type="entry name" value="PARG_cat_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PARG helical" evidence="6">
        <location>
            <begin position="70"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="PARG catalytic Macro" evidence="5">
        <location>
            <begin position="202"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="PARG catalytic Macro" evidence="5">
        <location>
            <begin position="674"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="354"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="380"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="484"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="607"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="701"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="412"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="484"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="607"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="701"/>
            <end position="713"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF20811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON003831-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="870" mass="97067" checksum="D0CCD0126C1FC2AD" modified="2023-06-28" version="2">MALVMLPCDLPWWSNVQKKLAQIEESSCLDVVIDVMQKLHELCNVSLDPDEDGKDTSVFDGLRHFVERTMDASERDHFLGHTIKALARHARNLKQYRPPRGLSFSLQQQADSYELSYRLVASLLANAFFSTFPKRTEKTHPTLQDFNFTHFFRGLVDNAVQRAKFRSFLYYFDWLERSGTALDGSLRVSRKVMTGKQWLTIEDWLECELPLCDVEIRHEGKLDKADPEMLQTVFASARLGGDVLADGDTQESIQFCTFPELLAVLLYVESLEDNESLQIENVHHVARIVDPRQKGPLLERIAEPKATSLCCIDAENYRALPSQQFEEDDILRELNKCLLAFRQNTSCPVALGEPPTVPAAKAGDKRTGAGLKLDQLETVSSKGRLSPIGERDREGSTPGSPDVYTTIFIRQATPNSKRSSSDSNKLEKDINKRRSWLSPERPNAPSVCSGTGSNRRGKFIILGSSGECLPVNRHPLRIPDSYQQQQGLTRPDTLSSCGSSQDEFHSAKDSFDEDDEDGLRAHAYTSELDTPERRYEFAQKLRAALEHDRTNGLSGSTDDSSYAVGISVAGSQIRDQDIRVRRGGSCGFVLDGSMDSNYDELEWNGQSQEKTAGGPQQNGTLERKGTNESSKYSFDSDLGSELEEVYEKFAKWLEEPISTDKPKKLDARDEAVLKFANSLLKRTLSESFVGIPFTEECLTSGNASGTNSTGSHNAAGSGGTGSLGQKEKILLHVRSLSLELAKHKHKLAAQLISQLAQKRTLNVNLRPVATGNWGCGASKRGDVQLKMVIQWMAASVAGLPYLSYYTAGNEKLSKLDTICRVLKDRKWTVGELAAATLSHARDILEDPFYYNNDRNYCMFFEKLIGLEKVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VJF0</accession>
    <name>A0A6E8VJF0_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RBR-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="2">2.3.2.31</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.31"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.3.2.31" evidence="2"/>
    <dbReference type="RefSeq" id="XP_040230446.2">
        <property type="nucleotide sequence ID" value="XM_040374512.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VJF0"/>
    <dbReference type="EnsemblMetazoa" id="ACON004573-RA">
        <property type="protein sequence ID" value="ACON004573-PA"/>
        <property type="gene ID" value="ACON004573"/>
    </dbReference>
    <dbReference type="GeneID" id="120954009"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007356"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004573"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_032135"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20338">
        <property type="entry name" value="BRcat_RBR_RNF19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20355">
        <property type="entry name" value="Rcat_RBR_RNF19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1750:FF:000001">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000856">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031127">
        <property type="entry name" value="E3_UB_ligase_RBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11685">
        <property type="entry name" value="RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01485">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22191">
        <property type="entry name" value="IBR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00647">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="355"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="408"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="13">
        <location>
            <begin position="135"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="12">
        <location>
            <begin position="139"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="26"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="616"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="687"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="800"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="889"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="44"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="78"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="113"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="630"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="695"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="716"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="826"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="843"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="893"/>
            <end position="926"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004573-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="926" mass="97317" checksum="EBE1FA8E81767914" modified="2023-06-28" version="2">MRGTSRNTFNLNFPLRRLLLQSARAARGSPPLGQRLCDAEKGGSNSGGSGGHGSGSNGGGKHDKTGSTGHSSNKHNSDASSQHSANSPGNLERGTGNSKLTPSSHKSSHSLHSKGGSSTTTGGAGTNASANAGKVPDECPLCYDVLPSSDEYYALLNCKHYACRACLENYLMIQISESRTDISCPQCSDSMHPTDIQTLLKAFPTAITKYEDFMVRRVLLSDPDSRWCPGPDCTYAVIATGCASCPRICCQRPGCDVQFCYHCKAEWHPDQTCDAARASRQSPTRALSEDEIKPCPRCQVLIVKMDDGSCNHMVCAICGSEFCWLCMKEISDLHYLSPSGCTFWGKKPWSRKKKLLWQLGTLVGAPLGIALVAGIAVPAMIIGIPVWVGRKIHTRYKSAGKHKRNLAVLGGVTASVIVSPVLAGLAVAIGVPILLFYVYGVVPVSLCRAGCGEGTKFEFDEEEDNGSRNHDAASVDAVSRIGATSIGEVSLSVASGSHLGVSSQHSAYGVANPSTTALAGSITGHRLEVQADVSTSETASAVTCVSEKSGNTLNDTASTKALAGSILSYKQQAESSAFSEDGASERVRFDNTVSFVIDSKKDSCDFLYSIPSDQDTAADKASLGSGRSFRSSERSFRDDFDSTSVSSAHKRINFGKLIPKLNSSQHRNLSVDSATGSYNIPENVSLEQELEQEETTAAQQHTASSNSSAATVALSRNATQTQMSHSSGLSAHQQQNTQSGSLNPSPEFTVRHTSFVANPSSKLAIASSSNSSSSSSSSCSSNEAINRAMLIEKPALSTYSDSVCSSGRDAEAAQNQRNESKPLDSPPLSASSLSDSSSHEHPAVAAASAPPKSRTHILRNLFFSLPNSTESNAPTEATVECGQVTVTGTRAKTASAPLTSPLSPSSPSTSSAPSTTLTGPSASTQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VJF5</accession>
    <name>A0A6E8VJF5_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glutamate dehydrogenase [NAD(P)(+)]</fullName>
            <ecNumber evidence="3">1.4.1.3</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">Ngousso</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-glutamate + NAD(+) + H2O = 2-oxoglutarate + NH4(+) + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15133"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.4.1.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-glutamate + NADP(+) + H2O = 2-oxoglutarate + NH4(+) + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11612"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.4.1.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Glu/Leu/Phe/Val dehydrogenases family.</text>
    </comment>
    <dbReference type="EC" id="1.4.1.3" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A6E8VJF5"/>
    <dbReference type="EnsemblMetazoa" id="ACON004362-RA">
        <property type="protein sequence ID" value="ACON004362-PA"/>
        <property type="gene ID" value="ACON004362"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004833"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON004362"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040704"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004352">
        <property type="term" value="F:glutamate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006538">
        <property type="term" value="P:L-glutamate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01076">
        <property type="entry name" value="NAD_bind_1_Glu_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10860:FF:000007">
        <property type="entry name" value="Glutamate dehydrogenase 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000100">
        <property type="entry name" value="Glutamate dehydrogenase 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006095">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006096">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006097">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033524">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033922">
        <property type="entry name" value="NAD_bind_Glu_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11606">
        <property type="entry name" value="GLUTAMATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11606:SF13">
        <property type="entry name" value="GLUTAMATE DEHYDROGENASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00208">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02812">
        <property type="entry name" value="ELFV_dehydrog_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00082">
        <property type="entry name" value="GLFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00839">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00074">
        <property type="entry name" value="GLFV_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamate/phenylalanine/leucine/valine/L-tryptophan dehydrogenase C-terminal" evidence="10">
        <location>
            <begin position="261"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="486"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="486"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="520"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON004362-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="536" mass="59665" checksum="81C0BBEE03354FC0" modified="2020-08-12" version="1">MYHLKTIAKNATIKQEVATLVKAVAAVQQPMAMNQQQPKRTYAEHQIPERLKDVSTAANPRFFDMVEYFFHRACQICEDKLVEDMKGRASMDEKKKKVKGILMLMQPCDHIIEIAFPLRRDSGDYEMITGYRAQHCTHRTPTKGGIRFSLDVSRDEVKALSALMTFKCACVDVPFGGAKAGVKIDPRKYSEHELEKITRRFALELSKKGFIGPGIDVPAPDMGTGEREMSWIADTYAKTIGHLDINAHACVTGKPINQGGIHGRVSATGRGVFHGLDNFIKEANYMAQIGTTPGWGGKTFIVQGFGNVGLHSCRYLTRAGATCIGVIEHDGSIFNPQGIDPKALEDYKNEKGTIVGFPGAQPYEGENLMYEQCDIFIPAAVEKVITAENAGKINAKIIAEAANGPTTPAADKILIDRNILVIPDLYINAGGVTVSFFEWLKNLNHVSYGRLTFKYERESNYHLLASIQQSLERFVFSDEESKNRFSDHLRHGLARSEARSRSKRPKPSRSASPVPRRRTSCTPGWTTRWSVRRAPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VLJ3</accession>
    <name>A0A6E8VLJ3_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="13">BTB domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Putative transcription factor required for axon growth and guidance in the central and peripheral nervous systems. Repels CNS axons away from the midline by promoting the expression of the midline repellent sli and its receptor robo.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VLJ3"/>
    <dbReference type="EnsemblMetazoa" id="ACON005245-RF">
        <property type="protein sequence ID" value="ACON005245-PF"/>
        <property type="gene ID" value="ACON005245"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009842"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005245"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_029613"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016199">
        <property type="term" value="P:axon midline choice point recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048813">
        <property type="term" value="P:dendrite morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008406">
        <property type="term" value="P:gonad development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035167">
        <property type="term" value="P:larval lymph gland hemopoiesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007526">
        <property type="term" value="P:larval somatic muscle development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045476">
        <property type="term" value="P:nurse cell apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007464">
        <property type="term" value="P:R3/R4 cell fate commitment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045467">
        <property type="term" value="P:R7 cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18315">
        <property type="entry name" value="BTB_POZ_BAB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000091">
        <property type="entry name" value="Lola, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051095">
        <property type="entry name" value="Dros_DevTransReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110">
        <property type="entry name" value="BTB DOMAIN TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110:SF111">
        <property type="entry name" value="LONGITUDINALS LACKING PROTEIN, ISOFORMS F_I_K_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3" id="KW-0221">Differentiation</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="11">
        <location>
            <begin position="32"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="509"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="538"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="115"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="301"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="161"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="175"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="212"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="322"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005245-PF"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="696" mass="73129" checksum="A44B7516CAB434A0" modified="2020-08-12" version="1">MDDDQQFCLRWNNHQSTLISVFDTLLENGTLVDCTLAAEGKLLKAHKVVLSACSPYFATILSQQYDKHPIFILKDVKFQELRAMMDYMYRGEVNISQDQLAALLKAAESLQIKGLSDNRSSSSSGTAAAGGGVHQKQSDAPAAKTLQPPVPASKASGLTIENKRSLKSELLESDVSGSREGSTSPTSRKRKKVRRRSVDTNNLLIDNHDQHSNSSSHSMHTAGGANVAAAVAAAGAPAANVASSLSSASSTAAAGSNTSANVLSVAAVAAAAVLKKTETVQQAAAETLKLQKQQAAAAAAAAAAQSSDDEERCGPGSVATGTEHDDGNDLSDNEHDGDGGSSVKSGSRHHHLRDGSRRGKTSTSSMGEMMIEPKSEYEDVQDENVEDLTMDDEDLMDELDQAGPSHGGDVSKTPANAAMAAAVAAAFASVANNSSYAATATGGGGGDVSFPEDSATLLHPQANQPWLVWKYLENKYSHTSYGSGGGSGGRGGSGSSVGEMAATTEAAFYKCRQCGKLYRTKYTWKRHERKECGVTPQFHCVHCDFATKYKHNLKTHNRIKHGEEECPPPSLTPLPCDAAPAVSVTNAVDSSIDPTASVSSVGSNDARMLVGGNTSAPIRIITTDDDNNIDGHDVDATDGVADDIAEDGNRANSFQTTASYKNNSNGYCEEMLEITNSTENNSALLFAISKNQSPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VLJ6</accession>
    <name>A0A6E8VLJ6_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SOWAHA-C winged helix-turn-helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the SOWAH family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VLJ6"/>
    <dbReference type="EnsemblMetazoa" id="ACON005008-RB">
        <property type="protein sequence ID" value="ACON005008-PB"/>
        <property type="gene ID" value="ACON005008"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004669"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005008"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_040155"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_042074"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058889">
        <property type="entry name" value="WHD_SOWAHA-C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14491">
        <property type="entry name" value="SOSONDOWAH, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14491:SF7">
        <property type="entry name" value="SOSONDOWAH, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25877">
        <property type="entry name" value="WHD_SOWAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="SOWAHA-C winged helix-turn-helix" evidence="5">
        <location>
            <begin position="7"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="80"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="172"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="452"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="86"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="111"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="215"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="252"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="488"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="498"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="521"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="552"/>
            <end position="562"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF25877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005008-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="562" mass="62239" checksum="64220A0990AA3AC2" modified="2020-08-12" version="1">MDTEPKELSMEEIRKFMVLNNYKLTNHTLVKHFRSFLTNKDTQDEARRRFKEYVNKIASVKHEGNEKYLVLRRKYYNGIPPEDAGTESQPMSPASRSYGLLGSDSEASPLRQPPPYRPPPVVSPDTVSYQGSPRGSVALNRRNSSDYVRSGSEISFGSEFASVDSRKVNPLLVRSNSNDTRSLPDGGRKPSVDMMVPYDDGAPPAIPPRKRVSESGGSIASNSRQNSVEERSTGLVSDTRSENKENVASAESEEKGVVASEENKLSVKEKMMKFNRFASEEEAKIPSPIGKKKPDKNAEETLNTENLMQHPNAKEWLIAAAKSDYQLLAKLSTEHPNLVKLQTALHWAAKHGNEDVVKLVAGKLKADVNARTGYTALHIATQFGRNDIFELLCNVYKADRDLLDWSGKKALEYQKQMTSVSESTFSSEYERKVSKHEKFFTLPSKTLSPLARGGTFMRRKRRQRPATSDQMTLRNFVDSFPHPPAGPRAPAAAGSAADTNNNNRPGSISSSNTAKTKPTISSAANTASATANPPPAEGASLERRNRSNQSFFRKKRNATNAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VLJ9</accession>
    <name>A0A6E8VLJ9_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="11">BTB domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Putative transcription factor required for axon growth and guidance in the central and peripheral nervous systems. Repels CNS axons away from the midline by promoting the expression of the midline repellent sli and its receptor robo.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VLJ9"/>
    <dbReference type="EnsemblMetazoa" id="ACON005245-RK">
        <property type="protein sequence ID" value="ACON005245-PK"/>
        <property type="gene ID" value="ACON005245"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009842"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON005245"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_029613"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016199">
        <property type="term" value="P:axon midline choice point recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048813">
        <property type="term" value="P:dendrite morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008406">
        <property type="term" value="P:gonad development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035167">
        <property type="term" value="P:larval lymph gland hemopoiesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007526">
        <property type="term" value="P:larval somatic muscle development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045476">
        <property type="term" value="P:nurse cell apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007464">
        <property type="term" value="P:R3/R4 cell fate commitment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045467">
        <property type="term" value="P:R7 cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18315">
        <property type="entry name" value="BTB_POZ_BAB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000091">
        <property type="entry name" value="Lola, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051095">
        <property type="entry name" value="Dros_DevTransReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110">
        <property type="entry name" value="BTB DOMAIN TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110:SF111">
        <property type="entry name" value="LONGITUDINALS LACKING PROTEIN, ISOFORMS F_I_K_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3" id="KW-0221">Differentiation</keyword>
    <keyword evidence="4" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BTB" evidence="10">
        <location>
            <begin position="32"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="115"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="301"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="161"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="175"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="212"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="322"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON005245-PK"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="502" mass="53607" checksum="733E471F8A165231" modified="2020-08-12" version="1">MDDDQQFCLRWNNHQSTLISVFDTLLENGTLVDCTLAAEGKLLKAHKVVLSACSPYFATILSQQYDKHPIFILKDVKFQELRAMMDYMYRGEVNISQDQLAALLKAAESLQIKGLSDNRSSSSSGTAAAGGGVHQKQSDAPAAKTLQPPVPASKASGLTIENKRSLKSELLESDVSGSREGSTSPTSRKRKKVRRRSVDTNNLLIDNHDQHSNSSSHSMHTAGGANVAAAVAAAGAPAANVASSLSSASSTAAAGSNTSANVLSVAAVAAAAVLKKTETVQQAAAETLKLQKQQAAAAAAAAAAQSSDDEERCGPGSVATGTEHDDGNDLSDNEHDGDGGSSVKSGSRHHHLRDGSRRGKTSTSSMGEMMIEPKSEYEDVQDENVEDLTMDDEDLMDELDQAGPSHGGDVSNCIDMLEPKVVIMDDGLTRYVCPQCGVKYKKLSALRGHMKECGKGAQCPLCPKIVTQRRNLAKHMERHRRDGLLEFHHFIDNFPSNINLII</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VPR0</accession>
    <name>A0A6E8VPR0_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Neurabin-1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Neurabin-I</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Neural tissue-specific F-actin-binding protein I</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Protein phosphatase 1 regulatory subunit 9A</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
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        <source>
            <strain>Mali-NIH</strain>
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        <scope>IDENTIFICATION</scope>
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    <sequence length="2014" mass="218326" checksum="A319306087E1154C" modified="2020-08-12" version="1">MDKSTATAAHPSAATAAAGPKVSQIANLFQRKPVELAQDVQTRSGDRPAADSATAATHSPTATVVRTESHAARFNNARALFEKLGEGRVNRPPPFSIKMSHSSSREDNLSDIGSGPDRSPSPKRKQLQQPPPPTAISNGIGKLDSNRILNQSRLKSEKPEKPEKPERKFNSRELIEKQKNWTSHFSKTRTTKGSDFNRCDIIRTVPGTGIIAGAGANAATAAATPPVNTAPTTNGNYSAIPNGSAKEPLPPPPFAAERNGHGVHHRLPSPSEPPPSPPKRQTPPPPPPEKGPRTANLRQNSFPSPTKTPPAVPPHATDPSSLSPTKLSPEKLKPPPRQAEPPPAVTAPHANLPPVAPVRSSTTVLTTVHHQQQQQQQLVEQQVPPPPPEKAIRKKSLEGNLDERLPAVPAPPTGNGGFKYPELGPLARRQSSEKDTGSGGLTSPAHAISSSPSPGASASSGPSSPIHTEDEKQENESTEKLMKAGSSIERDDSSRAEDSPPAHEAKPVEELNGKSSRTDDEHSGEQQQQPPQQQGGVTRRTKVSSICFDVPAAGLGNRPPSIISSSTTTDEGGFNEPSPEIKAKLKPAYEFDLPLPASPPRKVPIGEDRSPVGSPAEAKLTYADLGYRVRPDGTECDEIYGEVDVYRSAKQPAVGVPLARSQPSGAAASAGVPNGGQAAAQLAEPQVVAGPATLANGRSGPTERVVYASILPSSLGDNGSTTTMATDSELAFMDGERDEAKILDFDELSEMGEKGYHSAQILLDPTRTTLSQPADDDYSTKTLPEPPSLASLRPPPIPEGPPLDLQDTLKFADASDNEQDSLLPDEMTADEAERLLSSRQRPLLSDEQAREVEQILLNNQAKAEAEEPPAVRVVAGPRQQVPSVPEPAAKPQQLPPKQAPPTTTTAAVSSTAPTADEPDWLKDVLEAPKNATSSSSTAGSSMRDKPPPPPPPTSASRNNSTTSHPEDTSIGNNTTCDLLNQTILLDSSSMLADSYVDTGDSIPSVTTTTSDSINLSKLEESAAETTGALEDEGEATQEEEGQEEEQQQQQQQQQLASGRCVAAHRQQQQPQALAEGSVSEADVSADDESNNSAAGFYIPEYPPVRCKEVYVNPDGVHFFEDGNFWMEVPGLIEAERELDDDDLRGYVKKTTKVKFSCNPMQVFSTFSVNDYDRRNEDVDPVAASAEYELEKRVEKMDVFPVELMKGPEGLGLSIIGMGVGADAGLEKLGIFVKTITDNGAAARDGRIQVNDQIIEVDGKSLVGVTQAYAASVLRNTSGLVRFQIGRERDPENSEVAQLIRQSLQADREKEERVKRQLEDYIRRNAEISEDSTLPVSANSSVSEGPVSPTTNAAESLFETEAARSSDVESLRRILQELIKYQQTCNEAELLNERVKQTERELANIKKEANNYQNMLQQSQAQYLVLEKKYSRVKKILRDYQHRERDMIQFQEYYLQHLQEKDTEYNALVKKLKDRVISLEQELQETQRKAGFPVILPYDSASLKLTPQMSRRQPPKPVFQKLETDLSDTEFSDLSPDGEGEDGKTATVERKVPATTKDDDLDVAVPQHELLDNSLNKGKSDLVARGGLAKRNLPGKRSHSNSGSDCALDESDDDEGQLTRNATGTEGSHEYSGLYSTPQIVNRKVATLKGEDSGLPVYAQVNKERSSGGGHADHHQQQQQQQQHTTIPNIYRAAVGSDNGKLNSSYGSDLNASSYDSDLGSSTDKLKDSGLSSDSWMYPSRRPKGLKGITPPLLAEQLKERLAEREGRRGSGGGLIDDGSSRDSSDDYSEINQQQRHISPAASLSQNLLVEIKKAVNEAQPKVKNILPQNLSPPGGNGPWQQQQQQQQQQGAQSTSGGTTAGSSQGPPSPSSVSSGSTSPGAYSPSRTLDLSGSTSSFSSDRIRDTHQWKNGPVEEWSNEQVCQWLLGIGLDHHIPAFMQHSVEGGALLQLDKPDFKILNVGGDDKKLLKRNIKELRRLNEKERKQNEKERREREKLFKKQEKKAEKEQKKRYQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VPR5</accession>
    <name>A0A6E8VPR5_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="10">BZIP domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bZIP family. PAR subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VPR5"/>
    <dbReference type="EnsemblMetazoa" id="ACON006376-RA">
        <property type="protein sequence ID" value="ACON006376-PA"/>
        <property type="gene ID" value="ACON006376"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_007649"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON006376"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034902"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14695">
        <property type="entry name" value="bZIP_HLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000007">
        <property type="entry name" value="hepatic leukemia factor isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040223">
        <property type="entry name" value="PAR_bZIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11988:SF27">
        <property type="entry name" value="GH27708P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11988">
        <property type="entry name" value="THYROTROPH EMBRYONIC FACTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07716">
        <property type="entry name" value="bZIP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="9">
        <location>
            <begin position="399"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="92"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="329"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="431"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="35"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="103"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="153"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="170"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="338"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="355"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON006376-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="465" mass="51453" checksum="2C5E47274373C825" modified="2020-08-12" version="1">MNMEFQPLPPVSTMNIGPPTPSPSPHIQILSNGPNGSGPTGAGGGGGPGGGHNGMGNQGVHSQLLGPMPPPSILPIGMPLQQVVHNLQHLTNVGGLGMGGNPQQQQQQQQQQQQQQQQQQQHQHQQQQQQTHQNLLTSNTPGANGLTGIHHGGTQNLPPTSQLLQAIHQNTSNNNNNGANNNNNNNNTINLNNNSNGNNNNNNSITQYQVQQLWRHHAYLNGNVLLSYPSGTRNNKDICGEDKHKEENDPWNVETHSAFLGPNLWDKTLPYDSDLKVHQYADLDEFLSENGIPYDGIPNSNLGNSTSLTGQRSDSIGHCAGLSLSGLGQVTTKRERSPSPSDCMSPETMNPPSPADSTFSMSSTRDFDPRTRAFSDEELKPQPMIKKSRKQFVPDEMKDDKYWARRRKNNMAAKRSRDARRMKENQIALRAGYLEKENMNLHREVEQLKQENMELRARLSKYQEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VRV0</accession>
    <name>A0A6E8VRV0_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VRV0"/>
    <dbReference type="EnsemblMetazoa" id="ACON007064-RB">
        <property type="protein sequence ID" value="ACON007064-PB"/>
        <property type="gene ID" value="ACON007064"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012428"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007064"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034009"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="84"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="21"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007064-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="131" mass="14595" checksum="7F830F9230ECB6CE" modified="2020-08-12" version="1">MGDVAEELFNAMGGPEGGAGAAAESAPGAENPFNPEDFKMPTMEDFLSMLEGMDNLSDEEKEELKAGVLQNAMRRKMQAGLSDYLVFLVMLAIMLAVFAFFGYKLYLSLTEKERKREEKLKAKQEKSKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VS79</accession>
    <name>A0A6E8VS79_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the peptidase S1 family. CLIP subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VS79"/>
    <dbReference type="EnsemblMetazoa" id="ACON007043-RA">
        <property type="protein sequence ID" value="ACON007043-PA"/>
        <property type="gene ID" value="ACON007043"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012577"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007043"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_031074"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000028">
        <property type="entry name" value="Serine protease easter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000068">
        <property type="entry name" value="transmembrane protease serine 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051333">
        <property type="entry name" value="CLIP_Serine_Protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24260:SF136">
        <property type="entry name" value="GH08193P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="3" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Peptidase S1" evidence="9">
        <location>
            <begin position="4"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="9">
        <location>
            <begin position="271"/>
            <end position="532"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007043-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="532" mass="59918" checksum="3BDEEF1BB5E2F830" modified="2020-08-12" version="1">MGLVTKGIIAEPGDWPWHVALFAHMKSEKPAYKCGGSIISQHFVLSAAHCIKEPNPDHYFLKAGIHHLNNDNDTSVVVYNLFEIILHPKYDRHTFYNDIALMRPDRAISFASFSIFPICLWPTHNATLIDVLSRSGIAVGFGFDETHRISETLQQASMKVIEKQQCIEQLPEHVRFLPQDAGKMCAIGTESGANVCSGDSGGGLYFAKDQVWYLRGIVSAAARRDLDTGEATCNAALPATYTDVAQYTTWINAHQQLVDQRNLLKLEDCGVARNSEVQDEMKKPVFNQYPWNALLEFRQLDKVQTHLVCSGILIHPRYVLAVGHCVEGVFSNYKLQSVRLGEYNIRSVEDADPNAPATTITSQSVDIEQVFFHPNYNKPQYANNLALLKLKHNADTSKPNIKPICLPAVDDYKETFLTVSGWKRNKLIFPKMERDAMNLTSSITCRSEYEKLGVVLPPTEDVLCAVYKTRPKGHCHNYATGSPLQYIKRVDKVPRYFLAGLMVFSFPHCRAEGSEMFVNLERACDWIKDTVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VTZ1</accession>
    <name>A0A6E8VTZ1_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="3">SBF1/SBF2 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VTZ1"/>
    <dbReference type="EnsemblMetazoa" id="ACON007789-RA">
        <property type="protein sequence ID" value="ACON007789-PA"/>
        <property type="gene ID" value="ACON007789"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_012299"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007789"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_037840"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039872">
        <property type="entry name" value="KIAA0513"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022096">
        <property type="entry name" value="SBF1/SBF2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13663">
        <property type="entry name" value="SIMILAR TO RIKEN CDNA 6430548M08"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13663:SF2">
        <property type="entry name" value="SIMILAR TO RIKEN CDNA 6430548M08"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12335">
        <property type="entry name" value="SBF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SBF1/SBF2" evidence="2">
        <location>
            <begin position="279"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="385"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="94"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="400"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="421"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007789-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="526" mass="58913" checksum="4BA87B07F15DCF28" modified="2020-08-12" version="1">IDAALSLTSSSDTETTANRNQMKVDDMLRRKYESSNMKHPDDTRGEHQIDYELRRAKERRAHEEVLSGRYRNPAFSHLLPPPTPVPSTPPQPVPTATSTTKPMTTTTTTTTSAGKESEERVTERAPSHSLSADFSHSNFSQNSCSLDSKSPQPSAGGPDLGETVGRLGATNNAVATLDGFPWGRDFYHGREPRLGIPSSASTSDNESCAFSGTLESSELKMWIRSESENSVPSWASSISLDSQSEEAVLDFMKRFVRILFHDSSSITLQLKSDFGQFARTETGRLWFSRLVNAQRAKTKRVDESTFYSLIQYFAIVLFECAESEDYSPAKTLMNMCFTFYHDIDVPGCDPYREYLYTYLRDQPIWQTLRFWNAAFFDALQTERKHRPVPPTVKHSPSAPPSHTKQSSKGSGTVPNIGDPFLGGTEEAATSAATLTVPEQERSSSTSACDRTDPAELQEDKHYHQNLSFGQLGSFTCNMHAFGLSRELCNEFLVKQSVIANLSPEQQKLLQENVDRMYRETDPWREN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VTZ5</accession>
    <name>A0A6E8VTZ5_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Synaptotagmin</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">Ngousso</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Secretory vesicle</location>
            <location evidence="2">Synaptic vesicle membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the synaptotagmin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040233092.1">
        <property type="nucleotide sequence ID" value="XM_040377158.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VTZ5"/>
    <dbReference type="EnsemblMetazoa" id="ACON007942-RB">
        <property type="protein sequence ID" value="ACON007942-PB"/>
        <property type="gene ID" value="ACON007942"/>
    </dbReference>
    <dbReference type="GeneID" id="120955921"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_008180"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007942"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_036380"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031045">
        <property type="term" value="C:dense core granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001786">
        <property type="term" value="F:phosphatidylserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048791">
        <property type="term" value="P:calcium ion-regulated exocytosis of neurotransmitter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007626">
        <property type="term" value="P:locomotory behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048488">
        <property type="term" value="P:synaptic vesicle endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08385">
        <property type="entry name" value="C2A_Synaptotagmin-1-5-6-9-10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08402">
        <property type="entry name" value="C2B_Synaptotagmin-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000007">
        <property type="entry name" value="Synaptotagmin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000016">
        <property type="entry name" value="Synaptotagmin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001565">
        <property type="entry name" value="Synaptotagmin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10024">
        <property type="entry name" value="SYNAPTOTAGMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10024:SF227">
        <property type="entry name" value="SYNAPTOTAGMIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00360">
        <property type="entry name" value="C2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00399">
        <property type="entry name" value="SYNAPTOTAGMN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="11" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0770">Synapse</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="86"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="14">
        <location>
            <begin position="159"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="14">
        <location>
            <begin position="293"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="35"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="121"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="46"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="137"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007942-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="437" mass="49137" checksum="E6A6005EE9E1064D" modified="2020-08-12" version="1">MAPNSRLWKREAEVESDAAKLADVASTLATKIFGHKQKHHTTTTESELDAEENEDFSTATPTKGEAEKLGDKAAEMTEALAHEMGIPTWGLVSIIIVVVLIILGICGFCIRRCFRKRRSKDGKKGMKGVDLKSVQPDMEELTENAEEGDEGESKQSEQKLGKLQYKLEYDFNSNALTVTVIQAEELPALDMGGTSDPYVKVYLLPDKKKKFETKVHRKTLNPVFNESFTFKSLPYAEAMNKTLVFAIFDFDRFSKHDQIGEVKVPLCQIDLAQTIEEWRELQSVEGEGGQENKLGDICFSLRYVPTAGKLTVVILEAKNLKKMDVGGLSDPYVKIALMQNGKRLKKKKTSIKKCTLNPYYNESFSFEVPFEQIQKVNLVVTVVDYDRIGTSEPIGKVVLGYNASGTELRHWSDMLASPRRPIAQWHTLKDPDDDKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VTZ7</accession>
    <name>A0A6E8VTZ7_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Sodium/potassium-transporting ATPase subunit beta</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the X(+)/potassium ATPases subunit beta family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8VTZ7"/>
    <dbReference type="EnsemblMetazoa" id="ACON007790-RD">
        <property type="protein sequence ID" value="ACON007790-PD"/>
        <property type="gene ID" value="ACON007790"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009961"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON007790"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030366"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005890">
        <property type="term" value="C:sodium:potassium-exchanging ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030007">
        <property type="term" value="P:intracellular potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006883">
        <property type="term" value="P:intracellular sodium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036376">
        <property type="term" value="P:sodium ion export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1660">
        <property type="entry name" value="Na, k-atpase alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000402">
        <property type="entry name" value="Na/K_ATPase_sub_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038702">
        <property type="entry name" value="Na/K_ATPase_sub_beta_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11523">
        <property type="entry name" value="SODIUM/POTASSIUM-DEPENDENT ATPASE BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11523:SF46">
        <property type="entry name" value="SODIUM_POTASSIUM-TRANSPORTING ATPASE SUBUNIT BETA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00287">
        <property type="entry name" value="Na_K-ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON007790-PD"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="259" mass="29039" checksum="604D3FA1F82D905B" modified="2020-08-12" version="1">MPAEKRSSQPRIVRTYEFPTKPEKQTLGQYLYNSQDGKILGRTSKGINSIQYVAANKSDVAVWVNMLNDFLAPYADHTLLPGGGKNQVICDFNTPPTAGNVCAFDVKNLGPCSAAAGYSYNRSAPCIFIKLNRIYGWQPEFYEDVDDLPTDMPDDLASHIRSLPAPDRRQVWVTCKELTTTDENQLGPIEYLPSQGFPAYYYPYMNIDGYLSPLVAVHLARPKPKTTISIECRAWAKNIIYRGGSRDRAGSVQLFLRID</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8VYC1</accession>
    <name>A0A6E8VYC1_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Kinase</fullName>
            <ecNumber evidence="4">2.7.-.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">Ngousso</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the inositol phosphokinase (IPK) family.</text>
    </comment>
    <dbReference type="EC" id="2.7.-.-" evidence="4"/>
    <dbReference type="RefSeq" id="XP_040233898.2">
        <property type="nucleotide sequence ID" value="XM_040377964.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6E8VYC1"/>
    <dbReference type="EnsemblMetazoa" id="ACON009589-RB">
        <property type="protein sequence ID" value="ACON009589-PB"/>
        <property type="gene ID" value="ACON009589"/>
    </dbReference>
    <dbReference type="GeneID" id="120956477"/>
    <dbReference type="CTD" id="34300"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_005426"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON009589"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_030485"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000828">
        <property type="term" value="F:inositol hexakisphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032958">
        <property type="term" value="P:inositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.160">
        <property type="entry name" value="Inositol polyphosphate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005522">
        <property type="entry name" value="IPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038286">
        <property type="entry name" value="IPK_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12400">
        <property type="entry name" value="INOSITOL POLYPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12400:SF26">
        <property type="entry name" value="KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03770">
        <property type="entry name" value="IPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON009589-PB"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="344" mass="38801" checksum="AA3BDB5B4E9D4159" modified="2023-06-28" version="2">MNIEAERTATEYLVDQGVPTANANKSLLSFLAINALELSAPASPVLLECNAPTVPPGWLQLSGHPKSIAPLANGIVRKRISGPNDTELLAYRQLMVDPHAIKVVPKFLGVHQVAGEHFIDLHNMLHGFTEPNVMDIKMGFRTFTESEVSNTALREDLYQKMIAVDPSAPSAEEHARQAITKLRYMQFRENMSSTQEKGFRIEALRMRGCSPVTDLKTIKTDHQIRTTIGHFVNGRRSIAKDILKRLRQMRTLIEKSEFFQRHQVLGSSVFIVYDDHQVGVWLIDFAKALPLPPGTKVTHRRRWQMGNCEEGLLHGFDELIRTMEAVQQQAQHSSVRHQSRATDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W1R8</accession>
    <name>A0A6E8W1R8_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="4">RAVE complex protein Rav1 C-terminal domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_040231572.2">
        <property type="nucleotide sequence ID" value="XM_040375638.2"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON010490-RA">
        <property type="protein sequence ID" value="ACON010490-PA"/>
        <property type="gene ID" value="ACON010490"/>
    </dbReference>
    <dbReference type="GeneID" id="120955112"/>
    <dbReference type="CTD" id="31557"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_004805"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON010490"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035701"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043291">
        <property type="term" value="C:RAVE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000208">
        <property type="entry name" value="dmX-like protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001065">
        <property type="entry name" value="Dual oxidase maturation factor, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052208">
        <property type="entry name" value="DmX-like/RAVE_component"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022033">
        <property type="entry name" value="Rav1p_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13950:SF9">
        <property type="entry name" value="RABCONNECTIN-3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13950">
        <property type="entry name" value="RABCONNECTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12234">
        <property type="entry name" value="Rav1p_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="RAVE complex protein Rav1 C-terminal" evidence="3">
        <location>
            <begin position="1866"/>
            <end position="2171"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="3400"/>
            <end position="3441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="144"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="458"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="680"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1759"/>
            <end position="1783"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2286"/>
            <end position="2309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2336"/>
            <end position="2373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2869"/>
            <end position="2910"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="3073"/>
            <end position="3100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="680"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="698"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1761"/>
            <end position="1772"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="2286"/>
            <end position="2307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="2336"/>
            <end position="2347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="2884"/>
            <end position="2895"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="3073"/>
            <end position="3092"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010490-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="3495" mass="383555" checksum="C688E8CDD3BE60F9" modified="2020-08-12" version="1">MNCHQILSGACNAGDRCFAVGSVEGVPFTAYAAGCNIVVLASNFERVQIIPGAVHNYIRISALDASTDTGKIAAAYEDKVCIFEPTPLIHTERASTHGLEYKWVQTGSLQAASHISSLSWNLEGTRLLTGGTVLQLWHERLSKQDDDEPGSVKFEIGGERDPKTPTGEEEADSSSWDCVWKCHTATPVHHMAFSPDGTLFATSGKSDRLVKVWYENKYTLFPNKSFDQSQSQSFAGAPEISYGFVYVAHPRAVTHLSWRKTSKYMPKGSVSNMLVTSCLDNICRIWVETVLPEDGLVNMNQFDPLASQNPKFRTHRHKHRFMQRLKHMKTCFHIRRHAKHSTGGPNGLNGMGPGFGGPGASSGFGNAPIPTLPSTYSVHDFHSYGYHGTGMRSGLHFHLAASINAETDIPLVPSMHTTDPSTQPNFILHWLNNKEMHFTLQAEGILQEMTRKVIEKEEMQNASGSNSEGGGGVGANDPGGSLLMEDGSIADHHRKGQLRSALSQEESNSSEEHPPMHSQHRSHSIGAGSHVGLPSHHSLSNTTSINSLANDGNLHVTDSLDMKIDCLLRDWQHSPDLLFSIHPIDGSYLIWVVEWLDEYHPGSYRQAQVSFSTRIPSAFPLGDAMSMSTTVALYNTGGNLGFRDMIMRGPKSSCGAGGLSNGGQLDVVSDVVTSLPSVMEENEANEDDNAEQASNAETEDRGGEETGDHHASKKDHTGADGAPLEQADILTAPPSPTISMVTKHSNGTLNLWQLTFADRSKFSQVLSIGHASRASGHRFRVNDITCHPVLPLLLTTSHHNIPDIPTGEPAQPGGVTGNRSAGMHRSKDVCAPSGFCSELILWRVDAVGPLSQSGGVSELARINSPEISAFSNVAWIPTLLPSTTLGNLSNSPSACFVASDGECLRVYQAVIDARTLLAEISSSERRCRRMDSLMSLSTESSENAGMLPPILHDKIKIVSQQSTARPGCIIQLDAIDDATHDWQNTQFLHVFQEQLITGERTESMDPFAGANTTLPAGSSIDADGLNPDLKPTIGLMDGGMGAMVDLQRNAQFEQPFYLVVLERTQNGTTLHMWRIVIASQPAAADEMSSSMMYVPDSNLVQDLDDPENLHRPSVVPDDLPKSAGHHRAPLDHASHVNISTTKVCTQELPLPDGVDVIHAAPAAGHLSSSSIYPACFAPYIIVTACSDSTVRFWKCKSTTRKEGGKGAGESESSQVPHYEWCEWEMIRKDQESTIDVNGQLLHISAAYSGRIACAYKYGKSFTRPTKSSDPETRFVNLSVAIYECESTGGSEWVLEDTIHLKNIHLPRIQVDHNLDLSYLYDTRTLQKKQRLNQVLHTLSHDDARSPRSVNGDVTPEALAKPGTGLLAVPSFSTLQSLRKSITEHGNTCPITQKHLVQLDWVSKEDGSHILTVAVGSKILLFTPVSSDLAQSNMKAMKESQSTNRPLLRKASSLAQPHFNDEIRWMKLRQIELTTADGLPPLPMQISWVRDGIFVAGMDSEMHVYSQWKPQSNSLYNVNPQLDIDDMYDTRNLKDEDLRSMAQDSTQRRLANVSSMPVLSRVSSINLNQMLSSADTKKKKTLAAASTGQIGAVAAAAAAADQQQQPGHHDYMTDYGLFEASRIACPVLPQYHPKQLMELLNSGKIRWVKAILAHLVRCISGSYTVRGCGGDEESLNRQRGWSRTRTLSISCPAGTVSPLEPRGSTTQIPEELTLDYAEITSIPPLPLWTLLAADKESTSSNLQQNDEVKDYNELFESNTADESLDDLLDDESGGGETNRHMDRRSSLPEKHFLSHFGPRQGQLLSRLLTHTHLPGLSSLDQMHLLALADTVSTCNIDFAERFAIDAAKTAIAKENLTGVPNTENISTDSLDDCGLRFLLAMKHYNYLLRCLPISQRHSFQKQGVGTSNIVWAFHSESEEELLNLIPSYVKGQPKWHVLKELGVGWWLRNNNLLRQCIERLAKASFQANQDPLDAAIYYMAMKKKSVVWGLYRSKRDEKMTAFFANNFAEDRWRKAALKNAFALLGKQRFEHAVAFFLLANSLNDALEVCLTKLEDLQLALVITRLYEGEHDATPPSFKKLLYEEVLGCDKNGENQDLSRAHPDPFLRSMALWILKDYSGSLSTLLDSQIGSMHPLFDDESAALGSASHHDAGHSTNPNVFNFYVYLRTHPLLIRQHIASSAQDKKRAQVVIAGFSYGDTSTVSGGLATDKQIQLEDSITPLERQLYFTTAHAHFKAGCPALALEVLSKLPTKVIEQETSNVQSPASGLDAKDQSTLIATGTLDWSNETSGKETATSFDWGASTGNSTAMDWGAPVSTQLNGGEDEFKIVWDDDGAAAGDDCESSDDDGGIQIKKKPEPDSLPLNKNTDTEFEDGGSAGGGSLDIMAQQLKFIACLKILMEELSTLATGFEVDGGQLRYQLYVWLEREVEALKQLCNYSTGDSENTAIEDTTTNAEAMDRDTPVLANKFQHDKPTLHEILIQEKQDFEAKVQRAARRKRWLKANETLLRTLLSYCSLHGASGGGLASVRMELVLLLQELQQEKTQQQLLSPLPFPTTLPLLSACVAGNKTVIADPLRYLQSHTHDMLQTMVNMRTPPHMNRAYTFPSGIFVLRDLAVALSACIYQSLCDSDTFSVKQSSSSIDGGSQGCHSPGMETIAKLNASCQSTHLMANAAAHQRRRKYSTDEPLAVSTPPSKWPGVTNLRALLAREKDEDTPRLNVLLCESFVAAYMALFVYALTTCDCHILYRLAGQNFCDSSWSTLFGGGVKKLLRKATSHAQTAQTVQAQMQQESMEGSAEAGASSAEGSVWGAVTSLTKHRVKLNMKLLGPFAGPQGASNMKEDKPTYREQFVPPEMSMVSYFLTKPIPKGLGADEEEYDSADSAVSDLDDEDEDEDVFNDPLNNDPKASQVSAAVRKHRLENTEHSNPNSYSWCIMRFALIRVAQNQLQSFIAIAGIEMQELPVSSPLVHGILRSLSSWQDLLKEELENRGPASVDYIPGCFIESEAKGPAIHKYRSLLERSNTPFSPCLAASAPARRLWNYLVRIESVQEIFIRAVFGKKKSSSSAFDPGTGLGSASASVVDSGTPVGNENNGSLAHHQQHNQNIPEPVRIIHKDQEQISAFCLNMVNPGLLALATPREVQEMDISLLLESPNWMEDECEMDIMNLSKDIESLASSNFLVIHTSTDKHLLNQNISSAQNYGAPSSPQPGIAGQSGRGASVVLKHKVDNIKRMCAHPLMPLYLTGGQDGSVQMWEWGHQQVVCTPRSPGTFAKVTRCRFSQHGNKFGIADGDGNLSLWQVGLASQNNRPFFTYQCHNKGITDFVFLGSCSLVATAGHSSESKNVAIWDTLLPQKKALVAAFTCHDQGASSLAYAPQHQLLISAGKKGDVSIFDVRQRILRHRFQAHEHPIKCLAIDPNEEQFVTGSADGDIKVWGLSIHTQLYSFMGEHARSSFFKHIGQGVTQLQIDQGGRLFSCGADGSMKVRQLPDRESIVHSLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W236</accession>
    <name>A0A6E8W236_ANOCL</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Myosin heavy chain</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">Ngousso</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Muscle contraction.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Myofibril</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_040234090.1">
        <property type="nucleotide sequence ID" value="XM_040378156.2"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON010147-RK">
        <property type="protein sequence ID" value="ACON010147-PK"/>
        <property type="gene ID" value="ACON010147"/>
    </dbReference>
    <dbReference type="GeneID" id="120956570"/>
    <dbReference type="CTD" id="35007"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009622"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON010147"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_041963"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031672">
        <property type="term" value="C:A band"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032982">
        <property type="term" value="C:myosin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008307">
        <property type="term" value="F:structural constituent of muscle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007298">
        <property type="term" value="P:border follicle cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040011">
        <property type="term" value="P:locomotion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006936">
        <property type="term" value="P:muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031033">
        <property type="term" value="P:myosin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007424">
        <property type="term" value="P:open tracheal system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045214">
        <property type="term" value="P:sarcomere organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14909">
        <property type="entry name" value="MYSc_Myh1_insects_crustaceans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.820:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000036">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000008">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.530:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.360:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.4820:FF:000002">
        <property type="entry name" value="Myosin heavy chain 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000038">
        <property type="entry name" value="Myosin heavy chain muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000019">
        <property type="entry name" value="Myosin heavy chain, isoform G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000021">
        <property type="entry name" value="Myosin heavy chain, isoform G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000025">
        <property type="entry name" value="Myosin heavy chain, isoform G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000009">
        <property type="entry name" value="Myosin heavy chain, isoform G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000010">
        <property type="entry name" value="Myosin heavy chain, isoform G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000005">
        <property type="entry name" value="Myosin heavy chain, isoform I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000024">
        <property type="entry name" value="Myosin heavy chain, isoform J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.720:FF:000001">
        <property type="entry name" value="Myosin heavy chain, muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000050">
        <property type="entry name" value="Myosin heavy chain, muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.370">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.360">
        <property type="entry name" value="Myosin S1 fragment, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008989">
        <property type="entry name" value="Myosin_S1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002928">
        <property type="entry name" value="Myosin_tail"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004009">
        <property type="entry name" value="SH3_Myosin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014751">
        <property type="entry name" value="XRCC4-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615:SF27">
        <property type="entry name" value="MYOSIN HEAVY CHAIN, MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615">
        <property type="entry name" value="MYOSIN HEAVY CHAIN, NON-MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02736">
        <property type="entry name" value="Myosin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01576">
        <property type="entry name" value="Myosin_tail_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90257">
        <property type="entry name" value="Myosin rod fragments"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51844">
        <property type="entry name" value="SH3_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 14" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="6 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 14" id="KW-0505">Motor protein</keyword>
    <keyword evidence="10" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="8 14" id="KW-0518">Myosin</keyword>
    <keyword evidence="5 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0787">Thick filament</keyword>
    <feature type="domain" description="Myosin N-terminal SH3-like" evidence="17">
        <location>
            <begin position="34"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="Myosin motor" evidence="16">
        <location>
            <begin position="87"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="14">
        <location>
            <begin position="657"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1823"/>
            <end position="1842"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1920"/>
            <end position="1961"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="180"/>
            <end position="187"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010147-PK"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="20">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1961" mass="223847" checksum="CB445ACB2D67CA31" modified="2020-08-12" version="1">MPKPPVQVGEDPDPTEFLFVSLEQKRIDQSKPYDSKKACWVPEEKEGYVLGEIKATKGELVTVALPGGEEKNFKKEQLSQVNPPKFEKVEDMADLTYLNEAAVLHNLRQRYYSKLIYTYSGLFCVVINPYKRYPLYTNRCAKMYRGKRRNEVPPHLFAVSDGAYVNMLTNHENQSMLITGESGAGKTENTKKVIAYFATIGASGKKDENAEKKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFTGSGKLAGADIETYLLEKARVISQQTLERSYHIFYQIMSGSVKGLKEKCLLSNNIHDYHIVAQGKTTIPSVDDGEEMQITDEAFNVLGFTQEEKDNIYRITSAVMHMGRMQFKQKGREEQAEADGTEDGDRVAKLLGVGTDDLYKNLLKPRIKVGNEFVTKGQNKDQVTNSVGALCKGIFDRLFKWLVKKCNETLDTKQKRAQFIGVLDIAGFEIFDFNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGINWAFIDFGMDLLACVELIEKPMGILSILEEESMFPKATDQTFAEKLMTNHLGKSAPFMKPRPPKPGIPAGHFAIGHYAGVVSYNITGWLEKNKDPLNDTVVDQFKKGSNALMVEIFADHPGQSADPAAAKGGRGKKGAGFATVSSSYKEQLNNLMTTLKSTQPHFVRCIIPNEMKTAGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKLRYMILNPKGVEAEKDLKKCAQVIMDAAGLDSELYRLGNTKVFFRAGVLGQMEEFRDERLSKIMSWMQAWCRGYLSRKEFKKMQEQRVSLEIVQRNLRKYLKLRTWAWWKLWQKVKPLLNVSRVEDQIAKLEEKATKAQEAYEKEEKLRKELEALNSKLLAEKTALLDSLSGEKGALQEYQEKAAKLTAQKNDLENQLRDTQERLAQEEDARNQLFQTKKKLEQEIGSQKKDAEDLELQIQKIEQDKASKDHQIRNLNDEIAHQDELINKLNKEKKMQGEVNQKTAEELQAAEDKVNHLNKVKAKLEQTLDELEDSLEREKKLRGDVEKAKRKVEGDLKLTQEAVADLERNKKELEQTVLRKDKEISALSAKLEDEQSLVGKLQKQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELAKLRRDLEEANIQHEGTLANLRKKHNDAVAEMAEQVDQLNKLKTKAEKERTQYFAELNDARIGCDQLSNEKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEDAESQVSQLSKIKISLTQQLEDTKRLADEEARERATLLGKFRNLEHDLDNLREQVEEEAEGKGDIQRQLSKANAEAQLWRSKYESEGVARAEELEEAKRKLQARLAEAEETIESLNQKCIALEKTKQRLATEVEDLQLEVDRASSIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKSRKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDVQSALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELSDAHEQLNEVSAQNASIAAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAESNALKGGKKAIQKLEQRVRELESELDSEQRRHADAQKNLRKSERRIKELTFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADIAEQAATKFRTKGGRAGSVQRGASPAPQRQPSAMPALAGLNLPTFDDHGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8W239</accession>
    <name>A0A6E8W239_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Presynapse</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EnsemblMetazoa" id="ACON010286-RC">
        <property type="protein sequence ID" value="ACON010286-PC"/>
        <property type="gene ID" value="ACON010286"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_009032"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON010286"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_034840"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048788">
        <property type="term" value="C:cytoskeleton of presynaptic active zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098882">
        <property type="term" value="F:structural constituent of presynaptic active zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007274">
        <property type="term" value="P:neuromuscular synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048167">
        <property type="term" value="P:regulation of synaptic plasticity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019323">
        <property type="entry name" value="ELKS/CAST"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18861:SF0">
        <property type="entry name" value="BRUCHPILOT, ISOFORM J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18861">
        <property type="entry name" value="ELKS/RAB6-INTERACTING/CAST PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10174">
        <property type="entry name" value="Cast"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0770">Synapse</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="493"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="547"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1310"/>
            <end position="1339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1411"/>
            <end position="1435"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="99"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="163"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="732"/>
            <end position="1059"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="1116"/>
            <end position="1143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="504"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="549"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="563"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1318"/>
            <end position="1338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1411"/>
            <end position="1429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON010286-PC"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1685" mass="197262" checksum="26CCF3ED3AA4A74E" modified="2020-08-12" version="1">MGSPYYRDMDEPISPAGGHHRSRSATRAPTHSLEYPREQLRTRYQSLDRGLVDPHDREFIPIREPRDRSRDRSLERGLYLEEELYGRPPRQAMPERGYLGDLQVQNGELQRELGNLKKELELTNQKLGSSMHSIKTFWSPELKKERALRKEESAKYSLINDQLKLLNSENQKQAMLVRQLEEELRLRMRAPNIEMQQQMEALYAENEHLQREISILRDTIKELELRIETQKQTLQARDESIKKLLEMLQSKGMGKEEERQMFQQMQALAQKQVSLDDFRLEISRRDQEIMAMAAKMKTLEEQHQDYQRHIAVLKESLCAKEEHYNMLQSDVEELRNRLEEKNRMIEKKTQGTMHTVQEKNRLQTELTELKEHMDIKDRKINVLQRKIDNLEDLLKEKDNQVDMARARLSAMQAHHCSSEGALTSLEEAIGDKEKQMQQLRDQRDRAEAEKKEERELHERELAEFKMKLHSLDSEVEKLTTRLHRALAEKDRLEAKLESSQSELGKSKAELEHKTASDVGRSGADWDHAKQRLSRLELENERLRAELERSQTTFGRSTLTTSQELDRAQERADKTTNELRRTQAELRVTQTDAERSRAEAAALQEKLEKSQGEVYRLKAKLENAQGEQESLKQEMERGQAGIQRIVSERDKAYAELEKIREELERTQATLGKSQLTQEKLQNSLDKAQNEIDHLQEKLDKSGGETRRLQLEKEKINYEFENIQSQLDKSLGQASRIQKEKEAAQMDLDRYRDKADKLQSSLARLQKERDVLSDELEKLKEKSESTQSTALKYQRERDAIQTELEVVKERWEKAHQVHQKLQMERDDATTEIDILKEKLDKALYASQKLIDEKDTSTKEFEKMLEKYDRAQNEIYRLQSRVDTAEADRNRLEVEAERSALAAAKAREDLRKIQEETTRLQEACDRAAMQLGRSKELEDKAKEDVDMIRERLDKCQTDLRRAQSEKDNQQAEIERLTYELDRSHNLYTKQTASLDSAQEEIARYGLEIEKMRERYEKTAAELRRLQENESFSREARRMKDENDRLREKYDKVIVELENFRGKSQYEQETFDKMKEKFETRENEFQTMELKLHETSLQLDLARAEVTKLISNQDKQRADAERAHIEYEKIRDKHEKLIKEVERLRQNPQSAISPGALAATSTVSLADKNELDRLRDRLEKALQSRDATEMEAGRLAKELEKAQLHLTKQQEVYEATRIEFERMSAELTRVLERLEKSEAEKETLRQSTKIYEKHHQQHASNHHIEQSLMKLEADNKQLMAERDQLVMQLEKSQDMLMSFQQELNAAEVELQRQCEENRRLKSSPQQPGSAPAAPQQGQAAAQEIQNYKKEITKLKQMLQETGARGDNELEQWRKVVEQEKNRADQAEKAAIELQKRMQVMEQQLKQQLLQMTNMQKQLQQQSTQQQPPQQSPSNKKELEKLQEDLKTAAAQRDQFQNQLELLVQELEKSQTEALEATKKTQQQQQQVGQLQQQIQQMQQQLQQATQAANQKVAQNAGVSEAERKQLEAQMKQIEDATRLLESERKTFEDQRKAIENKRKELEDKEKNLIEFDKQLKKRKEQMDQLEKSLQKAGGTAAAAGELNKQLTETQQMLDKASKELEDAKEEAKRSAAETERLLQLVQMTQEEQNQKEKTIMDLQQALKNAQAKLKTAQAQPQDAGPAGFLKSFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WA07</accession>
    <name>A0A6E8WA07_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="4">DMPK_coil domain-containing protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">Ngousso</strain>
        </source>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A6E8WA07"/>
    <dbReference type="EnsemblMetazoa" id="ACON029453-RA">
        <property type="protein sequence ID" value="ACON029453-PA"/>
        <property type="gene ID" value="ACON029453"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="ACON029453-RB">
        <property type="protein sequence ID" value="ACON029453-PB"/>
        <property type="gene ID" value="ACON029453"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_002179"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON029453"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_033456"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051293">
        <property type="entry name" value="MTUS1/CCDC69"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24200">
        <property type="entry name" value="TOUCAN, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24200:SF11">
        <property type="entry name" value="TOUCAN, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="94"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="193"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="320"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="840"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="973"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1023"/>
            <end position="1194"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="448"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="515"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="604"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="48"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="68"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="332"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1077"/>
            <end position="1113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1117"/>
            <end position="1129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON029453-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="1194" mass="128868" checksum="E3766D58576EEFC2" modified="2020-08-12" version="1">MGAHGSKEKLSRSASERYVHTQVIERERFGSFGKRGRGGPAKKRDVRTINSSSSDLGPPSKTNGAQPARSSAARAAAALQKRISPNLTSASIPLVPEQLKASPPTKRNMLLKRNGTTNGTLPAKTPLKGKLAAHLLKDKESNKENSINGDHNRLAGATATTKSAAAVATAGTVASSNHSPKPTLAKSTLLRSTSAVPTTATNHHATPPPPSHLSHHPQHAVNGSGGSGGVANNTTTASAASNATVKAIQRNGTIILDKSSSPLLICEVGERAKGVAAGANSATSTPEVQSILTILPSACSTPLVATAGTPVAAGKEPNCDQLHQQQHRNGRHRETAPHHSESKGSVDLAMLNHAQKGLDAFSCPTIKAAHQKTSADLLETKSILDEERTNSQTLKQQLLETTERETELQELHRCELSKVETCLSEQQRSADDRIAALEVQLLAKESHSRELLARIDGYEAKLADQAEQLESARARELDLLQRINALSCTENELRDKVHSSELAFSERLQAAAMRERDLTDQINGLHREMERMRRETERKERELEEKLTITKDECVVLRQSRNGSVEPACSQTGSGTAAASSGNMMNVSGNPLQLNRSSGNINHLQVLQDEVDSLRCVLDLKQREISELRKQTQEYERDAKDLPGALTKISALESRIEDLEVQLKTKTEEEKELQQKLKHLQDNLNQETKIKSRLSLHNEELQWRLKQNSEKFTLAYAELSKSYHENSSFMMNATKSSLDQSVSHHQHQLSHNQSRSSCSDRFFDMDDISPPTSPVIKGMVEKSDSVSWVLEIDDEPPEVSASRMVRRAGSFRSSAAYDSVAKRPKCAGLNQTTSGIQQSSSAASILKQHSSDLSAAAVKPPHSGAPSHRLRSKSVSIKAAEPPKKIVRSNSGASASPCPPAGVPCKPSLRMVDLPGSSWKEQPLYSSSPYAHKRYLTEELDLIGTGKPFAEQQQQHHNRAKDLFLEETEFLSEPDSPFARCPPNEAGEANMADPPVEIFHRDKTPHFKKSKENRGLITCDTTALMGGVGSSSKASGSGGALTAREKRREFTRNAGSALKEGAGEALVSGSNSDDEALSTSSSSSGSSSSCISEASLSSATGSSSSSHDNSPLHSGKRSSERMNNEHDDTVEGAANSSDEHPSQHRRRHSNVSLEDALMKKIVASLNGGGDEPHDGTPMEVSWSEDGDPGSESNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6E8WC41</accession>
    <name>A0A6E8WC41_ANOCL</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Broad</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Anopheles coluzzii</name>
        <name type="common">African malaria mosquito</name>
        <dbReference type="NCBI Taxonomy" id="1518534"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Nematocera</taxon>
            <taxon>Culicoidea</taxon>
            <taxon>Culicidae</taxon>
            <taxon>Anophelinae</taxon>
            <taxon>Anopheles</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2019" name="Genes (Basel)" volume="10" first="0" last="E62">
            <title>A High-Quality De novo Genome Assembly from a Single Mosquito Using PacBio Sequencing.</title>
            <authorList>
                <person name="Kingan S.B."/>
                <person name="Heaton H."/>
                <person name="Cudini J."/>
                <person name="Lambert C.C."/>
                <person name="Baybayan P."/>
                <person name="Galvin B.D."/>
                <person name="Durbin R."/>
                <person name="Korlach J."/>
                <person name="Lawniczak M.K.N."/>
            </authorList>
            <dbReference type="PubMed" id="30669388"/>
            <dbReference type="DOI" id="10.3390/genes10010062"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mali-NIH</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2020-05" db="UniProtKB">
            <authorList>
                <consortium name="EnsemblMetazoa"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">Ngousso</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A6E8WC41"/>
    <dbReference type="EnsemblMetazoa" id="ACON029491-RA">
        <property type="protein sequence ID" value="ACON029491-PA"/>
        <property type="gene ID" value="ACON029491"/>
    </dbReference>
    <dbReference type="VEuPathDB" id="VectorBase:ACMO_010334"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON029491"/>
    <dbReference type="VEuPathDB" id="VectorBase:ACON2_035074"/>
    <dbReference type="Proteomes" id="UP001105220">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048813">
        <property type="term" value="P:dendrite morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030707">
        <property type="term" value="P:follicle cell of egg chamber development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061061">
        <property type="term" value="P:muscle structure development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007423">
        <property type="term" value="P:sensory organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18315">
        <property type="entry name" value="BTB_POZ_BAB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000118">
        <property type="entry name" value="Abrupt, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051095">
        <property type="entry name" value="Dros_DevTransReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110:SF99">
        <property type="entry name" value="BROAD-COMPLEX CORE PROTEIN ISOFORM 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110">
        <property type="entry name" value="BTB DOMAIN TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="8">
        <location>
            <begin position="32"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="495"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="525"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="154"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="206"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="348"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="165"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="177"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="206"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="244"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="348"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="374"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="392"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="422"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EnsemblMetazoa" id="ACON029491-PA"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001105220"/>
        </source>
    </evidence>
    <sequence length="563" mass="60321" checksum="5EDE514FAF67EF64" modified="2020-08-12" version="1">MVDTQHFCLRWNNYQSSITSAFENLRDDEDFVDVTLACDGRSLKAHRVVLSACSTYFRELLKSTPCKHPVIVLQDVAFTDLHALVEFIYHGEVNVHQRSLSSFLKTAEILRVSGLTQQQAEETHGMHASLGANLPSGVVHHPIYPEKMLDDSLYVSQGASPPPHLSSLHNSSSPNSGGVGGGNSNGGGAPMVNQLLKRAAAAAALRRERNNSVHSDEMALKRHRMSVDSNGPTNPRDHLDVICSTPQTTATDFSSSAAKQHLSLPSPQGKDQMRQQDSLSGHANSGSNSFSINSASNLIKSSINNNNNNINNNNNLNNNLLHSKENSGVGGGGAGGLCGSGGSMPTSLGTSNGSNAGSLCSAEKESLASSPSERSAEDVKSEPLELLCGTGGDHENSSDSVTDDHGDLVKGGLDVKGSLRSPNDHELESSMHHHSGAQFLMNASENKLFPTPPSFNFSMAALAADPSALAGFSTQALQAVELAGSPQGCSPKKLFSCQLCGKVLCSKASLKRHIADKHAERQEEYRCIICERVYCSRNSLMTHIYTYHKSRPGELDMKDVKFF</sequence>
</entry>
<copyright>
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms Distributed under the Creative Commons Attribution (CC BY 4.0) License
</copyright>
</uniprot>